Gene interactions and pathways from curated databases and text-mining

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NFKB1 — RELA

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Badrichani et al., J Clin Invest 1999 : In addition, Bcl-2 or Bcl-XL inhibits activation of NF-kappaB and thus upregulation of proinflammatory genes ... Bcl-2 mediated inhibition of NF-kappaB in EC occurs upstream of IkappaBalpha degradation without affecting p65 mediated transactivation
Kol et al., J Clin Invest 1999 : We also tested whether either HSP 60 induces nuclear factor-kappaB (NF-kappaB) , which contributes to the gene expression of these molecules ... In ECs, either HSP 60 triggered activation of NF-kappaB complexes containing p65 and p50 Rel proteins
Nomura et al., Toxicol Lett 1998 (Ischemic Attack, Transient) : LPS caused intranuclear translocation of NF-kappaB , and IFNgamma induced phosphorylation of Jak2 and Stat1 , followed by the translocation of Stat1 into the nucleus ... LPS caused intranuclear translocation of NF-kappaB , and IFNgamma induced phosphorylation of Jak2 and Stat1, followed by the translocation of Stat1 into the nucleus ... LPS caused intranuclear translocation of NF-kappaB , and IFNgamma induced phosphorylation of Jak2 and Stat1, followed by the translocation of Stat1 into the nucleus ... LPS caused intranuclear translocation of NF-kappaB , and IFNgamma induced phosphorylation of Jak2 and Stat1, followed by the translocation of Stat1 into the nucleus
Bonizzi et al., Mol Cell Biol 1999 (Adenocarcinoma) : Reactive oxygen intermediate dependent NF-kappaB activation by interleukin-1beta requires 5-lipoxygenase or NADPH oxidase activity ... Reactive oxygen intermediate dependent NF-kappaB activation by interleukin-1beta requires 5-lipoxygenase or NADPH oxidase activity ... Reactive oxygen intermediate dependent NF-kappaB activation by interleukin-1beta requires 5-lipoxygenase or NADPH oxidase activity ... Stimulation of lymphoid cells with interleukin-1beta (IL-1beta) led to ROI production and NF-kappaB activation, which could both be blocked by antioxidants or FLAP inhibitors, confirming that 5-LOX was the source of ROIs and was required for NF-kappaB activation in these cells ... Stimulation of lymphoid cells with interleukin-1beta (IL-1beta) led to ROI production and NF-kappaB activation, which could both be blocked by antioxidants or FLAP inhibitors, confirming that 5-LOX was the source of ROIs and was required for NF-kappaB activation in these cells ... However, reintroduction of a functional 5-LOX system in these cells allowed ROI production and 5-LOX dependent NF-kappaB activation ... This pathway involves the Rac1 and Cdc42 GTPases, two enzymes which are not required for NF-kappaB activation by IL-1beta in epithelial cells
Chan et al., Mol Cell Biol 1999 : Activation dependent transcriptional regulation of the human Fas promoter requires NF-kappaB p50-p65 recruitment
Lallena et al., Mol Cell Biol 1999 : Previous studies have demonstrated that the atypical PKCs are stimulated by tumor necrosis factor alpha (TNF-alpha) and are required for the activation of NF-kappaB by this cytokine through a mechanism that most probably involves the phosphorylation of IkappaB
Novick et al., Immunity 1999 : IL-18BP abolished IL-18 induction of interferon-gamma (IFNgamma) , IL-8, and activation of NF-kappaB in vitro ... IL-18BP abolished IL-18 induction of interferon-gamma (IFNgamma), IL-8, and activation of NF-kappaB in vitro
Chariot et al., J Biol Chem 1999 : In this report, we demonstrated that the HOXB7 homeodomain containing protein, which plays a key role in development and differentiation, physically interacted in vitro with IkappaB-alpha , an inhibitor of NF-kappaB activity
Ogretmen et al., Biochemistry 1999 (Breast Neoplasms) : Negative regulation of MDR1 promoter activity in MCF-7, but not in multidrug resistant MCF-7/Adr, cells by cross coupled NF-kappa B/p65 and c-Fos transcription factors and their interaction with the CAAT region
Kaiser et al., Gastroenterology 1999 : TNF-alpha stimulated NF-kappaB activation and nuclear translocation and the degradation of Ikappa-Balpha were blocked by mesalamine
Gabriel et al., Brain Res Mol Brain Res 1999 (Arterial Occlusive Diseases...) : It is proposed that the highly inducible NF-kappaB complexes resulted from induction of p65 and activation of NF-kappaB in post-ischemic reactive glia
Bonizzi et al., Biochem Pharmacol 1999 : The PKC inhibitor calphostin C inhibited NF-kappaB activation by tumor necrosis factor (TNF)-alpha or interleukin (IL)-1beta in Jurkat or NIH3T3 cells but not in MCF7 A/Z cells ... The PKC inhibitor calphostin C inhibited NF-kappaB activation by tumor necrosis factor (TNF)-alpha or interleukin (IL)-1beta in Jurkat or NIH3T3 cells but not in MCF7 A/Z cells ... The PKC inhibitor calphostin C inhibited NF-kappaB activation by tumor necrosis factor (TNF)-alpha or interleukin (IL)-1beta in Jurkat or NIH3T3 cells but not in MCF7 A/Z cells
Turpin et al., J Biol Chem 1999 : IkappaBalpha controls the transcriptional activity of nuclear factor (NF)-kappaB by retaining it in the cytoplasm ; but, when expressed in the nucleus, it can also inhibit the interaction of NF-kappaB with DNA and promote the export of NF-kappaB from the nucleus to the cytoplasm
Lee et al., Hepatology 1999 (Necrosis) : Pretreatment with neutralizing anti-TNF antibodies, which inhibits hepatocyte DNA synthesis after PH, also reduces post-PH hepatic mitochondrial oxidant production by 80 % and inhibits NF kappaB activation and UCP-2 induction by 50 % and 80 %, respectively
Chen et al., Mol Pharmacol 1999 : SB 203580 but not PD 98059 inhibited the LPS induced stimulation of NF-kappaB DNA-protein binding
Rahman et al., Mol Pharmacol 1999 : We tested the hypothesis that activation of protein kinase C ( PKC ) and generation of oxidants are critical sequential signals mediating tumor necrosis factor (TNF)-alpha induced activation of nuclear factor-kappaB (NF-kappaB) and transcription of the intercellular adhesion molecule (ICAM)-1 gene ... However, both PKC activation and oxidant generation were necessary for ICAM-1 mRNA expression because the pretreatment of HPAE cells with either calphostin C or N-acetylcysteine inhibited the TNF-alpha induced activation of NF-kappaB and prevented the activation of ICAM-1 promoter
Shimada et al., Dig Dis Sci 1999 : TNF-alpha induced activation of NF-kappaB activity was suppressed by NAC , and H2O2 caused significant activation of NF-kappaB ... TNF-alpha induced activation of NF-kappaB activity was suppressed by NAC, and H2O2 caused significant activation of NF-kappaB
Garrett et al., Biochim Biophys Acta 1999 (Precursor B-Cell Lymphoblastic Leukemia-Lymphoma) : In CD14 transfected 70Z/3 pre-B lymphocyte tumor cells, these responses include activation of the MAP kinase homolog, p38 , activation of NF-kappaB , and transcription of kappa light chains, leading to the assembly of surface IgM ... SB203580 did not inhibit LPS induced NF-kappaB activation
Kupatt et al., Circ Res 1999 (Myocardial Ischemia...) : We studied the role of early tumor necrosis factor-alpha (TNF-alpha) cleavage and nuclear factor-kappaB (NF-kappaB) activation on ICAM-1 expression and venular adhesion of PMN in isolated hearts after ischemia ( 15 minutes ) and reperfusion ( 30 to 480 minutes ) ... Decoy NF-kappaB but not control oligonucleotides blocked ICAM-1 upregulation and inhibited the subacute increase in PMN adhesion ... These data suggest that ischemia and reperfusion in isolated hearts cause liberation of TNF-alpha , activation of NF-kappaB , and upregulation of ICAM-1, an adhesion molecule involved in inflammatory response after ischemia and reperfusion ... These data suggest that ischemia and reperfusion in isolated hearts cause liberation of TNF-alpha, activation of NF-kappaB , and upregulation of ICAM-1 , an adhesion molecule involved in inflammatory response after ischemia and reperfusion
Pahan et al., J Biol Chem 1999 : In contrast to the inability of LPS and IL-1beta alone to induce the expression of iNOS, both LPS and IL-1beta individually stimulated MAP kinase activity and induced DNA binding and transcriptional activity of NF-kappaB ... In contrast to the inability of LPS and IL-1beta alone to induce the expression of iNOS, both LPS and IL-1beta individually stimulated MAP kinase activity and induced DNA binding and transcriptional activity of NF-kappaB ... Moreover, wortmannin had no effect on LPS- or IL-1beta mediated activation of MAP kinase and NF-kappaB , suggesting that wortmannin induced the expression of iNOS in LPS- or IL-1beta stimulated C6 glial cells without modulating the activation of MAP kinase and NF-kappaB ... Similar to C6 glial cells, wortmannin also stimulated LPS mediated expression of iNOS and production of NO in astrocytes without affecting the LPS mediated activation of NF-kappaB
Sang et al., Arch Biochem Biophys 1999 (Shock, Septic) : The results show that LPS ( 4 mg/kg, ip ) induced the production of the cytokine genes and increased the nuclear protein level of NF-kappaB within 30 min after LPS administration
Lin et al., Immunity 1999 : Conversely, expression of kinase-deficient Cot inhibits CD3/CD28 but not TNF alpha induction of NF-kappaB
Verhasselt et al., J Immunol 1999 : We first found that NAC inhibited the constitutive as well as the LPS induced activity of the transcription factor NF-kappaB ... We first found that NAC inhibited the constitutive as well as the LPS induced activity of the transcription factor NF-kappaB
Hsing et al., J Immunol 1999 : Surprisingly, up-regulation of Fas, CD23, and ICAM-1 was partially independent, and up-regulation of LFA-1 was completely independent, of CD40 induced NF-kappaB activation ... Surprisingly, up-regulation of Fas , CD23, and ICAM-1 was partially independent, and up-regulation of LFA-1 was completely independent , of CD40 induced NF-kappaB activation ... Surprisingly, up-regulation of Fas, CD23 , and ICAM-1 was partially independent, and up-regulation of LFA-1 was completely independent , of CD40 induced NF-kappaB activation ... Surprisingly, up-regulation of Fas, CD23, and ICAM-1 was partially independent, and up-regulation of LFA-1 was completely independent , of CD40 induced NF-kappaB activation
Algarté et al., J Virol 1999 (Respirovirus Infections) : These experiments demonstrate a major role for IkappaBalpha in the regulation of NF-kappaB induced IFN-beta gene activation and a minor role for IkappaBbeta in the activation process ... These experiments demonstrate a major role for IkappaBalpha in the regulation of NF-kappaB induced IFN-beta gene activation and a minor role for IkappaBbeta in the activation process
Harcourt et al., J Virol 1999 (Hemorrhagic Fever, Ebola) : In contrast, infection with EZ did not block activation of the transcription factor NF-kappaB by IL-1beta
Manna et al., Biochem Pharmacol 1999 : TNF induced NF-kappaB activation, p65 translocation, IkappaBalpha degradation, and NF-kappaB dependent reporter gene expression were inhibited in cells pretreated with beta-lapachone
Zhang et al., J Biol Chem 1999 : LPS induced NF-kappaB activation was not inhibited by a dominant negative mutant of TRAF2 that is involved in TNF signaling
Ouaaz et al., J Exp Med 1999 : Our results indicate not only that RelA is required for activation of key genes involved in adaptive ( acquired ) immune responses, including major histocompatibility complex class I, CD40, and the Fas death receptor, but also that both NF-kappaB inducing signals and IFN-gamma are necessary for maximal activation
Lum et al., Biochem Pharmacol 1998 : CVT-634 ( 5-methoxy-1-indanone-3-acetyl-leu-D-leu-1-indanylamide ) prevented lipopolysaccharide (LPS), tumor necrosis factor (TNF)- , and phorbol ester induced activation of nuclear factor kappa B (NF-kappa B) in vitro by preventing signal induced degradation of I kappa B-alpha ... CVT-634 ( 5-methoxy-1-indanone-3-acetyl-leu-D-leu-1-indanylamide ) prevented lipopolysaccharide (LPS) , tumor necrosis factor (TNF)-, and phorbol ester induced activation of nuclear factor kappa B (NF-kappa B) in vitro by preventing signal induced degradation of I kappa B-alpha ... These studies suggest that specific inhibition of the chymotrypsin-like activity of the proteasome is sufficient to prevent signal induced NF-kappa B activation and that the proteasome is a novel target for the identification of agents that may be useful in the treatment of diseases whose etiology is dependent upon the activation of NF-kappa B
Jeon et al., Toxicol Lett 1999 : Treatment of AAF to RAW 264.7 cells induced a dose related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF ... Treatment of AAF to RAW 264.7 cells induced a dose related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while either AP-1 or NF-IL6 activation was not affected by AAF ... These data suggest that AAF inhibits iNOS gene expression by a mechanism involving a blockade of LPS induced nuclear translocation of NF-kappa B/Rel
Nick et al., J Clin Invest 1999 : Specific inhibitors of p38alpha MAPk blocked LPS induced adhesion, nuclear factor-kappa B (NF-kappaB) activation , and synthesis of tumor necrosis factor-alpha (TNF-alpha) ... Specific inhibitors of p38alpha MAPk blocked LPS induced adhesion, nuclear factor-kappa B (NF-kappaB) activation, and synthesis of tumor necrosis factor-alpha (TNF-alpha) ... Specific inhibitors of p38alpha MAPk blocked LPS induced adhesion, nuclear factor-kappa B (NF-kappaB) activation, and synthesis of tumor necrosis factor-alpha (TNF-alpha)
Goto et al., Cytokine 1999 : Involvement of NF-kappaB p50/p65 heterodimer in activation of the human pro-interleukin-1beta gene at two subregions of the upstream enhancer element
Soler et al., Eur J Cell Biol 1999 : Tumor necrosis factor-alpha (TNF) induces apoptosis in confluent LLC-PK1 epithelial cells, but also activates NF-kappaB , a negative regulator of apoptosis
Hinz et al., Mol Cell Biol 1999 : Moreover, inhibition of NF-kappaB caused a pronounced reduction of serum induced cyclin D1-associated kinase activity and resulted in delayed phosphorylation of the retinoblastoma protein ... Furthermore, NF-kappaB promotes G1-to-S-phase transition in mouse embryonal fibroblasts and in T47D mammary carcinoma cells
Zhao et al., J Biol Chem 1999 : We now show that MEKK2 and MEKK3 can in vivo activate IKK-alpha and IKK-beta, induce site-specific IkappaBalpha phosphorylation, and, relatively modestly, activate an NF-kappaB reporter gene ... We now show that MEKK2 and MEKK3 can in vivo activate IKK-alpha and IKK-beta, induce site-specific IkappaBalpha phosphorylation, and, relatively modestly, activate an NF-kappaB reporter gene ... We now show that MEKK2 and MEKK3 can in vivo activate IKK-alpha and IKK-beta, induce site-specific IkappaBalpha phosphorylation, and, relatively modestly, activate an NF-kappaB reporter gene ... In addition, dominant negative versions of either IKK-alpha or IKK-beta abolish NF-kappaB activation induced by MEKK2 or MEKK3, thereby providing evidence that these IKKs mediate the NF-kappaB inducing activities of these MEKKs ... In addition, dominant negative versions of either IKK-alpha or IKK-beta abolish NF-kappaB activation induced by MEKK2 or MEKK3 , thereby providing evidence that these IKKs mediate the NF-kappaB inducing activities of these MEKKs
Montaner et al., J Biol Chem 1999 : Here, we demonstrate that activation of NF-kappaB by RhoA does not exclusively promote its nuclear translocation and binding to the specific kappaB sequences ... NF-kappaB is also involved in the regulation of the transcriptional activity of the c-fos serum response factor (SRF) , since the activation of a SRE dependent promoter by RhoA can be efficiently interfered by the double mutant IkappaBalphaS32A/S36A, an inhibitor of the NF-kappaB activity
Tamatani et al., J Biol Chem 1999 : Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons ... Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons ... Tumor necrosis factor induces Bcl-2 and Bcl-x expression through NFkappaB activation in primary hippocampal neurons ... Expression of the mutant NFkappaB completely inhibited NFkappaB DNA binding activity and inhibited both TNF induced up-regulation of Bcl-2 and Bcl-x expression and neuroprotective effect ... Expression of the mutant NFkappaB completely inhibited NFkappaB DNA binding activity and inhibited both TNF induced up-regulation of Bcl-2 and Bcl-x expression and neuroprotective effect ... Expression of the mutant NFkappaB completely inhibited NFkappaB DNA binding activity and inhibited both TNF induced up-regulation of Bcl-2 and Bcl-x expression and neuroprotective effect
Rodriguez et al., J Biol Chem 1999 : Transcriptional activation of nuclear factor kappaB (NF-kappaB) is mediated by signal induced phosphorylation and degradation of its inhibitor, IkappaBalpha ... However, NF-kappaB activation induces rapid resynthesis of IkappaBalpha , which is responsible for post-induction repression of transcription
Wadsworth et al., Biochem Pharmacol 1999 : The effect of lipopolysaccharide (LPS) stimulated activation of NF-KB and the subsequent production of tumor necrosis factor alpha (TNF-alpha) and NO was determined in the macrophage cell line RAW 264.7 ... Our results do not support the view that wine antioxidants inhibit LPS induced NF-KB activation but instead that they have a more selective action on genes activated by LPS
Ponnappan et al., Cell Immunol 1999 : This failure to degrade IkappaBalpha may underlie both the observed decrease in NFkappaB induction and the IL-2 receptor expression in TNF treated T cells during aging
van Puijenbroek et al., Cytokine 1999 : Phospholipase A2 inhibitors and leukotriene synthesis inhibitors block TNF induced NF-kappaB activation ... Phospholipase A2 inhibitors and leukotriene synthesis inhibitors block TNF induced NF-kappaB activation ... The role, however, of AA and its metabolites in TNF induced NF-kappaB activation is still obscure
Rovin et al., Cytokine 1999 : Because NF-kappaB is necessary for MCP-1 gene expression, the effect of p38 kinase inhibition on IL-1beta induction of NF-kappaB was measured ... Because NF-kappaB is necessary for MCP-1 gene expression, the effect of p38 kinase inhibition on IL-1beta induction of NF-kappaB was measured ... Because NF-kappaB is necessary for MCP-1 gene expression, the effect of p38 kinase inhibition on IL-1beta induction of NF-kappaB was measured ... SB 203580 ( up to 25 microM ) had no effect on IL-1beta induced NF-kappaB nuclear translocation or DNA binding activity ... PD 098059, a selective inhibitor of the ERK activating kinase MEK1, had no effect on IL-1beta induced MCP-1 mRNA or protein levels, or on IL-1beta activation of NF-kappaB ... PD 098059, a selective inhibitor of the ERK activating kinase MEK1, had no effect on IL-1beta induced MCP-1 mRNA or protein levels, or on IL-1beta activation of NF-kappaB
Barcova et al., Pflugers Arch 1999 : Thus HIV-1 gp41 induced IL-10 up-regulation in monocytes may not involve NF-kappaB , MAPK, or PI 3-kinase activation, but rather may operate through activation of adenylate cyclase and pertussis-toxin-sensitive Gi/Go protein to effect p70 ( S6 ) -kinase activation
Lo et al., J Surg Res 1999 : LPS stimulated RAW cell TNF mRNA expression and NF kappa B activity
Chang et al., J Surg Res 1999 (Infection) : At 3 h postcecal incision, DMSO inhibited sepsis induced hepatic NF-kappa B activation and hepatic ICAM-1 gene expression to control levels and suppressed serum TNF by 75 %
Mori et al., Blood 1999 (Leukemia-Lymphoma, Adult T-Cell) : The viral protein Tax induces the activation and nuclear translocalization of transcription factor NF-kappaB , which is proposed to play a crucial role in the transformation of T cells by HTLV-I
Fraser et al., Eur J Immunol 1999 : CTLA4 ligation attenuates AP-1, NFAT and NF-kappaB activity in activated T cells
Dudley et al., Eur J Immunol 1999 : NF-kappaB regulates Fas/APO-1/CD95- and TCR- mediated apoptosis of T lymphocytes ... NF-kappaB regulates Fas/APO-1/CD95- and TCR- mediated apoptosis of T lymphocytes
Brand et al., Gastroenterology 1999 : Stimulation of ICAM expression is mediated by activation of the transcription factor NF-kappaB
Medvedev et al., J Biol Chem 1999 : Unlike lipopolysaccharide, C2-ceramide failed to activate NF-kappaB and did not induce production of tumor necrosis factor or interleukin-6 ... Unlike lipopolysaccharide, C2-ceramide failed to activate NF-kappaB and did not induce production of tumor necrosis factor or interleukin-6 ... The lipopolysaccharide antagonist, Rhodobacter sphae-roides diphosphoryl lipid A, inhibited lipopolysaccharide activation of NF-kappaB and AP-1 but did not block C2-ceramide induced AP-1
Papi et al., J Biol Chem 1999 (Asthma...) : Rhinovirus induction of ICAM-1 promoter activity was critically dependent upon up-regulation of NF-kappaB proteins binding to the -187/-178 NF-kappaB binding site on the ICAM-1 promoter
Iacobelli et al., J Immunol 1999 : The current study clearly demonstrates that IL-2 does not induce I kappa B alpha degradation or NF-kappa B activation in primary human T cells that respond to IL-2 by entering the cell cycle and avoiding apoptosis
Camenisch et al., J Immunol 1999 (Sepsis...) : The increase in TNF-alpha production appeared to be the result of a substantial increase in the LPS dependent activation of NF-kappa B nuclear translocation resulting in greater TNF-alpha production by macrophages from merkd mice
Ninomiya-Tsuji et al., Nature 1999 : Stimulation of TAK1 causes activation of NF-kappaB , which is blocked by dominant negative mutants of NIK, and an inactive TAK1 mutant prevents activation of NF-kappaB that is mediated by IL-1 but not by NIK
Taylor et al., Hepatology 1999 : Previously, we demonstrated that nuclear factor-kappaB (NF-kappaB) mediates cytokine induced hepatic inducible nitric oxide synthase (iNOS) expression
Devalaraja et al., Cancer Res 1999 (Melanoma) : We also show here that the resultant elevated nuclear NF-kappaB ( p50/p65 ) in these cells is responsible for the increased basal transcription of MGSA/GRO-alpha ... Cotransfection of a 350-bp MGSA/GRO-alpha promoter-luciferase reporter construct with either the dominant negative IKK-alpha or the repressors of NF-kappaB, the IkappaB-alpha wild type or mutants lacking the inducible phosphorylation sites, demonstrates that the increased basal MGSA/GRO-alpha transcription in the Hs294T cells is due to the enhanced nuclear activation of NF-kappaB
Liu et al., Mol Pharmacol 1999 (Shock, Septic) : Pretreatment of rats with pyrrolidine dithiocarbamate ( 50, 100, and 200 mg/kg, i.p. ), an inhibitor of NF-kappaB activation, prevented LPS induced I-kappaBalpha degradation and the resultant NF-kappaB activation and inhibited, in a dose related manner, the LPS induced CINC and ICAM-1 mRNA and protein expression
Kim et al., Mol Cells 1999 : Octamer motif is required for the NF-kappaB mediated induction of the inducible nitric oxide synthase gene expression in RAW 264.7 macrophages ... Electrophoretic mobility shift assay ( EMSA ) performed with the NF-kappaB site or the octamer motif containing oligonucleotide probe revealed that NF-kappaB binding was induced by LPS treatment, while the Oct-1 binding was constitutive
Laín de Lera et al., Oncogene 1999 : Unexpectedly, the presence of nuclear I(kappa)B(alpha) did not inhibit NF-kappaB binding to DNA and this phenomenon was not due to the presence of IkappaBbeta at the nuclear level
Yerneni et al., Diabetes 1999 (Hyperglycemia) : The augmented TNF-alpha induced NF-kappaB activation in HG was associated with increased TNF-alpha mediated transcriptional activation of the vascular cell adhesion molecule-1 promoter
Pan et al., J Biol Chem 1999 (Adenocarcinoma...) : We previously demonstrated that BK-stimulated NF-kappaB activation requires the small GTPase RhoA ... Tumor necrosis factor alpha (TNFalpha) also stimulated increased PI 3-kinase activity, however TNFalpha stimulated NF-kappaB activation was not affected by the PI 3-kinase inhibitors or the p85 dominant negative mutant ... Tumor necrosis factor alpha (TNFalpha) also stimulated increased PI 3-kinase activity, however TNFalpha stimulated NF-kappaB activation was not affected by the PI 3-kinase inhibitors or the p85 dominant negative mutant ... Tumor necrosis factor alpha (TNFalpha) also stimulated increased PI 3-kinase activity, however TNFalpha stimulated NF-kappaB activation was not affected by the PI 3-kinase inhibitors or the p85 dominant negative mutant
Koseki et al., J Biol Chem 1999 : Expression of CIPER induced NF-kappaB activation, which was inhibited by dominant negative NIK and a nonphosphorylable IkappaB-alpha mutant but not by dominant negative RIP ... Point mutations in highly conserved residues in the CARD of CIPER disrupted the ability of CIPER to activate NF-kappaB and to form homodimers, indicating that the CARD is essential for NF-kappaB activation and dimerization
Sakurai et al., J Biol Chem 1999 : Here we investigated the molecular mechanism of TAK1 induced NF-kappaB activation ... Dominant negative mutants of IkappaB kinase (IKK) alpha and IKKbeta inhibited TAK1 induced NF-kappaB activation ... Dominant negative mutants of IkappaB kinase (IKK) alpha and IKKbeta inhibited TAK1 induced NF-kappaB activation ... Dominant negative mutants of IkappaB kinase (IKK) alpha and IKKbeta inhibited TAK1 induced NF-kappaB activation ... Furthermore, tumor necrosis factor-alpha activated endogenous TAK1, and the kinase negative TAK1 acted as a dominant negative inhibitor against tumor necrosis factor-alpha induced NF-kappaB activation
Tsai et al., Br J Pharmacol 1999 : In electrophoretic mobility shift assays, the activation of NFkappaB induced by LPS for 1 h was inhibited by resveratrol ( 30 microM )
Erl et al., Circ Res 1999 : Transfer of IkappaBalpha reduced human IAP-1 mRNA levels, which suggested that IAP-1 is transcriptionally regulated by NF-kappaB
Han et al., Circ Res 1999 : Pretreatment with a potent inhibitor of IkappaBalpha proteolysis, TPCK, completely blocked Sar1 Ang IIAng II-induced NF-kappaB activation and induction of endogenous IL-6 gene expression, which indicated the essential role of NF-kappaB in mediating IL-6 expression
Kanakaraj et al., J Exp Med 1999 (Cytomegalovirus Infections) : Interleukin (IL)-18 is functionally similar to IL-12 in mediating T helper cell type 1 ( Th1 ) response and natural killer ( NK ) cell activity but is related to IL-1 in protein structure and signaling, including recruitment of IL-1 receptor associated kinase (IRAK) to the receptor and activation of c-Jun NH2-terminal kinase (JNK) and nuclear factor (NF)-kappaB
Maus et al., Antisense Nucleic Acid Drug Dev 1999 : These DOTMA/DOPE induced changes were not due to alterations in VCAM-1 mRNA stability, nor did DOTMA/DOPE inhibit TNF-alpha induced NF-kappaB-like binding activity in nuclear extracts of HPAEC, as analyzed by electrophoretic mobility shift assay
Takeda et al., Science 1999 (Limb Deformities, Congenital...) : Thus, IKKalpha is essential for NF-kappaB activation in the limb and skin during embryogenesis ... In contrast, there was no impairment of NF-kappaB activation induced by either interleukin-1 or tumor necrosis factor-alpha in IKKalpha-deficient embryonic fibroblasts and thymocytes, indicating that IKKalpha is not essential for cytokine induced activation of NF-kappaB ... In contrast, there was no impairment of NF-kappaB activation induced by either interleukin-1 or tumor necrosis factor-alpha in IKKalpha-deficient embryonic fibroblasts and thymocytes, indicating that IKKalpha is not essential for cytokine induced activation of NF-kappaB ... In contrast, there was no impairment of NF-kappaB activation induced by either interleukin-1 or tumor necrosis factor-alpha in IKKalpha-deficient embryonic fibroblasts and thymocytes, indicating that IKKalpha is not essential for cytokine induced activation of NF-kappaB
Li et al., Science 1999 : Mouse embryonic fibroblast cells that were isolated from IKK2-/- embryos showed a marked reduction in tumor necrosis factor-alpha (TNF-alpha)- and interleukin-1alpha induced NF-kappaB activity and an enhanced apoptosis in response to TNF-alpha ... Mouse embryonic fibroblast cells that were isolated from IKK2-/- embryos showed a marked reduction in tumor necrosis factor-alpha (TNF-alpha)- and interleukin-1alpha induced NF-kappaB activity and an enhanced apoptosis in response to TNF-alpha
Lee et al., J Virol 1999 (Cell Transformation, Viral) : Mutational analysis also revealed that STP-C488 induced NF-kappaB activation that was correlated with its ability to associate with TRAFs
Fiedler et al., J Virol 1999 : Incomplete regulation of NF-kappaB by IkappaBalpha during respiratory syncytial virus infection in A549 cells ... Respiratory syncytial virus ( RSV ) infection of airway epithelial cells results in persistent NF-kappaB activation and NF-kappaB mediated interleukin-8 production ... Previous studies in airway epithelial cells demonstrated that tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation is transient due to regulation by IkappaBalpha ... Studies presented here demonstrate that neither increased IkappaBalpha production which occurs as a result of RSV induced NF-kappaB activation nor inhibition of proteasome mediated IkappaBalpha degradation results in a reversal of RSV induced NF-kappaB activation ... Thus, while manipulation of IkappaBalpha results in reversal of TNF-alpha induced NF-kappaB activation, manipulation of IkappaBalpha does not result in a reversal of RSV induced NF-kappaB activation
Toyoshima et al., Biochem Biophys Res Commun 1999 (Carcinoma, Squamous Cell...) : These findings indicate that NO up-regulates ICAM-1 expression on cancer cells by a regulatory mechanism involving PKC and suggest that NF-kappaB , but not AP-1, might be involved in induction of ICAM-1 by NO in cancer cells
Fujita et al., Nihon rinsho. Japanese journal of clinical medicine 1999 (Diabetic Angiopathies...) : In macroangiopathy, AGE plays an important role in atherogesis through NF-kappa B activation, that induces VCAM-1 and MCP-1 ... In macroangiopathy, AGE plays an important role in atherogesis through NF-kappa B activation, that induces VCAM-1 and MCP-1
Isaacs et al., J Cell Physiol 1999 : Role of NFkappaB in the regulation of macrophage colony stimulating factor by tumor necrosis factor-alpha in ST2 bone stromal cells ... NaS decreased TNFalpha stimulated NFkappaB activation by 50 % as assessed by EMSA
Kalogeris et al., Am J Physiol 1999 : These effects of lactacystin and PS-341 were associated with inhibitory effects on TNF stimulated NF-kappaB activation in both HUVEC and ECV ... Our results demonstrate the importance of the 26S proteasome in TNF induced activation of NF-kappaB , ECAM expression, and leukocyte-endothelial adhesive interactions in vitro
Payne et al., Cell Death Differ 1998 : The pre-treatment of cells with specific inhibitors of these proteins [ pyrrolidine dithiocarbamate ( NF-kappaB inhibitor ) and 3-aminobenzamide ( PARP inhibitor ) ] sensitizes cells to the induction of apoptosis by NaDOC, indicating that these stress response pathways are protective in nature
Hoshino et al., J Immunol 1999 : Overexpression of wild-type TLR4 but not the mutant TLR4 from C3H/HeJ mice activated NF-kappaB
Kuprash et al., J Immunol 1999 : Our observations support the notion that full LPS response of TNF gene requires both NF-kappaB and non-NF-kappaB nuclear proteins ... Our observations support the notion that full LPS response of TNF gene requires both NF-kappaB and non-NF-kappaB nuclear proteins
Kouba et al., J Immunol 1999 : These data suggest that in fibroblasts TNF-alpha activates and initiates the nuclear translocation of NF-kappa B that binds a divergent NF-kappa B element and plays a critical role in the observed inhibition of alpha 2 ( I ) collagen gene transcription
Jobin et al., J Immunol 1999 (Cell Transformation, Viral) : We characterized the effect of a dominant negative TRAF-2 delivered by an adenoviral vector ( Ad5dnTRAF-2 ) on the cytokine signaling cascade in several IEC and also investigated whether inhibiting the TRAF-2 transmitting signal blocked TNF-alpha induced NF-kappa B and IL-8 gene expression ... Ad5dnTRAF-2 expression prevented TNF-alpha induced, but not IL-1 beta induced, I kappa B alpha degradation and NF-kappa B activation in NIH-3T3 and IEC-6 cells
Wang et al., Nat Med 1999 (Neoplasms, Experimental) : We show here that inhibition of NF-kappaB through the adenoviral delivery of a modified form of IkappaBalpha , the inhibitor of NF-kappaB , sensitizes chemoresistant tumors to the apoptotic potential of TNFalpha and of the chemotherapeutic compound CPT-11, resulting in tumor regression
Nakashima et al., J Am Soc Nephrol 1999 : Cells were stimulated with TNF-alpha or LPS plus IFN-gamma in the presence of NF-kappaB inhibitors, herbimycin A (HerA), or the more specific JAK2 inhibitor AG490 ... Cells were stimulated with TNF-alpha or LPS plus IFN-gamma in the presence of NF-kappaB inhibitors, herbimycin A (HerA), or the more specific JAK2 inhibitor AG490 ... p65 was inhibited by the three NF-kappaB inhibitors described above, whereas p50 was not
Ishizuka et al., Eur J Pharmacol 1999 : These findings suggest that the synergistic enhancement of VCAM-1 expression by TNF-alpha and endothelin ET ( B ) receptor stimulation may be augmented by the induction of NF-kappaB binding activity in human vascular endothelial cells
Page et al., J Biol Chem 1999 : Extensively oxidized low density lipoprotein ( ox-LDL ), a modulator of atherogenesis, down-regulates the lipopolysaccharide (LPS) induced activation of transcription factor NF-kappaB
Swantek et al., J Biol Chem 1999 : Transcriptional regulation of the TNF-alpha gene is dependent on nuclear factor-kappaB (NF-kappaB)
Webster et al., Mol Cell Biol 1999 : Here we show that both p53 and NF-kappaB inhibit each other 's ability to stimulate gene expression and that this process is controlled by the relative levels of each transcription factor ... Moreover, endogenous, tumor necrosis factor alpha activated NF-kappaB will inhibit endogenous wild-type p53 transactivation
Macián et al., Mol Cell Biol 1999 : Moreover, NFAT1 binds to the kappaB sites on the viral LTR and negatively regulates NF-kappaB mediated activation of HIV-1 transcription , by competing with NF-kappaB1 for its binding sites on the HIV-1 LTR
Kranzhöfer et al., Biochem Biophys Res Commun 1999 (Arteriosclerosis...) : This study therefore examined the effect of angiotensin (ANG) II on NF-kappaB activation in monocytic cells, a major cellular component of human atheroma, by electrophoretic mobility shift assay ... This study therefore examined the effect of angiotensin (ANG) II on NF-kappaB activation in monocytic cells, a major cellular component of human atheroma, by electrophoretic mobility shift assay ... ANG II , like TNFalpha, caused rapid activation of NF-kappaB in human mononuclear cells isolated from peripheral blood by Ficoll density gradient ... Moreover, ANG II stimulated NF-kappaB activation in human monocytes, but not in lymphocytes from the same preparation ... Moreover, ANG II stimulated NF-kappaB activation in human monocytes, but not in lymphocytes from the same preparation
Kayal et al., Mol Microbiol 1999 : By infecting in vitro human umbilical vein endothelial cells ( HUVEC ) with L. monocytogenes, we found that wild-type bacteria induced the expression of the adhesion molecules ( ICAM-1 and E-selectin ), chemokine secretion ( IL-8 and monocyte chemotactic protein-1 ) and NF-kappa B nuclear translocation ... By infecting in vitro human umbilical vein endothelial cells ( HUVEC ) with L. monocytogenes, we found that wild-type bacteria induced the expression of the adhesion molecules ( ICAM-1 and E-selectin ), chemokine secretion ( IL-8 and monocyte chemotactic protein-1 ) and NF-kappa B nuclear translocation
Chen et al., Life Sci 1999 : Several reports have documented that the activation of NF-kappaB triggers the expression of COX-2 gene ... In the present study, NF-kappaB was activated by TNF-alpha in rat aortic smooth muscle cells as demonstrated by electrophoretic mobility shift assay ... The activity of NF-kappaB induced by TNF-alpha was blocked by calpain inhibitor I, a potent NF-kappaB inhibitor ... The activity of NF-kappaB induced by TNF-alpha was blocked by calpain inhibitor I, a potent NF-kappaB inhibitor ... These results suggest that the transcriptional regulation of COX-2 expression by NF-kappaB depends upon the types of cells studied and that activation of this transcription factor alone does not play an important role in the expression of COX-2 in rat smooth muscle cells
Shames et al., J Surg Res 1999 : LPS Induced NF-kappaB activation and TNF-alpha release in human monocytes are protein tyrosine kinase dependent and protein kinase C independent ... LPS Induced NF-kappaB activation and TNF-alpha release in human monocytes are protein tyrosine kinase dependent and protein kinase C independent ... Previous studies have implicated activation of both protein kinase C ( PKC ) and protein tyrosine kinases ( PTK ) in LPS induced NF-kappaB activation and TNF-alpha production ... We hypothesized that inhibition of either PKC or PTK would decrease LPS induced NF-kappaB DNA binding and TNF-alpha release in human monocytes ... LPS increased NF-kappaB DNA binding and TNF-alpha release in human monocytes ... Nonspecific protein kinase inhibition inhibited NF-kappaB activation and TNF-alpha release, while specific PKC inhibition with Bis had no effect on LPS induced NF-kappaB DNA binding or TNF-alpha release ... PTK inhibition with Gen attenuated both LPS induced NF-kappaB DNA binding and TNF-alpha production in human monocytes ... PTK inhibition with Gen attenuated both LPS induced NF-kappaB DNA binding and TNF-alpha production in human monocytes ... These results suggest that LPS induced NF-kappaB activation and TNF-alpha release in human monocytes are independent of PKC activity ... Furthermore, our results provide evidence that PTK plays a role in LPS induced NF-kappaB activation and TNF-alpha release in human monocytes and thus could be a potential therapeutic target in inflammatory states ... Furthermore, our results provide evidence that PTK plays a role in LPS induced NF-kappaB activation and TNF-alpha release in human monocytes and thus could be a potential therapeutic target in inflammatory states
Makkonen et al., Eur J Pharm Sci 1999 (Arthritis, Rheumatoid) : Both clodronate and its metabolite also inhibited the LPS stimulated binding of NF-kappaB to DNA
Alaaeddine et al., Arthritis Rheum 1999 (Osteoarthritis) : IL-4 had no effect on TNFalpha up-regulation of NF-kappaB , and a slight decrease was noted with IL-10 and IL-13 at the highest concentration used ( 5 ng/ml )
Eickelberg et al., J Biol Chem 1999 : In contrast, neither CCAAT enhancer binding protein-beta, NF-kappaB , nor Sp1 were activated by TGF-beta1
Doman et al., J Interferon Cytokine Res 1999 : TNF rapidly stimulated both NF-kappaB and JNK activation in MCF-7 and MCF-7/3E9 cells, but JNK activation was significantly reduced ( threefold ) in apoptotically resistant cells
Zheng et al., J Pharmacol Exp Ther 1999 : In RA tissue, but not in the CM, inhibitors of NF-kappaB activation blocked the appearance of both functional iNOS and iNOS mRNA
Browder et al., J Trauma 1999 (Sepsis) : CLP induced pulmonary NFkappaB activation at 3, 4, and 8 hours ( p < 0.05 )
Bayón et al., Br J Pharmacol 1999 : 6. These data indicate that the incorporation of a 4-trifluoromethyl group to the salicylate molecule strongly enhances its inhibitory effect on NF-kappaB activation, VCAM-1 mRNA expression and iNOS induction , irrespective of the presence of the acetyl moiety involved in the inhibition of cyclo-oxygenase
Katsuyama et al., Endocrinology 1999 : Both IL-1beta and TNF-alpha activated nuclear factor (NF)-kappaB as demonstrated by electrophoretic mobility shift assay ... Both IL-1beta and TNF-alpha activated nuclear factor (NF)-kappaB as demonstrated by electrophoretic mobility shift assay ... IL-1beta caused a rapid increase in phosphorylated IkappaB-alpha levels and subsequent transient decrease in IkappaB-alpha levels, an inhibitor of NF-kappaB , as revealed by Western blot analysis using specific antibodies for phosphorylated and nonphosphorylated IkappaB-alpha ... The present study demonstrates that 1 ) inhibitory effect of dexamethasone on cytokine induced iNOS expression and NO production in rat VSMCs, although potentially acting at multiple levels, is partly mediated by inhibition of NF-kappaB activation resulting from decreased phosphorylation and degradation of IkappaB-alpha, 2 ) both salicylate and aspirin inhibit cytokine stimulated NO production at translational and/or posttranslational levels without affecting NF-kappaB- mediated iNOS gene expression, and 3 ) aspirin directly inhibits iNOS enzyme activity
Wahlstrom et al., Shock 1999 : Nuclear translocation of NF-kappaB is regulated by the cytoplasmic inhibitory factor IkappaBalpha
Hsuan et al., Microb Pathog 1999 : The role of Ca2+ and tyrosine kinases in NF-kappaB activation and inflammatory cytokine gene expression was studied, since an inhibitor of tyrosine kinases attenuates LPS induced [Ca2+ ] i elevation in BAMs
Selzman et al., Circ Res 1999 : Under these conditions, TNF-alpha induced NF-kappaB nuclear translocation and DNA binding were inhibited, NF-kappaB dependent luciferase activity was reduced by 50 %, there was no degradation of native IkappaBalpha detected, interleukin-6 production was reduced by 54 %, and VSMC proliferation was decreased by 60 %
Bertin et al., J Biol Chem 1999 : CARD4 interacted with the serine-threonine kinase RICK and potently induced NF-kappaB activity through TRAF-6 and NIK signaling molecules
Vig et al., J Biol Chem 1999 : Modulation of tumor necrosis factor and interleukin-1 dependent NF-kappaB activity by mPLK/IRAK ... However, it is currently unknown whether mPLK/IRAK catalytic activity is required for IL-1 dependent NF-kappaB activation ... However, it is currently unknown whether mPLK/IRAK catalytic activity is required for IL-1 dependent NF-kappaB activation ... Intriguingly, catalytically inactive mPLK/IRAK inhibits type 1 tumor necrosis factor (TNF) receptor dependent NF-kappaB activation
Qiu et al., J Biol Chem 1999 : In 16HBE human bronchial epithelial cells, IL-8 expression is regulated predominantly by NF-kappaB , and PG490 but not cyclosporin A can completely inhibit expression of IL-8
Lee et al., J Biol Chem 1999 (Neoplasms) : PG490 potently inhibited TNF-alpha induced activation of NF-kappaB ... Our identification of a compound that blocks TNF-alpha induced activation of NF-kappaB may enhance the cytotoxicity of TNF-alpha on tumors in vivo and limit its proinflammatory effects
Fischer et al., Virus Res 1999 (Cell Transformation, Viral...) : The results suggested that whereas differences in the activation of NF-kappaB through the various LMP1 isolates correlated tightly with their different expression levels, the outgrowth of transfected cells in soft agar did not and the transcription factor NF-kappaB therefore appeared not to be the major effector for the transformation of the rodent cell line ratl by LMP1
Janssen-Heininger et al., Am J Respir Cell Mol Biol 1999 : In contrast, TNF activated NF-kappaB in a Ras independent fashion ... Evaluation of members of the mitogen activated protein kinase ( MAPK ) family as downstream effectors of Ras revealed the requirement of MAPK/ extracellular regulated kinase (ERK) kinase kinase ( MEKK)1 and c-Jun N-terminal kinases in the induction of NF-kappaB by both oxidants and TNF , whereas the MEK-ERK pathway negatively regulates NF-kappaB
Kojima et al., J Immunol 1999 : An essential role for NF-kappa B in IL-18 induced IFN-gamma expression in KG-1 cells ... In addition, stable transformants of a dominant negative form of the I kappa B alpha showed an inhibition of IL-18 dependent I kappa B alpha degradation, NF-kappa B activation , and expression of IFN-gamma ... In addition, stable transformants of a dominant negative form of the I kappa B alpha showed an inhibition of IL-18 dependent I kappa B alpha degradation, NF-kappa B activation , and expression of IFN-gamma
Awane et al., J Immunol 1999 : IL-17 induced NF-kappa B protein-DNA complexes consisting of p65/p50 heterodimers in the rat intestinal epithelial cell line IEC-6 ... In addition to activation of the NF-kappa B , IL-17 regulated the activities of extracellular regulated kinase, c-Jun N-terminal kinase, and p38 mitogen activated protein kinases in IEC-6 cells ... In addition to activation of the NF-kappa B , IL-17 regulated the activities of extracellular regulated kinase, c-Jun N-terminal kinase, and p38 mitogen activated protein kinases in IEC-6 cells
Kieser et al., EMBO J 1999 (Cell Transformation, Neoplastic) : Whereas NF-kappaB activation by LMP1 was blocked by a dominant negative TRADD mutant, LMP1 induces JNK1 independently of the TRADD death domain and TRAF2, which binds to TRADD ... Whereas NF-kappaB activation by LMP1 was blocked by a dominant negative TRADD mutant, LMP1 induces JNK1 independently of the TRADD death domain and TRAF2, which binds to TRADD
Han et al., Eur J Immunol 1999 : DEX or the PAF antagonist BN 50739 significantly inhibited LPS induced NF-kappaB activation
Byrd et al., J Immunol 1999 : Fibroblast growth factor-1 (FGF-1) enhances IL-2 production and nuclear translocation of NF-kappaB in FGF receptor bearing Jurkat T cells
Takeuchi et al., Gene 1999 : Like other TLR family members, constitutively active TLR6 activates both NF-kappaB and c-Jun N-terminal kinase (JNK)
Díaz-Cazorla et al., J Am Soc Nephrol 1999 : Treatment with N ( G ) -nitro-L-arginine methyl ester ( L-NAME ) did not affect initial activation of NF-kappaB by IL-1beta/TNF-alpha , but unveiled an inhibitory effect of NO generation on NF-kappaB activity after 4 h ... Treatment with N ( G ) -nitro-L-arginine methyl ester ( L-NAME ) did not affect initial activation of NF-kappaB by IL-1beta/TNF-alpha , but unveiled an inhibitory effect of NO generation on NF-kappaB activity after 4 h ... These changes in IkappaBalpha expression could contribute to the dual effects of NO donors on NF-kappaB activation and COX-2 expression in HMC
Hsu et al., Blood 1999 : NF-kappaB activation is required for C5a induced interleukin-8 gene expression in mononuclear cells ... These results suggest that NF-kappaB activation is required for C5a induced IL-8 gene expression and that this response is mediated primarily through a pertussis toxin-insensitive pathway
Pyatt et al., Blood 1999 : An essential role for NF-kappaB in human CD34 ( + ) bone marrow cell survival ... However, a role for NF-kappaB in human CD34 ( + ) bone marrow cells has not been described
Norris et al., J Biol Chem 1999 : These experiments show that Ras utilizes Raf dependent and Raf independent pathways to activate NF-kappaB transcriptional activity, both of which require the stress activated kinase p38 or a related kinase
Liu et al., J Biol Chem 1999 : Pretreating Mo7e cells with SN50 blocked TNF-alpha induced nuclear translocation of NF-kappaB and inhibited TNF-alpha induced Mo7e cell survival and proliferation
Olsson et al., J Biol Chem 1999 : CTLA-4 ligation suppresses CD28 induced NF-kappaB and AP-1 activity in mouse T cell blasts ... CTLA-4 ligation suppresses CD28 induced NF-kappaB and AP-1 activity in mouse T cell blasts
Bondeson et al., Proc Natl Acad Sci U S A 1999 (Arthritis, Rheumatoid...) : We found that the spontaneous production of tumor necrosis factor alpha and other pro-inflammatory cytokines is NF-kappaB dependent in rheumatoid synovial tissue, in contrast to the main anti-inflammatory mediators, like IL-10 and -11, and the IL-1 receptor antagonist ... We found that the spontaneous production of tumor necrosis factor alpha and other pro-inflammatory cytokines is NF-kappaB dependent in rheumatoid synovial tissue, in contrast to the main anti-inflammatory mediators, like IL-10 and -11 , and the IL-1 receptor antagonist
Zhang et al., Nat Genet 1999 (Lymphoma, B-Cell, Marginal Zone...) : Wild-type BCL10 activated NF-kappaB but induced apoptosis of MCF7 and 293 cells
Wang et al., J Cell Biochem 1999 : NFkappaB activation by ECM was necessary for cellular differentiation, rather than inhibition, of apoptosis
Kramer et al., J Surg Res 1999 (Hemorrhage...) : LPS stimulation of sham Mphi increased NF-kappaB activation with corresponding loss of its inhibitor IkappaB-alpha ... LPS stimulation of sham Mphi increased NF-kappaB activation with corresponding loss of its inhibitor IkappaB-alpha
Bash et al., EMBO J 1999 : Rel/NF-kappaB can trigger the Notch signaling pathway by inducing the expression of Jagged1 , a ligand for Notch receptors ... Importantly, jagged1 transcripts were also upregulated by endogenous NF-kappaB activation and this effect was inhibited by a dominant mutant of IkappaBalpha , a physiological inhibitor of NF-kappaB ... Consistent with its Rel/NF-kappaB dependent induction , Jagged1 was found to be highly expressed in splenic B cells where c-Rel is expressed constitutively
Inohara et al., J Biol Chem 1999 : Unlike Apaf-1, Nod1 induced activation of nuclear factor-kappa-B (NF-kappaB) and bound RICK, a CARD containing kinase that also induces NF-kappaB activation ... Nod1 mutants inhibited NF-kappaB activity induced by RICK, but not that resulting from tumor necrosis factor-alpha stimulation
Zhang et al., J Biol Chem 1999 : In vitro studies demonstrated that TNF-alpha induced endothelial cell syndecan-4 expression by both increasing syndecan-4 gene expression in an NF-kappaB dependent manner and by prolonging syndecan-4 mRNA half-life
Mohan et al., Am J Physiol 1999 : Here we investigate the hypothesis that low shear induced activation of NF-kappaB is responsible for enhanced expression of vascular cell adhesion molecule ( VCAM-1 ) resulting in augmented endothelial cell-monocyte ( EC-Mn ) adhesion and that this activation is dependent on intracellular oxidant activity ... Here we investigate the hypothesis that low shear induced activation of NF-kappaB is responsible for enhanced expression of vascular cell adhesion molecule ( VCAM-1 ) resulting in augmented endothelial cell-monocyte ( EC-Mn ) adhesion and that this activation is dependent on intracellular oxidant activity ... However, cytokine induced NF-kappaB activation and VCAM-1 expression are blocked by both PDTC and NAC ... These data suggest that NF-kappaB plays a key role in low shear induced VCAM-1 expression and that pathways mediating low shear- and cytokine induced EC-Mn adhesion may be differentially regulated
Marumo et al., Diabetes 1999 : Using electrophoretic gel mobility shift assay, we observed that VEGF stimulated binding activity of NF-kappaB ... VEGF induced NF-kappaB activation was inhibited by TLCK and NAC , but not by PD 98059 ... VEGF induced NF-kappaB activation was inhibited by TLCK and NAC, but not by PD 98059
Li et al., Biochem Biophys Res Commun 1999 : Treatment of cells with gamma-tocopherol attenuated ox-LDL mediated degradation of IkappaB and activation of NF-kappaB ( both P < 0.01 vs ox-LDL alone )
Uehara et al., J Biol Chem 1999 (Glioma) : These results indicate that the transient translocation of NF-kappaB in response to IL-1beta may be partly dependent on transient proteasome activation ... The activation of NF-kappaB and the subsequent cytokine induced neutrophil chemoattractant induction in response to interleukin-1beta (IL-1beta) were inhibited by proteasome inhibitors, MG132 and proteasome inhibitor I. Translocation of NF-kappaB into nuclei occurs by the phosphorylation, multi-ubiquitination, and degradation of IkappaBalpha , a regulatory protein of NF-kappaB ... The activation of NF-kappaB and the subsequent cytokine induced neutrophil chemoattractant induction in response to interleukin-1beta (IL-1beta) were inhibited by proteasome inhibitors, MG132 and proteasome inhibitor I. Translocation of NF-kappaB into nuclei occurs by the phosphorylation, multi-ubiquitination, and degradation of IkappaBalpha, a regulatory protein of NF-kappaB
Feng et al., Infect Immun 1999 : The high-level expression of TNF-alpha persisted over 2 h. Dexamethasone blocked the activation of both NF-kappaB and AP-1 and suppressed the production of TNF-alpha in TEP macrophages stimulated by M. penetrans lipid extract of PK-digested LAMPs
Yu et al., Curr Biol 1999 : Overexpression of a dominant negative mutant of RIP3 strongly inhibited the caspase activation but not the NFkappaB activation induced by TNFalpha
Mechtcheriakova et al., Blood 1999 (Inflammation) : In contrast, NFkappaB , the major transcription factor involved in tissue factor upregulation by inflammatory stimuli, is not activated by VEGF
Kemler et al., Glia 1999 (Brain Neoplasms...) : Role of IkappaBalpha and IkappaBbeta in the biphasic nuclear translocation of NF-kappaB in TNFalpha stimulated astrocytes and in neuroblastoma cells ... Role of IkappaBalpha and IkappaBbeta in the biphasic nuclear translocation of NF-kappaB in TNFalpha stimulated astrocytes and in neuroblastoma cells
Bodine et al., Endocrinology 1999 (Bone Resorption) : Treatment of the cells with 17beta-E2 partially suppressed the activation of NF-kappaB by TNF alpha , but did not block cytokine induced IL-6 secretion
Joubert-Caron et al., Electrophoresis 1999 : The model used in this study was a human lymphoblastoid cell line in which a functional repression of the transcription factors NFkappaB was obtained by induction of overexpression of IkappaBalpha , a physiological inhibitor of NFkappaB
Li et al., Genes Dev 1999 (Skin Abnormalities) : NF-kappaB activation mediated by TNFalpha and IL-1 is diminished in IKK1-deficient mouse embryonic fibroblast (MEF) cells ... NF-kappaB activation mediated by TNFalpha and IL-1 is diminished in IKK1-deficient mouse embryonic fibroblast (MEF) cells ... Our results support a role for IKK1 in NF-kappaB activation and uncover its involvement in skin and skeleton development
Thomassen et al., Cytokine 1999 : It failed to bind to the known IL-1-family members and, when co-expressed with the IL-1RI, had no effect on the binding of IL-1 and on subsequent nuclear factor kappaB (NFkappaB) activation
Eliopoulos et al., J Biol Chem 1999 : We have found that inhibition of p38 by the highly specific inhibitor SB203580 did not affect NF-kappaB binding activity ... Divergence of signals must, however, occur downstream of TRAF2 as a dominant negative TRAF2 mutant that blocks LMP1 induced NF-kappaB activation also inhibited p38 signaling
Kirch et al., Oncogene 1999 : Expression of human p53 requires synergistic activation of transcription from the p53 promoter by AP-1, NF-kappaB and Myc/Max
Masamune et al., Gastroenterology 1999 (Helicobacter Infections...) : This effect was mimicked by sphingomyelinase, but not by phospholipase A2 or phospholipase C. C2-ceramide induced IL-8 gene transcription mainly through activation of NF-kappaB because mutation of the NF-kappaB binding site completely abrogated the induction of luciferase activity
Cowdery et al., J Immunol 1999 : Although LPS alone is a poor inducer of p40 transcription, both LPS and CpG DNA induce similar nuclear translocation of NF-kappaB ... Although LPS alone is a poor inducer of p40 transcription, both LPS and CpG DNA induce similar nuclear translocation of NF-kappaB
Díaz-Guerra et al., J Immunol 1999 : Treatment of cultured peritoneal macrophages with IFN-gamma resulted in tyrosine phosphorylation of IkappaBalpha and IkappaBbeta, NF-kappaB activation , and expression of inducible NO synthase (iNOS) ... Treatment of cultured peritoneal macrophages with IFN-gamma resulted in tyrosine phosphorylation of IkappaBalpha and IkappaBbeta , NF-kappaB activation , and expression of inducible NO synthase (iNOS) ... Treatment of cultured peritoneal macrophages with IFN-gamma resulted in tyrosine phosphorylation of IkappaBalpha and IkappaBbeta, NF-kappaB activation , and expression of inducible NO synthase (iNOS) ... Since tyrosine phosphorylation of IkappaBalpha is sufficient to activate NF-kappaB in Jurkat cells, macrophages were treated with the protein tyrosine phosphatase inhibitor peroxovanadate ( POV ), which elicited an intense tyrosine phosphorylation of both IkappaB ... Treatment with POV of macrophages stimulated with IFN-gamma or LPS potentiated the degradation of IkappaBalpha and IkappaBbeta, the activation of NF-kappaB , and the expression of iNOS
Vittimberga et al., J Surg Res 1999 : Transcriptional activation of the TNF-alpha gene in LPS stimulated macrophages is dependent upon nuclear factor kappa-B (NF-kappaB) activity
Mortellaro et al., J Immunol 1999 : New immunosuppressive drug PNU156804 blocks IL-2 dependent proliferation and NF-kappa B and AP-1 activation ... PNU156804 inhibition of NF-kappa B activation is due to the inhibition of the degradation of I kappa B-alpha and I kappa B-beta ... PNU156804 inhibition of NF-kappa B activation is due to the inhibition of the degradation of I kappa B-alpha and I kappa B-beta
Li et al., J Exp Med 1999 : Accordingly, IKKbeta-deficient cells are defective in activation of IKK and NF-kappaB in response to either tumor necrosis factor alpha or interleukin 1 ... Thus IKKbeta, but not IKKalpha, plays the major role in IKK activation and induction of NF-kappaB activity ... Thus IKKbeta, but not IKKalpha , plays the major role in IKK activation and induction of NF-kappaB activity ... Thus IKKbeta , but not IKKalpha, plays the major role in IKK activation and induction of NF-kappaB activity
Richard et al., Blood 1999 : Interleukin-9 regulates NF-kappaB activity through BCL3 gene induction ... This contrasts with tumor necrosis factor (TNF) dependent NF-kappaB activation, which occurs earlier, involves p65/p50 dimers, and is dependent on IkappaB degradation
Wang et al., J Am Soc Nephrol 1999 : Activation of NFkappaB by delipidated bovine serum albumin ( 15 mg/ml ) was detectable within 2 h, maximal after 8 h, and maintained for at least 16 h of continuous exposure ... Therefore, albumin increased NFkappaB and reduced IkappaB levels in PTC, and MCP-1 expression was dependent on NFkappaB activation ... Therefore, albumin increased NFkappaB and reduced IkappaB levels in PTC, and MCP-1 expression was dependent on NFkappaB activation
Takizawa et al., Br J Cancer 1999 : Moreover, a NF-kappaB nuclear translocation inhibitor did not inhibit the induction of ICAM-1 expression by treatment with CDDP and 5-FU
Jin et al., J Biol Chem 1999 : Mechanisms by which the human T-cell leukemia virus type I Tax oncoprotein activates NF-kappaB remain incompletely understood ... This direct interaction with IKK-gamma correlates with Tax induced IkappaB-alpha phosphorylation and NF-kappaB activation
Schwandner et al., J Biol Chem 1999 : Like LPS, sPGN and LTA bind to the glycosylphosphatidylinositol anchored membrane protein CD14 and induce activation of the transcription factor NF-kappaB in host cells like macrophages ... We show that whole Gram positive bacteria, sPGN and LTA induce the activation of NF-kappaB in HEK293 cells expressing TLR2 but not in cells expressing TLR1 or TLR4 ... The sPGN- and LTA induced NF-kappaB activation was not inhibited by polymyxin B, an antibiotic that binds and neutralizes LPS
Kaliman et al., J Biol Chem 1999 : Here we show that IGF-II induces nuclear factor-kappaB (NF-kappaB) and nitric-oxide synthase (NOS) activities downstream from PI 3-kinase and that these events are critical for myogenesis ... IGF-II induced iNOS expression and NO production were blocked by NF-kappaB inhibition ... Regarding the mechanisms involved in IGF-II activation of NF-kappaB , PI 3-kinase inhibition prevented NF-kappaB activation, iNOS expression, and NO production ... Regarding the mechanisms involved in IGF-II activation of NF-kappaB, PI 3-kinase inhibition prevented NF-kappaB activation, iNOS expression, and NO production
Srinivasula et al., J Biol Chem 1999 : Expression of the CARD domain of human CLAP abrogates tumor necrosis factor-alpha induced NF-kappaB activation, suggesting that cellular CLAP plays an essential role in this pathway of NF-kappaB activation
Haas et al., J Biol Chem 1999 : These cells show a permanent activation of the transcription factor NF-kappaB , exert constitutive p38 mitogen activated protein kinase activity, and produce high amounts of interleukin-6 ... These cells show a permanent activation of the transcription factor NF-kappaB , exert constitutive p38 mitogen activated protein kinase activity, and produce high amounts of interleukin-6
Bork et al., Planta Med 1999 : Here we show that micromolar concentrations of hypericin inhibited the PMA- and TNF-alpha induced activation of NF-kappa B in HeLa and TC10 cells, respectively
Seppänen et al., Oncol Res 1998 (Adenocarcinoma...) : In the present study, we have investigated the effects of interferons-alpha (IFN-alpha) and -gamma ( IFN-gamma ), interleukin-10 (IL-10) and -13 ( IL-13 ), transforming growth factor-beta1 ( TGF-beta1 ), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and tumor necrosis factor-alpha (TNF-alpha) on cell proliferation and induction of transcription factors AP-1 and NF-kappaB in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian carcinoma cells in vitro ... In the present study, we have investigated the effects of interferons-alpha (IFN-alpha) and -gamma ( IFN-gamma ), interleukin-10 (IL-10) and -13 ( IL-13 ), transforming growth factor-beta1 ( TGF-beta1 ), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and tumor necrosis factor-alpha (TNF-alpha) on cell proliferation and induction of transcription factors AP-1 and NF-kappaB in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian carcinoma cells in vitro ... In the present study, we have investigated the effects of interferons-alpha (IFN-alpha) and -gamma ( IFN-gamma ), interleukin-10 (IL-10) and -13 ( IL-13 ), transforming growth factor-beta1 ( TGF-beta1 ), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and tumor necrosis factor-alpha (TNF-alpha) on cell proliferation and induction of transcription factors AP-1 and NF-kappaB in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian carcinoma cells in vitro ... In the present study, we have investigated the effects of interferons-alpha (IFN-alpha) and -gamma ( IFN-gamma ), interleukin-10 (IL-10) and -13 ( IL-13 ), transforming growth factor-beta1 ( TGF-beta1 ), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and tumor necrosis factor-alpha (TNF-alpha) on cell proliferation and induction of transcription factors AP-1 and NF-kappaB in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian carcinoma cells in vitro ... In the present study, we have investigated the effects of interferons-alpha (IFN-alpha) and -gamma ( IFN-gamma ), interleukin-10 (IL-10) and -13 ( IL-13 ), transforming growth factor-beta1 ( TGF-beta1 ), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and tumor necrosis factor-alpha (TNF-alpha) on cell proliferation and induction of transcription factors AP-1 and NF-kappaB in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian carcinoma cells in vitro ... In the present study, we have investigated the effects of interferons-alpha (IFN-alpha) and -gamma ( IFN-gamma ), interleukin-10 (IL-10) and -13 ( IL-13 ), transforming growth factor-beta1 ( TGF-beta1 ), granulocyte-macrophage colony stimulating factor ( GM-CSF ), and tumor necrosis factor-alpha (TNF-alpha) on cell proliferation and induction of transcription factors AP-1 and NF-kappaB in UM-EC-3 human endometrial adenocarcinoma cells and UT-OC-5 ovarian carcinoma cells in vitro
Warren et al., Mol Immunol 1999 : EMSA with crude nuclear extracts demonstrated that stimulation with CD40L results in the induction of NF-kappaB complexes that bind to each of the three NF-kappaB sites and are composed mainly of p50 and RelB, but also include c-Rel and p65
Bretschneider et al., Br J Pharmacol 1999 : Activation of NFkappaB via either PAR-1 or PAR-2 does not predict mitogenesis ... Activation of NFkappaB via either PAR-1 or PAR-2 does not predict mitogenesis
Newton et al., J Biol Chem 1999 (Breast Neoplasms) : Overexpression of IkappaBalpha but not IkappaBbeta1 resulted in reduced constitutive DNA binding of NF-kappaB in MDA-MB-231 cells ... Overexpression of IkappaBalpha but not IkappaBbeta1 resulted in reduced constitutive DNA binding of NF-kappaB in MDA-MB-231 cells ... Unexpectedly, IkappaBbeta1 overexpression moderately increased 12-O-tetradecanoylphorbol-13-acetate- and interleukin-1-inducible NF-kappaB DNA binding
Gil et al., Mol Cell Biol 1999 : Biochemical analysis and transient assays revealed that PKR expression by a VV vector induced NF-kappaB binding and transactivation
Sizemore et al., Mol Cell Biol 1999 : Specific PI3K inhibitors strongly inhibit both PI3K activation and NF-kappaB dependent gene expression but have no effect on the IL-1 stimulated degradation of IkappaBalpha, the nuclear translocation of NF-kappaB , or the ability of NF-kappaB to bind to DNA ... In contrast, PI3K inhibitors block the IL-1 stimulated phosphorylation of NF-kappaB itself, especially the p65/RelA subunit ... In contrast, PI3K inhibitors block the IL-1 stimulated phosphorylation of NF-kappaB itself, especially the p65/RelA subunit ... In contrast, PI3K inhibitors block the IL-1 stimulated phosphorylation of NF-kappaB itself, especially the p65/RelA subunit
Watanabe et al., Cell Signal 1999 : However, PDTC did not inhibit tumour necrosis factor-alpha (TNF-alpha) induced NF-kappaB DNA binding activity but potentiated the effect of TNF-alpha on kappaB dependent gene expression
Marx et al., Circulation 1999 (Arteriosclerosis) : Transient transfection of deletional VCAM-1 promoter constructs and electrophoretic mobility shift assays suggest that fenofibrate inhibits VCAM-1 transcription in part by inhibiting NF-kappaB
Hu et al., J Leukoc Biol 1999 : Using human fetal microglial cell cultures, we found that the gram negative bacterial cell wall component lipopolysaccharide (LPS) stimulated RANTES ( regulated upon activation of normal T cell expressed and secreted ) production through the protein kinase C signaling pathway and that activation of transcription nuclear factor (NF)-kappaB was required for this effect ... Anti-inflammatory cytokines, IL-10, and transforming growth factor ( TGF ) -beta sequentially inhibited LPS- and cytokine induced microglial cell NF-kappaB activation, RANTES mRNA expression, and protein release ... Anti-inflammatory cytokines, IL-10 , and transforming growth factor ( TGF ) -beta sequentially inhibited LPS- and cytokine induced microglial cell NF-kappaB activation, RANTES mRNA expression, and protein release
Wilson et al., Gastroenterology 1999 : In contrast, IL-1beta induced NF-kappaB activation was associated with the disappearance of IkappaBalpha and was inhibited by ALLN but not staurosporine ... In contrast, IL-1beta induced NF-kappaB activation was associated with the disappearance of IkappaBalpha and was inhibited by ALLN but not staurosporine
Schneider et al., Eur J Immunol 1999 : In contrast to TNF, TWEAK does only modestly activate NF-kappaB or c-jun N-terminal kinase ( JNK ) in Kym-1 cells
Lauzurica et al., Eur J Immunol 1999 (Disease Models, Animal...) : The in vivo effect of PDTC on NF-kappaB activation in liver tissues involved the inhibition of both LPS induced I kappaB-alpha degradation and the translocation of the p50 and p65 NF-kappaB subunits to the nucleus
Schwenzer et al., J Biol Chem 1999 : The human tumor necrosis factor (TNF) receptor associated factor 1 gene ( TRAF1 ) is up-regulated by cytokines of the TNF ligand family and modulates TNF induced activation of NF-kappaB and c-Jun N-terminal kinase ... Because it has been shown that members of the TRAF family are involved in activation of NF-kappaB and the c-Jun N-terminal kinase (JNK) by the interleukin-1 receptor and members of the TNF receptor superfamily, a role of TRAF1 in receptor cross-talk and/or feedback regulation of activated receptor signaling complexes can be suggested ... In fact, we found that TNF induced activation of JNK is prolonged in transfectants overexpressing TRAF1, whereas overexpression of a deletion mutant of TRAF1 in which the N-terminal part had been replaced by the green fluorescent protein interfered with TNF induced activation of NF-kappaB and JNK
Aupperle et al., J Immunol 1999 (Arthritis, Rheumatoid) : NF-kappa B regulation by I kappa B kinase in primary fibroblast-like synoviocytes ... To determine whether IKK regulates NF-kappa B in primary cells isolated from a site of human disease, we characterized IKK in cultured fibroblast-like synoviocytes ( FLS ) isolated from synovium of patients with RA or osteoarthritis ... Transfection with a dominant negative IKK-2 mutant prevented TNF-alpha mediated NF-kappa B nuclear translocation, whereas a dominant negative IKK-1 mutant had no effect
Jimi et al., J Immunol 1999 : sODF, as well as IL-1 , activated NF-kappaB and c-Jun N-terminal protein kinase (JNK) in OCLs
Stephens et al., Endocrinology 1999 (Insulinoma...) : TNF induced NF-kappaB activation in both primary islet cells and NIT-1 cells
Heyninck et al., J Cell Biol 1999 : We show that A20 does not inhibit TNF- induced nuclear translocation and DNA binding of NF-kappaB, although it completely prevents the TNF- induced activation of an NF-kappaB dependent reporter gene, as well as TNF induced IL-6 and granulocyte macrophage-colony stimulating factor gene expression ... We show that A20 does not inhibit TNF- induced nuclear translocation and DNA binding of NF-kappaB, although it completely prevents the TNF- induced activation of an NF-kappaB dependent reporter gene, as well as TNF induced IL-6 and granulocyte macrophage-colony stimulating factor gene expression ... We show that A20 does not inhibit TNF- induced nuclear translocation and DNA binding of NF-kappaB , although it completely prevents the TNF- induced activation of an NF-kappaB dependent reporter gene, as well as TNF induced IL-6 and granulocyte macrophage-colony stimulating factor gene expression ... Moreover, NF-kappaB activation induced by overexpression of the TNF receptor associated proteins TNF receptor associated death domain protein ( TRADD ), receptor interacting protein ( RIP ), and TNF recep- tor associated factor 2 ( TRAF2 ) was also inhibited by expression of A20, whereas NF-kappaB activation induced by overexpression of NF-kappaB inducing kinase (NIK) or the human T cell leukemia virus type 1 ( HTLV-1 ) Tax was unaffected
Bouloumie et al., FASEB J 1999 (Arteriosclerosis) : NF-kappaB , another redox-sensitive transcription factor, was also activated by leptin stimulation in an oxidant dependent manner
Yoshidome et al., Hepatology 1999 (Edema...) : The objective of the current study was to determine whether IL-10 could suppress hepatic ischemia/reperfusion induced NFkappaB activation and the ensuing inflammatory liver injury ... IL-10 suppressed NFkappaB activation as well as messenger RNA expression of tumor necrosis factor-alpha (TNF-alpha) and macrophage inflammatory protein-2 ( MIP-2 )
Simpson et al., J Neurochem 1999 : SNAP also enhanced nuclear protein expression of the p65 subunit of NF-kappaB ... This study indicates that NO-releasing compounds can directly induce nuclear NF-kappaB subunit expression in rat striatum and that glucocorticoids selectively inhibit p65 subunit expression following exposure to NO
Azuma et al., Exp Cell Res 1999 : TGF-beta1 inhibits NF-kappaB activity through induction of IkappaB-alpha expression in human salivary gland cells : a possible mechanism of growth suppression by TGF-beta1 ... Because NF-kappaB is not only involved in inflammatory responses but also mediates cell growth, we have investigated the effect of TGF-beta1 on the activity of NF-kappaB and the role of the inhibitory IkappaB-alpha protein in the growth of the human salivary gland cell clones NS-SV-AC, HSGc, and cl-1 ... These results indicate that TGF-beta1 downregulates NF-kappaB activity through the induction of IkappaB-alpha expression in human salivary gland cells and that inhibition of NF-kappaB activity suppresses the growth rate of these cells
Asschert et al., Int J Cancer 1999 (Ovarian Neoplasms) : Nuclear factor kappa-B (NF-KB) , which consisted mainly of p65-NF-KB was induced in response to TNF-alpha stimulation ... TNF-alpha stimulation enhanced AP-1 and induced NF-KB but no NF-IL6 DNA binding in these cells
Arima et al., Exp Hematol 1999 (Acute Disease...) : Tax induction of NF-kappaB is presumed to be involved in proliferation and activation of primary leukemia cells in vivo
Shi et al., Mol Cell Biochem 1999 : This activation of NF-kappaB was decreased by a metal chelator, diethylene triaminepentaacetic acid or a H2O2 scavenger, catalase, but was increased by superoxide dismutase
Sugiyama et al., J Biol Chem 1999 : IkappaBalphaM was resistant to stimulus dependent degradation and suppressed NF-kappaB activation induced by TNF-alpha ( 10 ng/ml ) or IL-1beta ( 10 ng/ml ) ... IkappaBalphaM was resistant to stimulus dependent degradation and suppressed NF-kappaB activation induced by TNF-alpha ( 10 ng/ml ) or IL-1beta ( 10 ng/ml )
Diaz-Meco et al., J Biol Chem 1999 : NF-kappaB activation by tumor necrosis factor alpha (TNFalpha) provides a survival signal that impairs the TNFalpha induced apoptotic response ... In addition, the down-regulation of Par-4 levels by oncogenic Ras sensitizes cells to TNFalpha induced NF-kappaB activation
Huttunen et al., J Biol Chem 1999 (Neuroblastoma) : Both the RAGE mediated neurite outgrowth and activation of NF-kappaB are blocked by deletion of the cytoplasmic domain of RAGE ... In contrast, the activation of NF-kappaB is inhibited by dominant negative Ras but not Rac or Cdc42 ... In contrast, the activation of NF-kappaB is inhibited by dominant negative Ras but not Rac or Cdc42
Nasuhara et al., J Biol Chem 1999 : This was consistent with IkappaBalpha phosphorylation and degradation, yet TNF-alpha induced NF-kappaB DNA binding was only 30-40 % greater than for IL-1beta
Chen et al., Am J Pathol 1999 : Nitric oxide inhibits HIV tat induced NF-kappaB activation ... In the present study, we demonstrate that HIV Tat activates NF-kappaB and that this activation can be attenuated by endogenous or exogenous NO ... Inhibition of endogenous NO production with the NO synthase (NOS) inhibitor L-NMMA causes a significant increase in Tat induced NF-kappaB activity ... In addition, NO attenuates signal initiated degradation of IkappaBalpha , an intracellular inhibitor of NF-kappaB , and blocks the DNA binding activity of the NF-kappaB p50/p50 homodimer and p50/p65 heterodimer
Tacchini et al., J Cell Physiol 1999 (Reperfusion Injury) : 3 ) Reperfusion, which is known to further increase the binding of HSF and to induce NFkappaB binding, abrogates or decreases the binding of HIF-1 and AP-1, stimulated by ischemia, and activates the binding of ATF/CREB
Revy et al., J Immunol 1999 : However, the components of the NF-kappaB complexes were different in monocytes and B cells, because p50 is part of the NF-kappaB complex induced by CD40 triggering in both monocytes and B cells, whereas p65 was only induced in B cells
Thomas et al., J Immunol 1999 (Hypersensitivity, Delayed...) : Activation of IRAK leads in turn to nuclear translocation of NF-kappaB , which directs expression of innate and adaptive immune response genes
Costanzo et al., J Biol Chem 1999 : Expression of c-E10 under the control of a doxycycline dependent transcriptional transactivator results in NF-kappaB activation, which is inhibited by dominant negative forms of TRAF2 and NIK kinase
Chen et al., J Biol Chem 1999 : Vanadate induction of NF-kappaB involves IkappaB kinase beta and SAPK/ERK kinase 1 in macrophages
Reuther et al., J Biol Chem 1999 : Our data show that DeltaN-IkappaBalpha can bind NF-kappaB, suppress NF-kappaB activation, and sensitize cells to death
Demarchi et al., J Virol 1999 : Tat mediated NF-kappaB activation and transcriptional induction of the HIV-1 long terminal repeat were impaired in murine cells in which the PKR gene was knocked out ... Activation of NF-kappaB by Tat required integrity of the basic domain of Tat ; previous studies have indicated that this domain is necessary for specific Tat-PKR interaction
Jue et al., J Korean Med Sci 1999 (Arthritis, Rheumatoid) : The nuclear factor kappaB (NF-kappaB) plays an essential role in transcriptional activation of TNF and IL-1 ... The nuclear factor kappaB (NF-kappaB) plays an essential role in transcriptional activation of TNF and IL-1 ... Aspirin and sodium salicylate inhibit activation of NF-KB by blocking IkappaB kinase , a key enzyme in NF-kappaB activation
Bendall et al., Mol Immunol 1999 : Whereas expression of the inhibitor had only modest effects on basal or LPS induced levels of NF-kappaB , transgenic B cells were significantly impaired for cellular proliferation and NF-kappaB induction in response to B cell receptor (BCR) crosslinking
Chaqour et al., J Mol Cell Cardiol 1999 : These data strongly suggest that stretch induced PAF receptor gene expression is mediated by NF-kappaB binding to the PAF receptor gene promoter and that protein kinase C activation is among the molecular features of NF-kappaB activation and translocation into the nucleus in mechanically stretched cells
Mori et al., J Cell Biochem 1999 : Several lines of evidence suggest that TNF alpha activates transcription factor nuclear factor-kappa B (NF kappa B) through activation of protein kinase C ( PKC ) or the hydrolysis of sphingomyelin to ceramide in a number of cell types
Neumann et al., FEBS Lett 1999 : Hyaluronic acid with a molecular weight above 1.2 MDa ( HMW-HA ) inhibits the AGE induced activation of the transcription factor NF-kappaB and the NF-kappaB regulated cytokines interleukin-1alpha, interleukin-6 and tumor necrosis factor-alpha
Zhu et al., Atherosclerosis 1999 : LDL itself does not increase NF-kappa B binding in endothelial cells ( ECs )
Han et al., Am J Physiol 1999 : Cholecystokinin induction of mob-1 chemokine expression in pancreatic acinar cells requires NF-kappaB activation
Izumi et al., Mol Cell Biol 1999 (Burkitt Lymphoma...) : Further, LMP1 does not require TRADD residues 294 to 312 for NF-kappaB activation, while TNFR1 requires TRADD residues 296 to 302 ... Surprisingly, LMP1 does not require RIP for NF-kappaB activation
Guttridge et al., Mol Cell Biol 1999 (Cell Transformation, Neoplastic) : An analysis of cell cycle markers revealed that NF-kappaB activates cyclin D1 expression, and the results showed that this regulatory pathway is one mechanism by which NF-kappaB inhibits myogenesis ... NF-kappaB regulation of cyclin D1 occurs at the transcriptional level and is mediated by direct binding of NF-kappaB to multiple sites in the cyclin D1 promoter ... Using diploid fibroblasts, we demonstrate that NF-kappaB is required to induce cyclin D1 expression and pRb hyperphosphorylation and promote G ( 1 ) -to-S progression ... Using diploid fibroblasts, we demonstrate that NF-kappaB is required to induce cyclin D1 expression and pRb hyperphosphorylation and promote G ( 1 ) -to-S progression
Ichiyama et al., Brain Res 1999 (Brain Diseases...) : The effects of alpha-MSH on NF-kappaB and IkappaBalpha were paralleled by pretreatment with alpha-MSH11-13
Maulik et al., Ann N Y Acad Sci 1999 (Myocardial Ischemia...) : In concert, the adapted myocardium is associated with increased antioxidant gene Bcl-2 , increased binding activity of the nuclear transcription factor NF kappa B , and reduced binding activity of AP-1 compared to nonadapted hearts
Duffey et al., Cancer Res 1999 (Carcinoma, Squamous Cell...) : In UM-SCC-9 cells that stably expressed IkappaBalphaM, inhibition of constitutive and tumor necrosis factor-a induced NF-kappaB activation, and production of all four cytokines was observed
Dong et al., Cancer Res 1999 (Carcinoma, Squamous Cell...) : The increased cytokine expression was associated with an increase in constitutive and TNF-alpha-inducible activation of NF-kappaB as demonstrated by electrophoretic mobility shift assay and luciferase-reporter gene assay ... Induction of NF-kappaB by TNF-alpha was inhibited by the addition of protease inhibitors calpain inhibitor I and N-tosyl-phechloromethyl ketone and antioxidant 1-pyrrolidinecarbodithioic acid, whereas constitutive activation of NF-kappaB and cytokine KC mRNA expression was inhibited by N-tosyl-phechloromethyl ketone alone ... Overexpression of a human Ikappa(B)alpha dominant suppresser in Pam 212 cells inhibited TNF-alpha induced NF-kappaB binding activity and KC expression ... These data indicate that activation of NF-kappaB contributes to increased expression of proinflammatory cytokines during metastatic tumor progression of squamous cell carcinoma, and that distinct mechanisms may be involved in the regulation of constitutive and TNF-alpha induced activation of NF-kappaB in squamous cell carcinoma
Holden et al., Med Hypotheses 1999 (Disease Progression...) : In addition, it has been established that tumor necrosis factor-alpha (TNF-alpha) can activate the expression of wild type p53 in concert with the nuclear transcription factor, NF-kappa B
Mori et al., Infect Immun 1999 : Furthermore, nitrite reductase induced the degradation of IkappaBalpha , the major cytoplasmic inhibitor of NF-kappaB , and the expression of IkappaBalpha mRNA
Desai et al., Lab Invest 1999 (Disease Models, Animal...) : Because in vitro expression of MCP-1 is in part mediated by the redox-sensitive transcription factors nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1), we studied their activation as a function of varying intracellular GSH redox status in the pathogenesis of glucan induced pulmonary granulomatosis
Shao et al., J Biol Chem 1999 (Ovarian Neoplasms...) : Since activation of nuclear factor kappaB (NF-kappaB) has been shown to play an anti-apoptotic role in TNF induced apoptosis, we examined apoptotic susceptibility and NF-kappaB activation induced by TNF in the E1A transfectants and their parental cells
Shimada et al., Int Immunol 1999 (Inflammation) : Overexpression of wild-type IKK-i phosphorylated serine residues Ser32 and Ser36 of IkappaB-alpha ( preferentially Ser36 ), and significantly stimulated NF-kappaB activation
Boyce et al., Bone 1999 : IL-1 and tumor necrosis factor in turn also regulate the expression of NF-kappaB ... IL-1 and tumor necrosis factor in turn also regulate the expression of NF-kappaB
Sakurai et al., FEBS Lett 1999 : Regulatory role of metallothionein in NF-kappaB activation
Alpert et al., J Biol Chem 1999 : Thus, sustained p38 activation by various stimuli inhibits TNF induced IkappaB phosphorylation and NF-kappaB activation ... Thus, sustained p38 activation by various stimuli inhibits TNF induced IkappaB phosphorylation and NF-kappaB activation
Leo et al., J Biol Chem 1999 : Differential requirements for tumor necrosis factor receptor associated factor family proteins in CD40 mediated induction of NF-kappaB and Jun N-terminal kinase activation ... Taken together, these findings reveal redundancy in the involvement of TRAF family proteins in CD40 mediated NF-kappaB induction and suggest that the membrane-proximal region of CD40 may stimulate the JNK pathway through a TRAF independent mechanism ... A carboxyl-truncation mutant of CD40 lacking the last 32 amino acids required for TRAF2 and TRAF3 binding, CD40 ( Delta32 ), mediated NF-kappaB induction through a mechanism that was suppressible by co-expression of TRAF6 ( DeltaN ), a dominant negative version of TRAF6, but not by TRAF2 ( DeltaN ), implying that while TRAF6 does not directly bind CD40, it can participate in CD40 signaling ... A carboxyl-truncation mutant of CD40 lacking the last 32 amino acids required for TRAF2 and TRAF3 binding, CD40 ( Delta32 ), mediated NF-kappaB induction through a mechanism that was suppressible by co-expression of TRAF6 ( DeltaN ), a dominant negative version of TRAF6, but not by TRAF2 ( DeltaN ), implying that while TRAF6 does not directly bind CD40, it can participate in CD40 signaling
Okabe et al., Jpn J Cancer Res 1999 : Moreover, SS555 inhibited TNF-alpha gene expression mediated through inhibition of AP-1 activation, but not NF-kappa B activation
Wright et al., J Cell Physiol 1999 : IL-1beta and TNF-alpha stimulated nuclear NF-kappaB levels by about fourfold and fivefold, respectively, in HASMCs ... IL-1beta and TNF-alpha stimulated nuclear NF-kappaB levels by about fourfold and fivefold, respectively, in HASMCs ... These data suggest that a significant increase in nuclear NF-kappaB levels is not necessary or sufficient for VCAM-1 upregulation in HASMCs and does not determine the relative sensitivity to inhibition of gene expression by PDTC
Lee et al., Proc Natl Acad Sci U S A 1999 (Lymphoma, B-Cell) : NF-kappaB mediated up-regulation of Bcl-x and Bfl-1/A1 is required for CD40 survival signaling in B lymphocytes
Yamakawa et al., Endocrinology 1999 : Different types of NF-kappaB inhibitors, pyrrolidine dithiocarbamate and MG132, which specifically abolished IkappaB degradation and subsequent NF-kappaB activation by LPS , suppressed TNF alpha secretion from VSMC
Hamanoue et al., Mol Cell Neurosci 1999 : p75 mediated NF-kappaB activation enhances the survival response of developing sensory neurons to nerve growth factor ... Overexpression of either the p65 or the p50 NF-kappaB subunits resulted in NF-kappaB activation and promoted in vitro survival as effectively as NGF ... Expression of a superrepressor IkappaB-alpha protein prevented NF-kappaB activation in p65/p50 overexpressing neurons and caused the neurons to die as rapidly as NGF deprived neurons ... Antibodies that block binding of NGF to the p75 receptor prevented NGF induced NF-kappaB activation and reduced the NGF survival response to the same extent as superrepressor IkappaB-alpha
Matsui et al., Cardiovasc Res 1999 (Anoxia) : Induction of interleukin (IL)-6 by hypoxia is mediated by nuclear factor (NF)-kappa B and NF-IL6 in cardiac myocytes
Curry et al., J Biol Chem 1999 : These experiments demonstrate that heat shock transiently inhibits IR induction of NF-kappaB DNA binding activity by preventing IKK activation and suggests a mechanism independent of protein synthesis
Chêne et al., J Virol 1999 : TNF is the major inducer of NF-kappaB and particularly of the p50-p65 complex, whereas IL-7 acts as a cofactor by sustaining the expression of the p75 TNF receptor
Bourke et al., J Immunol 1999 (Astrocytoma...) : However, dexamethasone failed to induce I kappa B or inhibit activation of NF-kappa B by IL-1 in the two cell types
Amrani et al., J Immunol 1999 : Using EMSA, we show that stimulation with TNF-alpha or IL-1 beta induces NF-kappa B DNA binding activity in human airway smooth muscle cells ... Using EMSA, we show that stimulation with TNF-alpha or IL-1 beta induces NF-kappa B DNA binding activity in human airway smooth muscle cells ... In cells transfected with the luciferase reporter, dexamethasone did not affect TNF-alpha induced NF-kappa B-dependent transcription
Ray et al., J Immunol 1999 (Acute Disease...) : Persistent expression of serum amyloid A during experimentally induced chronic inflammatory condition in rabbit involves differential activation of SAF, NF-kappa B , and C/EBP transcription factors ... This result suggested that C/EBP, SAF, and NF-kappa B are required for transient acute phase induction of SAA whereas SAF and NF-kappa B activities are necessary for persistent SAA expression during chronic inflammatory conditions
Manna et al., Oncogene 1999 : Transfection of cells with gamma-GCS cDNA blocked TNF induced NF-kappa B activation , cytoplasmic I kappa B alpha degradation, nuclear translocation of p65, and NF-kappa B-dependent gene transcription ... gamma-GCS overexpression also completely suppressed NF-kappa B activation induced by phorbol ester and okadaic acid, whereas that induced by H2O2, ceramide, and lipopolysaccharide was minimally affected
Li et al., Gene Expr 1999 : Tax also induces a variety of cellular genes through activation of the transcription factor NF-kappaB
Wang et al., Ann Clin Lab Sci 1999 : The role of superoxide radical in TNF-alpha induced NF-kappaB activation ... Superoxide dismutase ( SOD ) , but not catalase or sodium formate, inhibited this NF-kappaB activation, suggesting that O2*- rather than H2O2 or *OH, radicals play the most critical role in this induction ... Superoxide dismutase ( SOD ), but not catalase or sodium formate, inhibited this NF-kappaB activation, suggesting that O2*- rather than H2O2 or *OH, radicals play the most critical role in this induction ... 02*- seems to play a key role in TNF-alpha induced NF-kappaB activation in macrophages ... Xanthine and xanthine oxidase appears to be a source of O2*- radicals responsible for TNF-alpha induced NF-kappaB activation
Tsai et al., J Cell Biochem 1999 : Suppression of TNFalpha mediated NFkappaB activity by myricetin and other flavonoids through downregulating the activity of IKK in ECV304 cells
Puls et al., J Cell Sci 1999 (Inflammation) : When expressed in fibroblasts, LMP1 stimulates Cdc42 dependent filopodia formation as well as JNK and NF(kappa)B activation ... Using LMP1 mutants, we show that activation of Cdc42 and JNK/NF(kappa)B occur through distinct pathways and that Cdc42 activation is independent of LMP1 's interaction with TRADD and TRAF proteins ... Using LMP1 mutants, we show that activation of Cdc42 and JNK/NF(kappa)B occur through distinct pathways and that Cdc42 activation is independent of LMP1 's interaction with TRADD and TRAF proteins ... Using LMP1 mutants, we show that activation of Cdc42 and JNK/NF(kappa)B occur through distinct pathways and that Cdc42 activation is independent of LMP1 's interaction with TRADD and TRAF proteins
Greene et al., Biochim Biophys Acta 1999 : Interleukin-1 receptor associated kinase, IRAK, has been shown to activate NFkappaB in response to interleukin-1
Polazzi et al., J Neuropathol Exp Neurol 1999 : Indeed, the induction of the iNOS gene by Tat was largely prevented by a specific inhibitor of the nuclear factor-kB (NF-kB) , a transcription factor known to be involved in the induction of iNOS by LPS
Hehner et al., Eur J Biochem 1999 : Pervanadate induced tyrosine phosphorylation increased the TNF-alpha induced cytotoxicity and NF-kappaB activation, suggesting that it stimulates early signaling events prior to the separation of the two signaling pathways
Chen et al., Immunology 1999 : LPS activation of nuclear factor-kappa B (NF-kappaB) , activator protein-1 (AP-1) and p38 MAPK was also inhibited by SB 203580
Shi et al., Biochem Biophys Res Commun 1999 : Purified recombinant CLC induced the activation of NFkappaB and SRE reporter constructs in the TF-1, U937, and M1 cell lines
Oliver et al., EMBO J 1999 (Shock, Septic) : The molecular basis for this resistance relies on an almost complete abrogation of NF-kappaB dependent accumulation of TNF-alpha in the serum and a down-regulation of inducible nitric oxide synthase (iNOS) , leading to decreased NO synthesis, which is the main source of free radical generation during inflammation ... The molecular basis for this resistance relies on an almost complete abrogation of NF-kappaB dependent accumulation of TNF-alpha in the serum and a down-regulation of inducible nitric oxide synthase (iNOS), leading to decreased NO synthesis, which is the main source of free radical generation during inflammation
Nakano et al., Proc Natl Acad Sci U S A 1999 : Activation of either NF-kappaB or c-Jun NH ( 2 ) -terminal kinase/stress activated protein kinase by tumor necrosis factor , CD27, and CD40 was not abrogated in traf5 ( -/- ) mice ... Activation of either NF-kappaB or c-Jun NH ( 2 ) -terminal kinase/stress activated protein kinase by tumor necrosis factor, CD27, and CD40 was not abrogated in traf5 ( -/- ) mice ... Activation of either NF-kappaB or c-Jun NH ( 2 ) -terminal kinase/stress activated protein kinase by tumor necrosis factor, CD27 , and CD40 was not abrogated in traf5 ( -/- ) mice
Ten et al., J Allergy Clin Immunol 1999 : CD23 induced NF-kappaB is a heterodimer composed of p65/p50 subunits
Sheppard et al., Mol Cell Biol 1999 : Like nuclear receptor dependent gene expression, NF-kappaB dependent gene expression requires specific LXXLL motifs in one of the p160 family members, and enhancement of NF-kappaB activity requires the histone acetyltransferase ( HAT ) activity of p/CAF but not that of CBP
Rohr et al., Nucleic Acids Res 1999 : However, in contrast with TNF-alpha , dopamine treatment did not affect NF-kappaB DNA binding activity nor the concentrations of p50, p65 and IkappaB-alpha proteins, which suggests a distinct NF-kappaB activation mechanism
Belt et al., Am J Obstet Gynecol 1999 : The nuclear transcription factor NF-kappaB mediates interleukin-1beta induced expression of cyclooxygenase-2 in human myometrial cells ... In this study we investigated the possible role of the nuclear transcription factor NF-kappaB in interleukin-1 signaling, leading to the expression of cyclooxygenase 2 and prostaglandin production in human myometrial cell cultures ... Stimulation of human myometrial cells with interleukin 1 leads to rapid activation of the transcription factor NF-kappaB , which is functionally linked to the expression of cyclooxygenase 2 messenger ribonucleic acid, protein, and prostaglandin synthesis
Lo et al., Shock 1999 : In comparison, sphingosine inhibited TNF mRNA expression as well as TNF production of LPS stimulated MO. LPS induced MO NFkappaB activity was also reduced by sphingosine
Taylor et al., Surgery 1999 : Previously, we showed that maximal inducible nitric oxide synthase (iNOS) expression is up-regulated by a combination of cytokines and that this effect is mediated by the transcription factor NF-kappa B
Raychaudhuri et al., Am J Respir Cell Mol Biol 1999 (Asthma...) : In vitro studies with AM showed that NF-kappaB activation by lipopolysaccharide (LPS) is decreased by NO in a dose dependent manner
Simeonova et al., Lab Invest 1999 : Furthermore using cell transfection and mobility shift assays, it was found that transcriptional activation of the IL-8 gene required TNF-alpha induced activation and binding of nuclear factor-kappaB (NF-kappaB)- and NF-IL-6, nuclear transcription factors to regulatory elements in the IL-8 promoter ... Furthermore using cell transfection and mobility shift assays, it was found that transcriptional activation of the IL-8 gene required TNF-alpha induced activation and binding of nuclear factor-kappaB (NF-kappaB)- and NF-IL-6, nuclear transcription factors to regulatory elements in the IL-8 promoter
Wooten et al., Cell Death Differ 1999 : Furthermore, transfection of full-length antisense PKC-iota blocked basal and NGF stimulated NF-kappaB
Heissmeyer et al., EMBO J 1999 : Furthermore, a p105 mutant, lacking the IKK phosphorylation sites, acts as a super-repressor of IKK induced NF-kappaB transcriptional activity
Fiebich et al., Neuropharmacology 1999 (Alzheimer Disease) : We show that inhibition of NF-kappaB activation by tepoxalin is mediated by preventing IkappaB-alpha degradation
Egan et al., J Biol Chem 1999 : Inhibition of interleukin-1 stimulated NF-kappaB RelA/p65 phosphorylation by mesalamine is accompanied by decreased transcriptional activity
Hu et al., J Immunol 1999 (Leukemia, Erythroblastic, Acute...) : TNF-alpha rapidly induced marked activation of nuclear transcription factor NF-kappa B in all 4 cell lines
Ohm et al., J Immunol 1999 : VEGF inhibited FL-inducible activation of transcription factor NF-kappaB
Jobin et al., J Immunol 1999 (Inflammation) : However, I kappa B alpha degradation induced by ectopically expressed NF-kappa B-inducing kinase or IKK was not inhibited by curcumin treatment
Hawiger et al., Blood 1999 (Inflammation) : Thus, IKK complex constitutes the main intracellular target for LPS induced NF-kappaB signaling to the nucleus in human monocytic cells to activate genes responsible for septic shock
Vlahopoulos et al., Blood 1999 : TNFalpha treatment induces a rapid translocation of the 65 kD transcriptional activator NF-kappaB subunit, Rel A, whose binding in the nucleus occurs before changes in intracellular ROS
Matthews et al., Cytokine 1999 (Thymoma...) : Neither PD98059 nor SB203580 had an inhibitory effect on NFkappaB-driven reporter gene expression in response to IL-1
Blázquez et al., AIDS Res Hum Retroviruses 1999 : Extracellular HIV type 1 Tat protein induces CD69 expression through NF-kappaB activation : possible correlation with cell surface Tat binding proteins ... Tat induces NF-kappaB binding to DNA as a result of IkappaBalpha phosphorylation and degradation, which depend on the intracellular redox state ... Tat induces NF-kappaB binding to DNA as a result of IkappaBalpha phosphorylation and degradation, which depend on the intracellular redox state
Ching et al., Biochem Pharmacol 1999 : DMXAA and FAA induced NFkappaB translocation with slower kinetics of activation compared with LPS
Kobuchi et al., Am J Physiol 1999 : Quercetin had no effect on PMA- or TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation
Schroeder et al., Am J Physiol 1999 : On the basis of previous work demonstrating nitric oxide ( NO ) -mediated inhibition of nuclear factor-kappaB (NF-kappaB) DNA binding, we hypothesized that NO downregulates NF-kappaB dependent interleukin-1beta (IL-1beta) production in an ANA-1 macrophage model of lipopolysaccharide (LPS) stimulation
Tando et al., Am J Physiol 1999 (Second Messenger Systems) : Caerulein induced NF-kappaB/Rel activation requires both Ca2+ and protein kinase C as messengers ... Here, we show that activation of NF-kappaB/Rel by caerulein, a CCK analog, requires increasing intracellular Ca2+ levels and protein kinase C activation
Otsuka et al., Cancer Res 1999 (Glioma) : Activation of NF-kappaB by TNF-alpha in the resistant cell lines resulted in a significant increase of a reporter gene expression driven by NF-kappaB site, suggesting a possibility that activation of p50-p65 confers resistance to TNF-alpha
Ozes et al., Nature 1999 : NF-kappaB activation by tumour necrosis factor requires the Akt serine-threonine kinase ... Activation of the nuclear transcription factor NF-kappaB by inflammatory cytokines requires the successive action of NF-kappaB inducing kinase (NIK) and an IKB-kinase (IKK) complex composed of IKKalpha and IKKbeta ... Here we show that the Akt serine-threonine kinase is involved in the activation of NF-kappaB by tumour necrosis factor (TNF) ... Here we show that the Akt serine-threonine kinase is involved in the activation of NF-kappaB by tumour necrosis factor (TNF) ... Wortmannin ( a PI(3)K inhibitor ), dominant negative PI(3)K or kinase-dead Akt inhibits TNF mediated NF-kappaB activation ... Wortmannin ( a PI(3)K inhibitor ), dominant negative PI(3)K or kinase-dead Akt inhibits TNF mediated NF-kappaB activation ... Wortmannin ( a PI(3)K inhibitor ), dominant negative PI(3)K or kinase-dead Akt inhibits TNF mediated NF-kappaB activation ... Constitutively active Akt induces NF-kappaB activity and this effect is blocked by dominant negative NIK ... Conversely, NIK activates NF-kappaB and this is blocked by kinase-dead Akt ... Thus, both Akt and NIK are necessary for TNF activation of NF-kappaB ... Thus, both Akt and NIK are necessary for TNF activation of NF-kappaB ... Mutation of this amino acid blocks phosphorylation by Akt or TNF and activation of NF-kappaB ... Mutation of this amino acid blocks phosphorylation by Akt or TNF and activation of NF-kappaB
Tsuji-Takayama et al., Cell Immunol 1999 : Interleukin-18 induces interferon-gamma production through NF-kappaB and NFAT activation in murine T helper type 1 cells ... Interleukin-18 induces interferon-gamma production through NF-kappaB and NFAT activation in murine T helper type 1 cells ... We conclude that dual signaling consisting of IL-18 induced NF-kappaB activation and TCR/CD3 mediated NFAT activation is crucial for IFN-gamma production by IL-18 in murine Th1 cells ... We conclude that dual signaling consisting of IL-18 induced NF-kappaB activation and TCR/CD3 mediated NFAT activation is crucial for IFN-gamma production by IL-18 in murine Th1 cells
Cheng et al., Exp Mol Pathol 1999 : Role of transcription factor NF-kappaB in asbestos induced TNFalpha response from macrophages ... Inhibition of NF-kappaB by SN50, an inhibitor of NF-kappaB nuclear translocation, or by sequence-specific oligonucleotides directed against the NF-kappaB binding site of TNFalpha promoter attenuated the asbestos effect on TNFalpha production
Hirota et al., J Biol Chem 1999 : In the nucleus, however, TRX enhances NF-kappaB transcriptional activities by enhancing its ability to bind DNA ... This two-step TRX dependent regulation of the NF-kappaB complex may be a novel activation mechanism of redox-sensitive transcription factors
Pazdernik et al., Mol Cell Biol 1999 : In vitro kinase assays demonstrate that mRIP3 is catalytically active and has autophosphorylation site ( s ) in the C-terminal domain, but the mRIP3 catalytic activity is not required for mRIP3 induced apoptosis and NF-kappaB activation
Chin et al., Mol Cell Biol 1999 (MAP Kinase Signaling System) : We previously described the involvement of a TRAF interacting molecule, TRAF associated NF-kappaB activator ( TANK ), in TRAF2 mediated NF-kappaB activation
Harhaj et al., Mol Cell Biol 1999 : Signal mediated IkappaBalpha degradation triggers the release and subsequent nuclear translocation of NF-kappaB
Shimada et al., J Pharmacol Exp Ther 1999 : Because IL-8 expression is regulated by the transcription factor nuclear factor-kappaB (NF-kappaB) , we also examined the effect of polaprezinc on NF-kappaB activity ... NF-kappaB activation in response to TNF-alpha , IL-1beta, phorbol ester, and H ( 2 ) O ( 2 ) was down-regulated by polaprezinc ... NF-kappaB activation in response to TNF-alpha, IL-1beta , phorbol ester, and H ( 2 ) O ( 2 ) was down-regulated by polaprezinc ... NF-kappaB activation in response to TNF-alpha, IL-1beta , phorbol ester, and H ( 2 ) O ( 2 ) was down-regulated by polaprezinc
Ten et al., J Immunol 1999 : Investigation of the second messengers mediating the CD23 dependent activation of NF kappa B demonstrates that I kappa B kinases ( IKKs ) but not p90rsk are selectively activated following CD23 cross linking and mediates the phosphorylation of I kappa B alpha ... Cotransfection experiments with an IKK beta negative dominant completely inhibit CD23 induced NF kappa B activation ... Furthermore, the activation of tyrosine kinase ( s ) by CD23 is required for the induction of IKK activity, I kappa B alpha degradation, and NF-kappa B nuclear translocation ... Taken together, our results show that CD23 cross linking in the monocytic lineage induces tyrosine kinase activation followed by activation of IKK, which phosphorylates I kappa B alpha at the N-terminal domain ( Ser32/Ser36 ), inducing its degradation, NF-kappa B activation and gene transcription
Pahlevan et al., J Immunol 1999 : The factor had no effect on TNF induced cytotoxicity, but abrogated TNF induced NF-kappa B activation ... The factor had no effect on IL-1- or LPS induced NF-kappa B activity, indicating selective activity of the factor
Wickremasinghe et al., J Immunol 1999 : Such IL-8 secretion is NF-kappa B dependent , NF-IL-6 requiring, and activated by an IL-1 mediated pathway as a consequence of phagocytosis of M. tuberculosis by monocytes
Liu et al., Circulation 1999 (Shock, Septic) : PDTC prevented the LPS induced NF-kappaB activation but did not inhibit activation of the transcription factors AP-1, Sp-1, and CREB
Lin et al., Proc Natl Sci Counc Repub China B 1999 (Cardiovascular Diseases...) : The activation of nuclear factor kappa B (NF kappa B) induced by lipopolysaccharide (LPS) was inhibited by resveratrol
Hassa et al., Biol Chem 1999 : Here we show that poly ( ADP ribose ) polymerase ( PARP ) is required for specific NF-kappaB transcriptional activation in vivo
Ye et al., Mol Cell Biochem 1999 : Induction of TNFalpha in macrophages by vanadate is dependent on activation of transcription factor NF-kappaB and free radical reactions ... This is supported by evidence that inhibition of NF-kappaB activation by SN50, a specific NF-kappaB inhibitor, resulted in a decrease in the TNFalpha production ... Taken together, these results indicate that : ( a ) activation of the TNFalpha gene promoter contributes to the vanadate induced TNFalpha production ; ( b ) NF-kappaB is required for the vanadate induced promoter activity of TNFalpha gene ; ( c ) free radical reactions are involved in the vanadate induced TNFalpha production and NF-kappaB activation
Sakaguchi et al., Hepatology 1999 (Drug-Induced Liver Injury...) : Both LPS and PAF activate nuclear factor-kappa B (NF-kappaB) , a key transcription factor for tumor necrosis factor-alpha (TNF-alpha) and cytokine induced neutrophil chemoattractant ( CINC ) ... Both LPS and PAF activate nuclear factor-kappa B (NF-kappaB) , a key transcription factor for tumor necrosis factor-alpha (TNF-alpha) and cytokine induced neutrophil chemoattractant ( CINC ) ... The aim of this study is to investigate how PAF participates in the LPS induced and NF-kappaB mediated regulation of TNF-alpha and CINC in regenerating rat livers ... Based on these results, we propose that PAF contributes to continuous CINC up-regulation and NF-kappaB activation via accumulation and activation of neutrophils, and thereby is involved in LPS induced liver injury in regenerating rat livers
Dechend et al., Circulation 1999 (Arteriosclerosis) : Chlamydia pneumoniae infection of vascular smooth muscle and endothelial cells activates NF-kappaB and induces tissue factor and PAI-1 expression : a potential link to accelerated arteriosclerosis
Lentsch et al., Gastroenterology 1999 (Inflammation...) : SLPI also suppressed activation of the transcription factor NF-kappaB in liver
Dodel et al., J Neurochem 1999 : Here we show that the neurotoxic A beta, a major plaque component, and lipopolysaccharides (LPS) , an immune reaction triggering portion of bacterial membranes, are both potent activators of the nuclear transcription factor NF-kappaB in primary rat astroglial cells
Hernández et al., J Neurochem 1999 (Astrocytoma...) : In keeping with these findings, TNF-alpha produced an increase of COX-2 expression, as judged from both RT-PCR studies and immunoblot of COX-2 protein, and a long lasting activation of NF-kappaB
Fowler et al., Biochem Cell Biol 1999 : Nitric oxide regulates interleukin-8 gene expression in activated endothelium by inhibiting NF-kappaB binding to DNA : effects on endothelial function
Ichiyama et al., J Neuroimmunol 1999 (Acute Disease...) : Electrophoretic mobility shift assays of nuclear extracts from the murine foot pad injected with TNF-alpha demonstrated that centrally administered alpha-MSH does inhibit NF-kappaB activation ... Centrally administered alpha-MSH inhibited peripheral NF-kappaB activation and IkappaBalpha degradation even in mice with nonfunctional melanocortin 1 receptors (MC1R) ... These findings indicate that alpha-MSH can act centrally to inhibit NF-kappaB activation in peripheral acute inflammation via a descending neural pathway
Liang et al., Carcinogenesis 1999 : Moreover, electrophoretic mobility shift assay ( EMSA ) experiments indicated that apigenin blocked the LPS induced activation of nuclear factor-kB (NF-kB)
Ondrey et al., Mol Carcinog 1999 (Carcinoma, Squamous Cell...) : We concluded that the early transcription factors NF-kappaB, AP-1, and NF-IL6 are constitutively activated in human HNSCC cell lines and that NF-kappaB and AP-1 promote expression of the pro-inflammatory and pro-angiogenic cytokine IL-8 in HNSCC
Wang et al., Br J Pharmacol 1999 (Brain Neoplasms...) : Results showed that nepalolide A diminished the LPS/IFN-gamma mediated association of NF-kappaB with consensus oligonucleotide in a concentration dependent manner ... Results showed that nepalolide A diminished the LPS/IFN-gamma mediated association of NF-kappaB with consensus oligonucleotide in a concentration dependent manner
Ye et al., J Biol Chem 1999 : TRAF4 also inhibited the NF-kappaB response, whereas TRAF2 and TRAF6 enhanced p75 ( NTR ) -induced NF-kappaB activation
Du et al., Free Radic Biol Med 1999 : Generation of reactive oxygen intermediates, activation of NF-kappaB , and induction of apoptosis in human endothelial cells by glucose : role of nitric oxide synthase ?
Estrov et al., Blood 1999 (Acute Disease...) : Phenylarsine oxide blocks interleukin-1beta induced activation of the nuclear transcription factor NF-kappaB , inhibits proliferation, and induces apoptosis of acute myelogenous leukemia cells ... Because PAO inhibits activation of the transcription factor NF-kappaB and because NF-kappaB modulates an array of signals controlling cellular survival, proliferation, and cytokine production, we also studied the effect of PAO on NF-kappaB activation in AML cells and found that PAO suppressed the IL-1beta induced activation of NF-kappaB
Kan et al., Am J Physiol 1999 : TNF-alpha enhances cardiac myocyte NO production through MAP kinase mediated NF-kappaB activation ... TNF-alpha enhanced MAP kinase activity was associated with an increase in IL-1beta stimulated NF-kappaB activity
Zhang et al., Am J Physiol 1999 : Nitric oxide induced reduction of lung cell and whole lung thioredoxin expression is regulated by NF-kappaB
Blackwell et al., Am J Physiol 1999 (Inflammation) : Therefore, IT LPS causes differential NF-kappaB activation in air space cells and lung tissue, which likely determines production of key cytokines and directs the evolution of neutrophilic alveolitis
Kondo et al., Free radical research 1999 : To know the regulation of the expression of gamma-GCS gene, in the present study, we show evidences that gamma-GCS heavy subunit is upregulated by oxidative stress by ionizing radiation and TNF-alpha mediated by nuclear factor-kappaB (NF-kappaB) , and impairment of the expression of gamma-GCS by TNF-alpha in diabetic condition
Lin et al., Genes Dev 1999 : We demonstrated that the cleavage of RIP resulted in the blockage of TNF induced NF-kappaB activation
Bertrand et al., J Biol Chem 1999 : We recently showed that the antiapoptotic function of insulin requires nuclear factor kappaB (NF-kappaB) activation ( Bertrand, F., Atfi, A., Cadoret, A., L'Allemain, G., Robin, H., Lascols, O., Capeau, J., and Cherqui, G. ( 1998 ) J. Biol. Chem. 273, 2931-2938 ) ... Along with its protective effect, overexpressed TRAF2 increased basal and insulin stimulated NF-kappaB activities ... Along with its protective effect, overexpressed TRAF2 increased basal and insulin stimulated NF-kappaB activities ... All effects were inhibited by IkappaB-alpha ( A32/36 ), suggesting that an amplification loop involving TRAF2 activation of NF-kappaB is implicated in insulin antiapoptotic signaling ... We also show that insulin increased manganese-superoxide dismutase ( Mn-SOD ) mRNA expression through NF-kappaB activation and that Mn-SOD contributed to insulin antiapoptotic signaling since expression of antisense Mn-SOD RNA decreased this process
Hu et al., J Biol Chem 1999 (Breast Neoplasms) : TRAIL-R1 and TRAIL-R2 can induce apoptosis of cancer cells and activate the transcription factor NF-kappaB ... TRAIL-R4 can activate NF-kappaB and protect cells from TRAIL induced apoptosis ... Here we show that TRAIL-R1-, TRAIL-R2-, and TRAIL-R4 induced NF-kappaB activation are mediated by a TRAF2-NIK-IkappaB kinase alpha/beta signaling cascade but is MEKK1 independent ... These findings suggest that TRAIL receptors induce apoptosis, NF-kappaB and JNK activation through distinct signaling pathways, and activation of NF-kappaB is not sufficient for protecting cells from TRAIL induced apoptosis
Jaspers et al., J Biol Chem 1999 : IL-8 expression is regulated at the transcriptional level in part by the transcription factor nuclear factor (NF)-kappaB
Xia et al., Mol Cell Biol 1999 (Inflammation) : RelB , a member of this family, has been characterized as a transcription activator and is involved in the constitutive NF-kappaB activity in lymphoid tissues
Whitehead et al., Mol Cell Biol 1999 (Cell Transformation, Neoplastic) : Finally, we have observed that Dbl and Dbs regulated transcription from the cyclin D1 promoter in a NF-kappaB dependent manner
Rackley et al., J Urol 1999 : In normal human urothelial cells, activation of the NF-kappaB complex in response to stimulation with TNF-alpha , LPS, and dsRNA was detected by immunohistochemical methods and EMSA ... In normal human urothelial cells, activation of the NF-kappaB complex in response to stimulation with TNF-alpha, LPS , and dsRNA was detected by immunohistochemical methods and EMSA
Takashiba et al., Infect Immun 1999 : Furthermore, stimulation with LPS induced the translocation of NF-kappaB from the cytoplasm into the nucleus
Chen et al., J Biol Chem 1999 : Stimulation of cells with LPS or Bt ( 2 ) cAMP for periods of 10 min to 24 h caused nuclear factor-kappaB (NF-kappaB) activation in the nuclei, as shown by detection of NF-kappaB-specific DNA-protein binding ... The PKA inhibitor, H8, inhibited the NF-kappaB activation induced by 6- or 12-h treatment with LPS but not that induced after 1, 3, or 24 h ... The cyclooxygenase-2 (COX-2) inhibitors, NS-398 and indomethacin, attenuated LPS induced NO release, iNOS expression, and NF-kappaB DNA-protein complex formation ... The cyclooxygenase-2 (COX-2) inhibitors, NS-398 and indomethacin, attenuated LPS induced NO release, iNOS expression, and NF-kappaB DNA-protein complex formation
Köhler et al., Vet Immunol Immunopathol 1999 : Involvement of reactive oxygen species in TNF-alpha mediated activation of the transcription factor NF-kappaB in canine dermal fibroblasts ... With respect to a possible therapeutic modulation of TNF-alpha mediated activation of Nuclear Factor-kappa B (NF-kappaB) in canine cutaneous inflammation, we investigated the role of NF-kappaB and the involvement of reactive oxygen species ( ROS ) in the TNF-alpha signalling pathway in dermal fibroblasts of the dog ... Peroxides, therefore, possibly play an important part in the redox-sensitive pathway of TNF-alpha dependent NF-kappaB activation in canine skin
Andoh et al., Clin Exp Immunol 1999 : The EMSAs indicated that sodium butyrate suppressed TNF-alpha induced nuclear factor (NF)-kappaB- and activation protein (AP)-1-DNA binding activity, but enhanced TNF-alpha induced activation of CCAAT/enhancer binding protein ( C/EBP)beta (NF-IL-6 ) -DNA binding activity
Sen et al., Eur J Immunol 1999 : Prior cross linking of CD5 also restores a normal calcium mobilization response as well as NF-kappaB activation in B-1 cells
Ishikawa et al., J Am Soc Nephrol 1999 : These results suggest that : ( 1 ) quercetin has the ability to attenuate activation of NF-kappaB ; and ( 2 ) it inhibits IL-1 triggered MCP-1 expression via suppression of NF-kappaB, but not AP-1, in glomerular cells
Rippe et al., DNA Cell Biol 1999 (Fibrosis...) : Fibrosis is frequently associated with inflammation, which is accompanied by increased levels of tumor necrosis factor-alpha (TNFalpha) and activation of the transcription factor NF-kappaB
Schottelius et al., J Biol Chem 1999 : Conflicting data exists on the effects of IL-10 on TNF- and LPS induced NF-kappaB activity in human monocytes and the molecular mechanisms involved have not been elucidated
Delgado et al., J Biol Chem 1999 : VIP and PACAP inhibit IL-12 ( p40 ) gene expression by affecting both NF-kappaB binding and the composition of the Ets-2 binding complex
Kelly et al., Virology 1999 : High levels of CAT activity were seen with HIV-LTR derived reporters that contained kappaB and TAR elements in response to transfected Tat in the absence of either transfected Rel A or exogenous TNF-alpha, and overexpression of IkappaBalpha with Tat inhibited CAT activity by 60 % to 80 %, suggesting that some activation of NF-kappaB by Tat was occurring
Irie et al., FEBS Lett 1999 : Functional interaction of Fas associated phosphatase-1 (FAP-1) with p75 ( NTR ) and their effect on NF-kappaB activation ... The results revealed that TRAF6 mediated NF-kappaB activation was suppressed by p75 ( NTR ) and that the p75 ( NTR ) -mediated NF-kappaB suppression was reduced by FAP-1 expression ... The results revealed that TRAF6 mediated NF-kappaB activation was suppressed by p75 ( NTR ) and that the p75 ( NTR ) -mediated NF-kappaB suppression was reduced by FAP-1 expression ... The results revealed that TRAF6 mediated NF-kappaB activation was suppressed by p75 ( NTR ) and that the p75 ( NTR ) -mediated NF-kappaB suppression was reduced by FAP-1 expression
Carpentier et al., FEBS Lett 1999 : TRAF1 is a TNF inducible regulator of NF-kappaB activation ... Interestingly, overexpression of TRAF1 in HEK293T completely prevented NF-kappaB activation induced by TNF, IL-1, or overexpression of TRAF2 or TRAF6 ... Interestingly, overexpression of TRAF1 in HEK293T completely prevented NF-kappaB activation induced by TNF, IL-1, or overexpression of TRAF2 or TRAF6 ... Interestingly, overexpression of TRAF1 in HEK293T completely prevented NF-kappaB activation induced by TNF , IL-1, or overexpression of TRAF2 or TRAF6 ... Interestingly, overexpression of TRAF1 in HEK293T completely prevented NF-kappaB activation induced by TNF, IL-1, or overexpression of TRAF2 or TRAF6 ... Interestingly, overexpression of TRAF1 in HEK293T completely prevented NF-kappaB activation induced by TNF, IL-1 , or overexpression of TRAF2 or TRAF6
Ali et al., FEBS Lett 1999 (Bone Resorption) : Using electrophoretic mobility shift assays, we found that not only tumour necrosis factor-alpha (TNF-alpha) but also interleukin-1beta and parathyroid hormone (PTH) caused dose and time related increases in nuclear factor kappaB (NF-kappaB)-DNA binding in Saos-2 human osteoblastic ( hOB ) cells ... Using electrophoretic mobility shift assays, we found that not only tumour necrosis factor-alpha (TNF-alpha) but also interleukin-1beta and parathyroid hormone (PTH) caused dose and time related increases in nuclear factor kappaB (NF-kappaB)-DNA binding in Saos-2 human osteoblastic ( hOB ) cells ... Using electrophoretic mobility shift assays, we found that not only tumour necrosis factor-alpha (TNF-alpha) but also interleukin-1beta and parathyroid hormone (PTH) caused dose and time related increases in nuclear factor kappaB (NF-kappaB)-DNA binding in Saos-2 human osteoblastic ( hOB ) cells ... Activation of NF-kappaB by TNF-alpha was reproduced in primary hOBs
Mandrekar et al., Int Immunol 1999 : We show here for the first time that alcohol inhibits lipopolysaccharide (LPS) induced NFkappaB activation in human monocytes by decreasing DNA binding of the p65/p50 heterodimer as seen in electrophoretic mobility shift and supershift assays ... We show here for the first time that alcohol inhibits lipopolysaccharide (LPS) induced NFkappaB activation in human monocytes by decreasing DNA binding of the p65/p50 heterodimer as seen in electrophoretic mobility shift and supershift assays ... Finally, for the first time we show that de novo protein synthesis is necessary to bring about the ethanol mediated inhibition of LPS induced NFkappaB activation
Varga et al., Int Immunol 1999 : Interruption of TCR- or IL-1R stimulated ERK cascade by PD-98059, a specific inhibitor of MAP/ERK kinase (MEK), resulted in partial suppression of nuclear factor of activated T cells activation and in complete inhibition of IL-1 stimulated NFkappaB activation
Jefferson et al., J Immunol 1999 (Calcium Signaling) : NF-kappa B inhibitors blocked the ability of toxin A to induce IL-8 secretion, and supershift analysis indicated that the major isoform of NF-kappa B activated by the toxin is a p50-p65 heterodimer
Hehner et al., J Immunol 1999 : The antiinflammatory sesquiterpene lactone parthenolide inhibits NF-kappa B by targeting the I kappa B kinase complex
Quinlan et al., J Immunol 1999 (Calcium Signaling) : The SP-elicited intracellular Ca2+ signal was required for activation and subsequent binding of both NF-AT and NF-kappa B
Ashburner et al., Cancer Res 1999 (Ataxia Telangiectasia) : Lack of involvement of ataxia telangiectasia mutated (ATM) in regulation of nuclear factor-kappaB (NF-kappaB) in human diploid fibroblasts
Plummer et al., Oncogene 1999 (Colonic Neoplasms) : Inhibition of cyclo-oxygenase 2 expression in colon cells by the chemopreventive agent curcumin involves inhibition of NF-kappaB activation via the NIK/IKK signalling complex ... Induction of COX2 by inflammatory cytokines or hypoxia induced oxidative stress can be mediated by nuclear factor kappa B (NF-kappaB)
Izumi et al., J Virol 1999 (Cell Transformation, Viral) : LMP1 spontaneously aggregates in the plasma membrane and enables two transformation effector sites ( TES1 and TES2 ) within the 200-amino-acid cytoplasmic carboxyl terminus to constitutively engage the tumor necrosis factor receptor (TNFR) associated factors TRAF1, TRAF2, TRAF3, and TRAF5 and the TNFR associated death domain proteins TRADD and RIP, thereby activating NF-kappaB and c-Jun N-terminal kinase (JNK)
Ortego et al., Atherosclerosis 1999 (Coronary Artery Disease...) : Angiotensin II (Ang II) and tumor necrosis factor alpha (TNF-alpha) increased NF-kappaB activity in VSMC ( 2 and 5-fold, respectively ) ... Angiotensin II (Ang II) and tumor necrosis factor alpha (TNF-alpha) increased NF-kappaB activity in VSMC ( 2 and 5-fold, respectively )
Hoare et al., Am J Physiol 1999 : In contrast to NAC, the metal chelators pyrrolidine dithiocarbamate and diethyldithiocarbamate attenuated IL-1beta induced NF-kappaB activation but had no effect on intracellular sulfhydryl content
Yoshidome et al., Am J Physiol 1999 (Hepatopulmonary Syndrome...) : Interleukin-10 inhibits pulmonary NF-kappaB activation and lung injury induced by hepatic ischemia-reperfusion ... The objective of the current study was to determine whether IL-10 could suppress pulmonary NF-kappaB activation and ensuing lung injury induced by hepatic ischemia-reperfusion
Guo et al., Inflammation 1999 : Antisense IRAK-2 oligonucleotide blocks IL-1 stimulated NF-kappaB activation and ICAM-1 expression in cultured endothelial cells ... Antisense IRAK-2 ODN inhibited IL-1 induced NF-KB activation and surface expression of ICAM-1 in a concentration ( 1-4 microg ) - and time ( 5-24 h ) -dependent fashion ... Antisense IRAK-2 ODN inhibited IL-1 induced NF-KB activation and surface expression of ICAM-1 in a concentration ( 1-4 microg ) - and time ( 5-24 h ) -dependent fashion
Rojanasakul et al., Mol Cell Biochem 1999 : These results indicate that silica mediated free radical generation and NF-kappaB activation play important roles in silica induced TNFalpha gene expression
Kang et al., Immunol Lett 1999 : Our results show that TPCK and TLCK inhibit NFkappaB mediated IL-12 production in macrophages
Park et al., Nephron 1999 : Decrease in NF-kappaB activity and activation of p53 induced by inhibition of interaction with ECM play roles in anoikis in SV-40 transformed collecting duct cells
Mizutani et al., J Nutr Sci Vitaminol (Tokyo) 1999 : The inductive mechanism of ascorbate on the iNOS gene was studied by examining the degradation of I kappa B alpha by Western blotting, activation of the nuclear factor kappa B (NF-kappa B) by gel shift assays, and protein levels of interferon regulatory factor 1 (IRF-1) in LPS- and IFN-gamma activated cells ... The inductive mechanism of ascorbate on the iNOS gene was studied by examining the degradation of I kappa B alpha by Western blotting, activation of the nuclear factor kappa B (NF-kappa B) by gel shift assays, and protein levels of interferon regulatory factor 1 (IRF-1) in LPS- and IFN-gamma activated cells ... Ascorbate could modulate NF-kappa B DNA binding activity in response to combined LPS and IFN-gamma activation, which increases NO production in activated macrophages ... Ascorbate could modulate NF-kappa B DNA binding activity in response to combined LPS and IFN-gamma activation, which increases NO production in activated macrophages
Kumar et al., FEBS Lett 1999 : PTPase inhibitors also blocked HIV-tat induced nuclear transcription factor NF-kappaB activation and IkappaBalpha degradation required for MMP-9 induction ... These results suggest that HIV-tat induces MMP-9 in human monocytes through activation of PTPase and NF-kappaB ... These results suggest that HIV-tat induces MMP-9 in human monocytes through activation of PTPase and NF-kappaB
Miyagawa et al., Gene 1999 : Overexpression of AZ2 inhibited TNF alpha mediated NFkappaB activation ... Overexpression of AZ2 inhibited TNF alpha mediated NFkappaB activation
Kehlen et al., J Neuroimmunol 1999 (Glioblastoma) : Activation of NF-kappaB involves the degradation of its cytoplasmatic inhibitor IkappaB-alpha , which allows the nuclear translocation of NF-kappaB, and ensures transcriptional activation of genes including IkappaB-alpha itself
Johnson et al., EMBO J 1999 : The potent transcriptional activities of Rel/NF-kappaB proteins are regulated in the cytoplasm and nucleus by the inhibitor, IkappaBalpha ... We find TNFalpha can activate the nuclear IkappaBalpha-NF-kappaB complexes by the classical mechanism of proteasome mediated degradation of IkappaBalpha
Pomerantz et al., EMBO J 1999 : TANK is a TRAF binding protein that can inhibit the binding of TRAFs to receptor tails and can also inhibit NF-kappaB activation by these receptors ... However, TANK also displays the ability to stimulate TRAF mediated NF-kappaB activation ... Kinase-inactive TBK1 inhibits TANK mediated NF-kappaB activation but does not block the activation mediated by TNF-alpha, IL-1 or CD40 ... Kinase-inactive TBK1 inhibits TANK mediated NF-kappaB activation but does not block the activation mediated by TNF-alpha, IL-1 or CD40 ... The TBK1-TANK-TRAF2 signaling complex functions upstream of NIK and the IKK complex and represents an alternative to the receptor signaling complex for TRAF mediated activation of NF-kappaB
Hay et al., Philos Trans R Soc Lond B Biol Sci 1999 : An autoregulatory loop is established when NF-kappa B induces expression of the I kappa B alpha gene and newly synthesized I kappa B alpha accumulates in the nucleus where it negatively regulates NF-kappa B-dependent transcription ... SUMO-1 modified I kappa B alpha remains associated with NF-kappa B and thus overexpression of SUMO-1 inhibits the signal induced activation of NF-kappa B-dependent transcription
Mayet et al., Eur J Clin Invest 1999 (Wegener Granulomatosis) : In addition, we established that the activation of NF-kappaB is critically involved in anti-PR-3 antibody induced endothelial IL-8 production
Chen et al., J Biol Chem 1999 : The present study investigated the involvement of the transcription factor NF-kappaB in the expression of an anti-apoptotic gene, bcl-x , using a murine macrophage cell line and peritoneal macrophages from both wild type ( p50 ( +/+ ) ) and NF-kappaB p50 gene knockout ( p50 ( -/- ) ) mice
Delfino et al., J Biol Chem 1999 : Overexpression of NF-kappaB proteins in primary Sertoli and NIH 3T3 fibroblast cells induced the CREB promoter in transient transfection assays ... Stimulation of Sertoli cells with tumor necrosis factor-alpha , an NF-kappaB activating cytokine produced by round spermatids located adjacent to Sertoli cells, stimulated the elimination of IkappaB, the translocation of additional NF-kappaB to the nucleus, and increased NF-kappaB binding to CREB promoter kappaB enhancer elements ... These data demonstrate that NF-kappaB contributes to the up-regulation of CREB expression in Sertoli cells and raises the possibility that NF-kappaB may induce other Sertoli genes required for spermatogenesis
Liang et al., J Clin Invest 1999 (Cardiomegaly) : p38 MAPK increased hBNP promoter activity through activation of the transcription factor NF-kappaB ... Thus, induction of the hBNP promoter by mechanical strain depends, at least in part, on stimulation of p38 and subsequent activation of NF-kappaB
Yan et al., Arterioscler Thromb Vasc Biol 1999 : Both iNOS and ICAM-1 are transcriptionally regulated by nuclear factor kappaB (NF-kappaB) ... Both iNOS and ICAM-1 are transcriptionally regulated by nuclear factor kappaB (NF-kappaB)
Gibson et al., Mol Cell Biol 2000 : Inhibition of NF-kappaB activation by expression of kinase-inactive MEK kinase 1 ( MEKK1 ) or dominant negative IkappaB ( DeltaIkappaB ) blocked the increase in DR4 and DR5 expression following etoposide treatment
Takuma et al., Eur J Neurosci 1999 (Reperfusion Injury) : These findings indicate that Ca2 + reperfusion induced apoptosis is mediated at least partly by ROS production and ROS cause NF-kappaB activation in cultured astrocytes
Roland et al., DNA Cell Biol 1999 : The synthetic CD4 exocyclic CDR3.AME ( 82-89 ) inhibits NF-kappaB nuclear translocation, HIV-1 promoter activation, and viral gene expression
Nonaka et al., J Neurotrauma 1999 (Brain Injuries) : Activation of transcription factor , nuclear factor kappa B (NF-kappaB) , has been shown to play a key role in inflammatory response, neuronal survival and signaling
Horie et al., Am J Pathol 1999 (Leukemia, Lymphoid...) : CD30v can activate the nuclear factor-kappaB (NF-kappaB) as CD30, and its overexpression in HL-60 induced a differentiated phenotype
Tsukamoto et al., Am J Physiol 1999 (Disease Models, Animal...) : NF-kappaB activation induced by lipopolysaccharide (LPS) in cultured hepatic macrophages ( HM ) may be abrogated by pretreatment of cells with a lipophilic iron chelator, 1,2-dimethyl-3-hydroxypyrid-4-one ( L1, deferiprone ), suggesting a role for iron in this molecular event [ M. Lin, M., R. A. Rippe, O. Niemelä, G. Brittenham, and H. Tsukamoto, Am. J. Physiol. 272 ( Gastrointest ... Treatment caused a 40 % increase in nonheme iron concentration and accentuated LPS induced NF-kappaB activation twofold
Baccam et al., Eur J Immunol 1999 : However, the carboxy-terminal 32 amino acids are not, although these residues are required for CD40 mediated NF-kappaB activation ... Thus, CD40 mediated IL-6 induction is independent of NF-kappaB activation and the binding of TRAF2 and -3, but CD40 must be engaged by trimeric CD154 on cell membranes to activate production of IL-6
Hamann et al., Eur J Immunol 2000 : Binding of lipopolysaccharide (LPS) to CHO cells does not correlate with LPS induced NF-kappaB activation
Liptay et al., Br J Pharmacol 1999 : 4. In contrast, 5ASA and sulfapyridine neither inhibit NF-kappaB/Rel activation nor induce apoptosis in T-lymphocytes at doses up to 5.0 mM ... 5. These results demonstrate that sulfasalazine, but not 5ASA or sulfapyridine, strongly inhibits NF-kappaB activation and potently induces apoptosis in T-lymphocytes
Sun et al., Oncogene 1999 : As reviewed here, Tax activates NF-kappaB primarily via a pathway leading to the chronic phosphorylation and degradation of IkappaBalpha , a cytoplasmic inhibitor of NF-kappaB ... As reviewed here, Tax activates NF-kappaB primarily via a pathway leading to the chronic phosphorylation and degradation of IkappaBalpha, a cytoplasmic inhibitor of NF-kappaB
Cahir McFarland et al., Oncogene 1999 (Cell Transformation, Viral) : Thus, EBV mediated growth transformation is genetically and biochemically linked to LMP1 mediated activation of NF-kappaB
Diamond et al., Infect Immun 2000 : Gel mobility shift assays indicate that LPS induces NF-kappaB binding activity in the nuclei of these cells, while NF IL-6 binding activity is constitutively present
Nagaki et al., J Hepatol 1999 : GalN treatment failed to affect TNF-alpha induced NF-kappaB activation
Myers et al., FEBS Lett 1999 (Calcium Signaling) : We have shown that RANK is highly expressed in mature osteoclasts and that its stimulation by RANKL results in activation of NF-kappaB and calcium signalling
Thomas et al., Transplantation 1999 : Day of transplant T cell depletion with anti-CD3 immunotoxin or F ( Ab ) 2 immunotoxin induces stable tolerance to renal allografts in rhesus monkeys given 15-deoxyspergualin (DSG) , a NF-kappaB inhibitor that suppresses proinflammatory cytokine ( PC ) production
Kovalovich et al., Hepatology 2000 (Drug-Induced Liver Injury...) : Following acute carbon tetrachloride ( CCl ( 4 ) ) treatment, we found that IL-6-/- mice developed increased hepatocellular injury and defective regeneration with significant blunting of signal transducer-and-activator of transcription protein 3 ( STAT3 ) and nuclear factor-kappaB (NF-kappaB) activation and reduced hepatocyte DNA synthetic and mitotic responses
Darville et al., Endocrinology 2000 : NF-kappaB is required for cytokine induced manganese superoxide dismutase expression in insulin producing cells
Schanstra et al., Immunopharmacology 1999 : It has been shown that B1-receptor induction during inflammation involves interleukin-1beta (IL-1beta) production and activation of nuclear factor-kappaB (NF-kappaB)
Abe et al., Am J Respir Cell Mol Biol 2000 : In accord with promoter analyses, an electrophoretic mobility shift assay showed that CAM repressed AP-1 binding in TNF-alpha treated BET-1A cells ; however, TNF-alpha induced both AP-1 and NF-kappaB binding activities in BET-1A cells
McCarty et al., Med Hypotheses 1999 (Alzheimer Disease) : NO has an autocrine anti-inflammatory impact on endothelium, owing in part to antagonism of NF-kappaB activity ; since induction of IL-6 is dependent on NF-kappaB , this may explain recent evidence that NO inhibits macrophage IL-6 production
Lee et al., J Biol Chem 2000 : Incubation of macrophages with PAO1 led to NF-kappaB dependent expression of inducible nitric-oxide synthase, COX-2, and tumor necrosis factor-alpha , which was unaffected by inhibition of Rac1 or Cdc42 function ... Incubation of macrophages with PAO1 led to NF-kappaB dependent expression of inducible nitric-oxide synthase, COX-2 , and tumor necrosis factor-alpha, which was unaffected by inhibition of Rac1 or Cdc42 function ... Incubation of macrophages with PAO1 led to NF-kappaB dependent expression of inducible nitric-oxide synthase , COX-2, and tumor necrosis factor-alpha, which was unaffected by inhibition of Rac1 or Cdc42 function ... Incubation of macrophages with PAO1 led to NF-kappaB dependent expression of inducible nitric-oxide synthase, COX-2 , and tumor necrosis factor-alpha, which was unaffected by inhibition of Rac1 or Cdc42 function
Leonardi et al., J Biol Chem 2000 : TRAF2 is required for TNF induced activation of c-Jun N-terminal kinase/stress activated protein kinase ( JNK/SAPK ), and TRAF2 can also mediate activation of NF-kappaB ... Overexpressed Filamin inhibits TRAF2 induced activation of JNK/SAPK and of NF-kappaB
Lin et al., Mol Pharmacol 2000 : The PKC inhibitors ( Go 6976 and Ro 31-8220 ) and NF-kappaB inhibitor, pyrrolidine dithiocarbamate, also attenuated IL-1beta induced PGE ( 2 ) release and COX-2 expression ... IL-1beta caused the translocation of p65 NF-kappaB from cytosol to the nucleus as well as the degradation of IkappaB-alpha in cytosol ... These results indicate that in human pulmonary epithelial cells, IL-1beta might activate phosphatidylcholine-phospholipase C through an upstream tyrosine phosphorylation to elicit PKC activation, which in turn initiates NF-kappaB activation, and finally induces COX-2 expression and PGE ( 2 ) release
D'Acquisto et al., Naunyn Schmiedebergs Arch Pharmacol 1999 (Disease Models, Animal...) : We studied the involvement of nuclear factor-kappaB (NF-kappaB) in the regulation of inducible nitric oxide synthase expression in carrageenin induced rat pleurisy ... These results suggest that in carrageenin induced rat pleurisy the activation of NF-kappaB plays a key role in inducible NO synthase protein expression and in the development of inflammatory response
Kuo et al., Am J Respir Crit Care Med 2000 (Tuberculosis, Pulmonary) : Inhibition of NF-kappaB by pyrrolidine dithiocarbamate attenuated IL-1beta and TNF-alpha synthesis ... Inhibition of NF-kappaB by pyrrolidine dithiocarbamate attenuated IL-1beta and TNF-alpha synthesis
Gewirtz et al., J Clin Invest 2000 : IL-8 secretion induced by both the prototypical proinflammatory cytokine TNF-alpha and S. typhimurium was NF-kappaB dependent ... However, NF-kappaB activation and IL-8 secretion induced by S. typhimurium, but not by TNF-alpha , was preceded by and required an increase in intracellular [ Ca ( 2+ ) ]
Erickson et al., J Virol 2000 : Although lyLMP-1 itself has negligible effects on NF-kappaB activity, it inhibits NF-kappaB activation by LMP-1 in a dose dependent manner
Jalukar et al., J Immunol 2000 : Interestingly, however, although we could show TRAF6 dependent CD40 mediated activation of NF-kappaB in 293 kidney epithelial cells, no such effect was seen in B cells, suggesting that TRAF6 has cell-type-specific functions
Sica et al., J Immunol 2000 (Fibrosarcoma...) : Autocrine production of IL-10 mediates defective IL-12 production and NF-kappa B activation in tumor associated macrophages
Cario et al., J Immunol 2000 : Downstream, LPS activated NF-kappaB in IEC in a time-, dose-, and serum dependent manner
Hoshi et al., J Biol Chem 2000 : Thrombin receptor activating peptide ( TRAP ) and basic fibroblast growth factor (bFGF) stimulated SMC proliferation and rapidly enhanced the NF-kappaB transcriptional activity in a dose dependent manner ... In contrast, although tumor necrosis factor (TNF)-alpha activated NF-kappaB transcription, E5510 had no effect on TNF-alpha induced activation ... NF-kappaB was activated after the stimulation of TRAP, bFGF, and TNF-alpha in electrophoretic mobility shift assay, and E5510 suppressed the NF-kappaB activation induced by TRAP and bFGF but not the activation by TNF-alpha ... NF-kappaB was activated after the stimulation of TRAP, bFGF, and TNF-alpha in electrophoretic mobility shift assay, and E5510 suppressed the NF-kappaB activation induced by TRAP and bFGF but not the activation by TNF-alpha ... NF-kappaB was activated after the stimulation of TRAP, bFGF, and TNF-alpha in electrophoretic mobility shift assay, and E5510 suppressed the NF-kappaB activation induced by TRAP and bFGF but not the activation by TNF-alpha ... Western blot analysis of I-kappaBalpha and I-kappaBbeta, inhibitors of NF-kappaB , indicated that I-kappaBalpha degradation, rather than I-kappaBbeta degradation, was important in NF-kappaB activation after the stimulation of TRAP and bFGF ... Western blot analysis of I-kappaBalpha and I-kappaBbeta , inhibitors of NF-kappaB , indicated that I-kappaBalpha degradation, rather than I-kappaBbeta degradation, was important in NF-kappaB activation after the stimulation of TRAP and bFGF ... These results indicate that TRAP and bFGF induced I-kappaB degradation and NF-kappaB activation through a distinct pathway from TNF-alpha and that ERK1/2 may play an important role in NF-kappaB activation induced by TRAP and bFGF ... These results indicate that TRAP and bFGF induced I-kappaB degradation and NF-kappaB activation through a distinct pathway from TNF-alpha and that ERK1/2 may play an important role in NF-kappaB activation induced by TRAP and bFGF ... These results indicate that TRAP and bFGF induced I-kappaB degradation and NF-kappaB activation through a distinct pathway from TNF-alpha and that ERK1/2 may play an important role in NF-kappaB activation induced by TRAP and bFGF
Pimentel-Muiños et al., Immunity 1999 : We show that in T cells, TNFR2 ( TNFRSF1B ) signaling is dramatically affected by the intracellular mediator RIP, a protein Ser/Thr kinase required for NF-kappaB activation by TNFR1 ( TNFRSF1A ) ... In the presence of RIP, TNFR2 triggers cell death, whereas in the absence of RIP, TNFR2 activates NF-kappaB
Nomura et al., Nihon Yakurigaku Zasshi 1999 (Glioma...) : To elucidate the intracellular mechanism of NF-kappa B activation, we performed the involvement of I kappa B alpha of NF-kappa B in the expression of inducible NO synthase (iNOS) and chemokine ( CINC ) following pretreatment with bacterial endotoxin ( LPS ) or IL-1 beta, respectively, using rat C6 glioma cells ... We found that herbimycin A, a tyrosine protein kinase inhibitor, blocked : 1 ) LPS/IFN gamma induced iNOS expression, 2 ) LPS induced intranuclear translocation of activated NF-kappa B ( p50 ... We also found in IL-1 beta induced CINC expression using cultured C6 glioma cells, the transient translocation of NF-kappa B in response to IL-1 beta is partly dependent on transient proteasome activation
Slim et al., Toxicological sciences : an official journal of the Society of Toxicology 1999 : Furthermore, PCB mediated induction of NF-kappaB and production of IL-6 were only partially blocked by alpha-NF
Yamamoto et al., FEBS Lett 2000 (Bone Resorption) : Reporter gene experiments suggested that humulon blocked the cyclooxygenase-2 expression mediated by NFkappaB and NF-IL6, but the intracellular glucocorticoid receptor was not involved
Parikh et al., Shock 2000 (Adenocarcinoma...) : IL-1beta induction of NF-kappaB activation in human intestinal epithelial cells is independent of oxyradical signaling ... Similarly, scavenging of free radicals and oxidants by pyrrolidine dithiocarbamate and dimethyl sulfoxide did not block IL-1beta induced IkappaBalpha degradation and NF-kappaB activation ... Genistein, a nonspecific tyrosine kinase inhibitor, also had no effect on IL-1beta mediated effects on NF-kappaB
Rossi et al., Nature 2000 : As IKKbeta is responsible for the activation of NF-kappaB by pro-inflammatory stimuli, our findings explain how cyclopentenone prostaglandins function and can be used to improve the utility of COX2 inhibitors
Miyakoshi et al., Br J Cancer 2000 (Glioma) : The activation and regulation of NF-kappaB are tightly controlled by IkappaB-alpha , a cellular inhibitory protein of NF-kappaB
Lee et al., J Immunol 2000 : STAT-1alpha and NF-kappaB activation are involved in IFN-gamma- or TNF-alpha mediated Fas up-regulation in microglia, respectively ... STAT-1alpha and NF-kappaB activation are involved in IFN-gamma- or TNF-alpha mediated Fas up-regulation in microglia, respectively
Reddy et al., J Immunol 2000 : Phosphatidylinositol 3-kinase as a mediator of TNF induced NF-kappa B activation ... The activation of transcription factor NF-kappa B by TNF involves the stimulation of a novel signaling cascade ... In this paper we show that phosphatidylinositol 3-kinase ( PI 3-kinase ) may play a pivotal role in TNF mediated activation of NF-kappa B-dependent genes ... A dominant negative mutant of the p85 regulatory subunit of PI 3-kinase, which is a potent inhibitor of PI 3-kinase signaling, effectively blocked the TNF induced expression of an NF-kappa B-dependent reporter gene ... Although PI 3-kinase may be required for NF-kappa B activation, overexpression of its p110 catalytic subunit alone was unable to induce an NF-kappa B/chloramphenicol acetyltransferase (CAT) reporter gene
Botella et al., J Immunol 2000 : Mobility shifts assays identified a specific NF-kappa B-binding element at +110/+120, whose mutation abolished the basal promoter activity of PECAM-1 and decreased NF-kappa B-dependent responses of the PECAM-1 gene promoter ... These results demonstrate that NF-kappa B can regulate the transcriptional activity of PECAM-1
Pan et al., Biochem Pharmacol 2000 : Furthermore, geraniin, 5GG, and TF-3 all blocked phosphorylation of IKB from the cytosolic fraction, inhibited nuclear factor-kappaB (NFkappaB) activity, and inhibited increases in inducible nitric oxide synthase levels in activated macrophages ... These results suggest that TF-3 may exert its anti-inflammatory and cancer chemopreventive actions by suppressing the activation of NFkappaB through inhibition of IKK activity
Lim et al., J Virol 2000 (Astrocytoma) : We postulate that a Tat mediated increase in SP1 binding activities augments the binding of AP1 and NF-kappaB , leading to synergistic activation of the MCP-1 promoter ... We postulate that a Tat mediated increase in SP1 binding activities augments the binding of AP1 and NF-kappaB , leading to synergistic activation of the MCP-1 promoter
Javelaud et al., Oncogene 2000 (Sarcoma, Ewing) : Induction of p21Waf1/Cip1 by TNFalpha requires NF-kappaB activity and antagonizes apoptosis in Ewing tumor cells ... Induction of p21Waf1/Cip1 by TNFalpha requires NF-kappaB activity and antagonizes apoptosis in Ewing tumor cells ... In fact, inhibition of NF-kappaB activation as a consequence of the overexpression of a degradation-resistant form of IkappaBalpha , IkappaBalpha ( A32/36 ), sensitized these cells to TNFalpha induced killing ... Our results therefore identify p21Waf1/Cip1 as a mediator of the antiapoptotic effect of TNFalpha induced NF-kappaB in Ewing tumor cells
Maggirwar et al., J Neurochem 2000 : Furthermore, the inhibition of endogenous NF-kappaB in NGF maintained neuronal PC12 cells led to the induction of c-Jun synthesis and a marked increase in cell death
Lim et al., J Neurochem 2000 (Glioma) : The p38 mitogen activated protein kinase inhibitor SB-203580 also suppressed the GMF activated NF-kappaB , suggesting the involvement of the p38 signal transduction cascade
Nakai et al., J Neurochem 2000 : The present results provide additional support for the view that NF-kappaB activation contributes to c-Myc and p53 induction and subsequent apoptosis in an excitotoxic model of Huntington 's disease ... The present results provide additional support for the view that NF-kappaB activation contributes to c-Myc and p53 induction and subsequent apoptosis in an excitotoxic model of Huntington 's disease
Miyazaki et al., J Cell Biol 2000 : Interleukin 1alpha (IL-1alpha) strongly induced ERK activation as well as NF-kappaB activation
Zamanian-Daryoush et al., Mol Cell Biol 2000 : NF-kappaB activation by double-stranded-RNA activated protein kinase ( PKR ) is mediated through NF-kappaB inducing kinase and IkappaB kinase ... Taken together, these results demonstrate that PKR dependent dsRNA induction of NF-kappaB is mediated by NIK and IKK activation ... Taken together, these results demonstrate that PKR dependent dsRNA induction of NF-kappaB is mediated by NIK and IKK activation
Bitzer et al., Genes Dev 2000 : Following stimulation with bacterial lipopolysaccharide (LPS), or the proinflammatory cytokines TNF-alpha and interleukin-1beta ( IL-1beta, NF-kappaB/RelA induces Smad7 synthesis through activation of Smad7 gene transcription ... These results suggest a mechanism of suppression of TGF-beta/SMAD signaling by opposing stimuli mediated through the activation of inhibitory Smad7 by NF-kappaB/RelA
Bandyopadhyay et al., J Immunol 2000 : Because we have previously shown that the dsRNA activated kinase PKR mediates dsRNA induction of NF-kappaB , we used murine aortic endothelial ( MuAE ) cells isolated from wild-type and PKR-null mice to investigate the role of PKR in the induction of E-selectin expression by dsRNA ( pIC ) and TNF-alpha
Furman et al., J Immunol 2000 (Leukemia, Lymphocytic, Chronic, B-Cell) : In nonmalignant B cells, CD40 ligation results in nuclear translocation and activation of NF-kappaB proteins ... In each case examined, CD40 ligation further augmented NF-kappaB activity and prolonged CLL cell survival in vitro
Kühn et al., J Immunol 2000 : However, when the IL-1 beta dependent induction of NF-kappa B was inhibited, the antiapoptotic effect of IL-1 beta was partially reversed, suggesting that NF-kappa B-mediated gene activation is part of the protective mechanism
Mondal et al., Am J Respir Cell Mol Biol 2000 : However, neither NF-kappaB translocation nor IL-8 gene induction occurred in adhered or ROFA exposed AMs
Madrid et al., Mol Cell Biol 2000 : Oncogenic Ras, an upstream activator of Akt , requires NF-kappaB to initiate transformation, at least partially through the ability of NF-kappaB to suppress transformation associated apoptosis ... In this study, we show that oncogenic H-Ras requires PI3K and Akt to stimulate the transcriptional activity of NF-kappaB ... In this study, we show that oncogenic H-Ras requires PI3K and Akt to stimulate the transcriptional activity of NF-kappaB ... In this study, we show that oncogenic H-Ras requires PI3K and Akt to stimulate the transcriptional activity of NF-kappaB ... Our work demonstrates that, unlike activated Ras, which can stimulate parallel pathways to activate both DNA binding and the transcriptional activity of NF-kappaB, Akt stimulates NF-kappaB predominantly by upregulating of the transactivation potential of p65
Li-Weber et al., Eur J Immunol 2000 : We show that both elements bind to NF-kappaB and SP-1 transcription factors and NF-kappaB is involved in transactivation of the CD95L promoter upon T cell activation ... We also show that ectopic expression of Tat protein in Jurkat T cells greatly increases NF-kappaB binding to its target DNA
Chapman et al., J Biol Chem 2000 : In this study we demonstrate that the early growth response transcription factor Egr-1 , whose DNA binding domain shares a high degree of homology with that of Sp1, can also interact with RelA in vitro and regulate NF-kappaB transcriptional activity in vivo
Yan et al., J Biol Chem 2000 : Pharmacological inhibitors of NF-kappaB blocked the induction of COX-2 protein and the stimulation of COX-2 promoter activity by B [ a ] P. Taken together, these data are likely to be important for understanding the atherogenic effects of tobacco smoke
Hayashi et al., J Biol Chem 2000 : Both DNA binding assays and luciferase assays revealed that TNF-alpha induced NF-kappaB activation is defective in T2 cells
Du et al., Cell Immunol 2000 : SEB induced expression of the IL-12/p40 gene involves activation and nuclear translocation of nuclear factor kappaB (NF-kappaB) ... SEB induced expression of the IL-12/p40 gene involves activation and nuclear translocation of nuclear factor kappaB (NF-kappaB) ... Inhibition of PKC and PKA activation results in suppression of activation and translocation of NF-kappaB
Irie et al., FEBS Lett 2000 : Stimulation of monocytes/macrophages with lipopolysaccharide (LPS) results in activation of nuclear factor-kappaB (NF-kappaB) , which plays crucial roles in regulating expression of many genes involved in the subsequent inflammatory responses ... A kinase negative mutant of TAK1 inhibited the LPS induced NF-kappaB activation both in a macrophage-like cell line, RAW 264.7, and in human embryonic kidney 293 cells expressing toll-like receptor 2 or 4 ... A kinase negative mutant of TAK1 inhibited the LPS induced NF-kappaB activation both in a macrophage-like cell line, RAW 264.7, and in human embryonic kidney 293 cells expressing toll-like receptor 2 or 4
Taher et al., Biochemistry 2000 : On the other hand, TNF-alpha is a strong activator of NF-kappa B whereas sorbitol is not ... Inhibition of NF-kappa B activation by the protease inhibitor N-alpha-tosyl-L-phenylalanine chloromethyl ketone ( TPCK ) prevents UV activation of HIV gene expression, but does not inhibit p38 MAP kinase activation
Cooper et al., Biochem Pharmacol 2000 : We then investigated the effects of transfection of monocytes with these oligonucleotides on interleukin-1beta (IL-1beta) stimulated IL-8 production, IL-8 mRNA expression, and NF-kappaB binding activity ... This single stranded oligonucleotide also inhibited IL-1beta induced translocation of NF-kappaB to the nucleus and reduced IL-8 mRNA expression
Ye et al., Ann Clin Lab Sci 2000 : Exogenous superoxide dismutase ( SOD ) enhanced the NF-kappaB activation by PMA, while catalase blocked it ... Exogenous superoxide dismutase ( SOD ) enhanced the NF-kappaB activation by PMA, while catalase blocked it
Castro et al., Phytochemistry 2000 : NF-kappa B DNA binding was inhibited at micromolar concentrations by all Sls
Wang et al., J Urol 2000 (Cystitis) : In addition, we determine the effect of lactacystin, a proteosome inhibitor, on LPS induced IkappaB degradation and NF-kappaB translocation, NK-1 receptor fluorescence intensity, and bladder inflammation
Wadleigh et al., J Biol Chem 2000 : Inhibition of endotoxin induced NF-kappaB activation by expression of an inhibitor-kappaB alpha mutant does not block endotoxin dependent COX-2 reporter activity
Martinon et al., FEBS Lett 2000 : We show that the serine/threonine kinase RIP that is required for TNF induced NF-kappaB activation is processed by caspase-8 into a dominant negative ( DN ) fragment during death receptor induced apoptosis, thereby leading to a blockade of NF-kappaB mediated anti-apoptotic signals
De Plaen et al., Br J Pharmacol 2000 : 1. We examined the effect of lipopolysaccharide (LPS) , a cell wall constituent of Gram negative bacteria, on nuclear factor kappaB (NF-kappaB) activation in the intestine and the roles of endogenous platelet activating factor (PAF), tumour necrosis factor-alpha (TNF) and neutrophils
Shao et al., Oncogene 2000 (Adenocarcinoma...) : Overexpression of the wild-type p53 gene inhibits NF-kappaB activity and synergizes with aspirin to induce apoptosis in human colon cancer cells ... In this study, to better understand the mechanism responsible for the p53 mediated apoptosis, the effect of wild-type p53 ( wt-p53 ) gene transfer on nuclear expression of NF-kappaB was determined in human colon cancer cell lines ... A Western blot analysis of nuclear extracts demonstrated that NF-kappaB protein levels in the nuclei were suppressed by the transient expression of the wt-p53 in a dose dependent manner
Kameoka et al., Biochem J 2000 (Leukemia L1210) : Taken together, these results strongly suggest that PARP is involved in the regulation of NF-kappaB through the protein modification
Ichiyama et al., Brain Res 2000 : Sodium valproate inhibits production of TNF-alpha and IL-6 and activation of NF-kappaB ... Sodium valproate inhibits production of TNF-alpha and IL-6 and activation of NF-kappaB ... Electrophoretic mobility shift assays ( EMSA ) of nuclear extracts demonstrated that VPA inhibits NF-kappaB activation induced by lipopolysaccharide (LPS) , but the other AEDs do not
Mulligan et al., Am J Pathol 2000 (Acute Disease...) : The in vivo suppressive effects of SLPI mutants on lung vascular permeability, neutrophil recruitment, and NF-kappaB activation appear to be most closely related to their trypsin inhibiting activity ... These data suggest that the suppressive effects of SLPI on the intrapulmonary activation of NF-kappaB and neutrophil recruitment into the lung may be linked to their antiprotease activity, directed, perhaps, at the intracellular proteases
Jo et al., Oncogene 2000 (Cell Transformation, Neoplastic) : In this study, inhibition of NF-kappa B activity by forced expression of a super-repressor form of I kappa B alpha , the major inhibitor of NF-kappa B , markedly decreased the growth rate, saturation density and tumorigenicity of oncogenic H-Ras transformed rat embryo fibroblasts
Ivanov et al., Oncogene 2000 (Melanoma) : Comparing early- and late-stage melanoma cells revealed low expression of TRAF2 and GCK in early-stage melanoma, which coincided with poor resistance to UV-induced, TNF mediated apoptosis ; forced expression of GCK alone or in combination with TRAF2 efficiently increased JNK and NF-kappaB activities, which coincided with increased protection against apoptosis ... Conversely, forced expression of the dominant negative form of TRAF2 or GCK in late-stage melanoma cells reduced NF-kappaB activity and decreased Fas expression, resulting in a lower degree of UV-induced, Fas mediated cell death ... Conversely, forced expression of the dominant negative form of TRAF2 or GCK in late-stage melanoma cells reduced NF-kappaB activity and decreased Fas expression, resulting in a lower degree of UV-induced, Fas mediated cell death
Ruetten et al., Mediators Inflamm 1999 : Upregulation of ICAM-1 expression on J774.2 macrophages by endotoxin involves activation of NF-kappaB but not protein tyrosine kinase : comparison to induction of iNOS ... The upregulation of ICAM-1 expression on J774.2 macrophages caused by LPS was significantly inhibited by pretreatment of the cells with inhibitors of the activation of the nuclear transcription factor NF-kappaB , such as L-1-tosylamido-2-phenylethylchloromethyl ketone ( TPCK ), pyrrolidine dithiocarbamate ( PDTC ), rotenone or calpain inhibitor I, but not by the tyrosine kinase inhibitors, tyrphostin AG126 or genistein ... Thus, the increase in the expression of ICAM-1 on J774.2 macrophages by endotoxin involves the activation of NFkappaB , but not of protein tyrosine kinase
Mazor et al., Cytokine 2000 : Because activation of NF-kappaB is involved in the regulation of the chemokine interleukin 8 (IL-8) , we hypothesized that the sesquiterpene lactones, isohelenin and parthenolide, would inhibit IL-8 gene expression in cultured human respiratory epithelium ... Pretreatment with either compound inhibited TNF-alpha mediated activation of the IL-8 promoter and TNF-alpha mediated nuclear translocation of NF-kappaB ... Stabilization of cytoplasmic I-kappaBalpha leads to inhibition of NF-kappaB nuclear translocation and of subsequent IL-8 promoter activation
Andoh et al., Pancreas 2000 (Pancreatic Neoplasms) : In PANC-1 cells, IL-1beta and TNF-alpha induced a rapid activation of nuclear factor (NF)-kappaB , and TGF-beta1 enhanced this activation slightly ... In PANC-1 cells, IL-1beta and TNF-alpha induced a rapid activation of nuclear factor (NF)-kappaB , and TGF-beta1 enhanced this activation slightly ... In PANC-1 cells, IL-1beta and TNF-alpha induced a rapid activation of nuclear factor (NF)-kappaB , and TGF-beta1 enhanced this activation slightly ... In PANC-1 cells, IL-1beta and TNF-alpha induced a rapid activation of nuclear factor (NF)-kappaB , and TGF-beta1 enhanced this activation slightly
Gentry et al., J Biol Chem 2000 (Neurilemmoma) : We investigated the function of p75 mediated NF-kappaB stimulation in regulating cell survival in the rat schwannoma cell line RN22, which expresses p75, but not TrkA ... Gel shift assays demonstrated activation of NF-kappaB in response to NGF within 30 min and lasting at least 4 h. NGF also stimulated JNK in the cells ( detected by in vitro kinase assays ) with a similar time course ... These results suggest that NGF activation of NF-kappaB through the p75 receptor promotes survival, counterbalancing the pro-apoptotic signal ... These results suggest that NGF activation of NF-kappaB through the p75 receptor promotes survival, counterbalancing the pro-apoptotic signal
Lee et al., Toxicol Lett 2000 : Additionally, to identify the mechanism of action of AAF during B-cell development, we determined the effects of AAF on LPS induced nuclear factor-kappaB (NF-kappaB) activation in 70Z/3 murine pre-B cells, CH12 murine mature B cells and S194 murine plasmacytoma cells ... LPS induced NF-kappaB activation, which is dependent on PKC, was inhibited by pretreatment with AAF for 2 h in the nuclei of 70Z/3 murine pre-B cells by detection of NF-kappaB specific DNA-protein binding
Kojima et al., Oncogene 2000 (Adenocarcinoma...) : These results indicate that LPS may stimulate DNA synthesis in certain colon carcinoma cells as a result of PGE2 production involving increased COX-2 expression that might result in turn from activation of NF-kappa B by LPS
Coudronniere et al., Proc Natl Acad Sci U S A 2000 : NF-kappa B activation induced by T cell receptor/CD28 costimulation is mediated by protein kinase C-theta ... NF-kappa B activation induced by T cell receptor/CD28 costimulation is mediated by protein kinase C-theta ... Fourth, CD3/CD28 induced CD28RE/AP-1 activation and NF-kappaB nuclear translocation were blocked by a selective PKCtheta inhibitor
Ho et al., Free Radic Biol Med 2000 (Diabetes Mellitus, Experimental...) : PBN pretreatment inhibited both alloxan- and STZ induced activation of NFkappaB and nitric oxide production
Hirahashi et al., Kidney Int 2000 : TNF-alpha induced NF-kappa B activation was blocked by overexpression of I kappa B delta N. Overexpression of I kappa B delta N potentiated TNF-alpha induced apoptosis compared to mock transfection, and the potentiation was abolished by treatment with a caspase-3 inhibitor, Z-DEVD-FMK
Mori et al., Infect Immun 2000 (Stomach Neoplasms) : Activation of intercellular adhesion molecule 1 expression by Helicobacter pylori is regulated by NF-kappaB in gastric epithelial cancer cells ... H. pylori induced the degradation of IkappaB-alpha , a major cytoplasmic inhibitor of NF-kappaB , and stimulated the expression of IkappaB-alpha mRNA ... These results show that H. pylori directly induces expression of ICAM-1 on gastric epithelial cells in an NF-kappaB dependent manner that may support leukocyte attachment during inflammation
Gil et al., Oncogene 2000 : Activation of NF-kappa B by the dsRNA dependent protein kinase, PKR involves the I kappa B kinase complex ... We have recently described that induction of NF-kappa B by PKR is involved in apoptosis commitment ... Expression by VV recombinants of IKK1 or IKK2 dominant negative mutants together with PKR showed inhibition of PKR induced NF-kappa B activation, as measured both by gel shift and luciferase reporter assays
Harhaj et al., Oncogene 2000 : Somatic mutagenesis studies of NF-kappa B signaling in human T cells : evidence for an essential role of IKK gamma in NF-kappa B activation by T-cell costimulatory signals and HTLV-I Tax protein ... Activation of NF-kappa B by both T-cell costimulatory signals and the HTLV Tax protein involves stimulation of I kappa B kinase (IKK) ... Expression of exogenous IKK gamma in the mutant cells restored NF-kappa B activation by both the T-cell costimulation agents and Tax
Karin et al., Semin Immunol 2000 (Inflammation) : Surprisingly, however, of the two catalytic subunits, only IKK beta is essential for NF-kappa B activation in response to proinflammatory stimuli
Akashi et al., J Immunol 2000 : Mouse MD-2 expression in TLR4 expressing cells enhanced LPS induced NF-kappaB activation, which was clearly inhibited by MTS510
Hattori et al., Cardiovasc Res 2000 (Diabetic Angiopathies) : High glucose or mannitol also enhanced TNFalpha stimulated NF-kappaB activity ... An antioxidant N-acetyl-L-cysteine and a selective protein kinase C ( PKC ) inhibitor GF109203X significantly suppressed the TNFalpha induced NF-kappaB activation, and abrogated potentiation of TNFalpha induced NF-kappaB activity caused by high glucose ( 27.5 mmol/l )
Jin et al., Arch Pharm Res 2000 : Inhibition of LPS induced NO production and NF-kappaB activation by a sesquiterpene from Saussurea lappa
Sohur et al., Cancer Res 2000 : In the progenitor B lymphocyte model FL5.12, whereas NF-kappaB has an antiapoptotic function in response to tumor necrosis factor-alpha , cytokine withdrawal causes nuclear translocation of NF-kappaB/cRel, where it is apoptogenic
Lavrovsky et al., Mech Ageing Dev 2000 : Age dependent increase of heme oxygenase-1 gene expression in the liver mediated by NFkappaB
Takaishi et al., J Biochem 2000 : In the present study, we examined the effects of an overexpression of IkappaBalpha , a specific natural inhibitor of NF-kappaB , on CINC-1 production
Goodman et al., J Leukoc Biol 2000 : The antioxidant pyrrolidine dithiocarbamate inhibited LPS induced nuclear factor-kappaB (NF-kappaB) activation and TSG-14 expression in macrophages
McKenzie et al., Mol Cell Biol 2000 : A mutant, catalytically inactive form of IKKalpha blocked IKKalpha-, IKKalpha-DeltaH-, and IKKalpha-DeltaLH mediated NF-kappaB activation
Chen et al., Mol Cell Biol 2000 : The expression of bcl-x suppressed apoptosis in the presence or absence of NF-kappaB activity
Lin et al., Mol Cell Biol 2000 : Protein kinase C-theta participates in NF-kappaB activation induced by CD3-CD28 costimulation through selective activation of IkappaB kinase beta ... Conversely, expression of a kinase-deficient mutant or antisense PKC-theta selectively inhibits CD3-CD28 costimulation , but not tumor necrosis factor alpha induced activation of NF-kappaB in Jurkat T cells
Huang et al., J Biol Chem 2000 : In addition, CPT activation of NF-kappaB involves degradation of cytoplasmic IkappaBalpha by the ubiquitin-proteasome pathway in a manner that depends on the IkappaB kinase complex
Ye et al., J Biol Chem 2000 : FIP3 has also been shown to repress basal and tumor necrosis factor (TNF) alpha induced NF-kappaB activity as well as to induce cell death when overexpressed
Ricca et al., Int J Cancer 2000 (Breast Neoplasms) : bcl-2 over-expression enhances NF-kappaB activity and induces mmp-9 transcription in human MCF7 ( ADR ) breast-cancer cells
Bonetti et al., J Neuropathol Exp Neurol 2000 (Neurilemmoma...) : By immunocytochemical and functional assays, we found that SC expressed TNF receptors and that TNFalpha promoted in SC cultures transient activation of transcription factors NFkappaB and c-jun in the absence of apoptosis
Vos et al., FASEB J 2000 : Here we demonstrate that cationic liposomes containing phosphorothioated oligodeoxynucleotides ( ODN ) with the kappaB binding site serving as competitive binding decoy, can prevent TNF-alpha induced NFkappaB activity in endothelial cells in vitro
Harwood et al., J Biol Chem 2000 (Colonic Neoplasms) : Regulation of FasL by NF-kappaB and AP-1 in Fas dependent thymineless death of human colon carcinoma cells ... These results demonstrate the crucial roles for NF-kappaB and AP-1 in the regulation of FasL in Fas mediated thymineless death of colon carcinoma cells
Mohan et al., J Biol Chem 2000 (Neovascularization, Pathologic) : Using cultured corneal cells, we show that FGF-2 stimulates DNA binding activity of transcription factor AP-1 but not NF-kappaB and that AP-1 stimulation is inhibited by curcuminoids
Lewis et al., J Biol Chem 2000 : The death domain kinase, receptor interacting protein ( RIP ), is one of the major components of the tumor necrosis factor receptor 1 (TNFR1) complex and plays an essential role in tumor necrosis factor (TNF) mediated nuclear factor kappaB (NF-kappaB) activation ... Here we report that RIP is a novel Hsp90 associated kinase and that disruption of Hsp90 function by its specific inhibitor, geldanamycin ( GA ), selectively causes RIP degradation and the subsequent inhibition of TNF mediated IkappaB kinase and NF-kappaB activation ... These data indicate that Hsp90 plays an important role in TNF mediated NF-kappaB activation by modulating the stability and solubility of RIP ... These data indicate that Hsp90 plays an important role in TNF mediated NF-kappaB activation by modulating the stability and solubility of RIP
Chang et al., Am J Respir Cell Mol Biol 2000 : Taking these data together, a novel mechanism is proposed in which ATRA activates promoter activity of IL-8 gene through TRX dependent NF-kappaB activation
Sun et al., Nature 2000 : Activation of NF-kappaB by tumour-necrosis factor alpha and interleukin-1 was unaffected in the mutant mice
Sanz et al., EMBO J 2000 : The atypical protein kinase C ( aPKC ) -interacting protein, p62, has previously been shown to interact with RIP, linking these kinases to NF-kappaB activation by tumor necrosis factor alpha (TNFalpha) ... Here we demonstrate that the inhibition of the aPKCs or the down-regulation of p62 severely abrogates NF-kappaB activation by IL-1 and TRAF6 , suggesting that both proteins are critical intermediaries in this pathway ... Here we demonstrate that the inhibition of the aPKCs or the down-regulation of p62 severely abrogates NF-kappaB activation by IL-1 and TRAF6, suggesting that both proteins are critical intermediaries in this pathway
Kim et al., J Biol Chem 2000 : Taken together with previous observations, these results implicate that, for the assembly of an IFN-beta enhanceosome, MEKK1 can induce IRF3 and ATF2/c-JUN through the JNK pathway, whereas it can induce NF-kappaB through the IKK pathway ... Taken together with previous observations, these results implicate that, for the assembly of an IFN-beta enhanceosome, MEKK1 can induce IRF3 and ATF2/c-JUN through the JNK pathway, whereas it can induce NF-kappaB through the IKK pathway ... Taken together with previous observations, these results implicate that, for the assembly of an IFN-beta enhanceosome, MEKK1 can induce IRF3 and ATF2/c-JUN through the JNK pathway, whereas it can induce NF-kappaB through the IKK pathway
Courilleau et al., J Biol Chem 2000 : To investigate the role of B-ind1 in Rac1 signaling, we have constructed several deletion mutants of B-ind1 and tested their ability to affect the activation of NF-kappaB by Rac1 ... Interestingly, the fragment encoding the median region of human B-ind1 acted as a dominant negative variant to block Rac1 mediated NF-kappaB activity
Madge et al., J Biol Chem 2000 : Although Akt has been reported to activate NFkappaB, LY294002 does not prevent TNF- or IL-1 induced degradation of IkappaBalpha, beta, or epsilon, transcription of NFkappaB dependent E-selectin or ICAM-1 promoter-reporter genes, or surface expression of E-selectin or ICAM-1 in human EC ... Although Akt has been reported to activate NFkappaB, LY294002 does not prevent TNF- or IL-1 induced degradation of IkappaBalpha, beta, or epsilon, transcription of NFkappaB dependent E-selectin or ICAM-1 promoter-reporter genes, or surface expression of E-selectin or ICAM-1 in human EC
Steer et al., J Biol Chem 2000 : LPS increased nuclear c-Jun/ATF-2, NF-kappaB ( 1 ) /Rel-A, and Rel-A/C-Rel transcription factor complexes, which bound specifically to oligonucleotide sequences from the -106 to -88 base pair ( bp ) region of the promoter
Schwandner et al., J Exp Med 2000 : Here we report the requirement of tumor necrosis factor receptor associated factor ( TRAF)6 in IL-17 induced NF-kappaB and JNK activation ... Lack of TRAF6 appeared to be the sole defect responsible for the observed failure to respond to IL-17, because transient transfection of TRAF6 expression plasmid into the TRAF6-deficient cells restored IL-17 induced NF-kappaB activation in a luciferase reporter assay
Seppänen et al., Eur J Endocrinol 2000 (Cystadenocarcinoma...) : The binding activity of AP-1 was found to be stimulated by the growth inhibitory cytokines, TGF-beta1 and TNF-alpha, and the binding of NF-kappaB was stimulated by TNF-alpha
Zhang et al., Immunity 2000 : The adapter protein RIP plays a crucial role in NF-kappaB activation by TNF ... Enforced expression of NEMO in cells revealed that NEMO can both promote and block NF-kappaB activation and dramatically augments the phosphorylation of c-Jun
Hamada et al., Toxicology 2000 : However, NF-kappaB was increased at 3 h while AP-1 ( Jun B and Jun D ) and CREB were increased at 15 h. Co-stimulation of 12j cells with rMu-IFN-gamma + Be increased the levels of NF-KB in 12j cell nuclei at 3 h, and the levels of AP-1 and CREB at 15 h, however, only Jun B was increased
Borgland et al., J Virol 2000 : Coinfection of REC cells with adCMVbetagal and an adenoviral vector expressing IkappaBalpha resulted in suppression of adCMVbetagal induced expression of IP-10 at 6 and 16 h, further strengthening the conclusion that adenovirus induced activation of IP-10 is dependent on NF-kappaB ... Together, these findings demonstrate that adenovirus vectors directly induce the expression of IP-10 through capsid dependent activation of NF-kappaB
Baumann et al., Proc Natl Acad Sci U S A 2000 : Raf rather synergizes with another membrane shuttle kinase MEKK1, and Raf mediated activation of NF-kappaB is blocked by a dominant negative form of MEKK1
Yin et al., Br J Haematol 2000 (Leukemia) : Forskolin inactivated NF-kappaB in K/Dau600 cells but not in K562 cl. 6 cells, whereas TNF activated NF-kappaB in K562 cl.6 cells but not in K/Dau600 cells
Bratt et al., Clin Exp Immunol 2000 : To examine the effects of AF and GSTM on TNF-alpha induced NF-kappaB activation this was measured in HUVEC nuclear extracts by an electrophoretic mobility shift assay ... To examine the effects of AF and GSTM on TNF-alpha induced NF-kappaB activation this was measured in HUVEC nuclear extracts by an electrophoretic mobility shift assay ... AF, but not GSTM, decreased TNF-alpha induced NF-kappaB activation in HUVEC
Nomura et al., Genes Cells 2000 : NF-kappaB activation through IKK-i dependent I-TRAF/TANK phosphorylation ... IKK-i significantly induced NF-kappaB activation upon over-expression, as did IKK-alpha and IKK-beta ... NF-kappaB activation by IKK-i is significantly blocked by coexpression of the N-terminal domain of I-TRAF , dominant negative TRAF2, and dominant negative NIK and IKK-beta ... NF-kappaB activation by IKK-i is significantly blocked by coexpression of the N-terminal domain of I-TRAF , dominant negative TRAF2, and dominant negative NIK and IKK-beta ... NF-kappaB activation by IKK-i is significantly blocked by coexpression of the N-terminal domain of I-TRAF, dominant negative TRAF2, and dominant negative NIK and IKK-beta ... NF-kappaB activation by IKK-i is significantly blocked by coexpression of the N-terminal domain of I-TRAF, dominant negative TRAF2, and dominant negative NIK and IKK-beta ... NF-kappaB activation by IKK-i may be mediated through phosphorylation of I-TRAF by IKK-i and subsequent liberation of TRAF2 ... These results indicate that NF-kappaB activation by IKK-i is mediated through phosphorylation of I-TRAF/TANK by IKK-i and subsequent liberation of TRAF2
van der Helm et al., Biochim Biophys Acta 2000 : The results show that the IL-1beta induced expression of group V sPLA ( 2 ) mRNA was time dependent and, similar to that of group IIA sPLA ( 2 ) mRNA, involves activation of NF-kappaB
Li et al., Arterioscler Thromb Vasc Biol 2000 : The critical role of NF-kappaB activation became evident in experiments with antisense LOX-1, which abolished ox-LDL mediated NF-kappaB activation
Mengshol et al., Arthritis Rheum 2000 (MAP Kinase Signaling System) : We found that IL-1 induction of MMP-13 requires p38 activity, c-Jun N-terminal kinase (JNK) activity and NF-kappaB translocation ... We found that IL-1 induction of MMP-13 requires p38 activity, c-Jun N-terminal kinase (JNK) activity and NF-kappaB translocation ... These results suggest that both NF-kappaB and activator protein 1 transcription factors are necessary for IL-1 induction of MMP-13 ... These results suggest that both NF-kappaB and activator protein 1 transcription factors are necessary for IL-1 induction of MMP-13 ... In chondrosarcoma cells, MMP-1 induction depends on p38 and MEK ( an MAPK kinase of the extracellular signal regulated kinase pathway ) and does not require JNK or NF-kappaB ... In chondrosarcoma cells, MMP-1 induction depends on p38 and MEK ( an MAPK kinase of the extracellular signal regulated kinase pathway ) and does not require JNK or NF-kappaB ... These studies demonstrate, for the first time, that p38, JNK, and NF-kappaB are required for IL-1 induction of MMP-13 ... These studies demonstrate, for the first time, that p38, JNK, and NF-kappaB are required for IL-1 induction of MMP-13
Remacle-Bonnet et al., Cancer Res 2000 (Adenocarcinoma...) : These findings indicate that the antiapoptotic function of IGF-I in HT29-D4 cells is based on the enhancement of the survival pathways initiated by TNF, but not Fas, and mediated by MAPK/p38, MAPK/ERK, and NF-kappaB , which act in concert to suppress the proapoptotic signals
Rudolph et al., Genes Dev 2000 (Fetal Death) : NEMO/IKKgamma-deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF-kappaB DNA binding activity in response to TNFalpha , IL-1, LPS, and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha ... NEMO/IKKgamma-deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF-kappaB DNA binding activity in response to TNFalpha, IL-1 , LPS, and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha ... NEMO/IKKgamma-deficient primary murine embryonic fibroblasts ( MEFs ) lack detectable NF-kappaB DNA binding activity in response to TNFalpha, IL-1, LPS , and Poly ( IC ) and do not show stimulus dependent IkappaB kinase activity, which correlates with a lack of phosphorylation and degradation of IkappaBalpha
Zapata et al., J Biol Chem 2000 : The Drosophila tumor necrosis factor receptor associated factor-1 ( DTRAF1 ) interacts with Pelle and regulates NFkappaB activity ... In contrast, TRAF2 mediated NFkappaB induction was partially inhibited by DTRAF1 ... In contrast, TRAF2 mediated NFkappaB induction was partially inhibited by DTRAF1 ... Mutants of DTRAF1 lacking the N-terminal region inhibited NFkappaB induction by either TRAF2 or TRAF6 ... Mutants of DTRAF1 lacking the N-terminal region inhibited NFkappaB induction by either TRAF2 or TRAF6
Smith et al., J Gen Virol 2000 : The ectromelia virus protein was found to block NF-kappaB activation and induction of IFN-gamma in response to IL-18
Ghassemi et al., AIDS Res Hum Retroviruses 2000 : In addition, transient transfection of U937 cells with full-length wild-type as well as NF-kappaB binding site deleted mutant HIV-1 LTR-CAT constructs revealed that MAC induced HIV-LTR CAT is NF-kappaB dependent
Ahmad et al., Arch Biochem Biophys 2000 : EGCG treatment was found to result in a dose based differential inhibition of TNF-alpha- and LPS mediated activation of NF-kappaB in these cells
Iber et al., Arch Biochem Biophys 2000 : Suppression of CYP2C11 gene transcription by interleukin-1 mediated by NF-kappaB binding at the transcription start site
Haddad et al., Biochem Biophys Res Commun 2000 : Sulfasalazine, a potent and specific inhibitor of NF-kappaB , induced Bax at the expense of Bcl-2, in a p53 dependent manner ... Sulfasalazine, a potent and specific inhibitor of NF-kappaB , induced Bax at the expense of Bcl-2, in a p53 dependent manner
Pise-Masison et al., Mol Cell Biol 2000 : Analysis of Tax mutants demonstrated that Tax inactivation of p53 function correlates with the ability of Tax to induce NF-kappaB but not p300 binding or CREB transactivation ... Analysis of Tax mutants demonstrated that Tax inactivation of p53 function correlates with the ability of Tax to induce NF-kappaB but not p300 binding or CREB transactivation
Zheng et al., J Immunol 2000 : Transduced Bcl-2 did not reduce TNF mediated NF-kappaB activation or constitutive expression of class I MHC molecules ... Transduced Bcl-2 did not reduce TNF mediated NF-kappaB activation or constitutive expression of class I MHC molecules
Kim et al., J Surg Res 2000 : ( i ) Hypoosmolar WE medium phosphorylated p38, ERK 1 and 2, and SAPK/JNK by 5 min. ( ii ) Hypoosmolar WE medium activated NF-kappaB at 60 min. ( iii ) HGF phosphorylated all three MAPKs and activated NF-kappaB with profiles similar to those of hypoosmotic stress ... ( i ) Both hypoosmotic cell swelling and HGF phosphorylate p38, ERK 1 and 2, and SAPK/JNK, and ( ii ) HGF, but not hypoosmotic stress, activates NF-kappaB via p38 and ERK 1 and 2 phosphorylation
Kim et al., FEBS Lett 2000 : To directly define the role of phospholipase Cgamma1 ( PLCgamma1 ) in NF-kappaB activation, NF-kappaB promoted luciferase reporter gene plasmid ( pNF-kappaB-Luc ) was transfected into rat-3Y1 fibroblasts that overexpress whole PLCgamma1 ( PLCgamma1-3Y1 ), src homology domains SH2-SH2-SH3 of PLCgamma1 ( SH223-3Y1 ) and v-src ( Src-3Y1 ) ... This study shows a direct evidence that the SH2-SH2-SH3 region of PLCgamma1 contributes to the NF-kappaB signaling and that MAPK , but not PI3K and PKC, is involved in SH2-SH2-SH3 mediated NF-kappaB activation in these cells ... This study shows a direct evidence that the SH2-SH2-SH3 region of PLCgamma1 contributes to the NF-kappaB signaling and that MAPK, but not PI3K and PKC, is involved in SH2-SH2-SH3 mediated NF-kappaB activation in these cells
Gamper et al., Mol Immunol 2000 : Over-expression of p62 ( aa 336-522 ) induces NF-kappaB activation in resting cells and augments CD40 induced NF-kappaB activation, but has no effect on control STAT1 reporter activity, either at baseline or after IFN-gamma induction
Ku et al., Cell Signal 2000 (Lymphoma, B-Cell) : Because IkappaB-alpha plays a pivotal role in the regulation of Rel/NF-kappaB activity, we have characterized both the nature and kinetics of the expression of IkappaB-alpha following anti-Ig induced apoptosis in WEHI-231 cells
Kim et al., Carcinogenesis 2000 (Cell Transformation, Neoplastic...) : Ectopic expression of the transactivating p65 subunit of NF-kappaB in MCF-10F cells induced the c-myc oncogene promoter, which is driven by two NF-kappaB elements, and endogenous c-Myc levels
Tojima et al., Nature 2000 : NAK induces IkappaB degradation and NF-kappaB activity through IKKbeta ... Endogenous NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kappaB by protein kinase C-epsilon ( PKCepsilon )
Kitahara et al., Biol Pharm Bull 2000 : TNF induced activation of transcription factor nuclear factor-kappaB (NF-kappaB) was observed in all 3 cell lines ... Pretreatment with the known potent NF-kappaB inhibitor pyrolidine dithiocarbamate ( PDTC ) profoundly suppressed the activation of NF-kappaB induced by TNF and potentiated TNF cytotoxicity in all 3 cell lines
Ryan et al., Nature 2000 : Here we show that induction of p53 causes an activation of NF-kappaB that correlates with the ability of p53 to induce apoptosis ... Activation of NF-kappaB by p53 was distinct from that mediated by tumour-necrosis factor-alpha and involved MEK1 and the activation of pp90rsk ... Activation of NF-kappaB by p53 was distinct from that mediated by tumour-necrosis factor-alpha and involved MEK1 and the activation of pp90rsk ... Inhibition of MEK1 blocked activation of NF-kappaB by p53 and completely abrogated p53 induced cell death ... Inhibition of MEK1 blocked activation of NF-kappaB by p53 and completely abrogated p53 induced cell death
Manna et al., J Biol Chem 2000 : Protein tyrosine kinase p56lck is required for ceramide induced but not tumor necrosis factor induced activation of NF-kappa B , AP-1, JNK, and apoptosis ... Protein tyrosine kinase p56lck is required for ceramide induced but not tumor necrosis factor induced activation of NF-kappa B , AP-1, JNK, and apoptosis ... These effects were specific to ceramide, as activation of NF-kappaB by phorbol 12-myristate 13-acetate, lipopolysaccharide, H ( 2 ) O ( 2 ), and TNF was minimally affected ... Overall our results demonstrate that p56 ( lck ) plays a critical role in the activation of NF-kappaB , AP-1, JNK, and apoptosis by ceramide but has minimal or no role in activation of these responses by TNF
Manna et al., J Biol Chem 2000 : Treatment of the CaCOV3 human ovarian cell line with hCG blocked TNF induced activation of NF-kappaB , IkappaBalpha degradation, and NF-kappaB dependent reporter gene transcription
Welters et al., Med Hypotheses 2000 : NF-kappaB is activated by IkappaBalpha , its inhibitor, which is phosphorylated and proteolytically degraded
Takizawa et al., Clin Exp Immunol 2000 : These findings suggested that DEP induced up-regulation of ICAM-1 gene, and this process might be largely dependent on oxidant mediated NF-kappaB activation and p38-MAPK pathways
Wang et al., Kidney Int 2000 : LPS increased NF-kappaB in a dose- and time dependent manner, which paralleled that of MCP-1 mRNA expression ... These studies prove that NF-kappaB is critical for LPS induced MCP-1 transcription, and AP-1 and Sp1 are essential for basal expression of MCP-1 in rat tubule cells
Teng et al., Gen Pharmacol 2000 : In conclusion, a p50 and p65 NF-kappaB heterodimer binds to a reverse-NF-kappaB site on the rat iNOS promoter and contributes to iNOS induction by IL-1beta and IFN-gamma in RASMCs
Paludan et al., Arch Virol 2000 : Herpes simplex virus type 2 infection of macrophages impairs IL-4 mediated inhibition of NO production through TNF-alpha induced activation of NF-kappaB ... IL-4 reduced virus induced TNF-alpha secretion and nuclear factor (NF)-kappaB activation significantly, but less in cells concomitantly treated with IFN-gamma ... Furthermore, neutralisation of residual TNF-alpha activity or inhibition of NF-kappaB activation largely restored the inhibitory effect of IL-4
Devin et al., Immunity 2000 : To understand the mechanism by which RIP and TRAF2 regulate TNF induced activation of the transcription factor NF-kappaB , we investigated their respective roles in TNF-R1 mediated IKK activation using both RIP-/- and TRAF2-/- fibroblasts ... To understand the mechanism by which RIP and TRAF2 regulate TNF induced activation of the transcription factor NF-kappaB , we investigated their respective roles in TNF-R1 mediated IKK activation using both RIP-/- and TRAF2-/- fibroblasts
Burow et al., Biochem Biophys Res Commun 2000 : PI3K and Akt stimulated NF-kappaB activation in a dose dependent manner, suggesting a common link between these two pathways ... PI3K and Akt stimulated NF-kappaB activation in a dose dependent manner, suggesting a common link between these two pathways ... PI3K and AKT cell survival signaling were correlated with increased TNF stimulated NF-kappaB activity in MCF-7 cells ... We demonstrate that while both TNFR1 and NIK are partially involved in Akt induced NF-kappaB stimulation, a dominant negative IkappaBalpha completely blocked Akt-NF-kappaB cross-talk ... We demonstrate that while both TNFR1 and NIK are partially involved in Akt induced NF-kappaB stimulation, a dominant negative IkappaBalpha completely blocked Akt-NF-kappaB cross-talk ... PI3K-Akt signaling activated NF-kappaB through both TNFR signaling dependent and -independent mechanisms, potentially representing a mechanism by which Akt functions to suppress apoptosis in cancer ... PI3K-Akt signaling activated NF-kappaB through both TNFR signaling dependent and -independent mechanisms, potentially representing a mechanism by which Akt functions to suppress apoptosis in cancer
Shinoura et al., Biochem Biophys Res Commun 2000 (Fibrosarcoma...) : Inhibition of NF-kappaB in the presence of tumor necrosis factor-alpha (TNF) is supposed to be a promising cancer therapeutic approach, since it disrupts the protective mechanism of NF-kappaB activated by TNF
Manna et al., J Immunol 2000 : These effects were specific to HIV-tat, as activation of NF-kappa B by PMA, LPS, H2O2, and TNF was minimally affected ... These effects were specific to HIV-tat, as activation of NF-kappa B by PMA, LPS , H2O2, and TNF was minimally affected ... Moreover, the reconstitution of JCaM1 cells with p56lck tyrosine kinase reversed the HIV-tat induced NF-kappa B activation and cytotoxicity ... Overall, our results demonstrate that p56lck plays a critical role in the activation of NF-kappa B , AP-1, JNK, and apoptosis by HIV-tat protein but has minimal or no role in activation of these responses by TNF
Jensen et al., J Immunol 2000 (Carcinoma, Hepatocellular) : The proinflammatory cytokine IL-1 induces the biosynthesis of a number of immunologically important proteins during infection, tissue damage, and/or stress, in part through the activation of the transcription factor NF-kappaB ... Overexpression of a membrane anchored fusion protein of sIL-1RAcP and MHC in HepG2 cells inhibits IL-1 mediated NF-kappaB activation, whereas coexpression of IL-1RI with membrane anchored sIL-1RAcP restores the capacity of the cells to respond to IL-1
Yoo et al., J Immunol 2000 : Although HSP induction did not affect total cellular expression of NF-kappa B, TNF-alpha induced increase in NF-kappa B-DNA binding activity and nuclear translocation of the p65 subunit of NF-kappa B were inhibited by prior HSP induction, suggesting that activation of NF-kappa B was blocked
D'Addario et al., J Mol Biol 2000 : Furthermore, we demonstrate that activation of TNF-alpha by gp350 is mediated by NF-kappaB through signal transduction pathways involving PKC, PI3-K and tyrosine kinases
Abraham et al., Crit Care Med 2000 (Respiratory Distress Syndrome, Adult) : Phosphorylation, ubiquination, and proteolysis of I kappaB allows NF-kappaB to translocate to the nucleus and induce transcription, once associated with the transcriptional cofactor CBP
Zhang et al., Br J Pharmacol 2000 : Nuclear NF-kappaB activity was robustly induced by TNF-alpha ... However, TNF-alpha alone did not induce iNOS promoter activity, protein expression, or nitrite production, indicating that NF-kappaB activation alone is not sufficient for iNOS induction
Hippenstiel et al., Blood 2000 : Inhibition of p38 MAPK by SB 202190 also blocked LPS induced NF-kappaB activation and IL-8 synthesis ... Inhibition of p38 MAPK by SB 202190 also blocked LPS induced NF-kappaB activation and IL-8 synthesis ... In summary, our data suggest that LPS induced NF-kappaB activation and IL-8 synthesis in HUVECs are regulated by both a Rho dependent signaling pathway and the MKK6/p38 kinase cascade
Mühlenbeck et al., J Biol Chem 2000 : Overexpression of the tumor necrosis factor (TNF) related apoptosis inducing ligand ( TRAIL ) receptors, TRAIL-R1 and TRAIL-R2, induces apoptosis and activation of NF-kappaB in cultured cells ... Overexpression of the tumor necrosis factor (TNF) related apoptosis inducing ligand ( TRAIL ) receptors, TRAIL-R1 and TRAIL-R2, induces apoptosis and activation of NF-kappaB in cultured cells ... Moreover, in these cell lines interleukin-6 secretion and NF-kappaB activation were induced by cross linked or non-cross linked anti-TRAIL , as well as by both receptor-specific IgGs
Millet et al., J Biol Chem 2000 : CGRP inhibits the accumulation of NF-kappaB complexes in the nucleus by preventing phosphorylation and degradation of the NF-kappaB inhibitor IkappaB
Abraham et al., J Biol Chem 2000 (Scleroderma, Systemic) : The activity of TNFalpha was blocked by NF-kappaB inhibitors
Bajpai et al., J Exp Med 2000 (Immunologic Deficiency Syndromes) : Thus, BTK is essential for activation of NF-kappaB via the B cell receptor
Petro et al., J Exp Med 2000 (Agammaglobulinemia...) : BCR stimulation leads to the activation of transcription factor nuclear factor (NF)-kappaB , which in turn regulates genes controlling B cell growth ... We now demonstrate that a null mutation in btk known to cause the xid phenotype prevents BCR induced activation of NF-kappaB
Heyen et al., Brain Res Mol Brain Res 2000 : Interleukin (IL)-10 inhibits IL-6 production in microglia by preventing activation of NF-kappaB ... The purpose of this study was to determine if interleukin (IL)-10 inhibits lipopolysaccharide (LPS) induced IL-6 production in microglia by inhibiting activation of nuclear factor-kappaB (NF-kappaB) ... Because NF-kappaB is the predominant transcription factor responsible for IL-6 transcription in response to inflammatory stimuli, it was hypothesized that IL-10 inhibited IL-6 production by preventing nuclear translocation of NF-kappaB ... Consistent with this idea, LPS increased nuclear translocation of NF-kappaB as assessed by gel mobility shift assay ... Pretreatment with IL-10, however, inhibited LPS induced activation of NF-kappaB ... Furthermore, inhibition of NF-kappaB activity with tosyl-Phe-chloromethlyketone ( a serine protease inhibitor that prevents degradation of the NF-kappaB-IkappaB complex ), completely blocked LPS induced IL-6 production ... These data suggest that IL-10 inhibited IL-6 production in microglia by decreasing the activity of NF-kappaB and, therefore, extend what little is known of the intricate relationship between anti-inflammatory and inflammatory cytokines in the central nervous system
Dieter et al., Mediators Inflamm 1999 : Inhibition of NF-kappaB inhibits the LPS- but not the MTP-PE induced release of TNF-alpha , nitric oxide and PGE2 ... Inhibition of NF-kappaB inhibits the LPS- but not the MTP-PE induced release of TNF-alpha, nitric oxide and PGE2
