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EGF — GRB2

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: GRB2 → EGF (increases) Olsen et al., Cell 2006*
  Evidence: We have detected 6,600 phosphorylation sites on 2,244 proteins and have determined their temporal dynamics after stimulating HeLa cells with epidermal growth factor (EGF) and recorded them in the Phosida database
• BioCarta role of egf receptor transactivation by gpcrs in cardiac hypertrophy: EGF/EGF R complex (EGF-EGFR) → SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1) (modification, activates)
  
• BioCarta egf signaling pathway: EGF/EGF-R complex (EGF-EGFR) → SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1) (modification, activates)
  
• BioCarta cbl mediated ligand-induced downregulation of egf receptors pathway: EGF R/SRC/EGF complex (EGFR-SRC-EGF) → GRB2 (protein ubiquitination, collaborate)
• BioCarta keratinocyte differentiation: EGF/EGF-R complex (EGF-EGFR) → SHC/GRB2/SOS-1 complex (SOS1-GRB2-SHC1) (modification, activates)
  
• NCI Pathway Database EGFR-dependent Endothelin signaling events: EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1) → mTOR (MTOR) (modification, activates) Iwasaki et al., Endocrinology 1999
  Evidence: mutant phenotype, other species
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF complex (EGFR-EGF) → GRB2/SOS1 complex (GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Pennock et al., Mol Cell Biol 2003
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF complex (EGFR-EGF) → EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Pennock et al., Mol Cell Biol 2003
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: GRB2/SOS1 complex (GRB2-SOS1) → EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Pennock et al., Mol Cell Biol 2003
  Evidence: assay
• NCI Pathway Database Signaling events mediated by TCPTP: TCPTP p45 (PTPN2) → EGFR/EGFR/EGF/EGF/GRB2/GAB1 complex (EGFR-EGF-GRB2-GAB1) (modification, activates)
  Tiganis et al., J Biol Chem 1999*, Lam et al., J Biol Chem 2001*, Tiganis et al., Mol Cell Biol 1998
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database EGFR-dependent Endothelin signaling events: EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1) → EGF/EGFR dimer/SHC complex (EGF-EGFR-SHC1) (modification, collaborate)
  Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
  Evidence: physical interaction, other species
• NCI Pathway Database EGFR-dependent Endothelin signaling events: EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1) → GRB2/SOS1 complex (GRB2-SOS1) (modification, collaborate)
  Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
  Evidence: physical interaction, other species
• NCI Pathway Database EGFR-dependent Endothelin signaling events: EGF/EGFR dimer/SHC complex (EGF-EGFR-SHC1) → GRB2/SOS1 complex (GRB2-SOS1) (modification, collaborate)
  Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
  Evidence: physical interaction, other species
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/CBL complex (EGFR-EGF-GRB2-CBL) → EGFR/EGFR/EGF/EGF complex (EGFR-EGF) (modification, collaborate)
  
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/CBL complex (EGFR-EGF-GRB2-CBL) → GRB2/CBL complex (GRB2-CBL) (modification, collaborate)
  
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF complex (EGFR-EGF) → GRB2/CBL complex (GRB2-CBL) (modification, collaborate)
  
