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IL2 — RAF1
Pathways - manually collected, often from reviews:
-
NCI Pathway Database IL2-mediated signaling events:
RAF1 (RAF1)
→
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1)
(modification, activates)
Blanchard et al., Oncogene 2000, Lundin Brockdorff et al., Cytokine 2002, Karnitz et al., Mol Cell Biol 1995
Evidence: mutant phenotype, other species
-
NCI Pathway Database SHP2 signaling:
RAF1 (RAF1)
→
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1)
(modification, activates)
Blanchard et al., Oncogene 2000, Lundin Brockdorff et al., Cytokine 2002, Karnitz et al., Mol Cell Biol 1995
Evidence: mutant phenotype
Text-mined interactions from Literome
Maslinski et al., J Biol Chem 1992
:
Interleukin-2 (IL-2) induces tyrosine kinase dependent translocation of active
raf-1 from the IL-2 receptor into the cytosol
Turner et al., Proc Natl Acad Sci U S A 1991
:
Furthermore, much of the
IL-2 stimulated phosphorylation of p72-74
Raf-1 occurred on tyrosines
Turner et al., Proc Natl Acad Sci U S A 1993
:
Interleukin 2 regulates
Raf-1 kinase activity through a tyrosine phosphorylation dependent mechanism in a T-cell line ... These findings provide evidence that the
IL-2 stimulated phosphorylation of
Raf-1 on tyrosines plays an important role in upregulating the activity of this serine/threonine-specific kinase in CTLL-2 cells and, as such, provides a model system for studying the transfer of growth factor initiated signals from protein tyrosine kinases to serine/threonine-specific kinases
Owaki et al., EMBO J 1993
:
Raf-1 is
required for T cell
IL2 production ... The
effects of
Raf-1 on
interleukin 2 (IL2) production in T cells were examined using activated and inhibitory forms of Raf-1 ... This increase in
Raf-1 expression allowed antibodies to CD3 and to CD28 to stimulate IL2 production in the absence of phorbol myristate acetate ( PMA ) and
enhanced IL2 production stimulated by these antibodies in the presence of PMA ... We conclude that
Raf-1 activity is
necessary for
IL2 gene transcription and secretion
Whitehurst et al., J Immunol 1996
:
Raf-1 activity
promotes IL-2 production in activated T lymphocytes ... Expression of constitutively active
Raf-1 or MEK1 in Jurkat T cells
enhanced the stimulation of
IL-2 promoter-driven transcription stimulated by a calcium ionophore and PMA, and together with a calcium ionophore the expression of each protein was sufficient to stimulate NF-AT activity
Barber et al., Blood 1997
:
In addition, EPO,
IL-2 , and IL-15
activated Raf1 and ERK2, demonstrating that the Raf1/MEK/MAP kinase pathway was activated