Manna et al., Oncogene 2000 (Lung Neoplasms) : The increase in TNF receptors sensitized H596 cells to TNF induced activation of NF-kappaB , AP-1 and apoptosis ... A549 cells, however, were completely resistant to TNF induced activation of NF-kappaB , AP-1 and apoptosis
Muenchen et al., Clin Cancer Res 2000 (Prostatic Neoplasms) : p6R-IkappaB ( S32A + S36A ) blocked the stimulation of NF-kappaB activity by TNF-alpha in prostate cancer cells
Brzoska et al., Ann N Y Acad Sci 1999 : Furthermore, we determined the effect of alpha-MSH on the IL-1 beta induced activation of the nuclear factor kappa B (NF kappa B) -- a major transcription factor for chemokine genes ... Furthermore, we determined the effect of alpha-MSH on the IL-1 beta induced activation of the nuclear factor kappa B (NF kappa B) -- a major transcription factor for chemokine genes ... Electrophoretic mobility-shift-assays showed that alpha-MSH, in a dose range from 10 ( -6 ) to 10 ( -12 ) M, significantly downregulated the IL-1 beta induced activation of NF kappa B 10 minutes after stimulation
Kalden et al., Ann N Y Acad Sci 1999 (Inflammation) : alpha-MSH significantly downregulated the LPS mediated activation of NF-kappa B , in a dose dependent manner
Böhm et al., Ann N Y Acad Sci 1999 : alpha-MSH also induces activation of NF-kappa B but does not modulate DNA binding on costimulation with IL-1 beta
Gu et al., Int J Dev Neurosci 2000 : We injected 192 IgG-saporin, an immunotoxin selectively taken up by neurotrophin receptor p75 ( NTR ) -bearing neurons, into lateral ventricles, followed by infusions of anti-NGF to assess NF-kappaB , ChAT and NGF responses to PIL after anti-NGF infusion
Shimpo et al., Hypertension 2000 : Increased nitrite production by aspirin-like drugs was accompanied by increased iNOS mRNA and protein accumulation in VSMCs. Addition of IL-1beta activated nuclear factor kappaB (NF-kappaB) in VSMCs, but sodium salicylate did not affect IL-1beta induced NF-kappaB activation
Kasof et al., FEBS Lett 2000 : These experiments further characterize RIP3 mediated apoptosis and NF-kappaB activation
Pahan et al., J Neurochem 2000 (Neuritis) : As activation of nuclear factor-kappaB (NF-kappaB) is necessary for the transcription of iNOS , we examined the effect of Deltap21 ( ras ) on the activation of NF-kappaB ... Expression of Deltap21 ( ras ) inhibited the DNA binding as well as the transcriptional activity of NF-kappaB in activated astrocytes, suggesting that Deltap21 ( ras ) inhibits the expression of iNOS by inhibiting the activation of NF-kappaB
Manna et al., J Immunol 2000 : Vesnarinone suppresses TNF induced activation of NF-kappa B , c-Jun kinase, and apoptosis ... Vesnarinone blocked TNF induced activation of NF-kappa B in a concentration- and time dependent manner ... This effect was mediated through inhibition of phosphorylation and degradation of I kappa B alpha , an inhibitor of NF-kappa B ... The effects of vesnarinone were not cell type specific, as it blocked TNF induced NF-kappa B activation in a variety of cells ... Vesnarinone also blocked NF-kappa B activation induced by several other inflammatory agents, inhibited the TNF induced activation of transcription factor AP-1 , and suppressed the TNF induced activation of c-Jun N-terminal kinase and mitogen activated protein kinase kinase ... Vesnarinone also blocked NF-kappa B activation induced by several other inflammatory agents, inhibited the TNF induced activation of transcription factor AP-1, and suppressed the TNF induced activation of c-Jun N-terminal kinase and mitogen activated protein kinase kinase
Jeon et al., J Immunol 2000 : Thiol-reactive metal compounds inhibit NF-kappa B activation by blocking I kappa B kinase
Li et al., J Immunol 2000 : NF-kappa B regulates VCAM-1 expression on fibroblast-like synoviocytes ... These results indicate that NF-kappa B mediates TNF-alpha induced VCAM-1 expression on mFLS
Haycock et al., J Biol Chem 2000 (Melanoma) : We have previously shown that alpha-melanocyte stimulating hormone ( alpha-MSH ) can oppose tumor necrosis factor alpha activation of NF-kappaB ( 1-2 h ) and intercellular adhesion molecule 1 up-regulation ( mRNA by 3 h and protein by 24 h ) in melanocytes and melanoma cells
Kajino et al., Oncogene 2000 (Pancreatic Neoplasms) : To confirm the role of NF-kappaB in blocking apoptosis, we generated stable cell lines expressing IkappaBdeltaN, a highly stable form of IkappaBalpha , a cytoplasmic inhibitor of NF-kappaB
Martin et al., J Biol Chem 2000 : Tumor necrosis factor-alpha activation of NF-kappa B requires the phosphorylation of Ser-471 in the transactivation domain of c-Rel ... Moreover, the overexpression of the mutant protein c-Rel S471N into Jurkat cells abolished TNFalpha induced NF-kappaB activity, thus demonstrating that this mutation is responsible for the failure of TNFalpha stimulation of NF-kappaB
Sekine et al., J Cell Physiol 2000 : Synergistic activation of NF-kappab and inducible isoform of nitric oxide synthase induction by interferon-gamma and tumor necrosis factor-alpha in INS-1 cells ... Synergistic activation of NF-kappab and inducible isoform of nitric oxide synthase induction by interferon-gamma and tumor necrosis factor-alpha in INS-1 cells ... TNF-alpha did not affect STAT1 activation, but stimulated DNA binding and transcriptional activity of NF-kappaB , both of which were further increased by IFN-gamma ... In conclusion, IFN-gamma activates STAT1 and potentiates TNF-alpha induced NF-kappaB activation in INS-1 cells, thereby inducing iNOS and cell destruction
Ohtsubo et al., J Cell Physiol 2000 (Carcinoma, Squamous Cell) : These observations suggest the activation of NFkappaB via IkappaB degradation and STAT1 via specific receptor kinase activity synergistically induce WAF1 gene expression in A431 cells
Allport et al., Mol Hum Reprod 2000 : NF-kappaB and AP-1 are required for cyclo-oxygenase 2 gene expression in amnion epithelial cell line ( WISH )
Stasiolek et al., J Biol Chem 2000 (Astrocytoma...) : Expression of inflammatory nitric oxide synthase ( NOS2 ) is mediated by transcription factor NFkappaB ... These results suggest that IkappaB-beta contributes to persistent NFkappaB activation and NOS2 expression in glial cells, that IkappaB-beta is a stress protein inducible by hyperthermia or proteasome inhibitors, and that delayed addition of proteasome inhibitors can have stimulatory rather than inhibitory actions
Tabata YHibi et al., Leukemia & lymphoma 2000 (Carcinoma...) : NF-kappaB assay revealed that HLH-LMP1 activated NF-kappaB significantly more than that of B95.8-LMP1 ( p=0.032 )
Kang et al., J Toxicol Environ Health 2000 : Further evidence for the involvement of ROS in NF-kappaB activation is that 1 mM H2O2 enhanced NF-kappaB/DNA binding and that this activation was inhibited by catalase ... Specific inhibitors of protein tyrosine kinase , such as herbimycin A, genistein, and AG-494, prevented NF-kappaB activation in silica treated cells
Smahi et al., Nature 2000 (Incontinentia Pigmenti) : NEMO is required for the activation of the transcription factor NF-kappaB and is therefore central to many immune, inflammatory and apoptotic pathways
Doostzadeh-Cizeron et al., J Biol Chem 2000 : Expression of p84N5 also leads to activation of NF-kappaB as indicated by nuclear translocation of p65RelA and transcriptional activation of a NF-kappaB dependent reporter promoter
Manna et al., J Immunol 2000 : Resveratrol blocked TNF induced activation of NF-kappaB in a dose- and time dependent manner ... Suppression of TNF induced NF-kappaB activation by resveratrol was not restricted to myeloid cells ( U-937 ) ; it was also observed in lymphoid ( Jurkat ) and epithelial ( HeLa and H4 ) cells ... Resveratrol also blocked NF-kappaB activation induced by PMA, LPS , H2O2, okadaic acid, and ceramide
Sasu et al., Am J Physiol Heart Circ Physiol 2000 : Because the proliferative effect of IL-1 is additive with that of serum, the present studies assessed the role of IkappaB kinases ( IKKs ) and NF-kappaB in both IL-1- and serum induced VSMC proliferation ... IL-1beta ( 1 ng/ml ) induced marked and persistent NF-kappaB activation in VSMC that was maximal at 1 h and persisted for 3 days ... Electrophoretic mobility shift assay and supershift analysis indicated that IL-1 induced NF-kappaB complexes consisted of p65/p50 heterodimers and p50 homodimers
Gupta et al., Kidney Int 2000 : To test the hypothesis that alpha-MSH inhibits the expression of inducible trans activating factors involved in NOS2 regulation, we used RAW 264.7 macrophage cells to examine the effects of alpha-MSH on the activation of nuclear factor-kappaB (NF-kappaB) and CCAAT/enhancer binding protein-beta ( C/EBPbeta ), trans acting factors known to be involved in LPS + interferon (IFN)-gamma induction of the NOS2 gene ... To test the hypothesis that alpha-MSH inhibits the expression of inducible trans activating factors involved in NOS2 regulation, we used RAW 264.7 macrophage cells to examine the effects of alpha-MSH on the activation of nuclear factor-kappaB (NF-kappaB) and CCAAT/enhancer binding protein-beta ( C/EBPbeta ), trans acting factors known to be involved in LPS + interferon (IFN)-gamma induction of the NOS2 gene ... To test the hypothesis that alpha-MSH inhibits the expression of inducible trans activating factors involved in NOS2 regulation, we used RAW 264.7 macrophage cells to examine the effects of alpha-MSH on the activation of nuclear factor-kappaB (NF-kappaB) and CCAAT/enhancer binding protein-beta ( C/EBPbeta ), trans acting factors known to be involved in LPS + interferon (IFN)-gamma induction of the NOS2 gene ... Gel shift assays were performed to identify NF-kappaB and C/EBP DNA binding activities in LPS + IFN-gamma treated RAW 264.7 cells in the presence and absence of alpha-MSH
Weidenbach et al., J Hepatol 2000 (Colitis...) : Although low levels of portal endotoxin were measured during the first 2 days of colitis, no significant NF-kappaB/Rel activity and NOS2 induction were detected in the liver
Lee et al., Mol Endocrinol 2000 (Neoplasms) : Overall, these results suggest that the nuclear receptor coactivator ASC-2 also mediates transactivations by SRF, AP-1, and NFkappaB , which may contribute to the putative, ASC-2 mediated tumorigenesis
Williams et al., Shock 2000 (Intestinal Perforation...) : Inhibition of LPS induced NFkappaB activation by a glucan ligand involves down-regulation of IKKbeta kinase activity and altered phosphorylation and degradation of IkappaBalpha ... Inhibition of LPS induced NFkappaB activation by a glucan ligand involves down-regulation of IKKbeta kinase activity and altered phosphorylation and degradation of IkappaBalpha ... Inhibition of LPS induced NFkappaB activation by a glucan ligand involves down-regulation of IKKbeta kinase activity and altered phosphorylation and degradation of IkappaBalpha ... Pre- or post-treatment with glucan phosphate decreased ( P < 0.05 ) tissue phospho-IkappaBalpha levels in CLP mice. Phospho-IkappaBalpha in the glucan-CLP group were not significantly different from the unoperated controls. To investigate mechanisms we examined IKKbeta kinase activity, IkappaBalpha phosphorylation and degradation, and NFkappaB activity in a murine macrophage cell line, J774a.1, treated with LPS ( 1 microg/mL ) and/or glucan phosphate ( 1 microg/mL ) for up to 120 min. The glucan ligand blunted LPS induced IKKbeta kinase activity, phosphorylation and degradation of IkappaBalpha, and NFkappaB nuclear binding activity. The data indicate that one mechanism by which ( 1 -- > 3 ) -beta-D-glucan may alter the response to endotoxin or polymicrobial sepsis involves modulation of IKK3 kinase activity with subsequent decreases in IkappaBalpha phosphorylation and NFkappaB activation
Wooten et al., Mol Cell Biol 2000 : Src , PI3K, and PKC-iota were likewise required for NGF induced NF-kappaB activation and cell survival, whereas Ras was not required for either survival or NF-kappaB activation but was required for differentiation ... Src, PI3K, and PKC-iota were likewise required for NGF induced NF-kappaB activation and cell survival, whereas Ras was not required for either survival or NF-kappaB activation but was required for differentiation ... Src, PI3K , and PKC-iota were likewise required for NGF induced NF-kappaB activation and cell survival, whereas Ras was not required for either survival or NF-kappaB activation but was required for differentiation ... Src, PI3K , and PKC-iota were likewise required for NGF induced NF-kappaB activation and cell survival, whereas Ras was not required for either survival or NF-kappaB activation but was required for differentiation ... Consistent with a role for Src in regulating NF-kappaB activation, an absence of Src activity impaired recruitment of PKC-iota into an IKK complex and markedly impaired NGF induced translocation of p65/NF-kappaB to the nucleus ... Consistent with a role for Src in regulating NF-kappaB activation, an absence of Src activity impaired recruitment of PKC-iota into an IKK complex and markedly impaired NGF induced translocation of p65/NF-kappaB to the nucleus
Wang et al., Mol Cell Biol 2000 : As a consequence, TNF-alpha mediated I-kappaB degradation and NF-kappaB activation were markedly enhanced in Stat1-deficient cells, whereas overexpression of Stat1 in 293T cells blocked NF-kappaB activation by TNF-alpha ... As a consequence, TNF-alpha mediated I-kappaB degradation and NF-kappaB activation were markedly enhanced in Stat1-deficient cells, whereas overexpression of Stat1 in 293T cells blocked NF-kappaB activation by TNF-alpha
Bonnet et al., Mol Cell Biol 2000 : PKR stimulates NF-kappaB irrespective of its kinase function by interacting with the IkappaB kinase complex ... Here, we have studied the ability of PKR to activate NF-kappaB in a reporter assay and have shown for the first time that two catalytically inactive PKR mutants, PKR/KR296 and a deletion mutant ( PKR/Del42 ) which lacks the potential eIF2alpha binding domain, can also activate NF-kappaB ... This result indicated that NF-kappaB activation by PKR does not require its kinase activity and that it is independent of the PKR-eIF2alpha relationship
Yamit-Hezi et al., J Biol Chem 2000 : TAF(II)105, a substoichiometric coactivator subunit of TFIID, is important for activation of anti-apoptotic genes by NF-kappaB in response to the cytokine tumor necrosis factor (TNF)-alpha
Guo et al., Inflammation 2000 : IRAK-2 and PI 3-kinase synergistically activate NF-kappaB and AP-1 ... IRAK-2 and PI 3-kinase synergistically activate NF-kappaB and AP-1 ... As a result, antisense IRAK-2 ODN or antisense p110 PI 3-kinase ODN inhibited IL-1 induced NF-kappaB and AP-1 activation in HepG2 cells ... As a result, antisense IRAK-2 ODN or antisense p110 PI 3-kinase ODN inhibited IL-1 induced NF-kappaB and AP-1 activation in HepG2 cells ... These results indicate that IRAK-2 is necessary but insufficient to activate NF-kappaB and AP-1 completely and that although PI 3-kinase is not sufficient for NF-kappaB full activation, it is sufficient to activate AP-1 completely ... The effects of IRAK-2 or PI 3-kinase on NF-kappaB and AP-1 activation were confirmed by the results that overexpression of IRAK-2 failed to fully activate NF-kappaB and AP-1 and that overexpression of p110 PI 3-kinase is insufficient for NF-kappaB full activation but sufficient for AP-1 activation ... The effects of IRAK-2 or PI 3-kinase on NF-kappaB and AP-1 activation were confirmed by the results that overexpression of IRAK-2 failed to fully activate NF-kappaB and AP-1 and that overexpression of p110 PI 3-kinase is insufficient for NF-kappaB full activation but sufficient for AP-1 activation ... Cotransfection experiments showed that the combination of antisense IRAK-2 ODN and antisense p110 PI 3-kinase ODN resulted in additive inhibition of NF-kappaB as well as AP-1 activation ... On the other hand, coexpression of IRAK-2 with p110 PI 3-kinase led to a synergistic activation of NF-kappaB and AP-1
Andoh et al., Int J Mol Med 2000 : EMSAs indicated that trichostatin A weakly suppressed TNF-alpha induced NF-kappaB and NF-IL6 activation
Yujiri et al., Proc Natl Acad Sci U S A 2000 : MEKK1 is required for JNK but not NF-kappaB activation in response to virus infection, microtubule disruption, and stimulation of embryonic stem cells with lysophosphatidic acid ... Thus, MEKK1 senses microtubule integrity, contributes to the regulation of fibroblast and epithelial cell migration, and is required for activation of JNK but not NF-kappaB in response to selected stress stimuli
Sylla et al., Proc Natl Acad Sci U S A 2000 (Epstein-Barr Virus Infections...) : The X-linked lymphoproliferative syndrome gene product SH2D1A associates with p62dok ( Dok1 ) and activates NF-kappa B ... NF-kappaB activation by SH2D1A does not depend on the wild-type SH2 domain and is inhibited by a dominant negative IkappaB kinase beta
Molestina et al., Infect Immun 2000 (Arteriosclerosis) : Requirement for NF-kappaB in transcriptional activation of monocyte chemotactic protein 1 by Chlamydia pneumoniae in human endothelial cells ... To examine the underlying mechanisms by which C. pneumoniae infection induces MCP-1 production in endothelial cells, the present study investigated the role of transcription factor NF-kappaB in MCP-1 mRNA expression
Russell et al., Am J Physiol Gastrointest Liver Physiol 2000 (Ischemia) : These findings suggest that superoxide and TNF-alpha mediate gut I/R induced E-selectin expression via an NF-kappaB dependent mechanism ; this upregulation of E-selectin contributes significantly to I/R induced neutrophil recruitment ... These findings suggest that superoxide and TNF-alpha mediate gut I/R induced E-selectin expression via an NF-kappaB dependent mechanism ; this upregulation of E-selectin contributes significantly to I/R induced neutrophil recruitment
Ryu et al., Biochem Biophys Res Commun 2000 : Acetaminophen inhibited iNOS mRNA expression, promoter activity of iNOS gene and nuclear factor-kappa B (NF-kappaB) binding activity induced by LPS plus IFN-gamma , whereas salicylates did not show any effect on them ... Acetaminophen inhibited iNOS mRNA expression, promoter activity of iNOS gene and nuclear factor-kappa B (NF-kappaB) binding activity induced by LPS plus IFN-gamma, whereas salicylates did not show any effect on them
Takaori-Kondo et al., Biochem Biophys Res Commun 2000 : TRAF3 blocked OX40-, TRAF2 induced NF-kappaB activation, but not NIK- and IKKalpha induced NF-kappaB activation, indicating that TRAF3 blocks the pathway between TRAF2 and NIK ... TRAF3 blocked OX40-, TRAF2 induced NF-kappaB activation, but not NIK- and IKKalpha induced NF-kappaB activation, indicating that TRAF3 blocks the pathway between TRAF2 and NIK ... TRAF3 blocked OX40-, TRAF2 induced NF-kappaB activation, but not NIK- and IKKalpha induced NF-kappaB activation , indicating that TRAF3 blocks the pathway between TRAF2 and NIK ... TRAF3 blocked OX40-, TRAF2 induced NF-kappaB activation, but not NIK- and IKKalpha induced NF-kappaB activation , indicating that TRAF3 blocks the pathway between TRAF2 and NIK ... C-terminal deletion mutants as well as the N-terminal deletion mutant of TRAF3 inhibited NF-kappaB activation induced by OX40 or TRAF2 ... C-terminal deletion mutants as well as the N-terminal deletion mutant of TRAF3 inhibited NF-kappaB activation induced by OX40 or TRAF2 ... Thus, it is suggested that TRAF3 actively inhibits NF-kappaB activation induced by OX40
Arch et al., Biochem Biophys Res Commun 2000 : After recruitment to a receptor, TRAFs initiate formation of multiprotein complexes that induce downstream events, such as translocation of transcription factor nuclear factor kappaB (NF-kappaB) and activation of c-Jun N-terminal kinase (JNK)
Amrani et al., Mol Pharmacol 2000 : Thapsigargin, an agent that depletes intracellular calcium stores, abrogated both cytokine mediated NF-kappaB dependent ICAM-1 mRNA transcription and protein expression but had no effect on IkappaB degradation
Vallejo et al., J Immunol 2000 (MAP Kinase Signaling System) : These results indicate that both NF-kappaB and AP-1 ( via p38 MAPK ) are involved in the regulation of TNF-alpha production in GBS stimulated neonatal monocytes
Zeng et al., J Cell Biochem 2000 : Human monocyte/neutrophil elastase inhibitor ( MNEI ) is regulated by PU.1/Spi-1, Sp1, and NF-kappaB
Dienz et al., J Biol Chem 2000 : PKCtheta induced NF-kappaB activation was synergistically augmented by Vav ... In addition to the synergistic activation cascade, Vav also triggered NF-kappaB activity on a separate route ... CD3/CD28 induced activation of NF-kappaB was inhibited by dominant negative forms of Vav or PKCtheta, revealing their essential role in this activation pathway ... CD3/CD28 induced activation of NF-kappaB was inhibited by dominant negative forms of Vav or PKCtheta, revealing their essential role in this activation pathway
Chen et al., Br J Pharmacol 2000 : Exogenous PGE ( 2 ) induced NF-kappaB activation and potentiated nitrite accumulation in response to LPS
Ruiz-Ortega et al., Circ Res 2000 : In the present work, we investigated the effect of angiotensin II (Ang II) on NF-kappaB activity in rat vascular smooth muscle cells, evaluating the molecular mechanisms and the specific receptor subtype involved ... Ang II increased NF-kappaB DNA binding ( 5-fold, 10 ( - ) ( 9 ) mol/L at 1 hour ; electrophoretic mobility shift assay ), nuclear translocation of p50/p65 subunits, and cytosolic inhibitor kappaBalpha ( IkappaBalpha ) degradation ... AT(1) ( DUP753 ) and AT(2) ( PD123319 and CGP42112 ) receptor antagonists inhibited Ang II-induced NF-kappaB DNA binding in a dose dependent manner ( approximately 85 % for each one ; 10 ( - ) ( 5 ) mol/L at 1 hour ) ... AT(1) ( DUP753 ) and AT(2) ( PD123319 and CGP42112 ) receptor antagonists inhibited Ang II-induced NF-kappaB DNA binding in a dose dependent manner ( approximately 85 % for each one ; 10 ( - ) ( 5 ) mol/L at 1 hour )
Narayanan et al., Mol Immunol 2000 (Eosinophilia...) : Stable expression of IkappaBalpha mutated at Ser32 and Ser36 to Ala caused inhibition of NF-kappaB activation, indicating that this activation involves the IkappaB kinase mediated pathway
Paik et al., J Biol Chem 2000 (Adenocarcinoma...) : This inhibition correlates with the suppression of TNFalpha- or LPS induced NFkappaB activation by flufenamic acid ... The inhibitor of extracellular signal regulated protein kinase, p38, or NFkappaB does not affect the NSAID induced COX-2 expression ... An activator of peroxisome proliferator activated receptor gamma, 15-deoxy-Delta ( 12,14 ) -prostaglandin J ( 2 ), also induces COX-2 expression and inhibits TNFalpha induced NFkappaB activation and COX-2 expression ... An activator of peroxisome proliferator activated receptor gamma, 15-deoxy-Delta ( 12,14 ) -prostaglandin J ( 2 ), also induces COX-2 expression and inhibits TNFalpha induced NFkappaB activation and COX-2 expression
Ritchie et al., J Cell Physiol 2000 (Glioblastoma) : Accordingly, ectopic over-expression of NFkappaB subunits in GBM cells elevated the levels of fibronectin gene expression, providing direct evidence for a regulatory role for NF-kappaB in ECM protein production
van't Hof et al., Proc Natl Acad Sci U S A 2000 (Bone Resorption) : Immunohistochemical studies and electrophoretic mobility shift assays performed on bone marrow cocultures from iNOS-deficient mice showed abnormalities in IL-1 induced nuclear translocation of the p65 component of NFkappaB and in NFkappaB-DNA binding, which were reversed by treatment with the NO donor S-nitroso-acetyl penicillamine ... These results show that the iNOS pathway is essential for IL-1 induced bone resorption and suggest that the effects of NO may be mediated by modulating IL-1 induced nuclear activation of NFkappaB in osteoclast precursors
Chen et al., Diabetes 2000 (Insulinoma) : However, normal IL-1beta mediated translocation of NF-kappaB and induction of inducible nitric oxide synthase expression and nitric oxide production was severely impaired in the INS-1res cell lines, suggesting a mechanism for the IL-1beta resistance
Chainy et al., Oncogene 2000 : In the present report we show that anethole is a potent inhibitor of TNF induced NF-kappaB activation ( an early response ) as monitored by electrophoretic mobility shift assay, IkappaBalpha phosphorylation and degradation, and NF-kappaB reporter gene expression ... Suppression of IkappaBalpha phosphorylation and NF-kappaB reporter gene expression induced by TRAF2 and NIK, suggests that anethole acts on IkappaBalpha kinase
Ivanov et al., Oncogene 2000 (Melanoma) : Forced expression of a dominant negative form of p38 ( p38-ASP ) or treatment with p38 pharmacological inhibitor, SB203580, increases NF-kappaB activity, Fas expression and the levels of UVC induced apoptosis in late stage melanoma cells ... Inhibition of p38 activity also restored NF-kappaB activity and Fas expression in early-phase melanoma cells, suggesting that p38 elicited suppression of Fas expression is not restricted to late phase melanoma
Jaspers et al., Am J Respir Cell Mol Biol 2000 (MAP Kinase Signaling System) : Both nuclear translocation of NF-kappaB and enhanced kappaB dependent transcription induced by V ( IV ) were inhibited by overexpression of catalase , but not Cu, Zn superoxide dismutase ( Cu, Zn-SOD ), indicating that peroxides rather than superoxides initiated signaling
Vasiliou et al., Biochem Biophys Res Commun 2000 (Fibrosis...) : Involvement of p65 in the regulation of NF-kappaB in rat hepatic stellate cells during cirrhosis ... The differential NF-kappaB binding observed in these two cell lines appears to depend on the phosphorylation of the p65 subunit rather than the expression levels of either p65 or p50
Gau et al., Toxicol Appl Pharmacol 2000 : Our results reveal that the activation of NF-kappaB by LPS is suppressed by HA in a dose- and time dependent manner ... Furthermore, HA reduces NF-kappaB binding to DNA slightly, but completely inhibits the degradation of IkappaBalpha at a concentration of 100 microg/ml
Morigiwa et al., Nihon Yakurigaku Zasshi 2000 : Release of cytokines may be induced via Ca2+ influx and activation of NFAT, NF-kappa B or p44/42 and p38 MAP kinases, switching off the mitotic signal transduction pathway and triggering the apoptotic cascade at the same time ... Release of cytokines may be induced via Ca2+ influx and activation of NFAT, NF-kappa B or p44/42 and p38 MAP kinases, switching off the mitotic signal transduction pathway and triggering the apoptotic cascade at the same time
Yang et al., J Biol Chem 2000 : LPS stimulated NF-kappaB reporter activity and IL-8 production but not Elk-1 activity in HEK-TLR4 cells ... TLR4 mediated NF-kappaB reporter activity and IL-8 production was enhanced by the expression of MD-2
Carter et al., J Biol Chem 2000 : We have shown that both the ERK and p38 mitogen activated protein ( MAP ) kinases are necessary for cytokine gene transcription and that the p38 MAP kinase is required for NF-kappaB-driven transcription, so we hypothesized that the MEK -- > ERK pathway regulated NF-kappaB-driven transcription as well ... We have shown that both the ERK and p38 mitogen activated protein ( MAP ) kinases are necessary for cytokine gene transcription and that the p38 MAP kinase is required for NF-kappaB-driven transcription, so we hypothesized that the MEK -- > ERK pathway regulated NF-kappaB-driven transcription as well ... We found that the MEK -- > ERK pathway negatively regulates NF-kappaB-driven transcription, in part, by inhibiting p38 MAP kinase activity
Camandola et al., J Neurosci Res 2000 : We now report that overexpression of full-length Par-4 in cultured PC12 cells results in a suppression of basal NF-kappaB DNA binding activity and NF-kappaB activation following trophic factor withdrawal ( TFW )
Aliprantis et al., EMBO J 2000 : Here we show that the adaptor molecule, myeloid differentiation factor 88 ( MyD88 ), mediates both apoptosis and nuclear factor-kappaB (NF-kappaB) activation by BLP stimulated TLR2
Inohara et al., J Biol Chem 2000 : Nod1 is an Apaf-1-like molecule composed of a caspase-recruitment domain ( CARD ), nucleotide binding domain, and leucine-rich repeats that associates with the CARD containing kinase RICK and activates nuclear factor kappaB (NF-kappaB) ... A mutant form of IKKgamma deficient in binding to IKKalpha and IKKbeta inhibited NF-kappaB activation induced by RICK or RIP ... Enforced oligomerization of RICK or RIP as well as of IKKgamma, IKKalpha , or IKKbeta was sufficient for induction of NF-kappaB activation ... Enforced oligomerization of RICK or RIP as well as of IKKgamma, IKKalpha, or IKKbeta was sufficient for induction of NF-kappaB activation ... Enforced oligomerization of RICK or RIP as well as of IKKgamma , IKKalpha, or IKKbeta was sufficient for induction of NF-kappaB activation
Yan et al., Nat Immunol 2000 : In vitro soluble TACI-Fc fusion protein blocked BLyS induced NF-kappa B activation in B lymphoma cells and IgM production in peripheral blood B cells
Takaya et al., Pancreas 2000 (Pancreatic Neoplasms) : IL-1beta and TNF-alpha induced a rapid activation of nuclear factor (NF)-kappaB in PANC-1 cells, and the increase in chemokine mRNA expression correlated with NF-kappaB activation ... IL-1beta and TNF-alpha induced a rapid activation of nuclear factor (NF)-kappaB in PANC-1 cells, and the increase in chemokine mRNA expression correlated with NF-kappaB activation
Takaesu et al., Mol Cell 2000 : Expression of TAB2 induces JNK and NF-kappaB activation, whereas a dominant negative mutant TAB2 impairs their activation by IL-1
Peters et al., Mol Cell 2000 : A dominant negative mutant of IKKepsilon blocks induction of NF-kappaB by both PMA and activation of the T cell receptor but has no effect on the activation of NF-KB by TNFalpha or IL-1 ... A dominant negative mutant of IKKepsilon blocks induction of NF-kappaB by both PMA and activation of the T cell receptor but has no effect on the activation of NF-KB by TNFalpha or IL-1 ... These observations indicate that the activation of NF-kappaB requires multiple distinct IkappaB kinase complexes, which respond to both overlapping and discrete signaling pathways
Becker et al., J Biol Chem 2000 : Furthermore, we demonstrate TLR mRNA in hTBE cells and NF-kappa B activation following LPS
Born et al., J Biol Chem 2000 : Neither receptor alone was able to mediate transcriptional activation of NF-kappaB in response to IL-1alpha, IL-1beta, or IL-18 ... Neither receptor alone was able to mediate transcriptional activation of NF-kappaB in response to IL-1alpha, IL-1beta , or IL-18 ... Neither receptor alone was able to mediate transcriptional activation of NF-kappaB in response to IL-1alpha, IL-1beta , or IL-18
Liu et al., J Neurosci 2000 : However, pretreatment with oATP downregulated activation of NF-kappaB and AP-1 by IL-1beta or TNFalpha ... However, pretreatment with oATP downregulated activation of NF-kappaB and AP-1 by IL-1beta or TNFalpha
Simoncini et al., Circ Res 2000 : Consistently, gel-shift assays showed that E ( 2 ) and Dex comparably inhibit LPS induced activation of NF-kappaB , whereas E ( 2 ) inhibited LPS induced activation of AP-1 and GATA to a greater extent than Dex
Xiao et al., J Biol Chem 2000 : Consistently, when expressed alone, the NRD potently inhibits NIK mediated NF-kappaB signaling
Maeda et al., Gastroenterology 2000 (Stomach Neoplasms) : H. pylori promotes degradation of IkappaBalpha , a cytoplasmic inhibitor of NF-kappaB
Pyatt et al., Cell Biol Toxicol 2000 : We have previously demonstrated that 1 micromol/L HQ inhibits TNF induced activation of NFkappaB in CD4+ T cells, resulting in decreased IL-2 production
Wang et al., Biochem Biophys Res Commun 2000 (Colonic Neoplasms) : Taken together, these results suggest that inhibition of PI3-kinase in HT-29 cells results in induction of NF-kappaB binding activity and transactivation which is independent of IkappaBalpha degradation
Rimbach et al., Mol Cell Biol Res Commun 2000 : Macrophages stimulated with IFN-gamma activate NF-kappa B and induce MCP-1 gene expression in primary human endothelial cells
Arsura et al., Mol Cell Biol 2000 (Cell Transformation, Neoplastic) : As judged by kinase assays and transfection of dominant negative IKK-1 and IKK-2 expression vectors, NF-kappaB activation by Ras appeared to be mediated by both IKK-1 and IKK-2, while Raf induced NF-kappaB activation was mediated by IKK-2 ... As judged by kinase assays and transfection of dominant negative IKK-1 and IKK-2 expression vectors, NF-kappaB activation by Ras appeared to be mediated by both IKK-1 and IKK-2, while Raf induced NF-kappaB activation was mediated by IKK-2
Peng et al., Cell Mol Life Sci 2000 : In the present study, we investigated the effect of pycnogenol, an antioxidant phytochemical, on the activation of NF-kappa B and the induction of VCAM-1 and ICAM-1 in tumor necrosis factor (TNF)-alpha treated human umbilical vein endothelial cells ( HUVECs ) ... Gel-shift analysis of HUVEC demonstrated that pretreatment with pycnogenol exhibited a concentration dependent suppression of TNF-alpha induced activation of NF-kappa B
Maellaro et al., J Cell Sci 2000 (Melanoma) : In addition, GGT expression results in an up-regulation of the transcription factor NF-kappa B , which could explain the higher metastatic behaviour reported for GGT-rich melanoma cells as compared to their GGT-poor counterparts
Farmer et al., Am J Physiol Endocrinol Metab 2000 : We tested the hypothesis that the TNF-alpha stimulated transcription factor nuclear factor (NF)-kappaB could, in part, mediate TNF-alpha action by inhibiting the transcriptional potency of the VDR and RXR at their cognate cis regulatory sites ... Electrophoretic mobility shift experiments did not reveal a direct effect of recombinant NF-kappaB or its individual subunits on the binding of heterodimeric VDR-RXR to DNA ... These results suggest that TNF-alpha inhibition of hormone stimulated transcriptional activation may be mediated by activation of NF-kappaB
Krappmann et al., J Biol Chem 2000 : Overexpression of either IKK gamma or IKAP blocked tumor necrosis factor alpha induction of an NF-kappa B-dependent reporter construct, but IKAP in addition affected several NF-kappa B-independent promoters ... Whereas specific down-regulation of IKK gamma protein levels by antisense oligonucleotides significantly reduced cytokine mediated activation of the IKK complex and subsequent NF-kappa B activation , a similar reduction of IKAP protein levels had no effect on NF-kappa B signaling
Takizawa et al., Respirology 2000 (Inflammation...) : Transient transfection of airway epithelial cells with wild and NF-kappaB binding motifs indicated that the activation of NF-kappaB was essential for IL-8 gene upregulation by reporter gene assay
Hoeflich et al., Nature 2000 (Fetal Death...) : Requirement for glycogen synthase kinase-3beta in cell survival and NF-kappaB activation ... The early steps leading to NF-kappaB activation ( degradation of I-kappaB and translocation of NF-kappaB to the nucleus ) were unaffected by the loss of GSK-3beta, indicating that NF-kappaB is regulated by GSK-3beta at the level of the transcriptional complex
Cho et al., Toxicol Appl Pharmacol 2000 : Since the expression of the iNOS gene is mainly regulated by NF-kappaB , we then examined the effects of CHL on the NF-kappaB DNA binding activity, using an electrophoretic mobility shift assay ... These results suggest that CHL suppresses the nitric oxide production and iNOS mRNA expression mediated by the inhibition of NF-kappaB activation, and its action mechanism may be based on its antioxidant effect
Loftis et al., Am J Physiol Regul Integr Comp Physiol 2000 (Anoxia...) : Here we tested the hypothesis that hypoxic suppression of postbacteremic TNF-alpha gene expression is transcriptionally mediated by reduced activation of NF-kappaB
Tamatani et al., J Neurochem 2000 : These findings suggest a pathway where NFkappaB activation induces overexpression of Bcl-2 and Bcl-x , which function to prevent apoptotic cell death following H/R treatments ... These findings suggest a pathway where NFkappaB activation induces overexpression of Bcl-2 and Bcl-x, which function to prevent apoptotic cell death following H/R treatments
Biswas et al., Proc Natl Acad Sci U S A 2000 (Breast Neoplasms) : An inhibitor of phosphatidylinositol 3 kinase, Ly294-002, blocked this event, suggesting a role of the former in the activation of NF-kappaB by EGF ... Go6976, a well characterized NF-kappaB inhibitor, blocked EGF induced NF-kappaB activation and up-regulation of cell-cycle regulatory proteins
Tummala et al., J Mol Cell Cardiol 2000 : These data suggest that flavin binding proteins such as NADH/NADPH oxidase can regulate VCAM-1 gene expression independent of NF- kappa B
Jeon et al., J Pharmacol Exp Ther 2000 (MAP Kinase Signaling System) : Our data also show that the p38 kinase pathway is specifically involved in LPS induced NF-kappaB/Rel activation and iNOS expression because NF-kappaB/Rel DNA binding and iNOS mRNA production in the presence of a specific inhibitor of p38 kinase, SB203580, were dramatically diminished ... In contrast, PD98059, a specific inhibitor of mitogen activated protein kinase/extracellular signal regulated protein kinase kinase 1 had no effect on NF-kappaB/Rel activation and iNOS expression
Legrand-Poels et al., Biochem J 2000 (Adenocarcinoma...) : Thus IL-6 promoter repression in HTM-29 cells probably results from a failure of cytokine activated NF-kappaB to exert its transactivating activities
Hatzoglou et al., J Immunol 2000 (MAP Kinase Signaling System) : We also show that overexpression of BCMA in 293 cells activates NF-kappa B , Elk-1, the c-Jun N-terminal kinase, and the p38 mitogen activated protein kinase ... Furthermore, as BCMA is lacking a `` death domain '' and its overexpression activates NF-kappa B and c-Jun N-terminal kinase, we can reasonably hypothesize that upon binding of its corresponding ligand BCMA transduces signals for cell survival and proliferation
Paludan et al., Eur Cytokine Netw 2000 (Herpes Genitalis) : The results indicate that TNF-alpha , secreted by virus infected macrophages, activates NF-kappa B which impairs the IL-13 mediated inhibition of inducible NO synthase (iNOS) expression
Hirota et al., Biochem Biophys Res Commun 2000 : Nucleoredoxin, glutaredoxin, and thioredoxin differentially regulate NF-kappaB , AP-1, and CREB activation in HEK293 cells ... Nucleoredoxin , glutaredoxin, and thioredoxin differentially regulate NF-kappaB , AP-1, and CREB activation in HEK293 cells ... Nucleoredoxin, glutaredoxin , and thioredoxin differentially regulate NF-kappaB , AP-1, and CREB activation in HEK293 cells ... We demonstrated here that intracellular localization of these redox molecules differ from each other and that the redox molecules differentially regulate NF-kappaB , AP-1, and CREB activation induced by TNFalpha , PMA, and forskolin and by expression of signaling intermediate kinases, NIK, MEKK, and PKA in HEK293 cells
Shu et al., Proc Natl Acad Sci U S A 2000 : Overexpression of BCMA activates NF-kappaB , and this activation is potentiated by TALL-1 ... Overexpression of BCMA activates NF-kappaB , and this activation is potentiated by TALL-1 ... Moreover, BCMA mediated NF-kappaB activation is inhibited by dominant negative mutants of TNF receptor associated factor 5 (TRAF5) , TRAF6, NF-kappaB inducing kinase (NIK), and IkappaB kinase (IKK) ... Moreover, BCMA mediated NF-kappaB activation is inhibited by dominant negative mutants of TNF receptor associated factor 5 (TRAF5), TRAF6 , NF-kappaB inducing kinase (NIK), and IkappaB kinase (IKK) ... Moreover, BCMA mediated NF-kappaB activation is inhibited by dominant negative mutants of TNF receptor associated factor 5 (TRAF5), TRAF6, NF-kappaB inducing kinase (NIK), and IkappaB kinase (IKK) ... Moreover, BCMA mediated NF-kappaB activation is inhibited by dominant negative mutants of TNF receptor associated factor 5 (TRAF5), TRAF6, NF-kappaB inducing kinase (NIK), and IkappaB kinase (IKK)
Tomita et al., Rheumatology (Oxford) 2000 (Arthritis, Rheumatoid) : These genes are regulated by the transcription factor NFkappaB which in turn is activated by tumour necrosis factor-alpha (TNF-alpha) and cytokines
Tomita et al., Gene Ther 2000 (Glomerulonephritis) : The expression of several cytokines and adhesion molecules is regulated by the transcription factor NFkappaB , which is activated by tumor necrosis factor alpha (TNF-alpha)
Chae et al., Endocrinology 2000 : Recently, it has been reported that tumor necrosis factor-alpha (TNF-alpha) activates the release of ceramide and that ceramide acts as a mediator for the TNF-alpha induced stimulation of the binding affinity of nuclear factor-KB (NF-KB) , a ubiquitous transcription factor of particular importance in immune and inflammatory responses ... In this study we demonstrate that dexamethasone, which reduces the production of ceramide, significantly inhibits TNF-alpha induced activation of NF-KB , c-Jun N-terminal kinase, also known as stress activating protein kinase, caspase-3-like cysteine protease, redistribution of cytochrome c, and apoptosis in MC3T3E1 osteoblasts
Manna et al., Cancer Res 2000 : Oleandrin blocked tumor necrosis factor (TNF) induced activation of NF-kappaB in a concentration- and time dependent manner ... This effect was mediated through inhibition of phosphorylation and degradation of IkappaBalpha , an inhibitor of NF-kappaB ... A proprietary hot water extract of oleander ( Anvirzel ) also blocked TNF induced NF-kappaB activation ; subsequent fractionation of the extract revealed that this activity was attributable to oleandrin ... The effects of oleandrin were not cell type specific, because it blocked TNF induced NF-kappaB activation in a variety of cells
Ingram et al., American journal of physiology. Renal physiology 2000 : Thus strain induces p38 activity and translocation to the nucleus and p38 dependent increases in nuclear protein binding to NF-kappaB
Mochida et al., Kokubyo Gakkai Zasshi 2000 (MAP Kinase Signaling System) : Here we report that ASK 1 binds to TAK 1 and dissociates TAK 1 from TNF receptor associated factor 6 ( TRAF 6 ), and inhibits TAK 1- and TRAF 6- , but not NF-kappa B-inducing kinase (NIK) induced NF-kappa B activation ... Here we report that ASK 1 binds to TAK 1 and dissociates TAK 1 from TNF receptor associated factor 6 ( TRAF 6 ), and inhibits TAK 1- and TRAF 6-, but not NF-kappa B-inducing kinase (NIK) induced NF-kappa B activation
Hajra et al., Proc Natl Acad Sci U S A 2000 (Arteriosclerosis) : In control C57BL/6 mice, expression levels of p65 , IkappaBalpha, and IkappaBbeta were 5- to 18-fold higher in the HP region, yet NF-kappaB was activated in a minority of endothelial cells ... In control C57BL/6 mice, expression levels of p65, IkappaBalpha , and IkappaBbeta were 5- to 18-fold higher in the HP region, yet NF-kappaB was activated in a minority of endothelial cells ... In control C57BL/6 mice, expression levels of p65, IkappaBalpha, and IkappaBbeta were 5- to 18-fold higher in the HP region, yet NF-kappaB was activated in a minority of endothelial cells
Chen et al., Biochem Biophys Res Commun 2000 : Here we show that TFF3 activated NF-kappaB p50/p65 heterodimer within 1 h in IEC-18 cells ( a nontransformed rat intestinal epithelial cell line ) ... Taken together, these data indicate that ( 1 ) TFF3 is an endogenous gastrointestinal peptide with anti-anoikic property ; ( 2 ) TFF3 activates NF-kappaB in enterocytes ; and ( 3 ) TFF3 induced resistance to anoikis in intestinal epithelial cells is mediated by a distinct signaling cascade linked to NF-kappaB
Sareneva et al., J Immunol 2000 : Pretreatment of T cells with IFN-alpha or IL-12 enhanced IL-18 induced NF-kappaB activation and sensitized the cells to respond to lower concentrations of IL-18
Rameshwar et al., J Immunol 2000 (Primary Myelofibrosis) : The results indicate that IL-1 is necessary for TGF-beta production in IMF monocytes, but NF-kappaB activation is required for the production of endogenous IL-1
True et al., Am J Physiol Lung Cell Mol Physiol 2000 : Challenge with H ( 2 ) O ( 2 ) also failed to induce NF-kappaB-driven reporter gene expression in the transduced HMEC-1 cells, whereas TNF-alpha increased the NF-kappaB-driven gene expression approximately 10-fold
Kamthong et al., Biochem J 2000 : Electromobility shift assays showed that IL-1alpha predominantly activated nuclear factor kappaB (NF-kappaB) , while PMA preferentially activated activator protein-1 (AP-1)
Xia et al., Biochem Pharmacol 2000 : Because the transcriptional activation of the mouse TNF-alpha gene is highly dependent on kappaB enhancers, the present study determined whether the synthetic carbocyclic nucleoside 9- [ ( 1S,3R ) -cis-cyclopentan-3-ol ] adenine ( cPA ) inhibited LPS induced nuclear factor-kappaB (NF-kappaB) activation in these cells ... However, both the LPS induced degradation of the cytoplasmic NF-kappaB inhibitor IkappaBalpha and the nuclear translocation of the NF-kappaB p50, p65, and c-Rel peptides were unaffected by treatment of the cells with the nucleoside
Schulte et al., FASEB J 2000 : Invasin induced NF-kappaB activation correlated with degradation of IkappaBalpha and the inhibition of NF-kappaB by specific inhibitors of IkappaB activation blocked invasin induced IL-8 secretion
White et al., Br J Haematol 2000 (Translocation, Genetic) : We assessed the effect of APC on LPS induced tumour necrosis factor alpha (TNF-alpha) production and on the activation of the central proinflammatory transcription factor nuclear factor-kappaB (NF-kappaB) in a THP-1 cell line ... We assessed the effect of APC on LPS induced tumour necrosis factor alpha (TNF-alpha) production and on the activation of the central proinflammatory transcription factor nuclear factor-kappaB (NF-kappaB) in a THP-1 cell line ... We assessed the effect of APC on LPS induced tumour necrosis factor alpha (TNF-alpha) production and on the activation of the central proinflammatory transcription factor nuclear factor-kappaB (NF-kappaB) in a THP-1 cell line ... APC also inhibited LPS induced activation of NF-kappaB , with APC ( 200 microg/ml ) abolishing the effect of LPS ( 100 ng/ml ) ... APC also inhibited LPS induced activation of NF-kappaB , with APC ( 200 microg/ml ) abolishing the effect of LPS ( 100 ng/ml ) ... The ability of APC to inhibit LPS induced translocation of NF-kappaB is likely to be a significant event given the critical role of the latter in the host inflammatory response ... The ability of APC to inhibit LPS induced translocation of NF-kappaB is likely to be a significant event given the critical role of the latter in the host inflammatory response
Jyothi et al., Scand J Immunol 2000 (Neoplasms, Experimental) : Induction of iNOS enhanced nuclear factor (NF)-kappaB binding activity in IL-2 activated NK cells, which was confirmed using L-NAME, an NO synthesis inhibitor
Lacey et al., Am J Pathol 2000 : In contrast, OPGL enhanced both NF-kappaB and JNK kinase activation and increased the expression of c-src, but not bcl-2 and bcl-X ( L ) mRNA
McKay et al., Mol Endocrinol 2000 : GR-mediated repression of p65 transactivation and p65 mediated repression of GR transactivation, as well as the physical interaction between NF-kappaB and GR, are enhanced by CBP
Jeon et al., Immunopharmacology 2000 : Dexamethasone inhibits IL-1 beta gene expression in LPS stimulated RAW 264.7 cells by blocking NF-kappa B/Rel and AP-1 activation ... Treatment of DEX to RAW 264.7 cells induced a dose related inhibition of NF-kappaB/Rel and AP-1 in chloramphenicol acetyltransferase activity, while neither NF-IL6 nor CREB/ATF activation was affected by DEX ... Treatment of DEX to RAW 264.7 cells induced a dose related inhibition of NF-kappaB/Rel and AP-1 in chloramphenicol acetyltransferase activity, while neither NF-IL6 nor CREB/ATF activation was affected by DEX ... Treatment of DEX to RAW 264.7 cells induced a dose related inhibition of NF-kappaB/Rel and AP-1 in chloramphenicol acetyltransferase activity, while neither NF-IL6 nor CREB/ATF activation was affected by DEX ... These results suggest that DEX may inhibit IL-1beta gene expression by a mechanism involving the blocking of LPS induced NF-kappaB/Rel and AP-1 activation ... These results suggest that DEX may inhibit IL-1beta gene expression by a mechanism involving the blocking of LPS induced NF-kappaB/Rel and AP-1 activation
Janabi et al., Arterioscler Thromb Vasc Biol 2000 (Arteriosclerosis) : Oxidized LDL induced NF-kappa B activation and subsequent expression of proinflammatory genes are defective in monocyte derived macrophages from CD36-deficient patients
Mudge et al., Exp Nephrol 2000 (Glomerulonephritis, IGA) : No significant C5b-9 induced NF-kappa B activation was detected by electrophoretic mobility shift assays, luciferase activity of RMCs transfected with a NF-kappa B-driven luciferase reporter construct, nor by Northern blots for the NF-kappa B-responsive mRNA species monocyte chemoattractant protein-1 or I kappa B alpha
Brenier-Pinchart et al., Mol Cell Biochem 2000 : We also found that MCP-1 secretion induced by live T. gondii is blocked by inhibitors of nuclear factor (NF)-kappaB activation, ALLN and MG132 ... Such data indicate that NF-kappaB could be involved in T. gondii induced MCP-1 production
Nakao et al., Mol Cell Biochem 2000 : Activation of NFkappaB is necessary for IL-1beta induced cyclooxygenase-2 (COX-2) expression in human gingival fibroblasts ... Since pro-inflammatory cytokine IL-1beta has been shown to induce prostaglandin E2 ( PGE2 ) release in human gingival fibroblasts (HGF), here we analyzed the effect of IL-1beta on the expression of COX-2 and the activation of NFkappaB in HGF ... Since pro-inflammatory cytokine IL-1beta has been shown to induce prostaglandin E2 ( PGE2 ) release in human gingival fibroblasts (HGF), here we analyzed the effect of IL-1beta on the expression of COX-2 and the activation of NFkappaB in HGF
Azuma et al., Arthritis Rheum 2000 : Because matrix metalloproteinase 9 (MMP-9) is regulated by nuclear factor kappaB (NF-kappaB) , we investigated the effect of a super-repressor form of inhibitor of nuclear factor kappaBalpha ( srIkappaBalpha ) on the suppression of TNFalpha induced MMP-9 production in acinar cells
Prasad et al., J Infect Dis 2000 (Communicable Diseases...) : Decreased production of IL-2 in zinc deficiency may be due to decreased activation of NF-kappaB and subsequent decreased gene expression of IL-2 and IL-2 receptors
Seitz et al., Cancer Res 2000 (Hyperplasia) : An active NF-kappaB subunit expressed in the epidermis of p21 ( CiP1-/- mice, however, displays only partial growth-inhibitory effects, suggesting that full NF-kappaB growth inhibition is only partially p21 ( Cip1 ) dependent in this setting
Chen et al., J Immunol 2000 : NF-kappaB DNA-protein binding and COX-2 promoter activity were enhanced by TNF-alpha , and these effects were inhibited by genistein, U73122, staurosporine, or pyrolidine dithiocarbamate ... These results suggest that, in NCI-H292 epithelial cells, TNF-alpha might activate phospholipase C-gamma2 via an upstream tyrosine kinase to induce activation of PKC-alpha and protein tyrosine kinase, resulting in the activation of NF-kappaB inducing kinase and IKK1/2 , and NF-kappaB in the COX-2 promoter, then initiation of COX-2 expression and PGE2 release
Lakics et al., J Immunol 2000 : Although the composition of NF-kappaB complexes detected by EMSA and supershift analysis in nuclear lysates derived from Bcl-xL transfectants and control cells was indistinguishable, LPS induced inhibitory kappaBalpha degradation, as well as NF-kappaB binding and AP-1 activation, were slightly decreased by ectopic expression of Bcl-xL
Udalova et al., Infect Immun 2000 : Direct evidence for involvement of NF-kappaB in transcriptional activation of tumor necrosis factor by a spirochetal lipoprotein ... B. recurrentis Vmp rapidly stimulates nuclear translocation of NF-kappaB and proinflammatory cytokine gene expression in the human monocyte-like cell line MonoMac 6 ... By overexpressing disabled mutant IkappaBalpha in MonoMac 6 cells cotransfected with a reporter gene, we provide evidence that NF-kappaB is essential for the transcriptional activation of TNF in this system
Gaeta et al., Lab Invest 2000 : Overexpressed TNFR1 ( -DD ) did not activate NF-kappaB but acted as a ligand-specific dominant negative inhibitor of TNF actions
Gil et al., Oncogene 2000 : Apoptosis induction by PKR involves phosphorylation of the translational factor eIF-2alpha and activation of the transcriptional factor NF-kappaB , but caspase pathways activated by PKR are not known
Kiemer et al., J Hepatol 2000 (Ischemia...) : 8-Br-cGMP mimicked the effects of ANP on NF-kappaB and AP-1
Min et al., J Exp Med 2000 (Calcinosis...) : Although OPG is normally expressed in arteries, OPG ligand (OPGL) and receptor activator of NF-kappaB ( RANK ) are not detected in the arterial walls of wild-type adult mice
Rhee et al., J Biol Chem 2000 : However, the truncated form ( delta Tlr4 ( P712H ) ) of the missense mutant Tlr4 ( P712H ) found in LPS-hyporesponsive mouse strain ( C3H/HeJ ) inhibits LPS induced NF kappa B activation and COX-2 expression ... However, the truncated form ( delta Tlr4 ( P712H ) ) of the missense mutant Tlr4 ( P712H ) found in LPS-hyporesponsive mouse strain ( C3H/HeJ ) inhibits LPS induced NF kappa B activation and COX-2 expression ... The inability of delta Tlr4 ( P712H ) to activate NF kappa B and induce COX-2 expression is rescued by a constitutively active adapter protein myeloid differentiation factor 88 ( MyD88 ), which interacts directly with the cytoplasmic domain of Tlr proteins
Jan et al., J Virol 2000 (Neuroblastoma) : The finding that AACOCF ( 3 ) and SOD significantly block activation of NF-kappaB suggests that this activation is derived from the AA-superoxide anion pathway
Daun et al., Am J Physiol Regul Integr Comp Physiol 2000 : Physiological doses of hydrocortisone ( 50-200 ng/ml ) diminished both the LPS stimulated decrease in cytosolic IkappaBalpha levels and the subsequent increase in nuclear NF-kappaB DNA binding
Xie et al., Am J Physiol Lung Cell Mol Physiol 2000 : Gel shift and Western blot analyses confirmed that TNF-alpha activated NF-kappaB and depleted IkappaB in this system and that these effects were prevented by MG-132 and pyrrolidine dithiocarbamate
Kim et al., Liver 2000 : Effects of DDB on the expression of xenobiotic metabolizing enzymes and on nuclear factor-kappaB (NF-kappaB) activation and tumor necrosis factor-alpha (TNF-alpha) production by lipopolysaccharide (LPS) , an endotoxin involved in inflammatory responses, were examined in rats and in RAW264.7 cells to investigate mechanistic aspects ... Effects of DDB on the expression of xenobiotic metabolizing enzymes and on nuclear factor-kappaB (NF-kappaB) activation and tumor necrosis factor-alpha (TNF-alpha) production by lipopolysaccharide (LPS), an endotoxin involved in inflammatory responses, were examined in rats and in RAW264.7 cells to investigate mechanistic aspects ... Pretreatment of rats with DDB prevented LPS induced hepatic I-kappaBalpha degradation and the resultant NF-kappaB activation, and inhibited the LPS induced plasma TNF-alpha protein and hepatic TNF-alpha mRNA expression in a dose dependent manner ... Pretreatment of rats with DDB prevented LPS induced hepatic I-kappaBalpha degradation and the resultant NF-kappaB activation , and inhibited the LPS induced plasma TNF-alpha protein and hepatic TNF-alpha mRNA expression in a dose dependent manner
Yamanaka et al., Hepatology 2000 (Carcinoma, Hepatocellular...) : Despite the expression of TRAIL-R1 and -R2, all 6 HCC cell lines showed resistance to TRAIL induced apoptosis with no relation to nuclear factor kappa B (NF-kappaB) levels induced by TRAIL ... Actinomycin D and camptothecin almost completely suppressed NF-kappaB induction by TRAIL , whereas doxorubicin had little effect
Wu et al., J Biol Chem 2000 (Breast Neoplasms) : In addition, ectopic expression of NFkappaB resulted in increased levels of endogeneous BRCA2 expression ... Thus, NFkappaB and USF regulate BRCA2 expression through the BRCA2 promoter
Patel et al., Oncogene 2000 (Breast Neoplasms) : Furthermore, expression of the anti-apoptotic tumor necrosis factor receptor associated factor 1 ( TRAF1 ) and defender-against cell death ( DAD-1 ) is regulated by NF-kappaB in certain breast cancer cells
West et al., J Trauma 2000 : Lipopolysaccharide (LPS) activation of macrophage ( MO ) cytokine secretion requires activation and translocation of nuclear factor-kappaB (NF-kappaB) ... LPS stimulation activated NF-kappaB in both HeN and HeJ MOs
Ayoub et al., Int J Immunopharmacol 2000 (Translocation, Genetic) : We also demonstrate that histaglobin inhibits the nuclear translocation of NF-kappaB in response to TNF-alpha or lipopolysaccharide (LPS) in bone marrow derived macrophages of Balb/c mice ... We also demonstrate that histaglobin inhibits the nuclear translocation of NF-kappaB in response to TNF-alpha or lipopolysaccharide (LPS) in bone marrow derived macrophages of Balb/c mice
Choi et al., Int J Immunopharmacol 2000 : Effects of ACF on the nuclear factor-kappaB (NF-kappaB) activation and tumor necrosis factor-alpha (TNF-alpha) production by lipopolysaccharide (LPS) , an endotoxin, were examined in rat and RAW264.7 cells ... Gel retardation analysis revealed that LPS ( 1 microg/kg ) activated NF-kappaB in the liver, whereas pretreatment of rats with ACF ( 10 mg/kg ) completely prevented the NF-kappaB activation ... Data showed that ACF inhibited LPS induced NF-kappaB activation through inhibition of I-kappaBalpha degradation and TNF-alpha production in both rat and RAW264.7 cells
Rioux et al., Carcinogenesis 2000 : These data suggest that ROS, generated during pulmonary metabolism of NNK could act as signal transduction messengers and activate NF-kappaB , which will subsequently induce COX-1 activity and increase PGE ( 2 ) synthesis
Harnish et al., Endocrinology 2000 : Ligand bound ERalpha did not inhibit NF-kappaB binding to DNA, but it did decrease the histone acetyltransferase activity required for NF-kappaB transcriptional activity ... Coexpression of the transcription coactivator CREB binding protein (CBP), but not steroid receptor coactivator 1a, reversed the ERalpha mediated inhibition of NF-kappaB activity
Brady et al., Biochem J 2000 : The activation of NF-kappaB by CD40 is a key process in facilitating this transcription by promoting the activation of the Cepsilon promoter ... The overexpression of TRAF-6 or NIK was sufficient to activate NF-kappaB and the Cepsilon promoter, whereas their dominant negative counterparts decreased the ability of CD40 to activate NF-kappaB and the Cepsilon promoter
Deshpande et al., FASEB J 2000 : Expression of Rac1N17 blocked TNF induced activation of nuclear factor-kappa B (NF-kappaB) , increased activity of caspase-3, and markedly augmented endothelial cell susceptibility to TNF induced apoptosis ... Expression of Rac1N17 blocked TNF induced activation of nuclear factor-kappa B (NF-kappaB) , increased activity of caspase-3, and markedly augmented endothelial cell susceptibility to TNF induced apoptosis
Delgado et al., J Immunol 2000 : We showed previously that VIP/PACAP inhibit NF-kappaB nuclear translocation through the stabilization of IkappaB and reduce IFN regulatory factor-1 (IRF-1) binding to the regulatory elements found in the IL-12 p40 and inducible NO synthase promoters ... We showed previously that VIP/PACAP inhibit NF-kappaB nuclear translocation through the stabilization of IkappaB and reduce IFN regulatory factor-1 (IRF-1) binding to the regulatory elements found in the IL-12 p40 and inducible NO synthase promoters
Kim et al., J Immunol 2000 : We evaluated the role of NF-kappaB in HRV-16 induced IL-8 and IL-6 production by EMSA using oligonucleotides corresponding to the binding sites for NF-kappaB in the IL-6 and IL-8 gene promoters ... We evaluated the role of NF-kappaB in HRV-16 induced IL-8 and IL-6 production by EMSA using oligonucleotides corresponding to the binding sites for NF-kappaB in the IL-6 and IL-8 gene promoters ... Sulfasalazine and calpain inhibitor I, inhibitors of NF-kappaB activation, blocked HRV-16 induced formation of NF-kappaB complexes with oligonucleotides from both cytokines, but did not inhibit mRNA induction for either cytokine ... Thus, HRV-16 induces epithelial expression of IL-8 and IL-6 by an NF-kappaB independent pathway, whereas induction of GM-CSF is at least partially dependent upon NF-kappaB activation ... Thus, HRV-16 induces epithelial expression of IL-8 and IL-6 by an NF-kappaB independent pathway, whereas induction of GM-CSF is at least partially dependent upon NF-kappaB activation ... Thus, HRV-16 induces epithelial expression of IL-8 and IL-6 by an NF-kappaB independent pathway, whereas induction of GM-CSF is at least partially dependent upon NF-kappaB activation
Possel et al., Glia 2000 : By contrast, LPS and interferon-gamma led to translocation of NF-kappaB in microglia and in astrocytes, demonstrating that both cell types are responsive to the stimulus ... By contrast, LPS and interferon-gamma led to translocation of NF-kappaB in microglia and in astrocytes, demonstrating that both cell types are responsive to the stimulus
Raychaudhuri et al., Cytokine 2000 : IL-10 also inhibited TNF secretion and NF-kappa B activation induced by another stimulus, staphylococcal toxin
Sharma et al., Bioorg Med Chem 2000 : The metal-interaction of aurine tricarboxylic acid (ATA) and its inhibitory effect on the DNA binding of NFkappaB were studied
Huang et al., Cytokines Cell Mol Ther 2000 (Lung Neoplasms...) : The purpose of this study was to determine whether constitutive NF-kappaB activity regulates the expression level of interleukin-8 (IL-8) in metastatic human melanoma cells ... Transfection of melanoma cells with a dominant negative mutant IkappaBalpha expression vector ( pLXSN-IkappaBalphaM ) significantly decreased the level of constitutive NF-kappaB activity and expression of IL-8, demonstrating that constitutive NF-kappaB/relA activities contribute to overexpression of IL-8 in highly metastatic human melanoma cells
Kurokouchi et al., J Bone Miner Res 2000 : In the present study, we investigated effects of a synthetic glucocorticoid, dexamethasone ( Dex ), on TNF-alpha dependent activation of NF-kappaB and expression of the ICAM-1 gene ... Electrophoretic mobility shift assay ( EMSA ) revealed that TNF-alpha dependent activation of NF-kappaB was almost completely suppressed by Dex treatment
Kim et al., J Korean Med Sci 2000 (Acute Disease...) : Present study aims to investigate whether neutrophils primed by 4beta-phorbol 12beta-myristate 13alpha-acetate ( PMA ) affect the productions of H2O2 and lipid peroxide (LPO), NF-kappaB activation and cytokine production in pancreatic acinar cells, and whether these alterations were inhibited by N-acetylcysteine (NAC) and superoxide dismutase ( SOD ) ... Present study aims to investigate whether neutrophils primed by 4beta-phorbol 12beta-myristate 13alpha-acetate ( PMA ) affect the productions of H2O2 and lipid peroxide (LPO), NF-kappaB activation and cytokine production in pancreatic acinar cells, and whether these alterations were inhibited by N-acetylcysteine (NAC) and superoxide dismutase ( SOD )
Ouchi et al., Circulation 2000 : Adiponectin , an adipocyte derived plasma protein, inhibits endothelial NF-kappaB signaling through a cAMP dependent pathway ... Adiponectin suppressed TNF-alpha induced IkappaB-alpha phosphorylation and subsequent NF-kappaB activation without affecting other TNF-alpha mediated phosphorylation signals, including Jun N-terminal kinase, p38 kinase, and Akt kinase ... Adiponectin suppressed TNF-alpha induced IkappaB-alpha phosphorylation and subsequent NF-kappaB activation without affecting other TNF-alpha mediated phosphorylation signals, including Jun N-terminal kinase, p38 kinase, and Akt kinase
Malka et al., Gastroenterology 2000 : We examined whether TNF-alpha induction of PAP I expression was mediated by NF-kappaB or MAP kinases by using specific inhibitors of both pathways ... We examined whether TNF-alpha induction of PAP I expression was mediated by NF-kappaB or MAP kinases by using specific inhibitors of both pathways
Rauch et al., FEBS Lett 2000 : PDGF induced Akt phosphorylation does not activate NF-kappa B in human vascular smooth muscle cells and fibroblasts ... The present study investigates the role of Akt in the activation of NF-kappa B by tumor necrosis factor-alpha ( TNF alpha, 10 ng/ml ) and PDGF-BB ( 20 ng/ml ) in human vascular smooth muscle cells ( SMC ), skin and foreskin fibroblasts ... The present study investigates the role of Akt in the activation of NF-kappa B by tumor necrosis factor-alpha ( TNF alpha, 10 ng/ml ) and PDGF-BB ( 20 ng/ml ) in human vascular smooth muscle cells ( SMC ), skin and foreskin fibroblasts ... The present study investigates the role of Akt in the activation of NF-kappa B by tumor necrosis factor-alpha ( TNF alpha, 10 ng/ml ) and PDGF-BB ( 20 ng/ml ) in human vascular smooth muscle cells ( SMC ), skin and foreskin fibroblasts ... TNF alpha stimulated serine phosphorylation and degradation of the inhibitory protein I kappa B alpha and strongly induced nuclear NF-kappa B translocation and binding activity ... TNF alpha stimulated serine phosphorylation and degradation of the inhibitory protein I kappa B alpha and strongly induced nuclear NF-kappa B translocation and binding activity ... These data suggest that Akt phosphorylation is not involved in NF-kappa B activation in human SMC and fibroblasts
Mori et al., Cancer Res 2000 (HTLV-I Infections...) : Through the use of an electrophoretic mobility shift assay, we demonstrated that Tax induced NF-kappaB binding to both MCP-1 kappaB sites
Bonnard et al., EMBO J 2000 : Tumor necrosis factor receptor associated factor 2 ( TRAF2 ) plays a role in NF-kappaB activation in response to cytokines such as tumor necrosis factor alpha (TNFalpha) ... However, T2K has a unique role in the activation of NF-kappaB directed transcription, apparently independent of I-kappaB degradation and NF-kappaB DNA binding
Bours et al., Bull Mem Acad R Med Belg 1999 (Adenocarcinoma...) : In these cells, p100 sequesters NF-kappa B in the cytoplasm and blocks the induction of NF-kappa B-dependent genes in response to stimuli such as TNF-alpha
Guo et al., Immunopharmacology 2000 : Antisense IRAK-1 oligonucleotide blocks activation of NF-kappa B and AP-1 induced by IL-18 ... Antisense IRAK-1 oligonucleotide blocks activation of NF-kappa B and AP-1 induced by IL-18 ... Interleukin-1 receptor associated kinase-1 ( IRAK-1 ) has recently been shown to be involved in IL-18 stimulated activation of NF-kappaB and AP-1 ... The purpose of this study is to investigate the effects of preventing IRAK-1 expression by antisense IRAK-1 oligodeoxynucleotide ( ODN ) on IL-18 stimulated activation of NF-kappaB and AP-1 ... As a result, antisense IRAK-1 ODN attenuated IL-18 induced activation of NF-kappaB and AP-1 as measured by sandwich ELISA in a concentration ( 1-8 microg ml(-1) ) - and time ( 5-24 h ) -dependent fashion
Jeon et al., Immunopharmacology 2000 : In the present study, we investigated the role of CD14 and complement receptor type 3 ( CR3 ) in mediating NO production and NF-kappaB/Rel activation induced by angelan and LPS ... In the present study, we investigated the role of CD14 and complement receptor type 3 ( CR3 ) in mediating NO production and NF-kappaB/Rel activation induced by angelan and LPS ... Third, angelan induced strong NF-kappaB/Rel and slight AP-1 DNA binding, whereas LPS potently activated both NF-kappaB/Rel and AP-1
Bourke et al., J Biol Chem 2000 : The present study shows that interleukin-1 (IL-1) causes persistent activation of NF-kappaB in glial cells ... In addition, in vivo overexpression of the various IkappaB isoforms revealed that IkappaB-beta is the only isoform that has the ability to inhibit IL-1 induced NF-kappaB-driven transcription
Brigelius-Flohé et al., Atherosclerosis 2000 : Overexpression of PHGPx inhibits hydroperoxide induced oxidation, NFkappaB activation and apoptosis and affects oxLDL mediated proliferation of rabbit aortic smooth muscle cells
Chaudhary et al., Oncogene 2000 : Dominant negative mutants of the Caspase 8 prodomain can block NF-kappaB induced by Caspase 8 , FADD and several death receptors belonging to the TNFR family ... Dominant negative mutants of the Caspase 8 prodomain can block NF-kappaB induced by Caspase 8, FADD and several death receptors belonging to the TNFR family
Kim et al., Oncogene 2000 : In this study, we demonstrate that the proteolytic cleavage of receptor interacting protein ( RIP ) by caspase-8 during TNF induced apoptosis abrogates the stimulatory role of RIP on TNF induced NF-kappaB activation ... Ectopic expression of the pro-apoptotic C-terminal fragment of RIP inhibited TNF induced NF-kappaB activation by suppressing the activity of I-kappaB kinasebeta (IKKbeta) which phosphorylates I-kB, an inhibitor of NF-kappaB, and triggers its ubiquitin mediated degradation ... Ectopic expression of the pro-apoptotic C-terminal fragment of RIP inhibited TNF induced NF-kappaB activation by suppressing the activity of I-kappaB kinasebeta (IKKbeta) which phosphorylates I-kB, an inhibitor of NF-kappaB, and triggers its ubiquitin mediated degradation
Rahman et al., Am J Physiol Cell Physiol 2000 : We observed that PKC-zeta also induced the TNF-alpha induced NF-kappaB binding to the ICAM-1 promoter and the resultant ICAM-1 gene transcription
Li et al., Am J Physiol Regul Integr Comp Physiol 2000 : We recently reported that tumor necrosis factor-alpha (TNF-alpha) rapidly activates NF-kappaB in differentiated skeletal muscle myotubes and that TNF-alpha acts directly on the muscle cell to induce protein degradation
Zaninovic et al., Am J Physiol Gastrointest Liver Physiol 2000 (Pancreatitis) : Inhibitory analysis showed that activation of transcription factor nuclear factor-kappaB (NF-kappaB) was involved in ICAM-1 upregulation by cerulein, but NF-kappaB did not mediate basal expression of ICAM-1 mRNA in acinar cells
Zhang et al., Cell Immunol 2000 : NF-kappa B regulates the LPS induced expression of interleukin 12 p40 in murine peritoneal macrophages : roles of PKC, PKA, ERK, p38 MAPK, and proteasome ... In this report we describe our investigation of the mechanisms of LPS induced NF-kappa B activation and IL-12 expression in murine peritoneal suppressor macrophages ... The results in this paper indicate that LPS induced NF-kappa B binding activity was independent of PKC, PKA, ERK, and p38 MAPK, but was regulated by proteasome ... The results in this paper indicate that LPS induced NF-kappa B binding activity was independent of PKC, PKA, ERK, and p38 MAPK , but was regulated by proteasome ... The results in this paper indicate that LPS induced NF-kappa B binding activity was independent of PKC, PKA, ERK , and p38 MAPK, but was regulated by proteasome ... The results in this paper indicate that LPS induced NF-kappa B binding activity was independent of PKC, PKA , ERK, and p38 MAPK, but was regulated by proteasome
Iseki et al., Biochem Biophys Res Commun 2000 : Pyrrolidine dithiocarbamate inhibits TNF-alpha dependent activation of NF-kappaB by increasing intracellular copper level in human aortic smooth muscle cells ... In the present study, we investigated effects of PDTC and another antioxidant, N-acetyl-l-cysteine (NAC), on TNF-alpha dependent activation of NF-kappaB in human aortic smooth muscle cells ( HASMC ) ... These results indicate that TNF-alpha dependent expression of MCP-1 in HASMC is tightly regulated by NF-kappaB and that intracellular copper level is crucial for the TNF-alpha dependent activation of NF-kappaB in HASMC ... These results indicate that TNF-alpha dependent expression of MCP-1 in HASMC is tightly regulated by NF-kappaB and that intracellular copper level is crucial for the TNF-alpha dependent activation of NF-kappaB in HASMC
Aggarwal et al., Biochem Pharmacol 2000 : The nuclear factor (NF)-kappa B and the genes regulated by this transcription factor have been shown to play a critical role in induction of resistance to killer agents
Rahman et al., Biochem Pharmacol 2000 (Lung Diseases) : The promoter regions of the human gamma-GCS subunits contain AP-1, NF-kappa B , and antioxidant response elements and are regulated by oxidants, growth factors, inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) , and anti-inflammatory agent ( dexamethasone ) in lung cells ... TNF-alpha also induces the activation of NF-kappa B and AP-1 and the subsequent increase in gamma-GCS heavy subunit transcription in these cells
Bours et al., Biochem Pharmacol 2000 (Neoplasms) : It has also been reported that NF-kappa B activation by tumor necrosis factor (TNF)-alpha , chemotherapeutic drugs, or ionizing radiations can protect several cell types against apoptosis, suggesting that NF-kappa B could participate in resistance to cancer treatment
Vanden Berghe et al., Biochem Pharmacol 2000 : Furthermore, the enhanceosome displays histone acetylation activity, which turned out to be essential for IL-6 gene activation via NF-kappa B ... However, activation of NF-kappa B alone is not sufficient for IL-6 gene induction in response to TNF, as inhibition of the coactivated extracellular signal regulated kinase and p38 MAPK pathways blocks TNF mediated gene expression
Lee et al., Science 2000 (Cachexia...) : Failure to regulate TNF induced NF-kappaB and cell death responses in A20-deficient mice ... A20-deficient cells fail to terminate TNF induced NF-kappaB responses ... Thus, A20 is critical for limiting inflammation by terminating TNF induced NF-kappaB responses in vivo
Guttridge et al., Science 2000 (Cachexia) : The transcription factor nuclear factor kappa B (NF-kappaB) is activated by the cytokine tumor necrosis factor (TNF) , a mediator of skeletal muscle wasting in cachexia ... In differentiating C2C12 myocytes, TNF induced activation of NF-kappaB inhibited SMD by suppressing MyoD mRNA at the posttranscriptional level ... In differentiating C2C12 myocytes, TNF induced activation of NF-kappaB inhibited SMD by suppressing MyoD mRNA at the posttranscriptional level
Foryst-Ludwig et al., J Biol Chem 2000 : Here we report that in H. pylori infection p21 activated kinase 1 (PAK1) activates NF-kappaB
Inan et al., Mol Carcinog 2000 : The p106 and p112 proteins bound to NF-kappaB, and their levels changed during the transient interleukin-1beta activation of NF-kappaB in HT-29 cells
Deng et al., J Biol Chem 2001 : Based on these results, we conclude that 2-AAF up-regulates mdr1b through the generation of ROS, activation of IKK kinase, degradation of I kappa B beta , and subsequent activation of NF-kappa B
Pousset et al., Eur Cytokine Netw 2000 : Using electrophoretic mobility shift assay ( EMSA ), we show that IL-1beta induced NFkappaB activation in primary culture of mouse astrocytes is mediated by the interaction of this cytokine with the IL-1 type I receptor/IL-1 receptor accessory protein complex, as demonstrated by the ability of blocking monoclonal antibodies against these receptors to attenuate NFkappaB activation ... Furthermore, anti-inflammatory cytokines such as IL-4 and IL-10 that block IL-1b induced NFkappaB activation also attenuate IL-1beta induced Akt phosphorylation, despite the fact that IL-4 and IL-10 in isolation induced Akt phosphorylation ... All these findings point to an interaction between Akt and NFkappaB dependent IL-1 signaling in the primary culture of astrocytes
Barchowsky et al., Cytokine 2000 (MAP Kinase Signaling System) : Although both IL-1 and TNF activate NF-kappaB in these cells, only IL-1 induces collagenase-1 transcription ... Although both IL-1 and TNF activate NF-kappaB in these cells, only IL-1 induces collagenase-1 transcription ... We have reported previously that NF-kappaB and AP-1 cooperate to mediate IL-1 induced MMP-1 transcription
Foehr et al., J Neurosci 2000 : Although both p75 and TrkA activated nuclear factor-kappaB (NF-kappaB) , we show that distinct proximal signaling intermediates are used by each receptor ... Although both p75 and TrkA activated nuclear factor-kappaB (NF-kappaB) , we show that distinct proximal signaling intermediates are used by each receptor ... A dominant negative mutant of TRAF6 blocked p75- but not TrkA mediated induction of NF-kappaB ... Conversely a dominant negative mutant of Shc inhibited TrkA but not p75 activation of NF-kappaB ... Blocking p75 mediated activation of NF-kappaB by ecdysone-inducible expression of a nondegradable mutant of IkappaBalpha significantly enhanced apoptosis ... Blocking p75 mediated activation of NF-kappaB by ecdysone-inducible expression of a nondegradable mutant of IkappaBalpha significantly enhanced apoptosis
Henry et al., Mol Cell Biol 2000 : Here we show that expression of activated Ral in quiescent rodent fibroblasts is sufficient to induce activation of NF-kappaB dependent gene expression and cyclin D1 transcription, two key convergence points for mitogenic and survival signaling ... The regulation of cyclin D1 transcription by Ral is dependent on NF-kappaB activation and is mediated through an NF-kappaB binding site in the cyclin D1 promoter ... Ral activation of these responses is likely through an as yet uncharacterized effector pathway, as we find activation of NF-kappaB and the cyclin D1 promoter by Ral is independent of association of Ral with active phospholipase D1 or Ral binding protein 1, two proteins proposed to mediate Ral function in cells ... Ral activation of these responses is likely through an as yet uncharacterized effector pathway, as we find activation of NF-kappaB and the cyclin D1 promoter by Ral is independent of association of Ral with active phospholipase D1 or Ral binding protein 1, two proteins proposed to mediate Ral function in cells
Yi et al., Biochem Biophys Res Commun 2000 : Deletion analyses showed that the response-element resides within the promoter region of -69 bp and that an NF-kappaB binding site at -41 bp is involved in the Tax-activation of the FN promoter ... Overexpression of NF-kappaB enhanced the Tax-activation of the FN promoter
Nolan et al., Shock 2000 : We hypothesize that NF-kappaB translocation is necessary for the induction of TRAF-1 in PMNs with prolonged survival ... These data demonstrate that inhibition of PMN apoptosis and TRAF-1 induction by LPS and TNFalpha is NF-kappaB dependent
Cantin et al., Am J Respir Crit Care Med 2000 : The degree to which albumin increased GSH levels was sufficient to protect cells against H ( 2 ) O ( 2 ) -mediated cytotoxicity and to decrease TNF-alpha mediated NF-kappaB activation ... We conclude that albumin specifically modulates cellular GSH levels, an effect sufficient to protect cells against oxidant injury and regulate NF-kappaB activation
Delgado et al., J Biol Chem 2001 (MAP Kinase Signaling System) : First, VIP/PACAP inhibit p65 nuclear translocation and NF-kappa B DNA binding by stabilizing the inhibitor I kappa B alpha ... First, VIP/PACAP inhibit p65 nuclear translocation and NF-kappa B DNA binding by stabilizing the inhibitor I kappa B alpha ... First, VIP/PACAP inhibit p65 nuclear translocation and NF-kappa B DNA binding by stabilizing the inhibitor I kappa B alpha ... The VIP/PACAP inhibition of NF-kappa B at various levels and through different transduction pathways could offer a significant advantage over other anti-inflammatory agents ... The VIP/PACAP inhibition of NF-kappa B at various levels and through different transduction pathways could offer a significant advantage over other anti-inflammatory agents
Obara et al., Arterioscler Thromb Vasc Biol 2000 (Arteriosclerosis) : Electromobility gel shift and luciferase assays demonstrated that overexpression of IkappaBDeltaN inhibited NF-kappaB activation induced by TNF-alpha or interleukin-1beta (IL-1beta) ... Electromobility gel shift and luciferase assays demonstrated that overexpression of IkappaBDeltaN inhibited NF-kappaB activation induced by TNF-alpha or interleukin-1beta (IL-1beta)
Brennan et al., J Biol Chem 2001 : It inhibits LMP1 stimulated NFkappaB , STAT, and Jun transcriptional activity
Sakai et al., Can J Anaesth 2000 : Because NF-kappaB is likely involved in brain injury and inflammatory neurodegenerative disease, such as multiple sclerosis, we examined whether ketamine inhibits LPS induced activation of NF-kappaB in human glioma cells in vitro and intact mouse brain cells in vivo
Hao et al., J Clin Invest 2000 (Dehydration) : RMIC COX2 expression was also induced by driving NF-kappaB activation with a constitutively active IkappaB kinase alpha (IKKalpha)
Andjelic et al., J Immunol 2000 : Furthermore, our data indicate that PI-3K is indispensable for CD40 mediated NF-kappaB/Rel activation
Li et al., Circulation 2000 : Treatment of cells with the antioxidant alpha-tocopherol ( 10 to 50 micromol/L ) attenuated ox-LDL mediated degradation of IkappaBalpha and activation of NF-kappaB ( P65 ) and inhibited the upregulation of AT1R mRNA and protein ... Treatment of cells with the antioxidant alpha-tocopherol ( 10 to 50 micromol/L ) attenuated ox-LDL mediated degradation of IkappaBalpha and activation of NF-kappaB ( P65 ) and inhibited the upregulation of AT1R mRNA and protein
Greene et al., J Immunol 2000 (Sarcoidosis, Pulmonary) : We examined the effects of IL-18 on AP1 and NF-kappaB in Jurkat T cells in vitro ... Examination of transcription factor activation and IL-2 gene expression in Jurkat T cells revealed that sarcoid but not normal ELF activated AP1 and NF-kappaB , induced IL-2 gene transcription, and up-regulated IL-2 protein production
Rudders et al., J Biol Chem 2001 (Inflammation...) : This induction occurs within hours of stimulation and is mediated by NF-kappaB transactivation of the ESE-1 promoter
Abbott et al., Mol Pharmacol 2000 : These findings suggest that NFAT is among the cofactors involved in NFkappaB dependent IL-6 gene induction by Ca ( 2+ ) -mobilizing receptors in vascular smooth muscle cells
Petro et al., J Biol Chem 2001 : Moreover, activation of NF-kappaB in response to BCR signaling is completely abolished in B cells deficient for PLC-gamma2
Van Putten V et al., J Biol Chem 2001 (MAP Kinase Signaling System) : Inhibition of p38 MAP kinase or NF-kappaB had no effect on cPLA(2) expression
Duffey et al., Br J Cancer 2000 (Head and Neck Neoplasms) : These TNF-alpha resistant HNSCC lines expressed TNF receptor I, and exhibited constitutive and TNF-alpha-inducible activation of NF-kappaB as demonstrated by nuclear localization of NF-kappaB p65 by immunohistochemistry ... We conclude that HNSCC that exhibit constitutive and TNF-alpha-inducible activation of transcription factor NF-kappaB are resistant to TNF-alpha, and that inhibition of NF-kappaB sensitizes HNSCC to TNF-alpha caspase mediated cytotoxicity
Zwacka et al., J Gene Med 2000 (Colorectal Neoplasms) : In order to provide further understanding of determinants of TNF-alpha responses, we studied TNF-alpha induced NF-kappaB activation and variable tumour responses
Manna et al., J Immunol 2000 : We tested this hypothesis by pretreating human Jurkat T cells with IFN-alpha, which blocked TNF induced activation of NF-kappaB and AP-1 in a time- and dose dependent manner as determined by EMSA
Bauer et al., Shock 2000 : Adherence alone did not activate p38MAPK or NF-kappaB, but primed Mphi for an augmented response to LPS in activation of p38 , NF-kappaB and in production of TNF-alpha
Holtmann et al., J Biol Chem 2001 : Recently we found that coordinate activation of NF-kappaB and c-Jun N-terminal protein kinase (JNK) is required for strong IL-8 transcription, whereas the p38 MAP kinase ( MAPK ) pathway stabilizes the IL-8 mRNA ... Expression of the MAP kinase kinase kinase ( MAPKKK ) TAK1 together with its coactivator TAB1 in HeLa cells activated all three pathways and was sufficient to induce IL-8 formation, NF-kappaB + JNK2 mediated transcription from a minimal IL-8 promoter, and p38 MAPK mediated stabilization of a reporter mRNA containing IL-8 derived regulatory mRNA sequences
Bertin et al., J Biol Chem 2000 : CARD9 is a novel caspase recruitment domain containing protein that interacts with BCL10/CLAP and activates NF-kappa B ... BCL10/CLAP is an activator of apoptosis and NF-kappaB signaling pathways and has been implicated in B cell lymphomas of mucosa associated lymphoid tissue ... Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli ... Although its role in apoptosis remains to be determined, BCL10 likely activates NF-kappaB through the IKK complex in response to upstream stimuli ... When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB ... When expressed in cells, CARD9 binds to BCL10 and activates NF-kappaB ... We propose here that CARD9 is an upstream activator of BCL10 and NF-kappaB signaling
Woltman et al., J Am Soc Nephrol 2000 : Stimulation of TEC with CD40L results in activation of NF-kappaB and induction of cytokine production by IL-17 and CD40L is prevented by addition of the NF-kappaB inhibitor pyrrolidine dithiocarbamate ... Stimulation of TEC with CD40L results in activation of NF-kappaB and induction of cytokine production by IL-17 and CD40L is prevented by addition of the NF-kappaB inhibitor pyrrolidine dithiocarbamate
Zhang et al., Free Radic Biol Med 2000 (Gastritis...) : The transcription factor nuclear factor kappaB (NF-kappaB) regulates the expression of inducible nitric oxide synthase (iNOS) ... We hypothesized that induction of iNOS in Helicobacter pylori gastritis may be due to NF-kappaB activation
Weber et al., Gastroenterology 2000 : NF-kappaB/Rel activity induced by tumor necrosis factor alpha, 12-O-tetradecanoylphorbol-13-acetate, or overexpression of NF-kappaB inducing kinase, IKK-alpha , IKK-beta, or constitutively active IKK-alpha and IKK-beta mutants was inhibited dose dependently by sulfasalazine ... NF-kappaB/Rel activity induced by tumor necrosis factor alpha, 12-O-tetradecanoylphorbol-13-acetate, or overexpression of NF-kappaB inducing kinase, IKK-alpha, IKK-beta , or constitutively active IKK-alpha and IKK-beta mutants was inhibited dose dependently by sulfasalazine ... NF-kappaB/Rel activity induced by tumor necrosis factor alpha, 12-O-tetradecanoylphorbol-13-acetate, or overexpression of NF-kappaB inducing kinase, IKK-alpha , IKK-beta, or constitutively active IKK-alpha and IKK-beta mutants was inhibited dose dependently by sulfasalazine
Kirshenbaum et al., Clin Invest Med 2000 : Moreover, the Bcl-2 mediated NFkappaB activation was found to be related to changes in the activity of the NFkappaB regulatory protein IkappaBalpha ( inhibitor of kappa beta )
Lee et al., J Biol Chem 2001 (Leukemia, Experimental) : IL-17E induces activation of NF-kappaB and stimulates production of the proinflammatory chemokine IL-8
Jaffray et al., J Gastrointest Surg 2000 (Acute Disease...) : Elastase, carboxypeptidase A, and lipase induced degradation of I kappa B-beta ( but not I kappa B-alpha ), activation of NF-kappa B , and production of TNF-alpha protein, whereas inhibition of I kappa B with pyrrolidine dithiocarbamate attenuated this response ... Elastase, carboxypeptidase A, and lipase induced degradation of I kappa B-beta ( but not I kappa B-alpha ), activation of NF-kappa B , and production of TNF-alpha protein, whereas inhibition of I kappa B with pyrrolidine dithiocarbamate attenuated this response
Tietze et al., Cancer Gene Ther 2000 (Carcinoma, Hepatocellular...) : Several reports have indicated that NFkappaB may also inhibit the programmed cell death induced by cytokines, ionizing radiation, or cytotoxic drugs in some cancer cell lines
Yan et al., J Biol Chem 2001 (Neoplasm Invasiveness) : KiSS-1 represses 92-kDa type IV collagenase expression by down regulating NF-kappa B binding to the promoter as a consequence of Ikappa Balpha -induced block of p65/p50 nuclear translocation ... However, NF-kappaB binding to the MMP-9 promoter required for expression of this collagenase was reduced by KiSS-1 expression
Gründker et al., J Clin Endocrinol Metab 2000 (Cystadenocarcinoma, Serous...) : We conclude that LHRH induces activation of NFkappaB and thus reduces Doxorubicin induced apoptosis in human ovarian cancer cells
Kuinose et al., Acta Med Okayama 2000 : These results suggest that dual signaling consisting of antigen-driven nuclear factor of activated T cells ( NFAT ) activation and LPS mediated NF-kappaB activation is crucial for IL-18 production in macrophages, and that IL-18 can trigger IFN-gamma production in T-cells by MLR
Newton et al., Biochem Biophys Res Commun 2000 : Ceramide induction of COX-2 and PGE ( 2 ) in pulmonary A549 cells does not involve activation of NF-kappaB
Yamashina et al., Biochem Biophys Res Commun 2000 : In Kupffer cells from mice treated with ethanol 1 h earlier, LPS induced TNFalpha production, and IRAK expression and activity and NFkappaB were decreased 50-60 % of control
Fritz et al., J Biol Chem 2001 : Ras related GTPase Rhob represses NF-kappaB signaling ... However, RhoB inhibited both basal and genotoxic agent stimulated activity of the transcription factor nuclear factor kappaB (NF-kappaB) ... Thus, RhoB attenuated alkylation induced increase in the DNA binding activity of NF-kappaB and abrogated NF-kappaB-driven gene expression ... This indicates that RhoB represses NF-kappaB activation by inhibiting dissociation and subsequent degradation of IkappaBalpha
Wang et al., J Biol Chem 2001 (Cell Transformation, Neoplastic) : We show here that either exogenous addition or continuous expression of MGSA/GROalpha in immortalized melanocytes enhances NF-kappaB activation, as well as mitogen activated protein ( MAP ) kinase kinase kinase ( MEKK) 1, MAP kinase kinase (MEK) 3/6, and p38 MAP kinase activation ... Expression of dominant negative M-Ras ( S27N ), dominant negative MEKK1 ( K432M ), or specific chemical inhibitors for p38 MAP kinase ( SB202190 and SB203580 ) block MGSA/GROalpha induced NF-kappaB transactivation, demonstrating that Ras, MEKK1, and p38 are involved in the signal pathways of MGSA/GROalpha activation of NF-kappaB ... Expression of dominant negative M-Ras ( S27N ), dominant negative MEKK1 ( K432M ), or specific chemical inhibitors for p38 MAP kinase ( SB202190 and SB203580 ) block MGSA/GROalpha induced NF-kappaB transactivation, demonstrating that Ras, MEKK1, and p38 are involved in the signal pathways of MGSA/GROalpha activation of NF-kappaB ... Expression of dominant negative M-Ras ( S27N ), dominant negative MEKK1 ( K432M ), or specific chemical inhibitors for p38 MAP kinase ( SB202190 and SB203580 ) block MGSA/GROalpha induced NF-kappaB transactivation, demonstrating that Ras, MEKK1, and p38 are involved in the signal pathways of MGSA/GROalpha activation of NF-kappaB ... Expression of dominant active Ras or dominant active MEKK1 alone can also stimulate NF-kappaB activation ... The expression of dominant negative MEKK1 inhibits the Ras induced NF-kappaB activation, suggesting that MEKK1 is a downstream target of Ras
Das et al., J Biol Chem 2001 : c-Jun NH2-terminal kinase mediated redox dependent degradation of IkappaB : role of thioredoxin in NF-kappaB activation ... In addition, only the reduced form of thioredoxin activated NF-kappaB , whereas the oxidized form was without any effect ... Overexpression of human thioredoxin also caused activation of NF-kappaB and degradation of IkappaB ... On the contrary, dominant negative redox-inactive mutant thioredoxin expression did not activate NF-kappaB , further confirming the redox dependent activation of NF-kappaB ... We also investigated the mechanism of activation of NF-kappaB by thioredoxin ... In contrast, wild-type MEKK1 or JNK kinase induced NF-kappaB activation alone or in combination with thioredoxin expression plasmid
Pereno et al., Oncogene 2000 (Melanoma) : Since TRAF2 activates the transcription factor NF-kappaB , IL-15 through IL-15Ralpha, could have a role in the control of this pathway ... Thus, IL-15Ralpha controls NF-kappaB activation, however differences in the intracellular trafficking of the IL-15 and/or IL-15Ralpha suggest a different biological role for this complex in MELP versus MELREO cells ... Thus, IL-15Ralpha controls NF-kappaB activation, however differences in the intracellular trafficking of the IL-15 and/or IL-15Ralpha suggest a different biological role for this complex in MELP versus MELREO cells
Chen et al., J Immunol 2000 (Lymphoproliferative Disorders) : We have evaluated the in vivo effects of deletion of IkappaB-alpha , a major inhibitor of NF-kappaB , on lymphocyte development, proliferation, and function
Fiebich et al., J Immunol 2000 (Astrocytoma...) : However, activation of NF-kappaB was not involved in SP-induced expression of IL-6
Caamaño et al., J Immunol 2000 (Chronic Disease...) : Together, these results suggest a role for NF-kappaB ( 2 ) in the regulation of lymphocyte apoptosis and a unique role for this transcription factor in maintenance of T cell responses required for long-term resistance to T. gondii
Bureau et al., J Immunol 2000 (Airway Obstruction...) : These results indicate that sustained NF-kappaB activation in asthmatic bronchi is driven by granulocytes and is mediated by IL-1beta and TNF-alpha ... These results indicate that sustained NF-kappaB activation in asthmatic bronchi is driven by granulocytes and is mediated by IL-1beta and TNF-alpha
Manna et al., J Immunol 2000 : Previously, we have demonstrated that leflunomide inhibits TNF induced NF-kappaB activation by suppressing I-kappaBalpha ( inhibitory subunit of NF-kappaB ) degradation ... Previously, we have demonstrated that leflunomide inhibits TNF induced NF-kappaB activation by suppressing I-kappaBalpha ( inhibitory subunit of NF-kappaB ) degradation ... We in this study show that leflunomide also blocks NF-kappaB reporter gene expression induced by TNFR1 , TNFR associated factor 2, and NF-kappaB inducing kinase (NIK), but not that activated by the p65 subunit of NF-kappaB, suggesting that leflunomide acts downstream of NIK
Niwa et al., Eur J Pharmacol 2000 : Furthermore, TNF-alpha increased NF-kappaB DNA binding, which was abolished by pretreatment with pyrrolidine dithiocarbamate
Poggi et al., Eur J Immunol 2000 (Leukemia, Myelomonocytic, Acute) : Engagement of the leukocyte associated Ig-like receptor-1 induces programmed cell death and prevents NF-kappaB nuclear translocation in human myeloid leukemias
Adib-Conquy et al., Am J Respir Crit Care Med 2000 (Sepsis...) : Subsequent in vitro stimulation of PBMC with lipopolysaccharide (LPS) did not induce further NF-kappaB nuclear translocation : the survivors of sepsis and trauma patients showed low expression of both p65p50 and p50p50, whereas nonsurvivors of sepsis showed a predominance of the inactive homodimer and a low p65p50/p50p50 ratio when compared with control subjects
Zhang et al., Blood 2000 : Exposing primary rat hepatocytes to IL-1beta had no effect on IL-6 mediated STAT3 activation ; instead, IL-1beta activated NF-kappaB associated with 2 IL-6 responsive elements ( STAT3 binding site ) on the rat gamma fibrinogen promoter and blocked STAT3 binding to these regions ... Exposing primary rat hepatocytes to IL-1beta had no effect on IL-6 mediated STAT3 activation ; instead, IL-1beta activated NF-kappaB associated with 2 IL-6 responsive elements ( STAT3 binding site ) on the rat gamma fibrinogen promoter and blocked STAT3 binding to these regions
Nishio et al., Life Sci 2000 : The effect of ox-LDL on the transcriptional factor NF-kappaB activation was investigated by electrophoretic mobility shift assay ( EMSA ) ... Ox-LDL caused NF-kappaB activation in VSMCs ... Treatment by transfection of NF-kappaB decoy oligodeoxynucleotides ( ODN ), but not scramble ones, blunted the activation of NF-kappaB by ox-LDL and caused a significant further induction of ox-LDL induced apoptosis
Kang et al., Toxicology 2000 : A specific inhibitor of protein tyrosine kinase ( PTK ) , genistein, prevented the NF-kappaB activation induced by pervanadate in the presence of silica while inhibitors of protein kinase A or C, such as staurosporine or H7, had no inhibitory effect on NF-kappaB activation
Pan et al., Biochem Pharmacol 2000 : Moreover, electrophoretic mobility shift assay ( EMSA ) experiments indicated that curcumin blocked the LPS induced binding of nuclear factor-kappaB (NFkappaB) , a transcription factor necessary for iNOS induction to its ( 32 ) P-labeled double stranded oligonucleotide probe ... These results suggest that curcumin may exert its anti-inflammatory and anti-carcinogenic properties by suppressing the activation of NFkappaB through inhibition of IKK activity
Origuchi et al., J Lab Clin Med 2000 : We also demonstrated immunohistochemically that NAC inhibited NFkappaB nuclear translocation ... These results suggested that NAC inhibited both COX-2 expression and NFkappaB nuclear translocation in MG63, which in turn indicated that NAC could inhibit the inflammatory process involved in bone resorption by regulating COX-2 expression at the level of transcription
Li et al., AIDS Res Hum Retroviruses 2000 (Cell Transformation, Neoplastic...) : In contrast to the transient activation of the NF-kappaB pathway seen in response to cytokines, Tax results in constitutive nuclear levels of NF-kappaB
Sun et al., AIDS Res Hum Retroviruses 2000 : Tax activates NF-kappaB by stimulating the activity of the I-kappaB kinase (IKK), which in turn leads to phosphorylation and degradation of the NF-kappaB inhibitor I-kappaBalpha ... Expression of exogenous IKKgamma in the mutant cells restored NF-kappaB activation, thus confirming the essential role of this regulatory factor in IKK activation by the cellular and viral stimuli
Pise-Masison et al., AIDS Res Hum Retroviruses 2000 : We further demonstrate the importance of Tax activation of NF-kappaB in p53 inhibition, using p65 knockout ( KO ) mouse embryo fibroblasts ( MEFs )
Meja et al., Br J Pharmacol 2000 : 5. Electrophoretic mobility shift assays demonstrated that LPS increased nuclear factor kappa B (NF-kappa B) : DNA binding ... SN50, an inhibitor of NF-kappa B translocation, abolished LPS induced NF-kappaB : DNA binding and the elaboration of TNFalpha, a cytokine known to be regulated by NF-kappaB in monocytes
Kouba et al., J Biol Chem 2001 : Tumor necrosis factor-alpha induces distinctive NF-kappa B signaling within human dermal fibroblasts ... Further, NF-kappa B activation by TNF-alpha was unaffected by overexpression of a dominant negative mutant NIK in fibroblasts, despite detection of endogenous TRAF2 and NIK by Western analysis ... To explore alternative signaling mechanisms in dermal fibroblasts, we found that the intracellular calcium chelator, 3,4,5-trimethoxybenzoic acid, and the calpain inhibitor , N-acetyl-Leu-Leu-norleucinal, both blocked NF-kappa B activation ; however, the specific proteosome inhibitor, lactacystin, failed to do so
Bours et al., Toxicology 2000 (Inflammation...) : We demonstrated that NF-kappaB activation by IL-1beta follows three distinct cell-specific pathways
Braun et al., Neurosci Lett 2000 : GCs can suppress the activity of the redox-sensitive nuclear factor NF-kappaB , which potentially serves neuroprotective functions ... GCs increased the expression of IkappaBalpha , the physiological inhibitor of NF-kappaB
Klein et al., Shock 2000 (Shock, Septic) : LPS induced NF-kappaB translocation was unaffected
Lehmann et al., Hepatology 2000 (Fatty Liver) : SOD1 blunted this increase and reduced the activation of NF-kappaB
Hammermann et al., Mol Pharmacol 2000 : Because nuclear factor-kappaB (NF-kappaB) is essential for induction of iNOS its role for the regulation of CAT-2B expression and L-arginine transport was investigated ... LPS induced increase in iNOS and CAT-2B mRNA was also suppressed by specific NF-kappaB decoy oligodesoxynucleotides, confirming the essential role of NF-kappaB for iNOS and CAT-2B expression ... LPS induced increase in iNOS and CAT-2B mRNA was also suppressed by specific NF-kappaB decoy oligodesoxynucleotides, confirming the essential role of NF-kappaB for iNOS and CAT-2B expression
Chen et al., Mol Pharmacol 2000 : NF-kappaB DNA-protein binding and ICAM-1 promoter activity were enhanced by IL-1beta and these effects were inhibited by tyrphostin 23, but not by PD 98059 or SB 203580
Inan et al., Am J Physiol Gastrointest Liver Physiol 2000 : TNF-alpha is NF-kappaB regulated , and its upregulation in NF-kappaB1 knockouts may result from an alleviation of p50-p50 repression
Werbajh et al., FEBS Lett 2000 : Here we report a role for the nuclear receptor coactivator RAC3 , in modulating NF-kappa B transactivation
Hansen et al., Placenta 2000 : Thus NF-kappaB does not enhance basal or TNF-alpha-responsive PGHS-2 transcription in amnion derived AV-3 cells
Bounou et al., J Biol Chem 2001 : The increase of NF-kappa B activity was abolished when infection with B7-2 bearing HIV-1 particles was performed in the presence of the fusion protein CTLA-4 Ig suggesting that the interaction between virally embedded B7-2 and CD28 on the target cell is responsible for the observed NF-kappa B induction
Kurokouchi et al., J Biol Chem 2001 : To test the hypothesis that fluid flow alters NF-kappaB activation and expression of cell adhesion molecules in osteoblastic cells, we examined the effect of oscillating fluid flow ( OFF ) on tumor necrosis factor (TNF)-alpha induced NF-kappaB activation in rat osteoblast-like UMR106 cells ... The inhibitory effects of OFF on both TNF-alpha induced NF-kappaB activation and intercellular adhesion molecule-1 mRNA expression were shear stress dependent and also increased with OFF exposure duration, indicating that OFF has potent effects on mechanotransduction pathways ... Thus, OFF inhibits TNF-alpha induced IKK activation, leading to a decrease in phosphorylation and degradation of inhibitory IkappaBalpha, which in turn results in the decrease of TNF-alpha induced NF-kappaB activation and potentially the transcription of target genes
Vig et al., J Biol Chem 2001 : Moreover, dominant negative SIMPL blocks IKKalpha- or IKKbeta induced NF-kappaB activity ... These results lead us to propose a model in which SIMPL functions to regulate NF-kappaB activity by linking IRAK/mPLK to IKKbeta/alpha containing complexes
Le et al., J Interferon Cytokine Res 2000 (Adenocarcinoma...) : Collectively, our data demonstrated that constitutive NF-kappaB and AP-1 activation contributes to the overexpression of IL-8 , which in turn plays an important role in tumor angiogenesis and contributes to the aggressive biology of human pancreatic cancer
Kwon et al., Biochem Biophys Res Commun 2000 (Psoriasis) : To investigate the inducing mechanism of IL-6 by IGF-II, we examined the promoter activity of IL-6 and the DNA binding activity of NFkappaB , a strong regulator of IL-6 ... Interestingly, IL-6 promoter activity and the binding activity of NFkappaB were remarkably increased by IGF-II ... Western blot data that IkappaB was reduced by IGF-II significantly suggest that NFkappaB activation by IGF-II may be mediated through the downregulation of IkappaB ... Therefore, these results suggest a novel role of IGF-II in psoriasis possibly by inducing IL-6 through the activation of NFkappaB mediated by downregulation of IkappaB
Beetz et al., Int J Radiat Biol 2000 : NF-kappaB and AP-1 are responsible for inducibility of the IL-6 promoter by ionizing radiation in HeLa cells ... These results demonstrate that NF-kappaB plays a major role in X-ray-inducible IL-6 expression in HeLa cells
Shen et al., Biochem J 2000 : However, pretreatment with the proteasome inhibitor MG132 under conditions that prevented the IL-1beta dependent activation of the nuclear factor NF-kappaB also blocked the inhibitory effect of IL-1beta on IL-6 activated STAT1 ... Taken together, these findings indicate that, by using a proteasome dependent mechanism, IL-1beta concomitantly induces NF-kappaB activation and dephosphorylates IL-6 activated STAT1 ; the latter might partly account for the inhibition by IL-1beta of the IL-6 dependent induction of type II acute-phase genes ... Taken together, these findings indicate that, by using a proteasome dependent mechanism, IL-1beta concomitantly induces NF-kappaB activation and dephosphorylates IL-6 activated STAT1 ; the latter might partly account for the inhibition by IL-1beta of the IL-6 dependent induction of type II acute-phase genes
Ammit et al., Am J Respir Cell Mol Biol 2000 : Accordingly, we examined the effects of TNF- alpha and cAMP on the regulation of nuclear factor (NF)-kappaB , a transcription factor known to modulate cytokine synthesis in numerous cell types ... Stimulation of HASM cells with TNF-alpha increased NF-kappaB DNA binding activity ... However, increased [ cAMP ] ( i ) in HASM neither activated NF-kappaB nor altered TNF-alpha- induced NF-kappaB DNA binding activity
Brasier et al., Mol Cell Biochem 2000 : Although NF-kappaB is ubiquitously expressed, surprisingly the mechanism for Ang II-inducible NF-kappaB regulation differs between aortic smooth muscle cells ( VSMCs ) and hepatocytes
Valladares et al., Endocrinology 2000 : However, TNFalpha induced NF-kappaB DNA binding activity is independent of p38MAPK and ERK activation ... However, TNFalpha induced NF-kappaB DNA binding activity is independent of p38MAPK and ERK activation ... However, TNFalpha induced NF-kappaB DNA binding activity is independent of p38MAPK and ERK activation ... However, TNFalpha induced NF-kappaB DNA binding activity is independent of p38MAPK and ERK activation
Kim et al., J Biol Chem 2001 : Gel shift analysis revealed that VEGF stimulated NF-kappaB activity
Fu et al., J Lipid Res 2000 : These results suggest that activation of both NF-kappaB and PKC signaling pathways is necessary for oxLDL induced ALBP/aP2 gene expression in THP-1 macrophages and that the upregulation of the fatty acid carrier may be a necessary event in foam cell formation ... These results suggest that activation of both NF-kappaB and PKC signaling pathways is necessary for oxLDL induced ALBP/aP2 gene expression in THP-1 macrophages and that the upregulation of the fatty acid carrier may be a necessary event in foam cell formation
Pahan et al., J Biol Chem 2001 : Induction of nitric-oxide synthase and activation of NF-kappaB by interleukin-12 p40 in microglial cells ... Induction of nitric-oxide synthase and activation of NF-kappaB by interleukin-12 p40 in microglial cells ... Because activation of NF-kappaB is important for the expression of iNOS, we investigated the effect of p40 ( 2 ) on the activation of NF-kappaB ... Induction of the DNA binding as well as the transcriptional activity of NF-kappaB by p40 ( 2 ) and inhibition of p40 ( 2 ) -induced expression of iNOS by SN50, a cell-permeable peptide carrying the nuclear localization sequence of p50 NF-kappaB, but not by SN50M, a nonfunctional peptide mutant, suggests that p40 ( 2 ) induces the expression of iNOS through the activation of NF-kappaB ... Induction of the DNA binding as well as the transcriptional activity of NF-kappaB by p40 ( 2 ) and inhibition of p40 ( 2 ) -induced expression of iNOS by SN50, a cell-permeable peptide carrying the nuclear localization sequence of p50 NF-kappaB, but not by SN50M, a nonfunctional peptide mutant, suggests that p40 ( 2 ) induces the expression of iNOS through the activation of NF-kappaB
Hsu et al., Biochem Biophys Res Commun 2000 (Breast Neoplasms) : TNF-alpha can activate nuclear factor-kappa B (NF-kappa B) ... Activation of NF-kappa B by TNF-alpha mediates many functions of TNF-alpha ... In MCF-7 cells ( an estrogen and TNF-alpha receptor positive cell line ), treatment with 17 beta-estradiol ( E ( 2 ) ) inhibited TNF-alpha induced NF-kappa B DNA binding activity in the gel retardation assays
Kobayashi et al., Biochem Biophys Res Commun 2000 : TNF-alpha dependent activation of NF-kappa B in human osteoblastic HOS-TE85 cells is repressed in vector averaged gravity using clinostat rotation ... Effects of vector averaged gravity on tumor necrosis factor (TNF)-alpha dependent activation of nuclear factor kappa B (NF-kappa B) in human osteoblastic HOS-TE85 cells were investigated by culturing the cells using clinostat rotation ( clinorotation ) ... Electrophoretic mobility shift assays revealed that TNF-alpha dependent activation of NF-kappa B was markedly reduced in the clinorotated cells when compared with the cells in control stationary cultures or after horizontal rotation ( motional controls ) ... Consistent with these findings, the TNF-alpha dependent induction of endogenous NF-kappa B-responsive genes p105, I kappa B-alpha, and IL-8, was significantly attenuate in clinorotated cells
Han et al., J Cell Sci 2001 : TNF-alpha stimulates activation of pro-MMP2 in human skin through NF-(kappa)B mediated induction of MT1-MMP
Iordanov et al., Mol Cell Biol 2001 : It has been proposed that PKR plays an essential role in the activation of NF-kappaB by dsRNA ... Here we present evidence that the dsRNA induced NF-kappaB activity and the expression of beta interferon and inflammatory cytokines do not require either PKR or RNase L ... Here we present evidence that the dsRNA induced NF-kappaB activity and the expression of beta interferon and inflammatory cytokines do not require either PKR or RNase L
Brouillard et al., J Biol Chem 2001 : NF-kappa B mediates up-regulation of CFTR gene expression in Calu-3 cells by interleukin-1beta ... Gel shift analysis showed that IL-1beta activated NF-kappaB in Calu-3 cells, and transfection experiments using p50 and RelA expressing vectors showed that exogenous transfected NF-kappaB subunits increased the concentration of CFTR mRNA ... Gel shift analysis with antibody supershifting also showed that IL-1beta caused the binding of NF-kappaB to a kappaB-like response element at position -1103 to -1093 in the CFTR 5'-flanking region ... Transfection experiments using -2150 to +52 CFTR reporter gene constructs showed that the activity of the CFTR promoter is enhanced by exogenous transfected NF-kappaB and IL-1beta and that this enhancement is due, at least in part, to the -1103 to -1093 kappaB site
Tabata et al., J Biol Chem 2001 (Carcinoma, Non-Small-Cell Lung...) : Inhibiting the degradation of IkappaBalpha by pretreatment with the proteasome inhibitor MG-132 significantly inhibited NF-kappaB activation and apoptosis but not DNA damage induced by SN-38 or VP-16
Baur et al., J Endocrinol 2000 : Electrophoretic mobility shift assays ( EMSAs ) revealed that IL-1 beta and TNF alpha activate the transcription factor NF kappa B in reaggregates of rat anterior pituitaries and in TtT/Gf cells cultured alone or cocultured with GH3 cells ... Electrophoretic mobility shift assays ( EMSAs ) revealed that IL-1 beta and TNF alpha activate the transcription factor NF kappa B in reaggregates of rat anterior pituitaries and in TtT/Gf cells cultured alone or cocultured with GH3 cells
Habib et al., J Biol Chem 2001 : NF-kappaB activation by the tumor necrosis factor receptor (TNFR) involves the formation of a multiprotein complex termed a signalosome ... Although previous studies have shown that the activated EGFR can induce NF-kappaB , the mechanism of this activation remains unknown ... Using a dominant negative NIK mutant, we show that NIK activation is required for EGFR mediated NF-kappaB induction ... We also show that a S32/36 IkappaBalpha mutant blocks EGFR induced NF-kappaB activation ... We also show that a S32/36 IkappaBalpha mutant blocks EGFR induced NF-kappaB activation ... Our studies also suggest that a high level of EGFR expression, a frequent occurrence in human tumors, is optimal for epidermal growth factor induced NF-kappaB activation ... Finally, although protein kinase B/Akt has been implicated in tumor necrosis factor and PDGF induced NF-kappaB activation, our studies do not support a role for this protein in EGFR induced NF-kappaB activation
Takayanagi et al., Nature 2000 (Arthritis...) : IFN-gamma induces rapid degradation of the RANK adapter protein, TRAF6 ( tumour necrosis factor receptor associated factor 6 ), which results in strong inhibition of the RANKL induced activation of the transcription factor NF-kappaB and JNK
Andela et al., Cancer Res 2000 (Adenocarcinoma, Bronchiolo-Alveolar...) : The NFkappaB signal blockade resulted in the down-regulation of prometastatic matrix metalloproteinase 9, a urokinase-like plasminogen activator, and heparanase and reciprocal up-regulation of antimetastatic tissue inhibitors of matrix metalloproteinases 1 and 2 and plasminogen activator inhibitor 2
Garbán et al., J Biol Chem 2001 (Ovarian Neoplasms) : TNF-alpha can also promote nuclear factor kappaB (NF-kappaB) activity and regulate the expression of genes that interfere with apoptosis and thus conferring resistance to several apoptotic stimuli ... These findings show that NO might interfere with TNF-alpha dependent NF-kappaB activation by interacting with O ( 2 ) and reducing the generation of H ( 2 ) O ( 2 ), a potent NF-kappaB activator
Pearson et al., J Biol Chem 2001 (Cell Transformation, Neoplastic) : Consistent with results from our focus-formation assays, constitutively active variants of MEK5 and MEK1 synergize to activate NF-kappaB , and MEK5 and ERK5 are required for activation of NF-kappaB by RafBXB ... Consistent with results from our focus-formation assays, constitutively active variants of MEK5 and MEK1 synergize to activate NF-kappaB, and MEK5 and ERK5 are required for activation of NF-kappaB by RafBXB
Iwami et al., J Immunol 2000 : In a mouse macrophage cell line, the exogenously expressed smTLR4 significantly inhibited LPS mediated TNF-alpha production and NF-kappaB activation
Lawless et al., J Immunol 2000 : Following IL-12 pretreatment to induce IL-18R, wild-type, but not Stat4-deficient, activated T cells demonstrated IL-18 induced NF-kappaB DNA binding activity
Sato et al., J Immunol 2000 : LPS induced activation of both NF-kappaB and c-Jun NH ( 2 ) -terminal kinase was severely impaired in MALP-2 pretreated cells
Bowie et al., J Immunol 2000 : Vitamin C inhibits NF-kappa B activation by TNF via the activation of p38 mitogen activated protein kinase
Georganas et al., J Immunol 2000 (Arthritis, Rheumatoid) : Inhibition of NF-kappaB activation significantly reduced the spontaneous and IL-1beta induced secretion of IL-6 and IL-8 by RA FLS and the IL-1ss induced production of IL-6 and IL-8 by human dermal fibroblasts ... Inhibition of NF-kappaB activation significantly reduced the spontaneous and IL-1beta induced secretion of IL-6 and IL-8 by RA FLS and the IL-1ss induced production of IL-6 and IL-8 by human dermal fibroblasts ... Employing gel shift assays, NF-kappaB, C/EBPbeta , and c-Jun were constitutively activated in RA FLS, but only NF-kappaB and c-Jun activity increased after IL-1beta ... Employing gel shift assays, NF-kappaB, C/EBPbeta, and c-Jun were constitutively activated in RA FLS, but only NF-kappaB and c-Jun activity increased after IL-1beta ... NF-kappaB was essential for IL-6 expression, because fibroblasts in which both NF-kappaB p50/p65 genes were deleted failed to express IL-6 in response to IL-1
Ichiyama et al., Antimicrob Agents Chemother 2001 : We examined whether or not clarithromycin inhibits the activation of NF-kappaB induced by tumor necrosis factor alpha (TNF-alpha) or staphylococcal enterotoxin A (SEA) in human monocytic U-937 cells, a T-cell line ( Jurkat ), a pulmonary epithelial cell line ( A549 ), and peripheral blood mononuclear cells ( PBMC ) ... We examined whether or not clarithromycin inhibits the activation of NF-kappaB induced by tumor necrosis factor alpha (TNF-alpha) or staphylococcal enterotoxin A (SEA) in human monocytic U-937 cells, a T-cell line ( Jurkat ), a pulmonary epithelial cell line ( A549 ), and peripheral blood mononuclear cells ( PBMC ) ... Flow cytometry revealed that clarithromycin suppresses NF-kappaB activation induced by TNF-alpha in U-937 and Jurkat cells in a concentration related manner ... Western blot analysis also demonstrated that clarithromycin inhibits NF-kappaB activation induced by TNF-alpha in U-937, Jurkat, and A549 cells and PBMC and by SEA in PBMC
Abraham et al., J Immunol 2001 (Endotoxemia...) : In contrast, alpha-adrenergic blockade before endotoxemia was associated with decreased activation of MEK1/2, ERK2, and CREB , but increased activation of NF-kappa B ... In contrast, alpha-adrenergic blockade before endotoxemia was associated with decreased activation of MEK1/2, ERK2 , and CREB, but increased activation of NF-kappa B ... In contrast, alpha-adrenergic blockade before endotoxemia was associated with decreased activation of MEK1/2 , ERK2, and CREB, but increased activation of NF-kappa B ... In contrast, alpha-adrenergic blockade before endotoxemia was associated with decreased activation of MEK1/2, ERK2 , and CREB, but increased activation of NF-kappa B
Perera et al., J Immunol 2001 : Mitigated induction of COX-2, IL-12 p35, and IL-12 p40 gene expression by CD11b/CD18-deficient macrophages correlated with a marked inhibition of NF-kappa B nuclear translocation and mitogen activated protein kinase ( MAPK ) activation in response to Taxol and of NF-kappa B nuclear translocation in response to LPS
Schwabe et al., Hepatology 2001 : We analyzed the differential role of I kappa B kinase 1 (IKK1) and I kappa B kinase 2 (IKK2) in tumor necrosis factor alpha (TNF-alpha)- and interleukin-1 beta (IL-1 beta) mediated NF-kappa B activation in primary rat hepatocytes ... We analyzed the differential role of I kappa B kinase 1 (IKK1) and I kappa B kinase 2 (IKK2) in tumor necrosis factor alpha (TNF-alpha)- and interleukin-1 beta (IL-1 beta) mediated NF-kappa B activation in primary rat hepatocytes ... Thus, IKK2 is the main mediator for cytokine induced NF-kappa B activation in primary hepatocytes and protects against TNF-alpha induced apoptosis, whereas IKK1 kinase activity is not required for NF-kappa B activation
Uchimura et al., Hepatology 2001 : PPAR gamma:RXR activation also did not affect nuclear factor kappa B (NF-kappa B) nuclear translocation nor NF-kappa B and activator protein 1 (AP-1) activation in the electrophoretic mobility-shift assay ... Finally, PPAR gamma:RXR activation suppressed the LPS induced promoter activity of the NF-kappa B-luciferase reporter gene in RAW 264.7 cells ... Finally, PPAR gamma:RXR activation suppressed the LPS induced promoter activity of the NF-kappa B-luciferase reporter gene in RAW 264.7 cells
Taylor et al., J Acquir Immune Defic Syndr 2000 (Disease Progression...) : Another potential selenoprotein coding function of HIV-1 is associated with the 3 ' end of the nef gene, which terminates in a conserved UGA ( potential Sec ) codon in the context of a sequence ( Cys-Sec ) identical to the C-terminal redox center of thioredoxin reductase , another cellular regulator of NF-kappaB
Lehmann et al., J Rheumatol 2000 : Induction of NF-kappaB DNA binding by the p65 ( RelA ) /p50 heterodimer was observed in response to incubation of Mono Mac 6 cells with SF ( 20 % in culture medium ) from 5 of 8 subjects with RA, 4 of 5 with OA, and none of 3 with undifferentiated seronegative oligoarthritis
Yoo et al., Am J Physiol Lung Cell Mol Physiol 2001 (Pneumonia) : ASA inhibited TNF-alpha induced activation of NF-kappa B by preventing phosphorylation and subsequent degradation of I kappa B-alpha in a prostanoid independent manner ... ASA inhibited TNF-alpha induced activation of NF-kappa B by preventing phosphorylation and subsequent degradation of I kappa B-alpha in a prostanoid independent manner ... ASA inhibited TNF-alpha induced activation of NF-kappa B by preventing phosphorylation and subsequent degradation of I kappa B-alpha in a prostanoid independent manner ... These observations suggest that the anti-inflammatory effect of ASA in lung epithelial cells may be due to suppression of I kappa B kinase activity, which thereby inhibits subsequent phosphorylation and degradation of I kappa B-alpha , activation of NF-kappa B , and proinflammatory cytokine expression in lung epithelial cells
Goebeler et al., Blood 2001 : Multiple signaling pathways regulate NF-kappaB dependent transcription of the monocyte chemoattractant protein-1 gene in primary endothelial cells ... NiCl ( 2 ) -induced MCP-1 synthesis required activation of NF-kappaB since mutation of NF-kappaB binding sites in the promoter resulted in complete loss of inducible promoter activity
Gebel et al., Toxicological sciences : an official journal of the Society of Toxicology 2001 : The activity of NF-kappaB in Swiss 3T3 cells exposed to aqueous extracts of cigarette smoke is dependent on thioredoxin
Das et al., Nat Immunol 2001 (Asthma...) : A critical role for NF-kappa B in GATA3 expression and TH2 differentiation in allergic airway inflammation ... Inhibition of NF-kappa B activity prevented GATA-3 expression and TH2 cytokine production in developing, but not committed, TH2 cells
Sato et al., J Biol Chem 2001 (Anoxia) : A putative NF-kappaB binding site was found in the 5'-flanking region of the xCT gene, but the enhanced expression of xCT by LPS and oxygen was not mediated by NF-kappaB binding
You et al., J Exp Med 2001 : In contrast, IL-1/TNF stimulated nuclear factor (NF)-kappa B DNA binding activity and inhibitor of kappa B ( I kappa B ) phosphorylation was dramatically decreased in Shp-2 ( -/ ) - cells, while the expression and activity of NF-kappa B-inducing kinase (NIK), Akt, and I kappa B kinase (IKK) were not changed ... In contrast, IL-1/TNF stimulated nuclear factor (NF)-kappa B DNA binding activity and inhibitor of kappa B ( I kappa B ) phosphorylation was dramatically decreased in Shp-2 ( -/ ) - cells, while the expression and activity of NF-kappa B-inducing kinase (NIK), Akt, and I kappa B kinase (IKK) were not changed
Gobin et al., Hum Immunol 2000 : HLA-E is not inducible by NF-kappaB or IRF1, but is responsive to IFN-gamma through an upstream STAT1 binding site ... HLA-F is inducible by NF-kappaB through the kappaB1 site of enhancer A, is responsive to IFN-gamma through the ISRE, and is inducible by CIITA
Delgado et al., J Immunol 2001 (Leukemia L1210...) : VIP/PACAP inhibit the expression and/or DNA binding activity of several transcriptional factors involved in FasL expression, i.e., c-myc, NF-kappaB , NF-ATp, and early growth factors (Egr) 2/3 ... VIP/PACAP inhibit the expression and/or DNA binding activity of several transcriptional factors involved in FasL expression, i.e., c-myc, NF-kappaB , NF-ATp, and early growth factors (Egr) 2/3
Conejo et al., J Cell Physiol 2001 : Furthermore, insulin induced nuclear factor-kappaB (NF-kappaB) DNA binding activity and down-regulated activating protein-1 (AP-1) DNA binding activity throughout the differentiation process ... These inhibitors abolished insulin induction of NF-kappaB DNA binding activity and kappaB-chloramphenicol acetyltransferase (CAT) promoter activity, maintaining expressed cytosolic IkappaB-alpha protein, and increased AP-1 DNA binding activity and TRE-CAT promoter activity
McDonald et al., FASEB J 2001 (Reperfusion Injury...) : Hemorrhage and resuscitation with shed blood resulted in an increase in calpain activity ( heart ), activation of NF-kappaB ( kidney ), expression of iNOS and COX-2 ( kidney ), and the development of multiple organ injury and dysfunction, all of which were attenuated by calpain inhibitor I ( 10 mg/kg i.p. ), administered 30 min prior to hemorrhage ... Hemorrhage and resuscitation with shed blood resulted in an increase in calpain activity ( heart ), activation of NF-kappaB ( kidney ), expression of iNOS and COX-2 ( kidney ), and the development of multiple organ injury and dysfunction, all of which were attenuated by calpain inhibitor I ( 10 mg/kg i.p. ), administered 30 min prior to hemorrhage
Vertegaal et al., Cell Signal 2000 : In agreement with our previous finding that p90 ( rsk1 ) is essential for TPA induced activation of NF-kappaB in Adenovirus 5E1 transformed Baby Rat Kidney cells, we now report that the MEK/ERK/p90 ( rsk1 ) inhibitor U0126 efficiently blocks TPA induced IkappaBalpha processing in these cells
Parmentier et al., Eur Respir J 2000 : The effect of the antioxidant compound, N-acetyl-L-cysteine (NAC), on IL-1beta release and regulation of NF-kappaB in a human myelo-monocytic cell line ( THP-1 ) differentiated into macrophages was studied ... Under the same conditions, NF-kappaB binding was activated by LPS and NAC increased this LPS mediated effect ... Under the same conditions, NF-kappaB binding was activated by LPS and NAC increased this LPS mediated effect
De Caterina et al., Arterioscler Thromb Vasc Biol 2001 : To determine whether nuclear factor (NF)-kappaB is necessary to confer endothelial cell responsiveness to interferon (INF)-gamma in terms of vascular cell adhesion molecule (VCAM)-1 expression and leukocyte adhesion, human endothelial cells were treated with IFN-gamma in the presence of low concentrations ( LCs ) of interleukin (IL)-1alpha ( < /=100 pg/mL ), which activates NF-kappaB but does not induce VCAM-1 expression ... Electrophoretic mobility shift assays and immunoblotting of nuclear extracts showed that IFN-gamma activated signal transducers and activators of transcription ( STAT ) -1alpha and interferon regulatory factor (IRF)-1 but not NF-kappaB, whereas LC IL-1alpha activated NF-kappaB but not STAT-1alpha or IRF-1
Qu et al., FASEB J 2001 : We examined a new mechanism of intestinal iNOS regulation with respect to the role of nNOS and its effect on NF-kappaB ... We conclude that in normal rat small intestine, nNOS suppresses the gene expression of iNOS through NF-kappaB down-regulation and that nNOS suppression leads to IkappaBalpha degradation, NF-kappaB activation, and iNOS expression
Nishibe et al., Blood 2001 (MAP Kinase Signaling System) : Activation of NF-kappaB by OSM did not require IkappaB-alpha degradation ... Activation of NF-kappaB by OSM did not require IkappaB-alpha degradation ... Further, inhibition of Erk-1/2 protein by antisense treatment resulted in suppression of TF mRNA expression, indicating a role for Erk-1/2 in modulating NF-kappaB DNA binding activity ... Further, inhibition of Erk-1/2 protein by antisense treatment resulted in suppression of TF mRNA expression, indicating a role for Erk-1/2 in modulating NF-kappaB DNA binding activity
Ruckdeschel et al., J Immunol 2001 : This effect was strongly enhanced by additional initiation of LPS signaling ( 80-90 % ), indicating a synergism between LPS induced signal transduction and inhibition of NF-kappaB by YopP
Alfano et al., J Immunol 2001 : Of interest, TNF-alpha mediated activation of the cellular transcription factor NF-kappaB was unaffected by PTX-B
Chakravortty et al., J Immunol 2001 : SA inhibited LPS induced NF-kappaB activation by preventing loss of IkappaB-alpha and -beta ... SA inhibited LPS induced NF-kappaB activation by preventing loss of IkappaB-alpha and -beta ... SA inhibited LPS induced NF-kappaB activation by preventing loss of IkappaB-alpha and -beta ... The SA-mediated loss of Raf-1 also abolished LPS induced NF-kappaB activation as well as the Erk1/2 pathway ... The dominant negative mutant of MAP kinase kinase 1 inhibited both NO production and NF-kappaB activation in LPS stimulated RAW cells
Faure et al., J Immunol 2001 (Inflammation...) : Furthermore, LPS and IFN-gamma act synergistically to induce TLR2 expression in EC, and LPS induced TLR2 expression is NF-kappaB dependent ... Furthermore, LPS and IFN-gamma act synergistically to induce TLR2 expression in EC, and LPS induced TLR2 expression is NF-kappaB dependent ... Furthermore, LPS and IFN-gamma act synergistically to induce TLR2 expression in EC, and LPS induced TLR2 expression is NF-kappaB dependent
Equils et al., J Immunol 2001 : Our studies demonstrate that human Toll-like receptor 4 (TLR4) mediates LPS induced NF-kappaB and HIV-LTR activation in HMEC through IL-1 signaling molecules, namely myeloid differentiation protein, IL-1R associated kinase, TNFR associated factor, and NF-kappaB inducing kinase ... Our studies demonstrate that human Toll-like receptor 4 (TLR4) mediates LPS induced NF-kappaB and HIV-LTR activation in HMEC through IL-1 signaling molecules, namely myeloid differentiation protein, IL-1R associated kinase, TNFR associated factor, and NF-kappaB inducing kinase
Mariner et al., J Immunol 2001 (Leukemia-Lymphoma, Adult T-Cell) : Furthermore, using promoter deletion constructs and gel shift analysis, we defined a functional NF-kappaB binding motif in the human IL-15Ralpha promoter, suggesting that Tax activation of IL-15Ralpha is due , in part, to the induction of NF-kappaB ... Furthermore, using promoter deletion constructs and gel shift analysis, we defined a functional NF-kappaB binding motif in the human IL-15Ralpha promoter, suggesting that Tax activation of IL-15Ralpha is due , in part, to the induction of NF-kappaB
Aupperle et al., J Immunol 2001 (Arthritis, Rheumatoid) : NF-kappa B regulation by I kappa B kinase-2 in rheumatoid arthritis synoviocytes ... However, the IKK1 dn mutant did not inhibit cytokine mediated activation of NF-kappaB or any of the functional assays
Chang et al., J Surg Res 2001 (Disease Models, Animal...) : Therapeutic effect of dimethyl sulfoxide on ICAM-1 gene expression and activation of NF-kappaB and AP-1 in septic rats ... We investigated the therapeutic effect of DMSO on intercellular adhesion molecule 1 ( ICAM-1 ) gene expression and activation of nuclear factor-kappaB (NF-kappaB) and activating protein-1 (AP-1) in a rat model of peritonitis sepsis ... The levels of ICAM-1 mRNA expression and activation of NF-kappaB and AP-1 in livers were determined at 3 and 6 h post-CI ... At 3 h post-CI surgery ( early sepsis ), DMSO treatment at 30 and 60 min post-CI surgery significantly inhibited sepsis induced ICAM-1 mRNA expression and activation of NF-kappaB and AP-1 ... DMSO has no effect on ICAM-1 gene expression and activation of NF-kappaB and AP-1 when administered at 90 min post-CI surgery
Basu et al., Biochem Biophys Res Commun 2001 (Breast Neoplasms) : BIM did not inhibit NF-kappa B activation by TNF but caused activation of caspases and enhanced cleavage of PKC delta and -epsilon
Stangl et al., Biochem Biophys Res Commun 2001 : Homocysteine attenuated TNF-alpha stimulated U-937 adhesion to the endothelial monolayer and reduced TNF-alpha induced activation of the transcription factor NF-kappaB , indicating that NF-kappaB inhibition may play a role in inhibiting expression of adhesion molecules in endothelial cells
Vockerodt et al., Virology 2001 (Burkitt Lymphoma...) : Inhibition of LMP1 mediated NF kappa B activation by constitutive repressive I kappa B-alpha only marginally impaired IL-10 expression in BL2 cells, while SB2035080 at 5 microM ( a specific p38/SAPK2 inhibitor ) led to reduced IL-10 expression ... Inhibition of LMP1 mediated NF kappa B activation by constitutive repressive I kappa B-alpha only marginally impaired IL-10 expression in BL2 cells, while SB2035080 at 5 microM ( a specific p38/SAPK2 inhibitor ) led to reduced IL-10 expression
Dumitru et al., Cell 2000 (MAP Kinase Signaling System...) : LPS stimulation of peritoneal macrophages from these mice did not activate MEK1, ERK1, and ERK2 but did activate JNK, p38 MAPK, and NF-kappaB
Saliou et al., Free Radic Biol Med 2001 (Erythema) : However, NF-kappaB-DNA binding activity was not prevented, suggesting that PBE affects the transactivation capacity of NF-kappaB ... Inhibition of NF-kappaB dependent gene expression by PBE possibly contributes to the observed increase in MED
Hirota et al., FEBS Lett 2001 : An endogenous redox molecule, thioredoxin, regulates transactivation of epidermal growth factor receptor and activation of NF-kappaB by lysophosphatidic acid
Chae et al., Bone 2001 (MAP Kinase Signaling System) : Both the NF-kappaB inhibitors in the presence of SB203580 had a more inhibitory effect on IL-6 release ... However, the specific inhibitor of p38 MAPK , SB203580, had no effect on TNF-alpha induced NF-kappaB activation and both NF-kappaB inhibitors failed to reduce the p38 MAPK activation in the TNF-alpha stimulated osteoblasts ... However, the specific inhibitor of p38 MAPK, SB203580, had no effect on TNF-alpha induced NF-kappaB activation and both NF-kappaB inhibitors failed to reduce the p38 MAPK activation in the TNF-alpha stimulated osteoblasts
Wajant et al., Int J Biochem Cell Biol 2001 : TRAF 2 is currently the best characterized TRAF family member, having a key role in mediating TNF-R1 induced activation of NF-kappaB and JNK
Choi et al., Glia 2001 (Brain Injuries) : NF-kappaB activation by N-CAM and cytokines in astrocytes is regulated by multiple protein kinases and redox modulation ... These findings suggest that activation of NF-kappaB by N-CAM and cytokines in astrocytes involves multiple signals that differentially affect NF-kappaB nuclear translocation, DNA binding, and transactivation
Han et al., Am J Physiol Cell Physiol 2001 : CCK independently activates intracellular trypsinogen and NF-kappaB in rat pancreatic acinar cells ... However, CCK stimulated NF-kappaB activation in Chinese hamster ovary-CCK ( A ) cells, which do not express trypsinogen, indicating that trypsin is not necessary for CCK activation of NF-kappaB
Tone et al., Proc Natl Acad Sci U S A 2001 : The interaction between CD40 and CD40 ligand ( CD154 ) activates NF-kappa B , Jun N-terminal kinase, and Janus kinase/signal transducers and activators of transcription pathways and promotes B cell growth, differentiation, and survival as well as IL-12 production in macrophages and dendritic cells ... The interaction between CD40 and CD40 ligand ( CD154 ) activates NF-kappa B , Jun N-terminal kinase, and Janus kinase/signal transducers and activators of transcription pathways and promotes B cell growth, differentiation, and survival as well as IL-12 production in macrophages and dendritic cells
Ruan et al., J Vasc Res 2001 (Shock, Septic) : Furthermore, we could show by EMSA that anisodamine completely abolished LPS induced NF-kappa B DNA binding activity in nuclear extracts from HUVEC treated with LPS together with anisodamine
Kokura et al., J Vasc Res 2001 : However, both A/R- and redox induced NF kappa B activation was blocked by inhibition of PTK
Huang et al., J Cereb Blood Flow Metab 2001 (Reperfusion Injury...) : Immunohistochemistry showed SOD1 overexpression attenuated ischemia induced NF-kappaB p65 immunoreactivity ... The current findings provide the first evidence that SOD1 overexpression attenuates activation of NF-kappaB after transient FCI in mice and that preventing this early activation may block expression of downstream deleterious genes like c-myc, thereby reducing ischemic damage
Cruz et al., Nitric Oxide 2001 : In addition, LPS induces the activation of NF-kappa B , and tyrphostin B42 prevents the degradation of the cytosolic factor I kappa B-alpha and blocks the translocation of the NF-kappa B p65 protein subunit into the nucleus ... These results indicate that the JAK family of protein kinases and the transcription factor NF-kappa B are involved in the induction of iNOS protein expression in FSDC stimulated with LPS
Haudek et al., Am J Physiol Heart Circ Physiol 2001 (Cardiomyopathies...) : Inhibition of myocyte NF-kappaB activation by overexpression of myocyte I-kappaBalpha is sufficient to block cardiac TNF-alpha production and prevent cardiac dysfunction after LPS challenge
Cho et al., Infect Immun 2001 : The induction of MCP-1 and IL-8 transcripts in the endothelial cells by O. tsutsugamushi was not blocked by the inhibitors of NF-kappaB ... The induction of MCP-1 and IL-8 transcripts in the endothelial cells by O. tsutsugamushi was not blocked by the inhibitors of NF-kappaB
Mori et al., Infect Immun 2001 : Induction of monocyte chemoattractant protein 1 by Helicobacter pylori involves NF-kappaB ... H. pylori infection induces the activation of NF-kappaB via the NIK-IKK signaling complex, leading to MCP-1 gene transcription in gastric epithelial cells
Chakravortty et al., Infect Immun 2001 : Z-VAD-FMK inhibited LPS induced NF-kappaB activation ... Similarly, a caspase 3-specific inhibitor, Z-Asp-Glu-Val-Asp-fluoromethylketone, inhibited NO production, NF-kappaB activation, and JNK/SAPK phosphorylation in LPS stimulated RAW cells
Wei et al., Endocrinology 2001 : We report that RANK-L rapidly induces NF-kappa B activation in both authentic osteoclast precursors, namely bone marrow macrophages, and RAW 264.7 cells, a murine macrophage line also capable of RANK-L mediated osteoclastogenesis ... RANK-L activates NF-kappa B in both bone marrow macrophages and RAW 264.7 cells by serine phosphorylation of I kappa B alpha within 5 min, resulting in rapid I kappa B alpha degradation and resynthesis
Asea et al., Cell Stress Chaperones 2000 (Adenocarcinoma...) : The [Ca2+ ] i inhibitor BAPTA dose dependently inhibited HSP70- but not LPS induced NF-kappaB activity and subsequent augmentation of proinflammatory cytokine TNF-alpha, IL-1beta, and IL-6 production
Saldeen et al., Autoimmunity 2000 : The acid sphingomyelinase inhibitor SR33557 counteracts TNF-alpha mediated potentiation of IL-1beta induced NF-kappaB activation in the insulin producing cell line Rinm5F ... The aim of this study was to determine whether sphingomyelinase ( SMase ) participates in cytokine signaling leading to NF-kappaB activation, iNOS induction and cell death in insulin producing cells ... TNF-alpha activated NF-kappaB in gel shift experiments without inducing iNOS -- as assessed by nitrite formation -- whereas IL-1beta stimulated both NF-kappaB activation and iNOS induction ... TNF-alpha activated NF-kappaB in gel shift experiments without inducing iNOS -- as assessed by nitrite formation -- whereas IL-1beta stimulated both NF-kappaB activation and iNOS induction ... TNF-alpha activated NF-kappaB in gel shift experiments without inducing iNOS -- as assessed by nitrite formation -- whereas IL-1beta stimulated both NF-kappaB activation and iNOS induction
Kefer et al., Shock 2001 : We addressed the role of the redox state of endothelial cells in modulating TNFalpha induced NF-kappaB activity ... The effects of TNFalpha on DNA binding activity of NF-kappaB and expression of mRNA encoding ICAM-1 ( an NF-kappaB activated gene ) were studied in human pulmonary artery endothelial ( HPAE ) cells under basal conditions and after decreasing the intracellular glutathione ( GSH ) concentration ... Stimulation of control cells with TNFalpha resulted in reactive oxygen species ( ROS ) generation and activation of NF-kappaB binding to the ICAM-1 promoter and ICAM-1 transcription ... Thus the oxidative modification of NF-kappaB secondary to the loss of ROS buffering capacity may regulate NF-kappaB binding to ICAM-1 promoter, and thereby ICAM-1 transcription in endothelial cells
Umezawa et al., Anticancer Drug Des 2000 : The designed compound, DHM2EQ, inhibited TNF-alpha induced activation of NF-kappaB and showed a therapeutic effect in mouse rheumatoid arthritis model
Takii et al., Yakugaku Zasshi 2001 : This up-regulation leads to the increasing susceptibility of IL-1RI to IL-1, as the DNA binding of IL-1 induced NF-kappa B and the production of IL-1 induced IL-6 from the fibroblasts are augmented by pretreatment with IFNs
Kang et al., Mol Cell Biochem 2000 : These inhibitory effects of L-NIL and L-NAME on silica- or LPS induced NF-kappaB activation were also observed in primary rat alveolar macrophages ... Specific inhibitors of protein tyrosine kinase , such as genistein and AG494, prevented NF-kappaB activation in SNP- or SIN-1 treated cells, suggesting involvement of tyrosine kinase in the NO signaling pathway leading to NF-kappaB activation ... Metalloporphyrins, such as tetrakis ( N-methyl-4'-pyridyl ) porphyrinato iron ( III ) ( Fe-TMPyP ) and Zn-TMPyP which are known to alter NO-dependent activity, markedly inhibited silica- and LPS induced NF-kappaB activation
Pawlowski et al., Anticancer Res 2000 (Carcinoma, Renal Cell...) : NF-kappa B , although stimulated in these cell lines, does not play a role in the sensitivity of RCC to TRAIL and could play other functions in TRAIL signaling
Cherla et al., J Immunol 2001 : In this study, we have explored the roles of the second messenger NO and the transcription factor NF-kappa B in SDF1 alpha induced T cell migration ... Furthermore, we observed that inhibitors of the transcription factor NF-kappa B , which is linked to NO signaling pathways, also significantly blocked the SDF1 alpha induced chemotactic response
Schindler et al., J Immunol 2001 : NF-kappa B , which mediates IL-18 effects in T cells, was only weakly activated by IL-12 and/or IL-18 in macrophages ... NF-kappa B , which mediates IL-18 effects in T cells, was only weakly activated by IL-12 and/or IL-18 in macrophages
Baumgartner et al., Cell Microbiol 2000 : PI3-K induction of GM-CSF appears to be at the transcriptional level and, consistently, we demonstrate that PI3-K is also involved in the constitutive induction of AP-1 and NF-kappaB , which characterizes Theileria infected leucocytes
Mandal et al., Gastroenterology 2001 (Colorectal Neoplasms) : BA-induced enhancement of DNA fragmentation and stimulation of Bax promoter activity were blocked by the expression of dominant negative mutants of JNK1 or AP1 but not NF-kappaB
Madrigal et al., J Neurochem 2001 : Since glutamate release and subsequent NMDA ( N-methyl-D-aspartate ) receptor activation has been recognized as an early change after exposure to stressful stimuli, and glutamate has been shown to induce iNOS in brain via a NF-kappaB dependent mechanism, we studied the possible role of excitatory amino acids in the induction of iNOS in our model
Wang et al., J Mol Neurosci 2000 (MAP Kinase Signaling System) : Phosphorylation of the common neurotrophin receptor p75 by p38beta2 kinase affects NF-kappaB and AP-1 activities
Manos et al., Cancer Res 2001 : Follow-up analyses demonstrated that, although TNF-alpha activated NF-kappaB through IkappaB-alpha degradation, alpha-Fas treatment led to NF-kappaB activation through a mechanism distinct from IkappaB-alpha degradation
Shinohara et al., Cancer Res 2001 (Lung Neoplasms) : These observations indicate that NF-kappaB activation may play a role in the regulation of KAI1/CD82 expression in lung cancer cells independently of wild-type p53, and suggest that KAI1/CD82 expression may be regulated by interaction with the host microenvironment
Okado-Matsumoto et al., Antioxid Redox Signal 2000 : Although the binding of NF-kappa B to responsive elements is essential for the induction of the NOS II gene, cAMP elevating agents had no effect on NF-kappa B binding to the element, thus eliminating the involvement of NF-kappa B in the suppression of the NOS II gene by high concentrations of cAMP
Aosasa et al., Shock 2001 : The present study shows that the inhibitory effect of GM on the TNFalpha production of activated human monocytes is mediated by the suppression of NFkappaB activation, while the mechanism of UTI inhibiting TNFalpha production of human monocytes may be due to the inhibition of either the translation or secretion of TNFalpha
González-Fernández et al., Atherosclerosis 2001 : TNF-alpha stimulated the translocation of nuclear factor-kappaB (NF-kappaB) , an effect that was not modified by cerivastatin
Jain et al., Nat Immunol 2001 (Ectodermal Dysplasia...) : Overall, the phenotype observed in XHM-ED patients shows that the putative zinc-finger domain of NEMO has a regulatory function and demonstrates the definite requirement of CD40 mediated NF-kappaB activation for B cell immunoglobulin class switching
Engelhardt et al., Antioxid Redox Signal 1999 (Lung Diseases...) : Two such examples have utilized dominant inhibitory forms of the retinoblastoma gene product ( Rb ) and IkappaBalpha which prevent activation of cyclin dependent protein kinases and NF-kappaB , respectively
Viita et al., Antioxid Redox Signal 1999 (Arteriosclerosis) : High expression of human 15-lipoxygenase induces NF-kappaB mediated expression of vascular cell adhesion molecule 1 , intercellular adhesion molecule 1, and T-cell adhesion on human endothelial cells ... High expression of human 15-lipoxygenase induces NF-kappaB mediated expression of vascular cell adhesion molecule 1, intercellular adhesion molecule 1 , and T-cell adhesion on human endothelial cells ... High expression of human 15-lipoxygenase induces NF-kappaB mediated expression of vascular cell adhesion molecule 1 , intercellular adhesion molecule 1, and T-cell adhesion on human endothelial cells ... These cells were used to study the effects of 15-LO on the expression of vascular cell adhesion molecule 1 ( VCAM-1 ) and intercellular adhesion molecule 1 ( ICAM-1 ), activation of nuclear factor kappa B (NF-kappaB) , and T-cell adhesion on endothelial cells ... These cells were used to study the effects of 15-LO on the expression of vascular cell adhesion molecule 1 ( VCAM-1 ) and intercellular adhesion molecule 1 ( ICAM-1 ), activation of nuclear factor kappa B (NF-kappaB) , and T-cell adhesion on endothelial cells ... NF-kappaB activation was greatly potentiated by increased 15-LO activity in the stably transduced cells, and both VCAM-1 and ICAM-1 were significantly induced in these cells in response to tumor necrosis factor-alpha (TNF-alpha) and phorbol 12-myristate 13-acetate ( PMA ) stimulation, as studied by flow cytometry ... NF-kappaB activation was greatly potentiated by increased 15-LO activity in the stably transduced cells, and both VCAM-1 and ICAM-1 were significantly induced in these cells in response to tumor necrosis factor-alpha (TNF-alpha) and phorbol 12-myristate 13-acetate ( PMA ) stimulation, as studied by flow cytometry
Li et al., Antioxid Redox Signal 1999 : In the present study, we analyzed the signaling pathway by which TNF-alpha activates NF-kappaB in myotubes differentiated from C2C12 and rat primary myoblasts ... We found that activation of NF-kappaB by TNF-alpha was blocked by rotenone or amytal, inhibitors of complex I of the mitochondrial respiratory chain
Little et al., Proc Natl Acad Sci U S A 2001 : A short segment within the cytoplasmic domain of the neural cell adhesion molecule ( N-CAM ) is essential for N-CAM induced NF-kappa B activity in astrocytes ... Transfection of astrocytes with a construct encompassing the transmembrane region and the cytoplasmic domain of N-CAM ( designated Tm-Cyto, amino acids 685-839 in the full-length molecule ) inhibited this activation up to 40 %, and inhibited N-CAM induced translocation of NF-kappaB to the nucleus ... N-CAM also activated NF-kappaB in astrocytes from N-CAM knockout mice, presumably through binding to a heterophile ... Overall, these findings define a short segment of the N-CAM cytoplasmic domain that is critical for N-CAM induced activation of NF-kappaB and may be important in other N-CAM mediated signaling
Bendixen et al., Proc Natl Acad Sci U S A 2001 : We show here that IL-4 inhibits receptor activator of NF-kappaB ligand induced osteoclast differentiation through an action on osteoclast precursors that is independent of stromal cells
Shrivastava et al., Antioxid Redox Signal 1999 : TNF induced NF-kappa B activation was inhibited by both superoxide and lipid peroxide quenchers but potentiated by an hydroxyl radical quencher ... Overall, these results suggest that hydroxyl radicals mediate TNF induced apoptosis but not activation of NF-kappa B , AP-1, and JNK ; superoxide radicals mediate NF-kappa B and JNK activation but potentiate apoptosis ; and lipid peroxides are required for all the signals induced by TNF
Sasaki et al., Antioxid Redox Signal 1999 (Myocardial Ischemia...) : Ischemic adaptation attenuates apoptotic cell death, further increases NF-kappaB binding activity and Bcl-2 gene induction , but reduces AP-1 binding activity
Livolsi et al., Eur J Biochem 2001 : Although serine phosphorylation has been shown to induce ubiquitination and subsequent proteasome mediated degradation of I kappa B-alpha, little is known about the mechanisms that lead to release of active NF-kappa B in T cells as a consequence of tyrosine phosphorylation of I kappa B-alpha [ Imbert, V., Rupec, R.A., Livolsi, A., Pahl, H.L., Traenckner, B.M., Mueller-Dieckmann, C., Farahifar, D., Rossi, B., Auberger, P., Baeuerle, P. & Peyron, J.F. ( 1996 ) Cell 86, 787 -- 798 ]
Gil et al., Apoptosis 2000 : The mechanism of apoptosis induction by PKR involves phosphorylation of eIF-2 alpha and activation of NF-kappa B
Takeuchi et al., Antioxid Redox Signal 2000 : Thioredoxin inhibits tumor necrosis factor- or interleukin-1 induced NF-kappaB activation at a level upstream of NF-kappaB inducing kinase ... Thioredoxin inhibits tumor necrosis factor- or interleukin-1 induced NF-kappaB activation at a level upstream of NF-kappaB inducing kinase ... To elucidate the roles of TRX in the signal transduction of the cytokines, we investigated the effects of TRX on NF-kappaB activation induced by cytokine treatment or by overexpression of the signaling molecules ... Our data show that TRX treatment inhibits NF-kappaB dependent transcription at the level of downstream of TRAFs and upstream of NIK : TRX inhibited TRAF2-, TRAF5-, and TRAF6 induced NF-kappaB activation but does not inhibit NIK-, IKKalpha- , and MEKK induced activation ... Our data show that TRX treatment inhibits NF-kappaB dependent transcription at the level of downstream of TRAFs and upstream of NIK : TRX inhibited TRAF2-, TRAF5-, and TRAF6 induced NF-kappaB activation but does not inhibit NIK-, IKKalpha-, and MEKK induced activation ... In addition, we show that TRX inhibits NF-kappaB activation in a manner different from that for SAPK ( stress activated protein kinase ) inhibition
Bancroft et al., Clin Cancer Res 2001 (Carcinoma, Squamous Cell...) : Interruption of IKK mediated activation of NF-kappaB by expression of an inhibitor kappaB alpha mutant ( IkappaB alphaM ) in UM-SCC-9 cells resulted in partial inhibition of expression of IL-8 but not VEGF ... Expression of IL-8 and VEGF in the cell lines was associated with coactivation of both NF-kappaB and AP-1, and U0126 inhibited both NF-kappaB and AP-1 reporter activity in UM-SCC-9 and UM-SCC-11B cells ... Expression of IL-8 and VEGF in the cell lines was associated with coactivation of both NF-kappaB and AP-1, and U0126 inhibited both NF-kappaB and AP-1 reporter activity in UM-SCC-9 and UM-SCC-11B cells
Harimaya et al., Clin Exp Metastasis 2000 (Neoplasm Invasiveness...) : Moreover, antioxidants inhibited TNFalpha induced osteosarcoma cell invasion, motility and NFkappaB nuclear translocation, but not adhesion to laminin or MMP9 expression
Kikumori et al., Biochem J 2001 : We have recently demonstrated that nuclear factor kappaB (NF-kappaB) mediates the tumour necrosis factor alpha (TNF-alpha) dependent expression of the gene encoding interleukin 6 (IL-6) in rat thyroid FRTL-5 cells cultured in the presence of thyrotropin ( TSH ) ... In the present study we investigated how TSH is involved in the activation of NF-kappaB by TNF-alpha in the cells
Cianferoni et al., Blood 2001 : The inhibitory effect of ASA on IL-4 transcription was not mediated by decreased nuclear expression of the known salicylate target nuclear factor (NF)-kappaB and was accompanied by reduced binding of an inducible factor to an IL-4 promoter region upstream of, but not overlapping, the NF of activated T cells- and NF-kappaB binding P1 element ... The inhibitory effect of ASA on IL-4 transcription was not mediated by decreased nuclear expression of the known salicylate target nuclear factor (NF)-kappaB and was accompanied by reduced binding of an inducible factor to an IL-4 promoter region upstream of, but not overlapping, the NF of activated T cells- and NF-kappaB binding P1 element
Matsushima et al., J Exp Med 2001 : Essential role of nuclear factor (NF)-kappaB inducing kinase and inhibitor of kappaB (IkappaB) kinase alpha in NF-kappaB activation through lymphotoxin beta receptor, but not through tumor necrosis factor receptor I ... Essential role of nuclear factor (NF)-kappaB inducing kinase and inhibitor of kappaB (IkappaB) kinase alpha in NF-kappaB activation through lymphotoxin beta receptor, but not through tumor necrosis factor receptor I ... Essential role of nuclear factor (NF)-kappaB inducing kinase and inhibitor of kappaB (IkappaB) kinase alpha in NF-kappaB activation through lymphotoxin beta receptor , but not through tumor necrosis factor receptor I ... Both nuclear factor (NF)-kappaB inducing kinase ( NIK ) and inhibitor of kappaB (IkappaB) kinase ( IKK ) have been implicated as essential components for NF-kappaB activation in response to many external stimuli ... These results demonstrate that both NIK and IKKalpha are essential for the induction of NF-kappaB through LTbetaR, whereas the NIK-IKKalpha pathway is dispensable in TNFR-I signaling
Means et al., J Immunol 2001 (Tuberculosis) : We now demonstrate that expression of a dominant negative TLR2 or TLR4 proteins in RAW 264.7 macrophages partially blocked Mtb induced NF-kappa B activation ... We now demonstrate that expression of a dominant negative TLR2 or TLR4 proteins in RAW 264.7 macrophages partially blocked Mtb induced NF-kappa B activation
Tsuyuki et al., J Immunol 2001 : It is known that NF-kappa B activation is required for transcription of E-selectin , and the current data show that the suppression of E-selectin expression by S-nitroso-N-acetyl-penicillamine pretreatment and thiol deprivation was associated with reduced NF-kappa B DNA binding activity in PAEC
Ide et al., J Nutr 2001 : Effects of SAC on hydrogen peroxide ( H ( 2 ) O ( 2 ) ) or tumor necrosis factor (TNF)-alpha induced nuclear factor (NF)-kappa B activation were determined ... SAC also inhibited H ( 2 ) O ( 2 ) - or TNF-alpha induced NF-kappa B activation
Pennington et al., Mol Cell Biol 2001 : IkappaB kinase dependent chronic activation of NF-kappaB is necessary for p21 ( WAF1/Cip1 ) inhibition of differentiation induced apoptosis of monocytes ... IkappaB kinase dependent chronic activation of NF-kappaB is necessary for p21 ( WAF1/Cip1 ) inhibition of differentiation induced apoptosis of monocytes
Barton et al., Cell 2001 : Ligation of JAM is required for reovirus induced activation of NF-kappaB and apoptosis
Mandrika et al., Biochem Pharmacol 2001 : Effects of melanocortin peptides on lipopolysaccharide/interferon-gamma induced NF-kappaB DNA binding and nitric oxide production in macrophage-like RAW 264.7 cells : evidence for dual mechanisms of action ... We investigated the effects of alpha-MSH, alpha-MSH ( 1-10 ), and alpha-MSH ( 11-13 ) on NO production and nuclear factor-kappaB (NF-kappaB) translocation in RAW 264.7 macrophages
Du et al., J Neuroimmunol 2001 (Encephalomyelitis, Autoimmune, Experimental) : Our data also show that tyrphostin A1 blocks CD40L induced translocation of NF-kappaB to the nucleus, and reduces the activation of IL-12 p40 gene
Yamamoto et al., FEBS Lett 2001 : These results imply that PLC-gamma1 may play a critical role in a hematopoietic cell-specific adapter protein mediated NF-AT and NF-kappaB activation in a non-lymphoid cell
Döffinger et al., Nat Genet 2001 (Ectodermal Dysplasia...) : We also show that the ectodysplasin receptor, DL, triggers NF-kappaB through the NEMO protein, indicating that EDA results from impaired NF-kappaB signaling
Chen et al., Gene Expr 2000 (Wilms Tumor) : Because the human WT1 promoter contains a conserved NF-kappaB site, we investigated whether NF-kappaB also regulates the expression of the human WT1 gene ... We activated NF-kappaB through cytokine stimulation or inhibited NF-kappaB through expression of a NF-kappaB `` super repressor '' in WT1 expressing Wilms tumor, renal carcinoma, and erythroleukemia cultures and examined the level of endogenous WT1 gene expression ... We conclude that despite the presence of conserved NF-kappaB elements in the murine and human WT1 promoters, NF-kappaB is not required to regulate the expression of the WT1 gene in its natural context
Hong et al., Biochem Biophys Res Commun 2001 (Arteriosclerosis...) : Transient transfection study of NF-kappaB promoter construct and electrophoretic mobility shift assay suggested that hematein inhibited both NF-kappaB dependent gene expression and NF-kappaB activation induced by TNF-alpha ... Our results suggest that the down-regulation of VCAM-1 expression by hematein may in part be due to the inhibition of NF-kappaB dependent gene expression
Cho et al., Glia 2001 : We found that neither LPS induced NF-kappaB translocation nor DNA binding activity was affected by cotreatment with NAMDA in BV-2 microglia
Klasen et al., Br J Pharmacol 2001 : 5. Two different inhibitors of the NF-kappa B-pathway also prevented LPS induced increase in arginase activity
Yin et al., Science 2001 : Defective lymphotoxin-beta receptor induced NF-kappaB transcriptional activity in NIK-deficient mice
Purcell et al., Am J Physiol Gastrointest Liver Physiol 2001 : It is not known whether IKK also mediates activation of NF-kappaB by pX ... Here we report that IKK was not essential for activation of NF-kappaB by pX
Held et al., Am J Respir Crit Care Med 2001 : We therefore compared the effects of ventilation ( -10 cm H2O or -25 cm H2O end-inspiratory pressure ) and LPS ( 50 microg/ml ) on nuclear factor (NF)-kappaB activation, chemokine release, and cytokine release in isolated perfused lungs obtained from BALB/C mice ... We found that both LPS and ventilation with -25 cm H2O ( overventilation ; OV ) caused translocation of NF-kappaB , which was abolished by pretreatment with the steroid dexamethasone
Chan et al., Infect Immun 2001 : Induction of inducible nitric oxide synthase-NO* by lipoarabinomannan of Mycobacterium tuberculosis is mediated by MEK1-ERK, MKK7-JNK, and NF-kappaB signaling pathways ... The objective of this investigation was to examine the role of mitogen activated protein ( MAP ) kinase ( MAPK ) and nuclear factor kappaB (NF-kappaB) signaling pathways in the regulation of iNOS and NO* by a mycobacterial cell wall lipoglycan known as mannose capped lipoarabinomannan ( ManLAM ) ... Overexpression of null mutant IkappaBalpha , a potent inhibitor of NF-kappaB activation, confirmed that the IkappaBalpha kinase (IKK)-NF-kappaB signaling pathway enhanced IFN-gamma-ManLAM induced iNOS promoter activity
Wang et al., Infect Immun 2001 : Dominant negative myeloid differentiation protein ( MyD88 ), IL-1 receptor associated kinase (IRAK), NFkappaB inducing kinase (NIK), and IkappaB kinase (IKK) mutant forms completely inhibited micrococcus- and PGN induced activation of NF-kappaB and expression of the gene for IL-8 ... Dominant negative myeloid differentiation protein ( MyD88 ), IL-1 receptor associated kinase (IRAK), NFkappaB inducing kinase (NIK), and IkappaB kinase (IKK) mutant forms completely inhibited micrococcus- and PGN induced activation of NF-kappaB and expression of the gene for IL-8 ... Induction of NF-kappaB was partially inhibited by dominant negative tumor necrosis factor receptor associated kinase 6 ( TRAF6 ) but not TRAF2, whereas induction of IL-8 gene was partially inhibited by both TRAF6 and TRAF2 ... These data indicate that micrococci and PGN induce TLR2 dependent activation of the gene for IL-8 and that this activation requires MyD88, IRAK, NIK, IKK, and NF-kappaB and may also utilize TRAF6 and, to a lesser extent, TRAF2 ... These data indicate that micrococci and PGN induce TLR2 dependent activation of the gene for IL-8 and that this activation requires MyD88, IRAK, NIK, IKK, and NF-kappaB and may also utilize TRAF6 and, to a lesser extent, TRAF2
Dragneva et al., Infect Immun 2001 : Subcytocidal attack by staphylococcal alpha-toxin activates NF-kappaB and induces interleukin-8 production
Stassen et al., J Immunol 2001 : IL-9 and IL-13 production by activated mast cells is strongly enhanced in the presence of lipopolysaccharide : NF-kappa B is decisively involved in the expression of IL-9 ... In the presence of an inhibitor of NF-kappa B activation, the production of IL-9 is strongly decreased, whereas the expression of IL-13 is hardly reduced, and that of IL-4 is not affected at all
Musikacharoen et al., J Immunol 2001 : NF-kappa B and STAT5 play important roles in the regulation of mouse Toll-like receptor 2 gene expression
Haddad et al., Biochem J 2001 : Pretreatment with the non-steroidal anti-inflammatory drug ( NSAID ) indomethacin, an inhibitor of cyclo-oxygenase, blocked the LPS induced activation of NF-kappaB ... These results indicate the involvement of prostanoid dependent ( NSAID-sensitive ) and IL-1 dependent ( IL-1ra-sensitive ) mechanisms mediating LPS induced NF-kappaB translocation and activation, a pathway that is regulated, in part, by a negative feedback mechanism transduced through IkappaB-alpha , the major cytosolic inhibitor of NF-kappaB ... These results indicate the involvement of prostanoid dependent ( NSAID-sensitive ) and IL-1 dependent ( IL-1ra-sensitive ) mechanisms mediating LPS induced NF-kappaB translocation and activation, a pathway that is regulated, in part, by a negative feedback mechanism transduced through IkappaB-alpha, the major cytosolic inhibitor of NF-kappaB
Tsukagoshi et al., Regul Pept 2001 : ANP inhibited the IFN-gamma induced p38 MAP kinase activation, and ANP and SB203580 inhibited NF-kappaB activation ... ANP inhibited the IFN-gamma induced p38 MAP kinase activation, and ANP and SB203580 inhibited NF-kappaB activation ... ANP inhibited the IFN-gamma induced p38 MAP kinase activation, and ANP and SB203580 inhibited NF-kappaB activation
Mizuno et al., Cell Signal 2001 : Here, we examined the effect of EXTL3 on nuclear factor-kappaB (NF-kappaB) activity stimulated by tumor necrosis factor-alpha (TNF-alpha), one of heparin binding cytokine ... The luciferase assay demonstrated that overexpression of EXTL3 enhanced TNF-alpha induced NF-kappaB activity ... The luciferase assay demonstrated that overexpression of EXTL3 enhanced TNF-alpha induced NF-kappaB activity ... However, EXTL3 did not affect the CD40 mediated NF-kappaB activation ... However, EXTL3 did not affect the CD40 mediated NF-kappaB activation ... These results suggest that EXTL3 may modulate NF-kappaB mediated by TNF-alpha
Qin et al., J Pharmacol Exp Ther 2001 : Prostaglandin A ( 1 ) ( PGA1 ) reportedly inhibits NF-kappaB activation and induces expression of heat shock proteins ... PGA1 ( 5-80 nmol ) significantly inhibited QA-induced NF-kappaB activation by blocking inhibitory kappaB-alpha degradation but had no effect on activator protein-1 binding activity
Gnad et al., Exp Cell Res 2001 : Here, we addressed the question of whether small GTPases of the Rho family are involved in the stimulation of NF-kappaB signaling by genotoxic agents or TNFalpha in HeLa cells ... This indicates that isoprenylated regulatory proteins participate in the regulation of NF-kappaB by DNA damaging agents as well as by TNFalpha ... Specific blockage of Rho signaling by Clostridium difficile toxin B attenuated UV- and doxorubicin induced activation of NF-kappaB, but did not affect stimulation of NF-kappaB by TNFalpha
Fahmi et al., Arthritis Rheum 2001 : Similarly, in a PPARgamma dependent manner, 15d-PGJ2 inhibited deltaMEKK-1 induced AP-1- and NF-kappaB-luciferase reporter plasmid activation
Azuma et al., Biochem Biophys Res Commun 2001 (Salivary Gland Neoplasms) : 5-Fluorouracil suppression of NF-KappaB is mediated by the inhibition of IKappab kinase activity in human salivary gland cancer cells
Wang et al., Respirology 2001 (Tuberculosis, Pulmonary) : An inhibition of NF-kappaB by pyrrolidine dithiocarbamate attenuated IL-1beta and TNF-alpha synthesis ... An inhibition of NF-kappaB by pyrrolidine dithiocarbamate attenuated IL-1beta and TNF-alpha synthesis
Hatano et al., Gastroenterology 2001 : NF-kappaB stimulates inducible nitric oxide synthase to protect mouse hepatocytes from TNF-alpha- and Fas mediated apoptosis ... Our aim was to study the role of NF-kappaB activation and inducible nitric oxide synthases ( iNOSs ) in TNF-alpha- and Fas mediated apoptosis in hepatocytes ... The IkappaB superrepressor and a dominant negative form of IkappaB kinase beta (IKKbeta) inhibited NF-kappaB binding activity by TNF-alpha or Jo2 and sensitized hepatocytes to TNF-alpha- and Jo2 mediated apoptosis ... TNF-alpha and Jo2 induced iNOS messenger RNA and protein levels through the induction of NF-kappaB ... Endogenous iNOS , which is activated by NF-kappaB via IKKbeta, provides partial protection from apoptosis
Bui et al., J Cell Biol 2001 : NGF induced NFkappaB activity and Bcl-xL expression were inhibited in cells overexpressing the NFkappaB inhibitor, IkappaBalpha ... Unlike tumor necrosis factor-alpha (TNF-alpha), however, NGF induced NFkappaB activation occurred without significant degradation of IkappaBs determined by Western blot analysis and time-lapse imaging of neurons expressing green fluorescent protein tagged IkappaBalpha ... Overexpression of a Y42F mutant of IkappaBalpha potently suppressed NFG-, but not TNF-alpha induced NFkappaB activation ... Conversely, overexpression of a dominant negative mutant of TNF receptor associated factor-6 blocked TNF-alpha-, but not NGF induced NFkappaB activation ... We conclude that NGF and TNF-alpha induce different signaling pathways in neurons to activate NFkappaB and bcl-x gene expression ... We conclude that NGF and TNF-alpha induce different signaling pathways in neurons to activate NFkappaB and bcl-x gene expression
Catania et al., Ann N Y Acad Sci 2000 : At the molecular level, alpha-MSH inhibited activation of the transcription factor NF-kappa B known to enhance HIV expression
Bergquist et al., Ann N Y Acad Sci 2000 : LPS induced binding of NF-kappa B to DNA, determined by electrophoretic mobility shift assay, was inhibited by extrinsic dopamine, leading to a decreased proliferation and cytokine expression
Nomura et al., Life Sci 2001 : Experiments using stable clones of rat C6 glioma cells transfected with dominant negative IkappaB alpha ( serines 32 and 36 replaced by alanine ) suggest that NF-kappaB activation ( phosphorylation of IkappaB alpha ) is involved in LPS/IFNgamma- or IL-1beta/IFNgamma induced iNOS expression ... Experiments using stable clones of rat C6 glioma cells transfected with dominant negative IkappaB alpha ( serines 32 and 36 replaced by alanine ) suggest that NF-kappaB activation ( phosphorylation of IkappaB alpha ) is involved in LPS/IFNgamma- or IL-1beta/IFNgamma induced iNOS expression ... Experiments using stable clones of rat C6 glioma cells transfected with dominant negative IkappaB alpha ( serines 32 and 36 replaced by alanine ) suggest that NF-kappaB activation ( phosphorylation of IkappaB alpha ) is involved in LPS/IFNgamma- or IL-1beta/IFNgamma induced iNOS expression ... Experiments using stable clones of rat C6 glioma cells transfected with dominant negative IkappaB alpha ( serines 32 and 36 replaced by alanine ) suggest that NF-kappaB activation ( phosphorylation of IkappaB alpha ) is involved in LPS/IFNgamma- or IL-1beta/IFNgamma induced iNOS expression
Blair et al., Diabetes 2001 : This study examines the role of nuclear factor-kappaB (NF-kappaB) and the dsRNA dependent protein kinase ( PKR ) in dsRNA + IFN-gamma induced nitric oxide synthase (iNOS) expression and nitric oxide production by rat, mouse, and human islets ... These results suggest that 1 ) NF-KB activation is required for dsRNA + IFN-gamma induced iNOS expression, 2 ) PKR is not required for either dsRNA induced NF-kappaB activation or dsRNA + IFN-y induced iNOS expression by islets, and 3 ) PKR is not required for dsRNA + IFN-gamma induced inhibition of glucose stimulated insulin secretion by islets ... These results suggest that 1 ) NF-KB activation is required for dsRNA + IFN-gamma induced iNOS expression, 2 ) PKR is not required for either dsRNA induced NF-kappaB activation or dsRNA + IFN-y induced iNOS expression by islets, and 3 ) PKR is not required for dsRNA + IFN-gamma induced inhibition of glucose stimulated insulin secretion by islets
Yoshiyama et al., J Mol Cell Cardiol 2001 (Myocardial Infarction) : ACE inhibitor and ARB inhibited JNKs, NF- kappa B and AP-1 activities
Lucio-Cazana et al., J Am Soc Nephrol 2001 : Experiments that used pharmacologic and genetic inhibitors showed that the IL-1beta-inducible MCP-1 expression was dependent on nuclear factor-kappaB (NF-kappaB) and independent of activator protein 1 (AP-1) ... Experiments that used pharmacologic and genetic inhibitors showed that the IL-1beta-inducible MCP-1 expression was dependent on nuclear factor-kappaB (NF-kappaB) and independent of activator protein 1 (AP-1) ... In contrast, the constitutive expression of MCP-1 was dependent on both NF-kappaB and AP-1 ... t-RA substantially inhibited the constitutive activity of AP-1 but did not inhibit NF-kappaB activity in mesangial cells ... These data suggested that ( 1 ) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and ( 2 ) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway ... These data suggested that ( 1 ) constitutive and IL-1beta-inducible expression of MCP-1 was differently regulated by AP-1 and NF-kappaB and ( 2 ) t-RA inhibited selectively the constitutive expression of MCP-1 via intervention in the AP-1 pathway
McKinsey et al., Mol Immunol 2000 : Transcription factor NF-kappaB regulates inducible CD83 gene expression in activated T lymphocytes
Katsuyama et al., J Biochem 2001 : Gel shift assay and an immunocytochemical study showed that N2733 inhibited IL-1beta induced NF-kappaB activation and its nuclear translocation ... Our results suggest that the inhibitory action of N2733 toward IL-1beta induced NF-kappaB activation and iNOS expression is due to its blockade of the upstream signal ( s ) leading to IKK-alpha activation, and subsequent phosphorylation and degradation of IkappaB-alpha in rat VSMCs
Roebuck et al., J Bone Miner Res 2001 (Osteosarcoma) : Protein tyrosine kinase ( PTK ) inhibitors, which significantly reduce the suppressive effect of titanium particles on collagen gene expression, inhibited NF-kappaB binding activity showing that titanium particle stimulation of PTK signals in osteoblasts are critical for both NF-kappaB activation and collagen gene expression
Luftig et al., J Biol Chem 2001 : Effects of the NIK aly mutation on NF-kappaB activation by the Epstein-Barr virus latent infection membrane protein, lymphotoxin beta receptor, and CD40 ... Effects of the NIK aly mutation on NF-kappaB activation by the Epstein-Barr virus latent infection membrane protein, lymphotoxin beta receptor , and CD40 ... We now find that NF-kappaB activation by Epstein-Barr virus latent membrane protein 1 (LMP1) or by an LMP1 transmembrane domain chimera with the LTbetaR signaling domain in human embryonic kidney 293 cells is selectively inhibited by a wild type dominant negative NIK comprised of amino acids 624-947 ( DN-NIK ) and not by aly DN-NIK ... LMP1, an LMP1 transmembrane domain chimera with the LTbetaR signaling domain, and LMP1/CD40 activate NF-kappaB in wt or aly murine embryo fibroblasts ... LMP1 , an LMP1 transmembrane domain chimera with the LTbetaR signaling domain, and LMP1/CD40 activate NF-kappaB in wt or aly murine embryo fibroblasts ... These data are compatible with a model in which activation of NF-kappaB by LMP1 and LTbetaR is mediated by an interaction of NIK or a NIK-like kinase with IKKalpha that is abrogated by the aly mutation ... On the other hand, CD40 mediates NF-kappaB activation through a kinase that interacts with a different component of the IKK complex
Suk et al., J Biol Chem 2001 (Necrosis...) : IFNgamma inhibits cytoprotective NF-kappa B through STAT1/IRF-1 pathways ... IFNgamma pretreatment attenuated TNFalpha- or p65 induced NF-kappaB reporter activity, whereas it did not inhibit p65 translocation or DNA binding of NF-kappaB ... IRF-1 transfection alone inhibited TNFalpha induced NF-kappaB activity, which was reversed by coactivator p300 overexpression
Koul et al., J Biol Chem 2001 : Tumor suppressor MMAC/PTEN inhibits cytokine induced NFkappaB activation without interfering with the IkappaB degradation pathway ... The ability of interleukin-1 (IL-1) to induce inhibitor ( IkappaB ) degradation or nuclear translocation of NFkappaB was, however, unaffected by MMAC/PTEN expression, suggesting that PI 3-kinase utilizes another equally important mechanism to control NFkappaB activation ... Moreover, IL-1 induced phosphorylation of p50 NFkappaB was potently inhibited in MMAC/PTEN expressing cells ... Taken together, our observations suggest that PI 3-kinase regulates NFkappaB activation through a novel phosphorylation dependent mechanism
Arnold et al., J Biol Chem 2001 : We also demonstrate that overexpression of a dominant negative IKKbeta blocks HPK1 mediated NFkappaB activation suggesting that HPK1 acts upstream of the IkappaB kinase complex
Cheng et al., J Biol Chem 2001 : Inhibition of nuclear factor-kappaB (NF-kappaB) activation by a trans-dominant inhibitory isoform of mutant IkappaBalpha did not affect ephrin A1 induction, suggesting that NF-kappaB proteins are not major regulators of ephrin A1 expression
Bertin et al., J Biol Chem 2001 : When expressed in cells, CARD11 and CARD14 activate NF-kappaB and induce the phosphorylation of BCL10 ... When expressed in cells, CARD11 and CARD14 activate NF-kappaB and induce the phosphorylation of BCL10 ... When expressed in cells, CARD11 and CARD14 activate NF-kappaB and induce the phosphorylation of BCL10
Yang et al., J Biol Chem 2001 : NF-kappaB activation requires the IFN dependent association of STAT3 with the IFNAR1 chain of the IFN receptor ... Whereas constitutively active PI-3K and Akt induce NF-kappaB activation, Ly294002 ( a PI-3K inhibitor ), dominant negative PI-3K, and kinase-dead Akt block IFN dependent NF-kappaB activation ... Whereas constitutively active PI-3K and Akt induce NF-kappaB activation, Ly294002 ( a PI-3K inhibitor ), dominant negative PI-3K, and kinase-dead Akt block IFN dependent NF-kappaB activation
Uranishi et al., J Biol Chem 2001 : TLS augmented NF-kappaB dependent promoter activity of the intercellular adhesion molecule-1 gene and interferon-beta gene
Castrillo et al., J Biol Chem 2001 : Specific effects on NF-kappa B-inducing kinase activity and on the coordinate activation of ERK and p38 MAPK ... Specific effects on NF-kappa B-inducing kinase activity and on the coordinate activation of ERK and p38 MAPK ... Transient expression of NF-kappaB inducing kinase (NIK) activated the IKK complex and NF-kappaB, a process that was inhibited by kaurenes, indicating that the inhibition of NIK was one of the targets of these diterpenes ... The absence of a coordinate activation of MAPK and IKK might contribute to a deficient activation of NF-kappaB that is involved in the anti-inflammatory activity of these molecules ... The absence of a coordinate activation of MAPK and IKK might contribute to a deficient activation of NF-kappaB that is involved in the anti-inflammatory activity of these molecules
Towne et al., J Biol Chem 2001 (Edema...) : These data show that TNF-alpha decreases AQP5 mRNA and protein expression and that the molecular pathway for this effect involves TNFR1 and activated NF-kappaB
Zapata et al., J Biol Chem 2001 : Analysis of various MUL and USP7 mutants by transient transfection assays indicated that the TDs of these proteins are necessary and sufficient for suppressing NF-kappaB induction by TRAF2 and TRAF6 as well as certain TRAF binding TNF family receptors ... Analysis of various MUL and USP7 mutants by transient transfection assays indicated that the TDs of these proteins are necessary and sufficient for suppressing NF-kappaB induction by TRAF2 and TRAF6 as well as certain TRAF binding TNF family receptors
Seitz et al., J Biol Chem 2001 : Overexpression of the icp75TNFR cDNA results in TNF induced activation of NFkappaB in a TNF receptor associated factor 2 (TRAF2) dependent manner ... Overexpression of the icp75TNFR cDNA results in TNF induced activation of NFkappaB in a TNF receptor associated factor 2 (TRAF2) dependent manner
Dikov et al., Cancer Res 2001 : VEGF effectively blocked TNF-alpha induced NF-kappaB activation in HPCs from RelB-/- mice, however, similar to the effect observed in HPCs obtained from RelB+/- and RelB+/+ mice ... VEGF effectively blocked TNF-alpha induced NF-kappaB activation in HPCs from RelB-/- mice, however, similar to the effect observed in HPCs obtained from RelB+/- and RelB+/+ mice ... Thus, VEGF appears to inhibit TNF-alpha induced NF-kappaB activation by VEGFR kinase independent inhibition of IKK
Reyes-Reyes et al., J Cell Sci 2001 (Leukemia) : Phosphatidylinositol 3-kinase mediates integrin dependent NF-kappaB and MAPK activation through separate signaling pathways ... We found that integrins activated both NF-kappaB and MAPK in a PI 3-K dependent manner, as wortmannin and LY294002 blocked these responses ... In addition, a dominant negative form of Rho augmented NF-kappaB activation and blocked MAPK activation, implying that these two pathways are in competition with each other
Wollmer et al., J Neuroimmunol 2001 : ATP or adenosine, but not ACM alone, also prevented the formation of a flat, amoeboid morphology induced by lipopolysaccharide (LPS) ; however, at 0.6-1 mM they did not reduce the initial LPS induced activation of the transcription factor NF-kappaB
Pype et al., Am J Respir Crit Care Med 2001 (Bronchial Hyperreactivity) : An inhibitor of NF-kappaB activation, pyrrolidine dithiocarbamate, and a p38 MAPK inhibitor, blocked the IL-1beta induced H(1)R desensitization, whereas anisomycin, an SAPK/JNK and p38 MAPK activator, mimicked the effect of IL-1beta ... Here, we have demonstrated that IL-1beta desensitizes H(1)R , which involves the activation of p38 MAPK and NF-kappaB , leading to the expression of cox-2 and the synthesis of PGE ( 2 )
Ravi et al., Nat Cell Biol 2001 : Inhibition of NF-kappaB by blocking activation of the IkappaB kinase complex reduces Bcl-x L expression and sensitizes tumour cells to TRAIL induced apoptosis
Xu et al., Biochem J 2001 : Nuclear factor-kappaB (NF-kappaB) , a previously identified positive regulator of MMP-1 expression induced by fl-coll [ Xu, Zutter, Santoro and Clark ( 1998 ) J. Cell Biol. 140, 709-719 ] was mediated by fl-coll activated p38alpha ... Nuclear factor-kappaB (NF-kappaB) , a previously identified positive regulator of MMP-1 expression induced by fl-coll [ Xu, Zutter, Santoro and Clark ( 1998 ) J. Cell Biol. 140, 709-719 ] was mediated by fl-coll activated p38alpha
Opitz et al., J Biol Chem 2001 : Toll-like receptor-2 mediates Treponema glycolipid and lipoteichoic acid induced NF-kappaB translocation
Li et al., Proc Natl Acad Sci U S A 2001 : IL-1 induced NFkappa B and c-Jun N-terminal kinase (JNK) activation diverge at IL-1 receptor associated kinase (IRAK) ... Mutant I1A cells, lacking IL-1 receptor associated kinase (IRAK) mRNA and protein, have been used to study the involvement of IRAK in NFkappaB and c-Jun N-terminal kinase (JNK) activation ... Both the N-terminal death domain and the C-terminal region of IRAK are required for IL-1 induced NFkappaB and JNK activation, whereas the N-proximal undetermined domain is required for the activation of NFkappaB but not JNK ... The phosphorylation and ubiquitination of IRAK deletion mutants correlate tightly with their ability to activate NFkappaB in response to IL-1 , but IRAK can mediate IL-1 induced JNK activation without being phosphorylated
Fortenberry et al., Inflamm Res 2001 : S-nitrosoglutathione inhibits TNF-alpha induced NFkappaB activation in neutrophils ... TNFalpha increased nuclear NFkappaB activity compared to unstimulated neutrophils ( p < 0.001, n = 5 ) ... GSNO ( 500 microM ) decreased TNFalpha induced NFkappaB activity ( p < 0.05 ) and inhibited NFkappaB activity whether given prior to or during TNFalpha exposure
Shikhman et al., J Immunol 2001 : N-acetylglucosamine mediated inhibition of the IL-1beta response of human chondrocytes was not associated with the decreased inhibition of the mitogen activated protein kinases c-Jun N-terminal kinase, extracellular signal related kinase, and p38 , nor with activation of the transcription factor NF-kappaB
Helenius et al., Antioxid Redox Signal 2001 : In addition, the level of Ser32 phosphorylated I kappa B alpha was unaffected by age, suggesting that neither the IKK complex nor altered level of the main inhibitors is involved in the observed up-regulation of NF-kappa B binding activity ... The up-regulation of constitutive nuclear NF-kappa B binding activity and increased levels of nuclear p52 and p65 proteins might affect the expression of some NF-kappa B target genes in the aging liver ... The up-regulation of constitutive nuclear NF-kappa B binding activity and increased levels of nuclear p52 and p65 proteins might affect the expression of some NF-kappa B target genes in the aging liver
Ho et al., Phytomedicine 2001 (Arteriosclerosis) : Oxidation of low-density lipoprotein (LDL) and activation of the pleiotropic transcription factor nuclear factor kappa B (NF-kappaB) , are often the chemical and molecular alterations associated with the development of the atherosclerotic lesion
Lancaster et al., Inflammation 2001 (Endotoxemia...) : Calpain-1 inhibitor (CI-1) blocks activation of NF-kappaB by preventing proteolysis of the inhibitory protein IkappaB-alpha by the ubiquitin/proteasome pathway ... Treatment with Cl-1 prevented degradation of IkappaB-alpha and activation of NF-kappaB in the liver in response to LPS ; however, Cl-1 treatment had no detected effect on NF-kappaB activation in lung tissue ... Treatment with Cl-1 prevented degradation of IkappaB-alpha and activation of NF-kappaB in the liver in response to LPS ; however, Cl-1 treatment had no detected effect on NF-kappaB activation in lung tissue
Neuzil et al., Biochemistry 2001 (Inflammation) : Our data infer a pathway by which caspase activation in EC inhibits NF kappa B-dependent inflammatory activation and monocyte recruitment, and provide evidence for a relationship between pro-apoptotic and anti-inflammatory pathways
Kim et al., Clin Exp Immunol 2001 : Polarized secretion of CXC chemokines by human intestinal epithelial cells in response to Bacteroides fragilis enterotoxin : NF-kappa B plays a major role in the regulation of IL-8 expression
Kang et al., Toxicol Appl Pharmacol 2001 : The objective of this study was to determine whether ZnTMPyP exhibited the ability to inhibit the production of reactive oxygen species ( ROS ), the activation of NF-kappaB , or the secretion of IL-1 in RAW 264.7 cells, and whether such inhibitory activity was related to the ROS scavenging ability of ZnTMPyP
Milligan et al., Anticancer Res 2001 (Adenocarcinoma...) : TNF induced I kappa B proteolysis and NF-kappa B nuclear translocation ; however, this was blocked by pretreatment with proteasome inhibitors
Koo et al., Planta Med 2001 : A sesquiterpene lactone, costunolide ( CTN ), was identified from Magnolia grandiflora together with parthenolide (PTN) by its strong inhibition of LPS induced NF-kappa B activation ... CTN also significantly inhibited LPS induced DNA binding activity of NF-kappa B as well as the LPS induced degradation of I kappa B-alpha and -beta
Herring et al., Cell Signal 2001 (MAP Kinase Signaling System) : Therefore, the objective of the present studies was to determine the role of cAMP and protein kinase A (PKA) in the CBN mediated inhibition of IL-2, CREB, and NF-kappaB in PMA/Io activated thymocytes
Muenzner et al., J Biol Chem 2001 : Gonococcal infection activated a nuclear factor-kappaB (NF-kappaB) heterodimer consisting of p50 and p65, and inhibitors that prevent the nuclear translocation of activated NF-kappaB complex inhibited CEACAM1 transcript expression ... Together, our results support a model whereby the outer membrane `` blebs '' that are actively released by gonococci trigger a Toll-like receptor-4 dependent activation of NF-kappaB , which up-regulates the expression of CEACAM1 to allow Opa ( 52 ) -mediated neisserial binding ... The regulation of CEACAM1 expression by NF-kappaB also implies a broader role for this receptor in the general inflammatory response to infection
Steiner et al., Eur Urol 2001 (Carcinoma, Renal Cell) : NF-kB signaling induced by TNFalpha is suppressed by IFNalpha and IFNgamma in the permanent cell lines and in the primary RCC tumor cell cultures ... NF-kB signaling induced by TNFalpha is suppressed by IFNalpha and IFNgamma in the permanent cell lines and in the primary RCC tumor cell cultures ... In an in vitro ICT model we show that pretreatment of RCC with IL-2 and IFNalpha leads to a diminished NF-kB response to TNFalpha
Glazner et al., J Biol Chem 2001 : Activation of NF-kappaB in response to tumor necrosis factor-alpha and glutamate was completely abolished when IP ( 3 ) receptors were blocked, and NF-kappaB activation in response to depletion of ER calcium by thapsigargin treatment was also decreased by IP ( 3 ) receptor blockade
Lim et al., Lab Invest 2001 (Adenocarcinoma...) : We investigated the role of NF-kappaB in COX-2 expression and cell proliferation in human gastric cancer AGS cells ... In conclusion, NF-kappaB mediates COX-2 expression, which may be related to cell proliferation, in human gastric cancer cells
Wheeler et al., J Leukoc Biol 2001 (Liver Diseases) : These data support the hypothesis that LPS induced NF-kappaB activation and TNF-alpha production in Kupffer cells are oxidant dependent
Won et al., Brain Res Mol Brain Res 2001 (Glioma) : In C6 glioma cells, the stimulation with lipopolysaccharide ( LPS ; 1 microg/ml ) evoked increases of nitric oxide ( NO ) production, NO synthase ( iNOS ) mRNA expression, phosphorylation of p38 mitogen activated protein kinase ( p-p38 ), and the activation of NF kappa B ... In C6 glioma cells, the stimulation with lipopolysaccharide ( LPS ; 1 microg/ml ) evoked increases of nitric oxide ( NO ) production, NO synthase ( iNOS ) mRNA expression, phosphorylation of p38 mitogen activated protein kinase ( p-p38 ), and the activation of NF kappa B ... Furthermore, LPS induced increases of p-p38, but not activation of NF kappa B , were also reduced by FSK and H89 reversed the FSK induced inhibition response ... However, the activation of NF kappa B by LPS also not affected by SB203580
Bachis et al., J Neurosci 2001 : IL-10 blocked both the glutamate mediated induction of caspase-3 as well as NF-kappaB DNA binding activity, suggesting that the neuroprotective properties of IL-10 may rely on its ability to block the activity of proapoptotic proteins
Frost et al., Toxicol Appl Pharmacol 2001 : Nuclear factor-kappaB (NF-kappaB) tightly regulates TNF-alpha transcription
Franco et al., J Immunol 2001 (Melanoma) : The role of NF-kappa B in TNF related apoptosis inducing ligand ( TRAIL ) -induced apoptosis of melanoma cells ... It is also known that TNF related apoptosis inducing ligand ( TRAIL ) can activate NF-kappaB through the death receptors TRAIL-R1 and TRAIL-R2, and decoy receptor TRAIL-R4 ... Furthermore, studies with NF-kappaB reporter constructs revealed that the resistance of melanoma lines to TRAIL induced apoptosis was correlated to activation of NF-kappaB in response to TRAIL ... Therefore, these results suggest that activation of NF-kappaB by TRAIL plays an important role in resistance of melanoma cells to TRAIL induced apoptosis and further suggest that inhibitors of NF-kappaB may be useful adjuncts in clinical use of TRAIL against melanoma
Hsu et al., J Immunol 2001 : The nuclear translocation and DNA binding study revealed that ceramide can inhibit LPS induced NF-kappaB and AP-1 activation ... In conclusion, we suggest that ceramide inhibition of LPS mediated induction of inducible NO synthase and cyclooxygenase-2 is due to reduction of the activation of NF-kappaB and AP-1, which might result from ceramide 's inhibition of LPS stimulated IkappaB kinase, p38 mitogen activated protein kinase, and protein kinase C ... In conclusion, we suggest that ceramide inhibition of LPS mediated induction of inducible NO synthase and cyclooxygenase-2 is due to reduction of the activation of NF-kappaB and AP-1, which might result from ceramide 's inhibition of LPS stimulated IkappaB kinase, p38 mitogen activated protein kinase, and protein kinase C
Herndon et al., J Immunol 2001 : ZAP-70 and SLP-76 regulate protein kinase C-theta and NF-kappa B activation in response to engagement of CD3 and CD28 ... ZAP-70 and SLP-76 regulate protein kinase C-theta and NF-kappa B activation in response to engagement of CD3 and CD28 ... ZAP-70 and SLP-76 regulate protein kinase C-theta and NF-kappa B activation in response to engagement of CD3 and CD28 ... Reexpression of ZAP-70 restored PKCtheta and NF-kappaB activation in response to TCR and CD28 coengagement ... Wild-type SLP-76 fully restored PKCtheta and NF-kappaB activation in the SLP-76 negative cells, whereas 3YF-SLP-76, which lacks the sites of tyrosine phosphorylation required for Vav-1 binding, only partially rescued signaling ... These data illustrate the importance of the ZAP-70/SLP-76 signaling pathway in CD3/CD28 stimulated activation of PKC theta and NF-kappaB , and suggest that Vav-1 association with SLP-76 may be important in this pathway
Hay et al., Mol Cell Biol 2001 : Transcriptional activation of NF-kappaB is mediated by signal induced phosphorylation and degradation of its inhibitor, IkappaBalpha ... NF-kappaB activation induces a rapid resynthesis of IkappaBalpha which is responsible for postinduction repression of transcription
Mitin et al., Oncogene 2001 : Expression of IkappaBalpha SR, an inhibitor of NFkappaB , does not reverse the differentiation-defective phenotype of Ras expressing cultures, but does induce differentiation in cultures treated with tumor necrosis factor ( TNFalpha ) or in cultures expressing the RelA/p65 subunit of NFkappaB
Pianetti et al., Oncogene 2001 (Breast Neoplasms...) : Overexpression of Her-2/neu and EGFR-4 in Ba/F3 cells led to constitutive PI3- and Akt kinase activities, and induction of classical NF-kappaB ( p50/p65 ) ... Overexpression of Her-2/neu and EGFR-4 in Ba/F3 cells led to constitutive PI3- and Akt kinase activities, and induction of classical NF-kappaB ( p50/p65 )
Ishii et al., Oncogene 2001 (Cell Transformation, Neoplastic) : For example, PKR induces I kappa B alpha kinase (IKK) activity and I kappa B alpha phosphorylation leading to the induction of NF-kappa B-mediated gene transcription ... For example, PKR induces I kappa B alpha kinase (IKK) activity and I kappa B alpha phosphorylation leading to the induction of NF-kappa B-mediated gene transcription ... For example, PKR induces I kappa B alpha kinase (IKK) activity and I kappa B alpha phosphorylation leading to the induction of NF-kappa B-mediated gene transcription ... Recent findings suggested that NF-kappa B activation by PKR does not require the catalytic activity of the kinase ... We also show that the effects of PKR mutants on IKK and NF-kappa B activation are independent of cell transformation but are dependent on the amount of the mutant PKR proteins expressed in cells