• NCI Pathway Database ErbB1 downstream signaling: GRB2 (GRB2) → EGFR/EGFR/EGF/EGF complex (EGFR-EGF) (modification, inhibits)
  Zhang et al., Biochem J 2003*, Shi et al., J Biol Chem 2004*, Sadowski et al., Nature 1993*, Coffer et al., Biochem Biophys Res Commun 1995*, Ruff-Jamison et al., J Biol Chem 1994*, Zhong et al., Science 1994, Ruff-Jamison et al., Science 1993*, Silvennoinen et al., Science 1993*, Sadowski et al., Science 1993*, Leaman et al., Mol Cell Biol 1996*, David et al., J Biol Chem 1996*, Park et al., Proc Natl Acad Sci U S A 1996*
  Evidence: assay, physical interaction
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF complex (EGFR-EGF) → EGFR/EGFR/EGF/EGF/GRB2/CBL complex (EGFR-EGF-GRB2-CBL) (modification, collaborate)
  Levkowitz et al., Mol Cell 1999, Waterman et al., EMBO J 2002, Jiang et al., Mol Biol Cell 2003*, Jiang et al., Traffic 2003*, Huang et al., Mol Biol Cell 2005, Huang et al., Proc Natl Acad Sci U S A 2007*
  Evidence: physical interaction
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF complex (EGFR-EGF) → GRB2/CBL complex (GRB2-CBL) (modification, collaborate)
  Levkowitz et al., Mol Cell 1999, Waterman et al., EMBO J 2002, Jiang et al., Mol Biol Cell 2003*, Jiang et al., Traffic 2003*, Huang et al., Mol Biol Cell 2005, Huang et al., Proc Natl Acad Sci U S A 2007*
  Evidence: physical interaction
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/CBL complex (EGFR-EGF-GRB2-CBL) → GRB2/CBL complex (GRB2-CBL) (modification, collaborate)
  Levkowitz et al., Mol Cell 1999, Waterman et al., EMBO J 2002, Jiang et al., Mol Biol Cell 2003*, Jiang et al., Traffic 2003*, Huang et al., Mol Biol Cell 2005, Huang et al., Proc Natl Acad Sci U S A 2007*
  Evidence: physical interaction
• NCI Pathway Database EGFR-dependent Endothelin signaling events: None → EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1) (modification, collaborate)
  Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
  Evidence: mutant phenotype, other species
• NCI Pathway Database EGFR-dependent Endothelin signaling events: HRAS/GDP complex (HRAS) → EGF/EGFR dimer/SHC/GRB2/SOS1 complex (EGF-EGFR-SHC1-GRB2-SOS1) (modification, collaborate)
  Iwasaki et al., Endocrinology 1999, Daub et al., Nature 1996
  Evidence: mutant phenotype, other species
• NCI Pathway Database Internalization of ErbB1: GRB2 (GRB2) → EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) (modification, collaborate)
  Burke et al., Mol Biol Cell 2001
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: GRB2 (GRB2) → EGF (EGF) (modification, collaborate) Burke et al., Mol Biol Cell 2001
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) → EGF (EGF) (modification, collaborate) Burke et al., Mol Biol Cell 2001
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) → EGFR (EGFR) (modification, collaborate) Burke et al., Mol Biol Cell 2001
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) → SOS1 (SOS1) (modification, collaborate) Burke et al., Mol Biol Cell 2001
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: None → EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Pennock et al., Mol Cell Biol 2003, Wiley et al., J Biol Chem 1991, Di Guglielmo et al., EMBO J 1994, Herbst et al., J Biol Chem 1994
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: RAS family/GDP complex (HRAS_KRAS_HRAS_KRAS_NRAS) → EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Pennock et al., Mol Cell Biol 2003, Wiley et al., J Biol Chem 1991, Di Guglielmo et al., EMBO J 1994, Herbst et al., J Biol Chem 1994
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) → RAS family/GTP complex (HRAS_KRAS_HRAS_KRAS_NRAS) (modification, activates)
  Haugh et al., J Biol Chem 1999, Pennock et al., Mol Cell Biol 2003, Wiley et al., J Biol Chem 1991, Di Guglielmo et al., EMBO J 1994, Herbst et al., J Biol Chem 1994
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) → None (modification, activates) Haugh et al., J Biol Chem 1999, Pennock et al., Mol Cell Biol 2003, Wiley et al., J Biol Chem 1991, Di Guglielmo et al., EMBO J 1994, Herbst et al., J Biol Chem 1994
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: None → EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: RAS family/GDP complex (HRAS_KRAS_HRAS_KRAS_NRAS) → EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) → RAS family/GTP complex (HRAS_KRAS_HRAS_KRAS_NRAS) (modification, activates)
