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CDKN1B — RHOA

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: RHOA → CDKN1B (directlyDecreases, CDKN1B Activity)
  Evidence: p27 binds to RhoA and prevents the interaction of RhoA with its activators the guanine-nucleotide exchange factors. Fibroblasts lacking p27 have impaired migration. CIP1 binds to and inhibits Rho kinases (ROCK1 and -2) - downstream effectors of Rho. p57 (KIP2) binds to and targets LIM domain-containing protein kinase (LIMK) to the nucleus, sequestering it in a compartment where it cannot regulate the actin cytoskeleton.
• OpenBEL Selventa BEL large corpus: RHOA → CDKN1B (decreases, CDKN1B Activity, RHOA Translocation)
  Evidence: p27 binds to RhoA and prevents the interaction of RhoA with its activators the guanine-nucleotide exchange factors. Fibroblasts lacking p27 have impaired migration. CIP1 binds to and inhibits Rho kinases (ROCK1 and -2) - downstream effectors of Rho. p57 (KIP2) binds to and targets LIM domain-containing protein kinase (LIMK) to the nucleus, sequestering it in a compartment where it cannot regulate the actin cytoskeleton.
• BioCarta influence of ras and rho proteins on g1 to s transition: RhoA (RHOA) → p27 (CDKN1B) (modification, activates)
• NCI Pathway Database Regulation of RhoA activity: p27Kip1 (CDKN1B) → RHOA/GDP complex (RHOA) (modification, inhibits) Besson et al., Genes Dev 2004*, Dubash et al., J Cell Sci 2007*, Hart et al., Science 1998*
  Evidence: assay, physical interaction
• NCI Pathway Database Regulation of RhoA activity: p27Kip1 (CDKN1B) → RHOA/GDP complex (RHOA) (modification, inhibits) Besson et al., Genes Dev 2004*, Baisamy et al., J Biol Chem 2005*, Glaven et al., J Biol Chem 1996*
  Evidence: assay, physical interaction
• NCI Pathway Database RhoA signaling pathway: RHOA/GTP complex (RHOA) → p27Kip1 (CDKN1B) (modification, activates) Hu et al., J Biol Chem 1999*
  Evidence: assay

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: CDKN1B — RHOA (physical association, affinity chromatography technology) Larrea et al., Proc Natl Acad Sci U S A 2009*
• IRef Dip Interaction: CDKN1B — RHOA (direct interaction, anti bait coimmunoprecipitation) Larrea et al., Proc Natl Acad Sci U S A 2009*
• IRef Dip Interaction: CDKN1B — RHOA (direct interaction, pull down) Larrea et al., Proc Natl Acad Sci U S A 2009*

Text-mined interactions from Literome

Konecny et al., Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences 2010 : After transport in cytoplasm, p27 Kip1 binds to RhoA thus prevent activation of RhoA by GEFs, thus inhibit GDP-GTP exchange mediated by GEFs
Auer et al., Biochem J 1998 : Inhibition of RhoA GTPase function increased the basal expression of p21Cip-1/WAF1 and p27Kip-1 but not p16INK4a, and enhanced the ability of MAPK signalling to decrease DNA synthesis