Gil et al., Oncogene 2001 : We have previously shown that induction of NF-kappaB by PKR is involved in apoptosis commitment and this process is mediated through activation of the IKK complex ... To gain insights into the mechanism of activation of NF-kappaB by PKR, we have analysed the domains of PKR involved in IKK activation and subsequent NF-kappaB induction ... To gain insights into the mechanism of activation of NF-kappaB by PKR , we have analysed the domains of PKR involved in IKK activation and subsequent NF-kappaB induction ... An inactive PKR mutant ( K296R ), was unable to induce NF-kappaB activation despite full expression of the protein in a wide range of concentrations, as defined by Western blot, EMSA, IKK kinase activity and NF-kappaB transactivation assays ... Moreover, the mutant PKR ( K296R ) acts as a dominant negative of PKR induced eIF-2alpha phosphorylation and NF-kappaB activation
Bartke et al., Oncogene 2001 (Colonic Neoplasms) : Moreover, induction of p53 interfered with TNF induced NF-kappaB activation independently from apoptosis-induction
Yang et al., Oncogene 2001 (Cell Transformation, Neoplastic) : A novel transformation suppressor, Pdcd4, inhibits AP-1 transactivation but not NF-kappaB or ODC transactivation
Lallemand et al., Oncogene 2001 : Interestingly, the stable expression of oncogenic Ras in MDCK cells which suppressed Smad7 inhibition of NF-kappaB also suppressed Smad7 potentiation of serum withdrawal induced apoptosis and anoikis ... In addition, Smad7 inhibited TNF-alpha stimulation of NF-kappaB and increased TNF-alpha mediated apoptosis in MDCK cells
Russo et al., Int J Radiat Oncol Biol Phys 2001 (Colorectal Neoplasms) : NF-kappaB is activated by tumor necrosis factor , certain chemotherapeutic agents, and ionizing radiation, leading to inhibition of apoptosis
Iwamura et al., Genes Cells 2001 : Although the induction of NF-kappaB by dsRNA/virus and TNF-alpha involves common downstream pathways including IKK activation, the upstream, Geldanamycin-sensitive process was unique to the dsRNA/virus induced signal
Qiu et al., J Neurosci Res 2001 (Hypoxia, Brain) : Differential NF-kappa B regulation of bcl-x gene expression in hippocampus and basal forebrain in response to hypoxia ... The transcription factor NF-kappa B regulates bcl-x gene expression ... In this study, we examined the role of NF-kappa B in the regulation of bcl-x in hypoxia induced cell death
Andrews et al., J Surg Res 2001 (Neoplasm Metastasis...) : LPS increased tumor cell NF-kappaB activation
Pugh et al., Immunopharmacol Immunotoxicol 2001 : However, podophyllotoxin ( 0.1 microM ) enhanced LPS induced NF-kappa B activation and IL-1beta mRNA expression between 2 and 3-fold
Funakoshi et al., Biochem Biophys Res Commun 2001 (Glioblastoma) : A MEK inhibitor, PD98059 enhances IL-1 induced NF-kappaB activation by the enhanced and sustained degradation of IkappaBalpha ... In a human glioblastoma cell line, T98G, IL-1 induced NF-kappaB activation was significantly augmented by the pretreatment of a specific MEK inhibitor, PD98059 ... In contrast, ectopic expression of a constitutive activated form of Raf ( v-Raf ) reduced IL-1 induced NF-kappaB activation , and this inhibition was completely reversed by PD98059 ... Interestingly, PD98059 sustained IL-1 induced NF-kappaB DNA binding activity by an electrophoretic mobility shift assay and also IkappaBalpha degradation, presumably by augmenting and sustaining the proteasome activation
Suk et al., FEBS Lett 2001 (Uterine Cervical Neoplasms) : We found that ( i ) IFNalpha/TNFalpha synergistically induced apoptosis of ME-180 cells, which was accompanied by activation of caspases-3 and -8 ; ( ii ) IFNalpha induced signal transducer and activator of transcription ( STAT ) 1 phosphorylation, and transfection of phosphorylation-defective STAT1 dominant negative mutant inhibited IFNalpha/TNFalpha induced apoptosis ; ( iii ) inhibition of nuclear factor kappaB (NF-kappaB) by proteasome inhibitor MG-132 sensitized ME-180 cells to TNFalpha alone ; ( iv ) IFNalpha treatment attenuated TNFalpha induced NF-kappaB reporter activity, while it did not inhibit DNA binding of NF-kappaB
Aifantis et al., Nat Immunol 2001 (Calcium Signaling) : Constitutive pre-TCR signaling promotes differentiation through Ca2+ mobilization and activation of NF-kappaB and NFAT
Ghaleh et al., J Soc Biol 2000 (Myocardial Infarction...) : This leads to the translocation of protein kinase C. Secondary, the activation of the tyrosine kinases pathway and the transcriptional factor NF kappa B induces iNOS
Guo et al., Acta Pharmacol Sin 2000 : To study the inhibitory effects of antisense interleukin-1 receptor associated kinase-2 ( IRAK-2 ) oligodeoxynucleotide ( ODN ) on interleukin-1 (IL-1) stimulated nuclear factor-kappa B (NF-kappa B) activation ... ( 2 ) Antisense IRAK-2 ODN inhibited IL-1 induced NF-kappa B activation in a concentration ( 1-8 micrograms ) - and time ( 5-24 h ) -dependent manner ... ( 2 ) Antisense IRAK-2 ODN inhibited IL-1 induced NF-kappa B activation in a concentration ( 1-8 micrograms ) - and time ( 5-24 h ) -dependent manner ... Antisense IRAK-2 ODN inhibited IL-1 induced NF-kappa B activation ... Antisense IRAK-2 ODN inhibited IL-1 induced NF-kappa B activation
Guo et al., Acta Pharmacol Sin 2000 (Carcinoma, Hepatocellular...) : To investigate whether phosphatidylinositol (PI) 3-kinase is involved in interleukin-18 (IL-18) induced nuclear factor-kappa B (NF-kappa B) activation ... To investigate whether phosphatidylinositol (PI) 3-kinase is involved in interleukin-18 (IL-18) induced nuclear factor-kappa B (NF-kappa B) activation ... ( 2 ) IL-18 activated NF-kappa B from basal level of 0.153 +/- 0.008 to 1.942 +/- 0.017 ... ( 3 ) Antisense PI 3-kinase ODN inhibited IL-18 induced NF-kappa B activation in a concentration ( 1-8 mg/L ) - and time ( 5-24 h ) -dependent fashion ... ( 3 ) Antisense PI 3-kinase ODN inhibited IL-18 induced NF-kappa B activation in a concentration ( 1-8 mg/L ) - and time ( 5-24 h ) -dependent fashion ... PI 3-kinase is necessary for IL-18 stimulated NF-kappa B activation ... PI 3-kinase is necessary for IL-18 stimulated NF-kappa B activation
Haddad et al., Br J Pharmacol 2001 : In addition, Y-40138 blockaded, in a dose dependent manner, the LPS induced nuclear activation of NF-kappa B
D'Addario et al., J Mol Biol 2001 : Epstein-Barr Virus and its glycoprotein-350 upregulate IL-6 in human B-lymphocytes via CD21, involving activation of NF-kappaB and different signaling pathways ... To our knowledge this is the first report showing that the binding of a herpesvirus envelope glycoprotein to CR2 on human B cells results in the activation of the NF-kappaB transcription factor leading to the upregulation of IL-6 gene expression in these lymphocytes ... To our knowledge this is the first report showing that the binding of a herpesvirus envelope glycoprotein to CR2 on human B cells results in the activation of the NF-kappaB transcription factor leading to the upregulation of IL-6 gene expression in these lymphocytes
Marshall et al., Biochemistry 2001 : We observed that both S-nitrosocysteine and cytokine activated NOS2 inhibited NF-kappaB in human respiratory cells or murine macrophages
Wu et al., Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2001 : and Their time courses were similar, thus suggesting that the induction of IL-8 gene expression by TNF-alpha is probably due to the activation of NF-kappa B
Chen et al., Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2001 : The induction of IL-8 gene expression by laminar shear stress is probably due to the activation of NF-kappa B
Han et al., Diabetes 2001 : Finally, we evaluated the effect of TIMP-1 on inducible nitric oxide synthase (iNOS) gene expression and nuclear factor (NF)-kappaB activity in INS-1 cells stimulated with high-dose cytokines
Huang et al., Eur J Pharmacol 2001 : These results suggest that 7HQ derivatives exhibit inhibitory effects on lipopolysaccharide induced nitric oxide production and expression of inducible nitric oxide synthase and cyclooxygenase-2 through inhibition of IkappaB-alpha degradation and NF-kappaB activation
Rogler et al., Scand J Gastroenterol 2001 (Colitis, Ulcerative...) : TNF and IL-1 induced NF-kappaB activation ... TNF and IL-1 induced NF-kappaB activation ... The induction of IL-8, M-CSF and GM-CSF, but not of IL-6 secretion, is mediated mainly by NF-kappaB activation ... The induction of IL-8, M-CSF and GM-CSF , but not of IL-6 secretion, is mediated mainly by NF-kappaB activation ... The induction of IL-8 , M-CSF and GM-CSF, but not of IL-6 secretion, is mediated mainly by NF-kappaB activation
Zhu et al., Virology 2001 : However, NF kappa B activation by TNF was not significantly altered by the HCV core protein, suggesting the existence of TRAF2 independent pathways for NF kappa B activation
Van Bezooijen et al., Bone 2001 (Bone Resorption) : To further examine the pathway by which osteoclastic resorption was stimulated, we used osteoprotegerin , a specific inhibitor of the receptor activator of NF-kappaB ( RANK ) /receptor activator of the NF-kappaB ligand ( RANKL ) pathway
Chanson et al., Am J Pathol 2001 (Cystic Fibrosis) : TNF-alpha induced maximal translocation of NF-kappaB into the nucleus of non-CF as well as CF airway cells within 20 minutes
Hirota et al., J Biol Chem 2001 : We have demonstrated that overexpression of the EGF receptor (EGFR) in NIH3T3 cells significantly enhances TNF induced NF-kappa B-dependent luciferase activity even without EGF, that EGF treatment has a synergistic effect on the induction of the reporter activity, and that this enhancement is suppressed by AG1478, EGFR-specific tyrosine kinase inhibitor ... We have demonstrated that overexpression of the EGF receptor (EGFR) in NIH3T3 cells significantly enhances TNF induced NF-kappa B-dependent luciferase activity even without EGF, that EGF treatment has a synergistic effect on the induction of the reporter activity, and that this enhancement is suppressed by AG1478, EGFR-specific tyrosine kinase inhibitor ... Taken together, this evidence strongly suggests that EGFR transactivation by TNF, which is regulated in a redox dependent manner, is playing a pivotal role in TNF induced NF-kappa B activation ... Taken together, this evidence strongly suggests that EGFR transactivation by TNF, which is regulated in a redox dependent manner, is playing a pivotal role in TNF induced NF-kappa B activation
Cammarano et al., J Biol Chem 2001 : Here we show that although Dbl activation of NF kappa B requires Cdc42, Rac, and Rho , the different GTPases activate NF kappa B by different mechanisms ... Here we show that although Dbl activation of NF kappa B requires Cdc42 , Rac, and Rho, the different GTPases activate NF kappa B by different mechanisms ... Here we show that although Dbl activation of NF kappa B requires Cdc42, Rac , and Rho, the different GTPases activate NF kappa B by different mechanisms ... Whereas Rac stimulates the activity of the I kappa B kinase IKK beta, Cdc42 and Rho activate NF kappa B without activating either IKK alpha or IKK beta ... Whereas Rac stimulates the activity of the I kappa B kinase IKK beta, Cdc42 and Rho activate NF kappa B without activating either IKK alpha or IKK beta
Choi et al., J Biol Chem 2001 : Here we show that antisense oligonucleotide induced inhibition of FLASH expression abolished TNF-alpha induced activation of NF-kappaB in HEK293 cells, as determined by luciferase reporter gene expression driven by a NF-kappaB responsive promoter
Schotte et al., J Biol Chem 2001 (Neoplasms) : TNF induced activation of NF-kappa B-dependent gene expression is not modulated by LiCl treatment
Deb et al., J Immunol 2001 : RNA dependent protein kinase PKR is required for activation of NF-kappa B by IFN-gamma in a STAT1 independent pathway ... RNA dependent protein kinase PKR is required for activation of NF-kappa B by IFN-gamma in a STAT1 independent pathway ... Here we show that IFN-gamma alone can activate NF-kappaB , by a Janus kinase-1 mediated, but Stat1 independent, mechanism ... NF-kappaB activation by IFN-gamma is associated with degradation of IkappaB beta
Pittet et al., J Immunol 2001 (Shock, Hemorrhagic) : In summary, these results provide the first in vivo evidence that NO, released within the airspaces of the lung probably secondary to the NF-kappaB dependent activation of iNOS , is a major proximal inflammatory mediator that limits the rate of alveolar epithelial transport after prolonged hemorrhagic shock by directly impairing the function of membrane proteins involved in the beta-adrenergic receptor-cAMP signaling pathway in alveolar epithelium
Corsini et al., Eur J Pharmacol 2001 : The binding of lipopolysaccharide to its receptor CD14 activates protein kinase C and nuclear factor-kappaB (NF-kappaB)
Langen et al., FASEB J 2001 (Cachexia) : TNF-alpha activated nuclear factor-kappaB (NF-kappaB) and interfered with the expression of muscle proteins in differentiating myoblasts ... Conversely, activation of NF-kappaB by overexpression of IkappaB kinase was sufficient to block myogenesis, illustrating the causal link between NF-kappaB activation and inhibition of myogenic differentiation
You et al., J Biol Chem 2001 : The inhibition of NF-kappa B by I kappa B alpha , a natural inhibitor of NF-kappa B , suppressed NDED mRNA expression induced by TNF ... The inhibition of NF-kappa B by I kappa B alpha, a natural inhibitor of NF-kappa B, suppressed NDED mRNA expression induced by TNF
Huang et al., Life Sci 2001 (Shock, Septic) : DCDC significantly inhibited LPS evoked degradation of IkappaB-alpha and the nuclear translocation of NF-kappaB ; it also exhibited the activity of scavenging the stable free radical 1,1-diphenyl-2-picrylhydrazyl ( DPPH ) ... Taken together, we demonstrate that DCDC exhibits inhibitory effects on nitric oxide production through the inhibition of IkappaB-alpha degradation and NF-kappaB activation , and therefore the suppression of iNOS expression ... Taken together, we demonstrate that DCDC exhibits inhibitory effects on nitric oxide production through the inhibition of IkappaB-alpha degradation and NF-kappaB activation , and therefore the suppression of iNOS expression
You et al., Zhonghua Gan Zang Bing Za Zhi 2001 : To investigate the effect of Cpd 861 on nuclear factor-kappa B (NF-kappa B) binding activity of hepatic stellate cells (HSC) in vitro ... Cpd 861 suppressed the binding activity of NF-kappa B in HSCs compared with the control group
Dokka et al., Am J Physiol Lung Cell Mol Physiol 2001 : Although still controversial, IL-10 has been shown to inhibit NF-kappaB activation through a process that involves proteolytic degradation of inhibitory subunit IkappaB-alpha
Lee et al., Dev Biol 2001 : In 10.5 day limb cells, gas1 overexpression had little effect on Ap-1, NFkappaB , and c-myc activities ... In contrast, gas1 overexpression in 12.5 day limb cells enhanced AP-1 response while it inhibited NFkappaB and c-myc activities
Gaide et al., FEBS Lett 2001 (Lymphoma) : Carma1 , a CARD containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation ... Bcl10 , a caspase recruitment domain ( CARD ) -containing protein identified from a breakpoint in mucosa associated lymphoid tissue ( MALT ) B lymphomas, is essential for antigen-receptor mediated nuclear factor kappaB (NF-kappaB) activation in lymphocytes ... Moreover, expression of Carma1 induces phosphorylation of Bcl10 and activation of the transcription factor NF-kappaB ... Moreover, expression of Carma1 induces phosphorylation of Bcl10 and activation of the transcription factor NF-kappaB
Zouki et al., J Leukoc Biol 2001 : The NO synthase inhibitors aminoguanidine and N ( G ) -nitro-L-arginine methyl ester blocked nuclear accumulation of activator protein-1 (AP-1) and nuclear factor (NF)-kappaB in both polymorphonuclear ( PMN ) and mononuclear leukocytes and inhibited IL-8 mRNA expression and IL-8 release by approximately 90 % in response to IL-1beta and TNF-alpha
Descamps et al., J Biol Chem 2001 (Breast Neoplasms) : In contrast, the anti-apoptotic effect of NGF ( studied using the ceramide analogue C2 ) required p75 ( NTR ) as well as the activation of the transcription factor NF-kB , but neither p140(trkA) nor MAPK was necessary
Donepudi et al., J Immunol 2001 (Mast-Cell Sarcoma) : Moreover, exposure of P815 tumor cells to a cell-permeable peptide that selectively inhibits NF-kappaB activation by blocking the activation of the IkappaB-kinase complex was found to inhibit the L-PAM induced B7-1 mRNA accumulation, indicating that NF-kappaB activation is essential for the L-PAM induced B7-1 gene expression
Schwabe et al., J Immunol 2001 : CD40 activates NF-kappa B and c-Jun N-terminal kinase and enhances chemokine secretion on activated human hepatic stellate cells ... Stimulation of CD40 with CD40 ligand (CD40L) transfected baby hamster kidney cells induces NF-kappaB , as demonstrated by the activation of I-kappaB kinase (IKK), increased NF-kappaB DNA binding, and p65 nuclear translocation
Yang et al., J Immunol 2001 : The involvement of a conventional PKC isoform was confirmed by the finding that expression of PKCalpha enhanced the effect of Galpha(16), and a dominant negative PKCalpha partially blocked Galpha(16) mediated NF-kappaB activation ... The involvement of a conventional PKC isoform was confirmed by the finding that expression of PKCalpha enhanced the effect of Galpha(16), and a dominant negative PKCalpha partially blocked Galpha(16) mediated NF-kappaB activation ... In addition to formyl peptide receptor, Galpha(16) also enhanced NF-kappaB activation by the C5a and C3a receptors, and by CXC chemokine receptor 2 and CCR8 ... In addition to formyl peptide receptor, Galpha(16) also enhanced NF-kappaB activation by the C5a and C3a receptors, and by CXC chemokine receptor 2 and CCR8 ... In addition to formyl peptide receptor, Galpha(16) also enhanced NF-kappaB activation by the C5a and C3a receptors, and by CXC chemokine receptor 2 and CCR8
Kreuz et al., Mol Cell Biol 2001 : We found that cFLIP can be upregulated in some cell lines under critical involvement of the NF-kappaB pathway, but NF-kappaB activation was clearly not sufficient for cFLIP induction in all cell lines ... Treatment of SV80 cells with the proteasome inhibitor N-benzoyloxycarbonyl ( Z ) -Leu-Leu-leucinal ( MG-132 ) or geldanamycin, a drug interfering with tumor necrosis factor (TNF) induced NF-kappaB activation, inhibited TNF induced upregulation of cFLIP ... These data point to an important role for NF-kappaB in the regulation of the cFLIP gene
Brantley et al., Mol Biol Cell 2001 : We investigated the function of NF-kappaB during murine early postnatal mammary gland development by observing the consequences of increased NF-kappaB activity in mouse mammary epithelium lacking the gene encoding IkappaBalpha , a major inhibitor of NF-kappaB
Nozaki et al., Oncogene 2001 (Breast Neoplasms) : Repression of GADD153/CHOP by NF-kappaB : a possible cellular defense against endoplasmic reticulum stress induced cell death
Chae et al., Int Immunopharmacol 2001 : The NF-kappaB inhibitor also transiently activates p38 MAPK but SB203580, a specific p38 MAPK inhibitor, does not have any regulatory effect on PDTC induced cell death, suggesting that the cell death is mediated by JNK not by p38 MAPK
Funakoshi et al., Int Immunopharmacol 2001 : We used two specific inhibitors, SB203580 which selectively inhibits p38 MAP kinase and LY294002 which inhibits PI3-kinase, respectively, to explore the involvement of these kinases in the IL-1 induced NF-kappa B activation, using a human glioblastoma cell line, T98G ... In this context, pretreatment of LY294002, but not SB203580, inhibited IL-1 induced NF-kappa B activation significantly ... These observations were prominent particularly in the TRAF6 transfection system, in which overexpression of wild type TRAF6 augmented the IL-1 mediated NF-kappa B and AP-1 activation, while dominant negative TRAF6 construct ( delta TRAF6 ) suppressed these activation ... These observations were prominent particularly in the TRAF6 transfection system, in which overexpression of wild type TRAF6 augmented the IL-1 mediated NF-kappa B and AP-1 activation, while dominant negative TRAF6 construct ( delta TRAF6 ) suppressed these activation ... Namely, LY294002 inhibited TRAF6 mediated IL-1 induced NF-kappa B and AP-1 activation markedly, while SB203580 inhibited TRAF6 induced AP-1 activation but not NF-kappa B activation ... Namely, LY294002 inhibited TRAF6 mediated IL-1 induced NF-kappa B and AP-1 activation markedly, while SB203580 inhibited TRAF6 induced AP-1 activation but not NF-kappa B activation ... Above results indicated that both PI3-kinase and p38 MAP kinase are differentially involved in IL-1 induced NF-kappa B and AP-1 activation ... Above results indicated that both PI3-kinase and p38 MAP kinase are differentially involved in IL-1 induced NF-kappa B and AP-1 activation
Misra et al., Arch Biochem Biophys 2001 (Calcium Signaling...) : We conclude that alpha2M* induced cPLA2 synthesis is controlled by [ Ca2+ ] i levels, tyrosine kinase activity, the p21ras dependent MAPK and PI 3-kinase downstream signaling pathways, and regulation of NFkappaB ... We conclude that alpha2M* induced cPLA2 synthesis is controlled by [ Ca2+ ] i levels, tyrosine kinase activity, the p21ras dependent MAPK and PI 3-kinase downstream signaling pathways, and regulation of NFkappaB ... We conclude that alpha2M* induced cPLA2 synthesis is controlled by [ Ca2+ ] i levels, tyrosine kinase activity, the p21ras dependent MAPK and PI 3-kinase downstream signaling pathways, and regulation of NFkappaB
Kamthong et al., Biochem J 2001 : We have recently reported that interleukin-1alpha (IL-1alpha) can induce human macrophage colony stimulating factor ( M-CSF ) expression through nuclear factor kappaB (NF-kappaB) activation, and treatment of human pancreatic MIA PaCa-2 cancer cells with forskolin or cAMP attenuated the NF-kappaB activation as well as M-CSF expression ... We have recently reported that interleukin-1alpha (IL-1alpha) can induce human macrophage colony stimulating factor ( M-CSF ) expression through nuclear factor kappaB (NF-kappaB) activation, and treatment of human pancreatic MIA PaCa-2 cancer cells with forskolin or cAMP attenuated the NF-kappaB activation as well as M-CSF expression
Davis et al., Free Radic Biol Med 2001 : Soy isoflavone supplementation in healthy men prevents NF-kappa B activation by TNF-alpha in blood lymphocytes ... In this study, we have demonstrated that genistein, but not daidzein, inhibits TNF-alpha induced NF-kappa B activation in cultured human lymphocytes ... Additionally, we investigated the in vivo effect of soy isoflavone supplementation on NF-kappa B activation induced by TNF-alpha in vitro in peripheral blood lymphocytes of six healthy men ... We show that healthy male subjects receiving 50 mg isoflavone mixture ( Novasoy ) twice daily for 3 weeks are protected from TNF-alpha induced NF-kappa B activation
Bone et al., Int Immunol 2001 : Nuclear NF-kappaB is induced in B cells by engagement of either the BCR or CD40 or by stimulation with lipopolysaccharide (LPS) ... Nuclear NF-kappaB is induced in B cells by engagement of either the BCR or CD40 or by stimulation with lipopolysaccharide (LPS) ... Nuclear NF-kappaB is induced in B cells by engagement of either the BCR or CD40 or by stimulation with lipopolysaccharide (LPS) ... In this report we address the role of phosphoinositide 3'-kinase ( PI 3-kinase ) in BCR- and LPS induced NF-kappaB activation using populations of primary murine resting B cells ... Using the specific pharmacological inhibitors of PI 3-kinase, Wortmannin and LY294002, we demonstrate that PI 3-kinase activity is vital for BCR induced NF-kappaB DNA binding activity
Ruiz-Ortega et al., Nephrol Dial Transplant 2001 : In cultured VSMCs from wild-type mice, Ang II increased NF-kappaB activity that was partially inhibited by AT1 ( losartan ) and AT2 ( PD123319 ) antagonists ... In VSMC from AT1 knockout mice, Ang II also activated NF-kappaB
Grewal et al., J Biol Chem 2001 : This transactivation resulted in the initiation of multiple cellular signals ( phosphorylation of the EGFRs and ERK and activation of cAMP-responsive element binding protein ( CREB ), NF-kappaB , and E2F ), as well as subsequent rapid down-regulation of cell-surface EGFRs and internalization and desensitization of the EGFRs without change in the total cellular complement of EGFRs
Chung et al., Biochem Biophys Res Commun 2001 : Hepatitis C virus core protein potentiates TNF-alpha induced NF-kappaB activation through TRAF2-IKKbeta dependent pathway ... Previous work has implicated that the core protein of hepatitis C virus ( HCV ) may play a modulatory effect on NF-kappaB activation induced by TNF-alpha ... Here we show that overexpression of HCV core protein potentiates NF-kappaB activation induced by TNF-alpha ... Expression of dominant negative form of TRAF2 inhibits the synergistic effects of HCV core protein on NF-kappaB activation, suggesting that HCV core protein potentiates NF-kappaB activation through TRAF2 ... Expression of dominant negative form of TRAF2 inhibits the synergistic effects of HCV core protein on NF-kappaB activation, suggesting that HCV core protein potentiates NF-kappaB activation through TRAF2 ... Moreover, we demonstrate that HCV core protein potentiates TRAF2 mediated NF-kappaB activation via IKKbeta ... Moreover, we demonstrate that HCV core protein potentiates TRAF2 mediated NF-kappaB activation via IKKbeta ... In addition, HCV core protein associates with TNF-R1-TRADD-TRAF2 signaling complex, resulting in synergistically activation of NF-kappaB induced by TNF-alpha
Spiegelman et al., J Biol Chem 2001 : Induction of beta-transducin repeat containing protein by JNK signaling and its role in the activation of NF-kappaB
Bond et al., Cardiovasc Res 2001 : IL-1alpha potently activated NF-kappa B in VSMCs and acted synergistically with growth factors to upregulate expression of MMP-1, -3 and -9
Suk et al., Immunol Lett 2001 : The regulation of microglial activation by IFNgamma and PGE ( 2 ) was accompanied by differential modulation of LPS induced NF-kB activation ... While IFNgamma enhanced LPS induced NF-kB activation, PGE ( 2 ) suppressed its activation ... While IFNgamma enhanced LPS induced NF-kB activation , PGE ( 2 ) suppressed its activation
Müller et al., Immunology 2001 (Translocation, Genetic) : An approximately 70 % decrease of lipopeptide induced NFkappaB translocation and an about 50 % reduction of nitric oxide ( NO ) release was observed in the presence of anti-CD14
Chatterjee et al., Kidney Int 2001 (Acute Kidney Injury...) : Cal I-1 also reduced histologic evidence of I/R mediated renal damage and caused a substantial reduction in the expression of iNOS and COX-2, both of which involve activation of nuclear factor-kappa B (NF-kappa B) ... Cal I-1 also reduced histologic evidence of I/R mediated renal damage and caused a substantial reduction in the expression of iNOS and COX-2 , both of which involve activation of nuclear factor-kappa B (NF-kappa B)
Purcell et al., Proc Natl Acad Sci U S A 2001 (Cardiomegaly) : The activation of NF-kappaB was inhibited by expression of a `` supersuppressor '' IkappaBalpha mutant that is resistant to stimulation induced degradation and a dominant negative IkappaB kinase ( IKKbeta ) mutant that can no longer be activated by phosphorylation
Murono et al., Proc Natl Acad Sci U S A 2001 (Nasopharyngeal Neoplasms...) : Coexpression of IkappaBalpha ( S32A/S36A ), which is not phosphorylated and prevents NF-kappaB activation, with LMP1 showed that NF-kappaB is essential for induction of COX-2 by LMP1 ... We also demonstrate that NF-kappaB is involved in LMP1 induced cox-2 promoter activity with the use of reporter assays
Varela et al., J Cell Physiol 2001 (Adenocarcinoma...) : TNFalpha induces NFkappaB/p50 in association with the growth and morphogenesis of normal and transformed rat mammary epithelial cells ... However, in transformed MEC, NFkappaB binding was initially undetectable but then increased in response to TNFalpha
Allport et al., Mol Hum Reprod 2001 : In cells obtained before labour, in which NF-kappaB activity is low, increasing the concentration of PR represses NF-kappaB dependent transcription, while stimulation with IL-1beta both increases NF-kappaB activity and represses PR activity
Li et al., Pediatr Res 2001 (Meconium Aspiration Syndrome) : Meconium induces expression of inducible NO synthase and activation of NF-kappaB in rat alveolar macrophages
Kraft et al., Int Arch Allergy Immunol 2001 (Hypersensitivity, Immediate) : Inhibitors of NF-kappa B activation such as N-acetylcysteine or N-tosyl-L-phenylalanine chloromethyl ketone can suppress Fc epsilon RI-induced TNF-alpha and MCP-1 release ... Inhibitors of NF-kappa B activation such as N-acetylcysteine or N-tosyl-L-phenylalanine chloromethyl ketone can suppress Fc epsilon RI-induced TNF-alpha and MCP-1 release
Hugot et al., Nature 2001 (Colitis, Ulcerative...) : NOD2 activates nuclear factor NF-kB ; this activating function is regulated by the carboxy-terminal leucine-rich repeat domain, which has an inhibitory role and also acts as an intracellular receptor for components of microbial pathogens
Ogura et al., Nature 2001 (Crohn Disease...) : Wild-type NOD2 activates nuclear factor NF-kappaB , making it responsive to bacterial lipopolysaccharides ; however, this induction was deficient in mutant NOD2
Ehret et al., Eur J Immunol 2001 : HIV Tat enhances activation of NF-kappaB and consequently, activates the AP-1 ( IL-2/d ) site
O'Suilleabhain et al., Shock 2001 (Burns) : Our findings revealed that changes in mitogen stimulated T-cell AP-1 and NFkappaB factor activation correlated directly with defective mitogen induced IL-2 mRNA expression
Brasier et al., J Biol Chem 2001 : Down-regulation of BCL-3 induction results in prolonged, enhanced NF-kappa B1 binding and increased NF-kappa B-dependent transcription
McAllister-Lucas et al., J Biol Chem 2001 (Lymphoma, B-Cell, Marginal Zone) : Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10 mediated NF-kappaB induction ... Bimp1 mediated NF-kappaB activation requires Bcl10 and IkappaB kinases, indicating that Bimp1 acts upstream of these mediators
Ludwig et al., Cancer Res 2001 (Carcinoma, Medullary...) : RET induced NF-kappaB and IKKbeta activity requires Ras function but does neither involve the classical mitogen activated protein kinase kinase/extracellular signal regulated kinase nor the phosphoinositide 3-kinase/Akt pathways ... RET induced NF-kappaB and IKKbeta activity requires Ras function but does neither involve the classical mitogen activated protein kinase kinase/extracellular signal regulated kinase nor the phosphoinositide 3-kinase/Akt pathways ... In contrast, RET induced NF-kappaB activity is dependent on Raf and MEKK1 ... In contrast, RET induced NF-kappaB activity is dependent on Raf and MEKK1
Murley et al., Free Radic Biol Med 2001 : Neither catalase nor pyruvate when added to the culture medium to minimize hydrogen peroxide production had an effect on NFkappaB activation by SH
Anthonsen et al., J Biol Chem 2001 : Functional coupling between secretory and cytosolic phospholipase A2 modulates tumor necrosis factor-alpha- and interleukin-1beta induced NF-kappa B activation ... Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta are potent activators of the transcription factor NF-kappaB , induced during inflammatory conditions ... Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta are potent activators of the transcription factor NF-kappaB , induced during inflammatory conditions ... Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta are potent activators of the transcription factor NF-kappaB , induced during inflammatory conditions ... We have previously shown that both secretory and cytosolic phospholipase A ( 2 ) ( PLA(2) ) are involved in TNF-alpha- and IL-1beta induced NF-kappaB activation ... We show that in addition to inhibitors of secretory and cytosolic PLA(2)s, 5-lipoxygenase inhibitors attenuate TNF-alpha- and IL-1beta stimulated NF-kappaB activation ... We show that in addition to inhibitors of secretory and cytosolic PLA(2)s, 5-lipoxygenase inhibitors attenuate TNF-alpha- and IL-1beta stimulated NF-kappaB activation ... Our results therefore suggest the existence of a functional link between secretory and cytosolic PLA(2) in cytokine activated keratinocytes, providing a molecular explanation for the participation of both secretory and cytosolic PLA(2) in arachidonic acid signaling and NF-kappaB activation in response to proinflammatory cytokines
Van Huffel et al., J Biol Chem 2001 : NF-kappaB activation induced by TNF and interleukin-1 is inhibited by overexpression of ABIN-2 ... NF-kappaB activation induced by TNF and interleukin-1 is inhibited by overexpression of ABIN-2 ... NF-kappaB activation induced by TNF and interleukin-1 is inhibited by overexpression of ABIN-2 ... The latter also inhibits NF-kappaB activation induced by overexpression of receptor interacting protein or TNF receptor associated factor 2 ... In contrast, NF-kappaB activation by overexpression of IKKbeta or direct activators of the IKK complex, such as Tax, can not be inhibited by ABIN-2 ... In contrast, NF-kappaB activation by overexpression of IKKbeta or direct activators of the IKK complex, such as Tax, can not be inhibited by ABIN-2 ... In contrast, NF-kappaB activation by overexpression of IKKbeta or direct activators of the IKK complex , such as Tax, can not be inhibited by ABIN-2
Abu-Amer et al., J Clin Invest 2001 : IL-4 abrogates osteoclastogenesis through STAT6 dependent inhibition of NF-kappaB ... It is shown here that IL-4 reduces NF-kappaB nuclear translocation by inhibiting IkappaB phosphorylation, thus markedly inhibiting NF-kappaB DNA binding activity and blocking osteoclastogenesis entirely ... Residual translocation of NF-kappaB in the presence of IL-4 , however, suggests that nuclear mechanisms must primarily account for inhibition of NF-kappaB DNA binding and blockade of osteoclastogenesis ... To address this issue, this study examined whether IL-4 induced STAT6 transcription factor blocks NF-kappaB transactivation ... The results show that excess unlabeled consensus sequence STAT6 , but not its mutated form, inhibits NF-kappaB binding
Li et al., J Immunol 2001 (Inflammation...) : Intriguingly, NF-kappaB activation by necrotic cells was dependent on Toll-like receptor 2 , a signaling pathway that induces inflammation in response to microbial agents
Yoshida et al., J Immunol 2001 : Inhibition of NADPH oxidase suppressed NF-kappaB binding by nuclear extracts and decreased production of MMP-2 and 9 in AMs from SP-D ( -/- ) mice
Johnson et al., J Virol 2001 : The cellular kinases Akt and p70S6K and the transcription factor NF-kappaB were activated in a PI3-K dependent manner at similar times following HCMV infection
Ros et al., Hepatology 2001 (Carcinoma, Hepatocellular...) : Induction of Mdr1b expression by tumor necrosis factor-alpha in rat liver cells is independent of p53 but requires NF-kappaB signaling ... Induction of Mdr1b expression by tumor necrosis factor-alpha in rat liver cells is independent of p53 but requires NF-kappaB signaling ... We show a critical role for NF-kappaB in activation of Mdr1b gene expression both in primary rat hepatocytes and in rat hepatoma H-4-II-E cells ... Because p53 is up-regulated by TNF-alpha in an NF-kappaB dependent manner and the Mdr1b promoter contains a p53 binding site, we used liver cells expressing a dominant negative p53 to show that TNF-alpha up-regulation of Mdr1b is independent of functional p53 ... Because p53 is up-regulated by TNF-alpha in an NF-kappaB dependent manner and the Mdr1b promoter contains a p53 binding site, we used liver cells expressing a dominant negative p53 to show that TNF-alpha up-regulation of Mdr1b is independent of functional p53 ... In conclusion our results show that activation of the rat Mdr1b gene by TNF-alpha is a result of NF-kappaB signaling and independent of p53 ... In conclusion our results show that activation of the rat Mdr1b gene by TNF-alpha is a result of NF-kappaB signaling and independent of p53
Renard et al., Antioxid Redox Signal 2001 : Using various antioxidants, including pyrrolidine dithiocarbamate and curcumin, we were unable to show a direct relationship between this IL-1 induced glutathione oxidation and NF-kappaB activation ... Of the five antioxidants tested, only curcumin was able to inhibit IkappaBalpha degradation upstream and, hence, NF-kappaB DNA binding activity and NF-kappaB dependent expression of IL-6 downstream
Naik et al., J Pediatr Gastroenterol Nutr 2001 (Inflammation...) : In Caco-2 cells, significant NFkappaB activation in response to LPS occurred only in the presence of TLR4 introduced by complementary deoxyribonucleic acid transfection ... In Caco-2 cells, significant NFkappaB activation in response to LPS occurred only in the presence of TLR4 introduced by complementary deoxyribonucleic acid transfection
Zhang et al., J Clin Endocrinol Metab 2001 : TNF alpha increased the messenger ribonucleic acid levels of ICE, TNF alpha, IL-1 beta, bcl-2, and NF kappa B in preadipocytes and adipocytes ( P < 0.01 )
Hattori et al., Eur Cytokine Netw 2001 (Melanoma) : Since TNF induced NF-kappa B activation, cell cycle arrest, RB dephosphorylation, and E2F downregulation are indistinguishable in both cell lines, none of these factors is likely to be involved in the TNF induced anti-apoptotic mechanism in A375-6 cells
Whan Han et al., Br J Pharmacol 2001 : Ergolide inhibited nuclear factor-kappaB (NF-kappaB) activation, a transcription factor necessary for iNOS and COX-2 expression in response to LPS/IFN-gamma
Chen et al., Biochem Biophys Res Commun 2001 : In human vascular endothelial cells ( ECV304 cells ), troglitazone inhibited TNFalpha induced ICAM-1 gene expression by suppressing NF-kappaB/DNA binding activity, NF-kappaB transcriptional responses, c-Fos mRNA and protein levels via a ligand dependent, PPARgamma activated manner ... In human vascular endothelial cells ( ECV304 cells ), troglitazone inhibited TNFalpha induced ICAM-1 gene expression by suppressing NF-kappaB/DNA binding activity, NF-kappaB transcriptional responses , c-Fos mRNA and protein levels via a ligand dependent, PPARgamma activated manner
Sanlioglu et al., J Biol Chem 2001 : Lipopolysaccharide induces Rac1 dependent reactive oxygen species formation and coordinates tumor necrosis factor-alpha secretion through IKK regulation of NF-kappa B ... The present study demonstrates for the first time that the activation of Rac1 ( a GTP binding protein ), and the subsequent production of ROS, constitutes a major pathway involved in NFkappaB mediated tumor necrosis factor-alpha (TNFalpha) secretion following LPS challenge in macrophages ... Expression of a dominant negative mutant of Rac1 ( N17Rac1 ) reduced Rac1 activation, ROS formation, NFkappaB activation, and TNFalpha secretion following LPS stimulation ... IKKalpha and IKKbeta were both required downstream modulators of LPS activated Rac1, since the expression of either of the IKK dominant mutants ( IKKalphaKM or IKKbetaKA ) drastically reduced NFkappaB dependent TNFalpha secretion
Ivanov et al., Oncogene 2001 (Melanoma) : Conversely, activation of IKK attenuates the sensitization of melanoma by TRAF2DeltaN, indicating that p38 mediated suppression of NF-kappaB activity is among TRAF2DeltaN effects ... Our finding identifies p38, TNFalpha and NF-kappaB among key players that efficiently sensitizes melanoma cells to UV-, ribotoxic ( anisomycin ) and radiomimetic chemicals induced programmed cell death in response to aberrant TRAF2 signaling
Jiang et al., Oncogene 2001 (Adenocarcinoma...) : To evaluate the role of the NF-kappaB signaling pathway in oncogenic transformation, we expressed IkappaBbeta , a specific inhibitor of NF-kappaB , in two human lung adenocarcinoma cell lines, A549 and H441 ... Expression of IkappaBbeta significantly reduced NF-kappaB activation induced by cotransfection with p65/RelA or TNF-alpha and abrogated the basal NF-kappaB activity in A549 cells
Balliet et al., DNA Cell Biol 2001 : Comparison of putative transcription factor binding sites found in sequences of this gene family provides evidence that p53 is involved in regulating the expression of MyD118 and that NF-kappaB may play a role in differential expression of MyD118 and Gadd45 ( Gadd45alpha )
Jones et al., J Leukoc Biol 2001 (MAP Kinase Signaling System) : Stimulation of RAW 264.7 macrophages by LPS , LAM, STF, and PIM rapidly activated nuclear factor (NF)-kappaB , activator protein-1 (AP-1), and mitogen activated protein ( MAP ) kinases ... Stimulation of RAW 264.7 macrophages by LPS, LAM, STF, and PIM rapidly activated nuclear factor (NF)-kappaB , activator protein-1 (AP-1), and mitogen activated protein ( MAP ) kinases
Yang et al., Cancer Res 2001 (Melanoma) : Constitutive IKK activity associated with increased IkappaBalpha phosphorylation and degradation contribute to the high level of endogenous nuclear factor-kappaB (NF-kappaB) activation in Hs294T melanoma cells as compared with RPE cells ( R. L. Shattuck-Brandt and A. Richmond, Cancer Res., 57 : 3032-3039, 1997 ; M. N. Devalaraja et al., Cancer Res., 59 : 1372-1377, 1999 )
Galindo et al., Arthritis Rheum 2001 (Scleroderma, Systemic) : Increased NF-kappaB or AP-1 activation was not responsible for the constitutive overexpression of MCP-1 by SSc fibroblasts
Knecht et al., Oncology 2001 (Cell Transformation, Neoplastic...) : TRAF2 mediated NF-kappaB activation, AP-1 induction and JAK3/STAT activation may result in sustained proliferation leading to lymphoma ... TRAF2 mediated NF-kappaB activation, AP-1 induction and JAK3/STAT activation may result in sustained proliferation leading to lymphoma ... TRAF2 mediated NF-kappaB activation, AP-1 induction and JAK3/STAT activation may result in sustained proliferation leading to lymphoma ... TRAF2 mediated NF-kappaB activation , AP-1 induction and JAK3/STAT activation may result in sustained proliferation leading to lymphoma
Wolf et al., Clin Cancer Res 2001 (Carcinoma, Squamous Cell...) : In this study, we examined the role of IL-1alpha in the activation of NFkappaB and AP-1, the expression of proangiogenic cytokine IL-8, and in the survival and proliferation of HNSCC cell lines ... In this study, we examined the role of IL-1alpha in the activation of NFkappaB and AP-1, the expression of proangiogenic cytokine IL-8 , and in the survival and proliferation of HNSCC cell lines ... IL-1alpha was found to induce coexpression of IL-8 through the activation of NFkappaB and AP-1, in that mutation of the NFkappaB site within the IL-8 promoter abolished autocrine- and recombinant IL-1alpha induced IL-8 reporter gene activity, whereas mutation in AP-1 partially decreased IL-8 reporter gene activity in UM-SCC-9 cells
Heiss et al., J Biol Chem 2001 : SFN selectively reduced DNA binding of NF-kappa B without interfering with LPS induced degradation of the inhibitor of NF-kappa B nor with nuclear translocation of NF-kappa B
Mathiasen et al., Int J Cancer 2001 : Furthermore, 1,25 ( OH ) ( 2 ) D ( 3 ) enhanced TNF induced NF-kappaB activation in T47D cells suggesting that it potentiates TNF signaling in general
Huang et al., Mol Cell Biol 2001 : The increased synthesis of IkappaBalpha leads to postinduction repression of nuclear NF-kappaB activity
Hong et al., FEBS Lett 2001 : CSN3 interacts with IKKgamma and inhibits TNF- but not IL-1 induced NF-kappaB activation ... Activation of NF-kappaB requires the activity of IKK , a kinase complex that contains two catalytic subunits, IKKalpha and IKKbeta, and a regulatory subunit IKKgamma ... Overexpression of CSN3 inhibits NF-kappaB activation triggered by tumor necrosis factor (TNF), but not interleukin-1 (IL-1) ... Moreover, overexpression of CSN3 also inhibits NF-kappaB activation triggered by proteins involved in TNF signaling, including TNF-R1, TRAF2, RIP, and NIK, but not by TRAF6, a protein involved in IL-1 signaling
Osawa et al., J Immunol 2001 : Although Akt has been reported to activate NF-kappaB, DMS and LY 294002 failed to prevent TNF-alpha induced NF-kappaB activation , suggesting that the antiapoptotic effects of SphK and Akt are independent of NF-kappaB
Wang et al., J Immunol 2001 : Human Notch-1 inhibits NF-kappa B activity in the nucleus through a direct interaction involving a novel domain ... We found that Notch ( IC ) localizes to the nucleus and that a region in the N-terminal portion of Notch ( IC ), not the six ankyrin repeats, is responsible for the inhibitory effects of Notch on NF-kappaB directed gene expression and NF-kappaB DNA binding activity
Smith et al., J Immunol 2001 : IL-8 production in human lung fibroblasts and epithelial cells activated by the Pseudomonas autoinducer N-3-oxododecanoyl homoserine lactone is transcriptionally regulated by NF-kappa B and activator protein-2 ... It was found that the DNA region between nucleotides -1481 and -546 and the transcription factor NF-kappaB were essential for the maximal induction of IL-8 by 3-O-C12-HSL
Schwarz et al., J Immunol 2001 (Inflammation) : Kaposi 's sarcoma associated herpesvirus G protein coupled receptor constitutively activates NF-kappa B and induces proinflammatory cytokine and chemokine production via a C-terminal signaling determinant
Hall et al., Circ Res 2001 : Deletion of the redox-sensitive domain of Ref-1 significantly inhibited TNF induced NF-kappaB activation ... Moreover, it seems that Ref-1 may act as a critical cofactor, mediating the TNF induced NF-kappaB response in the vascular endothelium
Köhler et al., Vet Dermatol 2001 : TNF-alpha dependent NF-kappa B activation in cultured canine keratinocytes is partly mediated by reactive oxygen species
Maaser et al., Clin Exp Immunol 2001 : Inhibition of endothelial NF-kappaB activation using the proteosome inhibitors MG-132 and BAY 11-7082 resulted in a significant decrease of ICAM-1 expression, indicating an important role for NF-kappaB in this response
Zhang et al., Cell Death Differ 2001 (MAP Kinase Signaling System) : Neither U0126 nor LY294002 pretreatment affected TNF induced activation of NF-kappaB , suggesting that the MAP kinase or PI3-kinase/Akt mediated anti-apoptotic effect induced by TNF was not relevant to NF-kappaB activation
Matsumoto et al., J Biol Chem 2001 : Regulation of receptor activator of NF-kappaB ligand induced tartrate-resistant acid phosphatase gene expression by PU.1 interacting protein/interferon regulatory factor-4
Blondeau et al., J Neurosci 2001 (Brain Ischemia...) : Preconditioning probably inhibits the activation of NFkappaB by interfering with a pathway that leads to the direct transcriptional activation of IkappaBalpha by NFkappaB itself
Bian et al., Exp Eye Res 2001 (MAP Kinase Signaling System) : Furthermore, co-administration of U0126 and SB202190 did not affect the induced degradation of IkappaB-alpha and NF-kappaB nuclear translocation, indicating that NF-kappaB is activated by IL-1beta and TNF-alpha independently of activation of MEK/MAPK and p38 pathways in hRPE cells ... Furthermore, co-administration of U0126 and SB202190 did not affect the induced degradation of IkappaB-alpha and NF-kappaB nuclear translocation, indicating that NF-kappaB is activated by IL-1beta and TNF-alpha independently of activation of MEK/MAPK and p38 pathways in hRPE cells
Wang et al., Br J Pharmacol 2001 : Pretreatment of rats with pyrrolidine dithiocarbamate ( PDTC ) suppresses LPS-, but not PAF- , induced NF-kappaB and C/EBP binding activity, and significantly increases the C/EBP-delta subunit in LPS- or PAF induced C/EBP complex ... Pretreatment of rats with pyrrolidine dithiocarbamate ( PDTC ) suppresses LPS- , but not PAF-, induced NF-kappaB and C/EBP binding activity, and significantly increases the C/EBP-delta subunit in LPS- or PAF induced C/EBP complex
Yang et al., Nat Immunol 2001 : The essential role of MEKK3 in TNF induced NF-kappaB activation ... The essential role of MEKK3 in TNF induced NF-kappaB activation ... Using mitogen activated protein kinase kinase kinase 3 ( MEKK3 ) -deficient fibroblast cells, we found that MEKK3 plays a critical role in TNF induced NF-kappaB activation ... Using mitogen activated protein kinase kinase kinase 3 ( MEKK3 ) -deficient fibroblast cells, we found that MEKK3 plays a critical role in TNF induced NF-kappaB activation ... The kinase activity of MEKK3 is pivotal to its function and, therefore, MEKK3 links RIP and IKK in TNF induced NF-kappaB activation
Ye et al., J Neuroimmunol 2001 : The purpose of this study was to determine if binding of nuclear factor kappaB (NFkappaB) to the IL-6 promoter is responsible for the age related increase in brain IL-6
Stramer et al., Invest Ophthalmol Vis Sci 2001 : Because IkappaBalpha expression is induced by nuclear factor (NF)-kappaB , this finding suggests that rates of constitutive NF-kappaB activation, and thus IkappaBalpha degradation, are elevated in activated stromal cells
Factor et al., Hepatology 2001 (Liver Neoplasms, Experimental) : Roles of Akt/PKB and IKK complex in constitutive induction of NF-kappaB in hepatocellular carcinomas of transforming growth factor alpha/c-myc transgenic mice ... Roles of Akt/PKB and IKK complex in constitutive induction of NF-kappaB in hepatocellular carcinomas of transforming growth factor alpha/c-myc transgenic mice ... Roles of Akt/PKB and IKK complex in constitutive induction of NF-kappaB in hepatocellular carcinomas of transforming growth factor alpha/c-myc transgenic mice ... Lastly, activation of the Akt/protein kinase B (PKB), which has recently been implicated in NF-kappaB activation by PDGF, TNF-alpha , and Ras, was also observed
Drumm et al., Eur J Med Res 2001 (Albuminuria) : We investigated albumin induced NF-kappaB activation and the potential contribution of tyrosine kinase and protein kinase C ... Therefore we exposed proximal tubule derived human ( IHKE-1 ), opossum ( OK ) and porcine ( LLC-PK1 ) cell lines to serum albumin at concentrations of 10 - 500 mg/l. DNA binding activity of NF-kappaB increased concentration-dependently in the presence of albumin ... The albumin induced increase in NF-kappaB DNA binding activity was inhibited by herbimycin A ( tyrosine kinase inhibitor ) and BIM ( protein kinase C inhibitor )
Yoshimura et al., Eur J Immunol 2001 : As PSI is not a specific inhibitor of NFkappaB, we reproduced this finding using a very specific approach, namely adenoviral gene transfer of IkappaBalpha , the naturally occurring inhibitor of NFkappaB
Shi et al., J Biol Chem 2001 : Placing PYK2 upstream of both PI3K and Akt activation, PYK2 activated Akt through a PI3K dependent pathway, and either a dominant negative form of Akt or the PI3K inhibitor LY294002 blocked PYK2 stimulated NF-kappa B-dependent transcription
Adib-Conquy et al., J Leukoc Biol 2001 (Wounds and Injuries) : In vitro lipopolysaccharide (LPS) stimulation of PBMCs induced NF-kappa B translocation ... The long-term low-basal and LPS induced nuclear translocation of NF-kappa B recalled long-term immunoparalysis observed in patients with severe inflammatory stress such as trauma
Takahashi et al., J Virol 2001 : Furthermore, enhanced HIV-1 transcription in gp34 stimulated ACH-2/OX40 cells was dependent on the kappa B site of the HIV-1 long terminal repeat, and the OX40-gp34 interaction activated NF-kappa B consisting of p50 and p65 subunits
Kubota et al., J Mol Cell Cardiol 2001 (Heart Failure) : Furthermore, TNF- alpha overexpression activated NF- kappa B , a mediator of anti-apoptotic pathways, in the myocardium
Kanai et al., Gastroenterology 2001 : TGF-alpha also enhanced the expression of Bcl-2 family proteins in an NF-kappaB dependent manner
Shuto et al., Proc Natl Acad Sci U S A 2001 (MAP Kinase Signaling System) : The NF-kappa B translocation dependent pathway involves activation of NF-kappa B inducing kinase (NIK) -- IKK alpha/beta complex leading to I kappa B alpha phosphorylation and degradation, whereas the NF-kappa B translocation independent pathway involves activation of MKK3/6 -- p38 mitogen activated protein ( MAP ) kinase pathway ... The NF-kappa B translocation dependent pathway involves activation of NF-kappa B inducing kinase (NIK) -- IKK alpha/beta complex leading to I kappa B alpha phosphorylation and degradation, whereas the NF-kappa B translocation independent pathway involves activation of MKK3/6 -- p38 mitogen activated protein ( MAP ) kinase pathway ... Furthermore, we show that toll-like receptor 2 (TLR2) is required for NTHi induced NF-kappa B activation
Ganster et al., Proc Natl Acad Sci U S A 2001 : Complex regulation of human inducible nitric oxide synthase gene transcription by Stat 1 and NF-kappa B
Oya et al., Oncogene 2001 (Carcinoma, Renal Cell...) : Forced expression of antisense cDNA of IkappaBalpha , a specific inhibitor of NF-kappaB , in TRAIL-sensitive cell lines with a low NF-kappaB activity result in constitutive activation of NF-kappaB and resistance to TRAIL induced apoptosis
Natarajan et al., J Interferon Cytokine Res 2001 (Crohn Disease) : Redox imbalance in Crohn 's disease intestinal smooth muscle cells causes NF-kappaB mediated spontaneous interleukin-8 secretion
Delgado et al., J Immunol 2001 (Acute Disease...) : The VIP/PACAP inhibition of both chemokine production and of NF-kappaB binding and transactivating activity is mediated through the specific VIP receptor VPAC1, and involves both cAMP dependent and -independent intracellular pathways ... The VIP/PACAP inhibition of both chemokine production and of NF-kappaB binding and transactivating activity is mediated through the specific VIP receptor VPAC1, and involves both cAMP dependent and -independent intracellular pathways
Bulut et al., J Immunol 2001 : Moreover, we show that Toll interacting protein (Tollip) coimmunoprecipitates with TLR2 and TLR4 using HEK 293 cells, and overexpression of Tollip inhibits NF-kappaB activation in response to TLR2 and TLR4 signaling ... Moreover, we show that Toll interacting protein (Tollip) coimmunoprecipitates with TLR2 and TLR4 using HEK 293 cells, and overexpression of Tollip inhibits NF-kappaB activation in response to TLR2 and TLR4 signaling ... Moreover, we show that Toll interacting protein (Tollip) coimmunoprecipitates with TLR2 and TLR4 using HEK 293 cells, and overexpression of Tollip inhibits NF-kappaB activation in response to TLR2 and TLR4 signaling
Khan et al., Immunol Res 2001 (Agammaglobulinemia) : Furthermore, BTK dependent activation of NF-kappaB is essential for reprogramming the expression of genes that control B cell survival and proliferation
Kim et al., Scand J Gastroenterol 2001 (Adenocarcinoma...) : We studied the role of NF-kappaB on expression of IL-8 and COX-2 in H. pylori stimulated AGS gastric epithelial cells by using antisense oligonucleotide ( AS ODN ) for NF-kappaB subunit p50 and an antioxidant, glutathione ( GSH ) as well as a NF-kappaB inhibitor, pyrrolidine dithiocarbamate ( PDTC ) ... We studied the role of NF-kappaB on expression of IL-8 and COX-2 in H. pylori stimulated AGS gastric epithelial cells by using antisense oligonucleotide ( AS ODN ) for NF-kappaB subunit p50 and an antioxidant, glutathione ( GSH ) as well as a NF-kappaB inhibitor, pyrrolidine dithiocarbamate ( PDTC ) ... NF-kappaB may play a novel role in expression of IL-8 and COX-2 in H. pylori induced gastric inflammation ... NF-kappaB may play a novel role in expression of IL-8 and COX-2 in H. pylori induced gastric inflammation
Crane et al., Cell Immunol 2001 (Calcium Signaling) : IL-1beta stimulated GM-CSF mRNA expression and production was strongly dependent on NF-kappaB ... The results show that IFN-gamma down-regulation acts either directly on NF-kappaB or its activation or by blockade of a pathway upstream of NF-kappaB
Yang et al., Am J Physiol Gastrointest Liver Physiol 2001 (Fatty Liver) : Fatty liver vulnerability to endotoxin induced damage despite NF-kappaB induction and inhibited caspase 3 activation
Soriani et al., Infect Immun 2001 : Myc activation was also associated with an increase of IkappaBalpha turnover, suggesting that elevated Myc expression may be dependent on NF-kappaB
Craig et al., J Biol Chem 2001 : Together, the inhibitors completely blocked CT-1 dependent NF-kappa B activation and cytoprotection
Sakai et al., J Bone Miner Res 2001 : Thus, we investigated whether TNF-alpha activates a transcription factor nuclear factor kappaB (NF-kappaB) in human chondrocyte-like cells ( HCS-2/8 ) and induces the expression of genes involved in the degradation of cartilage matrix ... Thus, it is shown that the activation of p65/p50 NF-kappaB by TNF-alpha plays a cardinal role in inducing the expression of MMP-1, MMP-3, ICAM-1, and COX-2 genes, which are involved in matrix degradation and inflammatory reaction in chondrocytes, leading to chondrocytic chondrolysis
Hattori et al., Arterioscler Thromb Vasc Biol 2001 : NF-kappaB activation by LPS/IFN was inhibited by HNE in a dose dependent manner
Wang et al., Biochem Biophys Res Commun 2001 (Arteriosclerosis) : VEGF increased intracellular reactive oxygen species ( ROS ), NF-kappaB activation and IL-6 expression ... Blockade of the generation of intracellular ROS by antioxidants inhibited VEGF induced NF-kappaB activation, IL-6 expression, and cell migration indicating that generation of ROS was required for NF-kappaB activation and the chemotactic activity of VEGF
Weber et al., Chem Res Toxicol 2001 : Catalase fully inhibited peak TGHQ mediated TRE- and NF-kappaB binding activity
Vicente et al., Br J Pharmacol 2001 : Our results suggest that inhibition of NF-kappaB binding could mediate the effects of ZM 230,487 on the modulation of HO-1 and iNOS protein expression ... Our results suggest that inhibition of NF-kappaB binding could mediate the effects of ZM 230,487 on the modulation of HO-1 and iNOS protein expression
Izumi et al., J Clin Invest 2001 (Myocardial Ischemia...) : These results suggest that inhibition of GC-A alleviates ischemia/reperfusion injury through suppression of NF-kappaB mediated P-selectin induction
Jeon et al., Int Immunopharmacol 2001 : In conclusion, we demonstrate that angelan induces NF-kappa B/Rel activation through the CD14 and CR3 membrane receptor and p38 kinase that is critically involved in the signal transduction leading to NF-kappa B/Rel activation in murine macrophages
Lim et al., Free Radic Biol Med 2001 (Helicobacter Infections) : We studied the role of NF-kappaB on iNOS expression and apoptosis in H. pylori stimulated gastric epithelial AGS cells ... The cells treated with AS ODN had low levels of p50 and NF-kappaB and inhibited H. pylori induced cytotoxicity, apoptosis, iNOS expression, and nitrite production ... In conclusion, NF-kappaB plays a novel role in iNOS expression and apoptosis in H. pylori infected gastric epithelial cells
Lipsky et al., J Neurochem 2001 : Furthermore, nuclear extracts prepared from neurons treated with NMDA and the double stranded NF-kappaB oligonucleotide showed reduced DNA binding activity to the target sequence, supporting the idea that NF-kappaB may be involved in the transcriptional activation of the BDNF gene ... We also determined that BDNF itself induced an NF-kappaB DNA binding activity
Trauzold et al., Oncogene 2001 (Adenocarcinoma...) : We report here that the stimulation of the CD95- and TRAIL-resistant human pancreatic adenocarcinoma cell line PancTuI with an agonistic anti-CD95 antibody or TRAIL resulted in activation of protein kinase C and NF-kappaB ... We report here that the stimulation of the CD95- and TRAIL-resistant human pancreatic adenocarcinoma cell line PancTuI with an agonistic anti-CD95 antibody or TRAIL resulted in activation of protein kinase C and NF-kappaB ... Inhibition of protein kinase C by Gö6983 sensitized these cells to apoptotic challenges and strongly diminished activation of NF-kappaB by anti-CD95 and TRAIL ... Inhibition of protein kinase C by Gö6983 sensitized these cells to apoptotic challenges and strongly diminished activation of NF-kappaB by anti-CD95 and TRAIL
Dreikhausen et al., Eur J Immunol 2001 (MAP Kinase Signaling System) : UDP-monoglucosylation and concomitant inactivation of Rho A and of Rac-2 by Clostridium difficile toxin B also inhibited IL-1 induced activation of JNK and p38 MAPK , but additionally inhibited activation of the extracellular-regulated-kinase pathway and DNA binding of the transcription factor NFkappaB ... UDP-monoglucosylation and concomitant inactivation of Rho A and of Rac-2 by Clostridium difficile toxin B also inhibited IL-1 induced activation of JNK and p38 MAPK, but additionally inhibited activation of the extracellular-regulated-kinase pathway and DNA binding of the transcription factor NFkappaB
Kühn et al., Arthritis Rheum 2001 : These results suggest that CD95 dependent cell death is enhanced by NF-kappaB inhibition at and/or downstream of caspase 8 activation and that caspase 9 activation is not involved in CD95 mediated apoptosis in chondrocytes
Debets et al., J Immunol 2001 (Psoriasis) : However, IL-1epsilon activates NF-kappaB through the orphan IL-1R related protein 2 (IL-1Rrp2), whereas IL-1delta, which shows striking homology to IL-1 receptor antagonist, specifically and potently inhibits this IL-1epsilon response ... However, IL-1epsilon activates NF-kappaB through the orphan IL-1R related protein 2 (IL-1Rrp2), whereas IL-1delta, which shows striking homology to IL-1 receptor antagonist, specifically and potently inhibits this IL-1epsilon response
Bode et al., J Immunol 2001 : The inhibitory effect of IL-1 beta on IL-6 induced alpha 2-macroglobulin expression is due to activation of NF-kappa B ... The observation that inhibition of IL-6 induced transcriptional activation of this promoter by IL-1beta is reversed by cotransfection with I-kappaBalpha provides evidence that NF-kappaB activation by IL-1beta is responsible for inhibition of IL-6 mediated trans activation of the alpha(2)-macroglobulin gene ... The observation that inhibition of IL-6 induced transcriptional activation of this promoter by IL-1beta is reversed by cotransfection with I-kappaBalpha provides evidence that NF-kappaB activation by IL-1beta is responsible for inhibition of IL-6 mediated trans activation of the alpha(2)-macroglobulin gene ... Additional data are provided indicating that the activation of NF-kappaB by IL-1beta is also responsible for the inhibition of other IL-6-inducible genes, such as the alpha(1)-antichymotrypsin gene as well as the suppressor of cytokine signaling 3 gene, suggesting a more general relevance of this mechanism for transcriptional regulation
Abreu et al., J Immunol 2001 (Inflammation) : Three IEC lines ( Caco-2, T84, HT-29 ) were tested for their ability to activate an NF-kappaB reporter gene in response to purified, protein-free LPS ... Transient transfection of either gene individually was not sufficient to restore LPS signaling, but cotransfection of TLR4 and MD-2 in IEC led to synergistic activation of NF-kappaB and IL-8 reporter genes in response to LPS
Javelaud et al., Oncogene 2001 (Bone Neoplasms...) : We recently reported that inhibition of NF-kappa B activation as a consequence of the overexpression of a degradation-resistant form of I kappa B alpha [ I kappa B alpha ( A32/36 ) ] sensitized Ewing sarcoma cells to TNF alpha induced killing
Shames et al., J Surg Res 2001 : Exogenous IL-10 inhibits NF-kappaB in monocytes and thus attenuates TNF-alpha production ... We examined the role of endogenous IL-10 in the regulation of NF-kappaB activation and TNF-alpha production in human monocytes by cAMP
Fichorova et al., J Infect Dis 2001 (HIV Infections...) : Analysis of molecular and cellular components in cervicovaginal secretions, as well as results from in vitro activation of cervicovaginal epithelial cells and U1/HIV promonocytic cells, showed that multiple N-9 use can promote HIV-1 transmission through interleukin-1 mediated NF-kappaB activation, which leads to chemokine induced recruitment of HIV-1 host cells and increased HIV-1 replication in infected cells
Mochida-Nishimura et al., Mol Med 2001 (MAP Kinase Signaling System) : In this study, we compared the spontaneous and LPS stimulated activation of MAP kinases and NF-kappaB in bronchoalveolar cells ( BAC ) from smokers and nonsmokers ... LPS induced the activation of NF-kappaB in a dose dependent manner, but BAC from smokers were approximately 10 times more sensitive, and showed faster kinetics of activation of NF-kappaB than BAC from nonsmokers
Andoh et al., Clin Exp Immunol 2001 : IL-17 rapidly induced the phosphorylation and degradation of I kappa B alpha molecules, and the combination of IL-17 and IFN-gamma induced a marked increase in NF-kappa B DNA binding activity as early as 1.5 h after the stimulation
Malhotra et al., Am J Respir Cell Mol Biol 2001 : Geldanamycin inhibited TNF-alpha mediated NF-kappaB activation as measured by electromobility shift assays and transient transfections with a NF-kappaB dependent luciferase reporter plasmid
Ginis et al., Mol Med 2000 : The effect of H2O2 on TNFalpha induced activation of nuclear factor kappa B (NF-kappaB) was measured by Western blots of cytoplasmic and nuclear extracts of RBEC using anti-inhibitor of NF-kappaB ( IkappaB ) and anti-p65 subunit of NF-kappaB antibodies
Kolyada et al., Mol Med 2001 : The -1034/+88 bp iNOS promoter was strongly induced by IL-1beta, the regulatory elements for such induction being localized downstream of -205 bp. Cotransfection experiments with NF-kappaB isoforms, IkappaB isoforms, and IKK mutants suggested that the NF-kappaB site at -115/-106 bp is important, but not sufficient , for induction of iNOS promoter and that the role of NF-kappaB is partially independent of its binding site
Burmester et al., Z Rheumatol 2001 (Arthritis, Rheumatoid) : Moreover, it could be shown that gold suppresses NF-kappa B binding activity as well as the activation of the I-kappa B-kinase
Morel et al., J Biol Chem 2001 (Arthritis, Rheumatoid) : IL-18 requires NF kappa B as well as PI 3-kinase to induce VCAM-1 on RA synovial fibroblasts, suggesting that there may be two distinct pathways in IL-18 induced adhesion molecule expression ... IL-18 requires NF kappa B as well as PI 3-kinase to induce VCAM-1 on RA synovial fibroblasts, suggesting that there may be two distinct pathways in IL-18 induced adhesion molecule expression
Petranka et al., J Biol Chem 2001 : The constitutive activation of NF-kappa B observed in sup- cells is not due to loss of I kappa B alpha
Korn et al., J Biol Chem 2001 : In contrast, TNFalpha induced IKK activity rapidly and transiently resulting in IkappaBalpha degradation and NF-kappaB activation ... In contrast, TNFalpha induced IKK activity rapidly and transiently resulting in IkappaBalpha degradation and NF-kappaB activation ... Importantly, in the presence of H ( 2 ) O ( 2 ), the ability of TNFalpha to induce IKK activity was markedly decreased and resulted in prevention of IkappaBalpha degradation and NF-kappaB activation
Tada et al., J Biol Chem 2001 : Critical roles of TRAF2 and TRAF5 in tumor necrosis factor induced NF-kappa B activation and protection from cell death ... Critical roles of TRAF2 and TRAF5 in tumor necrosis factor induced NF-kappa B activation and protection from cell death ... Critical roles of TRAF2 and TRAF5 in tumor necrosis factor induced NF-kappa B activation and protection from cell death ... However, the exact roles of TRAF2 and TRAF5 in TNF induced NF-kappaB activation still remain controversial ... However, the exact roles of TRAF2 and TRAF5 in TNF induced NF-kappaB activation still remain controversial ... However, the exact roles of TRAF2 and TRAF5 in TNF induced NF-kappaB activation still remain controversial ... Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF induced NF-kappaB activation and protection from cell death ... Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF induced NF-kappaB activation and protection from cell death ... Collectively, these results indicate that both TRAF2 and TRAF5 are involved in TNF induced NF-kappaB activation and protection from cell death
Sunohara et al., J Neurochem 2001 : Induction of MARCKS and MRP by LPS was completely blocked by inhibitors of NF-kappaB ( PDTC ) and protein tyrosine kinases ( herbimycin A ), partially blocked by the p38 kinase inhibitor SB203580, and unaffected by the MEK inhibitor PD98059 ... Induction of MARCKS and MRP by LPS was completely blocked by inhibitors of NF-kappaB ( PDTC ) and protein tyrosine kinases ( herbimycin A ), partially blocked by the p38 kinase inhibitor SB203580, and unaffected by the MEK inhibitor PD98059
Khursigara et al., J Neurosci 2001 : Conversely, the expression of a dominant negative version of RIP2 protein resulted in a loss of NGF induced NF-kappaB induction and increased NGF mediated cell death
Hwang et al., Planta Med 2001 : All compounds also inhibited the LPS induced NF-kappaB activation as assessed by NF-kappaB reporter assay and electrophoretic mobility shift assay ( EMSA ) ... Compounds 2-4 showed comparable inhibitory effects on the LPS induced production of NO and PGE2, and activation of NF-kappaB without affecting cell viability
Kim et al., Neurosci Lett 2001 (Encephalitis...) : Taken together, our results indicate that wogonin inhibits activation induced death of C6 glial cells by suppressing NO production, and these inhibitory effects of wogonin on NO production are exerted through inhibition of NF-kappaB mediated iNOS induction
Han et al., J Clin Invest 2001 : TNF-alpha mediates SDF-1 alpha induced NF-kappa B activation and cytotoxic effects in primary astrocytes ... TNF-alpha mediates SDF-1 alpha induced NF-kappa B activation and cytotoxic effects in primary astrocytes ... TNF-alpha treatment mimicked SDF-1 alpha induction of NF-kappa B , IL-1 alpha/beta, and RANTES, as well as cell death ; neutralizing antibodies against TNF-alpha opposed these responses ... Late Erk1/2 activation was involved in TNF-alpha stimulated NF-kappa B activation and cytokine induction ... Late Erk1/2 activation was involved in TNF-alpha stimulated NF-kappa B activation and cytokine induction ... Late Erk1/2 activation was involved in TNF-alpha stimulated NF-kappa B activation and cytokine induction
Cinatl et al., J Immunol 2001 : Both TNF-alpha and phorbol ester 12,0-tetradecanoylphorbol 13-acetate ( TPA ) enhanced NF-kappaB activity but only TPA increased HCMV replication
Supajatura et al., J Immunol 2001 (Acute Disease...) : TLR4 mediated stimulation of mast cells by LPS was followed by activation of NF-kappaB but not by stress activated protein kinase/c-Jun NH2-terminal kinase signaling
Medvedev et al., J Immunol 2001 : Following LPS exposure, 3E10, 3E10/TLR2, and 3E10/TLR4 cells exhibited comparable decreases in LPS mediated NF-kappaB activation and mitogen activated protein ( MAP ) kinase phosphorylation ... Likewise, LPS pretreatment profoundly inhibited LPS induced NF-kappaB translocation in Chinese hamster ovary cells that concomitantly overexpressed human TLR4 and myeloid differentiation protein-2 ( MD-2 ), but failed to modulate TLR4 or MD-2 cell surface expression ... Pretreatment of 3E10/TLR2 cells with LAM or STF decreased their NF-kappaB responses induced by subsequent stimulation with these substances or LPS
Ng et al., Oncogene 2001 : Genome-wide expression changes induced by HTLV-1 Tax : evidence for MLK-3 mixed lineage kinase involvement in Tax mediated NF-kappaB activation ... Functionally, we provide evidence that one of these genes, the mixed-lineage kinase MLK-3, is involved in Tax mediated NF-kappa-B signaling
Hideshima et al., Oncogene 2001 (MAP Kinase Signaling System...) : In this study we demonstrate that tumor necrosis factor alpha (TNFalpha) triggers only modest proliferation, as well as p44/p42 mitogen activated protein kinase ( MAPK ) and NF-kappaB activation, in MM.1S multiple myeloma ( MM ) cells ... TNFalpha also activates NF-kappaB and markedly upregulates ( fivefold ) secretion of interleukin-6 (IL-6), a myeloma growth and survival factor, in bone marrow stromal cells ( BMSCs ) ... Importantly, the proteasome inhibitor PS-341 abrogated TNFalpha induced NF-kappaB activation, induction of ICAM-1 or VCAM-1, and increased adhesion of MM cells to BMSCs. Agents which act to inhibit TNFalpha may therefore abrogate the paracrine growth and survival advantage conferred by MM cell adhesion in the BM microenvironment ... Importantly, the proteasome inhibitor PS-341 abrogated TNFalpha induced NF-kappaB activation, induction of ICAM-1 or VCAM-1 , and increased adhesion of MM cells to BMSCs. Agents which act to inhibit TNFalpha may therefore abrogate the paracrine growth and survival advantage conferred by MM cell adhesion in the BM microenvironment ... Importantly, the proteasome inhibitor PS-341 abrogated TNFalpha induced NF-kappaB activation, induction of ICAM-1 or VCAM-1, and increased adhesion of MM cells to BMSCs. Agents which act to inhibit TNFalpha may therefore abrogate the paracrine growth and survival advantage conferred by MM cell adhesion in the BM microenvironment
Trajkovic et al., Glia 2001 (Central Nervous System Fungal Infections...) : The activation of transcription factor NF-kappaB was apparently required for AMB induced iNOS mRNA expression, as the latter was abolished by NF-kappaB inhibitors : pyrrolidine dithiocarbamate and MG132
Colombatti et al., Glia 2001 (Multiple Sclerosis) : We found that T-cell adhesion and T-cell soluble factors induce IL-6 gene expression in U251 astrocytes through distinct signaling pathways, respectively, resulting in the activation of NF-kappaB and IRF-1 transcription factors
Bittorf et al., Cell Signal 2001 : Activation of the transcription factor NF-kappaB by the erythropoietin receptor : structural requirements and biological significance
Marusawa et al., Microbiol Immunol 2001 : Overexpression of kinase-inactive NF-kappaB inducing kinase (NIK) and IkappaB kinase (IKK) inhibited the activation of NF-kappaB introduced by anti-Fas treatment in these cells
McMurray et al., Cytokine 2001 : The decreased nuclear binding of NFkappaB occurred in the setting of estrogen induced increases in IkappaBalpha protein levels, an important inhibitor of NFkappaB nuclear translocation
Okada et al., Cytokine 2001 : TNFR2 blockade suppressed NFkappaB1 and FLIP expression and increased cell death
Chen et al., J Cell Biochem 2001 : SME or Sal B significantly inhibited TNF-alpha induced activation of nuclear factor kappa B (NF-kappaB) in HAECs ( 0.36- and 0.48-fold, respectively ) ... SME or Sal B significantly inhibited TNF-alpha induced activation of nuclear factor kappa B (NF-kappaB) in HAECs ( 0.36- and 0.48-fold, respectively )
Guo et al., J Cell Biochem 2001 : However, expression of neither p38beta nor its dominant negative mutant in mesangial cells interfered with TNF-alpha induced translocation of NF-kappaB , the initial step of NF-kappaB activation ... While it is unclear whether p38beta regulates NF-kappaB transcription activity at other steps, it is apparent that p38beta does not affect TNF-alpha induced NF-kappaB activation at the stage of nuclear translocation ... While it is unclear whether p38beta regulates NF-kappaB transcription activity at other steps, it is apparent that p38beta does not affect TNF-alpha induced NF-kappaB activation at the stage of nuclear translocation
Liu et al., Acta Pharmacol Sin 2000 : To study the effect of triptolide on the activity of nuclear factor-kappa B (NF-kappa B) and gene expression of I kappa B alpha , a major inhibitor of NF-kappa B , in human Jurkat T cell line
Hourton et al., Eur J Biochem 2001 (Arteriosclerosis) : Treatment of macrophages with ox24hLDL suppressed the LPS induced binding of NF-kappaB to DNA ... In addition, treatment of macrophages with lysophosphatidylcholine ( 2 and 10 microM ), a major component of oxLDL, inhibited the LPS induced NF-kappaB binding to DNA and reduced PAF binding by 30 and 70 %, respectively ... In conclusion, oxLDL may downregulate PAF-R expression in human macrophages by inhibiting LPS induced NF-kappaB binding to DNA
Muta et al., Eur J Biochem 2001 : We have found that under appropriate transfection conditions, both TLR2 and TLR4 mediate LPS induced NF-kappaB activation in human embryonic kidney 293 cells ... We have found that under appropriate transfection conditions, both TLR2 and TLR4 mediate LPS induced NF-kappaB activation in human embryonic kidney 293 cells
Yuan et al., J Biol Chem 2001 : The results showed that Smad3 and Smad4, but not Smad1 or Smad2, mimicked the inhibitory effect of TGF-beta and abrogated interleukin-1beta (IL-1beta) induced stimulation of MMP-1 promoter activity and NFkappaB-specific gene transcription in dermal fibroblasts
Yang et al., Mol Pharmacol 2001 : We previously showed that green tea polyphenols inhibited endotoxin mediated tumor necrosis factor-alpha (TNF alpha) production by blocking NF-kappa B activation ... In this study, we evaluated whether green tea polyphenols inhibit NF-kappa B by blocking IKK activity ... IEC-6 cells pretreated with an extract of green tea polyphenols ( GrTPs ; 0 -- 0.4 mg/ml ) had diminished TNF alpha induced IKK and NF-kappa B activity
Brandes et al., Mol Pharmacol 2001 : In this study, we show that I kappa B alpha M expression inhibits stress induced NF-kappa B activation and prevents BFA-, hypoxia-, and OA-induced resistance to etoposide
Anderson et al., Cancer J 2001 (Multiple Myeloma...) : The upregulation of NF-kappaB dependent interleukin-6 (IL-6) transcription and secretion that occurs following adhesion of myeloma cells to bone marrow stromal cells ( BMSCs ) may serve as a potential therapeutic target, as IL-6 is a growth and survival factor for myeloma cells ... Accordingly, proteasome inhibitors inhibit activation of NF-kappaB and induce apoptosis of myeloma cells ; they also inhibit the NF-kappaB dependent up-regulation of IL-6 in BMSCs and related paracrine growth of adherent tumor cells
Szabo et al., Alcohol Clin Exp Res 2001 (Carcinoma, Hepatocellular...) : In HepG2 cells, both IFNalpha and acute alcohol treatment induced NF-kappaB activation and augmented TNFalpha induced NF-kappaB binding ... Pretreatment of HepG2 cells with IFNalpha resulted in the highest levels of NF-kappaB activation in response to TNFalpha or TNFalpha plus ethanol stimulation
Gires et al., Cancer 2001 : TNFalpha induced inhibition of the EpCAM expression is mediated by TNF receptor 1 through the TNF receptor associated death domain protein ( TRADD ) and by the activation of nuclear factor kappaB (NF-kappaB) , and it can be blocked by dominant negative variants of TRADD and the NF-kappaB inhibitor, IkappaB ... TNFalpha induced inhibition of the EpCAM expression is mediated by TNF receptor 1 through the TNF receptor associated death domain protein ( TRADD ) and by the activation of nuclear factor kappaB (NF-kappaB) , and it can be blocked by dominant negative variants of TRADD and the NF-kappaB inhibitor, IkappaB ... The authors provide further evidence that NF-kappaB represses EpCAM expression by competing for the transcriptional coactivator p300/CREB binding protein (p300/CBP)
Hu et al., Cancer Res 2001 (Leukemia, Myeloid...) : Although Bay 11-7085 prevented phorbol 12-myristate 13-acetate induced NF-kappaB nuclear translocation, SN50, a specific inhibitor of nuclear translocation and function of NF-kappaB , did not induce any significant nuclear/DNA fragmentation, caspase 3 activation, or cell death
Pati et al., J Virol 2001 (Sarcoma, Kaposi) : NF-kappaB activation was enhanced by the chemokine GROalpha, but not by IL-8
Matsumura et al., Life Sci 2001 : NF-kappaB activation by LPS/IFN was repressed by DEX
Poligone et al., J Biol Chem 2001 (Inflammation) : Positive and negative regulation of NF-kappaB by COX-2 : roles of different prostaglandins ... In this report we demonstrate COX-2 expression inhibits nuclear translocation of NF-kappaB , and we confirm that the cyclopentenone prostaglandins inhibit NF-kappaB
Majumdar et al., J Immunol 2001 : We investigated the effects of MTX on NF-kappaB activation induced by TNF in Jurkat cells ... We investigated the effects of MTX on NF-kappaB activation induced by TNF in Jurkat cells ... The treatment of these cells with MTX suppressed TNF induced NF-kappaB activation with optimum effects occurring at 10 microM MTX for 60 min ... Besides TNF, MTX also suppressed the NF-kappaB activation induced by various other inflammatory stimuli ... The suppression of TNF induced NF-kappaB activation by MTX correlated with inhibition of IkappaBalpha degradation, suppression of IkappaBalpha phosphorylation, abrogation of IkappaBalpha kinase activation, and inhibition of NF-kappaB dependent reporter gene expression ... A partial reversal of MTX induced NF-kappaB suppression by thymidine and folinic acid indicates the role of the thymidylate synthase pathway also ... Overall, our results clearly demonstrate that MTX suppresses NF-kappaB activation through the release of adenosine, which may contribute to the role of MTX in anti-inflammatory, immunomodulatory, and antiproliferative effects
Okabe et al., Biol Pharm Bull 2001 (Lung Neoplasms) : Among them, we think down-regulation of NIK gene expression is significant for cancer prevention, based on evidence that inhibition of NF-kappaB activation is a result of inhibition of NIK/IKK signalling complex
Li et al., Biochem Biophys Res Commun 2001 : To reconcile these differences we examined the effect of endogenous Tat on NF-kappaB activation, on IkappaB degradation and its interaction with upstream MAP3Ks ... Further analysis demonstrates that although Tat alone lacks direct effect on IkappaBalpha degradation or dissociation from NF-kappaB, Tat can substantially enhance the capacity of NF-kappaB inducing kinase (NIK) , but not MEKK1, to accelerate degradation of IkappaB ... Further analysis demonstrates that although Tat alone lacks direct effect on IkappaBalpha degradation or dissociation from NF-kappaB, Tat can substantially enhance the capacity of NF-kappaB inducing kinase (NIK) , but not MEKK1, to accelerate degradation of IkappaB
Roth et al., Biochim Biophys Acta 2001 : Neither was NF-kappa B-independent activation of endothelial cells by interferon-gamma influenced by homocysteine
Lührs et al., Int J Colorectal Dis 2001 (Adenocarcinoma) : The anti-inflammatory effects of butyrate may at least in part be mediated by an inhibition of IkappaBalpha mediated activation of NF-kappaB
Kim et al., J Neurosci 2001 (Spinal Cord Injuries...) : NF-kappaB transactivates c-IAP2 to inhibit caspase-3 activation
Combes et al., J Pharm Pharmacol 2001 : In view of the well documented effects of adenosine on immune and inflammatory responses, we have explored whether adenosine A1 , A2B and A3 receptors regulate NF-kappaB activity in transfected Chinese hamster ovary ( CHO ) cells using a luciferase reporter gene construct ... In conclusion, adenosine A1 , A2B and A3 receptors do not directly activate NF-kappaB in CHO cells ... However, adenosine A2B receptor activation significantly inhibited NF-kappaB activity ... However, adenosine A2B receptor activation significantly inhibited NF-kappaB activity
Bushdid et al., Dev Biol 2001 : NF-kappaB mediates FGF signal regulation of msx-1 expression ... These results suggest that NF-kappaB mediates the FGF-2/-4 signal regulation of msx-1 gene expression ... These results suggest that NF-kappaB mediates the FGF-2/-4 signal regulation of msx-1 gene expression
Qian et al., J Biol Chem 2001 : IRAK mediated translocation of TRAF6 and TAB2 in the interleukin-1 induced activation of NFkappa B
Fang et al., J Neurovirol 2001 : Induction of IFN-beta and MHC class I gene expression requires NF-kappaB activation which depends on degradation of IkappaBalpha, an inhibitory protein of NF-kappaB
Glasgow et al., Neurochem Res 2001 (Anoxia) : Transcriptional regulation of the BCL-X gene by NF-kappaB is an element of hypoxic responses in the rat brain
Tamatani et al., Cancer Lett 2001 (Carcinoma, Squamous Cell...) : Enhanced IkappaB kinase activity is responsible for the augmented activity of NF-kappaB in human head and neck carcinoma cells ... Thus, our results suggest that enhanced NF-kappaB activity in head and neck cancer cells may be due to the augmentation of IKK activity
Senftleben et al., Science 2001 : Here we show that IKKalpha is required for B cell maturation, formation of secondary lymphoid organs, increased expression of certain NF-kappaB target genes, and processing of the NF-kappaB2 ( p100 ) precursor
Akira et al., J Endotoxin Res 2000 : However, unlike the signaling mediated by other bacterial components such as lipoprotein and bacterial DNA, activation of NF-kappaB and MAP kinases was induced in response to LPS even in the absence of MyD88, which indicates the existence of a MyD88 independent pathway
Yoshimura et al., J Endotoxin Res 2000 : These findings showed that cellular activation by Gram positive cell wall components was mediated by TLR2, but not TLR4, and indicated that the glycan backbone of peptidoglycan is critical for TLR2 mediated NF-kappaB activation
Koide et al., J Endotoxin Res 2001 : TH2.52 B1 cells responded to a very low concentration of LPS and exhibited : ( i ) augmentation of DNA synthesis ; ( ii ) activation of nuclear factor (NF)-kappaB ; and ( iii ) phosphorylation of extracellular signal regulated kinase 1/2 ( Erk1/2 )
Chiao et al., AIDS Res Hum Retroviruses 2001 : Tumor necrosis factor receptor (TNFR) p55 was the prominent receptor, as neutralizing antibodies to TNFR p55, but not to TNFR p75, blocked TNF-alpha mediated NF-kappa B activation
Prösch et al., J Biol Chem 2001 : We demonstrate here that the cell differentiation dependent reduction of TNFalpha stimulation is not due to insufficient NF-kappaB activation but correlates with increased synthesis of the monocyte differentiation associated factors CCAAT/enhancer binding protein (C/EBP) alpha and beta
Cao et al., J Tongji Med Univ 2001 : To explore the effect of NF-kappa B on bcl-x gene transcription in extended drug resistance leukemia cell line HL-60/E6, drug-resistant subline HL-60/E6 was derived by intermittently exposing HL-60 cells to 6 ng/ml epirubicin ... It was concluded that NF-kappa B was involved in regulating bcl-x transcription
Horng et al., Nat Immunol 2001 : Although some cellular responses are completely abolished in MyD88-deficient mice, TLR4 , but not TLR9, can activate NF-kappa B and mitogen activated protein kinases and induce dendritic cell maturation in the absence of MyD88
Chin et al., Invest Ophthalmol Vis Sci 2001 : NF-kappa B mediated the induction of COX-2 gene expression in HRPE cells
Srahna et al., Clin Exp Immunol 2001 : NF-kappaB is involved in the regulation of CD154 ( CD40 ligand ) expression in primary human T cells
Voisard et al., BMC molecular biology 2001 : We hypothesized that tumor necrosis factor-alpha (TNF-alpha) induced and NF-kappaB mediated expression of intercellular adhesion molecule-1 ( ICAM-1 ) can be inhibited by antisense RelA p65 and NF-kappaB1 p50 oligonucleotides ( RelA p65 and NF-kappaB1 p50 ) ... We hypothesized that tumor necrosis factor-alpha (TNF-alpha) induced and NF-kappaB mediated expression of intercellular adhesion molecule-1 ( ICAM-1 ) can be inhibited by antisense RelA p65 and NF-kappaB1 p50 oligonucleotides ( RelA p65 and NF-kappaB1 p50 )
Huang et al., J Biol Chem 2001 : Chemoattractant stimulated NF-kappaB activation is dependent on the low molecular weight GTPase RhoA ... We previously demonstrated that fMet-Leu-Phe ( fMLP ) stimulates NF-kappaB activation, and this function of fMLP requires phosphatidylinositol 3-kinase (PI3K)
D'Acquisto et al., Naunyn Schmiedebergs Arch Pharmacol 2001 : Electrophoretic mobility shift assay indicated that both NO-generating agents blocked LPS induced activation of nuclear factor-kappaB (NF-kappaB) by increasing IkappaB-alpha protein expression and blocking nuclear translocation of NF-kappaB subunits p50 and p65
Druilhe et al., Cell Death Differ 2001 : Moreover, Pseudo-ICE , but not ICEBERG, interacts with the CARD containing kinase RICK/RIP2/CARDIAK and activates NF-kappaB ... Our data suggest that Pseudo-ICE and ICEBERG are intracellular regulators of caspase-1 activation and could play a role in the regulation of IL-1beta secretion and NF-kappaB activation during the pro-inflammatory cytokine response ... Our data suggest that Pseudo-ICE and ICEBERG are intracellular regulators of caspase-1 activation and could play a role in the regulation of IL-1beta secretion and NF-kappaB activation during the pro-inflammatory cytokine response
Baek et al., Eur J Immunol 2001 : Furthermore, the NF-kappaB inhibitor PDTC suppressed sPLA ( 2 ) -induced nitrite production and iNOS expression as well as IkappaBalpha degradation ... Furthermore, the NF-kappaB inhibitor PDTC suppressed sPLA ( 2 ) -induced nitrite production and iNOS expression as well as IkappaBalpha degradation
Wadsworth et al., Biochem Pharmacol 2001 : Results from western analysis, EMSA, and transient transfections suggest that EGb 761 diminishes LPS induced NF-kappaB but has no effect on LPS induced TNF-alpha transcription
Durandy et al., Curr Opin Immunol 2001 (Hypergammaglobulinemia...) : Besides the well-known role of interaction between CD40-ligand and CD40, these pathological conditions definitively demonstrate the requirement of CD40 mediated NF-kappaB activation and the essential role of a newly described molecule, activation induced cytidine deaminase ( AID ), in B cell terminal differentiation
Baetu et al., J Immunol 2001 : Disruption of NF-kappaB signaling reveals a novel role for NF-kappaB in the regulation of TNF related apoptosis inducing ligand expression ... In this T cell model, as well as in primary T lymphocytes, expression of TNF related apoptosis inducing ligand ( TRAIL ) apoptotic signaling protein was dramatically down-regulated by inhibition of NF-kappaB binding activity ... Induction of the transdominant repressor of IkappaBalpha dramatically down-regulated surface expression of TRAIL, indicating an essential role for NF-kappaB in the regulation of TRAIL ... The regulation of TRAIL expression by NF-kappaB may represent a general mechanism that contributes to the control of TRAIL mediated apoptosis in T lymphocytes
Prebeck et al., J Immunol 2001 : Importantly, secretion of cytokines as well as translocation of NF-kappaB were dependent on the presence of TLR2 and independent from TLR4 with the exception of IL-12p40 secretion, which was attenuated in the absence of either a functional TLR2 or 4
Ohnishi et al., J Immunol 2001 : N-linked glycosylations at Asn ( 26 ) and Asn ( 114 ) of human MD-2 are required for toll-like receptor 4-mediated activation of NF-kappaB by lipopolysaccharide ... Although these mutants were expressed on the cell surface and maintained its ability to associate with human TLR4, these mutations or tunicamycin treatment substantially impaired the ability of MD-2 to complement TLR4 mediated activation of NF-kappaB by LPS ... Although these mutants were expressed on the cell surface and maintained its ability to associate with human TLR4, these mutations or tunicamycin treatment substantially impaired the ability of MD-2 to complement TLR4 mediated activation of NF-kappaB by LPS
Oshima et al., Am J Physiol Cell Physiol 2001 (Inflammatory Bowel Diseases...) : Similarly, TNF-alpha activated NF-kappa B through TK, p42/44, p38 MAPKs, and PARP pathways in SVEC cells ... MAdCAM-1 expression requires NF-kappa B translocation through both direct p42/44 and indirect p38 MAPK pathways in high endothelial cells
Maeda et al., J Biol Chem 2001 (Stomach Neoplasms) : In conclusion, H. pylori induced NF-kappaB activation in epithelial cells is dependent on cag PAI and contact but does not involve CD14 and IRAK, whereas in macrophage/monocytic cells it is independent of cag PAI or contact but involves CD14 and TLR4 ... In conclusion, H. pylori induced NF-kappaB activation in epithelial cells is dependent on cag PAI and contact but does not involve CD14 and IRAK , whereas in macrophage/monocytic cells it is independent of cag PAI or contact but involves CD14 and TLR4 ... In conclusion, H. pylori induced NF-kappaB activation in epithelial cells is dependent on cag PAI and contact but does not involve CD14 and IRAK, whereas in macrophage/monocytic cells it is independent of cag PAI or contact but involves CD14 and TLR4
Kitaura et al., FEMS Immunol Med Microbiol 2001 (HIV Infections...) : These findings indicated that activation of Mycobacterium induced LTR CAT is NF-kappaB dependent ... These findings suggested that activation of HIV-1 LTR by mycobacteria was mainly mediated by NF-kappaB induced secondary release of cytokine TNF-alpha
Kanamaru et al., J Biol Chem 2001 : We also demonstrated that the induction of Smad7 by NE is dependent on nuclear factor-kappa B (NF-kappa B)
Jana et al., J Biol Chem 2001 : However, CD40 ligation alone induced the activation of only NF-kappaB but not of C/EBPbeta, suggesting that the activation of NF-kappaB alone by CD40 ligation is not sufficient to induce the expression of iNOS and that the activation of C/EBPbeta is also necessary for the expression of iNOS ... However, CD40 ligation alone induced the activation of only NF-kappaB but not of C/EBPbeta, suggesting that the activation of NF-kappaB alone by CD40 ligation is not sufficient to induce the expression of iNOS and that the activation of C/EBPbeta is also necessary for the expression of iNOS ... Stimulation of IFN-gamma mediated activation of NF-kappaB but not of C/EBPbeta by CD40 ligation suggests that CD40 ligation stimulates the expression of iNOS in IFN-gamma treated BV-2 microglial cells through the stimulation of NF-kappaB activation ... Stimulation of IFN-gamma mediated activation of NF-kappaB but not of C/EBPbeta by CD40 ligation suggests that CD40 ligation stimulates the expression of iNOS in IFN-gamma treated BV-2 microglial cells through the stimulation of NF-kappaB activation
Bouchier-Hayes et al., J Biol Chem 2001 : Rather, CARDINAL potently suppressed NF-kappaB activation associated with overexpression of TRAIL-R1, TRAIL-R2, RIP, RICK, Bcl10, and TRADD, or through ligand induced stimulation of the interleukin-1 or tumor necrosis factor receptors
Koay et al., Infect Immun 2001 : LPS induced NF-kappaB binding activity and accumulation of RelA in nuclear protein extracts of lung tissue were markedly increased in WT compared to p47phox-/- mice 90 min after treatment with 20 but not 5 microg of i.p. LPS per g ... We conclude that LPS induced NF-kappaB activation is deficient in the lungs of p47phox-/- mice compared to WT mice, but this abnormality does not result in overt alteration in the acute inflammatory response
Jarosinski et al., Lab Invest 2001 : However, long-term exposure to translational inhibitors resulted in IkappaBalpha decay and activation of latent NF-kappaB in neurons, indicating potential NF-kappaB activity in these cells
Blais et al., J Neuropathol Exp Neurol 2001 : Inhibitory action of nitric oxide on circulating tumor necrosis factor induced NF-kappaB activity and COX-2 transcription in the endothelium of the brain capillaries ... This study investigated the hypothesis that nitric oxide ( NO ) acts as an endogenous modulator of TNF induced NF-kappaB signaling and COX-2 transcription in the endothelium of the cerebral capillaries ... These results indicate that eNOS derived NO acts as an endogenous inhibitor of TNF-alpha induced NF-kappaB activity and COX-2 transcription in the endothelium of the cerebral capillaries
Zhang et al., J Biol Chem 2001 : Inhibition of cell cycle progression by the novel cyclophilin ligand sanglifehrin A is mediated through the NFkappa B-dependent activation of p53
Siegmund et al., J Biol Chem 2001 : As both TNF and PMA rapidly induce NF-kappaB activation this suggests that NEMO/IKKgamma dependent activation of the NF-kappaB pathway is necessary but not sufficient for up-regulation of TRAIL in T cells
Chandel et al., J Biol Chem 2001 : Consistent with its anti-apoptotic function, Bcl-x ( L ) interfered with TRAF mediated ROS generation but not NF-kappaB activation
Schiffer et al., J Clin Invest 2001 (Disease Models, Animal...) : Unlike TGF-beta, Smad7 inhibits nuclear translocation and transcriptional activity of the cell survival factor NF-kappaB
Saile et al., Eur J Cell Biol 2001 : TGF-beta as well as TNF-alpha induced activation of NFKB and upregulated bcl-xL
Amrani et al., Mol Pharmacol 2001 : SB203580 and PD98059 had little effect on TNF alpha induced nuclear factor-kappa B (NF-kappa B) activation as determined in cells transfected with a NF-kappa B-luciferase reporter construct
Zhen et al., Mol Pharmacol 2001 : Activation of NF-kappa B by 1 microM PD168077 was attenuated in D ( 4 ) MN9D cells that were transfected with a kinase-deficient Akt but not in cells transfected with a dominant negative Ras ( N17Ras ), suggesting that NF-kappa B activation requires Akt but is independent of Ras ... All these indicate that distinct mechanisms mediate ERK and Akt/NF-kappa B activation by D(4) dopamine receptor stimulation
Torrie et al., Br J Pharmacol 2001 : 2. Expression of dominant negative ( DN ) -IKK-alpha, IKK-beta and NF-kappaB inducing kinase (NIK) abolished LPS stimulated NF-kappaB reporter activity, suggesting that activation of a NIK/IKK dependent pathway is indispensable for NF-kappaB activation by LPS in this cell type ... Preincubation of RASMC with H ( 2 ) O ( 2 ) inhibited LPS stimulated IKK kinase activity and downstream NF-kappaB-DNA binding activity ... Effective inhibition of this pathway using SB203580 did not reverse the effects of H ( 2 ) O ( 2 ) on LPS stimulated IKK/NF-kappaB signalling ... 5. These studies show that hydrogen peroxide mediated inhibition of LPS stimulated NF-kappaB activation in RASMC occurs upstream of IKK
Philip et al., J Biol Chem 2001 (Melanoma...) : To our knowledge, this is the first report that MMP-2 plays a direct role in OPN induced cell migration, invasion, and tumor growth and that demonstrates that OPN stimulated MMP-2 activation occurs through NF-kappaB mediated induction of MT1-MMP
Ashburner et al., Mol Cell Biol 2001 : HDAC2 does not interact with NF-kappaB directly but can regulate NF-kappaB activity through its association with HDAC1
Kim et al., J Biol Chem 2001 : SNIP1 inhibits NF-kappa B signaling by competing for its binding to the C/H1 domain of CBP/p300 transcriptional co-activators ... In this report we show that SNIP1 potently inhibits the activity of NF-kappa B , which binds the C/H1 domain of CBP/p300, but does not interfere with the activity of transcription factors such as p53, which bind to other domains of p300, or factors such as VP16, which are independent of these co-activators
Kurland et al., J Biol Chem 2001 : We investigated the role of NF-kappaB1 ( p50 ) homodimers in the expression of Bcl-2 in two murine B-cell lymphoma cell lines : LY-as, an apoptosis-proficient line with low Bcl-2 protein expression and no nuclear NF-kappaB activity, and LY-ar , a nonapoptotic line with constitutive p50 homodimer activity and 30 times more Bcl-2 protein expression than LY-as ... We investigated the role of NF-kappaB1 ( p50 ) homodimers in the expression of Bcl-2 in two murine B-cell lymphoma cell lines : LY-as, an apoptosis-proficient line with low Bcl-2 protein expression and no nuclear NF-kappaB activity, and LY-ar, a nonapoptotic line with constitutive p50 homodimer activity and 30 times more Bcl-2 protein expression than LY-as
Kim et al., Parasitol Res 2001 (Toxoplasmosis) : An inhibitor of NF-kappaB activation, calpain-1 inhibitor , blocked the chemokine secretion induced by live T. gondii ... These results suggest that NF-kappaB is a central regulator of the chemokine response in T. gondii infected human epithelial cells and that chemokine IL-8 and MCP-1 secretion might be involved in the pathogenesis of T. gondii, via the recruitment of neutrophils, monocytes, and lymphocytes ... These results suggest that NF-kappaB is a central regulator of the chemokine response in T. gondii infected human epithelial cells and that chemokine IL-8 and MCP-1 secretion might be involved in the pathogenesis of T. gondii, via the recruitment of neutrophils, monocytes, and lymphocytes
Koppinen et al., Exp Cell Res 2001 : When Edar was expressed at low levels, the NF-kappaB response was enhanced by coexpression of ectodysplasin
Richter et al., J Biol Chem 2001 : The antigen presenting capacity of TNF-alpha stimulated CD34 ( + ) cells was strongly inhibited by ICOSIg fusion proteins or by NF-kappa B inhibition
Melotti et al., Gene Ther 2001 (Cystic Fibrosis...) : All these inhibitors abolished both Ad.CFTR induced NF-kB DNA binding and transactivating activities
Yin et al., J Biol Chem 2001 : Using confocal microscopy and cell fractionation studies, butyrate pretreatment of a human colon cell line ( HT-29 cells ) inhibited the tumor necrosis factor-alpha (TNF-alpha) induced nuclear translocation of the proinflammatory transcription factor NF-kappaB
Hanausek et al., Proc Natl Acad Sci U S A 2001 (Aneuploidy...) : These findings, when combined with the proapoptotic property of these compounds and their ability to inhibit hydrogen peroxide ( H ( 2 ) O ( 2 ) ) generation, nuclear factor-kappaB (NF-kappaB) activation, and inducible nitric oxide synthase (iNOS) induction reported elsewhere, suggest that avicins could prove exciting in reducing oxidative and nitrosative stress and thereby suppressing the development of human skin cancer and other epithelial malignancies
Haridas et al., Proc Natl Acad Sci U S A 2001 : Both F094 and avicin G were found to be potent inhibitors of TNF induced NF-kappaB
Liu et al., J Cell Biochem 2001 : Distinct involvement of NF-kappaB and p38 mitogen activated protein kinase pathways in serum deprivation mediated stimulation of inducible nitric oxide synthase and its inhibition by 4-hydroxynonenal ... Cytokine induced expression of inducible nitric oxide synthase (iNOS) and concomitant production of nitric oxide ( NO ) involve activation of mitogen activated protein ( MAP ) kinases and are in most cases mediated by the transcription factor NF-kappaB ... Cytokine induced expression of inducible nitric oxide synthase (iNOS) and concomitant production of nitric oxide ( NO ) involve activation of mitogen activated protein ( MAP ) kinases and are in most cases mediated by the transcription factor NF-kappaB ... Taken together, these results suggest that NF-kappaB and p38 MAP kinase lie in separate signal pathways for serum deprivation stimulated iNOS expression and NO production
Heimberg et al., Diabetes 2001 : The transcription factor nuclear factor-kappaB (NF-kappaB) is activated by interleukin-1beta (IL-1beta) , and its activity promotes the expression of several beta-cell genes, including pro- and anti-apoptotic genes
Zhao et al., J Biol Chem 2001 (Colonic Neoplasms...) : By using reporter gene constructs containing targeted substitutions in the IL-8 promoter, we show that the NF-kappaB , AP-1, and to a lesser degree the C/EBP sites in the IL-8 promoter region are required for IL-8 gene expression induced by NT
Lührs et al., Dig Dis Sci 2001 (Translocation, Genetic) : Extracellular stimuli, notably interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) activate NF-kappaB nuclear translocation via IkappaB phosphorylation and degradation ... Extracellular stimuli, notably interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) activate NF-kappaB nuclear translocation via IkappaB phosphorylation and degradation ... In cells pretreated with butyrate, a time- and dose dependent inhibition of IL-1beta mediated NF-kappaB nuclear translocation was observed ... These data indicate that inhibition of IL-1beta induced NF-kappaB activation by butyrate does not require an intact IkappaB alpha protein
Philpott et al., Eur J Cancer 2001 : TNF was measured by an enzyme linked immunosorbent assay after 8 h, and NF-kappaB induction by electrophoretic mobility shift assays ( EMSA ) after 2 h. DMXAA ( 800 microg/ml ) had no effect alone on TNF production but augmented , by up to 4-fold, the ability of bacterial lipopolysaccharide (LPS) to induce TNF
Chang et al., J Biol Chem 2001 : Here, we report the molecular mechanism by which PARP-1 activates the sequence-specific binding of NF-kappaB to its oligodeoxynucleotide
Mikita et al., J Biol Chem 2001 : OxLDL pretreatment resulted in a significant perturbation of LPS induced NF kappa B activation
Mendes et al., Mediators Inflamm 2001 : We also studied the ability of DPI to prevent IL-1 induced NF-kappaB activation and reactive oxygen species ( ROS ) production ... These results also support the involvement of reactive oxygen species in IL-1 induced NF-kappaB activation and expression of NF-kappaB dependent genes, such as iNOS
Bruce-Keller et al., J Neurochem 2001 : Inhibitor studies revealed that Tat induced NF kappa B activation was responsible for increased nitrite release, while MAPK activation mediated superoxide release, TNF alpha release, and phagocytosis
Neff et al., Cell Microbiol 2001 (Arthritis, Rheumatoid...) : Our experiments also indicated that stimulation of FLSs with protein I/II induces nuclear translocation of NF-kappaB, AP-1 binding activity and that NF-kappaB plays a major role in IL-6 and IL-8 secretion from activated cells ... Our experiments also indicated that stimulation of FLSs with protein I/II induces nuclear translocation of NF-kappaB, AP-1 binding activity and that NF-kappaB plays a major role in IL-6 and IL-8 secretion from activated cells
Kim et al., J Neurovirol 2001 : Additionally, ts1 infection of astrocytes in vitro increased expression of inducible nitric oxide synthase (iNOS) , a NF-kappaB dependent gene product
Cooke et al., Biochem J 2001 : Functional analysis of the interleukin-1-receptor associated kinase ( IRAK-1 ) in interleukin-1 beta stimulated nuclear factor kappa B (NF-kappa B) pathway activation : IRAK-1 associates with the NF-kappa B essential modulator (NEMO) upon receptor stimulation ... The interleukin-1 (IL-1)-receptor associated kinase ( IRAK-1 ) is essential for IL-1 stimulated nuclear factor kappa B (NF-kappa B) activation ... However, an IRAK-1 variant lacking only the N-terminal domain retained the ability of the full-length protein to potentiate both IL-1 beta and tumour necrosis factor alpha (TNF alpha) induced NF-kappa B activation ... Expression of an IRAK-1 variant lacking only the C-terminal domain preferentially inhibited IL-1 beta versus TNF alpha induced NF-kappa B activation
Lakshminarayanan et al., Am J Pathol 2001 (Ischemia) : These results suggest that reactive oxygen intermediate generation, after a brief ischemic episode, is capable of inducing MCP-1 expression in venular endothelium through AP-1 and NF-kappaB
Stanimirovic et al., J Neuroimmunol 2001 (Hypoxia, Brain) : IL-1 receptor antagonist (IL-1Ra) blocked both hypoxic astrocyte conditioned media induced NF-kappaB activation and the expression/release of IL-1beta and IL-8 ... The data suggest that hypoxia/reoxygenation induced up-regulation of IL-1beta and IL-8 in human astrocytes has two components, a NF-kappaB independent up-regulation during hypoxia, followed by amplification through autocrine IL-1beta induced NF-kappaB activation during reoxygenation
Chakraborty et al., Cancer Res 2001 (Neoplasms, Hormone-Dependent...) : Neither Fas pathway activation alone nor inhibition of NF-kappaB activity with IkappaB-super repressor was sufficient to induce apoptosis of prostate cancer cells
Yeung et al., Mol Cell Biol 2001 : Raf kinase inhibitor protein interacts with NF-kappaB inducing kinase and TAK1 and inhibits NF-kappaB activation ... Raf kinase inhibitor protein interacts with NF-kappaB inducing kinase and TAK1 and inhibits NF-kappaB activation ... Modulation of RKIP expression levels affected NF-kappaB signaling independent of the MAPK pathway ... RKIP physically interacted with four kinases of the NF-kappaB activation pathway, NF-kappaB inducing kinase, transforming growth factor beta activated kinase 1, IKKalpha, and IKKbeta ... RKIP physically interacted with four kinases of the NF-kappaB activation pathway, NF-kappaB inducing kinase, transforming growth factor beta activated kinase 1, IKKalpha , and IKKbeta ... RKIP physically interacted with four kinases of the NF-kappaB activation pathway, NF-kappaB inducing kinase, transforming growth factor beta activated kinase 1, IKKalpha, and IKKbeta
Nicot et al., Mol Cell Biol 2001 : In addition, inhibition of Tax mediated NF-kappaB activation by coexpression of IkappaBalpha restored c-Myb transcription, and Tax was unable to block c-Myb transcription in a NEMO knockout cell line ... In addition, inhibition of Tax mediated NF-kappaB activation by coexpression of IkappaBalpha restored c-Myb transcription, and Tax was unable to block c-Myb transcription in a NEMO knockout cell line
Shepard et al., J Biol Chem 2001 (Sarcoma, Kaposi) : Constitutive activation of NF-kappa B and secretion of interleukin-8 induced by the G protein coupled receptor of Kaposi 's sarcoma associated herpesvirus involve G alpha(13) and RhoA ... Constitutive activation of NF-kappa B and secretion of interleukin-8 induced by the G protein coupled receptor of Kaposi 's sarcoma associated herpesvirus involve G alpha(13) and RhoA ... Constitutive activation of NF-kappa B and secretion of interleukin-8 induced by the G protein coupled receptor of Kaposi 's sarcoma associated herpesvirus involve G alpha(13) and RhoA
Méndez-Samperio et al., Cell Immunol 2001 : Roles of intracellular calcium and NF-kappaB in the Bacillus Calmette-Guérin induced secretion of interleukin-8 from human monocytes
Rani et al., J Biol Chem 2001 : A role for NF-kappa B in the induction of beta-R1 by interferon-beta
Cheng et al., J Immunol 2001 : Notch-1 regulates NF-kappaB activity in hemopoietic progenitor cells
Li et al., J Neurosci Res 2001 (MAP Kinase Signaling System) : Such antiinflammatory effects of vitamin E were correlated with suppression of p38 MAPK and NFkappaB activation and were mimicked by an inhibition of either p38 MAPK ( by SB203580 ) or NFkappaB ( by decoy oligonucleotides )
Lindholm et al., Clin Exp Metastasis 2000 (Neoplasm Invasiveness...) : The PC-3 High Invasive cells showed a constitutive increase in phospho-IkappaB alpha and introduction of the super-repressor IkappaB alpha S32/36A inhibited NF-kappaB activity to 19.2 +/- 2.5 percent of control transfected cells ( P < or = 0.001 )
Um et al., Oncogene 2001 : Furthermore, PKA inhibitor suppressed markedly the constitutive and drug induced activities of NF-kappaB and Ku in MDR cells and subsequently potentiated the cytotoxic activity of anticancer drugs
Zetoune et al., Cytokine 2001 : A20, a TNF inducible gene, inhibits TNF mediated apoptosis as well as NF-kappa B induced by this cytokine ... Similarly, the NF-kappa B inducing activity of TAX , an activator of the I kappa B kinase complex, is also abrogated by A20
Kim et al., Am J Physiol Lung Cell Mol Physiol 2001 : ZVAD inhibited the activation of extracellular signal regulated kinase ( ERK ) and p38 but not of nuclear factor (NF)-kappa B induced by LPS , suggesting that the ZVAD-sensitive molecule lies upstream of the ERK and p38 pathways but downstream of the divergent site of NF-kappa B and mitogen activated protein kinases
Schultheiss et al., EMBO J 2001 (Cell Transformation, Viral...) : In addition, NF-kappaB but not c-Jun N-terminal kinase 1 ( JNK1 ) induction by LMP1 involves TRAF6
Morel et al., Lab Invest 2001 (Arthritis, Rheumatoid) : Interleukin-18 induces rheumatoid arthritis synovial fibroblast CXC chemokine production through NFkappaB activation ... By immunofluorescence and Western blot, we demonstrated that IL-18 activates the translocation of the transcription factor nuclear factor kappa B (NFkappaB) into the nucleus of RA synovial fibroblasts ... IL-18 induces IL-8 secretion through NFkappaB because RA synovial fibroblasts pretreated with antisense to NFkappaB p65 oligonucleotide produce a mean of 44 % less IL-8 compared with cells pretreated with the control sense oligonucleotide ... IL-18 induces IL-8 secretion through NFkappaB because RA synovial fibroblasts pretreated with antisense to NFkappaB p65 oligonucleotide produce a mean of 44 % less IL-8 compared with cells pretreated with the control sense oligonucleotide
Cowling et al., J Biol Chem 2002 : The use of pharmacologic inhibitors and a degradation-resistant mutant I kappa B-alpha demonstrated that the transcription factor nuclear factor-kappa B (NF-kappa B) is necessary for cytokine induced AT(1) up-regulation ... These results suggest that low but persistent IKK activity and I kappa B degradation lead to prolonged NF-kappa B nuclear translocation and maximal AT(1) up-regulation in the continued presence of TNF-alpha ... These results suggest that low but persistent IKK activity and I kappa B degradation lead to prolonged NF-kappa B nuclear translocation and maximal AT(1) up-regulation in the continued presence of TNF-alpha
Kolonics et al., Cell Signal 2001 (MAP Kinase Signaling System) : The GM-CSF promoted cell survival and proliferation correlated with MEK-1 dependent ERK1/2, Elk-1 and CREB phosphorylation and Egr-1, c-Fos expression as well as with increased STAT-5, AP-1, c-Myb and NF-kappaB DNA binding
Grumont et al., J Exp Med 2001 : This data establishes the IL-12 p35 gene as a new target of c-Rel and shows that the regulation of IL-12 p70 expression at the transcriptional level by Rel/NF-kappaB is controlled through both the p35 and p40 genes in a cell type-specific fashion ... This data establishes the IL-12 p35 gene as a new target of c-Rel and shows that the regulation of IL-12 p70 expression at the transcriptional level by Rel/NF-kappaB is controlled through both the p35 and p40 genes in a cell type-specific fashion
Shao et al., Cancer Res 2001 (Ovarian Neoplasms) : To test whether Akt is regulated by E1A and is involved in radiation induced NF-kappaB activity, we examined the phosphorylation status of Akt in the E1A transfectants and parental cells and in irradiated cells ... We found that dominant negative Akt and wortmannin did not block radiation induced NF-kappaB activity
Bradbury et al., Cancer Res 2001 : Recently, it also has been demonstrated that nonsteroidal anti-inflammatory agents inhibit tumor necrosis factor and interleukin-1 induced NF-kappaB activation and act as radiosensitizing agents ... In addition, the transient genetic activation of p38 ( MAPK ) inhibited IR induction of NF-kappaB gene expression in the absence of indomethacin ... In addition, the transient genetic activation of p38 ( MAPK ) inhibited IR induction of NF-kappaB gene expression in the absence of indomethacin
Masamune et al., J Biol Chem 2002 : 15d-PGJ2, but not troglitazone, inhibited the degradation of IkappaB-alpha and consequent NF-kappaB activation
Alexopoulou et al., Nature 2001 (Virus Diseases) : Recognition of double stranded RNA and activation of NF-kappaB by Toll-like receptor 3 ... Here we show that mammalian TLR3 recognizes dsRNA, and that activation of the receptor induces the activation of NF-kappaB and the production of type I interferons ( IFNs )
Sharma et al., J Appl Physiol 2001 : We investigated the effects of E2 treatment and adenovirus mediated estrogen receptor (ER)-alpha gene transfer on cell proliferation and NF-kappaB activation using VSM cells cultured from 3-mo-old and 24-mo-old Fischer 344 female rats ... Constitutive and interleukin (IL)-1beta stimulated NF-kappaB activation was significantly greater ( P < 0.05 ) in VSM cells from aged compared with young female rats ... Constitutive and interleukin (IL)-1beta stimulated NF-kappaB activation was significantly greater ( P < 0.05 ) in VSM cells from aged compared with young female rats ... E2 treatment of VSM cells from aged female rats inhibited both constitutive and IL-1beta stimulated NF-kappaB activation ... In conclusion, our data demonstrate that constitutive and IL-1beta stimulated NF-kappaB activation is increased in VSM cells from aged female rats due to loss of E2 and this can be restored back to normal levels by ER-alpha gene transfer and E2 treatment ... In conclusion, our data demonstrate that constitutive and IL-1beta stimulated NF-kappaB activation is increased in VSM cells from aged female rats due to loss of E2 and this can be restored back to normal levels by ER-alpha gene transfer and E2 treatment
Yasui et al., Thromb Res 2001 : IL-1beta stimulation caused activation of nuclear factor-kappaB (NF-kappaB) and expression of cyclooxygenase-2 (COX-2) mRNA and protein, which were inhibited by daunorubicin
Fiumara et al., Blood 2001 (Hodgkin Disease) : Previous studies reported that receptor activator of nuclear factor kappaB (NF-kappaB) ( RANK ), a member of the TNF receptor family, is expressed on DCs, and that RANK ligand (RANKL) enhances DC survival and induces them to secrete cytokines ... Soluble RANKL activated NF-kappaB and induced messenger RNA expression of interferon-gamma, interleukin-8 (IL-8), IL-13, IL-9, IL-15, and RANTES, in addition to the receptors for IL-9, IL-13, IL-15, and CCR4
Yi et al., Int Immunol 2001 : CpG DNA and LPS synergistically induce TNF-alpha production in RAW264.7 cells and J774 cells through activation of NF-kappaB ... CpG DNA and LPS synergistically induce TNF-alpha production in RAW264.7 cells and J774 cells through activation of NF-kappaB ... Furthermore, transient transfection with a super-repressive mutant of IkappaBalpha ( IkappaBalpha-AA ) demonstrated that NF-kappaB plays a critical role in CpG DNA mediated TNF-alpha expression
Ching et al., Biochem Biophys Res Commun 2001 : BRE, a putative stress modulating gene, found able to down-regulate TNF-alpha induced NF-kappaB activation upon overexpression, is now shown in human cells expressed as multiple mRNA isoforms
Qin et al., J Invest Dermatol 2001 : These results indicate that interferon-gamma and TNF-alpha synergistically induce keratinocyte apoptosis when concomitant induction of NF-kappaB is blocked ... These results indicate that interferon-gamma and TNF-alpha synergistically induce keratinocyte apoptosis when concomitant induction of NF-kappaB is blocked
Cosgaya et al., J Neurochem 2001 : Binding of nerve growth factor to its p75 receptor in stressed cells induces selective IkappaB-beta degradation and NF-kappaB nuclear translocation ... In the present study we found that NGF binding to p75 induces nuclear translocation of p65 and increases NF-kappaB binding activity in a cell line overexpressing p75, but only after the cells have been subjected to a previous stress
Seo et al., Gen Pharmacol 2000 : Inhibition of NO production was achieved by reducing inducible nitric oxide synthase (iNOS) expression at protein and mRNA levels and by inactivating nuclear factor-kappa B (NF-kappa B) , but not by inhibiting iNOS activity
Hayashi et al., Neurol Res 2001 (Carotid Artery Injuries...) : Immunohistochemical and Western blotting studies demonstrated that NAC suppressed the injury induced NF-kappaB activity in the medial SMC layer
Kim et al., Dig Dis Sci 2001 : An NF-kappaB inhibitor ( pyrrolidine dithiocarbamate ), a MAP kinase ( MEK ) inhibitor ( PD98059 ), and a p38 MAP kinase inhibitor ( SB203580 ) significantly suppressed the COX-2 gene transcription and PGE2 synthesis in the neutrophils
Kamata et al., FEBS Lett 2001 : Here we studied the effect of cPrG.HCl on TNFalpha induced activation of the transcription factor nuclear factor kappaB (NF-kappaB) ... Here we studied the effect of cPrG.HCl on TNFalpha induced activation of the transcription factor nuclear factor kappaB (NF-kappaB) ... cPrG.HCl inhibited neither the TNFalpha induced phosphorylation and degradation of inhibitor of nuclear factor-kappaB, nor the subsequent nuclear translocation and DNA binding of NF-kappaB ... cPrG.HCl inhibited neither the TNFalpha induced phosphorylation and degradation of inhibitor of nuclear factor-kappaB, nor the subsequent nuclear translocation and DNA binding of NF-kappaB
Chen et al., Br J Pharmacol 2001 : We have previously reported that G protein coupled P2Y(6) receptor activation by UTP potentiates lipopolysaccharide (LPS) induced I kappa B phosphorylation and degradation, and NF-kappa B activation in J774 macrophages ... 2. In murine J774 macrophages, LPS induced NF-kappa B activation was inhibited by the presence of PDTC, D609, Ro 31-8220, PD 098059 and SB 203580
Arcaroli et al., Clin Immunol 2001 (Endotoxemia...) : Similarly, the increased production of proinflammatory cytokines and activation of NF-kappaB in lung neutrophils induced by hemorrhage or endotoxemia was not diminished by p38 inhibition
Thomas et al., J Biol Chem 2002 : Finally, both PI 3-K inhibition by LY294002 and AKT inhibition by transfection of a dominant negative enzyme blocked RSV induced NF-kappaB transcriptional activity ... Finally, both PI 3-K inhibition by LY294002 and AKT inhibition by transfection of a dominant negative enzyme blocked RSV induced NF-kappaB transcriptional activity
Solan et al., J Biol Chem 2002 : Thus, these studies indicate that p100 is a bone fide inhibitor of RelB and that this transcription factor may be regulated by NF-kappaB inducing kinase and/or IKKalpha
Liu et al., Ann Clin Lab Sci 2001 (Cholestasis...) : NF-KB , in turn, initiates the transcription of cytokine genes ( eg, IL-6 , IL-8, TNF-alpha ), which triggers hepatic injury, at least in part, by a free radical mediated apoptotic mechanism
Afford et al., FASEB J 2001 (Liver Cirrhosis, Biliary) : CD40 activation induced , Fas dependent apoptosis and NF-kappaB/AP-1 signaling in human intrahepatic biliary epithelial cells ... CD40 activation induced, Fas dependent apoptosis and NF-kappaB/AP-1 signaling in human intrahepatic biliary epithelial cells
Zhang et al., FASEB J 2001 : Alpha-lipoic acid inhibits TNF-alpha induced NF-kappaB activation and adhesion molecule expression in human aortic endothelial cells ... Furthermore, LA dose-dependently inhibited TNF-alpha induced IkappaB kinase activation, subsequent degradation of IkappaB, the cytoplasmic NF-kappaB inhibitor, and nuclear translocation of NF-kappaB ... In contrast, TNF-alpha induced NF-kappaB activation and adhesion molecule expression were not affected by ascorbic acid or by manipulating cellular glutathione status with l-2-oxo-4-thiazolidinecarboxylic acid, N-acetyl-l-cysteine, or d, l-buthionine-S, R-sulfoximine
Sliva et al., J Biol Chem 2002 (Breast Neoplasms...) : The urokinase-type plasminogen activator ( uPA ) promoter contains an NF-kappaB binding site, and uPA expression in MDA-MB-231 cells is induced by the constitutively active NF-kappaB ... Although wortmannin, Ly294002, or overexpression of p85DN did not significantly reduce DNA binding activity of NF-kappaB in nuclear extracts, wortmannin, Ly294002, and the overexpression of p85DN or IkappaBalpha inhibited constitutive activation of NF-kappaB in a reporter gene assay ... Although wortmannin, Ly294002, or overexpression of p85DN did not significantly reduce DNA binding activity of NF-kappaB in nuclear extracts, wortmannin, Ly294002, and the overexpression of p85DN or IkappaBalpha inhibited constitutive activation of NF-kappaB in a reporter gene assay ... Furthermore, inhibition of NF-kappaB markedly attenuated endogenous migration, and inhibition of PI 3-kinase and NF-kappaB reduced secretion of uPA
Bernal et al., Blood 2001 (Leukemia, Lymphocytic, Chronic, B-Cell) : The response includes inhibition of caspase activity, activation of NF-kappaB , and expression of mcl-1, bcl-2, and bfl-1 in the tumor cells
Busch et al., J Immunol 2001 : However, studies of transiently overexpressed LMP1 molecules, primarily in epithelial cells, indicated that a second region, CTAR2, is largely responsible for LMP1 mediated activation of NF-kappaB and c-Jun N-terminal kinase
Smith et al., J Immunol 2001 (Arthritis, Rheumatoid) : NF-kappaB inducing kinase is dispensable for activation of NF-kappaB in inflammatory settings but essential for lymphotoxin beta receptor activation of NF-kappaB in primary human fibroblasts
Ferlito et al., J Leukoc Biol 2001 (Leukemia, Monocytic, Acute...) : In naive cells, LPS, TNF-alpha , and IL-1beta induced IkappaBalpha degradation, kinase phosphorylation, and NF-kappaB DNA binding ... In naive cells, LPS, TNF-alpha, and IL-1beta induced IkappaBalpha degradation, kinase phosphorylation, and NF-kappaB DNA binding ... In naive cells, LPS , TNF-alpha, and IL-1beta induced IkappaBalpha degradation, kinase phosphorylation, and NF-kappaB DNA binding ... These data demonstrate that IL-1beta induces cross-tolerance to LPS induced mediator production without suppressing LPS induced signaling to MAP kinases or NF-kappaB activation
DiDonato et al., Science's STKE : signal transduction knowledge environment 2001 : In B cell maturation, conventional NF-kappaB signal induced activation of IKK alpha results in phosphorylation of p100 precursor proteins and increased proteolytic processing and constitutive NF-kappaB activation
Chan et al., Shock 2001 : To determine the role of the human coronary artery endothelial cell ( HCA-EC ) in the cytokine response to endotoxin we measured in vitro TNF-alpha synthesis, TNF-alpha mRNA, and the associated NF-kappa B response to LPS ... To determine the role of the human coronary artery endothelial cell ( HCA-EC ) in the cytokine response to endotoxin we measured in vitro TNF-alpha synthesis, TNF-alpha mRNA, and the associated NF-kappa B response to LPS
Yokoseki et al., Circ Res 2001 (Autoimmune Diseases...) : Nuclear factor-kappaB (NFkappaB) plays a significant role in the coordinated transactivation of cytokine, inducible NO synthase (iNOS), and adhesion molecule genes
Nicolini et al., J Neurochem 2001 : Our observations indicate that NF-kappaB plays a key role in Tat dependent microglial activation, and that oxidative stress and NF-kappaB activation induced by Tat occur by independent mechanisms
Liu et al., Mol Cell Biol Res Commun 2001 (MAP Kinase Signaling System) : While the Raf-1 activation induces a modest IkappaB degradation by enhancing the basal IkappaB kinase activity, it contradictorily suppresses the proinflammatory cytokine inducible IkappaB kinase complex, leading to an inhibition of TNF-alpha- and IL-1beta induced NF-kappaB activation
Hozumi et al., Am J Physiol Lung Cell Mol Physiol 2001 (Pneumonia) : In this study, we determined whether the proinflammatory cytokines tumor necrosis factor (TNF)-alpha and interleukin-1 beta contribute to the regulation of matrix metalloproteinase (MMP)-9 in human bronchial epithelial cells and whether the induction of MMP-9 is regulated by the transcription factor nuclear factor (NF)-kappa B ... Increased expression of MMP-9 and NF-kappa B activation induced by TNF-alpha were inhibited by pyrrolidine dithiocarbamate and N-acetyl-L-cysteine but were not inhibited by curcumin
Platten et al., Br J Pharmacol 2001 : 5. These results suggest that tranilast mediated suppression of microglial iNOS activity induced by IFN-gamma involves the inhibition of NF-kappaB dependent iNOS mRNA expression
Lam et al., Neurobiol Aging 2001 (Alzheimer Disease) : Mechanism of glial activation by S100B : involvement of the transcription factor NFkappaB
Mauriz et al., Free Radic Biol Med 2001 (Body Weight...) : Hemorrhagic shock resulted in oxidative stress ( significant elevations in TBARS and in the oxidized/reduced glutathione ratio ) and was accompanied by a reduced activity of the antioxidant enzymes Mn- and Cu, Zn-superoxide dismutase, glutathione peroxidase and catalase, overexpression of inducible NO synthase (iNOS) , and activation of nuclear factor kappa B (NF-kappaB) ... Glycine ameliorated oxidative stress and the impairment in antioxidant enzyme activities, inhibited NF-kappaB activation, and prevented expression of iNOS
Nanji et al., Am J Physiol Gastrointest Liver Physiol 2001 (Endotoxemia...) : We analyzed liver histopathology, lipid peroxidation, cytochrome P-450 (CYP)2E1 activity, nonheme iron, endotoxin, nuclear factor-kappa B (NF-kappa B) activation , and mRNA levels of cyclooxygenase-1 (COX-1) and COX-2, tumor necrosis factor-alpha (TNF-alpha) , monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein-2 ( MIP-2 ) ... We analyzed liver histopathology, lipid peroxidation, cytochrome P-450 (CYP)2E1 activity, nonheme iron, endotoxin, nuclear factor-kappa B (NF-kappa B) activation , and mRNA levels of cyclooxygenase-1 (COX-1) and COX-2, tumor necrosis factor-alpha (TNF-alpha), monocyte chemotactic protein-1 (MCP-1) , and macrophage inflammatory protein-2 ( MIP-2 ) ... We analyzed liver histopathology, lipid peroxidation, cytochrome P-450 (CYP)2E1 activity, nonheme iron, endotoxin, nuclear factor-kappa B (NF-kappa B) activation , and mRNA levels of cyclooxygenase-1 (COX-1) and COX-2, tumor necrosis factor-alpha (TNF-alpha), monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein-2 ( MIP-2 ) ... We analyzed liver histopathology, lipid peroxidation, cytochrome P-450 (CYP)2E1 activity, nonheme iron, endotoxin, nuclear factor-kappa B (NF-kappa B) activation , and mRNA levels of cyclooxygenase-1 (COX-1) and COX-2 , tumor necrosis factor-alpha (TNF-alpha), monocyte chemotactic protein-1 (MCP-1), and macrophage inflammatory protein-2 ( MIP-2 )
Hatano et al., Am J Physiol Gastrointest Liver Physiol 2001 : Active Akt induces NF-kappa B transcriptional activity but not NF-kappa B binding activity or I kappa B degradation
Ianaro et al., FEBS Lett 2001 (Disease Models, Animal...) : In conclusion, we demonstrate that cyclopentenone prostaglandins may act as anti-inflammatory mediators by inducing in inflammatory cells HSF1 dependent hsp72 expression and NF-kappaB inhibition, two crucial events for the remission of inflammation
Traincard et al., J Cell Sci 2001 : Addition and correction : the NF-kappa B-like DNA binding activity observed in Dictyostelium nuclear extracts is due to the GBF transcription factor
Vega et al., Adv Anat Pathol 2001 (Lymphoma, B-Cell, Marginal Zone...) : Recent work suggests that API2-MALT1 and BCL-10-MALT1 may activate NF-kB and a common downstream signaling pathway ... Recent work suggests that API2-MALT1 and BCL-10-MALT1 may activate NF-kB and a common downstream signaling pathway ... Recent work suggests that API2-MALT1 and BCL-10-MALT1 may activate NF-kB and a common downstream signaling pathway
Brady et al., Biochem Biophys Res Commun 2001 (Hypersensitivity) : This study suggests that p38 MAPK is crucially important in mediating CD40 activation of NF kappa B which acts to induce C epsilon germline transcripts, ultimately facilitating IgE switching
Zhang et al., J Virol 2001 : In addition, NF-kappaB is involved in the induction of IRF-7 ... A superrepressor of IkappaB ( sr-IkappaB ) could block the induction of IRF-7 by LMP-1, and overexpression of NF-kappaB ( p65 plus p50 ) could induce the expression of IRF-7
Ji et al., J Autoimmun 2001 (Arthritis, Rheumatoid...) : The effect of PPAR-gamma on the nuclear factor (NF)-kappaB activity was tested by electrophoretic mobility shift assay ( EMSA ) ... Both troglitazone and 15d-PGJ ( 2 ) markedly inhibited TNF-alpha induced NF-kappaB activation at 30 microM
Papouli et al., J Biol Chem 2002 : All these data suggest that the overexpression of MT-II in V-H4 cells impairs NF-kappaB activation by MMC, resulting in decreased cell survival and enhanced induction of apoptosis
Wooten et al., Mol Cell Biol 2001 : The functional relevance of these mutations was tested by determining the ability of each mutant to support TRAF6 activation of NF-kappaB , with significant impairment by the Y325F PKC-iota mutant
Yum et al., J Immunol 2001 (Endotoxemia...) : In the present study, we found that exposure of neutrophils to endotoxin resulted in phosphorylation of Akt , activation of NF-kappaB , and expression of the proinflammatory cytokines IL-1beta and TNF-alpha through PI3-K dependent pathways
Zhong et al., Zhonghua Yi Xue Za Zhi 1999 : To investigate whether TNF-alpha could activate the signaling pathway-NF-kappa B/I-kappa B alpha required for the expression of inducible nitric oxide synthase (iNOS) to induce endothelial cell apoptosis by nitric oxide ( NO )
López-Urrutia et al., Biochem Biophys Res Commun 2001 (Brucellosis...) : For this purpose, the induction of cyclooxygenase-2 (COX-2), the production of the chemokine monocyte chemoattractant protein-1 ( MCP-1 ), and the activation of the nuclear factor kappa B (NF-kappa B) were studied ... For this purpose, the induction of cyclooxygenase-2 (COX-2) , the production of the chemokine monocyte chemoattractant protein-1 ( MCP-1 ), and the activation of the nuclear factor kappa B (NF-kappa B) were studied
Zamorano et al., Int Immunol 2001 : IL-4 enhanced TNF-alpha induced activation of the transcription factor NF-kappaB ... IL-4 enhanced TNF-alpha induced activation of the transcription factor NF-kappaB ... Supporting this hypothesis, IL-4 also increased NF-kappaB activation induced by anti-CD3 antibodies in primary T cells and protected them from apoptosis induced by receptor engagement
Wei et al., J Biol Chem 2002 (MAP Kinase Signaling System) : We find that although pretreatment of BMMs with IL-4 does not alter M-CSF signaling, it reversibly blocks RANKL dependent activation of the NF-kappa B , JNK, p38, and ERK signals ... Consistent with this observation, the inhibitory effects of IL-4 on RANKL induced NF-kappa B and mitogen activated protein kinase activation are STAT6 dependent ... Consistent with this observation, the inhibitory effects of IL-4 on RANKL induced NF-kappa B and mitogen activated protein kinase activation are STAT6 dependent ... Consistent with this observation, the inhibitory effects of IL-4 on RANKL induced NF-kappa B and mitogen activated protein kinase activation are STAT6 dependent
Gründker et al., Eur J Endocrinol 2001 (Endometrial Neoplasms) : In addition, LHRH activates nucleus factor kappaB (NFkappaB) and protects the cancer cells from apoptosis
Tan et al., J Biol Chem 2002 : Overexpression of ILK stimulated NF-kappaB activity and inhibition of ILK or protein kinase B ( PKB/Akt ) suppressed this activation ... Overexpression of ILK stimulated NF-kappaB activity and inhibition of ILK or protein kinase B ( PKB/Akt ) suppressed this activation
Sakai et al., J Biochem 2001 : Receptor activator of NF-kappaB ( RANKL ) significantly increased the LBC levels of cathepsin K, TRAP, and c-Src , whereas calcitonin decreased the LBC levels of cathepsin K, TRAP, and Rab7, indicating that the transformation of early to late endosomal elements and lysosomes in OCLs is also regulated by osteoclastogenesis regulatory factors ... Receptor activator of NF-kappaB ( RANKL ) significantly increased the LBC levels of cathepsin K, TRAP , and c-Src, whereas calcitonin decreased the LBC levels of cathepsin K, TRAP, and Rab7, indicating that the transformation of early to late endosomal elements and lysosomes in OCLs is also regulated by osteoclastogenesis regulatory factors ... Receptor activator of NF-kappaB ( RANKL ) significantly increased the LBC levels of cathepsin K , TRAP, and c-Src, whereas calcitonin decreased the LBC levels of cathepsin K, TRAP, and Rab7, indicating that the transformation of early to late endosomal elements and lysosomes in OCLs is also regulated by osteoclastogenesis regulatory factors ... Receptor activator of NF-kappaB ( RANKL ) significantly increased the LBC levels of cathepsin K, TRAP, and c-Src, whereas calcitonin decreased the LBC levels of cathepsin K, TRAP, and Rab7, indicating that the transformation of early to late endosomal elements and lysosomes in OCLs is also regulated by osteoclastogenesis regulatory factors
Mansell et al., FEBS Lett 2001 : The serine protease inhibitor antithrombin III inhibits LPS mediated NF-kappaB activation by TLR-4
Wang et al., FEBS Lett 2001 : Adenoviral expression of a mutant form of the inhibitor of NF-kappaB, IkappaB-alphaM, suppressed TNF-alpha triggered degradation of cellular IkappaB-alpha , inhibited activation of NF-kappaB , and attenuated TNF-alpha induced migration
Zhou et al., Free Radic Biol Med 2001 : To test whether NF kappa B or AP-1 might be mediating the induction of GPx and CAT in muscle cells subjected to oxidative stress, we first characterized their activation by pro-oxidants ... To test whether NF kappa B or AP-1 might be mediating the induction of GPx and CAT in muscle cells subjected to oxidative stress, we first characterized their activation by pro-oxidants ... In summary, our results suggest that NF kappa B and AP-1 are important mediators of redox-responsive gene expression in skeletal muscle, and that at least NF kappa B is actively involved in the upregulation of the GPx and CAT in response to oxidative stress ... In summary, our results suggest that NF kappa B and AP-1 are important mediators of redox-responsive gene expression in skeletal muscle, and that at least NF kappa B is actively involved in the upregulation of the GPx and CAT in response to oxidative stress ... In summary, our results suggest that NF kappa B and AP-1 are important mediators of redox-responsive gene expression in skeletal muscle, and that at least NF kappa B is actively involved in the upregulation of the GPx and CAT in response to oxidative stress
Kim et al., FASEB J 2002 : Using human umbilical vein endothelial cells, we found that inhibitors of guanine-cytosine-rich DNA binding protein and nuclear factor (NF)-kB suppressed VEGF- and TNF-a induced expression and activity of TF
Fan et al., J Biol Chem 2002 : In this study, we explored a novel function of polymorphonuclear neutrophils ( PMN ) NAD ( P ) H oxidase in the mechanism of tumor necrosis factor-alpha (TNFalpha) induced NF-kappaB activation and intercellular adhesion molecule-1 ( ICAM-1 ) expression in endothelial cells ... Induction of neutropenia using anti-PMN serum prevented the initial TNFalpha induced NF-kappaB activation and ICAM-1 expression in WT mice, indicating the involvement of PMN NAD ( P ) H oxidase in signaling these responses ... Thus, oxidant signaling by the PMN NAD ( P ) H oxidase complex is an important determinant of TNFalpha induced NF-kappaB activation and ICAM-1 expression in endothelial cells
Kwon et al., Nitric Oxide 2001 : We found that both IL-1beta and TNF-alpha could independently activate cytosolic NF-kappaB , direct its translocation into the nucleus, and induce iNOS monomer synthesis ... We found that both IL-1beta and TNF-alpha could independently activate cytosolic NF-kappaB , direct its translocation into the nucleus, and induce iNOS monomer synthesis ... We found that both IL-1beta and TNF-alpha could independently activate cytosolic NF-kappaB , direct its translocation into the nucleus, and induce iNOS monomer synthesis
Sun et al., J Biol Chem 2002 : This interaction is a prerequisite for RIP3 mediated phosphorylation of RIP and subsequent attenuation of TNF induced NF-kappaB activation
Tago et al., Eur J Biochem 2001 : Presence of a genistein-responsive inhibitory mechanism on interleukin-1alpha induced NF-kappaB activation ... In the present study, we found that genistein, a tyrosine kinase inhibitor, augmented IL-1alpha dependent NF-kappaB activation, suggesting the presence of a tyrosine kinase mediating a suppression signal on NF-kappaB ... As determined by luciferase reporter gene assay using kappaB-responsive element, genistein enhanced IL-1alpha induced NF-kappaB activation
Liu et al., Br J Pharmacol 2001 : 7. These studies show that NFkappaB plays an important role in the regulation of IRF-1 induction in HUVECs
al-Ramadi et al., J Immunol 2001 : Moreover, the block is associated with a defective degradation of IkappaB-alpha and an incomplete activation of NF-kappaB heterodimeric complexes
Wang et al., J Immunol 2001 : NFkappaB and Sp1 elements are necessary for maximal transcription of toll-like receptor 2 induced by Mycobacterium avium
Liu et al., J Leukoc Biol 2001 (Gout) : PP1 inhibited crystal induced phosphorylation of ERK1/2 and IkappaBalpha and suppressed IkappaB kinase (IKK) activation and NF-kappaB binding to the IL-8 promoter, signals that mediate MSU induced IL-8 expression
Hideshima et al., Semin Oncol 2001 (Multiple Myeloma...) : PS-341 blocks the paracrine growth of human MM cells by decreasing their adherence to BMSCs and related NF-kappaB dependent induction of IL-6 secretion in BMSCs ... Tumor necrosis factor-alpha (TNF-alpha), which is produced by some MM cells, induces only low-level MM proliferation and MAPK activation in MM cells, but markedly upregulates IL-6 secretion from BMSCs and upregulates expression of adhesion molecules ( VLA-4 and LFA-1 ) on MM cells and their receptors ( VCAM-1 and ICAM-1 ) on BMSCs, with resultant increased binding of MM cells to BMSCs. Inhibition of TNF-alpha induced NF-kappaB activation with PS-341 inhibits both the upregulation of these molecules on MM cells and BMSCs and the resultant increased adhesion
Adams et al., Semin Oncol 2001 (Hematologic Neoplasms...) : Myeloma cells depend on NF-kappaB mediated transcription of cytokine growth factor interleukin-6 , angiogenesis through vascular endothelial growth factor, and the cell adhesion molecule VCAM-1 for adherence of the plasma cells to the stromal tissue in bone marrow
Berenson et al., Semin Oncol 2001 (Multiple Myeloma) : Release of activated NF-kappaB follows proteasome mediated degradation of IkappaBalpha resulting from phosphorylation of the inhibitor and finally conjugation with ubiquitin
Kizaki et al., Biochem Biophys Res Commun 2001 : Although no significant difference was observed in the activation of NF-kappaB by LPS among parental J774A.1, J774A.1 transfected with promoterless vector ( J.pBK ), and J.Csk cells, activity of c-Jun N-terminal kinase (JNK) and nuclear translocation of nuclear factor activator protein-1 (AP-1) were markedly upregulated in the J.Csk cells
Nakamura et al., J Biol Chem 2002 (MAP Kinase Signaling System) : Analyzing the effects of several inhibitors or mutant receptors revealed that this NF-kappa B activation is mediated neither by MEK/ERK/MAPK nor by the phosphatidylinositol 3-kinase pathway but by STAT5 ... Analyzing the effects of several inhibitors or mutant receptors revealed that this NF-kappa B activation is mediated neither by MEK/ERK/MAPK nor by the phosphatidylinositol 3-kinase pathway but by STAT5 ... Analyzing the effects of several inhibitors or mutant receptors revealed that this NF-kappa B activation is mediated neither by MEK/ERK/MAPK nor by the phosphatidylinositol 3-kinase pathway but by STAT5 ... In addition, STAT5 dependent NF-kappa B activation is mediated not through an inducible nuclear translocation but via up-regulation of both DNA binding activity and transactivation potential of NF-kappa B ... In addition, STAT5 dependent NF-kappa B activation is mediated not through an inducible nuclear translocation but via up-regulation of both DNA binding activity and transactivation potential of NF-kappa B
Polekhina et al., Nat Struct Biol 2002 : We find that the Siah1a SBD potentiates TNF-alpha mediated NF-kappa B activation
Teng et al., Am J Physiol Cell Physiol 2002 : IL-1 beta, but not IFN-gamma , induced nuclear factor (NF)-kappa B and CCAAT box/enhancer binding protein ( C/EBP ) nuclear binding ... IL-1 beta , but not IFN-gamma, induced nuclear factor (NF)-kappa B and CCAAT box/enhancer binding protein ( C/EBP ) nuclear binding ... We conclude that in RASMC, NF-kappa B and C/EBP mediate the IL-1 beta induced iNOS expression, whereas IRF-1 and STAT1 mediate the IFN-gamma enhanced iNOS induction
Jiang et al., Arterioscler Thromb Vasc Biol 2001 : IL-1beta induced a rapid and transient activation of nuclear factor-kappaB (NF-kappaB) , followed by a prolonged activation of NF-kappaB that was required to induce iNOS expression ... These data suggest that ERK activity is required for persistent NF-kappaB activation by IL-1beta that is necessary for iNOS gene expression ... These data suggest that ERK activity is required for persistent NF-kappaB activation by IL-1beta that is necessary for iNOS gene expression
Wald et al., Eur J Immunol 2001 : In this line which has low or no expression of the other known IRAK family members, we find that the IL-1 receptor associated kinase (IRAK) is essential for the activation of NFkappaB and JNK in response to IL-18 ... In this line which has low or no expression of the other known IRAK family members, we find that the IL-1 receptor associated kinase (IRAK) is essential for the activation of NFkappaB and JNK in response to IL-18 ... Furthermore, the death domain, but not the kinase activity of IRAK , is necessary for NFkappaB activation in response to IL-18 ... Furthermore, the death domain, but not the kinase activity of IRAK, is necessary for NFkappaB activation in response to IL-18 ... A dominant negative mutant of TAK1 inhibits the IL-18 mediated NFkappaB activation, while IL-18 stimulation leads to the phosphorylation of TAB1
Loukinova et al., Int J Cancer 2001 (Carcinoma, Squamous Cell...) : The enhanced expression of Gro-1 gene by SCC is regulated by activation of nuclear factor-kappaB (NF-kappaB) , leading to accelerated tumor growth, angiogenesis and metastasis in vivo ... In our study, we investigated the effect of the regulatory cytokines, IL-1alpha, EGF and TGF-beta1 on activation of NF-kappaB and Gro1 in primary and metastatic sublines of the murine SCC Pam 212 ... In our study, we investigated the effect of the regulatory cytokines, IL-1alpha, EGF and TGF-beta1 on activation of NF-kappaB and Gro1 in primary and metastatic sublines of the murine SCC Pam 212 ... In our study, we investigated the effect of the regulatory cytokines, IL-1alpha , EGF and TGF-beta1 on activation of NF-kappaB and Gro1 in primary and metastatic sublines of the murine SCC Pam 212
Dunn et al., Glia 2002 (Brain Ischemia) : Analysis of the effects of IL-1 on nuclear translocation of the transcription factor NF-kB revealed that NF-kB became activated in a time dependent manner ... Of particular interest is the finding that IL-1 stimulated activation of p38 MAP kinase and NF-kB in striatal astrocytes exclusively
Liu et al., Acta Pharmacol Sin 2001 (Brain Edema...) : Tet inhibited neutrophilic recruitment, expression of ICAM-1 mRNA, and activation of NF-kappaB after brain I/R
Kang et al., Am J Respir Crit Care Med 2001 (Respiratory Distress Syndrome, Adult) : A 2-h pretreatment with genistein ( 50 mg/kg, intraperitoneally ) inhibited the LPS induced changes in lung injury parameters and the induction of NF-kappaB activation
Haddad et al., Am J Respir Cell Mol Biol 2002 : Recombinant IL-10 blockaded LPS induced biosynthesis of IL-1beta and TNF-alpha and reduced NF-kappaB activation
Sasaki et al., Clin Cancer Res 2001 (Lymphatic Metastasis...) : There was a significant correlation between NF-kappaB activation ( nuclear translocation of RelA ) and expression of urokinase-type plasminogen activator, an invasion related factor and target of NF-kappaB in tumor cells ( rho = 0.393 ; P = 0.0013 )
Muenzner et al., J Biol Chem 2002 : N. gonorrhoeae triggered CEACAM1 up-regulation involves activation of the transcription factor nuclear factor kappaB (NF-kappaB) , which translocates as a p50/p65 heterodimer into the nucleus, and an NF-kappaB-specific inhibitory peptide inhibited CEACAM1-receptor up-regulation in N. gonorrhoeae infected HOSE cells
Tone et al., J Biol Chem 2002 : The role of Sp1 and NF-kappa B in regulating CD40 gene expression ... We also show here that CD40 expression in lipopolysaccharide stimulated cells is up-regulated by NF-kappaB through two distinct sites
Shin et al., J Biol Chem 2002 : A novel human C ( 2 ) H ( 2 ) -type zinc finger family protein was identified, which when coexpressed with TRAF6 led to a suppression of TRAF6 induced activation of NF-kappa B and c-Jun N-terminal kinase
Krisanaprakornkit et al., J Immunol 2002 (MAP Kinase Signaling System) : The role of NF-kappaB in hBD-2 regulation was investigated initially due to its importance in inflammation and infection ... Nuclear translocation of p65 and NF-kappaB activation was seen in human gingival epithelial cells stimulated with F. nucleatum cell wall extract, indicating possible involvement of NF-kappaB in hBD-2 regulation ... Our results suggest that NF-kappaB is neither essential nor sufficient for hBD-2 induction, and that hBD-2 regulation by F. nucleatum is via p38 and JNK, while phorbol ester induces hBD-2 via the p44/42 extracellular signal regulated kinase pathway
Romagnani et al., J Am Soc Nephrol 2002 (Kidney Diseases) : Electrophoretic mobility shift assays demonstrated that NO donors repressed IP-10 gene transcription induced by IFN-gamma plus TNF-alpha through the inhibition of NF-kappaB activation ... Electrophoretic mobility shift assays demonstrated that NO donors repressed IP-10 gene transcription induced by IFN-gamma plus TNF-alpha through the inhibition of NF-kappaB activation
Greiber et al., J Am Soc Nephrol 2002 : Activation of the transcription factor nuclear factor-kappaB (NF-kappaB) but not activator protein-1 was involved in the upregulation of ROS induced GM-CSF production
Courtois et al., Science's STKE : signal transduction knowledge environment 2000 (Incontinentia Pigmenti) : Courtois and Israël discuss the role of NEMO/IKKgamma in normal physiological activation of NF-kappaB and the consequences of defective NF-kappaB activation, as an effect of NEMO/IKKgamma mutations, which can lead to incontinentia pigmenti, a disease marked by alopecia, tooth eruption, skin lesions, and changes in skin pigmentation
D'Acquisto et al., Science's STKE : signal transduction knowledge environment 2001 : The activation of the transcription factor nuclear factor kappa B (NF-kappaB) by PKR has been shown to account for the host antiviral response ... We summarize the most recent findings on the molecular mechanisms leading to the activation of NF-kappaB by PKR and discuss three major unanswered questions
Williams et al., Science's STKE : signal transduction knowledge environment 2001 : This review will focus on the role of PKR in nuclear factor kappa B (NF-kappaB) and mitogen activated protein kinase ( MAPK ) pathways, because these have been the subjects of a series of publications over the past year that have reported conflicting findings
Mukhopadhyay et al., Oncogene 2001 (Prostatic Neoplasms) : TNF induced NF-kappaB activation but not AP-1 activation in LNCaP cells
Bren et al., Oncogene 2001 : Transcription of the RelB gene is regulated by NF-kappaB
Kasof et al., Oncogene 2001 (Prostatic Neoplasms) : Tumor necrosis factor-alpha induces the expression of DR6, a member of the TNF receptor family, through activation of NF-kappaB ... Analysis of several anti-apoptotic proteins revealed that Bcl-x ( L ) levels and serine 32 phosphorylation of IkappaB, the natural inhibitor of NF-kappaB , were similarly elevated in cells expressing high levels of DR6, suggesting that NF-kappaB regulated survival proteins may protect from DR6 induced apoptosis and that DR6 is a target of NF-kappaB regulation
Hotta et al., Eur J Pharmacol 2001 : In circulating blood neutrophils, TNF-alpha induced activation of nuclear factor-kappa B (NF-kappa B) , whereas, in tissue neutrophils, NF-kappa B had been already activated without any stimulation, and no further activation was induced by the treatment with TNF-alpha
Doi et al., Atherosclerosis 2002 : Both IL-1beta and TNF-alpha stimulated NF-kappaB activity, iNOS mRNA and protein expression with massive nitrite/nitrate ( NOx ) production in rat VSMCs. PTK inhibitors ( genistein, herbimycin A ) dose-dependently inhibited the cytokine stimulated NOx production and iNOS mRNA expression ... Both IL-1beta and TNF-alpha stimulated NF-kappaB activity, iNOS mRNA and protein expression with massive nitrite/nitrate ( NOx ) production in rat VSMCs. PTK inhibitors ( genistein, herbimycin A ) dose-dependently inhibited the cytokine stimulated NOx production and iNOS mRNA expression ... However, neither genistein nor herbimycin A affected the cytokine stimulated phosphorylation and degradation of IkappaB-alpha , or NF-kappaB activation , whereas they completely blocked the cytokine stimulated iNOS transcriptional activity
Park et al., Eur Respir J 2001 : Therefore, the blocking NF-kappaB activation by IkappaBalpha could inhibit the pro-inflammatory cytokine induced tissue injury
Li et al., Zhonghua Bing Li Xue Za Zhi 2001 : In this project the effect of angiotensin II (Ang II) on NF-kappa B activation and the promotion of PDGF-B mRNA expression in human endothelial cell line ECV304 was studied ... Electrophoretic mobility shift assay, immunofluorescence and immunoelectronic microscope techniques, including confocal microscopy and gold particle labelled electronic microscopy were applied to investigate the mechanism by which Ang II activates NF-kappa B , ECV304 cells were transiently transfected with an NF-kappa B/luciferase reporter gene and catalytically inactive NIK, IKK alpha, IKK beta mutants respectively ... Ang II is effective to activate NF-kappa B through a pathway dependent on NIK, IKK alpha and IKK beta, and induces PDGF-B transcription in the endothelial cell line ECV304
Kohno et al., J Rheumatol 2001 : Expression of interleukin 6 (IL-6) and IL-8 mRNA was examined by real-time quantitative reverse transcriptase-polymerase chain reaction analysis and TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation was observed using immunostaining with an antibody against NF-kappaB p65
Nomura et al., Yakugaku Zasshi 2001 : Regarding the results of studies on the inter- and intra-cellular mechanism of neurons and glial cells against cerebral ischemia/hypoxia, we review the involvement of a transcription factor NF-kappa B in LPS elicited inducible NO synthase (iNOS) expression in rat astroglial cells
Blanchard et al., Dig Dis Sci 2001 (Adenocarcinoma...) : Previously, we have demonstrated that tumor necrosis factor-alpha (TNF-alpha) and gram negative bacterial lipopolysaccharide stimulate production of IL-6 and IL-8 and activation of the transcription factor NF-kappaB in the well differentiated pancreatic ductal adenocarcinoma cell lines CAPAN-1 and CAPAN-2 ... Previously, we have demonstrated that tumor necrosis factor-alpha (TNF-alpha) and gram negative bacterial lipopolysaccharide stimulate production of IL-6 and IL-8 and activation of the transcription factor NF-kappaB in the well differentiated pancreatic ductal adenocarcinoma cell lines CAPAN-1 and CAPAN-2 ... Previously, we have demonstrated that tumor necrosis factor-alpha (TNF-alpha) and gram negative bacterial lipopolysaccharide stimulate production of IL-6 and IL-8 and activation of the transcription factor NF-kappaB in the well differentiated pancreatic ductal adenocarcinoma cell lines CAPAN-1 and CAPAN-2
Simpson et al., Expert Opin Investig Drugs 2001 (Graft vs Host Disease) : NF-kappa B is also required for CD28 ligation signalling, important in late co-stimulation
Mitsiades et al., Expert Opin Investig Drugs 2001 (Multiple Myeloma...) : Moreover, NF-kappaB inhibitors such as SN50 ( a cell permeable inhibitor of NF-kappaB nuclear translocation ) as well as the proteasome inhibitor PS-341, which is currently in Phase II clinical trials, also enhanced the pro-apoptotic activity of TRAIL/Apo2L in MM cells
Kanekiyo et al., Biochem J 2002 : This conclusion is supported by the following observations : ( 1 ) LPS stimulated the secretion of less TNF activity from MT ( -/- ) peritoneal exudate macrophages ( PEMs ) than from wild-type controls ( MT ( +/+ ) mice ) without a difference in the pattern of kinetics ; ( 2 ) LPS stimulated expression of TNF-alpha mRNA was decreased in MT ( -/- ) PEMs ; ( 3 ) LPS stimulated activation of NF-kappaB was decreased in MT ( -/- ) PEMs ; and ( 4 ) production of TNF in PEMs of MT ( -/- ) mice after LPS treatment in vivo was decreased ( compared with MT ( +/+ ) PEMs )
Dhawan et al., J Biol Chem 2002 (MAP Kinase Signaling System...) : This increase in I kappa B-alpha phosphorylation and degradation leads to an approximately 19-fold higher nuclear localization of NF-kappa B ... Moreover, the NF-kappa B promoter activity decreased with overexpression of dominant negative ERK expression constructs, and EMSA analyses further support the hypothesis that ERK acts upstream of NF-kappa B and regulates the NF-kappa B DNA binding activity ... Taken together, our data implicate involvement of I kappa B kinase and MAPK signaling cascades in NIK induced constitutive activation of NF-kappa B ... Taken together, our data implicate involvement of I kappa B kinase and MAPK signaling cascades in NIK induced constitutive activation of NF-kappa B
Vancurova et al., J Virol 2002 (Laryngeal Neoplasms...) : Increased NF-kappaB activity and cytoplasmic accumulation of p21 ( CIP1/WAF1 ) might counteract death promoting effects elicited by overexpressed PTEN and reduced activation of Akt and STAT3 previously noted in these tissues ... Increased NF-kappaB activity and cytoplasmic accumulation of p21 ( CIP1/WAF1 ) might counteract death promoting effects elicited by overexpressed PTEN and reduced activation of Akt and STAT3 previously noted in these tissues
Segev et al., Proc Natl Acad Sci U S A 2002 : MIS induced NF-kappaB DNA binding activity and selectively up-regulated the immediate early gene IEX-1S in both androgen dependent and independent human prostate cancer cells in vitro
Sanlioglu et al., Cancer Gene Ther 2001 (Lung Neoplasms) : A549 human lung carcinoma cells were infected with adenoviral constructs carrying dominant negative mutants of Rac1 and IKK or constitutively active mutant of Rac1, upstream effectors in TNF mediated NF-kappaB activation
Maliner-Stratton et al., Neoplasia (New York, N.Y.) 2001 (Adenocarcinoma...) : Interleukin-1beta induced promatrilysin expression is mediated by NFkappaB regulated synthesis of interleukin-6 in the prostate carcinoma cell line, LNCaP ... We now demonstrate that IL-1beta induced promatrilysin expression is mediated by an indirect mechanism that requires nuclear factor Kappa B (NFkappaB) dependent synthesis of IL-6 ... Inhibition of IL-6 activity attenuated IL-1beta induced promatrilysin, but not NFkappaB transactivation activity indicating that IL-6 acts downstream of NFkappaB in potentiation of IL-1beta mediated promatrilysin expression ... Inhibition of IL-6 activity attenuated IL-1beta induced promatrilysin, but not NFkappaB transactivation activity indicating that IL-6 acts downstream of NFkappaB in potentiation of IL-1beta mediated promatrilysin expression ... These data provide evidence that NFkappaB mediated IL-6 synthesis is required for IL-1beta induced promatrilysin expression, and IL-6 signaling through STAT3 plays a role in IL-1beta induced promatrilysin expression
Cai et al., Zhonghua Jie He He Hu Xi Za Zhi 1999 : LPS might activate NF-kappa B in the PIMs, and induce the increase of transcription and expression of TNF-alpha gene ; Both DEX and ASA could inhibit the activation of NF-kappa B and reduce the release of TNF-alpha ... LPS might activate NF-kappa B in the PIMs, and induce the increase of transcription and expression of TNF-alpha gene ; Both DEX and ASA could inhibit the activation of NF-kappa B and reduce the release of TNF-alpha ... LPS might activate NF-kappa B in the PIMs, and induce the increase of transcription and expression of TNF-alpha gene ; Both DEX and ASA could inhibit the activation of NF-kappa B and reduce the release of TNF-alpha
Bannerman et al., J Biol Chem 2002 : Although the mechanism by which LPS activates NF-kappa B has been well elucidated, the signaling pathway ( s ) involved in LPS induced apoptosis remains unknown ... We also demonstrate that LPS induced endothelial NF-kappa B activation and apoptosis occur independent of one another ... Together, these data suggest that the proximal signaling molecules involved in LPS induced NF-kappa B activation have a requisite involvement in LPS induced apoptosis and that the pathways leading to NF-kappa B activation and apoptosis diverge downstream of IRAK-1 ... Together, these data suggest that the proximal signaling molecules involved in LPS induced NF-kappa B activation have a requisite involvement in LPS induced apoptosis and that the pathways leading to NF-kappa B activation and apoptosis diverge downstream of IRAK-1
Xia et al., J Biol Chem 2002 : We identified a TRAF2 binding motif of SphK that mediated the interaction between TRAF2 and SphK resulting in the activation of the enzyme, which in turn is required for TRAF2 mediated activation of NF-kappa B but not JNK
Shimada et al., J Immunol 2002 : EMSAs demonstrated that IL-17, IL-1beta, and TNF-alpha induced NF-kappaB activation within 1.5 h after stimulation, and a blockade of NF-kappaB activation by the pyrrolidine derivative of dithiocarbamate and tosyl-phe-chloromethylketone markedly reduced the IL-17-, IL-1beta-, or TNF-alpha induced IL-6 gene expression ... EMSAs demonstrated that IL-17 , IL-1beta, and TNF-alpha induced NF-kappaB activation within 1.5 h after stimulation, and a blockade of NF-kappaB activation by the pyrrolidine derivative of dithiocarbamate and tosyl-phe-chloromethylketone markedly reduced the IL-17-, IL-1beta-, or TNF-alpha induced IL-6 gene expression ... EMSAs demonstrated that IL-17, IL-1beta , and TNF-alpha induced NF-kappaB activation within 1.5 h after stimulation, and a blockade of NF-kappaB activation by the pyrrolidine derivative of dithiocarbamate and tosyl-phe-chloromethylketone markedly reduced the IL-17-, IL-1beta-, or TNF-alpha induced IL-6 gene expression
Narayanan et al., Cell Biol Int 2002 : CD14 dependent activation of NF-kappaB by filarial parasitic sheath proteins ... Herein we provide evidence that FPS activation of NF-kappaB can be augmented by the cell surface expression of CD14 ... Herein we provide evidence that FPS activation of NF-kappaB can be augmented by the cell surface expression of CD14 ... These observations suggest that the capacity of certain lung epithelial cells to interact with microfilarial antigens, activate NF-kappaB in a CD14 dependent manner and produce pro-inflammatory cytokines may be a contributory factor to immune responses manifested by tropical pulmonary eosinophilia
Yin et al., Chin Med J (Engl) 2001 (Nasopharyngeal Neoplasms) : LMP1 activates NF-kappa B via degradation of I kappa B alpha in nasopharyngeal carcinoma cells ... After the introduction of the dominant negative mutant of I kappa B alpha ( Del 71 ) into Tet-on-LMP1-HNE2 cells, both nuclear translocation and transactivation of NF-kappa B induced by LMP1 was significantly inhibited ... The results indicated that in nasopharyngeal carcinoma cells, LMP1 activated NF-kappa B via phosphorylation and degradation of I kappa B alpha, but not I kappa B beta ... The dominant negative mutant of I kappa B alpha ( Del 71 ) could completely inhibit both the nuclear translocation and transactivation of NF-kappa B induced by LMP1
Beppu et al., Biochim Biophys Acta 2002 (Carcinoma, Squamous Cell...) : Regarding their mechanisms to inhibit the expression of MMP-9 and uPA, DEX and IL-4 had no effect on the cell surface levels of TNFalpha receptors, but inhibited the activation of NF-kappaB and NF-kappaB dependent gene expression
Haddad et al., Eur Cytokine Netw 2001 : The regulation of cytokines involves the reduction-oxidation ( redox ) -sensitive nuclear factor-kappaB (NF-kappaB), the activation of which is mediated through an upstream kinase that regulates the phosphorylation and subsequent degradation of inhibitory-kappaB (IkappaB)-alpha , the major cytosolic inhibitor of NF-kappaB ... Interestingly, BSO blocked the phosphorylation of IkappaB-alpha , reduced its degradation, thereby allowing its cytosolic accumulation, and subsequently inhibited the activation of NF-kappaB
Brand et al., Gastroenterology 2002 (MAP Kinase Signaling System...) : Fractalkine activated NF-kappa B and p53 resulting in interleukin 8 and fractalkine mRNA expression
Tsutsumi-Ishii et al., J Leukoc Biol 2002 : NF-kappa B-mediated transcriptional regulation of human beta-defensin-2 gene following lipopolysaccharide stimulation ... Together, these observations indicate that NF-kappa B plays an important role in the regulation of hBD-2 gene expression in response to LPS
Vockerodt et al., Genes Immun 2001 (Burkitt Lymphoma...) : Inhibition of LMP1 mediated NFkappaB enhancement by a constitutive repressive form of IkappaB-alpha resulted in decreased CD25 surface expression, indicating that NFkappaB is involved in CD25 gene regulation
Thomas et al., Immunol Rev 2001 : DSG , an inhibitor of HSC 70-mediated NF-kappaB nuclear translocation, arrests maturation of myeloid dendritic cells, blocks production of proinflammatory cytokines induced by IT administration, and promotes systemic production of Th2 type cytokines that persist indefinitely
Liao et al., Zhonghua Zhong Liu Za Zhi 2001 (Nasopharyngeal Neoplasms) : Induction of p53 expression could be blocked by phosphorothiate analogs of antisense oligonucleotides to NF-kappa B p65 and LMP1, but not by NF-kappa B p50
Choi et al., Mol Cell Biol 2002 (Astrocytoma...) : These findings collectively indicate that DR5 ligation on human glioma cells leads to apoptosis and that the activation of AP-1 and NF-kappaB leads to the induction of IL-8 expression ; these responses are dependent on caspase activation
Cahir-McFarland et al., Recent Results Cancer Res 2002 (Cell Transformation, Viral) : NF-kappaB inhibition caused caspase 3 and 8 activation, PARP cleavage, and DNA fragmentation indicative of apoptosis
Manji et al., J Biol Chem 2002 : When expressed in cells, ASC recruits PYPAF1 to distinct cytoplasmic loci and induces the activation of NF-kappaB ... Furthermore, coexpression of PYPAF1 with ASC results in a potent synergistic activation of NF-kappaB ... These findings suggest that PYPAF1 and ASC function as upstream activators of NF-kappaB signaling
Cho et al., Int Immunopharmacol 2002 : The effects of arctigenin and demethyltraxillagenin on the nuclear factor-kappaB (NF-kappaB) mediated inducible nitric oxide synthase ( iNOS, EC1.14.13.39 ) gene expression were studied in Raw264.7 cells
Cabrero et al., J Biol Chem 2002 : These results indicate that ROS generation and NF-kappaB activation are responsible for the down-regulation of PPARalpha and may provide a new mechanism by which intracellular lipid accumulation occurs in skeletal muscle cells
Mansky et al., J Biol Chem 2002 (MAP Kinase Signaling System) : Microphthalmia transcription factor is a target of the p38 MAPK pathway in response to receptor activator of NF-kappa B ligand signaling
Pandey et al., Endocrinology 2002 : In contrast, cell stimulation with the cytokine TNFalpha allowed activation of NFkappaB through phosphorylation, ubiquitination, and subsequent degradation of IkappaBalpha ... In contrast, cell stimulation with the cytokine TNFalpha allowed activation of NFkappaB through phosphorylation, ubiquitination, and subsequent degradation of IkappaBalpha
Galdiero et al., Infect Immun 2002 (MAP Kinase Signaling System) : PD-098059 pretreatment of cells decreases AP-1 and NF-kappaB activation by lipopolysaccharide (LPS) but not by porins, and SB203580 pretreatment of cells decreases mainly AP-1 and NF-kappaB activation by porins ; in contrast, forskolin pretreatment of cells does not affect AP-1 and NF-kappaB activation following either porin or LPS stimulation
Jana et al., J Neurochem 2002 : However, CD40 ligation alone in the presence or absence of CD40 overexpression induced the activation of only NF-kappaB and not that of C/EBPbeta, suggesting that the activation of NF-kappaB alone by CD40 ligation is not sufficient to induce the expression of TNF-alpha and that the activation of C/EBPbeta is also necessary for strong induction of TNF-alpha ... However, CD40 ligation alone in the presence or absence of CD40 overexpression induced the activation of only NF-kappaB and not that of C/EBPbeta, suggesting that the activation of NF-kappaB alone by CD40 ligation is not sufficient to induce the expression of TNF-alpha and that the activation of C/EBPbeta is also necessary for strong induction of TNF-alpha ... Taken together, these studies suggest that activation of both NF-kappaB and C/EBPbeta is important for strong induction of TNF-alpha and that CD40 ligation regulates the expression of TNF-alpha by modulating the activation of only NF-kappaB but not that of C/EBPbeta ... Taken together, these studies suggest that activation of both NF-kappaB and C/EBPbeta is important for strong induction of TNF-alpha and that CD40 ligation regulates the expression of TNF-alpha by modulating the activation of only NF-kappaB but not that of C/EBPbeta ... Taken together, these studies suggest that activation of both NF-kappaB and C/EBPbeta is important for strong induction of TNF-alpha and that CD40 ligation regulates the expression of TNF-alpha by modulating the activation of only NF-kappaB but not that of C/EBPbeta
Kang et al., Biochem Biophys Res Commun 2002 : Increased expression of iNOS and activation of NFkappaB by VVC were confirmed by Western blotting and gel shift assay, respectively
Mayo et al., J Biol Chem 2002 : PTEN blocks tumor necrosis factor induced NF-kappa B-dependent transcription by inhibiting the transactivation potential of the p65 subunit ... PTEN blocks tumor necrosis factor induced NF-kappa B-dependent transcription by inhibiting the transactivation potential of the p65 subunit ... Although the Akt pathway has been implicated in regulating NF-kappaB activity, it is controversial as to whether Akt activates NF-kappaB predominantly through mechanisms that regulate nuclear translocation or transactivation potential ... In this report, we utilized PTEN as a natural biological inhibitor of Akt activity to study the effects on tumor necrosis factor (TNF) induced activation of NF-kappaB ... We found that the reintroduction of PTEN into prostate cells inhibited TNF stimulated NF-kappaB transcriptional activity ... PTEN failed to block TNF induced IKK activation , IkappaBalpha degradation, p105 processing, p65 ( RelA ) nuclear translocation, and DNA binding of NF-kappaB ... PTEN failed to block TNF induced IKK activation , IkappaBalpha degradation, p105 processing, p65 ( RelA ) nuclear translocation, and DNA binding of NF-kappaB ... Maintenance of the PTEN tumor suppressor protein is therefore required to modulate Akt activity and to concomitantly control the transcriptional activity of the anti-apoptotic transcription factor NF-kappaB
Bulut et al., J Immunol 2002 : In 293 cells, cHSP60 mediated NF-kappaB activation required both TLR4 and MD2
Gasparian et al., J Cell Sci 2002 (Carcinoma...) : The role of IKK in constitutive activation of NF-kappaB transcription factor in prostate carcinoma cells
Seo et al., Pharmacology 2002 : Small-molecule antioxidants and SOD inhibited NF-kappaB activation and cytokine production in acinar cells caused by PMA primed neutrophils
Jang et al., Mol Cells 2001 : Overexpression of LRR-1 suppressed the activation of NF-KB induced by 4-1BB or TNF receptor associated factor (TRAF) 2 ... Overexpression of LRR-1 suppressed the activation of NF-KB induced by 4-1BB or TNF receptor associated factor (TRAF) 2 ... Overexpression of LRR-1 suppressed the activation of NF-KB induced by 4-1BB or TNF receptor associated factor (TRAF) 2
Haddad et al., J Pharmacol Exp Ther 2002 (Inflammation...) : In fetal alveolar type II epithelial cells, PDE blockade at the level of the diverging cAMP/cGMP pathways differentially regulated the phosphorylation and degradation of IkappaB-alpha , the major cytosolic inhibitor of NF-kappaB ... Whereas selective inhibition of PDEs 1, 3, and 4, by the action of 8-methoxymethyl-3-isobutyl-1-methylxanthine, amrinone, and rolipram, respectively, exhibited a tendency to augment the translocation of NF-kappaB ( 1 ) ( p50 ), RelA ( p65 ), RelB ( p68 ), and c-Rel ( p75 ), selective blockade of PDE 5, 6, and 9, by the action of 4- [ [ 3',4'- ( methylenedioxy ) benzyl ] amino ] -6-methoxyquinazoline and zaprinast, attenuated lipopolysaccharide-endotoxin (LPS) mediated NF-kappaB translocation ... Whereas selective inhibition of PDEs 1, 3, and 4, by the action of 8-methoxymethyl-3-isobutyl-1-methylxanthine, amrinone, and rolipram, respectively, exhibited a tendency to augment the translocation of NF-kappaB ( 1 ) ( p50 ), RelA ( p65 ), RelB ( p68 ), and c-Rel ( p75 ), selective blockade of PDE 5, 6, and 9, by the action of 4- [ [ 3',4'- ( methylenedioxy ) benzyl ] amino ] -6-methoxyquinazoline and zaprinast, attenuated lipopolysaccharide-endotoxin (LPS) mediated NF-kappaB translocation ... In contrast, blockading the activity of PDEs 5, 6, and 9 negatively attenuated LPS mediated NF-kappaB activation, similar to the effect of 3,7-dihydro-3,7-dimethyl-1- ( 5-oxohexyl ) -1H-purine-2,6-dione ( pentoxifylline ) ... Whereas selective inhibition of PDEs 1, 3, and 4, by the action of 8-methoxymethyl-3-isobutyl-1-methylxanthine, amrinone, and rolipram, respectively, exhibited a tendency to augment the translocation of NF-kappaB ( 1 ) ( p50 ), RelA ( p65 ), RelB ( p68 ), and c-Rel ( p75 ), selective blockade of PDE 5, 6, and 9, by the action of 4- [ [ 3',4'- ( methylenedioxy ) benzyl ] amino ] -6-methoxyquinazoline and zaprinast, attenuated lipopolysaccharide-endotoxin (LPS) mediated NF-kappaB translocation ... Whereas selective inhibition of PDEs 1, 3, and 4, by the action of 8-methoxymethyl-3-isobutyl-1-methylxanthine, amrinone, and rolipram, respectively, exhibited a tendency to augment the translocation of NF-kappaB ( 1 ) ( p50 ), RelA ( p65 ), RelB ( p68 ), and c-Rel ( p75 ), selective blockade of PDE 5, 6, and 9, by the action of 4- [ [ 3',4'- ( methylenedioxy ) benzyl ] amino ] -6-methoxyquinazoline and zaprinast, attenuated lipopolysaccharide-endotoxin (LPS) mediated NF-kappaB translocation
Tomita et al., Phytother Res 2002 : Specifically, we tested the hypothesis that cytokines produced by type 1 ( T ( H1 ) ) CD4 ( + ) T cells which require NF-kappaB for gene expression, such as interleukin-2 (IL-2) and interferon gamma (IFN gamma), are selectively inhibited by tea pigments ... We found that tea pigments potently suppress IL-2 secretion, IL-2 gene expression and the activation of NF-kappaB in murine spleens enriched for CD4 ( + ) T cells, as expected
Mizukami et al., Mol Cell Biol 2002 (MAP Kinase Signaling System) : Dominant negative forms of TAK1 and TAB2 inhibit NF-kappaB activation induced by overexpression of RANK ... Dominant negative forms of TAK1 and TAB2 inhibit NF-kappaB activation induced by overexpression of RANK ... Furthermore, in murine monocyte RAW 264.7 cells, dominant negative forms of TAK1 and TAB2 inhibit NF-kappaB activation induced by RANKL and endogenous TAK1 is activated in response to RANKL stimulation ... Furthermore, in murine monocyte RAW 264.7 cells, dominant negative forms of TAK1 and TAB2 inhibit NF-kappaB activation induced by RANKL and endogenous TAK1 is activated in response to RANKL stimulation ... Furthermore, in murine monocyte RAW 264.7 cells, dominant negative forms of TAK1 and TAB2 inhibit NF-kappaB activation induced by RANKL and endogenous TAK1 is activated in response to RANKL stimulation
Müller et al., Mol Cell Biol 2002 : In this work we used the liver derived MLP29 cell line to study the possible involvement of NF-kappaB in HGF/Met signaling ... In this work we used the liver derived MLP29 cell line to study the possible involvement of NF-kappaB in HGF/Met signaling ... HGF stimulates NF-kappaB DNA binding and transcriptional activation via the canonical IkappaB phosphorylation-degradation cycle and via the extracellular signal regulated kinase 1/2 and p38 mitogen activated protein kinase cascades ... Phosphatidylinositol 3-kinase is not involved in Met mediated NF-kappaB activation ... Phosphatidylinositol 3-kinase is not involved in Met mediated NF-kappaB activation
Gilston et al., Free radical research 2001 : NF-kappaB was activated by TNF-alpha in all four cell types tested
Haddad et al., Cell Signal 2002 : The regulation of cytokine gene transcription involves the reduction-oxidation ( redox ) -sensitive nuclear factor-kappaB (NF-kappaB), the activation of which is mediated through an upstream kinase that regulates the phosphorylation and subsequent degradation of inhibitory-kappaB (IkappaB)-alpha , the major cytosolic inhibitor of NF-kappaB ... In addition, BSO blockaded the phosphorylation of IkappaB-alpha , reduced its degradation, thereby allowing its cytosolic accumulation, and subsequently inhibited the activation of NF-kappaB
Marinari et al., Eur J Immunol 2002 : Searching for molecules that couple TCR and CD28 to MEKK1, we found that the guanine nucleotide exchange factor Vav synergized with CD28 stimulation in Jurkat cells to induce NF-kappaB transcriptional activity through the activation of IKKalpha and IKKbeta ... Searching for molecules that couple TCR and CD28 to MEKK1, we found that the guanine nucleotide exchange factor Vav synergized with CD28 stimulation in Jurkat cells to induce NF-kappaB transcriptional activity through the activation of IKKalpha and IKKbeta ... Finally, Vav induced NF-kappaB activation in CD28 costimulated cells was inhibited by dominant negative MEKK ( KM )
Fujihara et al., Eur J Immunol 2002 : Inhibition of TNF-alpha mediated IkappaBalpha degradation and NF-kappaB activation by gliotoxin or the proteasome inhibitor MG-132 un-masks the caspase dependent pro-apoptotic properties of TNF-alpha
Säemann et al., J Leukoc Biol 2002 : In particular, n-butyrate prevents homotypic DC clustering, inhibits IL-12 while sparing IL-10 production, and at the molecular level, blocks NF-kappa B translocation
Fiorentini et al., Mol Endocrinol 2002 (Pituitary Neoplasms...) : Moreover, NGF treatment of nonresponder cells induced both nuclear translocation and DNA binding activity of NF-kappaB complexes containing p50, p65/RelA, and cRel subunits, an effect prevented by anti-p75 ( NGFR ) antibodies ... These data indicate that in prolactinoma cells the effect of NGF on D ( 2 ) receptor expression is mediated by p75 ( NGFR ) in a trkA independent way and that NGF stimulation of p75 ( NGFR ) activates NF-kappaB , which is required for D ( 2 ) gene expression ... These data indicate that in prolactinoma cells the effect of NGF on D ( 2 ) receptor expression is mediated by p75 ( NGFR ) in a trkA independent way and that NGF stimulation of p75 ( NGFR ) activates NF-kappaB , which is required for D ( 2 ) gene expression
Webb et al., J Dent Res 2002 (Osteosarcoma) : Transfection of NFkappaB and C/EBPbeta-sensitive reporter promoter constructs demonstrated that NFkappaB activity was enhanced and that constitutive C/EBPbeta was inhibited by TNF-alpha, with both effects being p38 MAPK dependent ... Transfection of NFkappaB and C/EBPbeta-sensitive reporter promoter constructs demonstrated that NFkappaB activity was enhanced and that constitutive C/EBPbeta was inhibited by TNF-alpha , with both effects being p38 MAPK dependent
Rival et al., Eur J Pharmacol 2002 : PPARalpha and PPARdelta activators inhibit cytokine induced nuclear translocation of NF-kappaB and expression of VCAM-1 in EAhy926 endothelial cells
Park et al., J Biol Chem 2002 : Here we show that overexpression of NS5A inhibits tumor necrosis factor (TNF)-alpha induced nuclear factor kappaB (NF-kappaB) activation in HEK293 cells, as determined by luciferase reporter gene expression and by electrophoretic mobility shift assay ... NF-kappaB activation by TNF receptor associated death domain and TRAF2 was inhibited by NS5A, whereas MEKK1 and IKKbeta dependent NF-kappaB activation was not affected, suggesting that NS5A may inhibit NF-kappaB activation signaled by TRAF2 ... NF-kappaB activation by TNF receptor associated death domain and TRAF2 was inhibited by NS5A, whereas MEKK1 and IKKbeta dependent NF-kappaB activation was not affected, suggesting that NS5A may inhibit NF-kappaB activation signaled by TRAF2 ... NF-kappaB activation by TNF receptor associated death domain and TRAF2 was inhibited by NS5A, whereas MEKK1 and IKKbeta dependent NF-kappaB activation was not affected, suggesting that NS5A may inhibit NF-kappaB activation signaled by TRAF2 ... Our findings suggest a possible molecular mechanism that could explain the ability of NS5A to negatively regulate TNF-alpha induced NF-kappaB activation
Lim et al., J Immunol 2002 (MAP Kinase Signaling System) : To study the signaling events downstream of JNK activation, we show that dexamethasone did not inhibit LPS induced NF-kappaB activation in THP-1 cells, suggesting that JNK may not be involved in NF-kappaB activation leading to B7.2 expression
Ginis et al., J Cereb Blood Flow Metab 2002 (Brain Ischemia) : TNF-alpha activates a transcription factor NF-kappaB that binds promoters of multiple genes, thus ensuring pleiotropic effects of TNF-alpha
Chen et al., J Dent Res 2002 (Carcinoma, Squamous Cell...) : Here, we report that an adenovirus mediated gene transfer of NF-kappaB inhibitor, super-repressor I kappa B alpha ( Adv-SR-IkappaBalpha ), blocked TNF induced NF-kappaB activation and sensitized oral SCC cells to TNF killing ... Additionally, we found that the inhibition of NFkappaB by Adv-SR-IkappaBalpha enhanced TNF mediated caspase-8 and -3 activation
Bannerman et al., J Clin Invest 2002 : The Fas associated death domain protein suppresses activation of NF-kappa B by LPS and IL-1 beta ... The Fas associated death domain protein suppresses activation of NF-kappa B by LPS and IL-1 beta ... The Fas associated death domain protein suppresses activation of NF-kappa B by LPS and IL-1 beta ... Activation of NF-kappa B by bacterial LPS promotes the upregulation of proinflammatory cytokines that contribute to the pathogenesis of Gram negative septic shock ... LPS activation of NF-kappa B is dependent upon the interaction of two death domain containing ( DD-containing ) proteins, MyD88 and IL-1 receptor associated kinase IRAK ... Although FADD has been classically described as a proapoptotic signaling molecule, several reports have implicated a role for FADD in mediating NF-kappa B activation ... In the present report, we investigated whether FADD could mediate LPS activation of NF-kappa B ... In the present report, we investigated whether FADD could mediate LPS activation of NF-kappa B ... Overexpression of FADD blocked LPS induced NF-kappa B activation, whereas absence of FADD enhanced activation of NF-kappa B by LPS ... Overexpression of FADD blocked LPS induced NF-kappa B activation, whereas absence of FADD enhanced activation of NF-kappa B by LPS ... FADD ( -/- ) MEFs were also resistant to NF-kappa B activation induced by IL-1 beta ... Together, these data indicate that FADD negatively regulates LPS- and IL-1 beta induced NF-kappa B activation and that this regulation occurs upstream of I kappa B degradation ... Together, these data indicate that FADD negatively regulates LPS- and IL-1 beta induced NF-kappa B activation and that this regulation occurs upstream of I kappa B degradation
Kim et al., Biochem Biophys Res Commun 2002 : In addition, we previously found that cross linking of MHC class II molecules using anti-MHC class II antibodies inhibited NF-kappaB activation in resting B cells isolated from mouse spleen ... In this study, we investigated the mechanism of anti-MHC class II antibody mediated inhibition of LPS induced NF-kappaB activation using a resting B cell line, 38B9 ... Consequently, disruption of these pathways might contribute to the inhibition of LPS induced NF-kappaB activation in 38B9 cells
Mendes et al., Nitric Oxide 2002 : Role of mitogen activated protein kinases and tyrosine kinases on IL-1 Induced NF-kappaB activation and iNOS expression in bovine articular chondrocytes ... These responses to IL-1 were also inhibited by treatment of the cells with the tyrosine kinase inhibitors, genistein and tyrphostin B42, which also prevented IL-1 induced NF-kappaB activation ... The p38MAPK inhibitor, SB 203580, had no effect on IL-1 induced NF-kappaB activation ... The p38MAPK inhibitor, SB 203580, had no effect on IL-1 induced NF-kappaB activation ... The p38MAPK inhibitor, SB 203580, had no effect on IL-1 induced NF-kappaB activation ... In conclusion, this study shows that ( 1 ) PTK are part of the signaling pathway that leads to IL-1 induced NF-kappaB activation and iNOS expression ; ( 2 ) the p38MAPK cascade is required for IL-1 induced iNOS expression ; ( 3 ) the p42/44MAPK and AP-1 are not involved in IL-1 induced iNOS expression ; and ( 4 ) NF-kappaB and the p38MAPK lie on two distinct pathways that seem to be independently required for IL-1 induced iNOS expression
Höpken et al., Blood 2002 (Hodgkin Disease...) : CCR7 up-regulation was mediated by constitutive NF-kappaB activity
Bennasser et al., J Soc Biol 2001 : Stimulation by Tat induces nuclear translocation of the transcription factor NFkB the activation of which seems to be necessary for IL-10 production
Wright et al., Am J Physiol Heart Circ Physiol 2002 : However, interleukin-1beta and phorbol 12-myristate 13-acetate also activated NF-kappaB but did not evoke TNF-alpha expression, revealing that this factor is not sufficient for cytokine production ... However, interleukin-1beta and phorbol 12-myristate 13-acetate also activated NF-kappaB but did not evoke TNF-alpha expression, revealing that this factor is not sufficient for cytokine production
Mukhopadhyay et al., J Cell Biochem 2002 : Whether signal transducer and activator of transcription-1 ( STAT1 ), which mediates interferon ( IFN ) signaling, also plays any role in the TNF mediated activation of NF-kappaB , JNK, and apoptosis has not been established ... TNF activated NF-kappaB , consisting of p50 and p65, in both U3A and U3A-pSG91 cells in a dose- and time dependent manner, but the degree and rate of activation were slightly lower in U3A cells, as were IkappaBalpha degradation and NF-kappaB dependent reporter gene expression ... STAT1 was, however, required for IFNalpha mediated downregulation of TNF induced NF-kappaB activation ... STAT1 was, however, required for IFNalpha mediated downregulation of TNF induced NF-kappaB activation
Theuer et al., BMC cardiovascular disorders 2002 (Cardiomegaly...) : Our data show that inhibition of NF-kappaB by PDTC markedly reduces inflammation, iNOS expression in the dTGR most likely leading to decreased cytotoxicity, and cell proliferation
Volanti et al., Photochem Photobiol 2002 : This was further reinforced by the demonstration that herbimycin A, a tyrosine kinase inhibitor, prevented NF-kappaB activation by photosensitization but not by TNF alpha , a cytokine known to activate NF-kappaB through an IKK dependent mechanism
Kohn et al., Shock 2002 : Following heat shock, decreased TNF-alpha induced NF-kappaB activation was observed and was associated with preservation of IkappaB-alpha and a decrease in phosphorylated IkappaB-alpha that correlated to inhibition of I kappa kinase (IKK) activity
Berman et al., Clin Cancer Res 2002 (Carcinoma, Non-Small-Cell Lung...) : Recently, sulindac and other nonsteroidal anti-inflammatory drugs have been shown to inhibit tumor necrosis factor (TNF)-alpha mediated NF-kappaB activation ... We further show that sulindac inhibits TNF-alpha mediated activation of NF-kappaB DNA binding and nuclear translocation of NF-kappaB
Tsukamoto et al., Free Radic Biol Med 2002 (Liver Diseases) : Treatment of cultured HM with a lipophilic iron chelator ( deferiprone ) abrogates LPS induced NF-kappaB activation ... A moderate increase in the nonheme iron content in HM by erythrophagocytosis, promotes subsequent LPS stimulated NF-kappaB activation in a hemeoxygenase dependent manner ... Recent evidence also suggests a role of intracellular low molecular weight iron in the early signal transduction for LPS mediated NF-kappaB activation
Seno et al., Scand J Gastroenterol 2002 : TNF-alpha inhibition reduced the NF-kappaB activation, and blocked a transient wave of epithelial cell proliferation 12 h after the injection
Takaya et al., Scand J Gastroenterol 2002 : Furthermore, IL-17 induced a rapid increase in NF-kappaB DNA binding activity, and the combination of IL-17 and IFN-gamma further enhanced NF-kappaB DNA binding activity
Rothenberger et al., Cell Mol Life Sci 2002 : Moreover, Ndelta25 inhibited WT LMP1 mediated induction of the transcription factors NF-kappaB and AP-1
Coletti et al., EMBO J 2002 (Cachexia) : It has been proposed that TNFalpha mediated activation of NFkappaB leads to down regulation of MyoD, however the mechanisms underlying TNFalpha effects on skeletal muscle remain poorly understood
Ye et al., Neuroimmunomodulation 2001 : In subsequent studies, the effect of recombinant murine IL-10 on constitutive and inducible NFkappaB activity, IL-6 mRNA expression and IL-6 protein secretion by glia cultured from brains of adult and aged mice was determined
Spiegelman et al., Oncogene 2002 : In the NIH3T3 cells, which express HOS, but not betaTrCP, Wnt/beta-catenin signaling leads to inhibition of HOS promoter activity and NF-kappaB-driven transcription as well as to stabilization of beta-catenin ... In the NIH3T3 cells, which express HOS, but not betaTrCP, Wnt/beta-catenin signaling leads to inhibition of HOS promoter activity and NF-kappaB-driven transcription as well as to stabilization of beta-catenin
Tantini et al., FEBS Lett 2002 : Here we show an involvement of nuclear factor-kappaB (NF-kappaB) in the induction of nitric oxide synthase (NOS) and ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis ... Here we show an involvement of nuclear factor-kappaB (NF-kappaB) in the induction of nitric oxide synthase (NOS) and ornithine decarboxylase (ODC) , the key enzyme in polyamine biosynthesis
Kanekura et al., Biochim Biophys Acta 2002 : The involvement of NF-kappaB in the constitutive overexpression of cyclooxygenase-2 in cyclooxygenase-1 null cells
Hoshino et al., J Exp Med 2002 (HIV Infections...) : This is associated with loss of an inhibitory CCAAT enhancer binding protein beta ( C/EBPbeta ) transcription factor and activation of nuclear factor (NF)-kappaB ... This is associated with loss of an inhibitory CCAAT enhancer binding protein beta ( C/EBPbeta ) transcription factor and activation of nuclear factor (NF)-kappaB
Malhotra et al., Biochem Biophys Res Commun 2002 : There was no difference, however, in the ability of heat shock to inhibit TNFalpha mediated NF-kappaB activation , IkappaBalpha degradation, IkappaB kinase activation, and macrophage chemotactic protein-1 expression in the HSF-1-/- cells compared to the HSF-1+/+ cells
Shimamura et al., Exp Cell Res 2002 (Cell Transformation, Neoplastic...) : Furthermore, expression of a dominant negative mutant of IkappaBalpha, pMEIkappaBalpha67CJ, and a dominant negative mutant of p65, p65DeltaC , resulted in decreased focus formation and NF-kappaB activity ... Furthermore, expression of a dominant negative mutant of IkappaBalpha , pMEIkappaBalpha67CJ, and a dominant negative mutant of p65, p65DeltaC, resulted in decreased focus formation and NF-kappaB activity