  Haugh et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) → None (modification, activates) Haugh et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/p46 SHC complex (EGFR-EGF-SHC1) → GRB2/SOS1 complex (GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
  Evidence: assay, physical interaction
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/p46 SHC complex (EGFR-EGF-SHC1) → EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
  Evidence: assay, physical interaction
• NCI Pathway Database Internalization of ErbB1: GRB2/SOS1 complex (GRB2-SOS1) → EGFR/EGFR/EGF/EGF/p46 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
  Evidence: assay, physical interaction
• NCI Pathway Database Internalization of ErbB1: None → EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: RAS family/GDP complex (HRAS_KRAS_HRAS_KRAS_NRAS) → EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) → None (modification, activates) Haugh et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database Internalization of ErbB1: Vps24 (CHMP3) → EGFR/EGFR/EGF/EGF/GRB2/SOS1 complex (EGFR-EGF-GRB2-SOS1) (translocation, activates)
  Burke et al., Mol Biol Cell 2001, Ravid et al., J Biol Chem 2004, Mosesson et al., Dev Cell 2009, Levkowitz et al., Genes Dev 1998
  Evidence: assay
• NCI Pathway Database SHP2 signaling: SHP2 (PTPN11) → EGFR/EGFR/EGF/EGF/GRB2/GAB1 complex (EGFR-EGF-GRB2-GAB1) (modification, activates)
  Zhang et al., Mol Cell Biol 2002*, Montagner et al., J Biol Chem 2005
  Evidence: mutant phenotype, assay, physical interaction, other species
• NCI Pathway Database Internalization of ErbB1: GRB2/SOS1 complex (GRB2-SOS1) → EGFR/EGFR/EGF/EGF/p52 SHC complex (EGFR-EGF-SHC1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
  Evidence: assay, physical interaction
• NCI Pathway Database Internalization of ErbB1: GRB2/SOS1 complex (GRB2-SOS1) → EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
  Evidence: assay, physical interaction
• NCI Pathway Database Internalization of ErbB1: EGFR/EGFR/EGF/EGF/p52 SHC complex (EGFR-EGF-SHC1) → EGFR/EGFR/EGF/EGF/p52 SHC/GRB2/SOS1 complex (EGFR-EGF-SHC1-GRB2-SOS1) (modification, collaborate)
  Haugh et al., J Biol Chem 1999, Balbis et al., Endocrinology 2007, Di Guglielmo et al., EMBO J 1994
  Evidence: assay, physical interaction
• Reactome Reaction: EGF → GRB2 (direct_complex) Rosenthal et al., J Biol Chem 1999, Soubeyran et al., Nature 2002, Kassenbrock et al., J Biol Chem 2004, Galisteo et al., J Biol Chem 1995*
• Reactome Reaction: EGF → GRB2 (reaction) Joazeiro et al., Science 1999, Gual et al., Oncogene 2000, Lock et al., J Biol Chem 2000*, Waterman et al., EMBO J 2002, Agazie et al., Mol Cell Biol 2003, Marmor et al., Oncogene 2004, Jackson et al., Cancer Res 2004, Kassenbrock et al., J Biol Chem 2004, Lynch et al., N Engl J Med 2004, Fan et al., J Biol Chem 2004*, Sordella et al., Science 2004, Pao et al., Proc Natl Acad Sci U S A 2004, Greulich et al., PLoS Med 2005, Yang et al., Cancer Res 2006, Choi et al., Oncogene 2007, Lee et al., PLoS Med 2006, Huang et al., Proc Natl Acad Sci U S A 2007, Xu et al., Br J Cancer 2007, Batzer et al., Mol Cell Biol 1994*, Okutani et al., J Biol Chem 1994*, Galisteo et al., J Biol Chem 1995*, Chardin et al., Science 1993, Xie et al., J Biol Chem 1995, Holgado-Madruga et al., Proc Natl Acad Sci U S A 1997, Lehr et al., Biochemistry 1999*
• Reactome Reaction: EGF → GRB2 (indirect_complex) Joazeiro et al., Science 1999, Rodrigues et al., Mol Cell Biol 2000, Gual et al., Oncogene 2000, Lock et al., J Biol Chem 2000*, Cunnick et al., J Biol Chem 2001*, Waterman et al., EMBO J 2002, Agazie et al., Mol Cell Biol 2003, Kapoor et al., Mol Cell Biol 2004*, Marmor et al., Oncogene 2004, Jackson et al., Cancer Res 2004, Lynch et al., N Engl J Med 2004, Fan et al., J Biol Chem 2004*, Sordella et al., Science 2004, Pao et al., Proc Natl Acad Sci U S A 2004, Greulich et al., PLoS Med 2005, Yang et al., Cancer Res 2006, Choi et al., Oncogene 2007, Lee et al., PLoS Med 2006, Huang et al., Proc Natl Acad Sci U S A 2007, Xu et al., Br J Cancer 2007, Batzer et al., Mol Cell Biol 1994*, Okutani et al., J Biol Chem 1994*, Chardin et al., Science 1993, Xie et al., J Biol Chem 1995, Holgado-Madruga et al., Proc Natl Acad Sci U S A 1997, Lehr et al., Biochemistry 1999*