Palanki et al., Curr Med Chem 2002 (Autoimmune Diseases) : In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, TNF alpha , and GM-CSF ... In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2, IL-3, IL-4, IL-5, IL-8, IL-13, TNF alpha, and GM-CSF ... In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2, IL-3, IL-4, IL-5, IL-8, IL-13 , TNF alpha, and GM-CSF ... In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2 , IL-3, IL-4, IL-5, IL-8, IL-13, TNF alpha, and GM-CSF ... In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2, IL-3 , IL-4, IL-5, IL-8, IL-13, TNF alpha, and GM-CSF ... In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2, IL-3, IL-4 , IL-5, IL-8, IL-13, TNF alpha, and GM-CSF ... In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2, IL-3, IL-4, IL-5 , IL-8, IL-13, TNF alpha, and GM-CSF ... In activated T cells, transcription factors such as the activator protein-1 (AP-1) regulate IL-2 production and production of matrix metalloproteinases, the nuclear factor kappa B (NF-kappa B) is essential for the transcriptional regulation of the proinflammatory cytokines IL-1, IL-6, IL-8 and TNF alpha, and nuclear factor of activated T-cells ( NFAT ) is required for the transcriptional regulation of IL-2, IL-3, IL-4, IL-5, IL-8 , IL-13, TNF alpha, and GM-CSF
Endo et al., J Virol 2002 (Cell Transformation, Viral) : Activation of cellular genes by Tax1 through transcription factor NF-kappa B is reportedly essential for the transformation of Rat-1 cells
Chen et al., Mol Cell 2002 : The IKK complex , containing two catalytic subunits IKKalpha and IKKbeta and a regulatory subunit NEMO, plays central roles in signal dependent activation of NF-kappaB ... Geldanamycin ( GA ), an antitumor agent that disrupts the formation of this heterocomplex, prevents TNF induced activation of IKK and NF-kappaB
Pahlevan et al., J Antimicrob Chemother 2002 : Potential of rifamides to inhibit TNF induced NF-kappaB activation
Rothwarf et al., Science's STKE : signal transduction knowledge environment 1999 : Gene knockout studies indicate that IKKbeta is primarily responsible for the activation of NF-kappaB in response to proinflammatory stimuli, whereas IKKalpha is essential for keratinocyte differentiation
Ruan et al., Zhonghua Bing Li Xue Za Zhi 2000 : Furthermore, anisodamine could block LPS induced NF-kappaB of HUVECs nuclear translocation
Zingarelli et al., FASEB J 2002 (Myocardial Infarction...) : These events were preceded by degradation of inhibitor kappaBalpha ( IkappaBalpha ), activation of IkappaB kinase complex (IKK) and c-Jun-NH2-terminal kinase (JNK) , and subsequently activation of nuclear factor-kappaB (NF-kappaB) and activator protein 1 (AP-1) as early as 15 min after reperfusion ... These events were preceded by degradation of inhibitor kappaBalpha ( IkappaBalpha ), activation of IkappaB kinase complex (IKK) and c-Jun-NH2-terminal kinase (JNK), and subsequently activation of nuclear factor-kappaB (NF-kappaB) and activator protein 1 (AP-1) as early as 15 min after reperfusion
Baron et al., Blood 2002 (Leukemia, Myelogenous, Chronic, BCR-ABL Positive) : Interestingly, inhibition of NF-kappaB activation by BAY11-67082 ( a specific NF-kappaB inhibitor ) resulted in down-regulation of ICAM-1 expression and a subsequent decrease in NK-induced killing of UT-7/9 transfectants
Mifflin et al., Am J Physiol Cell Physiol 2002 (Colitis) : Activation of nuclear factor-kappaB (NF-kappaB) , extracellular signal regulated protein kinase ( ERK ), p38, and protein kinase C ( PKC ) signaling pathways was each necessary for optimal COX-2 induction ... In contrast to what occurs in other cell types, including other myofibroblasts such as renal mesangial cells, PKC inhibition did not prevent IL-1 induced NF-kappaB or mitogen activated protein kinase/ stress activated protein kinase activation, suggesting a novel role for PKC isoforms during this process
Brar et al., Am J Physiol Lung Cell Mol Physiol 2002 : NADPH oxidase promotes NF-kappaB activation and proliferation in human airway smooth muscle ... Inhibition of NADPH oxidase activity with DPI prevented serum induced activation of nuclear factor-kappaB (NF-kappaB) , and overexpression of a superrepressor form of the NF-kappaB inhibitor IkappaBalpha significantly reduced human AWSM growth ... Inhibition of NADPH oxidase activity with DPI prevented serum induced activation of nuclear factor-kappaB (NF-kappaB) , and overexpression of a superrepressor form of the NF-kappaB inhibitor IkappaBalpha significantly reduced human AWSM growth
Majumdar et al., J Immunol 2002 : Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester ... The treatment of these cells with thalidomide suppressed TNF induced NF-kappaB activation, with optimum effect occurring at 50 microg/ml thalidomide ... Thalidomide suppressed H ( 2 ) O ( 2 ) -induced NF-kappaB activation but had no effect on NF-kappaB activation induced by PMA, LPS , okadaic acid, or ceramide, suggesting selectivity in suppression of NF-kappaB ... The suppression of TNF induced NF-kappaB activation by thalidomide correlated with partial inhibition of TNF induced degradation of an inhibitory subunit of NF-kappaB ( IkappaBalpha ), abrogation of IkappaBalpha kinase activation, and inhibition of NF-kappaB dependent reporter gene expression ... Overall, our results clearly demonstrate that thalidomide suppresses NF-kappaB activation specifically induced by TNF and H ( 2 ) O ( 2 ) and that this may contribute to its role in suppression of proliferation, inflammation, angiogenesis, and the immune system
Gomi et al., J Immunol 2002 : ( R ) -Flavolipin, in which the configuration of the lipid moiety is R, induced NF-kappaB activation via the TLR4-MD-2 complex , but ( S ) -flavolipin did not
McEvoy et al., J Immunol 2002 (Arthritis, Rheumatoid) : Activation of nuclear orphan receptor NURR1 transcription by NF-kappa B and cyclic adenosine 5'-monophosphate response element binding protein in rheumatoid arthritis synovial tissue ... IL-1beta- and TNF-alpha induced NF-kappaB binding to this site is due predominantly to p65-p50 heterodimer and p50 homodimer subunit protein complexes ... IL-1beta- and TNF-alpha induced NF-kappaB binding to this site is due predominantly to p65-p50 heterodimer and p50 homodimer subunit protein complexes
Espinosa et al., J Cell Sci 2002 : We have now demonstrated that the increase in p65-NFkappaB protein levels enhances Notch mediated activation of the Hes1 promoter up to three-fold
Zaheer et al., Neurochem Res 2001 : The activations of CREB and NF-kappaB were blocked by inhibitors of either p38 ( SB-203580 ) or MEK ( PD-098059 ), suggesting that they were events downstream of MAK kinase ... The inductions of BDNF and NGF were also blocked by inhibitors of p38 and MEK, as well as by the inhibition of NF-kappaB with a decoy DNA sequence ... The inductions of BDNF and NGF were also blocked by inhibitors of p38 and MEK, as well as by the inhibition of NF-kappaB with a decoy DNA sequence
Goletti et al., Cytokine 2002 : IL-4 inhibited LTR-driven transcription triggered by PMA stimulation of U1 cells, and this effect was dependent upon intact NF-kappaB but not Sp-1 binding sites
Majumder et al., Virology 2002 : Furthermore, NS5A prevented the association between TRADD and FADD and blocked TRADD mediated NF-kappaB activation
Loewe et al., J Invest Dermatol 2001 (Psoriasis) : Using CD62E, NF-kappa B, or AP-1-responsive promoter constructs, dimethylfumarate inhibited tumor-necrosis-factor induced activation of the CD62E and the NF-kappa B but not the AP-1 promoter construct
Mann et al., J Biol Chem 2002 : CD40 induces interleukin-6 gene transcription in dendritic cells : regulation by TRAF2, AP-1, NF-kappa B , AND CBF1 ... CD40 induces interleukin-6 gene transcription in dendritic cells : regulation by TRAF2, AP-1, NF-kappa B , AND CBF1
Chin et al., Nature 2002 (Listeriosis...) : Rip2-deficient macrophages infected with L. monocytogenes or treated with lipopolysaccharide (LPS) have decreased activation of NF-kappaB, whereas dominant negative Rip2 inhibited NF-kappaB activation mediated by Toll-like receptor 4 and Nod1 ... Rip2-deficient macrophages infected with L. monocytogenes or treated with lipopolysaccharide (LPS) have decreased activation of NF-kappaB, whereas dominant negative Rip2 inhibited NF-kappaB activation mediated by Toll-like receptor 4 and Nod1 ... Rip2-deficient macrophages infected with L. monocytogenes or treated with lipopolysaccharide (LPS) have decreased activation of NF-kappaB, whereas dominant negative Rip2 inhibited NF-kappaB activation mediated by Toll-like receptor 4 and Nod1
Dhanalakshmi et al., Oncogene 2002 (Prostatic Neoplasms) : Silibinin inhibits constitutive and TNFalpha induced activation of NF-kappaB and sensitizes human prostate carcinoma DU145 cells to TNFalpha induced apoptosis ... Additional studies showed that silibinin also inhibits TNFalpha induced activation of NF-kappaB via IkappaBalpha pathway and subsequently sensitizes DU145 cells to TNFalpha induced apoptosis
Kotake et al., Shock 2002 (Endotoxemia) : Gel-shift and immunohistochemical analyses indicated that the transcription factor NF-kappaB was deactivated after PBN treatment, suggesting that NF-kappaB deactivation is closely involved in IL-10 overexpression
Alaoui-El-Azher et al., Mol Pharmacol 2002 : Both AA and ETYA inhibited basal activation of NF-kappaB but had no effect on LPS induced NF-kappaB translocation, suggesting that suppression of sPLA ( 2 ) -IIA synthesis by AA in LPS stimulated cells occurs via a NF-kappaB independent pathway
Aupperle et al., Cell Immunol 2001 (Arthritis, Rheumatoid...) : IkappaB kinase (IKK) plays a key role in the regulation of nuclear factor kappaB (NF-kappaB)
Hu et al., Biochem Biophys Res Commun 2002 (Calcium Signaling) : This study shows that activation of PLD by histamine regulates [ Ca ( 2+ ) ] ( i ) oscillation frequency and NF-kappaB activity in HAEC
Rodríguez et al., J Virol 2002 : Thus, transient transfection of the viral IAP increases the activity of an NF-kappaB reporter gene in a dose-responsive manner in Jurkat cells ... The NF-kappaB inducing activity of ASFV IAP was abrogated by an IKK-2 dominant negative mutant and enhanced by expression of tumor necrosis factor receptor associated factor 2
Jackson et al., Bioorg Med Chem Lett 2002 (Lung Neoplasms) : The compounds display good potency in inhibiting TNF-alpha induced apoptosis and NF kappa B activation
Méndez-Samperio et al., J Interferon Cytokine Res 2002 : Finally, two specific NF-kappa B inhibitors, sulfasalazine and caffeic acid phenetyl ester ( CAPE ), strongly inhibited the production of CXCL-8 by human monocytes infected with M. bovis
Pizzi et al., J Biol Chem 2002 : In both cultured rat cerebellar granule cells and mouse hippocampal slices, we examined NF-kappaB/Rel activation induced by two opposing modulators of cell viability : 1 ) interleukin-1beta (IL-1beta) , which promotes neuron survival and 2 ) glutamate, which can elicit toxicity
Aramaki et al., Biol Pharm Bull 2002 : These results indicated that NADPH-oxidase plays a crucial role in macrophage activation by CpG-ODN and NF-kappaB does not act as a predominant transcription factor for IL-12 secretion
Okuno et al., Scand J Gastroenterol 2002 : The inhibition of NF-kappaB activation markedly blocked both IL-1beta- and TNF-alpha induced IL-8 and MCP-1 mRNA expression, but did not affect MMP-1 mRNA expression ... The inhibition of NF-kappaB activation markedly blocked both IL-1beta- and TNF-alpha induced IL-8 and MCP-1 mRNA expression, but did not affect MMP-1 mRNA expression ... The inhibition of NF-kappaB activation markedly blocked both IL-1beta- and TNF-alpha induced IL-8 and MCP-1 mRNA expression, but did not affect MMP-1 mRNA expression
James et al., Diabetes 2002 (Hyperinsulinism) : Overexpression of glutamine : fructose-6-phosphate amidotransferase ( GFAT ), the rate limiting enzyme for flux through the hexosamine pathway, led to a 2.2-fold increase in NF-kappaB enhancer activity ; the combination of GFAT overexpression and high glucose increased activity 2.8-fold, and these increases were prevented by 40 micromol/l O-diazoacetyl-L-serine ( azaserine ) or 6-diazo-5-oxonorleucine
Yamamoto et al., J Bone Miner Res 2002 : IKK2DN and MKK7DN overexpression in RAW cells specifically suppressed the NF-kappaB activation and JNK activation in response to RANKL, respectively, without affecting other signaling pathways ... IKK2DN and MKK7DN overexpression in RAW cells specifically suppressed the NF-kappaB activation and JNK activation in response to RANKL , respectively, without affecting other signaling pathways
Clohisy et al., J Orthop Res 2002 (Osteolysis...) : The nuclear transcription factor NF-kappaB mediates TNF signaling and this transcription complex is necessary for osteoclastogenesis
Okajima et al., Immunol Rev 2001 (Inflammation) : APC directly inhibits the production of TNF-alpha by inhibiting the activation of both nudear factor kappaB (NFkappaB) and activator protein-1 in monocytes stimulated with LPS
Ammit et al., Am J Respir Cell Mol Biol 2002 : beta-Agonists augmented TNF-alpha induced IL-6 secretion, reflecting an additive effect of NF-kappaB and CRE response elements on IL-6 gene expression
Reuter et al., Ann Neurol 2002 (Migraine Disorders) : iNOS expression is preceded by significant nuclear factor kappa B (NF-kappaB) activity, as reflected by a reduction in the inhibitory protein-kappa-Balpha ( IkappaBalpha ) and activation of NF-kappaB after GTN infusion
Carpenter et al., BMC infectious diseases 2002 (Lung Neoplasms) : Respiratory syncytial virus and TNF alpha induction of chemokine gene expression involves differential activation of Rel A and NF-kappa B1 ... In this study, we examined the NF-kappaB mediated effects of RSV and the proinflammatory cytokine TNFalpha on the induction of IL-8, MCP-1 and RANTES chemokine gene expression in A549 epithelial cells ... In this study, we examined the NF-kappaB mediated effects of RSV and the proinflammatory cytokine TNFalpha on the induction of IL-8 , MCP-1 and RANTES chemokine gene expression in A549 epithelial cells ... In this study, we examined the NF-kappaB mediated effects of RSV and the proinflammatory cytokine TNFalpha on the induction of IL-8, MCP-1 and RANTES chemokine gene expression in A549 epithelial cells ... DNA binding studies using NF-kappaB subunit specific binding ELISA demonstrated that RSV and TNFalpha induced different NF-kappaB binding complexes containing Rel A ( p65 ) and NF-kappaB1 ( p50 )
Asai et al., Jpn J Cancer Res 2002 (Bone Neoplasms...) : Therefore, decrease of p-IkappaBalpha degrading activity might be responsible for the decrease in NFkappaB activation
Bentires et al., Bull Mem Acad R Med Belg 2001 (Neoplasms) : We investigated the role of NF-kappa B in the regulation of the pro-apoptotic protein Bax
Ohkita et al., Jpn J Pharmacol 2002 : Electrophoretic mobility shift assay showed that proteasome inhibitors diminished TNF-alpha stimulated nuclear factor-kappa B (NF-kappaB) activation in ECs
He et al., Zhonghua Yi Xue Za Zhi 2001 (Pulmonary Disease, Chronic Obstructive) : The constitutive activity of AP-1 and the activity of NF kappa B induced by LPS were higher in the patients with COPD than in the controls ... Enhancement of activity of NF kappa B and AP-1 may positively regulate the production of IL-8 and IL-1 beta in the airflow obstruction ... Enhancement of activity of NF kappa B and AP-1 may positively regulate the production of IL-8 and IL-1 beta in the airflow obstruction
Reyes-Reyes et al., Biochem J 2002 : However, PI-3K was required for beta1 integrin-, but not beta2 integrin-, mediated NF-kappaB activation ... However, PI-3K was required for beta1 integrin-, but not beta2 integrin-, mediated NF-kappaB activation ... In addition, inhibition of PI-3K with wortmannin and LY294002 blocked beta1 integrin mediated NF-kappaB activation, but did not affect that mediated by beta2 integrin
He et al., Acta Pharmacol Sin 2002 : PGA1 can inhibit rat cardiac microvascular endothelial cell apoptosis by inhibiting activity of NF-kappaB
Zhong et al., Mol Cell 2002 : Only the p50-HDAC-1 complexes bind to DNA and suppress NF-kappa B-dependent gene expression in unstimulated cells
Eliopoulos et al., J Virol 2002 (Carcinoma...) : The oncogenic protein kinase Tpl-2/Cot contributes to Epstein-Barr virus encoded latent infection membrane protein 1-induced NF-kappaB signaling downstream of TRAF2 ... The oncogenic protein kinase Tpl-2/Cot contributes to Epstein-Barr virus encoded latent infection membrane protein 1-induced NF-kappaB signaling downstream of TRAF2 ... LMP1 promotes NF-kappaB activation through the recruitment of the adapter protein TRAF2 and the formation of a dynamic multiprotein complex that includes the NF-kappaB kinase, the IkappaB kinases, and their downstream targets, IkappaBs and p105 ... In this study, we have identified the oncogenic kinase Tpl-2/Cot as a novel component of LMP1 induced NF-kappaB signaling ... Inducible expression of LMP1 promotes the activation of Tpl-2, and a catalytically inactive Tpl-2 mutant suppresses LMP1 induced NF-kappaB signaling ... Consistent with this observation, catalytically inactive Tpl-2 also blocked CD40 mediated NF-kappaB activation, which largely depends on TRAF2 ... Consistent with this observation, catalytically inactive Tpl-2 also blocked CD40 mediated NF-kappaB activation, which largely depends on TRAF2 ... The ability of Tpl-2 to influence LMP1 induced NF-kappaB occurs through modulation of both IkappaBalpha and p105 functions
Knorre et al., Biol Chem 2002 (Cystic Fibrosis...) : DeltaF508-CFTR causes constitutive NF-kappaB activation through an ER-overload response in cystic fibrosis lungs
Li et al., Chin Med J (Engl) 2002 : Effect of NF-kappaB on the induction of PDGF-B transcription by angiotensin II in the ECV304 cell line ... To examine the effect of angiotensin II (Ang II) on nuclear factor-kappa B (NF-kappaB) activation in human endothelial cell line ECV304 and the molecular mechanism by which Ang II activates NF-kappaB ... To examine the effect of angiotensin II (Ang II) on nuclear factor-kappa B (NF-kappaB) activation in human endothelial cell line ECV304 and the molecular mechanism by which Ang II activates NF-kappaB
McFarlane et al., FEBS Lett 2002 : TNF activates pro-inflammatory transcription factor nuclear factor-kappaB (NF-kappaB) by uncertain signalling mechanisms
Ko et al., FEBS Lett 2002 (Osteosarcoma) : Nuclear factor-kappaB (NF-kappaB) inhibitor reduced chemotactic activity of Lkn-1 ... DNA binding activity of NF-kappaB was also enhanced by Lkn-1 stimulation
Wu et al., Am J Physiol Lung Cell Mol Physiol 2002 (MAP Kinase Signaling System) : EGFR signaling appears to cross talk with NF-kappaB signaling at the level of Ras, but additional signals appear necessary for NF-kappaB activation
Poynter et al., Am J Pathol 2002 (Bronchial Hyperreactivity...) : IKK activation was followed by nuclear localization of nuclear factor (NF)-kappaB within the bronchiolar epithelium and increased luciferase activity in lungs of mice containing a NF-kappaB dependent reporter gene
Yang et al., J Neurosci 2002 : Moreover, little nuclear factor-kappaB (NF-kappaB) translocation is induced by TNF-alpha in neurons of TNFRI -/-, whereas NF-kappaB subunit p65 is still translocated from the cytoplasm into the nucleus in neurons from wild-type and TNFRII -/- mice
Meiler et al., J Mol Cell Cardiol 2002 : The serine-threonine kinase , IKK beta, can mediate NF- kappa B activation although several alternative pathways exist
Kim et al., J Cell Biochem 2002 : DeltaIkappaBalpha suppressed the transactivation of NF-kappaB induced by TNF-alpha or TRAIL, as reflected by luciferase-reporter activity ... DeltaIkappaBalpha suppressed the transactivation of NF-kappaB induced by TNF-alpha or TRAIL , as reflected by luciferase-reporter activity ... Conversely, expression of the p65 subunit of NF-kappaB suppressed TNF-alpha- , TRAIL-, and serum deprivation induced cell death ... Conversely, expression of the p65 subunit of NF-kappaB suppressed TNF-alpha-, TRAIL- , and serum deprivation induced cell death
Sidell et al., Ann N Y Acad Sci 2002 (Endometriosis) : Our studies include a number of relevant findings : ( 1 ) RU486, acting as an antioxidant, can suppress activation of NFkappaB , a nuclear transcription factor that affects the expression of several inflammatory genes such as those for MCP-1, GM-CSF, CSF-1, and various adhesion molecules ; ( 2 ) IL-6 secretion from a variety of cell types including endometrial cells is inhibited by retinoic acid ; and ( 3 ) retinoids and PPARgamma ligands can upregulate the expression of scavenger receptors in cells of the monocyte/macrophage lineage
Lehmann et al., J Rheumatol 2002 (Osteoarthritis) : We determined the effect of glucocorticoids and tumor necrosis factor (TNF) on nuclear localization and DNA binding of the transcription factor NF-kappaB in osteoarthritic ( OA ) synovial tissue ... TNF induced NF-kappaB nuclear translocation and DNA binding in all OA synovial tissue explants, although there were no consistent effects on AP-1 DNA binding ... Dexamethasone partially inhibits TNF induced NF-kappaB DNA binding in human synovial tissue
Swinney et al., J Biol Chem 2002 : We propose that Ro106-9920 selectively inhibits an uncharacterized but essential ubiquitination activity associated with LPS- and TNFalpha induced IkappaB(alpha) degradation and NF-kappaB activation
Zhang et al., Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2002 : The induction of IL-8 gene expression by fluid shear stress is probably due to the activation of NF-kappa B
Gan et al., Zhonghua Yi Xue Za Zhi 2002 (Colitis, Ulcerative) : Glucocorticoids and SASP strongly inhibited NF-kappaB activation and expression
Dahan et al., Infect Immun 2002 (Escherichia coli Infections) : These findings demonstrate that ( i ) EHEC can induce in vitro a potent proinflammatory response by secretion of IL-8 and ( ii ) the secretion of IL-8 is due to the involvement of MAPK, AP-1, and NF-kappaB signaling pathways
Wong et al., Shock 2002 (Hyperoxia...) : Hyperoxia prolongs tumor necrosis factor-alpha mediated activation of NF-kappaB : role of IkappaB kinase ... Hyperoxia prolongs tumor necrosis factor-alpha mediated activation of NF-kappaB : role of IkappaB kinase ... Stimulation with TNFalpha alone increased NF-kappaB activation within 1 h and induced IkappaB alpha degradation within 0.5 h ... These data demonstrate that while hyperoxia alone does not affect activation of NF-kappaB, hyperoxia prolongs TNFalpha mediated activation of NF-kappaB
Zhang et al., Int J Oncol 2002 : Furthermore, NDPP1 is able to inhibit NF-kappaB activation induced by TNF-alpha treatment in HepG2 cells
Pajonk et al., Free Radic Biol Med 2002 : NAC blocked radiation induced NF-kappaB activity in ECV 304 cells and RAW 264.7 macrophages, as measured by a gel shift assay, at doses that inhibited proteasome activity ... This provides a possible mechanism whereby NAC could block NF-kappaB activation and affect the expression of other molecules that are dependent on the ubiquitin/proteasome system for their degradation, other than by scavenging free radicals
Li et al., Proc Natl Acad Sci U S A 2002 (MAP Kinase Signaling System) : Endogenous IRAK-4 interacts with IRAK-1 and TRAF6 in an IL-1 dependent manner, and overexpression of IRAK-4 can activate NF-kappa B as well as mitogen activated protein ( MAP ) kinase pathways
Kuo et al., Oncogene 2002 (Carcinoma, Hepatocellular) : 2-AAF activates IkappaB kinase (IKK), resulting in degradation of IkappaBbeta and activation of NF-kappaB
Bhat-Nakshatri et al., Oncogene 2002 (Breast Neoplasms) : Using breast cancer cell lines derived from transgenic mice that overexpress specific oncogene/growth factors in the mammary gland, we show that heregulin but not her2/neu, c-Myc or v-Ha-ras plays a major role in constitutive NF-kappaB activation ... Using breast cancer cell lines derived from transgenic mice that overexpress specific oncogene/growth factors in the mammary gland, we show that heregulin but not her2/neu, c-Myc or v-Ha-ras plays a major role in constitutive NF-kappaB activation ... Her2/neu potentiated tumor necrosis factor alpha (TNFalpha)-inducible NF-kappaB activation whereas c-Myc potentiated 12-o-tetracecanyolphorbol-13-acetate ( TPA ) -induced NF-kappaB activation ... Tryphostin AG1517, which inhibits heregulin mediated phosphorylation of EGFR, her2/neu and ErbB3 reduced NF-kappaB activation
Chang et al., BMC cell biology 2002 : TNF activated NF-kappaB , along with IkappaBalpha degradation, occurred at 20 to 60 min in Hyal-2 cells post stimulation, but at the 20 min time point in both control and Hyal-1 cells
Feng et al., Am J Physiol Gastrointest Liver Physiol 2002 (Necrosis) : STS activated I kappa B kinase and nuclear factor-kappa B (NF-kappa B) nuclear translocation and DNA binding but inhibited transactivation of I kappa B-alpha, inducible nitric oxide synthase, and inhibitor of apoptosis protein-1 in hepatocytes and NF-kappa B reporter in transfected Huh-7 cells
Lorenzo et al., J Am Soc Nephrol 2002 : In cultured rat mesangial cells, both AT(1) and AT(2) receptor antagonists inhibited AngII induced NF-kappaB DNA binding activity, whereas NF-kappaB activation elicited by AngIII was mainly blocked by the AT(2) receptor antagonist ... These data demonstrate that, in mesangial cells, NF-kappaB activation is mediated by AT(1) and AT(2) receptors, suggesting a novel intracellular signaling mechanism for AT(2) receptors in the kidney ... These data demonstrate that, in mesangial cells, NF-kappaB activation is mediated by AT(1) and AT(2) receptors, suggesting a novel intracellular signaling mechanism for AT(2) receptors in the kidney
McInnis et al., J Pharmacol Exp Ther 2002 (Necrosis) : Time course studies and experiments with SN50 and M40403 suggest that O ( 2 ) production and NF-kappaB translocation may be involved in necrosis and apoptosis, but the latter also requires an increased expression of Bax
Gu et al., Blood 2002 (Precursor Cell Lymphoblastic Leukemia-Lymphoma) : MDM2 induces NF-kappaB/p65 expression transcriptionally through Sp1 binding sites : a novel, p53 independent role of MDM2 in doxorubicin resistance in acute lymphoblastic leukemia ... MDM2 induces NF-kappaB/p65 expression transcriptionally through Sp1 binding sites : a novel, p53 independent role of MDM2 in doxorubicin resistance in acute lymphoblastic leukemia
Zhan et al., Cell Death Differ 2002 : Death effector domain containing proteins are involved in important cellular processes such as death-receptor induced apoptosis, NF-kappaB activation and ERK activation
Choe et al., J Virol 2002 : Activation of NF-kappaB by R5 and X4 human immunodeficiency virus type 1 induces macrophage inflammatory protein 1alpha and tumor necrosis factor alpha in macrophages
Hour et al., Prostate 2002 (Prostatic Neoplasms) : Moreover, CCM was able to inhibit both the constitutional and TNF-alpha induced NF-kappaB activation in a time dependent manner
Loewe et al., J Immunol 2002 : We show that DMF inhibits TNF induced tissue factor mRNA and protein expression as well as TNF induced DNA binding of NF-kappa B proteins, but not VEGF induced tissue factor protein, mRNA expression, or VEGF induced early gene response-1 transcription factor/DNA binding
Horie et al., Oncogene 2002 (Hodgkin Disease) : We report that in H-RS cells overexpression of CD30 leads to self-aggregation, recruitment of TRAF2 and TRAF5, and NF-kappaB activation, independent of CD30 ligand ... We report that in H-RS cells overexpression of CD30 leads to self-aggregation, recruitment of TRAF2 and TRAF5, and NF-kappaB activation , independent of CD30 ligand ... We report that in H-RS cells overexpression of CD30 leads to self-aggregation, recruitment of TRAF2 and TRAF5 , and NF-kappaB activation , independent of CD30 ligand
Wu et al., Mol Cell Biol 2002 : We demonstrated that SRC-3 was able to enhance NF-kappa B-mediated gene expression in concert with IKK ... Finally, the hypothesis that SRC-3 is involved in NF-kappa B-mediated gene expression is further supported by the reduced expression of interferon regulatory factor 1, a well-known NF-kappa B target gene, in the spleens of SRC-3 null mutant mice
An et al., Immunology 2002 : Involvement of ERK, p38 and NF-kappaB signal transduction in regulation of TLR2 , TLR4 and TLR9 gene expression induced by lipopolysaccharide in mouse dendritic cells ... Involvement of ERK, p38 and NF-kappaB signal transduction in regulation of TLR2, TLR4 and TLR9 gene expression induced by lipopolysaccharide in mouse dendritic cells ... Involvement of ERK, p38 and NF-kappaB signal transduction in regulation of TLR2, TLR4 and TLR9 gene expression induced by lipopolysaccharide in mouse dendritic cells ... While inhibition of extracellular signal related kinase and NF-kappaB activation suppressed the up-regulation of the expression of TLR2, TLR4 and TLR9 mRNA, inhibition of p38 kinase prevented the up-regulation of TLR2 and TLR4 mRNA expression but enhanced the up-regulation of TLR9 expression ... While inhibition of extracellular signal related kinase and NF-kappaB activation suppressed the up-regulation of the expression of TLR2 , TLR4 and TLR9 mRNA, inhibition of p38 kinase prevented the up-regulation of TLR2 and TLR4 mRNA expression but enhanced the up-regulation of TLR9 expression ... While inhibition of extracellular signal related kinase and NF-kappaB activation suppressed the up-regulation of the expression of TLR2, TLR4 and TLR9 mRNA, inhibition of p38 kinase prevented the up-regulation of TLR2 and TLR4 mRNA expression but enhanced the up-regulation of TLR9 expression ... While inhibition of extracellular signal related kinase and NF-kappaB activation suppressed the up-regulation of the expression of TLR2, TLR4 and TLR9 mRNA, inhibition of p38 kinase prevented the up-regulation of TLR2 and TLR4 mRNA expression but enhanced the up-regulation of TLR9 expression
Chen et al., J Biol Chem 2002 : In this study, we show the roles that CaMKK and Akt play in regulating interleukin-1beta (IL-1beta) induced NF-kappaB signaling ... In human embryonic kidney 293 cells, IL-1beta induces IkappaB kinase beta (IKKbeta) activation, IkappaBalpha degradation, NF-kappaB transactivation , and weak Akt activation ... In human embryonic kidney 293 cells, IL-1beta induces IkappaB kinase beta (IKKbeta) activation, IkappaBalpha degradation, NF-kappaB transactivation , and weak Akt activation ... In human embryonic kidney 293 cells, IL-1beta induces IkappaB kinase beta (IKKbeta) activation, IkappaBalpha degradation, NF-kappaB transactivation, and weak Akt activation ... In human embryonic kidney 293 cells, IL-1beta induces IkappaB kinase beta (IKKbeta) activation, IkappaBalpha degradation, NF-kappaB transactivation, and weak Akt activation ... In human embryonic kidney 293 cells, IL-1beta induces IkappaB kinase beta (IKKbeta) activation, IkappaBalpha degradation, NF-kappaB transactivation , and weak Akt activation ... A CaMKK inhibitor ( KN-93 ) and phosphatidylinositol 3-kinase inhibitors ( wortmannin and LY294002 ) do not inhibit IL-1beta induced NF-kappaB activation ... However, IL-1beta induced NF-kappaB activity is attenuated by increased intracellular calcium in response to ionomycin, UTP , or thapsigargin or by overexpression of CaMKKc and/or Akt ... However, IL-1beta induced NF-kappaB activity is attenuated by increased intracellular calcium in response to ionomycin, UTP, or thapsigargin or by overexpression of CaMKKc and/or Akt ... However, IL-1beta induced NF-kappaB activity is attenuated by increased intracellular calcium in response to ionomycin, UTP, or thapsigargin or by overexpression of CaMKKc and/or Akt ... We have also identified a novel interaction between CaMKK stimulated Akt and interleukin-1 receptor associated kinase 1 ( IRAK1 ), which plays a key role in IL-1beta induced NF-kappaB activation ... CaMKKc and Akt overexpression decreases IRAK1 mediated NF-kappaB activity and its association with MyD88 in response to IL-1beta stimulation ... CaMKKc and Akt overexpression decreases IRAK1 mediated NF-kappaB activity and its association with MyD88 in response to IL-1beta stimulation ... Furthermore, CaMKKc and Akt overexpression increases IRAK1 phosphorylation at Thr ( 100 ), and point mutation of this site abrogates the inhibitory effect of Akt on IRAK1 mediated NF-kappaB activation ... Taken together, these results indicate a novel regulatory mechanism for IL-1beta signaling and suggest that CaMKK dependent Akt activation inhibits IL-1beta induced NF-kappaB activation through interference with the coupling of IRAK1 to MyD88 ... Taken together, these results indicate a novel regulatory mechanism for IL-1beta signaling and suggest that CaMKK dependent Akt activation inhibits IL-1beta induced NF-kappaB activation through interference with the coupling of IRAK1 to MyD88 ... Taken together, these results indicate a novel regulatory mechanism for IL-1beta signaling and suggest that CaMKK dependent Akt activation inhibits IL-1beta induced NF-kappaB activation through interference with the coupling of IRAK1 to MyD88
Wang et al., Zhonghua Zhong Liu Za Zhi 2002 (Nasopharyngeal Neoplasms) : In the meantime, efforts were made to demonstrate if LMP1 regulates NF-kappa B or AP-1 activation using reporter gene analysis
Zamamiri-Davis et al., Free Radic Biol Med 2002 : The present study is based on the hypothesis that oxidant stress, a consequence of Se deficiency, lowers the activation potential of the redox-sensitive transcription factor, NF-kappaB, and that the activated NF-kappaB is required for the altered expression of COX-2
Ruan et al., Diabetes 2002 (Insulin Resistance) : 3T3-L1 adipocytes expressing IkappaBalpha-DN , a nondegradable NF-kappaB inhibitor , exhibited normal morphology, global gene expression, and insulin responses
Delerive et al., Mol Endocrinol 2002 : By contrast, DRIP205 is not involved in PPARalpha mediated NF-kappaB transcriptional repression
Kanayama et al., J Biol Chem 2002 : TauCl did not inhibit either the upstream of IkappaB kinase (IKK) activation or IKK itself but did inhibit NF-kappaB activation induced by IKK overexpression ... TauCl did not inhibit either the upstream of IkappaB kinase (IKK) activation or IKK itself but did inhibit NF-kappaB activation induced by IKK overexpression
Ariga et al., J Biol Chem 2002 : It also inhibited the TNF-alpha induced DNA binding of nuclear NF-kappaB but not the phosphorylation and degradation of IkappaB
Don et al., Mol Cell Endocrinol 2002 : In addition, TNFalpha secreted by round spermatids, activates NF-kappaB dependent CREB expression in Sertoli cells and thus, contributes to the elevated CREB levels as long as these cells are intimately associated
Szabo et al., Frontiers in bioscience : a journal and virtual library 2002 : Activation of NF-kappa B , however, is regulated at multiple levels including I-kappa B degradation, nuclear translocation, and by interaction of NF-kappa B/Rel with other transcription factors ... Inhibition of NF-kappa B activation by alcohol in monocytic cells was independent of I-kappa B alpha degradation ... These acute-alcohol induced changes in monocytic cells were different compared to T lymphocytes, both in Jurkat CD4 cells and peripheral human T cells, acute alcohol had a biphasic effect on TNF-alpha induced NF-kappa B activation via an I-kappa B alpha dependent mechanism
Friedman et al., J Virol 2002 : Inhibition of tumor necrosis factor alpha induced NF-kappa B activation by the adenovirus E3-10.4/14.5K complex ... We demonstrate here that the E3 region of Ad inhibits the activation of NF-kappa B induced by tumor necrosis factor alpha (TNF-alpha) and interleukin-1 ... We demonstrate here that the E3 region of Ad inhibits the activation of NF-kappa B induced by tumor necrosis factor alpha (TNF-alpha) and interleukin-1
Bosch et al., J Virol 2002 : We studied the transcriptional regulation of IL-8 in the ECV304 and 293T cell lines and found that the induction of IL-8 gene expression involved the activation of NF-kappa B ( P = 0.001 ) and, to a lesser extent, the activation of NFIL-6 in ECV304 cells only
Mori et al., Int J Cancer 2002 (Leukemia, T-Cell) : Inhibitors of NF-kappaB ( dominant negative IkappaBs mutants ) suppressed Tax dependent activation of cyclin D1 and D2 promoters, indicating that Tax induced activation was mediated by NF-kappaB
Bancroft et al., Int J Cancer 2002 (Carcinoma, Squamous Cell...) : In our study, we examined the effect of EGF and antagonists of EGFR, PI3K and MEK on NF-kappaB and AP-1 activation and IL-8 and VEGF expression in HNSCC cell lines UM-SCC-9 and 11B in which EGFR is overexpressed and activated ... Recombinant EGF induced EGFR phosphorylation, activation of NF-kappaB and AP-1 reporter genes and IL-8 and VEGF expression, indicating that EGFR can mediate coactivation of both transcription factors and cytokine genes in HNSCC ... Recombinant EGF induced EGFR phosphorylation, activation of NF-kappaB and AP-1 reporter genes and IL-8 and VEGF expression, indicating that EGFR can mediate coactivation of both transcription factors and cytokine genes in HNSCC ... Recombinant EGF induced EGFR phosphorylation, activation of NF-kappaB and AP-1 reporter genes and IL-8 and VEGF expression, indicating that EGFR can mediate coactivation of both transcription factors and cytokine genes in HNSCC ... Recombinant EGF induced EGFR phosphorylation, activation of NF-kappaB and AP-1 reporter genes and IL-8 and VEGF expression, indicating that EGFR can mediate coactivation of both transcription factors and cytokine genes in HNSCC ... We conclude that antagonists of EGFR, PI3K and MEK signal pathways have inhibitory activity against EGFR induced NF-kappaB and AP-1 activation, IL-8 and VEGF expression and growth by HNSCC
Gewirtz et al., J Immunol 2002 (Colitis) : Nanomolar concentrations of LXA ( 4 ) analog reduced NF-kappaB mediated transcriptional activation in a LXA(4) receptor dependent manner and inhibited induced degradation of IkappaBalpha
Yeh et al., Biochem Pharmacol 2002 (Uterine Cervical Neoplasms) : The activation of NF kappa B by cisplatin or doxorubicin was not due to the degradation of cytoplasmic I kappa B alpha , as demonstrated by western blotting
Takahashi et al., Atherosclerosis 2002 : In addition, IL-1beta induced NF-kappaB activation in VSMC, an effect that was increased by the addition of beta-VLDL ... In addition, IL-1beta induced NF-kappaB activation in VSMC, an effect that was increased by the addition of beta-VLDL
Schroeder et al., Proc Natl Acad Sci U S A 2002 : CFTR is a pattern recognition molecule that extracts Pseudomonas aeruginosa LPS from the outer membrane into epithelial cells and activates NF-kappa B translocation
Guillén et al., J Biol Chem 2002 : Here we present molecular data demonstrating that both PTHrP ( 1-36 ) and PTHrP ( 107-139 ) activate NF-kappaB , leading to an increase in IL-6 mRNA, in these cells
Sasaki et al., BMC gastroenterology 2002 : Our data indicate that melatonin may exert therapeutic activity in IBD through its ability to inhibit NF-kappaB dependent induction of MAdCAM-1
Ortiz et al., Am J Physiol Lung Cell Mol Physiol 2002 (Body Weight...) : Enalapril protects mice from pulmonary hypertension by inhibiting TNF mediated activation of NF-kappaB and AP-1
Day et al., Am J Physiol Lung Cell Mol Physiol 2002 : In contrast, NF-kappaB activation by bleomycin is inhibited by U-0126, but not by NAC
Escalante et al., Structure 2002 : Upon viral infection, NF-kappaB translocates to the nucleus and activates the IFN-beta gene by binding to the PRDII element
Prabhu et al., Biochem J 2002 : Electrophoretic mobility-shift assays, nuclear factor-kappaB (NF-kappaB)-luciferase reporter assays and Western blot analyses indicate that the increased expression of iNOS in Se deficiency could be due to an increased activation and consequent nuclear localization of the redox-sensitive transcription factor NF-kappaB
Chuang et al., Biochem Pharmacol 2002 (Drug Hypersensitivity) : In contrast to tumor necrosis factor-alpha (TNF-alpha) , which activates NF-kappaB in minutes, NF-kappaB activation induced by anticancer drugs usually occurred more than 1hr after stimulation
Liacini et al., Matrix Biol 2002 (Osteoarthritis, Hip) : Inhibition of interleukin-1 stimulated MAP kinases, activating protein-1 (AP-1) and nuclear factor kappa B (NF-kappa B) transcription factors down-regulates matrix metalloproteinase gene expression in articular chondrocytes ... These results suggest the involvement of MAPKs, AP-1 and NF-kappa B transcription factors in the IL-1 induction of MMPs in chondrocytes
Cho et al., Nitric Oxide 2002 (MAP Kinase Signaling System) : These results demonstrated that bovine type I collagen induces iNOS in serum stimulated murine macrophages through JunB/AP-1 and NF-kappa B activation and that activation of ERK1/2 plays an essential role in JunB/AP-1 activation ... These results demonstrated that bovine type I collagen induces iNOS in serum stimulated murine macrophages through JunB/AP-1 and NF-kappa B activation and that activation of ERK1/2 plays an essential role in JunB/AP-1 activation
Pohlers et al., Arthritis Res 2002 (Arthritis, Experimental...) : Differential clinical effects at the onset of AA were paralleled by a differential influence of the mAbs on T-cell functions, i.e. in comparison with OX35 and W3/25, the ` accelerating ' mAb RIB5/2 failed to increase the delayed-type hypersentivity ( DTH ) to Mycobacterium tuberculosis, increased the in vitro tumor necrosis factor (TNF)-alpha secretion, and more strongly induced NF-kappaB binding activity after anti-CD4 preincubation and subsequent TCR/CD3-stimulation
Hata et al., Am J Physiol Gastrointest Liver Physiol 2002 (Inflammatory Bowel Diseases) : IL-17 induced NF-kappaB activation within 45 min after stimulation
Hassoun et al., Am J Physiol Gastrointest Liver Physiol 2002 (Intestinal Obstruction...) : Rats subjected to I/R exhibited markedly depressed intestinal transit, histological evidence of severe injury to the ileum, increased myeloperoxidase activity in ileal cytoplasmic extracts, and biphasic activation of NF-kappaB in ileal nuclear extracts
Liu et al., Ann Clin Lab Sci 2002 (Lung Diseases...) : Growth hormone increases lung NF-kappaB activation and lung microvascular injury induced by lipopolysaccharide in rats ... The purpose of this study was to examine the effects of growth hormone (GH) on nuclear factor kappa B (NF-kappaB) activation and organ injury induced by lipopolysaccharide (LPS) in rats
Hua et al., J Neuroimmunol 2002 : Electrophoretic mobility shift assay ( EMSA ) showed that IL-1 induced NF-kappaB and AP-1 DNA complex formation in astrocytes, and that SB203580 inhibited AP-1 complex formation ... Electrophoretic mobility shift assay ( EMSA ) showed that IL-1 induced NF-kappaB and AP-1 DNA complex formation in astrocytes, and that SB203580 inhibited AP-1 complex formation
Yan et al., Biol Reprod 2002 : In this study, we investigated the possible role of the nuclear factor kappa B (NFkappaB) in IL-1beta signaling, leading to the expression of COX-2 in human amnion cell culture ... These results suggest that NFkappaB is involved in the IL-1beta induced COX-2 expression in the mesenchymal cells of human amnion
Leonardi et al., Endocrinology 2002 : Dominant negative TNF receptor associated factor 2 (TRAF2) inhibited activation of NF-kappa B , which was also inhibited in embryonic fibroblasts derived from TRAF2 ( -/- ) mice, respect to their normal counterpart
Huber et al., Digestion 2002 : Western blot analysis revealed that TNF-alpha caused degradation of I kappa B alpha within 10 min. EMSA demonstrated that TNF-alpha led to increased DNA binding activity of NF kappa B and that proteasome inhibitors counteracted NF kappa B activation
Lukita-Atmadja et al., Shock 2002 (Endotoxemia...) : Curcuminoids, derived from the plant Curcuma domestica Val., have been shown to be free radical scavengers that suppress the production of superoxide by macrophages and potent anti-inflammatory agents that inhibit the lipopolysacharide (LPS) induced production of tumor necrosis factor alpha (TNFalpha) , interleukin (IL)-1beta, and the activation of nuclear factor (NF)-kappaB in human monocytic derived cells ... Curcuminoids, derived from the plant Curcuma domestica Val., have been shown to be free radical scavengers that suppress the production of superoxide by macrophages and potent anti-inflammatory agents that inhibit the lipopolysacharide (LPS) induced production of tumor necrosis factor alpha (TNFalpha), interleukin (IL)-1beta , and the activation of nuclear factor (NF)-kappaB in human monocytic derived cells ... Curcuminoids, derived from the plant Curcuma domestica Val., have been shown to be free radical scavengers that suppress the production of superoxide by macrophages and potent anti-inflammatory agents that inhibit the lipopolysacharide (LPS) induced production of tumor necrosis factor alpha (TNFalpha), interleukin (IL)-1beta , and the activation of nuclear factor (NF)-kappaB in human monocytic derived cells
Murr et al., J Gastrointest Surg 2002 : Elastase induced overexpression of TNF messengerRNA and activation of NF-kappa B was attenuated by Gd. Pancreatic elastase induces a pattern of liver injury similar to that seen during acute pancreatitis by activating cytokine production and gene expression within Kupffer cells via NF-kappa B
Kamata et al., FEBS Lett 2002 : Electrophoretic mobility shift assay revealed that H2O2 enhanced TNF induced NF-kappaB activation
O'Sullivan et al., J Immunol 2002 : However, NF-kappaB activation induced by TNF-alpha could be sustained by blocking autocrine IL-10
Sugita et al., J Immunol 2002 : Induction of macrophage-inflammatory protein-3alpha gene expression by TNF dependent NF-kappaB activation ... Induction of macrophage-inflammatory protein-3alpha gene expression by TNF dependent NF-kappaB activation ... MIP-3alpha transcription was stimulated by TNF, and this stimulation was inhibited by an NF-kappaB inhibitor, I-kappaBalpha superrepressor ... These results clearly demonstrate that MIP-3alpha gene expression is dependent on NF-kappaB activity in vitro, and indicate that the TNFR1 mediated TNF signaling cascade that leads to NF-kappaB activation plays an essential role in MIP-3alpha expression in the murine liver injury model
Haehnel et al., J Immunol 2002 : In contrast to murine TLR2, expression of human TLR2 in monocytes/macrophages is not induced by the proinflammatory stimuli LPS or macrophage activating lipopeptide-2, and reporter activity of the promoter was not enhanced by stimuli induced NF-kappaB activation in THP-1 or MonoMac-6 cells
Mariner et al., J Immunol 2002 : These findings suggest a role for both NF-kappaB and IRF-4 in the transcriptional regulation of IL-15Ralpha by HTLV-I Tax
Herndon et al., Clin Immunol 2002 (Arthritis, Rheumatoid...) : Since NF-kappaB contributes to the transcriptional regulation of the IL-2 promoter, the effect of the forced replenishment of p65 on IL-2 transcription was tested
Chapman et al., Biochem J 2002 (Neoplasms) : Furthermore, NF-kappaB can induce c-Myc gene expression, suggesting that the activities of these factors are functionally linked
Kayal et al., Mol Microbiol 2002 : Although a delayed kinetics of LLO dependent NF-kappaB activation suggests an autocrine or paracrine IL-1 dependent pathway, we found that LLO dependent NF-kappaB activation did not require the IL-1 protein synthesis nor the interaction with the IL-1RI specific receptor ... Herein, we demonstrated that LLO dependent NF-kappaB activation requires the activation of the IkappaB kinase beta (IKKbeta) subunit of IKK complex to phosphorylate and degrade cytoplasmic IkappaBalpha , a natural inhibitor of NF-kappaB
Sawano et al., Kidney Int 2002 : 15d-PGJ2 inhibited the IL-1beta induced increase in binding activities of nuclear proteins to consensus AP-1 site and AP-1-like site of COX-2 promoter but not of NF-kappaB
Wolf et al., Kidney Int 2002 : In AT1 or AT2 receptor transfected cells, but not untransfected COS7 cells, 10-7 mol/L Ang II induced NF-kappaB DNA binding in vitro, as detected by electrophoretic shift assays and in vivo transactivation of a reporter gene construct ... Ang II-induced activation of NF-kappaB was associated with degradation of IkappaB-alpha in all studied cell lines ... Our results clearly demonstrate in various cell lines that Ang II induces NF-kappaB activation through AT2 receptors
Lal et al., Kidney Int 2002 : NF-kappaB was dose-dependently activated by AGE ... Antioxidants, vitamin E and nitecapone, prevented AGE dependent NF-kappaB activation and normalized PKC activity and associated TGF-beta1 transcription
Djavaheri-Mergny et al., Mol Cell Biochem 2002 : Moreover, we show that lipid peroxidation is involved in GGT dependent NF-kappaB activation since vitamin E, which completely inhibits GGT induced generation of lipid peroxides, prevents the GGT dependent NF-kappaB activation ... Moreover, we show that lipid peroxidation is involved in GGT dependent NF-kappaB activation since vitamin E, which completely inhibits GGT induced generation of lipid peroxides, prevents the GGT dependent NF-kappaB activation ... This finding suggests that GGT mediated NF-kappaB activation plays a role in the control of apoptosis in U937 cells
Hattori et al., Cardiovasc Res 2002 : Both statins slightly modulated IL-1/IFN induced NF-kappaB activation, which was not associated with their effect on NO production
Altavilla et al., Cardiovasc Res 2002 (Shock, Septic) : Moreover we studied in LPS stimulated ( 50 microg ml(-1) ) peritoneal macrophages ( Mphi ), NF-kappaB activation, cytoplasmic IkappaB-alpha degradation, the message for TNF-alpha, and TNF-alpha and MAL levels ... In addition, in vitro LPS stimulation ( 50 microg ml(-1) ) significantly induced NF-kappaB activation and cytoplasmic IkappaBalpha degradation in Mphi, enhanced TNF-alpha mRNA levels and increased Mphi TNF-alpha and MAL ... Furthermore ` in vitro ' IRFI 042 ( 50 microM ) significantly inhibited activation of NF-kappaB through inhibition of IkappaBalpha degradation, reduced the amount of TNF-alpha mRNA, decreased LPS induced TNF-alpha release and blunted lipid peroxidation ( MAL ) in LPS stimulated Mphi
Chong et al., J Biol Chem 2002 (MAP Kinase Signaling System) : A further analysis revealed that SOCS-1 deficiency results in augmented TNF signaling via the p38 mitogen activated protein kinase pathway but not NFkappaB or c-Jun N-terminal kinase pathways
Heckman et al., Oncogene 2002 (Lymphoma...) : NF-kappaB activates Bcl-2 expression in t ( 14 ; 18 ) lymphoma cells ... Conflicting evidence exists on the effects of NF-kappaB expression on Bcl-2 levels in different cell types
Delhalle et al., Oncogene 2002 (Adenocarcinoma) : Therefore, MnSOD expression is induced by NF-kappaB in epithelial cancer cells in response to TNF-alpha , and is at least partially responsible for their resistance to TNF-alpha induced apoptosis, presumably through the clearance of death inducing ROS
Patel et al., J Interferon Cytokine Res 2002 (Melanoma) : Treatment of melanoma cells with capsaicin inhibited activation of constitutive and IL-1beta induced NF-kappaB , but not AP-1, leading to inhibition of IL-8 expression ... These results demonstrate that constitutive and induced NF-kappaB activation regulates IL-8 expression in melanoma cells ... Downregulation of constitutive and induced NF-kappaB activation in malignant melanoma cells leads to inhibition of IL-8 production and in vitro cell proliferation
Hagood et al., Am J Respir Cell Mol Biol 2002 : IL-1beta induces DNA binding of both nuclear factor kappaB (NF-kappaB) and CAATT-enhancer binding protein (C/EBP), and activation of p38 mitogen activated protein kinase in both subpopulations ... IL-1beta induces DNA binding of both nuclear factor kappaB (NF-kappaB) and CAATT-enhancer binding protein (C/EBP), and activation of p38 mitogen activated protein kinase in both subpopulations
Ozaki et al., FASEB J 2002 : In a model of mouse hepatic ischemia/reperfusion ( I/R ), recombinant adenoviral overexpression of ref-1 resulted in suppression of reperfusion stimulated oxidative stress, NF-kB induction, apoptosis, and acute injury, whereas down-regulation of endogenous ref-1 by adenoviral expression of antisense ref-1 led to an increase in these reperfusion induced parameters
Viedt et al., J Am Soc Nephrol 2002 (Nephritis) : Using electrophoretic gel mobility shift assay, we observed that MCP-1 stimulated binding activity of NF-kappaB ... In the present experiments, NF-kappaB and AP-1 were involved in the MCP-1 mediated induction of IL-6 , as demonstrated by cis element double stranded ( decoy ) oligonucleotides ( ODN ) ... In contrast to IL-6 release, MCP-1 induced ICAM-1 expression was predominantly dependent on NF-kappaB activation
Islam et al., Mol Endocrinol 2002 : A role for endogenous NF-kappaB in cAMP and IL-1 regulation of SP-A was suggested by findings that dominant negative forms of inhibitor of kappaB reduced binding of type II cell nuclear proteins to TBE and inhibited SP-A expression
Schoemaker et al., J Hepatol 2002 (Hepatitis) : cIAP2 is induced by cytokines in an NF-kappaB dependent manner and overexpression of cIAP2 inhibits apoptosis
Tanaka et al., Mol Cell 2002 : In these clones, E2F1 inhibited NF-kappaB activity by binding to its subunit p65 in competition with a heterodimeric partner p50 ... In these clones, E2F1 inhibited NF-kappaB activity by binding to its subunit p65 in competition with a heterodimeric partner p50 ... In addition to overexpressed E2F1, endogenous E2F1 released from Rb was also found to inhibit NF-kappaB activity in a cell cycle dependent manner by using E2F1 ( +/+ ) and E2F1 ( -/- ) murine embryonic fibroblasts
Takehana et al., Biochem Biophys Res Commun 2002 : Despite having a strong inhibitory effect on the NF-kappaB dependent transcriptional activation, APC0576 does not inhibit the IL-1 induced DNA binding of NF-kappaB , degradation of I-kappaB-alpha, or phosphorylation of RelA ( p65 ) ... Despite having a strong inhibitory effect on the NF-kappaB dependent transcriptional activation, APC0576 does not inhibit the IL-1 induced DNA binding of NF-kappaB , degradation of I-kappaB-alpha, or phosphorylation of RelA ( p65 )
Carballo et al., Mol Genet Metab 2002 : Additionally, PD144795 did not block NF-kappa B nuclear translocation induced by LPS
Günthert et al., Biochem Biophys Res Commun 2002 (Adenocarcinoma...) : Since LHRH activates nucleus factor kappa B (NF kappa B) and protects ovarian cancer cells from doxorubicin induced apoptosis and JunD is shown to decrease cell cycle and cell proliferation, we propose that JunD activated by LHRH acts as a modulator of cell proliferation and cooperates with the anti-apoptotic and anti-mitogenic functions of LHRH
Jijon et al., Am J Physiol Cell Physiol 2002 : Furthermore, AP-1 and NF-kappaB DNA binding was not affected by ERK and p38 inhibition ... Furthermore, AP-1 and NF-kappaB DNA binding was not affected by ERK and p38 inhibition
Kilpatrick et al., Am J Physiol Cell Physiol 2002 : Inhibition of either PKC-delta or PI 3-kinase attenuated TNF-alpha mediated activation of the antiapoptotic transcription factor NFkappaB ... Inhibition of either PKC-delta or PI 3-kinase attenuated TNF-alpha mediated activation of the antiapoptotic transcription factor NFkappaB
Kuang et al., Am J Physiol Cell Physiol 2002 : NF-kappaB induced by IL-1beta inhibits elastin transcription and myofibroblast phenotype ... NF-kappaB induced by IL-1beta inhibits elastin transcription and myofibroblast phenotype ... These results indicate that IL-1beta activates the nuclear localization of NF-kappaB that subsequently interacts with Sp1 to downregulate elastin transcription and expression of the myofibroblast phenotype
Russo et al., Am J Physiol Cell Physiol 2002 : Differential requirement for NF-kappaB inducing kinase in the induction of NF-kappaB by IL-1beta, TNF-alpha , and Fas ... Differential requirement for NF-kappaB inducing kinase in the induction of NF-kappaB by IL-1beta, TNF-alpha, and Fas ... Differential requirement for NF-kappaB inducing kinase in the induction of NF-kappaB by IL-1beta , TNF-alpha, and Fas ... Interleukin (IL)-1beta- and tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activation and kappaB dependent gene expression are inhibited in HeLa cells but not in Ad5dnNIK infected HT-29 cells
Angkeow et al., Cell Death Differ 2002 : Forced cytoplasmic overexpression of ref-1 suppressed H/R induced oxidative stress ( H ( 2 ) O ( 2 ) production ), NF-kappaB activation, and apoptosis, and also mitigated rac1 regulated H ( 2 ) O ( 2 ) production and NF-kappaB transcriptional activity
Garrouste et al., Cell Death Differ 2002 : Correspondingly, IGF-I markedly enhanced the TNF- and IFN/TNF induced NF-kappaB dependent interleukin-8 production ... Correspondingly, IGF-I markedly enhanced the TNF- and IFN/TNF induced NF-kappaB dependent interleukin-8 production
Aikawa et al., Inflamm Res 2002 (Leukemia, Monocytic, Acute) : EMSA study showed that T-614 prevented TNF-alpha as well as LPS stimulated activation of NF-kappaB , and Western blot analysis proved that T-614 did not affect degradation of IkappaBalpha protein
Mukhopadhyay et al., J Biol Chem 2002 : Ectopic expression of protein-tyrosine kinase Bcr-Abl suppresses tumor necrosis factor (TNF) induced NF-kappa B activation and IkappaBalpha phosphorylation ... We used a bcr-abl-deficient human megakaryocytic leukemia cell line MO7E and an isogenic MBA cell line stably transfected with bcr-abl. Electrophoretic mobility shift assay revealed that TNF activated the nuclear transcription factor NF-kappaB in MO7E cells but not in MBA cells ... The impaired NF-kappaB activation in Bcr-Abl expressing cells was not due to absence of the NF-kappaB proteins p65, p50, or p100 or of IkappaBalpha or IkappaBbeta ... The impaired NF-kappaB activation in Bcr-Abl expressing cells was not due to absence of the NF-kappaB proteins p65, p50, or p100 or of IkappaBalpha or IkappaBbeta ... The suppression of TNF induced NF-kappaB activation by Bcr-Abl was not restricted to MBA cells, because ectopic expression of Bcr-Abl in human acute myeloid leukemia HL-60 cells also blocked TNF induced NF-kappaB activation ... The suppression of TNF induced NF-kappaB activation by Bcr-Abl was not restricted to MBA cells, because ectopic expression of Bcr-Abl in human acute myeloid leukemia HL-60 cells also blocked TNF induced NF-kappaB activation ... The suppression of TNF induced NF-kappaB activation by Bcr-Abl was not restricted to MBA cells, because ectopic expression of Bcr-Abl in human acute myeloid leukemia HL-60 cells also blocked TNF induced NF-kappaB activation
Naito et al., Proc Natl Acad Sci U S A 2002 (Ectodermal Dysplasia) : Furthermore, TRAF6 is essential for the XEDAR mediated NFkappaB activation
Andoh et al., Int J Mol Med 2002 (Acute Disease...) : Furthermore, somatostatin suppressed TNF-alpha induced NF-kappaB activation ... Furthermore, somatostatin suppressed TNF-alpha induced NF-kappaB activation
You et al., Biochem Biophys Res Commun 2002 : Transient expression and electrophoretic mobility shift assays with NF-kappaB binding sites revealed that NP reduced the levels of the LPS induced NF-kappaB transcription factor complex
Mazzeo et al., Cytokine 2002 : NAC did not inhibit p40 or p35 mRNA expression in dendritic cells or THP-1 cells, or NF-kappa B activation in THP-1 cells
Adams et al., Cardiovasc Res 2002 (Heart Failure) : Induction of iNOS expression in skeletal muscle by IL-1beta and NFkappaB activation : an in vitro and in vivo study
Ramana et al., J Biol Chem 2002 : Inhibition of AR in VSMC also prevented the activation of NF-kappa B by basic fibroblast growth factor (bFGF) , angiotensin-II (Ang-II), and platelet derived growth factor ( PDGF-AB ) ... Inhibition of AR in VSMC also prevented the activation of NF-kappa B by basic fibroblast growth factor (bFGF), angiotensin-II (Ang-II) , and platelet derived growth factor ( PDGF-AB )
Zimmerman et al., Am J Physiol Heart Circ Physiol 2002 : The purposes of this study were to examine the effects of IL-11 on human vascular smooth muscle cell ( VSMC ) proliferation and NF-kappa B activity
Philpott et al., Cell Microbiol 2002 (Disease Models, Animal...) : Six isolates were shown to harbour a complete cag PAI and to induce NF- kappa B activation and IL-8 secretion in AGS cells
Schwabe et al., Am J Physiol Gastrointest Liver Physiol 2002 : Role of glycogen synthase kinase-3 in TNF-alpha induced NF-kappaB activation and apoptosis in hepatocytes ... In this study, we determined the role of GSK-3 in TNF-alpha induced NF-kappaB activation and cell death in primary hepatocytes
Che et al., Circ Res 2002 : Insulin-like growth factor-1 enhances inflammatory responses in endothelial cells : role of Gab1 and MEKK3 in TNF-alpha induced c-Jun and NF-kappaB activation and adhesion molecule expression ... Insulin-like growth factor-1 enhances inflammatory responses in endothelial cells : role of Gab1 and MEKK3 in TNF-alpha induced c-Jun and NF-kappaB activation and adhesion molecule expression ... Insulin-like growth factor-1 enhances inflammatory responses in endothelial cells : role of Gab1 and MEKK3 in TNF-alpha induced c-Jun and NF-kappaB activation and adhesion molecule expression ... Because Gab1 is involved in both c-Jun and NF-kappaB activation by TNF-alpha , we focused on Gab1 dependent signaling ... Because Gab1 is involved in both c-Jun and NF-kappaB activation by TNF-alpha, we focused on Gab1 dependent signaling ... Gab1 associated with MEKK3, and a catalytically inactive form of MEKK3 inhibited TNF-alpha induced c-Jun and NF-kappaB transcriptional activation, suggesting a critical role for Gab1 and MEKK3 in TNF-alpha signaling ... Gab1 associated with MEKK3, and a catalytically inactive form of MEKK3 inhibited TNF-alpha induced c-Jun and NF-kappaB transcriptional activation, suggesting a critical role for Gab1 and MEKK3 in TNF-alpha signaling ... Gab1 associated with MEKK3, and a catalytically inactive form of MEKK3 inhibited TNF-alpha induced c-Jun and NF-kappaB transcriptional activation, suggesting a critical role for Gab1 and MEKK3 in TNF-alpha signaling
Shou et al., J Neurochem 2002 : NF-kappaB mediated up-regulation of Bcl-X ( S ) and Bax contributes to cytochrome c release in cyanide induced apoptosis ... NF-kappaB mediated up-regulation of Bcl-X ( S ) and Bax contributes to cytochrome c release in cyanide induced apoptosis ... NF-kappaB , a redox-sensitive transcription factor activated after cyanide treatment, is responsible for the up-regulation of Bcl-X ( S ) and Bax ... NF-kappaB , a redox-sensitive transcription factor activated after cyanide treatment, is responsible for the up-regulation of Bcl-X ( S ) and Bax ... It was concluded that NF-kappaB mediated up-regulation of Bcl-X ( S ) and Bax is involved in regulating cytochrome c release in cyanide induced apoptosis ... It was concluded that NF-kappaB mediated up-regulation of Bcl-X ( S ) and Bax is involved in regulating cytochrome c release in cyanide induced apoptosis
Kang et al., J Pharmacol Exp Ther 2002 : To further investigate the mechanism responsible for the inhibition of iNOS gene expression by silymarin, we examined the effect of silymarin on LPS induced nuclear factor-kappaB (NF-kappaB)/Rel activation, which regulates various genes involved in immune and inflammatory response ... In RAW 264.7 cells, the LPS induced DNA binding activity of NF-kappaB/Rel was significantly inhibited by silymarin, and this effect was mediated through the inhibition of the degradation of inhibitory factor-kappaB ... Silymarin also inhibited tumor necrosis factor-alpha induced NF-kappaB/Rel activation, whereas okadaic acid induced NF-kappaB/Rel activation was not affected ... Collectively, these results suggest that silymarin inhibits nitric oxide production and iNOS gene expression by inhibiting NF-kappaB/Rel activation
Luckey et al., J Pharmacol Exp Ther 2002 (Adenoviridae Infections) : The role of nuclear factor-kappaB (NF-kappaB) in IL-6 induction was elucidated using Kupffer cells transduced in vitro with a recombinant adenovirus containing a IkappaBalpha super-repressor resistant to phosphorylation and degradation ( Ad5IkappaB )
Tsi et al., Mol Pharmacol 2002 : ATA not only inhibited nuclear factor-kappaB (NF-kappaB) activation through impairment of the targeting and degradation of IkappaBs but also reduced LPS induced activator protein-1 (AP-1) activation
Umeda et al., Int Arch Allergy Immunol 2002 : We examined whether or not theophylline inhibits tumor necrosis factor (TNF)-alpha induced activation of the nuclear transcription factor NF-kappaB , a factor that is essential for the expression of proinflammatory cytokines, in human monocytic U-937 cells, a T cell line ( Jurkat ) and peripheral blood mononuclear cells ( PBMC ) ... The inhibitory effect of theophylline on TNF-alpha induced NF-kappaB activation was evaluated by Western blotting, flow cytometry and chloramphenicol acetyltransferase (CAT) assaying
Wong et al., Clin Exp Immunol 2002 (MAP Kinase Signaling System) : Using EMSA, both MG-132 and NaSal were found to suppress the TNF induced NF-kappaB activation in eosinophils
Youn et al., Immunol Lett 2002 (Arthritis, Rheumatoid...) : This TNF-alpha induced proliferation of synoviocytes obtained from RA patients coincided with down-regulation of TNFR1 and up-regulation of TNFR2 and TRAF1-6, as well as NF-kappaB activation ... This TNF-alpha induced proliferation of synoviocytes obtained from RA patients coincided with down-regulation of TNFR1 and up-regulation of TNFR2 and TRAF1-6 , as well as NF-kappaB activation
Viedt et al., Arterioscler Thromb Vasc Biol 2002 (Inflammation) : MCP-1 stimulated the binding activity of NF-kappaB and of activator protein-1 (AP-1) ... As demonstrated by cis element double stranded ( decoy ) oligodeoxynucleotides, NF-kappaB was involved in IL-6 release by MCP-1, whereas proliferation was dependent on AP-1 ... The results clearly demonstrate that MCP-1 induces differential activation of NF-kappaB and AP-1 in VSMCs
Molina-Holgado et al., J Neurochem 2002 (Cardiovirus Infections) : The proteasome inhibitor MG-132 blocked TMEV induced nitrite accumulation, NOS-2 mRNA expression and phospho-IkappaBalpha degradation, suggesting NF-kappaB dependent NOS-2 expression ... Therefore, the accumulation of IkappaBalpha might impair the translocation of the NF-kappaB to the nucleus, mediating the inhibition of NF-kappaB activity
Kim et al., J Neurochem 2002 : IFNbeta induced IkappaBalpha degradation and NF-kappaB ( p65 : p50 ) DNA binding ... PD98059 did not inhibit NF-kappaB binding, demonstrating that ERK was not responsible for NF-kappaB activation
Zingarelli et al., Shock 2002 (Shock, Septic) : These events were preceded by degradation of inhibitors kappaBalpha ( IkappaBalpha ) and kappaBI ( IkappaBbeta ), and activation of nuclear factor-kappaB (NF-kappaB) in the lung ... These events were preceded by lesser degradation of IkappaBalpha and reduced NF-kappaB activation in the lung ... Our data suggests that iNOS is not required for the development of endotoxin tolerance, and that other signal transduction pathways, rather than NF-kappaB , may regulate induction of endotoxin tolerance in the absence of iNOS
Tsuyuki et al., Biochim Biophys Acta 2002 : As we had found previously that thapsigargin, an endomembrane Ca2+-ATPase inhibitor, induces production of intracellular platelet activating factor (PAF) [ Br. J. Pharmacol. 116 ( 1995 ) 2141 ], we decided to investigate the possible roles of intracellular PAF in nuclear factor (NF)-kappaB activation of thapsigargin stimulated rat peritoneal macrophages ... Lipopolysaccharide (LPS) induced activation of NF-kappaB was also suppressed by these drugs
Saijo et al., J Exp Med 2002 : Using mice deficient for protein kinase C beta (PKCbeta) we show an essential role of PKCbeta in the phosphorylation of IKKalpha and the subsequent activation of NF-kappaB in B cells ... Using mice deficient for protein kinase C beta (PKCbeta) we show an essential role of PKCbeta in the phosphorylation of IKKalpha and the subsequent activation of NF-kappaB in B cells ... Lack of IKKalpha phosphorylation and defective NF-kappaB induction in the absence of PKCbeta explains the similarity in immunodeficiencies caused by PKCbeta or IKKalpha ablation in B cells
Wang et al., Endocrinology 2002 : The gonadotropin also induced granulosa cell p65 subunit containing NFkappaB translocation from cytoplasm to nucleus and increased NFkappaB-DNA binding activity
Pasparakis et al., Nature 2002 (Inflammation...) : IKK2 and NEMO are necessary for NF-kappa B activation through pro-inflammatory signals ... IKK2 and NEMO are necessary for NF-kappa B activation through pro-inflammatory signals ... IKK2 deficiency inhibits NF-kappa B activation, but does not lead to cell-autonomous hyperproliferation or impaired differentiation of keratinocytes ... Our results suggest that the critical function of IKK2 mediated NF-kappa B activity in epidermal keratinocytes is to regulate mechanisms that maintain the immune homeostasis of the skin
Takeuchi et al., J Immunol 2002 : TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhanced the NF-kappaB activation in response to a synthetic lipopeptide ... TLR1 interacts with TLR2 and coexpression of TLR1 and TLR2 enhanced the NF-kappaB activation in response to a synthetic lipopeptide
Valéro et al., J Immunol 2002 (Autoimmune Diseases...) : Upon TNF induced activation of NZB thymocytes, nuclear translocation and DNA binding of RelA- and RelB dependent NF-kappaB heterodimers are significantly reduced
Fotin-Mleczek et al., J Cell Sci 2002 (Rhabdomyosarcoma) : When both TNF-receptors were stimulated simultaneously, TNF-R1 induced NF-kappaB activation remained unaffected but TNF-R1 induced apoptosis was still significantly enhanced ... When both TNF-receptors were stimulated simultaneously, TNF-R1 induced NF-kappaB activation remained unaffected but TNF-R1 induced apoptosis was still significantly enhanced
Ribardo et al., Microb Pathog 2002 (Calcium Signaling) : Taken together, a link between Act induced calcium release, regulation of downstream kinase cascades and Ref-1 , and activation of NF-kappa B leading to PGE ( 2 ) and TNF alpha production was established
Ohkita et al., Jpn J Pharmacol 2002 : BAY 11-7082, an inhibitor of nuclear factor-kappaB (NF-kappaB), which prevents a step of the phosphorylation of inhibitory protein IkappaB bound to NF-kappaB, suppressed basal and tumor necrosis factor (TNF)-alpha induced prepro endothelin (ET)-1 mRNA expression and NF-kappaB activation in cultured vascular endothelial cells
Bannerman et al., Biochem Biophys Res Commun 2002 : TIRAP mediates endotoxin induced NF-kappaB activation and apoptosis in endothelial cells ... Recently, a new signaling molecule, TIRAP, has been identified that mediates LPS induced NF-kappaB signaling in monocytes and macrophages ... Using a TIRAP dominant negative construct, we have identified a role for TIRAP in mediating LPS induced NF-kappaB activation and apoptosis in human endothelial cells ... Using a TIRAP dominant negative construct, we have identified a role for TIRAP in mediating LPS induced NF-kappaB activation and apoptosis in human endothelial cells
Taggart et al., J Biol Chem 2002 : In this report we have shown that SLPI prevents LPS induced NF-kappaB activation by inhibiting degradation of IkappaBalpha without affecting the LPS induced phosphorylation and ubiquitination of IkappaBalpha
Kim et al., Oncogene 2002 (Cell Transformation, Neoplastic) : Although the abundance of the inhibitor protein IkappaBbeta was higher in 267B1/Ki-ras cells than in 267B1 cells, an electrophoretic mobility-shift assay suggested that IkappaBalpha is responsible for the activation of NF-kappaB in the former cells ... The expression of dominant negative mutants of either NIK ( NF-kappaB inducing kinase ) or IKKbeta ( IkappaB kinase beta ) inhibited the activity of NF-kappaB in 267B1/Ki-ras cells
Kim et al., J Environ Pathol Toxicol Oncol 2002 (Adenocarcinoma...) : We studied the role of NF-kappaB on expression of COX-2 in H. pylori stimulated gastric cancer cell lines by using antioxidants, glutathione ( GSH ), and N-acetylcysteine (NAC) as well as an NF-kappaB inhibitor, pyrrolidine dithiocarbamate ( PDTC ) ... Gastric adenocarcinoma cell lines derived from Caucasian ( AGS ) cells and Korean ( SNU-484 ) cells were used to study the role of NF-kappaB on COX-2 expression by H. pylori ... In conclusion, oxidant-sensitive transcription factor NF-kappaB may play a novel role in expression of COX-2 by H. pylori stimulation in gastric cancer cells
Chun et al., J Environ Pathol Toxicol Oncol 2002 : Effects of yakuchinone A and yakuchinone B on the phorbol ester induced expression of COX-2 and iNOS and activation of NF-kappaB in mouse skin ... Effects of yakuchinone A and yakuchinone B on the phorbol ester induced expression of COX-2 and iNOS and activation of NF-kappaB in mouse skin
Shah et al., J Gastrointest Surg 2001 (Adenocarcinoma...) : NF-kappaB dependent gene expression, however, was ultimately suppressed by Ly294002, suggesting that PI3k dependent activation of NF-kappaB is IkappaBalpha independent
Wayman et al., FASEB J 2002 (Myocardial Infarction...) : The mechanisms of the cardioprotective effects of 15D-PGJ2 may include 1 ) activation of PPAR-alpha, 2 ) activation of PPAR-gamma , 3 ) expression of HO-1, and 4 ) inhibition of the activation of NF-kappaB in the ischemic reperfused heart ... Inhibition by 15D-PGJ2 of the activation of NF-kappaB in turn results in a reduction of the 1 ) expression of inducible nitric oxide synthase and the nitration of proteins by peroxynitrite, 2 ) formation of the chemokine MCP-1, and 3 ) expression of the adhesion molecule ICAM-1
Chen et al., J Dermatol Sci 2002 : Propionibacterium acnes induced IL-8 production may be mediated by NF-kappaB activation in human monocytes
Fujita et al., Mol Cell Endocrinol 2002 (Hyperglycemia...) : Angiotensin II (Ang II) significantly enhanced DNA binding activity of NF-kappaB under HG ( 25.6 mM ) conditions with an increase in p65 subunit of NF-kappaB, and did it slightly under normal glucose ( NG ; 5.6 mM ) conditions
Qian et al., Proc Natl Acad Sci U S A 2002 : Overexpression of the NF kappa B activator 1 (Act1) leads to the activation of both NF kappa B and Jun kinase in epithelial cell lines ... Furthermore, transfection of Act1 into C33A cervical epithelial cells, which do not express it, renders these cells sensitive to CD40 ligand induced NF kappa B activation and protects them from CD40 ligand induced apoptosis
Gozal et al., Am J Respir Cell Mol Biol 2002 : RAW 264.7 macrophages exhibited enhanced TNF-alpha production and NF-kappaB activation in response to silica, whereas IC-21 macrophages did not produce TNF-alpha in response to silica and did not induce NF-kappaB nuclear binding ... In addition, TNF-alpha and NF-kappaB activation, but not apoptosis, were induced by lipopolysaccharide (LPS) in both cell lines, and NF-kappaB inhibition reduced LPS induced TNF-alpha release ... In addition, TNF-alpha and NF-kappaB activation, but not apoptosis, were induced by lipopolysaccharide (LPS) in both cell lines, and NF-kappaB inhibition reduced LPS induced TNF-alpha release ... These data suggest that TNF-alpha induction is dependent on NF-kappaB activation in both cell lines
Park et al., Nutr Cancer 2001 : To clarify the transcriptional regulatory pathway, we investigated the role of nuclear factor-kappa B (NF-kappa B) in the expression of VCAM-1 by linoleic acid in HMEC-1 ... The role of NF-kappa B in linoleic acid induced VCAM-1 expression was confirmed by functional promoter studies in HMEC-1 transfected with reporter constructs of the VCAM-1 promoter with or without mutated NF-kappa B binding site
Korus et al., Oncogene 2002 (MAP Kinase Signaling System) : p38 MAPK mediated activation of NF-kappaB by the RhoGEF domain of Bcr ... NF-kappaB activation by Bcr is not mediated by nuclear translocation, but rather by p38 mitogen activated protein kinase ( MAPK ) -dependent modification of the RelA/p65 transactivation domain
Morel et al., J Biol Chem 2002 (Arthritis, Rheumatoid...) : In an alternate pathway, inhibition of IL-1 receptor associated kinase-1 (IRAK) with AS ODN to IRAK reduced IL-18 induced expression of nuclear factor kappaB (NFkappaB) ... In an alternate pathway, inhibition of IL-1 receptor associated kinase-1 (IRAK) with AS ODN to IRAK reduced IL-18 induced expression of nuclear factor kappaB (NFkappaB)
Wang et al., J Biol Chem 2002 (Inflammation) : Furthermore, constitutive activation of PPAR-gamma resulted in simultaneous repression of AP-1 and NF-kappaB activity, which suggests that PPAR-gamma may reduce pro-inflammatory phenotypes via, at least in part, suppression of the AP-1 and NF-kappaB pathways
Tamura et al., J Clin Endocrinol Metab 2002 : COX-2 mRNA, protein, and PGE ( 2 ) levels in IL-1beta treated ESC were decreased by a PKA inhibitor, a nuclear factor ( NF-kappaB ) inhibitor, and an ERK1/2 inhibitor, but not by a p38 MAPK inhibitor or a PKC inhibitor, suggesting the possible involvement of PKA, NF-kappaB , and/or the ERK1/2 signaling pathway ( s ) in IL-1beta mediated COX-2 gene induction in ESC
Nickoloff et al., Cell Death Differ 2002 : Jagged-1 mediated activation of notch signaling induces complete maturation of human keratinocytes through NF-kappaB and PPARgamma ... In KC monolayers, addition of JAG-1 peptide induced IKKalpha mediated NF-kappaB activation, as well as increased PPARgamma expression
Takayanagi et al., Arthritis Res 2002 (Arthritis...) : This cytokine induces rapid degradation of the RANK ( receptor activator of nuclear factor kappaB ) adapter protein TRAF6 ( TNF-receptor associated factor 6 ), resulting in strong inhibition of the RANKL induced activation of NF-kappaB and JNK ( c-Jun N-terminal kinase )
Kawakami et al., Med Electron Microsc 2002 (Arthritis, Rheumatoid...) : Human T-lymphotropic virus type I ( HTL V-I ) is one of the pathogenic organisms for RA and SS, and we demonstrated that HTL V-I tax stimulates NF-kappa B nuclear translocation, inhibiting apoptotic cell death of human host cells, which may accelerate the autoimmune process
Maalouf et al., Mol Carcinog 2002 : Exposure of human skin to UVB radiation leads to the depletion of cutaneous antioxidants, the activation of nuclear factor kappa B (NF-kappaB) , and programmed cell death ( apoptosis )
Delgado et al., Glia 2002 (Encephalitis) : The VIP/PACAP inhibition of both chemokine production and of NFkB binding is mediated through the specific receptor VPAC1 and involves a cAMP dependent intracellular pathway ... The VIP/PACAP inhibition of both chemokine production and of NFkB binding is mediated through the specific receptor VPAC1 and involves a cAMP dependent intracellular pathway
Li et al., Clin Cancer Res 2002 (Prostatic Neoplasms) : Moreover, genistein abrogated the epidermal growth factor induced activation of NF-kappaB , which was mediated via Akt signaling pathway
Ochi et al., Eur J Immunol 2002 : Transcriptional up-regulation of the VCAM-1 gene, induced by proinflammatory cytokines such as IL-1beta and TNF-alpha, requires activation of not only NF-kappaB , but also involves interferon regulatory factor (IRF)-1 ... Transcriptional up-regulation of the VCAM-1 gene, induced by proinflammatory cytokines such as IL-1beta and TNF-alpha, requires activation of not only NF-kappaB , but also involves interferon regulatory factor (IRF)-1 ... Because induction of both VCAM-1 and E-selectin by these cytokines is NF-kappaB dependent , we investigated whether the inhibitory effect of hyperosmotic medium might involve IRF-1 ... Because induction of both VCAM-1 and E-selectin by these cytokines is NF-kappaB dependent , we investigated whether the inhibitory effect of hyperosmotic medium might involve IRF-1
Ciesielski et al., Eur J Immunol 2002 : As chemokines are important for the progress of an inflammatory response by the recruitment of immuno-competent cells, the role NF-kappaB plays in TNFalpha- or lipopolysaccharides (LPS) induced chemokine secretion by human monocyte derived macrophages was examined
Salmond et al., Eur J Immunol 2002 : CD8+ T cell apoptosis induced by Escherichia coli heat-labile enterotoxin B subunit occurs via a novel pathway involving NF-kappaB dependent caspase activation
Su et al., Nat Immunol 2002 (Lymphoma, B-Cell) : Here, we demonstrate that PKC-beta is specifically required for B cell receptor (BCR) mediated NF-kappa B activation ... Here, we demonstrate that PKC-beta is specifically required for B cell receptor (BCR) mediated NF-kappa B activation
Cook et al., Proc Natl Acad Sci U S A 2002 : E1A expression did not block upstream events in TNF-alpha induced activation of NF-kappa B in NIH 3T3 cells, including degradation of I kappa B-alpha, nuclear translocation of NF-kappa B subunits, and their dimeric binding to kappa B sequences in the nucleus
Zimmerman et al., Am J Physiol Regul Integr Comp Physiol 2002 (Carotid Artery Injuries...) : Expression of the NF-kappaB dependent cytokine monocyte chemotactic protein 1 was markedly diminished in injured TNF ( -/- ) animals
Tergaonkar et al., Cancer Cell 2002 : IKK2 mediated effects required its kinase function and were abrogated by coexpression of the dominant negative IkappaBalphaM, implying a role for NFkappaB in blocking chemotherapy induced p53 and cell death
Dil et al., Vet Immunol Immunopathol 2002 : The objectives of the current study was to determine if the iNOS differences between K and GB2 macrophages are possibly due to differential expression of LPS induced TLR4, CD14 and/or nuclear factor kappa B (NF kappa B)
Masamune et al., Pancreas 2002 (Acute Disease...) : IL-1 beta and TNF-alpha increased the nuclear factor-kappa B (NF-kappa B)-specific and activator protein-1-specific DNA binding activity, whereas the NF-IL6 activity was not altered ... IL-1 beta and TNF-alpha increased the nuclear factor-kappa B (NF-kappa B)-specific and activator protein-1-specific DNA binding activity, whereas the NF-IL6 activity was not altered
Liu et al., Zhonghua Yan Ke Za Zhi 2002 : To evaluate the inductive role of interleukin (IL)-1 beta and the inhibitory role of pyrrolidine dithiocarbamate ( PDTC ) on the activation of nuclear factor (NF)-kappaB in the rat retina ... To evaluate the inductive role of interleukin (IL)-1 beta and the inhibitory role of pyrrolidine dithiocarbamate ( PDTC ) on the activation of nuclear factor (NF)-kappaB in the rat retina ... NF-kappa B in the retina can be activated by intraocular IL-1 beta
Gan et al., Zhonghua Nei Ke Za Zhi 2002 (Colitis, Ulcerative) : ( 2 ) Glucocorticoids and SASP strongly inhibited NF-kappaB activation and signficantly decreased the expression of IL-1beta mRNA and IL-8 mRNA
Sun et al., Frontiers in bioscience : a journal and virtual library 2002 (Arthritis, Rheumatoid) : The results support a stimulatory role of NF-kappa B in transcriptional regulation of MMP-1 and MMP-13 ... The results support a stimulatory role of NF-kappa B in transcriptional regulation of MMP-1 and MMP-13
Matsumoto et al., J Immunol 2002 : NIK mutant T cells showed impaired proliferation and IL-2 production in response to anti-CD3 stimulation, and these effects were caused by impaired NF-kappa B activity in both mature and immature T cells ; the impaired NF-kappa B activity in mature T cells was also associated with the failure of maintenance of activated NF-kappa B
Mancuso et al., J Immunol 2002 : Mitogen activated protein kinases and NF-kappa B are involved in TNF-alpha responses to group B streptococci ... Here we investigate the role of mitogen activated protein kinases ( MAPKs ) and NF-kappa B in TNF-alpha production by human monocytes stimulated with GBS or LPS, used as a positive control ... Selective inhibitors of ERK 1/2 ( PD98059 or U0126 ), p38 ( SB203580 ), or NF-kappa B ( caffeic acid phenetyl ester ( CAPE ) ) could all significantly reduce TNF-alpha production, although none of the inhibitors used alone was able to completely prevent TNF-alpha release
Takami et al., J Immunol 2002 : Moreover, various TLR ligands ( e.g., peptidoglycan, poly ( I:C ) dsRNA, LPS, and CpG motif of unmethylated DNA, which act as ligands for TLR2, 3, 4, and 9, respectively ) induced NF-kappa B activation and up-regulated TNF-alpha production in osteoclast precursor cells
Kim et al., J Immunol 2002 (Acne Vulgaris) : Transfection of TLR2 into a nonresponsive cell line was sufficient for NF-kappa B activation in response to P. acnes
Waldhoer et al., J Virol 2002 : The use of specific inhibitors indicated that the p38 mitogen activated protein ( MAP ) kinase but not the extracellular signal regulated kinase 1/2-MAP kinase pathway is involved in M33- and US28 mediated CREB activation but not NF-kappaB activation
Kiemer et al., Biochem Biophys Res Commun 2002 : ANP reduced TNF-alpha induced NF-kappaB activity, which was paralleled by a decreased translocation of p65 to nuclei ... ANP reduced TNF-alpha induced NF-kappaB activity, which was paralleled by a decreased translocation of p65 to nuclei
Xiao et al., Biol Reprod 2002 : TNFalpha induced IkappaB phosphorylation and NFkappaB activation
Catz et al., Biochem J 2002 (Prostatic Neoplasms) : Co-transfection with NF-kappaB expression vectors or stimulation with TNFalpha resulted in significant transactivation of the jfc1 promoter construct, although transactivation of a mutated jfc1 promoter was negligible
Yu et al., J Am Soc Nephrol 2002 : TSA inhibited induction of endogenous NO production and iNOS as well as NF-kappa B element promoter activity in response to interleukin-1 beta (IL-1 beta) or lipopolysaccharide (LPS) + interferon-gamma (IFN-gamma) in both cell types without altering NF-kappa B DNA binding activity ... TSA inhibited induction of endogenous NO production and iNOS as well as NF-kappa B element promoter activity in response to interleukin-1 beta (IL-1 beta) or lipopolysaccharide (LPS) + interferon-gamma (IFN-gamma) in both cell types without altering NF-kappa B DNA binding activity ... TSA inhibited induction of endogenous NO production and iNOS as well as NF-kappa B element promoter activity in response to interleukin-1 beta (IL-1 beta) or lipopolysaccharide (LPS) + interferon-gamma (IFN-gamma) in both cell types without altering NF-kappa B DNA binding activity ... TSA inhibited induction of endogenous NO production and iNOS as well as NF-kappa B element promoter activity in response to interleukin-1 beta (IL-1 beta) or lipopolysaccharide (LPS) + interferon-gamma (IFN-gamma) in both cell types without altering NF-kappa B DNA binding activity
Huang et al., Mol Cell Biol 2002 : Furthermore, deletion and point mutation analyses reveal that both the amino-terminal IKK binding and the carboxy-terminal putative zinc finger domains of NEMO ( IKK gamma ) are critical for UV-induced NF-kappa B activation
Kane et al., Mol Cell Biol 2002 : Akt dependent phosphorylation specifically regulates Cot induction of NF-kappa B-dependent transcription ... Akt appears to function upstream of Cot, as Akt can enhance Cot induction of NF-kappa B-dependent transcription, and dominant negative Cot blocks the activation of this element by Akt ... Akt appears to function upstream of Cot, as Akt can enhance Cot induction of NF-kappa B-dependent transcription, and dominant negative Cot blocks the activation of this element by Akt ... The regulation of NF-kappa B-dependent transcription by Cot requires Akt dependent phosphorylation of serine 400 ( S400 ), near the carboxy terminus of Cot ... This mutated form of Cot also acts as a dominant negative for T-cell antigen receptor/CD28- or Akt/phorbol myristate acetate induced NF-kappa B induction, while having relatively little effect on tumor necrosis factor induction of NF-kappa B
Liedtke et al., Hepatology 2002 (Carcinoma, Hepatocellular...) : TNF dependent activation of NF-kappa B induces the transcription of antiapoptotic genes that renders liver cells resistant against TNF induced apoptosis
Said et al., Br J Pharmacol 2002 : 2 : Although unable to induce PGHS-2 expression by itself, TNF-alpha promoted a similar transcription nuclear factor-kappaB (NF-kappaB) activation to IL-1beta ... HPMEC pre-treatment with MG-132, a proteasome inhibitor, prevented NF-kappaB activation as well as more distal signalling response, indicating that NF-kappaB activation is required but not sufficient for PGHS-2 expression
Mendes et al., Inflamm Res 2002 : SNAP inhibited IL-I induced NF-kappaB activation and NOS II expression ... SNAP inhibited IL-I induced NF-kappaB activation and NOS II expression
Lapteva et al., Biochem Biophys Res Commun 2002 : Our finding indicates the possible activation of NF-kappaB through the up-regulation of expressions of MEFV gene ( encoding PYRIN protein ) and heterogeneous nuclear ribonucleoprotein R in DCs
Roberts et al., Cryobiology 2002 : Inhibition of MAPK with an MEK inhibitor ( PD098059 ) blocked the activation of NF-kappaB but a specific p38 inhibitor ( SB203580 ) had no effect on NF-kappaB ... Inhibition of MAPK with an MEK inhibitor ( PD098059 ) blocked the activation of NF-kappaB but a specific p38 inhibitor ( SB203580 ) had no effect on NF-kappaB
Spiegelman et al., J Biol Chem 2002 (Cell Transformation, Neoplastic) : Activators of mitogen activated protein kinase accelerated IkappaBalpha degradation and increased NF-kappaB transcriptional activity
De Plaen et al., Immunology 2002 : We have previously shown that LPS activates the transcription factor nuclear factor (NF)-kappaB in the intestine, which up-regulates many pro-inflammatory genes ... However, whether LPS and PAF directly activate NF-kappaB in enterocytes remains controversial ... However, whether LPS and PAF directly activate NF-kappaB in enterocytes remains controversial ... In this study, we first investigated the effect of LPS and PAF on NF-kappaB activation in IEC-6 ( a non transformed rat small intestinal crypt cell line ) cells, by electrophoresis mobility shift assay and supershift, and found that LPS, but not PAF , activates NF-kappaB mostly as p50-p65 heterodimers ... In this study, we first investigated the effect of LPS and PAF on NF-kappaB activation in IEC-6 ( a non transformed rat small intestinal crypt cell line ) cells, by electrophoresis mobility shift assay and supershift, and found that LPS , but not PAF, activates NF-kappaB mostly as p50-p65 heterodimers ... Both LPS and TNF induce the expression of the NF-kappaB dependent gene inducible nitric oxide synthase (iNOS) , which occurs subsequent to NF-kappaB activation
Luberto et al., J Biol Chem 2002 : In addition, GW4869 did not significantly impair TNF induced NF-kappaB translocation to nuclei
Wang et al., Nat Immunol 2002 : A requirement for CARMA1 in TCR induced NF-kappa B activation
Gaide et al., Nat Immunol 2002 : Together, our data show that CARMA1 is a critical lipid raft associated regulator of TCR induced NF-kappa B activation and CD28 costimulation dependent Jnk activation
Awad et al., J Med Dent Sci 2002 : Furthermore, pyrrolidine dithiocarbamate ( PDTC ) partially inhibited the IL-1alpha production and completely inhibited NF-kappaB expression by Pam 212 cells
Shibata et al., Breast Cancer Res Treat 2002 (Breast Neoplasms...) : On the other hand, constitutive expression of a transcription factor, NF-kappaB was correlated with progression and metastasis in a number of human breast cancers, suggesting a possible regulation of VEGF expression by NF-kappaB
Kokura et al., Free Radic Biol Med 2002 (Ischemia...) : In particular, oxidant induced activation of the multisubunit nuclear transcription factor , NFkappaB , has been implicated in the transcriptional upregulation of inflammatory genes like endothelial cell adhesion glycoproteins
Jeong et al., Cytokine 2002 : Expression of TNF-alpha and IL-6 was regulated by a transcription factor , nuclear factor (NF)-kappaB ... Expression of TNF-alpha and IL-6 was regulated by a transcription factor, nuclear factor (NF)-kappaB ... Expression of TNF-alpha and IL-6 was regulated by a transcription factor, nuclear factor (NF)-kappaB
Sugino et al., J Clin Endocrinol Metab 2002 : Electrophoretic mobility shift assay revealed that TNF alpha ( 1 ng/ml ) and phorbol 12-myristate 13-acetate ( TPA ; 0.4 micro M ), PKC activator, caused marked increases in nuclear NF-kappa B DNA binding activity
Ward et al., Mol Cell Biochem 2002 (Acute Disease...) : IL-10 and IL-13 attenuate NF-kappaB activation by interfering with breakdown of IkappaBalpha, while SLPI likewise suppresses NF-kappaB activation, but by interfering with breakdown of IkappaBbeta ... IL-10 and IL-13 attenuate NF-kappaB activation by interfering with breakdown of IkappaBalpha, while SLPI likewise suppresses NF-kappaB activation, but by interfering with breakdown of IkappaBbeta ... IL-10 and IL-13 attenuate NF-kappaB activation by interfering with breakdown of IkappaBalpha, while SLPI likewise suppresses NF-kappaB activation, but by interfering with breakdown of IkappaBbeta ... Antibody induced blockade of IL-10, IL-13 or SLPI enhances NF-KB activation in lung and exacerbates the lung inflammatory response and injury ... Antibody induced blockade of IL-10, IL-13 or SLPI enhances NF-KB activation in lung and exacerbates the lung inflammatory response and injury
Rahman et al., Mol Cell Biochem 2002 (Inflammation) : Oxidative stress and TNF-alpha induce histone acetylation and NF-kappaB/AP-1 activation in alveolar epithelial cells : potential mechanism in gene transcription in lung inflammation ... H2O2, and TNF-a , and TSA all increased NF-kappaB and AP-1 DNA binding to their consensus sites assessed by the electrophoretic mobility shift assay ... H2O2, and TNF-a, and TSA all increased NF-kappaB and AP-1 DNA binding to their consensus sites assessed by the electrophoretic mobility shift assay
Ding et al., Mol Cell Biochem 2002 (Cell Transformation, Neoplastic) : This article summarizes recent studies from our laboratory on ( a ) the reduction of Cr ( VI ) by ascorbate, diol- and thiol containing molecules, certain flavoenzymes, cell organelles, intact cells, and whole animals ; ( b ) free radical production in both non-cellular and cellular systems ; and ( c) Cr(VI ) -induced DNA damage, activation of nuclear transcription factor KB ( NF-kappaB ), activator protein-1, p53, hypoxia-inducible factor-1, vascular endothelial growth factor , tyrosine phosphorylation, apoptosis, cell growth arrest, and gene expression profile ... This article summarizes recent studies from our laboratory on ( a ) the reduction of Cr ( VI ) by ascorbate, diol- and thiol containing molecules, certain flavoenzymes, cell organelles, intact cells, and whole animals ; ( b ) free radical production in both non-cellular and cellular systems ; and ( c) Cr(VI ) -induced DNA damage, activation of nuclear transcription factor KB ( NF-kappaB ), activator protein-1 , p53, hypoxia-inducible factor-1, vascular endothelial growth factor, tyrosine phosphorylation, apoptosis, cell growth arrest, and gene expression profile
Hashizume et al., Leuk Res 2002 : Both NAC and PDTC suppressed NF-kappaB binding activity to consensus DNA probe, and this result was correlated with a suppressive effect to CD11a/LFA-1alpha expression
Huang et al., J Virol 2002 : The IL-8 promoter is regulated by NF-kappaB/p65 in response to tumor necrosis factor alpha and requires the cooperation of the coactivators CBP/p300 and steroid receptor coactivator 1 (SRC-1) and the p300/CBP associated factor (P/CAF) for optimal activation
Zoja et al., Kidney Int 2002 (Hemolytic-Uremic Syndrome) : Shiga toxin-2 triggers endothelial leukocyte adhesion and transmigration via NF-kappaB dependent up-regulation of IL-8 and MCP-1 ... Shiga toxin-2 triggers endothelial leukocyte adhesion and transmigration via NF-kappaB dependent up-regulation of IL-8 and MCP-1 ... Stx-2 increased the expression of IL-8 and MCP-1, which was preceded by NF-kappaB activation
Yamasaki et al., Clin Exp Immunol 2002 (Arthritis, Rheumatoid) : Electrophoretic mobility shift assay ( EMSA ) showed a significant reduction in the DNA binding activity of NF-kappaB in troglitazone treated FLS in response to TNF-alpha or IL-1beta ... Electrophoretic mobility shift assay ( EMSA ) showed a significant reduction in the DNA binding activity of NF-kappaB in troglitazone treated FLS in response to TNF-alpha or IL-1beta
Raychaudhuri et al., Clin Immunol 2002 (Hypertension, Pulmonary) : However, mRNA expression was decreased compared to controls for NF-kappaB regulated genes, granulocyte macrophage colony stimulating factor, interleukin-6, macrophage inflammatory protein-1alpha ( MIP-1alpha ), and vascular endothelial growth factor ... However, mRNA expression was decreased compared to controls for NF-kappaB regulated genes, granulocyte macrophage colony stimulating factor, interleukin-6 , macrophage inflammatory protein-1alpha ( MIP-1alpha ), and vascular endothelial growth factor ... However, mRNA expression was decreased compared to controls for NF-kappaB regulated genes, granulocyte macrophage colony stimulating factor, interleukin-6, macrophage inflammatory protein-1alpha ( MIP-1alpha ), and vascular endothelial growth factor ... However, mRNA expression was decreased compared to controls for NF-kappaB regulated genes, granulocyte macrophage colony stimulating factor , interleukin-6, macrophage inflammatory protein-1alpha ( MIP-1alpha ), and vascular endothelial growth factor ... However, mRNA expression was decreased compared to controls for NF-kappaB regulated genes, granulocyte macrophage colony stimulating factor, interleukin-6 , macrophage inflammatory protein-1alpha ( MIP-1alpha ), and vascular endothelial growth factor
Andoh et al., J Immunol 2002 : EMSAs demonstrated that IL-17 did not modulate the TNF-alpha induced NF-kappaB DNA binding activity, but markedly decreased TNF-alpha induced IFN regulatory factor-1 (IRF-1) DNA binding activity
Kumwenda et al., Shock 2002 (Disease Models, Animal...) : Herein, we report that CM-LPS selectively inhibits NF-kappaB in hepatocytes
Wong et al., J Toxicol Environ Health 2002 : Nuclear factor kappaB (NF-kappaB) binding was increased at 2 and 8 h in cells subjected to synchronous and delayed synchronous VT exposure in the presence of LPS
Liu et al., J Biol Chem 2002 : Activation of NF-kappaB as well as C/EBPbeta by RSV-tat , stimulation of RSV-tat induced production of NO by the wild type of p65 and C/EBPbeta, and inhibition of RSV-tat induced production of NO by deltap65, a dominant negative mutant of p65, and deltaC/EBPbeta, a dominant negative mutant of C/EBPbeta, suggest that RSV-tat induces iNOS through the activation of NF-kappaB and C/EBPbeta ... Activation of NF-kappaB as well as C/EBPbeta by RSV-tat, stimulation of RSV-tat induced production of NO by the wild type of p65 and C/EBPbeta, and inhibition of RSV-tat induced production of NO by deltap65, a dominant negative mutant of p65, and deltaC/EBPbeta, a dominant negative mutant of C/EBPbeta, suggest that RSV-tat induces iNOS through the activation of NF-kappaB and C/EBPbeta
Regula et al., J Biol Chem 2002 (MAP Kinase Signaling System) : IKK beta is required for Bcl-2 mediated NF-kappa B activation in ventricular myocytes ... IKK beta is required for Bcl-2 mediated NF-kappa B activation in ventricular myocytes ... The transcription factor nuclear factor kappa B (NF-kappa B) is regulated by cytoplasmic inhibitor I kappa B alpha ... The underlying mechanism by which Bcl-2 activates NF-kappa B is undefined ... In view of growing evidence that the I kappa B kinases ( IKKs ), notably IKK beta, are involved in signal induced phosphorylation of I kappa B alpha, we ascertained whether IKK beta is necessary and sufficient for Bcl-2 mediated NF-kappa B activation ... In view of growing evidence that the I kappa B kinases ( IKKs ), notably IKK beta, are involved in signal induced phosphorylation of I kappa B alpha, we ascertained whether IKK beta is necessary and sufficient for Bcl-2 mediated NF-kappa B activation ... Here we demonstrate that expression of Bcl-2 in ventricular myocytes resulted in an increase in NF-kappa B-dependent DNA binding, NF-kappa B gene transcription and reduced I kappa B alpha levels ... Catalytically inactive mutants of IKK beta, but not IKK alpha, suppressed Bcl-2 mediated I kappa B alpha phosphorylation and NF-kappa B activation ... Transfection of human embryonic 293 cells with a kinase-defective Raf-1 or a kinase-defective mitogen activated protein kinase/extracellular signal regulated kinase kinase-1 ( MEKK-1 ) suppressed Bcl-2 mediated IKK beta activity and NF-kappa B activation ... Further, Bcl-2 mediated NF-kappa B activity was impaired in nullizygous mouse embryonic fibroblasts deficient for IKK beta
Galea et al., Neuroreport 2002 (Inflammation) : We report that 17beta-estradiol ( E ( 2 ) ), but not the alpha-enantiomer, inhibited the basal and interleukin-1beta (IL-1beta) mediated expression of the intercellular adhesion molecule type 1 ( ICAM1 ) and NFkappaB activation, in cultured brain endothelial cells
Soloviev et al., J Orthop Res 2002 : The role of p105 protein in NFkappaB activation in ANA-1 murine macrophages following stimulation with titanium particles
Yoshizaki et al., Histol Histopathol 2002 (Carcinoma...) : LMP-1 down-regulates expression of E-cadherin, induces matrix metalloproteinase-9 and urokinase type-plasminogen activator through activation of NF-kappaB and AP-1, and enhances cell motility via ets-1 activation ... LMP-1 down-regulates expression of E-cadherin, induces matrix metalloproteinase-9 and urokinase type-plasminogen activator through activation of NF-kappaB and AP-1, and enhances cell motility via ets-1 activation ... LMP-1 also induces vascular endothelial growth factor through cyclooxygenase-2 activation and interleukin-8 through NF-kappaB activation ... LMP-1 also induces vascular endothelial growth factor through cyclooxygenase-2 activation and interleukin-8 through NF-kappaB activation
Zhao et al., Biochem J 2002 : However, the signalling mechanisms by which SP-NK-1R interaction induces NF-kappaB activation and IL-8 expression are still not clear ... We also demonstrate that SP rapidly activates RhoA, Rac1 and Cdc42 and that co-expression of the dominant negative mutants of RhoA, Rac1 and Cdc42 in NK-1R cDNA transfected NCM460 cells significantly inhibits SP-induced NF-kappaB dependent gene expression ... We also demonstrate that SP rapidly activates RhoA , Rac1 and Cdc42 and that co-expression of the dominant negative mutants of RhoA, Rac1 and Cdc42 in NK-1R cDNA transfected NCM460 cells significantly inhibits SP-induced NF-kappaB dependent gene expression ... We also demonstrate that SP rapidly activates RhoA, Rac1 and Cdc42 and that co-expression of the dominant negative mutants of RhoA, Rac1 and Cdc42 in NK-1R cDNA transfected NCM460 cells significantly inhibits SP-induced NF-kappaB dependent gene expression
Chang et al., Exp Cell Res 2002 : Even though LTbetaR was shown to recruit TNF-receptor associated factor (TRAF) 2 , 3, and 5, and to induce cell apoptosis or NF-kappaB activation, however, the downstream signaling leading to chemokine expression is not illustrated yet ... Even though LTbetaR was shown to recruit TNF-receptor associated factor (TRAF) 2, 3, and 5, and to induce cell apoptosis or NF-kappaB activation, however, the downstream signaling leading to chemokine expression is not illustrated yet ... This suggests that IL-8 induction by LTbetaR is via TRAFs elicited signaling pathways, including NIK/IKK dependent NF-kappaB activation and ASK/MKK/JNK dependent AP-1 activation ... This suggests that IL-8 induction by LTbetaR is via TRAFs elicited signaling pathways, including NIK/IKK dependent NF-kappaB activation and ASK/MKK/JNK dependent AP-1 activation
Roux et al., Prog Neurobiol 2002 : p75NTR has been shown to activate the NF-kappaB , Akt, and JNK pathways and interacts with several adaptor proteins
Rani et al., J Biol Chem 2002 (Fibrosarcoma) : IFN-beta treatment did not augment the abundance of NF-kappa B , but led to phosphorylation of the NF-kappa B subunit p65 and induced a nuclear activity capable of phosphorylating a p65-GST fusion construct in the carboxy-terminal transactivation domain ( TAD ), residues 354-551
Chendil et al., Cancer Biol Ther 2002 (Prostatic Neoplasms) : Par-4 , a pro-apoptotic gene, inhibits radiation induced NF kappa B activity and Bcl-2 expression leading to induction of radiosensitivity in human prostate cancer cells PC-3 ... Exposure of PC-3 cells to Ad5-I kappa B super-repressor inhibited radiation induced Bcl-2 expression indicating that radiation induced NF kappa B activity is required for the induction of Bcl-2 protein ... PAR-4 was found to inhibit the radiation induced NF kappa B activity and NF kappa B transcriptional activity is essential for bcl-2 upregulation
Mitsiades et al., Oncogene 2002 (Multiple Myeloma) : We have previously shown that Apo2 ligand/TNF Related apoptosis inducing ligand ( Apo2L/TRAIL ) induces apoptosis of MM cells, including cells either sensitive or resistant to Dex and cytotoxic drugs, and overcomes the growth and survival effect of IL-6 ; conversely, NF-kappaB transcriptional activity attenuates their Apo2L/TRAIL-sensitivity ... In contrast, IL-6 does not cause sustained NF-kappaB activation, induces less pronounced Akt activation and FKHRL-1 phosphorylation than IGF-1, and increases the expression of only survivin ... In contrast, IL-6 does not cause sustained NF-kappaB activation, induces less pronounced Akt activation and FKHRL-1 phosphorylation than IGF-1, and increases the expression of only survivin ... In contrast, IL-6 does not cause sustained NF-kappaB activation, induces less pronounced Akt activation and FKHRL-1 phosphorylation than IGF-1, and increases the expression of only survivin ... In contrast, the Akt inhibitor IL-6-Hydroxymethyl-chiro-inositol 2- ( R ) -2-O-methyl-3-O-octadecylcarbonate induced cell death of both Dex- and Doxo-sensitive and -resistant cells ; opposed the protective effect of constitutive Akt activity against Apo2L/TRAIL ; and abrogated the NF-kappaB activation, increase of anti-apoptotic proteins and protection against Apo2L/TRAIL induced by IGF-1
Wu et al., Gene Expr 2002 : In addition, ZAS3 associates with a TNF receptor associated factor to inhibit NF-kappaB- and JNK/ SAPK mediated signaling of TNF-alpha
Cong et al., World J Gastroenterol 2002 (Inflammation) : To elucidate the anti-inflammatory mechanism of an intestinal neuropeptide, sulfated cholecystokinin octapeptide ( sCCK-8 ), the effects of sCCK-8 on lipopolysaccharide (LPS) induced tumor necrosis factor alpha (TNF-alpha) mRNA expression and NF-kappaB activity in pulmonary interstitial macrophages ( PIMs ) were studied ... sCCK-8, at concentrations from 10 ( -8 ) mol.L ( -1 ) to 10 ( -6 ) mol.L ( -1 ) obviously inhibited LPS induced TNF-alpha mRNA expression and NF-kappaB binding activity in a dose dependent manner, P < 0.05, P < 0.01 ... sCCK-8 inhibits LPS induced TNF-alpha mRNA expression by regulating NF-kappaB activity in rat PIMs, which is mediated through CCK receptors and inhibiting IkappaB-alpha degradation ... sCCK-8 inhibits LPS induced TNF-alpha mRNA expression by regulating NF-kappaB activity in rat PIMs, which is mediated through CCK receptors and inhibiting IkappaB-alpha degradation
Yu et al., Ann Clin Lab Sci 2002 : LPS stimulation of rat PBMC increased TNFalpha production and NFkappaB activation, with corresponding loss of IkappaBalpha ... LPS stimulation of rat PBMC increased TNFalpha production and NFkappaB activation, with corresponding loss of IkappaBalpha ... Ketamine significantly reduced the LPS induced NFkappaB activation and inhibited TNFalpha production in a dose dependent manner ... Ketamine significantly reduced the LPS induced NFkappaB activation and inhibited TNFalpha production in a dose dependent manner
Kim et al., Nitric Oxide 2002 : Mercury inhibited LPS induced nuclear translocation of nuclear factor kappaB (NF-kappaB) but had no effect alone
Cho et al., Life Sci 2002 : In this study, the mechanism and structural requirements of resveratrol and related hydroxystilbenes for the inhibition of LPS induced nitric oxide production were studied in macrophage cells ( RAW 264.7 and J774 ) by comparing its effect on LPS induced NF-kappaB translocation and nitric oxide production, and by considering the possibility of involvement of an estrogen receptor
Langen et al., Am J Physiol Cell Physiol 2002 : NAC decreased TNF-alpha induced activation of NF-kappaB only marginally, indicating that the redox-sensitive component of the inhibition of myogenic differentiation by TNF-alpha occurred independently, or downstream of NF-kappaB
Lu et al., Am J Physiol Cell Physiol 2002 : Because TNF-alpha gene expression is under the control of nuclear factor-kappaB (NF-kappaB), we investigated whether FasL can induce NF-kappaB activation and whether such activation plays a role in FasL mediated cell death in macrophages ... Gene transfection studies using NF-kappaB dependent reporter plasmid showed that FasL did activate NF-kappaB promoter activity ... Inhibition of NF-kappaB by a specific NF-kappaB inhibitor, caffeic acid phenylethyl ester, or by dominant expression of the NF-kappaB inhibitory subunit IkappaB caused an increase in FasL induced apoptosis and a reduction in TNF-alpha expression
Chung et al., J Biol Chem 2002 : We show that BRAM1 interferes with LMP1 mediated NF-kappaB activation but not the JNK signaling pathway ... Because the CTAR2 region interacts with the tumor necrosis factor ( TNF-alpha receptor associated death domain protein, it is interesting to find that BRAM1 also interferes with NF-kappaB activation mediated by TNF-alpha ... BRAM1 interferes LMP1 mediated and TNF-alpha induced NF-kappaB activation by targeting IkappaBalpha molecules
Ruby et al., Mol Pharmacol 2002 : 2,3,7,8-Tetrachlorodibenzo-p-dioxin suppresses tumor necrosis factor-alpha and anti-CD40 induced activation of NF-kappaB/Rel in dendritic cells : p50 homodimer activation is not affected ... Dendritic cells (DCs) , considered to be the `` pacemakers '' of the immune system, were recently recognized as targets of TCDD and are also dependent on NF-kappaB/Rel for activation and survival
Cho et al., Infect Immun 2002 (Scrub Typhus...) : Although NF-kappaB activation was observed in HUVEC infected with O. tsutsugamushi, inhibition of NF-kappaB activation did not affect the MCP-1 expression
Anto et al., Carcinogenesis 2002 : Activation of NF-kappaB by CSC correlated with time dependent degradation of IkappaB(alpha) , an inhibitor of NF-kappaB
Ainbinder et al., Mol Cell Biol 2002 : Here we report that even though NF-kappaB interacts directly with TAF(II)s, induction of NF-kappaB by tumor necrosis factor alpha (TNF-alpha) does not enhance TFIID recruitment and preinitiation complex formation on some NF-kappaB-responsive promoters ... Here we report that even though NF-kappaB interacts directly with TAF(II)s, induction of NF-kappaB by tumor necrosis factor alpha (TNF-alpha) does not enhance TFIID recruitment and preinitiation complex formation on some NF-kappaB-responsive promoters
Makris et al., Mol Cell Biol 2002 : Correspondingly, IKK and NF-kappaB activation by TNF-alpha and, to a lesser extent, IL-1 are reduced
Ohkita et al., Clin Sci (Lond) 2002 : In addition, FK409 diminished basal and TNF-alpha stimulated NF-kappa B activation in ECs
Wesemann et al., J Immunol 2002 : IFN-gamma leads to STAT-1alpha activation directly and up-regulation of NF-kappaB activity due to the secretion and subsequent autocrine signaling of TNF-alpha ... Additionally, IFN-gamma induced TNF-alpha secretion, as well as STAT-1alpha and NF-kappaB activation, are inhibited in the presence of SOCS-1 ... Additionally, IFN-gamma induced TNF-alpha secretion, as well as STAT-1alpha and NF-kappaB activation , are inhibited in the presence of SOCS-1 ... We conclude that SOCS-1 inhibits cytokine induced CD40 expression by blocking IFN-gamma mediated STAT-1alpha activation, which also then results in suppression of IFN-gamma induced TNF-alpha secretion and subsequent NF-kappaB activation
Lee et al., J Immunol 2002 : In WT cells, Vit D and IL-1 stimulated receptor activator of NF-kappaB ligand mRNA expression by 3- and 4-fold, respectively, and their combination produced a 7-fold increase
Kabouridis et al., J Immunol 2002 (MAP Kinase Signaling System) : TAT-srIkappaBalpha, when exogenously added to HeLa cells, inhibited in a dose dependent manner TNF-alpha- or IL-1beta induced NF-kappaB activation and binding of NF-kappaB to its consensus DNA sequence
Hull et al., J Immunol 2002 : In contrast, even though LPS can also activate NF-kappaB , this signal is dispensable for LPS-inducible cytoprotective activity ... In this study, we demonstrate that the C-terminal fragment of TRAF6 ( TRAF6-C ) inhibits LPS induced NF-kappaB nuclear translocation and c-Jun NH ( 2 ) -terminal kinase ( JNK ) activation in endothelial cells
Smith et al., J Immunol 2002 (Inflammation) : We also demonstrated that the induction of Cox-2 was regulated by 3O-C ( 12 ) -HSL activation of the transcription factor NF-kappaB ... We also demonstrated that the induction of Cox-2 was regulated by 3O-C ( 12 ) -HSL activation of the transcription factor NF-kappaB
Gutierrez et al., J Biol Chem 2002 (Crohn Disease...) : Nod2 , a member of the Apaf1/Nod protein family, confers responsiveness to bacterial products and activates NF-kappaB , a transcription factor that plays a central role in innate immunity
Yuksel et al., Thromb Haemost 2002 (MAP Kinase Signaling System...) : APC inhibited both the binding of nuclear factor-kappa B (NF-kappa B) to target sites and the degradation of I kappa B alpha ... These observations strongly suggest that APC inhibited LPS induced TNF-alpha production by inhibiting the activation of both NF-kappa B and AP-1 and that the inhibitory activity of APC might depend on its serine protease activity ... These observations strongly suggest that APC inhibited LPS induced TNF-alpha production by inhibiting the activation of both NF-kappa B and AP-1 and that the inhibitory activity of APC might depend on its serine protease activity
Syrovets et al., Thromb Haemost 2002 (Inflammation) : Ciglitazone activates PPAR gamma as shown by a novel surface plasmon resonance analysis and inhibits the plasmin induced activation of NF-kappa B and AP-1
Muroi et al., J Biol Chem 2002 : Regions of mouse CD14 required for Toll-like receptor 2 (TLR2)- and TLR4 mediated activation of NF-kappaB were studied in transiently transfected 293 cells
Pinkoski et al., J Biol Chem 2002 : TNFalpha induced activation of the Fas-ligand promoter in IEC requires NF-kappaB as this was blocked by an I-kappaBalphaM super-repressor and by mutation of an NF-kappaB site in the Fas-ligand promoter ... TNFalpha induced activation of the Fas-ligand promoter in IEC requires NF-kappaB as this was blocked by an I-kappaBalphaM super-repressor and by mutation of an NF-kappaB site in the Fas-ligand promoter
Schauvliege et al., J Biol Chem 2002 : Transient transfections with caspase-11 promoter-luciferase reporter constructs and deletion/mutation analysis revealed an essential role for NF-kappaB binding in the up-regulation of caspase-11 in response to LPS
Kaku et al., Int Immunol 2002 : Activation of NF-kappaB-like proteins by CD38 ligation is not observed in splenic B cells from Bruton's tyrosine kinase (Btk)-deficient ( Btk ( -/- ) ) mice, with inhibitors of protein kinase C ( PKC ) and phosphatidylinositol (PI)-3 kinase also suppressing NF-kappaB activation in CD38 activated B cells ... We infer from these results that activation of Btk , PI-3 kinase and PKC play, at least in part, important roles in the induction of NF-kappaB in CD38 stimulated murine B cells
Park et al., Science 2002 (MAP Kinase Signaling System...) : Because macrophages that are deficient in transcription factor nuclear factor kappaB (NF-kappaB) are also sensitive to activation induced death and p38 is required for expression of certain NF-kappaB target genes, p38 is probably essential for synergistic induction of those NF-kappaB target genes that prevent apoptosis of activated macrophages ... Because macrophages that are deficient in transcription factor nuclear factor kappaB (NF-kappaB) are also sensitive to activation induced death and p38 is required for expression of certain NF-kappaB target genes, p38 is probably essential for synergistic induction of those NF-kappaB target genes that prevent apoptosis of activated macrophages
Lasek et al., Cancer Chemother Pharmacol 2002 (Melanoma...) : The role of NF-kappaB in enhancement of TNF-alpha production was confirmed in experiments in which MG132, an inhibitor of NF-kappaB activation, reversed the effect of AdAMP
Tselepis et al., Oncogene 2002 (Adenocarcinoma...) : This beta-catenin mediated transcription is independent of NF-kappaB activation
Shetty et al., Apoptosis 2002 : Tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) up-regulates death receptor 5 (DR5) mediated by NFkappaB activation in epithelial derived cell lines ... By blocking TRAIL induced NFkappaB activation, the sensitivity of cells to undergo TRAIL induced apoptosis was significantly decreased
Scheller et al., Eur J Immunol 2002 (Inflammation...) : Further analysis with A3.01 T cells revealed that the proinflammatory signaling activity of CD95 was mediated by MEK/ERK, p38 and NF-kappaB signaling pathways
Kim et al., Biochem Biophys Res Commun 2002 : In addition, merlin blocked the tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB-DNA binding mediated via the inhibition of degradation of IkappaBalpha and blocked the activation of NF-kappaB dependent transcription
Díez-Fernández et al., Free Radic Biol Med 2002 : Heat treatment also prevented TGF-beta1 induced activation of nuclear factor kappa B (NF-kappaB) by preventing the degradation of the inhibitory protein kappa Balpha ( IkappaBalpha )
Colell et al., FEBS Lett 2002 (Colonic Neoplasms) : Exogenous neutral ( N ) and acidic ( A ) SMase activated NF-kappa B with different kinetics, accounting for the diverse pattern of DNA binding of NF-kappa B complexes activated by tumor necrosis factor-alpha (TNF)
Wu et al., FEBS Lett 2002 : In HEK293 cells, proteasome inhibitors could concentration-dependently increase IL-8 promoter and activator protein-1 (AP-1) activities, but inhibited nuclear factor (NF)-kappa B activation induced by cytokines ... In HEK293 cells, proteasome inhibitors could concentration-dependently increase IL-8 promoter and activator protein-1 (AP-1) activities, but inhibited nuclear factor (NF)-kappa B activation induced by cytokines
Bozinovski et al., J Biol Chem 2002 (MAP Kinase Signaling System) : Granulocyte/macrophage-colony stimulating factor ( GM-CSF ) regulates lung innate immunity to lipopolysaccharide through Akt/Erk activation of NFkappa B and AP-1 in vivo ... Granulocyte/macrophage-colony stimulating factor ( GM-CSF ) regulates lung innate immunity to lipopolysaccharide through Akt/Erk activation of NFkappa B and AP-1 in vivo
Hinz et al., J Exp Med 2002 (Hodgkin Disease) : Intriguingly, NF-kappaB positively regulates STAT5a expression and signaling pathways in HRS cells, and promotes its persistent activation
Singh et al., Arthritis Rheum 2002 (Osteoarthritis, Knee) : The inhibition of NO production correlated with the suppression of induction and expression of NF-kappaB dependent gene iNOS ... EGCG inhibited the activation and translocation of NF-kappaB to the nucleus by suppressing the degradation of its inhibitory protein IkappaBalpha in the cytoplasm
Harlin et al., Eur J Immunol 2002 : CTLA-4 engagement regulates NF-kappaB activation in vivo ... In vitro, ligation of CTLA-4 reduces TCR mediated activation of NF-kappaB , a transcription factor implicated in promoting T cell survival and cytokine production ... However, whether NF-kappaB inhibition downstream of CTLA-4 is necessary for down-regulation of T cell responses is not known
Hipp et al., Eur J Immunol 2002 : Inhibition of the proteasome by MG-132 or lactacystin completely blocked TNF-alpha induced IkappaBalpha degradation as well as NF-kappaB activity in human arterial endothelial cells
Stratton et al., Prostate 2002 (Carcinoma...) : Furthermore, testosterone completely abrogated NF kappa B transactivation activity and induction of IL-6 protein expression and mRNA
Deregowski et al., Biochem Pharmacol 2002 (Breast Neoplasms...) : Our results showed an NF-kappaB dependent induction of ephrin-A1 and caveolin-1 mRNAs after stimulation with TNF-alpha and IL-1beta, confirming that NF-kappaB controls target genes implied in tumor angiogenesis and cell transformation ... Our results showed an NF-kappaB dependent induction of ephrin-A1 and caveolin-1 mRNAs after stimulation with TNF-alpha and IL-1beta, confirming that NF-kappaB controls target genes implied in tumor angiogenesis and cell transformation
Rahman et al., Biochem Pharmacol 2002 (Pneumonia) : Pharmacological inhibition of HDAC leads to the increased HAT activity, AP-1 and NF-kappaB activation, and IL-8 release by H2O2 or TNF-alpha treatments
Hippenstiel et al., Biochem Pharmacol 2002 : In order to analyze the role of Rho proteins in TNF-alpha induced NF-kappaB-activation in human umbilical cord vein endothelial cells ( HUVEC ) we used Clostridium difficile toxin B-10463 ( TcdB-10463 ) which inactivates RhoA/Rac1/Cdc42 by glucosylation and Clostridium botulinum C3-toxin which inhibits RhoA/B/C by ADP-ribosylation ... In order to analyze the role of Rho proteins in TNF-alpha induced NF-kappaB-activation in human umbilical cord vein endothelial cells ( HUVEC ) we used Clostridium difficile toxin B-10463 ( TcdB-10463 ) which inactivates RhoA/Rac1/Cdc42 by glucosylation and Clostridium botulinum C3-toxin which inhibits RhoA/B/C by ADP-ribosylation ... Blocking of Rho reduced NF-kappaB DNA binding as shown by electrophoretic mobility shift assays ... Therefore, TNF-alpha related Ikappa-B-degradation is Rho independent in HUVEC, whereas a Rho protein dependent signal is necessary to induce nuclear transport of NF-kappaB in these cells pointing to a novel and unique role of Rho in NF-kappaB-translocation
Rahman et al., Circ Res 2002 : Cotransfection of RGS3T, a regulator of G-protein signaling that inhibits Galpha ( q ), or alpha-transducin ( Galpha ( t ) ), a scavenger of the Gbetagamma, markedly decreased NF-kappaB activity induced by PAR-1 activation ... Expression of the kinase-defective protein kinase C (PKC)-delta mutant inhibited NF-kappaB activation induced by the constitutively active Galpha ( q ) mutant, but had no effect on NF-kappaB activity induced by Gbeta ( 1 ) gamma ( 2 ) ... Cotransfection of the catalytically inactive Akt mutant inhibited the NF-kappaB activation induced by the constitutively active PI 3-kinase mutant as well as that by the activated forms of Galpha ( q ) and PKC-delta ... These results support a model in which ligation of PAR-1 induces NF-kappaB activation and ICAM-1 transcription by the engagement of parallel Galphaq/PKC-delta and Gbetagamma/PI3-kinase pathways that converge at Akt
Inoue et al., Lab Invest 2002 (Adenocarcinoma...) : Therefore, we investigated TRAIL sensitivity, TRAIL induced nuclear factor-kappaB (NF-kappaB) activation, and expression of TRAIL in human colonic adenocarcinoma cell lines ( HT-29, LS180, SK-CO-1 ) ... Activation of NF-kappaB was variably influenced by TRAIL administration, with no consistent tendency among the cell lines, indicating that TRAIL induced NF-kappaB activation might be cell-type dependent
Oda et al., J Immunol 2002 : Although NF-kappaB is involved in regulating the IL-1alpha induced expression of MCP-1 and IL-8, the degradation of IkappaB-alpha stimulated by IL-1alpha was not inhibited by alprazolam ... Although NF-kappaB is involved in regulating the IL-1alpha induced expression of MCP-1 and IL-8 , the degradation of IkappaB-alpha stimulated by IL-1alpha was not inhibited by alprazolam ... Alprazolam prevented NF-kappaB from binding to the MCP-1 promoter region ( the A2 and A1 oligonucleotide probes ), but binding of NF-kappaB to IL-8/NF-kappaB was not inhibited ... These results show that the inhibition of IL-1alpha mediated MCP-1 production by alprazolam is mainly due to inhibition of c-Rel/p65 and c-Rel/p50 binding to the MCP-1 promoter region, since alprazolam did not affect the IL-1alpha mediated activation of NF-kappaB ( p50/p65 ) or AP-1 ( c-Jun/c-Fos ) binding to the IL-8 promoter region
Thomas et al., Surgery 2002 (Pancreatic Neoplasms) : FLICE-like inhibitory protein (FLIP) , an inhibitor of caspase-8, ( also known as FLICE ) is regulated by the transcription factor nuclear factor-kappaB (NF-kappaB) and can contribute to TRAIL resistance
Badgwell et al., Surgery 2002 (Escherichia coli Infections...) : The 2 groups of mice were analyzed for serum levels of interferon-gamma, tumor necrosis factor-alpha , and interleukin-1beta as well as activation of NFkappaB and STAT1, 2 proinflammatory transcription factors ... The 2 groups of mice were analyzed for serum levels of interferon-gamma , tumor necrosis factor-alpha, and interleukin-1beta as well as activation of NFkappaB and STAT1, 2 proinflammatory transcription factors ... The 2 groups of mice were analyzed for serum levels of interferon-gamma, tumor necrosis factor-alpha, and interleukin-1beta as well as activation of NFkappaB and STAT1, 2 proinflammatory transcription factors
Hassoun et al., Surgery 2002 (Aortic Aneurysm, Abdominal...) : We examined the effects of regional intraischemic hypothermia on mesenteric I/R induced mucosal injury, NF-kappaB activation , and expression of iNOS and HO-1 ... We examined the effects of regional intraischemic hypothermia on mesenteric I/R induced mucosal injury, NF-kappaB activation , and expression of iNOS and HO-1 ... Mesenteric I/R significantly increased intestinal permeability to ( 14 ) C inulin, histologic injury, activation of NF-kappaB , and iNOS and HO-1 expression when compared with sham control rats ... Mesenteric I/R significantly increased intestinal permeability to ( 14 ) C inulin, histologic injury, activation of NF-kappaB , and iNOS and HO-1 expression when compared with sham control rats
Yang et al., Cell Signal 2002 (Asthma...) : IL-1beta induced activation of NF-kappaB correlated with the degradation of IkappaB-alpha in TSMCs. IL-1beta induced NF-kappaB activation, COX-2 expression, and PGE ( 2 ) synthesis were inhibited by the dominant negative mutants of NIK and IKK-alpha , but not by IKK-beta ... IL-1beta induced activation of NF-kappaB correlated with the degradation of IkappaB-alpha in TSMCs. IL-1beta induced NF-kappaB activation, COX-2 expression, and PGE ( 2 ) synthesis were inhibited by the dominant negative mutants of NIK and IKK-alpha, but not by IKK-beta ... IL-1beta induced activation of NF-kappaB correlated with the degradation of IkappaB-alpha in TSMCs. IL-1beta induced NF-kappaB activation, COX-2 expression, and PGE ( 2 ) synthesis were inhibited by the dominant negative mutants of NIK and IKK-alpha, but not by IKK-beta ... IL-1beta induced COX-2 expression and PGE ( 2 ) synthesis were completely inhibited by PD98059 ( an inhibitor of MEK1/2 ) and SB203580 ( an inhibitor of p38 inhibitor ), but these two inhibitors had no effect on IL-1beta induced NF-kappaB activation, indicating that activation of p42/44 and p38 MAPK and NF-kappaB signalling pathways were independently required for these responses
Oh et al., Planta Med 2002 : Catalposide ( 1 ) also inhibited the activation of LPS induced NF-kappaB as analyzed by electrophoretic mobility shift assay ( EMSA )
La Ferla et al., FASEB J 2002 : Thus, both GATA-2 and NF-kappaB seem to be involved in the suppression of Epo gene expression by IL-1beta and TNF-alpha in vitro and may be responsible for impaired Epo synthesis in inflammatory diseases in vivo
Li et al., Am J Physiol Lung Cell Mol Physiol 2002 (MAP Kinase Signaling System) : Recent studies indicate that maximal IL-8 protein expression requires activation of NF-kappaB as well as activation of the MAP kinases ERK, JNK, and p38 ... Recent studies indicate that maximal IL-8 protein expression requires activation of NF-kappaB as well as activation of the MAP kinases ERK , JNK, and p38 ... Recent studies indicate that maximal IL-8 protein expression requires activation of NF-kappaB as well as activation of the MAP kinases ERK, JNK, and p38 ... Inhibition of JNK signaling also substantially reduced TNF-alpha induced NF-kappaB transactivation, whereas inhibition of ERK and p38 had no effect
Billack et al., Am J Physiol Cell Physiol 2002 : Mobility shift assays revealed that HSP60 induced NF-kappaB and CRE binding activity, while CCAAT/enhancer binding protein (C/EBP), which binds to NF-IL-6, was constitutively active in the cells ... Mobility shift assays revealed that HSP60 induced NF-kappaB and CRE binding activity, while CCAAT/enhancer binding protein (C/EBP) , which binds to NF-IL-6, was constitutively active in the cells
Kobayashi et al., Environmental medicine : annual report of the Research Institute of Environmental Medicine, Nagoya University 1999 : In the present study, we investigated the effects of vector averaged gravity ( clinostat rotation ) on tumor necrosis factor (TNF)-alpha induced activation of NF-kappa B in human osteoblastic HOS-TE85 cells ... These results indicate that exposure of HOS-TE85 cells to vector averaged gravity impairs NF-kappa B activation by TNF-alpha
Kwon et al., Biol Pharm Bull 2002 (Inflammation) : Expression of cyclooxygenase-2 and pro-inflammatory cytokines induced by 2,2',4,4',5,5'-hexachlorobiphenyl ( PCB 153 ) in human mast cells requires NF-kappa B activation ... However, pre treating them with a NF-kappaB pathway inhibitor, pyrrolidine dithiocarbamate, suppressed PCB induced NF-kappaB activation
Sung et al., Kidney Int 2002 (Reperfusion Injury) : Enhanced MCP-1 expression during ischemia/reperfusion injury is mediated by oxidative stress and NF-kappaB ... Pretreatment of rats with NF-kappaB inhibitors not only prevented NF-kappaB activation induced by ischemia/reperfusion, but also inhibited MCP-1 mRNA expression ... The present study clearly demonstrates that enhanced MCP-1 expression in rat kidney during ischemia/reperfusion injury is mediated by NF-kappaB activation and oxidative stress
Pajonk et al., Cancer Res 2002 (Prostatic Neoplasms) : The effect of saquinavir on LPS/IFN-gamma induced activation of NF-kappaB was assessed by gel-shift assays and by Western analysis of corresponding IkappaBalpha-levels ... The effect of saquinavir on LPS/IFN-gamma induced activation of NF-kappaB was assessed by gel-shift assays and by Western analysis of corresponding IkappaBalpha-levels ... Saquinavir treatment prevented LPS/IFN-gamma induced activation of NF-kappaB in RAW cells and stabilized expression of IkappaBalpha ... Saquinavir treatment prevented LPS/IFN-gamma induced activation of NF-kappaB in RAW cells and stabilized expression of IkappaBalpha
József et al., Proc Natl Acad Sci U S A 2002 : Lipoxin A4 and aspirin triggered 15-epi-lipoxin A4 inhibit peroxynitrite formation, NF-kappa B and AP-1 activation, and IL-8 gene expression in human leukocytes
Ikeda et al., Cardiovasc Res 2002 (Cardiomyopathy, Dilated...) : Addition of IL-1beta activated NF-kappaB in cardiac myocytes, while CRP did not affect IL-1beta induced NF-kappaB activation ... Addition of IL-1beta activated NF-kappaB in cardiac myocytes, while CRP did not affect IL-1beta induced NF-kappaB activation
Spiecker et al., Mol Pharmacol 2002 : We studied the effects of tranilast on nuclear factor-kappaB (NF-kappaB) dependent endothelial cell adhesion molecule expression and transcriptional regulation
Wakabayashi et al., Naunyn Schmiedebergs Arch Pharmacol 2002 : On the other hand, LPS induced translocation of nuclear factor-kappaB (NF-kappaB) was not affected significantly by 100-600 mM ethanol
Hershko et al., Clin Sci (Lond) 2002 : We tested the hypothesis that sodium arsenite inhibits IL-6 production in stimulated enterocytes and that this effect of arsenite is caused by down-regulation of NF-kappaB activity ... IL-1beta increased NF-kappaB DNA binding activity, IL-6 mRNA levels and IL-6 production
Wu et al., Mol Cell Endocrinol 2002 : Advanced glycosylation end products induce NF-kappaB dependent iNOS expression in RAW 264.7 cells
Qin et al., J Immunol 2002 (Leukemia, Myeloid, Acute) : Fas resistance of leukemic eosinophils is due to activation of NF-kappa B by Fas ligation ... TNF family receptors can lead to the activation of NF-kappaB and this can be a prosurvival signal in some cells ... Although activation of NF-kappaB by ligation of Fas ( CD95/Apo-1 ), a member of the TNFR family, has been observed in a few studies, Fas mediated NF-kappaB activation has not previously been shown to protect cells from apoptosis ... We examined the Fas induced NF-kappaB activation and its antiapoptotic effects in a leukemic eosinophil cell line, AML14.3D10, an AML14 subline resistant to Fas mediated apoptosis ... Stable transfection and overexpression of IkappaBalpha in 3D10 cells inhibited CH11 induced NF-kappaB activation and completely abrogated Fas resistance
Chen et al., Life Sci 2002 : We also evaluated their effect on LPS stimulated NF-kappaB transcriptional activity in a THP-1 cell line, and on human monocyte chemotactic response to chemoattractants ... QKL ( 1 % ) did not inhibit monocyte chemotaxis induced by super-or sub-optimal concentrations of fMLP ( 10 ( -5 ), 10 ( -6 ) and 10 ( -10 ) M ), but only inhibited chemotaxis induced by optimal concentrations of fMLP at 10 ( -7 ), 10 ( -8 ) and 10 ( -9 ) M. QKL ( 0.1 % or 1 % ) and SHHL ( 6 or 60 microg/ml ) markedly inhibited LPS induced NF-kappaB activity in THP-1 cells
Lee et al., J Biochem Mol Biol 2002 : TAK1 dependent activation of AP-1 and c-Jun N-terminal kinase by receptor activator of NF-kappaB ... TAK1 dependent activation of AP-1 and c-Jun N-terminal kinase by receptor activator of NF-kappaB ... The inhibitory effect of the TAK1 mutant on RANK- and TRAF induced NF-kappaB activation was also observed, but less efficiently ... The inhibitory effect of the TAK1 mutant on RANK- and TRAF induced NF-kappaB activation was also observed, but less efficiently
Drumm et al., Cell Physiol Biochem 2002 : The aim of this study was to investigate albumin induced NF-kappaB expression as well as NF-kappaB activity upon long long term exposure to albumin in human proximal tubular cells as only acute albumin induced NF-kappaB activity has been reported so far ... An albumin induced increase in TNF-alpha specific mRNA expression as biological, inflammatory parameter associated with the albumin induced NF-kappaB activity was detectable
Li et al., Respir Res 2002 : LPS triggered NF-kappaB activation, a mechanism responsible for the iNOS expression
Nakao et al., Mol Cell Biochem 2002 : Tumor necrosis factor alpha (TNF-alpha) induced prostaglandin E2 release is mediated by the activation of cyclooxygenase-2 (COX-2) transcription via NFkappaB in human gingival fibroblasts
An et al., Int Immunopharmacol 2002 : Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don ( Bignoniaceae ), was found to inhibit the productions of tumor necrosis factor-alpha (TNF-alpha) , interleukin-1beta (IL-1beta), and interleukin-6 (IL-6), and the activation of nuclear factor kappaB (NF-kappaB) in RAW 264.7 macrophages activated with lipopolysaccharide (LPS) ... Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don ( Bignoniaceae ), was found to inhibit the productions of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta) , and interleukin-6 (IL-6), and the activation of nuclear factor kappaB (NF-kappaB) in RAW 264.7 macrophages activated with lipopolysaccharide (LPS) ... Catalposide, the major iridoid glycoside isolated from the stem bark of Catalpa ovata G. Don ( Bignoniaceae ), was found to inhibit the productions of tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and interleukin-6 (IL-6) , and the activation of nuclear factor kappaB (NF-kappaB) in RAW 264.7 macrophages activated with lipopolysaccharide (LPS)
Claudio et al., Nat Immunol 2002 : We show here that B cell activating factor ( BAFF ) activates this second pathway and that this requires the BAFF receptor (BAFF-R), the NF-kappa B-inducing kinase (NIK) and protein synthesis, but not NEMO
Glauner et al., Eur J Biochem 2002 : In cells deficient for RIP ( receptor interactive protein ), an essential signaling intermediate of TNF dependent NF-kappaB activation, TNF-, but not PMA induced up-regulation of TRAF4 was blocked
Chanani et al., Circulation 2002 (Inflammation) : Both LPS and TNF-alpha induced significant NFkappaB activation, cyclooxygenase-2 (COX-2) expression, and inducible NO synthase (iNOS) and cytokine production ; with the exception of COX-2 expression, all were significantly reduced by amrinone, beginning at concentrations of 10 to 50 micro mol/L ... Both LPS and TNF-alpha induced significant NFkappaB activation, cyclooxygenase-2 (COX-2) expression, and inducible NO synthase (iNOS) and cytokine production ; with the exception of COX-2 expression, all were significantly reduced by amrinone, beginning at concentrations of 10 to 50 micro mol/L
Lin et al., J Pharm Pharmacol 2002 : Treatment of cells with byakangelicol ( 50 microM ) or pyrrolidine dithiocarbamate ( PDTC ; 50 microM ) partially inhibited IL-1beta induced degradation of IkappaB-alpha in the cytosol, translocation of p65 NF-kappaB from the cytosol to the nucleus and the NF-kappaB-specific DNA-protein complex formation
Andrassy et al., Clin Nephrol 2002 (Anemia...) : Erythropoietin mediated decrease of the redox-sensitive transcription factor NF-kappaB is inversely correlated with the hemoglobin level ... The aim of this study was to determine the effect of rh-EPO on the redox-sensitive transcription factor ( NF-kappaB ) in vivo and in vitro ... In vitro studies showed that rh-EPO did not directly affect NF-KB binding activity in THP-1 cells
Halvorsen et al., Brain Res 2002 (MAP Kinase Signaling System...) : Survival promoting MEK-ERK and PI3K pathways contribute to viability during MPP ( + ) exposure, both are activated by NGF , and loss of PI3K mediated signaling and NF-kappa B-mediated transcription may commit cells irreversibly to apoptosis in this model
Chiao et al., Cancer 2002 (Carcinoma, Hepatocellular...) : The current study data demonstrate that 1 ) RelA/NF-kappaB activity is activated constitutively in Hep3B cells, as determined by electrophoretic mobility shift assays ; 2 ) RelA/NF-kappaB reporter gene activity is inhibited specifically by dominant negative mutants of IkappaB(alpha), IKK1 , IKK2, MEKK1, and MEKK3 and it is activated by overexpression of wild-type MEKK3, suggesting that upstream kinase cascades induce phosphorylation of IkappaB(alpha) and activate RelA/NF-kappaB in Hep3B cells ; 3 ) overexpression of the HBV x gene fails to activate NF-kappaB in HepG2 and Chang cell lines ; 4 ) The NF-kappaB-inducible gene, bcl-xl, is overexpressed in Hep3B cells and is inhibited by the proteosome inhibitor PS341, which prevents IkappaBalpha degradation and RelA/NF-kappaB activation ; and 5 ) inhibition of constitutive RelA/NF-kappaB activity by PS341 sensitizes Hep3B cells to doxorubicin induced apoptosis ... The current study data demonstrate that 1 ) RelA/NF-kappaB activity is activated constitutively in Hep3B cells, as determined by electrophoretic mobility shift assays ; 2 ) RelA/NF-kappaB reporter gene activity is inhibited specifically by dominant negative mutants of IkappaB(alpha), IKK1, IKK2 , MEKK1, and MEKK3 and it is activated by overexpression of wild-type MEKK3, suggesting that upstream kinase cascades induce phosphorylation of IkappaB(alpha) and activate RelA/NF-kappaB in Hep3B cells ; 3 ) overexpression of the HBV x gene fails to activate NF-kappaB in HepG2 and Chang cell lines ; 4 ) The NF-kappaB-inducible gene, bcl-xl, is overexpressed in Hep3B cells and is inhibited by the proteosome inhibitor PS341, which prevents IkappaBalpha degradation and RelA/NF-kappaB activation ; and 5 ) inhibition of constitutive RelA/NF-kappaB activity by PS341 sensitizes Hep3B cells to doxorubicin induced apoptosis ... The current study data demonstrate that 1 ) RelA/NF-kappaB activity is activated constitutively in Hep3B cells, as determined by electrophoretic mobility shift assays ; 2 ) RelA/NF-kappaB reporter gene activity is inhibited specifically by dominant negative mutants of IkappaB(alpha), IKK1, IKK2, MEKK1 , and MEKK3 and it is activated by overexpression of wild-type MEKK3, suggesting that upstream kinase cascades induce phosphorylation of IkappaB(alpha) and activate RelA/NF-kappaB in Hep3B cells ; 3 ) overexpression of the HBV x gene fails to activate NF-kappaB in HepG2 and Chang cell lines ; 4 ) The NF-kappaB-inducible gene, bcl-xl, is overexpressed in Hep3B cells and is inhibited by the proteosome inhibitor PS341, which prevents IkappaBalpha degradation and RelA/NF-kappaB activation ; and 5 ) inhibition of constitutive RelA/NF-kappaB activity by PS341 sensitizes Hep3B cells to doxorubicin induced apoptosis ... The current study data demonstrate that 1 ) RelA/NF-kappaB activity is activated constitutively in Hep3B cells, as determined by electrophoretic mobility shift assays ; 2 ) RelA/NF-kappaB reporter gene activity is inhibited specifically by dominant negative mutants of IkappaB(alpha), IKK1, IKK2, MEKK1, and MEKK3 and it is activated by overexpression of wild-type MEKK3, suggesting that upstream kinase cascades induce phosphorylation of IkappaB(alpha) and activate RelA/NF-kappaB in Hep3B cells ; 3 ) overexpression of the HBV x gene fails to activate NF-kappaB in HepG2 and Chang cell lines ; 4 ) The NF-kappaB-inducible gene, bcl-xl, is overexpressed in Hep3B cells and is inhibited by the proteosome inhibitor PS341, which prevents IkappaBalpha degradation and RelA/NF-kappaB activation ; and 5 ) inhibition of constitutive RelA/NF-kappaB activity by PS341 sensitizes Hep3B cells to doxorubicin induced apoptosis ... The current study data demonstrate that 1 ) RelA/NF-kappaB activity is activated constitutively in Hep3B cells, as determined by electrophoretic mobility shift assays ; 2 ) RelA/NF-kappaB reporter gene activity is inhibited specifically by dominant negative mutants of IkappaB(alpha) , IKK1, IKK2, MEKK1, and MEKK3 and it is activated by overexpression of wild-type MEKK3, suggesting that upstream kinase cascades induce phosphorylation of IkappaB(alpha) and activate RelA/NF-kappaB in Hep3B cells ; 3 ) overexpression of the HBV x gene fails to activate NF-kappaB in HepG2 and Chang cell lines ; 4 ) The NF-kappaB-inducible gene, bcl-xl, is overexpressed in Hep3B cells and is inhibited by the proteosome inhibitor PS341, which prevents IkappaBalpha degradation and RelA/NF-kappaB activation ; and 5 ) inhibition of constitutive RelA/NF-kappaB activity by PS341 sensitizes Hep3B cells to doxorubicin induced apoptosis
Berin et al., Cell Microbiol 2002 (MAP Kinase Signaling System) : Isogenic eae- and stx- mutants also activated p38 and ERK MAP kinases, and NF-kappaB and stimulated increases in IL-8 protein secretion similar to those of wild-type EHEC
Ares et al., BMC immunology 2002 : In contrast, oxidized LDL stimulated AP-1 and PPARgamma but inhibited NF-kappaB , indicating that the effects of lipid loading with ac-LDL were not due to oxidation of lipids ... In contrast, oxidized LDL stimulated AP-1 and PPARgamma but inhibited NF-kappaB , indicating that the effects of lipid loading with ac-LDL were not due to oxidation of lipids ... In contrast, oxidized LDL stimulated AP-1 and PPARgamma but inhibited NF-kappaB , indicating that the effects of lipid loading with ac-LDL were not due to oxidation of lipids
Quivy et al., J Virol 2002 (HIV Infections) : Here, we demonstrate that TSA ( NaBut ) synergized with both ectopically expressed p50/p65 and tumor necrosis factor alpha/SF2 (TNF) induced NF-kappaB to activate the LTR
Yu et al., J Immunol 2002 : Ectopic pellino-2 expression did not result in NF-kappaB activation ... However, ectopic expression of a mouse pellino-2 antisense construct inhibited IL-1 or LPS induced activation of NF-kappaB dependent IL-8 promoter activity
Gumucio et al., Clin Exp Rheumatol 2002 (Autoimmune Diseases...) : ASC itself participates in at least three important cellular processes : apoptosis, recruitment and activation of pro-caspase-1 ( with associated processing and secretion of IL-1beta ), and activation of NF-kappaB ( a transcription factor involved in both initiation and resolution of the inflammatory response )
Lee et al., Biochem Biophys Res Commun 2002 : Electromobility shift assays showed that the expression of p65 induced the binding of the p65/p65 homodimer to the NF-kappaB site at -1180
Cominacini et al., Am J Hypertens 2002 : In this study we have compared the effects of two different angiotensin converting enzyme (ACE) inhibitors, one possessing an active sulfhydryl group ( zofenopril ) and one lacking this group ( enalapril ) on the cellular redox state ( monitored by measuring intracellular reactive oxygen species and thiol status ), expression of adhesion molecules, and activation of NF-kappaB in human umbilical vein endothelial cells ( HUVECs ) ... Ox-LDL and TNF-alpha increased the activation of NF-kappaB and the preincubation of HUVECs with zofenoprilat, but not with enalaprilat, dose dependently reduced its activation ( P < .001 ) ... Ox-LDL and TNF-alpha increased the activation of NF-kappaB and the preincubation of HUVECs with zofenoprilat, but not with enalaprilat, dose dependently reduced its activation ( P < .001 )
Shibuya et al., Biochem Biophys Res Commun 2002 : PLL-g-HA/NF-kappaB31, but not control oligodeoxynucleotides having a reverse or scrambled sequence, inhibited the intranuclear localization of NF-kappaB induced by TNFalpha , with almost complete inhibition at 2.5microg/mL as DNA
Muroi et al., Infect Immun 2002 : The polysaccharide portion plays an indispensable role in Salmonella lipopolysaccharide induced activation of NF-kappaB through human toll-like receptor 4 ... Thus we investigated the critical role of polysaccharide in Salmonella LPS induced activation of NF-kappaB ... These results indicate that the polysaccharide portion covalently bound to lipid A plays the principal role in Salmonella LPS induced activation of NF-kappaB through human CD14/TLR4/MD-2 ... These results indicate that the polysaccharide portion covalently bound to lipid A plays the principal role in Salmonella LPS induced activation of NF-kappaB through human CD14/TLR4/MD-2 ... These results indicate that the polysaccharide portion covalently bound to lipid A plays the principal role in Salmonella LPS induced activation of NF-kappaB through human CD14/TLR4/MD-2
Borgmann et al., Endothelium : journal of endothelial cell research 2002 : Alteration of cytokine release was accompanied by reduced basal and TNF-alpha stimulated nuclear factor-kappa B (NF-kappa B) and activator protein-1 (AP-1) activity
Tsunoda et al., Cancer Res 2002 : We report here that full-length Mig-6 , but not CRIB domain deleted Mig-6 ( DeltaMig-6 ) or uncleavable mutant of Mig-6 ( Mig-6-S38A ), induces transcriptional activation of nuclear factor of kappaB (NFkappaB) , which is inhibited by inhibitor of kappaBalpha ( IkappaBalpha ), and that the processed NH ( 2 ) -terminal region of Mig-6 but not the full length is bound with IkappaBalpha through its NFkappaB binding region
Aksoy et al., Eur J Immunol 2002 : Furthermore, we found that PKC inhibition impaired LPS induced I kappa B-alpha degradation and subsequent nuclear factor (NF)-kappa B activation in DC
Tonon et al., Eur J Immunol 2002 : Secondly, studies on DC generated in vitro by culturing monocytes with IL-4 and GM-CSF showed that PTX directly stimulates MHC class II and costimulatory molecules up-regulation, cytokine synthesis and NF-kappa B activation
Dejardin et al., Immunity 2002 : This activation of NF-kappaB2 requires NIK and IKKalpha , while NEMO/IKKgamma is dispensable for p100 processing ... IKKbeta dependent activation of canonical NF-kappaB is required for the expression but not processing of p100 and for the expression of proinflammatory molecules including VCAM-1, MIP-1beta , and MIP-2 in response to LTbetaR ligation ... IKKbeta dependent activation of canonical NF-kappaB is required for the expression but not processing of p100 and for the expression of proinflammatory molecules including VCAM-1, MIP-1beta, and MIP-2 in response to LTbetaR ligation ... IKKbeta dependent activation of canonical NF-kappaB is required for the expression but not processing of p100 and for the expression of proinflammatory molecules including VCAM-1 , MIP-1beta, and MIP-2 in response to LTbetaR ligation ... IKKbeta dependent activation of canonical NF-kappaB is required for the expression but not processing of p100 and for the expression of proinflammatory molecules including VCAM-1, MIP-1beta, and MIP-2 in response to LTbetaR ligation ... IKKbeta dependent activation of canonical NF-kappaB is required for the expression but not processing of p100 and for the expression of proinflammatory molecules including VCAM-1, MIP-1beta , and MIP-2 in response to LTbetaR ligation ... IKKbeta dependent activation of canonical NF-kappaB is required for the expression but not processing of p100 and for the expression of proinflammatory molecules including VCAM-1, MIP-1beta, and MIP-2 in response to LTbetaR ligation
Yoo et al., Biochem Biophys Res Commun 2002 : Electrophoretic mobility shift assay and site directed mutagenesis study on the consensus binding site for NF-kappaB revealed that the activation of NF-kappaB is required for the IL-1beta induced activation of MMP-9 promoter
Grenier et al., FEBS Lett 2002 : Co-expression of PYPAF5 with ASC results in a synergistic activation of NF-kappaB and the recruitment of PYPAF5 to punctate structures in the cytoplasm
Russell et al., Mol Biol Cell 2002 (Glioblastoma) : In contrast to nuclear factor-kappaB (NF-kappaB) activation by tumor necrosis factor-alpha (TNF-alpha) , the specific processes involved in the activation of this transcription factor by ionizing radiation ( IR ) have not been completely defined
Prasad et al., J Lab Clin Med 2002 : Using transfection of expression vectors of anti-sense NF-kappaB p105 ( precursor of NF-kappaB p50 ) in cells, we showed that a decrease in gene expression of IL-2 and IL-2 Ralpha may be partly due to decreased activation of NF-kappaB in zinc-deficient cells
Cárcamo et al., Biochemistry 2002 : Vitamin C suppresses TNF alpha induced NF kappa B activation by inhibiting I kappa B alpha phosphorylation ... Tumor necrosis factor-alpha (TNFalpha) activates NFkappaB via a well defined kinase pathways involving NFkappaB inducing kinase (NIK) , which activates downstream multisubunit IkappaB kinases (IKK) ... Tumor necrosis factor-alpha (TNFalpha) activates NFkappaB via a well defined kinase pathways involving NFkappaB inducing kinase (NIK), which activates downstream multisubunit IkappaB kinases (IKK) ... We found that intracellular vitamin C inhibits TNFalpha induced activation of NFkappaB in human cell lines ( HeLa, monocytic U937, myeloid leukemia HL-60, and breast MCF7 ) and primary endothelial cells ( HUVEC ) in a dose dependent manner ... Vitamin C-loaded cells showed significantly decreased TNFalpha induced nuclear translocation of NFkappaB , NFkappaB dependent reporter transcription, and IkappaBalpha phosphorylation
Ayroldi et al., Mol Cell Biol 2002 (MAP Kinase Signaling System) : In fact, GILZ overexpression inhibits TCR activated NF-kappaB nuclear translocation, interleukin-2 production, FasL upregulation, and the consequent activation induced apoptosis
Medvedev et al., J Immunol 2002 : Pretreatment of human monocytes with LPS decreased LPS mediated NF-kappaB activation, p38 mitogen activated protein kinase phosphorylation, and TNF-alpha gene expression, documenting the induction of endotoxin tolerance
Coward et al., J Immunol 2002 : The possible participation of TNF-alpha generated by mast cells in NF-kappaB activation by anti-IgE was investigated using a blocking Ab for TNF-alpha
Pei et al., Cytokine 2002 : Asbestos exposure and particulate air pollution have been reported to stimulate the expression of IL-8 and to induce NF-kappaB activation ... We have assessed the effect of adenovirus mediated overexpression of the NF-kappaB inhibitor, IkappaBalpha on PAH induced IL-8 expression in A549 cell line ... This suggests that PAH induced IL-8 gene regulation may be mediated by NF-kappaB ... These data indicate that PAH alone, either B [ a ] P or 1-NP, is sufficient to stimulate NF-kappaB activation and IL-8 transcription
Andreakos et al., Blood 2003 : One such signal is likely to be CD40 ligand (CD40L), as IKK2dn blocked CD40L but not lipopolysaccharide (LPS) induced NF-kappaB activation, cytokine production, and up-regulation of costimulatory molecules and HLA-DR in DCs
Kleemann et al., Blood 2003 : First, PPAR-alpha increases IkappaB-alpha expression and thus prevents p50-NFkappaB translocation to the nucleus ... First, PPAR-alpha increases IkappaB-alpha expression and thus prevents p50-NFkappaB translocation to the nucleus ... Our findings identify C/EBP-beta and p50-NFkappaB as novel targets for PPAR-alpha and provide a molecular explanation for the reduction of plasma CRP levels by fibrates
Verrecchia et al., EMBO Rep 2002 : Conversely, in NF-kappaB essential modulator-/- ( NEMO-/- ) fibroblasts, lack of NF-kappaB activation did not influence the antagonism exerted by TNF-alpha against TGF-beta but prevented repression of basal COL1A2 gene expression
Deng et al., Cancer Cell 2002 (Breast Neoplasms...) : beta-catenin interacts with and inhibits NF-kappa B in human colon and breast cancer ... Importantly, activated beta-catenin was found to inhibit the expression of NF-kappa B target genes, including Fas and TRAF1 ... Furthermore, a strong inverse correlation was identified between the expression levels of beta-catenin and Fas in colon and breast tumor tissues, suggesting that beta-catenin regulates NF-kappa B and its targets in vivo
Lee et al., Nephron 2002 : SIN-1 dose dependently inhibited the TNF-alpha- or IL-1beta induced NF-kappaB binding activity and suppressed the TNF-alpha induced degradation of IkappaB-alpha
Wang et al., Cancer 2002 (Carcinoma...) : The results showed that TNF-alpha can induce activation of NF-kappaB and that the activation and translocation of NF-kappaB into the nucleus is responsible for promoting the 3-D cytomorphologic differentiation of anaplastic thyroid carcinoma cells, which was inhibited by the NF-kappaB translocation inhibitor, NF-kappaB SN50 ... The current data suggest that TNF-alpha can induce thyrocyte differentiation in anaplastic thyroid carcinoma cells through NF-kappaB and that it merits investigation as differentiation therapy for the treatment of patients with anaplastic thyroid carcinoma
Ogawa et al., Int Immunol 2002 : P. gingivalis LPS and its natural lipid A fraction induced NF-kappa B activation primarily in Ba/F3 cells expressing mouse TLR 2 ( Ba/mTLR2 ), rather than in those expressing mouse TLR4 and its accessory protein MD2 ( Ba/mTLR4/mMD2 )
Dijkstra et al., Scand J Gastroenterol Suppl 2002 (Inflammatory Bowel Diseases) : Review of the literature on NF-kappaB activation and iNOS induction in IBD ... For example, inhibition of the NF-kappaB mediated induction of iNOS in epithelial cells could block important anti-apoptotic and anti-microbial survival mechanisms
Ishii et al., Toxicol Appl Pharmacol 2002 : These results suggest that BLM can directly induce E-selectin expression with an increase in transcription in endothelial cells through activation and nuclear translocation of NF-kappaB/Rel without mediation of inflammatory cytokines ... These results suggest that BLM can directly induce E-selectin expression with an increase in transcription in endothelial cells through activation and nuclear translocation of NF-kappaB/Rel without mediation of inflammatory cytokines
Condorelli et al., FASEB J 2002 : Here we demonstrate in neonatal rat cardiomyocytes that 1 ) TNF-alpha induces phosphorylation of AKT, activation of NF-kappaB, and the phosphorylation of JUN kinase ; 2 ) blocking AKT activity prevents NF-kappaB activation, suggesting a role for AKT in regulating NF-kappaB function ; 3 ) AKT and JUN are both critical for the hypertrophic effects of TNF-alpha, since dominant negative mutants of these genes are capable of inhibiting TNF-alpha induced ANF-promoter up-regulation and increase in cardiomyocyte cell size, and 4 ) blocking NF-kappaB, AKT, or JUN alone or in combination does not sensitize cardiomyocytes to the proapoptotic effects of TNF-alpha, in contrast to other cell types, suggesting a cardiac-specific pathway regulating the anti-apoptotic events induced by TNF-alpha
Shimada et al., Mol Carcinog 2002 (MAP Kinase Signaling System...) : We found that NFkappaB activation inhibited c-jun NH ( 2 ) -terminal kinase and caspase 3 activation induced by 4-HPR and that NFkappaB inhibition by the I kappa B alpha phosphorylation inhibitor compound Bay 117082 resulted in increasing sensitization of both PC-3 and DU145 lines to apoptosis induced by 4-HPR at low concentrations
Habelhah et al., EMBO J 2002 : Siah2 efficiently decreases TNF-alpha dependent induction of JNK activity and transcriptional activation of NF-kappaB
Kato et al., Mol Immunol 2002 : The IL-6 production by human fibroblasts in response to these cystatins was inhibited by tyrosine kinase inhibitors and an inhibitor of NF-kappa B activation ... These findings supply evidence that cystatins SA1 and SA2 adhere to human fibroblasts and that the event results in tyrosine phosphorylation and upregulation of the release of IL-6 mediated enhancement of NF-kappa B activity
Wakisaka et al., Cancer Res 2002 : These results suggest that FGF-2 release is independently mediated by NF-kappaB signaling, not simply a consequence of induction itself ... Thus, NF-kappaB signaling is involved in induction of expression and release of FGF-2 by LMP1
Yang et al., Life Sci 2002 : Treatment of cells with sphondin ( 50 microM ) or the NF-kappaB inhibitor, PDTC ( 50 microM ) partially inhibited IL-1beta induced degradation of IkappaB-alpha in the cytosol and translocation of p65 NF-kappaB from the cytosol to the nucleus ... Furthermore, IL-1beta induced NF-kappaB-specific DNA-protein complex formation in the nucleus was partially inhibited by sphondin ( 50 microM ) or PDTC ( 50 microM ) ... Furthermore, IL-1beta induced NF-kappaB-specific DNA-protein complex formation in the nucleus was partially inhibited by sphondin ( 50 microM ) or PDTC ( 50 microM )
Waris et al., Biochem Pharmacol 2002 (Hepatitis C) : The inhibitory effect of protein tyrosine kinase ( PTK ) inhibitors indicates the involvement of PTK in NF-kappaB activation by NS5A
Sur et al., Biochem Pharmacol 2002 : Transient transfection assays using wild-type and mutant NOS2 promoter/luciferase reporter constructs showed that DNA binding of the transcription factors Stat1 and NF-kappaB was essential for optimal expression of the NOS2 gene
Yanagisawa et al., Biochem J 2003 : However, IRAK-1-S overexpressed in 293T cells induced constitutive activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK) independent of stimulation by IL-1, as did IRAK-1-W ... These results also indicate that this novel variant may play roles in the activation of NF-kappaB and JNK by IL-1 and other ligands whose signal transduction is dependent on IRAK-1 under physiological conditions
Fu et al., J Biol Chem 2003 : Our data support the notion that IKK gamma associated PP2A is responsible for the rapid deactivation of IKK, and inhibition of PP2A by Tax in the context of IKK x PP2A x Tax ternary complex leads to constitutive IKK and NF-kappa B activation
Omori et al., Free radical research 2002 : TNF-alpha stimulation also induced marked activation of nuclear factor KB ( NF-kappaB )
Tomita et al., Respirology 2002 : Using a human airway cell line ( LC-2/ad ), lipopolysaccharide (LPS) induced gene expression of hBD-2 was studied in the absence or the presence of ( i ) dexamethasone, ( ii ) inhibition of NF-kappaB and AP-1, ( iii ) intracellular calcium chelator, and ( iv ) cyclooxygenase (COX) inhibitors ... Lipopolysaccharide induced gene expression of hBD-2 was down-regulated by ( i ) dexamethasone, ( ii ) inhibition of NF-kappaB and AP-1, and ( iii ) intracellular calcium chelator ... These findings suggest that glucocorticoids ( GC ), but not COX inhibitors, reduce hBD-2 gene expression, while NF-kappaB , AP-1 and intracellular calcium are essential for hBD-2 expression ... These findings suggest that glucocorticoids ( GC ), but not COX inhibitors, reduce hBD-2 gene expression, while NF-kappaB , AP-1 and intracellular calcium are essential for hBD-2 expression
Pinteaux et al., J Neurochem 2002 : Bacterial lipopolysaccharide (LPS) caused increased expression of IL-1RI, IL-1RII and IL-1RAcP mRNAs, induced the release of IL-1beta , IL-6 and prostaglandin-E2 ( PGE2 ), and activated nuclear factor kappaB (NF-kappaB) and the mitogen activated protein kinases ( MAPKs ) p38, and extracellular signal regulated protein kinase ( ERK1/2 ), but not c-Jun N-terminal kinase (JNK) in microglial cultures ... Bacterial lipopolysaccharide (LPS) caused increased expression of IL-1RI, IL-1RII and IL-1RAcP mRNAs, induced the release of IL-1beta, IL-6 and prostaglandin-E2 ( PGE2 ), and activated nuclear factor kappaB (NF-kappaB) and the mitogen activated protein kinases ( MAPKs ) p38, and extracellular signal regulated protein kinase ( ERK1/2 ), but not c-Jun N-terminal kinase (JNK) in microglial cultures ... Bacterial lipopolysaccharide (LPS) caused increased expression of IL-1RI, IL-1RII and IL-1RAcP mRNAs, induced the release of IL-1beta, IL-6 and prostaglandin-E2 ( PGE2 ), and activated nuclear factor kappaB (NF-kappaB) and the mitogen activated protein kinases ( MAPKs ) p38, and extracellular signal regulated protein kinase ( ERK1/2 ), but not c-Jun N-terminal kinase (JNK) in microglial cultures ... In comparison, IL-1beta induced the release of PGE2, IL-6 and activated NF-kappaB , p38, JNK and ERK1/2 in mixed glial cultures, but failed to induce any of these responses in microglial cell cultures ... In comparison, IL-1beta induced the release of PGE2, IL-6 and activated NF-kappaB , p38, JNK and ERK1/2 in mixed glial cultures, but failed to induce any of these responses in microglial cell cultures
Savaskan et al., FASEB J 2003 (Disease Susceptibility...) : Gel shift analysis demonstrates that this effect is connected to the inhibition of glutamate induced NF-kappaB and AP-1 activation
Ramesh et al., Toxicol Lett 2002 : Activation of NF-kappaB DNA binding activity by Mn ( 2+ ) at 1.0 microM correlated with proteolytic degradation of the inhibitory subunit IkappaB(alpha) as evidenced in cytosol
Huang et al., Environ Health Perspect 2002 : We also investigated the role of NF-kappaB in the induction of Cap43 by nickel compounds using dominant negative mutant Ikappabeta kinase b-KM BEAS-2B transfectants
Jiang et al., Arterioscler Thromb Vasc Biol 2002 (MAP Kinase Signaling System) : Treatment of VSMCs with PDGF or EGF alone potently induced ERK phosphorylation and DNA synthesis but did not induce NF-kappaB activation or iNOS expression ... However, either PDGF or EGF markedly enhanced interleukin-1beta induced persistent NF-kappaB activation and iNOS expression but did not affect the early and transient NF-kappaB activation ... However, either PDGF or EGF markedly enhanced interleukin-1beta induced persistent NF-kappaB activation and iNOS expression but did not affect the early and transient NF-kappaB activation ... Inhibition of ERK phosphorylation with selective inhibitors ( PD98059 or U0126 ) attenuated interleukin-1beta induced persistent NF-kappaB activation and iNOS expression in either the absence or presence of the growth factors ... Inhibition of ERK phosphorylation with selective inhibitors ( PD98059 or U0126 ) attenuated interleukin-1beta induced persistent NF-kappaB activation and iNOS expression in either the absence or presence of the growth factors ... These results indicate that interleukin-1beta induced expression of NF-kappaB dependent genes, such as iNOS, is potentiated in the presence of growth factors through a mechanism requiring ERK dependent enhanced NF-kappaB activation, and the results also suggest that NF-kappaB activation is not required for PDGF or EGF to trigger DNA synthesis in VSMCs ... These results indicate that interleukin-1beta induced expression of NF-kappaB dependent genes, such as iNOS, is potentiated in the presence of growth factors through a mechanism requiring ERK dependent enhanced NF-kappaB activation , and the results also suggest that NF-kappaB activation is not required for PDGF or EGF to trigger DNA synthesis in VSMCs
Burr et al., Exp Brain Res 2002 : However, activated NF-kappaB levels were reduced by a guanylate cyclase inhibitor, the Src-family tyrosine kinase inhibitor PP1 , or the farnesyl transferase inhibitors manumycin and farnesyl transferase ( Ftase ) inhibitor 1
Iordanskiy et al., Virology 2002 : Instead, PTX-B activity is mediated through suppression of the cellular transcription factor, NF-kappaB
Uzzo et al., Clin Cancer Res 2002 (Prostatic Neoplasms) : In this study, we demonstrate by immunoblotting and immunohistochemistry that physiological levels of zinc inhibit activation of nuclear factor (NF)-kappa B transcription factor in PC-3 and DU-145 human prostate cancer cells, reduce expression of NF-kappa B-controlled antiapoptotic protein c-IAP2 , and activate c-Jun NH ( 2 ) -terminal kinases ... In this study, we demonstrate by immunoblotting and immunohistochemistry that physiological levels of zinc inhibit activation of nuclear factor (NF)-kappa B transcription factor in PC-3 and DU-145 human prostate cancer cells, reduce expression of NF-kappa B-controlled antiapoptotic protein c-IAP2 , and activate c-Jun NH ( 2 ) -terminal kinases
Fan et al., J Biol Chem 2003 (Anoxia) : In the present study, we investigated the involvement of c-Src in the redox activation of NFkappaB following H/R or pervanadate treatment ... Overexpression of glutathione peroxidase-1 or catalase, but not Mn-SOD or Cu, Zn-SOD, significantly reduced both NF kappa B activation and tyrosine phosphorylation of I kappa B alpha ... Overexpression of glutathione peroxidase-1 or catalase , but not Mn-SOD or Cu, Zn-SOD, significantly reduced both NF kappa B activation and tyrosine phosphorylation of I kappa B alpha ... Overexpression of glutathione peroxidase-1 or catalase, but not Mn-SOD or Cu, Zn-SOD, significantly reduced both NF kappa B activation and tyrosine phosphorylation of I kappa B alpha ... These results suggest that c-Src dependent tyrosine phosphorylation of I kappa B alpha and subsequent activation of NF kappa B is controlled by intracellular H ( 2 ) O ( 2 ) and defines an important redox regulated pathway for NF kappa B activation following H/R injury that is independent of the IKK complex ... These results suggest that c-Src dependent tyrosine phosphorylation of I kappa B alpha and subsequent activation of NF kappa B is controlled by intracellular H ( 2 ) O ( 2 ) and defines an important redox regulated pathway for NF kappa B activation following H/R injury that is independent of the IKK complex
Ladner et al., J Biol Chem 2003 (Cachexia) : We propose that the biphasic activation of NF-kappaB in response to TNF may play a key regulatory role in skeletal muscle wasting associated with cachexia
Haddad et al., Int Immunopharmacol 2002 (Translocation, Genetic) : BCNU and BSO reduced LPS induced NF-kappaB activation as determined by the electrophoretic mobility shift DNA binding assay
Kinjyo et al., Immunity 2002 : LPS induced I-kappaB and p38 phosphorylation was upregulated in SOCS1 ( -/- ) macrophages, and forced expression of SOCS1 suppressed LPS induced NF-kappaB activation ... LPS induced I-kappaB and p38 phosphorylation was upregulated in SOCS1 ( -/- ) macrophages, and forced expression of SOCS1 suppressed LPS induced NF-kappaB activation
Nakagawa et al., Immunity 2002 (Gram-Negative Bacterial Infections...) : Introduction of SOCS-1 inhibited LPS induced NF-kappaB and STAT1 activation in macrophages
Cogswell et al., J Biol Chem 2003 : Stimulation of cells with inflammatory cytokines such as tumor necrosis factor alpha or interleukin-1 induces the degradation of I kappa B, the inhibitor of NF-kappa B , allowing nuclear accumulation of NF-kappa B and regulation of specific gene expression ... Inhibition of activation of mitochondrial NF-kappa B by expression of the superrepressor form of I kappa B alpha inhibited the loss of expression of both cytochrome c oxidase III and cytochrome b mRNA ... Inhibition of activation of mitochondrial NF-kappa B by expression of the superrepressor form of I kappa B alpha inhibited the loss of expression of both cytochrome c oxidase III and cytochrome b mRNA
Huang et al., FEBS Lett 2002 : Human T-cell leukemia virus type 1 oncoprotein Tax activates NF-kappaB through direct binding to IKK-gamma, the regulatory component of the IkappaB kinase complex ... Taken together, our findings suggest a new model of Tax activation of NF-kappaB , in which Tax interacts with IKK-gamma to stimulate its oligomerization
Chinanonwait et al., FEBS Lett 2002 : Our results suggested that lack of a negative regulation of IKK could lead to permanent NF-kappaB activation
Casciati et al., J Neurochem 2002 (Amyotrophic Lateral Sclerosis...) : In this paper, we report a calcineurin dependent activation of nuclear factor-kappaB (NF-kappaB) induced by the expression of familial amyotrophic lateral sclerosis ( fALS ) -SOD1s in human neuroblastoma cell lines
Wang et al., J Neurochem 2002 (Neuroblastoma) : PGA1 blocked the rotenone induced nuclear translocation of NF-kappaB and attenuated, but did not abolish, the caspase-3 elevation
Romieu-Mourez et al., Cancer Res 2002 (Breast Neoplasms...) : Surprisingly, the IkappaB kinase (IKK) kinase complex, implicated in proteasome mediated degradation of IkappaB-alpha and activation of NF-kappaB via the canonical pathway, was not activated in these cells ... In NIH 3T3 fibroblasts, which express low basal NF-kappaB and CK2 activities, overexpression of CK2 by retroviral gene delivery led to increased IkappaB-alpha turnover and the induction of classical NF-kappaB ( p50/RelA )
Nakata et al., Kekkaku 2002 (HIV Infections...) : Reduction of expression of an inhibitory C/EBP beta transcription factor and activation of NF-kappa B are observed at the site of inflammation ... Reduction of expression of an inhibitory C/EBP beta transcription factor and activation of NF-kappa B are observed at the site of inflammation
Masuda et al., J Exp Ther Oncol 2002 (Breast Neoplasms...) : An analysis of alternative pathways indicated that EGCG strongly inhibited the constitutive activation of NF-kappa B in both cell lines, and an NF-kappa B inhibitor strongly inhibited VEGF production ... These results suggest that EGCG inhibits VEGF production by inhibiting both the constitutive activation of Stat3 and NF-kappa B , but not extracellular-signal regulated kinase ( ERK ) or Akt, in these cells
Fukuda et al., Virology 2002 (Cell Transformation, Neoplastic...) : EBV latent membrane protein 1 (LMP1) induced constitutive ERK 1/2 phosphorylation and NF-kappa B activation in LMP1 transfected HSC-39 cells, which then became resistant to TGF-beta 1-mediated growth inhibition, TGF-beta 1-mediated ERK 1/2 phosphorylation, and p21 induction, and proliferated in low-serum medium
Umezawa et al., Mol Cells 2002 : The designed compound, dehydroxymethyl-epoxyquinomicin ( DHMEQ ), inhibited the TNF-alpha induced activation of NF-kappaB , and showed an anti-arthritic effect in mice ... DHMEQ inhibited the TNF-alpha induced cellular DNA binding of nuclear NF-kappaB , but not the phosphorylation or degradation of I-kappaB
Sarady et al., Am J Respir Cell Mol Biol 2002 : CO inhibited LPS induced activation of NF-kappaB , which has been shown to regulate GM-CSF transcription, by preventing the phosphorylation and degradation of the regulatory subunit IkappaB-alpha
Tolnay et al., J Immunol 2002 : Here we show that NF-kappaB enhances the expression of the human CR2 gene ... We propose that the NF-kappaB signaling pathway enhances the expression of the CR2 gene, as a result of NF-kappaB proteins binding to two CR2 promoter elements
Marrache et al., J Immunol 2002 : COX-2 expression was prevented by mitogen activated protein kinase kinase/extracellular signal regulated kinase 1/2 and NF-kappaB inhibitors
Ashikawa et al., J Immunol 2002 : Piceatannol inhibits TNF induced NF-kappaB activation and NF-kappaB mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation ... The treatment of human myeloid cells with piceatannol suppressed TNF induced DNA binding activity of NF-kappaB ... The effect of piceatannol was not restricted to myeloid cells, as TNF induced NF-kappaB activation was also suppressed in lymphocyte and epithelial cells ... Piceatannol abrogated the expression of TNF induced NF-kappaB dependent reporter gene and of matrix metalloprotease-9, cyclooxygenase-2, and cyclin D1 ... Piceatannol abrogated the expression of TNF induced NF-kappaB dependent reporter gene and of matrix metalloprotease-9, cyclooxygenase-2 , and cyclin D1
Preston et al., J Am Soc Nephrol 2002 : Novel effects of neutrophil derived proteinase 3 and elastase on the vascular endothelium involve in vivo cleavage of NF-kappaB and proapoptotic changes in JNK, ERK, and p38 MAPK signaling pathways
Nys et al., Clin Sci (Lond) 2002 (Bronchopneumonia...) : NF-kappaB activation by MPO was also demonstrated
Neznanov et al., J Biol Chem 2003 : These data indicate that SIRPalpha/SHPS-1 is involved in negative regulation of NF-kappaB signaling
Tang et al., Mol Cell Biol 2002 (MAP Kinase Signaling System) : The absence of NF-kappaB mediated inhibition of c-Jun N-terminal kinase activation contributes to tumor necrosis factor alpha induced apoptosis
Yamamoto et al., Nature 2002 : As in MyD88-deficient mice, LPS activation of the nuclear factor NF-kappaB and mitogen activated protein kinases is induced with delayed kinetics in TIRAP-deficient mice
Kim et al., Hepatology 2002 (Carcinoma, Hepatocellular...) : TRAIL induced NF-kappaB activation was preceded by IkappaBalpha kinase (IKK) activation and IkappaBalpha degradation and depended on TRAF2, NF-kappaB inducing kinase (NIK), IKK1, and IKK2
Seitzer et al., J Cell Physiol 2003 (Bacterial Infections...) : These experiments allow the conclusion that LPS in the cytoplasmic compartment does not lead to NFkappaB translocation, cytokine mRNA transcription, and NFkappaB dependent protein expression and suggest that these activation parameters require the interaction of LPS with external membrane components
Okuda et al., Nihon Shinkei Seishin Yakurigaku Zasshi 2002 : Neuroprotection by NMDA is mediated by a de novo synthesis of proteins such as brain derived neurotrophic factor , whose expression is activated by the transcription factor NF kappa B ... Neuroprotection by NMDA is mediated by a de novo synthesis of proteins such as brain derived neurotrophic factor , whose expression is activated by the transcription factor NF kappa B
Prösch et al., Biol Chem 2002 : Construction and testing of a series of promoter mutants demonstrated that NF-kappaB is essential for both TNFalpha and IE1 stimulation ... Specific inhibition of NF-kappaB signalling by co-expression of a dominant negative IkappaB variant reduced TNFalpha stimulation of the IE1/2 enhancer/promoter by up to 80 %
Morcos et al., Diabetes 2002 (Diabetes Mellitus, Type 2...) : To further prove an AGE/CML induced NF-kappaB activation in pTECs, NF-kappaB activation was studied in cultured human pTECs by electrophoretic mobility shift assays ( EMSAs ) and Western blot ... The AGE- and CML dependent activation of NF-kappaBp65 and NF-kappaB dependent IL-6 expression could be inhibited using the soluble form of the receptor for AGEs (RAGE) ( soluble RAGE [ sRAGE ] ), RAGE-specific antibody, or the antioxidant thioctic acid ... The AGE- and CML dependent activation of NF-kappaBp65 and NF-kappaB dependent IL-6 expression could be inhibited using the soluble form of the receptor for AGEs (RAGE) ( soluble RAGE [ sRAGE ] ), RAGE-specific antibody, or the antioxidant thioctic acid ... The findings that excreted pTECs demonstrate activated NF-kappaB and IL-6 antigen and that AGE-albumin and CML lead to a perpetuated activation of NF-kappaB in vitro infer that a perpetuated increase in proinflammtory gene products, such as IL-6, plays a role in damaging the renal tubule ... The findings that excreted pTECs demonstrate activated NF-kappaB and IL-6 antigen and that AGE-albumin and CML lead to a perpetuated activation of NF-kappaB in vitro infer that a perpetuated increase in proinflammtory gene products, such as IL-6, plays a role in damaging the renal tubule
Potter et al., J Biol Chem 2003 : LPS increased NF-kappaB and USF binding to the ADH promoter
Chen et al., Biorheology 2003 (Mechanotransduction, Cellular) : The induction of IL-8 gene expression by laminar shear stress is probably due to the activation of NF-kappaB
Greten et al., Gastroenterology 2002 (Carcinoma, Ductal, Breast...) : Functional analysis shows that blocking of both Stat3 and NF-kappaB together induces programmed cell death in murine pancreatic tumor cells
Krishnaswamy et al., Frontiers in bioscience : a journal and virtual library 2003 (Leukemia, Mast-Cell) : The NF-kappaB inhibitor, Bay-11, inhibited expression of MCP-1
Saitoh et al., FEBS Lett 2002 : Lymphotoxin-beta receptor mediates NEMO independent NF-kappaB activation
Zhang et al., FEBS Lett 2002 : Co-expression of the alphav-TLR and beta5-TLR chimeras in 293T cells generated 10 TLR homodimers, but only TLR4/4 could effectively activate NF-kappaB ... NF-kappaB activation by TLR2/1, TLR2/6 and the TLR4 monomer, but not TLR4/4, was completely inhibited by dominant negative MyD88 , suggesting that TLR4 homodimers and monomers could activate NF-kappaB through different mechanisms ... NF-kappaB activation by TLR2/1, TLR2/6 and the TLR4 monomer, but not TLR4/4, was completely inhibited by dominant negative MyD88, suggesting that TLR4 homodimers and monomers could activate NF-kappaB through different mechanisms
Kanamori et al., FEBS Lett 2002 : Act1 mediated NF-kappaB activation was inhibited by a dominant negative mutant of TRAF6 in a dose dependent manner, and IL-1 induced NF-kappaB activation was inhibited by a high level of Act1 expression ... Act1 mediated NF-kappaB activation was inhibited by a dominant negative mutant of TRAF6 in a dose dependent manner, and IL-1 induced NF-kappaB activation was inhibited by a high level of Act1 expression ... Act1 mediated NF-kappaB activation was inhibited by a dominant negative mutant of TRAF6 in a dose dependent manner, and IL-1 induced NF-kappaB activation was inhibited by a high level of Act1 expression
Yu et al., Neurobiol Dis 2002 (Necrosis) : Tumor necrosis factor alpha increases neuronal vulnerability to excitotoxic necrosis by inducing expression of the AMPA-glutamate receptor subunit GluR1 via an acid sphingomyelinase- and NF-kappaB dependent mechanism ... To confirm that neuronal GluR1 expression is regulated by ASMase and NF-kappaB , and to learn whether this regulation has pathophysiological significance, we treated cultured human NT2-N neurons with TNFalpha ... We conclude that TNFalpha can sensitize neurons to excitotoxic necrosis by inducing expression of GluR1 via an ASMase- and NF-kappaB dependent mechanism
Huxford et al., J Mol Biol 2002 : IkappaBalpha inhibits transcription factor NF-kappaB activity by specific binding to NF-kappaB heterodimers composed of p65 and p50 subunits
Huang et al., Cancer Res 2002 : We investigated the effects of black raspberry extracts on transactivation of activated protein 1 (AP-1) and nuclear factor kappaB (NFkappaB) induced by BaP diol-epoxide (BPDE) , the ultimate carcinogen of BaP, in mouse epidermal JB6 Cl 41 ( Cl 41 ) cells
Kaltschmidt et al., BMC molecular biology 2002 : Based on these findings, the transcriptional regulation of COX-2 by NF-kappaB was investigated ... NF-kappaB regulates neuronal COX-2 gene expression, and acts as an upstream target of Aspirin
Poulaki et al., Am J Pathol 2002 (Retinoblastoma) : We found that NF-kappaB inhibition up-regulated Bax ; down-regulated the anti-apoptotic proteins Bcl-2, A1, and cIAP-2 ; and induced loss of the mitochondrial transmembrane potential and caspase independent, calpain dependent apoptosis in Rb cells ... Inhibition of the p38 kinase sensitized cells to SN50 induced cell death, whereas insulin-like growth factor-1 activated NF-kappaB and attenuated the proapoptotic effect of SN50
Park et al., Oncogene 2002 : TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-kappaB upon gamma-radiation ... TPCK could suppress the induction of these two genes in either of Jurkat T cells and PBMCs, which might suggest the transcriptional regulation of PLCG2 and EPHX2 by NF-kappaB upon gamma-radiation
Nishi et al., Int J Mol Med 2003 (Breast Neoplasms) : However, it was found that NF-kappaB could not transactivate the EGFR by cotransfection experiments with each NF-kappaB subunit, using p50, p65 and c-Rel and an EGFR promoter luciferase reporter
Ruiz-Deya et al., J Endourol 2002 (Inflammation...) : Stimulation of the human urothelial cells by hypoxia, known to activate NF-kappa B, resulted in an increase in the levels of IL-1 and IL-6 transcripts compared with hypoxia exposed cells in the presence of NF-kappa B inhibitors ... Stimulation of the human urothelial cells by hypoxia, known to activate NF-kappa B, resulted in an increase in the levels of IL-1 and IL-6 transcripts compared with hypoxia exposed cells in the presence of NF-kappa B inhibitors
Yamamoto et al., J Immunol 2002 : A dominant negative form of TRIF inhibited TLR2-, TLR4-, and TLR7 dependent NF-kappaB activation ... Dominant negative TRIF inhibited TLR3 dependent activation of both the NF-kappaB dependent and IFN-beta promoters
Xu et al., J Immunol 2002 : TNFR associated factor-3 is associated with BAFF-R and negatively regulates BAFF-R mediated NF-kappa B activation and IL-10 production ... Moreover, overexpression of TRAF3 inhibits BAFF-R mediated NF-kappaB activation and IL-10 production ... Moreover, overexpression of TRAF3 inhibits BAFF-R mediated NF-kappaB activation and IL-10 production ... Taken together, our findings suggest that TRAF3 is a negative regulator of BAFF-R mediated NF-kappaB activation and IL-10 production
Jaramillo et al., J Immunol 2002 (MAP Kinase Signaling System) : Similarly, selective inhibition of cAMP and NF-kappaB strongly down-regulated the induction of all chemokine transcripts as well as CREB and NF-kappaB activation, respectively
Wang et al., Eur J Neurosci 2002 (Encephalitis...) : Nevertheless, silymarin could effectively reduce LPS induced superoxide generation and nuclear factor kappaB (NF-kappaB) activation
Kumar Mangalam et al., Cell Immunol 2002 : We also found that GSTM inhibited LPS induced NF-kappaB level only in ceramide responder ... We also found that GSTM inhibited LPS induced NF-kappaB level only in ceramide responder
Sheng et al., Biochem Pharmacol 2003 : While U50,488 alone had a partial inhibitory effect on constituent NF-kappaB activation, it potently suppressed Tat induced NF-kappaB activation
Littlejohn et al., Biochem Pharmacol 2003 : TNF can stimulate the proinflammatory transcription factor nuclear factor-kappaB (NF-kappaB) as well as the stress induced kinase c-Jun N-terminal kinase (JNK) through mechanisms that are not fully delineated
Philip et al., J Biol Chem 2003 : However, the molecular mechanism by which OPN activates NF kappa B and regulates pro-MMP-2 activation in murine melanoma ( B16F10 ) cells is not well defined ... We also investigated the mechanism of action of curcumin ( diferulolylmethane ) on OPN induced NF kappa B-mediated activation of pro-MMP-2 in B16F10 cells ... We also investigated the mechanism of action of curcumin ( diferulolylmethane ) on OPN induced NF kappa B-mediated activation of pro-MMP-2 in B16F10 cells ... OPN also induces the nuclear accumulation of NF kappa B p65, NF kappa B-DNA binding, and transactivation ... Moreover, our data revealed that curcumin inhibited the OPN induced translocation of p65, NF kappa B-DNA binding, and NF kappa B transcriptional activity ... To our knowledge, this is the first report that OPN induces NF kappa B activity through phosphorylation and degradation of I kappa B alpha by activating IKK that ultimately triggers the activation of pro-MMP-2 and further demonstrates that curcumin potently suppresses OPN induced cell migration, tumor growth, and NF kappa B-mediated pro-MMP-2 activation by blocking the IKK/I kappa B alpha signaling pathways ... To our knowledge, this is the first report that OPN induces NF kappa B activity through phosphorylation and degradation of I kappa B alpha by activating IKK that ultimately triggers the activation of pro-MMP-2 and further demonstrates that curcumin potently suppresses OPN induced cell migration, tumor growth, and NF kappa B-mediated pro-MMP-2 activation by blocking the IKK/I kappa B alpha signaling pathways
Bernal-Mizrachi et al., Diabetes 2002 (Insulinoma) : TNF-alpha treatment and depolarization activation of NF-kappaB differed significantly in that TNF-alpha activation was not blocked by PD98059
Budde et al., Mol Biol Cell 2002 : We have found that IkappaBbeta expression is localized within the haploid spermatid stages of spermatogenesis and follows the expression of nuclear NF-kappaB
Relić et al., Lab Invest 2002 : Of interest, TNF-alpha induced persistent nuclear factor-kappaB (NF-kappaB)-DNA binding activity even in the presence of SNP, mirroring apoptosis protection effects
Gasparian et al., Mol Cancer Ther 2002 (Prostatic Neoplasms) : Sodium selenite and MeSeA, at the concentrations that induced apoptosis, inhibited NF-kappa B DNA binding induced by tumor necrosis factor-alpha and lipopolysaccharide in DU145 and JCA1 prostate cells
Figueroa et al., Exp Hematol 2002 (Anoxia) : NF-kappaB plays a key role in hypoxia-inducible factor-1 regulated erythropoietin gene expression ... Genetic inhibition of AKT resulted in significant inhibition of NF-kappaB and HIF-1 mediated transactivation, as well as EPO gene expression, in response to hypoxia ... Overexpression of constitutively active AKT resulted in increased NF-kappaB and HIF-1 transactivation ... The selective inhibitor of NF-kappaB , pyrrolidine dithiocarbamate ( PDTC ), significantly blocked HIF-1 protein expression ... Inhibition of NF-kappaB with a superrepressor dominant negative IkappaBalpha genetic construct also significantly blocked NF-kappaB and HIF-1 transactivation, as well as EPO gene expression ... Inhibition of NF-kappaB with a superrepressor dominant negative IkappaBalpha genetic construct also significantly blocked NF-kappaB and HIF-1 transactivation, as well as EPO gene expression ... We propose a key role for NF-kappaB in EPO gene regulation in response to hypoxia
Moustafa et al., J Invest Dermatol 2002 : Inhibition of tumor necrosis factor-alpha stimulated NFkappaB/p65 in human keratinocytes by alpha-melanocyte stimulating hormone and adrenocorticotropic hormone peptides ... We have previously shown that alpha-MSH can inhibit tumor necrosis factor-alpha stimulated intercellular adhesion molecule 1 upregulation and nuclear factor kappaB (NFkappaB) transcription factor activation in melanocyte and melanoma cells ... alpha-MSH , L-Lys-L-Pro-L-Val, and L-Lys-L-Pro-D-Val all significantly inhibited tumor necrosis factor alpha stimulated NFkappaB activation, whereas ACTH 1-17 and 1-39 did not, in the HaCaT keratinocytes
Surh et al., Ann N Y Acad Sci 2002 : These ginsenosides also suppressed expression of cyclooxygenase-2 (COX-2) and activation of NF-kappaB in the TPA treated dorsal skin of mice
Jin et al., Ann N Y Acad Sci 2002 (Colonic Neoplasms) : Thalidomide inhibited interleukin (IL) 1beta induced NFkappaB transcriptional activation and IL-8 production in Caco-2 colon cancer cells
Seo et al., Ann N Y Acad Sci 2002 (Adenocarcinoma...) : NF-kappaB might play an important role in COX-2 expression in H. pylori induced gastric inflammation as compared to other transcription factors
Tzima et al., EMBO J 2002 : Additionally, Rac1 mediates flow induced stimulation of nuclear factor kappaB (NF-kappaB) and the subsequent expression of intercellular cell adhesion molecule 1 ( ICAM-1 ), an adhesion receptor involved in the recruitment of leukocytes to atherosclerotic plaque
Stehlik et al., J Exp Med 2002 : While coexpression of ASC with certain PAAD-family proteins such as Pyrin and Cryopyrin increases NF-kappaB activity, ASC has an inhibitory influence on NF-kappaB activation by various proinflammatory stimuli, including tumor necrosis factor (TNF)alpha, interleukin 1beta, and lipopolysaccharide (LPS)
Klein et al., Genes Immun 2002 (Colitis, Ulcerative) : Interleukin 11 (IL11) mediates anti-inflammatory effects and is able to downregulate LPS induced NF(kappa)B activation
Gukovsky et al., Am J Physiol Gastrointest Liver Physiol 2003 (Pancreatitis, Alcoholic) : Curcumin also blocked CCK induced NF-kappaB and AP-1 activation in isolated pancreatic acini
Jung et al., J Biol Chem 2003 : The ability of MDAs to induce HIF-1alpha is dependent upon activation of NFkappaB , since inhibition of NFkappaB either pharmacologically or by transfection of an NFkappaB super-repressor plasmid abrogated this induction
Ikeda et al., J Leukoc Biol 2002 : Furthermore, IL-10 suppressed lipopolysaccharide (LPS) induced NF-kappaB binding but not Sp-1 ... Furthermore, IL-10 suppressed lipopolysaccharide (LPS) induced NF-kappaB binding but not Sp-1 ... The combination of activated Sp-1 and STATs is important for IL-10 induced MCP-1 expression in resting monocytes/macrophages, and the inhibition of LPS induced NF-kappaB binding is crucial for down-regulation of MCP-1 by IL-10 in stimulated monocytes/macrophages
Cui et al., Zhonghua Jie He He Hu Xi Za Zhi 2002 (Asthma...) : NF-kappa B and AP-1 may play important roles in the regulation of VCAM-1 and eotaxin
Masaki et al., Eur J Biochem 2003 : Mixed lineage kinase LZK and antioxidant protein-1 activate NF-kappaB synergistically
Minami et al., J Biol Chem 2003 : Taken together, these data suggest that the endothelial cell-specific effect of thrombin on VCAM-1 expression involves the coordinate activity of PKC-delta-NF-kappaB and PKC-zeta-GATA signaling pathways
Varney et al., In Vivo 2002 (Melanoma...) : The differential expression of IL-8 by monocytes following MCP-1 and M-CSF treatments involved activation of the NFkB transcription factor
Medina et al., Microb Pathog 2002 : The objective of this study was to investigate the ability of group A streptococci (GAS) to stimulate the nuclear translocation of NF-kappaB in cultured human epithelial ( HEp-2 ) cells
Komarova et al., J Biol Chem 2003 : RANK ligand (RANKL) induces activation of NFkappaB , enhancing the formation, resorptive activity, and survival of osteoclasts ... RANKL induced transient translocation of NFkappaB to the nuclei, which was maximal at 15 min. or BAPTA delayed nuclear translocation of NFkappaB
Dienz et al., J Immunol 2003 : In contrast, replenishment of SLP-76 or PLCgamma1 expression restored CD3/CD28 induced IkappaB kinase (IKK) activity as well as NF-kappaB DNA binding and transactivation ... In contrast, Vav1 induced NF-kappaB activation was normal in SLP-76 ( - ) cells, but absent in PLCgamma1 ( - ) cells
Häcker et al., Cancer Cell 2002 (Cell Transformation, Neoplastic...) : Is NF-kappaB2/p100 a direct activator of programmed cell death ?
Dhawan et al., Cancer Res 2002 (Disease Progression...) : The purpose of this study was to explore the role of Akt in the activation of NF-kappaB during melanoma tumor progression
Mandrekar et al., Alcohol Clin Exp Res 2002 (Translocation, Genetic) : Acute alcohol treatment blocks inflammatory cytokines via inhibition of NF-kappaB in monocytes in the presence of ongoing IkappaBalpha degradation, suggesting regulation of NF-kappaB activation downstream of IkappaBalpha degradation
Azevedo-Martins et al., Diabetes 2003 (Insulinoma...) : In contrast, the suppression of MnSOD gene expression in cytokine stimulated MnSOD antisense cells resulted in a threefold higher activation of NF-kappaB and a twofold higher activation of the iNOS promoter as compared with control cells
Fan et al., Arch Biochem Biophys 2003 : The MGO-BSA induced NFkappaB activation was prevented in the presence of PD98059, NAC , and parthenolide, a selective inhibitor of NFkappaB
Németh et al., Immunol Lett 2003 : In the present paper we investigated the effect of pharmacological inhibition of NF-kappaB with pyrrolidinedithiocarbamate ( PDTC ) on IL-1beta induced IL-8 production by the human intestinal epithelial cell line HT-29
Salleh et al., Toxicology 2003 : DES induced the expression of 12 genes ( bad, bax, bcl-x, caspase-1, p53, cyclin D3, GADD45, p21, p15, p27, p57 and Skp1 ) and down-regulated the expression of eight genes ( bcl-2, caspase-2, caspase-7, caspase-8, E124, iNOS, mdm2 and NFkappab1 ) at twofold or greater levels
Storz et al., EMBO J 2003 : Protein kinase D mediates a stress induced NF-kappaB activation and survival pathway ... PKD activates NF-kappaB through the IKK complex and more specifically, IKKbeta, leading to I(kappa)B(alpha) degradation
Yang et al., Chin J Cancer 2002 (Liver Neoplasms) : 2. TPCK could inhibit the activation of NF-kappa B induced by TNF-alpha
Pollet et al., Immunopharmacol Immunotoxicol 2002 : Our results showed that diHDA-glycerol induced a rapid NF-kappaB translocation as a consequence of IkappaB-alpha proteolysis
Rahman et al., Blood 2003 (MAP Kinase Signaling System) : The treatment of RAW264 cells with TSA and NaB inhibited TNF-alpha induced nuclear translocation of NF-kappa B and sRANKL induced activation of p38 mitogen activated protein kinase ( MAPK ) signals ... The treatment of RAW264 cells with TSA and NaB inhibited TNF-alpha induced nuclear translocation of NF-kappa B and sRANKL induced activation of p38 mitogen activated protein kinase ( MAPK ) signals
Dopp et al., Neurochem Res 2002 : Expression of the p75 TNF receptor is linked to TNF induced NFkappaB translocation and oxyradical neutralization in glial cells
Qian et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2002 (Hematologic Neoplasms) : However, TRAIL can also trigger transcriptional activations of the pro-oncogene of c-fos, JNK, and NF-kappaB by other signaling pathways downstream of FADD/caspase-8
Nickols et al., Nat Neurosci 2003 : Inhibiting NF-kappaB also prevented activation of Oct-6 , a transcription factor induced by axonal contact and required for proper myelin formation
Weber et al., Am J Physiol Endocrinol Metab 2003 : In previous studies, we have correlated the inhibitory actions of PGJ ( 2 ) on inducible nitric oxide synthase (iNOS) expression and NF-kappaB activation in response to IL-1 with the increased expression of HSP70 ... Using overexpression and antisense depletion, we provide evidence that HSP70 does not mediate the inhibitory actions of PGJ ( 2 ) on IL-1 induced NF-kappaB or IFN-gamma induced STAT1 activation or cytokine stimulated iNOS expression by beta-cells
Ashton et al., J Biol Chem 2003 : Inhibition of tumor necrosis factor alpha mediated NFkappaB activation and leukocyte adhesion, with enhanced endothelial apoptosis, by G protein linked receptor ( TP ) ligands ... IBOP abrogated TNFalpha induced NFkappaB activation in endothelial cells, as determined by reduced IkappaB phosphorylation and NFkappaB nuclear translocation, by inhibiting the assembly of signaling intermediates with the intracellular domain of TNF receptors 1 and 2 in response to TNFalpha
Wang et al., J Immunol 2003 : Furthermore, LPS , when combined with sCD14, resulted in NF-kappaB activation in both SW480 and SW620 cells ... These results provide direct evidence that although SW480 and SW620 cells do not express membrane bound CD14, LPS in the presence of sCD14 can activate NF-kappaB , up-regulate beta ( 1 ) integrin expression, and subsequently promote tumor cell adhesion and invasion
Maruyama et al., J Immunol 2003 : The induction of the IL-12 p40 gene is regulated by NF-kappaB in the presence of IFN-gamma ... The induction of the IL-12 p40 gene is regulated by NF-kappaB in the presence of IFN-gamma ... The induction of the IL-12 p40 gene is regulated by NF-kappaB in the presence of IFN-gamma
Renshaw et al., J Immunol 2003 : No additional proapoptotic effect of leucine zipper TRAIL was identified following TRAIL treatment of neutrophils in the presence of gliotoxin, an inhibitor of NF-kappaB, suggesting TRAIL does not activate NF-kappaB in human neutrophils
Steer et al., J Immunol 2003 : In contrast, inhibition of NF-kappaB activation prevents dsRNA stimulated COX-2 expression and PGE ( 2 ) accumulation by macrophages
Kawakita et al., Biol Pharm Bull 2003 : The effect of inhibitors of activator protein-1 (AP-1) and nuclear factor kappaB (NF-kappaB) on the radiation induced gamma-GCS gene expression was then examined
Bour-Jordan et al., Nat Immunol 2003 : CTLA-4 deficiency also affects the balance of the nuclear factors NFATc1 and NFATc2, and enhances activation of NF-kappaB
Byun et al., Exp Mol Med 2002 : In contrast, exposure to oxidative stress in certain cell types induced reduction of tumor necrosis factor (TNF)- induced NF-kappaB activation ... And various thiol modifying agents including gold compounds and cyclopentenone prostaglandins inhibit NF-kappaB activation by blocking IkappaB kinase (IKK) ... To understand such conflicting effect of oxidative stress on NF- kappakB activation, HeLa cells were incubated with H ( 2 ) O ( 2 ) or diamide and TNF induced expression of NF-kappaB reporter gene was measured
Sancho et al., Mol Pharmacol 2003 : This study shows that AEA inhibits tumor necrosis factor-alpha (TNFalpha) induced NF-kappaB activation by direct inhibition of the IkappaB kinase (IKK)beta and, to a lesser extent, the IKKalpha subunits of kappaB inhibitor ( IkappaB) kinase complex, and that IKKs inhibition by AEA correlates with inhibition of IkappaBalpha degradation, NF-kappaB binding to DNA, and NF-kappaB dependent transcription in TNFalpha stimulated cells ... The NF-kappaB inhibitory activity of AEA was independent of CB(1) and CB(2) activation in TNFalpha stimulated 5.1 and A549 cell lines, which do not express vanilloid receptor 1, and was not mediated by hydrolytic products formed through the activity of the enzyme fatty acid amide hydrolase ... The NF-kappaB inhibitory activity of AEA was independent of CB(1) and CB(2) activation in TNFalpha stimulated 5.1 and A549 cell lines, which do not express vanilloid receptor 1, and was not mediated by hydrolytic products formed through the activity of the enzyme fatty acid amide hydrolase
Bentires-Alj et al., Oncogene 2003 (Colonic Neoplasms) : Indeed, the inhibition of NF-kappaB reduced mdr1 mRNA and P-glycoprotein expression in HCT15 cells
Lacey et al., Arthritis Rheum 2003 (Hyperplasia) : Activation of NF-kappa B was induced by IL-1 beta , but not by MIF ... Activation of NF-kappa B was induced by IL-1 beta, but not by MIF ... Anti-MIF mAb had no effect on IL-1 beta induced NF-kappa B nuclear translocation
Bayon et al., Mol Cell Biol 2003 : MX781 was able to completely inhibit tumor necrosis factor alpha mediated activation of IkappaB kinase (IKK) , the upstream regulator of NF-kappaB
Xie et al., Blood 2003 : During cell culture, beta ( 2 ) M down-regulated the expression of phosphorylated mitogen activated protein ( MAP ) kinases , extracellular signal related kinase ( ERK ), and mitogen induced extracellular kinase ( MEK ), inhibited nuclear factor-kappaB (NF-kappaB) , and activated signal transducer and activator of transcription-3 ( STAT3 ) in treated cells, all of which are involved in cell differentiation and proliferation ... Thus, our study demonstrates that beta ( 2 ) M at high concentrations retards the generation of MoDCs, which may involve down-regulation of major histocompatibility complex class I molecules, inactivation of Raf/MEK/ERK cascade and NF-kappaB , and activation of STAT3 , and it merits further study to elucidate the underlying mechanisms
Raschi et al., Blood 2003 (Antiphospholipid Syndrome...) : Delta TRAF6 and Delta MyD88 significantly abrogate antibody induced as well as IL-1- or LPS induced NF-kappa B activation, whereas Delta TRAF2 ( involved in NF-kappa B activation by TNF ) does not affect it ... Delta TRAF6 and Delta MyD88 significantly abrogate antibody induced as well as IL-1- or LPS induced NF-kappa B activation , whereas Delta TRAF2 ( involved in NF-kappa B activation by TNF ) does not affect it
Lindgren et al., Biochem Biophys Res Commun 2003 : Taken together, our data implicate distinct roles for IkappaBalpha and IkappaBbeta in regulating various NF-kappaB activities ... Taken together, our data implicate distinct roles for IkappaBalpha and IkappaBbeta in regulating various NF-kappaB activities
Fujioka et al., Clin Cancer Res 2003 (Neoplasm Metastasis...) : Inhibiting NFkappaB activation by expressing IkappaBalphaM and using pharmacologic NFkappaB inhibitor PS-341 also significantly reduced cytokine induced vascular endothelial growth factor and interleukin-8 expression in AsPc-1 pancreatic cancer cells ... Inhibiting NFkappaB activation by expressing IkappaBalphaM and using pharmacologic NFkappaB inhibitor PS-341 also significantly reduced cytokine induced vascular endothelial growth factor and interleukin-8 expression in AsPc-1 pancreatic cancer cells
Burns et al., J Exp Med 2003 : In contrast, expression of MyD88 short (MyD88s), an alternatively spliced form of MyD88 that lacks only the short intermediate domain separating the DD and TIR domains, leads to a shutdown of IL-1/lipopolysaccharide induced NF-kappaB activation ... In the presence of MyD88s , IRAK-1 is not phosphorylated and neither activates NF-kappaB nor is ubiquitinated
Kiriakidis et al., J Cell Sci 2003 (Inflammation...) : VEGF expression in human macrophages is NF-kappaB dependent : studies using adenoviruses expressing the endogenous NF-kappaB inhibitor IkappaBalpha and a kinase-defective form of the IkappaB kinase 2 ... Because LPS and CD40L activate nuclear factor-kappaB (NF-kappaB) in monocytes, we investigated in this study whether VEGF production in macrophages, when stimulated with either LPS or CD40L, is NF-kappaB dependent ... Because LPS and CD40L activate nuclear factor-kappaB (NF-kappaB) in monocytes, we investigated in this study whether VEGF production in macrophages, when stimulated with either LPS or CD40L, is NF-kappaB dependent ... Because LPS and CD40L activate nuclear factor-kappaB (NF-kappaB) in monocytes, we investigated in this study whether VEGF production in macrophages, when stimulated with either LPS or CD40L, is NF-kappaB dependent ... These results show for the first time that VEGF production in human macrophages is NF-kappaB dependent
Brueckmann et al., Thromb Haemost 2003 : Electrophoretic mobility shift assays revealed that APC attenuated NF-kappaB DNA binding capacity implying that NF-kappaB may not be involved in the upregulatory effect of APC on MCP-1 production
Park et al., Cytokine 2002 : Together, the p43 induced TNF production was controlled by NFkB , p38 MAPK, and ERK that is dependent on the activities of PLC and PKC
Biswas et al., Cancer Res 2003 (Adenocarcinoma...) : Both the protein kinase C (alpha/beta) inhibitor Go6976 and expression of dominant negative nuclear factor (NF)-kappaB inhibitor kinase mutants : ( a ) blocked the growth and caused regression of a mammary tumor insyngeneic mice ; ( b ) inhibited epidermal growth factor (EGF) induced activation, nuclear translocation, and DNA binding activity of NF-kappaB ; and ( c ) caused apoptosis of EGF stimulated cultured mammary tumor cells
Arsura et al., Oncogene 2003 (Carcinoma, Hepatocellular...) : Transient activation of NF-kappaB through a TAK1/IKK kinase pathway by TGF-beta1 inhibits AP-1/SMAD signaling and apoptosis : implications in liver tumor formation ... Transient activation of NF-kappaB through a TAK1/IKK kinase pathway by TGF-beta1 inhibits AP-1/SMAD signaling and apoptosis : implications in liver tumor formation ... Transient activation of NF-kappaB through a TAK1/IKK kinase pathway by TGF-beta1 inhibits AP-1/SMAD signaling and apoptosis : implications in liver tumor formation ... Here we elucidate the mechanism of TGF-beta1 mediated regulation of NF-kappaB and induction of apoptosis in epithelial cells ... Inhibition of NF-kappaB following TGF-beta1 treatment increased AP-1 complex transcriptional activity through sustained c-Jun phosphorylation, thereby potentiating AP-1/SMADs mediated cell killing ... Inhibition of NF-kappaB following TGF-beta1 treatment increased AP-1 complex transcriptional activity through sustained c-Jun phosphorylation, thereby potentiating AP-1/SMADs mediated cell killing ... Furthermore, TGF-beta1 mediated upregulation of Smad7 appeared independent of NF-kappaB
Takaesu et al., J Mol Biol 2003 : In contrast to previous results with mouse embryo fibroblasts lacking either IKKalpha or IKKbeta, which indicated that only IKKbeta is involved in cytokine induced NF-kappaB activation, we found that both IKKalpha and IKKbeta were important in activating the NF-kappaB pathway
Engedal et al., J Biol Chem 2003 : Second, we observed an excellent correlation between repression of TPA induced NF kappa B activation by an irreversible inhibitor of I kappa B alpha phosphorylation, BAY11-7082, and its ability to abrogate TPA induced suppression of Fas mediated apoptosis
Watanabe et al., Anticancer Res 2002 (Carcinoma, Squamous Cell...) : The induction of inducible nitric oxide synthase is regulated by nuclear-factor kappa B (NF-kappa B)
Hofmann et al., Biol Chem 2002 : While the biochemical mechanisms mediating repression of NF-kappaB activity by glucocorticoids (GCs) are relatively well studied, the role of promoter architecture for the effects of GCs on NF-kappaB remains poorly characterized
Millán et al., Oncogene 2003 : H-Ras-specific activation of NF-kappaB protects NIH 3T3 cells against stimulus dependent apoptosis ... In this study, the basal and tumor necrosis factor alpha (TNFalpha) induced activation of NF-kappaB has been examined in cells overexpressing H-Ras, K-Ras or N-Ras
Sanjo et al., Mol Cell Biol 2003 (Fetal Death...) : Recently, serine/threonine kinase TAK1 and TAK1 binding protein 1 and 2 (TAB1/2) have been identified as molecules involved in IL-1 induced TRAF6 mediated activation of AP-1 and NF-kappa B via mitogen activated protein ( MAP ) kinases and I kappa B kinases, respectively ... Recently, serine/threonine kinase TAK1 and TAK1 binding protein 1 and 2 (TAB1/2) have been identified as molecules involved in IL-1 induced TRAF6 mediated activation of AP-1 and NF-kappa B via mitogen activated protein ( MAP ) kinases and I kappa B kinases, respectively ... However, the IL-1 induced activation of NF-kappa B and MAP kinases was not impaired in TAB2-deficient embryonic fibroblasts
Müller et al., J Biol Chem 2003 : Lymphotoxin beta receptor induces sequential activation of distinct NF-kappa B factors via separate signaling pathways ... Lymphotoxin beta receptor (LTbetaR) induced activation of NF-kappaB in mouse embryo fibroblasts was mediated by the classical pathway and by an alternative or second pathway ... The sequential engagement of two distinct pathways, coupled with the negative feedback inhibition of RelA complexes by NF-kappaB induced resynthesis of IkappaBalpha , resulted in a pronounced temporal change in the nature of the NF-kappaB activity during the course of stimulation
De Ridder et al., Br J Cancer 2003 : Both iNOS activation and radioresponse were impaired by the NF-kappaB inhibitors phenylarsine oxide and lactacystin
Dalen et al., Br J Cancer 2003 (Lymphoma) : Activation of NF-kappaB by TNF-alpha prior to TRAIL exposure increased resistance of the cells to TRAIL mediated apoptosis
Ajmone-Cat et al., J Neurochem 2003 : In LPS activated microglia, PS-liposomes did not affect NF-kappaB activation but inhibited the phosphorylation of p38 and delayed that of CREB
Nasr et al., Blood 2003 (Leukemia-Lymphoma, Adult T-Cell) : The As/IFN combination reduced Tax expression and, accordingly, reversed the Tax induced constitutive nuclear factor kappaB (NF-kappaB) activation
Curry et al., Arch Pathol Lab Med 2003 (Psoriasis) : Exposure of fibroblasts, but not KCs , to lipopolysaccharide or HSPs triggered nuclear factor (NF)-kappaB activation
Saijo et al., Nat Immunol 2003 : Impaired NF-kappaB induction was overcome by the activation of protein kinase C ( PKC ) -lambda, thus suggesting the involvement of PKC-lambda in pre-BCR mediated SFK dependent activation of NF-kappaB ... Our data show the existence of a functionally distinct SFK signaling module responsible for pre-BCR mediated NF-kappaB activation and B cell development
Eto et al., Biochem Biophys Res Commun 2003 : Essential roles for NF-kappa B and a Toll/IL-1 receptor domain-specific signal ( s ) in the induction of I kappa B-zeta
Jiang et al., Biochem Biophys Res Commun 2003 : By using luciferase assay, we found that both IKK alpha and IKK beta could activate NF-kappa B in synergy with NIK ... By using luciferase assay, we found that both IKK alpha and IKK beta could activate NF-kappa B in synergy with NIK
Santos et al., J Biol Chem 2003 (Skin Neoplasms) : Given the roles of Akt in NF-kappa B signaling and the importance of these processes in the epidermis, a study was made into the possible alterations of the NF-kappa B pathway in transgenic mice expressing K10 in the proliferative basal layer ... It was found that the inhibition of Akt , mediated by K10 expression, leads to impaired NF-kappa B activity ... It was found that the inhibition of Akt, mediated by K10 expression, leads to impaired NF-kappa B activity
Higuchi et al., Antioxid Redox Signal 2002 (Leukemia, Myeloid, Acute) : Additionally, tumor necrosis factor (TNF) induced activation of NF-kappaB and AP-1 observed in ML-1a was greatly reduced in clone 19
Sakamoto et al., J Clin Endocrinol Metab 2003 (Endometriosis) : We hypothesize that TNFalpha may induce IL-8 production in endometriotic cells through nuclear factor-kappa B (NF-kappa B) activation ... We hypothesize that TNFalpha may induce IL-8 production in endometriotic cells through nuclear factor-kappa B (NF-kappa B) activation ... These findings demonstrate that NF-kappa B activation is critical for TNFalpha induced IL-8 expression in endometriotic stromal cells ... The current study showed for the first time that GnRHa treatment attenuated the expression of IL-8 by reducing TNFalpha induced NF-kappa B activation
Sen et al., J Immunol 2003 (Diabetes Mellitus, Type 1) : Furthermore, increased NF-kappaB activation resulted in an enhanced capacity of NOD vs NOR or BALB/c Mphi to secrete IL-12 ( p70 ), TNF-alpha, and IL-1alpha, which was inhibited upon infection with an adenoviral recombinant encoding a modified form of IkappaBalpha
Johnson et al., J Immunol 2003 : Cotransfecting NF-kappaB1 with RelA further increased activity of the HLA-A2 and -B7, but not HLA-Cw6, promoters ... Cotransfecting NF-kappaB1 with RelA further increased activity of the HLA-A2 and -B7 , but not HLA-Cw6, promoters
Artis et al., J Immunol 2003 (Leishmaniasis, Cutaneous) : Critically, the frequency of proliferating KO CD4 ( + ) T cells secreting IFN-gamma matched that of wild-type cells, suggesting that NF-kappaB1 was not required for efficient transcription of the IFN-gamma gene ... Taken together, these results identify a novel role for NF-kappaB1 in CD4 ( + ) T cell proliferation and the development of Th1 cell responses required for protective immunity against intracellular pathogens
Hauf et al., J Immunol 2003 (Inflammation) : We found that STEC, enterohemorrhagic E. coli, and enteropathogenic E. coli all interfere with NF-kappaB activation initiated by TNF-alpha , indicating that suppression of signal induced NF-kappaB activity is a property common to several attaching and effacing bacteria ... As a consequence of NF-kappaB suppression, wild-type STEC induces significantly lower mRNA levels of IL-8, IL-6, and IL-1alpha upon prolonged infection periods compared with bacteria lacking EspB ... As a consequence of NF-kappaB suppression, wild-type STEC induces significantly lower mRNA levels of IL-8, IL-6 , and IL-1alpha upon prolonged infection periods compared with bacteria lacking EspB ... As a consequence of NF-kappaB suppression, wild-type STEC induces significantly lower mRNA levels of IL-8 , IL-6, and IL-1alpha upon prolonged infection periods compared with bacteria lacking EspB
Mendes et al., J Cell Biochem 2003 : Differential roles of hydrogen peroxide and superoxide in mediating IL-1 induced NF-kappa B activation and iNOS expression in bovine articular chondrocytes ... Taken together, the results obtained indicate that superoxide mediates IL-1 induced I kappa B-alpha degradation and the consequent NF-kappa B activation and iNOS expression in chondrocytes, whereas H ( 2 ) O ( 2 ) does not seem to participate in those IL-1 induced responses
Carlson et al., Am J Physiol Heart Circ Physiol 2003 (Burns...) : IkappaBalpha overexpression or ALLN treatment of burn trauma prevented NF-kappaB activation in cardiac tissue, prevented cardiomyocyte secretion of TNF-alpha, and ablated burn mediated cardiac contractile dysfunction
Ahn et al., Clin Orthop Relat Res 2003 (Myositis Ossificans) : To clarify the potential role of NF-kappaB in the pathophysiologic features of fibrodysplasia ossificans progressiva, the role of NF-kappaB in regulating bone morphogenetic protein-4 signaling in patient derived lymphoblastoid cell lines was examined
Ip et al., Clin Exp Allergy 2003 (Hypereosinophilic Syndrome) : In vitro studies of EoL-1 cells, an eosinophilic leukaemic cell line, confirmed that NF-kappaB plays an important role in the expression of ICAM-1 and recruitment of eosinophils in allergic inflammation
Ibuki et al., Biochim Biophys Acta 2003 : We concluded that ( 1 ) the increase in NO production was due to direct DNA damage by gamma-irradiation, and that ( 2 ) PARP activation through DNA damage induced NF-kappaB activation, leading to iNOS expression and NO production
Li et al., Yao Xue Xue Bao 2001 : Hydrocortisone inhibited TNF-alpha stimulated PMN-HSC adhesion, and expression of VCAM-1 by suppressing the activity of NF-kappa B
Bengoechea-Alonso et al., Nitric Oxide 2003 : These observations are consistent with the inhibition caused by W-7, NMLA, H-89, and cyclosporin A on NF-kappaB induction by these agonists, indicating the involvement of PKA and PP2B in the regulation of NF-kappaB in human monocytes ... These observations are consistent with the inhibition caused by W-7, NMLA, H-89, and cyclosporin A on NF-kappaB induction by these agonists, indicating the involvement of PKA and PP2B in the regulation of NF-kappaB in human monocytes
Hirano et al., Int Immunopharmacol 2003 : Moreover, bezafibrate repressed TNF-alpha induced DNA binding activity of NF-kappaB ... Thus, fibrates reduced TNF-alpha induced NF-kappaB activation and RANTES expression, possibly suggesting that fibrates might be inhibitory agents of migration of inflammatory cells by RANTES to the liver in patients with alcoholic liver diseases
Witcher et al., Blood 2003 (Acute Disease...) : Underlying this response was an increase in TNF induced nuclear factor-kappaB (NF-kappaB) DNA binding within 2 hours in the presence of ATRA and activation of NF-kappaB DNA binding and transcriptional activity in response to ATRA alone within 48 hours of ATRA treatment
Parnet et al., J Neuroendocrinol 2003 : Using immunohistochemistry with an antibody directed against the p65 NF kappa B subunit, a rapid and transient NF kappa B response to LPS was observed ... Activation of NF kappa B in response to IL-1 beta was no longer apparent in IL-1RI knockout mice, confirming that this receptor is essential for the transduction of IL-1 signal in the pituitary, but remained after LPS treatment
Tang et al., J Clin Invest 2003 : NF-kappaB activation and IL-8 secretion were attenuated by the NF-kappaB inhibitors pyrrolidine dithiocarbamate and cell-permeable peptide
Espinosa et al., Mol Biol Cell 2003 : We have previously described that expression of a p65-NFkappaB mutant that lacks the transactivation domain ( p65DeltaTA ) induces cytoplasmic translocation of N-CoR leading to a positive regulation of different promoters
Hammes et al., Nat Med 2003 (Diabetes Mellitus, Experimental...) : We have discovered that the lipid-soluble thiamine derivative benfotiamine can inhibit these three pathways, as well as hyperglycemia associated NF-kappaB activation, by activating the pentose phosphate pathway enzyme transketolase , which converts glyceraldehyde-3-phosphate and fructose-6-phosphate into pentose-5-phosphates and other sugars ... In retinas of diabetic animals, benfotiamine treatment inhibited these three pathways and NF-kappaB activation by activating transketolase , and also prevented experimental diabetic retinopathy
Lee et al., J Immunol 2003 : IL-1 beta increased NF-kappa B and AP-1 activity and both transcription factors mediated IL-1 beta induced MCP-1 expression in mesangial cells ... In contrast, IL-1 beta induced NF-kappa B activity and I kappa B alpha degradation were not affected by BMP-7 ... In contrast, IL-1 beta induced NF-kappa B activity and I kappa B alpha degradation were not affected by BMP-7 ... In contrast, IL-1 beta induced NF-kappa B activity and I kappa B alpha degradation were not affected by BMP-7
Ishii et al., Biochim Biophys Acta 2003 : Gel shift analysis revealed that stimulation of macrophages with LPS caused an increase in the DNA binding activity of nuclear factor-kappaB (NF-kappaB) , which was inhibited by isomallotochromanol and isomallotochromene
Schilling et al., Infect Immun 2003 : The LPS receptor complex utilized by the bladder epithelial cell lines included CD14 and Toll-like receptors, and signaling involved the activation of NF-kappaB and p38 mitogen activated protein kinase ... The LPS receptor complex utilized by the bladder epithelial cell lines included CD14 and Toll-like receptors, and signaling involved the activation of NF-kappaB and p38 mitogen activated protein kinase
Suzawa et al., Nat Cell Biol 2003 : Unlike suppression of the PPAR-gamma transactivation function by mitogen activated protein kinase induced growth factor signalling through phosphorylation of the A/B domain, NF-kappaB blocks PPAR-gamma binding to DNA by forming a complex with PPAR-gamma and its AF-1-specific co-activator PGC-2
Wong et al., Am J Respir Cell Mol Biol 2003 : The phosphorylation of inhibitor kappaB-alpha and p38 mitogen activated protein kinase ( MAPK ) was detected by Western blot, whereas NF-kappaB activity was measured by electrophoretic mobility shift assay ... IL-3, IL-5, and GM-CSF could enhance p38 MAPK and NF-kappaB activity and induce ICAM-1 , CD11b, and CD18 expressions on eosinophils ... IL-3, IL-5, and GM-CSF could enhance p38 MAPK and NF-kappaB activity and induce ICAM-1, CD11b , and CD18 expressions on eosinophils ... IL-3, IL-5, and GM-CSF could enhance p38 MAPK and NF-kappaB activity and induce ICAM-1, CD11b, and CD18 expressions on eosinophils ... IL-3, IL-5, and GM-CSF could enhance p38 MAPK and NF-kappaB activity and induce ICAM-1, CD11b, and CD18 expressions on eosinophils ... IL-3 , IL-5, and GM-CSF could enhance p38 MAPK and NF-kappaB activity and induce ICAM-1, CD11b, and CD18 expressions on eosinophils ... IL-3, IL-5 , and GM-CSF could enhance p38 MAPK and NF-kappaB activity and induce ICAM-1, CD11b, and CD18 expressions on eosinophils ... Therefore, the expression of ICAM-1 might involve both p38 MAPK and NF-kappaB activities, whereas the regulation of CD11b, CD18, and ICAM-3 expressions might be mediated through p38 MAPK but not NF-kappaB
Takeshita et al., Am J Respir Cell Mol Biol 2003 (Acidosis, Respiratory...) : Exposure to HA significantly attenuated LPS induced NF-kappaB activation through suppressing IkappaB-alpha degradation
Yang et al., Invest Ophthalmol Vis Sci 2003 : This technique will be useful to determine the role of NF-kappaB in experimental proliferative vitreoretinopathy (PVR) , and may offer a novel approach to treatment of PVR with a gene therapy approach
Imanishi et al., Clin Exp Pharmacol Physiol 2003 : Inhibition of NF-kappaB activation by SN50 suppressed c-IAP1 protein expression and enhanced apoptosis induced by either TNF-alpha or CH11
Suzuki et al., Immunology 2003 : These inhibitors interfered with CD86 gene transcription in the presence of activated NF-kappaB , whereas phosphorylated CREB was down-regulated in the reactions where these inhibitors were added to inhibit CD86 gene expression
Guo et al., J Neurochem 2003 : Caffeic acid phenethyl ester, a potent and specific inhibitor of activation of NF-kappaB, not only blocked IL-1beta induced activation of the NF-kappaB promoter but also decreased IL-1beta induced MIP-1alpha and -1beta expression in NT2-N cells ... These data suggest that NF-kappaB is at least partially involved in the IL-1beta mediated action on MIP-1alpha and -1beta in NT2-N cells ... These data suggest that NF-kappaB is at least partially involved in the IL-1beta mediated action on MIP-1alpha and -1beta in NT2-N cells
Ramana et al., Chem Biol Interact 2003 : Stimulation with TNF-alpha led to the activation of the transcription factor NF-kappaB and enhanced VSMC growth
Delgado et al., Biochem Biophys Res Commun 2003 (Inflammation) : VIP seems to act by inhibiting the NF-kappaB dependent IL-8 gene activation
Agresti et al., Biochem Biophys Res Commun 2003 : Functionally, HMGB1 is required for the NF-kappaB dependent expression of the adhesion molecule VCAM-1
Schiekofer et al., Diabetes 2003 (Hyperglycemia) : Inhibition of activation of ras, MAPK , or protein kinase C blocked hyperglycemia mediated NF-kappaB activation in ex vivo isolated PBMCs stimulated with 10 mmol/l glucose
Kang et al., J Biol Chem 2003 : The NF-kappaB mediated iNOS induction was stimulated by the overexpression of activated mutants of Galpha(12/13) ( Galpha(12/13)QL ) ... Protein kinase C depletion inhibited I-kappaBalpha degradation, NF-kappaB activation, and iNOS induction by thrombin or the iNOS induction by Galpha(12/13)QL ... JNK inhibition by the stable transfection with a dominant negative mutant of JNK1 ( JNK1 ( - ) ) completely suppressed the NF-kappaB mediated iNOS induction by thrombin ... In addition, JNK and p38 kinase oppositely controlled the NF-kappaB mediated iNOS induction by Galpha(12/13)QL
Suk et al., J Pharmacol Exp Ther 2003 : Moreover, baicalein inhibited LPS induced nuclear factor-kappaB (NF-kappaB) activity in BV-2 cells without affecting caspase-11 activation, interferon regulatory factor-1 induction, or signal transducer and activator of transcription-1 phosphorylation
Chen et al., J Pharmacol Exp Ther 2003 : Ad.N17Rac1 did not inhibit TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) binding activity or inhibitor of NF-kappaB-alpha degradation ... Ad.N17Rac1 did not inhibit TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) binding activity or inhibitor of NF-kappaB-alpha degradation
Majumdar et al., Oncogene 2003 (Leukemia, Myeloid) : The treatment of these cells with adenosine suppressed TNF induced NF-kappaB activation, but had no effect on activation of another redox-sensitive transcription factor, AP-1 ... The effect on TNF induced NF-kappaB activation was selective as adenosine had minimal effect on NF-kappaB activation induced by H ( 2 ) O ( 2 ), PMA, LPS, okadaic acid, or ceramide, suggesting differences in the pathway leading to NF-kappaB activation by different agents ... The effect on TNF induced NF-kappaB activation was selective as adenosine had minimal effect on NF-kappaB activation induced by H ( 2 ) O ( 2 ), PMA, LPS , okadaic acid, or ceramide, suggesting differences in the pathway leading to NF-kappaB activation by different agents ... The suppression of TNF induced NF-kappaB activation by adenosine was found not to be because of inhibition of TNF induced IkappaBalpha phosphorylation and degradation or IkappaBalpha kinase activation ... The suppression of TNF induced NF-kappaB activation was unique to adenosine, as neither its metabolites ( inosine, AMP, and ATP ) nor pyrimidines ( thymidine and uridine ) had any effect ... Overall, our results clearly demonstrate that adenosine selectively suppresses TNF induced NF-kappaB activation, which may contribute to its role in suppression of inflammation and of the immune system
Kuo et al., J Biomed Sci 2003 : These results suggest that LTA might activate PC-PLC and PI-PLC to induce PKC activation, which in turn initiates NF-kappaB activation, and finally induces iNOS expression and NO release in RAW 264.7 macrophages
Jiang et al., J Biol Chem 2003 (MAP Kinase Signaling System) : However, we find that, in response to dsRNA, TLR3 can mediate the activation of both NFkappaB and mitogen activated protein ( MAP ) kinases in IL-1-unresponsive mutant cell lines, including IRAK-deficient I1A and I3A cells, which are defective in a component that is downstream of IL-1R but upstream of IRAK ... Kinase inactive mutants of TAK1 ( TAK1DN ) and PKR ( PKRDN ) inhibit poly ( dI.dC ) -induced TLR3 mediated NFkappaB activation, suggesting that both of these kinases play important roles in this pathway ... Kinase inactive mutants of TAK1 ( TAK1DN ) and PKR ( PKRDN ) inhibit poly ( dI.dC ) -induced TLR3 mediated NFkappaB activation, suggesting that both of these kinases play important roles in this pathway
Kelly et al., J Biol Chem 2003 : The data also indicate that activation of the transcription factor, nuclear factor kappa B (NF kappa B), may play a role, since the inhibitory effect of LPS and IL-1 beta on LTP was attenuated by the NF kappa B inhibitor, SN50 ; consistently, LPS and IL-1 beta led to activation of NF kappa B in entorhinal cortex ... The data also indicate that activation of the transcription factor , nuclear factor kappa B (NF kappa B) , may play a role, since the inhibitory effect of LPS and IL-1 beta on LTP was attenuated by the NF kappa B inhibitor, SN50 ; consistently, LPS and IL-1 beta led to activation of NF kappa B in entorhinal cortex ... The data also indicate that activation of the transcription factor, nuclear factor kappa B (NF kappa B), may play a role, since the inhibitory effect of LPS and IL-1 beta on LTP was attenuated by the NF kappa B inhibitor, SN50 ; consistently, LPS and IL-1 beta led to activation of NF kappa B in entorhinal cortex
Coffin et al., J Virol 2003 : Additionally, lipid raft association, mediated by transmembrane domains 1 and 2, plays a significant role in LMP-1 activation, and LMP-1 can activate NF-kappaB via an oligomerization/TRAF binding independent mechanism
Basile et al., Mol Cancer Res 2003 : NF-kappaB mediated induction of p21 ( Cip1/Waf1 ) by tumor necrosis factor alpha induces growth arrest and cytoprotection in normal human keratinocytes
Dowds et al., Biochem Biophys Res Commun 2003 (Familial Mediterranean Fever) : Notably, pyrin, the product of the familial Mediterranean fever gene, inhibited cryopyrin mediated apoptosis and NF-kappaB activation by disrupting the cryopyrin-ASC interaction ... Notably, pyrin, the product of the familial Mediterranean fever gene, inhibited cryopyrin mediated apoptosis and NF-kappaB activation by disrupting the cryopyrin-ASC interaction
Chen et al., Cancer Res 2003 (Breast Neoplasms) : Overexpression of a transdominant negative mutant of the inhibitory protein I kappa B alpha results in down-regulation of constitutively active NF-kappa B , induction of DR5 , and tumor necrosis factor receptor (TNFR) 1-associated death domain expression and enhancement of TRAIL sensitivity
Guarini et al., Circulation 2003 (Hypovolemia...) : We investigated whether electrical stimulation ( STIM ) of efferent vagus nerves may suppress nuclear factor (NF)-kappaB activation and the inflammatory cascade in hemorrhagic ( Hem ) shock
Ojaniemi et al., Eur J Immunol 2003 : Inhibition of PI 3-kinase decreased the LPS induced transcriptional activity of NF-kappaB, but it had no effect on the nuclear DNA binding activity of NF-kappaB ... Inhibition of PI 3-kinase decreased the LPS induced transcriptional activity of NF-kappaB , but it had no effect on the nuclear DNA binding activity of NF-kappaB
Nitta et al., Cell Signal 2003 (Cell Transformation, Viral) : Here, we demonstrate that expression of latent membrane protein 1 (LMP1), an Epstein-Barr virus ( EBV ) -encoded membrane protein, triggers programmed cell death in an NF-kappaB dependent manner ... Addition therein of RelA, an active subunit of NF-kappaB, restored the NF-kappaB activation and cell death induction by LMP1, but RelA alone did not induce cell death ... Moreover, LMP1 induced activation of caspase-3 via the activation of NF-kappaB ... Moreover, LMP1 induced activation of caspase-3 via the activation of NF-kappaB
Gu et al., J Biol Chem 2003 : Activation of endothelial cell NF-kappaB by interleukin (IL)-1 constitutes an event critical to the progression of the innate immune response ... We found that RelA, essential for NF-kappaB activation by IL-1 , was associated with the NADPH oxidase adapter protein p47(phox) in yeast two-hybrid, coprecipitation, and in vitro binding studies
Fujioka et al., Oncogene 2003 (Adenocarcinoma...) : Inhibiting NF-kappa B signaling by expressing I kappa B alpha M significantly reduced expression of Bcl-x ( L ) and Bcl-2
Koide et al., J Bone Miner Res 2003 : However, OIP-1/hSca did not affect NF-kappaB activation in these cells
Renier et al., J Diabetes Complications 2003 (Diabetes Mellitus, Type 2...) : The suppressive effect of gliclazide on AGE induced monocyte adhesion to endothelium involves a reduction of cell adhesion molecule mRNA and protein expression and an inhibition of NF-kappaB activation
Giron-Michel et al., Blood 2003 : Differential STAT3, STAT5 , and NF-kappaB activation in human hematopoietic progenitors by endogenous interleukin-15 : implications in the expression of functional molecules
Chiang et al., J Pharm Pharmacol 2003 : We have investigated the role of protein kinase C ( PKC ) and nuclear factor-kappaB (NF-kappaB) in cyclooxygenase-2 (COX-2) expression caused by Staphylococcus aureus lipoteichoic acid in RAW 264.7 macrophages ... Two PKC inhibitors ( Go 6976 and Ro 31-8220 ) and the NF-kappaB inhibitor, pyrrolidine dithiocarbamate ( PDTC ), also attenuated lipoteichoic acid induced COX-2 expression
Sethi et al., Redox Rep 2002 : We propose that oxidation of EPA and its activation of PPARalpha and subsequent inhibition of NF-kappaB is the underlying mechanism for the beneficial effects of fish oil
Yang et al., Am J Respir Crit Care Med 2003 (Respiratory Distress Syndrome, Adult) : We found that nuclear translocation of NF-kappaB in this setting was dependent on the activation of p38 and Akt kinases
Matsuguchi et al., Clin Diagn Lab Immunol 2003 : Furthermore, in HEK293T cells transected with a combination of CD14 and Toll-like receptor 2 (TLR2), NF-kappaB was activated in response to Lactobacillus LTA
Yasui et al., Cancer 2003 (Carcinoma...) : Sulindac inhibited TNF-alpha mediated NF-kappaB activation and greatly sensitized MKN45 and HeLa cell lines to TNF-alpha
Kotani et al., Shock 2003 (Inflammation) : TNFR-1 ( p55 ) and Fas share a death domain which is critical for apoptosis signaling whereas TNFR-p55 and TNFR-2 ( p75 ) can activate NF-kappaB leading to anti-apoptotic proteins expression such as A1 ... TNFR-1 ( p55 ) and Fas share a death domain which is critical for apoptosis signaling whereas TNFR-p55 and TNFR-2 ( p75 ) can activate NF-kappaB leading to anti-apoptotic proteins expression such as A1
Chen et al., Kidney Int 2003 : In contrast, curcumin, an activating protein (AP)-1 inhibitor, attenuated TNF-alpha stimulated phospho-c-Jun levels and fractalkine expression without discernible effects on TNF-alpha induced degradation of I-kappaBalpha or NF-kappaB nuclear translocation ... Neither PD 98059 nor calphostin C affected TNF-alpha induced degradation of I-kappaBalpha or NF-kappaB nuclear translocation ... The present data indicate that TNF-alpha activation of PKCzeta/iota, p42/44 MAPK, c-Jun/AP-1, and p65/NF-kappaB are involved in TNF-alpha stimulated MC fractalkine expression, with the soluble fractalkine mediating in part the TNF-alpha induced monocyte transmigration in vitro
Funakoshi-Tago et al., Eur J Biochem 2003 : We found that IL-1 mediated c-Src activation was required for AP-1 activation, but not for NF-kappa B activation and also revealed that c-Src induced AP-1 activation was enhanced synergistically by the coexpression of TNF receptor associated factor 6 (TRAF6) ... We found that IL-1 mediated c-Src activation was required for AP-1 activation, but not for NF-kappa B activation and also revealed that c-Src induced AP-1 activation was enhanced synergistically by the coexpression of TNF receptor associated factor 6 (TRAF6)
Rakonczay et al., Free Radic Biol Med 2003 (Acute Disease...) : We demonstrated that Arg specifically and dose-dependently induces pancreatitis, activates NF-kappaB ( only the 3 g/kg dose ) and proinflammatory cytokine synthesis, and increases the expressions of HSP60 and HSP72 in the pancreas of rats
Biermer et al., J Virol 2003 : TNF-alpha is shown to suppress hepatitis B virus DNA replication without cell killing by disrupting the formation or stability of cytoplasmic viral capsids through a pathway requiring the NF-kappaB activating inhibitor of kappaB kinase complex IKK-alpha/beta and active transcription factor NF-kappaB ... TNF-alpha is shown to suppress hepatitis B virus DNA replication without cell killing by disrupting the formation or stability of cytoplasmic viral capsids through a pathway requiring the NF-kappaB activating inhibitor of kappaB kinase complex IKK-alpha/beta and active transcription factor NF-kappaB
Erickson et al., J Virol 2003 (Epstein-Barr Virus Infections) : Although lyLMP-1 shares none of LMP-1 's transforming and signal transducing activities, we recently reported that lyLMP-1 can negatively regulate LMP-1 stimulated NF-kappaB activation
Richardson et al., J Virol 2003 (Cell Transformation, Viral) : The increase in STAT1 expression is mediated by the C-terminal activating region 1 ( CTAR-1 ) and/or CTAR-2 domains of LMP-1 and is inhibited by mutant IkappaB, demonstrating a role for NFkappaB in LMP-1 mediated STAT1 expression
Mitsuta et al., Allergy Asthma Proc 2003 (Asthma...) : Our results indicated that TNF-alpha production was induced by D. farinae in PBMCs of patients with atopic asthma by the activation of NF-kappa B via CD23 ... Our results indicated that TNF-alpha production was induced by D. farinae in PBMCs of patients with atopic asthma by the activation of NF-kappa B via CD23
Ebner et al., Blood 2003 : GMCSF activates NF-kappaB via direct interaction of the GMCSF receptor with IkappaB kinase beta ... Functional studies demonstrated GMCSF dependent activation of IkappaB kinase activity in endothelial cells, degradation of IkappaB, and activation of NF-kappaB ... Functional studies demonstrated GMCSF dependent activation of IkappaB kinase activity in endothelial cells, degradation of IkappaB, and activation of NF-kappaB
Abbas et al., J Biol Chem 2003 : We have shown previously that tumor necrosis factor alpha-(TNF) strongly induces osteoclastogenesis of preosteoclasts and do so through activation of the transcription factor, NF-kappaB
Alzuherri et al., Cell Signal 2003 : Calcineurin activates NF-kappaB in skeletal muscle C2C12 cells
Luo et al., Cell Signal 2003 : Furthermore, LPS induced NF-kappaB activation correlated with the degradation of IkappaB-alpha, COX-2 expression, and PGE ( 2 ) synthesis, was inhibited by transfection with dominant negative mutants of NIK and IKK-alpha, but not by IKK-beta ... Furthermore, LPS induced NF-kappaB activation correlated with the degradation of IkappaB-alpha, COX-2 expression, and PGE ( 2 ) synthesis, was inhibited by transfection with dominant negative mutants of NIK and IKK-alpha, but not by IKK-beta ... LPS induced COX-2 expression and PGE ( 2 ) synthesis were completely inhibited by PD98059 ( an inhibitor of MEK1/2 ) and SB203580 ( an inhibitor of p38 MAPK inhibitor ), but these two inhibitors had no effect on LPS induced NF-kappaB activation, indicating that NF-kappaB is activated by LPS independently of activation of p42/p44 MAPK and p38 MAPK pathways in TSMCs. Taken together, these findings suggest that the increased expression of COX-2 correlates with the release of PGE ( 2 ) from LPS challenged TSMCs, at least in part, independently mediated through MAPKs and NF-kappaB signalling pathways
Clermont et al., Cell Signal 2003 : ( 1 ) Although nuclear factor-kappa B (NF-kappaB) was activated by TNF , its inhibition by MG-132 failed to sensitize these cells ... These results indicate that TNF activates NF-kappaB , which does not drive any anti-apoptotic response, and p38, which plays an anti-apoptotic function probably through HSP27 phosphorylation
Guo et al., Mol Cell Biol 2003 (Adenocarcinoma...) : Inhibiting NF-kappa B transcriptional activity in MCF+FIR cells, by using mutant I kappa B alpha, inhibited radioresistance as well as reducing steady-state levels of MnSOD, 14-3-3 zeta, GADD153, cyclin A , and cyclin B1 mRNA ... Inhibiting NF-kappa B transcriptional activity in MCF+FIR cells, by using mutant I kappa B alpha, inhibited radioresistance as well as reducing steady-state levels of MnSOD, 14-3-3 zeta, GADD153, cyclin A, and cyclin B1 mRNA
Kanauchi et al., J Gastroenterol 2003 (Colitis) : GBF has the potential to reduce the epithelial inflammatory response by depressing STAT-3 expression and inhibiting NFkB binding activity
Chen et al., Br J Pharmacol 2003 : ( 5 ) Pretreatment with pentoxifylline ( 0.1-1 mg ml(-1) ) decreased TNF-alpha stimulated fractalkine mRNA and protein expression, which was preceded by a reduction in TNF-alpha activated phosphorylation of PKC, p42/44 MAPK and c-Jun as well as degradation of I-kappaBalpha and p65/NF-kappaB nuclear translocation
Lee et al., J Biol Chem 2003 : Furthermore, LPS induced NF-kappa B activation assessed by the inhibitory protein of NF-kappa B ( I kappa B ) degradation and p50 nuclear translocation was diminished in cells subjected to prolonged treatment with the low concentration of 15d-PGJ2 ... Likewise, HO inhibitor and CO scavenger were effective in abolishing the inhibitory effects of 15d-PGJ2 on NF-kappa B activation induced by LPS
Shigeno et al., Oncogene 2003 (Carcinoma, Hepatocellular...) : On the other hand, TRAIL activated NF-kappa B composed of RelA-p50 heterodimer, a key transcription factor regulating cell survival, in HuH-7 and HepG2 cells ... However, IFN-alpha pretreatment repressed the TRAIL mediated activation of NF-kappaB and decreased its transcriptional activity in HuH-7 but not in HepG2 cells
Chu et al., Dig Dis Sci 2003 (Helicobacter Infections...) : Role of NF-kappaB and AP-1 on Helicobater pylori induced IL-8 expression in AGS cells ... IL-8 may be regulated by oxidant-sensitive transcription factors, NF-kappaB , and AP-1 ... The present study aims to investigate whether H. pylori induced IL-8 expression is regulated by NF-kappaB and AP-1 in gastric epithelial AGS cells and whether this transcriptional regulation of IL-8 is inhibited by N-acetylcysteine (NAC)
de Erausquin et al., Mol Pharmacol 2003 : Treatment of mesencephalic cultures with alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid ( AMPA ) resulted in a number of changes that occurred selectively in dopaminergic neurons, including persistent elevation in intracellular Ca ( 2+ ) monitored with Fura-2, and a significant increase in intramitochondrial oxidation of dihydrorhodamine 123, probably associated with transient increase of mitochondrial permeability, cytochrome c release, nuclear translocation of NF kappa B , and transcriptional activation of the oncogene p53
Dasgupta et al., J Immunol 2003 (Acute Disease...) : However, pretreatment of MBP primed T cells with NaPA markedly inhibited its ability to induce microglial expression of iNOS and activation of NF-kappaB
Sonoki et al., Metabolism 2003 (Arteriosclerosis...) : Moreover, LDL isolated from diabetics contained more lyso-PC than that from nondiabetic subjects, and induced higher MCP-1 mRNA expression and NF-kappaB activity in HUVEC ... Preincubation with 4-ethyl-2-hydroxyimino-5-nitro-3-hexenamide, a NO donor, abrogated increased expression of MCP-1 mRNA and high NF-kappaB activity induced by PLA2 treated LDL and by LDL isolated from diabetic patients
Wan et al., Immunity 2003 : The inhibition of NFkappaB in antigen stimulated T cells results in apoptosis but does not inhibit E2F activation and S phase entry
Hodge et al., Cancer Res 2003 (Adenocarcinoma...) : Transfection of these highly invasive cells with dominant negative RhoA N19 or treatment with 1.0 micro g/ml RhoA inhibitor C3 exoenzyme demonstrated that RhoA activity was necessary for both NF-kappaB activity and cellular invasion of a Matrigel reconstituted basement membrane ... RhoA activity was therefore both necessary and sufficient for the elevated NF-kappaB , invasion, and motility activities of the PC-3 highly invasive cells
Mayo et al., J Biol Chem 2003 (Carcinoma, Non-Small-Cell Lung...) : Because the anti-apoptotic transcription factor NF-kappa B has been shown to be under the control of HDAC mediated repression, we analyzed whether HDAC inhibitors activated NF-kappa B in NSCLC cells ... HDAC inhibitors effectively stimulated endogenous NF-kappa B-dependent gene expression by up-regulating IL-8, Bcl-XL, and MMP-9 transcripts
Ferri et al., J Biol Chem 2003 : Thus, polymerized collagen induces NF-kappaB dependent expression of MMP1 and alpha2beta1 integrin , that are required for smooth muscle extracellular matrix remodeling ... Thus, polymerized collagen induces NF-kappaB dependent expression of MMP1 and alpha2beta1 integrin, that are required for smooth muscle extracellular matrix remodeling
Yuksel et al., J Pharmacol Exp Ther 2003 : These observations strongly suggest that gabexate mesilate inhibited LPS induced TNF-alpha production in human monocytes by inhibiting activation of both NF-kappaB and AP-1
Donepudi et al., Cancer Immunol Immunother 2003 : Although AP-1 and NF-kappa B are considered redox-sensitive transcription factors, L-PAM led only to activation of NF-kappa B that bound specifically to a probe containing the corresponding binding site in the B7-1 gene
Lu et al., J Neurosurg 2003 (Wounds and Injuries) : Both NF-kappaB regulated COX-2 expression and inflammation play an important role in the pathogenesis of surgery associated paraspinal muscle injury
Stehlik et al., Biochem J 2003 : In gene transfer experiments, POP1/ASC2 suppressed cytokine mediated NF-kappa B activation similar to other PAAD-family proteins previously tested ... In gene transfer experiments, POP1/ASC2 suppressed cytokine mediated NF-kappa B activation similar to other PAAD-family proteins previously tested ... In gene transfer experiments, POP1/ASC2 suppressed cytokine mediated NF-kappa B activation similar to other PAAD-family proteins previously tested
Håversen et al., Cell Immunol 2002 : Moreover, by electrophoretic mobility shift assay ( EMSA ) analysis LF decreased the LPS induced binding of NF-kappa B to the TNF-alpha promoter
Carter et al., J Biol Chem 2003 : Although its precise mechanism of action remains unknown, IKKgamma is phosphorylated in concert with the induction of NF-kappaB by the viral oncoprotein Tax and the proinflammatory cytokine tumor necrosis factor alpha (TNF)
O'Reilly et al., Biochem Biophys Res Commun 2003 : Furthermore full length and C-terminal A20 showed similar regulatory effects on MEKK-1 activation of NF-kappa B and AP-1 and induction of IL-8
Puig-Kröger et al., J Leukoc Biol 2003 : Evaluation of MDDC maturation parameters [ expression of adhesion and costimulatory molecules, receptor mediated endocytosis, allogeneic T cell activation, production of tumor necrosis factor alpha, interleukin (IL)-6 and IL-12 p70, and nuclear factor (NF)-kappaB activation ] revealed that currently used PD solutions differentially inhibit the lipopolysaccharide (LPS) induced maturation of MDDC, an inhibition that correlated with their ability to impair the LPS stimulated NF-kappaB activation
Hettmann et al., Immunol Lett 2003 : Transgenic mice in which NF-kappaB activity is inhibited by the expression of a dominant negative form of IkappaB-alpha ( mIkappaB-alpha mice ) have drastically diminished numbers of CD8 ( + ) memory-phenotype cells
Hirano et al., J Hepatol 2003 (Carcinoma, Hepatocellular...) : Tumor necrosis factor alpha (TNF-alpha) induced RANTES chemokine expression via activation of NF-kappaB and p38 MAP kinase : roles of TNF-alpha in alcoholic liver diseases ... TNF-alpha clearly up-regulated RANTES expression in a time dependent fashion and induced DNA binding activity of NF-kappaB
El Bekay et al., Blood 2003 (Calcium Signaling...) : Ang II increased cytosolic Ca2+ levels released mainly from calcium stores enhanced the synthesis de novo and activity of calcineurin, and stimulated the DNA binding activity of the transcription factor NF-kappaB in cultured human neutrophils
Oya et al., Carcinogenesis 2003 (Carcinoma, Renal Cell...) : The serum CRP elevation correlated with the increased NF-kappa B activation , and therefore NF-kappa B may be a causative transcription factor of inflammatory paraneoplastic syndrome
Yang et al., J Biol Chem 2003 : Quantitative analyses showed that RelA-NF-kappaB induced IkappaBalpha binding is regulated through inhibitor retention and phosphorylation ... The data show that RelA regulation of NF-kappaB-IkappaBalpha complex formation is IkappaBalpha phosphorylation dependent and that IkappaBalpha/NF-kappaB binding is dynamic and determined by concentration of the subunits ... The data show that RelA regulation of NF-kappaB-IkappaBalpha complex formation is IkappaBalpha phosphorylation dependent and that IkappaBalpha/NF-kappaB binding is dynamic and determined by concentration of the subunits
Liu et al., J Biol Chem 2003 : TRAP expression in osteoclasts is regulated by receptor activator of NF-kappaB ( RANKL ), a potent activator of osteoclast differentiation
Compton et al., J Virol 2003 (Inflammation) : Induction of inflammatory cytokines is mediated via TLR2 dependent activation of NF-kappa B
Devchand et al., FASEB J 2003 (Inflammation...) : hALX transfected HEK293 cells transmitted LXA4 signals that inhibit TNFalpha induced NFkappaB activation
Parhar et al., Immunology 2003 : However, the precise inter-relationship between the activation of p38 MAPK and activation of the transcription factor NF-kappaB in the intestinal epithelial cell ( IEC ) system, remains unknown ... It is concluded that p38 MAPK influences the expression of chemokines in intestinal epithelial cells, through an effect upon the activation of the chemokine promoter, and does not directly involve the activation of the transcription factor NF-kappaB
Agrawal et al., Immunology 2003 : They raise the possibility, not as yet established, that NF-kappaB activation may be responsible for the synergistic effect of IL-1beta on IL-6 induced CRP expression
Yang et al., Mol Ther 2003 (Myocardial Reperfusion Injury...) : These findings support the notion that both mitochondrial and cytoplasmic derived SOD induce changes in AP-1 and NF kappa B activity, creating an antiapoptotic microenvironment within cardiomyocytes that affords protection following I/R injury
Sano et al., Cytokine 2003 : Effects of secretory leukocyte protease inhibitor on the tumor necrosis factor-alpha production and NF-kappaB activation of lipopolysaccharide stimulated macrophages ... 1/2 SLPI weakly increased Mphi TNF-alpha production in response to LPS signaling and potentiated the LPS induced activation of NF-kappaB , especially the binding of p65-p50 heterodimers to the DNA kappaB sites, suggesting that LPS-hyporesponsiveness of Lps ( d ) Mphis is not due to the overexpression of SLPI ... 1/2 SLPI weakly increased Mphi TNF-alpha production in response to LPS signaling and potentiated the LPS induced activation of NF-kappaB , especially the binding of p65-p50 heterodimers to the DNA kappaB sites, suggesting that LPS-hyporesponsiveness of Lps ( d ) Mphis is not due to the overexpression of SLPI
Dechend et al., Circulation 2003 (Pre-Eclampsia) : To elucidate their role further, we studied the effects of AT1-AA on reactive oxygen species ( ROS ), NADPH oxidase expression, and nuclear factor-kappaB (NF-kappaB) activation ... We next tested if AT1-AA lead to NF-kappaB activation in VSMC and trophoblasts ... VSMC from p47phox-/- mice showed markedly reduced ROS generation and NF-kappaB activation in response to Ang II and AT1-AA ... VSMC from p47phox-/- mice showed markedly reduced ROS generation and NF-kappaB activation in response to Ang II and AT1-AA ... Furthermore, NF-kappaB was activated and I-kappaBalpha reduced in placentas from preeclamptic women
Secchiero et al., Circulation 2003 (MAP Kinase Signaling System) : Conversely, TRAIL did not activate NF-kappaB or affect the surface expression of the inflammatory markers E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1
Zheng et al., J Exp Med 2003 : Our results further indicate that NF-kappaB regulates TCR induced expression of antiapoptotic Bcl-2 family members ... In contrast, our results indicate a lack of involvement of NF-kappaB in both IL-2 and Akt induced survival pathways
Park et al., Biochem Biophys Res Commun 2003 (Insulin Resistance...) : Oleic acid induces endothelin-1 expression through activation of protein kinase C and NF-kappa B
Agarwal et al., FASEB J 2003 : This leads to sustained suppression of IL-1beta induced NF-kappaB transcriptional regulation of proinflammatory genes
Yan et al., Restor Neurol Neurosci 1998 : Abeta-RAGE interaction also activated NF-kappaB , resulting in neuronal up-regulation of macrophage-colony stimulating factor ( M-CSF ) which also induced microglial migration
Tang et al., J Hand Surg Am 2003 (Tendon Injuries) : Expression levels of type I collagen and NF-kappaB genes of tenocytes were increased significantly by bFGF ... Results of this study show that expression of type I collagen and NF-kappaB genes is promoted manifestly by bFGF
Burke et al., J Neurobiol 2003 : p75 neurotrophin receptor mediates neurotrophin activation of NF-kappa B and induction of iNOS expression in P19 neurons ... p75 neurotrophin receptor mediates neurotrophin activation of NF-kappa B and induction of iNOS expression in P19 neurons
Faveeuw et al., Eur J Immunol 2003 : Electrophoretic mobility shift assays demonstrated that PGD ( 2 ) affects NF-kappa B activation through ( the ) DP1 independent pathway ( s )
Haller et al., J Biol Chem 2003 (Bacteroides Infections) : Transforming growth factor-beta 1 inhibits non-pathogenic Gram negative bacteria induced NF-kappa B recruitment to the interleukin-6 gene promoter in intestinal epithelial cells through modulation of histone acetylation ... Interestingly, TGF-beta1 inhibited B. vulgatus- and lipopolysaccharide (LPS) induced NF-kappaB transcriptional activity as well as interleukin-6 (IL-6) mRNA accumulation and protein secretion in IEC ... Interestingly, TGF-beta1 inhibited B. vulgatus- and lipopolysaccharide (LPS) induced NF-kappaB transcriptional activity as well as interleukin-6 (IL-6) mRNA accumulation and protein secretion in IEC ... The inhibitory effect of TGF-beta1 is mediated independently of B. vulgatus/LPS induced IkappaBalpha, Akt, and RelA phosphorylation as well as NF-kappaB DNA binding activity ... Adenoviral delivery of Smad7 and dominant negative Smad3 ( SmadDelta3 ) reversed the TGF-beta1 mediated inhibition of NF-kappaB transcriptional activity and NF-kappaB recruitment to the IL-6 promoter
Higuchi et al., J Biol Chem 2003 : Expression of a dominant negative mutant of ASK1 eliminated the RacV12 induced NF-kappaB activation and the biochemical and morphological hypertrophic responses, whereas expression of a dominant negative form of Rac1 attenuated phenylephrine induced activation of ASK1 and NF-kappaB and cardiac myocyte hypertrophy
Kim et al., Biol Pharm Bull 2003 : Furthermore, SCM inhibited the LPS induced DNA binding activity of nuclear factor-kappaB (NF-kappaB) , which was associated with decreased p65 protein levels in the nucleus ... These results suggest that SCM inhibits LPS induced iNOS and COX-2 expression by blocking NF-kappaB activation ... These results suggest that SCM inhibits LPS induced iNOS and COX-2 expression by blocking NF-kappaB activation
Hinata et al., Oncogene 2003 (Inflammation) : In keratinocytes, but not in fibroblasts, NF-kappa B induced p21 ( CIP1 ), which was sufficient to inhibit growth of both cell types ... Levels of growth inhibitory factor (GIF) , in contrast, were increased by NF-kappa B in both settings but inhibited growth only in keratinocytes
Kim et al., Biol Chem 2003 : Pyrrolidine dithiocarbamate ( PDTC ) has been shown to have unique reciprocal activities in activating AP-1 and inhibiting NF-kappaB , two oxidative stress-sensitive transcription factors
Ruan et al., J Huazhong Univ Sci Technolog Med Sci 2002 : Furthermore, EMSA showed that anisodamine completely abolished LPS induced NF-kappa B DNA binding activity in nuclear extracts from HUVECs treated with LPS together with anisodamine
Chiarugi et al., J Neurochem 2003 (Neurodegenerative Diseases) : Our findings build on prior published reports in PARP-1 null mice and highlight the importance of PARP-1 enzymatic activity in transcriptional control during glial activation, identifying PARP-1 activity dependent regulation of NF-kappaB as a novel pharmacological target for therapeutic intervention in the treatment of acute and chronic neurodegenerative disorders
Heneka et al., J Neurochem 2003 (Encephalitis) : DSP4-treatment reduced basal levels of nuclear factor kappa B (NF-kappaB) inhibitory IkappaB proteins, and of heat shock protein ( HSP ) 70
Ogura et al., Genomics 2003 (Crohn Disease) : Expression of mouse Nod2 activated NF-kappaB and conferred responsiveness to bacterial components, an activity that was deficient in mutants corresponding to those associated with susceptibility to Crohn 's disease
Largo et al., Osteoarthritis Cartilage 2003 (Osteoarthritis, Knee) : Glucosamine inhibits IL-1beta induced NFkappaB activation in human osteoarthritic chondrocytes
Suzuki et al., J Immunol 2003 : IRAK-4 ( -/- ) Th1 cells also do not exhibit NF-kappaB activation or IkappaB degradation in response to IL-18
Gingery et al., J Cell Biochem 2003 (MAP Kinase Signaling System) : PI3K inhibition also blocked MEK1/2, ERK1/2, and AKT phosphorylation and NFkappaB activation in purified osteoclasts
Chandrasekar et al., Biochem Biophys Res Commun 2003 : TNF-alpha induced rapid and sustained activation of NF-kappaB whereas H ( 2 ) O ( 2 ) induced delayed and transient activation
Ahmed-Choudhury et al., Mol Biol Cell 2003 : CD40 ligation induced a rise in NF-kappaB activity in hepatocytes, which peaked at 2 h and returned to baseline by 24 h ; however, IHEC CD40 ligation resulted in a sustained up-regulation of NF-kappaB ( > 24 h )
Li et al., J Pharmacol Sci 2003 (Bacterial Infections...) : Among those signaling pathways, activation of NF-kappaB leads to up-regulation of IL-1beta, IL-8 and TNF-alpha , mucin MUC2 and Toll-like receptor 2 (TLR2), whereas activation of p38 MAP kinase mediates not only up-regulation of inflammatory mediators and mucin MUC5AC but also down-regulation of TLR2 ... Among those signaling pathways, activation of NF-kappaB leads to up-regulation of IL-1beta , IL-8 and TNF-alpha, mucin MUC2 and Toll-like receptor 2 (TLR2), whereas activation of p38 MAP kinase mediates not only up-regulation of inflammatory mediators and mucin MUC5AC but also down-regulation of TLR2 ... Among those signaling pathways, activation of NF-kappaB leads to up-regulation of IL-1beta, IL-8 and TNF-alpha, mucin MUC2 and Toll-like receptor 2 (TLR2), whereas activation of p38 MAP kinase mediates not only up-regulation of inflammatory mediators and mucin MUC5AC but also down-regulation of TLR2
Estrov et al., Blood 2003 (Acute Disease...) : Resveratrol blocks interleukin-1beta induced activation of the nuclear transcription factor NF-kappaB , inhibits proliferation, causes S-phase arrest, and induces apoptosis of acute myeloid leukemia cells ... It also suppressed the IL-1beta induced activation of transcription factor nuclear factor kappaB (NF-kappaB) , which modulates an array of signals controlling cellular survival, proliferation, and cytokine production
Jewett et al., Hum Immunol 2003 : Thus, NFkappaB regulates inversely the expression of ICAM-1 and MHC class I antigens on HEp2 tumor cells and this may contribute to the resistance of these cells to NK cell mediated cytotoxicity
Zernecke et al., FASEB J 2003 : Only at high concentrations, cPG enhanced TNF-alpha induced cell death and inhibited TNF-alpha induced IkappaB-alpha kinase (IKK) activation, IkappaB-alpha degradation, and NF-kappaB/p65 translocation, while promoting AP-1/c-jun phosphorylation
Fitzgerald et al., Nat Immunol 2003 (Virus Diseases) : Viral infection, or the binding of double stranded RNA to Toll-like receptor 3 , results in the coordinate activation of IRF3 and NF-kappaB
Takeuchi et al., J Neurochem 2003 (Neuroblastoma) : Stimulation with forskolin and overexpression of constitutively active PKA suppressed NF-kappaB activity, likely due to D1R stimulation ... These results indicate that D2R induced NF-kappaB activation through ERK may be involved in activation of the NCAM promoter, and additionally that other protein kinases such as CaM KII and p38 MAPK also regulate NCAM expression ... These results indicate that D2R induced NF-kappaB activation through ERK may be involved in activation of the NCAM promoter, and additionally that other protein kinases such as CaM KII and p38 MAPK also regulate NCAM expression
Takada et al., J Biol Chem 2003 : In this report, we used macrophages derived from wild type ( wt ) mice and from mice null for the type 1 receptor ( p60-/- ), the type 2 receptor ( p80-/- ), or both ( p60-/- p80-/- ) to investigate the effect of these receptors on LPS mediated activation of NF-kappaB , mitogen activated protein kinases, and apoptosis ... LPS activated NF-kappaB by 3-4-fold in wt cells but by 9-10-fold in p60-/-, p80-/-, and p60-/- p80-/- macrophages
Yeo et al., J Biol Chem 2003 : Myeloid differentiation factor 88-dependent transcriptional regulation of cyclooxygenase-2 expression by CpG DNA : role of NF-kappaB and p38 ... In contrast, overexpression of DN-IRAK1 or DN-TRAF6 only partially inhibited CpG DNA induced Cox-2 promoter activity and NF-kappaB activation, indicating the presence of additional signaling modulators downstream of MyD88 ... In contrast, overexpression of DN-IRAK1 or DN-TRAF6 only partially inhibited CpG DNA induced Cox-2 promoter activity and NF-kappaB activation, indicating the presence of additional signaling modulators downstream of MyD88 ... In addition, Cox-2 promoter-luciferase reporters with alterations in predicted cis acting transcriptional regulatory elements revealed that C/EBP, Ets-1, NF-kappaB , and CREB binding sites are essential for optimal Cox-2 expression in response to CpG DNA ... Conclusively, these results demonstrate that endosomal DNA processing and TLR9/MyD88 dependent activation of NF-kappaB and p38 are required for transcriptional regulation of Cox-2 expression induced by CpG DNA, and suggest that interleukin-1 receptor associated kinase and/or TRAF6 may be a diverging point for NF-kappaB activation in response to CpG DNA in RAW264.7 cells ... Conclusively, these results demonstrate that endosomal DNA processing and TLR9/MyD88 dependent activation of NF-kappaB and p38 are required for transcriptional regulation of Cox-2 expression induced by CpG DNA, and suggest that interleukin-1 receptor associated kinase and/or TRAF6 may be a diverging point for NF-kappaB activation in response to CpG DNA in RAW264.7 cells ... Conclusively, these results demonstrate that endosomal DNA processing and TLR9/MyD88 dependent activation of NF-kappaB and p38 are required for transcriptional regulation of Cox-2 expression induced by CpG DNA, and suggest that interleukin-1 receptor associated kinase and/or TRAF6 may be a diverging point for NF-kappaB activation in response to CpG DNA in RAW264.7 cells
Bian et al., Exp Eye Res 2003 (Translocation, Genetic) : This study investigates chemokine secretion induced by co-culture of hRPE cells and monocytes and illustrates the roles of p38 kinase, ERK, JNK/SAPK and NF-kappaB inducing kinase signaling pathways for hRPE IL-8 and MCP-1 secretion induced in hRPE by co-culture with monocytes ... This study investigates chemokine secretion induced by co-culture of hRPE cells and monocytes and illustrates the roles of p38 kinase, ERK, JNK/SAPK and NF-kappaB inducing kinase signaling pathways for hRPE IL-8 and MCP-1 secretion induced in hRPE by co-culture with monocytes ... These results suggest that activation of DEX-sensitive, CSA-resistant MEK/ERK and p38 pathways, and activation of NF-kappaB , PKC, and PTK are essential for IL-8 and MCP-1 expression by hRPE cells ... These results suggest that activation of DEX-sensitive, CSA-resistant MEK/ERK and p38 pathways, and activation of NF-kappaB , PKC, and PTK are essential for IL-8 and MCP-1 expression by hRPE cells ... These results suggest that activation of DEX-sensitive, CSA-resistant MEK/ERK and p38 pathways, and activation of NF-kappaB , PKC, and PTK are essential for IL-8 and MCP-1 expression by hRPE cells ... These results suggest that activation of DEX-sensitive, CSA-resistant MEK/ERK and p38 pathways, and activation of NF-kappaB , PKC, and PTK are essential for IL-8 and MCP-1 expression by hRPE cells
Nomura et al., Protein Expr Purif 2003 : In addition, the rsCD14 protein could mediate the LPS mediated activation of NF-kappaB in human embryonic kidney 293 cells transfected with Toll-like receptor 4 and MD-2, indicating that the purified protein is biologically active
Kerzic et al., Toxicology 2003 : We demonstrate in both cell types that TNF-alpha activates NF-kappaB , and HQ exposure inhibits activation of NF-kappaB by TNF-alpha in a dose dependent manner
Zhou et al., Toxicological sciences : an official journal of the Society of Toxicology 2003 : Alcohol consumption decreases IL-2 induced NF-kappaB activity in enriched NK cells from C57BL/6 mice ... In this study we show by electrophoretic mobility-shift assay ( EMSA ) that enriched splenic NK cells from EtOH consuming C57BL/6 mice exhibit reduced NF-kappaB and AP-1 binding activity in response to IL-2 stimulation as compared to the water drinking mice ... Pyrrolidine dithiocarbamate ( PDTC ) and N-tosyl-L-phenylalanine chloromethyl ketone ( TPCK ) blocked NFkappaB and AP-1 binding activity in nuclear extracts of IL-2 stimulated NK cells in an EMSA and also inhibited the IL-2 induced expression of perforin, granzyme A , and granzyme B gene expression in enriched NK cells ... Pyrrolidine dithiocarbamate ( PDTC ) and N-tosyl-L-phenylalanine chloromethyl ketone ( TPCK ) blocked NFkappaB and AP-1 binding activity in nuclear extracts of IL-2 stimulated NK cells in an EMSA and also inhibited the IL-2 induced expression of perforin, granzyme A, and granzyme B gene expression in enriched NK cells
Ferlito et al., J Endotoxin Res 2002 : Neither PTx nor PP2 inhibited LPS induced activation of interleukin receptor activated kinase ( IRAK ) or inhibited translocation of NF-kappaB ... Neither PTx nor PP2 inhibited LPS induced activation of interleukin receptor activated kinase ( IRAK ) or inhibited translocation of NF-kappaB ... Neither PTx nor PP2 inhibited LPS induced activation of interleukin receptor activated kinase ( IRAK ) or inhibited translocation of NF-kappaB ... Neither PTx nor PP2 inhibited LPS induced activation of interleukin receptor activated kinase ( IRAK ) or inhibited translocation of NF-kappaB ... However, PP2 inhibited LPS induced NF-kappaB transactivation of a luciferase reporter gene construct in a concentration dependent manner ... However, PP2 inhibited LPS induced NF-kappaB transactivation of a luciferase reporter gene construct in a concentration dependent manner
Ovesen et al., Acta Otolaryngol 2003 : LPS caused significant enhancement of NF-kappaB
Costanzo et al., J Cell Physiol 2003 : However, NFkappaB stimulation by AII differs from that of TNF-alpha since a TNF-receptor associated factor 2 ( TRAF-2 ) dominant negative mutant does not prevent AII mediated NFkappaB activation ... In analogy with TNF-alpha dependent activation of NFkappaB , treatment with either the anti-oxidant N-acetyl cysteine (NAC) or the cyclooxygenase (COX) inhibitor acetyl salicylic acid ( aspirin ), but not indometacin, prevents the induction of NFkappaB dependent transcription by AII
Delfino et al., Mol Cell Endocrinol 2003 : NF-kappaB and TNF-alpha stimulate androgen receptor expression in Sertoli cells ... Moreover, TNF-alpha , a secretory product of round spermatids, stimulated NF-kappaB binding to the AR promoter, induced AR promoter activity, and increased endogenous AR expression in primary cultures of Sertoli cells
Andriollo et al., Arch Biochem Biophys 2003 (Carcinoma, Small Cell...) : Activation of NF-kappaB in response to tumor necrosis factor was intact in both cell lines
Kim et al., FEBS Lett 2003 : NF-kappaB and AP-1 regulate activation dependent CD137 ( 4-1BB ) expression in T cells
Ueda et al., Cancer Lett 2003 (Stomach Neoplasms) : Blocking of NF-kappaB activation enhances the tumor necrosis factor alpha induced apoptosis of a human gastric cancer cell line
Matsumura et al., Immunology 2003 : Although TLR2 mRNA stability was not affected, these treatments enhanced NF-kappaB activity and TLR2 gene transcription simultaneously ... The up-regulation of TLR2 transcription in response to these reagents was completely inhibited by blocking the NF-kappaB activation pathway, demonstrating a pivotal role of NF-kappaB activation in the regulation of hepatocyte TLR2 transcription
Henkler et al., J Biol Chem 2003 : The role of tumor necrosis factor (TNF) receptor associated factor ( TRAF)-1 in NF-kappaB activation by various members of the TNF receptor family is not well understood, and conflicting data have been published ... Here, we show that TRAF1 differentially affects TRAF2 recruitment and activation of NF-kappaB by members of the TNF receptor family ... The role of tumor necrosis factor (TNF) receptor associated factor ( TRAF)-1 in NF-kappaB activation by various members of the TNF receptor family is not well understood, and conflicting data have been published ... Here, we show that TRAF1 differentially affects TRAF2 recruitment and activation of NF-kappaB by members of the TNF receptor family
Derudder et al., J Biol Chem 2003 : Using various deficient mouse embryonic fibroblast cells, we have compared the signaling pathways leading to NF-kappaB induction in response to TNF-alpha and LTbetaR activation
Eng et al., Microvasc Res 2003 : Further supporting NF-kappaB regulation of PDGF-B, activation of NF-kappaB by interleukin (IL)-1beta resulted in increased PDGF-B transcript levels ... Further supporting NF-kappaB regulation of PDGF-B, activation of NF-kappaB by interleukin (IL)-1beta resulted in increased PDGF-B transcript levels ... Further supporting NF-kappaB regulation of PDGF-B, activation of NF-kappaB by interleukin (IL)-1beta resulted in increased PDGF-B transcript levels
Takada et al., J Biol Chem 2003 : Unexpectedly, however, like TNF induced activation, H2O2 induced NF-kappaB activation was blocked by the calpain inhibitor N-Ac-Leu-Leu-norleucinal , suggesting that a proteosomal pathway was involved ... We present several lines of evidence to suggest that the Syk protein-tyrosine kinase is involved in H2O2 induced NF-kappaB activation ... First, H2O2 activated Syk in KBM-5 cells ; second, H2O2 failed to activate NF-kappaB in cells that do not express Syk protein ; third, overexpression of Syk increased H2O2 induced NF-kappaB activation ; and fourth, reduction of Syk transcription using small interfering RNA inhibited H2O2 induced NF-kappaB activation
Nagasaki et al., Int J Cancer 2003 (Pancreatic Neoplasms) : Leucine-zipper protein , LDOC1, inhibits NF-kappaB activation and sensitizes pancreatic cancer cells to apoptosis ... The activation of NF-kappaB through ligand induced stimulation by tumor necrosis factor-alpha (TNF-alpha) or phorbol 12-myristate 13-acetate ( PMA ) was also inhibited by transient expression of LDOC1 in a dose dependent manner ... The activation of NF-kappaB through ligand induced stimulation by tumor necrosis factor-alpha (TNF-alpha) or phorbol 12-myristate 13-acetate ( PMA ) was also inhibited by transient expression of LDOC1 in a dose dependent manner
Park et al., DNA Cell Biol 2003 : Regulatory effect of SOCS on NF-kappaB activity in murine monocytes/macrophages ... The effects of increased SOCS-3 expression upon IL-10 regulation of NF-kappaB activation have not yet been demonstrated ... Here we examined the effects of SOCS-3 on NF-kappaB activity ... SOCS-3 did not induce any alterations in NF-kappaB activity induced by LPS or TNF-alpha ... SOCS-3 did not induce any alterations in NF-kappaB activity induced by LPS or TNF-alpha
Pollet et al., Blood 2003 : Bacterial lipopolysaccharide directly induces angiogenesis through TRAF6 mediated activation of NF-kappaB and c-Jun N-terminal kinase
Dai et al., Blood 2003 : While TGFbeta had a modest effect on transcription of the KC and MIP-2 mRNAs as measured by nuclear run-on, it had no effect on LPS stimulated luciferase expression driven by the KC promoter nor on the activation of nuclear factor kappaB (NFkappaB) DNA binding activity and transactivation function
McDermott et al., Invest Ophthalmol Vis Sci 2003 : The NFkappaB inhibitors pyrrolidinedithiocarbamate ( PDTC ; 100 microM ), caffeic acid phenethyl ester ( CAPE ; 90 microM ), and MG-132 ( 25 microM ), blocked IL-1beta stimulated expression of hBD-2
Tan et al., Sheng Li Xue Bao 2003 (Inflammation) : The effects of NF-kappaB inhibitor MG-132 on ICAM-1 expression were also observed ... It is suggested that a developing and intensive airway inflammation correlates closely with a persistent expression of ICAM-1 and repeated activation of NF-kappaB
Li et al., Am J Physiol Lung Cell Mol Physiol 2003 (MAP Kinase Signaling System) : Stretch also induced NF-kappaB inducing kinase (NIK) activation, and inhibition of NF-kappaB attenuated IL-8 mRNA expression and IL-8 production
Pellegatta et al., Am J Clin Nutr 2003 : The nuclear appearance of p50-NFkappaB and p65-NFkappaB acutely induced by TNF-alpha was not modified by resveratrol but was increased after overnight incubation with resveratrol alone or in combination with TNF-alpha
Pierce et al., Diabetes 2003 (Diabetes Mellitus, Type 1) : Strong T1D protection mediated at the beta-cell level characterized DL704/NOD mice lacking the E3 gp19K gene suppressing MHC class I expression but retaining the 10.4K, 14.5K, and 14.7K genes inhibiting Fas- or TNF-alpha induced apoptosis and TNF-alpha induced NF-kB activation
Chang et al., Diabetes 2003 (Cell Transformation, Viral...) : Recent studies incriminating tumor necrosis factor (TNF)-alpha as the final effector in pancreatic beta-cell death in type 1 diabetes underscore the potential role of TNF-alpha dependent NF-kappaB activation as an important modulator of pancreatic beta-cell death in autoimmune diabetes ... The NF-kappaB DNA binding nuclear complex activated by TNF-alpha contained both the p65 and p50 subunit ... IFN-gamma pretreatment did not affect TNF-alpha induced NF-kappaB activation
Satoh et al., Pancreas 2003 (Acute Disease...) : In vitro, the lipopolysaccharide (LPS) treatment of the PBMCs from the control subjects and patients with mild pancreatitis induced further activation of the NF-kappaB ... Patients who had persistently high NF-kappaB activities, a reduced response of NF-kappaB to LPS , and a low p50p65 : p50p50 ratio after LPS stimulation at 14 days developed serious systemic complications in the later clinical course
Paik et al., Hepatology 2003 (Liver Cirrhosis) : Both LPS- and lipid A-induced NF-kappaB activation is blocked by preincubation with either anti-TLR4 blocking antibody ( HTA125 ) or Polymyxin B. Lipid A induces NF-kappaB activation in HSCs from TLR4-sufficient ( C3H/OuJ ) mice but not from TLR4-deficient ( C3H/HeJ ) mice
Landowski et al., Oncogene 2003 (Multiple Myeloma) : This DNA binding activity consisted primarily of RelB-p50 heterodimers, which was distinct from the NF-kappa B activation of TNF alpha
Bin et al., J Biol Chem 2003 : Overexpression of TIRP activates NF-kappaB and potentiates IL-1 receptor mediated NF-kappaB activation ... Moreover, TIRP mediated NF-kappaB activation is inhibited by dominant negative mutants of IRAK, IRAK-2, TRAF6 , and IKKbeta ... Moreover, TIRP mediated NF-kappaB activation is inhibited by dominant negative mutants of IRAK, IRAK-2 , TRAF6, and IKKbeta ... Moreover, TIRP mediated NF-kappaB activation is inhibited by dominant negative mutants of IRAK, IRAK-2, TRAF6, and IKKbeta
Harper et al., J Biol Chem 2003 : TRADD also binds two other adaptors receptor interacting protein ( RIP ) and TNF-receptor associated factor 2 (TRAF2), which are required for TNF induced NF-kappaB and c-Jun N-terminal kinase activation, respectively
Yoneda et al., J Med Microbiol 2003 : Following infection of HEp-2 cells with C. pneumoniae, NF-kappa B was activated, COX-2 was induced and PGE ( 2 ) was elevated ... Following infection of HEp-2 cells with C. pneumoniae, NF-kappa B was activated, COX-2 was induced and PGE ( 2 ) was elevated
Hierholzer et al., Arch Orthop Trauma Surg 2003 (Shock, Hemorrhagic) : Shock animals demonstrated increased mRNA levels of interleukin (IL)-6 and intercellular adhesion molecule (ICAM)-1 and increased activation of the transcription factors nuclear factor kappa B (NF-kappa B) and signal transducers and activators of transcription 3 ( Stat3 ) ... Shock animals demonstrated increased mRNA levels of interleukin (IL)-6 and intercellular adhesion molecule (ICAM)-1 and increased activation of the transcription factors nuclear factor kappa B (NF-kappa B) and signal transducers and activators of transcription 3 ( Stat3 )
Jefferies et al., J Biol Chem 2003 : In addition, a dominant negative form of Btk inhibited TLR4 mediated activation of a nuclear factor kappaB (NFkappaB) dependent reporter gene in HEK293 cells as well as LPS induced activation of NFkappaB in the astrocytoma cell line U373 and the monocytic cell line RAW264.7 ... In addition, a dominant negative form of Btk inhibited TLR4 mediated activation of a nuclear factor kappaB (NFkappaB) dependent reporter gene in HEK293 cells as well as LPS induced activation of NFkappaB in the astrocytoma cell line U373 and the monocytic cell line RAW264.7 ... In addition, a dominant negative form of Btk inhibited TLR4 mediated activation of a nuclear factor kappaB (NFkappaB) dependent reporter gene in HEK293 cells as well as LPS induced activation of NFkappaB in the astrocytoma cell line U373 and the monocytic cell line RAW264.7 ... Further investigation revealed that the Btk-specific inhibitor, LFM-A13, inhibited the activation of NFkappaB by LPS in THP-1 cells ... Further investigation revealed that the Btk-specific inhibitor, LFM-A13, inhibited the activation of NFkappaB by LPS in THP-1 cells ... Our findings implicate Btk as a Toll/interleukin-1 receptor domain binding protein that is important for NFkappaB activation by TLR4
Ahn et al., J Dermatol Sci 2003 : In order to demonstrate the possible role of NF-kappaB activation on the synthesis of melanotrophic factors from the keratinocytes, the activities of NF-kappaB induced by melanogenic inhibitors (MIs) were determined in human HaCaT keratinocytes transfected with pNF-kappaB-SEAP-NPT plasmid
Maihöfner et al., Carcinogenesis 2003 (Colorectal Neoplasms) : Finally, the study corroborates a role for NF-kappaB in the control of COX-2 gene transcription in CRC
Zerbini et al., Cancer Res 2003 (Neoplasms, Hormone-Dependent...) : Deregulated activation of the IL-6 promoter is for the most part mediated by a combined constitutive activation of the nuclear factor (NF)-kappaB p50 and p65 and the activator protein 1 (AP-1) JunD and Fra-1 family members as demonstrated by electrophoretic mobility shift assays, site directed mutagenesis, and transfection experiments
Chandrasekar et al., Biochem J 2003 : Taken together, our results demonstrate that TNF-alpha induces the expression of fractalkine and CX3CR1 in rat aortic SMCs and that this induction is mediated by NF-kappaB activation ... Taken together, our results demonstrate that TNF-alpha induces the expression of fractalkine and CX3CR1 in rat aortic SMCs and that this induction is mediated by NF-kappaB activation ... TNF-alpha activated nuclear factor kappaB (NF-kappaB) and induced fractalkine and CX3CR1 expression in a time dependent manner in rat aortic SMCs. Transient transfections with dominant negative ( dn ) inhibitory kappaB (IkappaB)-alpha, dnIkappaB-beta, dnIkappaB kinase ( IKK)-gamma, kinase-dead ( kd ) NF-kappaB inducing kinase (NIK) and kdIKK-beta, or pretreatment with wortmannin, Akt inhibitor, pyrrolidinecarbodithioc acid ammonium salt ( ` PDTC ' ) or MG-132, significantly attenuated TNF-alpha induced fractalkine and CX3CR1 expression ... TNF-alpha activated nuclear factor kappaB (NF-kappaB) and induced fractalkine and CX3CR1 expression in a time dependent manner in rat aortic SMCs. Transient transfections with dominant negative ( dn ) inhibitory kappaB (IkappaB)-alpha, dnIkappaB-beta, dnIkappaB kinase ( IKK)-gamma, kinase-dead ( kd ) NF-kappaB inducing kinase (NIK) and kdIKK-beta, or pretreatment with wortmannin, Akt inhibitor, pyrrolidinecarbodithioc acid ammonium salt ( ` PDTC ' ) or MG-132, significantly attenuated TNF-alpha induced fractalkine and CX3CR1 expression
Weinstock et al., J Immunol 2003 : Inhibition of T cell NF-kappa B activation or NF-kappa B nuclear translocation also blocked NK-1R transcription
Nelson et al., J Cell Sci 2003 : NF-kappaB signalling is inhibited by glucocorticoid receptor and STAT6 via distinct mechanisms
Kravchenko et al., J Biol Chem 2003 (Inflammation) : This link includes an NF-kappaB dependent regulation of C/EBPbeta and C/EBPdelta gene transcription and IKKi mediated activation of C/EBP ... This link includes an NF-kappaB dependent regulation of C/EBPbeta and C/EBPdelta gene transcription and IKKi mediated activation of C/EBP ... This link includes an NF-kappaB dependent regulation of C/EBPbeta and C/EBPdelta gene transcription and IKKi mediated activation of C/EBP ... Cells lacking IKKi are normal in activation of the canonical NF-kappaB pathway but fail to induce C/EBPdelta activity and transcription of C/EBP and C/EBP-NF-kappaB target genes in response to LPS
Wu et al., J Biol Chem 2003 : Overexpression of SINK inhibited NF-kappaB dependent transcription induced by tumor necrosis factor (TNF) stimulation or its downstream signaling proteins but did not inhibit NF-kappaB translocation to the nucleus and binding to DNA
Méndez-Samperio et al., Clin Diagn Lab Immunol 2003 : NF-kappaB is involved in regulation of CD40 ligand expression on Mycobacterium bovis bacillus Calmette-Guérin activated human T cells ... These data strongly suggest that CD40L expression by BCG activated CD4 ( + ) T cells is regulated via the PKC pathway and by NF-kappaB DNA binding activity
Ogasa et al., Gut 2003 : Gastrin activates nuclear factor kappaB (NFkappaB) through a protein kinase C dependent pathway involving NFkappaB inducing kinase, inhibitor kappaB (IkappaB) kinase, and tumour necrosis factor receptor associated factor 6 ( TRAF6 ) in MKN-28 cells transfected with gastrin receptor ... Gastrin induced degradation of IkappaB-alpha and activation of NFkappaB , which was abolished by the selective gastrin receptor antagonist L-740,093 and the general PKC inhibitor GF109203X ... Gastrin induced degradation of IkappaB-alpha and activation of NFkappaB , which was abolished by the selective gastrin receptor antagonist L-740,093 and the general PKC inhibitor GF109203X ... Gastrin induced phosphorylation of PKC-delta, and its inhibitor rottlerin partially suppressed NFkappaB activation ... Gastrin activates NFkappaB via a PKC dependent pathway which involves IkappaB kinase, NFkappaB inducing kinase, and TRAF6
Rodriguez-Kern et al., Neurochem Int 2003 : The BDNF dependent activation of NF-kappaB and up-regulation of GLT-1 was critically dependent on the upstream activation of p42/p44 MAP kinase signaling, whereas the inhibition of these MAP kinases dramatically increased the Abeta dependent activation of NF-kappaB and production of GLT-1 ... The BDNF dependent activation of NF-kappaB and up-regulation of GLT-1 was critically dependent on the upstream activation of p42/p44 MAP kinase signaling, whereas the inhibition of these MAP kinases dramatically increased the Abeta dependent activation of NF-kappaB and production of GLT-1 ... The BDNF dependent activation of NF-kappaB and up-regulation of GLT-1 was critically dependent on the upstream activation of p42/p44 MAP kinase signaling, whereas the inhibition of these MAP kinases dramatically increased the Abeta dependent activation of NF-kappaB and production of GLT-1
Cepinskas et al., J Cell Biol 2003 (Inflammation...) : We show that the interleukin 1beta induced accumulation of nuclear NFkappaB in human umbilical vein endothelial cell monolayers is dramatically reduced when polymorphonuclear leukocytes ( PMN ) are allowed to migrate across these monolayers ... Furthermore, cross linking of platelet-endothelial cell adhesion molecule-1 ( PECAM-1 ), but not intercellular adhesion molecule-1, reduces human umbilical vein endothelial cell nuclear NFkappaB induced by interleukin 1beta ... Furthermore, cross linking of platelet-endothelial cell adhesion molecule-1 ( PECAM-1 ), but not intercellular adhesion molecule-1 , reduces human umbilical vein endothelial cell nuclear NFkappaB induced by interleukin 1beta ... Furthermore, cross linking of platelet-endothelial cell adhesion molecule-1 ( PECAM-1 ), but not intercellular adhesion molecule-1, reduces human umbilical vein endothelial cell nuclear NFkappaB induced by interleukin 1beta ... Furthermore, cross linking of platelet-endothelial cell adhesion molecule-1 ( PECAM-1 ), but not intercellular adhesion molecule-1, reduces human umbilical vein endothelial cell nuclear NFkappaB induced by interleukin 1beta
Hah et al., Biochem Biophys Res Commun 2003 (Carcinoma, Hepatocellular...) : An absolute role of the PKC dependent NF-kappaB activation for induction of MMP-9 in hepatocellular carcinoma cells
Castro-Caldas et al., Mediators Inflamm 2003 (Leukemia-Lymphoma, Adult T-Cell) : Finally, when the cells were exposed to Dex ( 1 microM ) prior to stimulation with IL-1beta ( 20 ng/ml ), Dex was efficient in preventing IL-1beta induced NF-kappaB activation
Lee et al., Br J Pharmacol 2003 : The effects of sauchinone on the inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-alpha) and cyclooxygenase 2 (COX-2) gene expression and on the activation of transcription factors, nuclear factor-kappaB (NF-kappaB) , CCAAT/enhancer binding protein (C/EBP), activator protein-1 (AP-1) and cAMP-response element binding protein ( CREB ) were determined in Raw264.7 cells as part of the studies on its anti-inflammatory effects ... The effects of sauchinone on the inducible nitric oxide synthase (iNOS) , tumor necrosis factor-alpha (TNF-alpha) and cyclooxygenase 2 (COX-2) gene expression and on the activation of transcription factors, nuclear factor-kappaB (NF-kappaB) , CCAAT/enhancer binding protein (C/EBP), activator protein-1 (AP-1) and cAMP-response element binding protein ( CREB ) were determined in Raw264.7 cells as part of the studies on its anti-inflammatory effects ... The effects of sauchinone on the inducible nitric oxide synthase (iNOS), tumor necrosis factor-alpha (TNF-alpha) and cyclooxygenase 2 (COX-2) gene expression and on the activation of transcription factors, nuclear factor-kappaB (NF-kappaB) , CCAAT/enhancer binding protein (C/EBP), activator protein-1 (AP-1) and cAMP-response element binding protein ( CREB ) were determined in Raw264.7 cells as part of the studies on its anti-inflammatory effects
Grall et al., Arthritis Rheum 2003 (Arthritis, Rheumatoid...) : Transient transfection experiments and EMSAs showed that induction of ESE-1 gene expression by IL-1beta requires activation of NF-kappaB and binding of p50 and p65 family members to the NF-kappaB site in the ESE-1 promoter ... Transient transfection experiments and EMSAs showed that induction of ESE-1 gene expression by IL-1beta requires activation of NF-kappaB and binding of p50 and p65 family members to the NF-kappaB site in the ESE-1 promoter
Funakoshi-Tago et al., J Biol Chem 2003 : We demonstrate a critical role for FAK in the tumor necrosis factor (TNF) induced activation of nuclear factor (NF)-kappaB , using FAK-deficient ( FAK-/- ) embryonic fibroblasts ... Of note, TNF induced NF-kappaB DNA binding activity and activation of IkappaB kinases ( IKKs ) were markedly impaired in FAK-/- cells, whereas the expression of TNF receptor I or other signaling molecules such as receptor interacting protein ( RIP ), tumor necrosis factor receptor associated factor 2 ( TRAF2 ), IKKalpha, IKKbeta, and IKKgamma was unchanged
Zhu et al., J Biol Chem 2003 : Transcriptional regulation of interleukin (IL)-8 by bradykinin in human airway smooth muscle cells involves prostanoid dependent activation of AP-1 and nuclear factor (NF)-IL-6 and prostanoid independent activation of NF-kappaB ... Transcriptional regulation of interleukin (IL)-8 by bradykinin in human airway smooth muscle cells involves prostanoid dependent activation of AP-1 and nuclear factor (NF)-IL-6 and prostanoid independent activation of NF-kappaB ... Transcriptional regulation of interleukin (IL)-8 by bradykinin in human airway smooth muscle cells involves prostanoid dependent activation of AP-1 and nuclear factor (NF)-IL-6 and prostanoid independent activation of NF-kappaB ... Transcriptional activation of the IL-8 promoter by BK involves the prostanoid independent activation of NF-kappaB , and prostanoid dependent activation of AP-1 and NF-IL-6 plays a key role in augmenting the response
Takada et al., Mol Cell Biol 2003 : TNF induced activation of NF-kappaB is accelerated in SODD-deficient cells, but TNF induced c-Jun N-terminal kinase activity is slightly repressed
Takao et al., Br J Dermatol 2003 : Phorbol myristate acetate ( PMA ), tumour necrosis factor alpha, and interferon gamma each activated NF-kappaB and inhibited keratinocyte proliferation
Legler et al., Immunity 2003 : Recruitment of TNF receptor 1 to lipid rafts is essential for TNFalpha mediated NF-kappaB activation
Neu et al., Biochem Pharmacol 2003 : TNF-alpha induced apoptosis of isolated parietal cells was inhibited by pretreatment with different NF-kappaB-inhibitors , nitric oxide synthase inhibitors and with antisense-oligodeoxynucleotides against the p65 subunit of NF-kappaB
Zhang et al., Di Yi Jun Yi Da Xue Xue Bao 2003 : NF-kappa B could be activated by AGE-HSA in a time- and dose dependent manner, and the results obtained from immunochemical staining were consistent with those from EMSA ... NF-kappa B could be activated by AGE-HSA in a time- and dose dependent manner, and the results obtained from immunochemical staining were consistent with those from EMSA
Jeon et al., Exp Mol Med 2003 : Antirheumatic gold compounds have been shown to inhibit NF-kappaB activation by blocking IkappaB kinase (IKK) activity
Petro et al., Int Immunopharmacol 2003 : Expression of p35 and p40 in response to lipopolysaccharide (LPS) and interferon (IFN)-gamma requires activation of nuclear factor-kappa-B (NF-kappaB) and interferon regulatory factor ( IRF ) transcription factors ... Expression of p35 and p40 in response to lipopolysaccharide (LPS) and interferon (IFN)-gamma requires activation of nuclear factor-kappa-B (NF-kappaB) and interferon regulatory factor ( IRF ) transcription factors
Feng et al., J Biol Chem 2003 : NF-kappaB specifically activates BMP-2 gene expression in growth plate chondrocytes in vivo and in a chondrocyte cell line in vitro ... Here we have used both in vitro and in vivo approaches to demonstrate that transcription factor, NF-kappaB , regulates BMP-2 gene expression in chondrocytes ... Thus, NF-kappaB may positively regulate BMP-2 gene transcription ... Therefore, in postnatal growth plate chondrocytes, expression of BMP-2 is regulated by NF-kappaB , which may play an important role in chondrogenesis
Royle et al., J Immunol 2003 : Both purified LPS and bacterial invasion caused translocation of NF-kappa B , but whereas E5531 always inhibited activation by purified LPS, activation by bacterial invasion was only inhibited at later time points
Strengell et al., J Immunol 2003 : IL-18 , however, activated the binding of NF-kappa B to the IFN-gamma promoter NF-kappa B site
Abraham et al., J Immunol 2003 (Acute Disease) : In particular, uPA increased LPS induced activation of intracellular signaling pathways, including Akt and c-Jun N-terminal kinase, nuclear translocation of the transcriptional regulatory factor NF-kappa B , and expression of proinflammatory cytokines, including IL-1 beta, macrophage-inflammatory protein-2, and TNF-alpha ... In particular, uPA increased LPS induced activation of intracellular signaling pathways, including Akt and c-Jun N-terminal kinase, nuclear translocation of the transcriptional regulatory factor NF-kappa B , and expression of proinflammatory cytokines, including IL-1 beta, macrophage-inflammatory protein-2, and TNF-alpha
Chae et al., Res Commun Mol Pathol Pharmacol 2001 : However, a specific inhibitor of p38 MAPK , SB203580, had no effect on TNF-alpha/IFN-gamma or LPS/IFN-gamma induced NF-kappaB activation ... However, a specific inhibitor of p38 MAPK, SB203580, had no effect on TNF-alpha/IFN-gamma or LPS/IFN-gamma induced NF-kappaB activation ... However, a specific inhibitor of p38 MAPK, SB203580, had no effect on TNF-alpha/IFN-gamma or LPS/IFN-gamma induced NF-kappaB activation
Bamba et al., Am J Physiol Gastrointest Liver Physiol 2003 : IL-11 inhibits the activation of NF-kappaB and induces the Th2 polarization of CD4+ T cells
Pingle et al., Mol Pharmacol 2003 : Both of these agents also reduced the activation of NF-kappaB induced by LPS, tumor necrosis factor-alpha and interleukin-1beta in smooth muscle cells ... Both of these agents also reduced the activation of NF-kappaB induced by LPS, tumor necrosis factor-alpha and interleukin-1beta in smooth muscle cells ... Both of these agents also reduced the activation of NF-kappaB induced by LPS , tumor necrosis factor-alpha and interleukin-1beta in smooth muscle cells
Kimura et al., Cell Death Differ 2003 (Carcinoma...) : Immunostaining and EMSA revealed that NF-kappaB was activated strongly by TNF/IFN-alpha compared to TNF alone in a human colon adenocarcinoma cell line, RPMI4788 ... On the other hand, Fas expression was strongly enhanced by TNF/IFN-alpha, and inhibition of TNF/IFN-alpha induced NF-kappaB activation, by using NF-kappaB decoy, decreased Fas expression
Escotte et al., Eur Respir J 2003 (Cystic Fibrosis) : To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-kappaB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-kappaB dependent interleukin (IL)-6 , IL-8 and RANTES ( regulated on activation, T-cell expressed and secreted ) along with the activation of NF-kappaB in non-CF and CF human bronchial gland epithelial cells ... To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-kappaB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-kappaB dependent interleukin (IL)-6, IL-8 and RANTES ( regulated on activation, T-cell expressed and secreted ) along with the activation of NF-kappaB in non-CF and CF human bronchial gland epithelial cells
Choi et al., Blood 2003 : LPS, but not GM-CSF or DEX, activated NF-kappaB as shown by IkappaBalpha degradation, NF-kappaB DNA binding, and transcriptional activity ... LPS , but not GM-CSF or DEX, activated NF-kappaB as shown by IkappaBalpha degradation, NF-kappaB DNA binding, and transcriptional activity ... The TAT-NBD blocked LPS induced NF-kappaB activation and NF-kappaB dependent gene expression ... The TAT-NBD blocked LPS induced NF-kappaB activation and NF-kappaB dependent gene expression
Ediger et al., Eur Respir J 2003 (Cell Transformation, Neoplastic...) : In contrast, nuclear factor (NF)-kappaB was activated by LPA but not EGF and NF-kappaB activation was completely blocked only when Rho was inhibited ... In contrast, nuclear factor (NF)-kappaB was activated by LPA but not EGF and NF-kappaB activation was completely blocked only when Rho was inhibited
Németh et al., J Pharmacol Exp Ther 2003 : Furthermore, adenosine as well as adenosine receptor agonists failed to decrease LPS induced NF-kappaB DNA binding, NF-kappaB promoter activity, p65 nuclear translocation, and inhibitory kappaB degradation
Fu et al., Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai) 2003 : Our results showed that the two mutants induced significantly less amount of IFN-gamma from PBMC ( 13 %, 48 % of wild type rhIL-18 for Delta NC, S, respectively ), and the activation of NF-kappa B also lower than wild type rhIL-18 ( 69.7 %, 89.8 % of wild type rhIL-18 respectively ), indicating that the deleted or mutated amino acids might be important for IL-18 function
Murakami et al., Cancer Lett 2003 : Degradation of IkappaB ( IkappaB ) is a key step for nuclear factor-kappaB (NF-kappaB) induced transcription of certain proinflammatory genes, including inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 ... Degradation of IkappaB ( IkappaB ) is a key step for nuclear factor-kappaB (NF-kappaB) induced transcription of certain proinflammatory genes, including inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 ... Degradation of IkappaB ( IkappaB ) is a key step for nuclear factor-kappaB (NF-kappaB) induced transcription of certain proinflammatory genes, including inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2
Han et al., Biochem Biophys Res Commun 2003 : TNF related weak inducer of apoptosis receptor, a TNF receptor superfamily member, activates NF-kappa B through TNF receptor associated factors ... In this report we identify the TNF receptor associated factor (TRAF) family of signal transducers as important components of TweakR mediated NF-kappa B activation ... Dominant negative forms of TRAF2 and TRAF5 substantially inhibited TweakR mediated NF-kappa B activation, suggesting a role of TRAFs in regulating smooth muscle and endothelial cell function ... Dominant negative forms of TRAF2 and TRAF5 substantially inhibited TweakR mediated NF-kappa B activation, suggesting a role of TRAFs in regulating smooth muscle and endothelial cell function ... Dominant negative forms of TRAF2 and TRAF5 substantially inhibited TweakR mediated NF-kappa B activation, suggesting a role of TRAFs in regulating smooth muscle and endothelial cell function
Beppu et al., Biochem Biophys Res Commun 2003 (Carcinoma...) : These findings suggest the existence of IFN-gamma induced anti-apoptotic pathway and we evaluated the effect of IFN-gamma and CsA on calcium-sensitive nuclear factor-kappa B (NF-kappa B) activation ... Activation of NF-kappa B occurred in response to IFN-gamma , and which was markedly inhibited by either CsA or FK506 ... These results suggest that IFN-gamma may simultaneously induce the STAT1 mediated apoptotic pathway and the anti-apoptotic pathway through calcium activated NF-kappa B and that inhibition of the latter by CsA may result in dominance of the apoptosis inducing pathway
Vallée et al., Biochem Biophys Res Commun 2003 (Adenocarcinoma...) : Indeed, TNF alpha induced NF-kappa B translocation was not sufficient to support enhancement of the transcription and des-IGF-1 did not promote but partly inhibited both the TNF alpha induced NF-kappa B activation and I kappa B alpha degradation through a PI-3K dependent pathway ... Indeed, TNF alpha induced NF-kappa B translocation was not sufficient to support enhancement of the transcription and des-IGF-1 did not promote but partly inhibited both the TNF alpha induced NF-kappa B activation and I kappa B alpha degradation through a PI-3K dependent pathway
Bae et al., Biochem Biophys Res Commun 2003 : S535A mutant of p65 was specifically defective in transactivation of NF-kappa B target gene expression induced by CaMKIV
Wu et al., Zhonghua Er Bi Yan Hou Ke Za Zhi 2002 : The radiolabeled probe bound differently to nuclear extracts from the LPS treated and the saline treated mouse cochleae, showing that LPS inoculation significantly increased NF-kappa B binding activity ... LPS could induce NF-kappa B activation in the cochlea
Lee et al., Life Sci 2003 : The regulation of inducible nitric oxide synthase gene expression induced by lipopolysaccharide and tumor necrosis factor-alpha in C6 cells : involvement of AP-1 and NFkappaB ... The roles of AP-1 and NFkappaB in the regulation of inducible nitric oxide synthase (iNOS) mRNA expression induced by the combination of lipopolysaccharide and tumor necrosis factor-alpha ( LT ) in C6 cells were examined in the present study
Das et al., J Biol Chem 2003 (Breast Neoplasms) : However, the molecular mechanism by which upstream kinases regulate the OPN induced NFkappaB activation and urokinase type plasminogen activator ( uPA ) secretion in human breast cancer cells is not well defined ... OPN also induces NFkappaB activation through phosphorylation and degradation of IkappaBalpha by inducing the IKK activity ... OPN also induces NFkappaB activation through phosphorylation and degradation of IkappaBalpha by inducing the IKK activity ... However, both pharmacological ( wortmannin and LY294002 ) and genetic ( Deltap85 ) inhibitors of PI 3'-kinase inhibited OPN induced Akt phosphorylation, IKK activity, and NFkappaB activation through phosphorylation and degradation of IkappaBalpha ... However, both pharmacological ( wortmannin and LY294002 ) and genetic ( Deltap85 ) inhibitors of PI 3'-kinase inhibited OPN induced Akt phosphorylation, IKK activity, and NFkappaB activation through phosphorylation and degradation of IkappaBalpha ... To our knowledge, this is first report that OPN induces NFkappaB activity and uPA secretion by activating PI 3'-kinase/Akt/IKK mediated signaling pathways and further demonstrates a functional molecular link between OPN induced PI 3'-kinase dependent Akt phosphorylation and NFkappaB mediated uPA secretion, and all of these ultimately control the motility of breast cancer cells ... To our knowledge, this is first report that OPN induces NFkappaB activity and uPA secretion by activating PI 3'-kinase/Akt/IKK mediated signaling pathways and further demonstrates a functional molecular link between OPN induced PI 3'-kinase dependent Akt phosphorylation and NFkappaB mediated uPA secretion, and all of these ultimately control the motility of breast cancer cells
Li et al., FASEB J 2003 : The UbcH2 promoter region contains a functional NF-kappaB binding site ; NF-kappaB binding to this sequence is increased by TNF-alpha stimulation ... A dominant negative inhibitor of NF-kappaB activation blocks both UbcH2 up-regulation and the increase in ubiquitin conjugating activity stimulated by TNF-alpha
Lee et al., Int J Hematol 2003 (Hodgkin Disease) : In HL pathogenesis associated with Epstein-Barr virus infection, the activation of NF-kappaB is induced by viral latent membrane protein 1 (LMP1)
Vital et al., Mediators Inflamm 2003 : GM-CSF induced iNOS expression and NO production, and activated the transcription factor NF-kappaB ... Dexamethasone also inhibited GM-CSF induced NF-kappaB activation by preventing a significant decrease on the IkappaB-alpha protein levels, thus blocking NF-kappaB migration to the nucleus ... The corticosteroid dexamethasone inhibits GM-CSF induced NF-kappaB activation, iNOS protein expression and NO production
Stehlik et al., J Biol Chem 2003 : CARD6 is a modulator of NF-kappa B activation by Nod1- and Cardiak mediated pathways ... In transfection experiments, the CARD6 protein suppressed NF-kappa B induction by Nod1 or Cardiak but did not interfere with NF-kappa B activation by the CARD containing adapter protein Bcl10 or the cytokine tumor necrosis factor-alpha, demonstrating specificity of CARD6 for Nod-1 and Cardiak dependent pathways ... In transfection experiments, the CARD6 protein suppressed NF-kappa B induction by Nod1 or Cardiak but did not interfere with NF-kappa B activation by the CARD containing adapter protein Bcl10 or the cytokine tumor necrosis factor-alpha, demonstrating specificity of CARD6 for Nod-1 and Cardiak dependent pathways ... Thus, CARD6 represents a selective modulator of NF-kappa B activation by Cardiak and Nod1 , adding to the repertoire of CARD-family proteins implicated in inflammatory responses and innate immunity
Park et al., J Biol Chem 2003 : In HEK293 cells, where tumor necrosis factor-alpha can activate NF-kappa B , SNP likewise suppresses the binding of the active NF-kappa B complex, restoring the binding of the repressive p50/p50 homodimer complex ... In light of findings that NF-kappa B stimulates Myc oncoprotein expression in cancers, our findings suggest that NO should be investigated as a prospective therapeutic cancer agent
Saura et al., J Neurochem 2003 : LPS robustly increased NF-kappa B activation in mixed glial cultures ... Two NF-kappa B inhibitors, aspirin ( 10 mM ) and MG-132 ( 0.1 microM ), blocked basal apoE and apoJ secretion as well as LPS induced apoJ secretion ... Two NF-kappa B inhibitors, aspirin ( 10 mM ) and MG-132 ( 0.1 microM ), blocked basal apoE and apoJ secretion as well as LPS induced apoJ secretion
Anest et al., Nature 2003 : NF-kappaB is a principal transcriptional regulator of diverse cytokine mediated processes and is tightly controlled by the IkappaB kinase complex ( IKK-alpha/beta/gamma )
Yoneshima et al., Int J Antimicrob Agents 2003 : We tested the hypothesis that fosfomycin inhibits the activation of NF-kappaB induced by tumor necrosis factor-alpha (TNF-alpha) in human monocytic U-937 cells, and a T cell line ( Jurkat )
Akira et al., J Infect Dis 2003 : After stimulation, TLR recruits IL-1R associated kinase via adaptor myeloid differentiation factor 88 ( MyD88 ) and induces activation of NF-kappaB and mitogen activated protein kinases
Pullerits et al., Arthritis Rheum 2003 (Arthritis, Experimental...) : In addition, spleen cells were cultured in the presence of HMGB-1 , and nuclear factor kappaB (NF-kappaB) activation was detected by electrophoretic mobility shift assay
Gately et al., Prog Exp Tumor Res 2003 (Neoplasms...) : Some of the COX independent mechanisms for NSAIDs and selective COX-2 inhibitors include activation of protein kinase G, inhibition of NF-kappa B activation, downregulation of the antiapoptotic protein Bcl-XL, inhibition of PPAR delta, and activation of PPAR gamma
Park et al., J Biol Chem 2003 (Hepatitis C) : Both TNF-alpha- and TRAF2 mediated nuclear factor-kappaB (NF-kappaB) activations were inhibited by NS5A-TRAF2 interaction ... Because TRAF2 is required for the activation of both NF-kappaB and c-Jun N-terminal kinase (JNK), we investigated HCV NS5A protein for its potential capacity to modulate TRAF2 mediated JNK activity
Schmeck et al., Inflammation 2003 : Therefore, RhoA/Rac1/Cdc42 contribute to NF-kappaB dependent LPS- and TNFalpha induced expression of PGHS-2 in EC but had no effect on the activity of expressed COX-1 and COX-2 ... Therefore, RhoA/Rac1/Cdc42 contribute to NF-kappaB dependent LPS- and TNFalpha induced expression of PGHS-2 in EC but had no effect on the activity of expressed COX-1 and COX-2
O'Sullivan et al., Curr Opin Hematol 2003 : NFkappaB is involved both in the regulation of CD40 expression and in cell signaling after CD40 ligation
Ichiyama et al., Clin Exp Allergy 2003 : We examined whether or not pranlukast inhibits TNF-alpha induced activation of nuclear transcription factor NF-kappa B , a factor that is essential for the expression of proinflammatory cytokines, on human monocytic 1.3 % dimethylsulphoxide ( DMSO ) -differentiated U-937 cells, which have cysteinyl LT1 ( CysLT1 ) receptors on their membranes, and T cells ( Jurkat ), which do not ... The inhibitory effects of pranlukast and MK-571, which is an LTD4 receptor-selective antagonist, on TNF-alpha induced NF-kappa B activation was evaluated by Western blotting and flow cytometry, and those on lipopolysaccharide (LPS) induced interleukin-6 (IL-6) production in peripheral blood mononuclear cells ( PBMC ) were evaluated by enzyme linked immunosorbent assaying
Kurland et al., J Biol Chem 2003 (Lymphoma, B-Cell) : In a previously published report ( Kurland, J. F., Kodym, R., Story, M. D., Spurgers, K. B., McDonnell, T. J., and Meyn, R. E. ( 2001 ) J. Biol. Chem. 276, 45380-45386 ), we described the NF kappa B status for two murine B-cell lymphoma cell lines, LY-as ( apoptosis-sensitive ) and LY-ar ( apoptosis-refractory ) and provided evidence that NF kappa B1 ( p50 ) homodimers contribute to the expression of Bcl-2 in the LY-ar line
Milani et al., J Neurochem 2003 (Neuroblastoma) : TRAIL induced distinct short-term ( 1-60 min ) and long-term ( 3-24 h ) effects on the protein kinase B (PKB)/Akt ( Akt ), extracellular signal regulated kinase ( ERK ), cAMP response element binding protein ( CREB ), nuclear factor kappa B (NF-kappaB) and caspase pathways
Shishodia et al., Carcinogenesis 2003 (Carcinoma, Non-Small-Cell Lung...) : The inhibition of NF-kappaB activation correlated with suppression of CS-induced NF-kappaB dependent cyclin D1 , cyclooxygenase-2 and matrix metalloproteinase-9 expression ... The inhibition of NF-kappaB activation correlated with suppression of CS-induced NF-kappaB dependent cyclin D1, cyclooxygenase-2 and matrix metalloproteinase-9 expression
Shimada et al., Carcinogenesis 2003 (Prostatic Neoplasms) : Nuclear factor kappaB (NFkappaB) was activated by 2-ME and closely regulated by the mitogen activated protein kinase, p38 ... Inhibition of p38 or NFkappaB resulted in suppression of p53 induction and apoptosis ... The results suggest that not only p53 induction through p38/JNK dependent NFkappaB/AP-1 activation but also JNK dependent Bcl-2 phosphorylation are required for 2-ME induced apoptosis ; moreover, inhibition of these pathways may be involved in androgen mediated resistance to apoptosis
Wroblewski et al., J Cell Sci 2003 (Neoplasm Invasiveness) : RhoA acted through activation of both NF-kappaB and AP-1, whereas Rac activated NF-kappaB but not AP-1 ... RhoA acted through activation of both NF-kappaB and AP-1, whereas Rac activated NF-kappaB but not AP-1
Sanchez et al., Mol Cell Biol 2003 : In light of reports on the interplay between GSK-3beta and nuclear factor kappaB (NF-kappaB), and because of the antiapoptotic activity of NF-kappaB, we examined the effect of GSK-3beta overexpression on NF-kappaB activation
Rocha et al., Mol Cell Biol 2003 : p53 represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-kappaB subunit with histone deacetylase 1 ... p53 represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-kappaB subunit with histone deacetylase 1 ... p53 represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-kappaB subunit with histone deacetylase 1 ... p53 represses cyclin D1 transcription through down regulation of Bcl-3 and inducing increased association of the p52 NF-kappaB subunit with histone deacetylase 1
Cao et al., Proc Natl Acad Sci U S A 2003 : The amino-terminal portion of E1A decreases transactivation of the NOS2 5'-flanking region, limiting the DNA binding activity of NF-kappaB and inhibiting NOS2 expression
Woo et al., Cancer Res 2003 (Neoplasm Invasiveness) : These data demonstrate a role for NF kappa B in the regulation of MMP-9 expression and the ability of dykellic acid to suppress this action of NF kappa B
Sivakumar et al., J Exp Med 2003 : Moreover, NF-kappa B-inducing kinase (NIK) was required for both constitutive thymic DNA binding of RelB and the specific induction of RelB complexes in vitro
Johannessen et al., J Gen Virol 2003 : Mutation of the TATA box had no effect on TNFalpha- or RelA/p65 mediated induction of NFkappaB-responsive promoters, indicating a specific st-ag effect on hTAF ( II ) 130/135 ... Mutation of the TATA box had no effect on TNFalpha- or RelA/p65 mediated induction of NFkappaB-responsive promoters, indicating a specific st-ag effect on hTAF ( II ) 130/135 ... Mutation of the TATA box had no effect on TNFalpha- or RelA/p65 mediated induction of NFkappaB-responsive promoters, indicating a specific st-ag effect on hTAF ( II ) 130/135
Neish et al., J Exp Med 1992 : NF-kappa B-mediated activation of VCAM1 gene expression may lead to endothelial expression of a mononuclear leukocyte adhesion molecule associated with initial events in the development of an atherosclerotic lesion
Malewicz et al., J Biol Chem 2003 : In addition, NF kappa B downstream of TNFR was required for up-regulation of Fas expression by endogenous TNF secreted in response to TCR stimulation ... In addition, NF kappa B downstream of TNFR was required for up-regulation of Fas expression by endogenous TNF secreted in response to TCR stimulation
Kishore et al., J Biol Chem 2003 (Inflammation) : A selective IKK-2 inhibitor blocks NF-kappa B-dependent gene expression in interleukin-1 beta stimulated synovial fibroblasts
Ehrhardt et al., Oncogene 2003 (Acute Disease...) : Our data describe the biological significance of TRAIL mediated activation of NF-kappaB in cancer cells resistant to TRAIL mediated apoptosis : TRAIL leads to an increase in tumor cell count by a prosurvival and possibly mitogenic function
Banan et al., J Pharmacol Exp Ther 2003 : We found the following : 1 ) Oxidants caused I-kappaBalpha degradation, NF-kappaB translocation, NF-kappaB ( p50 and p65 subunits ) activation , tubulin disassembly ( upward arrow S1, downward arrow S2 ), microtubule architectural instability, and barrier disruption ... 2 ) EGF ( 10 ng/ml ) stabilized I-kappaBalpha and prevented both NF-kappaB translocation and activation while protecting monolayers against oxidants
Debierre-Grockiego et al., J Biol Chem 2003 : Stimulation of macrophages with GPIs of T. gondii results in activation of the transcription factor NF-kappa B, which is inhibited by the chemically synthesized GPIb, suggesting the involvement of NF-kappa B in TNF alpha gene expression
Liang et al., Mol Pharmacol 2003 : Namely, JD inhibits tumor necrosis factor-alpha induced activation of NF-kappaB in mouse 3T3 and human HeLa cells
Yoon et al., J Biol Chem 2003 : We found that pretreatment of cells with alpha-MSH blocked LPS induced p38 kinase and NF kappa B activation as well as TNF-alpha production
Khapli et al., J Immunol 2003 : IL-3 acts directly on osteoclast precursors and irreversibly inhibits receptor activator of NF-kappa B ligand induced osteoclast differentiation by diverting the cells to macrophage lineage ... In osteoclast precursors, IL-3 prevents RANKL induced nuclear translocation of NF-kappaB by inhibiting the phosphorylation and degradation of IkappaB ... In osteoclast precursors, IL-3 prevents RANKL induced nuclear translocation of NF-kappaB by inhibiting the phosphorylation and degradation of IkappaB
Fernandez et al., Immunity 2003 (Inflammation) : Specific dIgA colocalizes to lipopolysaccharide (LPS) in the apical recycling endosome compartment, preventing LPS induced NF-kappaB translocation and subsequent proinflammatory response
Massaad et al., Neuroreport 2003 : Bcl-2 phosphorylation is not required for its effects on NFkappaB activity ... To study whether effects on NFkappaB are part of the Bcl-2 anti-apoptotic mechanism, we examined the effect of Bcl-2 on NFkappaB transcriptional activity in PC12 cells and determined the role thereby of Bcl-2 phosphorylation ( required for the anti-apoptotic function of Bcl-2 ) ... We conclude that such Bcl-2 effect on NFkappaB activity is distinct from its anti-apoptotic action
Hu et al., Cytokine 2003 : Activation of NF-kappaB is dependent on the degradation of IkappaBalpha
Castro et al., Bioorg Med Chem Lett 2003 : In addition, we show that one of these beta-carboline analogues inhibits the phosphorylation of IkappaBalpha and subsequent activation of NF-kappaB in whole cells, as well as blocking TNF-alpha release in LPS challenged mice
Huang et al., J Biomed Sci 2003 : We found that LPS induced I kappa B kinase and nuclear factor-kappa B (NF-kappa B) activation , as well as IFN-gamma induced signal transducer and activator of transcription 1 ( STAT1 ) phosphorylation, were reduced by lovastatin ... We found that LPS induced I kappa B kinase and nuclear factor-kappa B (NF-kappa B) activation, as well as IFN-gamma induced signal transducer and activator of transcription 1 ( STAT1 ) phosphorylation, were reduced by lovastatin
Dobashi et al., Free Radic Biol Med 2003 : These results suggest that cAMP regulates iNOS expression in adipocytes through modulating NF-kappaB activity
Hashimoto et al., Pancreas 2003 (Acute Disease...) : CONCLUSION Our findings demonstrate that 15d-PGJ2 attenuates the severity of AP most likely through the inhibition of COX-2 expression, IL-6 production, and NF-kappaB activation
Kirchner et al., Exp Hematol 2003 (Leukemia, Myelogenous, Chronic, BCR-ABL Positive) : Mechanisms of Bcr-Abl mediated NF-kappaB/Rel activation ... Mechanisms of Bcr-Abl mediated NF-kappaB/Rel activation ... In this paper we demonstrate activation of NF-kappaB/Rel by Bcr-Abl and for the first time by v-Abl ... In this paper we demonstrate activation of NF-kappaB/Rel by Bcr-Abl and for the first time by v-Abl ... Furthermore, we investigated mechanisms of NF-kappaB/Rel induction by Bcr-Abl and v-Abl ... Furthermore, we investigated mechanisms of NF-kappaB/Rel induction by Bcr-Abl and v-Abl
Park et al., J Biol Chem 2003 : Heat shock protein 27 association with the I kappa B kinase complex regulates tumor necrosis factor alpha induced NF-kappa B activation
Muta et al., J Endotoxin Res 2003 : NF-kappaB activity is essential for the induction of IkappaB-zeta , but is not sufficient
Akhtar et al., FASEB J 2003 (MAP Kinase Signaling System) : In contrast, inhibitors of NFkappaB activity ( PDTC, SN50 ) did not block E. coli DNA induced IL-8 production
Yamamoto et al., Cell Signal 2003 : Moreover, the transfection of CD59 antisense oligonucleotide into keratinocytes markedly suppressed LPS induced nuclear translocation of NF-kappaB and cytokine generation
Viatour et al., Leukemia 2003 (Breast Neoplasms...) : NF- kappa B2/p100 induces Bcl-2 expression
Iwanaga et al., Inflamm Res 2003 (Arthritis...) : Expression of mouse NOD2 into HEK cells resulted in NF-kappaB activation
Hou et al., J Neurosci Res 2003 : The inhibitors of PKC, JNK MAPK and NF-kappaB , but not p38 or ERK1/2 MAPK, blocked IL-1beta induced CGRP release
Xu et al., Zhonghua Zhong Liu Za Zhi 2003 (Leukemia P388) : The activation of NF-kappa B induced by Ara-C and Vp-16 was obviously correlated to apoptosis in P388 cells
Liu et al., Am J Physiol Cell Physiol 2003 : These data indicate that MHb is a potent activator of endothelial cells through NF-kappaB mediated upregulation of cell adhesion molecule expression and chemokine and cytokine production
MacKenzie et al., Br J Pharmacol 2003 (MAP Kinase Signaling System) : 1. In rat aortic smooth muscle cells ( RASMCs ), the putative nuclear factor kappa B (NFkappaB) inhibitor Pyrrolidine dithiocarbamate ( PDTC ) was found to inhibit lipopolysaccharide (LPS) stimulated NFkappaB DNA binding ... 3. Another inhibitor of NFkappaB signalling, the serine protease inhibitor Nalphap-tosyl-L-lysine chloro-methylketone ( TLCK ), also inhibited LPS stimulated IKK activity and potentiated JNK activity in response to LPS, suggesting that cross-talk may occur between the NFkappaB and stress activated protein kinase pathways at the level of IKK or at a common point upstream
Levine et al., Cancer Res 2003 (Neovascularization, Pathologic...) : We show that BBS treatment induced inhibitor of NF kappa B degradation, NF kappa B translocation to the cell nucleus, increased NF kappa B binding to its DNA consensus sequence, and increased IL-8 and VEGF mRNA expression and protein secretion ... Treatment with the proteasome inhibitor, MG-132, blocked BBS stimulated NF kappa B DNA binding, and IL-8 and VEGF expression and secretion
Liu et al., Cancer Res 2003 (Colonic Neoplasms) : Electrophoretic mobility shift assay on nuclear extracts demonstrated that IL-1 beta induced NF-kappa B DNA binding activity in HT-29 cells, and the activated NF-kappa B complex was eliminated after treatment with an inhibitor of NF-kappa B ... Supershift assay indicated that the two NF-kappa B subunits, p65 and p50, were involved in activation of NF-kappa B complex by IL-1 beta stimulation
Hayakawa et al., EMBO J 2003 : This hypothesis is mainly based on the findings that N-acetyl-L-cysteine (NAC) and pyrrolidine dithiocarbamate ( PDTC ), compounds recognized as potential antioxidants, can inhibit NF-kappaB activation in a wide variety of cell types ... Here we reveal that both NAC and PDTC inhibit NF-kappaB activation independently of antioxidative function
Saitoh et al., J Biol Chem 2003 : TWEAK induces NF-kappaB2 p100 processing and long lasting NF-kappaB activation ... Unlike TNF-alpha, TWEAK stimulation results in prolonged NF-kappaB activation with a transition of the DNA binding NF-kappaB components from RelA- to RelB containing complexes by 8 h, and the latter remained active in binding at least until 24 h post-stimulation
Hugot et al., Eur J Gastroenterol Hepatol 2003 (Crohn Disease...) : Because CARD15 is able to be activated by components of the bacterial wall and further induce the activation of NFkappaB , a proinflammatory molecule, CARD15 discovery makes the link, at the molecular level, between bacteria and inflammation of the digestive tract
Ouellet et al., Biochemistry 2003 : NF-kappaB induction by bisperoxovanadium compounds requires CD45, p36 ( LAT ), PKC, and IKK activity and exhibits kinetics of activation comparable to those of TCR/CD28 coengagement ... NF-kappaB induction by bisperoxovanadium compounds requires CD45, p36 ( LAT ), PKC, and IKK activity and exhibits kinetics of activation comparable to those of TCR/CD28 coengagement ... NF-kappaB induction by bisperoxovanadium compounds requires CD45 , p36 ( LAT ), PKC, and IKK activity and exhibits kinetics of activation comparable to those of TCR/CD28 coengagement
Cho et al., Toxicol Lett 2003 : The dominant negative mutant forms of TAK1 and NIK inhibited the silica induced NF-kappaB activation in Rat2 cells
Huang et al., Cytokine 2003 : LPS increased NF-kappaB nuclear translocation in the presence or absence of MS
Hanson et al., J Biol Chem 2003 : Oncoprotein suppression of tumor necrosis factor induced NF kappa B activation is independent of Raf controlled pathways ... Consistent with this, evidence has been presented that NF kappa B activity is required for Ras and BCR-ABL to transform cells ... Consistent with this, evidence has been presented that NF kappa B activity is required for Ras and BCR-ABL to transform cells ... The data presented here indicate that BCR-ABL expression in 32D myeloid cells or oncogenic Ras expression in murine fibroblasts blocks the ability of tumor necrosis factor (TNF) to activate NF kappa B ... The data presented here indicate that BCR-ABL expression in 32D myeloid cells or oncogenic Ras expression in murine fibroblasts blocks the ability of tumor necrosis factor (TNF) to activate NF kappa B ... This suppression of NF kappa B is manifested by an inhibition of TNF induced inhibitor of NF kappa B ( IKK ) activity and NF kappa B DNA binding potential but not by blocking TNF induced nuclear accumulation of NF kappa B/p65 ... The inhibition of NF kappa B is not observed in oncogenic Raf expressing cells and is not fully restored by the suppression of PI3-kinase or MEK pathways
Matsumura et al., Cell cycle (Georgetown, Tex.) 2003 : c-Myc and E2F1 inhibit NF-kappaB activities induced by TNFalpha or reactive oxygen species ... c-Myc and E2F1 inhibit NF-kappaB activities induced by TNFalpha or reactive oxygen species ... c-Myc and E2F1 inhibit NF-kappaB activities induced by TNFalpha or reactive oxygen species
Jiang et al., Ann N Y Acad Sci 2003 : Both NMDA and TrkB receptors activate nuclear factor kappaB (NF-kappaB) , a transcription factor known to be involved in protecting neurons against many different kinds of toxic insults
Chen et al., J Urol 2003 (Carcinoma, Transitional Cell...) : We tested the hypothesis that BCG initiates NF-kappa B signaling as a consequence of cross linking alpha 5 beta 1 integrin receptors present on the tumor cell surface
Park et al., Oncogene 2003 (Prostatic Neoplasms) : IL-6 activation by TGF-beta1 was accompanied by nuclear translocation of NF-kappaB , which was blocked by the p38 inhibitors SB202190 and SB203580 or by IkappaBalphaDeltaN transfection, indicating the crucial role for the p38-NF-kappaB signaling in TGF-beta1 induction of IL-6
Chen et al., Oncogene 2003 : Gene knockout study, however, suggests that NIK is dispensable for TNF-R1- but required for lymphotoxin-beta receptor induced NF-kappaB activation ... In reporter gene assays, NIK could potentiate Grb7, ErbB2/ErbB4, and EGF induced NF-kappaB activation ... A NIK kinase inactive mutant could block ErbB2/ErbB4 and EGF induced NF-kappaB activation ... Moreover, EGF/heregulin receptors activated NF-kappaB in wild-type, but not NIK-/- embryonic fibroblasts
Wuyts et al., Respir Med 2003 (Asthma...) : We conclude that p38 MAPK, JNK kinase, ERK and NF-kappaB are involved in the IL-1beta induced eotaxin, MCP-1 , and MCP-3 expression and release in HASMC ... We conclude that p38 MAPK, JNK kinase, ERK and NF-kappaB are involved in the IL-1beta induced eotaxin, MCP-1, and MCP-3 expression and release in HASMC
Walsh et al., Am J Respir Cell Mol Biol 2004 (Neuroblastoma) : Inhibition of ERK1/2 prevented NF-kappaB activation
Karashima et al., Clin Cancer Res 2003 (Anoxia...) : Nuclear factor kappaB (NF-kappaB)/relA regulates IL-8 expression in several neoplasms ... The purpose of this study was to determine whether the organ microenvironment ( hypoxia, acidosis ) regulates the expression of IL-8 in TCC via NF-kappaB, and whether inhibition of NF-kappaB function by mutant IkappaB-alpha prevents induction of IL-8 expression ... The involvement of NF-kappaB and activator protein 1 in the regulation of IL-8 production was evaluated by electrophoretic mobility shift assay
Nguyen et al., J Interferon Cytokine Res 2003 : Chemical inhibitors or dominant negative forms of signaling components required to activate NF-kappa B , ATF, or AP-1 in response to IL-1 do not affect the phosphorylation of Stat1 on serine
Gan et al., Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2003 (Colitis, Ulcerative) : The results showed that NF-kappa B p65 antisense oligonucleotides resulted in down-regulation of NF-kappa B p65 expression, blocked the expression of IL-1 beta mRNA and IL-8 mRNA, and strikingly reduced the production of IL-1 beta and IL-8, and these effects were greater than those of dexamethasone in cultured LPMC from patients with UC ( P < 0.05 )
Yin et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2003 : In the LPS stimulated group, the expression of IKK-beta mRNA ( 0.147+/-0.015 ) and activity of NF-kappaB ( 0.828+/-0.019 ) in PAM significantly increased , whereas levels of IkappaB-alpha ( 0.228+/-0.021 ) decreased ( all P < 0.01 ) in PMB treated groups ... LPS might induce expression of IKK-beta mRNA, degradation of IkappaB-alpha and activation of NF-kappaB PMB could inhibit the expression of IKK-beta, degradation of IkappaB-alpha and activation of NF-kappaB , showing marked anti-inflammatory property
Dudziak et al., J Virol 2003 : The inducibility of the CD83 promoter by LMP1 was mediated by the activation of NF-kappaB , as seen by use of luciferase reporter assays using the CD83 promoter and LMP1 mutants
Shin et al., Exp Mol Med 2003 : These results suggest that activations of NF-kappaB were involved in induction of iNOS by GITR and IFN-gamma priming caused earlier and stronger NF-kappaB activation
Joo et al., Biochem Biophys Res Commun 2003 (Hepatoblastoma...) : Involvement of NF-kappaB in the regulation of S100A6 gene expression in human hepatoblastoma cell line HepG2 ... Transient expression of NF-kappaB ( p65 ) increased S100A6 promoter activity and expression of inhibitor of NF-kappaB ( IkappaBalpha ) decreased TNFalpha induced S100A6 promoter activity ... To confirm the involvement of NF-kappaB in S100A6 promoter activation, we analyzed serially deleted promoter constructs of the S100A6 gene by luciferase reporter assay and found a NF-kappaB-responsive DNA fragment at the position between -584 and -361
Wu et al., Leuk Res 2003 (Blast Crisis...) : Since nuclear factor-kappaB (NF-kappaB) is activated by TNFalpha and induces expression of prosurvival genes, effects of the antisense oligodeoxynucleotides to NF-kappaB subunits, p65 and p50, were examined
Kim et al., Cancer Lett 2003 : Transient expression and electrophoretic mobility shift assays with NF-kappaB binding sites revealed that BPA reduced the levels of the LPS induced NF-kappaB transcription factor complex ... Transient expression and electrophoretic mobility shift assays with NF-kappaB binding sites revealed that BPA reduced the levels of the LPS induced NF-kappaB transcription factor complex
Tozawa et al., Cancer Lett 2003 (Neoplasms) : Here we demonstrate that IL-1beta induces nuclear translocation of NF-kappaB in human umbilical vein endothelial cells ( HUVEC ) followed by induction of cell surface expression of E-selectin, ICAM-1 and VCAM-1, and subsequently augments adhesion of cancer cells expressing sialyl Lewis antigen, a ligand of E-selectin
Catley et al., FEBS Lett 2003 : IL-1beta dependent activation of NF-kappaB mediates PGE2 release via the expression of cyclooxygenase-2 and microsomal prostaglandin E synthase ... These data conclusively demonstrate a substantial role for NF-kappaB in the co-ordinate induction of COX-2 , mPGES and in the corresponding release of PGE2 by IL-1beta
Okano et al., Lab Invest 2003 (Carcinoma, Hepatocellular...) : Furthermore, cFLIP over-expression activated nuclear factor (NF)-kappaB and cFLIP down-regulation attenuated NF-kappaB activation induced by TNF-alpha or TRAIL ... Furthermore, cFLIP over-expression activated nuclear factor (NF)-kappaB and cFLIP down-regulation attenuated NF-kappaB activation induced by TNF-alpha or TRAIL ... Furthermore, cFLIP over-expression activated nuclear factor (NF)-kappaB and cFLIP down-regulation attenuated NF-kappaB activation induced by TNF-alpha or TRAIL
Meier et al., Cell Microbiol 2003 : Using macrophages derived from mice lacking TLR2 expression, expressing defective TLR4 or both we found that A. fumigatus conidia and hyphae induce NF-kappaB translocation, release of pro-inflammatory molecules, like TNFalpha, and the chemoattractant MIP-2 in a TLR2- and TLR4 dependent manner
Lee et al., J Biol Chem 2003 : Saturated fatty acid ( lauric acid ) -induced NFkappaB activation was inhibited by a dominant negative mutant of TLR4 , MyD88, IRAK-1, TRAF6, or IkappaBalpha in macrophages ( RAW264.7 ) and 293T cells transfected with TLR4 and MD2 ... Saturated fatty acid ( lauric acid ) -induced NFkappaB activation was inhibited by a dominant negative mutant of TLR4, MyD88, IRAK-1, TRAF6 , or IkappaBalpha in macrophages ( RAW264.7 ) and 293T cells transfected with TLR4 and MD2 ... Saturated fatty acid ( lauric acid ) -induced NFkappaB activation was inhibited by a dominant negative mutant of TLR4, MyD88, IRAK-1 , TRAF6, or IkappaBalpha in macrophages ( RAW264.7 ) and 293T cells transfected with TLR4 and MD2 ... Saturated fatty acid ( lauric acid ) -induced NFkappaB activation was inhibited by a dominant negative mutant of TLR4, MyD88 , IRAK-1, TRAF6, or IkappaBalpha in macrophages ( RAW264.7 ) and 293T cells transfected with TLR4 and MD2 ... Saturated fatty acid ( lauric acid ) -induced NFkappaB activation was inhibited by a dominant negative mutant of TLR4, MyD88, IRAK-1, TRAF6, or IkappaBalpha in macrophages ( RAW264.7 ) and 293T cells transfected with TLR4 and MD2 ... Saturated fatty acid ( lauric acid ) -induced NFkappaB activation was inhibited by a dominant negative mutant of TLR4, MyD88, IRAK-1 , TRAF6, or IkappaBalpha in macrophages ( RAW264.7 ) and 293T cells transfected with TLR4 and MD2 ... LY294002, dominant negative ( DN ) phosphatidylinositol 3-kinase (PI3K), or AKT ( DN ) inhibited NFkappaB activation, p65 transactivation, and cyclooxygenase-2 (COX-2) expression induced by lauric acid or constitutively active ( CA ) TLR4 ... LY294002, dominant negative ( DN ) phosphatidylinositol 3-kinase (PI3K) , or AKT ( DN ) inhibited NFkappaB activation, p65 transactivation, and cyclooxygenase-2 (COX-2) expression induced by lauric acid or constitutively active ( CA ) TLR4 ... LY294002, dominant negative ( DN ) phosphatidylinositol 3-kinase (PI3K), or AKT ( DN ) inhibited NFkappaB activation, p65 transactivation , and cyclooxygenase-2 (COX-2) expression induced by lauric acid or constitutively active ( CA ) TLR4 ... LY294002, dominant negative ( DN ) phosphatidylinositol 3-kinase (PI3K), or AKT ( DN ) inhibited NFkappaB activation, p65 transactivation, and cyclooxygenase-2 (COX-2) expression induced by lauric acid or constitutively active ( CA ) TLR4 ... AKT ( DN ) blocked MyD88 induced NFkappaB activation, suggesting that AKT is a MyD88 dependent downstream signaling component of TLR4 ... AKT ( DN ) blocked MyD88 induced NFkappaB activation, suggesting that AKT is a MyD88 dependent downstream signaling component of TLR4 ... AKT ( CA ) was sufficient to induce NFkappaB activation and COX-2 expression ... DHA also suppressed NFkappaB activation induced by TLR4 ( CA ), but not MyD88 ( CA ) or AKT ( CA ), suggesting that the molecular targets of DHA are signaling components upstream of MyD88 and AKT ... DHA also suppressed NFkappaB activation induced by TLR4 ( CA ), but not MyD88 ( CA ) or AKT ( CA ), suggesting that the molecular targets of DHA are signaling components upstream of MyD88 and AKT ... DHA also suppressed NFkappaB activation induced by TLR4 ( CA ), but not MyD88 ( CA ) or AKT ( CA ), suggesting that the molecular targets of DHA are signaling components upstream of MyD88 and AKT
Cuschieri et al., Shock 2003 (MAP Kinase Signaling System...) : LPS stimulation led to mitogen activated protein kinase activation and translocation of activator protein-1 (AP-1) and nuclear factor (NF)-kappaB
Amtmann et al., Drugs Exp Clin Res 2003 (Endotoxemia) : C11AG blocked LPS stimulated sphingomyelin degradation and NF kappa B activation without interfering with p42 tyrosine phosphorylation ... C11AG blocked LPS stimulated sphingomyelin degradation and NF kappa B activation without interfering with p42 tyrosine phosphorylation
Kumar et al., Neoplasia (New York, N.Y.) 2003 (Prostatic Neoplasms) : Overexpression of constitutively active Akt , but not the kinase dead mutant of Akt, activated the basal NFkappaB transcriptional activity
Ma et al., Mol Pharmacol 2003 : Hydroquinone and tert-butyl hydroquinone, prototypes of phenolic antioxidants, block lipopolysaccharide (LPS) induced transcription of TNFalpha and a nuclear factor (NF)-kappaB mediated reporter gene expression, suggesting NF-kappaB as a target in the inhibition ... Analyses of the NF-kappaB activation pathway revealed that the antioxidants do not inhibit LPS induced activation of the IkappaB kinase activity, degradation of IkappaBalpha, or translocation of activated NF-kappaB into the nucleus, but they do block the formation of NF-kappaB/DNA binding complexes
Yang et al., Mol Pharmacol 2003 : Activation of D2R led to phosphorylation of c-Src at Tyr-418, and expression of a kinase-deficient c-Src inhibited D2R mediated NF-kappaB activation ... The D2R mediated NF-kappaB activation was not dependent on epidermal growth factor (EGF) receptor transactivation since 4- ( 3'-chloroanilino ) -6,7-dimethoxyquinazoline ( AG1478 ), an EGF receptor-selective tyrphostin used at 1 microM, blocked EGF induced NF-kappaB activation but not the quinpirole induced response ... The D2R mediated NF-kappaB activation was not dependent on epidermal growth factor (EGF) receptor transactivation since 4- ( 3'-chloroanilino ) -6,7-dimethoxyquinazoline ( AG1478 ), an EGF receptor-selective tyrphostin used at 1 microM, blocked EGF induced NF-kappaB activation but not the quinpirole induced response ... These results suggest that D2R mediated NF-kappaB activation requires Gbetagamma and c-Src , and possibly involves beta-arrestin 1
Kang et al., Mol Cell Biochem 2003 : PI3-kinase specific inhibitors, such as wortmannin and LY294003, substantially blocked both silica induced PI3-kinase and NF-kappaB activation ... Antioxidants, such as superoxide dismutase ( SOD ), N-acetylcysteine (NAC) and pyrrolidine dithiocarbamate ( PDTC ), blocked silica induced PI3-kinase activation, suggesting that reactive oxygen species may be important regulatory molecules in NF-kappaB activation by mediating PI3-kinase activation
Jana et al., J Neurochem 2003 : As the activation of both NF-kappaB and CCAAT/enhancer binding protein beta ( C/EBPbeta ) is important for the expression of TNF-alpha in microglial cells, we investigated the effect of p40 on the activation of NF-kappaB as well as C/EBPbeta ... Activation of NF-kappaB as well as C/EBPbeta by p40 and inhibition of p40 induced expression of TNF-alpha by Deltap65, a dominant negative mutant of p65, and DeltaC/EBPbeta, a dominant negative mutant of C/EBPbeta, suggests that p40 induces the expression of TNF-alpha through the activation of NF-kappaB and C/EBPbeta ... Activation of NF-kappaB as well as C/EBPbeta by p40 and inhibition of p40 induced expression of TNF-alpha by Deltap65, a dominant negative mutant of p65, and DeltaC/EBPbeta, a dominant negative mutant of C/EBPbeta, suggests that p40 induces the expression of TNF-alpha through the activation of NF-kappaB and C/EBPbeta ... Activation of NF-kappaB as well as C/EBPbeta by p40 and inhibition of p40 induced expression of TNF-alpha by Deltap65, a dominant negative mutant of p65, and DeltaC/EBPbeta, a dominant negative mutant of C/EBPbeta, suggests that p40 induces the expression of TNF-alpha through the activation of NF-kappaB and C/EBPbeta
Ohkita et al., Eur J Pharmacol 2003 : Our previous studies showed that a transcription factor nuclear factor-kappaB (NF-kappaB) plays an important role in endothelin-1 production ... In the present study, we investigated the possible involvement of NF-kappaB in the inhibitory regulation of endothelin-1 production by NO. 2- ( 4-carboxyphenyl ) -4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxide ( carboxy-PTIO ), which is a well-known NO scavenger, remarkably increased both endothelin-1 production and NF-kappaB activation in cultured vascular endothelial cells ... On the other hand, a spontaneous NO donor ( +/- ) - ( E ) -4-ethyl-2- [ ( E ) -hydroxyimino ] -5-nitro-3-hexanamide ( FK409 ) significantly attenuated the basal and tumor necrosis factor-alpha induced endothelin-1 production and NF-kappaB activation in endothelial cells
Lee et al., Lung Cancer 2003 (Lung Neoplasms) : Electrophoretic mobility shift assay showed that ad-IkappaBalpha blocked the activation of NF-kappaB induced by cisplatin and adriamycin
Koch et al., Am J Respir Cell Mol Biol 2004 : LPS increased NF-kappaB : DNA binding as examined by electrophoretic mobility shift assay, but this was not suppressed by PD 098059 or by SB 203580 ... LPS induced an increase in NF-kappaB activation as examined by p50 translocation assay without suppression by PD 098059 or by SB 203580 ... SN50 ( 100 microM ), an inhibitor of NF-kappaB translocation and the specific IKK-2-Inhibitor ( AS602868 ; 10 microM ), also prevented GM-CSF expression and release induced by LPS, indicating that GM-CSF release is NF-kappaB dependent ... GM-CSF expression and release in AM is controlled by NF-kappaB activation, and this is modulated by phosphorylation of MKK-1 and p38 MAP kinase acting on histone acetylation
Singer et al., Am J Physiol Lung Cell Mol Physiol 2003 (MAP Kinase Signaling System) : The experiments described here further these studies by examining p38 MAPK and NF-kappaB regulation of cyclooxygenase-2 (COX-2) expression in response to a complex inflammatory stimulus consisting of 10 ng/ml interleukin (IL)-1beta, tumor necrosis factor-alpha (TNF-alpha), and interferon (IFN)-gamma ... SB-203580 did not affect cytokine stimulated IkappaBalpha degradation, NF-kappaB nuclear binding activity, or NF-kappaB dependent signaling from the COX-2 promoter, indicating that p38 MAPK and NF-kappaB may affect COX-2 expression via separate signaling pathways ... These findings suggest that although p38 MAPK and NF-kappaB signaling regulate steady-state levels of COX-2 expression, p38 MAPK additionally affects stability of COX-2 mRNA in cytokine stimulated human airway myocytes
Demarchi et al., J Biol Chem 2003 : Glycogen synthase kinase-3 beta regulates NF-kappa B1/p105 stability
Hsieh et al., Cancer Gene Ther 2003 : Previous studies indicate that a strategy combining LMP1 mediated NF-kappaB activation and the HSV thymidine kinase/Ganciclovir ( HSVtk/GCV ) prodrug system leads to regression of tumor growth in nude mice
Kim et al., Mol Cells 2003 : IGF-1 increased the activity of signal transducer and activator of transcription 6 ( STAT6 ) in response to IL-4, and activated NF-(kappa)B ... IGF-1 increased the activity of signal transducer and activator of transcription 6 ( STAT6 ) in response to IL-4 , and activated NF-(kappa)B
Vona-Davis et al., J Surg Res 2003 (Endotoxemia) : Whether lipopolysaccharide (LPS) would stimulate nuclear factor (NF)-kappaB intranuclear translocation was also examined ... We hypothesized that IL-6 production is enhanced with LPS and cytokine challenge and that LPS stimulates NF-kappaB intranuclear translocation in a myogenic cell line ... LPS activated NF-kappaB showing p65 subunit cellular localization within 30 min ... Enhanced nuclear translocation of NF-kappaB in response to LPS was evident in a myogenic cell line
Nakamura et al., Exp Eye Res 2003 : The objective of the study was to investigate the effects of baicalin, baicalein, and wogonin ( plant flavonoids ) on interleukin-6 (IL-6) and interleukin-8 (IL-8) protein production, mRNA expression, and nuclear factor-kappaB (NF-kappaB) binding activities induced by interleukin-1beta (IL-1beta) in human retinal pigment epithelial cell line ( ARPE-19 ) cells
Tomczak et al., J Immunol 2003 (Colitis) : NF-kappa B is required within the innate immune system to inhibit microflora induced colitis and expression of IL-12 p40
Jensen et al., J Immunol 2003 : In reporter assays Pellino3 leads to activation of c-Jun and Elk-1, but not NF-kappaB
Li et al., J Immunol 2003 : In surviving cells, TRAIL activates NF-kappaB , induces expression of E-selectin , ICAM-1, and IL-8, and promotes adhesion of leukocytes ... In surviving cells, TRAIL activates NF-kappaB , induces expression of E-selectin, ICAM-1 , and IL-8, and promotes adhesion of leukocytes ... In surviving cells, TRAIL activates NF-kappaB , induces expression of E-selectin, ICAM-1, and IL-8 , and promotes adhesion of leukocytes ... In surviving cells, TRAIL activates NF-kappaB , induces expression of E-selectin, ICAM-1, and IL-8, and promotes adhesion of leukocytes ... In surviving cells, TRAIL activates NF-kappaB , induces expression of E-selectin, ICAM-1 , and IL-8, and promotes adhesion of leukocytes
Li et al., Infect Immun 2003 : Phosphoinositide 3 kinase mediates Toll-like receptor 4-induced activation of NF-kappa B in endothelial cells ... TLR4 signaling leads to activation of NF-kappa B and transcription of many genes involved in the inflammatory response ... Moreover, a dominant negative mutant of p85 ( p85-DN ) inhibited the NF-kappa B luciferase activity, IL-6 production, and I kappa B alpha degradation elicited by LPS and IL-1 beta but not that stimulated by tumor necrosis factor alpha ... The dominant negative mutant of Akt partially inhibited the NF-kappa B luciferase activity evoked by LPS and IL-1 beta ... Further, these studies suggest that PI3-kinase is an important mediator of LPS and IL-1 beta signaling leading to NF-kappa B activation in endothelial cells and that Akt is necessary but not sufficient for NF-kappa B activation by TLR4 ... Further, these studies suggest that PI3-kinase is an important mediator of LPS and IL-1 beta signaling leading to NF-kappa B activation in endothelial cells and that Akt is necessary but not sufficient for NF-kappa B activation by TLR4 ... Further, these studies suggest that PI3-kinase is an important mediator of LPS and IL-1 beta signaling leading to NF-kappa B activation in endothelial cells and that Akt is necessary but not sufficient for NF-kappa B activation by TLR4
Lagoumintzis et al., Infect Immun 2003 : NF-kappa B activation by GLP was rapid and followed the same time course as that by viable bacteria, suggesting that bacteria could directly activate NF-kappa B through GLP ... Activation of NF-kappa B and AP-1 by P. aeruginosa GLP may be involved not only in TNF-alpha induction but also in many of the inflammatory responses triggered in the course of infection with P. aeruginosa ... Activation of NF-kappa B and AP-1 by P. aeruginosa GLP may be involved not only in TNF-alpha induction but also in many of the inflammatory responses triggered in the course of infection with P. aeruginosa
Ziouzenkova et al., J Biol Chem 2003 (Inflammation) : Here we demonstrate that LDL ( - ) increases tumor necrosis factor alpha (TNFalpha) induced inflammatory responses through NF kappa B and AP-1 activation with corresponding increases in vascular cell adhesion molecule-1 ( VCAM1 ) expression ... LPL treated LDL ( - ) suppressed NF kappa B and AP-1 activation, increasing expression of the PPAR alpha target gene I kappa B alpha, although only in the genetic presence of PPAR alpha and with intact LPL hydrolysis
Barrett et al., Int J Pediatr Otorhinolaryngol 2003 (Chronic Disease...) : In response to LPS the NF-kappaB content increased with 25-33 %
Sakon et al., EMBO J 2003 : Importantly, TNF does not induce ROS accumulation or prolonged MAPK activation in wild-type MEFs, indicating that TRAF mediated NF-kappaB activation normally suppresses the TNF induced ROS accumulation that subsequently induces prolonged MAPK activation and necrotic cell death
Duran et al., EMBO J 2003 : Essential role of RelA Ser311 phosphorylation by zetaPKC in NF-kappaB transcriptional activation
Russell et al., Oncogene 2003 (Inflammation) : Indeed, we find that RP3 alone causes increases in nuclear NF-kappaB activity and secretion of MCP-1 and GM-CSF
Morishima et al., J Biol Chem 2003 (Arteriosclerosis) : NFkappaB regulates plasma apolipoprotein A-I and high density lipoprotein cholesterol through inhibition of peroxisome proliferator activated receptor alpha
Wen et al., J Biol Chem 2003 (MAP Kinase Signaling System) : Consistent with this, we found that TL1A significantly increased the production of c-IAP2 , a known NF-kappaB dependent anti-apoptotic protein, and that the NF-kappaB inhibitor or cycloheximide prevented its synthesis ... Consistent with this, we found that TL1A significantly increased the production of c-IAP2 , a known NF-kappaB dependent anti-apoptotic protein, and that the NF-kappaB inhibitor or cycloheximide prevented its synthesis
Santilli et al., J Biol Chem 2003 (Neoplasm Invasiveness) : Ectopic apolipoprotein J expression strongly inhibited NF-kappaB activity in human neuroblastoma cells and murine embryonic fibroblasts by stabilizing inhibitors of NF-kappaB ( IkappaBs ) ... Absence of apolipoprotein J causes reduction of IkappaB stability, a tumor necrosis factor dependent increase in NF-kappaB activity, increased transcription of the NF-kappaB target gene c-IAP and down-modulation of p53 protein
Loop et al., Anesthesiology 2003 : Thiopental mediated inhibition of NF-kappaB activation is due to the suppression of IkappaB kinase activity and depends at least in part on the thio-group of the barbiturate molecule
Okano et al., Oncol Rep 2003 (Carcinoma, Hepatocellular) : Fas stimulation activates NF-kappaB in SK-Hep1 hepatocellular carcinoma cells ... However, the role of Fas in induction of NF-kappaB activation in HCC cells is not well understood ... Fas stimulation induced NF-kappaB activation in a dose dependent manner in SK-Hep1 and HepG2 cell lines, but not in HLE cells
Shikama et al., Eur J Immunol 2003 : Caspase-8 and caspase-10 activate NF-kappaB through RIP, NIK and IKKalpha kinases ... Using small interfering RNA technology, we further demonstrate that the down-regulation of IKKalpha but not IKKbeta significantly inhibits PDCasp8 mediated NF-kappaB activation ... Using small interfering RNA technology, we further demonstrate that the down-regulation of IKKalpha but not IKKbeta significantly inhibits PDCasp8 mediated NF-kappaB activation
Janssens et al., FEBS Lett 2003 : MyD88 is an adapter protein that is involved in Toll-like receptor ( TLR ) - and interleukin-1 receptor (IL-1R) induced activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK) ... MyD88 is an adapter protein that is involved in Toll-like receptor ( TLR ) - and interleukin-1 receptor (IL-1R) induced activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK) ... MyD88 ( S ) is not able to activate NF-kappaB, and in contrast functions as a dominant negative inhibitor of TLR/IL-1R induced NF-kappaB activation ... MyD88 ( S ) is not able to activate NF-kappaB, and in contrast functions as a dominant negative inhibitor of TLR/IL-1R induced NF-kappaB activation ... Moreover, the regulated expression of a MyD88 splice variant which specifically interferes with NF-kappaB- but not AP-1 dependent gene expression implies an important role for alternative splicing in the fine tuning of TLR/IL-1R responses
Tapia et al., Free Radic Biol Med 2003 : It is concluded that T ( 3 ) -induced oxidative stress enhances the DNA binding activity of NF-kappaB and the NF-kappaB dependent expression of TNF-alpha and IL-10 genes ... It is concluded that T ( 3 ) -induced oxidative stress enhances the DNA binding activity of NF-kappaB and the NF-kappaB dependent expression of TNF-alpha and IL-10 genes
Longo et al., Circulation 2003 : Overexpression of CD40 , in a transient transfection system, activates the transcription factor NF-kappaB and upregulates IkappaBalpha, E-selectin, and tissue factor ( TF ) reporter activity ... In human coronary artery ECs ( HCAECs ), adenovirus mediated overexpression of A20 blocks physiological, CD40 induced activation of NF-kappaB , upstream of IkappaBalpha degradation ( Western blot ) and subsequently upregulation of ICAM-1, VCAM-1, and E-selectin ( flow cytometry )
Secchiero et al., J Leukoc Biol 2003 (Acute Disease...) : Moreover, NF-kappaB nuclear translocation induced by TRAIL but not by TNF-alpha was abrogated by z-IETD-fmk, a caspase-8-specific inhibitor ... Moreover, NF-kappaB nuclear translocation induced by TRAIL but not by TNF-alpha was abrogated by z-IETD-fmk, a caspase-8-specific inhibitor
Meldrum et al., J Surg Res 2003 (Myocardial Ischemia) : We hypothesized that CAB with cardiopulmonary bypass ( `` on-pump '' ) activates human myocardial NF-kappaB and increases TNF in the heart
Oshikawa et al., Exp Lung Res 2003 (MAP Kinase Signaling System) : LPS mediated TLR2 mRNA up-regulation was attenuated by inhibition of p38 kinase ( with SB203580 ) or nuclear factor (NF)-kappaB ( with sulfasalazine or SN-50 ), but not by inhibition of extracellular signal regulated kinase ( with PD98059 ) or c-Jun N-terminal kinase ( with SP600125 ), suggesting that LPS may induce TLR2 mRNA expression through p38 kinase and NF-kappaB activation ... LPS mediated TLR2 mRNA up-regulation was attenuated by inhibition of p38 kinase ( with SB203580 ) or nuclear factor (NF)-kappaB ( with sulfasalazine or SN-50 ), but not by inhibition of extracellular signal regulated kinase ( with PD98059 ) or c-Jun N-terminal kinase ( with SP600125 ), suggesting that LPS may induce TLR2 mRNA expression through p38 kinase and NF-kappaB activation
Dhanalakshmi et al., Int J Oncol 2003 (Neoplasm Metastasis...) : Accordingly, here we assessed the effect of GSE on constitutive and TNFalpha induced NF-kappaB DNA binding activity and apoptotic death in advanced human prostate carcinoma DU145 cells ... Constitutive and TNFalpha induced NF-kappaB DNA binding activity was inhibited by GSE at doses > or =50 microg/ml and treatments for > or =12 h
Punzón et al., Int Immunopharmacol 2003 : At the molecular level, EXPLY-37 did not inhibit the activation of the nuclear factor kappa B (NF-kappaB) transcription factor by TNF
Noguchi et al., Int Immunopharmacol 2003 : Electrophoretic mobility shift assay ( EMSA ) revealed that LPS activated nuclear factor-kappaB (NF-kappaB) in RAW264.7 cells and that this activation was suppressed by nafamostat mesilate
Janicke et al., Res Vet Sci 2003 : LPS and IFNgamma caused translocation of the transcription factor nuclear factor kappa B (NfkappaB) to the nucleus in equine cells suggesting the limited iNOS production seen in our cells is not due to deficits in this signal transduction pathway ... LPS and IFNgamma caused translocation of the transcription factor nuclear factor kappa B (NfkappaB) to the nucleus in equine cells suggesting the limited iNOS production seen in our cells is not due to deficits in this signal transduction pathway
Gagliardo et al., Am J Respir Crit Care Med 2003 (Asthma) : Freed NF-kappaB then induces expression of cytokines such as granulocyte-macrophage colony stimulating factor , interleukin-8, and regulated upon activation, normal T cell expressed and secreted
Banan et al., J Pharmacol Exp Ther 2003 : We reported that activation of nuclear factor-kappaB (NF-kappaB) via degradation of its endogenous inhibitor I-kappaBalpha is key to oxidant induced disruption of barrier integrity and that growth factor ( epidermal growth factor, EGF ) protects against this injury by stabilizing the cytoskeletal filaments ... In an attempt to investigate, at the molecular level, the fundamental events underlying EGF protection against oxidant disruption, we tested the intriguing hypothesis that EGF induced activation of PKC-zeta prevents oxidant induced activation of NF-kappaB and the consequences of NF-kappaB activation, namely, cytoskeletal and barrier disruption ... Our data indicated that relative to WT monolayers exposed only to oxidant, pretreatment with EGF protected cell monolayers by 1 ) increasing native PKC-zeta activity ; 2 ) decreasing several variables related to NF-kappaB activation [ NF-kappaB ( both p50 and p65 subunits ) nuclear translocation, NF-kappaB subunits activity, I-kappaBalpha degradation, and phosphorylation ] ; 3 ) increasing stable tubulin ( increased polymerized S2 tubulin and decreased monomeric S1 tubulin ) ; 4 ) maintaining the cytoarchitectural integrity of microtubules ; and 5 ) preventing hyperpermeability ( barrier disruption )
Aktan et al., Nitric Oxide 2003 : Gypenosides derived from Gynostemma pentaphyllum suppress NO synthesis in murine macrophages by inhibiting iNOS enzymatic activity and attenuating NF-kappaB mediated iNOS protein expression ... Inhibition of iNOS protein expression appears to be at the transcriptional level, since gypenosides decreased LPS induced NF-kappaB activity in a dose dependent manner ( P < 0.05 ), with significant inhibition achieved following pretreatment with 10 microg/mL gypenoside ... Taken together, these results suggest that gypenosides derived from G. pentaphyllum suppress NO synthesis in murine macrophages by inhibiting iNOS enzymatic activity and attenuating NF-kappaB mediated iNOS protein expression, thereby implicating a mechanism by which gypenosides may exert their therapeutic effects
Han et al., Am J Physiol Lung Cell Mol Physiol 2004 (Endotoxemia) : This decrease in bronchoalveolar barrier function was associated with upregulation of iNOS protein expression and NF-kappaB activation in lung tissue
El-Guendy et al., Mol Cell Biol 2003 : In the present study, structure-function analysis indicated that inhibition of NF-kappaB activity and apoptosis is dependent on Par-4 translocation to the nucleus via a bipartite nuclear localization sequence, NLS2 ... Interestingly, a 59-amino-acid core that included NLS2 but not the C-terminal leucine zipper domain was necessary and sufficient to induce Fas pathway activation, inhibition of NF-kappaB activity, and apoptosis
Jiang et al., Mol Cell Biol 2003 : During impaired protein folding and assembly in the endoplasmic reticulum ( ER ), phosphorylation of eIF2alpha by PEK ( Perk or EIF2AK3 ) is essential for induction of NF-kappaB transcriptional activity
Parcellier et al., Mol Cell Biol 2003 : HSP27 overexpression increases NF-kappaB nuclear relocalization, DNA binding, and transcriptional activity induced by etoposide, TNF-alpha , and interleukin 1beta ... HSP27 overexpression increases NF-kappaB nuclear relocalization, DNA binding, and transcriptional activity induced by etoposide, TNF-alpha, and interleukin 1beta
Lin et al., Mol Cell Biol 2003 : Although TRAF2 is known to be required for TNF induced JNK and NF-kappaB activation , the underlying mechanism of the increased sensitivity of TRAF2 null cells ( TRAF2 ( -/- ) ) to TNF induced apoptosis is not fully understood
Chen et al., J Cell Biochem 2003 : Our results suggest that NF-kappaB activation in PIMs followed by phagocytizing lipopolysaccharide resulted in the up-regulation of TNF-alpha , IL-1beta, IL-6, IL-8, and COX-2, which could be alleviated by dexamethasone ... Our results suggest that NF-kappaB activation in PIMs followed by phagocytizing lipopolysaccharide resulted in the up-regulation of TNF-alpha, IL-1beta , IL-6, IL-8, and COX-2, which could be alleviated by dexamethasone ... Our results suggest that NF-kappaB activation in PIMs followed by phagocytizing lipopolysaccharide resulted in the up-regulation of TNF-alpha, IL-1beta , IL-6, IL-8, and COX-2, which could be alleviated by dexamethasone ... Our results suggest that NF-kappaB activation in PIMs followed by phagocytizing lipopolysaccharide resulted in the up-regulation of TNF-alpha, IL-1beta, IL-6 , IL-8, and COX-2, which could be alleviated by dexamethasone ... Our results suggest that NF-kappaB activation in PIMs followed by phagocytizing lipopolysaccharide resulted in the up-regulation of TNF-alpha, IL-1beta, IL-6, IL-8 , and COX-2, which could be alleviated by dexamethasone ... Our results suggest that NF-kappaB activation in PIMs followed by phagocytizing lipopolysaccharide resulted in the up-regulation of TNF-alpha, IL-1beta, IL-6, IL-8, and COX-2 , which could be alleviated by dexamethasone
Cook et al., Circulation 2003 (Cardiomegaly) : Infection of cardiomyocytes with an adenoviral vector ( Ad ) encoding A20 inhibited tumor necrosis factor-alpha stimulated NF-kappaB signaling with an efficacy comparable to dominant negative inhibitor of kappa-B kinase beta ( dnIKKbeta )
Garat et al., Cytokine 2003 : In addition, icIL-1Ra1 inhibited nuclear translocation of NF-kappaB after IL-1 stimulation
Kobayashi et al., Biochem Biophys Res Commun 2003 : Stretch induced IL-6 secretion from endothelial cells requires NF-kappaB activation ... Two NF-kappaB inhibitors, pyrrolidine dithiocarbamanate ( PDTC ) and SN50, or antisense oligodeoxynucleotides for NF-kappaB p65 and p50 suppressed IL-6 mRNA expressions induced by continuous stretch ... In conclusion, continuous stretch induces IL-6 secretion from ECs, most likely through sequential activation of IKKs and NF-kappaB
Nakayama et al., Biochem Biophys Res Commun 2003 (MAP Kinase Signaling System) : Raf1 also regulates the activation of NF-kappaB
Corn et al., J Immunol 2003 : The role of NF-kappaB in IFN-gamma gene expression includes a modest decrease in Stat4 activation, T box expressed in T cell levels, and differentiation efficiency along with a more prominent postdifferentiation step ... Further, induced expression of Bcl-3 , a trans activating IkappaB-like protein, is decreased in T cells as a consequence of NF-kappaB inhibition ... Together, these findings indicate that NF-kappaB induction in T cells regulates efficient clonal expansion, Th1 differentiation, and IFN-gamma production by Th1 lymphocytes at a control point downstream from differentiation
Cenni et al., Biochem J 2003 : We also demonstrate that Akt activity is necessary for IL-1 dependent NF-kappaB transactivation, since a kinase-defective mutant of Akt impairs IRAK2- and MyD88 dependent, but not IRAK1 dependent, NF-kappaB activity, as monitored by a gene reporter assay ... We also demonstrate that Akt activity is necessary for IL-1 dependent NF-kappaB transactivation, since a kinase-defective mutant of Akt impairs IRAK2- and MyD88 dependent, but not IRAK1 dependent, NF-kappaB activity, as monitored by a gene reporter assay ... However, NF-kappaB binding to DNA was not affected by inhibition of Akt, indicating that Akt regulates NF-kappaB at a level distinct from the dissociation of p65 from IkappaBalpha and its translocation to the nucleus, possibly involving phosphorylation of the p65 transactivation domain
Tuñón et al., Biochem Pharmacol 2003 : Moreover, electrophoretic mobility shift assay experiments indicated that both drugs blocked the LPS induced activation of NF-kappaB
Shishodia et al., Cancer Res 2003 : The inhibition of NF-kappaB activation correlated with suppression of NF-kappaB dependent cyclin D1 , cyclooxygenase 2, and matrix metalloproteinase 9 expression ... The inhibition of NF-kappaB activation correlated with suppression of NF-kappaB dependent cyclin D1, cyclooxygenase 2 , and matrix metalloproteinase 9 expression
D'Angio et al., Am J Physiol Lung Cell Mol Physiol 2004 (Hyperoxia) : LPS , but not hyperoxia, stimulated nuclear translocation of NF-kappa B in both cell lines
Waris et al., J Biol Chem 2003 : Activation of NF-kappaB was blocked by a series of tyrosine kinase inhibitors but not by IkappaB kinase inhibitor BAY 11-7085
Loncar et al., Gut 2003 (Colitis) : and aims : Tumour necrosis factor alpha (TNF-alpha) induction of nuclear factor kappaB (NFkappaB) activation plays a major role in the pathogenesis of inflammatory bowel disease ( IBD )
Kwon et al., Biochem Biophys Res Commun 2003 : Inhibition of NF-kappaB nuclear localization by coexpression of a mutant IkappaBalpha protein blocked CpG-ODN induced transcription from a MIP-2 promoter-reporter construct, showing that NF-kappaB activation is required for MIP-2 gene expression in the CpG-ODN signaling pathway ... We also provided evidence that NF-kappaB and c-Jun contributes to the expression of MIP-2 gene in response to CpG-ODN, since ectopical expression of NF-kappaB and c-Jun in RAW 246.7 cells leads to dramatically increase the ability of CpG-ODN 1826 ( S ) in MIP-2 promoter activity
Hong et al., Mol Cell Biol 2003 : Expression of MIS in the testis is downregulated by tumor necrosis factor alpha through the negative regulation of SF-1 transactivation by NF-kappa B
Adam et al., Mol Cell Biol 2003 : Potentiation of tumor necrosis factor induced NF-kappa B activation by deacetylase inhibitors is associated with a delayed cytoplasmic reappearance of I kappa B alpha ... Importantly, TSA prolonged both TNF induced DNA binding activity and the presence of NF-kappa B in the nucleus
Tian et al., J Biol Chem 2003 : Remarkably, we found that activation of NF-kappaB by TNF-alpha selectively inhibited TCDD induced serine 2 phosphorylation in mouse liver cells, suggesting that residue-specific phosphorylation of RNA PII CTD at the cyp1a1 promoter is an important regulatory point upon which signal `` cross-talk '' converges
Ludwig et al., Oncogene 2003 : Overexpression of Grap-2 inhibits RET induced NF-kappaB activation, and cotransfection of Grap-2 significantly reduces focus formation induced by oncogenic RET in NIH 3T3 cells ... Overexpression of Grap-2 inhibits RET induced NF-kappaB activation , and cotransfection of Grap-2 significantly reduces focus formation induced by oncogenic RET in NIH 3T3 cells
Stroh et al., Oncogene 2003 : The purified and imported VP22-IkappaBalpha retained its function and efficiently inhibited both constitutive and inducible NF-kappaB activation
Trompouki et al., Nature 2003 : CYLD inhibits activation of NF-kappaB by the TNFR family members CD40, XEDAR and EDAR in a manner that depends on the deubiquitinating activity of CYLD ... Downregulation of CYLD by RNA mediated interference augments both basal and CD40 mediated activation of NF-kappaB ... Downregulation of CYLD by RNA mediated interference augments both basal and CD40 mediated activation of NF-kappaB ... The inhibition of NF-kappaB activation by CYLD is mediated , at least in part, by the deubiquitination and inactivation of TNFR associated factor 2 (TRAF2) and, to a lesser extent, TRAF6
Kovalenko et al., Nature 2003 : The tumour suppressor CYLD negatively regulates NF-kappaB signalling by deubiquitination
Kim et al., Am J Physiol Gastrointest Liver Physiol 2003 (Helicobacter Infections) : The suppression of NF-kappaB significantly increased caspase-3 activity and apoptosis in H. pylori infected MKN-45 and Hs746T gastric epithelial cell lines as well as primary gastric epithelial cells ... An NF-kappaB signaling pathway via NF-kappaB inducing kinase and IkappaB kinase-beta activation was found to be involved in the inhibition of apoptosis in H. pylori infected gastric epithelial cells
Aizawa et al., Circ Res 2003 : The effects of SNAP and CNP on NF-kappaB are mediated by cAMP dependent protein kinase (PKA) but not cGMP dependent protein kinase ( PKG ) based on the findings that the selective PKA inhibitor, PKI, abolished the effects of SNAP and CNP on NF-kappaB, whereas the PKG inhibitor Rp-8-Br-PET-cGMP had no effect ... The effects of SNAP and CNP on NF-kappaB are mediated by cAMP dependent protein kinase (PKA) but not cGMP dependent protein kinase ( PKG ) based on the findings that the selective PKA inhibitor, PKI, abolished the effects of SNAP and CNP on NF-kappaB, whereas the PKG inhibitor Rp-8-Br-PET-cGMP had no effect ... The effects of SNAP and CNP on NF-kappaB are mediated by cAMP dependent protein kinase (PKA) but not cGMP dependent protein kinase ( PKG ) based on the findings that the selective PKA inhibitor, PKI, abolished the effects of SNAP and CNP on NF-kappaB , whereas the PKG inhibitor Rp-8-Br-PET-cGMP had no effect
Guo et al., Blood 2003 : Inhibiting NF-kappaB did not affect either IL-1beta secretion or phenotypic maturation but rather sensitized DCs to Fas mediated apoptosis
Harper et al., J Biol Chem 2003 : Taken together, these results show that PKC activation specifically inhibits the recruitment of key obligatory death domain containing adaptor proteins to their respective membrane associated signaling complexes, thereby modulating TRAIL induced apoptosis and TNF induced NF-kappaB activation, respectively ... Taken together, these results show that PKC activation specifically inhibits the recruitment of key obligatory death domain containing adaptor proteins to their respective membrane associated signaling complexes, thereby modulating TRAIL induced apoptosis and TNF induced NF-kappaB activation , respectively
Miskolci et al., Arch Biochem Biophys 2003 : Furthermore, inhibitors of protein kinase C-delta (PKCdelta) and IkappaB kinase (IKK) inhibit the OA-induced activation of NFkappaB ... Furthermore, inhibitors of protein kinase C-delta (PKCdelta) and IkappaB kinase (IKK) inhibit the OA-induced activation of NFkappaB ... Collectively, our results indicate that in human neutrophils, the sustained activation of NFkappaB is regulated by a continuous phosphorylation and degradation of the nuclear IkappaBalpha
Marcheselli et al., J Biol Chem 2003 (Reperfusion Injury) : In addition, in neural cells in culture, this lipid messenger also inhibited both interleukin 1-beta induced NFkappaB activation and cyclooxygenase-2 expression
Sun et al., J Biol Chem 2003 : TNF-R1 activates both caspase mediated apoptosis and NF-kappaB transcription of anti-apoptotic factors
Wong et al., J Biomed Sci 2003 (Shock, Septic) : This review summarizes yet another new role of Ran - control of the activity of NF-kappaB in host immune responses
Han et al., J Neurosci Res 2003 : PMA and IFN-gamma synergistically enhanced activity of NF-kappaB , but not ERK
Huang et al., J Neurosci Res 2003 (Brain Ischemia...) : Our results demonstrate that NFkappaB phosphorylation is reduced in ICE KO mice, suggesting that ICE or IL-1 are involved in early NFkappaB phosphorylation ... Our results demonstrate that NFkappaB phosphorylation is reduced in ICE KO mice, suggesting that ICE or IL-1 are involved in early NFkappaB phosphorylation
Riedemann et al., Immunity 2003 (Sepsis) : We now report that in vitro exposure of neutrophils to C5a causes increased levels of IkappaBalpha, decreased NF-kappaB dependent gene transcription of TNFalpha , and decreased lipopolysaccharide (LPS) induced TNFalpha production
Tadros et al., Blood 2003 : ABIN-2 inhibits nuclear factor-kappaB (NF-kappaB) activity and is a possible effector of A20 regulation of NF-kappaB
Kwon et al., Arch Pharm Res 2003 : Sodium salicylate inhibits expression of COX-2 through suppression of ERK and subsequent NF-kappaB activation in rat ventricular cardiomyocytes ... IL-1beta and TNF-alpha can induce extracellular signal regulated kinase ( ERK ), IKK, IkappaB degradation and NF-kappaB activation
Kang et al., FEBS Lett 2003 : Moreover, silymarin suppressed the TNF-alpha induced DNA binding of NF-kappaB/Rel in HUVECs
Fan et al., Sheng Li Xue Bao 2003 : Nitric acid reductase method, spectrophotometry, reverse transcription-polymerase chain reaction ( RT-PCR ), immunofluorescence-interactive laser cytometer techniques and Western blotting were used respectively to detect NO contents, NOS activity, iNOS mRNA expression and the activation of NF-kappaB in CFs. AVP increased NO contents, NOS activity and iNOS mRNA expressions in a concentration dependent manner ; NF-kappaB was activated and mobilized from cytoplasm to nucleus in AVP induced CFs ; PDTC, one of the inhibitors of NF-kappaB, could inhibit aforementioned increments
Sugatani et al., J Cell Biochem 2003 : Activin A stimulates IkappaB-alpha/NFkappaB and RANK expression for osteoclast differentiation, but not AKT survival pathway in osteoclast precursors ... RANKL stimulation of RANK/TRAF6 signaling increases nuclear factor-kappaB (NFkappaB) nuclear translocation and activates the Akt/PKB cell survival pathway ... RANKL stimulation of RANK/TRAF6 signaling increases nuclear factor-kappaB (NFkappaB) nuclear translocation and activates the Akt/PKB cell survival pathway ... Moreover, activin A enhanced RANKL- and M-CSF stimulated nuclear translocation of NFkappaB
Yin et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2001 : [ Effect of mm-LDL on NF-kB activation in endothelial cell ] ... To investigate the signal transduction pathway of NF-kB activated by minimally modified low density lipoprotein ( mm-LDL ) in endothelial cells and the effect of NF-kB on platelet derived growth factor b (PDGFb) mRNA expression ... In addition, endothelial cells were transfected using PDGFb promoter-luciferase for reporter gene analysis or transfected with mut-NIK for slot blot analysis to study the effect of NF-kB on PDGFb mRNA expression ... mm-LDL may activate NF-kB through NIK-IKK beta pathway and promote PDGFb mRNA expression in endothelial cells
Matsuguchi et al., EMBO J 2003 : Lipopolysaccharide (LPS) is recognized by Toll-like receptor (TLR) 4 and activates NF-kappaB and a set of MAP kinases
Popkin et al., J Virol 2003 : MCMV infection blocked TNF-alpha induced nuclear translocation of NF-kappaB
Engelsma et al., Cytokine 2003 : Involvement of the transcription factor nuclear factor (NF)-kappaB in expression of IL-1beta1 and IL-1beta2 was demonstrated
Hiroi et al., Biochem J 2003 (Carcinoma...) : Activation of NF-kappaB in Ca9-22 cells restored the expression of IFNgamma stimulated CXCL9 and CXCL10 mRNAs ... Activation of NF-kappaB in Ca9-22 cells restored the expression of IFNgamma stimulated CXCL9 and CXCL10 mRNAs ... In contrast, inhibition of the constitutive NF-kappaB in HSC-2 cells by adenovirus mediated gene transfer of a dominant negative IkappaBalpha suppressed the IFNgamma induced expression of the CXCL9 and CXCL10 mRNAs ... These results indicate that constitutive NF-kappaB activity, which is often associated with tumour development, is required for the induced expression of CXCL9 and CXCL10 genes in human tumour cell lines in response to IFNgamma ... These results indicate that constitutive NF-kappaB activity, which is often associated with tumour development, is required for the induced expression of CXCL9 and CXCL10 genes in human tumour cell lines in response to IFNgamma
Ishibashi et al., Microb Pathog 2003 : Western blot analysis revealed that the activation of NF-kappa B by B. pertussis was preceded by degradation of I kappa B alpha , a major cytoplasmic inhibitor of NF-kappa B ... Pretreatment of the BEAS-2B cells with the NF-kappa B inhibitors pyrrolidine dithiocarbamate ( PDTC ), MG-132, and SN50 resulted in a marked decrease in B. pertussis induced ICAM-1 expression, implying the involvement of NF-kappa B in ICAM-1 expression
Kang et al., Toxicol Appl Pharmacol 2003 : These data suggest that FeTMPyP downregulates the proliferative activity by inhibition of p38 MAPK activation, NF-kappaB activation , and IL-2 secretion during mitogenic stimulation of thymocytes ... These data suggest that FeTMPyP downregulates the proliferative activity by inhibition of p38 MAPK activation, NF-kappaB activation , and IL-2 secretion during mitogenic stimulation of thymocytes
Lerner-Marmarosh et al., Arterioscler Thromb Vasc Biol 2003 (Inflammation) : Human umbilical vein endothelial cells preexposed to flow showed decreased tumor necrosis factor (TNF)-alpha induced c-Jun and NF-kappaB transcriptional activation ... TNF-alpha mediated JNK, c-Jun, and NF-kappaB activation required Src and SHP-2 activity ... TNF-alpha mediated JNK, c-Jun, and NF-kappaB activation required Src and SHP-2 activity ... Because MEKK3 and Gab1 are critical for TNF-alpha induced c-Jun and NF-kappaB activation, we determined the role of SHP-2 phosphatase activity in MEKK3 signaling ... Because MEKK3 and Gab1 are critical for TNF-alpha induced c-Jun and NF-kappaB activation, we determined the role of SHP-2 phosphatase activity in MEKK3 signaling ... Because MEKK3 and Gab1 are critical for TNF-alpha induced c-Jun and NF-kappaB activation, we determined the role of SHP-2 phosphatase activity in MEKK3 signaling ... A catalytically inactive form of SHP-2 increased MEKK3/Gab1 interaction and inhibited MEKK3 ( but not MEKK1 ) -mediated c-Jun and NF-kappaB activation ... A catalytically inactive form of SHP-2 increased MEKK3/Gab1 interaction and inhibited MEKK3 ( but not MEKK1 ) -mediated c-Jun and NF-kappaB activation ... A catalytically inactive form of SHP-2 increased MEKK3/Gab1 interaction and inhibited MEKK3 ( but not MEKK1 ) -mediated c-Jun and NF-kappaB activation
Miyakawa-Naito et al., J Biol Chem 2003 : Partial truncation of the NH2 terminus of Na, K-ATPase catalytic alpha1-subunit abolished Ca2+ oscillations and downstream activation of NF-kappaB
Lang-Lazdunski et al., J Vasc Surg 2003 (Disease Models, Animal...) : LIN prevented apoptotic neuronal cell death, Bax immunoreactivity of the pro-apoptotic protein Bax, and the nuclear transcription factor NF-kappaB
Sanchez et al., J Neurochem 2003 : A binding site for NF-kappaB was not required for Nos2 promoter activity
Wang et al., J Biol Chem 2003 : Consistent with this observation, interference with NF-kappaB activation by adenovirus mediated overexpression of dominant negative IKK-beta or IkappaB-alpha did not increase UV-induced apoptosis ... Consistent with this observation, interference with NF-kappaB activation by adenovirus mediated overexpression of dominant negative IKK-beta or IkappaB-alpha did not increase UV-induced apoptosis
Habraken et al., Oncogene 2003 : Differential involvement of the hMRE11/hRAD50/NBS1 complex, BRCA1 and MLH1 in NF-kappaB activation by camptothecin and X-ray ... Differential involvement of the hMRE11/hRAD50/NBS1 complex, BRCA1 and MLH1 in NF-kappaB activation by camptothecin and X-ray
Xiong et al., Alcohol 2003 (Liver Diseases, Alcoholic) : In cultured Kupffer cells isolated from normal rats, treatment with a lipophilic iron chelator, 1,2-dimethyl-3-hydroxypyrid-4-one ( L1 ), markedly reduced lipopolysaccharide (LPS) induced NF-kappaB activation and expression of tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 ... Moreover, the iron treatment increased TNF-alpha release and TNF-alpha promoter activity in a NF-kappaB dependent manner
Yorke et al., Eur J Cancer 2003 (Adenocarcinoma...) : Receptor activator of NF-kappaB ( RANK ) activation induces several survival signals including nuclear factor kappaB (NF-kappaB) and activation and upregulation of Bcl-xL
Hehlgans et al., Eur Cytokine Netw 2003 (Fibrosarcoma) : Activation of the lymphotoxin-beta receptor induces NFkappaB dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells ... Activation of the lymphotoxin-beta receptor induces NFkappaB dependent interleukin-6 and MIP-2 secretion in mouse fibrosarcoma cells ... MIP-2 and IL-6 secretion was NFkappaB dependent because IkappaB transfected cells released significantly reduced amounts of both mediators
Gukovskaya et al., Am J Physiol Gastrointest Liver Physiol 2004 : To elucidate the mechanism of sensitization, here we investigate the effect of ethanol on Ca ( 2+ ) - and PKC mediated pathways of CCK induced NF-kappaB activation using an in vitro system of rat pancreatic acini incubated with ethanol ... Ethanol augmented CCK-8 induced activation of NF-kappaB , similar to our in vivo findings with ethanol fed rats ... Pharmacological analysis showed that NF-kappaB activation by thapsigargin but not by CCK-8 is mediated through the calcineurin pathway and that the inhibitory effect of ethanol on the thapsigargin induced NF-kappaB activation could be through inhibiting this pathway ... Inhibitory analysis demonstrated that Ca ( 2+ ) -independent ( novel and/or atypical ) PKC isoforms are involved in NF-kappaB activation induced by both CCK-8 and PMA in cells treated and not treated with ethanol
Jung et al., FASEB J 2003 (Inflammation...) : IL-1 mediated NFkB dependent cyclooxygenases-2 (COX-2) expression served as a positive effector for HIF-1alpha induction
Hostager et al., J Biol Chem 2003 : Finally, we show that TRAF2 and TRAF6 have overlapping functions in CD40 mediated NF-kappaB activation and CD80 up-regulation
Lagunoff et al., Int Rev Immunol 2003 : Virally encoded genes inhibit the activation of caspase-8 by the TNF receptor and Fas ; activate NF-kappaB to increase expression of antiapoptotic genes ; inhibit interferon response ; bind to p53, thereby blocking p53 dependent apoptosis ; and interact with other pro- and antiapoptotic cellular genes ... Virally encoded genes inhibit the activation of caspase-8 by the TNF receptor and Fas ; activate NF-kappaB to increase expression of antiapoptotic genes ; inhibit interferon response ; bind to p53, thereby blocking p53 dependent apoptosis ; and interact with other pro- and antiapoptotic cellular genes ... Virally encoded genes inhibit the activation of caspase-8 by the TNF receptor and Fas ; activate NF-kappaB to increase expression of antiapoptotic genes ; inhibit interferon response ; bind to p53, thereby blocking p53 dependent apoptosis ; and interact with other pro- and antiapoptotic cellular genes ... Virally encoded genes inhibit the activation of caspase-8 by the TNF receptor and Fas ; activate NF-kappaB to increase expression of antiapoptotic genes ; inhibit interferon response ; bind to p53 , thereby blocking p53 dependent apoptosis ; and interact with other pro- and antiapoptotic cellular genes
O'Donnell et al., Int Rev Immunol 2003 : Reovirus binding to both cell-surface sialic acid and junctional adhesion molecule 1 is required for NF-kappaB activation and apoptosis
Robbins et al., Endocrinology 2003 : In addition, caspase-12 was cleaved, and phosphorylation of eukaryotic initiation factor 2alpha occurred, suggesting that an endoplasmic reticulum stress pathway was involved in addition to NF-kappaB induction of an extrinsic pathway, possibly mediated by TNF related apoptosis inducing ligand
Hassa et al., J Biol Chem 2003 : Furthermore, PARP-1 interacted directly with p300 and enhanced the interaction of NF-kappaB1/p50 to p300 ... Furthermore, PARP-1 interacted directly with p300 and enhanced the interaction of NF-kappaB1/p50 to p300
Weber et al., J Leukoc Biol 2003 : To investigate a potential interplay between TNF-alpha induced activation of p38 MAPK and NF-kappaB , the p38 MAPK inhibitor SB203580 and a dominant negative p38 MAPK mutant were used
Palaga et al., J Immunol 2003 : Inhibition of Notch activation dramatically decreases T cell proliferation in both CD4 and CD8 cells and blocks both NF-kappaB activity and IFN-gamma production in peripheral T cells
Deva et al., J Immunol 2003 (MAP Kinase Signaling System) : That NF-kappaB up-regulates p38 ( MAPK ) is novel and is in contradiction to earlier reports in which NF-kappaB was shown to inhibit p38 ( MAPK ) ... That NF-kappaB up-regulates p38 ( MAPK ) is novel and is in contradiction to earlier reports in which NF-kappaB was shown to inhibit p38 ( MAPK )