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Hprd Interaction: EGF — GRB2 (in vivo) Kong et al., J Biol Chem 2000*
• IRef Ophid Interaction: EGF — GRB2 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Tan et al., Proc Natl Acad Sci U S A 1999 : Moreover, the coupling of NS5A to Grb2 in these cells was induced by epidermal growth factor stimulation
Takeuchi et al., Am J Physiol 1999 : Since Ras activation requires the assembly of a multiprotein complex, we examined the effect of carbachol and EGF on tyrosyl phosphorylation of Shc and its association with Grb2 and the guanine nucleotide exchange factor Sos
Wang et al., J Biol Chem 1999 : The tyrphostin AG1478, known to inhibit EGF receptor phosphorylation, also inhibited the angiotensin II and EGF induced activation of ERK, the phosphorylation of the EGF receptor, and the subsequent association of Shc and Grb2 ... Nitric oxide did not affect either EGF receptor phosphorylation or Shc-Grb2 activation induced by either Ang II or EGF
Katayama et al., Jpn J Cancer Res 1999 (Glioma) : By contrast, Shc and Grb2 were inducibly associated with the EGFR in response to EGF stimulation of glioma cells
Take et al., Biochem Biophys Res Commun 2000 : Our results suggest that CIN85 may play a specific role in the EGF receptor mediated signaling cascade via its interaction with c-Cbl
He et al., J Virol 2002 : NS5A containing mutations within the C-terminal proline-rich motif neither bound Grb2 nor inhibited ERK1/2 activation by EGF , demonstrating that NS5A-Grb2 binding and downstream effects were due to direct interactions
Kowanetz et al., J Biol Chem 2003 : Identification of a novel proline-arginine motif involved in CIN85 dependent clustering of Cbl and down-regulation of epidermal growth factor receptors
Choi et al., Cell Signal 2005 : Replacement of Tyr783 with Phe completely blocked EGF induced interaction of PLC-gamma1 with Grb2 , indicating that tyrosine phosphorylation of PLC-gamma1 at Tyr783 is essential for the interaction with Grb2
Gosens et al., Am J Physiol Lung Cell Mol Physiol 2007 : EGF induced concomitant phosphorylation of caveolin-1 and p42/p44 MAP kinase ; however, EGF did not promote the localization of p42/p44 MAP kinase, Grb2 , or Raf-1 to caveolae
Cao et al., Cancer Lett 2008 (Ovarian Neoplasms) : Further studies showed that PD153035, which does not reverse ligand induced EGFR down-regulation, blocks EGF induced EGFR activation as well as EGFR 's binding to c-cbl and Grb2
Ayoub et al., PloS one 2013 : Both EGF and HRG effects on Grb2 interaction are dose dependent, and specifically blocked by EGFR inhibitor AG-1478 ... Furthermore, truncation of HER3 to remove the putative Grb2 binding sites appears to abolish EGF induced Grb2 recruitment to the EGFR-HER3 heteromer ... Our results support the concept that EGFR interacts with Grb2 in both constitutive and EGF dependent manners and this interaction is independent of HER3 co-expression
Polk et al., Gastroenterology 1995 : EGF induced association of Shc with the adapter protein Grb2 may have implications for activation of the p21ras signaling pathway in the intestine
Yamauchi et al., J Biol Chem 1995 (Carcinoma, Hepatocellular...) : Further, activation of the EGF receptor did not result in any significant tyrosine phosphorylation of SHPTP2 and/or the association of the 115-kDa protein with Grb2
Hashimoto et al., Oncogene 1994 : Both NGF and EGF induced rapid tyrosine phosphorylation of Shc and its association with both the receptors and with Grb2/Ash
Ohmichi et al., J Biol Chem 1994 : NGF and EGF also induced the association of Shc proteins with a Grb2 fusion protein or endogenous Grb2 , whereas insulin had no effect
Tanaka et al., Mol Cell Biol 1995 : In contrast to results with Abl, Erk activation by EGF was strongly inhibited only by Grb2 mutants ; Crk and Nck mutants had little or no effect
Satoh et al., FEBS Lett 1996 : Furthermore, we observed association of ACK with an adaptor protein Grb2, which may suggest the involvement of Grb2 in EGF receptor mediated tyrosine phosphorylation of ACK
Wang et al., Science 1996 : EGF stimulation of canine epithelial cells induced a rapid and transient association of the SH3-SH2-SH3 protein GRB2 with dynamin, a guanosine triphosphatase that regulates endocytosis
Wong et al., J Biol Chem 1996 : Additional experiments indicated that neither the binding of the nucleotide exchange factor Sos to GRB2 nor tyrosine phosphorylation of PTP1D was required for EGF stimulated coupling of PTP1D to GRB2
Johnson et al., J Biol Chem 1996 : Because Ras activation by EGF involves EGF stimulated association of EGF-R with Grb2 , the EGF-R was immunoprecipitated and a Western blot was probed for Grb2
Anafi et al., J Biol Chem 1997 : Epidermal growth factor (EGF) stimulation did not affect the binding of Grb2 to HPK1 but induced recruitment of the Grb2.HPK1 complex to the autophosphorylated EGF receptor and to the Shc docking protein
Tiganis et al., Mol Cell Biol 1998 : However, TC45 inhibited the EGF induced association of p52Shc with Grb2 , which was attributed to the ability of the PTP to recognize specifically p52Shc phosphorylated on Y239
Deb et al., J Biol Chem 1998 : Dominant negative forms of SHP-2 had no effect on EGF stimulated interaction of GRB2 with EGFR or SHC, nor did they influence phosphorylation of SHC and SHC/EGFR association
Gresham et al., Int J Cancer 1998 (Prostatic Neoplasms) : In all of the tumor cell lines, EGF caused an association between Shc and Grb2 , another adapter protein linked to cellular ras activation