Wang et al., J Immunol 2003 : IL-6 induces NF-kappa B activation in the intestinal epithelia ... Given the crucial role of IL-6 in the pathogenesis of chronic intestinal inflammation, it is not known whether IL-6 activates NF-kappaB , a central mediator of intestinal inflammation ... Basolateral IL-6 stimulation results in a maximal induction of NF-kappaB activation and NF-kappaB nuclear translocation at 2 h. IL-6 induces polarized expression of ICAM-1, an adhesion molecule shown to be important in the neutrophil-epithelial interactions in IBD ... Using various deletion constructs of ICAM-1 promoter, we show that ICAM-1 induction by IL-6 requires the activation of NF-kappaB ... Using various deletion constructs of ICAM-1 promoter, we show that ICAM-1 induction by IL-6 requires the activation of NF-kappaB ... We also demonstrate that overexpression of SOCS-3, a protein known to inhibit STAT activation in response to IL-6, down-regulates IL-6 induced NF-kappaB activation and ICAM-1 expression ... In summary, we demonstrate the activation of NF-kappaB by IL-6 in intestinal epithelia and the down-regulation of NF-kappaB induction by SOCS-3
Takada et al., J Immunol 2003 : BA suppressed NF-kappaB activation induced by TNF , PMA, cigarette smoke, okadaic acid, IL-1, and H ( 2 ) O ( 2 ) ... BA suppressed NF-kappaB activation induced by TNF, PMA, cigarette smoke, okadaic acid, IL-1 , and H ( 2 ) O ( 2 )
Sohma et al., Alcohol Clin Exp Res 2003 (Glioma...) : Annexin IV overexpression augmented ethanol induced NF-kappa B activation
Kim et al., Biochem Pharmacol 2003 : Among the flavonoids examined, luteolin showed the most potent inhibition on lipopolysaccharide (LPS) stimulated NF-kappa B transcriptional activity in Rat-1 fibroblasts
Baxa et al., Biochem Pharmacol 2003 (Lymphoma, T-Cell) : When the induction of Delta I kappa B alpha was prevented, we detected no reduction of NF-kappa B levels by genistein ... When the induction of Delta I kappa B alpha was prevented, we detected no reduction of NF-kappa B levels by genistein ... These results support a direct role for Delta I kappa B alpha in the reduction of NF-kappa B by genistein ... These results support a direct role for Delta I kappa B alpha in the reduction of NF-kappa B by genistein
Dai et al., Cell cycle (Georgetown, Tex.) 2003 : Finally, the NF-kappaB inhibitor CAPE antagonized NaB- and PMA related NF-kappaB DNA binding as well as induction of p21CIP1 ... Together, these findings suggest that NF-kappaB plays an important functional role in mediating NaB induced p21CIP1 induction, G1 arrest, and maturation in human myelomonocytic leukemia cells, and that disruption of the NF-kappaB pathway causes cells to engage an alternative, apoptotic program
Fisette et al., J Biol Chem 2003 : We found the purified preparation of Lip to be a potent inflammatory mediator capable of inducing the release of the chemokine interleukin (IL)-8 and the cytokine IL-6 by immortalized human endocervical epithelial cells and the production of IL-8 and the activation of the transcription factor NF-kappaB by human embryonic kidney 293 (HEK) cells transfected with toll-like receptor (TLR) 2
Gelbmann et al., Gut 2003 (Colitis, Ulcerative...) : Inducible CD40 expression mediates NFkappaB activation and cytokine secretion in human colonic fibroblasts
Zhou et al., Zhejiang Da Xue Xue Bao Yi Xue Ban 2003 : Eucalyptus globulus oil inhibits the nuclear translocation of NF-kappaB induced by LPS in THP-1 cells
Channavajhala et al., J Biol Chem 2003 (MAP Kinase Signaling System) : In addition, we demonstrate that hKSR-2 co-immunoprecipitates with Cot and that co-expression of hKSR-2 with Cot significantly reduces Cot mediated MAPK and NF-kappaB activation ... In addition, we demonstrate that hKSR-2 co-immunoprecipitates with Cot and that co-expression of hKSR-2 with Cot significantly reduces Cot mediated MAPK and NF-kappaB activation ... This inhibition is specific to Cot, because Ras induced ERK and IkappaB kinase induced NF-kappaB activation are not significantly affected by hKSR-2 co-expression ... Taken together, these results indicate that hKSR-2 , a new member of the KSR family, negatively regulates Cot mediated MAP kinase and NF-kappaB pathway signaling
Lean et al., J Clin Invest 2003 (Bone Resorption) : Furthermore, in vitro NAC prevented osteoclast formation and NF-kappaB activation
Han et al., J Pharmacol Exp Ther 2003 (Inflammation) : Treatment of cytomix stimulated cells with NAD+ also blocked nuclear factor-kappaB (NF-kappaB) activation, inducible nitric-oxide synthase induction , and increased production of nitric oxide ( NO. )
Schreck et al., J Exp Med 1992 : The antioxidant PDTC also blocked the activation of NF-kappa B by bacterial lipopolysaccharide (LPS) , suggesting a role of oxygen radicals in the intracellular signaling of LPS
Kruppa et al., J Immunol 1992 : Inhibition of tumor necrosis factor (TNF) mediated NF-kappa B activation by selective blockade of the human 55-kDa TNF receptor ... mAb H398 is shown to efficiently block not only TNF- but also lymphotoxin mediated activation of NF-kappa B ... Together, the results of this study indicate that TR55 is both necessary and sufficient for mediating TNF activation of NF-kappa B
Pang et al., J Biol Chem 1992 (Carcinoma, Papillary...) : TNF-alpha activated NF-kappa B , a nuclear factor thought to mediate multiple actions of TNF-alpha, in these cells with a maximum effect observed after 30 min of treatment
Schütze et al., Cell 1992 : TNF activates NF-kappa B by phosphatidylcholine-specific phospholipase C-induced `` acidic '' sphingomyelin breakdown
Thanos et al., Cell 1992 (Tumor Virus Infections) : In this paper, we show that both NF-kappa B and the high mobility group protein I ( Y ) ( HMG I(Y) ) are required for virus induction of the human interferon-beta (IFN-beta) gene
Bird et al., Cytokine 1992 : IL-1 induced NF-kappa B activation was, however, relatively insensitive to inhibition by K252a or quercetin
Bose et al., J Interferon Cytokine Res 2003 (Paramyxoviridae Infections) : The antiviral cytokine IFN-alpha/beta ( IFN type I ) produced following viral infection as a result of activation of NF-kappaB or IRF-3 or both exerts an antiviral state by inducing the Janus kinases/signal transducer and activator ( Jak-Stat ) pathway
Huang et al., Biochem Biophys Res Commun 2003 : Identification of a novel serine/threonine kinase that inhibits TNF induced NF-kappaB activation and p53 induced transcription
Muroya et al., Biochem Biophys Res Commun 2003 : We found that the nuclear factor-kappaB (NF-kappaB) mediated induction of gamma-GCS by LPS ( 100 ng/ml ) was suppressed by a 48-h pre-treatment with oxLDL ( 50 micro/ml ), and this was due to a decrease in the DNA binding activity of NF-kappaB ... Together, these indicate that oxidative modification of NF-kappaB suppresses LPS induced expression of gamma-GCS gene in ox-LDL treated cells, suggesting an implication of oxLDL induced modulation of NF-kappaB signaling with atherosclerosis
Li et al., Biochem Biophys Res Commun 2003 : It has been shown that Casper can either promote or antagonize apoptosis and can activate the transcription factor NF-kappaB ... Overexpression of p105 and IkappaBgamma inhibited Casper induced NF-kappaB activation and potentiated Casper induced apoptosis ... Overexpression of p105 and IkappaBgamma inhibited Casper induced NF-kappaB activation and potentiated Casper induced apoptosis
Culmsee et al., J Neurosci 2003 (Brain Ischemia) : In cultured neurons DNA damaging compounds induced activation of p53 , whereas NF-kappaB activity declined significantly
Kitchens et al., J Exp Med 1992 : In contrast, LPS stimulated marked increases of NF-kappa B binding activity and IL-1 beta ... dLPS and its synthetic lipid A counterpart, LA-14-PP ( also known as lipid Ia, lipid IVa, or compound 406 ) strongly inhibited LPS induced NF-kappa B and IL-1 beta, yet neither antagonist inhibited the uptake of LPS via CD14 ... dLPS did not inhibit NF-kappa B responses to tumor necrosis factor (TNF) alpha or phorbol ester
Stylianou et al., J Biol Chem 1992 (Thymoma...) : IL1 induction of NF-kappa B was rapid ( optimal at 15-30 min ) and transient in both cell types ... The IL1 receptor antagonist (IL1ra) , which binds strongly to the type I receptor, inhibited the NF-kappa B response in both cell lines ... The type I receptor mediates the induction of NF-kappa B in response to IL1 via a signaling mechanism that still remains to be identified
Schreck et al., J Virol 1992 : In this study, we used the antioxidant pyrrolidine dithiocarbamate ( PDTC ) to investigate whether the activation of NF-kappa B by the viral transactivator Tax from human T-cell leukemia virus type I also depends on the production of reactive oxygen intermediates ... The Tax induced activation of the DNA binding activity of NF-kappa B in Jurkat T cells was potently suppressed by micromolar concentrations of PDTC
Brach et al., Cell Growth Differ 1992 (Lymphoma, Large B-Cell, Diffuse) : Activation of NF-kappa B by interleukin 2 in human blood monocytes ... We report here that interleukin 2 (IL-2) acts on human blood monocytes by enhancing binding activity of the transcription factor NF-kappa B to its consensus sequence in the 5 ' regulatory enhancer region of the IL-2 receptor alpha chain ( p55 ) ... Similarly, IL-2 activates NF-kappa B in the human monocytic cell line U 937, but not in resting human T-cells ... Enhanced NF-kappa B binding activity is followed by functional activation in that inducibility of the IL-2 receptor alpha chain is mediated by enhanced NF-kappa B binding and that a heterologous promoter containing the NF-kappa B consensus sequence ( -291 to -245 ) of the IL-2 receptor alpha chain gene is activated ... In addition, IL-2 is capable of increasing transcript levels of the p50 gene coding for the p50 subunit of the NF-kappa B transcription factor, whereas mRNA levels of the p65 NF-kappa B gene remained unchanged
Schmidt-Supprian et al., Immunity 2003 : The IkappaB kinase (IKK) complex , consisting of two kinases, IKK1/alpha and IKK2/beta, and the NEMO/IKKgamma regulatory subunit, mediates NF-kappaB activation by most known stimuli ... Thus, IKK induced NF-kappaB activation , mediated by either IKK1 or IKK2, is essential for the generation and survival of mature T cells, and IKK2 has an additional role in regulatory and memory T cell development ... Thus, IKK induced NF-kappaB activation, mediated by either IKK1 or IKK2 , is essential for the generation and survival of mature T cells, and IKK2 has an additional role in regulatory and memory T cell development
Zhang et al., J Immunol 2003 : RANKL stimulated IkappaB-alpha phosphorylation, IkappaB-alpha degradation and DNA binding ability of NF-kappaB were increased after over-expression of SHP-1 ( C453S )
Fujita et al., J Immunol 2003 : S- ( 2,3-bispalmitoyloxypropyl ) Cys-Gly-Asp-Pro-Lys-His-Pro-Lys-Ser-Phe ( FSL-1 ) derived from Mycoplasma salivarium stimulated NF-kappaB reporter activity in human embryonic kidney 293 (HEK293) cells transfected with Toll-like receptor 2 (TLR2) or cotransfected with TLR2 and TLR6, but not in HEK293 cells transfected with TLR6, in a dose dependent manner
Lee et al., Mol Cells 2003 : Astaxanthin inhibits nitric oxide production and inflammatory gene expression by suppressing I(kappa)B kinase dependent NF-kappaB activation ... Astaxanthin also suppressed the serum levels of NO, PGE2, TNF-alpha , and IL-1beta in LPS administrated mice, and inhibited NF-kappaB activation as well as iNOS promoter activity in RAW264.7 cells stimulated with LPS ... Astaxanthin also suppressed the serum levels of NO, PGE2, TNF-alpha, and IL-1beta in LPS administrated mice, and inhibited NF-kappaB activation as well as iNOS promoter activity in RAW264.7 cells stimulated with LPS ... This compound directly inhibited the intracellular accumulation of reactive oxygen species in LPS stimulated RAW264.7 cells as well as H2O2 induced NF-kappaB activation and iNOS expression
Chen et al., Blood 2004 : Inhibition of nuclear factor-kappaB (NF-kappaB) and mitogen activated protein kinase ( MAPK ) pathway signaling by CsA may be responsible for the CsA mediated effects on the regulation of chemokine receptor and cyclooxygenase-2 (COX-2) expression
Platten et al., Biochem Pharmacol 2003 : TNL fails to modulate LPS stimulated nuclear factor-kappaB (NF-kappaB) reporter gene activity and phosphorylation of inhibitory kappaB ( IkappaB ), indicating that NF-kappaB is not involved in the TNL mediated suppression of LPS induced iNOS expression ... TNL fails to modulate LPS stimulated nuclear factor-kappaB (NF-kappaB) reporter gene activity and phosphorylation of inhibitory kappaB ( IkappaB ), indicating that NF-kappaB is not involved in the TNL mediated suppression of LPS induced iNOS expression
Lukiw et al., Invest Ophthalmol Vis Sci 2003 (Anoxia) : VEGF expression appears to be regulated through dual interdependent mechanisms involving HIF-1 directly and indirectly through NF-kappaB mediated COX-2 expression and PGE ( 2 ) production
Giang et al., J Nat Prod 2003 : ent-Kaurane diterpenoids from croton tonkinensis inhibit LPS induced NF-kappaB activation and NO production ... These ent-kauranoids inhibited LPS induced NF-kappaB activation in murine macrophage RAW264.7 cells at IC50 values between 0.07 and 0.42 microM
Massberg et al., Thromb Res 2003 (Inflammation) : Platelet mediated MCP secretion by SMCs involved the activation and nuclear translocation of the transcription factor nuclear factor-kappaB (NF-kappaB)
Zhou et al., Zhonghua Bing Li Xue Za Zhi 2003 (Arteriosclerosis...) : To detect whether the activation of nuclear factor-kappa B (NF-kappaB) in endothelial cells induced by mm-LDL can promote platelet derived growth factor-B ( PDGF-B ) expression in vitro, and whether it is also present in hypercholesterolemic rats in vivo, influence of age on NF-kappaB and PDGF-B signal transduction pathway
Kiritoshi et al., Diabetes 2003 (Diabetes Mellitus, Experimental...) : Our results suggest that hyperglycemia increases mitochondrial ROS production, resulting in NF-kappaB activation, COX-2 mRNA induction , COX-2 protein production, and PGE2 synthesis ... Our results suggest that hyperglycemia increases mitochondrial ROS production, resulting in NF-kappaB activation, COX-2 mRNA induction , COX-2 protein production, and PGE2 synthesis
Carter et al., J Biol Chem 2003 : The catalytic activity of IKKbeta is stimulated by pathologic and physiologic inducers of NF-kappaB , such as the Tax oncoprotein and proinflammatory cytokines
Sola et al., J Biol Chem 2003 : In addition, UDCA inhibited TGF-beta1 induced degradation of nuclear factor kappaB (NF-kappaB) and its inhibitor IkappaB ( p < 0.05 )
Liu et al., EMBO J 2003 : This activity of NF-kappaB is mediated , at least in part, by the upregulation of Serine protease inhibitor 2A (Spi2A) , a potent inhibitor of cathepsin B
Kandouz et al., Prostaglandins Other Lipid Mediat 2003 (Prostatic Neoplasms) : The enzymatic product of arachidonic acid metabolism, 12 ( S ) -HETE, mediates the activation of NF-kappaB by the 12-LOX ... Considering the known involvement of MAP kinase pathway in NF-kappaB activation and that of 12 ( S ) -HETE in MAP kinase pathway, 12-LOX present in prostate cancer tissues may contribute to the constitutive activation of NF-kappaB in prostate cancer cells
Hilgendorff et al., Int J Clin Pharmacol Ther 2003 : All statins inhibited LPS induced NF-kappaB binding activity in Mo in a dose dependent manner
Silverman et al., J Biol Chem 2003 : Immune activation of NF-kappaB and JNK requires Drosophila TAK1
Lambert et al., J Nutr 2003 (Neoplasms) : Studies with human cancer cell lines have demonstrated that epigallocatechin-3-gallate ( EGCG ), a major tea polyphenol, inhibits mitogen activated protein kinases, cyclin dependent kinases, growth factor related cell signaling, activation of activator protein 1 (AP-1) and nuclear factor kappaB (NFkappaB) , topoisomerase I and matrix metalloproteinases as well as other potential targets
Su et al., Zhonghua Yi Xue Za Zhi 2003 (Acute Disease...) : 1.5, 3.0, 6.0, and 12.0 hours after the onset of experiment the amount of ascitic fluid was measured, blood was extracted from abdominal aorta, changes of pancreas was observed, pancreatic tissues were stained with HE, and flow cytometry ( FCM ) and enzyme linked immuno-sorbent assay ( ELISA ) were used to examine the content of TNF-alpha protein and activation of NF-kappaB ( number of positive cells/50 micro l ) in the pancreatic tissues
Tang et al., J Biol Chem 2003 : Our data suggest that LMP1-95-8 is a pseudo-substrate of SCFHOS/betaTrCP E3 ubiquitin ligase and that interaction between LMP1 and HOS restricts the extent of LMP1 induced NF-kappaB signaling
Martin et al., Inflammation 2003 (MAP Kinase Signaling System...) : Rhein inhibits interleukin-1 beta induced activation of MEK/ERK pathway and DNA binding of NF-kappa B and AP-1 in chondrocytes cultured in hypoxia : a potential mechanism for its disease modifying effect in osteoarthritis
Li et al., Chin Med J (Engl) 2003 : mRNA expression of proinflammatory cytokines ( IL-1 beta, IL-6, TNF-alpha, and MCP-1 ) and activation of NF-kappa B of HMC were measured using Reverse transcription-polymerase chain reaction ( RT-PCR ) and electrophoretic mobility shift assay ( EMSA ) respectively ... mRNA expression of proinflammatory cytokines ( IL-1 beta, IL-6, TNF-alpha , and MCP-1 ) and activation of NF-kappa B of HMC were measured using Reverse transcription-polymerase chain reaction ( RT-PCR ) and electrophoretic mobility shift assay ( EMSA ) respectively ... mRNA expression of proinflammatory cytokines ( IL-1 beta , IL-6, TNF-alpha, and MCP-1 ) and activation of NF-kappa B of HMC were measured using Reverse transcription-polymerase chain reaction ( RT-PCR ) and electrophoretic mobility shift assay ( EMSA ) respectively ... mRNA expression of proinflammatory cytokines ( IL-1 beta, IL-6 , TNF-alpha, and MCP-1 ) and activation of NF-kappa B of HMC were measured using Reverse transcription-polymerase chain reaction ( RT-PCR ) and electrophoretic mobility shift assay ( EMSA ) respectively
Wang et al., J Biol Chem 2003 (Colonic Neoplasms) : Activation of NF-kappa B contributes to cIAP-2 induction ; however, the cellular mechanisms regulating cIAP-2 expression have not been entirely defined ... Moreover, overexpression of PKC delta induced cIAP-2 promoter activity and increased NF-kappa B transactivation , suggesting regulation of cIAP-2 expression by a PKC delta/NF-kappa B pathway
Yang et al., J Biol Chem 2003 (Carcinoma, Hepatocellular) : Tumor necrosis factor alpha (TNFalpha) , which activates both NF-kappa B and AP-1, increased MAT2A expression in a dose- and time dependent manner, binding of both NF-kappa B and AP-1 to the MAT2A promoter and MAT2A promoter activity, with the latter effect blocked by site directed mutagenesis of the NF-kappa B and AP-1 binding sites ... Although blocking NF-kappa B had no influence on the ability of TNF alpha to increase AP-1 nuclear binding, blocking AP-1 with dominant negative c-Jun prevented the TNF alpha mediated increase in NF-kappa B binding ... In conclusion, both NF-kappa B and AP-1 are required for basal MAT2A expression in HepG2 cells and mediate the increase in MAT2A expression in response to TNF alpha treatment
Park et al., J Immunol 2003 : Functional effects of TNF-alpha on a human follicular dendritic cell line : persistent NF-kappa B activation and sensitization for Fas mediated apoptosis
Zamorano et al., J Immunol 2003 : We previously found that IL-4 and TNF-alpha cooperate in the activation of STAT6 and NF-kappaB , suggesting that these transcription factors are regulated by common intracellular signaling pathways ... We previously found that IL-4 and TNF-alpha cooperate in the activation of STAT6 and NF-kappaB , suggesting that these transcription factors are regulated by common intracellular signaling pathways ... To test this hypothesis, we analyzed the effect of known inhibitors of NF-kappaB on the activation of STAT6
Ge et al., J Immunol 2003 : NF-kappa B regulates BCL3 transcription in T lymphocytes through an intronic enhancer ... Together, these results demonstrate a critical role for NF-kappaB in BCL3 transcriptional up-regulation by TCR-mimetic signals
Deng et al., Cell 2003 : While activation of caspase 8 is required for TNFalpha induced apoptosis, and induction of NF-kappaB inhibits cell death, the precise function of JNK activation in TNFalpha signaling is not clearly understood
Chen et al., J Urol 2003 (Carcinoma, Transitional Cell...) : We evaluated the influence of androgens on BCG induced, NF-kappaB mediated IL-6 expression ... Two pharmacological androgen receptor blockers were used to determine if receptor blockade inhibited the effect of DHT on activation of the androgen response element, NF-kappaB signaling and BCG induced IL-6 expression ... DHT suppressed BCG induced, NF-kappaB mediated signaling and IL-6 expression in a dose dependent manner ... DHT down-regulates NF-kappaB mediated IL-6 expression by human TCC lines in response to BCG
Lloyd et al., Int J Oncol 2003 (Neoplasm Invasiveness...) : We have recently demonstrated that constitutively active nuclear factor-kappa B (NF-kappaB) is responsible for the increased secretion of uPA and that inhibition of NF-kappaB suppresses secretion of uPA and cell migration of highly invasive cancer cells
Gohda et al., Int J Oncol 2003 (Leukemia, T-Cell) : In the course of our screening for tumor necrosis factor-alpha (TNF-alpha) function inhibitors, conophylline, a vinca alkaloid isolated from the plant Ervatamia microphylla, was found to inhibit TNF-alpha induced NF-kappaB activation ... We studied the effect of conophylline on TNF-alpha induced NF-kappaB and JNK activations in human T-cell leukemia Jurkat cells
Kim et al., Br J Pharmacol 2003 : In conclusion, Rg3 relaxes vessels as a consequence of NO production, to which iNOS induction contributes, and iNOS induction by Rg3 accompanied NF-kappaB activation, which involves phosphorylation and degradation of I-kappaBalpha and nuclear translocation of p65 ... In conclusion, Rg3 relaxes vessels as a consequence of NO production, to which iNOS induction contributes, and iNOS induction by Rg3 accompanied NF-kappaB activation, which involves phosphorylation and degradation of I-kappaBalpha and nuclear translocation of p65
Mahabeleshwar et al., J Biol Chem 2003 (Anoxia...) : The molecular mechanism ( s ) by which hypoxia/reoxygenation ( H/R ) regulates p56lck dependent activation of NFkappaB through tyrosine phosphorylation of IkappaBalpha and modulates the expression of downstream genes that are involved in cell migration in human breast cancer cells are not well defined ... The molecular mechanism ( s ) by which hypoxia/reoxygenation ( H/R ) regulates p56lck dependent activation of NFkappaB through tyrosine phosphorylation of IkappaBalpha and modulates the expression of downstream genes that are involved in cell migration in human breast cancer cells are not well defined ... In this paper, we investigated the involvement of protein-tyrosine kinase p56lck in the redox regulated activation of NFkappaB following H/R in highly invasive ( MDA-MB-231 ) and low invasive ( MCF-7 ) breast cancer cells ... Transfection of these cells with wild type Lck but not with mutant Lck F394 followed by H/R induces the tyrosine phosphorylation of inhibitor of nuclear factor kappaB ( IkappaBalpha ) and transcriptional activation of NFkappaB , and these are inhibited by Lck inhibitors ... Luciferase reporter gene assay indicated that Lck induces NFkappaB dependent urokinase type plasminogen activator ( uPA ) promoter activity in presence of H/R ... To our knowledge, this is the first report that p56lck in presence of H/R regulates NFkappaB activation, uPA secretion, and cell motility through tyrosine phosphorylation of IkappaBalpha and further demonstrates an important redox regulated pathway for NFkappaB activation following H/R injury that is independent of IkappaB kinase/IkappaBalpha mediated signaling pathways
Hamaguchi et al., J Pharmacol Exp Ther 2003 : A protein tyrosine kinase inhibitor, genistein, completely inhibited TNF-alpha induced PAI-1 secretion, whereas an inhibitor of extracellular signal regulated kinase ( ERK ) kinase, 2'-amino-3'-methoxyflavone ( PD98059 ), and a nuclear factor-kappaB (NF-kappaB) inhibitor, emodin, partly inhibited TNF-alpha induced PAI-1 secretion
Chen et al., Biochem Biophys Res Commun 2003 : When overexpressed in 293 cells, NKAP activated NF-kappaB in a dose dependent manner ... Moreover, down-regulation of NKAP by antisense RNA significantly inhibited TNF- and IL-1 induced NF-kappaB activation ... Moreover, down-regulation of NKAP by antisense RNA significantly inhibited TNF- and IL-1 induced NF-kappaB activation ... Our findings suggest that NKAP is a novel nuclear regulator of TNF- and IL-1 induced NF-kappaB activation
Lu et al., Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2003 : bFGF stimulated the EC proliferation and enhanced the expression of NF-kappa B and ki-67 in nucleus ; TNP-470 and Dex suppressed EC proliferation induced by bFGF, and reduced the expression of NF-kappa B and ki-67 in nucleus ... bFGF stimulated the EC proliferation and enhanced the expression of NF-kappa B and ki-67 in nucleus ; TNP-470 and Dex suppressed EC proliferation induced by bFGF, and reduced the expression of NF-kappa B and ki-67 in nucleus ... TNP-470 and Dex inhibited EC proliferation stimulated by bFGF by inhibiting NF-kappa B
Scholz et al., Cardiovasc Res 2003 (Inflammation...) : Furthermore, the ERK 1/2 inhibitor, PD98059, and the p38 MAPK inhibitor, SB203580, markedly reduced 8-isoprostane induced IL-8 expression ( mRNA and protein ), while inhibition of NF-kappaB activation and translocation had no significant effect on IL-8 expression
Adams et al., Eur J Cardiovasc Prev Rehabil 2003 (Heart Failure) : Cell culture studies and promoter analysis demonstrated that the transcription factor nuclear factor kappa B (NF-kappaB) is essential for iNOS-expression
Fotin-Mleczek et al., J Biol Chem 2004 : Tumor necrosis factor receptor associated factor ( TRAF) 1 regulates CD40 induced TRAF2 mediated NF-kappaB activation ... Tumor necrosis factor receptor associated factor ( TRAF) 1 regulates CD40 induced TRAF2 mediated NF-kappaB activation ... Accordingly, TRAF1 inhibited CD40 dependent but not TNF-R1 dependent NF-kappaB activation ... Accordingly, TRAF1 inhibited CD40 dependent but not TNF-R1 dependent NF-kappaB activation ... Moreover, down-regulation of TRAF1 with small interfering RNAs enhanced CD40/CD40 ligand induced NF-kappaB activation but showed no effect on TNF signaling ... Moreover, down-regulation of TRAF1 with small interfering RNAs enhanced CD40/CD40 ligand induced NF-kappaB activation but showed no effect on TNF signaling ... Moreover, down-regulation of TRAF1 with small interfering RNAs enhanced CD40/CD40 ligand induced NF-kappaB activation but showed no effect on TNF signaling
Kobayashi et al., Hypertension 2003 (Hypertension) : Celiprolol activates eNOS through the PI3K-Akt pathway and inhibits VCAM-1 Via NF-kappaB induced by oxidative stress
Yang et al., Chin J Cancer 2003 (Adenocarcinoma...) : [ Role of eukaryotic initiation factor-4E (eIF-4E) in regulation of expression of NF-kappaB and its subsequent influence on transcription and activity of heparanase in human colon adenocarcinoma cell line ] ... The current study was designed to investigate the effect of eIF-4E on the expression and activity of NF-kappaB and observe how the activity level of NF-kappaB contributes , as a secondary effect, to the transcription of heparanase in human colon adenocarcinoma LS-174T cells ... The current study was designed to investigate the effect of eIF-4E on the expression and activity of NF-kappaB and observe how the activity level of NF-kappaB contributes, as a secondary effect, to the transcription of heparanase in human colon adenocarcinoma LS-174T cells ... Furthermore, this down regulation of the ubiquitous transcription factor NF-kappaB led to reduced transcription of the heparanase gene, and the transfected cells also showed a considerable decrease in heparanase protein and activity ... These results suggest that eIF-4E play an important role in translational regulation of NF-kappaB expression in LS-174T cells.NF-kappaB is an essential factor in the regulatory mechanisms of heparanase gene transcription, and the suppressed NF-kappaB activity in LS-174T cells may significantly reduce the transcriptional expression of heparanase that consequently leads to decreased heparanase protein expression and enzymatic activity
Pauleau et al., Mol Cell Biol 2003 : Macrophages responded normally to multiple Toll-like receptor agonists in terms of NF-kappaB target activation, mitogen activated protein kinase activation , and cytokine secretion
Yamashita et al., Curr Drug Targets Inflamm Allergy 2003 (Arthritis, Rheumatoid) : ATG and ATM at 10 muM did not inhibit NF-kappaB nuclear translocation, but with 20 h preincubation, ATG and ATM inhibited PGE ( 2 ) production and NF-kappaB nuclear translocation ... AF, ATG , and ATM did not affect the binding of NF-kappaB to its specific DNA
Cabrero et al., Curr Drug Targets Inflamm Allergy 2002 (Inflammation) : PPARalpha activation results in the repression of NF-kappaB signaling and inflammatory cytokine production in different cell-types
Matta et al., J Biol Chem 2003 : We demonstrate that receptor interacting protein and NF-kappaB inducing kinase are dispensable for vFLIP K13 induced NF-kappaB DNA binding and transcriptional activation ... On the other hand, vFLIP K13 induced NF-kappaB DNA binding activity is significantly reduced, although not absent, in cells deficient in IKK1, IKK2, and NEMO ... Furthermore, vFLIP K13 induced NF-kappaB transcriptional activity is only weakly present in IKK1-deficient cells and almost completely absent in those deficient in IKK2 and NEMO ... HHV8 vFLIP K13 induced NF-kappaB activation in IKK1- and IKK2-deficient fibroblasts could be rescued by wild type but not by the kinase-inactive mutants of IKK1 and IKK2, respectively ... Consistent with the above results, vFLIP K13 induced NF-kappaB activation could be effectively blocked by chemical inhibitors of the kinase activity of IKK1 and IKK2 ... Consistent with the above results, vFLIP K13 induced NF-kappaB activation could be effectively blocked by chemical inhibitors of the kinase activity of IKK1 and IKK2 ... Consistent with the above results, vFLIP K13 induced NF-kappaB activation could be effectively blocked by chemical inhibitors of the kinase activity of IKK1 and IKK2 ... Thus, a cooperative interaction of all three subunits of the IKK complex is required for maximal NF-kappaB activation via HHV8 vFLIP K13 ... Selective inhibitors of the IKK1 kinase activity may have a role in the treatment of disorders caused by abnormal NF-kappaB activation by HHV8 vFLIP K13
Besnard-Guerin et al., J Biol Chem 2004 : Beta-catenin was translocated into the nucleus, whereas the tumor necrosis factor induced nuclear translocation of NFkappaB was impaired
Ruland et al., Semin Immunol 2003 : We focus mainly on T cell receptor ( TCR ) -mediated NF-kappaB activation and draw parallels to B cell receptor (BCR) mediated NF-kappaB activation where appropriate
Todorov et al., J Biol Chem 2004 : Tumor necrosis factor-alpha activates NFkappaB to inhibit renin transcription by targeting cAMP-responsive element ... Tumor necrosis factor-alpha activates NFkappaB to inhibit renin transcription by targeting cAMP-responsive element ... Constitutive activation of NFkappaB suppressed renin gene transcription, but NFkappaB appeared not to target the NFkappaB binding sites in the renin promoter ... Our results strongly suggest that NFkappaB mediates the effect of TNFalpha on renin transcription targeting a CRE in the mouse renin promoter
Okano et al., Lab Invest 2003 (Carcinoma, Hepatocellular...) : Although cell toxicity was induced when PPARgamma was down-regulated in HepG2 cells, NF-kappaB suppression and XIAP down-regulation were not induced ... In SK-Hep1 cells, the ability of 15d-PGJ ( 2 ) to induce cell toxicity, NF-kappaB suppression, or XIAP down-regulation seemed to occur via a PPARgamma independent mechanism, but in HepG2 cells, NF-kappaB suppression by 15d-PGJ ( 2 ) was dependent on PPARgamma
Ching et al., DNA Cell Biol 2003 : The main transcript, which is ubiquitously expressed, encodes a protein that binds tumor necrosis factor receptor 1 ( TNF-R1 ) and downregulates TNF induced activation of NF-kappaB
Séguin et al., J Cell Physiol 2003 (Arthritis...) : In both prechondrocytes and articular chondrocytes, TNFalpha induced concentration dependent activation of MEK1/2 and NF-kappaB , but not p38 or JNK
Nagineni et al., J Cell Physiol 2003 (MAP Kinase Signaling System...) : VEGF mRNA levels and protein secretion induced by TGF-beta were significantly inhibited by SB203580 and U0126, inhibitors of MAP kinases, but not by staurosporine and PDTC, protein kinase C and NF-kappaB pathway inhibitors, respectively
Lim et al., J Biol Chem 2004 (Stomach Neoplasms) : Previously, we showed that the expression of Ku antigen , a DNA repair protein, is mediated by NF-kappaB in gastric cancer AGS cells ( Lim, J. W., Kim, H., and Kim, K. H. ( 2002 ) J. Biol. Chem. 277, 46093-46100 )
Krunkosky et al., Free Radic Biol Med 2003 : In contrast, either the proteasome inhibitor carbobenzoxy-L-leucy-L-leucy-L-leucinal ( MG 132 ) or the IkappaBalpha inhibitor BAY 11-7082 ablated TNFalpha induced ICAM-1 gene expression and MG132 inhibited TNFalpha induced NFkappaB complexes
Ohata et al., FEBS Lett 2003 (Carcinoma, Hepatocellular...) : In this study, we demonstrated that the expression of full HBV genome and HBx gene similarly stimulated the transcriptional activity of NF-kappaB in HuH-7 human hepatoma cells, and that interferon ( IFN ) -alpha as well as dominant negative mutant of IkappaB kinase-alpha effectively inhibited the HBx mediated NF-kappaB activation, but IFN-gamma did not
Dadgostar et al., FEBS Lett 2003 : More importantly, T3JAM and TRAF3 synergistically activate JNK but not nuclear factor (NF)-kappaB ... More importantly, T3JAM and TRAF3 synergistically activate JNK but not nuclear factor (NF)-kappaB
Attuwaybi et al., J Surg Res 2003 (Reperfusion Injury) : We have previously shown that regional intraischemic hypothermia ( IH ) protects against moderate gut I/R induced mucosal injury, is associated with decreased NF-kappaB activity and inducible nitric oxide synthase induction and preserves heme oxygenase-1 (HO-1) expression
Gobert et al., J Biol Chem 2004 : H. pylori HSP60 induced IL-6 mRNA expression, and NF-kappaB activation in RAW 264.7 cells was abrogated in the presence of MG-132, a proteasome inhibitor
Birkenkamp et al., Leukemia 2004 (Acute Disease...) : The constitutive NF-kappaB activity could so far not be ascribed to the autocrine production of growth factors or to mutations in the Flt3 receptor, since anti-GM-CSF, -IL-1, -IL6, -TNFalpha or the tyrosine kinase inhibitor AG1296 did not affect the NF-kappaB DNA binding activity ... The constitutive NF-kappaB activity could so far not be ascribed to the autocrine production of growth factors or to mutations in the Flt3 receptor, since anti-GM-CSF , -IL-1, -IL6, -TNFalpha or the tyrosine kinase inhibitor AG1296 did not affect the NF-kappaB DNA binding activity ... The constitutive NF-kappaB activity could so far not be ascribed to the autocrine production of growth factors or to mutations in the Flt3 receptor, since anti-GM-CSF, -IL-1 , -IL6, -TNFalpha or the tyrosine kinase inhibitor AG1296 did not affect the NF-kappaB DNA binding activity ... The constitutive NF-kappaB activity could so far not be ascribed to the autocrine production of growth factors or to mutations in the Flt3 receptor, since anti-GM-CSF, -IL-1, -IL6 , -TNFalpha or the tyrosine kinase inhibitor AG1296 did not affect the NF-kappaB DNA binding activity
Kwon et al., Biochem Biophys Res Commun 2003 : NF-kappaB dependent regulation of tumor necrosis factor-alpha gene expression by CpG-oligodeoxynucleotides
Chen et al., Am J Physiol Heart Circ Physiol 2004 : Expression of N17Rac1 inhibited TNF-alpha induced MCP-1 and NF-kappaB transcriptional activity ... Expression of N17Rac1 inhibited TNF-alpha induced MCP-1 and NF-kappaB transcriptional activity
Ikeda et al., J Cardiovasc Pharmacol 2003 : Addition of IL-1beta activated nuclear factor-kappaB (NFkappaB) in VSMCs, whereas CRP did not affect IL-1beta induced NFkappaB activation ... Addition of IL-1beta activated nuclear factor-kappaB (NFkappaB) in VSMCs, whereas CRP did not affect IL-1beta induced NFkappaB activation
Eliopoulos et al., Oncogene 2003 : LMP1 induced NF-kappaB transactivation is reduced in nf-kb2 ( -/- ) mouse embryo fibroblasts, suggesting that p100 processing contributes to LMP1 mediated NF-kappaB transcriptional effects
Oakley et al., Am J Pathol 2003 (Liver Cirrhosis) : NGF regulates nuclear factor (NF)-kappaB activity, reducing p50/p65 binding detected by electromobility shift assay and reduced NF-kappaB CAT reporter activities from both basal unstimulated levels and after NF-kappaB induction by tumor necrosis factor ... NGF regulates nuclear factor (NF)-kappaB activity, reducing p50/p65 binding detected by electromobility shift assay and reduced NF-kappaB CAT reporter activities from both basal unstimulated levels and after NF-kappaB induction by tumor necrosis factor
Joshi-Barve et al., Hepatology 2003 : This increase in IL-8 is independent of nuclear factor kappaB (NF-kappaB) activation and is associated with an increase in c-Jun N-terminal kinase (JNK) and activator protein-1 (AP-1) activity
Jiang et al., J Biol Chem 2004 : In cultured rat vascular smooth muscle cells, sustained activation of ERK is required for interleukin-1beta to persistently activate NF-kappaB ... Without ERK activation, interleukin-1beta induces only acute and transient NF-kappaB activation ... Without ERK activation, interleukin-1beta induces only acute and transient NF-kappaB activation ... The expression of these genes was inhibited when NF-kappaB activation was down-regulated by MG132, a proteasome inhibitor, or by overexpression of an I-kappaBalpha mutant that prevented both the transient and the persistent activation of NF-kappaB ... Thus, although NF-kappaB activation was essential for interleukin-1beta induction of each of the proteins studied, gene expression was differentially regulated by ERK and by the duration of NF-kappaB activation
Sakurai et al., J Cell Physiol 2004 : Overexpression of thioredoxin reductase 1 regulates NF-kappa B activation ... Furthermore, the overexpression of TrxR1 enhanced TNF alpha induced DNA binding activity of NF-kappa B and NF-kappa B-dependent gene expression ... The catalytic Sec residue of TrxR1, which is essential for reducing Trx, was required for this NF-kappa B activation, and aurothiomalate, an inhibitor of TrxR, suppressed TNF alpha induced activation of NF-kappa B and the expression of NF-kappa B-targeted proinflammatory genes such as E-selectin and cyclooxygenase-2
Tian et al., Ann Clin Lab Sci 2003 (Endotoxemia) : Treatment of rats with HES ( 3.75 and 7.5 ml/kg ) prevented LPS induced NF-kappaB activation, and inhibited , in a dose related manner, LPS induced TNF-alpha and CINC expression ... Treatment of rats with HES ( 3.75 and 7.5 ml/kg ) prevented LPS induced NF-kappaB activation, and inhibited, in a dose related manner, LPS induced TNF-alpha and CINC expression
Gustin et al., J Biol Chem 2004 : Phosphatidylinositol (PI) 3-kinase/Akt signaling activates NF-kappa B through pleiotropic, cell type-specific mechanisms ... Phosphatidylinositol (PI) 3-kinase/Akt signaling activates NF-kappa B through pleiotropic, cell type-specific mechanisms ... This study investigated the significance of PI 3-kinase/Akt signaling to tumor necrosis factor (TNF) induced NF-kappa B activation in transformed, immortalized, and primary cells ... Pharmacological inhibition of PI 3-kinase blocked TNF induced NF-kappa B DNA binding in the 293 line of embryonic kidney cells, partially affected binding in MCF-7 breast cancer cells, HeLa and ME-180 cervical carcinoma cells, and NIH 3T3 cells but was without significant effect in H1299 and human umbilical vein endothelial cells, cell types in which TNF activated Akt ... Pharmacological inhibition of PI 3-kinase blocked TNF induced NF-kappa B DNA binding in the 293 line of embryonic kidney cells, partially affected binding in MCF-7 breast cancer cells, HeLa and ME-180 cervical carcinoma cells, and NIH 3T3 cells but was without significant effect in H1299 and human umbilical vein endothelial cells, cell types in which TNF activated Akt ... Also, inhibitors of PI 3-kinase blocked NF-kappa B DNA binding in Ikk beta-/- but not Ikk alpha-/- or wild-type cells in which the ratio of IKK alpha to IKK beta is low ... Thus, noncoordinate expression of I kappa B kinases plays a role in determining the cell type-specific role of Akt in NF-kappa B activation
Prajapati et al., J Biol Chem 2004 : Protein phosphatase 2Cbeta association with the IkappaB kinase complex is involved in regulating NF-kappaB activity
Soond et al., Mol Cell Biol 2003 : Transient overexpression of TRUSS activated NF-kappaB and increased NF-kappaB activation in response to ligation of TNF-R1
Nawata et al., Oncogene 2003 (Leukemia, Myelogenous, Chronic, BCR-ABL Positive) : Expression of MEKK1km also reduced NF-kappaB activation, and inhibited antiapoptotic c-IAP1 and c-IAP2 mRNA expression in response to the genotoxin ... Expression of MEKK1km also reduced NF-kappaB activation, and inhibited antiapoptotic c-IAP1 and c-IAP2 mRNA expression in response to the genotoxin ... Expression of MEKK1km also reduced NF-kappaB activation, and inhibited antiapoptotic c-IAP1 and c-IAP2 mRNA expression in response to the genotoxin
Wang et al., Arterioscler Thromb Vasc Biol 2004 : The role of [ beta]-transducin repeat containing protein ( [ beta ] -TrCP ) in the regulation of NF-[kappa]B in vascular smooth muscle cells
Gründker et al., Reproductive biology and endocrinology : RB&E 2003 (Ovarian Neoplasms) : In addition GnRH activates nucleus factor kappaB (NFkappaB) and protects the cancer cells from apoptosis
Kim et al., Mol Immunol 2003 : Inhibition of NF-kappaB nuclear localization by co-expression of a mutant IkappaBalpha protein ( IkappaBalpha super repressor, IkappaBalphaSR ) blocked LPS induced transcription from a MIP-2 promoter-reporter construct, showing that NF-kappaB activation is required for MIP-2 gene expression in the LPS signaling pathway ... In summary, our data suggest that both NF-kappaB and c-Jun contribute to LPS induced mouse MIP-2 gene expression in RAW 264.7 cells
Antony et al., Exp Cell Res 2003 (Calcium Signaling) : Pharmacological inhibition of PKC isozymes and Ras revealed that the BCR induced activation of NF-kappaB requires conventional PKCbeta, whereas that of NFAT may involve non-conventional PKCdelta and Ras pathways ... Pharmacological inhibition of PKC isozymes and Ras revealed that the BCR induced activation of NF-kappaB requires conventional PKCbeta , whereas that of NFAT may involve non-conventional PKCdelta and Ras pathways
Hardaker et al., FASEB J 2004 (Inflammation...) : TNF-alpha induced CXCL10 expression in hASM was dependent on NFkappaB activation, and a salicylanilide NFkappaB inhibitor blocked the CXCL10 expression ... In contrast, IFN-gamma stimulation resulted in transient NFkappaB activation, and the inhibitor had little effect on CXCL10 expression
Verma et al., J Biol Chem 2004 : Nuclear role of I kappa B Kinase-gamma/NF-kappa B essential modulator ( IKK gamma/NEMO ) in NF-kappa B-dependent gene expression ... Nuclear role of I kappa B Kinase-gamma/NF-kappa B essential modulator ( IKK gamma/NEMO ) in NF-kappa B-dependent gene expression
Lee et al., Biochem Pharmacol 2003 : Further analysis revealed that manassantins A and B dose-dependently suppressed not only the induced NF-kappaB activation by overexpression of RelA/p65 , but also transactivation activity of RelA/p65 ... Further analysis revealed that manassantins A and B dose-dependently suppressed not only the induced NF-kappaB activation by overexpression of RelA/p65 , but also transactivation activity of RelA/p65 ... Furthermore, treatment of cells with these compounds prevented the TNF-alpha induced expression of anti-apoptotic NF-kappaB target genes Bfl-1/A1, a prosurvival Bcl-2 homologue, and resulted in sensitizing HT-1080 cells to TNF-alpha induced cell death
Weber et al., Am J Physiol Endocrinol Metab 2004 : In addition, these ligands impair IL-1 induced NF-kappaB and MAPK as well as IFN-gamma stimulated signal transducer and activator of transcription ( STAT ) 1 activation in beta-cells
Osipo et al., J Natl Cancer Inst 2003 (Breast Neoplasms) : Paradoxically, fulvestrant reversed this effect and stimulated MCF-7TAMLT tumor growth apparently through ERalpha mediated regulation of Fas, HER2/neu, and NF-kappaB
Park et al., J Biol Chem 2004 : Fas associated factor-1 inhibits nuclear factor-kappaB (NF-kappaB) activity by interfering with nuclear translocation of the RelA ( p65 ) subunit of NF-kappaB ... Fas associated factor-1 inhibits nuclear factor-kappaB (NF-kappaB) activity by interfering with nuclear translocation of the RelA ( p65 ) subunit of NF-kappaB ... Fas associated factor-1 inhibits nuclear factor-kappaB (NF-kappaB) activity by interfering with nuclear translocation of the RelA ( p65 ) subunit of NF-kappaB ... Here, we show that FAF1 is involved in negative regulation of NF-kappaB activation ... NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha, interleukin-1beta, and lipopolysaccharide was also inhibited by FAF1 overexpression ... NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha , interleukin-1beta, and lipopolysaccharide was also inhibited by FAF1 overexpression ... NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha, interleukin-1beta , and lipopolysaccharide was also inhibited by FAF1 overexpression ... Moreover, FAF1 suppressed NF-kappaB activation induced by transducers of diverse NF-kappaB activating signals such as TNF receptor associated factor-2 and -6, MEKK1, and IkappaB kinase-beta as well as NF-kappaB p65, one of the end point molecules in the NF-kappaB activation pathway, suggesting that NF-kappaB p65 might be a target molecule upon which FAF1 acts ... Moreover, FAF1 suppressed NF-kappaB activation induced by transducers of diverse NF-kappaB activating signals such as TNF receptor associated factor-2 and -6, MEKK1, and IkappaB kinase-beta as well as NF-kappaB p65, one of the end point molecules in the NF-kappaB activation pathway, suggesting that NF-kappaB p65 might be a target molecule upon which FAF1 acts ... Moreover, FAF1 suppressed NF-kappaB activation induced by transducers of diverse NF-kappaB activating signals such as TNF receptor associated factor-2 and -6, MEKK1, and IkappaB kinase-beta as well as NF-kappaB p65 , one of the end point molecules in the NF-kappaB activation pathway, suggesting that NF-kappaB p65 might be a target molecule upon which FAF1 acts
Monteleone et al., J Biol Chem 2004 (Crohn Disease) : In marked contrast, treatment of LPMC from patients with inflammatory bowel disease with TGF-beta1 did not reduce TNF induced NF-kappaB activation due to the overexpression of Smad7 ... Indeed inhibiting Smad7 by specific antisense oligonucleotides increased IkappaBalpha expression and reduced NF-kappaB p65 accumulation in the nucleus ... Pretreatment of normal human intestinal lamina propria mononuclear cells ( LPMC ) with transforming growth factor-beta1 ( TGF-beta1 ) resulted in a marked suppression of TNF-alpha induced NF-kappaB p65 accumulation in the nucleus, NF-kappaB binding DNA activity, and NF-kappaB dependent gene activation
Banan et al., Am J Physiol Cell Physiol 2004 : We hypothesized that EGF induced activation of PKC-beta1 prevents oxidant induced activation of NF-kappaB and the consequences of NF-kappaB activation, F-actin, and barrier dysfunction ... We hypothesized that EGF induced activation of PKC-beta1 prevents oxidant induced activation of NF-kappaB and the consequences of NF-kappaB activation, F-actin, and barrier dysfunction
Frelin et al., Oncogene 2003 (Leukemia, T-Cell) : Pharmacological blockade of the IKK2 kinase with AS602868, a specific inhibitor that competes with ATP binding, prevented TNF-alpha induced NF-kappaB activation in Jurkat leukemic T cells
Stewart et al., J Cell Physiol 2004 : By contrast, inhibition of NF-kappaB in the presence of TNF-alpha culminates in increased cell cycle progression, decreased gadd45beta expression and significant and precociously increased cell death, when compared with TNF-alpha alone
Rao et al., J Biol Chem 2004 : GSK 3beta inhibition increased both NFkappaB and beta-catenin activity associated with decreased IkappaB and increased beta-catenin levels ... The increase in COX 2 following GSK 3beta inhibition was entirely blocked by NFkappaB inhibition using mutant IkappaB adenovirus
Jeong et al., J Biol Chem 2004 : Depletion of TRP14 augmented the TNF-alpha induced phosphorylation and degradation of I kappa B alpha as well as the consequent activation of NF-kappa B to a greater extent than did Trx1 depletion ... Depletion of TRP14 augmented the TNF-alpha induced phosphorylation and degradation of I kappa B alpha as well as the consequent activation of NF-kappa B to a greater extent than did Trx1 depletion ... Depletion of TRP14 augmented the TNF-alpha induced phosphorylation and degradation of I kappa B alpha as well as the consequent activation of NF-kappa B to a greater extent than did Trx1 depletion
Kettritz et al., J Biol Chem 2004 : GM-CSF and IL-8 did not activate NF-kappaB in suspended neutrophils but rapidly activated NF-kappaB under adherent conditions on matrix, as shown by IkappaB kinase activity assay, IkappaBalpha degradation, electromobility shift assay, and quantitative reverse transcriptase-PCR ... GM-CSF and IL-8 did not activate NF-kappaB in suspended neutrophils but rapidly activated NF-kappaB under adherent conditions on matrix, as shown by IkappaB kinase activity assay, IkappaBalpha degradation, electromobility shift assay, and quantitative reverse transcriptase-PCR ... In contrast, TNF-alpha activated NF-kappaB both in suspended cells and adherent cells ... Specific beta ( 2 ) integrin blockade prevented NF-kappaB activation by GM-CSF and IL-8 on fibronectin ... Specific beta ( 2 ) integrin blockade prevented NF-kappaB activation by GM-CSF and IL-8 on fibronectin ... Co-stimulating CD18 and CD11b with activating antibodies resulted in NF-kappaB activation by GM-CSF and IL-8 in suspended cells ... Co-stimulating CD18 and CD11b with activating antibodies resulted in NF-kappaB activation by GM-CSF and IL-8 in suspended cells
Idel et al., Proc Natl Acad Sci U S A 2003 (Arteriosclerosis) : A candidate gene in this region is A20, which is involved in the feedback suppression of NFkappaB activation induced by tumor necrosis factor alpha (TNFalpha) ... NFkappaB reporter gene assays showed that this amino acid change results in less effective termination of TNFalpha stimulated NFkappaB activation by C57BL/6J-A20 ... In accordance, the TNFalpha induced expression of NFkappaB target genes ( A20, IkappaBalpha ) in vascular smooth muscle cells was prolonged in cells isolated from C57BL/6J compared with FVB/N mice
Maeda et al., Immunity 2003 : IkappaB kinase beta (IKKbeta) is required for NF-kappaB activation and suppression of TNFalpha mediated liver apoptosis ... While TNFR2 does not contribute to NF-kappaB activation, it is important for ConA induced JNK activation, which is augmented by the absence of IKKbeta
Glezer et al., Neuropharmacology 2003 : We have investigated the involvement of NMDA-NO pathway in LPS induction of NF-kappaB in CNS ... Dexamethasone caused a stronger reduction in LPS induction of NF-kappaB in CNS, demonstrating that MK-801 and 7-Ni act on a pathway that is responsible only by a fraction of the overall NF-kappaB activation
Song et al., Proc Natl Acad Sci U S A 2003 (Brain Neoplasms...) : Gene expression profiling studies showed that IIp45 consistently inhibited the expression of cell invasion associated genes, such as the transcriptional NFkappaB , and its downstream target gene, intercellular adhesion molecule 1
Liao et al., Mol Cancer Ther 2003 (Prostatic Neoplasms) : Finally, Trichodion, a potent nuclear factor-kappaB (NF-kappaB) inhibitor, could not affect LiCl induced TRAIL sensitization, although GSK-3beta inhibitors significantly blocked TRAIL reduced NF-kappaB activity in prostate cancer cells
Espevik et al., Scand J Infect Dis 2003 : Also, pharmacological disruption of the Golgi did not inhibit LPS induced NF-kappaB activation
Chandrasekar et al., J Biol Chem 2004 (Arteriosclerosis...) : In conclusion, CXCL16 is a potent and direct activator of NF-kappaB and induces kappa B-dependent proinflammatory gene transcription ... CXCL16 mediated NF-kappa B activation occurred via heterotrimeric G proteins, PI3K, PDK-1, Akt, and I kappa B kinase (IKK) ... However, treatment with pertussis toxin ( G ( i ) inhibitor ), wortmannin or LY294002 ( phosphatidylinositol 3-kinase ( PI3K inhibitors ) ), or Akt inhibitor or overexpression of dominant negative ( dn ) PI3K gamma, dnPDK-1, kinase-dead ( kd ) Akt, kdIKK-beta, dnIKK-gamma, dnI kappa B-alpha, or dnI kappa B-beta significantly attenuated CXCL16 induced NF-kappa B activation ... However, treatment with pertussis toxin ( G ( i ) inhibitor ), wortmannin or LY294002 ( phosphatidylinositol 3-kinase ( PI3K inhibitors ) ), or Akt inhibitor or overexpression of dominant negative ( dn ) PI3K gamma, dnPDK-1, kinase-dead ( kd ) Akt , kdIKK-beta, dnIKK-gamma, dnI kappa B-alpha, or dnI kappa B-beta significantly attenuated CXCL16 induced NF-kappa B activation ... However, treatment with pertussis toxin ( G ( i ) inhibitor ), wortmannin or LY294002 ( phosphatidylinositol 3-kinase ( PI3K inhibitors ) ), or Akt inhibitor or overexpression of dominant negative ( dn ) PI3K gamma , dnPDK-1, kinase-dead ( kd ) Akt, kdIKK-beta, dnIKK-gamma, dnI kappa B-alpha, or dnI kappa B-beta significantly attenuated CXCL16 induced NF-kappa B activation
Watabe et al., J Biol Chem 2004 : Caffeic acid phenethyl ester induces apoptosis by inhibition of NFkappaB and activation of Fas in human breast cancer MCF-7 cells ... Our findings demonstrate that NFkappaB inhibition is sufficient to induce apoptosis and that Fas activation plays a role in NFkappaB inhibition induced apoptosis in MCF-7 cells
Teare et al., Neuroreport 2003 : We investigated the ability of alpha-MSH to inhibit the activation of NF-kappaB in cultured rat primary olfactory ensheathing cells stimulated with tumour necrosis factor-alpha or gamma-interferon
Kuroki et al., Cancer Immunol Immunother 2003 : OK-432 and LPS activated nuclear factor kappa B (NF-kappaB) in imMo-DCs ... Both secretion of IL-12 p70 and IFN-gamma and activation of NF-kappaB induced by OK-432 were suppressed when imMo-DCs were pretreated with cytochalasin B ... Both secretion of IL-12 p70 and IFN-gamma and activation of NF-kappaB induced by OK-432 were suppressed when imMo-DCs were pretreated with cytochalasin B ... These results indicate that uptake of OK-432 by imMo-DCs is an early critical event for IL-12 p70 production and that NF-kappaB activation induced by OK-432 also contributes partially to IL-12 p70 production ... These results indicate that uptake of OK-432 by imMo-DCs is an early critical event for IL-12 p70 production and that NF-kappaB activation induced by OK-432 also contributes partially to IL-12 p70 production
Sato et al., Leuk Res 2004 (Leukemia) : However, persistent activation of NF-kappaB by lipopolysaccharide (LPS) led to the survival of leukemic cells against VP16 induced apoptosis
Kammanadiminti et al., FASEB J 2004 : The Gal-lectin increased TLR-2 gene transcription, and the half-life of the mRNA transcripts was 1.4 h. Inhibition of nuclear factor (NF)-kappaB suppressed TLR-2 mRNA induction by the Gal-lectin ... We conclude that the Gal-lectin activates NF-kappaB and MAP kinase signaling pathways in macrophages culminating in the induction of several genes including TLR-2 and hypothesize that this could have a significant impact on macrophage activation and contribute to amoebic pathogenesis
Mackenzie et al., FASEB J 2004 : EC, CT, and DP-B inhibited , in vitro, NF-kappaB binding to its DNA consensus sequence, but they had no effect on the binding activity of CREB or OCT-1 ... EC, CT, and DP-B inhibited , in vitro, NF-kappaB binding to its DNA consensus sequence, but they had no effect on the binding activity of CREB or OCT-1
Darville et al., Endocrinology 2004 (Insulinoma) : We have previously shown that cytokine induced iNOS expression is cycloheximide ( CHX ) sensitive and requires nuclear factor-kappa B (NF-kappa B) activation
Andreakos et al., Blood 2004 : Distinct pathways of LPS induced NF-kappa B activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of MyD88 and Mal/TIRAP ... How lipopolysaccharide (LPS) signals through toll-like receptors ( TLRs ) to induce nuclear factor (NF)-kappa B inflammatory cytokines in sepsis remains unclear ... We found that MyD88 and Mal/TIRAP are essential for LPS induced I kappa B alpha phosphorylation, NF-kappa B activation, and interleukin 6 (IL-6) or IL-8 production in fibroblasts and endothelial cells in a pathway that also requires IKK2 ... We found that MyD88 and Mal/TIRAP are essential for LPS induced I kappa B alpha phosphorylation, NF-kappa B activation, and interleukin 6 (IL-6) or IL-8 production in fibroblasts and endothelial cells in a pathway that also requires IKK2 ... We found that MyD88 and Mal/TIRAP are essential for LPS induced I kappa B alpha phosphorylation, NF-kappa B activation , and interleukin 6 (IL-6) or IL-8 production in fibroblasts and endothelial cells in a pathway that also requires IKK2 ... We found that MyD88 and Mal/TIRAP are essential for LPS induced I kappa B alpha phosphorylation, NF-kappa B activation, and interleukin 6 (IL-6) or IL-8 production in fibroblasts and endothelial cells in a pathway that also requires IKK2 ... In contrast, in macrophages neither MyD88, Mal/TIRAP , nor I kappa B kinase 2 (IKK2) are required for NF-kappa B activation or tumor necrosis factor alpha (TNF alpha), IL-6, or IL-8 production, although Mal/TIRAP is still involved in the production of interferon beta (IFN beta) ... In contrast, in macrophages neither MyD88, Mal/TIRAP , nor I kappa B kinase 2 (IKK2) are required for NF-kappa B activation or tumor necrosis factor alpha (TNF alpha), IL-6, or IL-8 production, although Mal/TIRAP is still involved in the production of interferon beta (IFN beta) ... In contrast, in macrophages neither MyD88, Mal/TIRAP, nor I kappa B kinase 2 (IKK2) are required for NF-kappa B activation or tumor necrosis factor alpha (TNF alpha), IL-6, or IL-8 production, although Mal/TIRAP is still involved in the production of interferon beta (IFN beta) ... In contrast, in macrophages neither MyD88 , Mal/TIRAP, nor I kappa B kinase 2 (IKK2) are required for NF-kappa B activation or tumor necrosis factor alpha (TNF alpha), IL-6, or IL-8 production, although Mal/TIRAP is still involved in the production of interferon beta (IFN beta) ... These observations demonstrate for the first time the existence of 2 distinct pathways of LPS induced NF-kappa B activation and cytokine production in human myeloid and nonmyeloid cells defined by selective utilization of TLR4, MyD88, Mal/TIRAP, and IKK2, and reveal a layer of complexity not previously expected
Takada et al., J Biol Chem 2004 : NF-kappaB reporter activity induced by TNF receptor 1, TNF receptor associated death domain, TNF receptor associated factor-2, NF-kappaB inducing kinase, and IkappaBalpha kinase, were all blocked by flavopiridol but not that activated by p65 ... NF-kappaB reporter activity induced by TNF receptor 1, TNF receptor associated death domain, TNF receptor associated factor-2 , NF-kappaB inducing kinase, and IkappaBalpha kinase, were all blocked by flavopiridol but not that activated by p65 ... NF-kappaB reporter activity induced by TNF receptor 1, TNF receptor associated death domain, TNF receptor associated factor-2, NF-kappaB inducing kinase, and IkappaBalpha kinase, were all blocked by flavopiridol but not that activated by p65
Kido et al., J Periodontal Res 2003 : NF-kappaB inhibitors suppressed P-LPS induced NF-kappaB binding activity and calprotectin release
Chun et al., Carcinogenesis 2004 : Nitric oxide induces expression of cyclooxygenase-2 in mouse skin through activation of NF-kappaB ... In conclusion, up-regulation of COX-2 by NO may be mediated by activation of NF-kappaB in mouse skin, which provides a molecular mechanism by which COX-2 is induced during tumor promotion
Ishitani et al., EMBO J 2003 (Inflammation) : TAB2 , a TAK1 binding protein, is involved in IL-1 induced NF-kappaB activation by physically linking TAK1 to TRAF6 ... TAB2, a TAK1 binding protein, is involved in IL-1 induced NF-kappaB activation by physically linking TAK1 to TRAF6 ... However, IL-1 induced activation of NF-kappaB is not impaired in TAB2-deficient embryonic fibroblasts
Middendorp et al., J Immunol 2003 : In contrast, transgenic expression of kinase-inactive K430R-Btk completely reconstituted lambda usage in Btk-deficient mice, but the defective modulation of pre-B cell surface markers, peripheral B cell survival, and BCR mediated NF-kappaB induction were partially corrected
Mehta et al., J Immunol 2003 (Inflammation) : HB-EGF dramatically suppressed NF-kappaB activity and IL-8 release and decreased NO production in cells pretreated with HB-EGF ... HB-EGF blocked NF-kappaB activation by inhibiting IkappaB kinase activation and IkappaB phosphorylation and degradation, thus interfering with NF-kappaB nuclear translocation, DNA binding activity, and NF-kappaB dependent transcriptional activity
Ruefli-Brasse et al., J Biol Chem 2004 : Furthermore, deficiency in Bcl10 dependent NF-kappaB activation could be rescued in Rip2-/- embryonic fibroblasts by exogenous wild-type Rip2 but not a kinase-dead mutant ... Together these data define an important role for Rip2 in TCR induced NF-kappaB activation and T-cell function and highlight the significance of post-translational modification of Bcl10 by Rip2 in T-cell signaling
Coats et al., Infect Immun 2003 : Surprisingly, P. gingivalis LPS weakly but significantly activated NF-kappaB in HMEC-1 cells in the absence of E. coli LPS, and the P. gingivalis LPS dependent agonism was blocked by transient expression of a dominant negative murine TLR4 ... Surprisingly, P. gingivalis LPS weakly but significantly activated NF-kappaB in HMEC-1 cells in the absence of E. coli LPS, and the P. gingivalis LPS dependent agonism was blocked by transient expression of a dominant negative murine TLR4
Yanai et al., J Infect Dis 2003 (Stomach Neoplasms) : Super-repressor IkappaBalpha or NF-kappaB inhibitor reduced c-IAP2 transactivation by H. pylori, and exogenous expression of c-IAP2 inhibited apoptosis seen with H. pylori
Grassl et al., Cell Microbiol 2003 (MAP Kinase Signaling System) : Using Clostridium difficile toxins that specifically inactivate small GTPases, and transfection of inhibitory proteins of the Rho-GTPases, we demonstrate that Rac1, but not Cdc42 or Rho , is required for activation of NF-kappaB by invasin ... Using Clostridium difficile toxins that specifically inactivate small GTPases, and transfection of inhibitory proteins of the Rho-GTPases, we demonstrate that Rac1, but not Cdc42 or Rho, is required for activation of NF-kappaB by invasin ... Using Clostridium difficile toxins that specifically inactivate small GTPases, and transfection of inhibitory proteins of the Rho-GTPases, we demonstrate that Rac1 , but not Cdc42 or Rho, is required for activation of NF-kappaB by invasin ... While NF-kappaB and JNK contribute to IL-8 transcription, p38 MAPK also acts through stabilization of IL-8 mRNA, as confirmed by quantitative RT-PCR and electrophoretic mobility shift assays
Hattori et al., Eur J Pharmacol 2003 : A pharmacological inhibitor of the phosphoinositide 3-kinase (PI3K)-Akt pathway, 2- ( 4-morpholinyl ) -8-phenyl-4H-1-benzopyran-4-one ( LY294002 ), inhibited phosphorylation of Akt and suppressed activation of NF-kappa B by attenuating the phosphorylation and degradation of I kappa B. LY294002 thereby inhibited LPS/IFN induced iNOS expression and NO production
Takahra et al., Int J Biochem Cell Biol 2004 : Induction of myofibroblast MMP-9 transcription in three-dimensional collagen I gel cultures : regulation by NF-kappaB , AP-1 and Sp1
Zoog et al., Mol Immunol 2004 : Upon cellular entry, Antp-CD40 activated NF-kappaB dependent transcription, induced proteolytic processing of p100 to the p52/NF-kappaB2 subunit, and increased expression of CD80 and CD54 on the surface of B cells ... Upon cellular entry, Antp-CD40 activated NF-kappaB dependent transcription, induced proteolytic processing of p100 to the p52/NF-kappaB2 subunit, and increased expression of CD80 and CD54 on the surface of B cells
Mohan et al., Atherosclerosis 2003 (Arteriosclerosis...) : LS, on the other hand, may down-regulate eNOS expression resulting in reduced NO release, and thereby maintain high glucose induced NF-kappaB DNA binding activity
Bharti et al., J Biol Chem 2004 : Suppression of RANKL induced NF-kappaB activation by dominant negative IkappaBalpha or by the NEMO-peptide had no effect on RANKL induced cell growth inhibition
Ikezoe et al., Mol Pharmacol 2003 (Shock, Septic) : In this study, we demonstrated that PC-SPES inhibited the LPS induced NF-kappaB reporter activity in RAW264.7 macrophages
Choi et al., Mol Pharmacol 2003 : In PC-12 cells, nerve growth factor (NGF), which activates nuclear factor-kappaB (NF-kappaB) , maintained RACK1 levels and promoted cell survival in serum-free medium ... Inhibitors of NF-kappaB activation blocked NGF stimulated survival and RACK1 expression, whereas transgenic expression of RACK1 promoted survival in cells deprived of serum and NGF ... Thus, RACK1 gene expression is induced by NF-kappaB and RACK1 contributes to NF-kappaB mediated cell survival
Kempuraj et al., Int Arch Allergy Immunol 2003 : Azelastine inhibits secretion of IL-6, TNF-alpha and IL-8 as well as NF-kappaB activation and intracellular calcium ion levels in normal human mast cells ... Azelastine inhibits secretion of IL-6 , TNF-alpha and IL-8 as well as NF-kappaB activation and intracellular calcium ion levels in normal human mast cells ... Azelastine inhibits secretion of IL-6, TNF-alpha and IL-8 as well as NF-kappaB activation and intracellular calcium ion levels in normal human mast cells ... Azelastine inhibited hCBMC secretion of IL-6, TNF-alpha and IL-8, possibly by inhibiting intracellular Ca2+ ions and NF-kappaB activation ... Azelastine inhibited hCBMC secretion of IL-6 , TNF-alpha and IL-8, possibly by inhibiting intracellular Ca2+ ions and NF-kappaB activation ... Azelastine inhibited hCBMC secretion of IL-6, TNF-alpha and IL-8 , possibly by inhibiting intracellular Ca2+ ions and NF-kappaB activation
Vlaeminck-Guillem et al., Med Sci (Paris) 2003 (Cell Transformation, Neoplastic...) : For instance, glucocorticoid and PPAR receptors repress the transcription activity of the pro-inflammatory factor NF-kappa B
Huang et al., Cell 2003 : Sequential modification of NEMO/IKKgamma by SUMO-1 and ubiquitin mediates NF-kappaB activation by genotoxic stress
Min et al., Biochem Biophys Res Commun 2003 : Furthermore, plasminogen stimulated CREB and NF-kappaB DNA binding activity, and this activation was also reduced by trolox and NAC ... Furthermore, plasminogen stimulated CREB and NF-kappaB DNA binding activity, and this activation was also reduced by trolox and NAC
Safronova et al., Inflamm Res 2003 (Anoxia...) : The analysis of IkappaB degradation and NF-kappaB translocation revealed that hypoxia did not affect IL-1beta activation of NF-kappaB
Iha et al., Oncogene 2003 : Second, using NEMO knockout mouse fibroblasts and 10 NEMO mutants, we found that different regions function in trans either to complement or to inhibit dominantly TNFalpha, IL-1beta, or Tax activation of NF-kappaB ... For instance, NEMO ( 1-245 amino acids ) supported Tax mediated NF-kappaB activation, but did not serve TNFalpha- or IL-1beta signaling
Komiya et al., Int J Mol Med 2004 (Disease Models, Animal...) : It appeared that AST-120 reduced NF-kappaB activation and possibly the activity of NF-kappaB dependent pathways of interstitial inflammation including MCP-1 expression and macrophage infiltration
Hoshiya et al., Mol Cell Endocrinol 2003 (Breast Neoplasms...) : In both breast and prostate cancer cells, MIS activated NFkB DNA binding activity and induced IEX-1, an immediate early gene which regulates cell growth and apoptosis
Alzoghaibi et al., Am J Physiol Gastrointest Liver Physiol 2004 (Crohn Disease) : In contrast, TNF-alpha markedly increased activation of NF-kappaB in both normal and Crohn 's cells ... We speculate that AA metabolites increased IL-8 production by enhancing NF-kappaB dependent transcription of IL-8
Gennari et al., J Mol Biol 2004 : Further assessment of the biological activity of the anti-TES1 intrabody pools demonstrated that they were all able to selectively block F-LMP1 induced NFkappaB activity that was mediated through the TES1-site and to bind LMP1 protein with high efficiency
Park et al., J Biol Chem 2004 : Transfections with dominant negative constructs demonstrated that TLR 2 and TLR 4 were both involved in HMGB1 induced activation of NF-kappaB ... Transfections with dominant negative constructs demonstrated that TLR 2 and TLR 4 were both involved in HMGB1 induced activation of NF-kappaB ... Transfections with dominant negative constructs demonstrated that TLR 2 and TLR 4 were both involved in HMGB1 induced activation of NF-kappaB
Huang et al., Nat Immunol 2004 : Furthermore, MEKK3 is crucial for IL-1- and LPS induced activation of NF-kappaB and JNK-p38 but not ERK, indicating that MAPKs are differentially activated during IL-1R-TLR4 signaling ... Furthermore, MEKK3 is crucial for IL-1- and LPS induced activation of NF-kappaB and JNK-p38 but not ERK, indicating that MAPKs are differentially activated during IL-1R-TLR4 signaling
Samanta et al., J Biol Chem 2004 (Breast Neoplasms...) : In this study, we established cell model systems to examine whether stable expression of MEKK3 could lead to increased NFkappaB activity and confer resistance to apoptosis ... Thus, our results established that elevated expression of MEKK3 appears to be a frequent occurrence in breast and ovarian cancers and that overexpression of MEKK3 in cells leads to increased NFkappaB activity and increased expression of cell survival factors and ultimately contributes to their resistance to apoptosis
O'Connor et al., J Immunol 2003 : Transfection of full-length CIAS1 or either of two shorter, naturally occurring isoforms dramatically inhibited TNF-alpha induced activation of NF-kappaB reporter activity ... Transfection of full-length CIAS1 or either of two shorter, naturally occurring isoforms dramatically inhibited TNF-alpha induced activation of NF-kappaB reporter activity
Cowland et al., J Immunol 2003 : TNF-alpha activation of NF-kappaB , in contrast, did not increase NGAL synthesis, even though induced binding of NF-kappaB to the NGAL promoter was observed in vitro ... TNF-alpha activation of NF-kappaB , in contrast, did not increase NGAL synthesis, even though induced binding of NF-kappaB to the NGAL promoter was observed in vitro
Mancini et al., Neurosci Lett 2003 : These data indicate that therapeutic concentrations of acetaminophen induce an inhibition of IL-1beta dependent NF-kappaB nuclear translocation
Boyer et al., Mol Biol Cell 2004 : The novelty of these findings is in respect to the demonstration that the correct positioning at the ruffling membranes is crucial for the subsequent series of events that leads to IkBalpha proteasomal degradation and the resultant activation of NF-kappaB
Seki et al., Metabolism 2003 (Arteriosclerosis) : To examine possible mechanisms for this effect, we studied nuclear factor-kappa B (NF-kappaB) activation and the tyrosine phosphorylation pathway ; AGE stimulated NF-kappaB activity, but phosphorylation of the platelet derived growth factor ( PDGF ) receptor was unchanged
Kontou et al., Biol Chem 2003 (Chromosomal Instability...) : Cotransfection of the NF-kappaB dependent CAT plasmid with the Trx/nuc-plasmid into FA fibroblasts increased the CAT expression to almost that of control cells, indicating that in this model system with diminished thioredoxin content NF-kappaB requires thioredoxin for binding to its specific promotor ... Cotransfection of the NF-kappaB dependent CAT plasmid with the Trx/nuc-plasmid into FA fibroblasts increased the CAT expression to almost that of control cells, indicating that in this model system with diminished thioredoxin content NF-kappaB requires thioredoxin for binding to its specific promotor
Le Page et al., Biol Chem 2003 (Inflammation) : In RAW264.7 macrophages the inducers of NF-kappaB activate the production of reactive oxygen species and three mitogen activated protein kinases ( MAPK ), the extracellular signal regulated kinase ( ERK ), the c-jun N-terminal kinase/stress activated protein kinase ( JNK ), and p38 ... In RAW264.7 macrophages the inducers of NF-kappaB activate the production of reactive oxygen species and three mitogen activated protein kinases ( MAPK ), the extracellular signal regulated kinase ( ERK ), the c-jun N-terminal kinase/stress activated protein kinase ( JNK ), and p38
Cardoso et al., Free radical research 2003 (Alzheimer Disease) : We observed that the NF-kB inhibitor, PDTC, prevented SOD overexpression after A beta treatment ... In this study, we analyzed if NF-kB activation prevented cells from caspases-activation and we also observed that inhibition of NF-kB by PDTC induced an increase in caspase-3 and caspase-6 activation
Kang et al., Cancer Lett 2004 : Treatment of AAF caused a dose related inhibition of LPS induced NF-kappaB/Rel transcriptional activation, while the transcriptional activation of AP-1, CRE and NF-IL6 was not affected by AAF ... Furthermore, LPS induced NF-kappaB/Rel DNA binding was also suppressed by AAF treatment ... These results suggest that AAF inhibits IL-1beta gene expression by blocking NF-kappaB/Rel activation
Eickhoff et al., J Biol Chem 2004 (Cytomegalovirus Infections) : A stable inhibitor of NF-kappaB ( IkappaB ) reverses the RICK inhibitory effect, and activation of NF-kappaB by IkappaB kinase beta expression is inhibitory to HCMV, demonstrating that NF-kappaB activation is part of a potent anti-HCMV response
Fang et al., J Biol Chem 2004 (Ovarian Neoplasms) : The optimal response of the IL-8 gene promoter to LPA relied on binding sites for NF-kappaB and AP-1, two transcription factors that were strongly activated by LPA in ovarian cancer cell lines
Wang et al., J Bone Miner Res 2003 : 12-O-tetradecanoylphorbol-13-acetate ( TPA ) inhibits osteoclastogenesis by suppressing RANKL induced NF-kappaB activation ... Using a RAW ( 264.7 ) cell culture system and assays for NF-kappaB nuclear translocation, NF-kappaB reporter gene activity, and MAPK assays, we demonstrated that TPA inhibits osteoclastogenesis through the suppression of RANKL induced NF-kappaB activation ... Assays for NF-kappaB nuclear translocation, NF-kappaB reporter gene activity, protein kinase activity, and Western blotting were used to examine the effects of TPA on RANKL induced NF-kappaB , c-Jun N-terminal kinase (JNK), and MEK/ERK and p38 signal transduction pathways ... TPA alone had little effect on NF-kappaB activation in RAW ( 264.7 ) cells, but it suppresses the RANKL induced NF-kappaB activation in a dose dependent fashion ... Interestingly, the suppressive effect of TPA on RANKL induced NF-kappaB activation was prevented by a conventional PKC inhibitor, Go6976 ... Given that NF-kappaB activation is obligatory for osteoclast differentiation, our studies imply that inhibition of osteoclastogenesis by TPA is, at least in part, caused by the suppression of RANKL induced activation of NF-kappaB during an early stage of osteoclastogenesis
Tsuchiya et al., J Hepatol 2004 (Endotoxemia...) : Electrophoretic mobility shift assay revealed that PFD inhibited the activation of transcription factor nuclear factor-kappa B (NF-kappa B) induced by LPS ... Electrophoretic mobility shift assay revealed that PFD inhibited the activation of transcription factor nuclear factor-kappa B (NF-kappa B) induced by LPS
Luftig et al., Proc Natl Acad Sci U S A 2003 : Epstein-Barr virus latent membrane protein 1 activation of NF-kappaB through IRAK1 and TRAF6 ... Epstein-Barr virus latent membrane protein 1 activation of NF-kappaB through IRAK1 and TRAF6 ... Epstein-Barr virus latent membrane protein 1 (LMP1) activation of NF-kappaB is critical for Epstein-Barr virus infected B lymphocyte survival ... LMP1 activates the IkappaB kinase complex and NF-kappaB through two cytoplasmic signaling domains that engage tumor necrosis factor receptor associated factor ( TRAF)1/2/3/5 or TRADD and RIP ... We now use cells lacking expression of TRAF2, TRAF5, TRAF6, IKKalpha, IKKbeta, IKKgamma, TAB2, IL-1 receptor associated kinase (IRAK)1, or IRAK4 to assess their roles in LMP1 mediated NF-kappaB activation ... Consistent with the importance of TRAF6, LMP1 induced NF-kappaB activation in HEK293 cells was inhibited by expression of dominant negative TAB2 and Ubc13 alleles ... Consistent with the importance of TRAF6, LMP1 induced NF-kappaB activation in HEK293 cells was inhibited by expression of dominant negative TAB2 and Ubc13 alleles ... These data extend a role for IKKalpha in IKKbeta regulation, identify an unusual IKKbeta dependent and IKKgamma independent NF-kappaB activation, and indicate that IRAK1 and TRAF6 are essential for LMP1 induced NF-kappaB activation ... These data extend a role for IKKalpha in IKKbeta regulation, identify an unusual IKKbeta dependent and IKKgamma independent NF-kappaB activation, and indicate that IRAK1 and TRAF6 are essential for LMP1 induced NF-kappaB activation ... These data extend a role for IKKalpha in IKKbeta regulation, identify an unusual IKKbeta dependent and IKKgamma independent NF-kappaB activation, and indicate that IRAK1 and TRAF6 are essential for LMP1 induced NF-kappaB activation
Wang et al., Mol Cell Biol 2004 : CD3/CD28 costimulation induced NF-kappaB activation is mediated by recruitment of protein kinase C-theta, Bcl10, and IkappaB kinase beta to the immunological synapse through CARMA1 ... This membrane association is essential for the function of CARMA1, since a mutant of CARMA1, CARMA1 ( L808P ), that is defective in the membrane association can not rescue CD3/CD28 costimulation induced NF-kappaB activation in JPM50.6 CARMA1-deficient T cells ... Consistently, expression of a mutant CARMA1, CARMA1 ( DeltaCD ), that can not associate with Bcl10 failed to restore CD3/CD28 costimulation induced NF-kappaB activation in JPM50.6 cells, whereas expression of Bcl10-CARMA ( DeltaCD ) fusion protein effectively restored this NF-kappaB activation ... Consistently, expression of a mutant CARMA1, CARMA1 ( DeltaCD ), that can not associate with Bcl10 failed to restore CD3/CD28 costimulation induced NF-kappaB activation in JPM50.6 cells, whereas expression of Bcl10-CARMA ( DeltaCD ) fusion protein effectively restored this NF-kappaB activation ... Together, these results indicate that CARMA1 mediates CD3/CD28 costimulation induced NF-kappaB activation by recruiting downstream signaling components into the immunological synapse ... Together, these results indicate that CARMA1 mediates CD3/CD28 costimulation induced NF-kappaB activation by recruiting downstream signaling components into the immunological synapse
Ishizuka et al., J Infect Dis 2003 (Picornaviridae Infections...) : RV-14 infection increased PAF-R expression and the activation of NF-kappaB and promoter-specific transcription factor 1
Tomsig et al., Biochem J 2004 : The introduction of calcium into HEK293 cells either through the activation of muscarinic cholinergic receptors or through the application of the ionophore A23187 was found to enhance TNF-alpha dependent activation of NF-kappaB
Misra et al., Circulation 2003 (Myocardial Infarction...) : These studies suggest that the cytoprotective effects of NF-kappaB are mediated , at least in part, by Bcl-2 or c-IAP1 ... These studies suggest that the cytoprotective effects of NF-kappaB are mediated , at least in part, by Bcl-2 or c-IAP1
Regamey et al., J Exp Med 2003 : Because TRIP is an inhibitor of nuclear factor (NF)-kappaB activation by tumor necrosis factor (TNF) , the effect of CYLD on NF-kappaB activation was investigated in HeLa cells ... Because TRIP is an inhibitor of nuclear factor (NF)-kappaB activation by tumor necrosis factor (TNF), the effect of CYLD on NF-kappaB activation was investigated in HeLa cells
Ackermann et al., Immunology 2004 : In studies using human intestinal epithelial cells ( HT29 ), multiple inhibitors of the transcription factor nuclear factor-kappaB (NF-kappaB) , including a dominant negative IkappaBalpha-serine mutant, inhibited both IL-4- and IFN dependent increases in pIgR expression ... In studies using human intestinal epithelial cells ( HT29 ), multiple inhibitors of the transcription factor nuclear factor-kappaB (NF-kappaB) , including a dominant negative IkappaBalpha-serine mutant, inhibited both IL-4- and IFN dependent increases in pIgR expression ... Under identical conditions, NF-kappaB inhibitors had no effect on cytokine dependent increases in expression of the transcription factor interferon regulatory factor-1 ... Finally, reporter gene studies with NF-kappaB enhancer elements indicated that IFN-gamma alone and IL-4 in combination with other cytokines activated NF-kappaB in HT29 cells ... Finally, reporter gene studies with NF-kappaB enhancer elements indicated that IFN-gamma alone and IL-4 in combination with other cytokines activated NF-kappaB in HT29 cells
Driessler et al., Clin Exp Immunol 2004 (Translocation, Genetic) : Molecular mechanisms of interleukin-10 mediated inhibition of NF-kappaB activity : a role for p50 ... We have recently shown that Interleukin-10 exerts its anti-inflammatory activity in part through the inhibition of NF-kappaB by blocking IkappaB kinase activity and by inhibiting NF-kappaB already found in the nucleus
Jensen et al., Environmental health : a global access science source 2003 : This suppression occurs at least at the level of IL-6 gene transcription and may be regulated by NF-kappaB
Xiong et al., J Biol Chem 2004 : Similarly, NO attenuates IL-1beta induced NF-kappaB activation
Afaq et al., Oncogene 2003 (Edema...) : Our data demonstrated that GTP inhibited UVB induced : ( i ) activation of NF-kappaB , ( ii ) activation of IKKalpha , and ( iii ) phosphorylation and degradation of IkappaBalpha
Verzola et al., J Am Soc Nephrol 2004 (Hyperglycemia) : At the same time, the expression of NF-kB induced antiapoptotic XIAP was reduced in HG-treated cells
Mamidipudi et al., J Biol Chem 2004 : We have previously shown that the activity of the interleukin-1 (IL-1) receptor associated kinase ( IRAK ) is required for nerve growth factor (NGF) induced activation of NF-kappaB and cell survival ( ( 2002 ) J. Biol. Chem. 277, 28010-28018 ) ... We have previously shown that the activity of the interleukin-1 (IL-1) receptor associated kinase ( IRAK ) is required for nerve growth factor (NGF) induced activation of NF-kappaB and cell survival ( ( 2002 ) J. Biol. Chem. 277, 28010-28018 ) ... Moreover, transfection of kinase-dead iota PKC blocked both NGF- and IL-1 induced IRAK activation and the activity of NF-kappaB ... Mutation of Thr66 to Ala impaired the autokinase activity of IRAK and reduced its association with iota PKC but not TRAF6, resulting in impaired NGF- as well as IL-1 induced NF-kappaB activation
Wu et al., Mol Endocrinol 2004 : Ang II stimulation induced MCP-1 mRNA expression as well as an increase in nuclear factor-kappaB (NF-kappaB) binding to the corresponding cis DNA element of the MCP-1 promoter region and a decrease in the cytosolic inhibitory protein-kappaB ( IkappaB ) protein level via AT(1) receptor stimulation, whereas stimulation of the AT(2) receptor decreased Ang II-induced MCP-1 expression, NF-kappaB DNA binding, and IkappaB degradation, suggesting that activation of the AT(2) receptor attenuated AT(1) receptor mediated MCP-1 expression via a decrease in NF-kappaB DNA binding and an increase in IkappaB stability ... Ang II stimulation induced MCP-1 mRNA expression as well as an increase in nuclear factor-kappaB (NF-kappaB) binding to the corresponding cis DNA element of the MCP-1 promoter region and a decrease in the cytosolic inhibitory protein-kappaB ( IkappaB ) protein level via AT(1) receptor stimulation, whereas stimulation of the AT(2) receptor decreased Ang II-induced MCP-1 expression, NF-kappaB DNA binding, and IkappaB degradation, suggesting that activation of the AT(2) receptor attenuated AT(1) receptor mediated MCP-1 expression via a decrease in NF-kappaB DNA binding and an increase in IkappaB stability ... Moreover, we demonstrated that AT(2) receptor stimulation attenuated TNFalpha mediated NF-kappaB activation and MCP-1 expression ... Moreover, we demonstrated that AT(2) receptor stimulation attenuated TNFalpha mediated NF-kappaB activation and MCP-1 expression ... Transfection of a dominant negative Src homology protein tyrosine phosphatase-1 mutant into vascular smooth muscle cells inhibited the AT(2) receptor mediated increase in IkappaB, leading to a significant increase in AT(1) receptor induced NF-kappaB activation and MCP-1 expression ... Ang II stimulation induced MCP-1 mRNA expression as well as an increase in nuclear factor-kappaB (NF-kappaB) binding to the corresponding cis DNA element of the MCP-1 promoter region and a decrease in the cytosolic inhibitory protein-kappaB ( IkappaB ) protein level via AT(1) receptor stimulation, whereas stimulation of the AT(2) receptor decreased Ang II-induced MCP-1 expression, NF-kappaB DNA binding, and IkappaB degradation, suggesting that activation of the AT(2) receptor attenuated AT(1) receptor mediated MCP-1 expression via a decrease in NF-kappaB DNA binding and an increase in IkappaB stability
Schölzke et al., Eur J Neurosci 2003 : Glutamate induced a rapid reduction of IkappaBalpha levels and nuclear translocation of the NF-kappaB subunit p65 ... Our data indicate that the Ca2+ dependent protease calpain is involved in the NF-kappaB activation in neurons in response to N-methyl-d-aspartate receptor occupancy by glutamate ... NF-kappaB activation by calpain may mediate the long-term effects of glutamate on neuron survival or memory formation
Chung et al., Infect Immun 2004 : However, in addition, hBD-2 induction by pathogenic bacteria in both oral and foreskin keratinocytes was blocked by inhibitors of NF-kappaB
Riedemann et al., FASEB J 2004 (MAP Kinase Signaling System...) : Production of IL-6 in neutrophils by LPS was NF-kappaB dependent ( but not on the presence of p50 ) and dependent on phosphorylation of p38-mitogen activated protein kinase ( MAPK ) as well as p44/p42 MAPK ( ERK1/2 ) but not on phosphorylation of c-Jun N-terminal kinases ( JNK1/2 )
Ma et al., J Immunol 2004 : Studies conducted to understand the regulation of AP-1 and NF-kappaB activation by JNK MAPK revealed that both DXM and SP600125 inhibited IL-12p40 gene transcription by inhibiting the activation of AP-1 and NF-kappaB transcription factors as revealed by luciferase reporter and gel mobility shift assays ... Taken together, our results suggest that DXM may inhibit IL-12p40 production in LPS stimulated human monocytic cells by down regulating the activation of JNK MAPK, the AP-1, and NF-kappaB transcription factors ... Taken together, our results suggest that DXM may inhibit IL-12p40 production in LPS stimulated human monocytic cells by down regulating the activation of JNK MAPK, the AP-1, and NF-kappaB transcription factors
Kowalski et al., J Immunol 2004 (Pseudomonas Infections) : Bacterial binding leads to CFTR dependent bacterial internalization, initiation of NF-kappaB nuclear translocation, cellular desquamation, and eventual apoptosis of the infected cells, all of which are critical for innate immune resistance to infection with this pathogen
Dehmer et al., J Neurochem 2004 (Disease Models, Animal...) : Because an increase in inhibitory protein-kappa-Balpha ( IkappaBalpha ) expression and inhibition of translocation of the nuclear factor kappaB (NFkappaB) subunit p65 to the nucleus in dopaminergic neurons, glial cells and astrocytes correlated with the protective effects of pioglitazone, our results suggest that pioglitazone sequentially acts through PPARgamma activation , IkappaBalpha induction, block of NFkappaB activation, iNOS induction and NO-mediated toxicity ... Because an increase in inhibitory protein-kappa-Balpha ( IkappaBalpha ) expression and inhibition of translocation of the nuclear factor kappaB (NFkappaB) subunit p65 to the nucleus in dopaminergic neurons, glial cells and astrocytes correlated with the protective effects of pioglitazone, our results suggest that pioglitazone sequentially acts through PPARgamma activation , IkappaBalpha induction, block of NFkappaB activation, iNOS induction and NO-mediated toxicity ... Because an increase in inhibitory protein-kappa-Balpha ( IkappaBalpha ) expression and inhibition of translocation of the nuclear factor kappaB (NFkappaB) subunit p65 to the nucleus in dopaminergic neurons, glial cells and astrocytes correlated with the protective effects of pioglitazone, our results suggest that pioglitazone sequentially acts through PPARgamma activation , IkappaBalpha induction, block of NFkappaB activation, iNOS induction and NO-mediated toxicity
Ryo et al., Mol Cell 2003 (Breast Neoplasms) : The transcription factor NF-kappaB is activated by the degradation of its inhibitor IkappaBalpha , resulting in its nuclear translocation
Luftig et al., Proc Natl Acad Sci U S A 2004 : Epstein-Barr virus latent infection membrane protein 1 TRAF binding site induces NIK/IKK alpha dependent noncanonical NF-kappaB activation ... LMP1 activates NF-kappaB , in part, by engaging tumor necrosis factor (TNF) receptor associated factors ( TRAFs ), which also mediate NF-kappaB activation from LTbetaR and CD40 ... LTbetaR and CD40 activation of p100/NF-kappaB2 is now known to be NIK/IKKalpha dependent and IKKbeta/IKKgamma independent ... Thus, LMP1 induces typical canonical IKKbeta/IKKgamma dependent, atypical canonical IKKbeta-dependent/IKKgamma independent, and noncanonical NIK/IKKalpha dependent NF-kappaB activations ; NIK/IKKalpha dependent NF-kappaB activation is principally mediated by the LMP1 TRAF binding site ... Thus, LMP1 induces typical canonical IKKbeta/IKKgamma dependent, atypical canonical IKKbeta-dependent/IKKgamma independent, and noncanonical NIK/IKKalpha dependent NF-kappaB activations ; NIK/IKKalpha dependent NF-kappaB activation is principally mediated by the LMP1 TRAF binding site
Valles et al., Alcohol Clin Exp Res 2003 : Stimulation of hepatocytes with IL-1beta caused a higher activation of IL-1 associated kinase, extracellular receptor activated kinases 1 and 2, and nuclear factor-kappaB (NF-kappaB) in hepatocytes from alcohol fed animals than from controls ... Our results suggest the participation of the extracellular signal regulated kinase ( ERK ) 1/2 pathway in ethanol induced NF-kappaB activation, because treatment with PD-98059, an ERK1/2 inhibitor, partially suppressed IL-1beta induced NF-kappaB expression
Giri et al., Apoptosis 1999 : In this report, we investigated the effect of TNF on activation of NF-kappa B , c-Jun N-terminal kinase (JNK), and apoptosis in vincristine-resistant human histiocytic lymphoma U937-VR cells ... TNF induced NF-kappa B activation , I kappa B alpha degradation, and nuclear translocation of p65 were all found to be highly suppressed in the U-937-VR cells ... NF-kappa B activation by LPS , H2O2, and okadaic acid was also suppressed ... Thus, overall these results indicate that vincristine resistance correlates with suppression of NF-kappa B activation, cytotoxicity, and caspase-3 activation but enhancement of JNK activation by TNF
Fujita et al., Oral Oncol 2004 (Carcinoma, Squamous Cell...) : Our results suggest that NF-kappaB activated at early stages in the okadaic acid treated SCC-25 cells stimulated the promoter activity of Fas receptor in the cells leading to the apoptotic death of these cells
Kellum et al., Am J Physiol Regul Integr Comp Physiol 2004 (Inflammation) : HCl significantly increased LPS induced NO release and NF-kappa B DNA binding at pH 7.0 but not at pH 6.5 ... HCl significantly increased LPS induced NO release and NF-kappa B DNA binding at pH 7.0 but not at pH 6.5 ... Lactic acid also inhibited LPS induced NF-kappa B DNA binding
Zhou et al., Nature 2004 (Lymphoma, B-Cell, Marginal Zone) : Bcl10 is essential for NF-kappaB activation by T- and B-cell receptors ... Bcl10 overexpression is sufficient to activate NF-kappaB , a process that requires the NF-kappaB essential modulator NEMO ( also known as IKK-gamma ), which is the regulatory subunit of the IkappaB kinase complex ... Notably, a mutant form of NEMO that can not be ubiquitinated inhibited Bcl10 induced NF-kappaB activation ... Paracaspase and a ubiquitin conjugating enzyme ( UBC13 ) were both required for Bcl10 induced NEMO ubiquitination and subsequent NF-kappaB activation ... Thus, the adaptor protein Bcl10 promotes activation of NF-kappaB transcription factors through paracaspase- and UBC13 dependent ubiquitination of NEMO
Yasui et al., Proc Natl Acad Sci U S A 2004 : LMP1TM1-2 enabled approximately 40 % of wild-type LMP1 cytoplasmic domain mediated NF-kappaB activation, whereas TM3-4 or TM5-6 assayed in parallel had almost no effect independent of LMP1TM1-2 ... Because FWLY ( 38-41 ) was essential for LMP1 mediated signal transduction, and LMP1 activation of NF-kappaB is essential for proliferating B lymphocyte survival, inhibition of LMP1FWLY ( 41 ) -mediated LMP1/LMP1 intermolecular interactions is an attractive therapeutic target
Yamamoto et al., Biochem Biophys Res Commun 2004 (Inflammation) : Involvement of NF-kappaB in TGF-beta mediated suppression of IL-4 signaling
Kapoor et al., Mol Cell Biol 2004 (Glioblastoma) : Distinct domains in the SHP-2 phosphatase differentially regulate epidermal growth factor receptor/NF-kappaB activation through Gab1 in glioblastoma cells ... Although previous studies suggest that EGFR can induce NF-kappaB , the mechanism of this activation remains unknown ... We also show that EGFR induced NF-kappaB activation is mediated by the PI3-kinase/Akt activation loop ... Interestingly, overexpression of either one of the two SH2 domain mutants of SHP-2, R32E or R138E, slightly reduced NF-kappaB activity relative to that of wild-type SHP-2, indicating that the SH2 domains of SHP-2 are required for EGFR induced NF-kappaB activation ... On the other hand, ectopic overexpression of either a Gab1 mutant incapable of binding to SHP-2 ( Y627F ) or a phosphatase-inactive SHP-2 mutant ( C459S ) caused a significant increase in NF-kappaB activity ... On the other hand, ectopic overexpression of either a Gab1 mutant incapable of binding to SHP-2 ( Y627F ) or a phosphatase-inactive SHP-2 mutant ( C459S ) caused a significant increase in NF-kappaB activity
Lewis et al., J Biol Chem 2004 : The activation of NF-kappaB by XIAP was dependent on the E3 ubiquitin ligase activity of the RING domain
Fu et al., J Biol Chem 2004 : Repression of NF-kappaB by IkappaBalpha ( sr ) also facilitates tumor necrosis factor alpha induced apoptosis in the cell ... We showed that p105 ( sr ), which no longer generates p50 and undergoes signal induced degradation, effectively inhibits all NF-kappaB activities
Sepulveda et al., J Immunol 2004 : In addition, human hs4 enhancer activity required Oct-2 and correlated with expression of Oct coactivator from B cells (OCA-B)
Imose et al., Liver Int 2003 (Liver Failure) : The inducible activation of NF-kappaB and constitutive activation of Akt regulate hepatocyte survival against TNF-alpha, which occurs independent of Bcl-2 families
Karalis et al., J Biol Chem 2004 : In a recent study in the pituitary corticotroph cell line AtT20, oxidative stress stimulated the activity of nuclear transcription factor B ( NF-kappaB ), whereas corticotropin releasing hormone (CRH) inhibited both the constitutive and the oxidative stress induced NF-kappaB DNA binding activity ... To further investigate the role of NF-kappaB on the CRH induced pituitary POMC gene activation, AtT20 cells were transiently transfected with a POMC-luciferase construct mutated at an NF-kappaB binding site ... In agreement with a previous report, CRH inhibited the constitutive NF-kappaB DNA binding activity in AtT20 cells, as shown by electrophoretic mobility-shift assay, as soon as within 15 min of treatment ... Our findings provide evidence that the regulation of corticotroph NF-kappaB activity by CRH is related to the activation of the pituitary POMC gene and, thus, may play an important role in stress response
Takada et al., J Biol Chem 2004 : We have identified two peptides from the p65 subunit of NF-kappaB ( P1 and P6 were from amino acid residues 271-282 and 525-537, respectively ) that, when linked with a PTD derived from the third helix sequence of antennapedia, inhibited tumor necrosis factor (TNF) induced NF-kappaB activation in vivo ... PTD-p65-P1 suppressed NF-kappaB activation induced by lipopolysaccharide, interleukin-1, okadaic acid, phorbol 12-myristate 13-acetate, H ( 2 ) O ( 2 ), and cigarette smoke condensate as well as that induced by TNF ... PTD-p65-P1 suppressed NF-kappaB activation induced by lipopolysaccharide, interleukin-1 , okadaic acid, phorbol 12-myristate 13-acetate, H ( 2 ) O ( 2 ), and cigarette smoke condensate as well as that induced by TNF ... PTD-p65-P1 had no effect on TNF induced inhibitory subunit of NF-kappaB ( IkappaBalpha ) phosphorylation, IkappaBalpha degradation, or IkappaBalpha kinase activation , but it blocked TNF induced p65 phosphorylation and nuclear translocation
Hellermann et al., J Allergy Clin Immunol 2004 : Similarly, pNHP ( 73-102 ) decreased TNF-alpha induced NFkappaB activation in NHBE cells
Habelhah et al., EMBO J 2004 : Ubiquitination and translocation of TRAF2 is required for activation of JNK but not of p38 or NF-kappaB ... Here, we demonstrate that TRAF2 ubiquitination is required for TNFalpha induced activation of JNK but not of p38 or NF-kappaB ... These findings demonstrate that the TRAF2 RING is required for Ubc13 dependent ubiquitination, resulting in translocation of TRAF2 to an insoluble fraction and activation of JNK, but not of p38 or NF-kappaB
Cinar et al., Biochem J 2004 (Prostatic Neoplasms) : We observed that activation of NF-kappaB by TNFalpha ( tumour necrosis factor alpha ) inhibited both basal and androgen stimulated PSA expression, and that this down-regulation occurred at the promoter level, as confirmed by the super-repressor IkappaBalpha ( S32A/S36A ), a dominant negative inhibitor of NF-kappaB ... We observed that activation of NF-kappaB by TNFalpha ( tumour necrosis factor alpha ) inhibited both basal and androgen stimulated PSA expression, and that this down-regulation occurred at the promoter level, as confirmed by the super-repressor IkappaBalpha ( S32A/S36A ), a dominant negative inhibitor of NF-kappaB ... We observed that activation of NF-kappaB by TNFalpha ( tumour necrosis factor alpha ) inhibited both basal and androgen stimulated PSA expression, and that this down-regulation occurred at the promoter level, as confirmed by the super-repressor IkappaBalpha ( S32A/S36A ), a dominant negative inhibitor of NF-kappaB
Bai et al., Biochem Biophys Res Commun 2004 : H ( 2 ) O ( 2 ) treatment stimulated phospholipase C-gamma1 ( PLC-gamma1 ), extracellular signal regulated kinase 1/2 ( ERK1/2 ), and NF-kappaB signaling but inhibited p38 mitogen activated protein kinase ( MAPK ) activation ... These data suggest that ERK and ERK dependent NF-kappaB activation is required for oxidative stress induced inhibition of osteoblastic differentiation in rabbit BMSC and calvarial osteoblasts
Polentarutti et al., Eur Cytokine Netw 2003 (Inflammation) : TIR8 did not activate NF-kB expression alone or in concert with IL-1R1
Hierholzer et al., Am J Physiol Gastrointest Liver Physiol 2004 (Enteritis...) : Selective iNOS inhibition in shocked animals reduced the binding activity of NF-kappaB and Stat3 and reduced mRNA levels of ICAM-1, IL-6, and G-CSF
Scholz et al., Int J Mol Med 2004 (Cytomegalovirus Infections) : Thrombin/PAR-1 mediated signaling stimulated PKC and NF-kappaB dependent IL-6 and IL-8 gene expression via phosphoinositide 3-kinase and further downstream via p42/44 and p38 MAPKs
Wright et al., Am J Respir Med 2003 (Lung Diseases) : Results of studies in in vitro and in vivo models of inflammation and malignancy have also suggested central roles for NF-kappaB in programmed cell death , or apoptosis
Yasuda et al., Cancer Sci 2004 (Colonic Neoplasms) : Luciferase reporter gene assay with pNF-kappaB-Luc revealed that NLK could suppress transcription activity of NF-kappaB in a kinase dependent manner
Yoshizumi et al., Exp Cell Res 2004 : Finally, TNF-alpha induced activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) activation and resultant intracellular adhesion molecule-1 ( ICAM-1 ) and vascular cell adhesion molecule-1 ( VCAM-1 ) expressions were inhibited by ebselen
Hu et al., Zhonghua Er Ke Za Zhi 2003 (Precursor Cell Lymphoblastic Leukemia-Lymphoma) : [ Regulation of NF-kappaB/P65 by MDM2 in acute lymphoblastic leukemia in childhood ] ... In the present study, the investigators hypothesized that MDM2 directly affected NF-kappaB expression and function in a P53 independent manner
Guo et al., Oncogene 2004 : Expression of ErbB2 enhances radiation induced NF-kappaB activation ... Stable transfection of dominant negative mutant IkappaB ( MCF-7/ErbB2/mIkappaB ) or treatment with anti-ErbB2 antibody, Herceptin, inhibited NF-kappaB activation and radiosensitized MCF-7/ErbB2 cells ... To identify specific genes affected by ErbB2 mediated NF-kappaB activation, a group of IR-responsive elements Cyclin B1, Cyclin D1, Bcl-2, Bcl/XL, BAD and BAX were evaluated ... These results thus provide evidence suggesting that overexpression of ErbB2 is able to enhance NF-kappaB response to IR, and that a specific prosurvival network downstream of NF-kappaB is triggered by treatments using anti-ErbB2 antibody combined with radiation
Lüschen et al., Exp Cell Res 2004 : Inhibition of p38 mitogen activated protein kinase reduces TNF induced activation of NF-kappaB , elicits caspase activity, and enhances cytotoxicity ... Inhibition of p38 mitogen activated protein kinase reduces TNF induced activation of NF-kappaB , elicits caspase activity, and enhances cytotoxicity
Vasudevan et al., Mol Cell Biol 2004 : These findings indicated that NF-kappa B activation was necessary and sufficient for inhibition of PTEN expression
Guo et al., Mol Cell Biol 2004 : We found that ( i ) p19(Arf) or p53 deficiency led to a significant increase in PI 3-kinase activity, which in turn upregulated RhoA and Rac1 activities ; ( ii ) deletion of p19Arf or p53 led to an increase in cell growth rate that was in part dependent on RhoA, Rac1, and Cdc42 activities ; ( iii ) p19(Arf) or p53 deficiency caused an enhancement of the growth related transcription factor NF-kappa B and cyclin D1 activities that are partly dependent on RhoA or Cdc42 but not on Rac1 ; ( iv ) forced expression of the activating mutants of Rac1, RhoA, or Cdc42 caused a hyperproliferative phenotype of the p19Arf ( -/- ) and p53 ( -/- ) cells and promoted transformation of both cells ; ( v ) RhoA appeared to contribute to p53 regulated cell proliferation by modulating cell cycle machinery, while hyperactivation of RhoA further suppressed a p53 independent apoptotic signal ; and ( vi ) multiple pathways regulated by RhoA, including that of Rho-kinase, were required for RhoA to fully promote the transformation of p53 ( -/- ) cells ... We found that ( i ) p19(Arf) or p53 deficiency led to a significant increase in PI 3-kinase activity, which in turn upregulated RhoA and Rac1 activities ; ( ii ) deletion of p19Arf or p53 led to an increase in cell growth rate that was in part dependent on RhoA, Rac1, and Cdc42 activities ; ( iii ) p19(Arf) or p53 deficiency caused an enhancement of the growth related transcription factor NF-kappa B and cyclin D1 activities that are partly dependent on RhoA or Cdc42 but not on Rac1 ; ( iv ) forced expression of the activating mutants of Rac1, RhoA, or Cdc42 caused a hyperproliferative phenotype of the p19Arf ( -/- ) and p53 ( -/- ) cells and promoted transformation of both cells ; ( v ) RhoA appeared to contribute to p53 regulated cell proliferation by modulating cell cycle machinery, while hyperactivation of RhoA further suppressed a p53 independent apoptotic signal ; and ( vi ) multiple pathways regulated by RhoA, including that of Rho-kinase, were required for RhoA to fully promote the transformation of p53 ( -/- ) cells
Chen et al., J Biomed Sci 2004 (MAP Kinase Signaling System) : A specific inhibitor of p38 MAPK ( SB203580 ) and an NF-kappaB inhibitor ( pyrrolidine dithiocarbamate ), but not an ERK inhibitor ( PD 98059 ), significantly inhibited neutrophil elastase induced IL-8 release and mRNA expression
Wu et al., Biochem J 2004 : Proteasome inhibitors up-regulate haem oxygenase-1 gene expression : requirement of p38 MAPK ( mitogen activated protein kinase ) activation but not of NF-kappaB ( nuclear factor kappaB ) inhibition
Towne et al., J Biol Chem 2004 (MAP Kinase Signaling System) : Recently, it was reported that IL-1F9 activated NF-kappaB through the orphan receptor IL-1 receptor (IL-1R) related protein 2 ( IL-1Rrp2 ) in Jurkat cells ( Debets, R., Timans, J. C., Homey, B., Zurawski, S., Sana, T. R., Lo, S., Wagner, J., Edwards, G., Clifford, T., Menon, S., Bazan, J. F., and Kastelein, R. A. ( 2001 ) J. Immunol. 167, 1440-1446 ) ... In this study, we demonstrate that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2 dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines ... In this study, we demonstrate that IL-1F6 and IL-1F8, in addition to IL-1F9, activate the pathway leading to NF-kappaB in an IL-1Rrp2 dependent manner in Jurkat cells as well as in multiple other human and mouse cell lines
Guillonneau et al., J Immunol 2004 (Acute Disease...) : The role of TNF related activation induced cytokine-receptor activating NF-kappa B interaction in acute allograft rejection and CD40L independent chronic allograft rejection
Sadikot et al., J Immunol 2004 (Pneumonia, Bacterial...) : p47phox deficiency impairs NF-kappa B activation and host defense in Pseudomonas pneumonia ... In vitro studies using bone marrow derived macrophages showed that Toll-like receptor 4 was necessary for NF-kappaB activation following treatment with P. aeruginosa
Nishiki et al., Am J Physiol Cell Physiol 2004 (MAP Kinase Signaling System) : G-CSF did not affect LPS induced activation of ERK, p38, JNK, and NF-kappa B , indicating that G-CSF affects the pathway downstream or independently of these signaling molecules ... G-CSF did not affect LPS induced activation of ERK, p38, JNK, and NF-kappa B , indicating that G-CSF affects the pathway downstream or independently of these signaling molecules
Park et al., Nephrol Dial Transplant 2004 : We investigated the role of NF-kappa B and AP-1 in tumour necrosis factor alpha (TNF-alpha) induced monocyte chemoattractant protein 1 ( MCP-1 ) production in cultured human glomerular endothelial cells ( HGEC ) ... ( i ) TNF-alpha increased NF-kappa B and AP-1 binding activity
Martín-Ventura et al., Stroke 2004 (Carotid Artery Diseases) : Nuclear factor-kappaB (NF-kappaB) is involved in the transcriptional regulation of the proapoptotic protein Fas ligand (FasL)
Hao et al., Apoptosis 2003 (Leukemia) : However, CEM cells showed a faster response to TRAIL induced NF-kappaB activation than K562 cells
Gotoh et al., Biochem Biophys Res Commun 2004 : Furthermore, the reduced expression of PKN1 by RNA interference ( RNAi ) down-regulated TRAF2 induced NF-kappaB activation in HEK293T cells ... Furthermore, the reduced expression of PKN1 by RNA interference ( RNAi ) down-regulated TRAF2 induced NF-kappaB activation in HEK293T cells
Kim et al., Biochem Biophys Res Commun 2004 : These results indicate that CN is required for full iNOS expression and the effective activation of NF-kappaB in RAW 264.7 and peritoneal macrophages
Darieva et al., J Leukoc Biol 2004 (Inflammation...) : Specific pharmacological inhibition of the PI-3K, c-jun-N-terminal kinase ( JNK ), and to a smaller extent, p38 MAPK but not extracellular regulated kinase (ERK), suppressed NF-kappaB dependent reporter gene transcription and MIP-2 and NO secretion in BCG induced RAW264.7 macrophages
Distler et al., Mol Pharmacol 2004 (Scleroderma, Systemic) : The study provides evidence for a role of NF-kappaB in the transcriptional regulation of the VEGF gene
Banning et al., Free Radic Biol Med 2004 : VCAM-1-inducibility was abrogated in SMCLOX but not in SMCPHGPx. Accordingly, NFkappaB-driven reporter gene activation by IL-1 was unaffected in SMCPHGPx but abolished in SMCLOX
Mamputu et al., Diabetes Obes Metab 2004 (MAP Kinase Signaling System) : Our results demonstrate the involvement of PKC, MAPK and NF-kappaB in AGE induced BREC proliferation and VEGF expression
Zhang et al., Cell Immunol 2003 : Investigation of the mechanism involved in MIP-1beta induction by IL-1beta showed that IL-1beta activated the nuclear factor kappa B (NF-kappaB) promoter in Huh7 cells ... In addition, caffeic acid phenethyl ester ( CAPE ), a specific inhibitor of the activation of NF-kappaB, not only abolished IL-1beta mediated NF-kappaB promoter activation , but also blocked IL-1beta induced MIP-1beta expression
Handa et al., J Pharmacol Exp Ther 2004 : Stimulation of RGM-1 cells with TNF-alpha resulted in an increase in intracellular oxidative stress, NF-kappaB translocation to the nuclei, and up-regulation of CINC-1 mRNA and protein, which was prevented by interfering with mitochondria dependent ROS production and NF-kappaB activation
Son et al., Endocrinology 2004 : Overexpression of the inhibitor of NF-kappaB ( called IkappaB ) blocked SAA3 promoter activity induced by TNF and by p65 in OV3121-1 cells
Hwang et al., Planta Med 2003 : Furthermore, sauchinone prevented LPS induced NF-kappaB activation, which is known to play a critical role in iNOS expression, assessed by a reporter assay under the control of NF-kappaB
Yun et al., Planta Med 2003 : Furthermore, panduratin A inhibited LPS induced NF-kappaB transcriptional activity in a dose dependent manner
Chio et al., Cell Signal 2004 : These results illustrate that PKA activation in macrophages is able to stimulate PKC and p38 MAPK, which lead to IKK dependent NF-kappaB activation and contribute to the induction of inducible nitric oxide synthase (iNOS) and IL-6 genes
Feinberg et al., J Biol Chem 2004 (Arteriosclerosis...) : Furthermore, we find that Smad3 prevents the cooperative induction of the iNOS promoter by C/EBP and NF-kappaB
Che et al., J Biol Chem 2004 (Lymphoma, B-Cell, Marginal Zone) : MALT1/paracaspase is a signaling component downstream of CARMA1 and mediates T cell receptor induced NF-kappaB activation ... Expression of a MALT1 deletion mutant containing only the N-terminal death domain and the two Ig-like domains completely blocked CD3/CD28 costimulation induced, but not tumor necrosis factor-alpha induced, NF-kappaB activation
Huang et al., J Biol Chem 2004 : Activation of NFkappaB requires the activity of IkappaB kinase , a kinase complex that contains two catalytic subunits, IKKalpha and IKKbeta, and a non-enzymatic regulatory subunit, IKKgamma ... To understand how NFkappaB activation is regulated at the IKKgamma level, we searched for IKKgamma interacting proteins by the yeast two-hybrid system ... ZNF216 also inhibited tumor necrosis factor (TNF)-, interleukin-1-, and Toll-like receptor 4-induced NFkappaB activation in a dose dependent manner ... The ZnF-A20 domain was essential for ZNF216 mediated inhibition of NFkappaB activation
Moon et al., J Cell Physiol 2004 : Furthermore, the transactivation of TNF-alpha stimulated NF-kappaB and AP-1 was inhibited by U0126 treatment ... Overexpression of RasN17 also abolished the TNF-alpha stimulated NF-kappaB and AP-1 activity
Homaidan et al., Mediators Inflamm 2003 (Enteritis) : IL-1 and ceramide, which have been shown to partially mediate IL-1 effects on IEC, activated NF-kappaB levels significantly
Gukovsky et al., Gastroenterology 2004 : CCK induced intracellular Ca ( 2+ ) mobilization, Ca ( 2+ ) influx, trypsinogen, and NF-kappa B activation were all diminished in pancreatic acini isolated from p110 gamma ( -/- ) mice
Holden et al., Eur J Biochem 2004 : ICAM-1 expression is highly NF-kappaB dependent in A549 cells
Inoue et al., J Immunol 2004 (MAP Kinase Signaling System) : CD40 ligation also resulted in the activation of NF-kappaB
Matsuyama et al., J Immunol 2004 : In this study, we report that the interaction of DDR1b with collagen up-regulates the production of IL-8, macrophage inflammatory protein-1alpha, and monocyte chemoattractant protein-1 in human macrophages in a p38 MAPK- and NF-kappaB dependent manner ... In this study, we report that the interaction of DDR1b with collagen up-regulates the production of IL-8, macrophage inflammatory protein-1alpha , and monocyte chemoattractant protein-1 in human macrophages in a p38 MAPK- and NF-kappaB dependent manner ... In this study, we report that the interaction of DDR1b with collagen up-regulates the production of IL-8 , macrophage inflammatory protein-1alpha, and monocyte chemoattractant protein-1 in human macrophages in a p38 MAPK- and NF-kappaB dependent manner ... p38 MAPK was critical for DDR1b mediated, increased NF-kappaB trans-activity, but not for IkappaB degradation or NF-kappaB nuclear translocation, suggesting a role for p38 MAPK in the modification of NF-kappaB
Yang et al., J Immunol 2004 : The zinc finger mutation C417R of I-kappa B kinase gamma impairs lipopolysaccharide- and TNF mediated NF-kappa B activation through inhibiting phosphorylation of the I-kappa B kinase beta activation loop ... Also, LPS- and TNF induced NF-kappaB transcription was inhibited by IKKgammaC417R ... Collectively, our results indicated that the zinc finger structure of IKKgamma plays a key role in LPS- and TNF induced NF-kappaB activation
Asehnoune et al., J Immunol 2004 : Involvement of reactive oxygen species in Toll-like receptor 4-dependent activation of NF-kappa B ... In addition, the mechanisms by which ROS modulate the transcriptional activity of NF-kappaB in response to Toll-like receptor 4 (TLR4) dependent signaling are not well characterized ... Pretreatment of neutrophils with the antioxidants N-acetylcysteine or alpha-tocopherol prevented LPS induced nuclear translocation of NF-kappaB
Zhou et al., J Immunol 2004 (Leukemia P388) : Similarly, dominant negative MAST205 mutants inhibit LPS stimulated IL-12 synthesis and NF-kappaB activation, but do not affect IL-1 or TNF-alpha signaling ... Similarly, dominant negative MAST205 mutants inhibit LPS stimulated IL-12 synthesis and NF-kappaB activation, but do not affect IL-1 or TNF-alpha signaling
Jin et al., Proc Natl Acad Sci U S A 2004 : Furthermore, the activation of NF-kappaB by TAB3 was blocked by the NF-kappaB inhibitor, SN50, and by expression of dominant negative forms of tumor necrosis factor alpha associated factor 6 and transforming growth factor beta activated kinase ... Furthermore, the activation of NF-kappaB by TAB3 was blocked by the NF-kappaB inhibitor, SN50, and by expression of dominant negative forms of tumor necrosis factor alpha associated factor 6 and transforming growth factor beta activated kinase
Baeza-Raja et al., Mol Biol Cell 2004 : Activation of p38 in C2C12 cells induced the activity of NF-kappaB, in a dual way : first, by reducing IkappaBalpha levels and inducing NF-kappaB-DNA binding activity and, second, by potentiating the transactivating activity of p65-NF-kappaB ... Finally, we show that interleukin (IL)-6 expression is induced in C2C12 differentiating myoblasts, in a p38- and NF-kappaB dependent manner
Rizzi et al., Eur J Immunol 2004 (HIV Infections) : Here we demonstrate that PTX-B inhibits Tat dependent NF-kappaB activation and HIV-1 LTR-transactivation in non-permissive epithelial HL3T1 cells in a phosphatidylinositol 3'-kinase dependent way ... Here we demonstrate that PTX-B inhibits Tat dependent NF-kappaB activation and HIV-1 LTR-transactivation in non-permissive epithelial HL3T1 cells in a phosphatidylinositol 3'-kinase dependent way
Litjens et al., Eur J Immunol 2004 (Psoriasis) : In addition, MMF inhibited LPS induced NF-kappaB activation in DC
Sadler et al., Anal Biochem 2004 : In response to diverse stimuli, the transcription factor NF-kappaB is activated by the IKK kinase complex containing two kinases ( IKKalpha and IKKbeta ) that phosphorylate IkappaB, an inhibitory protein of NF-kappaB
Dai et al., Arthritis Rheum 2004 (Arthritis, Rheumatoid) : Increased levels of NF-kappaB in the nucleus, phosphorylated IkappaB, and PI 3-kinase were confirmed in monocytes cocultured with PHA prestimulated T cells, and the levels were further increased by stimulation with IL-18
Ganchi et al., Mol Biol Cell 1992 : I kappa B/MAD-3 masks the nuclear localization signal of NF-kappa B p65 and requires the transactivation domain to inhibit NF-kappa B p65 DNA binding ... Results from in vivo expression studies performed with these NF-kappa B p65 mutants revealed the following : 1 ) I kappa B/MAD-3 completely inhibits NF-kappa B p65 dependent transcriptional activation mediated through the human immunodeficiency virus type 1 kappa B enhancer in human T lymphocytes, 2 ) the binding of I kappa B/MAD-3 to NF-kappa B p65 is sufficient to retarget NF-kappa B p65 from the nucleus to the cytoplasm, 3 ) selective deletion of the functional nuclear localization signal present in the Rel homology domain of NF-kappa B p65 disrupts its ability to engage I kappa B/MAD-3, and 4 ) the unique C-terminus of NF-kappa B p65 attenuates its own nuclear localization and contains sequences that are required for I kappa B-mediated inhibition of NF-kappa B p65 DNA binding activity ... Results from in vivo expression studies performed with these NF-kappa B p65 mutants revealed the following : 1 ) I kappa B/MAD-3 completely inhibits NF-kappa B p65 dependent transcriptional activation mediated through the human immunodeficiency virus type 1 kappa B enhancer in human T lymphocytes, 2 ) the binding of I kappa B/MAD-3 to NF-kappa B p65 is sufficient to retarget NF-kappa B p65 from the nucleus to the cytoplasm, 3 ) selective deletion of the functional nuclear localization signal present in the Rel homology domain of NF-kappa B p65 disrupts its ability to engage I kappa B/MAD-3, and 4 ) the unique C-terminus of NF-kappa B p65 attenuates its own nuclear localization and contains sequences that are required for I kappa B-mediated inhibition of NF-kappa B p65 DNA binding activity
Durán et al., Dev Cell 2004 (Bone Resorption) : Here we show that p62, like TRAF6, is upregulated during RANK-L induced osteoclastogenesis and that the genetic inactivation of p62 in mice leads to impaired osteoclastogenesis in vitro and in vivo, as well as inhibition of IKK activation and NF-kappa B nuclear translocation
Chandrasekar et al., J Biol Chem 2004 (Inflammation) : Treatment of HCMEC with IL-18 increases 1 ) NF-kappaB DNA binding activity ; 2 ) induces kappaB-driven luciferase activity ; 3 ) induces IL-1beta and TNF-alpha expression via NF-kappaB activation ; 4 ) inhibits antiapoptotic Bcl-2 and Bcl-X ( L ) ; 5 ) up-regulates proapoptotic Fas, Fas-L, and Bcl-X ( S ) expression ; 6 ) induces fas and Fas-L promoter activities via NF-kappaB activation ; 7 ) activates caspases-8, -3, -9, and BID ; 8 ) induces cytochrome c release into the cytoplasm ; 9 ) inhibits FLIP ; and 10 ) induces HCME cell death by apoptosis as seen by increased annexin V staining and increased levels of mono- and oligonucleosomal fragmented DNA
Gentry et al., J Biol Chem 2004 : Reciprocally, NRIF enhanced TRAF6 mediated activation of the c-Jun NH2-terminal kinase (JNK) by 3-fold, while only modestly increasing the stimulation of NF-kappaB
O'Mahony et al., J Biol Chem 2004 (Cell Transformation, Viral) : Human T-cell lymphotropic virus type 1 tax induction of biologically Active NF-kappaB requires IkappaB kinase-1 mediated phosphorylation of RelA/p65 ... Human T-cell lymphotropic virus type 1 tax induction of biologically Active NF-kappaB requires IkappaB kinase-1 mediated phosphorylation of RelA/p65 ... However, NF-kappaB induced by Tax in these IKK1 ( -/- ) cells was functionally impaired ... Furthermore, reconstitution of IKK1 ( -/- ) cells with kinase-proficient ( but not kinase-deficient ) forms of IKK1 restored the Tax induction of full NF-kappaB transactivation ... Together, these findings highlight distinct roles for the IKK1 and IKK2 kinases in the activation of NF-kappaB in response to HTLV-1 Tax
Zhao et al., J Am Coll Nutr 2004 : The effect of eicosapentaenoic acid ( EPA ), a major n-3 fatty acid in fish oil, on the lipopolysaccharide (LPS) induced expression of TNF-alpha and activation of NF-kappaB were investigated ... The effect of eicosapentaenoic acid ( EPA ), a major n-3 fatty acid in fish oil, on the lipopolysaccharide (LPS) induced expression of TNF-alpha and activation of NF-kappaB were investigated ... LPS induced NF-kappaB activation , translocation of p65 subunit to the nucleus, phosphorylation and degradation of IkappaB-alpha were partially prevented by EPA ... LPS induced NF-kappaB activation , translocation of p65 subunit to the nucleus, phosphorylation and degradation of IkappaB-alpha were partially prevented by EPA
Fan et al., Curr Med Chem 2004 : Further studies indicate that paclitaxel activates Ikappa B Kinase (IKK), which in turn causes degradation of Ikappa Balpha and activation of NF-kappa B , whereas glucocorticoids antagonize paclitaxel mediated NF-kappa B activation through induction of Ikappa Balpha synthesis
Lee et al., J Biol Chem 2004 : A saturated fatty acid, lauric acid, induced NFkappaB activation when TLR2 was co-transfected with TLR1 or TLR6 in 293T cells, but not when TLR1 , 2, 3, 5, 6, or 9 was transfected individually
Wu et al., J Biomed Sci 2004 : Glossogyne tenuifolia acts to inhibit inflammatory mediator production in a macrophage cell line by downregulating LPS induced NF-kappa B ... Both of these compounds inhibited LPS stimulated inflammatory mediator production and NF-kappa B activation
Tago et al., Life Sci 2004 : Hsp90 inhibitors attenuate effect of dexamethasone on activated NF-kappaB and AP-1 ... Both tumor necrosis factor-alpha activated NF-kappaB and phorbol ester activated AP-1 were inhibited by Dex and Hsp90 inhibitors alone ... These results suggest that Hsp90 inhibitor itself inhibits the activation of NF-kappaB and AP-1, however, impedes Dex induced inhibition of IL-1beta induction by attenuating Dex mediated activation of GR and inhibition of the proinflammatory transcription factors
Eichten et al., Virology 2004 : Expression of two insulin-like growth factor-1 binding proteins, IGFBP-2 and -5, is increased presumably in response to enhanced NF-kappaB activity in HPV-16 E7-expressing serum starved cells ... Expression of two insulin-like growth factor-1 binding proteins, IGFBP-2 and -5, is increased presumably in response to enhanced NF-kappaB activity in HPV-16 E7-expressing serum starved cells ... Expression of two insulin-like growth factor-1 binding proteins, IGFBP-2 and -5 , is increased presumably in response to enhanced NF-kappaB activity in HPV-16 E7-expressing serum starved cells
Guilbault et al., J Biol Chem 2004 : Instead, elevated RasGRP1 expression caused down-regulation of NF-kappaB and Bcl-x ( L ), which provide survival signals counter acting apoptosis induction by B cell receptor
Tegeder et al., J Neurosci 2004 (Disease Models, Animal...) : In cell culture experiments, S1627 inhibited interleukin (IL)-1beta stimulated nuclear translocation and DNA binding of NF-kappaB ... In cell culture experiments, S1627 inhibited interleukin (IL)-1beta stimulated nuclear translocation and DNA binding of NF-kappaB
Lan et al., Chin Med J (Engl) 2004 : NF-kappa B was activated by Ox-LDL persistently and rebounded in the early period ... Ox-LDL induced NF-kappa B activation in a dose dependent way ... Ox-LDL induced activation of NF-kappa B persistently ... NF-kappa B may be involved in the enhancement of TGF-beta 1 induced by Ox-LDL in rat mesangial cells
Zhang et al., Endocrinology 2004 : Overexpression of the nuclear factor-kappaB (NFkappaB) p50 and/or p65 proteins increased the transcriptional activity of the beta-casein and WAP promoters in HC11 cells, suggesting that the inhibitory effect of TNF on transcription of these genes is not mediated by NFkappaB
Luo et al., J Biol Chem 2004 : Modulation of nuclear binding activities at the IL-12 p40 NFkappaB half-site was induced by EKLF for down-regulation of IL-12 p40 transcription in activated RAW264.7 cells, but no effect of EKLF on NFkappaB activity was observed in resting RAW264.7 cells
Sitaraman et al., FASEB J 2004 (Colitis...) : In response to luminal leptin , model intestinal epithelia critically activate the NF-kappaB , a key signaling system to pro-inflammatory stimuli
Ryu et al., Infect Immun 2004 (Trichomonas Vaginitis) : Furthermore, an NF-kappaB inhibitor ( pyrrolidine dithiocarbamate ), a mitogen activated protein ( MAP ) kinase ( MEK ) inhibitor ( PD98059 ), and a p38 MAP kinase inhibitor ( SB203580 ) significantly suppressed IL-8 synthesis in neutrophils
Kim et al., Infect Immun 2004 (Enteritis...) : These cells do not signal through Toll-like receptor 4 (TLR4) or respond to bacterial lipopolysaccharide, but they express functional TLR5 and interleukin 1 (IL-1) receptors that signal the activation of NF-kappaB in response to bacterial flagellin or IL-1 stimulation ... Stable expression of dominant negative ( DN ) Nod1 in colon epithelial cells prevented IkappaB kinase and NF-kappaB activation in response to infection with enteroinvasive Escherichia coli
Dedeoglu et al., Int Immunol 2004 : Induction of activation induced cytidine deaminase gene expression by IL-4 and CD40 ligation is dependent on STAT6 and NFkappaB ... Induction of activation induced cytidine deaminase gene expression by IL-4 and CD40 ligation is dependent on STAT6 and NFkappaB ... IL-4 induced STAT6 binding to a site in the 5 ' upstream region of the AID gene, while CD40 ligation induced NFkappaB binding to two sites in that region ... IL-4 induced STAT6 binding to a site in the 5 ' upstream region of the AID gene, while CD40 ligation induced NFkappaB binding to two sites in that region ... IL-4 induced STAT6 binding to a site in the 5 ' upstream region of the AID gene, while CD40 ligation induced NFkappaB binding to two sites in that region
Xiong et al., Virology 2004 : Using a nuclear factor-kappaB (NF-kappaB) dependent reporter assay, it was shown that activation of NF-kappaB was moderately increased in the presence of expression of MyD88 , and further significantly increased by co-expression of HBV
Clark et al., Exp Cell Res 2004 (Leukemia, Promyelocytic, Acute) : This maturation response involves protein kinase C ( PKC ) signaling, activation of the transcription factor nuclear factor kappaB (NFkB) , and intracellular calcium and calpain activity
Sonnenberg et al., Obes Res 2004 (Metabolic Syndrome X...) : Elevated free fatty acid, glucose, and insulin levels enhance this NF-kappa B activation and further downstream modulate specific clinical manifestations of metabolic syndrome
Sun et al., Acta Anaesthesiol Scand 2004 : Endotoxin caused transient production of TNF-alpha and IL-6 and activation of NF-kappaB in the intestine at peak times of 1, 4 and 1 h, respectively ... Endotoxin caused transient production of TNF-alpha and IL-6 and activation of NF-kappaB in the intestine at peak times of 1, 4 and 1 h, respectively
Jafari et al., Respirology 2004 (Respiratory Distress Syndrome, Adult) : NAC blocked stretch induced NF-kappa B and AP-1 binding and inhibited IL-8 mRNA expression
Jiang et al., Proc Natl Acad Sci U S A 2004 : Toll-like receptor 3-mediated activation of NF-kappaB and IRF3 diverges at Toll-IL-1 receptor domain containing adapter inducing IFN-beta ... Recently, a novel Toll-IL-1 receptor ( TIR ) -containing adapter, TIR domain containing adapter inducing IFN-beta ( TRIF ), was shown to play a critical role in TLR3 mediated activation of NF-kappaB and IRF3 ... Interestingly, mutation of the TRAF6 binding site of TRIF only abolished its ability to activate NF-kappaB but not IRF3, suggesting that TLR3 mediated activation of NF-kappaB and IRF3 might bifurcate at TRIF ... In support of this finding, we showed that DN-TRAF6 and DN-TAK1 blocked poly ( I.C ) -induced NF-kappaB but not IRF3 activation
Werman et al., Proc Natl Acad Sci U S A 2004 (Inflammation) : Under conditions of IL-1 receptor blockade, intracellular overexpression of the precursor and propiece forms of IL-1alpha were sufficient to activate NF-kappaB and AP-1
Egan et al., Proc Natl Acad Sci U S A 2004 : IkappaB-kinasebeta dependent NF-kappaB activation provides radioprotection to the intestinal epithelium
Matsumori et al., Eur J Heart Fail 2004 (Cardiovirus Infections...) : We examined the effects of SUN C8079 on the transcriptional responses of NF-kappaB, on activation of NF-kappaB in electrophoretic mobility shift assay, and on the gene expressions of tumor necrosis factor (TNF)-alpha and iNOS ... We examined the effects of SUN C8079 on the transcriptional responses of NF-kappaB, on activation of NF-kappaB in electrophoretic mobility shift assay, and on the gene expressions of tumor necrosis factor (TNF)-alpha and iNOS
Fredericks et al., Protein Eng Des Sel 2004 : The ease of producing antibodies in the scFv-Fc format permitted rapid identification of four lead clones ( C10, C13, C14, C15 ) that inhibit NF-kappaB nuclear translocation induced by IL-1
Chendil et al., Oncogene 2004 (Prostatic Neoplasms) : It has been reported that curcumin inhibits TNF-alpha induced NFkappaB activity that is essential for Bcl-2 protein induction ... However, curcumin in combination with radiation treated showed inhibition of TNF-alpha mediated NFkappaB activity resulting in bcl-2 protein downregulation
Ichiyama et al., Naunyn Schmiedebergs Arch Pharmacol 2004 (Mucocutaneous Lymph Node Syndrome) : We examined whether or not IVIG inhibits TNF-alpha induced activation of transcription factor NF-kappaB , a factor that is essential for the expression of proinflammatory cytokines, in human monocytic U-937 cells ... The inhibitory effect of IVIG on NF-kappaB activation induced by TNF-alpha was evaluated by Western blotting and flow cytometry ... These findings suggest that IVIG inhibits TNF-alpha induced NF-kappaB activation in monocytes/macrophages, and blocks FcgammaRIII on the membranes of monocytes/macrophages
Kreydiyyeh et al., Pflugers Arch 2004 : The activation of NF-kappaB by IL-1beta was maximal at 20 min and declined thereafter ... The effects of IL-1beta on the pump and NF-kappaB were prevented by the COX inhibitor indomethacin
Shanmugam et al., Diabetes 2004 : Promoter deletion analyses and inhibition of transcription by NF-kappaB superrepressor and cAMP-responsive element binding ( CREB ) mutants confirmed the involvement of NF-kappaB and CREB transcription factors in high glucose induced COX-2 regulation
Lin et al., J Biol Chem 2004 (Inflammation...) : Cr3 induces inflammatory cytokine secretion by activating NF-kappaB and p38 mitogen activated protein kinase in a Ca2+ dependent manner, but influx by extra-cellular Ca2+ is not involved
Caposio et al., J Virol 2004 : Here we report that HCMV infection activates the upstream IkappaB kinase (IKK) complex and that its catalytic IKK2 subunit is required for HCMV induced NF-kappaB activation, as well as the replication of different HCMV strains
Dirsch et al., Eur J Nutr 2004 : This study addresses the question whether the garlic metabolites diallyldisulfide ( DADS ) and allylmercaptane ( AM ) influence the TNF-alpha induced activation of NF-kappaB and the NF-kappaB regulated endothelial gene product E-selectin in human umbilical endothelial cells ( HUVECs )
Deng et al., Mol Carcinog 2004 : beta-catenin has been recently found to interact with and inhibit nuclear factor kappa B (NF-kappaB) ... In this study, we investigated whether GSK-3beta/APC might regulate NF-kappaB activity through beta-catenin ... In this study, we investigated whether GSK-3beta/APC might regulate NF-kappaB activity through beta-catenin ... We found that inhibition of GSK-3beta suppressed NF-kappaB activity, whereas reexpression of APC restored NF-kappaB activity in APC mutated cells
Bunnell et al., Mol Interv 2002 (Lymphoma, B-Cell, Marginal Zone) : Two recent reports demonstrate that CARMA1 ( caspase recruitment domain containing membrane associated guanylate kinase protein-1 ) bridges the gap between PKCtheta and the IkappaB kinase (IKK) dependent activation of NF-kappaB in T cells
Watanabe et al., Proc Natl Acad Sci U S A 2004 (MAP Kinase Signaling System) : We show that NTHi and TNF-alpha , when present together, synergistically induce NF-kappaB activation via two distinct signaling pathways : NF-kappaB translocation dependent and -independent pathways ... The NF-kappaB translocation dependent pathway involves NF-kappaB inducing kinase-IkappaB kinase beta/gamma dependent phosphorylation and degradation of IkappaBalpha, whereas the NF-kappaB translocation independent pathway involves mitogen activated protein kinase ( MAPK ) /extracellular signal regulated kinase kinase kinase 1-dependent activation of MAPK kinase 3/6-p38 MAPK pathway
Müller et al., Cell Mol Biol Incl Cyto Enzymol 2003 : Differential involvement of ceramide in TNFalpha mediated activation of NF-kappaB in primary human keratinocytes and HaCaT keratinocytes ... It is well known that TNFalpha activates the transcription factor NF-kappaB , but there have been controversial reports on the role of ceramide in TNFalpha mediated NF-kappaB activation ... Here we show by different lines of experimental evidence that TNFalpha is a strong inducer of NF-kappaB in both cell types, whereas C2-ceramide failed to activate NF-kappaB in HaCaT keratinocytes in contrast to primary keratinocytes
Yu et al., Int Immunopharmacol 2004 : These results indicate that NF-kappaB plays a potential role in the induction of NO and TNF-alpha by oligochitosan in macrophages
Hong et al., Int Immunopharmacol 2004 (Disease Models, Animal...) : Furthermore, CQ reduced the expression of TLR9 and TLR4 mRNA and the activation of NFkappaB and AP-1 stimulated by CpG ODN and LPS in ANA-1 cells
Varro et al., Cancer Res 2004 (Neoplasm Invasiveness...) : The activation of NFkappaB was required for interleukin-8 and cyclooxygenase-2 activation but did not occur in cells responding to these paracrine mediators ... The activation of NFkappaB was required for interleukin-8 and cyclooxygenase-2 activation but did not occur in cells responding to these paracrine mediators
Marchisio et al., Int J Immunopathol Pharmacol 2004 : Apoptosis effected by TNF-alpha alone was suppressed by ATA and this event was paralleled by phosphorylation and nuclear translocation of Jak2, Stat2, Stat4 and NF-kB , along with inhibition of caspase activation
Jiang et al., J Biol Chem 2004 (Inflammation) : The present study examined the effect of angiotensin II on interleukin-1beta induced NF-kappaB activation and the subsequent expression of inducible NO synthase (iNOS) and vascular cell adhesion molecule-1 ( VCAM-1 ) in cultured rat vascular smooth muscle cells ... The inhibitory effect of angiotensin II on iNOS expression was associated with a down-regulation of the sustained activation of extracellular signal regulated kinase ( ERK ) and NF-kappaB by interleukin-1beta , whereas the effect on VCAM-1 was independent of ERK activation
Xu et al., J Biol Chem 2004 : The tumor necrosis factor receptor associated factor ( TRAF ) protein family members are critically involved in activation of NF-kappaB , JNK, and p38 activation triggered by tumor necrosis factor (TNF) receptor family members and toll/interleukin-1 receptor ( TIR ) -containing receptors
Soares et al., J Immunol 2004 (MAP Kinase Signaling System) : In addition, HO-1 inhibited the activation of NF-kappaB , a transcription factor required for TNF-alpha mediated up-regulation of these genes in EC
Korcok et al., J Bone Miner Res 2004 : Pretreatment of rabbit osteoclasts with osteoprotegerin inhibited translocation of NF-kappaB induced by RANKL but not by BzATP, establishing that the effects of BzATP are independent of RANKL signaling
Mitsuyama et al., J Bone Miner Res 2004 (Calcium Signaling) : It was demonstrated that Ca2+ signaling pathway involving calcineurin regulates IL-8 gene expression through activation of a transcription factor, NF-kappaB ... These results show that the Ca2+ signaling pathway involving calcineurin regulates IL-8 gene expression through activation of NF-kappaB in human osteoblast-like cells
Arai et al., Biochem Pharmacol 2004 : In the T cells stimulated with mitogenic lectin phytohemagglutinin ( PHA ) in conjunction with phorbol myristate acetate ( PMA ), 6-formylpterin suppressed the NF-kappaB dependent transcription, the production of cytokines ( IFN-gamma and IL-2 ) and the cell proliferation ... In the T cells stimulated with mitogenic lectin phytohemagglutinin ( PHA ) in conjunction with phorbol myristate acetate ( PMA ), 6-formylpterin suppressed the NF-kappaB dependent transcription, the production of cytokines ( IFN-gamma and IL-2 ) and the cell proliferation
Araujo et al., Clin Microbiol Infect 2004 : Whereas LPS induced a rapid increase in NF-kappa B activation, gemifloxacin alone did not
Barhoumi et al., Brain Res Mol Brain Res 2004 (Manganese Poisoning) : These data indicate that Mn augments LPS induced expression of NOS2 in C6 cells by increasing mitochondrial ROS and activation of NF-kappaB
Shindo et al., Biochem Biophys Res Commun 2004 (Ectodermal Dysplasia) : While IKK1/IKKalpha and IKK2/IKKbeta are required for EDAR induced NF-kappaB activation, they are dispensable for its ability to repress Lef-1/beta-catenin dependent transcription ... While IKK1/IKKalpha and IKK2/IKKbeta are required for EDAR induced NF-kappaB activation, they are dispensable for its ability to repress Lef-1/beta-catenin dependent transcription ... While IKK1/IKKalpha and IKK2/IKKbeta are required for EDAR induced NF-kappaB activation, they are dispensable for its ability to repress Lef-1/beta-catenin dependent transcription ... In contrast, NIK is not involved in EDAR induced NF-kappaB activation or Lef-1/beta-catenin transcriptional repression
Kim et al., Biochem Biophys Res Commun 2004 (Colorectal Neoplasms) : Furthermore, CDX2 was found to co-immunoprecipitate with the p65 subunit of NF-kappaB and to inhibit p65 induced NF-kappaB minimal promoter activity in colon cancer cells
Kobayashi-Sakamoto et al., Biochem Biophys Res Commun 2004 : NF-kappaB dependent induction of osteoprotegerin by Porphyromonas gingivalis in endothelial cells ... P. gingivalis induced NF-kappaB activation, and the induction of OPG in HMVEC by the pathogen was blocked by the inhibitors of NF-kappaB
Morceau et al., Biochem Pharmacol 2004 (Leukemia) : Regulation of glutathione S-transferase P1-1 gene expression by NF-kappaB in tumor necrosis factor alpha treated K562 leukemia cells ... Here we investigated the regulation of the GSTP1-1 gene expression in the K562 cell line by nuclear factor kappaB (NF-kappaB) and the pro-inflammatory cytokine tumor necrosis factor alpha (TNFalpha)
Kwak et al., Biochem Pharmacol 2004 (Bone Resorption) : M-CSF and RANKL activate the ERK, Akt, and NF-kappaB signal transduction pathways, and SCOH suppressed this activation ... M-CSF and RANKL activate the ERK, Akt, and NF-kappaB signal transduction pathways, and SCOH suppressed this activation
Yamanaka et al., Clin Cancer Res 2004 (Neoplasm Invasiveness...) : We examined the role of interleukin (IL)-1beta in activation of nuclear factor kappaB (NF-kappaB) and the biological function of activated NF-kappaB in gastric carcinoma cells ... We examined the role of interleukin (IL)-1beta in activation of nuclear factor kappaB (NF-kappaB) and the biological function of activated NF-kappaB in gastric carcinoma cells ... In vivo expression of IL-1beta and MMP-9 and activation of NF-kappaB in 10 surgically resected gastric carcinoma specimens were examined immunohistochemically ... In vivo expression of IL-1beta and MMP-9 and activation of NF-kappaB in 10 surgically resected gastric carcinoma specimens were examined immunohistochemically ... IL-1beta enhanced NF-kappaB activation, MMP-9 expression, and the invasive ability of GCTM-1 ... A NF-kappaB inhibitor, pyrrolidine dithiocarbamate, suppressed both MMP-9 expression and invasiveness of IL-1beta treated GCTM-1 cells ... The present results suggest that IL-1beta increases the invasive ability of carcinoma cells through activation of NF-kappaB and the resulting MMP-9 expression
St-Germain et al., Molecular cancer 2004 (Endometrial Neoplasms) : Inhibition of PI 3-K with Wortmannin and LY294002 blocked IkappaB phosphorylation, reduced NF-kappaB nuclear activity, reduced COX-2 expression and induced apoptosis
Shisler et al., J Virol 2004 : The vaccinia virus K1L gene product inhibits host NF-kappaB activation by preventing IkappaBalpha degradation ... Moreover, MVA/K1L gained the ability to repress artificially contrived and natural NF-kappaB regulated expression of a transfected luciferase and the cellular tumor necrosis factor gene, respectively
Choi et al., Free Radic Biol Med 2004 : The resistance involved IkappaB kinase (IKK) activation via iron induced reactive oxygen species formation, NF-kappaB activation by activated IKK , and c-FLIP expression by activated NF-kappaB
Afaq et al., Toxicol Appl Pharmacol 2004 (Edema...) : In search for novel markers of skin tumor promotion, we found that TPA application to mouse skin resulted, as an early event, in an increased expression of phosphatidyinositol 3-kinase (PI3K), phosphorylation of Akt at threonine308 and activation of nuclear factor kappa B (NF-kappaB)
Jang et al., Biochem Biophys Res Commun 2004 : Catalase also induced activation of NF-kappaB , PI3K, ERKs, p38s, or JNKs. Catalase induced COX-2 expression was abrogated by treatment of MG-132 ( a NF-kappaB inhibitor ) or LY294002 ( a PI3K inhibitor ), but not by treatment of PD98059 ( an ERK inhibitor ), SB203580 ( a p38 inhibitor ), or SP600125 ( a JNK inhibitor )
Makino et al., Oncogene 2004 : Here, we show that integrin linked kinase (ILK) , the crucial signal transducer in the integrin pathway, is involved in HER2/neu mediated activation of NF-kappaB
Paya et al., Proc Natl Acad Sci U S A 1992 : These observations indicate that during chronic HIV infection of U937 cells, continuous NF-kappa B ( p50/p65 ) translocation results in p105 promoter upregulation with subsequent cytosolic NF-kappa B accumulation, ready for further translocation
Ryoo et al., Cardiovasc Res 2004 : These data demonstrated that LDL stimulates SMCs to induce IL-8 production in dose- and time dependent manners at the transcription level and that the LDL signaling in hAoSMCs is conveyed via the generation of H2O2, the phosphorylation of p38 MAPK, the activation of AP-1 , and the participation of NF-kappaB
Podojil et al., J Biol Chem 2004 : We showed that CD86 stimulation increased the nuclear localization of NF-kappaB1 ( p50 ) and phosphorylated RelA ( p65 ) and increased Oct-2 expression and binding to the 3'-IgH enhancer, in a protein kinase C-dependent manner
Hong et al., Mol Cell Biol 2004 : In addition, overexpression of the p65 ( RelA ) subunit of NF-kappaB showed the same effect as TNF-alpha and inhibited Nur77 transactivation, suggesting the involvement of NF-kappaB activation in the observed gene repression ... The NF-kappaB inhibition of Nur77 transactivation was likely due to the competition of p65 for Nur77 binding with coactivators ... Finally, chromatin immunoprecipitation assays revealed that TNF-alpha treatment caused the recruitment of NF-kappaB to the promoter of the steroidogenic-enzyme p450c17 gene, supporting the hypothesis that the TNF-alpha mediated gene repression involves NF-kappaB inhibition of the transcriptional activity of Nur77 and other orphan nuclear receptors
Storz et al., Mol Cell Biol 2004 : Protein kinase Cdelta selectively regulates protein kinase D-dependent activation of NF-kappaB in oxidative stress signaling ... We also show that other PKCs, including PKCepsilon and PKCzeta, do not participate in PKD activation or NF-kappaB induction
Kataoka et al., Mol Cell Biol 2004 : FLIP ( p43 ) -induced NF-kappaB activation was profoundly blocked by the dominant negative TRAF2
Mabuchi et al., J Biol Chem 2004 (Ovarian Neoplasms) : Thus, high basal levels of phosphorylation of IkappaBalpha and activation of NFkappaB and less marked inhibition of the phosphorylation of IkappaBalpha and activation of NFkappaB by cisplatin seem to reduce the sensitivity of cells to cisplatin
Lo et al., Int J Cancer 2004 (Nasopharyngeal Neoplasms) : 2117-LMP1 could activate higher level of NF-kappaB activity in HEK 293 cells than B95.8-LMP1
Richmond et al., Novartis Found Symp 2004 (Cell Transformation, Neoplastic...) : We have shown that the constitutive activation of NF-kappaB in human melanoma tumours is accompanied by constitutive activation of the NF-kappaB inducing kinase (NIK) as well as the constitutive activation of AKT
Ludwig et al., Biochem Biophys Res Commun 2004 : EGCG did not influence TNF-alpha stimulated NF-kappaB activation
Tsunobuchi et al., Biochem Biophys Res Commun 2004 (MAP Kinase Signaling System) : In addition, TPA up-regulated Smad7 expression in the presence of NF-kappaB inhibitor TLCK
Hsieh et al., Biochem Biophys Res Commun 2004 : We show here that NF-kappaB which is a downstream regulator in RAGE mediated transduction pathways can be activated by addition of extracellular S100 proteins, and translocation of S100 proteins was inhibited by soluble RAGE
Meli et al., Ann N Y Acad Sci 2003 (Lymphoma, T-Cell) : Overall, these data suggest that NF-kappaB inhibition may restore the Fas-pathway in Fas-resistant NF-kappaB overexpressing tumors
Yasui et al., Ann N Y Acad Sci 2003 (Uterine Cervical Neoplasms) : We here demonstrate that sulindac inhibited TNF-alpha mediated NF-kappaB activation and greatly enhanced TNF-alpha induced apoptosis in human gastric MKN45 and cervical HeLa carcinoma cell lines
Andela et al., Molecular cancer 2004 (Neoplasm Metastasis) : Interestingly, NFkappaB blockade results in the reciprocal induction of retinoic acid receptors (RARs)
Kutuk et al., Cytokine 2004 : Aspirin inhibits TNFalpha- and IL-1 induced NF-kappaB activation and sensitizes HeLa cells to apoptosis ... TNFalpha- and IL-1 induced NF-kappaB activation pathways mainly involve the phosphorylation and degradation of IkappaBalpha by a signalsome complex followed by nuclear translocation of NF-kappaB and target gene expression ... Our studies reveal that acetylsalicylic acid ( aspirin ) prevents TNFalpha- and IL-1 induced NF-kappaB activation in a dose dependent manner through inhibition of phosphorylation and degradation of IkappaBalpha and IkappaBbeta
Kang et al., J Pharmacol Sci 2004 : Furthermore, curcumin markedly inhibited LPS induced nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1) DNA bindings
Sahni et al., Blood 2004 : Compared with free form, fibrinogen bound IL-1beta stimulated increased activation of endothelial cell nuclear factor kappaB (NF-kappaB) , monocyte chemoattractant protein-1 ( MCP-1 ) secretion, and nitric oxide ( NO ) synthesis
Suzuki et al., Infect Immun 2004 : However, endogenous tumor necrosis factor alpha- and Fas induced signals, extracellular signal regulated kinase kinase/mitogen activated protein kinases and I-kappa B alpha/nuclear factor-kappa B (NF-kappa B) were not required for caspase-3 dependent apoptosis
Ryan et al., Infect Immun 2004 : Reactive oxygen and nitrogen species differentially regulate Toll-like receptor 4-mediated activation of NF-kappa B and interleukin-8 expression ... These antioxidants also blocked LPS induced IL-8 promoter transactivation as well as the nuclear translocation of NF-kappa B
Lamkanfi et al., J Biol Chem 2004 (MAP Kinase Signaling System) : In contrast to pro-IL-1beta maturation, caspase-1 induced NF-kappaB activation is not inhibited by the virus derived caspase-1 inhibitor cytokine response modifier A and is equally induced by the enzymatically inactive caspase-1 C285A mutant ... We demonstrate that caspase-1 interacts with RIP2 and that dominant negative forms of RIP2 and IkappaB kinase complex-beta inhibit caspase-1 mediated NF-kappaB activation ... We demonstrate that caspase-1 interacts with RIP2 and that dominant negative forms of RIP2 and IkappaB kinase complex-beta inhibit caspase-1 mediated NF-kappaB activation ... We demonstrate that caspase-1 interacts with RIP2 and that dominant negative forms of RIP2 and IkappaB kinase complex-beta inhibit caspase-1 mediated NF-kappaB activation ... These data demonstrate that caspase-1 contributes to inflammation by two distinct pathways : proteolysis of pro-IL-1beta, and RIP2 dependent activation of NF-kappaB and p38 MAPK mediated by the caspase recruitment domain
Butcher et al., J Pharmacol Exp Ther 2004 : In fact, MT-KO cells had higher nuclear levels of NF-KappaB p65 than did MT-WT cells, despite a lower cellular NF-KappaB level and function, suggesting that metallothionein mediated the specific activity of NF-KappaB ... These data support the hypothesis that metallothionein positively regulates the cellular level and activity of NF-KappaB
Matsumura et al., J Leukoc Biol 2004 : TLR2 promoter activity and nuclear factor (NF)-kappaB activation by IL-1alpha were inhibited by TGF-beta treatment ... Pretreatment with TGF-beta strongly suppressed IL-1alpha induced TLR2 promoter activity and NF-kappaB activation, which was consistent with the down-regulation of type I IL-1 receptor ( IL-1RI ) mRNA expression
de Rossi et al., Anesth Analg 2004 : In this study, we investigated the effect of xenon and isoflurane on the lipopolysaccharide (LPS) induced activation of the nuclear transcription factor (NF)-kappaB and production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 in vitro ... LPS induced production of TNF-alpha and IL-6 as well as activation of NF-kappaB were significantly increased in the presence of xenon compared with controls ... LPS induced production of TNF-alpha and IL-6 as well as activation of NF-kappaB were significantly increased in the presence of xenon compared with controls ... LPS induced production of TNF-alpha and IL-6 as well as activation of NF-kappaB were significantly increased in the presence of xenon compared with controls ... This study has shown that monocytes respond to lipopolysaccharide (LPS) in the presence of xenon with an increased activation of nuclear transcription factor (NF)-kappaB, whereas isoflurane inhibits LPS induced activation of NF-kappaB
Collet et al., Dev Comp Immunol 2004 : Pyrrolidine dithiocarbamate ( PDTC ), an NF-kappaB inhibitor, significantly blocked poly I:C induced luciferase accumulation in RTG-P1 at intermediate doses ( 1-10 microM ), suggesting that Mx1 induction through the IFN signalling pathway is NF-kappaB dependent in fish
Fass et al., Brain Res 2004 : To determine whether different types of NO donors exert differential toxicity in a cholinergic neuronal model, we measured cell viability markers, indicators of NOx formation, levels of intracellular reduced glutathione ( GSH ), protein nitrosothiols, and the activation of the transcription factor NF-kappaB in a mouse medial septal cholinergic cell line ( clone SN56 ) following exposure to the NO donors S-nitroso-N-acetyl-dl-penicillamine ( SNAP ), 3-morpholinosydnonimine ( SIN-1 ), or sodium nitroprusside ( SNP ) ... SNAP and SNP, but not SIN-1, activated the transcription factor NF-kappaB , as indicated by increases in p65 nuclear immunoreactivity
Foryst-Ludwig et al., Biochem Biophys Res Commun 2004 (Helicobacter Infections) : Here we report that H. pylori induced NF-kappaB activation and the subsequent release of interleukin 8 (IL-8) are inhibited by curcumin ( diferuloylmethane ), a yellow pigment in turmeric ( Curcuma longa L. )
Rao et al., J Biol Chem 2004 (Breast Neoplasms...) : hCG treatment prevented the tumor necrosis factor dependent NF-kappaB and AP-1 activation, which paralleled a decrease in the phosphorylation and degradation of IkappaBalpha
Lin et al., J Biol Chem 2004 (Breast Neoplasms) : Cyr61 expression confers resistance to apoptosis in breast cancer MCF-7 cells by a mechanism of NF-kappaB dependent XIAP up-regulation ... Function neutralizing antibodies to alpha ( v ) beta ( 3 ) and alpha ( v ) beta(5) could inhibit Cyr61 mediated NF-kappaB activation as well as XIAP expression ... Function neutralizing antibodies to alpha ( v ) beta ( 3 ) and alpha ( v ) beta(5) could inhibit Cyr61 mediated NF-kappaB activation as well as XIAP expression
Aleyasin et al., J Neurosci 2004 : NF-kappaB inhibition also reduced p53 transcripts and p53 activity as measured by the p53-inducible messages, Puma and Noxa, implicating the p53 tumor suppressor in the mechanism of NF-kappaB mediated neuronal death ... Importantly, p53 expression still induces death in the presence of NF-kappaB inhibition, indicating that p53 acts downstream of NF-kappaB
Condon et al., Proc Natl Acad Sci U S A 2004 : Expression of IL-1beta in AF macrophages and activation of NF-kappaB in the maternal uterus increased with the gestational increase in SP-A ... We propose that augmented production of SP-A by the fetal lung near term causes activation and migration of fetal AF macrophages to the maternal uterus, where increased production of IL-1beta activates NF-kappaB , leading to labor
Matsumura et al., Biochem Biophys Res Commun 2004 : We report identification of a TIFA related protein, TIFAB, that inhibits TIFA mediated activation of NFkappaB
Kim et al., Biochem Biophys Res Commun 2004 (MAP Kinase Signaling System) : Furthermore, IGF-II remarkably increased the DNA binding activities of NF-kappaB and AP-1, and the IL-8 promoter activity
Cahir-McFarland et al., J Virol 2004 : The role of NF-kappa B in LMP1 and overall EBV latency III transcriptional effects was investigated by treating LCLs with BAY11-7082 ( BAY11 )
Li-Weber et al., Eur J Immunol 2004 : In this study, we show that the IL-4 promoter is synergistically activated by NF-kappa B , NF-AT and NF-IL6 at the NF-kappa B/NF-AT/NF-IL6 composite sites ... Inhibition of NF-kappa B nuclear translocation in living cells blocked binding of NF-kappa B to the IL-4 promoter ... The data provide first evidence that NF-kappa B is directly involved in IL-4 transcription
Hübinger et al., Exp Hematol 2004 (Lymphoma, Large B-Cell, Diffuse) : Furthermore, we showed that CD30 regulated expression of cIAP1 and cIAP2 was mediated by NFkappaB ... Furthermore, we showed that CD30 regulated expression of cIAP1 and cIAP2 was mediated by NFkappaB
Guo et al., World J Gastroenterol 2004 (Colitis, Ulcerative...) : This truncation mutation is responsible for the inability to activate nuclear factor (NF)-kappaB in response to bacterial lipopolysaccharide (LPS)
Campbell et al., Mol Cell 2004 (Bone Neoplasms) : Furthermore, UV-C and daunorubicin inhibit TNF induced NF-kappa B transactivation, indicating that this is a dominant effect
Kim et al., Circ J 2004 (Carotid Stenosis) : TWEAK induced activation of MMP-9 was mediated by activation of NF-kappaB
Shin et al., Biol Pharm Bull 2004 : Furthermore, KSM inhibited the LPS induced DNA binding activity of nuclear factor-kappaB (NF-kappaB) , which was associated with prevention of the inhibitor kappaB degradation ... These results suggest that the methanol extract of K. scoparia inhibits LPS induced iNOS and COX-2 expression by blocking NF-kappaB activation ... These results suggest that the methanol extract of K. scoparia inhibits LPS induced iNOS and COX-2 expression by blocking NF-kappaB activation
Radoja et al., Mol Cell Biol 2004 (Epidermolysis Bullosa) : We found that C/EBP-beta and AP-1 induced, while retinoic acid, glucocorticoid receptors, and NF-kappaB suppressed , the K15 promoter, along with other keratin gene promoters ... We found that C/EBP-beta and AP-1 induced, while retinoic acid, glucocorticoid receptors, and NF-kappaB suppressed , the K15 promoter, along with other keratin gene promoters
Goetz et al., Dev Comp Immunol 2004 : TNF transcripts are expressed in both mammals and fish with similar kinetics, and the involvement of NF-kappaB in TNF expression in fish suggests that transcriptional regulation may be similar within vertebrates
Chang et al., Ann Thorac Surg 2004 (Shock, Septic) : Nuclear factor kappaB (NF-kappaB) plays a central role in the regulation of IL-1beta and subsequent IL-1beta dependent inflammatory processes ... Our results suggest that elevated activation of NF-kappaB , at least in part, contributes to the dysregulated expression of IL-1beta and iNOS in the lungs of senescent animals ... Our results suggest that elevated activation of NF-kappaB , at least in part, contributes to the dysregulated expression of IL-1beta and iNOS in the lungs of senescent animals
Auch et al., FEBS Lett 2004 : This study delineates a novel role of dsRNA in inducing the expression of iNOS through dsRNA activated protein kinase ( PKR ) -mediated activation of NF-kappaB and p38 mediated activation of C/EBPbeta in human astroglia that may participate in virus induced neurological abnormalities
Monaco et al., Proc Natl Acad Sci U S A 2004 (Arteriosclerosis...) : We found that NF-kappa B is activated in these cells and that this activity involves p65 , p50, and c-Rel but not p52 or RelB ... We found that NF-kappa B is activated in these cells and that this activity involves p65, p50, and c-Rel but not p52 or RelB ... We found that NF-kappa B is activated in these cells and that this activity involves p65, p50, and c-Rel but not p52 or RelB ... We found that NF-kappa B is activated in these cells and that this activity involves p65, p50, and c-Rel but not p52 or RelB ... This NF-kappa B activation can be blocked by overexpression of I kappa B alpha or dominant negative I kappa B kinase (IKK)-2 but not dominant negative IKK-1 or NF-kappa B-inducing kinase, resulting in selective inhibition of inflammatory cytokines ( tumor necrosis factor alpha, IL-6, and IL-8 ), tissue factor, and matrix metalloproteinases without affecting the antiinflammatory cytokine IL-10 or tissue inhibitor of matrix metalloproteinases ... This NF-kappa B activation can be blocked by overexpression of I kappa B alpha or dominant negative I kappa B kinase (IKK)-2 but not dominant negative IKK-1 or NF-kappa B-inducing kinase, resulting in selective inhibition of inflammatory cytokines ( tumor necrosis factor alpha, IL-6, and IL-8 ), tissue factor, and matrix metalloproteinases without affecting the antiinflammatory cytokine IL-10 or tissue inhibitor of matrix metalloproteinases ... This NF-kappa B activation can be blocked by overexpression of I kappa B alpha or dominant negative I kappa B kinase (IKK)-2 but not dominant negative IKK-1 or NF-kappa B-inducing kinase, resulting in selective inhibition of inflammatory cytokines ( tumor necrosis factor alpha, IL-6, and IL-8 ), tissue factor, and matrix metalloproteinases without affecting the antiinflammatory cytokine IL-10 or tissue inhibitor of matrix metalloproteinases
Meylan et al., Nat Immunol 2004 : Analysis of Trif-deficient mice has shown that TLR3 dependent activation of the transcription factor NF-kappa B by the TLR3 ligand double stranded RNA is Trif dependent ... In the absence of RIP1, TLR3 mediated signals activating NF-kappa B, but not the kinase JNK or interferon-beta, were abolished, suggesting that RIP1 mediates Trif induced NF-kappa B activation
Zhou et al., Acta Pharmacol Sin 2004 (Leukemia, Myeloid) : In a dose related fashion, alpha-pinene inhibits the nuclear translocation of NF-kappa B induced by LPS in THP-1 cells, and this effect is partly due to the upregulation of I kappa B alpha expression
Parker et al., J Immunol 2004 (MAP Kinase Signaling System) : TLR2 activation caused slower induction of the NF-kappa B and mitogen activated protein kinase signaling pathways and yet a much enhanced and prolonged macrophage-inflammatory protein 1 alpha ( CC chemokine ligand 3 ) protein production, when compared with TLR4 stimulation
Arnold et al., Neuroimmunomodulation 2004 (Lymphoma) : Western blot analysis detected an increase in Bcl-2 while the levels of nuclear factor kappa B (NFkappaB) remained unchanged in GHo cells
Lin et al., J Biomed Sci 2004 : Furthermore, IL-1beta induced activation of nuclear factor-kappaB (NF-kappaB) was inversely correlated with the degradation of IkappaB-alpha in HTSMCs. IL-1beta induced COX-2 expression and PGE ( 2 ) synthesis were inhibited by the NF-kappaB inhibitor pyrrolidinedithiocarbamate ... Furthermore, IL-1beta induced activation of nuclear factor-kappaB (NF-kappaB) was inversely correlated with the degradation of IkappaB-alpha in HTSMCs. IL-1beta induced COX-2 expression and PGE ( 2 ) synthesis were inhibited by the NF-kappaB inhibitor pyrrolidinedithiocarbamate
Bottero et al., Mol Diagn 2003 : Kinetic experiments in HeLa cells show that TNF stimulation first induced NF-kappa B DNA binding within 30 minutes, followed by I kappa B-alpha gene transcription 30 minutes later ... Removal of TNF after stimulation resulted in a faster decrease in both NF-kappa B DNA binding activity and I kappa B-alpha mRNA levels
Neznanov et al., DNA Cell Biol 2004 : These two PTP regulate NFkappaB activity differently ; SHP-1 inhibits and SHP-2 stimulates NFkappaB activation
Zapolska-Downar et al., J Nutr Biochem 2004 : Butyrate inhibited TNFalpha induced activation of nuclear factor-kappaB (NF-kappaB) in HUVEC
Philpott et al., Proc Natl Acad Sci U S A 2004 : We propose that integrin mediated PI3K induction of NF-kappaB activates an autocrine TNF-alpha pathway required for DC maturation in response to Ad
Telleria et al., J Mol Endocrinol 2004 : Furthermore, NFkappaB DNA binding activity was enhanced by prolactin in luteinized granulosa cells ... In a second functional study, overexpression of NFkappaB/p65 in luteal cells resulted in inhibition of 20alpha-hydroxysteroid dehydrogenase ( 20alphaHSD ) promoter activity as well as endogenous 20alphaHSD mRNA expression ... In summary, we have shown that : ( i ) NFkappaB is expressed within the CL, primary luteinized granulosa cells, and a rat luteal cell line ; ( ii ) NFkappaB activation within the CL is developmentally regulated in pregnancy, depends on the age of the gland, and can be upregulated by prolactin ; ( iii ) inhibition of NFkappaB/p65 binding to an NFkappaB DNA consensus sequence correlates with induction of cell death in ovarian luteinized granulosa cells ; and ( iv ) overexpression of NFkappaB in luteal cells inhibits 20alphaHSD gene expression
Ravi et al., Drug Resist Updat 2004 (Neoplasms) : NF-kappaB may be inhibited by targeting either the apical signaling proteins responsible for its activation in specific types of cancer, the downstream kinases ( IkappaB kinase and casein kinase 2 ) at which NF-kappaB activating signaling pathways converge, the proteasome mediated degradation of the inhibitor of kappaB ( IkappaB ) proteins, or the transcriptional activity of Rel proteins
Mazighi et al., Am J Physiol Heart Circ Physiol 2004 (Hyperplasia...) : In LPS stimulated rat SMCs, 1 ng/ml recombinant murine IL-10 ( mIL-10 ) reduced I-kappaBalpha and I-kappaBbeta degradation, NF-kappaB activation, as well as the expression of the NF-kappaB dependent gene IL-6 by 32 %, 31 %, 75 %, and 19 %, respectively ( P < 0.05 for all )
Barsness et al., Am J Physiol Regul Integr Comp Physiol 2004 (Acute Disease...) : Hemorrhage induced nuclear factor (NF)-kappaB activation was independent of functional TLR-4, whereas endotoxin induced activation of NF-kappaB requires a functional TLR-4 for full response
Yeh et al., J Biol Chem 2004 : Previous findings that protein phosphatase 4 (PP4) , a nuclear serine/threonine phosphatase, directly interacts with and activates NF-kappaB led us to examine the phosphorylation status of NF-kappaB p65
Bohuslav et al., J Biol Chem 2004 : p53 induces NF-kappaB activation by an IkappaB kinase independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1 ... Here we describe the novel molecular mechanism through which p53 and DNA damaging agents activate NF-kappaB ... NF-kappaB induction by p53 does not occur through classical activation of the IkappaB kinases and degradation of IkappaBalpha
Chen et al., J Biol Chem 2004 (Crohn Disease) : In this study, we determined that mitogen activated protein kinase kinase transforming growth factor ( TGF)-beta activated kinase 1 (TAK1 ) interacts with Nod2 and is required for Nod2 mediated NF-kappaB activation ... In this study, we determined that mitogen activated protein kinase kinase transforming growth factor ( TGF)-beta activated kinase 1 (TAK1 ) interacts with Nod2 and is required for Nod2 mediated NF-kappaB activation ... Nod2 , acting in a reciprocal manner, inhibited TAK1 induced NF-kappaB activation in RICK-deficient embryonic fibroblasts ... Nod2, acting in a reciprocal manner, inhibited TAK1 induced NF-kappaB activation in RICK-deficient embryonic fibroblasts ... Nod2 appears to interact with TAK1 through its LRR region to exert its inhibitory effect on TAK1 induced NF-kappaB activation ... Further, wild-type LRR more effectively suppressed NF-kappaB activation induced by TAK1 than LRR with a 3020insC mutation
Mori et al., J Virol 2004 (Disease Models, Animal...) : Although the viral protein Tax is an activator of NF-kappaB and AP-1, FR901228 induced apoptosis was not associated with reduced expression of Tax
Hamilton et al., J Mol Cell Cardiol 2004 (Anoxia) : Electromobility shift assays revealed activation of HSF1 by 2 to 3 hr, and NF kappa B activation by 15 min. HSP72 induction via HSF1 activation was confirmed using transcription factor decoys containing the heat shock element, which prevented the estrogen related HSP72 induction
Jefferies et al., Immunol Lett 2004 : Further investigation into the role of Btk in LPS signalling has directly implicated Btk downstream of TLR4, both with respect to p38 MAPK activation and activation of the transcription factor NFkappaB ... Further investigation into the role of Btk in LPS signalling has directly implicated Btk downstream of TLR4, both with respect to p38 MAPK activation and activation of the transcription factor NFkappaB ... In fact Btk is activated by LPS and has been shown to directly bind TLR4 and the key proximal signalling proteins involved in LPS induced NFkappaB activation, MyD88, Mal and IRAK-1
Kim et al., Biochem Pharmacol 2004 (Bone Resorption) : RANKL activated the ERK, Akt, and NF-kappaB signal transduction pathways in osteoclast precursor cells, and tanshinone IIA suppressed this activation
Hardy et al., J Biol Chem 2004 : When overexpressed, Irak2a and Irak2b potentiated NF-kappaB activation by lipopolysaccharide
Scharschmidt et al., Mol Cell Biol 2004 : Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling
Eisele et al., J Cardiovasc Pharmacol 2004 : LDL induced activation of the transcription factor NF-kappaB was inhibited by ASA , and ferritin protein was increased when endothelial cells were incubated with this drug ... LDL induced activation of the transcription factor NF-kappaB was inhibited by ASA, and ferritin protein was increased when endothelial cells were incubated with this drug
Müerköster et al., Med Klin (Munich) 2004 (Carcinoma, Pancreatic Ductal...) : The autocrine secretion of IL-1beta contributes to a constitutively increased NF-kappaB activity in PDAC cells along with a chemoresistant phenotype
Matthews et al., Nucleic Acids Res 1992 : Thioredoxin regulates the DNA binding activity of NF-kappa B by reduction of a disulphide bond involving cysteine 62
Zafiriou et al., Kidney Int 2004 (Fibrosis...) : LDL induced increases in MCP-1 and TGF-beta1 were independent of nuclear factor-kappaB (NF-kappaB) transcriptional activity ... LDL induced increases in MCP-1 and TGF-beta1 were independent of nuclear factor-kappaB (NF-kappaB) transcriptional activity
Zoja et al., Curr Opin Nephrol Hypertens 2004 (Disease Progression...) : Albumin endocytosis in proximal tubular cells triggers events that include protein kinase C-dependent generation of reactive oxygen species, nuclear translocation of NF-kappaB , and activation of mitogen activated protein kinase
Russo et al., Cell Signal 2004 : Despite similar 125I-IL-1beta binding, HT-29 cells displayed no IRAK degradation, a 75 % reduction of IKK activity, and decreased IkappaBalpha phosphorylation, NF-kappaB DNA binding activity and IL-8 mRNA accumulation in response to IL-1beta compared to Caco-2 cells ... Selective activation of NF-kappaB pathway by adenoviral delivery of NF-kappaB inducing kinase ( Ad5NIK ) or IKKbeta ( Ad5IKKbeta ) strongly activated IKK activity ( > 20 fold ) in HT-29 cells with concomitant endogenous IkappaBalpha serine 32 phosphorylation and total IkappaBalpha degradation
Kim et al., Toxicol Appl Pharmacol 2004 (Drug-Induced Liver Injury) : Interestingly, it abrogated cisplatin induced DNA binding activity of nuclear factor-kappaB (NF-kappaB), which consequently affected mRNA expression levels of NF-kappaB dependent genes, inducible nitric oxide synthase (iNOS) , and cyclooxygenase-2 (COX-2), even in part ... Interestingly, it abrogated cisplatin induced DNA binding activity of nuclear factor-kappaB (NF-kappaB), which consequently affected mRNA expression levels of NF-kappaB dependent genes, inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) , even in part
Jang et al., J Cell Physiol 2004 (Leukemia, Erythroblastic, Acute) : We show that : ( 1 ) downregulation of Notch-1 sensitizes MEL cells to apoptosis induced by a Ca ( 2+ ) influx or anti-neoplastic drugs ; ( 2 ) Notch-1 downregulation induces phosphorylation of c-Jun N-terminal kinase (JNK) while constitutive activation of Notch-1 or prolonged exposure to a soluble Notch ligand abolishes it ; ( 3 ) Notch-1 has dose- and time dependent effects on the levels of apoptotic inhibitor Bcl-x ( L ) and cell cycle regulators p21 ( cip1/waf1 ), p27 ( kip1 ), and Rb ; and ( 4 ) Notch-1 activation by a cell associated ligand is accompanied by rapid and transient induction of NF-kappaB DNA binding activity
Lgssiar et al., Experimental biology and medicine (Maywood, N.J.) 2004 (Diabetes Mellitus, Experimental) : Interleukin-11 inhibits NF-kappaB and AP-1 activation in islets and prevents diabetes induced with streptozotocin in mice ... Furthermore, the effect of recombinant human ( rh ) IL-11 on MLD-STZ diabetes, insulitis, cytokines, IKK-alpha, NF-kappaB , and AP-1 was analyzed in islets ... Interleukin-11 prevented diabetes without affecting insulitis ; attenuated TNF-alpha and IFN-gamma response ; and stimulated IL-4 production and inhibited activation of IKK-alpha, NF-kappaB , and AP-1
Nakai et al., Br J Dermatol 2004 : However, long-term exposure ( 10 days ) to high glucose downregulates NF-kappaB binding activity and inhibits iNOS transcription and its activity ... Pretreatment with high glucose for 10 days down-regulated NF-kappaB activity and inhibited iNOS transcription and NO production, implying the involvement of a deficiency in NO synthesis in both skin infection and impaired wound healing in diabetic patients
Song et al., Neurosci Res 2004 : However, overexpression of Bcl-2 reduced Abeta induced apoptosis, and suppressed the activation of p38 MAP kinase and NF-kappaB ... Moreover, inhibition of NF-kappaB activation by sodium salicylates reduced Abeta induced apoptosis and activation of p38 MAP kinase, and up regulated Bcl-2 expression
Chandramohan et al., J Immunol 2004 : B cell receptor (BCR) engagement of murine WEHI 231 immature B lymphoma cells leads sequentially to a drop in NF-kappa B and c-Myc, and induction of the p27 ( Kip1 ) cyclin dependent kinase inhibitor, which promotes growth arrest and apoptosis ... B cell receptor (BCR) engagement of murine WEHI 231 immature B lymphoma cells leads sequentially to a drop in NF-kappa B and c-Myc, and induction of the p27 ( Kip1 ) cyclin dependent kinase inhibitor, which promotes growth arrest and apoptosis
Strassheim et al., J Immunol 2004 (Inflammation) : PI3-K blockade did not inhibit nuclear translocation of NF-kappa B , but did prevent Ser ( 536 ) phosphorylation of the p65 subunit of NF-kappa B, an event required for maximal transcriptional activity of NF-kappa B
Jaramillo et al., J Immunol 2004 (MAP Kinase Signaling System) : MSU crystals exerted their up-regulating effect by increasing extracellular signal regulated kinase ( ERK ) 1/2 phosphorylation and NF-kappa B nuclear translocation in response to IFN-gamma
Kato et al., J Am Soc Nephrol 2004 (Peritonitis) : mRNA expression of MCP-1 was abolished by NF-kappaB inhibition, but were not affected by the inhibition of ERK, JNK, or p38 ... mRNA expression of MCP-1 was abolished by NF-kappaB inhibition, but were not affected by the inhibition of ERK , JNK, or p38 ... As compared with MCP-1, MIP-2 mRNA expression was inhibited by a high dose of curcumin but not by NF-kappaB decoy oligodeoxynucleotide and individual inhibitions of MAP kinase, suggesting that the additional signaling pathway with NF-kappaB might be involved in mRNA expression of MIP-2 ... These show that TLR4 is directly involved in the production of MCP-1 and MIP-2 by MPMC in a NF-kappaB dependent manner, but the process does not require any MAP kinase activation ... These show that TLR4 is directly involved in the production of MCP-1 and MIP-2 by MPMC in a NF-kappaB dependent manner, but the process does not require any MAP kinase activation
Fujioka et al., J Biol Chem 2004 : Consequently, NF-kappaB dependent p53 activity induced the expression of p53 regulated genes PUMA and p21 ( waf1 ) as well as apoptosis ... Importantly, lack of RelA, IKK, and p53 as well as expression of a dominant negative IkappaBalpha ( IkappaBalphaM ) inhibited NF-kappaB dependent p53 activation and apoptosis
Kim et al., Toxicol Lett 2004 : However, pretreating the cells with o, p'-DDT and the NF-kappaB pathway inhibitors suppressed DDT induced NF-kappaB activation
Chae et al., Immunopharmacol Immunotoxicol 2004 : Salicylate regulates COX-2 expression through ERK and subsequent NF-kappaB activation in osteoblasts ... TNF-alpha plus IFN-gamma can induce extracellular signal regulated kinase ( ERK ), IKK, IkappaB degradation and NF-kappaB activation ... TNF-alpha plus IFN-gamma can induce extracellular signal regulated kinase ( ERK ), IKK, IkappaB degradation and NF-kappaB activation ... Salicylate treatment inhibited COX-2 expression induced by TNF-alpha/IFN-gamma and regulated the activation of ERK, IKK and IkappaB degradation and subsequent NF-kappaB activation in MC3T3E1 osteoblasts ... Salicylate treatment inhibited COX-2 expression induced by TNF-alpha/IFN-gamma and regulated the activation of ERK , IKK and IkappaB degradation and subsequent NF-kappaB activation in MC3T3E1 osteoblasts
Damiano et al., Biochem J 2004 : NF-kappaB ( nuclear factor kappaB ) reporter assays demonstrated that co-expression of either full-length CLAN or the NACHT domain of CLAN significantly inhibited NF-kappaB activation induced by Nod1 or Nod2 overexpression ... NF-kappaB ( nuclear factor kappaB ) reporter assays demonstrated that co-expression of either full-length CLAN or the NACHT domain of CLAN significantly inhibited NF-kappaB activation induced by Nod1 or Nod2 overexpression
Zhong et al., Anesthesiology 2004 (Myocardial Infarction...) : APC with sevoflurane attenuated NF-kappaB activation and reduced the expression of tumor necrosis factor alpha, interleukin 1, intercellular adhesion molecule 1, and inducible nitric oxide synthase
Wajant et al., Vitam Horm 2004 : Second, TRAIL and some of its receptors are inducible by NFkappaB , disclosing the possibility of autoamplifying TRAIL signaling loops
Higuchi et al., Biochem Biophys Res Commun 2004 : Protein disulfide isomerase suppresses the transcriptional activity of NF-kappaB ... We report here that the transcriptional activity of NF-kappaB is negatively regulated by protein disulfide isomerase (PDI)
Wang et al., Biochim Biophys Acta 2004 (Sarcoma, Kaposi) : Furthermore, using DNA protein binding experiments, we have shown that IL-8 increased AP-1 and NF Kappa B activity in these cells
Kim et al., Cell Mol Life Sci 2004 (Astrocytoma...) : Proteasome inhibitors suppressed TRAIL mediated activation of NF-kappaB ; however, inhibition of the NF-kappaB pathway alone was not sufficient to enhance TRAIL mediated cell death
Bhattacharyya et al., Blood 2004 : Notably, IL-10 mediated inhibition of NF-kappaB coincided with suppressed IkappaB kinase (IKK) activity in vitro ... Furthermore, IL-10 blocked inducible Akt phosphorylation, and inhibitors of phosphatidylinositol 3-kinase (PI3K) effectively suppressed the activation of Akt, IKK, and NF-kappaB ... Furthermore, IL-10 blocked inducible Akt phosphorylation, and inhibitors of phosphatidylinositol 3-kinase (PI3K) effectively suppressed the activation of Akt, IKK, and NF-kappaB
Wheeler et al., J Nutr 2004 : Because the gene expression of interleukin-8 (IL-8) , the major human neutrophil chemoattractant, is dependent on activation of NF-kappa B , IL-8 gene expression in human lung epithelial ( A549 ) cells treated with human IL-1 beta was used as a model of IL-1 beta signal transduction
Tang et al., Plast Reconstr Surg 2004 : Expression levels of NIK, IKKalpha, IKKbeta, and NF-kappaB genes were significantly increased by bFGF at concentrations of 2 and 10 ng/ml. Western blot confirmed the increase of NF-kappaB in the tenocytes
Fedele et al., Eur J Immunol 2004 : De novo synthesized CD38 is enzymatically active, and its expression in mature ( m ) MDDC is dependent on NF-kappa B activity
Tao et al., Cell Signal 2004 : The Epstein-Barr virus ( EBV ) latent membrane protein 1 (LMP1) oncoprotein may cause multiple cellular changes including the induction of epidermal growth factor receptor (EGFR) expression and activation of the NFkappaB transcription factor ... Inhibition of LMP1 mediated NFkappaB activation by constitutive repressive IkappaBalpha marginally decreased EGFR promoter activity using transiently transfected IkappaBalpha dominant negative mutant ... Taken together, Epstein-Barr virus latent membrane protein 1 modulated the EGFR promoter activity in a NFkappaB dependent manner
Kilpatrick et al., Am J Physiol Cell Physiol 2004 : Both kinases associate with TNFR-1 in response to TNF and are required for TNFR-1 serine phosphorylation, NF-kappaB activation, and inhibition of apoptosis
Satoh et al., Am J Physiol Gastrointest Liver Physiol 2004 : PKC-delta and -epsilon regulate NF-kappaB activation induced by cholecystokinin and TNF-alpha in pancreatic acinar cells ... PKC-delta and -epsilon regulate NF-kappaB activation induced by cholecystokinin and TNF-alpha in pancreatic acinar cells ... Inhibition of the novel PKC isoforms but not the conventional or the atypical isoform resulted in the prevention of NF-kappaB activation induced by CCK-8 and TNF-alpha ... Inhibition of the novel PKC isoforms but not the conventional or the atypical isoform resulted in the prevention of NF-kappaB activation induced by CCK-8 and TNF-alpha ... NF-kappaB activation by CCK-8 and TNF-alpha required translocation but not tyrosine phosphorylation of PKC-delta ... NF-kappaB activation by CCK-8 and TNF-alpha required translocation but not tyrosine phosphorylation of PKC-delta ... Activation of PKC-delta, PKC-epsilon, and NF-kappaB with CCK-8 involved both phosphatidylinositol-specific PLC and phosphatidylcholine ( PC ) -specific PLC, whereas with TNF-alpha they only required PC-specific PLC for activation
Suhara et al., Hypertension 2004 (Arteriosclerosis) : Hcy increased NF-kappaB DNA binding activity, and adenovirus mediated transfection of a Ikappa-B mutant ( Ikappa-B mt ) gene inhibited Hcy induced Fas expression
Wickremasinghe et al., J Biol Chem 2004 (Tuberculosis, Pulmonary) : Monocyte alveolar, but not upper airway, epithelial cell networks in pulmonary tuberculosis cause AP-1-, NF-IL-6-, and NFkappaB dependent CCL5 secretion
Lu et al., Proc Natl Acad Sci U S A 2004 (Neoplasms...) : Secreted transforming growth factor beta2 activates NF-kappaB , blocks apoptosis, and is essential for the survival of some tumor cells
Slogoff et al., J Gastrointest Surg 2004 (Acute Disease...) : COX-2 inhibition resulted in decreased inflammation and a decrease in NF-kappaB activation
Gil et al., Mol Cell Biol 2004 : The double stranded RNA ( dsRNA ) -dependent protein kinase PKR activates NF-kappa B via the I kappa B kinase (IKK) complex, but little is known about additional molecules that may be involved in this pathway
Saleem et al., Oncogene 2004 (Edema...) : We found that Lupeol treatment to mouse skin resulted in the inhibition of TPA induced ( i ) activation of PI3K, ( ii ) phosphorylation of Akt at Thr ( 308 ), ( iii ) activation of NF-kappaB and IKKalpha, and ( iv ) degradation and phosphorylation of IkappaBalpha ... We found that Lupeol treatment to mouse skin resulted in the inhibition of TPA induced ( i ) activation of PI3K, ( ii ) phosphorylation of Akt at Thr ( 308 ), ( iii ) activation of NF-kappaB and IKKalpha, and ( iv ) degradation and phosphorylation of IkappaBalpha
Hentze et al., Hepatology 2004 : CPT treatment completely abrogated the TNF induced NF-kappa B activation, and mRNA expression of the antiapoptotic factors TNF-receptor associated factor 2 , FLICE-inhibitory protein, and X-linked inhibitor of apoptosis protein was also inhibited by CPT. The caspase inhibitors benzyloxycarbonyl-Val-Ala-Asp- ( OMe ) -fluoromethylketone ( zVAD-fmk ) and benzyloxycarbonyl-Asp ( OMe ) -Glu ( OMe ) -Val-Asp ( OMe ) -chloromethylketone ( zDEVD-fmk ), as well as depletion of intracellular ATP by fructose prevented CPT/TNF induced apoptosis ... CPT treatment completely abrogated the TNF induced NF-kappa B activation, and mRNA expression of the antiapoptotic factors TNF-receptor associated factor 2, FLICE-inhibitory protein, and X-linked inhibitor of apoptosis protein was also inhibited by CPT. The caspase inhibitors benzyloxycarbonyl-Val-Ala-Asp- ( OMe ) -fluoromethylketone ( zVAD-fmk ) and benzyloxycarbonyl-Asp ( OMe ) -Glu ( OMe ) -Val-Asp ( OMe ) -chloromethylketone ( zDEVD-fmk ), as well as depletion of intracellular ATP by fructose prevented CPT/TNF induced apoptosis ... CPT treatment completely abrogated the TNF induced NF-kappa B activation, and mRNA expression of the antiapoptotic factors TNF-receptor associated factor 2, FLICE-inhibitory protein, and X-linked inhibitor of apoptosis protein was also inhibited by CPT. The caspase inhibitors benzyloxycarbonyl-Val-Ala-Asp- ( OMe ) -fluoromethylketone ( zVAD-fmk ) and benzyloxycarbonyl-Asp ( OMe ) -Glu ( OMe ) -Val-Asp ( OMe ) -chloromethylketone ( zDEVD-fmk ), as well as depletion of intracellular ATP by fructose prevented CPT/TNF induced apoptosis ... CPT treatment completely abrogated the TNF induced NF-kappa B activation, and mRNA expression of the antiapoptotic factors TNF-receptor associated factor 2, FLICE-inhibitory protein, and X-linked inhibitor of apoptosis protein was also inhibited by CPT. The caspase inhibitors benzyloxycarbonyl-Val-Ala-Asp- ( OMe ) -fluoromethylketone ( zVAD-fmk ) and benzyloxycarbonyl-Asp ( OMe ) -Glu ( OMe ) -Val-Asp ( OMe ) -chloromethylketone ( zDEVD-fmk ), as well as depletion of intracellular ATP by fructose prevented CPT/TNF induced apoptosis
Yao et al., J Hepatol 2004 : We also observed activation of ERK1/2, p38 MAPK, Akt and NF-kappaB signaling pathways by HGF in WB-F344 cells
Lappas et al., J Clin Endocrinol Metab 2004 : We previously demonstrated that, in human intrauterine tissues, lipopolysaccharide (LPS) stimulated nuclear factor-kappaB (NF-kappaB) DNA binding activity, and subsequent cytokine release can be suppressed by sulfasalazine ( SASP ) concentrations greater than 5 mM
Ren et al., Laryngoscope 2004 (Carcinoma...) : LMP1 induces IL-8 mainly through the activation of NF-kappa B and partly through AP-1 in NPC model cell lines, NPC-KT, and this suggests that LMP1 plays an important role in the angiogenesis of NPC ... LMP1 induces IL-8 mainly through the activation of NF-kappa B and partly through AP-1 in NPC model cell lines, NPC-KT, and this suggests that LMP1 plays an important role in the angiogenesis of NPC
Bharti et al., J Immunol 2004 : Treatment of these cells with RANKL activated NF-kappaB, and preexposure of the cells to curcumin completely suppressed RANKL induced NF-kappaB activation ... Thus overall these results indicate that RANKL induces osteoclastogenesis through the activation of NF-kappaB , and treatment with curcumin inhibits both the NF-kappaB activation and osteoclastogenesis induced by RANKL
Rothfuchs et al., J Immunol 2004 : Such NF-kappaB activation was independent of IFN-alphabeta, STAT1 , and RNA dependent protein kinase ... Such NF-kappaB activation was independent of IFN-alphabeta, STAT1 , and RNA dependent protein kinase
Yang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2003 : CSE could induce NF-kappaB activation and decrease level of I-kappaB ... L-Arg augmented CSE induced NF-kappaB activation and decreased I-kappaB content at low concentration while inhibited CSE induced NF-kappaB activation at high concentration, but there was not any significant difference of the expression of I-kappaB ... L-Arg augmented CSE induced NF-kappaB activation and decreased I-kappaB content at low concentration while inhibited CSE induced NF-kappaB activation at high concentration, but there was not any significant difference of the expression of I-kappaB ... NO may up-regulate and down-regulate the activation of NF-kappaB induced by CSE according to difference in NO concentration in AMs
Lee et al., Biol Pharm Bull 2004 : In cells transiently transfected with nuclear factor (NF)-kappaB promoter-luciferase reporter construct, this compound clearly inhibited the LPS stimulated NF-kappaB activation
Guo et al., Curr Opin Immunol 2004 : PKCbeta is indispensable for B-cell antigen receptor (BCR) induced NF-kappaB activation and B-cell survival ... Recent evidence indicates that PKCbeta might regulate inhibitor of kappaB kinase (IKK) and NF-kappaB activation through interaction with the CARMA1/Bcl10/MALT signaling complex in BCR microdomains
Cuschieri et al., Cell Immunol 2004 : Subsequently, LPS induced the degradation of IkappaB, and the nuclear activation of NF-kappaB and AP-1
Okamoto et al., Clin Diagn Lab Immunol 2004 (Neoplasms, Experimental) : TLR4 mediated NF-kappaB activation induced by AILb-A but not by lipopolysaccharide (LPS) was also observed under serum-free conditions ... TLR4 mediated NF-kappaB activation induced by AILb-A but not by lipopolysaccharide (LPS) was also observed under serum-free conditions
Weldon et al., Int J Oncol 2004 (Breast Neoplasms) : The effect of p38 inhibition on NF-kappaB transcriptional activation was analyzed
Lee et al., Int J Mol Med 2004 (Pneumonia) : An aqueous extract of Platycodi radix inhibits LPS induced NF-kappaB nuclear translocation in human cultured airway epithelial cells ... LPS induced expression of I-kappaBalpha, which is expressed by LPS induced activation of NF-kappaB , is inhibited by AEPR as well
Jeong et al., Pharm Res 2004 : ITCs such as phenethyl isothiocyanate ( PEITC ), sulforaphane ( SUL ), allyl isothiocyanate ( AITC ), and curcumin ( CUR ) strongly inhibited LPS induced NF-kappaB-luciferase activations, whereas RES increased activation at lower dose, but inhibited activation at higher dose, and tea flavonoids and procyanidin dimers had little or no effects
Li et al., Mol Immunol 2004 : We demonstrated that overexpression of Tollip inhibited NFkB reporter gene transcription ... On the contrary, overexpression of Tollip ( KE ) mutant has no inhibitory effect on LPS induced NFkB reporter activity
Jang et al., J Biol Chem 2004 : Downregulation of PIAS3 by RNA interference reverses its effect on TNF-alpha mediated NF-kappaB activation
Tarabin et al., Naunyn Schmiedebergs Arch Pharmacol 2004 : Expression of IkappaBalpha-SR inhibited NF-kappaB stimulated by tumor necrosis factor alpha (TNFalpha) and 6-OHDA
van Holten et al., Arthritis Res Ther 2004 (Arthritis, Experimental...) : We also examined the effect of IFN-beta on NF-kappaB activity
Yeh et al., J Biol Chem 2004 (Inflammation) : We have shown recently that Ox-PAPC mediated induction of IL-8 transcription is independent of NF-kappaB activation, a major transcription factor utilized by cytokines and lipopolysaccharide for the induction of IL-8 transcription
Wurzer et al., J Biol Chem 2004 : NF-kappaB dependent induction of tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) and Fas/FasL is crucial for efficient influenza virus propagation ... NF-kappaB dependent induction of tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) and Fas/FasL is crucial for efficient influenza virus propagation ... On a molecular level this is due to NF-kappaB dependent viral induction of the proapoptotic factors tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) and FasL , which enhance virus propagation in an autocrine and paracrine fashion
Meyer-Bahlburg et al., Clin Exp Rheumatol 2004 : TNF-alpha expression can be mediated by transcription factor nuclear factor-kappa B (NF-kappaB) ... In vitro expression of TNF-alpha and activation of NF-kappaB in synovial fibroblasts after infection with Yersinia enterocolitica or Salmonella enteritidis was analysed by electrophoretic mobility shift assay, Western blot assay and real-time PCR
Hwang et al., Biochem Biophys Res Commun 2004 : The inhibitory effect of LY294002 on NO generation may be mediated through specific inhibition of signal transducer and activator-1 ( STAT1 ) and NF-kappaB , which are activated by IFN-gamma
Kim et al., J Neuroimmunol 2004 : Sodium butyrate significantly inhibited NF-kappa B binding and NF-kappa B-mediated transcription induced by IFN-gamma , suggesting that the anti-inflammatory effect of sodium butyrate is mediated via specific inhibition of the NF-kappa B pathway
Onodera et al., Arthritis Rheum 2004 (Arthritis, Rheumatoid...) : The mRNA up-regulation of IL-8 and IL-1beta by MIF was inhibited by 2 tyrosine kinase inhibitors, a protein kinase C ( PKC ) inhibitor, an activator protein 1 (AP-1) inhibitor, and by an NF-kappaB inhibitor ... The mRNA up-regulation of IL-8 and IL-1beta by MIF was inhibited by 2 tyrosine kinase inhibitors, a protein kinase C ( PKC ) inhibitor, an activator protein 1 (AP-1) inhibitor, and by an NF-kappaB inhibitor ... The mRNA up-regulation of IL-8 and IL-1beta by MIF was inhibited by 2 tyrosine kinase inhibitors, a protein kinase C ( PKC ) inhibitor, an activator protein 1 (AP-1) inhibitor , and by an NF-kappaB inhibitor ... MIF enhanced AP-1 and NF-kappaB binding activities in a dose dependent manner
Iwahashi et al., Arthritis Rheum 2004 (Arthritis, Rheumatoid) : NF-kappaB activation in adhered monocytes was induced by LTA , but this NF-kappaB activity was not augmented by anti-FcgammaRIII antibody stimulation ... NF-kappaB activation in adhered monocytes was induced by LTA, but this NF-kappaB activity was not augmented by anti-FcgammaRIII antibody stimulation
Shibuya et al., Life Sci 2004 : BAY11-7082, an inhibitor of nuclear factor kappaB (NF-kappaB) activation, and MG132, a 26S proteasome inhibitor, reduced the TPA induced ICAM-1 expression but not the IFN-gamma induced one
De Ketelaere et al., FEBS Lett 2004 : Involvement of GSK-3beta in TWEAK mediated NF-kappaB activation ... Involvement of GSK-3beta in TWEAK mediated NF-kappaB activation
Je et al., FEBS Lett 2004 (MAP Kinase Signaling System) : HHE increased the activity of p38 MAPK and extracellular signal regulated kinase ( ERK ), but not c-jun NH ( 2 ) -terminal kinase, indicating that p38 MAPK and ERK are closely involved in HHE induced NF-kappaB transactivation ... HHE increased the activity of p38 MAPK and extracellular signal regulated kinase ( ERK ), but not c-jun NH ( 2 ) -terminal kinase, indicating that p38 MAPK and ERK are closely involved in HHE induced NF-kappaB transactivation ... These findings strongly suggest that HHE induces NF-kappaB activation through IKK/NIK pathway and/or p38 MAPK and ERK activation associated with oxidative stress in endothelial cells ... These findings strongly suggest that HHE induces NF-kappaB activation through IKK/NIK pathway and/or p38 MAPK and ERK activation associated with oxidative stress in endothelial cells
Palomba et al., J Biol Chem 2004 : Because NO suppresses the NFkappaB dependent NOS-II expression, inactivation of NOS-I by the LPS/IFNgamma induced AA pathway provides optimal conditions for NFkappaB activation and subsequent NOS-II expression ... Inhibition of cPLA2 activity, while reducing the availability of AA, consistently inhibited NFkappaB activation and NOS-II mRNA induction and delayed NO formation ... Inhibition of cPLA2 activity, while reducing the availability of AA, consistently inhibited NFkappaB activation and NOS-II mRNA induction and delayed NO formation
Yeung et al., EMBO J 2004 : In this study, we demonstrate that SIRT1 , a nicotinamide adenosine dinucleotide dependent histone deacetylase, regulates the transcriptional activity of NF-kappaB
Tsujimura et al., J Immunol 2004 : Toll-like receptor 9 signaling activates NF-kappaB through IFN regulatory factor-8/IFN consensus sequence binding protein in dendritic cells
Kim et al., J Immunol 2004 : In contrast, TGF-beta1 and LY294002 enhanced LPS induced NF-kappaB activity ... In contrast, TGF-beta1 and LY294002 enhanced LPS induced NF-kappaB activity
Powell et al., Clin Dev Immunol 2004 : We determined that the marked synergy between LPS and IFN-gamma on MIG mRNA expression in mouse macrophages is a result of LPS induced NF-kappaB and IFN-gamma induced STAT
Casal et al., Neurosci Res 2004 (Translocation, Genetic) : The Abeta 1/42 peptide induced the activation of NF-kappaB in all studied cell cultures and its effect was potentiated by interferon-gamma (IFN-gamma) ... Abeta 25/35 alone did not induce NF-kappaB activation, but Abeta 25/35 plus IFN-gamma induced the activation of the transcription factor in the mixed and pure microglial cultures, although not in pure astroglia ... Abeta 25/35 alone did not induce NF-kappaB activation, but Abeta 25/35 plus IFN-gamma induced the activation of the transcription factor in the mixed and pure microglial cultures, although not in pure astroglia
Yang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2003 : [ Effects of polymyxin B on LPS induced activation of NF-kappaB in pulmonary alveolar macrophages ] ... To observe effects of polymyxin B ( PMB ) on LPS induced activation of NF-kappaB of pulmonary alveolar macrophages ( PAMs ) and explore the possible anti-inflammation efficiency of PMB ... LPS can induce IKK-beta and NF-kappaB activation, IkappaB-alpha degradation, accelerate TNF-alpha release, but PMB can counteract those effects induced by LPS stimulation
Marchetti et al., J Biol Chem 2004 : Both TNFR1 and TNFR2 induced the NF-kappa B pathway, yet with distinguishable kinetics and upstream activating components, TNFR1 only induced transient NF-kappa B activation, whereas TNFR2 facilitated long term phosphatidylinositol 3-kinase dependent NF-kappa B activation strictly ... Both TNFR1 and TNFR2 induced the NF-kappa B pathway, yet with distinguishable kinetics and upstream activating components, TNFR1 only induced transient NF-kappa B activation, whereas TNFR2 facilitated long term phosphatidylinositol 3-kinase dependent NF-kappa B activation strictly ... Glutamate induced triggering of the ionotropic N-methyl-D-aspartate receptor was required for the enhanced and persistent phosphatidylinositol 3-kinase dependent NF-kappa B activation by TNFR2 , indicating a positive cooperation of TNF and neurotransmitter induced signal pathways ... TNFR2 induced persistent NF-kappa B activity was essential for neuronal survival
Chu et al., Mol Endocrinol 2004 (Granulosa Cell Tumor...) : Repression of ERbeta signaling by NF-kappaB has not previously been reported
Jimi et al., Nat Med 2004 (Arthritis, Experimental...) : The peptide inhibited RANKL stimulated NF-kappa B activation and osteoclastogenesis both in vitro and in vivo
Kitatani et al., Cell Signal 2004 : SMase also augmented TNF-alpha mediated nuclear factor kappaB (NF-kappaB) activation
Mori et al., Life Sci 2004 : In ECV304 cells overexpressing ERalpha, E ( 2 ) significantly inhibited the effects of TNF-alpha on NF-kappaB activation, VCAM-1 expression, and U937 cell adhesion ... The mechanism of suppression may involve inhibition of NF-kappaB mediated up-regulation of VCAM-1 expression induced by atherogenic stimuli
Guo et al., Clin Cancer Res 2004 : Reduction of endogenous p65 by siRNA treatment significantly impaired CPT-11 mediated NF-kappaB activation, enhanced apoptosis, and reduced colony formation in soft agar
Sarkar et al., J Biol Chem 2004 (Breast Neoplasms...) : Our results show that mangiferin blocks tumor necrosis factor (TNF) induced NF-kappaB activation and NF-kappaB dependent genes like ICAM1 and COX2 ... In addition, mangiferin inhibits TNF induced p65 phosphorylation as well as translocation to the nucleus and also inhibits NF-kappaB activation induced by other inflammatory agents like PMA, ceramide, and SA-LPS
Ye et al., J Pharmacol Exp Ther 2004 (Colonic Neoplasms) : However, 5-lipoxygenase inhibition had no effect on alpha7-nAChR mRNA expression, but significantly inhibited cell proliferation and activation of NF-kappaB and cyclooxygenase-2, whereas NF-kappaB-specific inhibitor caffeic acid phenethyl ester reduced both cell proliferation and cyclooxygenase expression induced by NNK without affecting alpha7-nAChR mRNA level and 5-lipoxygenase expression
Lee et al., Proc Natl Acad Sci U S A 2004 (Fever...) : Whereas IL-1beta induced the expression of the NF-kappaB-reporter gene and was suppressed by competitive inhibition of NF-kappaB binding, IL-18 responses were weak or absent
Cowburn et al., Eur J Immunol 2004 : In human neutrophils, TNF-alpha ( 200 U/ml ) induced rapid I kappa B-alpha degradation, NF-kappa B activation and IL-8 release ( 31.8+/-5.4 pg/10 ( 5 ) cells/2 h ), whereas GM-CSF ( 10 ng/ml ) stimulated an equivalent IL-8 release ( 26.5+/-4.5 pg/10 ( 5 ) cells/2 h ) without enhanced I kappa B-alpha degradation or NF-kappa B activation compared to control ... These data suggest that cross-talk between NF-kappa B and PI3-kinase pathways in TNF-alpha -stimulated neutrophils results from NF-kappa B/ERK1/2 dependent IL-8 production which acts in an autocrine manner to drive PI3-kinase dependent survival
Hachiya et al., Cytokine 2004 : These results indicate that endogenous production of TNFalpha in macrophages/monocytes is required for NFkappaB activation by irradiation
Yang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2003 : NAP2 could activate NF-kappaB and IL-12 promoter
Jiménez Del Río et al., Arch Med Res 2004 (Necrosis...) : Further evaluations comprised production of H2O2, generation of hydroxyl radical ( .OH ), disruption of mitochondrial transmembrane potential ( DeltaPsim ), caspase-3 activation, and activation of NF-kappaB and p53 transcriptional factors ... All metals induced mitochondrial depolarization ( 17-62 % nonfluorescent cells ) and activated caspase-3 concomitantly with apoptotic morphology ( 25-32 % AO/EB ) at 24 h, and neither NF-kappaB nor p53 transcription factor showed activation
Giguère et al., J Virol 2004 : Moreover, we demonstrate that NF-kappaB is induced by CD80- and CD86 bearing virions when they are combined with the engagement of the T-cell receptor/CD3 complex, an event that is inhibited upon surface expression of CTLA-4
Rödel et al., Int J Radiat Biol 2004 : The induction by NF-kappaB of TGF-beta1 in endothelial cells might contribute to the anti-inflammatory properties of low-dose ionizing irradiation
Zhang et al., J Huazhong Univ Sci Technolog Med Sci 2004 : In order to investigate the effect of nuclear factor-kappa B (NF-kappaB) on vascular endothelial growth factor ( VEGF ) mRNA expression of human pulmonary artery smooth muscle cells ( HPASMCs ) in hypoxia, the cultured HPASMCs in vitro were stimulated with pyrrolidine dithiocarbamate ( PDTC ), an inhibitor of NF-kappaB ... It is suggested that the activation of NF-kappaB is involved in the VEGF mRNA expression of HPASMCs under hypoxia
Ono et al., Shock 2004 (Hepatitis, Alcoholic...) : We previously demonstrated that production of interleukin-6 (IL-6) and granulocyte colony stimulating factor ( G-CSF ), mediated by nuclear factor-kappa B (NF-kappa B) , each make essential contributions to organ injury and inflammation in a rodent model of controlled HS, and we proposed in this study to examine the hypothesis that the increased susceptibility to MOF after shock/trauma in the setting of chronic ethanol use is due to an exaggerated activation of NF-kappa B and production of these proinflammatory cytokines ... We previously demonstrated that production of interleukin-6 (IL-6) and granulocyte colony stimulating factor ( G-CSF ), mediated by nuclear factor-kappa B (NF-kappa B) , each make essential contributions to organ injury and inflammation in a rodent model of controlled HS, and we proposed in this study to examine the hypothesis that the increased susceptibility to MOF after shock/trauma in the setting of chronic ethanol use is due to an exaggerated activation of NF-kappa B and production of these proinflammatory cytokines
Grempler et al., Biochem J 2004 (Carcinoma, Hepatocellular...) : TNFalpha , but not insulin, led to rapid activation of NFkappaB ( nuclear factor kappaB ) ... TNFalpha, but not insulin , led to rapid activation of NFkappaB ( nuclear factor kappaB ) ... In conclusion, the data indicate that the activation of NFkappaB is sufficient to suppress G6Pase gene expression, and is required for the regulation by TNFalpha, but not by insulin
Ichiyama et al., Inflamm Res 2004 : We examined whether or not IVIG inhibits TNF-alpha induced activation of transcription factor NF-kappaB , a factor that is essential for the expression of proinflammatory cytokines, in human coronary artery endothelial cells ( CAEC ) ... The inhibitory effect of IVIG on NF-kappaB activation induced by TNF-alpha was evaluated by Western blot analysis and ELISA ... Western blot analysis and ELISA demonstrated that IVIG inhibits NF-kappaB activation induced by TNF-alpha in CAEC ... The data suggest that IVIG inhibits NF-kappaB activation induced by TNF-alpha in CAEC, thereby possibly modulating IL-6 production and E-selectin expression
Agustí et al., Thorax 2004 (Body Weight...) : NF-kappaB activation and iNOS induction occur in skeletal muscle of COPD patients with low body weight
Zbytek et al., J Endocrinol 2004 (Translocation, Genetic) : CRH dependent stimulation of NF-kappaB activity is consistent with accumulated data about role of this neuropeptide in the regulation of local epidermal homeostasis
Sodin-Semrl et al., Int J Immunopathol Pharmacol 2004 (Arthritis, Rheumatoid) : Opposing regulation of interleukin-8 and NF-kappaB responses by lipoxin A4 and serum amyloid A via the common lipoxin A receptor ... SAA up-regulated NF-kappaB and AP-1 DNA binding activity, while LXA4 markedly inhibited these responses after IL-1beta stimulation ... A human IL-8 promoter luciferase construct was transfected into CHO cells stably expressing ALXR in order to determine the role of NF-kappaB and/or AP-1 in the regulation of IL-8 gene expression
Han et al., Life Sci 2004 : EMSA demonstrated that ABL inhibited effectively the association of NF-kappaB , which is necessary for the expression of iNOS and COX-2, with its binding motif in the promoter of target genes ... The anti-inflammatory effect of ABL involves blocking the binding of NF-kappaB to the promoter in the target genes and inhibiting the expression of iNOS and COX-2 ... The anti-inflammatory effect of ABL involves blocking the binding of NF-kappaB to the promoter in the target genes and inhibiting the expression of iNOS and COX-2
Witherow et al., Proc Natl Acad Sci U S A 2004 : Conversely, suppression of beta-arrestin 1, but not beta-arrestin 2, expression by using RNA interference led to a 3-fold increase in tumor necrosis factor stimulated NF-kappaB activity as measured by NF-kappaB mobility-shift analysis
Shi et al., J Cardiovasc Pharmacol 2004 : Electrophoresis mobility shift assay ( EMSA ) and Western blot analysis showed that both the nuclear factor-kappaB (NF-kappaB) and the specificity protein-1 (SP-1) were activated by TNFalpha ... However, NAC only partially inhibited the TNFalpha induced activation of NF-kappaB , but abolished the activation of SP-1 ... However, NAC only partially inhibited the TNFalpha induced activation of NF-kappaB , but abolished the activation of SP-1
Ogasawara et al., J Bone Miner Res 2004 : Osteoclast differentiation by RANKL requires NF-kappaB mediated downregulation of cyclin dependent kinase 6 (Cdk6)
Baus-Loncar et al., Peptides 2004 : TNF-alpha stimulation, which induces NF-kappaB activation, evoked up to 10-fold reduction of TFF3 expression in the colon tumour cell line HT-29
Tao et al., Virus Res 2004 : Nuclear factor kappa B (NFkappaB) dependent modulation of Epstein-Barr virus latent membrane protein 1 (LMP1) in epidermal growth factor receptor (EGFR) promoter activity ... The Epstein-Barr virus ( EBV ) latent membrane protein 1 (LMP1) oncoprotein may cause multiple cellular changes, including the induction of epidermal growth factor receptor (EGFR) expression and the activation of the nuclear factor kappa B (NFkappaB) transcription factor ... Inhibition of LMP1 mediated NFkappaB activation by constitutive repressive inhibitory kappa B alpha ( IkappaBalpha ) marginally decreased EGFR promoter activity using transiently transfected IkappaBalpha dominant negative mutant
Chen et al., Biochem Biophys Res Commun 2004 : GTPase RhoA is required for fMet-Leu-Phe ( fMLP ) -stimulated NF-kappaB activation in human peripheral blood monocytes ... These results indicate that fMLP stimulates three members of the Rho family of GTPases Rac1, Cdc42, and RhoA activity in monocytes, and that Rac1 and RhoA, but not Cdc42 , is required for fMLP induced NF-kappaB activation ... These results indicate that fMLP stimulates three members of the Rho family of GTPases Rac1, Cdc42, and RhoA activity in monocytes, and that Rac1 and RhoA , but not Cdc42, is required for fMLP induced NF-kappaB activation ... These results indicate that fMLP stimulates three members of the Rho family of GTPases Rac1, Cdc42, and RhoA activity in monocytes, and that Rac1 and RhoA, but not Cdc42, is required for fMLP induced NF-kappaB activation
García-Piñeres et al., Life Sci 2004 : In this contribution we analyzed the mechanism of the sesquiterpene lactone mediated inhibition using different cell systems, and showed that in all the cell lines analyzed, SLs inhibited both NF-kappaB binding and the IkappaB-kinase, but that the former played a more preponderant role in the inhibition
Stilo et al., J Biol Chem 2004 : However, the biochemical mechanism by which CARMA1 regulates activation of NFkappaB remains to be determined ... Expression of the NEMO binding region of CARMA3 exerts a dominant negative effect on Bcl10 mediated activation of NFkappaB
Reynaert et al., Proc Natl Acad Sci U S A 2004 : Because activation of inhibitory kappaB kinase (IKK), the prerequisite enzyme complex necessary to induce NF-kappaB, is subject to redox regulation, we assessed whether IKK could present a more proximal target for NO to inhibit NF-kappaB activation ... Collectively, our findings demonstrate that IKKbeta is an important target for the redox regulation of NF-kappaB by endogenous or exogenous NO, providing an additional mechanism for its antiinflammatory properties
Wang et al., Lab Invest 2004 (Brain Neoplasms...) : Functional correlation between Akt activation and the activation of NFkappaB was confirmed in U251MG GBM cells in which inhibition of Akt activation either by stable expression of PTEN or by the PI3-kinase inhibitors, wortmannin and LY294002, led to a concomitant decrease in NFkappaB binding activity ... Thus, our results demonstrate that constitutive activation of Akt and NFkappaB, but not Stat3, contributes significantly to the progression of diffuse gliomas, and activation of Akt may lead to NFkappaB activation in high-grade gliomas
Doan et al., J Immunol 2004 : Differential regulation of TNF-R1 signaling : lipid raft dependency of p42mapk/erk2 activation, but not NF-kappaB activation ... Differential regulation of TNF-R1 signaling : lipid raft dependency of p42mapk/erk2 activation, but not NF-kappaB activation
Zhang et al., Genes Chromosomes Cancer 2004 (Leukemia, Myeloid, Acute...) : PRDX4 plays a regulatory role in the activation of the transcription factor NF-kappaB and is significantly down-regulated in acute promyelocytic leukemia
Fouad et al., Liver Int 2004 (Necrosis) : We investigated if NF-kappaB was responsible for inducible nitric oxide synthase (iNOS) expression and cytoprotection induced by PGE1 against D-GalN cell death in cultured hepatocytes ... A kinetic study of cell death, NF-kappaB activation , mRNA and protein iNOS expression, and NO production was carried in hepatocytes treated with D-GalN ( 5 mM ) in the presence or absence of PGE1 ( 1 microM ) administered 2 h before the hepatotoxin ... The present study shows that the cytoprotection by PGE1 against D-GalN induced apoptosis was related to NF-kappaB dependent iNOS expression
Lee et al., Am J Respir Cell Mol Biol 2004 : Dexamethasone, a glucocorticoid, inhibited IL-1beta induced nuclear translocation of NF-kappaB and also the secretion of IL-13 from mast cells
Hu et al., Carcinogenesis 2004 (Cell Transformation, Neoplastic) : Insufficient p65 phosphorylation at S536 specifically contributes to the lack of NF-kappaB activation and transformation in resistant JB6 cells ... Deficient activation of p65 contributes to the lack of NF-kappaB activation in transformation-resistant ( P- ) cells ... We hypothesized that the differential NF-kappaB activation involves differential p65 phosphorylation arising from enzyme activity differences ... These findings provide direct evidence that phosphorylation of p65 at S536 is required for TNF-alpha induced NF-kappaB activation in the JB6 transformation model ... These findings provide direct evidence that phosphorylation of p65 at S536 is required for TNF-alpha induced NF-kappaB activation in the JB6 transformation model
Wang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2004 : Akt1 antisense ODN specifically decreased AIgG induced NF-kappa B activation, cyclin D1 mRNA and its protein expression, and then inhibited MCs to progress to S-phase and cell proliferation
Vallee et al., Exp Cell Res 2004 (Adenocarcinoma...) : Finally, it appeared that in polarized HT29-D4 cells, the IL-1beta induced translocation of NF-kappaB was connected to PKCdelta translocation
Hattori et al., Cardiovasc Res 2004 : Since immunostimulant induced iNOS gene expression is itself dependent on NF-kappaB , NO is expected to limit its own synthesis ... NO donors, but not ONOO-, suppressed LPS induced NF-kappaB activation and expression of a murine iNOS promoter/reporter construct
Mattson et al., Int J Hyperthermia 2004 : Heat shock and the activation of AP-1 and inhibition of NF-kappa B DNA binding activity : possible role of intracellular redox status ... Gel electromobility shift assays ( EMSA ) demonstrated that heat shock induced AP-1 DNA binding activity but inhibited IR-induced activation of NF-kappa B
Lamberti et al., Biol Chem 2004 : We found that human recombinant IL-7 induced NF-kappaB/Rel activation, analyzed by electrophoretic mobility shift assay ( EMSA ), in human peripheral blood T lymphocytes from healthy donors
Yu et al., J Mol Med (Berl) 2004 : The activity of NF-kappaB can be regulated by protein kinase A (PKA) in a cAMP independent manner
Olsen et al., Int J Cancer 2004 (Carcinoma, Non-Small-Cell Lung...) : CHS 828 is able to inhibit the lipopolysaccharide (LPS) induced nuclear localization as well as the transcriptional activity of NF-kappa B in human THP-1 leukemia cells
Ran et al., Genes Dev 2004 : We demonstrate here that Hsp70 promotes TNF killing by specifically binding the coiled-coil domain of I kappa B kinase gamma (IKK gamma) to inhibit IKK activity and consequently inhibit NF-kappa B-dependent antiapoptotic gene induction
Lu et al., Toxicological sciences : an official journal of the Society of Toxicology 2004 (Body Weight) : Hepatic NF-kappaB DNA binding activity and cell proliferation were increased by PCB-153 in wild-type mice but not in p50-/- mice
Fan et al., Shock 2004 (Inflammation...) : Pretreatment with PTx inhibited TLR4ca induced ERK 1/2 phosphorylation ( 30 +/- 7 %, P < 0.05 ) and AP-1 activation ( 36 +/- 3 %, P < 0.05 ) but did not inhibit NFkappaB activation ... Pretreatment with PTx inhibited TLR4ca induced ERK 1/2 phosphorylation ( 30 +/- 7 %, P < 0.05 ) and AP-1 activation ( 36 +/- 3 %, P < 0.05 ) but did not inhibit NFkappaB activation ... Pretreatment with PTx inhibited TLR4ca induced ERK 1/2 phosphorylation ( 30 +/- 7 %, P < 0.05 ) and AP-1 activation ( 36 +/- 3 %, P < 0.05 ) but did not inhibit NFkappaB activation
Foss et al., Leukemia & lymphoma 2003 (Lymphoma, T-Cell, Cutaneous) : Other novel agents include the interleukin (IL)-2 fusion toxin ( ONTAK ), pentostatin ( a potent adenosine deaminase inhibitor ), histone deacetylase inhibitors such as depsipeptide, NF-kappaB inhibitors , cytokine receptor antagonists, immunomodulatory therapies and allogeneic stem cell therapy
Schaecher et al., Neurochem Res 2004 : Human peripheral blood mononuclear cells ( PBMCs ) were activated using anti-CD3/CD28 ( HIT3A/CD28.2 ) resulting in degradation of IkB alpha , an inhibitor of NFkB , relative to unactivated cells
Rahman et al., Nutr Cancer 2004 (Breast Neoplasms) : In addition, I3C also abrogated the EGF induced activation of NF-kappaB , which was mediated via the Akt signaling pathway
Vitiello et al., Cytokine 2004 (MAP Kinase Signaling System) : Our results elucidate some of the molecular mechanisms by which AP-1 and NF-kappaB regulate IL-8 release and open new strategies for the design of specific molecules that will modulate IL-8 effects in various infectious diseases
Gingras et al., Biochem Biophys Res Commun 2004 : These results suggest that Neovastat induces tPA gene transcription through activation of the JNK and NFkappaB signaling pathways, leading to an increase of tPA secretion by endothelial cells
Hu et al., J Biol Chem 2004 : TNAP specifically inhibits NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha , TNF receptor 1, TRADD, RIP, TRAF2, and NIK but does not affect IKK1- and IKK2 mediated NF-kappaB activation ... TNAP specifically inhibits NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha, TNF receptor 1, TRADD , RIP, TRAF2, and NIK but does not affect IKK1- and IKK2 mediated NF-kappaB activation ... TNAP specifically inhibits NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha, TNF receptor 1, TRADD, RIP, TRAF2 , and NIK but does not affect IKK1- and IKK2 mediated NF-kappaB activation ... Knockdown of TNAP by lentiviral mediated small interference RNA potentiates TNF-alpha induced NF-kappaB activation
Jang et al., J Biol Chem 2004 : In addition, sustained activation of ERK1/2 ( upstream of NF-kappaB ) was observed in bcl-2 overexpressing cells
Seo et al., Oncogene 2004 : vIRF3 repressed NF-kappaB dependent transcription in a dose dependent manner and inhibited the activation of NF-kappaB induced by tumor necrosis factor (TNF)-alpha
Hwang et al., Oncogene 2004 (Stomach Neoplasms) : Electrophoretic mobility shift assay confirmed that IL-1beta increased the DNA binding activity of AP-1 and NF-kappaB ... The above results suggest that MAPK-AP-1 and ROS-NF-kappaB signaling pathways are involved in the IL-1beta induced IL-8 expression and that these paracrine signaling pathways induce endothelial cell proliferation
Oh et al., Immunopharmacol Immunotoxicol 2004 : Here, we demonstrate that dehydrocostus lactone ( DL ), the major sesquiterpene lactone isolated from the roots of Saussurea lappa, inhibits NF-kappaB activation by preventing TNF-alpha induced degradation and phosphorylation of its inhibitory protein I-kappaB alpha in human leukemia HL-60 cells and that DL renders HL-60 cells susceptible to TNF-alpha induced apoptosis by enhancing caspase-8 and caspase-3 activities
Chae et al., Immunopharmacol Immunotoxicol 2004 : TNF-gamma ( 10 ng/ml ) increased NF-kappaB DNA binding activity in nuclear extracts of osteoblasts ... The addition of NAC ( N-acetyl cysteine ), free radical scavenger, completely prevented TNF-alpha induced activation of NF-kappaB ... The addition of NAC ( N-acetyl cysteine ), free radical scavenger, completely prevented TNF-alpha induced activation of NF-kappaB ... Here, we suggest that the degradations of IkappaB alpha and IkappaB beta and the following activation of NF-kappaB are the targets of NAC and that NF-kappaB transcription factor is a pivotal clue to regulation of differentiation in TNFalpha exposed osteoblasts ... Here, we suggest that the degradations of IkappaB alpha and IkappaB beta and the following activation of NF-kappaB are the targets of NAC and that NF-kappaB transcription factor is a pivotal clue to regulation of differentiation in TNFalpha exposed osteoblasts
Lynch et al., Mol Interv 2004 : The molecular mechanisms by which NF-kappaB is activated by TCR-CD28 have only recently become known ... Overexpression of MALT1 , as observed in a subset of lymphoma patients, leads to the potent activation of NF-kappaB , suggesting that MALT1 might stimulate ( directly or indirectly ) the kinase complex [ IKK, inhibitor of NF-kappaB (IkappaB) kinase ] responsible for activating cytoplasmic NF-kappaB for translocation into the nucleus
Takahashi et al., Pancreas 2004 (Neoplasm Invasiveness...) : The invasion cell number and the NF-kappaB activity were increased by GDNF stimulation ... These results indicate that GDNF promotes NF-kappaB activation and that the latter is involved in the invasive potential of human pancreatic cancer cells
Jijon et al., Cell Signal 2004 : We report that although TNF induced NF-kappaB transcriptional activity is abolished in IKKgamma ( -/- ) cells, adenoviral gene delivery of NIK ( Ad5NIK ) still enhanced transcriptional activity and IL-6 mRNA accumulation ... We report that although TNF induced NF-kappaB transcriptional activity is abolished in IKKgamma ( -/- ) cells, adenoviral gene delivery of NIK ( Ad5NIK ) still enhanced transcriptional activity and IL-6 mRNA accumulation ... Functional analysis demonstrated that Ad5NIK induced NF-kappaB transcriptional activity, IL-6 mRNA expression and RelA phosphorylation are inhibited by the p38 inhibitor SB203580, suggesting a role for this MAPK in NIK signaling to NF-kappaB ... These data demonstrate for the first time the presence of an IKKgamma independent NIK/p38 MAPK dependent signaling pathway that activates NF-kappaB and induces pro-inflammatory gene expression through RelA phosphorylation
Nitta et al., Cell Signal 2004 (Nasopharyngeal Neoplasms) : Our recent report demonstrated that expression of LMP1 induced programmed cell death in an NF-kappaB dependent manner
Shan et al., J Biol Chem 2004 : Inhibition of NF-kappaB , and the activation of p38 MAPK were also found to be involved in 15d-PG J2-induced chondrocyte apoptosis
Liu et al., J Biol Chem 2004 : In a BCL10-deficient macrophage cell line, LPS induced NF-kappaB activation was consistently defective, whereas activator protein-1 and Elk-1 signaling was intact
Opitz et al., J Biol Chem 2004 : By dominant negative overexpression and small interfering RNA experiments, we show for the first time that interleukin-1 receptor associated kinase participates in Nod2 dependent NF-kappaB activation ... Additionally, dominant negative interleukin-1 receptor associated kinase 2, tumor necrosis factor receptor associated factor 6, NF-kappaB inducing kinase, transforming growth factor-beta activated kinase binding protein 2, and transforming growth factor-beta activated kinase 1 also inhibited Nod2 dependent NF-kappaB activation ... Additionally, dominant negative interleukin-1 receptor associated kinase 2, tumor necrosis factor receptor associated factor 6, NF-kappaB inducing kinase, transforming growth factor-beta activated kinase binding protein 2, and transforming growth factor-beta activated kinase 1 also inhibited Nod2 dependent NF-kappaB activation ... Furthermore, Rip-2 and members of the Toll-like receptor signaling cascade are involved in the Nod2 dependent activation of NF-kappaB induced by pneumococci
DiPerna et al., J Biol Chem 2004 : Poxvirus protein N1L targets the I-kappaB kinase complex , inhibits signaling to NF-kappaB by the tumor necrosis factor superfamily of receptors, and inhibits NF-kappaB and IRF3 signaling by toll-like receptors
Lee et al., Eur J Neurosci 2004 (Hyperalgesia...) : Spinal NF-kB activation induces COX-2 upregulation and contributes to inflammatory pain hypersensitivity ... Moreover, the transcription factor, nuclear factor-kappa B (NF-kB) , regulates the expressions of both COX-2 and dynorphin
Huang et al., Carcinogenesis 2004 (Neoplasm Invasiveness) : ICAM-1 promoter activities induced by the over-expression of wild-type NF-kappaB inducing kinase and IkappaB kinase beta (IKKbeta) were also inhibited by both compounds ... In summary, our data demonstrated that CYL-19 s and CYL-26z down-regulate the TNF-alpha induced inflammatory genes expression through suppression of IKK activity and NF-kappaB activation
Nishimura et al., Osteoarthritis Cartilage 2004 : ox-LDL binding to LOX-1 in BACs increased the production of intracellular ROS and activated NF-kappaB ... ox-LDL binding to LOX-1 in BACs increased the production of intracellular ROS and activated NF-kappaB ... ox-LDL binding to LOX-1 in BACs increased the production of intracellular ROS and activated NF-kappaB
Jiang et al., Life Sci 2004 : Effects of antioxidants and nitric oxide on TNF-alpha induced adhesion molecule expression and NF-kappaB activation in human dermal microvascular endothelial cells ... The mRNA expression of E-selectin, ICAM-1 and VCAM-1, and activation of NF-kappaB induced by TNF-alpha for 2 h were significantly decreased by the above two pretreatments ... To evaluate the role of antioxidants and nitric oxide in modulating inflammatory processes in the skin, we examined the effects of pyrrolidine dithiocarbamate ( PDTC, 0.1 mM ) and spermine NONOate ( Sper-NO, 1 mM ) on adhesion molecule expression and nuclear factor kappa B (NF-kappaB) activation induced by TNF-alpha ( 10 ng/ml ) in cultured human dermal microvascular endothelial cells ( HDMEC )
Watanabe et al., Nat Immunol 2004 (Crohn Disease) : Here we used Card15 ( -/- ) mice to show that intact NOD2 signaling inhibited Toll-like receptor 2-driven activation of NF-kappa B , particularly of the NF-kappa B subunit c-Rel ... Moreover, NOD2 deficiency or the presence of a Crohn disease-like Card15 mutation increased Toll-like receptor 2-mediated activation of NF-kappa B-c-Rel , and T ( H ) 1 responses were enhanced ... Moreover, NOD2 deficiency or the presence of a Crohn disease-like Card15 mutation increased Toll-like receptor 2-mediated activation of NF-kappa B-c-Rel , and T ( H ) 1 responses were enhanced
Melén et al., J Med Virol 2004 : Tumor necrosis factor-alpha (TNF-alpha) induced nuclear import of NF-kappaB was only weakly or not at all inhibited, suggesting that the nuclear import machinery in general was not impaired
Kim et al., Neuropharmacology 2004 : However, LPS induced MAP kinase or NF-kappa B activities were slightly or little changed by KL-1037
Kim et al., FEBS Lett 2004 : Furthermore, kahweol blocked the LPS induced activation of NF-kappaB by preventing IkappaB degradation and inhibiting IkappaB kinase activity
Kamezaki et al., Int Immunol 2004 (MAP Kinase Signaling System...) : The activation of MAPK and NF-kappaB by LPS , and the production of TNF-alpha and IL-12 after LPS injection, were not abrogated by the absence of Tyk2, Stat1 or Stat4
Jono et al., J Biol Chem 2004 (Inflammation) : NF-kappaB is essential for induction of CYLD , the negative regulator of NF-kappaB : evidence for a novel inducible autoregulatory feedback pathway ... The present study revealed a novel autoregulatory feedback pathway through which activation of NF-kappaB by TNF-alpha and bacterium nontypeable Haemophilus influenzae ( NTHi ) induces CYLD that in turn leads to the negative regulation of NF-kappaB signaling ... The present study revealed a novel autoregulatory feedback pathway through which activation of NF-kappaB by TNF-alpha and bacterium nontypeable Haemophilus influenzae ( NTHi ) induces CYLD that in turn leads to the negative regulation of NF-kappaB signaling ... In addition, TRAF2 and TRAF6 appear to be differentially involved in NF-kappaB dependent induction of CYLD by TNF-alpha and NTHi
Saccani et al., J Exp Med 2004 : Postinduction repression of NF-kappaB activity depends on NF-kappaB regulated resynthesis of IkappaBalpha , which dissociates NF-kappaB from DNA and exports it to the cytosol
Storz et al., Mol Pharmacol 2004 : Resveratrol, a potent antioxidant, blocks both PKD activation and NF-kappaB induction
Krappmann et al., Mol Cell Biol 2004 : The IkappaB kinase complex and NF-kappaB act as master regulators of lipopolysaccharide induced gene expression and control subordinate activation of AP-1 ... Thus, our data illustrate that NF-kappaB orchestrates immediate-early effects of LPS signaling and controls secondary AP-1 activation to mount an appropriate biological response
Reiterer et al., J Nutr 2004 (Inflammation) : However, if zinc was added back, all PPAR agonists significantly downregulated the TNF-alpha mediated induction of inflammatory transcription factors NF-kappaB and AP-1 and significantly reduced the expression of their target genes, VCAM-1 and IL-6
Hamilton et al., Arterioscler Thromb Vasc Biol 2004 : NF-kappaB mediates the early increase in HSP72, suggesting that estrogen activates NF-kappaB via a nongenomic, receptor dependent mechanism, and this leads to activation of HSF-1
Mishra et al., Arterioscler Thromb Vasc Biol 2004 : Using Western blot analyses, we show that the inhibition of NF-kappaB activation was not caused by prevention of phosphorylation of IkappaBalpha because oxidized EPA did not inhibit cytokine induced phosphorylation and ubiquination of IkappaBalpha ... Furthermore, oxidized EPA inhibited NF-kappaB activation in endothelial cells derived from wild-type mice but had no inhibitory effects on NF-kappaB activation in endothelial cells derived from peroxisome proliferator activated receptor alpha ( PPARalpha ) -deficient mice, indicating that oxidized EPA requires PPARalpha for its inhibitory effects on NF-kappaB
Zapolska-Downar et al., Life Sci 2004 : In TNFalpha stimulated HUVEC, simvastatin decreased VCAM-1 and ICAM-1 mRNA levels, inhibited TNFalpha induced activation of nuclear factor kappaB (NF-kappaB) and enhanced expression of peroxisome proliferator activated receptor alpha ( PPARalpha )
Hsieh et al., Cell Signal 2004 (MAP Kinase Signaling System) : In accordance with these findings, BK-induced phosphorylation of p42/p44 MAPK and Akt and activation of NF-kappaB was attenuated by prior treatment with PD98059, LY294002, and helenalin, respectively ... In accordance with these findings, BK-induced phosphorylation of p42/p44 MAPK and Akt and activation of NF-kappaB was attenuated by prior treatment with PD98059, LY294002, and helenalin, respectively
Jang et al., Biochem Biophys Res Commun 2004 (Adenocarcinoma) : Interestingly, IL-1beta induced nuclear factor-kappaB (NF-kappaB) activation in A549 cells, which was shown by increased nuclear translocation of p65 NF-kappaB and degradation of IkappaB-alpha ... Interestingly, IL-1beta induced nuclear factor-kappaB (NF-kappaB) activation in A549 cells, which was shown by increased nuclear translocation of p65 NF-kappaB and degradation of IkappaB-alpha ... Specifically, IL-1beta induced nuclear translocation of NF-kappaB was in part attenuated by LY294002, but not by GF109203X, SB203580, and SP600125, suggesting PI3K dependent nuclear translocation of NF-kappaB in response to IL-1beta ... Specifically, IL-1beta induced nuclear translocation of NF-kappaB was in part attenuated by LY294002, but not by GF109203X, SB203580, and SP600125, suggesting PI3K dependent nuclear translocation of NF-kappaB in response to IL-1beta ... Specifically, IL-1beta induced nuclear translocation of NF-kappaB was in part attenuated by LY294002, but not by GF109203X, SB203580, and SP600125, suggesting PI3K dependent nuclear translocation of NF-kappaB in response to IL-1beta
Tacchini et al., Carcinogenesis 2004 (Breast Neoplasms...) : Hepatocyte growth factor activated NF-kappaB regulates HIF-1 activity and ODC expression, implicated in survival, differently in different carcinoma cell lines ... Hepatocyte growth factor activated NF-kappaB regulates HIF-1 activity and ODC expression, implicated in survival, differently in different carcinoma cell lines
Le et al., J Immunol 2004 : These effects of TGF-beta1 culminate in the inhibition of LPS induced activation of NF-kappaB and the up-regulation of FPR2 in microglial cells ... These effects of TGF-beta1 culminate in the inhibition of LPS induced activation of NF-kappaB and the up-regulation of FPR2 in microglial cells
Takada et al., J Immunol 2004 : TNF activates Syk protein tyrosine kinase leading to TNF induced MAPK activation, NF-kappaB activation, and apoptosis ... Jurkat cells that did not express Syk ( JCaM1, JCaM1/lck ) showed lack of TNF induced Syk , JNK, p38 MAPK, and p44/p42 MAPK activation, as well as TNF induced IkappaBalpha phosphorylation, IkappaBalpha degradation, and NF-kappaB activation ... Jurkat cells that did not express Syk ( JCaM1, JCaM1/lck ) showed lack of TNF induced Syk, JNK, p38 MAPK, and p44/p42 MAPK activation, as well as TNF induced IkappaBalpha phosphorylation, IkappaBalpha degradation, and NF-kappaB activation ... TNF induced NF-kappaB activation was enhanced by overexpression of Syk by Syk-cDNA and suppressed when Syk expression was down-regulated by expression of Syk-small interfering RNA ( siRNA-Syk ) ... TNF induced NF-kappaB activation was enhanced by overexpression of Syk by Syk-cDNA and suppressed when Syk expression was down-regulated by expression of Syk-small interfering RNA ( siRNA-Syk ) ... The apoptotic effects of TNF were reduced by up-regulation of NF-kappaB by Syk-cDNA , and enhanced by down-regulation of NF-kappaB by siRNA-Syk ... Overall, our results demonstrate that Syk activation plays an essential role in TNF induced activation of JNK, p38 MAPK, p44/p42 MAPK, NF-kappaB , and apoptosis ... Overall, our results demonstrate that Syk activation plays an essential role in TNF induced activation of JNK, p38 MAPK, p44/p42 MAPK, NF-kappaB , and apoptosis
Platz et al., J Immunol 2004 : Up-regulation of NF-kappaB in epithelial cells in response to the CpG motif containing DNA was inhibited by overexpression of a dominant negative form of MyD88
Na et al., J Immunol 2004 (Arthritis, Experimental) : Inhibition of farnesyltransferase prevents collagen induced arthritis by down-regulation of inflammatory gene expression through suppression of p21(ras) dependent NF-kappaB activation ... Together, these findings reveal that the inhibitory effect of LB42708 on p21(ras) dependent NF-kappaB activation may have potential therapeutic value for arthritis and other inflammatory diseases
Ishihara et al., J Immunol 2004 (Gastritis...) : NF-kappaB activation and activation of IL-8 and MD-2 promoters were assessed by reporter gene assay ... MD-2 transfection in AGS cells markedly increased cell surface expression of TLR4 and augmented the activation of NF-kappaB and IL-8 promoter upon stimulation with H. pylori LPS
Zelivianski et al., Nucleic Acids Res 2004 (Prostatic Neoplasms) : Transcriptional activation of the human prostatic acid phosphatase gene by NF-kappaB via a novel hexanucleotide binding site
Rigas et al., Trends Mol Med 2004 (Neoplasms) : Mechanistically, NO-aspirin, the best studied NO-NSAID, has pleiotropic effects on cell signaling ( it inhibits Wnt signaling, induces nitric oxide synthase and NF-kappaB activation and induces cyclooxygenase-2 expression ), and this mechanistic redundancy might be central to its mode of action against cancer ... Mechanistically, NO-aspirin, the best studied NO-NSAID, has pleiotropic effects on cell signaling ( it inhibits Wnt signaling, induces nitric oxide synthase and NF-kappaB activation and induces cyclooxygenase-2 expression ), and this mechanistic redundancy might be central to its mode of action against cancer
Ogawa et al., Am J Physiol Gastrointest Liver Physiol 2004 (Colitis) : Consistent with its upregulated expression by proinflammatory stimuli, CCL28 mRNA expression was attenuated by pharmacological inhibitors of NF-kappaB activation ... Consistent with its upregulated expression by proinflammatory stimuli, CCL28 mRNA expression was attenuated by pharmacological inhibitors of NF-kappaB activation
Rahman et al., Am J Physiol Lung Cell Mol Physiol 2004 : This effect of cAMP was secondary to inhibition of NF-kappaB activity, the key regulator of thrombin induced ICAM-1 expression in endothelial cells ... Our data demonstrate that increased intracellular cAMP concentration in endothelial cells prevents thrombin induced ICAM-1 expression by inhibiting p38 MAPK activation, which in turn prevents phosphorylation of RelA/p65 and transcriptional activity of the bound NF-kappaB
Rangaswami et al., J Biol Chem 2004 (MAP Kinase Signaling System...) : OPN induced NIK dependent NFkappaB activation through ERK/IKKalpha/beta mediated pathways ... Wild type NIK, IKKalpha/beta, and ERK1/2 enhanced and kinase negative NIK ( mut NIK ), dominant negative IKKalpha/beta ( dn IKKalpha/beta ), and dn ERK1/2 suppressed the OPN induced NFkappaB activation, uPA secretion, pro-MMP-9 activation, cell motility, and chemoinvasion ... Wild type NIK, IKKalpha/beta, and ERK1/2 enhanced and kinase negative NIK ( mut NIK ), dominant negative IKKalpha/beta ( dn IKKalpha/beta ), and dn ERK1/2 suppressed the OPN induced NFkappaB activation, uPA secretion, pro-MMP-9 activation, cell motility, and chemoinvasion ... Wild type NIK, IKKalpha/beta, and ERK1/2 enhanced and kinase negative NIK ( mut NIK ), dominant negative IKKalpha/beta ( dn IKKalpha/beta ), and dn ERK1/2 suppressed the OPN induced NFkappaB activation , uPA secretion, pro-MMP-9 activation, cell motility, and chemoinvasion ... To our knowledge, this is the first report that NIK plays a crucial role in OPN induced NFkappaB activation , uPA secretion, and pro-MMP-9 activation through MAPK/IKKalpha/beta mediated pathways, and all of these ultimately control the cell motility, invasiveness, and tumor growth
Guo et al., Nat Genet 2004 (Diabetes Mellitus, Type 1...) : SUMO4 conjugates to I kappa B alpha and negatively regulates NF kappa B transcriptional activity
Harigai et al., Arthritis Rheum 2004 (Arthritis, Rheumatoid...) : Stimulation with rsCD154 plus IFNgamma induced the activation of ERK-1/2, p38 MAPK, and NF-kappaB
Coward et al., Clin Exp Allergy 2004 : The NF-kappaB inhibitors, calpain inhibitor 1 ( 10 microm ), pentoxifylline ( 0.5 mm ), pyrrolidine dithiocarbamate ( PDTC, 10 microm ) or gliotoxin ( 1 pg/mL ) reduced the generation of GM-CSF, TNF-alpha and IL-8 in parallel with their inhibition of NF-kappaB ... The NF-kappaB inhibitors, calpain inhibitor 1 ( 10 microm ), pentoxifylline ( 0.5 mm ), pyrrolidine dithiocarbamate ( PDTC, 10 microm ) or gliotoxin ( 1 pg/mL ) reduced the generation of GM-CSF , TNF-alpha and IL-8 in parallel with their inhibition of NF-kappaB ... The NF-kappaB inhibitors, calpain inhibitor 1 ( 10 microm ), pentoxifylline ( 0.5 mm ), pyrrolidine dithiocarbamate ( PDTC, 10 microm ) or gliotoxin ( 1 pg/mL ) reduced the generation of GM-CSF, TNF-alpha and IL-8 in parallel with their inhibition of NF-kappaB
Lee et al., Biochem Biophys Res Commun 2004 : EPO receptor mediated ERK kinase and NF-kappaB activation in erythropoietin promoted differentiation of astrocytes ... However, in the presence of PD98,059, an inhibitor of ERK , and salicylic acid, an NF-kappaB inhibitor, the EPO induced morphological differentiation of astrocytes and expression of FGAP and EPO receptor were reduced ... Conversely, treatment with anti-EPO and EPO receptor antibodies also reduced EPO induced ERK ( 2 ) and NF-kappaB activation
Cai et al., Circulation 2004 (Cardiovascular Diseases...) : In addition, Db-HAGE-LDL stimulated NF-kappaB activity significantly in ECV 304 and human umbilical vein endothelial cells ( 2.3-fold above baseline ) in a manner inhibitable by a MEK inhibitor PD98059 ( 10 micromol/L ), the antioxidant N-acetyl-l-cysteine, NAC ( 30 mmol/L ), and the NADPH oxidase inhibitor DPI ( 20 micromol/L )
Zhang et al., FEBS Lett 2004 : PIASy represses TRIF induced ISRE and NF-kappaB activation but not apoptosis ... In reporter gene assays, PIASy dramatically inhibits TRIF induced NF-kappaB , ISRE and IFN-beta activation but not TRIF induced apoptosis
Hoentjen et al., Blood 2005 : STAT3 regulates NF-kappaB recruitment to the IL-12p40 promoter in dendritic cells ... In this study, we investigated the role of nuclear factor-kappa B (NF-kappaB) and signal transducers and activators of transcription 3 ( STAT3 ) in lipopolysaccharide (LPS) induced IL-12p40 gene expression in bone marrow derived-dendritic cells ( BMDCs ) isolated from wild-type ( WT ) and IL-10 ( -/- ) mice ... In this study, we investigated the role of nuclear factor-kappa B (NF-kappaB) and signal transducers and activators of transcription 3 ( STAT3 ) in lipopolysaccharide (LPS) induced IL-12p40 gene expression in bone marrow derived-dendritic cells ( BMDCs ) isolated from wild-type ( WT ) and IL-10 ( -/- ) mice ... LPS induced NF-kappaB signaling is similar in IL-10 ( -/- ) and WT BMDCs as measured by IkappaBalpha phosphorylation and degradation, RelA phosphorylation and nuclear translocation, and NF-kappaB transcriptional activity, with no down-regulatory effects of exogenous IL-10 ... Chromatin immunoprecipitation demonstrated enhanced NF-kappaB ( cRel, RelA ) binding to the IL-12p40 promoter in IL-10 ( -/- ) but not WT BMDCs. Interestingly, LPS induced STAT3 phosphorylation in WT but not IL-10 ( -/- ) BMDCs, a process blocked by IL-10 receptor blocking antibody ... Chromatin immunoprecipitation demonstrated enhanced NF-kappaB ( cRel, RelA ) binding to the IL-12p40 promoter in IL-10 ( -/- ) but not WT BMDCs. Interestingly, LPS induced STAT3 phosphorylation in WT but not IL-10 ( -/- ) BMDCs, a process blocked by IL-10 receptor blocking antibody ... Chromatin immunoprecipitation demonstrated enhanced NF-kappaB ( cRel, RelA ) binding to the IL-12p40 promoter in IL-10 ( -/- ) but not WT BMDCs. Interestingly, LPS induced STAT3 phosphorylation in WT but not IL-10 ( -/- ) BMDCs, a process blocked by IL-10 receptor blocking antibody ... In conclusion, dysregulated LPS induced IL-12p40 gene expression in IL-10 ( -/- ) mice is due to enhanced NF-kappaB recruitment to the IL-12p40 promoter in the absence of activated STAT3 ... In conclusion, dysregulated LPS induced IL-12p40 gene expression in IL-10 ( -/- ) mice is due to enhanced NF-kappaB recruitment to the IL-12p40 promoter in the absence of activated STAT3
Sinha et al., J Biol Chem 2004 (Insulin Resistance) : Supporting the hypothesis that IKK acts through NFkappaB to cause insulin resistance, the IKK inhibitors acetylsalicylate and parthenolide prevented FA-induced reductions in insulin stimulated glucose uptake and NFkappaB nuclear translocation ... Supporting the hypothesis that IKK acts through NFkappaB to cause insulin resistance, the IKK inhibitors acetylsalicylate and parthenolide prevented FA-induced reductions in insulin stimulated glucose uptake and NFkappaB nuclear translocation ... We conclude that FAs induce insulin resistance and activates NFkappaB in L6 cells ... Furthermore, inhibition of NFkappaB activation, indirectly by preventing IKK activation or directly by inhibiting NFkappaB nuclear translocation, prevents the detrimental effects of palmitate on the metabolic actions of insulin in L6 myotubes
Rathore et al., J Biol Chem 2004 : The late phase of the TRAIL induced NF-kappaB is critically dependent on caspase 8 and can be blocked by pharmacological and genetic inhibitors of caspase 8 activation, such as benzyloxycarbonyl-VAD-fluoromethyl ketone, benzyloxycarbonyl-IETD-fluoromethyl ketone, and small interfering RNA targeting caspase 8 and FADD ... Whereas caspase 3 is required for TRAIL induced apoptosis, it is not involved in TRAIL induced NF-kappaB activation ... The late phase of TRAIL induced NF-kappaB activation involves caspase mediated cleavage of IkappaBalpha between Asp ( 31 ) and Ser ( 32 ) residues to generate an N-terminal truncated fragment that is degraded by the proteasome via the N-end rule pathway
Takada et al., J Biol Chem 2004 : TNF induced NF-kappaB activation as measured by DNA binding in wild-type mouse embryonic fibroblasts, but deletion of GSK-3beta abolished this activation ... TNF induced NF-kappaB reporter gene transcription was also suppressed in GSK-3beta gene deleted cells
Deeb et al., Mol Cancer Ther 2004 (Prostatic Neoplasms) : A similar increase in the sensitivity of LNCaP cells to TRAIL induced apoptosis was observed following inhibition of NF-kappaB by dominant negative mutant IkappaBalpha , an inhibitor of NF-kappaB ... Finally, curcumin was found to inhibit NF-kappaB by blocking phosphorylation of IkappaBalpha ... We conclude that NF-kappaB mediates resistance of LNCaP cells to TRAIL and that curcumin enhances the sensitivity of these tumor cells to TRAIL by inhibiting NF-kappaB activation by blocking phosphorylation of IkappaBalpha and its degradation
Aggarwal et al., Int J Cancer 2004 (Carcinoma, Squamous Cell...) : Our results indicate that curcumin is a potent inhibitor of cell proliferation and an inducer of apoptosis in HNSCC through suppression of IKK mediated NF-kappaB activation and of NF-kappaB regulated gene expression
Shinohara et al., Int J Mol Med 2004 : SEB and IFN-gamma activated NF-kappaB , which was inhibited by PDTC ... SEB and IFN-gamma activated NF-kappaB , which was inhibited by PDTC
Shukla et al., Neoplasia (New York, N.Y.) 2004 (Adenocarcinoma...) : The nuclear levels of NF-kappaB/p65 correlated with concurrent increase in cytosolic levels of IkappaBalpha along with NF-kappaB dependent expression of Bcl2, cyclin D1, MMP-9, and VEGF ... The nuclear levels of NF-kappaB/p65 correlated with concurrent increase in cytosolic levels of IkappaBalpha along with NF-kappaB dependent expression of Bcl2, cyclin D1, MMP-9 , and VEGF ... The nuclear levels of NF-kappaB/p65 correlated with concurrent increase in cytosolic levels of IkappaBalpha along with NF-kappaB dependent expression of Bcl2, cyclin D1 , MMP-9, and VEGF ... The nuclear levels of NF-kappaB/p65 correlated with concurrent increase in cytosolic levels of IkappaBalpha along with NF-kappaB dependent expression of Bcl2, cyclin D1, MMP-9 , and VEGF ... The nuclear levels of NF-kappaB/p65 correlated with concurrent increase in cytosolic levels of IkappaBalpha along with NF-kappaB dependent expression of Bcl2 , cyclin D1, MMP-9, and VEGF
Lee et al., Free Radic Biol Med 2004 : These findings strongly suggest that in endothelial cells, HHE induces iNOS gene expression through NF-kappaB activation, which can lead to vascular dysfunction by the activation of various proinflammatory genes
Bobrovnikova-Marjon et al., Cancer Res 2004 (Breast Neoplasms) : Blocking NFkappaB and AP-1 activation with curcumin as well as expression of dominant inhibitors, inhibitor of nuclear factor-kappaB ( IkappaB ) super repressor ( IkappaBM ), and a mutant form of c-Fos ( A-Fos ) demonstrated that the activation of NFkappaB and AP-1 transcription factors was necessary for the induction of IL-8 expression but dispensable for the induction of VEGF expression
Shishodia et al., Cancer Res 2004 (Carcinoma, Non-Small-Cell Lung...) : CSC induced NF-kappaB reporter activity induced by NF-kappaB inducing kinase and IkappaB alpha kinase but not that activated by p65 was also blocked by celecoxib ... CSC induced NF-kappaB reporter activity induced by NF-kappaB inducing kinase and IkappaB alpha kinase but not that activated by p65 was also blocked by celecoxib
Zhang et al., Carcinogenesis 2004 (Breast Neoplasms) : Such sensitization is closely associated with the inhibitory effect of PN on TNF-alpha mediated NF-kappaB activation ... Our study revealed a new mechanism that PN inhibits TNF-alpha mediated NF-kappaB activation via disrupting the recruitment of the IkappaB kinases (IKK) complex to TNF receptor, which then blocked the subsequent signaling events including IKK kinase activation, IkappaBalpha degradation, p65 nuclear translocation, DNA binding and transactivation
Chen et al., J Cell Biochem 2004 (Carcinoma, Papillary...) : Furthermore, we demonstrated that increased levels of HO-1 and p21 expression are associated with an increase in the activity of NF-kappaB and that inhibiting NF-kappaB leads to a block in the induction of HO-1 and p21 ... Furthermore, we demonstrated that increased levels of HO-1 and p21 expression are associated with an increase in the activity of NF-kappaB and that inhibiting NF-kappaB leads to a block in the induction of HO-1 and p21
Seo et al., Eur J Immunol 2004 (Neovascularization, Pathologic) : These data indicate that PAF induced NF-kappaB activation is a common upstream pathway leading to the production of crucial macrophage derived angiogenic factors
Hamanoue et al., Neurochem Res 2004 : This anti-apoptotic activity was repressed by IkappaB-alpha , an inhibitor of NF-kappaB
Mazzeo et al., Arch Neurol 2004 (Amyloid Neuropathies, Familial...) : Our novel findings suggest a crucial role of NF-kappaB in inflammatory neuropathies and FAP
Pawate et al., J Neurosci Res 2004 (Encephalitis...) : Phox inhibitors and catalase also suppressed LPS/IFNgamma induced expression of cytokines, i.e., interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)alpha and blocked LPS activation of MAP kinases ( i.e., p38 MAPK, c-Jun N-terminal kinase and extracellular signal regulated kinase ), NFkappaB , and IFNgamma induced STAT1 phosphorylation ... Phox inhibitors and catalase also suppressed LPS/IFNgamma induced expression of cytokines, i.e., interleukin (IL)-1, IL-6, and tumor necrosis factor (TNF)alpha and blocked LPS activation of MAP kinases ( i.e., p38 MAPK, c-Jun N-terminal kinase and extracellular signal regulated kinase ), NFkappaB , and IFNgamma induced STAT1 phosphorylation
Cao et al., Chin Med J (Engl) 2004 : TNF-alpha induced NF-kappaB activation was measured by electrophoretic mobility shift assay ( EMSA ) ... The results of EMSA showed that NF-kappaB activation by TNF-alpha in HL-60 cells was induced in a dose dependent manner, but was almost completely inhibited by mutant IkappaBalpha repressor in transfected cells
Zhao et al., J Neuroimmunol 2004 (MAP Kinase Signaling System) : 15d-PGJ2 inhibited transactivation of NF-kappaB dependent promoters, as well as p38 and JNK MAPK phosphorylation induced by IL-1, while having no inhibitory effect on IFN induced Stat signaling pathways ... 15d-PGJ2 inhibited transactivation of NF-kappaB dependent promoters, as well as p38 and JNK MAPK phosphorylation induced by IL-1, while having no inhibitory effect on IFN induced Stat signaling pathways
Dai et al., J Immunol 2004 : In this study, we demonstrate that both LPS and HSV up-regulate the expression of IRF-7 in PDC, and that this enhancement of IRF-7 is dependent on NF-kappa B activation ... In this study, we demonstrate that both LPS and HSV up-regulate the expression of IRF-7 in PDC, and that this enhancement of IRF-7 is dependent on NF-kappa B activation
Shishodia et al., J Immunol 2004 (Carcinoma, Non-Small-Cell Lung...) : Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma : correlation with suppression of COX-2 synthesis ... Akt activation, which is required for TNF induced NF-kappa B activation, was also suppressed by this drug
Xie et al., J Biol Chem 2004 : Inhibition of ERK1/2, JNKs, and PKC-alpha/beta1 had no effect on NF-kappaB activation, whereas inhibition of PKC-theta and PKC-zeta inhibited IL-1beta stimulated activation of NF-kappaB ... These observations suggest that 1 ) activation of ERK1/2 and JNKs plays a critical role in the regulation of MMP-9, not MMP-2, expression and activity ; 2 ) PKC-alpha/beta1 act upstream of JNKs, not ERK1/2 ; 3 ) PKC-zeta and -theta, not PKC-epsilon, act upstream of JNKs, ERK1/2, and NF-kappaB ; and 4 ) activation of NF-kappaB stimulates expression and activity of MMP-2 and -9 ... These observations suggest that 1 ) activation of ERK1/2 and JNKs plays a critical role in the regulation of MMP-9, not MMP-2, expression and activity ; 2 ) PKC-alpha/beta1 act upstream of JNKs, not ERK1/2 ; 3 ) PKC-zeta and -theta, not PKC-epsilon, act upstream of JNKs, ERK1/2, and NF-kappaB ; and 4 ) activation of NF-kappaB stimulates expression and activity of MMP-2 and -9
Shephard et al., Thromb Haemost 2004 : GM-CSF activated NFkappaB , thus interfering with TGF-beta signaling
Khan et al., Immunology 2004 : Finally, we demonstrated that pharmacological inhibition of p38 MAPK reduced IL-8 transcription and mRNA levels, but did not affect NF-kappaB activation
Chambers et al., Clin Exp Immunol 2004 (HIV Infections) : HIV infection of THP-1 myeloid cells resulted in decreased LPS induced nuclear factor binding to the NF-kappaB , AP-1, and Sp1 sites of the IL-12 p40 promoter
Kitagawa et al., Am J Physiol Gastrointest Liver Physiol 2004 : IL-1beta stimulated the degradation of IkappaB and the activation of NF-kappaB but had no effect on iNOS induction ... EGF, which had no effect on the NF-kappaB activation and iNOS induction , stimulated the upregulation of type 1 IL-1 receptor ( IL-1R1 ) through PI3-kinase/Akt ... Our results indicate that EGF and IL-1beta stimulate two essential signals for iNOS induction in IEC-6 cells : the upregulation of IL-1R1 through PI3-kinase/Akt and the activation of NF-kappaB through IkappaB kinase, respectively ... Our results indicate that EGF and IL-1beta stimulate two essential signals for iNOS induction in IEC-6 cells : the upregulation of IL-1R1 through PI3-kinase/Akt and the activation of NF-kappaB through IkappaB kinase , respectively ... Our results indicate that EGF and IL-1beta stimulate two essential signals for iNOS induction in IEC-6 cells : the upregulation of IL-1R1 through PI3-kinase/Akt and the activation of NF-kappaB through IkappaB kinase, respectively ... Our results indicate that EGF and IL-1beta stimulate two essential signals for iNOS induction in IEC-6 cells : the upregulation of IL-1R1 through PI3-kinase/Akt and the activation of NF-kappaB through IkappaB kinase, respectively
Huang et al., Arterioscler Thromb Vasc Biol 2004 : Furthermore, LTB4 increased NF-[kappa]B DNA binding activity, which was blocked by CP-105,696 ... The NF-[kappa]B activation may be involved in LTB4 increased MCP-1 expression
Yamagishi et al., J Mol Cell Cardiol 2004 : Accordingly, we investigated effects of PEDF on reactive oxygen species ( ROS ) generation, NF-kappaB activation and interleukin (IL)-6 expression in TNF-alpha exposed HUVEC ... PEDF or an antioxidant, N-acetylcysteine, significantly inhibited the TNF-alpha induced NF-kappaB activation ... PEDF or an antioxidant, N-acetylcysteine, significantly inhibited the TNF-alpha induced NF-kappaB activation ... The results demonstrated that PEDF inhibited TNF-alpha induced NF-kappaB activation and subsequent IL-6 overexpression in HUVEC by suppressing NADPH oxidase mediated ROS generation ... The results demonstrated that PEDF inhibited TNF-alpha induced NF-kappaB activation and subsequent IL-6 overexpression in HUVEC by suppressing NADPH oxidase mediated ROS generation ... The results demonstrated that PEDF inhibited TNF-alpha induced NF-kappaB activation and subsequent IL-6 overexpression in HUVEC by suppressing NADPH oxidase mediated ROS generation
Palamakumbura et al., J Biol Chem 2004 : Here we report, for the first time, that the lysyl oxidase propeptide, and not the lysyl oxidase enzyme, inhibits ras dependent transformation as determined by effects on cell proliferation assays, growth in soft agar, and Akt dependent induction of NF-kappaB activity
Shin et al., FEBS Lett 2004 (Inflammation) : The aromatic diamine JSH-23 compound exhibited inhibitory effect with an IC ( 50 ) value of 7.1 microM on nuclear factor (NF)-kappaB transcriptional activity in lipopolysaccharide (LPS) stimulated macrophages RAW 264.7, and interfered LPS induced nuclear translocation of NF-kappaB without affecting IkappaB degradation
Cummings et al., J Biol Chem 2004 (Inflammation) : Here, we report that protein kinase Cdelta (PKCdelta) mediates LPA induced NF-kappaB transcription and interleukin-8 (IL-8) secretion in HBEpCs ... Here, we report that protein kinase Cdelta (PKCdelta) mediates LPA induced NF-kappaB transcription and interleukin-8 (IL-8) secretion in HBEpCs ... LPA caused a marked activation of NF-kappaB in HBEpCs as determined by IkappaB phosphorylation and of NF-kappaB nuclear translocation and a strong induction of NF-kappaB promoter mediated luciferase activity ... Furthermore, LPA activated PKCdelta and the LPA mediated activation of NF-kappaB and IL-8 production were attenuated by overexpression of dominant negative PKCdelta and rottlerin
Ramana et al., FASEB J 2004 (Inflammation) : Inhibition of AR abrogated TNF-alpha induced activation and membrane translocation of PKC, and antisense ablation of AR prevented both TNF-alpha induced PKC and NF-kappaB activation
Xu et al., World J Gastroenterol 2004 : GM-CSF+IL-4 propagated DCs displayed features of mature DCs, with high levels of costimulatory molecule ( CD40, CD80, CD86 ) surface expression, marked NF-kappaB activation , and significant allocostimulatory activity ... NF-kappaB decoy ODNs completely abrogated IL-4 induced DC maturation and allocostimulatory activity as well as LPS induced NF-kappaB activation and IL-12 protein expression in DCs ... NF-kappaB decoy ODNs completely abrogated IL-4 induced DC maturation and allocostimulatory activity as well as LPS induced NF-kappaB activation and IL-12 protein expression in DCs
Magnenat et al., J Mol Biol 2004 : We show that a TFZF that interacts directly with the ICAM-1 promoter at an overlapping NF-kappaB binding enhancer can overcome or synergistically cooperate with NF-kappaB induction of ICAM-1
Kreuz et al., J Cell Biol 2004 : NFkappaB activation by Fas is mediated through FADD, caspase-8, and RIP and is inhibited by FLIP ... Remarkably, the enzymatic activity of the latter was dispensable for Fas induced NFkappaB signaling pointing to a scaffolding related function of caspase-8 in nonapoptotic Fas signaling ... However, in the context of Fas signaling both isoforms blocked FasL induced NFkappaB activation ... As NFkappaB signaling is inhibited during apoptosis, FasL induced NFkappaB activation was most prominent in cells that were protected by Bcl2 expression or caspase inhibitors and expressed no or minute amounts of FLIP
Lu et al., Proc Natl Acad Sci U S A 2004 (Inflammation) : However, AGE stimulated NF-kappaB activity and mitogen activated protein kinase ( MAPK ) ( p44/42 ) phosphorylation were found markedly suppressed in R1-MC. Additionally, AGE stimulated macrophage chemotaxis protein 1 and RAGE overexpression were abolished in R1-MC
McEleny et al., BJU Int 2004 (Prostatic Neoplasms) : CAPE prevented paclitaxel and TNFalpha mediated NF-kappaB activation
Fisher et al., Gene Ther 2004 : The ability of the transport peptide-PNA-NFkappaB decoy complex to block the effect of interleukin (IL)-1beta induced NFkappaB activation and IL-6 gene expression was analyzed by electrophoretic mobility shift assay and reverse transcriptase-polymerase chain reaction in rat Rinm5F insulinoma cells ... The ability of the transport peptide-PNA-NFkappaB decoy complex to block the effect of interleukin (IL)-1beta induced NFkappaB activation and IL-6 gene expression was analyzed by electrophoretic mobility shift assay and reverse transcriptase-polymerase chain reaction in rat Rinm5F insulinoma cells ... Preincubation with transport peptide-PNA-NFkappaB decoy ( 1 microM, 1 h ) blocked IL-1beta induced NFkappaB binding activity and significantly reduced the IL-6 mRNA expression
Jiang et al., Free radical research 2004 : This study also addresses PDTC and Sper-NO effects on activation of nuclear factor kappa B (NF-kappaB) induced by TNF-alpha ( 10 ng/ml ) ... The mRNA accumulation of IL-8, MCP-1, RANTES, and eotaxin, and activation of NF-kappaB were induced by TNF-alpha for 2 h ; all were suppressed significantly by the above two pretreatments
Giarratana et al., J Immunol 2004 (Diabetes Mellitus, Type 1) : The inhibition of islet chemokine production in vivo persists after restimulation with TLR ligands and is associated with up-regulation of IkappaBalpha transcription, an inhibitor of NF-kappaB and with arrest of NF-kappaBp65 nuclear translocation, highlighting a novel mechanism of action exerted by vitamin D receptor ligands potentially relevant for the treatment of T1D and other autoimmune diseases
Falsig et al., J Immunol 2004 (Inflammation) : Although CEP-1347 did not affect the activation of NF-kappaB , it blocked the expression of cyclooxygenase-2 and inducible NO synthase at the transcriptional level
Teague et al., J Interferon Cytokine Res 2004 : Previously, we demonstrated that treatment of B cells with MIP-1alpha induced the transcription factor, nuclear factor (NF)-kappaB, to bind to DNA, concomitant with the degradation of IkappaBalpha , a cytoplasmic inhibitor of NF-kappaB activation
Kharroubi et al., Endocrinology 2004 (Insulinoma...) : Moreover, FFAs did not enhance NF-kappaB activation by TNFalpha
Rundall et al., Surgery 2004 (Carcinoma, Non-Small-Cell Lung...) : NF-kappaB activity was determined by reporter-gene assays and by reverse transcriptase-polymerase chain reaction of the endogenous NF-kappaB dependent gene interleukin 8
Khadaroo et al., Surgery 2004 (Respiratory Distress Syndrome, Adult...) : We have shown that oxidant stress generated by hemorrhagic shock/resuscitation ( S/R ) in rodents increases lipopolysaccharide (LPS) induced lung injury and translocation of nuclear factor kappa B (NF-kappaB) in alveolar macrophages ( AMs ) ... The LPS induced NF-kappaB translocation in AMs after shock/resuscitation was 3-fold higher than in sham AMs treated with LPS
Trevisan et al., Exp Cell Res 2004 : As Tax can enhance the transcriptional activity of nuclear factor NF-kB and cAMP-responsive element binding protein/activating transcription factor-1 ( CREB/ATF-1 ), we investigated the relevance of these routes in the anti-apoptotic effects of Tax
Xue et al., Exp Cell Res 2004 : APC also significantly increased the production of IL-6 and IL-8 and suppressed calcium- and LPS stimulated NF-kappaB activity ... APC also significantly increased the production of IL-6 and IL-8 and suppressed calcium- and LPS stimulated NF-kappaB activity
Wiener et al., Exp Cell Res 2004 (Carcinoma, Non-Small-Cell Lung...) : Synergistic induction of the Fas ( CD95) ligand promoter by Max and NFkappaB in human non-small lung cancer cells ... In contrast, we observed that overexpression of Max resulted in a marked increase in basal promoter activity and synergistically enhanced phorbolester- and doxorubicin induced NFkappaB mediated Fas ligand promoter activity ... We conclude that high levels of Max and stress induced NFkappaB activation may result in elevated expression of Fas ligand in human lung cancer cells and possibly contribute to Fas ligand associated immune escape mechanisms
Nakajima et al., J Biol Chem 2004 (Inflammation) : Electrophoretic mobility shift assay revealed that PARP-1 inhibitors and the antisense RNA for PARP-1 mRNA reduced the LPS induced DNA binding of NF-kappaB ... Electrophoretic mobility shift assay revealed that PARP-1 inhibitors and the antisense RNA for PARP-1 mRNA reduced the LPS induced DNA binding of NF-kappaB ... Non modified PARP-1 also reduced the DNA binding of NF-kappaB via its physical association with NF-kappaB, whereas AM-PARP had no effect
Kaneko et al., Biol Pharm Bull 2004 : Manganese superoxide dismutase is induced by endoplasmic reticulum stress through IRE1 mediated nuclear factor (NF)-kappaB and AP-1 activation ... These results suggest that the MnSOD expression is controlled by ER stress through IRE1 mediated NF-kappaB and AP-1 activation
Dickson et al., Dev Dyn 2004 : TRAF6 dependent NF-kB transcriptional activity during mouse development ... To assess the role of the TRAF6 adaptor protein in developmental NF-kB activity, we analyzed NF-kB activation in reporter mice rendered null for TRAF6
Su et al., Immunity 2004 (Lymphoproliferative Disorders) : Another fork in the road : Foxo3a regulates NF-kappaB activation
Lin et al., Immunity 2004 (Hypersensitivity...) : Regulation of NF-kappaB , Th activation, and autoinflammation by the forkhead transcription factor Foxo3a ... Foxo3a inhibits NF-kappaB activation, whose overactivity was responsible for T cell hyperactivity in Foxo3a-deficient mice ... Thus, Foxo3a regulates helper T cell activation and tolerance by inhibiting NF-kappaB activity, reinforcing a generalized role for the forkhead proteins in the maintenance of T cell tolerance through the inhibition of inflammatory transcriptional activities
Beak et al., Biochimie 2004 : These results suggest that the NADPH oxidase and COX may be major sources for the UVB induced ROS generation, and play an essential role in the activation of NF-kappaB which is involved in the expression of a variety of genes induced by UVB in HaCaT cells ... These results suggest that the NADPH oxidase and COX may be major sources for the UVB induced ROS generation, and play an essential role in the activation of NF-kappaB which is involved in the expression of a variety of genes induced by UVB in HaCaT cells
Abid et al., J Biol Chem 2004 : VEGF resulted in protein kinase C-dependent phosphorylation of IkappaB and subsequent translocation of p65 NF-kappaB into the nucleus
Wenk et al., J Biol Chem 2004 : As interleukin-6 (IL-6) was earlier shown to mediate the UVA induction of MMP-1, we studied whether PHGPx overexpression might interfere with the NFkappaB mediated IL-6 induction and downstream signaling ... This together with the suppression of UVA irradiation induced IL-6 release in the presence of Trolox, a chain breaker of PCOOH initiated lipid peroxidation, indicates that UVA irradiation induced PCOOHs and subsequent lipid peroxides initiate the NFkappaB mediated induction of IL-6 , which mediates the induction of MMP-1
Venkatesha et al., J Biol Chem 2004 : Interestingly, PAF induced significantly higher NF-kappaB and nuclear factor of activated T cells ( NFAT ) -luciferase activity as well as CCL2 production in cells expressing DeltaST-PAFR than those expressing PAFR ... Furthermore, a Ca2+/calcineurin inhibitor completely inhibited PAF induced NFAT activation and CCL2 production but not NF-kappaB activation ... Furthermore, a Ca2+/calcineurin inhibitor completely inhibited PAF induced NFAT activation and CCL2 production but not NF-kappaB activation ... Furthermore, a Ca2+/calcineurin inhibitor completely inhibited PAF induced NFAT activation and CCL2 production but not NF-kappaB activation
Xiong et al., J Biol Chem 2004 : Interaction of TRAF6 with MAST205 regulates NF-kappaB activation and MAST205 stability ... Interaction of TRAF6 with MAST205 regulates NF-kappaB activation and MAST205 stability
Guo et al., J Virol 2004 (Cell Transformation, Neoplastic...) : The HHV-8 chemokine receptor, vGPCR ( ORF74 ), activates NF-kappaB and NF-AT, and their levels of activation are synergistically increased by HIV-1 Tat
Zhang et al., J Biol Chem 2004 : At the same time, the binding of AIP1 to TRAF2 inhibits TNF induced IKK-NF-kappaB signaling ... At the same time, the binding of AIP1 to TRAF2 inhibits TNF induced IKK-NF-kappaB signaling ... At the same time, the binding of AIP1 to TRAF2 inhibits TNF induced IKK-NF-kappaB signaling
Cuschieri et al., J Surg Res 2004 : Subsequently, LPS induced the activation of NF-kappaB and AP-1
Demonté et al., Biochem Pharmacol 2004 (HIV Infections) : In this context, we have previously demonstrated that deacetylase inhibitors ( HDACi ) synergize with TNF induced NF-kappaB to activate the HIV-1 promoter
Fujioka et al., Mol Cell Biol 2004 : NF-kappaB and AP-1 connection : mechanism of NF-kappaB dependent regulation of AP-1 activity ... Thus, our study revealed a distinct and essential role of NF-kappaB in participating in the regulation of elk-1, c-fos, and VEGF expression ... Thus, our study revealed a distinct and essential role of NF-kappaB in participating in the regulation of elk-1 , c-fos, and VEGF expression
Chen et al., Apoptosis 2004 (Colonic Neoplasms) : Bcl-2, and to the less extent, Bcl-xL , were able to increase the activity of NF-kappa B , which was a known anti-apoptotic molecule in human colon cancer cells
Ikeda et al., J Clin Invest 2004 : Although genetic and biochemical studies indicate that RANKL regulates osteoclast differentiation by activating receptor activator of NF-kappaB and associated signaling molecules, the molecular mechanisms of RANKL regulated osteoclast differentiation have not yet been fully established
Huber et al., J Clin Invest 2004 (Breast Neoplasms...) : Inhibition of NF-kappaB signaling prevented EMT in Ras transformed epithelial cells, while activation of this pathway promoted the transition to a mesenchymal phenotype even in the absence of TGF-beta
Zheng et al., J Huazhong Univ Sci Technolog Med Sci 2004 (Pancreatitis) : The activity of NF-kappaB in pancreatic acinar cells was significantly increased ( P < 0.01 ) after treated with M3 cholinergic receptor agonist ( 10 ( -3 ) mol/L carbachol ) in vitro for 30 min. Either M3 cholinergic receptor antagonist ( 10 ( -5 ) mol/L atropine ) or NF-kappaB inhibitor ( 10 ( -2 ) mol/L PDTC ) could obviously inhibit the activation of NF-kappaB and the chemokine MOB-1, MCP-1 mRNA expression induced by carbachol ( P < 0.05 ) ... The activity of NF-kappaB in pancreatic acinar cells was significantly increased ( P < 0.01 ) after treated with M3 cholinergic receptor agonist ( 10 ( -3 ) mol/L carbachol ) in vitro for 30 min. Either M3 cholinergic receptor antagonist ( 10 ( -5 ) mol/L atropine ) or NF-kappaB inhibitor ( 10 ( -2 ) mol/L PDTC ) could obviously inhibit the activation of NF-kappaB and the chemokine MOB-1, MCP-1 mRNA expression induced by carbachol ( P < 0.05 )
Huang et al., Acta Anaesthesiol Scand 2004 : We investigated the time course of enzyme induction and the role of nuclear factor-kappaB (NF-kappaB) in CAT-2 isozymes expression in lipopolysaccharide-(LPS) treated rat lungs
Takada et al., Blood 2004 : Deletion of TNF receptors sensitized the cells to RANKL induced NF-kappaB activation, in order from least to most sensitive of p60 ( -/- ) less than p80 ( -/- ) less than p60 ( -/- ) p80 ( -/- )
Liu et al., J Biomed Sci 2004 (Adenocarcinoma...) : Our results showed that overexpression of RTKN induced robust activation of NF-kappaB , and RTKN mediated NF-kappaB activation was suppressed significantly by C3 transferase, an inhibitor of the small GTPase Rho ... We conclude that Rho/RTKN mediated NF-kappaB activation leading to cell survival may play a key role in gastric tumorigenesis ... We conclude that Rho/RTKN mediated NF-kappaB activation leading to cell survival may play a key role in gastric tumorigenesis
May et al., J Biol Chem 2004 : Deletion of the ULD rendered IKKbeta catalytically inactive and unable to induce NF-kappaB activity, and overexpression of only the ULD dose-dependently inhibited tumor necrosis factor-alpha induced NF-kappaB activity ... Deletion of the ULD rendered IKKbeta catalytically inactive and unable to induce NF-kappaB activity, and overexpression of only the ULD dose-dependently inhibited tumor necrosis factor-alpha induced NF-kappaB activity ... We identified five residues conserved among the larger family of UBL containing proteins and IKKbeta, and alanine scanning revealed that the leucine at position 353 ( Leu ( 353 ) ) is absolutely critical for IKKbeta induced NF-kappaB activation
Hung et al., J Biol Chem 2004 : pp38 MAPK inhibitor SB203580 or eIF2alpha phosphorylation inhibitor 2-aminopurine attenuated the nuclear NF-kappaB DNA binding activity and COX-2 induction
Okajima et al., Curr Vasc Pharmacol 2004 (Hypotension...) : APC inhibits ET-induced TNF-alpha production in vitro in human monocytes by inhibiting activation of NFkappaB and AP-1 by inhibiting degradation of IkappaB and mitogen activated protein kinase pathways, respectively ... APC inhibits ET-induced TNF-alpha production in vitro in human monocytes by inhibiting activation of NFkappaB and AP-1 by inhibiting degradation of IkappaB and mitogen activated protein kinase pathways, respectively
Shishodia et al., J Biol Chem 2004 (Inflammation...) : Guggulsterone suppressed DNA binding of NF-kappaB induced by tumor necrosis factor (TNF), phorbol ester, okadaic acid, cigarette smoke condensate, hydrogen peroxide, and interleukin-1 ... Guggulsterone suppressed DNA binding of NF-kappaB induced by tumor necrosis factor (TNF) , phorbol ester, okadaic acid, cigarette smoke condensate, hydrogen peroxide, and interleukin-1
Abboushi et al., J Immunol 2004 : Ceramide inhibits IL-2 production by preventing protein kinase C-dependent NF-kappaB activation : possible role in protein kinase Ctheta regulation ... As expected, the repression of NF-kappaB activation by ceramide led to the reduction of transcription of the IL-2 gene in a dose dependent manner
Si et al., J Immunol 2004 (Acquired Immunodeficiency Syndrome) : Adenoviral transduction of super-repressor IkappaBalpha, dominant negative MKK6, and dominant negative Ras demonstrated that NF-kappaB and p38 MAPK were involved in LPS induced IFN-inducible protein 10/CXCL10 production ... Interestingly, although LPS induced IL-8/CXCL8 was dependent on NF-kappaB , the baseline or 15d-PGJ ( 2 ) -mediated IL-8/CXCL8 production was NF-kappaB independent ... Interestingly, although LPS induced IL-8/CXCL8 was dependent on NF-kappaB , the baseline or 15d-PGJ ( 2 ) -mediated IL-8/CXCL8 production was NF-kappaB independent
Chang et al., Mol Pharmacol 2004 : In this study, we explore the role of Ras, phosphoinositide-3-OH-kinase (PI3K), Akt, and transcription factor nuclear factor-kappaB (NF-kappaB) in YC-1 induced COX-2 expression in A549 cells ... A Ras inhibitor ( manumycin A ), a PI3K inhibitor ( wortmannin ), an Akt inhibitor ( 1l-6-Hydroxymethyl-chiro-inositol2- [ ( R ) -2-O-methyl-3-O-octadecylcarbonate ] ), and an NF-kappaB inhibitor [ pyrrolidine dithiocarbamate ( PDTC ) ] all reduced YC-1 induced COX-2 expression ... Taken together, these results indicate that YC-1 might activate the sGC/cGMP/PKG pathway to induce Ras and PI3K/Akt activation, which in turn initiates IKKalpha/beta and NF-kappaB activation and finally induces COX-2 expression in A549 cells
Zhang et al., Sheng Li Xue Bao 2004 : These results suggest that hypoxia may lead to IkappaBalpha phosphorylation and NF-kappaB activation through intracellular ROS, and that the regulation of NF-kappaB activity may involve IkappaBalpha phosphorylation and the expressions of each subunit gene of NF-kappaB
Ianaro et al., Naunyn Schmiedebergs Arch Pharmacol 2004 (Endotoxemia) : In vivo treatment with cloricromene ( 2 mg/kg/i.v. ) 30 min before lipopolysaccharide administration reversed the LPS induced loss in tone of the aortic rings, improved their reactivity to phenylephrine, decreased both nitric oxide ( NO ) and TNF-alpha serum levels by inhibiting LPS induced inducible NO synthase and TNF-alpha mRNA expression, and interestingly inhibited LPS induced NF-kappaB activation ... In vivo treatment with cloricromene ( 2 mg/kg/i.v. ) 30 min before lipopolysaccharide administration reversed the LPS induced loss in tone of the aortic rings, improved their reactivity to phenylephrine, decreased both nitric oxide ( NO ) and TNF-alpha serum levels by inhibiting LPS induced inducible NO synthase and TNF-alpha mRNA expression, and interestingly inhibited LPS induced NF-kappaB activation ... In vivo treatment with cloricromene ( 2 mg/kg/i.v. ) 30 min before lipopolysaccharide administration reversed the LPS induced loss in tone of the aortic rings, improved their reactivity to phenylephrine, decreased both nitric oxide ( NO ) and TNF-alpha serum levels by inhibiting LPS induced inducible NO synthase and TNF-alpha mRNA expression, and interestingly inhibited LPS induced NF-kappaB activation ... Our data suggest that cloricromene protects rats from LPS by blocking LPS induced NF-kappaB activation, leading to inhibition of NO and TNF-alpha overproduction and thereby reversing the LPS induced vascular hyporeactivity
Tallant et al., BMC microbiology 2004 (Central Nervous System Neoplasms...) : Surprisingly, a number of cell lines found to be unresponsive to flagellin express TLR5 and expression of exogenous TLR5 in these cells induces NF-kappa B activity in response to flagellin challenge although not robustly
Sée et al., J Cell Biol 2004 (Calcium Signaling...) : Inhibition of the MAPK- but not the PtdIns3K dependent pathway inhibited NF-kappaB signaling, and further, CD1 transcription and cell cycle progression
Kim et al., Cancer Lett 2004 (Inflammation) : Moreover, electrophoretic mobility shift assay experiments indicated that kahweol blocked the LPS induced activation of NF-kappaB
Odabaei et al., Adv Cancer Res 2004 (MAP Kinase Signaling System) : RKIP has been shown to disrupt the Raf-1-MEK1/2 [ mitogen activated protein kinase-ERK ( extracellular signal regulated kinase ) kinase-1/2 ] -ERK1/2 and NF-kappaB signaling pathways, via physical interaction with Raf-1-MEK1/2 and NF-kappaB inducing kinase or transforming growth factor beta activated kinase-1, respectively, thereby abrogating the survival and antiapoptotic properties of these signaling pathways
Kraus et al., Somat Cell Mol Genet 1999 (Liver Neoplasms, Experimental) : Interestingly, LPS mediated activation of NF-kappaB was found to be independent of degradation of IkappaB-alpha or IkappaB-beta
Zhou et al., Zhonghua Wai Ke Za Zhi 2004 (Adenocarcinoma...) : IND may inhibit the activation of NF-kappaB , decrease serum TNF-alpha and IL-6 levels and thus alleviate the cachexia ... IND may inhibit the activation of NF-kappaB , decrease serum TNF-alpha and IL-6 levels and thus alleviate the cachexia
Akira et al., C R Biol 2004 : Upon stimulation, TLR recruits a cytoplasmic adaptor molecule MyD88, then IL-IR associated kinase ( IRAK ), and finally induces activation of NF-kappaB and MAP kinases ... Upon stimulation, TLR recruits a cytoplasmic adaptor molecule MyD88 , then IL-IR associated kinase ( IRAK ), and finally induces activation of NF-kappaB and MAP kinases
Kiningham et al., Biochem J 2004 : Therefore we evaluated IkappaBalpha ( inhibitory kappaBalpha ) and found that co-administration decreased its steady-state level compared with either agent alone, suggesting that enhanced NF-kappaB activation is due to inhibition of IkappaBalpha synthesis
Flemming et al., Cell Immunol 2004 : Disruption of membrane cholesterol stimulates MyD88 dependent NF-kappaB activation in immature B cells
Mihindukulasuriya et al., J Biol Chem 2004 : Protein phosphatase 4 ( PP4 ; also named PPX or PPP4 ) is a PP2A related protein serine/threonine phosphatase with important roles in a variety of cellular processes such as microtubule growth/organization, apoptosis, tumor necrosis factor (TNF)-alpha signaling, and activation of c-Jun N-terminal kinase and NF-kappaB
Mauviel et al., Curr Biol 1993 : We demonstrate that transforming growth factor-beta ( TGF-beta ) does not interfere with cytokine induced IL-8 gene expression, nor does it affect the activity of NF-kappaB-driven promoters
Park et al., Biochem Biophys Res Commun 2004 : A time dependent activation of NF-kappaB induced by 6-OHDA but not MPP ( + ) was also demonstrated by an electrophoretic mobility shift assay ... A competition assay indicated that not only NF-kappaB-p65 but also -p50 is involved in 6-OHDA induced NF-kappaB activity
Pinna et al., Biochem Biophys Res Commun 2004 (Crohn Disease...) : Quantitative-PCR and confocal analysis on a human monocytic cell line indicated that Celastrol acts at the transcriptional level by inhibiting LPS induced NF-kappaB translocation
Singh et al., Biochem Biophys Res Commun 2004 : We also report the involvement of IkappaB-alpha and IkappaB-beta in the persistent activation of NF-kappaB by L. donovani ... We also report the involvement of IkappaB-alpha and IkappaB-beta in the persistent activation of NF-kappaB by L. donovani
Torii et al., Eur J Pharmacol 2004 (Granuloma, Respiratory Tract...) : Vitamin A may inhibit Sephadex induced lung granulomatous formation, and eosinophilic and neutrophilic infiltration due to its suppression of TNF-alpha and eotaxin production, and NF-kappaB activation
Imamura et al., J Biol Chem 2004 (Inflammation) : Fas ligand induces cell-autonomous NF-kappaB activation and interleukin-8 production by a mechanism distinct from that of tumor necrosis factor-alpha ... Reporter gene assays involving wild-type and mutated IL-8 promoters and NF-kappaB- and AP-1 reporter constructs indicated that an FasL induced NF-kappaB and AP-1 activity are required for maximal promoter activity ... FasL induced NF-kappaB activation with slower kinetics than did TNF-alpha, yet this response was cell autonomous and not mediated by secondary paracrine factors ... The death domain of Fas, FADD, and caspase-8 were required for NF-kappaB activation by FasL ... A dominant negative mutant of IKKgamma inhibited the FasL induced NF-kappaB activation ... A dominant negative mutant of IKKgamma inhibited the FasL induced NF-kappaB activation ... However, TRADD and RIP, which are essential for the TNF-alpha induced NF-kappaB activation, were not involved in the FasL induced NF-kappaB activation ... However, TRADD and RIP, which are essential for the TNF-alpha induced NF-kappaB activation, were not involved in the FasL induced NF-kappaB activation ... However, TRADD and RIP, which are essential for the TNF-alpha induced NF-kappaB activation, were not involved in the FasL induced NF-kappaB activation ... Moreover, CLARP/FLIP inhibited the FasL- but not the TNF-alpha induced NF-kappaB activation ... Moreover, CLARP/FLIP inhibited the FasL- but not the TNF-alpha induced NF-kappaB activation ... These results show that FasL induces NF-kappaB activation and IL-8 production by a novel mechanism, distinct from that of TNF-alpha ... In addition, we found that mouse FADD had a dominant negative effect on the FasL induced NF-kappaB activation in HEK293 cells, which may indicate a species difference between human and mouse in the FasL induced NF-kappaB activation
Janssen et al., J Exp Med 2004 (Ectodermal Dysplasia...) : Monocytes did not produce interleukin 12p40 upon stimulation with various TLR stimuli and nuclear translocation of NF-kappaB was impaired
Ichikawa et al., Biol Pharm Bull 2004 : These results suggest that gallate containing catechins, particularly EGCG, inhibits LPS induced IL-12p40 production in murine macrophages by inhibiting p38 MAPK while enhancing p44/p42 ERK, leading to the inhibition of IkappaBalpha degradation and NF-kappaB activation
Isermann et al., Herz 2004 (Atherosclerosis...) : The ligand-RAGE-interaction results in an activation of NF-kappaB , increased expression of cytokines, chemokines, and adhesion molecules and induces oxidative stress
Wu et al., J Neurochem 2004 (Translocation, Genetic) : Consistently, IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of inhibitory kappa B-alpha ( IkappaB-alpha ) was revealed by western blotting and immunofluorescence staining, which was blocked by helenalin, but not by U0126, SB202190, or SP600125 ... Consistently, IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of inhibitory kappa B-alpha ( IkappaB-alpha ) was revealed by western blotting and immunofluorescence staining, which was blocked by helenalin, but not by U0126, SB202190, or SP600125
Wijayanti et al., Antioxid Redox Signal 2004 (MAP Kinase Signaling System) : Role of NF-kappaB and p38 MAP kinase signaling pathways in the lipopolysaccharide dependent activation of heme oxygenase-1 gene expression ... Up-regulation of HO-1 promoter activity by LPS was decreased by pharmacological nuclear factor-kappaB (NF-kappaB) inhibitors and by cotransfected expression vectors with dominant negative isoforms of NF-kappaB inducing kinase, inhibitor of NF-kappaB (IkappaB) kinase beta, and IkappaBalpha ... Up-regulation of HO-1 promoter activity by LPS was decreased by pharmacological nuclear factor-kappaB (NF-kappaB) inhibitors and by cotransfected expression vectors with dominant negative isoforms of NF-kappaB inducing kinase, inhibitor of NF-kappaB (IkappaB) kinase beta , and IkappaBalpha ... Up-regulation of HO-1 promoter activity by LPS was decreased by pharmacological nuclear factor-kappaB (NF-kappaB) inhibitors and by cotransfected expression vectors with dominant negative isoforms of NF-kappaB inducing kinase, inhibitor of NF-kappaB (IkappaB) kinase beta, and IkappaBalpha
Lee et al., Cancer Chemother Pharmacol 2004 : For experimentally induced tumor promotion, we investigated whether the SNL glycoprotein was able to regulate the activity of protein kinase C alpha (PKCalpha) , the DNA binding activation of nuclear factor-kappa B (NF-kappaB) , the activity of NF-kappaB protein, and the production of nitric oxide ( NO ) in HCT-116 cells stimulated with 12-O-tetradecanoylphorbol 13-acetate ( TPA ) using electrophoretic mobility shift assays ( EMSA ), Western blot analysis, and NO assays ... As expected, SNL glycoprotein dose-dependently inhibited PKCalpha translocation, NF-kappaB DNA binding activity, NF-kappaB protein activity and NO production in HCT-116 cells stimulated with TPA ( 61.68 ng/ml, 100 nM )
Wang et al., J Immunol Methods 2004 : This method is based on the mechanism of TLR2 activation whereby extracellular ( EC ) domain mediated heterodimerization of TLR2 and TLR1 activates NF-kappaB and other signalling processes ... Co-expression of IL4-TIR1 with IL4Ralpha-TIR2, but not gammaC-TIR2 , led to constitutive NF-kappaB activation ... Co-expression of IL4-TIR1 with IL4Ralpha-TIR2 , but not gammaC-TIR2, led to constitutive NF-kappaB activation
Bates et al., Exp Cell Res 2004 (Colonic Neoplasms...) : Using a novel model of EMT in colon carcinoma in which the inflammatory cytokine TNF-alpha accelerates this TGF-beta directed process, we report that TNF-alpha stimulation upregulates expression of the chemokine IL-8, and that this upregulation is dependent on the transcription factor NF-kappaB ... Using a novel model of EMT in colon carcinoma in which the inflammatory cytokine TNF-alpha accelerates this TGF-beta directed process, we report that TNF-alpha stimulation upregulates expression of the chemokine IL-8 , and that this upregulation is dependent on the transcription factor NF-kappaB
Teshima et al., Nitric Oxide 2004 : This study investigated whether cycloprodigiosin influenced NF-kappaB activation and induction of iNOS by interleukin-1beta
Lee et al., Biochem Biophys Res Commun 2004 (Inflammation...) : To further investigate the mechanism responsible for the inhibition of iNOS gene expression by butein, we examined the effect of butein on LPS induced nuclear factor-kappaB (NF-kappaB) activation ... The LPS induced DNA binding activity of NF-kappaB was significantly inhibited by butein, and this effect was mediated through inhibition of the degradation of inhibitory factor-kappaB and phosphorylation of Erk1/2 MAP kinase
Tetsuka et al., Eur J Biochem 2004 : In transient transfection assays, RHA enhanced NF-kappaB dependent reporter gene expression induced by p65, tumor necrosis factor-alpha, or NF-kappaB inducing kinase
Van 't Hof et al., J Bone Miner Res 2004 (Bone Resorption) : Mechanistic studies showed that ABD056 caused osteoclast apoptosis and inhibited TNFalpha induced NF-kappaB activation
Kumagai et al., J Biol Chem 2004 (Arteriosclerosis) : To understand the possible signaling mechanism underlying the inducing effect of HNE on COX-2 up-regulation, we examined the phosphorylation events that may lead to COX-2 induction and found that HNE did not stimulate the induction of nitric oxide synthase and activation of NF-kappaB but significantly activated p38 mitogen activated protein kinase and its upstream kinase in RAW264.7 macrophages
Park et al., J Immunol 2004 : Cutting edge : direct interaction of TLR4 with NAD(P)H oxidase 4 isozyme is essential for lipopolysaccharide induced production of reactive oxygen species and activation of NF-kappa B ... In this study, we present the data that LPS induced ROS generation and NF-kappaB activation are mediated by a direct interaction of TLR4 with ( NAD(P)H oxidase 4 ( Nox) 4 ), a protein related to gp91phox ( Nox2 ) of phagocytic cells, in HEK293T cells ... In this study, we present the data that LPS induced ROS generation and NF-kappaB activation are mediated by a direct interaction of TLR4 with ( NAD(P)H oxidase 4 ( Nox) 4 ), a protein related to gp91phox ( Nox2 ) of phagocytic cells, in HEK293T cells ... In this study, we present the data that LPS induced ROS generation and NF-kappaB activation are mediated by a direct interaction of TLR4 with ( NAD(P)H oxidase 4 ( Nox) 4 ), a protein related to gp91phox ( Nox2 ) of phagocytic cells, in HEK293T cells
Laan et al., J Immunol 2004 : In addition, the activity of LPS induced transcription factor AP-1, but not NF-kappaB , was down-regulated by CSE ... In addition, the activity of LPS induced transcription factor AP-1, but not NF-kappaB , was down-regulated by CSE
Lean et al., Biochem Biophys Res Commun 2004 (Bone Resorption) : We found that AP-1, NFkappaB , and NFAT-reporter gene expression was enhanced more greatly by RANKL in RAW cells transfected with the Trx-expression construct
You et al., Biochem Biophys Res Commun 2004 : These findings indicate that PMA induces expression of cPLA(2)alpha at the transcriptional level via an NF-kappaB dependent mechanism
Chang et al., Exp Mol Med 2004 : SNAP also blocked cytokine- induced increase in NF-kappaB activation, iNOS promoter activity, nuclear translocation of cytosolic NF-kappaB p65 subunit, and IkappaBalpha degradation, which correlated with its inhibitory effect on phosphorylation and ubiquitination of IkappaB
Romics et al., Hepatology 2004 (Fatty Liver...) : In lean mice, in contrast, acute alcohol attenuated LPS induced TNF-alpha, IL-6 production, and NF-kappaB activation through reduced IkappaB-alpha degradation and induction of p50/p50 homodimers
Oster et al., Blood 1992 : However, the action of IL-3 on synthesis of IL-1 beta mRNA differs from that of other CSFs : While GM-CSF and M-CSF induce binding activity of the nuclear factor (NF) kappa B , IL-3 treatment of MNP has no profound effect on NF kappa B binding to DNA ... However, the action of IL-3 on synthesis of IL-1 beta mRNA differs from that of other CSFs : While GM-CSF and M-CSF induce binding activity of the nuclear factor (NF) kappa B , IL-3 treatment of MNP has no profound effect on NF kappa B binding to DNA
Khoshnan et al., J Neurosci 2004 (Nerve Degeneration) : In inducible PC12 cells and in HD transgenic mice, mutant Htt activates the IkappaB kinase complex (IKK) , a key regulator of NF-kappaB
Riis et al., Arch Dermatol Res 2004 : In unstimulated keratinocytes no NF-kappaB binding to the IL-8 kappaB binding sequence was detectable, whereas stimulation with IL-1alpha ( 10 ng/ml ) led to a significant ( P < 0.05 ) induction of NF-kappaB binding
Tsukihara et al., Am J Reprod Immunol 2004 : IL-1beta induced expression of phosphorylated IkappaB and the activation of NF-kappaB
Seo et al., Cancer Res 2004 (Adenocarcinoma...) : Taken together, these results suggest that estrogen induces the production of angiogenic factors via a mechanism involving PAF mediated NF-kappaB activation
Loercher et al., Cancer Res 2004 (Carcinoma, Squamous Cell...) : The ACTACAG motif implicated in the NF-kappaB dependent down-regulation of mRNA expression of MyoD and Sox9 was detected in the coding portion of about 15 % of genes showing increased or decreased expression
Jung et al., Int J Oncol 2004 (MAP Kinase Signaling System) : The cIAP1, cIAP2 and XIAP could enhance the NF-kappaB transcription and the chimeric NF-kappaB-p65 transactivation ... The cIAP1, cIAP2 and XIAP could enhance the NF-kappaB transcription and the chimeric NF-kappaB-p65 transactivation ... Taken together, LPS dependent NF-kappaB transactivation may be involved in extracellular signal regulated kinase ( ERK ) pathway and IAPs
Kawakubo et al., Oncogene 2004 (Breast Neoplasms) : BTG2 , a p53-inducible antiproliferative gene, is stimulated in breast cancer cells by activation of nuclear factor kappa B (NF-kappaB)
Kuphal et al., Oncogene 2004 (Melanoma) : Loss of E-cadherin leads to upregulation of NFkappaB activity in malignant melanoma ... Here, we show that loss of E-cadherin leads to induction of nuclear factor kappa B (NFkappaB) activity in melanoma cell lines ... Furthermore, cytoplasmatic beta-catenin induced p38 and NFkappaB activation in malignant melanoma ... In summary, we conclude that loss of E-cadherin and cytoplasmatic beta-catenin induces p38 mediated NFkappaB activation, potentially revealing an important mechanism of tumorigenesis in malignant melanomas ... In summary, we conclude that loss of E-cadherin and cytoplasmatic beta-catenin induces p38 mediated NFkappaB activation, potentially revealing an important mechanism of tumorigenesis in malignant melanomas ... In summary, we conclude that loss of E-cadherin and cytoplasmatic beta-catenin induces p38 mediated NFkappaB activation, potentially revealing an important mechanism of tumorigenesis in malignant melanomas
Tantini et al., Amino Acids 2004 (MAP Kinase Signaling System) : Moreover, pharmacological NF-kappaB inhibition reduced the increase in caspase activity and cell death elicited by etoposide, suggesting that NF-kappaB is involved in signaling to apoptosis
Pålsson-McDermott et al., Immunology 2004 : A later response to LPS makes use of TIR-domain containing adapter inducing interferon-beta ( TRIF ) and TRIF related adapter molecule (TRAM), and leads to the late activation of NF-kappaB and IRF3, and to the induction of cytokines, chemokines, and other transcription factors
Jüttler et al., Neuropharmacology 2004 : This suggests that neither the antioxidant properties of cannabinoids nor binding to CB1 or CB2 receptors are responsible for the inhibition of NF-kappaB activity and apoptosis
Ohta et al., Biochem Biophys Res Commun 2004 : When overexpressed with MD-2, MD-2B competitively suppressed NF-kappaB activity induced by LPS
Lamkanfi et al., J Biol Chem 2004 : Unlike COP/Pseudo-ICE and procaspase-1, INCA does not interact with RIP2 and does not induce NF-kappaB activation
Aggarwal et al., J Immunol 2004 (Melanoma) : In the present report we investigated the effect of MDA-7 on NF-kappaB activation and on TNF induced NF-kappaB activation and apoptosis in human embryonic kidney 293 cells ... In the present report we investigated the effect of MDA-7 on NF-kappaB activation and on TNF induced NF-kappaB activation and apoptosis in human embryonic kidney 293 cells ... However, TNF induced NF-kappaB activation was significantly enhanced in mda-7 transfected cells, as indicated by DNA binding, p65 translocation, and NF-kappaB dependent reporter gene expression ... Mda-7 transfection also potentiated NF-kappaB reporter activation induced by TNF receptor associated death domain and TNF receptor associated factor-2 ... Mda-7 transfection also potentiated NF-kappaB reporter activation induced by TNF receptor associated death domain and TNF receptor associated factor-2 ... Cytoplasmic MDA-7 with deleted signal sequence was as effective as full-length MDA-7 in potentiating TNF induced NF-kappaB reporter activity ... Secretion of MDA-7 was not required for the potentiation of TNF induced NF-kappaB activation ... Overall, our results indicate that stable or transient MDA-7 expression alone does not substantially activate NF-kappaB , but potentiates TNF induced NF-kappaB activation and NF-kappaB regulated gene expression ... Overall, our results indicate that stable or transient MDA-7 expression alone does not substantially activate NF-kappaB, but potentiates TNF induced NF-kappaB activation and NF-kappaB regulated gene expression
Kim et al., J Immunol 2004 (MAP Kinase Signaling System...) : In contrast, p75 is nonredundantly coupled to sustained AKT activity and NF-kappaB activation in response to TCR/CD28 mediated stimulation
Mason et al., Infect Immun 2004 : Recent studies suggest that components of the TLR pathway are required for the production of IL-12 in response to the parasite Toxoplasma gondii ; however, the production of IL-12 in response to this parasite is independent of NF-kappaB activation
Wehkamp et al., Infect Immun 2004 : NF-kappaB- and AP-1 mediated induction of human beta defensin-2 in intestinal epithelial cells by Escherichia coli Nissle 1917 : a novel effect of a probiotic bacterium ... Luciferase gene reporter analyses and site directed mutagenesis experiments demonstrated that functional binding sites for NF-kappaB and AP-1 in the hBD-2 promoter are required for induction of hBD-2 through E. coli Nissle 1917
Wu et al., Infect Immun 2004 : Our data suggest that BFT stimulated IL-8 secretion involves tyrosine kinase dependent activation of nuclear factor-kappaB (NF-kappaB) as well as activation of the mitogen activated protein kinases ( MAPKs ), p38 and extracellular signal related kinase ... Simultaneous activation of NF-kappaB and MAPKs appears necessary for secretion of IL-8 by HT29/C1 cells treated with BFT
Potrovita et al., J Neurosci 2004 (Brain Ischemia...) : Transfection of a nuclear factor (NF)-kappaB-luciferase fusion gene demonstrated that TWEAK stimulated transcriptional activity of NF-kappaB through Fn14 and the IkappaB kinase
Shin et al., Planta Med 2004 : Furthermore, 23-hydroxyursolic acid ( 2 ) inhibited the LPS induced DNA binding activity of nuclear factor- kappaB (NF- kappaB) , which was associated with a decrease of p65 protein levels in the nucleus
Equils et al., Antimicrob Agents Chemother 2004 : Human immunodeficiency virus type 1 protease inhibitors block toll-like receptor 2 (TLR2)- and TLR4 Induced NF-kappaB activation ... Here, we assessed whether HIV type 1 protease inhibitors (PI) block microbial antigen activation of NF-kappaB ... We observed that PI pretreatment blocked the TLR2- and TLR4- as well as the TNF-alpha mediated NF-kappaB activation, in a dose dependent manner
Liao et al., Mol Carcinog 2004 : Western blot analyzes and gel-shift assays revealed that garcinol strongly blocks the activation of eukaryotic transcription factor nuclear factor-kappa B (NF-kappa B)-induced by LPS ... Based on these data, we demonstrated that inhibition of LPS induced NF-kappa B activation occurred through suppressing the phosphorylation of I kappa B alpha and p38 mitogen activated kinase ( MAPK )
Zhai et al., Glia 2004 : Reporter gene assays demonstrated KC-upregulated NF-kappa B transcriptional activity, and this effect was inhibited by dominant negative IkappaBalpha
Guo et al., Glia 2004 (Calcium Signaling...) : Caffeic acid phenethyl ester ( CAPE ), a specific inhibitor of NF-kappaB activation, not only abrogated IL-1beta induced NF-kappaB promoter activation , but also blocked IL-1beta mediated induction of NK-1R gene expression
Iwasaki et al., FEBS Lett 1992 : Herbimycin A blocks IL-1 induced NF-kappa B DNA binding activity in lymphoid cell lines ... The mechanism by which interleukin-1 alpha (IL-1 alpha) activates NF-kappa B DNA binding activity is not completely understood ... While it is well established that protein kinase C can activate NF-kappa B, neither protein kinase C nor protein kinase A appears to be critical in the induction of NF-kappa B by IL-1 alpha ... Since a number of growth factors signal via protein tyrosine kinase, in this study we examined a possible involvement of protein tyrosine kinase in the IL-1 alpha induced NF-kappa B ... Since a number of growth factors signal via protein tyrosine kinase, in this study we examined a possible involvement of protein tyrosine kinase in the IL-1 alpha induced NF-kappa B ... The results showed that in the murine pre-B cell line 70Z/3 and in the murine T cell line EL-4 6.1 C10 IL-1 alpha induced NF-kappa B was associated with transient increase in protein tyrosine kinase activity ... Pre-treatment of these cell lines with herbimycin A, an inhibitor of tyrosine kinase activity, blocked the IL-1 alpha enhanced protein tyrosine kinase activity and the IL-1 alpha induced NF-kappa B DNA binding activity ... Herbimycin A at concentrations sufficient to block IL-1 alpha induced NF-kappa B did not block the phorbol 12-myristate 13-acetate ( PMA ) -induced NF-kappa B ... The data suggest that IL-1 alpha and PMA activate NF-kappa B by different pathways and that induction of NF-kappa B DNA binding activity by IL-1 might be dependent on protein tyrosine phosphorylation
Marx et al., Diabetes 2004 : These effects on RAGE expression were caused by an inhibition of nuclear factor-kappaB (NF-kappaB) activation at the proximal NF-kappaB site of the RAGE promoter
Lappas et al., Placenta 2004 : Therefore, the aim of this study was to determine whether NF-kappaB and PPAR-gamma also regulate PTHrP release from human fetal membranes ... This study demonstrates that PTHrP release from human fetal membranes is regulated by inhibitors of NF-kappaB , and ligands of PPAR-gamma
Kim et al., Biochem Pharmacol 2004 : Epicatechin significantly reduced IL-1beta induced nitrite production, iNOS protein and mRNA expressions, and it also inhibited IL-1beta induced IkappaBalpha protein degradation, NF-kappaB activation , and iNOS promoter activity ... These results suggest that epicatechin inhibits the IL-1beta induced iNOS expression by down regulating NF-kappaB activation, and protecting beta-cells from IL-1beta
Prasad et al., Free Radic Biol Med 2004 : Plasma zinc, MDA, HAE, and 8-OHdG levels ; LPS induced TNF-alpha and IL-1beta mRNA ; and ex vivo TNF-alpha induced NF-kappaB activity in mononuclear cells ( MNC ) were determined before and after supplementation ... Ex vivo, zinc protected MNC from TNF-alpha induced NF-kappaB activation
Demarco et al., Free radical research 2004 : LA and hyperthermia attenuated LPS induced increases in nuclear NF-kappaB , iNOS protein, and media nitrite concentrations
Afaq et al., Int J Cancer 2005 (Edema...) : We also found that topical application of PFE resulted in inhibition of TPA induced phosphorylation of ERK1/2, p38 and JNK1/2, as well as activation of NF-kappaB and IKKalpha and phosphorylation and degradation of IkappaBalpha ... We also found that topical application of PFE resulted in inhibition of TPA induced phosphorylation of ERK1/2 , p38 and JNK1/2, as well as activation of NF-kappaB and IKKalpha and phosphorylation and degradation of IkappaBalpha
Moodie et al., FASEB J 2004 : However, H2O2, tumor necrosis factor-alpha (TNF-alpha), and IL-1beta significantly increased NF-kappaB activation and expression of IL-8 compared with control cells
Fan et al., Endocrinology 2005 : Interestingly, activation of NF-kappaB by either forced expression of the p65 subunit or NF-kappaB inducing kinase up-regulated ArPII activity ... Positive regulation of aromatase by endogenous NF-kappaB was also suggested by the fact that NF-kappaB-specific inhibitors suppress basal activity of the aromatase gene ... Altogether, these results indicate that activation of a nuclear receptor system, constituted by PPARgamma and RXR, down-regulates aromatase expression through the suppression of NF-kappaB dependent aromatase activation and thus provide a new insight in the mechanism of regulation of the aromatase gene
Wachter et al., J Biol Chem 2004 (Inflammation) : Surprisingly, cFLIP ( L ) specifically blocked TRAIL induced NF-kappaB activation and TRAIL dependent induction of the proinflammatory target gene interleukin-8 ... Surprisingly, cFLIP ( L ) specifically blocked TRAIL induced NF-kappaB activation and TRAIL dependent induction of the proinflammatory target gene interleukin-8 ... Taken together, our data demonstrate that cFLIP ( L ) is not only a central antiapoptotic modulator of TRAIL mediated apoptosis but also an inhibitor of TRAIL induced NF-kappaB activation and subsequent proinflammatory target gene expression
Lavagno et al., Eur J Pharmacol 2004 (Arthritis, Rheumatoid) : Anti-inflammatory drugs and tumor necrosis factor-alpha production from monocytes : role of transcription factor NF-kappa B and implication for rheumatoid arthritis therapy
Morrison et al., Virology 2004 : In spite of this BZLF1 associated increase in the nuclear form of NF-kappaB, BZLF1 did not induce binding of NF-kappaB to NF-kappaB responsive promoters ( as determined by chromatin immunoprecipitation assay ) in vivo, although TNF-alpha treatment induced NF-kappaB binding as expected
Lee et al., J Neuroimmunol 2004 : The modulation of LPS induced NF-kappaB by ethanol was due to the inhibition of coactivator p300
Heo et al., Toxicol In Vitro 2004 (Breast Neoplasms) : To test the effect of SNL glycoprotein on the DNA binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1), and nitric oxide ( NO ) production, these experiments were carried out using electrophoretic mobility shift assays ( EMSA ), western blot analysis, and the Griess method ... Results in this experiment showed that SNL glycoprotein inhibits 12-O-Tetra decanoylphorbol-13-acetate ( TPA ; 100 nM ) -induced DNA binding activities of NF-kappaB and AP-1, and enhances NO production in MCF-7 cells
Li et al., J Biol Chem 2004 : BRE, brain and reproductive organ expressed protein, was found previously to bind the intracellular juxtamembrane domain of a ubiquitous death receptor, tumor necrosis factor receptor 1 ( TNF-R1 ), and to down-regulate TNF-alpha induced activation of NF-kappaB
Bannerman et al., Am J Pathol 2004 : Conversely, mouse embryo fibroblasts (MEF) obtained from FLIP -/- mice showed enhanced LPS induced NF-kappaB activation relative to those obtained from wild-type mice
Xiao et al., Cancer Biol Ther 2004 (Multiple Myeloma) : NF-kappaB activates IL-6 expression through cooperation with c-Jun and IL6-AP1 site, but is independent of its IL6-NFkappaB regulatory site in autocrine human multiple myeloma cells ... The mechanism of NF-kappaB activation of IL-6 appears to occur through the cooperation with c-Jun protein, that constitutively occupies the IL6-AP1 site, and this indicates a novel transcriptional mechanism for NF-kappaB in the activation of NF-kappaB-driven genes
Legembre et al., Cell cycle (Georgetown, Tex.) 2004 (Neoplasms) : In fact overexpression of apoptosis inhibitors such as Bcl-2 or c-FLIPL in these cells results in decreased activation of NF-kappaB through CD95
Guo et al., Thromb Haemost 2004 (Inflammation) : From our findings, we conclude that fibrinogen regulates NF-kappaB activation and expression of inflammatory chemokines in endothelial cells and may be involved in mediating inflammatory processes
Ozawa et al., J Immunol 2004 (Periodontitis) : Furthermore, excess amounts of IL-2 restored the inhibitory effect of anti-IL-15 , inhibition of NF-kappaB abrogated the expression of IL-2Rbeta and gammac, and IL-2-induced-nuclear translocation of NF-kappaB was completely inhibited by the RNA interference in HGF ... Furthermore, excess amounts of IL-2 restored the inhibitory effect of anti-IL-15, inhibition of NF-kappaB abrogated the expression of IL-2Rbeta and gammac, and IL-2-induced-nuclear translocation of NF-kappaB was completely inhibited by the RNA interference in HGF
Lee et al., J Biol Chem 2004 (Inflammation) : We found that TGase 2 activates the transcriptional activator nuclear factor (NF)-kappaB and thereby enhances LPS induced expression of inducible nitric-oxide synthase ... TGase 2 activates NF-kappaB via a novel pathway ... The findings are consistent with a model in which LPS induced NF-kappaB activation is the result of phosphorylation of I-kappaBalpha by I-kappaB kinase as well as I-kappaBalpha polymerization by TGase 2 ... The findings are consistent with a model in which LPS induced NF-kappaB activation is the result of phosphorylation of I-kappaBalpha by I-kappaB kinase as well as I-kappaBalpha polymerization by TGase 2
Wang et al., FEBS Lett 2004 : We show here that A20, a NF-kappaB-inducible zinc finger protein that has been demonstrated to be an inhibitor of TNF induced NF-kappaB activation and a physiological suppressor of inflammatory response, potently inhibited TLR3- and Sendai virus mediated activation of ISRE and NF-kappaB and IFN-beta promoter in reporter gene assays ... A20 also inhibited TRIF-, but not its downstream signaling components TBK1- , IKKbeta-, and IKKepsilon mediated activation of ISRE and NF-kappaB and IFN-beta promoter ... A20 also inhibited TRIF-, but not its downstream signaling components TBK1-, IKKbeta-, and IKKepsilon mediated activation of ISRE and NF-kappaB and IFN-beta promoter
Yamauchi et al., Fertil Steril 2004 (Endometriosis) : To examine the involvement of nuclear factor-kappaB (NF-kappaB) and mitogen activated protein kinase ( MAPK ) in the induction of interleukin-6 (IL-6) by tumor necrosis factor-alpha (TNF-alpha) in endometriotic stromal cells ( ESC ) ... The NF-kappaB inhibitor ( TPCK ) and MAPK inhibitor ( U0126 ) blocked the TNF-alpha induced IL-6 expression ... These findings demonstrate that NF-kappaB and AP-1 activation is critical for TNF-alpha induced IL-6 expression in endometriotic stromal cells
Iba et al., Fertil Steril 2004 (Endometriosis) : We determined the effect of LPS on the production of TNFalpha and IL-8 and the effect of IL-8 antisense oligonucleotide and nuclear factor-kappaB (NF-kappaB) inhibitor on IL-8 production using ELISA
Seabra et al., Biochem Biophys Res Commun 2004 (Inflammation) : TGF-beta1 led to inducible nitric oxide synthase (iNOS) degradation, actin filament ( F-actin ) depolymerization, and lack of nuclear factor-kappaB (NF-kappaB) in the nucleus
Prince et al., Am J Physiol Lung Cell Mol Physiol 2004 (Chorioamnionitis...) : Similar to the signaling pathways in inflammatory cells, LPS activated NF-kappaB in fetal lung explants
Dijkstra et al., J Pathol 2004 (Colonic Neoplasms...) : Oxidative stress prevented iNOS induction in an NF-kappaB dependent manner but increased HO-1 expression in CM-exposed DLD-1 cells ... Oxidative stress prevented iNOS induction in an NF-kappaB dependent manner but increased HO-1 expression in CM-exposed DLD-1 cells
Li et al., Cancer 2004 (Pancreatic Neoplasms) : NF-kappaB and IkappaB kinase (IKK) were constitutively active in all human pancreatic carcinoma cell lines examined, and curcumin consistently suppressed NF-kappaB binding ( as assessed using an electrophoretic mobility gel-shift assay ) and IKK activity
Zhang et al., Zhejiang Da Xue Xue Bao Yi Xue Ban 2004 : As2O3 could induce apoptosis of K562 cells, associated with the degradation of IkappaB-alpha and the activation of NF-kappaB
Yip et al., J Bone Miner Res 2004 (Bone Resorption...) : Furthermore, PAR inhibited LPS induced NF-kappaB activation , p65 translocation, and IkappaB-alpha degradation both in mature osteoclasts and their precursors in a time- and dose dependent manner
Taddeo et al., J Virol 2004 : Earlier we reported that NF-kappaB is activated by protein kinase R (PKR) in herpes simplex virus 1-infected cells
Pajonk et al., Oncogene 2004 (Neoplasms) : Using electrophoretic mobility shift assays, we could demonstrate strong activation of NF-kappaB by erythropoietin-receptor signaling in HeLa cells ... Activation of NF-kappaB did not require degradation of IkappaBalpha and was not prevented by proteasome inhibition
Liu et al., Oncogene 2004 : Rho/Rhotekin mediated NF-kappaB activation confers resistance to apoptosis ... Rho/Rhotekin mediated NF-kappaB activation confers resistance to apoptosis ... Reporter gene assays and electrophoretic mobility shift assay confirmed that RTKN overexpression led to constitutive activation of NF-kappaB through the phosphorylation of IkappaB by IKKbeta
Tsukinoki et al., Clin Exp Nephrol 2004 (Disease Progression...) : IL1beta significantly, and LPS or gammaIFN weakly activated NFkappaB , but none of these agents activated NFkappaB/Rel related nuclear factor of activated T cells ( NFATc ) or IFN regulatory factor-1 ... Lactacystin mediated inhibition of NFkappaB reduced FasL protein levels
Pazdrak et al., Gastroenterology 2004 : TNFalpha suppresses human colonic circular smooth muscle cell contractility by SP1- and NF-kappaB mediated induction of ICAM-1
Jennings et al., Am Surg 2004 : Nuclear factor-kappaB (NF-kappaB) regulates the expression of TNF ... Because NF-kappaB is activated by both LPS and ROS, we hypothesized that an inhibitor of NF-kappaB , pyrrolidine dithiocarbamate ( PDTC ), would block release of TNF from the heart stimulated by these two agents ... Because NF-kappaB is activated by both LPS and ROS, we hypothesized that an inhibitor of NF-kappaB, pyrrolidine dithiocarbamate ( PDTC ), would block release of TNF from the heart stimulated by these two agents
Qian et al., Immunity 2004 (Hypergammaglobulinemia) : CD40- and BAFF mediated survival is significantly increased in Act1-deficent B cells, with stronger IkappaB phosphorylation, processing of NF-kappaB2 ( p100/p52 ), and activation of JNK, ERK , and p38 pathways, indicating that Act1 negatively regulates CD40- and BAFF mediated signaling events
Chen et al., Biochem Biophys Res Commun 2004 : Nontypeable Haemophilus influenzae lipoprotein P6 induces MUC5AC mucin transcription via TLR2-TAK1 dependent p38 MAPK-AP1 and IKKbeta-IkappaBalpha-NF-kappaB signaling pathways ... Nontypeable Haemophilus influenzae lipoprotein P6 induces MUC5AC mucin transcription via TLR2-TAK1 dependent p38 MAPK-AP1 and IKKbeta-IkappaBalpha-NF-kappaB signaling pathways ... Moreover, P6 induces MUC5AC transcription via TLR2-MyD88-IRAK1-TRAF6-TAK1 dependent p38 MAPK-AP1 and IKKbeta-IkappaBalpha-NF-kappaB signaling pathways ... Moreover, P6 induces MUC5AC transcription via TLR2-MyD88-IRAK1-TRAF6-TAK1 dependent p38 MAPK-AP1 and IKKbeta-IkappaBalpha-NF-kappaB signaling pathways ... Moreover, P6 induces MUC5AC transcription via TLR2-MyD88-IRAK1-TRAF6-TAK1 dependent p38 MAPK-AP1 and IKKbeta-IkappaBalpha-NF-kappaB signaling pathways ... Moreover, P6 induces MUC5AC transcription via TLR2-MyD88-IRAK1-TRAF6-TAK1 dependent p38 MAPK-AP1 and IKKbeta-IkappaBalpha-NF-kappaB signaling pathways ... Moreover, P6 induces MUC5AC transcription via TLR2-MyD88-IRAK1-TRAF6-TAK1 dependent p38 MAPK-AP1 and IKKbeta-IkappaBalpha-NF-kappaB signaling pathways
Kwon et al., Mol Cell Biol 2004 : Tumor necrosis factor alpha induction of NF-kappaB requires the novel coactivator SIMPL ... Signaling molecule that interacts with mouse pelle-like kinase ( SIMPL ) is a signaling component required for tumor necrosis factor alpha (TNF-alpha) dependent but not interleukin-1 dependent NF-kappaB activation ... These data support a model in which TNF-alpha activation of NF-kappaB dependent-gene expression requires nuclear relocalization of p65 as well as nuclear relocalization of SIMPL, generating a TNF-alpha-specific induction of gene expression
Chang et al., J Cell Biochem 2004 : Although LIGHT could not affect IFN-gamma induced STAT1 phosphorylation and transactivation activity, which was required for the sensitization of cell death, survival NF-kappaB signaling of LIGHT was inhibited by IFN-gamma
Wang et al., Am J Physiol Lung Cell Mol Physiol 2005 : IL-1beta induced VCAM-1 expression was significantly blocked by the specific NF-kappaB inhibitors helenalin and pyrrolidine dithiocarbamate ... As expected, IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha were blocked by helenalin but not by U0126, SB-202190, or SP-600125 ... As expected, IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha were blocked by helenalin but not by U0126, SB-202190, or SP-600125
Hanson et al., Cancer Res 2004 (Cell Transformation, Neoplastic) : Supportive of the importance of NF-kappaB in transformation, Ras induced cellular transformation can be suppressed by expression of IkappaBalpha , an inhibitor of NF-kappaB , or by dominant negative forms of the upstream activator IkappaB kinase (IKK)
Murata-Ohsawa et al., Int J Mol Med 2004 : The TNF-alpha induced activation of c-Jun N-terminal kinase (JNK), p38, and NF-kappaB was not affected by Delta-1 stimulation ... The TNF-alpha induced activation of c-Jun N-terminal kinase (JNK), p38, and NF-kappaB was not affected by Delta-1 stimulation
Hoberg et al., Mol Cell 2004 : Here, we demonstrate that NF-kappaB transcription requires IKKalpha to phosphorylate SMRT on chromatin, stimulating the exchange of corepressor for coactivator complexes ... Here, we demonstrate that NF-kappaB transcription requires IKKalpha to phosphorylate SMRT on chromatin, stimulating the exchange of corepressor for coactivator complexes
Molinero et al., J Immunol 2004 : Hence, we conclude that NF-kappaB regulates MICA expression on activated T lymphocytes and HeLa tumor cells, by binding to a specific sequence in the long intron 1 of the MICA gene
Jia et al., Zhonghua Wai Ke Za Zhi 2004 : Low NF-kappaB activity was only detected at day 5 and day 7 in Wistar-to-Wistar group after transplantation, meanwhile low IFN-gamma mRNA expression was detected at any time in this group ... CsA down-regulates NF-kappaB activity and further inhibit IFN-gamma gene transcription
Jang et al., Biochem Pharmacol 2004 : Catalase induces the expression of inducible nitric oxide synthase through activation of NF-kappaB and PI3K signaling pathway in Raw 264.7 cells ... Catalase induces the expression of inducible nitric oxide synthase through activation of NF-kappaB and PI3K signaling pathway in Raw 264.7 cells
Stewart et al., Proc Natl Acad Sci U S A 2004 (Nasopharyngeal Neoplasms) : The LMP2A mediated suppression of IL-6 was a consequence of NF-kappaB inhibition
Guseva et al., Mol Cancer Res 2004 (Prostatic Neoplasms) : TRAIL effects on mitochondrial NF-kappaB-DNA binding and mitochondrial genome encoded mRNA levels also depend on Bcl-2 overexpression
Nelson et al., Science 2004 : NF-kappaB regulation of IkappaBalpha transcription represents a delayed negative feedback loop that drives oscillations in NF-kappaB translocation
Gopee et al., Cell Biol Toxicol 2004 : The aim of current study was to investigate the effect of PKCalpha activation by FB1 on NF-kappaB activation and subsequently on TNFalpha gene expression and caspase 3 induction in LLC-PK1 cells ... In summary, we hereby demonstrate that the FB1 activation of NF-kappaB and sequential induction of TNFalpha expression resulting in the subsequent increase in caspase 3 activity are all dependent on PKCalpha stimulation in LLC-PK1 cells
Hajishengallis et al., Infect Immun 2004 (Inflammation) : In this regard, the ability of LT-IIbB to activate NF-kappaB and induce TNF-alpha and IL-8 was antagonized by the LT-IIb holotoxin ... In this regard, the ability of LT-IIbB to activate NF-kappaB and induce TNF-alpha and IL-8 was antagonized by the LT-IIb holotoxin
Starenki et al., Clin Cancer Res 2004 (Carcinoma...) : Treatment with DHMEQ substantially inhibited the translocation of p65 and p50 NF-kappaB subunits to the nucleus, the DNA binding activity of the RelA/p65, NF-kappaB dependent expression of the inhibitor of apoptosis (IAP)-family proteins, cIAP-1, cIAP-2, and XIAP, and the de novo synthesis of inhibitor of nuclear factor kappaB alpha
Reddy et al., Planta Med 2004 : In the present study, an extract from stem bark of Cinnamomum cassia Blume ( Lauraceae ) was discovered to have an inhibitory effect on LPS induced NF-KB transcriptional activity, which was determined using macrophages RAW 264.7 transfected stably with an alkaline phosphatase reporter construct containing four copies of the NF-KB binding KB sequence ... Both trans-cinnamaldehyde and 2-methoxycinnamaldehyde inhibited LPS induced DNA binding activity of NF-KB in addition to NF-KB transcription-al activity
Cacicedo et al., Biochem Biophys Res Commun 2004 : AMPK inhibits fatty acid induced increases in NF-kappaB transactivation in cultured human umbilical vein endothelial cells ... The increases in NF-kappaB activation and VCAM-1 expression caused by palmitate were markedly inhibited by co-incubation with the AMPK activator AICAR and, where studied, by expression of a constitutively active AMPK ... Likewise, AMPK activation inhibited the increase in NF-kappaB reporter gene expression observed in HUVEC incubated with TNF-alpha ... The results suggest that AMPK inhibits the activation of NF-kappaB caused by both palmitate and TNF-alpha
Ho et al., Br J Pharmacol 2004 : We showed that Tet inhibited NF-kappaB DNA binding activities induced by various stimuli, including CD28 costimulation ... Since Tet itself did not affect NF-kappaB binding to its corresponding DNA sequence, the results suggested that Tet might regulate NF-kappaB upstream signaling molecules
Gong et al., J Am Soc Nephrol 2004 (Disease Progression...) : HGF also blunted TNF-alpha induced nuclear translocation and activation of NF-kappaB , a pivotal transcription factor that regulates chemokine expression
Zaidi et al., Invest Ophthalmol Vis Sci 2004 (Anoxia...) : Changes in binding and internalization of P. aeruginosa were measured using cellular association and gentamicin-exclusion assays, and expression of CFTR and activation of NF-kappaB in response to hypoxia were determined by confocal laser microscopy and quantitative measurements of NF-kappaB activation ... Hypoxia increased CFTR expression and NF-kappaB nuclear translocation in rabbit and human cells with wild-type CFTR
Tokuda et al., J Endod 2004 : We also found that SP enhanced LPS induced stimulation of NF-kappaB binding activity
Adams et al., J Nutr 2004 : Additionally, the use of a p-21 activated kinase ( PAK1 ) inhibitory construct ( PAK ( R299 ) ) demonstrated that PAK1 was also required for NFkappaB transcriptional activation by 1,25 ( OH ) ( 2 ) D ( 3 )
Legembre et al., EMBO Rep 2004 : Induction of apoptosis and activation of NF-kappaB by CD95 require different signalling thresholds
Ozeki et al., Anticancer Res 2004 (Breast Neoplasms) : In addition, over expression of the human SOD2 gene ( MnSOD ) inhibited NF-kappaB and pGL3CB1EI1 activity, indicating that superoxide or some species derived from superoxide may have participated in the up-regulation of reporter activity in response to chronic exposure to fractionated ionizing radiation
Cheng et al., Virology 2004 : Furthermore, TNF induced activation of NF-kappaB occurred earlier than WNV induced NF-kappaB activation
Sole et al., J Cell Biol 2004 (MAP Kinase Signaling System) : FAIM overexpression promoted NF-kappa B activation, and blocking this activation by using a super-repressor I kappa B alpha or by carrying out experiments using cortical neurons from mice that lack the p65 NF-kappa B subunit prevented FAIM induced neurite outgrowth
Mühlbauer et al., Biochem Biophys Res Commun 2004 (Fibrosis...) : LPS mediated NFkappaB activation varies between activated human hepatic stellate cells from different donors ... Here, we investigated the LPS induced NFkappaB activation in activated HSC from different human donors ... LPS induced NFkappaB activity and IL-8 expression revealed a significant correlation but differed significantly comparing HSC from individual donors
Kim et al., Biochem Biophys Res Commun 2004 : KL-1156 compound exhibited a dose dependent inhibitory effect on LPS induced NF-kappaB transcriptional activity in macrophages RAW 264.7 ... Furthermore, the compound inhibited LPS induced nuclear translocation of NF-kappaB p65 and DNA binding activity of NF-kappaB complex, in parallel, but did not affect IkappaBalpha degradation
Yamazaki et al., J Biol Chem 2005 : We have recently identified an inducible nuclear factor-kappaB (NF-kappaB) regulator, IkappaB-zeta, which is induced by microbial ligands for Toll-like receptors such as lipopolysaccharide and the proinflammatory cytokine interleukin (IL)-1beta but not by tumor necrosis factor (TNF)-alpha ... Therefore, IkappaB-zeta induction requires both NF-kappaB activation and stimulus-specific stabilization of its mRNA
Wadgaonkar et al., J Cell Biochem 2005 : Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha induced NFkappaB activity ... Endothelial cell myosin light chain kinase (MLCK) regulates TNFalpha induced NFkappaB activity ... Both TNFalpha induced increase in NFkappaB dependent transactivation measured by NFkappaB luciferase reporter assay ( approximately fivefold ) and nuclear translocation of NFkappaB were significantly inhibited by MLCK-selective inhibitors, KT5926 ( 60 % inhibition of luciferase activity ) and ML7 ( 50 % decrease ) ... Both TNFalpha induced increase in NFkappaB dependent transactivation measured by NFkappaB luciferase reporter assay ( approximately fivefold ) and nuclear translocation of NFkappaB were significantly inhibited by MLCK-selective inhibitors, KT5926 ( 60 % inhibition of luciferase activity ) and ML7 ( 50 % decrease ) ... Furthermore, our data revealed that inhibition of MLCK attenuated the TNFalpha induced IkappaB phosphorylation, translocation of p65, NFkappaB-DNA binding, and NFkappaB transcriptional activity ... Furthermore, our data revealed that inhibition of MLCK attenuated the TNFalpha induced IkappaB phosphorylation, translocation of p65, NFkappaB-DNA binding, and NFkappaB transcriptional activity ... Molecular approaches to either reduce EC MLCK expression ( AdV EC MLCK antisense construct ) or to reduce kinase activity ( kinase-dead EC MLCK ATPdel mutant ) produced similar attenuation of the TNFalpha induced NFkappaB response ... Together, these novel observations indicate that TNFalpha induced cytoskeletal rearrangement driven by MLCK activity is necessary for TNFalpha dependent NFkappaB activation and amplification of pro-survival signals
Damiano et al., J Immunol 2004 : NAIP CIIA HET-E and TP1 (NACHT) family proteins are involved in sensing intracellular pathogens or pathogen derived molecules, triggering host defense responses resulting in caspase mediated processing of proinflammatory cytokines and NF-kappaB activation
Chang et al., Mol Cells 2004 (Inflammation...) : Activation of NF-kappaB leads to the production of proinflammatory cytokines such as IL-12 and TNF-alpha that are involved in innate and adaptive immunity ... Activation of NF-kappaB leads to the production of proinflammatory cytokines such as IL-12 and TNF-alpha that are involved in innate and adaptive immunity
Kwon et al., Mol Cells 2004 : IKKgamma inhibits activation of NF-kappaB by NIK ... Our results suggest that overexpression of IKKgamma inhibits activation of NF-kappaB by NIK by competing with NIK for interaction with IKKbeta
Agarwal et al., Arthritis Rheum 2004 (Arthritis) : CTS of low magnitudes ( 4-8 % equibiaxial strain ) was a potent inhibitor of IL-1beta dependent NF-kappaB nuclear translocation
Martin et al., Arthritis Rheum 2004 (Anoxia) : IL-1beta induced DNA binding of NF-kappaB and AP-1 was significantly higher in hypoxic and reoxygenated cultures than in normoxia
Ha et al., Exp Cell Res 2004 : Pretreatment of osteoclasts with the antioxidants N-acetyl-l-cystein and glutathione reduced RANKL induced Akt, NF-kappaB , and ERK activation ... In contrast, antioxidants did not prevent TNF-alpha induced Akt and NF-kappaB activation
Ishiguro et al., Exp Cell Res 2004 : WAVE2 overexpression did not activate NF-kappaB
Li et al., J Biol Chem 2005 : We found that TCR/CD28 costimulation activates the canonical but not the non-canonical NF-kappaB pathway and that the serine/threonine kinase protein kinase C ( PKC ) is essential for TCR/CD28 mediated canonical NF-kappaB activation in T cells ... We found that TCR/CD28 costimulation activates the canonical but not the non-canonical NF-kappaB pathway and that the serine/threonine kinase protein kinase C ( PKC ) is essential for TCR/CD28 mediated canonical NF-kappaB activation in T cells
Sousa et al., FASEB J 2005 (Amyloid Neuropathies, Familial) : Biglycan , NGAL, and MMP-9 are transcriptionally up-regulated by NF-kappaB , a transcription factor that is activated in FAP nerves and SG ... Biglycan, NGAL , and MMP-9 are transcriptionally up-regulated by NF-kappaB , a transcription factor that is activated in FAP nerves and SG ... Biglycan, NGAL, and MMP-9 are transcriptionally up-regulated by NF-kappaB , a transcription factor that is activated in FAP nerves and SG
Pham et al., Cell 2004 (Inflammation) : Ferritin heavy chain upregulation by NF-kappaB inhibits TNFalpha induced apoptosis by suppressing reactive oxygen species
Suominen et al., Eur J Endocrinol 2004 : Rat seminiferous tubule segments were cultured in the presence and absence of TNF-alpha , infliximab and SN50, a NF-kappa B inhibitor
Grech et al., Immunity 2004 : To examine the role of the TNF-R superfamily signaling protein TRAF2 in mature B cell development and NF-kappaB activation, conditionally TRAF2-deficient mice were produced
Cannons et al., Immunity 2004 : SAP regulates T ( H ) 2 differentiation and PKC-theta mediated activation of NF-kappaB1
Kiela et al., Am J Physiol Gastrointest Liver Physiol 2005 (Colitis...) : Moreover, individual boswellic acids were demonstrated to increase the basal and IL-1beta stimulated NF-kappaB activity in intestinal epithelial cells in vitro as well as reverse proliferative effects of IL-1beta
Huber et al., Cell cycle (Georgetown, Tex.) 2004 : In a combined in vitro/in vivo model of mammary carcinogenesis, NF-kappaB was essential both for the induction and maintenance of EMT and for in vivo metastasis
Hosokawa et al., Int J Hematol 2004 (Lymphoma, B-Cell, Marginal Zone) : Recent genetic and biochemical studies have indicated that BCL10 and MALT1 form a physical and functional complex and are both essential for nuclear factor kappaB (NF-kappaB) activation by antigen receptor stimulation in lymphocytes ... Recent genetic and biochemical studies have indicated that BCL10 and MALT1 form a physical and functional complex and are both essential for nuclear factor kappaB (NF-kappaB) activation by antigen receptor stimulation in lymphocytes ... BCL10/MALT1- and API2-MALT1 induced NF-kappaB activation can be assumed to be able to contribute to antiapoptosis, probably through NF-kappaB mediated up-regulation of several apoptotic inhibitor genes ... BCL10/MALT1- and API2-MALT1 induced NF-kappaB activation can be assumed to be able to contribute to antiapoptosis, probably through NF-kappaB mediated up-regulation of several apoptotic inhibitor genes
Hoffman et al., Lancet 2004 (Acute Disease...) : Involvement of cryopyrin in activation of caspase 1 and NF-kappaB signalling suggests that it might have a role in many chronic inflammatory diseases
Lin et al., Semin Immunol 2004 (Lymphoma, B-Cell, Marginal Zone) : Recent studies demonstrate that CARMA1, a scaffold protein, plays an essential role in mediating TCR- or BCR induced NF-kappaB activation by recruiting two adaptor proteins, Bcl10 and MALT1, to lipid rafts following stimulation of antigen receptors
Fessler et al., J Virol 2004 : Previous findings from this laboratory demonstrated that the E3 proteins 10.4K and 14.5K, which form a complex in the plasma membrane, inhibit tumor necrosis factor (TNF) induced activation of NF-kappaB and the synthesis of chemokines
Deng et al., Mol Cell Biol 2004 : It has been noted that conditions associated with eIF2alpha phosphorylation, notably accumulation of unfolded proteins in the endoplasmic reticulum ( ER ), or ER stress, are also associated with activation of nuclear factor kappa B (NF-kappaB) and that eIF2alpha phosphorylation is required for NF-kappaB activation by ER stress ... We have used a pharmacologically activable version of pancreatic ER kinase ( PERK, an ER stress-responsive eIF2alpha kinase ) to uncouple eIF2alpha phosphorylation from stress and found that phosphorylation of eIF2alpha is both necessary and sufficient to activate both NF-kappaB DNA binding and an NF-kappaB reporter gene
Nasr et al., Oncogene 2005 (Leukemia-Lymphoma, Adult T-Cell...) : PS-341 treatment of these cells stabilized IkappaBalpha, IkappaBbeta, IkappaBvarepsilon, p21, p27 and p53 proteins and selectively inhibited Rel-A DNA binding NF-kappaB complexes ... PS-341 treatment of these cells stabilized IkappaBalpha, IkappaBbeta, IkappaBvarepsilon, p21 , p27 and p53 proteins and selectively inhibited Rel-A DNA binding NF-kappaB complexes ... PS-341 treatment of these cells stabilized IkappaBalpha, IkappaBbeta, IkappaBvarepsilon, p21, p27 and p53 proteins and selectively inhibited Rel-A DNA binding NF-kappaB complexes ... PS-341 treatment of these cells stabilized IkappaBalpha , IkappaBbeta, IkappaBvarepsilon, p21, p27 and p53 proteins and selectively inhibited Rel-A DNA binding NF-kappaB complexes ... PS-341 treatment of these cells stabilized IkappaBalpha, IkappaBbeta , IkappaBvarepsilon, p21, p27 and p53 proteins and selectively inhibited Rel-A DNA binding NF-kappaB complexes
Díaz et al., J Cell Biochem 2005 : Interestingly, IkappaBalpha , an inhibitor of NFkappaB , decreased after 24 h of treatment, suggesting further activation of the transcription factor
Peng et al., FASEB J 2005 : Furthermore, LPS induced NF-kappaB activation was decreased by inhibition of NADH oxidase, ERK1/2 or JNK1/2 activation, suggesting that LPS increases NF-kappaB activity and COX-2 expression via NADH oxidase dependent activation of ERK1/2 and JNK1/2
Shin et al., Biochem Pharmacol 2004 : Furthermore, CM inhibited the nuclear factor-kappaB (NF-kappaB) activation induced by LPS , which was associated with the prevention of the degradation of the inhibitor kappaB, and subsequently with decreased p65 protein levels in the nucleus
Panopoulos et al., J Biol Chem 2005 (Inflammation) : l-Mimosine did not affect IKK and NF-kappaB activation by IL-1beta
Novitskiy et al., Alcohol Alcohol 2005 : NF-kappaB , an inhibitor of the type I collagen promoters, is increased by both acetaldehyde and TNFalpha ... This study determined the effects of acetaldehyde in comparison to the effects of TNFalpha on inhibitory kappa B-alpha ( IkappaB-alpha ) protein and NF-kappaB activation in hepatic stellate cells ... Both acetaldehyde and TNFalpha enhanced nuclear NF-kappaB ( p65 ), but acetaldehyde alone also increased NF-kappaB ( p50 ) ... TNFalpha and acetaldehyde independently activate NF-kappaB by rapid enhancement of IkappaB-alpha kinase activity and degradation of IkB-alpha protein
Lee et al., FASEB J 2005 (Hemorrhage...) : This effect is explained in part by a HO-1 dependent attenuation of the LPS mediated nuclear translocation of NF-kappaB
Chen et al., Sheng Wu Yi Xue Gong Cheng Xue Za Zhi 2004 (Colitis, Ulcerative) : Glucocorticoids and SASP markedly inhibited NF-kappaB activation and significantly decreased mRNA and protein expression of ICAM-1 and VCAM-1 ( P < 0.05 )
Min et al., Arch Pharm Res 2004 : In the mechanism of the anti-inflammatory action, JSH-21 exhibited inhibitory effects on LPS induced DNA binding activity and transcriptional activity of nuclear factor-kappa B (NF-kappaB) ... Structural analogs of JSH-21 also inhibited both the LPS induced NO production and NF-kappaB transcriptional activity, where diamine substitution at positions 1 and 2 of JSH-21 seems to play an important role in the anti-inflammatory activity
Son et al., Arch Pharm Res 2004 (Brain Neoplasms...) : Vitamin C blocks TNF-alpha induced NF-kappaB activation and ICAM-1 expression in human neuroblastoma cells ... Moreover, a gel shift analysis indicated that vitamin C dose-dependently inhibited the NF-kappaB activation and IkappaBalpha degradation induced by TNF-alpha
Du et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2004 : NF-kappaB p65 activity induced by insulin in mesangial cells was inhibited by culture superatants of splenic lymphocytes from oral immune tolerance rats ( P < 0.05 ) ... NF-kappaB p65 activity in mesangial cells may be inhibited by IL-10 secreted by splenic lymphocytes from the oral immune tolerance rats
Wang et al., J Surg Res 2004 : The present studies were performed to identify whether the reduction of albumin expression induced by LPS was mediated by activating nuclear factor kappa B(NF-kappaB) in cultured rat hepatocytes ... LPS dramatically decreased albumin expression and enhanced NF-kappaB activity in rat hepatocytes, especially in the 1 microg/ml LPS group ... The results suggest that LPS inhibits albumin expression by activating NF-kappaB signaling
Campbell et al., J Immunol 2004 (Arthritis, Rheumatoid) : This is mediated through p38 MAPK regulation of NF-kappaB ... This is mediated through p38 MAPK regulation of NF-kappaB
Hyakushima et al., J Immunol 2004 : The addition of sTLR4 to the medium containing the MD-2 protein significantly attenuated LPS induced NF-kappaB activation and IL-8 secretion in wild-type TLR4 expressing cells
Anwar et al., J Immunol 2004 : Blockade of thrombin induced ICAM-1 expression was secondary to inhibition of NF-kappaB activity, the key regulator of ICAM-1 expression in endothelial cells ... In parallel studies we observed that inhibition of the RhoA/ROCK pathway by the same pharmacological and genetic approaches failed to inhibit TNF-alpha induced NF-kappaB activation and ICAM-1 expression
Renault et al., J Biol Chem 2005 : Supershift electrophoretic mobility shift assay and chromatin immunoprecipitation assays identified NFkappaB and USF-1/USF-2 as the DNA binding proteins induced by UTP , respectively, for these two sites ... Using dominant negative mutants of IkappaB kinase and USF transcription factors, we confirmed that NFkappaB and USF-1/USF-2 are involved in the UTP mediated expression of OPN
Schmid et al., Infect Immun 2004 (MAP Kinase Signaling System) : The Inv triggered beta1 integrin signaling involves the small GTPase Rac ; the activation of MAP kinases, such as p38 , MEK1, and JNK ; and the activation of the transcription factor NF-kappaB ... The Inv triggered beta1 integrin signaling involves the small GTPase Rac ; the activation of MAP kinases, such as p38, MEK1 , and JNK ; and the activation of the transcription factor NF-kappaB
Shirasugi et al., Transplant Proc 2004 : A virally induced alloreactive memory seems to represent a potent barrier to tolerance induction but the combination of 15-deoxyspergualin (DSG) , an inhibitor of NFkB translocation, with costimulation blockade ( CB ) -based chimerism as an induction regimen can overcome a preformed anti-donor memory response
Basak et al., J Biol Chem 2005 (MAP Kinase Signaling System) : We demonstrate that both the NF-kappaB and the C/EBPbeta binding elements of the IL-1beta promoter drive LPS induced IL-1beta gene expression
Zhang et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2004 (Burkitt Lymphoma) : [ BAY11-7082 and Lactacystein in CD154 induced NF-kappaB activation ] ... To investigate the inhibition mechanisms of BAY11-7082 ( IkappaB-alpha phosphorylation inhibitor ) and Lactacystein ( proteosome inhibitor ) in CD154 induced NF-kappaB activation ... We used recombinant CD154 to stimulate EBV/LMP1 negative Ramos B cell and observed the effects of BAY11-7082 and Lactacystein in CD154 induced NF-kappaB luciferase activation , phosphorylation and degradation of IkappaB-alpha, phosphorylation of p65, and nuclear translocation of NF-kappaB subunits upon CD154 stimulation ... We used recombinant CD154 to stimulate EBV/LMP1 negative Ramos B cell and observed the effects of BAY11-7082 and Lactacystein in CD154 induced NF-kappaB luciferase activation , phosphorylation and degradation of IkappaB-alpha , phosphorylation of p65, and nuclear translocation of NF-kappaB subunits upon CD154 stimulation ... Both BAY11-7082 and Lactacystein abrogated CD154 induced NF-kappaB luciferase activation in Ramos cells ... BAY11-7082 and Lactacystein inhibit CD154 induced NF-kappaB activation through different mechanisms
Kajita et al., Diabetes Res Clin Pract 2004 : Furthermore, TNFalpha activated nuclear factor-kappaB (NF-kappaB) , known as a downstream effector of PKCzeta to 256.6 %, which was enhanced with overexpression of wild-type PKCzeta
Everly et al., J Virol 2004 (Cell Transformation, Viral) : Latent membrane protein 1 (LMP1) , the Epstein-Barr virus oncoprotein, activates NF-kappaB , phosphatidylinositol 3-kinase, mitogen activated protein kinase, and c-Jun N-terminal kinase signaling
Gregory et al., J Virol 2004 : Absence of either IKK1 or IKK2 resulted in an 86 to 94 % loss of virus yield compared to that of normal MEFs, little or no loss of IkappaBalpha, and greatly reduced NFkappaB nuclear translocation ... Absence of either IKK1 or IKK2 resulted in an 86 to 94 % loss of virus yield compared to that of normal MEFs, little or no loss of IkappaBalpha, and greatly reduced NFkappaB nuclear translocation
Rodríguez-Iturbe et al., Am J Nephrol 2004 (Hypertension, Renal) : Tubulointerstitial accumulation of lymphocytes, macrophages, angiotensin II-positive cells, cells expressing the p65 DNA binding subunit of NF-kappaB and activation of NF-kappaB in the kidney were all significantly increased ( p < 0.01 ) in the prehypertensive SHR-3 wk group and augmented progressively, with the highest values in the SHR-24 wk group
Siendones et al., Hepatology 2004 : In conclusion, administration of PGE1 to hepatocytes produces low levels of NO, which inhibits its own formation during D-GalN induced cell death through the attenuation of NF-kappaB dependent NOS-2 expression
Chang et al., Cell Signal 2005 : PMA induced COX-2 expression was attenuated by PKC inhibitors ( Go 6976 and Ro 31-8220 ), a Ras inhibitor ( manumycin A ), a Raf-1 inhibitor ( GW 5074 ), a MEK inhibitor ( PD 098059 ), and an NF-kappaB inhibitor ( PDTC ), but not by a tyrosine kinase inhibitor ( genistein ) or a p38 MAPK inhibitor ( SB 203580 ) ... Taken together, these results indicate that PMA might activate PKC to elicit activation of the Ras/Raf-1/ERK1/2 pathway, which in turn initiates NF-kappaB activation, and finally induces COX-2 expression and PGE2 release in A549 cells
Asehnoune et al., Cell Signal 2005 : PKCalpha/beta can regulate NF-kappaB dependent transcription in neutrophils both by enhancing nuclear translocation of NF-kappaB and also by stimulating phosphorylation of the p65 subunit
Zhu et al., Biochem Biophys Res Commun 2005 : RANK-L induces the expression of NFATc1, but not of NFkappaB subunits during osteoclast formation
Mabuchi et al., Clin Cancer Res 2004 (Ovarian Neoplasms) : Inhibition of NFkappaB activity by treatment with an IkappaBalpha phosphorylation inhibitor ( BAY 11-7085 ) attenuated both basal and transient induction of IkappaBalpha phosphorylation by paclitaxel
Pivarcsi et al., Acta Microbiol Immunol Hung 2004 : In response to challenge with microbes or microbial derived substances the activation and nuclear translocation of NF-kappaB , the production of nitric oxide ( NO ) and inflammatory cytokines occur in keratinocytes, in a TLR dependent manner
Till et al., J Biol Chem 2005 : Albeit the mutation does not affect the TNF binding kinetics of TNFR2, the present study provides evidence that the mutation results in a significantly lower capability to induce TNFR2 mediated NF-kappaB activation
Blonska et al., Mol Cell Biol 2004 : Receptor interacting protein ( RIP ) plays a critical role in tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation ... We found that TNF-alpha induced NF-kappaB activation was fully restored by MEKK3-DD in these cells ... We found that TNF-alpha induced NF-kappaB activation was fully restored by MEKK3-DD in these cells ... In contrast, expression of a fusion protein composed of NEMO, a component of the IkappaB kinase complex, and the death domain of RIP ( NEMO-DD ) can not restore TNF-alpha induced NF-kappaB activation in RIP-deficient cells ... These results indicate that the role of RIP is to specifically recruit MEKK3 to the TNF-alpha receptor complex, whereas the forced recruitment of NEMO to the TNF-alpha receptor complex is insufficient for TNF-alpha induced NF-kappaB activation ... Although MEKK2 has a high degree of homology with MEKK3, MEKK2-DD, unlike MEKK3-DD, also fails to restore TNF-alpha induced NF-kappaB activation in RIP-deficient cells, indicating that RIP dependent recruitment of MEKK3 plays a specific role in TNF-alpha signaling
Lan et al., J Helminthol 2004 (Brain Diseases...) : These data suggest that the development of brain injury by eosinophilia of A. cantonensis infection is associated with activation of JAK/STAT1 signals by cytokines, and/or activation of MEKK1/JNK by oxidant stress, and/or activation of NF-kappaB by increasing IAP expression
Sattler et al., Stroke 2005 (Carotid Stenosis) : The local renin-angiotensin system (RAS) and cyclooxygenase-2 contribute to the activation of nuclear factor kappaB (NFkappaB) and C-reactive protein (CRP) ... The local renin-angiotensin system (RAS) and cyclooxygenase-2 contribute to the activation of nuclear factor kappaB (NFkappaB) and C-reactive protein (CRP)
Kanda et al., J Allergy Clin Immunol 2004 : NF-kappaB p50 or p65 antisense oligonucleotides suppressed TNF-alpha induced CCL27 production, indicating the requirement of NF-kappaB for CCL27 production
Jain et al., J Clin Invest 2004 (Ectodermal Dysplasia...) : This suggests that these NF-kappaB components have different activation requirements and that IL-4 can augment some but not all NEMO dependent NF-kappaB signaling ... This suggests that these NF-kappaB components have different activation requirements and that IL-4 can augment some but not all NEMO dependent NF-kappaB signaling
Karlsen et al., Diabetologia 2004 : Multiple IL-1beta induced NF-kappaB dependent proapoptotic early response genes were inhibited by SOCS-3 expression, suggesting that SOCS-3 inhibits NF-kappaB mediated signalling
Kuo et al., J Trauma 2004 (Reperfusion Injury) : GSNO significantly inhibited superoxide production and suppressed NF-kappa B activation, iNOS induction , and 3-nitrotyrosine expression, but up-regulated endothelial NOS expression in the flap vessels
Carvalho et al., Oncogene 2005 (Carcinoma, Renal Cell...) : Inhibition of JAK2, one of the receptor-proximal elements of EPO mediated signal transduction, greatly diminished the EPO mediated chemosensitization and NF-kappaB inhibition ... Inhibition of JAK2 , one of the receptor-proximal elements of EPO mediated signal transduction, greatly diminished the EPO mediated chemosensitization and NF-kappaB inhibition ... EPO lost its death facilitating effects in the presence of an NF-kappaB inhibitor, underscoring the cause-effect relationship between EPO mediated chemosensitization and NF-kappaB inhibition
Minguet et al., Eur J Immunol 2005 (MAP Kinase Signaling System) : Anergic B lymphocytes exert compromised signal transduction towards the activation of NF-kappa B in response to B cell antigen receptor (BCR) triggering, whereas activation of the ERK pathway appears normal
Spiecker et al., Arch Biochem Biophys 2005 (Inflammation) : CYP2J2 possesses vascular protective effects, which include but are not limited to, protection against ischemia-reperfusion injury, suppression of reactive oxygen species following hypoxia-reoxygenation, inhibition of the pro-inflammatory transcription factor , nuclear factor-kappaB (NF-kappaB) , attenuation of vascular smooth muscle migration, and enhancement of a fibrinolytic pathway
Kabashima et al., FEBS Lett 2004 (Hypersensitivity, Delayed...) : Here, we found that our recently synthesized NF-kappaB inhibitor attenuated an ovalbumin-specific delayed-type hypersensitivity response in vivo and suppressed production of IL-12 by dendritic cells and TH1 cytokines by T cells in vitro
Djavaheri-Mergny et al., FEBS Lett 2004 (Sarcoma, Ewing) : As oxidative stress is one of the most prominent activators of JNK, we investigated the relationship between TNFalpha induced NF-kappaB activation and the control of oxidative stress
Leclercq et al., J Hepatol 2004 (Choline Deficiency...) : Curcumin prevented the MCD induced activation of NF-kappaB and decreased downstream induction of ICAM-1, COX-2 and MCP-1
Kim et al., Biochem Biophys Res Commun 2005 : Electrophoretic mobility shift assays showed that both Taxol and LPS rapidly activated identical NF-kappaB complexes that could bind to the iNOS promoter
Takenaka et al., Microbiology 2004 (Inflammation) : These findings suggest that H. pylori HSP60 activates NF-kappaB and induces IL-8 production through TLR triggered pathways in gastric epithelial cells
Uchiba et al., Thromb Haemost 2004 : These observations suggested that AT inhibited the TNF-alpha induced increase in E-selectin expression in HUVECs by inhibiting the interaction of NF-kappaB with CBP/p300 through cAMP dependent protein kinase A-induced CREB activation
Zhang et al., Int J Mol Med 2005 : A blockade of NF-kappaB activation by the overexpression of a stable form of IkappaBalpha completely blocked these responses, indicating that the induction of p19 mRNA expression by IL-1beta and TNF-alpha was mediated by the NF-kappaB activation pathway
Esparza et al., Cell Mol Life Sci 2004 : Further analyses revealed that TRAF4 mediated NF-kappaB activation downstream of GITR depends on a previously mapped TRAF binding site in the cytoplasmic domain of the receptor and is inhibited by the cytoplasmic protein A20
Jin et al., Phytochemistry 2004 : Inhibitors of the LPS induced NF-kappaB activation from Artemisia sylvatica ... All isolated compounds displayed inhibitory activity on the LPS induced NF-kappaB activation , NO production, and TNF-alpha production with IC50 values of 0.49-7.17, 1.46-6.16, and 3.19-27.76 microM, respectively, in RAW264.7 cells
Sacre et al., J Endotoxin Res 2004 : In cell lines and murine models, parts of the signaling pathways involved have been elucidated with MyD88, Mal/TIRAP and IKK2 playing an important role in the induction of NF-kappaB ... In cell lines and murine models, parts of the signaling pathways involved have been elucidated with MyD88, Mal/TIRAP and IKK2 playing an important role in the induction of NF-kappaB ... In cell lines and murine models, parts of the signaling pathways involved have been elucidated with MyD88, Mal/TIRAP and IKK2 playing an important role in the induction of NF-kappaB ... In cell lines and murine models, parts of the signaling pathways involved have been elucidated with MyD88 , Mal/TIRAP and IKK2 playing an important role in the induction of NF-kappaB ... We show that in primary human macrophages MyD88, Mal/TIRAP and IKK2 independent alternative pathways activate NF-kappaB and induce the expression of inflammatory cytokines, whereas in non-myeloid synovial fibroblasts MyD88 and/or Mal/TIRAP are essential adaptors for LPS signaling ... We show that in primary human macrophages MyD88, Mal/TIRAP and IKK2 independent alternative pathways activate NF-kappaB and induce the expression of inflammatory cytokines, whereas in non-myeloid synovial fibroblasts MyD88 and/or Mal/TIRAP are essential adaptors for LPS signaling ... We show that in primary human macrophages MyD88 , Mal/TIRAP and IKK2 independent alternative pathways activate NF-kappaB and induce the expression of inflammatory cytokines, whereas in non-myeloid synovial fibroblasts MyD88 and/or Mal/TIRAP are essential adaptors for LPS signaling
Lee et al., J Ethnopharmacol 2005 (Dermatitis, Atopic) : BSASM inhibited LPS induced activation of NF-kappaB promoter
Gruber et al., Mol Immunol 2005 : Consistently, after CD3/CD28 engagement, deficiency of PKCepsilon did not impair NF-kappaB transactivation as well as CD25, CD44 and CD69 induction ... Consistently, after CD3/CD28 engagement, deficiency of PKCepsilon did not impair NF-kappaB transactivation as well as CD25, CD44 and CD69 induction ... Consistently, after CD3/CD28 engagement, deficiency of PKCepsilon did not impair NF-kappaB transactivation as well as CD25 , CD44 and CD69 induction
Kim et al., Int Immunopharmacol 2005 : In addition, luteolin inhibited TNF-alpha induced phosphorylation of p38 MAPK and extracellular regulated kinases (ERK), IkappaB degradation, and NF-kappaB activation
Lamkanfi et al., J Biol Chem 2005 : Caspase-2 , unlike caspase-3, -6, -7, -9, -11, -12, and -14, is a potent inducer of NF-kappaB and p38 MAPK activation in a TRAF2 mediated way ... Structure-function analysis shows that NF-kappaB activation occurs independent of enzymatic activity of the protease and that the caspase recruitment domain of caspase-2 is sufficient for the activation of NF-kappaB and p38 MAPK
Powell et al., J Mol Endocrinol 2004 : Inhibition of NFkappaB activity decreased, but did not completely inhibit , cytokine induced iNOS promoter activity and subsequent production of NO
Manna et al., J Biol Chem 2005 : In this report we provide evidence that like TNF, an inducer of NF-kappaB and also a NF-kappaB dependent gene product, IL-8 induces NF-kappaB in a unique pathway ... IL-8 induces NF-kappaB activation in a dose dependent manner in different cell types as detected by a DNA-protein binding assay ... IL-8 induces NF-kappaB dependent reporter gene expression as well as ICAM-1, VCAM-1 , and Cox-2 expression ... IL-8 induces NF-kappaB dependent reporter gene expression as well as ICAM-1 , VCAM-1, and Cox-2 expression ... IL-8 induces NF-kappaB dependent reporter gene expression as well as ICAM-1, VCAM-1, and Cox-2 expression ... IL-8 induces NF-kappaB dependent reporter gene expression as well as ICAM-1, VCAM-1, and Cox-2 expression ... IL-8 induced NF-kappaB activation is for the most part unaltered when cells are transfected with dominant negative TRADD, FADD, or TRAF2, but is inhibited with dominant negative TRAF6-, NIK-, IKK-, or IkappaBalpha transfected cells ... The data suggest that IL-8 induced NF-kappaB activation proceeds through a TRAF2 independent but TRAF6 dependent pathway, followed by recruitment of IRAK and activation of IKK ... IL-8 induced NF-kappaB activation is not observed in a cell-permeable peptide that has TRAF6 binding motif treated cells or IRAK-deficient cells ... IL-8 induced NF-kappaB activation proceeds mostly through interaction with TRAF6 and partially through the Rho-GTPase pathways ... This is the first report that IL-8 induces NF-kappaB in a distinct pathway, and activation of NF-kappaB and its dependent genes may be one of the pathways of IL-8 induced inflammation and angiogenesis
Yu et al., Blood 2005 (Arteriosclerosis) : Endothelial expression of E-selectin is induced by the platelet-specific chemokine platelet factor 4 through LRP in an NF-kappaB dependent manner ... Endothelial expression of E-selectin is induced by the platelet-specific chemokine platelet factor 4 through LRP in an NF-kappaB dependent manner ... Endothelial expression of E-selectin is induced by the platelet-specific chemokine platelet factor 4 through LRP in an NF-kappaB dependent manner
Felli et al., Oncogene 2005 (Leukemia, T-Cell...) : We previously showed that overexpression of pT alpha/pre-TCR and constitutive activation of NF-kappa B characterize the T-cell leukemia/lymphoma developing in Notch3-IC transgenic mice
Agarwal et al., Oncogene 2005 (Colorectal Neoplasms...) : Inducible expression of either KD IKK alpha or WT PTEN strongly inhibits both the constitutive NF kappa B- and beta-catenin dependent promoter and endogenous gene activation ... Inducible expression of either KD IKK alpha or WT PTEN strongly inhibits both the constitutive NF kappa B- and beta-catenin dependent promoter and endogenous gene activation
Jun et al., Oncogene 2005 : Role of FLASH in caspase-8 mediated activation of NF-kappaB : dominant negative function of FLASH mutant in NF-kappaB signaling pathway ... Here, we addressed the role of endogenous caspase-8 in tumor necrosis factor (TNF)-alpha induced activation of NF-kappaB ... Here, we addressed the role of endogenous caspase-8 in tumor necrosis factor (TNF)-alpha induced activation of NF-kappaB ... Direct targeting of caspase-8 with siRNA and antisense ( AS ) approaches abolished TNF-alpha induced activation of NF-kappaB in NIH3T3, HeLa, and HEK293 cells as determined with luciferase reporter gene and cell fractionation assays ... Instead, caspase-8 ( D73A ) mutant bound to caspase-8 and blocked NF-kappaB activation triggered by TNF-alpha and caspase-8 ... Taken together, these results suggest that endogenous caspase-8 mediates TNF-alpha induced activation of NF-kappaB via FLASH ... Taken together, these results suggest that endogenous caspase-8 mediates TNF-alpha induced activation of NF-kappaB via FLASH
Xiao et al., J Zhejiang Univ Sci B 2005 (Brain Edema...) : Compared with Group B, NF-kappaB activation ( P < 0.01 ), the levels of TNF-alpha protein ( P < 0.01 ) and the water content of brain ( P < 0.05 ) began to decrease obviously after injury in Groups C and D. Beta-Aescin could dramatically inhibit NF-kappaB activation and the expression of TNF-alpha protein in the rat brain, alleviate rat brain edema, and that could partially be the molecular mechanism by which beta-Aescin attenuates traumatic brain edema
Fujihara et al., Biochem Biophys Res Commun 2005 : TAT-I kappa B alpha ( S32A, S36A ) inhibited NF-kappa B-dependent transcription in HeLa and A549 cells by retaining NF-kappa B p65 in the cytoplasm ... TAT-I kappa B alpha ( S32A, S36A ) inhibited NF-kappa B-dependent transcription in HeLa and A549 cells by retaining NF-kappa B p65 in the cytoplasm
Kang et al., Cancer Lett 2005 (Neoplasms...) : Cyclooxygenase-2 (COX-2) expression is mediated by constitutive nuclear factor (NF)-kappaB
Routes et al., J Virol 2005 : Like E1A, E6 has been reported to sensitize cells to lysis by TNF-alpha by inhibiting the TNF-alpha induced activation of NF-kappaB ... We found that E1A, but not E6, blocked the TNF-alpha induced activation of NF-kappaB , an activity that correlated with E1A-p300 binding
Vestergaard et al., Cytokine 2005 : We show here that inhibition of NF-kappaB with the non-specific NF-kappaB inhibitors SSC ( sodium salicylate ), DCIC ( 3,4-dichloroisocoumarin ) and PAO ( phenylarsine oxide ) results in a downregulation of TNF-alpha induced CCL27 production
Ho et al., Blood 2005 (Lymphoma, B-Cell...) : API2-MALT1 and exogenous MALT1 increased constitutive NF-kappa B activity and enhanced I kappa B kinase (IKK) activation induced by CD40 stimulation ... API2-MALT1 and exogenous MALT1 increased constitutive NF-kappa B activity and enhanced I kappa B kinase (IKK) activation induced by CD40 stimulation
Wu et al., J Card Fail 2004 (Anoxia) : Furthermore, transfection assay using HO-1 promoter constructs revealed the involvement of the nuclear factor kB (NF-kB) and Activator protein-1 (AP-1) in the hypoxia induced activation of the HO-1 gene
Starace et al., FASEB J 2005 : We studied the role of the transcription factor NF-kappaB and of MAPKs in regulating Fas expression
Cheng et al., Mol Cell Biol 2005 (Osteosarcoma) : hTid-1 , a human homolog of the Drosophila tumor suppressor l ( 2 ) Tid and a novel DnaJ protein, regulates the activity of nuclear factor kappaB (NF-kappaB) , but its mechanism is not established ... Collectively, the data suggest that hTid-1 represses the activity of NF-kappaB through physical and functional interactions with the IKK complex and IkappaB and, in doing so, it modulates cell growth and death
Dheen et al., Glia 2005 (Neurodegenerative Diseases) : The anti-inflammatory effects of RA were correlated with the enhanced expression of retinoic acid receptor-beta, and transforming growth factor-beta1 as well as the inhibition of NF-kappaB translocation
Vakkila et al., J Immunol Methods 2004 : IFNgamma amplified IL-1 induced NF-kappaB responses ... NF-kappaB responses to IL-1beta, CD40L , and LPS were donor dependent ( n=7 ), correlated with the quality of iDC preparations ( p=0.002 ), and IL-12 p70 production ( p=0.010 ) ... NF-kappaB responses to IL-1beta, CD40L , and LPS were donor dependent ( n=7 ), correlated with the quality of iDC preparations ( p=0.002 ), and IL-12 p70 production ( p=0.010 ) ... NF-kappaB responses to IL-1beta , CD40L, and LPS were donor dependent ( n=7 ), correlated with the quality of iDC preparations ( p=0.002 ), and IL-12 p70 production ( p=0.010 ) ... NF-kappaB responses to IL-1beta, CD40L, and LPS were donor dependent ( n=7 ), correlated with the quality of iDC preparations ( p=0.002 ), and IL-12 p70 production ( p=0.010 )
Zhou et al., Cancer Res 2004 (MAP Kinase Signaling System) : In tubular LLC-PK ( 1 ) cells, activation of nuclear factor kappaB (NFkappaB) by TNF-alpha resulted in HIF-1alpha protein synthesis as determined by [ ( 35 ) S ] methionine pulse experiments ... In tubular LLC-PK ( 1 ) cells, activation of nuclear factor kappaB (NFkappaB) by TNF-alpha resulted in HIF-1alpha protein synthesis as determined by [ ( 35 ) S ] methionine pulse experiments
Li et al., Free Radic Biol Med 2005 (Cardiomegaly) : Our results revealed that pretreatment with ISO significantly inhibited Ang II-mediated NF-kappaB through affecting the degradation and phosphorylation of IkappaBalpha and the activity of IKKbeta and AP-1 activation by influencing the expression of c-Fos and c-Jun proteins ... Our results revealed that pretreatment with ISO significantly inhibited Ang II-mediated NF-kappaB through affecting the degradation and phosphorylation of IkappaBalpha and the activity of IKKbeta and AP-1 activation by influencing the expression of c-Fos and c-Jun proteins
An et al., Oncogene 2005 (Carcinoma, Renal Cell...) : Using isogenic paired cell lines with and without expression of wtVHL, we have confirmed that VHL expression reduces constitutive NF-kappaB activity
Theuer et al., Kidney Int 2005 : EPA treatment reduced activator protein-1 (AP-1) activation and partially inhibited nuclear factor-kappaB (NF-kappaB) activity in kidneys of dTGR
Jeong et al., J Biol Chem 2005 : A novel NF-kappaB pathway involving IKKbeta and p65/RelA Ser-536 phosphorylation results in p53 Inhibition in the absence of NF-kappaB transcriptional activity ... A novel NF-kappaB pathway involving IKKbeta and p65/RelA Ser-536 phosphorylation results in p53 Inhibition in the absence of NF-kappaB transcriptional activity ... A novel NF-kappaB pathway involving IKKbeta and p65/RelA Ser-536 phosphorylation results in p53 Inhibition in the absence of NF-kappaB transcriptional activity
Tran et al., J Biol Chem 2005 (Astrocytoma...) : In addition, Fn14 activation induced BCL-XL and BCL-W mRNA and protein levels, and this effect was dependent upon NFkappaB transcriptional activity ... Our results suggest that NFkappaB mediated up-regulation of BCL-XL and BCL-W expression in glioma cells increases cellular resistance to cytotoxic therapy induced apoptosis
Li et al., J Immunol 2005 (Lymphoma, B-Cell) : We report in this study that BCL-6 can bind to three sequence motifs in the 5 ' regulatory region of NF-kappaB1 in vitro and in vivo, and repress NF-kappaB1 transcription both in reporter assays and in lymphoma B cell lines ... BCL-6 ( -/- ) mice further confirm the biological relevance of BCL-6 dependent regulation of NF-kappaB1 because BCL-6 inactivation caused notable increase in p105/p50 proteins in several cell types ... Our results demonstrate that BCL-6 can negatively regulate NF-kappaB1 expression, thereby inhibiting NF-kappaB mediated cellular functions
Lin et al., J Immunol 2005 : The expression of IL-6, IL-8, and MCP-1 was blocked by the preabsorption of anti-DV NS1 with DV NS1 or by the inhibition of NF-kappaB activation ... The expression of IL-6 , IL-8, and MCP-1 was blocked by the preabsorption of anti-DV NS1 with DV NS1 or by the inhibition of NF-kappaB activation ... The expression of IL-6, IL-8 , and MCP-1 was blocked by the preabsorption of anti-DV NS1 with DV NS1 or by the inhibition of NF-kappaB activation
Wang et al., J Immunol 2005 (Disease Models, Animal...) : Taken together, these results suggest that chronic morphine treatment in an S. pneumoniae infection model suppresses NF-kappaB gene transcription in lung resident cells, which, in turn, modulates the transcriptional regulation of MIP-2 and inflammatory cytokines
Grimm et al., Blood 2005 (Epstein-Barr Virus Infections) : BAX expression was regulated by LMP-1 activation of nuclear factor kappaB (NF-kappaB) via the C-terminal activation region 1 ( CTAR-1 ) and CTAR-2 ... BAX expression was regulated by LMP-1 activation of nuclear factor kappaB (NF-kappaB) via the C-terminal activation region 1 ( CTAR-1 ) and CTAR-2
Powers et al., Surgery 2005 (Reperfusion Injury...) : S/R with antioxidants such as N-acetylcysteine (NAC) prevents lipopolysaccharide (LPS) induced cytokine production and NF-kappaB activation in rat alveolar macrophages ... LPS induced NF-kappaB translocation in alveolar macrophages after RL was 42 % compared to 20 % after HTS ... HTS resuscitation prevents systemic oxidative stress by reducing gut ischemia/reperfusion injury and consequently attenuates distant alveolar macrophage priming, thereby reducing LPS induced NF-kappaB nuclear translocation in alveolar macrophages and organ injury
Fichorova et al., Antimicrob Agents Chemother 2005 (Vaginitis) : Within 6 h of exposure, TNF-alpha and N-9 triggered NF-kappaB and AP-1/cFos activation and upregulated interleukins and an array of chemokines by vaginal and polarized cervical epithelial cells
Thyss et al., EMBO J 2005 : Furthermore, we demonstrate that Egr-1 is induced upon UVB irradiation through NF-kappaB activation and the binding of p65/RelA within the Egr-1 promoter
Kempf et al., Cell Microbiol 2005 : The anti-apoptotic effect of B. henselae was accompanied by ( i ) the activation of the transcription factor NF-kappaB and ( ii ) the induction of cellular inhibitor of apoptosis proteins-1 and -2 ( cIAP-1 , -2 )
Clifton et al., Infect Immun 2005 : NF-kappaB activation during Rickettsia rickettsii infection of endothelial cells involves the activation of catalytic IkappaB kinases IKKalpha and IKKbeta and phosphorylation-proteolysis of the inhibitor protein IkappaBalpha ... NF-kappaB activation during Rickettsia rickettsii infection of endothelial cells involves the activation of catalytic IkappaB kinases IKKalpha and IKKbeta and phosphorylation-proteolysis of the inhibitor protein IkappaBalpha
Hamann et al., Infect Immun 2005 (Acute-Phase Reaction) : Here, we report that human high-dose LBP ( hd-LBP ) suppresses binding of both R-type and S-type LPS to CD14 and inhibits LPS induced nuclear translocation of NF-kappaB , although cellular uptake of R-type LPS was found to be increased by hd-LBP ... Here, we report that human high-dose LBP ( hd-LBP ) suppresses binding of both R-type and S-type LPS to CD14 and inhibits LPS induced nuclear translocation of NF-kappaB , although cellular uptake of R-type LPS was found to be increased by hd-LBP ... Here, we report that human high-dose LBP ( hd-LBP ) suppresses binding of both R-type and S-type LPS to CD14 and inhibits LPS induced nuclear translocation of NF-kappaB , although cellular uptake of R-type LPS was found to be increased by hd-LBP
Motoyama et al., J Biol Chem 2005 (Inflammation) : Previous studies using an NF-kappaB reporter have shown that IkappaB-zeta inhibits the activity of NF-kappaB
Wu et al., J Bone Miner Res 2005 (Bone Resorption) : The involvement of NF-kappaB in the regulation of Fas by RANKL was analyzed by luciferase assay and EMSA
Abbott et al., Curr Biol 2004 (Crohn Disease) : Lastly, we show functionally that RIP2 induced ubiquitinylation of NEMO is at least in part responsible for RIP2 mediated NF-kB activation
Zhou et al., J Clin Immunol 2004 : IL-8 induction by hyperforin required the activation of AP-1 but not the NF-kappaB transcription factor, thereby distinguishing it from the NF-kappaB dependent IL-8 induction mediated by tumor necrosis factor alpha (TNFalpha)
Naiki et al., J Biol Chem 2005 : In this study we show that TGF-beta1 blocks the NF-kappaB activation and cytokine release that is stimulated by ligands for TLRs 2, 4, and 5
Murley et al., Radiat Res 2004 : Delayed radioprotection by NFkappaB mediated induction of Sod2 ( MnSOD ) in SA-NH tumor cells after exposure to clinically used thiol containing drugs
Jeng et al., Immunol Lett 2005 : Sesamin and sesamolin also reduced LPS activated p38 mitogen activated protein kinase ( MAPK ) and nuclear factor (NF)-kappaB activations
Nojek et al., Medicina (B Aires) 2004 : We have previously shown that nuclear receptor coactivator overexpression significantly enhanced NF-kappaB activity in a dose response manner ... We determined that : 1 ) the p38 specific inhibitor reduces 50 % NF-kappaB transcriptional activity, even in cells that overexpress distinct TIF2 deletions ; 2 ) there is a physical interaction between TIF2 and p38 and RelA determined through in vitro translated protein binding assays ; 3 ) TIF2 is a p38 substrate ; 4 ) there is a physical interaction between TIF2 and IKK in TNF-alpha 20 ng/ml stimulated or not HEK 293 cell protein extract, and IkappaB only in basal conditions, determined by binding pull down assays
Walmsley et al., J Exp Med 2005 (Anoxia) : Hypoxia induced neutrophil survival is mediated by HIF-1alpha dependent NF-kappaB activity
Alikhani et al., J Biol Chem 2005 : Although TNF mediated apoptosis has been the subject of intense investigation, the transcriptional mechanisms through which it promotes apoptosis are not well understood and, paradoxically, the archetypal TNF induced nuclear factor NFkappaB is anti-apoptotic
Doerre et al., J Immunol 2005 : We propose an important role for IkappaBepsilon in the differential regulation of nuclear NF-kappaB activity in stimulated B cells
Mukundan et al., J Immunol 2005 : However, ligation of a CD40 mutant lacking a functional TRAF6 binding site did not initiate inflammatory cytokine production, and this mutant was found to be defective in CD40 mediated activation of ERK1/2, as well as IkappaB kinase (IKK) and NF-kappaB
Kim et al., Biol Pharm Bull 2005 (Edema) : Furthermore, MEXS inhibited nuclear factor kappa B (NF-kappaB) DNA binding activity and the translocation of NF-kappaB to the nucleus by blocking the degradation of inhibitor of kappa B-alpha ( IkappaB-alpha )
Woo et al., FEBS Lett 2004 : Inhibition of Bcl10 mediated activation of NF-kappa B by BinCARD , a Bcl10 interacting CARD protein ... Inhibition of Bcl10 mediated activation of NF-kappa B by BinCARD, a Bcl10 interacting CARD protein ... BinCARD potently suppressed NF-kappa B activation induced by Bcl10 and decreased the amounts of phosphorylated Bcl10 ... BinCARD potently suppressed NF-kappa B activation induced by Bcl10 and decreased the amounts of phosphorylated Bcl10 ... Mutations at the residue Leu17 or Leu65, which is highly conserved in CARD, abolished the inhibitory effects of BinCARD on both Bcl10 induced activation of NF-kappa B and phosphorylation of Bcl10 ... Mutations at the residue Leu17 or Leu65, which is highly conserved in CARD, abolished the inhibitory effects of BinCARD on both Bcl10 induced activation of NF-kappa B and phosphorylation of Bcl10 ... These results suggest that BinCARD interacts with Bcl10 to inhibit Bcl10 mediated activation of NF-kappa B and to suppress Bcl10 phosphorylation ... These results suggest that BinCARD interacts with Bcl10 to inhibit Bcl10 mediated activation of NF-kappa B and to suppress Bcl10 phosphorylation
Murakami et al., Arch Biochem Biophys 2005 : Thus, we further synthesized dehydrodiisoeugenol and alpha-diisoeugenol from isoeugenols, and investigated whether these dimers could inhibit LPS stimulated nuclear factor kappa B (NF-kappaB) activation and cyclooxygenase (COX)-2 gene expression, both of which are closely involved in inflammation and mutagenesis ... Dehydrodiisoeugenol also significantly inhibited LPS stimulated phosphorylation dependent proteolysis of inhibitor kappaB-alpha and transcriptional activity of NF-kappaB in the cells
Klooster et al., Arthritis Res Ther 2005 (MAP Kinase Signaling System) : EGF alone did not activate NF-kappaB or alter NF-kappaB activation by TNF-alpha ... EGF alone did not activate NF-kappaB or alter NF-kappaB activation by TNF-alpha
Kim et al., Arthritis Res Ther 2005 (Arthritis, Rheumatoid...) : IL-17 production by activated RA PBMC is completely or partly blocked in the presence of the NF-kappaB inhibitor pyrrolidine dithiocarbamate and the PI3K/Akt inhibitor wortmannin and LY294002, respectively
Wang et al., J Biol Chem 2005 : Overexpression of dominant negative Erk ( DN-Erk ) or pre-exposure to ERK inhibitor PD98059 inhibited NF-kappaB
Secchiero et al., Blood 2005 (Inflammation) : Unexpectedly, the antiadhesive activity of TRAIL was not due to interference with the nuclear factor kappaB (NF-kappaB) mediated up-regulation of surface intercellular adhesion molecule 1 ( ICAM-1 ), vascular cell adhesion molecule 1 ( VCAM-1 ), and E-selectin adhesion molecules in response to inflammatory cytokines ... Unexpectedly, the antiadhesive activity of TRAIL was not due to interference with the nuclear factor kappaB (NF-kappaB) mediated up-regulation of surface intercellular adhesion molecule 1 ( ICAM-1 ), vascular cell adhesion molecule 1 ( VCAM-1 ), and E-selectin adhesion molecules in response to inflammatory cytokines
Shiao et al., Br J Pharmacol 2005 : Treatment with 50 microM of falcarindiol for 30 min decreased LPS/IFN-gamma induced nuclear factor-kappaB (NF-kappaB) activation by 32 % ... Treatment with 50 microM of falcarindiol for 30 min decreased LPS/IFN-gamma induced nuclear factor-kappaB (NF-kappaB) activation by 32 %
Pradillo et al., J Cereb Blood Flow Metab 2005 (Brain Ischemia) : In summary, these data show that IPC produces neuronal upregulation of TACE and TNFR1 , and that the pathway TACE/TNF-alpha/TNFR1/NF-kappaB is involved in IT ... In summary, these data show that IPC produces neuronal upregulation of TACE and TNFR1, and that the pathway TACE/TNF-alpha/TNFR1/NF-kappaB is involved in IT
Gupta et al., Cell Death Differ 2005 : In this study, we have compared TNF-alpha induced activation of NF-kappaB , phosphorylation of IkappaBalpha, and the expression of IKKbeta between lymphocytes from young and aged humans
Cinatl et al., Int J Mol Med 2005 (Colonic Neoplasms...) : High hydrocortisone concentrations ( > or =50 microg/ml ) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10 , but did not reduce chemokine expression to basal levels ... High hydrocortisone concentrations ( > or =50 microg/ml ) completely prevented increased DNA binding activity of AP-1 and NF-kappaB and inhibited up-regulation of CXCL8 and CXCL10, but did not reduce chemokine expression to basal levels
Ji et al., Ann Clin Lab Sci 2004 (Disease Models, Animal...) : The results showed that LPS elevated the production of TNF-alpha , IL-6, and IL-10 and enhanced NF-kappaB activation in rat intestine ... The results showed that LPS elevated the production of TNF-alpha, IL-6, and IL-10 and enhanced NF-kappaB activation in rat intestine ... The results showed that LPS elevated the production of TNF-alpha, IL-6 , and IL-10 and enhanced NF-kappaB activation in rat intestine ... At all dosages, PTX reduced the activation of NF-kappaB and the production of TNF-alpha and IL-6, but enhanced the release of IL-10
Cavin et al., Biochem Biophys Res Commun 2005 : The inhibition of TNF-alpha dependent induction of COX-2 expression was mediated by an inhibition of NF-kappaB activation
Matsuda et al., J Virol 2005 (Leukemia-Lymphoma, Adult T-Cell) : Our results suggest that the constitutive activation of NF-kappaB is essential for Apo2L gene induction and protection against Apo2L induced apoptosis and that suppression of NF-kappaB may be a useful adjunct in clinical use of Apo2L against ATL
Valacchi et al., Antioxid Redox Signal 2005 (Inflammation) : Studies of nuclear factor-kappaB (NFkappaB) activation, an essential event in IL-8 production, showed decreased TNFalpha induced NFkappaB activation by acrolein, illustrated by inhibition of nuclear translocation of NFkappaB and reduced IkappaBalpha degradation ... Immunochemical analysis of IkappaB kinase (IKK) , a redox-sensitive regulator of NFkappaB activation, indicated direct modification of the IKK beta-subunit by acrolein, suggesting that acrolein may act directly on IKK
Biswas et al., Antioxid Redox Signal 2005 : Curcumin inhibited both H2O2- and TNF-alpha mediated activation of NF-kappaB and AP-1, and IL-8 release
Severa et al., Biochem Pharmacol 2005 : A marked reduction of NF-kappa B binding to the kappa B sequences present within the p35 and p40 subunit promoters was observed in diltiazem treated DCs following the stimulation with lipopolysaccharide (LPS) or CD40L ... A marked reduction of NF-kappa B binding to the kappa B sequences present within the p35 and p40 subunit promoters was observed in diltiazem treated DCs following the stimulation with lipopolysaccharide (LPS) or CD40L
Matsubara et al., Biochem Pharmacol 2005 (MAP Kinase Signaling System) : PD98059, a MEK inhibitor, and BAY 11-8702, an I kappa B-alpha inhibitor, reduced ERK and NF-kappa B cascade activation, respectively, with little effect on PKC phosphorylation
Kim et al., J Ethnopharmacol 2005 : Cheongyeolsaseuptang inhibits production of TNF-alpha , IL-6 and IL-8 as well as NF-kappa B activation in human mast cells ... Cheongyeolsaseuptang inhibits production of TNF-alpha, IL-6 and IL-8 as well as NF-kappa B activation in human mast cells ... Cheongyeolsaseuptang inhibits production of TNF-alpha, IL-6 and IL-8 as well as NF-kappa B activation in human mast cells
Fernandez-Zapico et al., Cancer Cell 2005 (Adenocarcinoma...) : Mechanistically, the effects of Vav1 require its GEF activity and the activation of Rac1, PAK1, and NF-kappaB and involve cyclin D1 upregulation ... Mechanistically, the effects of Vav1 require its GEF activity and the activation of Rac1, PAK1, and NF-kappaB and involve cyclin D1 upregulation ... Mechanistically, the effects of Vav1 require its GEF activity and the activation of Rac1, PAK1, and NF-kappaB and involve cyclin D1 upregulation
Heyninck et al., Trends Biochem Sci 2005 (Inflammation) : Recently, the underlying mechanism by which A20 downregulates NF-kappaB activation in response to the pro-inflammatory cytokine tumor necrosis factor (TNF) has been described
Opitz et al., Circ Res 2005 : In conclusion, we demonstrated that C pneumoniae induced a Nod1 mediated and Nod2 mediated NF-kappaB activation in HEK293 cells ... In conclusion, we demonstrated that C pneumoniae induced a Nod1 mediated and Nod2 mediated NF-kappaB activation in HEK293 cells
Buddenkotte et al., J Invest Dermatol 2005 (Dermatitis, Atopic) : Electrophoretic mobility shift assays and morphological transduction studies revealed PAR2 induced activation and translocation of nuclear factor kappa B (NF-kappaB) in primary human KTC with a maximum after 1 h. Supershift analysis demonstrated acivation of the p50/p65 heterodimer complex ... Use of NF-kappaB inhibitors prevented upregulation of the cell adhesion molecule ICAM-1 in KTC indicating a direct role of NF-kappaB in PAR2 mediated upregulation of ICAM-1
Cherukuri et al., Cancer Biol Ther 2005 (Colorectal Neoplasms) : The results suggested the role of nuclear factor-kappa B (NF-kappaB) in transcriptional regulation of COX-2
Kato et al., J Biol Chem 2005 (Melanoma, Experimental) : These data suggest that PLD, mitogen activated protein kinases ( extracellular signal regulated kinases 1/2 and p38 ), and NF kappa B mediate the acidic pHe signaling to induce MMP-9 expression
Li et al., J Cell Sci 2005 : Overexpression of TRIP6 potentiates RIP2 mediated NF-kappaB activation in a dose dependent manner ... Overexpression of TRIP6 potentiates NF-kappaB activation by TNF, IL-1, TLR2 or Nod1, whereas a dominant negative mutant or RNA-interference construct of TRIP6 inhibits NF-kappaB activation by TNF, IL-1, TLR2 or Nod1 ... Overexpression of TRIP6 potentiates NF-kappaB activation by TNF, IL-1, TLR2 or Nod1, whereas a dominant negative mutant or RNA-interference construct of TRIP6 inhibits NF-kappaB activation by TNF , IL-1, TLR2 or Nod1 ... Overexpression of TRIP6 potentiates NF-kappaB activation by TNF, IL-1, TLR2 or Nod1, whereas a dominant negative mutant or RNA-interference construct of TRIP6 inhibits NF-kappaB activation by TNF, IL-1, TLR2 or Nod1 ... Overexpression of TRIP6 potentiates NF-kappaB activation by TNF, IL-1, TLR2 or Nod1, whereas a dominant negative mutant or RNA-interference construct of TRIP6 inhibits NF-kappaB activation by TNF, IL-1 , TLR2 or Nod1
Zeng et al., Acta Pharmacol Sin 2005 : Inhibitors of PKC ( calphostin C, 50-500 nmol/L and RO-31-8220, 10-100 nmol/L ), CaM ( W7, 28-280 micromol/L ), ERK1/2 MAPK ( PD 98059, 2-20 micromol/L ), p38 MAPK ( SB 203580, 0.6-6 micromol/L ), JNK MAPK ( curcumin, 2-10 micromol/L ), and NF-kappaB ( PDTC, 10-100 nmol/L ) markedly reduced Hcy 100 micromol/L induced production of MCP-1 and IL-8 in human cultured whole blood, but the inhibitors of PTK ( genistein, 2.6-26 micromol/L and tyrphostin, 0.5-5 micromol/L ) had no obvious effect on MCP-1 and IL-8 production ... Inhibitors of PKC ( calphostin C, 50-500 nmol/L and RO-31-8220, 10-100 nmol/L ), CaM ( W7, 28-280 micromol/L ), ERK1/2 MAPK ( PD 98059, 2-20 micromol/L ), p38 MAPK ( SB 203580, 0.6-6 micromol/L ), JNK MAPK ( curcumin, 2-10 micromol/L ), and NF-kappaB ( PDTC, 10-100 nmol/L ) markedly reduced Hcy 100 micromol/L induced production of MCP-1 and IL-8 in human cultured whole blood, but the inhibitors of PTK ( genistein, 2.6-26 micromol/L and tyrphostin, 0.5-5 micromol/L ) had no obvious effect on MCP-1 and IL-8 production
Sgarbanti et al., Ann N Y Acad Sci 2004 : These results suggest that in early phases of HIV-1 infection, before detectable cytokine production, NF-kappaB seems responsible for HIV-1 induced IRF-1 expression
Lee et al., Ann N Y Acad Sci 2004 : We also found that CAPE significantly inhibited H-ras induced NF-kappaB DNA binding activity without affecting the activation of extracellular signal regulated kinase and p38 mitogen activated protein kinase, which are major intracellular molecules involved in the Ras signaling pathways
Lee et al., Ann N Y Acad Sci 2004 : p7F also suppressed the serum level of TNF-alpha in mice treated with collagen and inhibited nuclear factor-kappaB (NF-kappaB) activation as well as NF-kappaB promoter activity in RAW 264.7 cells stimulated with LPS
Sitcheran et al., EMBO J 2005 : Positive and negative regulation of EAAT2 by NF-kappaB : a role for N-myc in TNFalpha controlled repression ... Additionally, the regulation of NF-kappaB by EGF is poorly understood ... Herein, we demonstrate that both TNFalpha mediated repression and EGF mediated activation of EAAT2 expression require NF-kappaB ... Herein, we demonstrate that both TNFalpha mediated repression and EGF mediated activation of EAAT2 expression require NF-kappaB ... We show that EGF activates NF-kappaB independently of signaling to IkappaB
Ku et al., Immunol Rev 2005 (Ectodermal Dysplasia...) : In these subjects, the NF-kappaB- and mitogen activated protein kinase mediated induction of inflammatory cytokines in response to TIR agonists is impaired
Yan et al., Immunol Cell Biol 2005 : VLP induced induction of costimulatory molecule expression, RelB activation and cytokine secretion by DC was blocked by inhibition of NF-kappaB activation, heparin or TLR4 mAb ... VLP induced induction of costimulatory molecule expression, RelB activation and cytokine secretion by DC was blocked by inhibition of NF-kappaB activation, heparin or TLR4 mAb
Akita et al., Virology 2005 : p21WAF1 modulates NF-kappaB signaling and induces anti-apoptotic protein Bcl-2 in Tax expressing rat fibroblast ... Previously, we reported that p21WAF1 stimulated Tax dependent NF-kappaB activation which influences a variety of cellular processes, including proliferation, differentiation, and apoptosis ... Previously, we reported that p21WAF1 stimulated Tax dependent NF-kappaB activation which influences a variety of cellular processes, including proliferation, differentiation, and apoptosis ... In HTLV-1 infected cells, Tax is primarily involved in the constitutive activation of NF-kappaB signaling ... Here, we demonstrate that p21WAF1 affects Tax dependent NF-kappaB signaling by inducing p100/52, an NF-kappaB related protein ... Here, we demonstrate that p21WAF1 affects Tax dependent NF-kappaB signaling by inducing p100/52, an NF-kappaB related protein
Harii et al., Mol Endocrinol 2005 (Thyroiditis, Autoimmune...) : In this report, we demonstrate TLR3 mRNA and protein are expressed on Fisher rat thyroid cell line-5 ( FRTL-5 ) thyroid cells and are functional because incubating cells with polyinosine-polycytidylic acid causes 1 ) transcriptional activation of both the nuclear factor kappaB (NF-kappaB)/Elk1 and interferon (IFN) regulatory factor-3/IFN-beta signal paths, 2 ) posttranscriptional activation of NF-kappaB and ERK1/2 , and 3 ) increased IFN-beta mRNA ... In this report, we demonstrate TLR3 mRNA and protein are expressed on Fisher rat thyroid cell line-5 ( FRTL-5 ) thyroid cells and are functional because incubating cells with polyinosine-polycytidylic acid causes 1 ) transcriptional activation of both the nuclear factor kappaB (NF-kappaB)/Elk1 and interferon (IFN) regulatory factor-3/IFN-beta signal paths, 2 ) posttranscriptional activation of NF-kappaB and ERK1/2, and 3 ) increased IFN-beta mRNA ... In this report, we demonstrate TLR3 mRNA and protein are expressed on Fisher rat thyroid cell line-5 ( FRTL-5 ) thyroid cells and are functional because incubating cells with polyinosine-polycytidylic acid causes 1 ) transcriptional activation of both the nuclear factor kappaB (NF-kappaB)/Elk1 and interferon (IFN) regulatory factor-3/IFN-beta signal paths, 2 ) posttranscriptional activation of NF-kappaB and ERK1/2, and 3 ) increased IFN-beta mRNA ... In this report, we demonstrate TLR3 mRNA and protein are expressed on Fisher rat thyroid cell line-5 ( FRTL-5 ) thyroid cells and are functional because incubating cells with polyinosine-polycytidylic acid causes 1 ) transcriptional activation of both the nuclear factor kappaB (NF-kappaB)/Elk1 and interferon (IFN) regulatory factor-3/IFN-beta signal paths, 2 ) posttranscriptional activation of NF-kappaB and ERK1/2, and 3 ) increased IFN-beta mRNA
Cataisson et al., J Immunol 2005 (Inflammation) : To determine whether TNFalpha mediated NF-kappaB translocation and subsequent expression of proinflammatory factors, K5-PKCalpha mice were treated systemically with a dimeric soluble form of p75 TNFR ( etanercept ) or crossed with mice deficient for both TNFR isoforms, and keratinocytes were cultured in the presence of TNFalpha neutralizing Abs
Li et al., J Mol Med (Berl) 2005 : LPS induced, TLR4 mediated signaling employs multiple TIR-domain containing adaptors, MyD88/TIRAP to mediate NFkappaB activation, TRIF/TRAM for IRF3 activation ... LPS induced, TLR4 mediated signaling employs multiple TIR-domain containing adaptors, MyD88/TIRAP to mediate NFkappaB activation, TRIF/TRAM for IRF3 activation
Blázovics et al., Orv Hetil 2004 (Inflammatory Bowel Diseases) : It became known, that oxidized LDL can inhibit LPS induced binding of the NF-kappaB to DNA and the subsequent expression of TNF-alpha and interleukin-1beta in macrophages as well as oxidized LDL modulates activation of NF-kappaB in mononuclear phagocytes by altering the degradation of I-kappaBs ... It became known, that oxidized LDL can inhibit LPS induced binding of the NF-kappaB to DNA and the subsequent expression of TNF-alpha and interleukin-1beta in macrophages as well as oxidized LDL modulates activation of NF-kappaB in mononuclear phagocytes by altering the degradation of I-kappaBs ... It became known, that oxidized LDL can inhibit LPS induced binding of the NF-kappaB to DNA and the subsequent expression of TNF-alpha and interleukin-1beta in macrophages as well as oxidized LDL modulates activation of NF-kappaB in mononuclear phagocytes by altering the degradation of I-kappaBs ... It became known, that oxidized LDL can inhibit LPS induced binding of the NF-kappaB to DNA and the subsequent expression of TNF-alpha and interleukin-1beta in macrophages as well as oxidized LDL modulates activation of NF-kappaB in mononuclear phagocytes by altering the degradation of I-kappaBs
Peppel et al., Cardiovasc Res 2005 (Graft Occlusion, Vascular...) : However, only TNF activated NF kappa B
Ahn et al., Cell Physiol Biochem 2005 : Wortmannin, LY294002 and dominant negative Akt, as well as dominant negative NF-kappaB inducing kinase (NIK) inhibited MUC2 reporter transcription, indicating that both phosphatidylinositol-3-OH kinase (PI3K)/Akt signaling pathway and NIK pathways mediate the effects of TNF-alpha ... Wortmannin inhibited NF-kappaB binding and transcriptional activity without inhibiting NF-kappaB translocation to the nucleus, indicating that PI3K/Akt signaling activates NF-kappaB transcriptional activity directly ... Wortmannin inhibited NF-kappaB binding and transcriptional activity without inhibiting NF-kappaB translocation to the nucleus, indicating that PI3K/Akt signaling activates NF-kappaB transcriptional activity directly ... However, the existence of this inhibitory JNK pathways suggests a mechanism whereby -- in the absence of NF-kappaB activation -- TNF-alpha production during inflammation in vivo could actually inhibit MUC2 production, giving rise to the defective mucosal protection which characterizes inflammatory bowel disease ... However, the existence of this inhibitory JNK pathways suggests a mechanism whereby -- in the absence of NF-kappaB activation -- TNF-alpha production during inflammation in vivo could actually inhibit MUC2 production, giving rise to the defective mucosal protection which characterizes inflammatory bowel disease
Shah et al., Cell Physiol Biochem 2005 (Calcium Signaling) : The antidiabetic agent rosiglitazone upregulates SERCA2 and enhances TNF-alpha- and LPS induced NF-kappaB dependent transcription and TNF-alpha induced IL-6 secretion in ventricular myocytes ... The antidiabetic agent rosiglitazone upregulates SERCA2 and enhances TNF-alpha- and LPS induced NF-kappaB dependent transcription and TNF-alpha induced IL-6 secretion in ventricular myocytes ... Since NF-kappaB dependent pathways, including the upregulation of IL-6 secretion, were shown to protect neonatal rat ventricular myocytes from apoptosis upon TNFalpha stimulation, additional experiments were designed to determine whether rosiglitazone enhances TNFalpha induced NF-kappaB dependent transcription and IL-6 secretion ... Because the endotoxin stress response in ventricular myocytes involves the upregulation of TNFalpha , and the activation of NF-kappaB , the effects of rosiglitazone on lipopolysaccharide induced NF-kappaB dependent transcription were also investigated ... Rosiglitazone had no significant effects on basal levels of NF-kappaB dependent transcription and IL-6 secretion
Hagi-Pavli et al., Cell Physiol Biochem 2005 : ICAM-1 was up regulated by both peptides and this was attenuated by the adenylyl cyclase inhibitor SQ22,536 and the NF-kappaB inhibitor SN-50
Yamaji et al., Thromb Res 2005 : APC in the range of 10-50 microg/mL could reduce lipopolysaccharide (LPS) induced ROS generation, nuclear factor kappaB (NF-kappaB) activation and resultant proinflammatory cytokine production
Chan et al., Biochem Biophys Res Commun 2005 : Tpl2/COT1 was shown to participate in CD40 signaling based on the ability of a catalytically inactive mutant to suppress CD40 mediated IkappaB kinase activation and induction of NFkappaB-responsive promoters, without affecting signaling by TNF ... Tpl2/COT1 was shown to participate in CD40 signaling based on the ability of a catalytically inactive mutant to suppress CD40 mediated IkappaB kinase activation and induction of NFkappaB-responsive promoters, without affecting signaling by TNF
Kokura et al., Cancer Lett 2005 (Adenocarcinoma...) : Recent papers indicate that NF-kappa B activation is inhibited by interleukin (IL)-10 ... Pretreating Colon 26 cells with IL-10 significantly attenuated the TNF-alpha induced NF-kappa B activation
Trauzold et al., FASEB J 2005 (Adenocarcinoma...) : Stimulation of TRAF2 overexpressing cells with CD95 ligand led to induction of NF-kappaB and AP-1, enhanced IL-8- and uPA-secretion, and a further increased invasiveness
Stangl et al., J Nutr 2005 : Phloretin did not affect TNF-alpha stimulated activation of nuclear factor kappaB (NF-kappaB) but inhibited activation of interferon regulatory factor 1 , a transcription factor involved in the regulation of endothelial cell adhesion molecule expression ... Phloretin did not affect TNF-alpha stimulated activation of nuclear factor kappaB (NF-kappaB) but inhibited activation of interferon regulatory factor 1, a transcription factor involved in the regulation of endothelial cell adhesion molecule expression
Glauert et al., J Nutr 2005 (Liver Neoplasms) : PCB-77 , but not PCB-153, increased hepatic NF-kappaB activity
Lam et al., Clin Cancer Res 2005 (Lymphoma, B-Cell...) : In the presence of tamoxifen, p65-ERD reversed the toxicity of IKK inhibition and restored expression of many NF-kappaB target genes
Gruden et al., J Am Soc Nephrol 2005 : Stretch activated the IkappaB-NF-kappaB pathway, and NF-kappaB inhibition, with the use of the specific inhibitor SN50, completely abolished stretch induced MCP-1, indicating that stretch induced MCP-1 was NF-kappaB dependent
Hidalgo et al., Br J Pharmacol 2005 : 2. In the present study, we analyzed the effect of andrographolide on the activation of NF-kappaB induced by platelet activating factor (PAF) and N-formyl-methionyl-leucyl-phenylalanine ( fMLP ) in HL-60 cells differentiated to neutrophils ... 4. Andrographolide ( 5 and 50 microM ) inhibited the NF-kappaB-luciferase activity induced by PAF
Lee et al., Prostate 2005 (Prostatic Neoplasms) : Requirement for NF-(kappa)B in interleukin-4 induced androgen receptor activation in prostate cancer cells ... The induction of AR activation by IL-4 can be suppressed by expression of the I(kappa)B(alpha) , an inhibitor of NF-(kappa)B ... IL-4 increases NF-(kappa)B transcriptional activity in prostate cancer cells which can be blocked by the addition of IL-4 antibody ... IL-4 can also rapidly activate NF-kappaB ... Furthermore, the IL-4 induced NF-kappaB activation and nuclear translocation can be blocked by LY294002, a PI3K/Akt specific inhibitor, suggesting that IL-4 induced NF-(kappa)B activation is mediated by activation of PI3K/Akt pathway
Ye et al., J Pathol 2005 (Lymphoma, B-Cell, Marginal Zone...) : The oncogenic activity of the three chromosomal translocations is linked by the physiological role of BCL10 and MALT1 in antigen receptor mediated NFkappaB activation ... The oncogenic activity of the three chromosomal translocations is linked by the physiological role of BCL10 and MALT1 in antigen receptor mediated NFkappaB activation
Jana et al., Free Radic Biol Med 2005 : All three cytokines alone induced the activation of AP-1 while IL-1beta and TNF-alpha but not IFN-gamma induced the activation of NF-kappaB ... All three cytokines alone induced the activation of AP-1 while IL-1beta and TNF-alpha but not IFN-gamma induced the activation of NF-kappaB ... However, the activation of NF-kappaB and C/EBPbeta was involved in the induction of iNOS by IL-1beta as well as by IL-IF
Jang et al., Cell Signal 2005 : Interestingly, there was PI3K dependent activation of AKT, p70S6K, JNKs, and NF-kappaB in response to catalase ... Interestingly, there was PI3K dependent activation of AKT, p70S6K, JNKs, and NF-kappaB in response to catalase
Yune et al., J Neurotrauma 2004 (Spinal Cord Injuries) : Manganese superoxide dismutase induced by TNF-beta is regulated transcriptionally by NF-kappaB after spinal cord injury in rats ... These results suggest that TNF-alpha may be an inducer of NF-kappaB activation and MnSOD expression after SCI and that MnSOD expression induced by TNF-alpha is likely mediated through activation of NF-kappaB
Kiss et al., J Neurochem 2005 : IL-1 activated NF-kappaB complexes consisted of p65 and p50, with p65 transiently phosphorylated on serine 536 in glial cells whereas more persistently in hepatic cells
Yagyu et al., J Clin Endocrinol Metab 2005 (Endometriosis) : The current study showed for the first time that Thal treatment attenuated the expression of IL-8 by reducing TNF-alpha induced NF-kappaB activation ... Here, we show 1 ) that treatment of endometriotic stromal cells with TNF-alpha increased the expression of phosphorylated IkappaBalpha and degradation of total IkappaBalpha, which in turn activates NF-kappaB ; 2 ) Thal significantly inhibits the TNF-alpha induced expression of phosphorylated IkappaBalpha and degradation of IkappaBalpha ; 3 ) TNF-alpha activation induced increased nuclear translocation of NF-kappaB , which was inhibited by pretreatment with either Thal or N-tosyl-L-phenylalanine chloromethyl ketone, an NF-kappaB inhibitor
Koyanagi et al., J Biol Chem 2005 : Reduction of the protein level of endogenous CPAP by RNA interference resulted in decreased activation of NF-kappaB by TNFalpha
Fan et al., Oncogene 2005 : The ability of HGF/SF to activate NF-kappaB signaling was dependent on c-Akt -- > Pak1 ( p21 associated kinase-1 ) signaling ( with Pak1 downstream of c-Akt ) and was inhibited by the tumor suppressor PTEN ( phosphatase and tensin homolog ) ... Inhibitors of phosphatidylinositol-3'-kinase and Src family kinases significantly inhibited HGF/SF mediated activation of NF-kappaB , while inhibitors of MEK, protein kinase C, and p70 S6 kinase had a modest effect or no effect on NF-kappaB activity ... HGF/SF induced the expression of several known NF-kappaB target genes ( cIAP-1 ( cellular inhibitor of apoptosis-1 ), cIAP-2, and TRAF-2 ( TNF receptor associated factor-2 ) ) in an NF-kappaB dependent manner ; HGF/SF blocked the inhibition of expression of these genes by ADR ... HGF/SF induced the expression of several known NF-kappaB target genes ( cIAP-1 ( cellular inhibitor of apoptosis-1 ), cIAP-2, and TRAF-2 ( TNF receptor associated factor-2 ) ) in an NF-kappaB dependent manner ; HGF/SF blocked the inhibition of expression of these genes by ADR ... HGF/SF induced the expression of several known NF-kappaB target genes ( cIAP-1 ( cellular inhibitor of apoptosis-1 ), cIAP-2, and TRAF-2 ( TNF receptor associated factor-2 ) ) in an NF-kappaB dependent manner ; HGF/SF blocked the inhibition of expression of these genes by ADR ... HGF/SF induced the expression of several known NF-kappaB target genes ( cIAP-1 ( cellular inhibitor of apoptosis-1 ), cIAP-2 , and TRAF-2 ( TNF receptor associated factor-2 ) ) in an NF-kappaB dependent manner ; HGF/SF blocked the inhibition of expression of these genes by ADR
Pozo-Guisado et al., Int J Cancer 2005 : NF-kappaB , a regulator of Bcl-2 expression, and calpain protease activity, a regulator of NF-kappaB, were both inhibited by RES
Lu et al., J Biol Chem 2005 : Whereas macrophages from Rip2-deficient mice showed impaired NF-kappaB and p38 mitogen activated protein kinase activation and reduced cytokine production in response to LPS stimulation, LPS signaling was intact in macrophages from mice that express Rip2 kinase-dead mutant
Moriuchi et al., Cancer Gene Ther 2005 (Brain Neoplasms...) : Following infection with TOIkappaB, nuclear translocation of NF-kappaB in U-87MG cells was significantly inhibited and caspase-3 activity increased compared with TOZ.1 infected cells
Nishitani et al., Biochem Biophys Res Commun 2005 : The cells that had been cotransfected with TLR4 ( Delta E24-P34 ) and MD-2 did not induce NF-kappa B activation in response to LPS
Savickiene et al., Int J Biochem Cell Biol 2005 (Leukemia) : p21 ( Waf1/Cip1 ) and FasL gene activation via Sp1 and NFkappaB is required for leukemia cell survival but not for cell death induced by diverse stimuli ... p21 ( Waf1/Cip1 ) and FasL gene activation via Sp1 and NFkappaB is required for leukemia cell survival but not for cell death induced by diverse stimuli
Migita et al., Rheumatology (Oxford) 2005 : A77 1726 inhibited IL-1beta induced p38 and c-Jun N-terminal kinase 1/2 ( JNK1/2 ) activation, whereas A77 1726 did not affect IL-1beta induced NF-kappaB activation in hepatocytes
Zhang et al., Cancer Res 2005 (Breast Neoplasms) : Serum induced SGK or increased expression of SGK activated NF-kappaB transcriptional activity, whereas small interference RNA to SGK blocked NF-kappaB activity ... Coexpression of SGK and IKKbeta significantly increased the activation of NF-kappaB ( versus expression of IKKbeta alone ) ... Coexpression of SGK and IKKbeta significantly increased the activation of NF-kappaB ( versus expression of IKKbeta alone ) ... Expression of dominant negative IKKbeta K44A, IkappaBalpha AA, and kinase-dead SGK ( 127KM ) blocked the ability of SGK to stimulate NF-kappaB activity, suggesting that IKKbeta is a target of SGK
Massa et al., J Biol Chem 2005 : Nevertheless, IKKalpha-/- mouse embryonic fibroblasts ( MEFs ) fail to express NF-kappaB targets in response to proinflammatory stimuli, uncovering a nuclear role for IKKalpha in NF-kappaB activation ... However, it remains unknown whether the global defect in NF-kappaB dependent gene expression of IKKalpha-/- cells is caused by the absence of IKKalpha kinase activity
Li et al., Kidney Int 2005 (Inflammation) : Both EPA and DHA at 10 micromol/L and 100 micromol/L concentrations effectively decreased lipopolysaccharide (LPS) induced nuclear factor-kappaB (NF-kappaB) activation and monocyte chemoattractant protein-1 ( MCP-1 ) expression ... A dose of 100 micromol/L bisphenol A diglycidyl ether ( BADGE ) abolished the PPAR-gamma activation induced by both EPA and DHA and removed the inhibitory effect of EPA and DHA on LPS induced NF-kappaB activation in HK-2 cells ... Overexpression of PPAR-gamma further inhibited NF-kappaB activation compared to the control cells in the presence of EPA and DHA ... Our data demonstrate that both EPA and DHA down-regulate LPS induced activation of NF-kappaB via a PPAR-gamma dependent pathway in HK-2 cells
Zhu et al., J Steroid Biochem Mol Biol 2004 (MAP Kinase Signaling System) : Further studies showed decreased p27 expression, increased PKC-alpha expression and enhanced basal and LPS induced NF-kappaB activity in RAW- ( GR- ) cells as compared to the RAW-control cells ... This effect may be associated with the decreased expression of p27 , the increased expression of PKC-alpha, and the activation of NF-kappaB ... This effect may be associated with the decreased expression of p27, the increased expression of PKC-alpha , and the activation of NF-kappaB
Wang et al., Clin Chim Acta 2005 : However, the effects of statin on the expression of TNF-alpha and activation of NF-kappaB in endothelial cells stimulated by CRP are less studied ... However, the effects of statin on the expression of TNF-alpha and activation of NF-kappaB in endothelial cells stimulated by CRP are less studied ... The CRP also significantly induces the activation of NF-kappaB in endothelial cells, and those effects were apparently inhibited by 10 micromol/l of fluvastatin, but not complete ... CRP stimulation result in induction of TNF-alpha and activation of NF-kappaB , and this effect could be significantly inhibited by fluvastatin, suggesting that CRP may play a direct role in atherogenesis by activating endothelial cells, and statins inhibit this response, which may provide an insight into the mechanisms of anti-inflammatory or anti-atherosclerotic actions of statins
Li et al., Di Yi Jun Yi Da Xue Xue Bao 2005 (Burns) : PDTC could effectively inhibit endothelial IkappaBalpha degradation and activation of NF-kappaB induced by the burn serum
Shinohara et al., J Exp Med 2005 : The stimulation with LPS of macrophages from mice lacking Dok-1 or Dok-2 induced elevated Erk activation, but not the other MAP kinases or NF-kappaB , resulting in hyperproduction of TNF-alpha and nitric oxide
Kimura et al., J Exp Med 2005 : Shn-2-deficient developing Th2 cells showed constitutive activation of nuclear factor kappaB (NF-kappaB) and enhanced GATA3 induction
Na et al., J Pharmacol Sci 2005 (Dermatitis, Atopic) : In addition, SH inhibited the increase of TNF-alpha induced NF-kappaB protein levels, transcription factor of TNF-alpha from 293T cells
Hasegawa et al., J Biol Chem 2005 (Inflammation) : ASC mediated NF-kappaB activation leading to interleukin-8 production requires caspase-8 and is inhibited by CLARP ... ASC mediated NF-kappaB activation leading to interleukin-8 production requires caspase-8 and is inhibited by CLARP ... The catalytic activity of caspase-8 was required for the ASC mediated NF-kappaB activation when caspase-8 was expressed at an endogenous level, although it was not essential when caspase-8 was overexpressed ... The catalytic activity of caspase-8 was required for the ASC mediated NF-kappaB activation when caspase-8 was expressed at an endogenous level, although it was not essential when caspase-8 was overexpressed ... These results are the first to indicate that caspase-8 plays an important role in the ASC mediated NF-kappaB activation, and that the ASC mediated NF-kappaB activation actually induces physiologically relevant gene expression
Kunsch et al., J Pharmacol Exp Ther 2005 : AGIX-4207 did not inhibit TNF-alpha induced nuclear translocation of nuclear factor of the kappa-enhancer in B cells ( NF-kappaB ), suggesting that the mechanism of action is independent of this redox-sensitive transcription factor
Debidda et al., J Biol Chem 2005 (Inflammation) : STAT3 was required for the RhoA induced NF-kappaB and cyclin D1 transcription and was involved in NF-kappaB nuclear translocation ... STAT3 was required for the RhoA induced NF-kappaB and cyclin D1 transcription and was involved in NF-kappaB nuclear translocation
Li et al., Cancer Res 2005 (Fibrosarcoma) : Furthermore, a specific NF-kappaB inhibitor significantly suppressed IL-12/IL-18 induced IFNgamma secretion, thus confirming the requirement for NF-kappaB activation in IL-12/IL-18 signaling
Harhaj et al., Virology 2005 : CD40 ligation strongly synergized with Tax to activate NF-kappaB, suggesting that CD40 signals may costimulate Tax mediated NF-kappaB activation, particularly when Tax is expressed at low levels
Hauer et al., Proc Natl Acad Sci U S A 2005 : In an attempt to delineate the role of individual TRAFs, we compared NF-kappaB activation by CD40 ( wt ) and CD40 mutants with different TRAF recruitment patterns ... We observed that TRAF3 specifically blocked the NF-kappaB activation via TRAF2/5 ... Paradoxically, suppression of TRAF2/5 mediated NF-kappaB activation by TRAF3 resulted in enhanced transcriptional activity of TRAF6 mediated canonical NF-kappaB emanating from CD40 ... Paradoxically, suppression of TRAF2/5 mediated NF-kappaB activation by TRAF3 resulted in enhanced transcriptional activity of TRAF6 mediated canonical NF-kappaB emanating from CD40
Matsumoto et al., Clin Cancer Res 2005 (Breast Neoplasms) : In this cell line, DHMEQ completely inhibited the tumor necrosis factor-alpha induced activation of NF-kappaB
Nishiyama et al., Int Immunopharmacol 2005 : Cyclosporin A inhibits the early phase of NF-kappaB/RelA activation induced by CD28 costimulatory signaling to reduce the IL-2 expression in human peripheral T cells ... Antisense NF-kappaB p65/RelA oligonucleotides inhibited p65/RelA mRNA expression, diminished IL-2 mRNA expression in the costimulated HPTC and reduced HPTC proliferation to the same extent as CsA added simultaneously with the costimulation
Lee et al., Biochem Pharmacol 2005 : The results show that HCA, among the derivatives, most significantly inhibited lipopolysaccharide (LPS) induced NO production and NF-kappa B transcriptional activity in a dose dependent manner with an IC ( 50 ) value of 8 and 22 microM, respectively ... Moreover, HCA inhibited LPS induced p50 and p65 translocation resulting in the inhibition of the DNA binding activity of the NF-kappa B , a central regulator of iNOS
Muller et al., Nat Med 2005 (Cell Transformation, Neoplastic...) : Mouse knockout studies indicate that Bin1 loss elevates the STAT1- and NF-kappaB dependent expression of IDO , driving escape of oncogenically transformed cells from T cell dependent antitumor immunity
Hosokawa et al., Apoptosis 2005 (Lymphoma, B-Cell, Marginal Zone...) : This stability of API2-MALT1 may thus result in inappropriate nuclear factor (NF)-kappaB activation, thereby contributing to the pathogenesis of MALT lymphoma ... This stability of API2-MALT1 may thus result in inappropriate nuclear factor (NF)-kappaB activation, thereby contributing to the pathogenesis of MALT lymphoma ... Recent biochemical and genetic studies have clearly shown that BCL10 and MALT1 form a physical and functional complex and are both required for NF-kappaB activation by antigen receptor stimulation in T and B lymphocytes ... Recent biochemical and genetic studies have clearly shown that BCL10 and MALT1 form a physical and functional complex and are both required for NF-kappaB activation by antigen receptor stimulation in T and B lymphocytes ... It has also been shown that CARMA1 , a newly discovered member of the membrane associated guanylate kinase ( MAGUK ) families, is critical for antigen receptor stimulated NF-kappaB activation ... Furthermore, BCL10/MALT1- and API2-MALT1 induced NF-kappaB activation may contribute to anti-apoptotic action probably through NF-kappaB mediated upregulation of apoptotic inhibitor genes ... Furthermore, BCL10/MALT1- and API2-MALT1 induced NF-kappaB activation may contribute to anti-apoptotic action probably through NF-kappaB mediated upregulation of apoptotic inhibitor genes
Ko et al., Toxicol In Vitro 2005 : We also demonstrated that RVS glycoprotein inhibits activities of the nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) induced by G/GO, and prevents from G/GO induced apoptosis in the NIH/3T3 cells
Fernyhough et al., J Neurosci 2005 (MAP Kinase Signaling System...) : ELISA revealed that these cultures contained TNFalpha , and exposure to an anti-TNFalpha antibody inhibited NF-kappaB DNA binding activity by approximately 35 % and killed approximately 40 % of medium to large neurons within 24 h
Chen et al., Carcinogenesis 2005 : Furthermore, impairing nuclear factor-kappaB (NFkappaB) activation by either NEMO-BDBP, an NFkappaB specific inhibitor, or overexpression of DN-IkappaBalpha or IKK-KM markedly inhibited the expression of B [ a ] P-induced iNOS, suggesting that the NFkappaB pathway is also required for the induction of iNOS by B [ a ] P ... Furthermore, impairing nuclear factor-kappaB (NFkappaB) activation by either NEMO-BDBP, an NFkappaB specific inhibitor, or overexpression of DN-IkappaBalpha or IKK-KM markedly inhibited the expression of B [ a ] P-induced iNOS, suggesting that the NFkappaB pathway is also required for the induction of iNOS by B [ a ] P ... Furthermore, impairing nuclear factor-kappaB (NFkappaB) activation by either NEMO-BDBP, an NFkappaB specific inhibitor, or overexpression of DN-IkappaBalpha or IKK-KM markedly inhibited the expression of B [ a ] P-induced iNOS , suggesting that the NFkappaB pathway is also required for the induction of iNOS by B [ a ] P ... Furthermore, impairing nuclear factor-kappaB (NFkappaB) activation by either NEMO-BDBP , an NFkappaB specific inhibitor, or overexpression of DN-IkappaBalpha or IKK-KM markedly inhibited the expression of B [ a ] P-induced iNOS, suggesting that the NFkappaB pathway is also required for the induction of iNOS by B [ a ] P
Sola et al., Pediatr Res 2005 (Brain Ischemia...) : The most effective dose after FCI was 1000 U/kg for 3 d. Immunoanalyses showed that Epo induced a significant increase in phosphorylated Janus kinase 2 and signal transducer and activator of transcription-5 expressions at 1 and 3 d and up-regulated Bcl-xL expression by 24 h after FCI but did not affect Epo receptor or NF-kappaB expression
Chiu et al., Arterioscler Thromb Vasc Biol 2005 (Vasculitis) : Shear stress inhibited the SMC coculture induced NF-kappaB activation in ECs and monocytic THP-1 cell adhesion to ECs
García-Bueno et al., Psychopharmacologia 2005 : The mechanisms involved in the anti-oxidative properties of 15d-PGJ2 in stress involve NFkappaB blockade ( by preventing stress induced IkappaBalpha decrease ) as well as inhibition of TNFalpha release in stressed animals
Rahim et al., Immunology 2005 : In a complementary experiment, in vitro transient expression of p65 NF-kappaB could not rescue the inhibitory effect of IL-10 on IL-12 production, suggesting that NF-kappaB alone was not sufficient to restore IL-12 production during IL-10 treatment
Higashihara et al., Redox Rep 2004 : These results suggest that NF-kappaB and PPAR-gamma regulate H/R stimulated IL-8 production in U937 cells
Planavila et al., Cardiovasc Res 2005 (Cardiomegaly) : Electrophoretic mobility shift assay ( EMSA ) revealed that L-165041 significantly decreased LPS stimulated NF-kappaB binding activity in H9c2 myotubes ... Finally, coimmunoprecipitation studies showed that L-165041 strongly enhanced the physical interaction between PPARbeta/delta and the p65 subunit of NF-kappaB, suggesting that increased association between these two proteins is the mechanism responsible for antagonizing NF-kappaB activation by PPARbeta/delta activators ... These results suggest that PPARbeta/delta activation inhibits PE-induced cardiac hypertrophy and LPS induced NF-kappaB activation ... These results suggest that PPARbeta/delta activation inhibits PE-induced cardiac hypertrophy and LPS induced NF-kappaB activation
Kang et al., Cell Signal 2005 (Autoimmune Diseases) : IL-12 production is differentially regulated through multiple pathways, which can be classified as follows : nuclear factor-kappaB (NF-kappaB) and other transcription factors, p38 mitogen activated protein ( MAP ) kinase, cyclic adenosine monophosphate ( cyclic AMP ) -modulating molecules, cell membrane ion channels and pumps, nitric oxide ( NO ), and receptors
Davies et al., Cell Signal 2005 (Carcinoma) : The activation of NF-kappaB and phosphatidylinositol-3 (PI3) kinase by TNF-alpha and TRAIL overrides the pro-apoptotic effects of these ligands in carcinoma cells and hinders their therapeutic application ... The activation of NF-kappaB and phosphatidylinositol-3 (PI3) kinase by TNF-alpha and TRAIL overrides the pro-apoptotic effects of these ligands in carcinoma cells and hinders their therapeutic application ... Moreover, no cross-talk between the two pathways was observed, as the specific suppression of PI3 kinase with chemical inhibitors did not influence CD40 mediated IkappaBalpha phosphorylation and degradation or NF-kappaB binding and transactivation
Korcok et al., J Biol Chem 2005 (Inflammation) : P2Y6 nucleotide receptors activate NF-kappaB and increase survival of osteoclasts
Catley et al., Br J Pharmacol 2005 (Asthma) : Overexpression of IKK beta ( KA ) or I kappa B alpha delta N prevented NF-kappa B-dependent transcription and DNA binding
Seki et al., Hepatology 2005 : In Myd88 ( -/- ) mice after PH, induction of expression of immediate early genes involved in hepatocyte replication and phosphorylation of STAT3 in the liver, and production of TNF-alpha/IL-6 by and activation of NF-kappaB in the Kupffer cells were grossly subnormal and were associated with impaired liver regeneration ... In Myd88 ( -/- ) mice after PH, induction of expression of immediate early genes involved in hepatocyte replication and phosphorylation of STAT3 in the liver, and production of TNF-alpha/IL-6 by and activation of NF-kappaB in the Kupffer cells were grossly subnormal and were associated with impaired liver regeneration ... However, TLR2 , 4 and 9, which recognize gram negative and -positive bacterial products, are not essential for NF-kappaB activation and IL-6 production after PH, which excludes a possible contribution of TLR2/TLR4 or TLR9 to MyD88 mediated pathways
Jijon et al., Eur J Immunol 2005 : Human HT-29 and Caco-2 monolayers were stimulated with recombinant SAA and NF-kappaB activation/NF-kappaB dependent gene expression measured ... SAA induced IkappaB-alpha degradation, RelA serine 536 ( S536 ) phosphorylation, NF-kappaB transcriptional activity, RelA recruitment to the IL-8 gene promoter and endogenous gene expression ( IL-8, COX-2 ) in HT-29 cells ... Further, Ad5IkappaBAA abrogated SAA induced RelA nuclear translocation, NF-kappaB transcriptional activity and IL-8 gene expression
Lee et al., Leuk Res 2005 : The DMSO induced upregulation of PTEN might be mediated by NF-kappaB activation, which was evidenced by the blockage of DMSO induced PTEN upregulation with an NF-kappaB inhibitor, pyrrolidine dithiocarbamate ( PDTC )
Petlickovski et al., Blood 2005 (Leukemia, Lymphocytic, Chronic, B-Cell...) : This response was characterized by transient phosphorylation of extracellular signal related kinase ( ERK ) and Akt ( protein kinase B [ PKB ] ), lack of activation of c-JUN NH2-terminal kinase (JNK) and p38 mitogen activated protein kinase ( MAPK ), and variable activation of phospholipase Cgamma2 ( PLCgamma2 ) and nuclear factor-kappaB (NF-kappaB) ... This response was characterized by transient phosphorylation of extracellular signal related kinase ( ERK ) and Akt ( protein kinase B [ PKB ] ), lack of activation of c-JUN NH2-terminal kinase (JNK) and p38 mitogen activated protein kinase ( MAPK ), and variable activation of phospholipase Cgamma2 ( PLCgamma2 ) and nuclear factor-kappaB (NF-kappaB) ... This response was characterized by transient phosphorylation of extracellular signal related kinase ( ERK ) and Akt ( protein kinase B [ PKB ] ), lack of activation of c-JUN NH2-terminal kinase (JNK) and p38 mitogen activated protein kinase ( MAPK ), and variable activation of phospholipase Cgamma2 ( PLCgamma2 ) and nuclear factor-kappaB (NF-kappaB) ... This response was characterized by transient phosphorylation of extracellular signal related kinase ( ERK ) and Akt ( protein kinase B [ PKB ] ), lack of activation of c-JUN NH2-terminal kinase (JNK) and p38 mitogen activated protein kinase ( MAPK ), and variable activation of phospholipase Cgamma2 ( PLCgamma2 ) and nuclear factor-kappaB (NF-kappaB)
Huang et al., J Immunol 2005 (Wiskott-Aldrich Syndrome) : The Wiskott-Aldrich syndrome protein regulates nuclear translocation of NFAT2 and NF-kappa B ( RelA ) independently of its role in filamentous actin polymerization and actin cytoskeletal rearrangement
Moreno-García et al., J Immunol 2005 : Loss of PKC activity blocked CD38 dependent, B cell proliferation, NF-kappaB activation , and subsequent expression of cyclin-D2
Rodriguez-Galán et al., J Immunol 2005 : The IL-2-plus-IL-18 or IL-12-plus-IL-18 effect on IFN-gamma and IL-13 expression is dependent on Stat4 and NF-kappaB but independent of Stat6, T-bet, or NFAT ... The IL-2-plus-IL-18 or IL-12-plus-IL-18 effect on IFN-gamma and IL-13 expression is dependent on Stat4 and NF-kappaB but independent of Stat6, T-bet, or NFAT
Vila-del Sol et al., J Immunol 2005 : Involvement of TNF and NF-kappa B in the transcriptional control of cyclooxygenase-2 expression by IFN-gamma in macrophages ... Taken together our results suggest a model in which IFN-gamma induced TNF activates NF-kappaB , which is required for full COX-2 expression
Preciado et al., J Immunol 2005 : As one potential mechanism for this increase in proliferation, an NF-kappaB-specific activation of cyclin D1 was observed
Ohishi et al., J Immunol 2005 (Bone Resorption) : In SOCS3-deficient BMMs, expression levels of TNF-receptor associated factor-6 and IkappaB were drastically reduced and receptor activator of the NF-kappaB ligand induced IkappaB phosphorylation was severely impaired in the presence of IL-6 ... In SOCS3-deficient BMMs, expression levels of TNF-receptor associated factor-6 and IkappaB were drastically reduced and receptor activator of the NF-kappaB ligand induced IkappaB phosphorylation was severely impaired in the presence of IL-6 ... These data suggest that both SOCS1 and SOCS3 regulate osteoclastogenesis by blocking the inhibitory effect of inflammatory cytokines on receptor activator of the NF-kappaB ligand mediated osteoclast differentiation signals ... These data suggest that both SOCS1 and SOCS3 regulate osteoclastogenesis by blocking the inhibitory effect of inflammatory cytokines on receptor activator of the NF-kappaB ligand mediated osteoclast differentiation signals
Planavila et al., J Biol Chem 2005 (Hypertrophy) : Lipopolysaccharide stimulation of NF-kappaB in embryonic rat heart derived H9c2 myotubes, which only express PPARbeta/delta, caused both a reduction in Pdk4 expression and DNA binding activity of PPARbeta/delta to its response element, effects that were reversed by NF-kappaB inhibitors ... Lipopolysaccharide stimulation of NF-kappaB in embryonic rat heart derived H9c2 myotubes, which only express PPARbeta/delta, caused both a reduction in Pdk4 expression and DNA binding activity of PPARbeta/delta to its response element, effects that were reversed by NF-kappaB inhibitors ... In hypertrophied hearts of banded rats the reduction in the expression of Pdk4 was accompanied by activation of NF-kappaB and enhanced interaction between p65 and PPARbeta/delta ... In hypertrophied hearts of banded rats the reduction in the expression of Pdk4 was accompanied by activation of NF-kappaB and enhanced interaction between p65 and PPARbeta/delta
Jeyaseelan et al., Infect Immun 2005 (Respiratory Distress Syndrome, Adult) : Our results show that CD14 and TLR4 are necessary for low-dose ( 300-microg/ml ) LPS induced microvascular leakage, NF-kappaB activation, neutrophil influx, cytokine and chemokine ( KC, macrophage inflammatory protein 2, tumor necrosis factor alpha, interleukin-6 ) expression, and subsequent lung damage ... Our results show that CD14 and TLR4 are necessary for low-dose ( 300-microg/ml ) LPS induced microvascular leakage, NF-kappaB activation, neutrophil influx, cytokine and chemokine ( KC, macrophage inflammatory protein 2, tumor necrosis factor alpha, interleukin-6 ) expression, and subsequent lung damage ... Our results show that CD14 and TLR4 are necessary for low-dose ( 300-microg/ml ) LPS induced microvascular leakage, NF-kappaB activation , neutrophil influx, cytokine and chemokine ( KC, macrophage inflammatory protein 2, tumor necrosis factor alpha, interleukin-6 ) expression, and subsequent lung damage
Benito et al., Ann Rheum Dis 2005 (Disease Progression...) : The inflammatory cell infiltrate, blood vessel formation, and angiogenic factors, NF-kappaB activation , expression of tumour necrosis factor alpha (TNFalpha) and interleukin 1beta (IL1beta), and the presence of cyclo-oxygenase (COX)-1 and COX-2 were quantified ... The inflammatory cell infiltrate, blood vessel formation, and angiogenic factors, NF-kappaB activation , expression of tumour necrosis factor alpha (TNFalpha) and interleukin 1beta (IL1beta), and the presence of cyclo-oxygenase (COX)-1 and COX-2 were quantified ... The inflammatory cell infiltrate, blood vessel formation, and angiogenic factors, NF-kappaB activation , expression of tumour necrosis factor alpha (TNFalpha) and interleukin 1beta (IL1beta) , and the presence of cyclo-oxygenase (COX)-1 and COX-2 were quantified ... The inflammatory cell infiltrate, blood vessel formation, and angiogenic factors, NF-kappaB activation , expression of tumour necrosis factor alpha (TNFalpha) and interleukin 1beta (IL1beta), and the presence of cyclo-oxygenase (COX)-1 and COX-2 were quantified ... The inflammatory cell infiltrate, blood vessel formation, and angiogenic factors, NF-kappaB activation , expression of tumour necrosis factor alpha (TNFalpha) and interleukin 1beta (IL1beta), and the presence of cyclo-oxygenase (COX)-1 and COX-2 were quantified
Andela et al., J Clin Pathol 2005 (Bone Neoplasms...) : Dominant negative inhibition of nuclear factor kappa B (NF kappa B) signalling activity in a human osteosarcoma cell line ( Saos2 ) results in malignant reversion and the induction of the osteoblast differentiating transcription factor , Runx2/Cbfa1
Park et al., Oncogene 2005 : ECop ( EGFR coamplified and overexpressed protein ), a novel protein, regulates NF-kappaB transcriptional activity and associated apoptotic response in an IkappaBalpha dependent manner ... ECop ( EGFR coamplified and overexpressed protein ), a novel protein, regulates NF-kappaB transcriptional activity and associated apoptotic response in an IkappaBalpha dependent manner ... Ectopic expression of ECop increases NF-kappaB transcriptional activity by promoting nuclear translocation and DNA binding of NF-kappaB, and ECop induced NF-kappaB activation confers cellular resistance to apoptotic challenge
Volanti et al., Oncogene 2005 (Neoplasms) : We report that PDT induces COX-2 expression in these cells and this expression is mainly due to nuclear factor kappa B (NF-kappaB) dependent transcription of cox-2 gene without any post-transcriptional regulation ... In T24, NF-kappaB activation occurs through a protein kinase C (PKC)alpha- and phosphoinositide-3-kinase (PI3K) dependent I kappa B kinase (IKK) complex activation, whereas in HeLa cells, NF-kappaB activation is mediated by PKC- and PI3K independent IKK complex activation
Kim et al., Oncogene 2005 (Skin Neoplasms) : The p38 MAP kinase inhibitor SB203580 attenuated NF-kappaB activation and subsequent COX-2 induction in TPA treated mouse skin
Kim et al., Biochem Biophys Res Commun 2005 : 8-Hydroxyquinoline inhibits iNOS expression and nitric oxide production by down regulating LPS induced activity of NF-kappaB and C/EBPbeta in Raw 264.7 cells ... 8-Hydroxyquinoline inhibits iNOS expression and nitric oxide production by down regulating LPS induced activity of NF-kappaB and C/EBPbeta in Raw 264.7 cells ... Taken together, these results suggest that 8HQ acts to inhibit inflammation through inhibition of NO production and iNOS expression through blockade of C/EBPbeta DNA binding activity and NF-kappaB activation
Elouil et al., Diabetologia 2005 (MAP Kinase Signaling System) : To test this hypothesis, we compared the effects of IL-1beta , high glucose, and hydrogen peroxide, on NFkappaB DNA binding activity and target gene mRNA levels in cultured rat islets ... IL-1beta consistently increased islet NFkappaB activity and c-Myc, haeme-oxygenase 1, inducible nitric oxide synthase (iNOS), Fas, and inhibitor of NFkappaB alpha ( IkappaBalpha ) mRNA levels
Yabe et al., Glia 2005 : Pigment epithelium derived factor induces pro-inflammatory genes in neonatal astrocytes through activation of NF-kappa B and CREB
Kim et al., Mol Cell Biol 2005 : However, LIGHT induced NF-kappaB and JNK activation can be restored by ectopic expression of TRAF2 in TRAF2-/- cells
Arnold et al., Mol Cell Biol 2005 : Upon T-cell receptor stimulation, PKD robustly augments HPK1 kinase activity in Jurkat T cells and enhances HPK1-driven SAPK/JNK and NF-kappaB activation ; conversely, antisense down-regulation of PKD results in reduced HPK1 activity
Kim et al., Mol Cell Biol 2005 : Here we report that eIF4GI, which is a scaffold protein interacting with many translation factors, interacts with TRAF2, a signaling molecule that plays a key role in activation of NF-kappaB through TNF-alpha
Gutierrez et al., Development 2005 : Inhibiting NF-kappaB activation with super-repressor IkappaB-alpha, BAY 11 7082 ( IkappaB-alpha phosphorylation inhibitor ) or N-acetyl-Leu-Leu-norleucinal ( proteosomal degradation inhibitor ), or inhibiting NF-kappaB transcriptional activity with kappaB decoy DNA substantially reduced the size and complexity of the neurite arbors of sensory neurons cultured with brain derived neurotrophic factor while having no effect on their survival
Su et al., Science 2005 (Immunologic Deficiency Syndromes) : Requirement for caspase-8 in NF-kappaB activation by antigen receptor
Ahn et al., Life Sci 2005 : Treatment with these compounds of RAW 264.7 cells transfected with a reporter construct indicated a reduced level of LPS induced nuclear factor-kappaB (NF-kappaB) activity and effectively lowered NF-kappaB binding as measured by electrophoretic mobility shift assay ( EMSA )
Ojeda et al., Inflamm Res 2005 : Also, DLE induced TLR2 and TLR4 expression, and increased cAMP levels, whereas NF-kappaB activity was inhibited ... Also, DLE induced TLR2 and TLR4 expression, and increased cAMP levels, whereas NF-kappaB activity was inhibited
Ng et al., Kidney Int Suppl 2005 (Nephritis) : Furthermore, gene transfer of Smad7 also substantially blocked nuclear factor kappa B (NFkappaB) activation in the rat remnant kidney ( P < 0.01 )
Yang et al., Biochem Biophys Res Commun 2005 : The two peptides were found to significantly inhibit LPS induced NF-kappaB activation in HEK-293 cells that were transfected with TLR4 cDNA, LPS induced IkappaBalpha ( IkappaB alpha ) phosphorylation and NF-kappaB activation in monocytes, and release of IL-1, IL-6, and TNF-alpha by monocytes
Barnich et al., J Biol Chem 2005 : GRIM-19 is required for NF-kappaB activation following NOD2 mediated recognition of bacterial muramyl dipeptide
Andresen et al., Gut 2005 (Colitis, Collagenous...) : As the transcription factor nuclear factor kappaB (NFkappaB) is a major inducer of iNOS gene expression, we compared activation and transcriptional activity of NFkappaB in colonic mucosal biopsies from these patients
Salanova et al., FASEB J 2005 (Fever) : Finally, we show that beta2-integrins, which are not necessary for TNF-alpha induced NF-kappaB activation at 37 degrees C, transduce costimulatory signals allowing NF-kappaB activation after heat exposure
Hu et al., Mol Cancer Res 2005 : Trichostatin A inhibits cyclin D1 expression in a NF-kappaB dependent manner in JB6 cells
Kriehuber et al., Blood 2005 : Ligation of tumor necrosis factor receptor superfamily ( TNFR-SF ) members on DCs and cognate contact with T cells resulted in quantitatively balanced nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK) mediated activator protein-1 (AP-1) induction and strongly enhanced DC longevity
Shim et al., J Biol Chem 2005 (Inflammation...) : Pretreatment with protein kinase C ( PKC ) inhibitor blocked the TPA induced increase in NAG-1 protein levels and NF-kappa B binding/transcriptional activity, whereas an inhibition of p38 , JNK, MEK activity had no effect on TPA induced NAG-1 levels and NF-kappa B transcriptional activity
Rangaswami et al., J Biol Chem 2005 (Neoplasm Invasiveness) : We have recently demonstrated that nuclear factor inducing kinase ( NIK ) plays a crucial role in osteopontin (OPN) induced mitogen activated protein kinase/I kappa B alpha kinase dependent nuclear factor kappa B (NF kappa B)-mediated promatrix metalloproteinase-9 activation ( Rangaswami, H., Bulbule, A., and Kundu, G. C. ( 2004 ) J. Biol. Chem. 279, 38921-38935 ) ... We have recently demonstrated that nuclear factor inducing kinase ( NIK ) plays a crucial role in osteopontin (OPN) induced mitogen activated protein kinase/I kappa B alpha kinase dependent nuclear factor kappa B (NF kappa B)-mediated promatrix metalloproteinase-9 activation ( Rangaswami, H., Bulbule, A., and Kundu, G. C. ( 2004 ) J. Biol. Chem. 279, 38921-38935 ) ... We have recently demonstrated that nuclear factor inducing kinase ( NIK ) plays a crucial role in osteopontin (OPN) induced mitogen activated protein kinase/I kappa B alpha kinase dependent nuclear factor kappa B (NF kappa B)-mediated promatrix metalloproteinase-9 activation ( Rangaswami, H., Bulbule, A., and Kundu, G. C. ( 2004 ) J. Biol. Chem. 279, 38921-38935 ) ... We have recently demonstrated that nuclear factor inducing kinase ( NIK ) plays a crucial role in osteopontin (OPN) induced mitogen activated protein kinase/I kappa B alpha kinase dependent nuclear factor kappa B (NF kappa B)-mediated promatrix metalloproteinase-9 activation ( Rangaswami, H., Bulbule, A., and Kundu, G. C. ( 2004 ) J. Biol. Chem. 279, 38921-38935 )
Nadjar et al., J Cereb Blood Flow Metab 2005 (Brain Neoplasms...) : NFkappaB activates in vivo the synthesis of inducible Cox-2 in the brain ... Incubation of rat glioma cells with the NBD peptide blocked IL-1beta induced NFkappaB nuclear translocation ... Treatment with NBD peptide completely abolished IL-1beta induced NFkappaB activation and Cox-2 synthesis in microvasculature ... These findings strongly support the hypothesis that IL-1beta induced NFkappaB activation plays a major role in transmission of immune signals from the periphery to the brain
Peng et al., Zhonghua Gan Zang Bing Za Zhi 2005 (Reperfusion Injury) : Glycine could efficiently protect rat liver grafts from ischemia-reperfusion injury by repressing the expression of CD14 and NF-kappa B binding activity in Kupffer cells and inhibiting the productions of TNF alpha and IL-1 ... Glycine could efficiently protect rat liver grafts from ischemia-reperfusion injury by repressing the expression of CD14 and NF-kappa B binding activity in Kupffer cells and inhibiting the productions of TNF alpha and IL-1
Xu et al., J Clin Invest 2005 (Arthritis, Rheumatoid...) : Furthermore, it was evident that OPN activated transcription factor NF-kappaB in mononuclear cells
Wu et al., Cell Signal 2005 : NGF stimulated phosphorylation of Bad and IKK and transcriptional activity of NFkappaB were indeed sensitive to treatments with PTX
Mortaz et al., Biochem Pharmacol 2005 : Concomitantly, ASA inhibited nuclear factor (NF)-kappaB activity, as well as the phosphorylation and breakdown of the inhibitory protein IkappaB-alpha ... We thus propose that the anti-inflammatory effects of ASA in mast cells are due to suppression of IkappaB kinase activity, thereby inhibiting subsequent phosphorylation and degradation of IkappaB-alpha, activation of NF-kappaB , and transcription of proinflammatory cytokines ... We thus propose that the anti-inflammatory effects of ASA in mast cells are due to suppression of IkappaB kinase activity, thereby inhibiting subsequent phosphorylation and degradation of IkappaB-alpha, activation of NF-kappaB , and transcription of proinflammatory cytokines ... We thus propose that the anti-inflammatory effects of ASA in mast cells are due to suppression of IkappaB kinase activity, thereby inhibiting subsequent phosphorylation and degradation of IkappaB-alpha , activation of NF-kappaB , and transcription of proinflammatory cytokines
Takatsuna et al., J Bone Miner Res 2005 (Bone Resorption) : RANKL induces activation of NF-kappaB and osteoclastogenesis in bone marrow derived monocyte/macrophage precursor cells ( BMMs ) ... ( - ) -DHMEQ strongly inhibited RANKL induced NF-kappaB activation in BMMs and inhibited RANKL induced formation of TRACP ( + ) multinucleated cells ... The inhibition of NF-kappaB suppresses osteoclastogenesis by downregulation of NFATc1, suggesting that NFATc1 expression is regulated by NF-kappaB in RANKL induced osteoclastogenesis
Zhu et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2005 : The results indicated that deltaN IkappaBalpha may inhibit the activation of NF-kappaB and reduce the release of TNF-alpha and IL-6, suggesting the recombinant deltaN IkappaBalpha adenovirus may be used for anti-inflammatory therapy
Santiago-Lomelí et al., Life Sci 2005 (Chronic Disease...) : IL-10 did not have any effect on the signaling cascade of apoptosis but caused a significant increase in NF-kappaB activation
Tas et al., Eur J Immunol 2005 : NF-kappaB inhibition by the NBD peptide resulted in blockade of IKK mediated IkappaBalpha phosphorylation and subsequent nuclear translocation and DNA binding of NF-kappaB p65 in DC
Ruben et al., Genes Dev 1992 : Transfection experiments demonstrate that I-Rel suppresses NF-kappa B-induced transcription, probably through its association with p50
Hu et al., J Neurochem 2005 (Disease Models, Animal...) : We used a 7-day-old rat model to elucidate the role of nuclear factor-kappaB (NF-kappaB) activation in HI stimulation of IL-1 signaling ... The observed increase in cytoplasmic phosphorylated inhibitor kappaBalpha ( IkappaBalpha ) and nuclear translocation of Bcl-3 24 h after HI was also significantly attenuated by IL-1Ra blockade, suggesting that HI-induced IL-1 activation of NF-kappaB is via both the degradation of IkappaBalpha and the nuclear translocation of Bcl-3
Yang et al., Acta Anaesthesiol Scand 2005 (Drug-Induced Liver Injury) : NF-kappaB inhibitors stabilize CAT-2 and CAT-2B mRNA in LPS stimulated rat liver
Sae-Ung et al., Eur J Haematol 2005 : In contrast, NF-kappaB was undetectable in the nucleus in the presence of IFN-gamma
Chino et al., Int Immunopharmacol 2005 (MAP Kinase Signaling System) : Immunoblotting with phosphorylation-state specific antibodies demonstrated that TJ-48 differentially affected LPS induced phosphorylation of NF-kappa B and MAPKs ... In PEMs treated with TJ-48, LPS induced phosphorylation of p65 NF-kappa B and p38 MAPK was augmented, while that of JNK and ERK was attenuated compared with those in vehicle PEMs
Lentschat et al., J Immunol 2005 : Using the wasp venom derived peptide, mastoparan, to disrupt G protein coupled signaling, we identified a G protein dependent signaling pathway in HMEC stimulated with TLR4 agonists that is necessary for the activation of p38 phosphorylation and kinase activity, NF-kappaB and IL-6 transactivation , and IL-6 secretion
Wright et al., J Cell Biochem 1992 : Furthermore, TNF could still activate NFkB in this variant, suggesting that this pathway is not involved in TNF mediated cytotoxicity
Won et al., Glia 2005 : The inhibition of cPLA2 by methyl arachidonyl fluorophosphates ( MAFP ) or antisense oligomer against cPLA2 and inhibition of iPLA2 by bromoenol lactone reduced the LPS induced iNOS gene expression and NFkappaB activation ... The inhibition of cPLA2 by methyl arachidonyl fluorophosphates ( MAFP ) or antisense oligomer against cPLA2 and inhibition of iPLA2 by bromoenol lactone reduced the LPS induced iNOS gene expression and NFkappaB activation ... Because NDGA could abrogate the LPS induced activation of NFkappaB , while MK886 had no effect on it, LO-mediated inhibition of iNOS gene induction by LPS may involve an NFkappaB dependent or -independent ( by 5-LO ) pathway ... In contrast to LO, however, the cyclooxygenase (COX) may not be involved in the regulation of LPS mediated induction of iNOS gene because COX inhibition by indomethacin ( general COX inhibitor ), SC560 ( COX-1 inhibitor ), and NS398 ( COX-2 inhibitor ) affected neither the LPS induced iNOS expression nor activation of NFkappaB ... In contrast to LO, however, the cyclooxygenase (COX) may not be involved in the regulation of LPS mediated induction of iNOS gene because COX inhibition by indomethacin ( general COX inhibitor ), SC560 ( COX-1 inhibitor ), and NS398 ( COX-2 inhibitor ) affected neither the LPS induced iNOS expression nor activation of NFkappaB
König et al., J Cell Biol 2005 : Overexpression of a constitutively active ALK1 mimicked the effect of TGF-beta1 on NF-kappaB activation and neuroprotection ... Our data suggest that TGF-beta1 simultaneously activates two distinct receptor pathways in neurons and that the ALK1 pathway mediates TGF-beta1 induced NF-kappaB survival signaling
Kim et al., J Pharmacol Exp Ther 2005 : SC-236 suppressed nuclear factor (NF)-kappaB activation induced by PMACI, leading to suppression of IkappaB-alpha phosphorylation and degradation
Peng et al., J Leukoc Biol 2005 : IgE + TNP induced phosphorylation of IKKalpha was accompanied with phosphorylation and degradation of IkappaBalpha , subsequent NF-kappaB activation , and TNF production ... Inhibition of NF-kappaB activity by pyrrolidine dithiocarbomate ( PDTC ) blocked IgE + TNP induced IkappaBalpha synthesis
Costantini et al., J Mol Neurosci 2005 (Neuroblastoma) : Finally, we demonstrated that NF-kappaB activation is dependent on p38 and JNK activation
Wang et al., J Am Soc Nephrol 2005 (Disease Models, Animal...) : Smad7 mediated inhibition of NF-kappaB activation via the induction of IkBalpha may be the central mechanism by which latent TGF-beta prevents renal inflammation
Shi et al., Am J Physiol Gastrointest Liver Physiol 2005 : The overexpression of p65 NF-kappaB enhanced the constitutive expression of ICAM-1 , and TNF-alpha treatment had no further effect
Fahy et al., Cancer Chemother Pharmacol 2005 (Breast Neoplasms...) : The effect of mutation of a NF-kappaB site in the BCL-2 promoter was determined, as was the effect of inhibition of NF-kappaB function using a 26S proteasome inhibitor ( bortezomib ) on both BCL-2 transcription and induction of apoptosis
Cooper et al., Mol Carcinog 2005 : Expression of the leucine zipper domain of the TAM67 protein inhibited UVB induced NF-kappaB transactivation but not AP-1 transactivation
Chen et al., J Huazhong Univ Sci Technolog Med Sci 2004 : To investigate the effect of N-tosyl-L-phenylalanylchloromethyl ketone ( TPCK ) on tumor necrosis factor-alpha induced NF-kappaB activation and apoptosis in U937 cell line, changes and subcellular localization of NF-kappaB/p65 and IkappaB-alpha were observed by fluorescencemicroscopy and expression and degradation of IkappaB-alpha by flow cytometry
Larousserie et al., Am J Pathol 2005 (Epstein-Barr Virus Infections...) : EBI3 is expressed at high levels in EBV transformed B-cell lines and is induced in vitro by the EBV oncogene LMP1 in a nuclear factor (NF)-kappaB dependent manner
Najjar et al., J Virol 2005 (Burkitt Lymphoma...) : Inhibition of NF-kappaB activity by different pharmacological inhibitors ( i.e., parthenolide, MG132 and BAY 11-7082 ) and by overexpressed mutated IkappaBalpha prevented the activation of STAT1
Häussler et al., American journal of physiology. Renal physiology 2005 : We provide evidence for a direct link between EGF, nuclear factor-kappaB (NF-kappaB) , and cell cycle regulation ( cyclin D1 ) ... Moreover, inhibition of NF-kappaB caused a pronounced reduction of EGF induced cyclin D1 promoter activity ... Furthermore, both EGF mediated NF-kappaB activation and cyclin D1 expression were inhibited by coexpression of super IkappaB
Keshamouni et al., Neoplasia (New York, N.Y.) 2005 (Carcinoma, Non-Small-Cell Lung...) : Similarly, an inhibitor of NF-kappa B activation ( PDTC ) also blocked CXCL8 , CXCL5, and CXCL1 production, consistent with their NF-kappa B-dependent regulation ... Similarly, an inhibitor of NF-kappa B activation ( PDTC ) also blocked CXCL8, CXCL5 , and CXCL1 production, consistent with their NF-kappa B-dependent regulation ... Furthermore, PPAR-gamma activation inhibited NF-kappa B transcriptional activity, as assessed by TransAM reporter gene assay ... Furthermore, PPAR-gamma activation inhibited NF-kappa B transcriptional activity, as assessed by TransAM reporter gene assay
Lu et al., J Leukoc Biol 2005 : The role of NF-kappaB in regulation of MMP-9 was demonstrated further by the inhibition of MMP-9 production by proteasome inhibitors, lactacystin and MG-132, which prevented the ubiquitination and dissociation of IkappaB from NF-kappaB
Stalińska et al., J Physiol Pharmacol 2005 : We used human hepatoma ( HepG2 ) and human umbilical vein endothelial cells ( HUVEC ) as a model system : NF-kappaB and AP-1 were activated by the proinflammatory cytokine IL-1 in the absence or presence of 21 selected plant extracts and the effect was evaluated by the electrophoretic mobility shift assay ( EMSA ) ... In both types of cells activation of NF-kappaB by IL-1 was significantly inhibited by extracts from Scandix australis and Artemisia alba, whereas extracts from Amaranthus sp., Eryngium campestre, Thymus pulegioides and Reichardia picroides elicited cell-type dependent response
Linderson et al., Int J Colorectal Dis 2005 (Crohn Disease...) : We sought to determine whether CARD15 mediated NF-kappaB activation can contribute to MDP induced TNFalpha production and, consequently, if polymorphisms in both genes affect the control of such induction ... We sought to determine whether CARD15 mediated NF-kappaB activation can contribute to MDP induced TNFalpha production and, consequently, if polymorphisms in both genes affect the control of such induction ... Transfection and electrophoretic mobility shift assays ( EMSA ) experiments in HEK293 cells demonstrated that MDP exposure stimulates TNFalpha gene transcription, as a result of CARD15 induced NF-kappaB activation and binding to TNFalpha promoter ... Transfection and electrophoretic mobility shift assays ( EMSA ) experiments in HEK293 cells demonstrated that MDP exposure stimulates TNFalpha gene transcription, as a result of CARD15 induced NF-kappaB activation and binding to TNFalpha promoter
Kim et al., Carcinogenesis 2005 (Prostatic Neoplasms) : Proteosome inhibitor-1 (Pro1) reduced NF-kappaB activation, and this inhibition was accompanied by increased levels of cytoplasmic IkappaBalpha and p65/RelA
Zhang et al., Arterioscler Thromb Vasc Biol 2005 (Vasculitis) : We examined the signaling mechanism whereby Ang II activates NF-kappaB in vascular smooth muscle cells ( VSMCs ) ... Downregulation of RSK by small interfering RNA ( SiRNA ) in VSMCs was found to suppress Ang II-induced activation of NF-kappaB and p65 phosphorylation
Sugii et al., Biol Pharm Bull 2005 : Treatments of xanthoangelol D but not xanthoangelol, xanthoangelol E and F markedly suppressed both of basal and tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activation in PAECs
Park et al., Biol Pharm Bull 2005 : Compound K also reduced the expression levels of the inducible NO synthase (iNOS) and COX-2 proteins and inhibited the activation of NF-kB , a nuclear transcription factor ... Compound K also reduced the expression levels of the inducible NO synthase (iNOS) and COX-2 proteins and inhibited the activation of NF-kB , a nuclear transcription factor
Ghosh et al., J Biol Chem 2005 (MAP Kinase Signaling System...) : MBP-1 expression also modulates MEK5 mediated activation of NF-kappaB
Liu et al., Am J Physiol Lung Cell Mol Physiol 2005 : Furthermore, HIV-1 Tat protein enhanced DNA binding activity of NF-kappaB , facilitated nuclear translocation of NF-kappaB subunit p65, and increased production of reactive oxygen species ( ROS ) ... Similarly to VCAM-1 expression, HIV-1 Tat protein induced NF-kappaB activation and ROS generation were abrogated by PDTC and SB-203580
Fickl et al., Clin Exp Immunol 2005 (Hemolysis) : Exposure of neutrophils to pneumolysin was accompanied by influx of Ca ( 2+ ), activation of NFkappaB , and synthesis of IL-8 , all of which were eliminated by inclusion of the Ca ( 2+ ) -chelating agent, EGTA ( 10 m m ), in the cell suspending medium, as well as by pretreatment of the cells with docosahexaenoic acid ( 5 and 10 microg/ml )
Lee et al., Biochem Biophys Res Commun 2005 : SAA stimulated the activation of nuclear factor kappaB (NF-kappaB) , which was required for the MMP-9 upregulation by SAA
Bulut et al., J Biol Chem 2005 (Inflammation) : Mycobacterial heat shock protein 65-induced NF-kappaB activation was MyD88-, TIRAP-, TRIF-, and TRAM dependent and required the presence of MD-2
Blumberg et al., J Interferon Cytokine Res 2005 : Extensions of these findings revealed that the Ctx A subunit induces transcriptional activation of proinflammatory genes in addition to TNF-alpha ( interleukin-8 [ IL- 8 ], intracellular adhesion molecule-1 [ ICAM-1 ], and IL-6 ) and that such transactivation is mediated by the transcriptional regulator NF-kappaB ... Extensions of these findings revealed that the Ctx A subunit induces transcriptional activation of proinflammatory genes in addition to TNF-alpha ( interleukin-8 [ IL- 8 ], intracellular adhesion molecule-1 [ ICAM-1 ], and IL-6 ) and that such transactivation is mediated by the transcriptional regulator NF-kappaB
Lee et al., Clin Cancer Res 2005 (Stomach Neoplasms) : An in vitro study showed that NF-kappaB activity in gastric cancer cells is controlled by and controls Akt
Kaiser et al., J Immunol 2005 : The domain that was required for induction of apoptosis could activate NF-kappa B but not IFN regulatory factor-3, yet the activation of NF-kappa B could be blocked by superrepressor I kappa B alpha without blocking apoptosis
Siebert et al., Arthritis Rheum 2005 : TNFalpha induced activation of NF-kappaB and AP-1 was decreased in the primary dermal fibroblasts with the C43S TNFRSF1A mutation
Pfuhl et al., Hum Genet 2005 (Mammary Neoplasms, Animal) : As the DGCR6 protein ( s ) influence gene expression in trans, we analysed the influence of DGCR6/DGCR6L on the Epstein-Barr virus encoded oncoproteins EBNA2 and EBNA3c in the activation of the viral LMP1 promoter, as well as LMP1 mediated activation of NFkB , but found no effect in either setting
Nam et al., Atherosclerosis 2005 (Arteriosclerosis...) : Upstream of NF-kappaB activation, tilianin inhibited IkappaB kinase activation and the subsequent phosphorylation and degradation of IkappaBalpha protein
Jiang et al., Respir Med 2005 : We therefore evaluated the effects of pyrrolidine dithiocarbamate ( PDTC ; 0.1 mM ) and spermine NONOate ( Sper-NO ; 1 mM ) on adhesion molecule expression and nuclear factor kappa B (NF-kappaB) activation induced by TNF-alpha ( 10 ng/ml ) in cultured human pulmonary microvascular endothelial cells ( PMVEC ) ... The mRNA expression of E-selectin, ICAM-1 and VCAM-1, and activation of NF-kappaB induced by TNF-alpha for 2 h were decreased significantly by the above two pretreatments
Kanazawa et al., J Bone Miner Res 2005 (Bone Resorption) : Only a few TRACP ( + ) multinucleate cells were formed, and TNF-alpha mediated activation of JNK, NF-kappaB , and NFATc1 was defective
Facchini et al., J Cell Physiol 2005 : To search for new approaches to limit cartilage damage, we investigated the requirement of polyamines for NF-kappaB activation by TNFalpha in human C-28/I2 chondrocytes, using alpha-difluoromethylornithine ( DFMO ), a specific polyamine biosynthesis inhibitor
Cario et al., Mol Immunol 2005 : This brief review summarizes the current understanding of Toll-like receptor ( TLRs ) mediated intestinal epithelial mechanisms of commensal tolerance versus intolerance and provides an update on the downstream negative control of signaling responses through decreased surface expression, interregulation with NOD2, overexpression of Tollip , various inhibitors of NF-kappaB as well as soluble tolerizing mediators present in lumen and serum which all may maintain or -- when dysregulated -- impair mucosal homeostasis in health or disease, respectively
Woo et al., Pharmacol Res 2005 : Amentoflavone inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB activation in macrophages ... We isolated amentoflavone from Selaginella tamariscina ( Selaginellaceae ) and studied its effects on nuclear factor-kappaB (NF-kappaB) mediated inducible nitric oxide synthase (iNOS) gene expression in RAW 264.7 cells ... The LPS induced activation of NF-kappaB was also found to be significantly blocked by amentoflavone, but AP-1 activation was unaffected ... NF-kappaB activation is controlled by the phosphorylation and subsequent degradation of I-kappaBalpha , and the cytosolic degradation of I-kappaBalpha was found to be inhibited by amentoflavone
Reich et al., J Am Soc Nephrol 2005 : One pathway involves the activation of NFkappaB by albumin , and it has been demonstrated that the activation of NFkappaB induced by albumin is dependent on mitogen activated protein kinase ERK1/ERK2 ... One pathway involves the activation of NFkappaB by albumin, and it has been demonstrated that the activation of NFkappaB induced by albumin is dependent on mitogen activated protein kinase ERK1/ERK2
Song et al., J Cereb Blood Flow Metab 2005 (Brain Ischemia) : However, SOD 1 prevented NF-kappaB activation, and phosphorylation and degradation of IkappaBalpha after tFCI ... However, SOD 1 prevented NF-kappaB activation, and phosphorylation and degradation of IkappaBalpha after tFCI
Brat et al., Neuro Oncol 2005 (Brain Neoplasms...) : The potential tumor suppressor ING4 was recently shown to be a critical regulator of NF-kappaB mediated IL-8 transcription and subsequent angiogenesis in gliomas
Ohsugi et al., Carcinogenesis 2005 (Disease Models, Animal...) : To examine the effect of a novel NF-kappaB inhibitor, dehydroxymethylepoxyquinomicin ( DHMEQ ), on ATL in vivo, we developed an improved severe combined immunodeficiency (SCID) mouse model for ATL
Esteban et al., Circ Res 2005 : In these cells, Ang IV increased NF-kappaB DNA binding activity, caused nuclear translocation of p50/p65 subunits, cytosolic IkappaB degradation and induced NF-kappaB dependent gene transcription ... Ang II activates NF-kappaB via AT1 and AT2 receptors, but AT1 or AT2 antagonists did not inhibit NF-kappaB activation caused by Ang IV
Li et al., Zhonghua Er Ke Za Zhi 2005 (Incontinentia Pigmenti) : In IP, mutations in NEMO lead to the complete loss of NF-kB activation creating a susceptibility to cellular apoptosis in response to TNF-alpha
Yu et al., Int J Biochem Cell Biol 2005 : NADPH oxidase inhibitor diphenylene iodonium ( DPI ), antioxidant rebamipide, and antisense oligonucleotides ( AS ODNs ) for NADPH oxidase subunits p22phox and p47phox were used to determine the involvement of NADPH oxidase in NF-kappaB activation and IL-6 expression in AR42J cells
Das et al., J Biol Chem 2005 : Activated Tpl2 phosphorylates MKK4/SEK1 upstream of JNK and stimulates NF-kappaB DNA binding and transcriptional activity by mechanisms that are independent of the nuclear translocation of p50 and p65
Komura et al., Cancer Res 2005 (Primary Myelofibrosis) : The regulation of TGF-beta1 synthesis is poorly known but seems regulated by nuclear factor kappaB (NF-kappaB) ... The expression of an NF-kappaB superrepressor in FKBP51 overexpressing cells and in derived megakaryocytes from CD34 ( + ) of IMF patients revealed that NF-kappaB activation was not involved in the resistance to apoptosis after cytokine deprivation of these cells but in TGF-beta1 secretion
Dai et al., Ann Rheum Dis 2005 (Arthritis...) : In chondrocytes responding to IL18, IL18 ( 1-100 ng/ml ) slightly increased the production of MMP-1, MMP-3 , and MMP-13, which was blocked by NF-kappaB inhibitor and p38 mitogen activated protein kinase inhibitor ... In chondrocytes responding to IL18, IL18 ( 1-100 ng/ml ) slightly increased the production of MMP-1 , MMP-3, and MMP-13, which was blocked by NF-kappaB inhibitor and p38 mitogen activated protein kinase inhibitor ... In chondrocytes responding to IL18, IL18 ( 1-100 ng/ml ) slightly increased the production of MMP-1, MMP-3, and MMP-13 , which was blocked by NF-kappaB inhibitor and p38 mitogen activated protein kinase inhibitor ... In chondrocytes responding to IL18, IL18 ( 1-100 ng/ml ) slightly increased the production of MMP-1, MMP-3 , and MMP-13, which was blocked by NF-kappaB inhibitor and p38 mitogen activated protein kinase inhibitor
Lv et al., Acta Anaesthesiol Scand 2005 : Similarly, HES could inhibit hepatic NF-kappaB and AP-1 activations
Onyango et al., Neurobiol Dis 2005 (Alzheimer Disease...) : NF-kappa B activation and cybrid viability are enhanced by NAC
Bernasconi et al., J Biol Chem 2005 : Moreover, NF-kappaB was not essential for virus replication but triggered the expression of proinflammatory cytokines in infected lung cells and was directly responsible for production of high levels of interleukin-8 , a chemokine associated with influenza induced inflammation and airway pathology
Thiefes et al., J Biol Chem 2005 (Inflammation) : Expression of this protein inhibited tumor necrosis factor (TNF) induced activation of NFkappaB , JNK, and p38 MAPK and sensitized the cells to TNF induced apoptosis
Parodi et al., J Vasc Surg 2005 (Aortic Aneurysm, Abdominal...) : MMP-9 is one of the enzymes involved in aneurysm disease, and its production may be induced in part by activation of the transcription factor NF-kappaB
Peng et al., Zhonghua Wai Ke Za Zhi 2005 (Reperfusion Injury) : LPS following IRI could up-regulate CD14 and TLR4 gene and protein expression on Kupffer cells, and subsequently activate NF-kappaB to produce cytokines, but other signal transduction pathways might also participate in the IRI
Ackerman et al., Biol Reprod 2005 : We and others have demonstrated an essential role for nuclear factor kappaB (NFkappaB) in cytokine-driven COX2 expression
Dohrman et al., J Immunol 2005 : Cellular FLIP ( long form ) regulates CD8+ T cell activation through caspase-8 dependent NF-kappa B activation
Guo et al., J Immunol 2005 : To identify a molecular basis for this receptor cross-talk, we examined ERK activation and NF-kappaB induction
Minhajuddin et al., J Immunol 2005 : Inhibition of mTOR by this approach promoted whereas over-expression of mTOR inhibited thrombin induced transcriptional activity of NF-kappaB , an essential regulator of ICAM-1 transcription ... Inhibition of mTOR by this approach promoted whereas over-expression of mTOR inhibited thrombin induced transcriptional activity of NF-kappaB , an essential regulator of ICAM-1 transcription
Yamagishi et al., Cell Tissue Res 2005 : Ang II significantly induced redox-sensitive transcriptional factor NF-kappaB activation and subsequent monocyte chemoattractant protein-1 expression in human umbilical vein ECs ( HUVEC ), both of which were completely inhibited by PEDF or the anti-oxidant N-acetylcysteine
Feng et al., Wei Sheng Wu Xue Bao 2005 : These findings suggest that Pseudomonas pyocyanin can increase IL-8 expression by human airway epithelial cells through MAPKs signaling pathways and the activation of NF-kappaB is also involved in this process
Ko et al., FEBS Lett 2005 : PAF increased mRNA expression, protein synthesis, and MMP-9 activity in ECV304 cells, in a NF-kappaB dependent manner ... PAF increased mRNA expression, protein synthesis, and MMP-9 activity in ECV304 cells, in a NF-kappaB dependent manner ... PAF increased MMP-9 promoter activity in ECV304, which was inhibited by WEB2107, and NF-kappaB inhibitors ... These data indicate that NF-kappaB dependent MMP-9 plays a key role in PAF induced angiogenesis
Doyle et al., J Biol Chem 2005 : Treatment of the murine monocytic cell line Raw264.7 with LFM-A13, a specific Btk inhibitor, blocked LPS induced NFkappaB dependent reporter gene expression but not IkappaB alpha degradation
Dossing et al., J Cell Biochem 2005 (Bone Neoplasms...) : Receptor activator of NF-kappaB ligand protein expression in UMR-106 cells is differentially regulated by parathyroid hormone and calcitriol
Yang et al., Invest Ophthalmol Vis Sci 2005 : TNF-alpha regulates expression of some of these factors in an NF-kappaB dependent manner, whereas others are not influenced by NF-kappaB
Li et al., Zhonghua Yi Xue Za Zhi 2005 (Cardiomegaly) : MyD88 dependent NFkappaB signaling is a novel pathway for inducing the development of cardiac hypertrophy in vivo and blocking MyD88 mediated signaling pathway attenuates the development of cardiac hypertrophy
Lo et al., J Surg Res 2005 (Diabetes Mellitus, Experimental...) : LPS stimulation induced NFkappaB activation that was attenuated by hyperglycemia in RAW cells
Szászi et al., Free Radic Biol Med 2005 : Readdition of glutathione following DEM treatment restored the ability of LPS to induce NF-kappaB translocation and ICAM-1 synthesis
Itoh et al., J Biol Chem 2005 (Inflammation) : Mitochondrial Dok-4 recruits Src kinase and regulates NF-kappaB activation in endothelial cells ... Finally, Dok-4 enhanced TNF-alpha mediated NF-kappaB activation, whereas this was inhibited by transfection with Dok-4 small interfering RNA ... Finally, Dok-4 enhanced TNF-alpha mediated NF-kappaB activation, whereas this was inhibited by transfection with Dok-4 small interfering RNA ... In addition, Dok-4 induced NF-kappaB activation was also inhibited by transfection of a dominant negative form of c-Src ... These data suggest a role for mitochondrial Dok-4 as an anchoring molecule for the tyrosine kinase c-Src, and in turn as a regulator of TNF-alpha mediated ROS production and NF-kappaB activation
Morigi et al., Am J Pathol 2005 (Chronic Disease...) : These data suggest that reorganization of the actin cytoskeletal network in response to protein load is implicated in modulation of the ET-1 gene via Rho kinase dependent FAK activation of NF-kappaB and Ap-1 in differentiated podocytes
Xu et al., Oncogene 2005 (Prostatic Neoplasms) : Analogous to treatments with SFN and PEITC, SR-IkappaBalpha also strongly inhibited NF-kappaB transcriptional activity as well as VEGF, cylcin D1, and Bcl-X ( L ) expression
Heo et al., J Med Food 2005 : The results in this study indicated that SNL glycoprotein induces apoptosis through modulation of PKCalpha and NF-kappaB activity in MCF-7 cells ... In fact, SNL glycoprotein interfered with PKCalpha membrane translocation and inhibited NF-kappaB ( p50 ) protein activity in MCF-7 cells stimulated with TPA ( 61.68 ng/mL, 100 nM ) dose-dependently
Lawrence et al., Nature 2005 (Inflammation...) : The IkappaB kinase (IKK) complex contains two catalytic subunits, IKKalpha and IKKbeta, and controls the activation of NF-kappaB transcription factors, which play a pivotal role in inflammation ... IKKalpha contributes to suppression of NF-kappaB activity by accelerating both the turnover of the NF-kappaB subunits RelA and c-Rel, and their removal from pro-inflammatory gene promoters
Liu et al., Sheng Wu Gong Cheng Xue Bao 2005 (Neoplasms) : The signal transduction pathway leading to NF-kappaB activation via IKK in response to proinflammatory factors like TNF-alpha and IL-1 is addressed in more detail concerning the regulation of IKK activity, mechanism of IkappaB degradation and regulation of transactivation activity of NF-kappaB on different levels
Han et al., Blood 2005 : This RANKL-responsive region contained an NF-kappaB site that, when mutated, completely abolished the induction of NF-kappaB DNA binding activity by RANKL
Nishio et al., Blood 2005 (Leukemia, Lymphocytic, Chronic, B-Cell) : However, BAFF, but not APRIL , also induced CLL-cell activation of NF-kappaB2 ... However, BAFF , but not APRIL, also induced CLL-cell activation of NF-kappaB2
Sahar et al., Circ Res 2005 (Arteriosclerosis...) : Pretreatment of VSMC with Ang II enhanced IL-18 induced NF-kB activation and cytokine gene expression
Morgan et al., Am J Respir Cell Mol Biol 2005 : MBP1 induced activation of neural nuclear factor (NF)-kappaB, from 10 min to 12 h, declining by 24 h, whereas EDN induced a short lived activation of NF-kappaB ... MBP1 induced activation of neural nuclear factor (NF)-kappaB, from 10 min to 12 h, declining by 24 h, whereas EDN induced a short lived activation of NF-kappaB
Wong et al., Am J Respir Cell Mol Biol 2005 : IL-25 could also activate the JNK, p38 MAPK, and NF-kappaB activities of eosinophils, while inhibitor of IkappaB-alpha phosphorylation ( BAY11-7082 ), JNK ( SP600125 ), and p38 MAPK ( SB203580 ) could suppress the release of IL-8, MIP-1alpha, MCP-1, and IL-6
Pinlaor et al., Int J Parasitol 2005 (Inflammation) : These results suggest that O. viverrini induces inflammatory response through TLR2 mediated pathway leading to NF-kappaB mediated expression of iNOS and COX-2 ... These results suggest that O. viverrini induces inflammatory response through TLR2 mediated pathway leading to NF-kappaB mediated expression of iNOS and COX-2
Matsuzawa et al., Nat Immunol 2005 (MAP Kinase Signaling System...) : Here we used ASK1-deficient mice to show that ASK1 was selectively required for lipopolysaccharide induced activation of p38 but not of Jnk or the transcription factor NF-kappaB
Wung et al., J Biomed Sci 2005 : ECs infected with Ad-RacN17 attenuated the TNFalpha induced NFkappaB binding activity
Klingenberg et al., Eur J Immunol 2005 (Acute Disease) : IL-1alpha and TNF-alpha induced ICOSL in an NF-kappaB dependent manner on human umbilical vein endothelial cells ( HUVEC ) ... IL-1alpha and TNF-alpha induced ICOSL in an NF-kappaB dependent manner on human umbilical vein endothelial cells ( HUVEC ) ... IL-1alpha and TNF-alpha induced ICOSL in an NF-kappaB dependent manner on human umbilical vein endothelial cells ( HUVEC )
Horie et al., Fertil Steril 2005 : The present study demonstrates for the first time that P and progestational compounds attenuate the expression of IL-8 by reducing TNFalpha induced NF-kappaB activation in endometriotic stromal cells, suggesting a possible molecular mechanism of hormone therapy for controlling the growth of endometriosis
Cicek et al., Cancer Res 2005 (Breast Neoplasms...) : Further, we showed that suppression of both constitutive and tumor necrosis factor-alpha induced NF-kappaB activation by BRMS1 may be due to inhibition of IkappaBalpha phosphorylation and degradation ... Further, we showed that suppression of both constitutive and tumor necrosis factor-alpha induced NF-kappaB activation by BRMS1 may be due to inhibition of IkappaBalpha phosphorylation and degradation
Chu et al., Acta Anaesthesiol Taiwan 2005 : This study was, thus, conducted to further explore the role of nuclear factor-kappaB (NF-kappaB) in regulating the expression of CAT-2 isozymes in lipopolysaccharide (LPS) treated rat kidney ... In contrast, renal CAT-2A expression was not affected by either LPS or NF-kappaB inhibitors
Gironella et al., Gut 2005 (Colitis...) : The effects of exogenous PAP on nuclear factor kappaB (NFkappaB) activation, proinflammatory cytokine production, and endothelial adhesion molecule expression were also analysed ex vivo ... PAP prevented TNF-alpha induced NFkappaB activation in monocytic, epithelial, and endothelial cells and reduced proinflammatory cytokine mRNA levels and adhesion molecule expression ... PAP inhibits NFkappaB activation and downregulates cytokine production and adhesion molecule expression in inflamed tissue
Feng et al., Mol Cell Biol 2005 : The LIM protein Ajuba influences interleukin-1 induced NF-kappaB activation by affecting the assembly and activity of the protein kinase Czeta/p62/TRAF6 signaling complex ... A prominent function of p62 is the regulation of NF-kappaB activation in response to interleukin-1 (IL-1) and tumor necrosis factor signaling through the formation of an aPKC/p62/TRAF6 multiprotein signaling complex ... A prominent function of p62 is the regulation of NF-kappaB activation in response to interleukin-1 (IL-1) and tumor necrosis factor signaling through the formation of an aPKC/p62/TRAF6 multiprotein signaling complex ... Ajuba null mouse embryonic fibroblasts ( MEFs ) and lungs were defective in NF-kappaB activation following IL-1 stimulation, and in lung IKK activity was inhibited ... Overexpression of Ajuba in primary MEFs enhances NF-kappaB activity following IL-1 stimulation ... We propose that Ajuba is a new cytosolic component of the IL-1 signaling pathway modulating IL-1 induced NF-kappaB activation by influencing the assembly and activity of the aPKC/p62/TRAF6 multiprotein signaling complex
Eom et al., Oncogene 2005 : Furthermore, HD doxorubicin induced apoptosis but not LD doxorubicin induced mitotic catastrophe led to transient activation of NF-kappaB and strong, sustained activations of p38 , c-Jun N-terminal kinase, and caspases
Kang et al., Int J Mol Med 2005 : In addition, GA inhibited TNF-alpha induced phosphorylation of p38 MAPK and extracellular regulated kinases (ERK), I kappa B alpha degradation, and NF-kappa B activation ... These results suggest that GA has the inhibitory effects on TNF-alpha induced IL-8 production in the intestinal epithelial cells through blockade in the phosphorylation of MAPKs, following I kappa B alpha degradation and NF-kappa B activation
De Oliveira et al., J Lab Clin Med 2005 (Diabetes Mellitus, Type 2...) : In the presence of insulin ( 10-100 nmol/L ), the stimulation of NF-kappaB protein expression by AGE-BSA ( 100 microg/mL ) was decreased, whereas the expression of PPAR-gamma, protein was enhanced ... In the presence of insulin ( 10-100 nmol/L ), the stimulation of NF-kappaB protein expression by AGE-BSA ( 100 microg/mL ) was decreased, whereas the expression of PPAR-gamma, protein was enhanced ... 15-Deoxyprostaglandin J2, a direct activator of PPAR-gamma, decreased AGE-BSA stimulated NF-kappaB expression
Higuchi et al., Retrovirology 2005 (Leukemia-Lymphoma, Adult T-Cell) : Exogenous stable expression of CD30 in Rat-1 cells constitutively activated NF-kappaB
Campagnuolo et al., Bioorg Med Chem 2005 : Compound 3 proved active in selectively inhibiting the induction of NF-kappaB by tumor necrosis factor-alpha in T cells
Cho et al., Biochim Biophys Acta 2005 : BIC also induces nitric oxide synthase in macrophages through JunB/AP-1 and NF-kappaB activation
Tian et al., Blood 2005 (Lymphoma, B-Cell) : Induction of Bcl10 activity caused rapid activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK), but not activation of extracellular signal regulated kinase ( ERK ) or p38 mitogen activated protein ( MAP ) kinases ... Induction of Bcl10 activity caused rapid activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK), but not activation of extracellular signal regulated kinase ( ERK ) or p38 mitogen activated protein ( MAP ) kinases ... Induction of Bcl10 activity caused rapid activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK), but not activation of extracellular signal regulated kinase ( ERK ) or p38 mitogen activated protein ( MAP ) kinases
Avellino et al., Blood 2005 (Blast Crisis...) : Using a short interfering (si) RNA approach, we demonstrate that FKBP51 , a large immunophilin inhibited by rapamycin, is essential for drug induced NF-kappaB activation in human leukemia
Tracey et al., J Pathol 2005 (Leukemia, Lymphocytic, Chronic, B-Cell...) : In cell lines representing aggressive lymphomas, NF-kappaB inhibition resulted in a decrease in BIRC5/Survivin expression ... Meanwhile, in chronic lymphocytic leukaemia ( CLL ) -derived lymphocytes, NF-kappaB inhibition resulted in a marked decrease in BCL2 expression
Le Page et al., Prostate 2005 (Prostatic Neoplasms) : EGFR and Her-2 regulate the constitutive activation of NF-kappaB in PC-3 prostate cancer cells ... Using several pharmacological ErbB inhibitors, we demonstrate that EGFR and Her-2 are involved in the constitutive activation of NF-kappaB in PC-3 cells through two different mechanisms ... EGFR activates NF-kappaB through the phosphorylation of IkappaBalpha on serines 32/36 thereby influencing the nuclear translocation of the p65 subunit
Vega-Ostertag et al., Arthritis Rheum 2005 : Our data show that aPL induces significant increases in TF transcription, function, and expression, in IL-6 and IL-8 up-regulation, and in iNOS expression on HUVECs and that these processes involve phosphorylation of p38 MAPK and activation of NF-kappaB ... Our data show that aPL induces significant increases in TF transcription, function, and expression, in IL-6 and IL-8 up-regulation, and in iNOS expression on HUVECs and that these processes involve phosphorylation of p38 MAPK and activation of NF-kappaB ... Our data show that aPL induces significant increases in TF transcription, function, and expression, in IL-6 and IL-8 up-regulation, and in iNOS expression on HUVECs and that these processes involve phosphorylation of p38 MAPK and activation of NF-kappaB ... Our data show that aPL induces significant increases in TF transcription, function, and expression, in IL-6 and IL-8 up-regulation, and in iNOS expression on HUVECs and that these processes involve phosphorylation of p38 MAPK and activation of NF-kappaB
Yang et al., J Biol Chem 2005 (Fibrosarcoma) : In the IFN signaling pathway leading to STAT activation, both JAK1 and TYK2 are essential, whereas NF-kappaB activation requires only TYK2
Yang et al., Inflamm Res 2005 (Disease Progression...) : We demonstrated that intravenous administration of endotoxin could provoke significant lung injury, which was characterized by increase of MPO activity and wet/dry weight ratio, TNF-alpha and IL-6 expression and NF-kappa B activation
Tybulewicz et al., Curr Opin Immunol 2005 : Vav1 is required for TCR induced calcium flux, activation of the ERK MAP kinase pathway, activation of the NF-kappaB transcription factor, inside-out activation of the integrin LFA-1, TCR clustering, and polarisation of the T cell
Pan et al., Thromb Haemost 2005 (Disease Models, Animal...) : Samples from the animal model showed that LPS induced NF-kappaB/DNA binding activity and consequent up-regulation of iNOS expression in tissues were abolished by post-administration of YC-1
Ho et al., Am J Physiol Cell Physiol 2005 : In red gastrocnemius muscles, NF-kappaB activity increased 50 % 1-3 h after 60 min of treadmill exercise, returning to baseline by 5 h. Contraction of isolated extensor digitorum longus muscles in vitro increased IKKalpha/beta phosphorylation sevenfold and this was accompanied by a parallel increase in IkappaBalpha phosphorylation
Iwamoto et al., Tohoku J Exp Med 2005 (Body Weight...) : Supershift assays with specific antibodies revealed that p50, but not p52, p65 , Rel B, or c-Rel, may be involved in the activation of NF-kappaB , though the component primarily responsible for the increase could not be determined ... Supershift assays with specific antibodies revealed that p50, but not p52 , p65, Rel B, or c-Rel, may be involved in the activation of NF-kappaB , though the component primarily responsible for the increase could not be determined ... Supershift assays with specific antibodies revealed that p50, but not p52, p65, Rel B, or c-Rel , may be involved in the activation of NF-kappaB , though the component primarily responsible for the increase could not be determined ... Supershift assays with specific antibodies revealed that p50, but not p52, p65, Rel B , or c-Rel, may be involved in the activation of NF-kappaB , though the component primarily responsible for the increase could not be determined ... Our results indicate that expression of the P-selectin gene is induced through the activation of NF-kappaB and that P-selectin may be involved in the pathogenesis of diabetic nephropathy
Yan et al., Cell Immunol 2004 (Wounds and Injuries) : Inhibiting IkappaBalpha blocked trauma induced NF-kappaB activity, COX-2 expression and PGE2 production
Davis et al., Nitric Oxide 2005 : Effects of mechanistically distinct NF-kappaB inhibitors on glial inducible nitric-oxide synthase expression ... To further understand the role of NF-kappaB in Nos2 expression, we compared three functionally distinct NF-kappaB inhibitors on NF-kappaB transactivation and iNOS induction by rat C6 glial cells
Ho et al., Toxicol Appl Pharmacol 2005 (Lung Neoplasms) : Treatment of the NHBE cells with PD98059, an ERK1/2 ( extracellular signal regulated protein kinase ) -specific inhibitor, specifically suppressed the NNK induced IkappaBalpha phosphorylation at position 32 of the serine residue, suggesting that the ERK1/2 kinase was involved in the IkappaBalpha phosphorylation induced by NFkappaB activation ... To determine whether the NNK induced NFkappaB activation and cyclin D1 induction were also observed in vivo, A/J mice were treated with NNK ( 9.1 mg ) for 20 weeks and the results showed a significant induction of cyclin D1 and NFkappaB translocation determined by immunoblotting analyses
Du et al., Brain Res Mol Brain Res 2005 : NF-(kappa)B mediates amyloid beta peptide stimulated activity of the human apolipoprotein E gene promoter in human astroglial cells ... We examined how A ( beta ) treatment affects APOE expression in cultured cells and tested the role of the transcription factor NF-(kappa)B in APOE gene regulation ... Our data provide evidence that upregulation of APOE by A ( beta ) in astroglial cells is mediated by an NF-(kappa)B-element present in the 5'-flanking region of the APOE gene
Nicolas et al., Dev Biol 2005 (Muscular Atrophy) : We generated transgenic mice carrying a C-terminal deleted form of PW1 ( DeltaPW1 ) which blocks p53 mediated cell death and TNFalpha mediated NFkappaB activation fused to the myogenin promoter
Selvaraj et al., J Leukoc Biol 2005 (MAP Kinase Signaling System...) : Selective inhibitors of the extracellular signal regulated kinase ( ERK ) 1/2 pathway and p38 mitogen activated protein kinase ( MAPK ), but not Jun N-terminal kinase, significantly reduced the activation of NF-kappaB and the production of cytokines and chemokines induced by OmpA- E. coli, indicating a role for these kinases in the NF-kappaB/cytokine pathway
Zou et al., J Cell Physiol 2005 : We conclude that NF-kappaB signal plays a key role in LIGHT mediated upregulation of CD86 expression
Kim et al., Biochem Pharmacol 2005 : Furthermore, the compound inhibited LPS mediated nuclear translocation of NF-kappaB p65 and DNA binding activity of NF-kappaB complex, in parallel, but did not influence LPS mediated IkappaBalpha degradation ... Taken together, QGR compound suppressed LPS mediated NF-kappaB activation, specifically to nuclear localization step of NF-kappaB p65, which was attributable to its down-regulatory action on LPS induced NO production and iNOS expression
Ding et al., J Cardiovasc Pharmacol 2005 : In addition, ESM also significantly inhibited TNFalpha induced translocation of nuclear factor kappaB (NF-kappaB) from cytoplasm to nuclei in endothelial cells
Singh et al., Oncogene 2005 (Breast Neoplasms) : HDAC inhibitors synergized with TRAIL by inducing DRs DR4/TRAIL-R1 and DR5/TRAIL-R2 through NFkappaB activation and some of the proapoptotic members of the Bcl-2 family, and engaging the mitochondrial pathway ... HDAC inhibitors synergized with TRAIL by inducing DRs DR4/TRAIL-R1 and DR5/TRAIL-R2 through NFkappaB activation and some of the proapoptotic members of the Bcl-2 family, and engaging the mitochondrial pathway ... HDAC inhibitors synergized with TRAIL by inducing DRs DR4/TRAIL-R1 and DR5/TRAIL-R2 through NFkappaB activation and some of the proapoptotic members of the Bcl-2 family, and engaging the mitochondrial pathway ... HDAC inhibitors synergized with TRAIL by inducing DRs DR4/TRAIL-R1 and DR5/TRAIL-R2 through NFkappaB activation and some of the proapoptotic members of the Bcl-2 family, and engaging the mitochondrial pathway ... HDAC inhibitors synergized with TRAIL by inducing DRs DR4/TRAIL-R1 and DR5/TRAIL-R2 through NFkappaB activation and some of the proapoptotic members of the Bcl-2 family, and engaging the mitochondrial pathway
Nadjar et al., Biol Psychiatry 2005 (Body Weight) : To assess whether this action is mediated by NFkappaB activation, rats were injected into the lateral ventricle of the brain with a specific inhibitor of NFkappaB activation, the NEMO Binding Domain (NBD) peptide that has been shown previously to abolish completely IL-1beta induced NFkappaB activation and Cox-2 synthesis in the brain microvasculature ... These findings strongly support the hypothesis that IL-1beta induced NFkappaB activation at the blood-brain interface is a crucial step in the transmission of immune signals from the periphery to the brain that underlies further events responsible of sickness behavior
Kim et al., J Pharm Pharmacol 2005 (Disease Models, Animal...) : Furthermore, MEMO inhibited the nuclear factor kappa B (NF-kappaB) activation induced by LPS , and this was associated with the prevention of degradation of the inhibitor kappaB ( IkappaB ), and subsequently with attenuated p65 protein in the nucleus ... Taken together, the data demonstrate that MEMO has anti-inflammatory and antinociceptive activity, inhibiting iNOS, COX-2 and TNF-alpha expression by down regulating NF-kappaB binding activity ... Taken together, the data demonstrate that MEMO has anti-inflammatory and antinociceptive activity, inhibiting iNOS, COX-2 and TNF-alpha expression by down regulating NF-kappaB binding activity ... Taken together, the data demonstrate that MEMO has anti-inflammatory and antinociceptive activity, inhibiting iNOS , COX-2 and TNF-alpha expression by down regulating NF-kappaB binding activity ... Taken together, the data demonstrate that MEMO has anti-inflammatory and antinociceptive activity, inhibiting iNOS, COX-2 and TNF-alpha expression by down regulating NF-kappaB binding activity
Cao et al., Biol Reprod 2005 : Stimulation of macrophage migration inhibitory factor expression in endometrial stromal cells by interleukin 1, beta involving the nuclear transcription factor NFkappaB ... Curcumin ( 10 ( -8 ) M ), which is known for inhibiting NFkappaB activation, inhibited IL1B induced MIF secretion as well as NFkappaB nuclear translocation and DNA binding
Ding et al., Helicobacter 2005 (Helicobacter Infections) : We have previously shown that H. pylori induced NF-(kappa)B activation and interleukin (IL)-8 secretion are mediated by Toll-like receptor (TLR) 2 in epithelial cells
Park et al., J Ethnopharmacol 2005 : Furthermore, MEIO inhibited the LPS induced DNA binding activity of nuclear factor-kappaB (NF-kappaB) , and this was associated with the prevention of inhibitor kappaB degradation and a reduction in nuclear p65 protein levels
Lee et al., Circ Res 2005 (Arteriosclerosis) : Moreover, UCP2 inhibited the increase in ROS production and NF-kappaB activation, and apoptosis of HAECs induced by lysophophatidylcholine ( LPC ) and linoleic acid ... Moreover, UCP2 inhibited the increase in ROS production and NF-kappaB activation, and apoptosis of HAECs induced by lysophophatidylcholine ( LPC ) and linoleic acid
Zhang et al., EMBO Rep 2005 : Using IKK(beta)-deficient bone-marrow derived macrophages and mouse embryo fibroblasts ( MEFs ), we show that IKK(beta) is essential for IKK and NF-(kappa)B activation as well as efficient IL-6 induction ... Using IKK(beta)-deficient bone-marrow derived macrophages and mouse embryo fibroblasts ( MEFs ), we show that IKK(beta) is essential for IKK and NF-(kappa)B activation as well as efficient IL-6 induction
Shimomura-Shimizu et al., Biochem Biophys Res Commun 2005 : LPS induced nuclear translocation of NF-kappaB , which is necessary for the expression of IFN-beta and iNOS, was inhibited by these chemicals : however, the LPS induced degradation of IkappaB-alpha and IkappaB-beta was inhibited only by alachlor ... These results indicate that alachlor and carbaryl differentially impair the LPS induced NF-kappaB activation, leading to the inhibition of NO production
Habens et al., Apoptosis 2005 (Cell Transformation, Viral...) : SFZ, but not 5-ASA or SPY, inhibits activation of NF-kB
Maldonado-Bernal et al., Parasite Immunol 2005 : The interaction of LPPG with TLR2 and TLR4 resulted in activation of NF-kappaB and release of interleukin (IL)-10, IL-12p40, tumour necrosis factor (TNF)-alpha, and IL-8 from human monocytes ... The interaction of LPPG with TLR2 and TLR4 resulted in activation of NF-kappaB and release of interleukin (IL)-10, IL-12p40, tumour necrosis factor (TNF)-alpha, and IL-8 from human monocytes
Rodríguez-Ayala et al., Atherosclerosis 2005 (Inflammation...) : In the patient group with high-grade inflammation, stimulation of mononuclear cells with CML modified human serum albumin caused a rapid dose dependent rise ( p < 0.0001 ) in NFkappaB activity that could be completely blocked by an anti-RAGE antibody
Becker et al., Biochem Biophys Res Commun 2005 (Melanoma) : p16INK4a binding and inhibition of the transcription factor NF-kappaB has been shown and is consistent with the reports of abnormally increased NF-kappaB activity in various cancers including melanoma ... Furthermore, whereas wild type p16INK4a strongly inhibits NF-kappaB transcriptional activity, a subset of melanoma associated p16INK4a mutants show reduced NF-kappaB inhibitory function ... p16INK4a does not affect NF-kappaB nuclear translocation or DNA binding, suggesting a mechanism that reduces NF-kappaB transactivation activity
Baumann et al., J Orthop Res 2005 (Osteolysis) : In comparison, LPS induced a much greater activation of NF-kappaB and TNFalpha promoters, and TNFalpha secretion into the supernatant was strongly induced ... In comparison, LPS induced a much greater activation of NF-kappaB and TNFalpha promoters, and TNFalpha secretion into the supernatant was strongly induced
Yang et al., Naunyn Schmiedebergs Arch Pharmacol 2005 : Incubation of HUVECs with ox-LDL ( 100 microg/ml ) for 24 h markedly elevated ADMA, LDH and TNF-alpha in the conditioned medium and significantly increased the activity of NF-kappaB , concomitantly with a significant decrease in the activity of DDAH and the content of NO
Lubin et al., Neuroscience 2005 : Through inhibition studies we found that extracellular signal regulated protein kinase ( ERK ) and phosphatidylinositol-3 kinase (PI3K) couple to basal and kainate mediated NF-kappaB DNA binding activity in area CA3
Jang et al., Free Radic Biol Med 2005 (MAP Kinase Signaling System) : Pharmacologic inhibition of extracellular signal regulated kinase ( ERK ) and p38 mitogen activated protein kinase ( p38 MAPK ) and dominant negative mutation of both enzymes suppressed not only Abeta induced NF-kappaB transactivation but also COX-2 expression and PGE ( 2 ) production
Lee et al., J Biol Chem 2005 : FBI-1 enhances transcription of the nuclear factor-kappaB (NF-kappaB)-responsive E-selectin gene by nuclear localization of the p65 subunit of NF-kappaB ... FBI-1 enhanced NF-kappaB mediated transcription of E-selectin genes in HeLa cells upon phorbol 12-myristate 13-acetate stimulation and overcame gene repression by IkappaB alpha or IkappaB beta ... Confocal microscopy showed that FBI-1 increased NF-kappaB movement into the nucleus and increased the stability of NF-kappaB in the nucleus, which enhanced NF-kappaB mediated transcription of the E-selectin gene ... Confocal microscopy showed that FBI-1 increased NF-kappaB movement into the nucleus and increased the stability of NF-kappaB in the nucleus, which enhanced NF-kappaB mediated transcription of the E-selectin gene
Koon et al., J Pharmacol Exp Ther 2005 : However, the signal transduction pathways by which SP-NK-1R interaction induces NF-kappaB activation and interleukin-8 (IL-8) production are not clear
Migita et al., FEBS Lett 2005 : Although SIN-1 did not enhance the secretions of MMP-1 and 13, SIN-1 induced the NF-kappaB activation, MT1-MMP expression and the secretion of activated MMP-2 in LI90 cells
Li et al., Cell Immunol 2004 : Together with our previous findings, these results suggest that alpha3beta1 integrin binding results in a suppression of the IL-1 signaling pathway leading to the activation of NF-(kappa)B and ultimately IEC cytokine responses
Zeyda et al., Transplant Proc 2005 : We found that lipopolysaccharide (LPS) induced activation of NF-kappaB , as assessed using electromobility shift assay, is markedly inhibited by FK778 in human monocyte derived DCs
Cacicedo et al., Diabetes 2005 (Diabetic Retinopathy) : Furthermore, inhibition of NF-kappaB activation by various means inhibited caspase-3 activation
Swanson-Mungerson et al., J Virol 2005 : Furthermore, LMP2A induces NF-kappaB nuclear translocation independent of BCR cross linking ... Furthermore, LMP2A induces NF-kappaB nuclear translocation independent of BCR cross linking ... Since NF-kappaB is required to bypass tolerance induction, this LMP2A dependent NF-kappaB activation may complete the tolerogenic signal induced by low levels of soluble HEL
Joo et al., J Virol 2005 : Lipopolysaccharide (LPS) activates IkappaB kinase (IKK) signalsome and NF-kappaB , which leads to the expression of cyclooxygenase-2 (COX-2), which catalyzes production of prostaglandins, potent effectors on inflammation and possibly hepatitis ... NF-kappaB activation by either LPS or overexpression of IKKbeta was sufficient to induce robust expression of COX-2, which was markedly suppressed by ectopic expression of HCV core ... NF-kappaB activation by either LPS or overexpression of IKKbeta was sufficient to induce robust expression of COX-2, which was markedly suppressed by ectopic expression of HCV core ... NF-kappaB activation by either LPS or overexpression of IKKbeta was sufficient to induce robust expression of COX-2 , which was markedly suppressed by ectopic expression of HCV core
Yin et al., Immunol Invest 2005 (Arthritis, Experimental...) : Effects of VIP and [ D-P-Cl-Phe ( 6 ), Leu ( 17 ) ] -VIP on NF-kappaB activity were analyzed using luciferase gene reporter assays ... Effects of VIP and [ D-P-Cl-Phe ( 6 ), Leu ( 17 ) ] -VIP on p65NF-kappaB expression of the synovial cells were examined by Western blot
O'Connor et al., Exp Cell Res 2005 (Lymphoma, B-Cell) : Additionally, we found evidence that PIR IkappaBalpha degradation controls constitutive NF-kappaB activation in certain multiple myeloma cells ... Additionally, we found evidence that PIR IkappaBalpha degradation controls constitutive NF-kappaB activation in certain multiple myeloma cells
Vega et al., Biochem Pharmacol 2005 : We also observed that tropisetron is a potent inhibitor of PMA plus ionomycin induced NF-(kappa)B activation but in contrast TNF(alpha) mediated NF-(kappa)B activation was not affected by this antagonist
Hertlein et al., Mol Cell Biol 2005 : In contrast to the defined regulatory interplay between NF-kappaB and IkappaBalpha, much less is known regarding the regulation of IkappaBbeta by NF-kappaB
Jeong et al., Life Sci 2005 : Our observations show that calcium, MAPK activation, HIF-1alpha, and NF-kappaB are necessary for ethanol induced TNF-alpha and TGF-beta1 expression
Méndez et al., J Neurosci Res 2005 : Glutamate activation of Oct-2 in cultured chick Bergmann glia cells : involvement of NFkappaB
Wu et al., World J Gastroenterol 2005 (Helicobacter Infections...) : These effects appear to be mediated through a NF-kappaB and COX-2 mediated pathway, as COX-2 or NF-kappaB inhibitor significantly attenuate the invasiveness of gastric cancer cells and the expressions of MMP-9 and VEGF protein ... These effects appear to be mediated through a NF-kappaB and COX-2 mediated pathway, as COX-2 or NF-kappaB inhibitor significantly attenuate the invasiveness of gastric cancer cells and the expressions of MMP-9 and VEGF protein
Martínez-Flórez et al., J Nutr 2005 : Although the mode of action remains to be clarified, our findings support the view that the mechanism of action is via inhibition of IL-1beta induced NF-kappaB activation
Takatsuna et al., Biomed Pharmacother 2005 : DHMEQ inhibited lipopolysaccharide (LPS) induced activation of NF-kappaB in an electrophoresis mobility shift assay
Garrido et al., Phytother Res 2005 : An aqueous stem bark extract of Mangifera indica ( Vimang ) inhibits T cell proliferation and TNF induced activation of nuclear transcription factor NF-kappaB ... Moreover, the extract prevented TNFalpha induced IkappaBalpha degradation and the binding of NF-kappaB to the DNA
Wissel et al., Respir Res 2005 : C. pneumoniae induced NF-kappaB activity was blocked by TLR4 blocking antibodies
Park et al., Biochemistry 2005 : Inhibition of NF-kappaB by cobrotoxin resulted in reductions in the LPS induced expressions of COX-2, iNOS, cPLA(2), IL-4, and TNF-alpha in astrocytes and in COX-2 expression induced by SNP, LPS, and TNF-alpha in astrocytes ... Inhibition of NF-kappaB by cobrotoxin resulted in reductions in the LPS induced expressions of COX-2, iNOS, cPLA(2), IL-4 , and TNF-alpha in astrocytes and in COX-2 expression induced by SNP, LPS, and TNF-alpha in astrocytes ... Inhibition of NF-kappaB by cobrotoxin resulted in reductions in the LPS induced expressions of COX-2, iNOS , cPLA(2), IL-4, and TNF-alpha in astrocytes and in COX-2 expression induced by SNP, LPS, and TNF-alpha in astrocytes ... Inhibition of NF-kappaB by cobrotoxin resulted in reductions in the LPS induced expressions of COX-2, iNOS, cPLA(2) , IL-4, and TNF-alpha in astrocytes and in COX-2 expression induced by SNP, LPS, and TNF-alpha in astrocytes ... Inhibition of NF-kappaB by cobrotoxin resulted in reductions in the LPS induced expressions of COX-2 , iNOS, cPLA(2), IL-4, and TNF-alpha in astrocytes and in COX-2 expression induced by SNP, LPS, and TNF-alpha in astrocytes
Xiong et al., Zhonghua Shao Shang Za Zhi 2005 : To investigate the expression of myeloid differentiation protein-2 ( MD-2 ) in the human endothelial cells and its role in lipopolysaccharide (LPS) induced NF-kappaB activation in endothelial cells ... The role of MD-2 in LPS induced NF-kappaB activation and IL-8 production were investigated with gene transfection of mutant MD-2 cDNA ( 0.5, 1.0, 2.0 microg ), pEF-BOS vacant vector ( 2.0 microg ) and MD-2 plasmid ( 2.0 microg ) into HUVEC, respectively ... Transfection of mutant MD-2 cDNA could remarkably inhibit LPS induced NF-kappaB activation and IL-8 production in endothelial cells ... MD-2 might play an important role in the LPS induced NF-kappaB activation in HUVECs
Bu et al., FASEB J 2005 (Hyperplasia...) : Collectively, these findings show that the IKKbeta mediated late-phase NF-kappaB activation contributes to intimal hyperplasia and the accompanied vascular inflammatory responses
Keller et al., Oncogene 2005 (Burkitt Lymphoma) : Interestingly, Emu-Myc B cells express reduced levels of cytoplasmic and nuclear NF-kappaB 1 and have reduced Rel/NF-kappaB DNA binding activity, suggesting that Myc mediated repression of NF-kappaB 1 might mediate its proliferative and apoptotic effects on B cells
Byun et al., J Bone Miner Res 2005 (Bone Resorption) : Our data show that TNF-alpha increases intracellular ROS in hBMSC and that TNF-alpha and H2O2 induce apoptosis in hBMSC through the activation of JNK and NF-kappaB
Park et al., Gastroenterology 2005 (Carcinoma, Hepatocellular...) : Interferon-gamma inhibits hepatitis B virus induced NF-kappaB activation through nuclear localization of NF-kappaB inducing kinase ... However, interferon (IFN)-gamma induced NIK nuclear localization and inhibited NF-kappaB activation in HepG2.2.15 cells and in HepG2 cells transfected with pHBV1.2x ... When NIK nuclear localization was inhibited by deletion of nuclear localization signal on NIK, IFN-gamma did not induce the NIK nuclear localization and did not inhibit NF-kappaB activation ... IFN-gamma selectively inhibits HBV mediated NF-kappaB activation
Aksoy et al., Eur J Immunol 2005 : In the same models of DC activation, PI3K inhibition increased DNA binding activity of NF-kappaB , but not interferon response factor (IRF)-3, the key transcription factors required for TLR mediated IFN-beta synthesis ... In the same models of DC activation, PI3K inhibition increased DNA binding activity of NF-kappaB , but not interferon response factor (IRF)-3, the key transcription factors required for TLR mediated IFN-beta synthesis ... Experiments carried out in HEK 293T cells stably expressing TLR3 or TLR4 confirmed that inhibition of PI3K activity enhances NF-kappaB dependent promoters as well as IFN-beta promoter activities without interfering with transcription at the positive regulatory domain III-I ... Furthermore, wortmannin enhanced NF-kappaB activity induced by TRIF overexpression in HEK 293T cells, while overexpression of catalytically active PI3K selectively attenuated TRIF mediated NF-kappaB transcriptional activity ... We conclude that inhibition of PI3K activity enhances TRIF dependent NF-kappaB activity, and thereby increases IFN-beta synthesis elicited by TLR3 or TLR4 ligands
Lv et al., Ann Clin Lab Sci 2005 (Sepsis) : HES also down-regulated pulmonary proinflammatory cytokines ( TNF-alpha, IL-1beta, and IL-6 ) and mRNA expressions ( CINC and P-selectin ), and inhibited pulmonary activities of NF-kappaB and AP-1
Kim et al., Am J Transplant 2005 (Reperfusion Injury) : TNF mediated activation of NF-kappaB has been proposed to be one pathway leading to transcriptional activation of CMV ie gene expression ... Induction of MIEP-lacZ expression was preceded by TNFR independent formation of reactive oxygen species ( ROS ), weak and transient activation of NF-kappaB and strong and sustained activation of AP-1
Thommesen et al., J Biochem Mol Biol 2005 (Colonic Neoplasms) : Distinct differences between TNF receptor 1- and TNF receptor 2-mediated activation of NFkappaB ... The TNFR1 response was inhibited by D609, bromophenacyl bromide ( BPB ), nordihydroguararetic acid ( NDGA ), and by sodium salicylate, while TNFR2 mediated activation of NFkappaB and CMV promoter-enhancer was resistant to these compounds
Dumitriu et al., J Immunol 2005 : HMGB1/RAGE interaction results in downstream activation of MAPKs and NF-kappaB ... HMGB1/RAGE interaction results in downstream activation of MAPKs and NF-kappaB
Esparza et al., J Immunol 2005 : GITR induced activation of NF-kappaB is blocked by A20, an NF-kappaB-inducible protein that interacts with TRAFs and functions in a negative feedback mechanism downstream of other TNFRs. Interestingly, in contrast with its effects on signaling triggered by other TNFRs, our functional studies revealed that TRAF2 plays a novel inhibitory role in GITR triggered NF-kappaB activation
Hall et al., J Immunol 2005 : Furthermore, IkappaBalpha , the inhibitor of the transcription factor NF-kappaB , is degraded
Hidaka et al., J Exp Clin Cancer Res 2005 (Neoplasms) : To analyze whether p53 status mediates IL-8 expression, the effect of wild-type p53 ( wt-p53 ) gene transfer on activation of NF-kappaB was determined in both cell lines ... In contrast, luciferase reporter studies indicated that transcriptional activity of NF-kappaB is suppressed by transfection of wt-p53 ... These results show that wt-p53 gene transfer inhibits IL-8 production and NF-kappaB transcription activity in cancer cells and suggest that constitutive IL-8 production in cancer cells is associated with mutation of p53
Kao et al., Immunology 2005 : The LTA induced formation of an NF-kappaB-specific DNA-protein complex in the nucleus was inhibited by wortmannin, LY 294002, genistein, tyrphostin AG126, and SB 203580 ... Based on those findings, we suggest that LTA might activate the PI3K/Akt pathway through tyrosine kinase to induce p38 MAPK activation, which in turn initiates NF-kappaB activation, and ultimately induces iNOS expression and NO release in RAW 264.7 macrophages
Kim et al., Immunology 2005 : We report that luteolin significantly blocks lipopolysaccharide (LPS) induced IkappaB phosphorylation/degradation, NF-kappaB transcriptional activity and intercellular adhesion molecule-1 ( ICAM-1 ) gene expression in rat IEC-18 cells ... These results indicate that luteolin blocks LPS induced NF-kappaB signalling and proinflammatory gene expression in intestinal epithelial cells and dendritic cells
Fiebeler et al., Heart Fail Rev 2005 (Cardiovascular Diseases) : This production contributes to nuclear factor kappaB (NF-kappaB) activation and the genes regulated by this transcription factor
Soloviev et al., J Orthop Res 2005 (Osteolysis) : Addition of purified NSmase to ANA-1 cell cultures stimulated NFkappaB binding, increased transcriptional activity in cells transfected with NFkappaB responsive promoters, and induced TNFalpha expression
Fritz et al., J Orthop Res 2005 (MAP Kinase Signaling System) : Chemokine IL-8 induction by particulate wear debris in osteoblasts is mediated by NF-kappaB ... Particulate wear debris induced NF-kappaB activation, the major transcriptional regulator of IL-8 and MCP-1 pro-inflammatory genes and, indeed, both IL-8 and MCP-1 chemokine gene expressions were upregulated in titanium particulate stimulated human osteoblasts ... Particulate wear debris induced NF-kappaB activation, the major transcriptional regulator of IL-8 and MCP-1 pro-inflammatory genes and, indeed, both IL-8 and MCP-1 chemokine gene expressions were upregulated in titanium particulate stimulated human osteoblasts
Martin et al., Osteoarthritis Cartilage 2005 : IL-1beta enhanced both AP-1 and NF-kappaB binding, whereas H ( 2 ) O ( 2 ) only activated AP-1 ... IL-1beta enhanced both AP-1 and NF-kappaB binding, whereas H ( 2 ) O ( 2 ) only activated AP-1 ... H ( 2 ) O ( 2 ) pre-treatment decreased the IL-1beta activation of NF-kappaB ... The different regulation of NF-kappaB and AP-1 by H ( 2 ) O ( 2 ) and IL-1beta underlines the distinct roles played by the two transcription factors in the regulation of gene expression
Place et al., Biochem Pharmacol 2005 : HDAC inhibition prevents NF-kappa B activation by suppressing proteasome activity : down-regulation of proteasome subunit expression stabilizes I kappa B alpha ... The selective impact of HDAC inhibitors on TNF-alpha induced NF-(kappa)B activation appears to relate to the fact that the TNF-alpha induced activation of NF-(kappa)B is mediated by the proteasome, whereas NF-kappaB activation by IL-1beta is largely proteasome independent ... The selective impact of HDAC inhibitors on TNF-alpha induced NF-(kappa)B activation appears to relate to the fact that the TNF-alpha induced activation of NF-(kappa)B is mediated by the proteasome, whereas NF-kappaB activation by IL-1beta is largely proteasome independent
Hu et al., J Biol Chem 2005 : NIBP overexpression potentiates tumor necrosis factor-alpha induced NF-kappaB activation through increased phosphorylation of the IKK complex and its downstream I(kappa)B(alpha) and p65 substrates ... Finally, knockdown of NIBP expression by small interfering RNA reduces tumor necrosis factor-alpha induced NF-kappaB activation , prevents nerve growth factor induced neuronal differentiation, and decreases Bcl-xL gene expression in PC12 cells
Prasad et al., J Neurochem 2005 : It inhibits tumor necrosis factor alpha (TNFalpha) induced PI3-kinase, Akt and NF-kappaB activation in these cells
Wu et al., Kidney Int 2005 : LXA ( 4 ) antagonized the phosphorylation of SHP-2 and activity of NF-kappaB induced by TNF-alpha ... Pretreatment of the cells with NF-kappaB inhibitor pyrrolidine dithio-carbamate ( PDTC ) blocked the productions of IL-1beta, IL-6, and activation of NF-kappaB induced by TNF-alpha
Watanabe et al., Blood 2005 (Leukemia, T-Cell...) : We conclude that NF-kappaB is a potential molecular target for treatment and prevention of ATL
Ho et al., Ann N Y Acad Sci 2005 : NF-kappaB DNA binding activity was elevated in response to relaxin , and supershift assay showed activation of both NF-kappaB subunits p50 and p65 ... In conclusion, these data suggest that relaxin induced MMP-9 expression in THP-1 cells involves NF-kappaB activation
Jiang et al., Di Yi Jun Yi Da Xue Xue Bao 2005 : To investigate the effect of Liangge San, a recipe of traditional Chinese herbal medicine, on lipopolysaccharide (LPS) induced nuclear factor kB (NF-kB) activation in mouse macrophages cultured in vitro and explore the signal transduction mechanism of the detoxifying effect of Liangge San
Mahaseth et al., J Lab Clin Med 2005 (Anemia, Sickle Cell...) : Control albumin had no effect on NF-kappaB , VCAM-1, ICAM-1, rolling, adhesion, or vasoocclusion
Melo et al., Scand J Immunol 2005 : Both EPEC-EAF ( ( + ) ) and EPEC-EAF ( ( - ) ), as well as recombinant BfpA ( data not shown ), activated nuclear factor (NF)-kappaB in a similar manner as analysed by the electrophoretic mobility shift assay ( EMSA )
Wu et al., Transplant Proc 2005 : On posttransplant day 7, the serum albumin level significantly improved, the serum total bile acid and alanine aminotransferase levels were significantly lower, the serum interleukin-2 and interferon-gamma levels were significantly lower, and the activation of transcription factor NF-kappaB in peripheral blood T lymphocytes was significantly suppressed in the combination group in comparison with those in the CsA group ( all P < .05 ) or control group ( all P < .01 ), and a histological examination revealed apparent difference in the severity of rejection between the combination group and CsA group ( P < .05 ) or control group ( P < .01 ) ... On posttransplant day 7, the serum albumin level significantly improved, the serum total bile acid and alanine aminotransferase levels were significantly lower, the serum interleukin-2 and interferon-gamma levels were significantly lower, and the activation of transcription factor NF-kappaB in peripheral blood T lymphocytes was significantly suppressed in the combination group in comparison with those in the CsA group ( all P < .05 ) or control group ( all P < .01 ), and a histological examination revealed apparent difference in the severity of rejection between the combination group and CsA group ( P < .05 ) or control group ( P < .01 )
Shetty et al., Mol Cell Biol 2005 (Breast Neoplasms) : Transcription factor NF-kappaB differentially regulates death receptor 5 expression involving histone deacetylase 1 ... Death receptor 5 ( DR5, TRAIL-R2 ) is a proapoptotic protein considered to be a potential target for cancer therapy, and its expression is mediated by NF-kappaB ... The mechanism of NF-kappaB induced DR5 expression is, however, unknown ... Indeed, HDAC inhibitors activate NF-kappaB and p53 and upregulate DR5 expression ... This provides a mechanism for regulating NF-kappaB mediated DR5 expression and could explain the differential roles NF-kappaB plays in regulating apoptosis
Medjane et al., Am J Physiol Lung Cell Mol Physiol 2005 (Cystic Fibrosis) : Higher activation of NF-kappaB was observed in CF cells compared with control cells and was enhanced by LPS
Huang et al., Ann N Y Acad Sci 2005 : The level of HO-1 expression and its promoter activity mediated by resveratrol was attenuated by nuclear factor-kappa B (NF-kappaB) inhibitors, but not by mitogen activated protein kinase ( MAPK ) inhibitors
Chiu et al., J Biomed Sci 2005 : Pre shearing of ECs at HSS significantly inhibited the TNF-alpha induced p65 and p50 mRNA expressions and nuclear factor-kappaB (NF-kappaB)-DNA binding activity
Ko et al., Planta Med 2005 : Similarly, a time related NF-kappaB/p65 nuclear translocation induced by LPS/IFN-gamma was diminished in the presence of laxifolone A ... Similarly, a time related NF-kappaB/p65 nuclear translocation induced by LPS/IFN-gamma was diminished in the presence of laxifolone A ... In summary, these results suggest that inhibition of NF-kappaB p105 degradation in cytoplasm may participate in the abrogation of LPS/IFN-gamma induced NF-kappaB translocation and subsequent NO synthesis by laxifolone A ... In summary, these results suggest that inhibition of NF-kappaB p105 degradation in cytoplasm may participate in the abrogation of LPS/IFN-gamma induced NF-kappaB translocation and subsequent NO synthesis by laxifolone A
Camacho et al., J Immunol 2005 : These findings demonstrate that AhR regulates FasL and NF-kappaB in stromal cells, which in turn plays a critical role in initiating apoptosis in thymic T cells
Min et al., J Immunol 2005 : TNF related activation induced cytokine enhances leukocyte adhesiveness : induction of ICAM-1 and VCAM-1 via TNF receptor associated factor and protein kinase C-dependent NF-kappaB activation in endothelial cells ... RT-PCR analysis and promoter assays revealed that expression of these cell adhesion molecules was transcriptionally regulated mainly by activation of the inflammatory transcription factor , NF-kappaB ... TRANCE induced IkappaB-alpha phosphorylation and NF-kappaB activation via a cascade of reactions involving the TNFR associated factors, phospholipase C, PI3K, and protein kinase C ( PKC-alpha and PKC-zeta )
Fairchild et al., Am J Physiol Cell Physiol 2005 : While HT is generally thought to have anti-inflammatory and cytoprotective effects, we have previously shown that moderate in vitro HT prolongs TNF-alpha production by LPS stimulated mononuclear phagocytes, in part by prolonging TNF-alpha gene transcription and activation of the pleiotropic transcription factor NF-kappaB
Schnyder et al., Cytokine 2005 : IL-17 also inhibited the TNF mediated expression of adhesion molecule VCAM-1, and the NF-kappaB binding to the VCAM-1 promoter-specific site, along with the inhibitor of NF-kappaB, IkappaB-beta ... IL-17 also inhibited the TNF mediated expression of adhesion molecule VCAM-1, and the NF-kappaB binding to the VCAM-1 promoter-specific site, along with the inhibitor of NF-kappaB, IkappaB-beta
Ross et al., J Am Soc Nephrol 2005 (AIDS-Associated Nephropathy...) : Whether the epithelial apoptosis in HIVAN is mediated by NF-kappaB activated Fas ligand expression was investigated here ... These results suggest that NF-kappaB may regulate Fas mediated apoptosis in HIVAN by controlling the expression of Fas ligand in renal epithelium
Rak Min et al., Life Sci 2005 : As a mechanism of the anti-inflammatory action shown by CP compound, suppression of LPS induced activation of both nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) has been documented ... Finally, CP compound could provide an invaluable tool to investigate LPS induced NF-kappaB and AP-1 activation, in addition to its therapeutic potential in NO- and IL-6 associated inflammatory diseases
Takahashi et al., Leuk Res 2005 (Leukemia, Myeloid, Acute) : In this study, we addressed the role of Flt3 in the activation of nuclear factor-kappa B (NF-kappaB) , which is a target molecule of these kinase pathways
Miho et al., Cardiovasc Res 2005 : Although ICAM-1 expression is regulated by NF-kappaB , recent studies have suggested that additional signaling mechanisms may also be involved
Sakai et al., Biochem Biophys Res Commun 2005 (Colorectal Neoplasms) : NFkappaB inhibitory factor IkappaB decreased the luciferase activity, and forced expression of NFkappaB induced MUC6 transcription ... Our results suggest that MUC6 transcription is regulated by NFkappaB and Sp family members
Rath et al., Biochim Biophys Acta 2005 (Burkitt Lymphoma) : In order to study the relationship between the constitutively active NF-kappaB and IkappaB-alpha, a dominant negative mutant IkappaB-alpha ( IkappaB-alphaDN ), lacking the N-terminal 36 amino acids required for the activation of NF-kappaB by tumor necrosis factor-alpha (TNF-alpha) , was expressed in the Daudi cells ... The expression of IkappaB-alphaDN protein did not inhibit the constitutive NF-kappaB activity, but it inhibited cell proliferation, antiproliferative response to IFN-alpha, and phosphorylated mitogen activated protein kinase ( p-MAPK ) level ... Thus, our results suggest that constitutive NF-kappaB activity in the human Burkitt lymphoma Daudi cells is maintained by a mechanism independent of IkappaB-alpha degradation, and that the IkappaB-alpha is involved in the proliferation of these cells, possibly through the MAP kinase pathway
Chao et al., Am J Physiol Heart Circ Physiol 2005 : Fas associated death-domain protein inhibits TNF-alpha mediated NF-kappaB activation in cardiomyocytes ... Fas associated death-domain protein inhibits TNF-alpha mediated NF-kappaB activation in cardiomyocytes ... Although FADD has previously been reported to play an important role in CM apoptosis, the effect of FADD on CM NF-kappaB signaling, which is a proinflammatory pathway, has not been delineated ... To investigate the role of FADD in CM NF-kappaB activation, we utilized adenoviral gene transfer of wild-type FADD and a truncation mutant that lacks the death-effector domain ( FADD-DED ) in rat CMs in vitro TNF-alpha activated NF-kappaB in CMs as demonstrated by phosphorylation and degradation of inhibitory-kappaB (IkappaB)-alpha enhanced nuclear p65 and NF-kappaB DNA binding activity as well as increased mRNA for the NF-kappaB dependent adhesion molecule VCAM-1 ( 19 +/- 4.1-fold ) as measured by quantitative RT-PCR ... To investigate the role of FADD in CM NF-kappaB activation, we utilized adenoviral gene transfer of wild-type FADD and a truncation mutant that lacks the death-effector domain ( FADD-DED ) in rat CMs in vitro TNF-alpha activated NF-kappaB in CMs as demonstrated by phosphorylation and degradation of inhibitory-kappaB (IkappaB)-alpha enhanced nuclear p65 and NF-kappaB DNA binding activity as well as increased mRNA for the NF-kappaB dependent adhesion molecule VCAM-1 ( 19 +/- 4.1-fold ) as measured by quantitative RT-PCR ... Interestingly, FADD-DED exhibited a similar but weaker inhibitory effect on NF-kappaB activation ... The effects of FADD on NF-kappaB were cell-type specific ... FADD expression also inhibited TNF-alpha mediated NF-kappaB activation in human endothelial cells but not in rat pulmonary artery smooth muscle cells ... FADD expression also inhibited TNF-alpha mediated NF-kappaB activation in human endothelial cells but not in rat pulmonary artery smooth muscle cells ... In contrast, FADD expression actually activated NF-kappaB in human embryonic kidney (HEK)-293 cells ... In CMs, FADD inhibited NF-kappaB activation as well as phosphorylation of IkappaB-alpha and IkappaB kinase (IKK)-beta in response to cytokine stimulation or expression of the upstream kinases NF-kappaB inducing kinase and IKK-beta ... In CMs, FADD inhibited NF-kappaB activation as well as phosphorylation of IkappaB-alpha and IkappaB kinase (IKK)-beta in response to cytokine stimulation or expression of the upstream kinases NF-kappaB inducing kinase and IKK-beta ... These data demonstrate that FADD inhibits NF-kappaB activation in CMs, and this inhibition likely occurs at the level of phosphorylation and activation of IKK-beta
Hall et al., Am J Physiol Heart Circ Physiol 2005 : Taken together, these data provide compelling evidence for the role of NF-kappaB signaling in TNF-alpha gene expression in heart and highlight the importance of this proinflammatory gene-regulatory pathway as a potential therapeutic target in the management of cytokine induced myocardial dysfunction
Lu et al., Invest Ophthalmol Vis Sci 2005 : The activation of NF-kappaB by IL-1beta was markedly inhibited by both triptolide and dexamethasone, whereas the activity of AP-1 was not affected by either agent
Li et al., Diabetes 2005 : In HASMCs, leptin induced PKC, extracellular signal regulated kinase ( ERK ) 1/2, and nuclear factor-kappaB (NF-kappaB) activation and inhibition of these signaling pathways abrogated HASMC proliferation and MMP-2 expression induced by this hormone ... Treatment of HASMCs with metformin decreased leptin induced ROS production and activation of PKC, ERK1/2, and NF-kappaB
Pizzi et al., Acta Neurochir Suppl (Wien) 2005 : Inhibition of IkappaBalpha phosphorylation prevents glutamate induced NF-kappaB activation and neuronal cell death
Zhang et al., J Nutr 2005 : LGG reduced the TNFalpha induced NFkappaB translocation to the nucleus and lessened the decrease in IkappaB in the cytoplasm ( P < 0.05 )
Stark et al., Mol Cell Biol 2005 : Nucleolar sequestration of RelA ( p65 ) regulates NF-kappaB-driven transcription and apoptosis
Zhang et al., Mol Cell Biol 2005 : In the characterization of the IP10 induced apoptotic pathway, we found that overexpression of IP10 upregulated p53 and resulted in altered expression of p53-responsive genes such as the p21Cip1, p27kip1, NF-kappaB , Bax, and PUMA genes and the mitochondrial translocation of Bax
Kaplan et al., Shock 2005 (Lung Injury...) : These inflammatory events were associated with reduced expression of PPARgamma and with activation of nuclear factor-kappaB (NF-kappaB) in the lung
Hsieh et al., J Cell Physiol 2006 : BK-induced cPLA2 expression might be due to the translocation of NF-kappaB into nucleus which was inhibited by Hoe140, helenalin, and PD98059, implying the involvement of NF-kappaB
Hammar et al., J Biol Chem 2005 : Activation of NF-kappaB by extracellular matrix is involved in spreading and glucose stimulated insulin secretion of pancreatic beta cells ... 804G-ECM induced nuclear translocation of NF-kappaB was weak as compared with that induced by interleukin-1beta ... Transient 804G-ECM induced DNA binding activity of NF-kappaB ( peak at 2 h ) and overexpression of NF-kappaB target genes IkappaB alpha and NF-kappaB1 ( p105 ) ( peak at 4 h ) were observed ... When NF-kappaB was inhibited by an inhibitor of IkappaB alpha phosphorylation ( Bay 11-7082 ) or by a recombinant adenovirus expressing the nonphosphorylatable form of IkappaB alpha, 804G-ECM induced cell spreading and actin cytoskeleton organization were reduced ... This study shows for the first time that ECM induced NF-kappaB activity is necessary in maintaining GSIS, although it does not affect survival of pancreatic beta cells ... The effects of ECM induced NF-kappaB activity contrast with the deleterious effects of cytokine induced NF-kappaB activity
Suliman et al., FASEB J 2005 : In Wt mice, mtDNA depletion was avoided by selective iNOS blockade, and residual mtDNA loss was linked to NF-kappaB dependent TNF-alpha expression
Miyazato et al., J Virol 2005 : Evidence for NF-kappaB- and CBP independent repression of p53 's transcriptional activity by human T-cell leukemia virus type 1 Tax in mouse embryo and primary human fibroblasts ... To address this issue, we have investigated Tax 's effect on p53 's transcriptional activation in IkappaB-kinase-deficient mouse embryonic fibroblasts ( MEFs ) ; some of which are entirely silent for Tax induced NF-kappaB activity
Tai et al., Cancer Res 2005 (Multiple Myeloma) : Immunoblotting analysis specifically showed that PI3-K/AKT, nuclear factor-kappaB (NF-kappaB) , and extracellular signal regulated kinase activation induced by CD40L ( 5 mug/mL ) was inhibited by CHIR-12.12 ( 5 mug/mL )
Kim et al., Clin Exp Immunol 2005 (Translocation, Genetic) : Salvia miltiorrhiza water-soluble extract, but not its constituent salvianolic acid B, abrogates LPS induced NF-kappaB signalling in intestinal epithelial cells ... We report that SME significantly abrogated LPS induced IkappaB phosphorylation/degradation, NF-kappaB transcriptional activity and ICAM-1 gene expression in rat IEC-18 cells
Scherübl et al., Journal of inflammation (London, England) 2005 : Surface expressed hicp75TNFR behaves like hp75TNFR demonstrating susceptibility for TACE induced shedding and NFkappaB activation after TNF binding
Li et al., Life Sci 2005 (Colorectal Neoplasms) : These data indicated that PLCgamma1 played a pivotal role in the migration of human colorectal cancer cell and first demonstrated that upregulation of NF-kappaB binding activity and downregulation of Hsp70 expression were PLCgamma1 dependent in LoVo cells
Gomez et al., Br J Pharmacol 2005 : Lipopolysaccharide (LPS) stimulated a significant increase in iNOS induction and NF-kappaB DNA binding ... These parameters were substantially reduced by overexpression of a wild-type Ikappa-Balpha adenoviral construct ( Ad.Ikappa-Balpha ), confirming a role for NF-kappaB in iNOS induction ... These data suggest that IKKbeta plays a predominant, selective role in the regulation of NF-kappaB dependent induction of iNOS in RASMCs
Tsao et al., Biochem Pharmacol 2005 : LPS induced nuclear factor-kappaB (NF-kappaB) DNA binding and NF-kappaB dependent reporter gene activity were both significantly inhibited by HPB
Zhang et al., J Leukoc Biol 2005 : NF-kappaB mediated IL-8 release was independent of Pneumocystis phagocytosis
Alvarez et al., J Neurochem 2005 (Neuroblastoma) : However, transfection of p65/relA NF-kappaB was not enough to induce COX-2 transcription, suggesting that NF-kappaB was necessary but not sufficient to control COX-2 transcription induced by gp120
Kawanami et al., Atherosclerosis 2006 (Atherosclerosis) : C-reactive protein induces VCAM-1 gene expression through NF-kappaB activation in vascular endothelial cells ... C-reactive protein induces VCAM-1 gene expression through NF-kappaB activation in vascular endothelial cells
Reddig et al., J Biol Chem 2005 : Additionally, suppression of endogenous Nischarin protein with small interfering RNA in PC12 cells enhanced Rac1Q61L and Rac1Q61L-40C activation of NF-kappaB
Birrell et al., Am J Respir Crit Care Med 2005 (Asthma...) : In addition, in an in vivo antigen-driven model of airway inflammation, the IKK-2 inhibitor blocked NF-kappaB nuclear translocation, which was associated with a reduction in inflammatory cytokine gene and protein expression, airway eosinophilia, and late asthmatic reaction, similar in magnitude to that obtained with budesonide
Mangashetti et al., J Immunol 2005 (Bone Resorption...) : Interestingly, IL-4 inhibition of bone resorption occurs through prevention of RANKL induced nuclear translocation of p65 NF-kappaB subunit, and intracellular Ca ( 2+ ) changes ... Thus, we reveal that IL-4 acts directly on mature osteoclasts and inhibits bone resorption by inhibiting NF-kappaB and Ca ( 2+ ) signaling
Hagemann et al., J Immunol 2005 (Breast Neoplasms...) : EMMPRIN and MIF were up-regulated in cocultured tumor cells in a JNK- and NF-kappaB dependent manner ... EMMPRIN and MIF were up-regulated in cocultured tumor cells in a JNK- and NF-kappaB dependent manner
Takada et al., Oncogene 2005 (Neoplasm Invasiveness...) : We found that zerumbone suppressed NF-kappaB activation induced by tumor necrosis factor (TNF) , okadaic acid, cigarette smoke condensate, phorbol myristate acetate, and H2O2 and that the suppression was not cell type specific
Kasperczyk et al., Oncogene 2005 (Neoplasms) : BetA induced NF-kappaB activation involved increased IKK activity and phosphorylation of IkappaB-alpha at serine 32/36 followed by degradation of IkappaB-alpha ... BetA induced NF-kappaB activation involved increased IKK activity and phosphorylation of IkappaB-alpha at serine 32/36 followed by degradation of IkappaB-alpha
Jeong et al., Oncogene 2005 (Cell Transformation, Viral) : Activated AKT regulates NF-kappaB activation, p53 inhibition and cell survival in HTLV-1 transformed cells ... Activated AKT regulates NF-kappaB activation, p53 inhibition and cell survival in HTLV-1 transformed cells ... Blocking AKT with the PI3K/AKT inhibitor LY294002 or AKT SiRNA prevented NF-kappaB activation and inhibition of p53 ... We suggest that AKT plays a role in the activation of prosurvival pathways in HTLV-1 transformed cells, possibly through NF-kappaB activation and inhibition of p53 transcription activity
Yan et al., Oncogene 2005 : Since IKKalpha is activated by NF-kappaB inducing kinase (NIK) , we overexpressed native NIK using an adenovirus vector that resulted in enhanced phospho-IkappaBalpha and nuclear p65 in detached cells compared to control detached cells but did not result in a significantly greater number of cells surviving to 24 h
Yip et al., J Bone Miner Res 2005 (Calcium Signaling) : In this study, we showed a biphasic effect of TG on osteoclast formation and apoptosis through the regulation of ROS production, caspase-3 activity, cytosolic Ca2+, and RANKL induced activation of NF-kappaB and AP-1 activities ... In this study, we showed a biphasic effect of TG on osteoclast formation and apoptosis through the regulation of ROS production, caspase-3 activity, cytosolic Ca2+, and RANKL induced activation of NF-kappaB and AP-1 activities ... At these lower concentrations, TG potentiated ROS production and RANKL induced NF-kappaB activity, but suppressed RANKL induced AP-1 activity and had little effect on ERK phosphorylation
Bucki et al., Biochemistry 2005 : Additionally, TLR dependent NF-kappaB translocation in astrocytes appears to be blocked by gelsolin
Zhang et al., Yao Xue Xue Bao 2005 (Brain Ischemia...) : NFATc may be involved in calcineurin mediated ischemic brain damage and transcription factor NF-kappaB may not be involved
Yang et al., Biochem J 2005 : TNFalpha induces NF-kappaB ( nuclear factor kappaB ) and AP-1 ( activator protein 1 ) nuclear binding activities
Lee et al., Immunology 2005 (Intestinal Diseases...) : Furthermore, BAY11-7082, an inhibitor of NF-kappaB activation, significantly reduced the IL-8 production, NF-kappaB binding activity and IkappaB-alpha degradation induced by V. vulnificus infection ... Furthermore, BAY11-7082, an inhibitor of NF-kappaB activation, significantly reduced the IL-8 production, NF-kappaB binding activity and IkappaB-alpha degradation induced by V. vulnificus infection
Das et al., IUBMB Life 2005 (Disease Progression...) : However, the molecular mechanism by which upstream kinases regulate the OPN induced NFkappaB activation and uPA secretion in human breast cancer cells is not well defined ... OPN also induces NFkappaB activation through phosphorylation and degradation of IkappaBalpha by inducing the IKK activity ... OPN also induces NFkappaB activation through phosphorylation and degradation of IkappaBalpha by inducing the IKK activity ... Taken together, OPN induces NFkappaB activity and uPA secretion by activating PI 3'-kinase/Akt/IKK mediated signaling pathways and further demonstrates a functional molecular link between OPN induced PI 3'-kinase dependent Akt phosphorylation and NFkappaB mediated uPA secretion, and all of these ultimately control the motility and invasiveness of breast cancer cells ... Taken together, OPN induces NFkappaB activity and uPA secretion by activating PI 3'-kinase/Akt/IKK mediated signaling pathways and further demonstrates a functional molecular link between OPN induced PI 3'-kinase dependent Akt phosphorylation and NFkappaB mediated uPA secretion, and all of these ultimately control the motility and invasiveness of breast cancer cells
Elmaleh et al., Int J Mol Med 2005 : Furthermore, exogenously added ATIII induced a G-protein coupled signal transduction process in CD4+ T-cells and inhibited TNF-alpha induced NF-kappaB activation ... Furthermore, exogenously added ATIII induced a G-protein coupled signal transduction process in CD4+ T-cells and inhibited TNF-alpha induced NF-kappaB activation
Takase et al., Kidney Int 2005 (Proteinuria) : Inhibition of renal NF-kappaB activation attenuated the increases in intrarenal angiotensinogen protein ( 2.0-fold in rats with protein overloading and saline injection to 1.3-fold in rats with protein overloading and injection of a truncated form of IkappaBalpha ) and angiotensin II ( 1.8-fold to 1.2-fold ), and angiotensinogen mRNA
Li et al., Kidney Int 2005 (Anoxia) : Inhibition of nuclear factor-kappaB (NF-kappaB) caused a moderate decrease in PAI-1 production under hypoxia
Waris et al., J Virol 2005 : Here, we demonstrate that the HCV induced reactive oxygen species and subsequent activation of NF-kappaB mediate the activation of Cox-2
Le Page et al., Prostate 2005 (Prostatic Neoplasms) : Recent research has highlighted the potential role of EGFR and Her-2 in the constitutive activation of NF-kappaB ( NF-kappaB ) in prostate cancer cells, although the mechanism by which these receptors activate NF-kappaB in these cells remains unclear ... Using pharmacological and genetic approaches we show that in PC-3 cells, EGFR and Her-2 are involved in the constitutive activation of NF-kappaB through two different mechanisms ... EGFR activates NF-kappaB through the PI3K/Akt pathway that leads to the phosphorylation of IkappaBalpha on serines 32 and 36, thereby promoting the nuclear translocation of the p65 subunit
Wu et al., Cardiovasc Res 2005 (Acute Disease...) : Moreover, ghrelin inhibited TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) in HUVECs
Zhang et al., Curr Eye Res 2005 : Presence of the NF-kappaB inhibitor kamebakaurin in culture medium blocked P. aeruginosa induced NF-kappaB activation and inhibited IL-6, IL-8, and TNF-alpha expression and secretion ... Presence of the NF-kappaB inhibitor kamebakaurin in culture medium blocked P. aeruginosa induced NF-kappaB activation and inhibited IL-6 , IL-8, and TNF-alpha expression and secretion ... Presence of the NF-kappaB inhibitor kamebakaurin in culture medium blocked P. aeruginosa induced NF-kappaB activation and inhibited IL-6, IL-8 , and TNF-alpha expression and secretion
Qin et al., Blood 2005 : LPS induces CD40 gene expression through the activation of NF-kappaB and STAT-1alpha in macrophages and microglia ... LPS induces CD40 gene expression through the activation of NF-kappaB and STAT-1alpha in macrophages and microglia ... In this study, we describe that LPS is a strong inducer of CD40 expression in macrophages and microglia, which occurs at the transcriptional level and involves the activation of the transcription factors nuclear factor-kappaB (NF-kappaB) and signal transducer and activator of transcription 1alpha ( STAT-1alpha ) ... These results indicate that both LPS induced NF-kappaB activation and endogenous production of IFN-beta that subsequently induces STAT-1alpha activation play critical roles in the transcriptional activation of the CD40 gene by LPS ... These results indicate that both LPS induced NF-kappaB activation and endogenous production of IFN-beta that subsequently induces STAT-1alpha activation play critical roles in the transcriptional activation of the CD40 gene by LPS
Grech et al., J Biol Chem 2005 : Tumor necrosis factor (TNF) superfamily receptors typically induce both NF-kappaB and JNK activation by recruiting the TRAF2 signal transduction protein to their cytoplasmic domain
Shishodia et al., Biochem Pharmacol 2005 (Lymphoma, Mantle-Cell) : On treatment of cells with curcumin, however, downregulated constitutive active NF-kappaB and inhibited the consitutively active IkappaBalpha kinase (IKK) , and phosphorylation of IkappaBalpha and p65
Xue et al., FEBS Lett 2005 : GSTP1 inhibited LPS induced mitogen activated protein kinases MAPKs including ERK, JNK and p38 as well as NF-kappaB activation dose- and time-dependently in transient transfected and stable transfected cells ... GSTP1 inhibited LPS induced mitogen activated protein kinases MAPKs including ERK, JNK and p38 as well as NF-kappaB activation dose- and time-dependently in transient transfected and stable transfected cells
Kim et al., Cancer Res 2005 (Carcinoma, Hepatocellular...) : Specifically, it is not yet understood how TRAIL controls nuclear factor kappaB (NF-kappaB) activation and overcomes its anti-apoptotic effect ... We explored the regulation of NF-kappaB activity by TRAIL and its role in apoptosis ... Although TRAIL alone did not induce NF-kappaB activity, TRAIL combined with z-VAD significantly increased NF-kappaB activation ... Cleavage-resistant p65D97A further increased NF-kappaB activity in TRAIL treated cells, whereas the COOH-terminal p65 fragment acted as a dominant negative inhibitor ... These results suggest that TRAIL induces NF-kappaB activation, but simultaneously abrogates NF-kappaB activation by cleaving p65, and thereby inhibits the induction of anti-apoptotic proteins such as XIAP, which contributes to the strong apoptotic activity of TRAIL compared with other TNF family members
Jin et al., Cancer Res 2005 (Prostatic Neoplasms) : Treatment of DU145 cells with CD437 resulted in a rapid decrease ( > or = 3 hours ) of IkappaBalpha, which was accompanied by increased translocation of the NF-kappaB subunit p65 from the cytoplasm to the nucleus and increased NF-kappaB DNA binding activity ( > or = 4 hours ) ... The NF-kappaB inhibitor, helenalin, inhibited CD437 induced IkappaBalpha reduction and p65 nuclear translocation
Siegmund et al., Mol Cell Biol 2005 : Overexpression of FLIP ( L ) and FLIP ( S ) inhibited Fas- as well as TRAIL mediated NF-kappaB activation and apoptosis induction in IFN-gamma primed cells suggesting that both responses are coregulated at the level of the DISC
Rao et al., Mol Cell Biol 2005 (Inflammation) : Overexpression of IRAK1c suppressed NF-kappaB activation and blocked IL-1beta induced IL-6 as well as lipopolysaccharide- and CpG induced tumor necrosis factor alpha production in multiple cellular systems
Lee et al., Br J Pharmacol 2005 : Oregonin also showed inhibition of LPS mediated NF-kappaB promoter activity and DNA binding ability, as well as p65 nuclear translocation and phosphorylation
Wullaert et al., Hepatology 2005 (Liver Failure, Acute) : This dual function of NF-kappaB emphasizes the need for therapeutics that can inhibit both TNF induced NF-kappaB activation and cell death ... Indeed, ABIN-1 was found to prevent not only NF-kappaB activation, but also apoptosis of cultured hepatocytes in response to TNF , explaining its protective effect against TNF induced liver failure
Virág et al., Curr Vasc Pharmacol 2005 (Necrosis) : Besides serving as a cytotoxic mediator, PARP-1 is also involved in transcriptional regulation, most notably in the NF kappaB and AP-1 driven expression of inflammatory mediators ... Besides serving as a cytotoxic mediator, PARP-1 is also involved in transcriptional regulation, most notably in the NF kappaB and AP-1 driven expression of inflammatory mediators
Zhao et al., Di Yi Jun Yi Da Xue Xue Bao 2005 : A fusion protein with a molecular weight of about 45 kD was purified, which could interact with anti-RAGE antibody and AGE and block AGE induced nuclear factor (NF)-kB activation in the endothelial cells
Tanaka et al., J Pharmacol Exp Ther 2005 : We found that UVB, IL-1, and TNFalpha induced NF-kappaB activation and then produced MMP-1 and bFGF in HaCaT keratinocytes and skin fibroblasts ... We found that UVB, IL-1 , and TNFalpha induced NF-kappaB activation and then produced MMP-1 and bFGF in HaCaT keratinocytes and skin fibroblasts ... We found that parthenolide could effectively inhibit the gene expression mediated by NF-kappaB and the production of bFGF and MMP-1 from cells overexpressing p65, a major subunit of NF-kappaB ... We found that parthenolide could effectively inhibit the gene expression mediated by NF-kappaB and the production of bFGF and MMP-1 from cells overexpressing p65, a major subunit of NF-kappaB
Yamazaki et al., In Vitro Cell Dev Biol Anim 2005 (Sarcoma, Synovial) : Both NF-kappaB and AP-1 deoxyribonucleic acid binding activities were detectable in SW982 cells by EMSA, and they were induced by interleukin-1beta treatment
Lu et al., Mol Biol Cell 2005 (Helicobacter Infections) : Luciferase reporter gene assays and electrophoretic mobility shift assays showed that full IL-6 transcription required binding sites for nuclear factor-kappaB (NF-kappaB) , cAMP response element ( CRE ), CCAAT/enhancer binding protein (C/EBP), and activator protein (AP)-1
Zeng et al., J Neurochem 2005 (Infection) : 3-OMS also inhibited nuclear factor kappaB (NF-kappaB) binding activity induced by TNF-alpha or by the combination of TNF-alpha and Abeta
Laxmanan et al., J Am Soc Nephrol 2005 : By electrophoretic mobility shift assay, it was confirmed that CD40 signaling induced the transcriptional activation of HO-1 through the binding of NF-kappaB to its promoter ... By electrophoretic mobility shift assay, it was confirmed that CD40 signaling induced the transcriptional activation of HO-1 through the binding of NF-kappaB to its promoter
Mehta et al., J Immunol 2005 (MAP Kinase Signaling System) : Neither overexpression or inhibition of MEK, Ras, or Akt affected HB-EGF mediated inhibition of NF-kappaB activation ... Neither overexpression or inhibition of MEK, Ras, or Akt affected HB-EGF mediated inhibition of NF-kappaB activation
Harhaj et al., IUBMB Life 2005 (Leukemia-Lymphoma, Adult T-Cell) : Tax activation of NF-kappaB is critical for the immortalization and survival of HTLV-I infected T cells
Ramage et al., Inhal Toxicol 2004 : It was also found that this expression of CRP with CB and ufCB treatments was dependent on nuclear factor kappa B (NFkappaB)
Conner et al., Ann N Y Acad Sci 2005 : To gain a better understanding of these enzymes we performed a series of investigations including ( 1 ) in silico comparisons of their promoters ; ( 2 ) real-time PCR analysis of their expression in human tissues ; ( 3 ) effects of hyperglycemia, interleukin-1beta (IL-1beta), and nuclear factor kappa-B (NFkappaB) activation on their mRNA levels ; ( 4 ) effects of small interfering RNA ( siRNA ) suppression of FN3K expression ( knockdown ) in cultured cells and ( 5 ) search of FN3K and FN3KRP homologs in available genomic and EST ( expressed sequence tag ) databases
Yu et al., Cell Death Differ 2006 (Inflammation) : Using a HEK293 cell based reconstitution system that stably expresses ASC and procaspase-1 we demonstrated that neither cryopyrin nor pyrin or their corresponding disease associated mutants could significantly activate NF-kappaB in this system
Billich et al., Cell Signal 2005 : Suppression of SPHK1 activation led to reduction of cytokine induced IkappaBalpha phosphorylation and consequently diminished NFkappaB activity due to reduced nuclear translocation of RelA ( p65 ), explaining the dependence of inflammatory mediator production on SPHK1 activation
Murao et al., Virology 2005 : Tumor necrosis factor ( TNF-alpha ) triggers biphasic activation of the NF-kappaB transcriptional regulator ... In contrast, MC159-TRAF2 associations are more relevant for inhibitory function since mutant MC159 proteins unable to bind TRAF2 also can not inhibit TNF mediated NF-kappaB activation ... This ability was attributed to blockage of the persistent phase of TNF-alpha induced NF-kappaB activation for the following reasons : ( 1 ) the initial phase of NF-kappaB transcriptional activation was not affected by the MC159 protein ; ( 2 ) the MC159 protein inhibited TNF-alpha directed degradation of IkappaBbeta, but not IkappaBalpha ; and ( 3 ) expression of the late NF-kappaB regulated cell genes, TNF-alpha and CCL2, was decreased in the presence of the MC159 protein while transcription of the early NF-kappaB regulated cell gene, CXCL1, was not altered
Kuramoto et al., J Gene Med 2006 : Although lipofection induced TNF-alpha can activate nuclear factor kappaB (NF-kappaB) , which, in turn, increases the transgene expression from plasmid DNA in which any NF-kappaB responsive element is incorporated, no attempts have been made to use such biological responses as NF-kappaB activation against a vector to enhance vector mediated gene transfer
Liu et al., Cytokine 2005 : Taken together, these results suggest that the disruption of NF-kappaB mediated transactivation plays a significant role in ER inhibition of IL-6 gene expression
Murali et al., Proc Natl Acad Sci U S A 2005 (Arthritis, Experimental) : TNF-alpha induced NF-kappaB and p38 kinase activities and clinical symptoms of collagen induced arthritis in mice were all diminished
Kawamura et al., Shock 2005 (Reperfusion Injury) : NF-kappaB activation, CD11b/CD18 expression, and the production of TNF-alpha, IL-8, and IL-6 in monocytes and granulocytes were inhibited by NCR in vitro
Cavin et al., Mol Cancer Res 2005 (Carcinoma, Hepatocellular...) : Lastly, we show that NF-kappaB inhibits c-Myc induced activation of caspase-9 and caspase-3 through up-regulation of the antiapoptotic target genes Bcl-X ( L ) and X-linked inhibitor of apoptosis (XIAP)
Ajuwon et al., J Nutr 2005 : Inhibition of fatty acyl Co-A synthase ( FACS ) with triacsin C suppressed palmitate induced NF-kappaB activation ( P < 0.05 ), but caused an additive increase in palmitate induced IL-6 expression ( P < 0.05 ) ... However, the phosphoinositide-3 kinase (PI3K) inhibitor, wortmannin, alone and additively with palmitate, activated the NF-kappaB reporter gene and induced IL-6 expression ( P < 0.05 ). Palmitate also induced the mRNA expression of tumor necrosis factor alpha (TNFalpha) ( P < 0.05 ), but the increase in mRNA abundance was not reflected in a greater protein concentration in the media ( P > 0.05 )
Witthöft et al., Dig Dis Sci 2005 : Enhanced human beta-defensin-2 (hBD-2) expression by corticosteroids is independent of NF-kappaB in colonic epithelial cells ( CaCo2 )
El Eter et al., Can J Physiol Pharmacol 2005 (Reperfusion Injury) : Moreover, PDTC pretreatment abolished p53 expression and inhibited NF-kappaB translocation ... These results clearly demonstrate that NF-kappaB activation and pro-apoptotic protein p53 induction are involved in gastric I/R injury
Choi et al., J Environ Pathol Toxicol Oncol 2005 : NF-kB inducing kinase (NIK) and TGF-k activated kinase 1 (TAK1), the upstream signaling molecules of NF-kB, are involved in the silica mediated COX-2 expression
Correa et al., Curr Biol 2005 : Zebrafish IkappaB kinase 1 negatively regulates NF-kappaB activity ... Hence, using zebrafish ( Danio rerio ) as a model, we examined the evolutionary role of IKK1 in modulating NF-kappaB ... Here, we report that Ikk1 negatively regulates NF-kappaB by sequestering NEMO from active IKK complexes, indicating that IKK1 can function as a repressor of NF-kappaB
Sasaki et al., Am J Physiol Regul Integr Comp Physiol 2005 (Dehydration...) : Surprisingly, LPS markedly increased the hepatic activation of NF-kappaB , an effect that was significantly enhanced ( rather than reduced ) by pretreatment with an ANG-converting-enzyme (ACE) inhibitor or AT1-receptor antagonist ... Furthermore, the same ACE inhibitor and AT1-receptor antagonist each increased the resting NF-kappaB activity in the liver and spleen, although they had no effect on the LPS induced splenic expression of NF-kappaB ... Furthermore, the same ACE inhibitor and AT1-receptor antagonist each increased the resting NF-kappaB activity in the liver and spleen, although they had no effect on the LPS induced splenic expression of NF-kappaB
Sanlioglu et al., Cancer Gene Ther 2006 (Prostatic Neoplasms) : As amplified Ikappa B kinase (IKK) activity is responsible for the constitutive nuclear factor-kappaB (NF-kappaB) activation leading to uncontrolled cell growth and metastasis, a dual vector approach using both an adenovirus vector ( Ad ) expressing the dominant negative mutant of IKKbeta ( AdIKKbetaKA ) and Ad5hTRAIL was employed to determine if prostate cancer cells were sensitized to TRAIL in the setting of IKK inhibition
Tantivejkul et al., J Cell Biochem 2005 (Prostatic Neoplasms) : PAR1 mediated NFkappaB activation promotes survival of prostate cancer cells through a Bcl-xL dependent mechanism ... Inhibition of p38 and ERK1/2 by SB-203589 and PD-098059, respectively, did not abrogate NFkappaB activity, suggesting an independent induction of NFkappaB by PAR1 stimulation
Loeser et al., Arthritis Rheum 2005 (Osteoarthritis) : Stimulation of RAGE signaling can lead to MAP kinase activation and increased NF-kappaB activity
Pereira et al., Biochem Biophys Res Commun 2005 : The nuclear translocation of p50/p50 complex due to M. leprae treatment correlated with repression of NF-kappaB-driven transcription induced by TNF-alpha
Rao et al., Brain Res Mol Brain Res 2005 : Evidence suggests that neuronal COX-2 gene expression is mainly regulated by the transcription factor nuclear factor kappa-B (NF-kappaB)
Chen et al., Nat Cell Biol 2005 : Ubiquitination regulates at least three steps in the NF-kappaB pathway : degradation of IkappaB ( inhibitor of NF-kappaB ), processing of NF-kappaB precursors, and activation of the IkappaB kinase (IKK)
Chen et al., Cardiovasc Res 2006 : Ang II induced activation of NF-kappaB and AP-1 in untransfected VSMCs, however, Ang II induced significantly higher activities of these pro-inflammatory transcription factors in HSF-1 siRNA transfected cells
Mandal et al., J Biol Chem 2005 (Inflammation) : Most importantly, uteroglobin inhibits FP-mediated NF-kappaB activation and cyclooxygenase-2 gene expression by binding and most likely by sequestering PGF2alpha into its central hydrophobic cavity, thereby preventing FP-PGF2alpha interaction and suppressing the production of inflammatory lipid mediators ... Most importantly, uteroglobin inhibits FP-mediated NF-kappaB activation and cyclooxygenase-2 gene expression by binding and most likely by sequestering PGF2alpha into its central hydrophobic cavity, thereby preventing FP-PGF2alpha interaction and suppressing the production of inflammatory lipid mediators
Wang et al., Cancer Res 2005 (MAP Kinase Signaling System) : Because PTEN is a well-known phosphatase involved in the regulation of phosphatidylinositol 3-kinase (PI-3K)/Akt signaling pathway, taken together with the evidence that PI-3K/Akt plays an important role in the activation of AP-1 and NF-kappaB during tumor development, we anticipate that inhibition of AP-1 and NF-kappaB by tumor suppressor p53 seems to be mediated via PTEN, which may be a novel mechanism involved in anticancer activity of p53 protein ... Because PTEN is a well-known phosphatase involved in the regulation of phosphatidylinositol 3-kinase (PI-3K)/Akt signaling pathway, taken together with the evidence that PI-3K/Akt plays an important role in the activation of AP-1 and NF-kappaB during tumor development, we anticipate that inhibition of AP-1 and NF-kappaB by tumor suppressor p53 seems to be mediated via PTEN, which may be a novel mechanism involved in anticancer activity of p53 protein
Zhang et al., Chin Med Sci J 2005 : Effect of oxidized-LDL on NF-kappaB nuclear translocation in aortic smooth muscle cells originated from rats of different ages
Arima et al., J Immunol 1992 : Using electrophoretic mobility shift assays and DNA-protein cross linking techniques, we demonstrate that IL-2 binding to functional forms of the human IL-2R activates nuclear expression of the eukaryotic transcription factor NF-kappa B ... IL-2 activation of nuclear NF-kappa B expression is regulated in part at a post-translational level, involving the rapid translocation of both the 50- and 65-kDa subunits of NF-kappa B from the cytoplasm to the nucleus ... IL-2 induction of NF-kappa B through the IL-2R beta subunit was both correlated with activation of the endogenous IL-2R alpha gene and critically dependent upon the presence of a serine-rich cytoplasmic domain within IL-2R beta ( amino acid residues 267-312 )
Hung et al., J Toxicol Environ Health 2005 (Adenocarcinoma...) : Moreover, the suppression of BAY, a nuclear factor (NF)-kappaB binding inhibitor, or the COF induced IAP2 protein levels indicated that NF-kappaB activation by COF may partly be involved in IAP2 induction
Lee et al., J Toxicol Environ Health 2005 : Motorcycle exhaust particles induce IL-8 production through NF-kappaB activation in human airway epithelial cells ... Through further, direct detection of nuclear factor (NF)-kappaB activation in epithelial cells using immunoblotting of nuclear p65 and NF-kappaB reporter assay, data showed that MEP induced nuclear translocation of p65 and enhancement of NF-kappaB luciferase gene expression
Samuelsson et al., Int J Mol Med 2005 : beta-Amyloid and interleukin-1beta induce persistent NF-kappaB activation in rat primary glial cells ... We observed that the induction of NF-kappaB binding activity induced by either IL-1beta or Abeta ( 25-35 ) showed a peak at 30 min, and significantly declined after 2 h ... The potentiating effect of Abeta ( 25-35 ) on IL-1beta induced NF-kappaB binding activity was observed after 30 min, 2 h and 24 h, and did not significantly differ over time
Zheng et al., Oncol Rep 2005 : To gain insight into the role of NF-kappaB in the regulation of the vimentin gene during 12-O-tetradecanoylphorbol-13-acetate ( TPA ) -dependent differentiation of HL-60 cells, the effect of pyrrolidine dithiocarbamete ( PDTC ) has been investigated using Northern blot hybridization and DNA mobility shift assay
Li et al., J Biomed Sci 2005 (Inflammation) : In conclusion, TVN efferent stimulation increases substance P release to trigger NF-kappaB mediated ICAM-1 expression and O ( 2 ) ( - ) and H ( 2 ) O ( 2 ) ROS production in the respiratory tract
Wang et al., Sichuan Da Xue Xue Bao Yi Xue Ban 2005 (Colitis, Ulcerative...) : The results indicated that thalidomide treatment significantly reduced colonic inflammation, suppressed NF-kappaB activation, enhanced TNF-alpha mRNA degradation, inhibited the synthesis of the TNF-alpha , IEN-gamma and increased the production of IL-4
Wooten et al., J Biol Chem 2005 : The p62 scaffold regulates nerve growth factor induced NF-kappaB activation by influencing TRAF6 polyubiquitination
Levrand et al., J Biol Chem 2005 (Inflammation) : NF-kappaB activation ( phosphorylation and degradation of its inhibitor IkappaBalpha , nuclear translocation of NF-kappaB p65, and NF-kappaB DNA binding ) triggered by lipopolysaccharide or tumor necrosis factor-alpha was abrogated by peroxynitrite ... At the same time, peroxynitrite activated phosphorylation of NF-kappaB inducing kinase and IKKalpha , considered as part of an alternative, noncanonical NF-kappaB activation pathway ... Suppression of IKKbeta dependent NF-kappaB activation translated into a marked inhibition of the transcription of NF-kappaB dependent genes by peroxynitrite ... Thus, peroxynitrite has a dual effect on NF-kappaB, inhibiting canonical IKKbeta dependent NF-kappaB activation while activating NF-kappaB inducing kinase and IKKalpha phosphorylation, which suggests its involvement in an alternative pathway of NF-kappaB activation ... Thus, peroxynitrite has a dual effect on NF-kappaB, inhibiting canonical IKKbeta dependent NF-kappaB activation while activating NF-kappaB inducing kinase and IKKalpha phosphorylation, which suggests its involvement in an alternative pathway of NF-kappaB activation
Tezel et al., Exp Eye Res 2005 : Comparative gene array analysis of TNF-alpha induced MAPK and NF-kappaB signaling pathways between retinal ganglion cells and glial cells
Chapman et al., Endocrinology 2005 : TNFalpha is a potent activator of NF-kappaB , and levels of this cytokine are increased within the myometrium at term
Thomas et al., J Immunol 2005 : Activation of APCs via TLRs leads to activation of NF-kappaB , a key transcription factor in cells of the immune system most often associated with induction of Th1-type and proinflammatory responses ... This observation led us to ask whether LNFPIII activated APCs were differentially activating NF-kappaB , and if so, could this partly account for how DCs mature in response to these two different pathogen associated molecular patterns ( PAMPs ) ... In this study, we show that LNFPIII-Dex stimulation of APCs induces rapid, but transient NF-kappaB translocation and activity in the nucleus, in comparison with the persistent activation induced by LPS ... We then demonstrate that transient vs persistent NF-kappaB activation has important implications in the development of the APC phenotype, showing that the second wave of NF-kappaB translocation in response to LPS is required for production of the proinflammatory mediator NO
Tang et al., Cancer Biol Ther 2005 : In the March 2005 issue of Science ( 2005 ; 307 : 1465-8 ), Su et al. provide compelling evidence supporting a role for caspase-8 in antigen receptor induced NF-kappaB activation
de Léséleuc et al., Cell Death Differ 2006 : Nur77 overexpression led to enhanced NF-kappaB activity, and DNA binding inhibitors confirmed the contribution of NF-kappaB to Nur77 antiapoptotic activity ... Nur77 overexpression leads to NF-kappaB dependent induction of the antiapoptotic gene cIAP1
Pfeffer et al., Mutat Res 2005 : The interference with the TNFalpha pathway is reflected by reduced NFkappaB activation in anti-oxidants treated cells but the compounds are not able to contrast TNFalpha mediated activation of NFkappaB
Koh et al., Toxicology 2005 (Brain Ischemia...) : We investigated the effect of poly ( ADP-ribose ) polymerase ( PARP ) inhibitor on the levels of plasma and brain matrix metalloproteinase-9 (MMP-9) and the expression of nuclear factor kappa B (NF-kappaB) during experimental focal cerebral ischemia ... Plasma MMP-9 increased maximally at 4h and then decreased rapidly, brain MMP-9 increased maximally at 24 h and persisted until 72 h, and NF-kappaB increased maximally at 24h and then decreased slowly in both groups ... Therefore, the PARP inhibitor reduces the expression of MMP-9 and NF-kappaB and the infiltration of neutrophils in ischemic stroke
Rannou et al., Joint Bone Spine 2006 (Arthritis, Rheumatoid) : When these cytokines bind to their membrane receptor, they set off signaling cascades, with activation of TGFbeta activating kinase ( TAK-1 ), of NF-kappaB by Ikappa-B kinase, of mitogen activated protein kinases ( MAP kinases ), and finally of activator protein-1 (AP-1) ... When these cytokines bind to their membrane receptor, they set off signaling cascades, with activation of TGFbeta activating kinase ( TAK-1 ), of NF-kappaB by Ikappa-B kinase , of mitogen activated protein kinases ( MAP kinases ), and finally of activator protein-1 (AP-1)
Glass et al., Int J Biochem Cell Biol 2005 (Hypertrophy...) : Further, it has been demonstrated recently that activation of the NF-kappaB transcription pathway, activated by cachectic factors such as TNFalpha, is sufficient to induce skeletal muscle atrophy, and this atrophy occurs in part via NF-kappaB mediated upregulation of MuRF1
Yue et al., Proc Natl Acad Sci U S A 2005 : Selective NF-kappaB inhibition blocked this CD1d stimulated IL-12 production
Yamada et al., J Urol 2005 (Urinary Bladder Neoplasms) : Although LPS and IFN-gamma alone marginally activated nuclear factor (NF)-kappaB but not AP-1, LPS plus IFN-gamma augmented NF-kappaB and AP-1 ... Although LPS and IFN-gamma alone marginally activated nuclear factor (NF)-kappaB but not AP-1, LPS plus IFN-gamma augmented NF-kappaB and AP-1 ... TNF-alpha also activated NF-kappaB , AP-1 and ERK1/2
Wu et al., World J Gastroenterol 2005 : On the 7th post-transplant day the serum albumin level significantly improved in the RES C group, the serum total bile acid and alanine aminotransferase ( ALT ) levels were significantly lower in the RES C group, the serum IL-2 and INF-gamma levels were significantly lower in the RES C group, and the activation of transcription factor NF-kappaB in peripheral blood T lymphocytes was significantly suppressed in the RES A, B, and C groups in comparison to those in the control group ... On the 7th post-transplant day the serum albumin level significantly improved in the RES C group, the serum total bile acid and alanine aminotransferase ( ALT ) levels were significantly lower in the RES C group, the serum IL-2 and INF-gamma levels were significantly lower in the RES C group, and the activation of transcription factor NF-kappaB in peripheral blood T lymphocytes was significantly suppressed in the RES A, B, and C groups in comparison to those in the control group
Fyfe et al., Cytokine 2005 (Inflammation...) : Available data suggest that NF-kappaB activation is required for IL-8 gene expression ... Unlike IL-1beta, PAR-2 stimulation did not activate NF-kappaB , which may explain the lack of IL-8 expression
Takahashi et al., Genes Cells 2005 (Pancreatic Neoplasms) : We found that both NF-kappaB activation and Bcl-2 family proteins could prevent the 53BP2S mediated depression of mitochondrial transmembrane potential, activation of caspase-9, cleavage of poly ADP ribose polymerase ( PARP ) , and cell death ... We found that both NF-kappaB activation and Bcl-2 family proteins could prevent the 53BP2S mediated depression of mitochondrial transmembrane potential, activation of caspase-9 , cleavage of poly ADP ribose polymerase ( PARP ), and cell death
Alvarez-Castelao et al., FEBS Lett 2005 : IkappaBalpha regulates activation of the transcription factor NF-kappaB
Chen et al., Int J Biochem Cell Biol 2005 : This study explored the effects of inhibition of endoplasmic reticulum ( ER ) Ca ( 2+ ) -ATPase on lipopolysaccharide (LPS) induced protein kinase C ( PKC ) activation, nuclear factor-kappaB (NF-kappaB) translocation, inducible nitric oxide synthase (iNOS) expression and nitric oxide ( NO ) production in RAW 264.7 macrophages ... Furthermore, NF-kappaB-specific DNA-protein binding activity in the nuclear extracts was enhanced by treatment with LPS, and TG pretreatment attenuated LPS stimulated NF-kappaB activation ... None of DBHQ, A23187 and ionomycin pretreatment reduced LPS stimulated NF-kappaB activation ... These data suggest that persistent inhibition of ER Ca ( 2+ ) -ATPase by TG would influence calcium release from ER Ca2+ pools that was stimulated by the LPS activated signal processes, and might be the main mechanism for attenuating PKC and NF-kappaB activation that induces iNOS expression and NO production
Grumbach et al., J Mol Cell Cardiol 2005 : We hypothesized that this inhibitory action of NO on NFkappaB activation serves as a negative feedback to inhibit NFkappaB activity and eNOS transcription
O'Donnell et al., J Clin Invest 2005 (Myocarditis...) : Treatment of p50-null mice with IFN-beta substantially diminished reovirus replication and apoptosis, which suggests that IFN-beta induction by NF-kappaB protects against reovirus induced myocarditis
Vega et al., Oncogene 2005 (Burkitt Lymphoma...) : The involvement of NF-kappaB and YY1 in the regulation of Fas expression was corroborated by the use of Ramos cells with a dominant-active inhibitor of NF-kappaB ( Ramos IkappaB-estrogen receptor ( ER ) mutant ) and by silencing YY1 with YY1 siRNA, respectively
Lv et al., J Int Med Res 2005 (Endotoxemia...) : HES also decreased the number of MPO positive cells induced by LPS and inhibited activation of NF-kappaB and AP-1
Curran et al., J Biol Chem 2005 (Astrocytoma...) : Using this assay, R ( + ) WIN 55,212-2 strongly inhibited IL-1 activation of NFkappaB ... In addition electrophoretic mobility shift and chromatin immunoprecipitation assays showed that R ( + ) WIN 55,212-2 does not regulate the IL-1 induced nuclear translocation of NFkappaB or the ability of the latter to bind to promoters regulating expression of ICAM-1 and IL-8
Kwon et al., J Biol Chem 2005 (Inflammation...) : These findings suggest that Tax mediated activation of NF-kappaB plays a key role in the development of this aggressive skin disease that shares several features in common with the skin disease occurring during the preleukemic stage in HTLV-I infected patients
Urbinati et al., J Cell Sci 2005 : alpha(v)beta3-integrin dependent activation of focal adhesion kinase mediates NF-kappaB activation and motogenic activity by HIV-1 Tat in endothelial cells ... alpha ( v ) beta3/Tat interaction triggers the activation of NF-kappaB in endothelial cells in a focal adhesion kinase-, RhoA- and pp60src dependent manner, as shown in dominant negative focal adhesion kinase transfectants or using specific pharmacological inhibitors ... Finally, the activation of focal adhesion kinase, RhoA, NF-kappaB and pp60src are required to mediate the motogenic activity of Tat in endothelial cells
Kerbauy et al., Blood 2005 (Myelodysplastic Syndromes) : Exogenous TNF-alpha enhanced NF-kappaB nuclear translocation in MDS BMMCs above baseline levels ... Treatment with arsenic trioxide ( ATO ; 2-200 microM ) inhibited NF-kappaB activity in normal marrow, primary MDS, and ML1 cells, even in the presence of exogenous TNF-alpha ( 20 ng/mL ), and down-regulated NF-kappaB dependent antiapoptotic proteins, B-cell leukemia XL ( Bcl-XL ), Bcl-2, X-linked inhibitor of apoptosis (XIAP), and Fas associated death domain (FADD)-like interleukin-1beta converting enzyme ( FLICE ) inhibitory protein ( FLIP ), leading to apoptosis
Tomita et al., Int J Cancer 2006 (Leukemia, T-Cell...) : Curcumin also inhibited Tax induced NF-kappaB transcriptional activity
Shah et al., Int J Cancer 2006 (Colonic Neoplasms) : Ursodeoxycholic acid inhibits interleukin 1 beta [ corrected ] and deoxycholic acid induced activation of NF-kappaB and AP-1 in human colon cancer cells ... UDCA did not increase DNA binding of NF-kappaB and AP-1 and UDCA pretreatment inhibited DCA induced NF-kappaB and AP-1 DNA binding
Son et al., FEBS Lett 2005 : Histidine also abolished the NF-kappaB dependent activation of the IL-8 promoter induced by TNF-alpha
Yang et al., Hepatology research : the official journal of the Japan Society of Hepatology 2005 : The herb treatment suppressed LPS stimulated TNFalpha release in vivo and by cultured KC. Direct addition of the aqueous herb extract suppressed NF-kappaB activation by KC and TNFalpha promoter activity in RAW cells under LPS stimulation
An et al., Mol Cell Biol 2005 (Kidney Neoplasms) : Recently, VHL loss was shown to activate nuclear factor kappa B (NF-kappaB) , a family of transcription factors that promotes tumor growth
Iwamoto et al., Scand J Gastroenterol 2005 (Helicobacter Infections...) : To evaluate the role of transcription factor NF-kappaB in uPA and uPAR gene transcription with H. pylori stimulation, the effect of NF-kappaB inhibitor MG132 on H. pylori induced uPA and uPAR mRNA expression was examined ... To evaluate the role of transcription factor NF-kappaB in uPA and uPAR gene transcription with H. pylori stimulation, the effect of NF-kappaB inhibitor MG132 on H. pylori induced uPA and uPAR mRNA expression was examined
Chen et al., Proc Natl Acad Sci U S A 2005 (Adenocarcinoma...) : Phosphorylated FADD induces NF-kappaB , perturbs cell cycle, and is associated with poor outcome in lung adenocarcinomas
Brummer et al., Med Mycol 2005 (Inflammation) : We postulated that DEX inhibition of NF-kappaB was opposed by induction of IkappaB kinases (IKK) by GM-CSF + conidia stimulation, degradation of IkappaB, and release of nuclear factor kappa B (NF-kappaB)
Bai et al., Dig Dis Sci 2005 (Colitis, Ulcerative) : DATS could downregulate TNF-alpha production and inhibit NF-kappaB activation in lamina propria mononuclear cells of inflammed mucosa, without any effect on the viability of colonic tissue cells
Corsini et al., Toxicol Appl Pharmacol 2006 : It is known that nuclear factor-kappaB (NF-kappaB) tightly regulates TNF transcription
McCord et al., Infect Immun 2005 : However, MCP-1 production was dependent on NF-kappaB activity ... However, MCP-1 production was dependent on NF-kappaB activity
Ikeda et al., Immunopharmacol Immunotoxicol 2005 : NF-kappaB activation via TLR2 , TLR1/ TLR2, TLR2/TLR6, and TLR4/MD-2/CD14 also was enhanced by stimulation with ZWS and ZWIS ... NF-kappaB activation via TLR2, TLR1/ TLR2, TLR2/TLR6, and TLR4/MD-2/CD14 also was enhanced by stimulation with ZWS and ZWIS ... NF-kappaB activation via TLR2, TLR1/ TLR2, TLR2/TLR6, and TLR4/MD-2/CD14 also was enhanced by stimulation with ZWS and ZWIS ... NF-kappaB activation via TLR2, TLR1/ TLR2, TLR2/TLR6 , and TLR4/MD-2/CD14 also was enhanced by stimulation with ZWS and ZWIS ... NF-kappaB activation via TLR2, TLR1/ TLR2, TLR2/TLR6, and TLR4/MD-2/CD14 also was enhanced by stimulation with ZWS and ZWIS ... NF-kappaB activation via TLR2, TLR1/ TLR2, TLR2/TLR6 , and TLR4/MD-2/CD14 also was enhanced by stimulation with ZWS and ZWIS
Tanifuji et al., Antioxid Redox Signal 2005 : AngII induced MCP-1 protein expression in mProx at 6 h was largely blocked by ROS ( N-acetylcysteine ; 82 +/- 14 % ), Ras ( N-acetyl-S-trans, trans-farnesyl-L-cysteine ; 82 +/- 13 % ), and nuclear factor-kappaB (NF-kappaB) ( parthenolide ; 89 +/- 7.9 % ) inhibitors ... In MC, protein kinase ( calphostin C ; 84 +/- 2.8 % ) and NF-kappaB ( 89 +/- 1.4 % ) inhibitors attenuated acute AngII induced MCP-1 expression stronger than ROS/Ras inhibitors ( 1.0 +/- 0.9/29 +/- 9.5 % )
Tsatsanis et al., Biochem Biophys Res Commun 2005 (Inflammation) : GAd induced NF-kappaB activation and tolerance to further gAd or LPS exposure
Cusson-Hermance et al., J Biol Chem 2005 : Rip1 mediates the Trif dependent toll-like receptor 3- and 4-induced NF-{kappa}B activation but does not contribute to interferon regulatory factor 3 activation ... However, we find that Rip1 expression stimulates NF-kappaB but not interferon regulatory factor 3 activity
Lin et al., Clin Cancer Res 2005 (Adenocarcinoma...) : Function neutralizing antibody to alphavbeta3 but not alphavbeta5 effectively suppressed Cyr61 mediated NF-kappaB activation, COX-2 gene expression, and cell invasiveness
Li et al., Proc Natl Acad Sci U S A 2005 : Increased NFkappaB activity in IKK1 mutant ELDM was the result of prolonged degradation of IkappaBalpha in response to infectious pathogens
Cooper et al., J Immunol 2005 (MAP Kinase Signaling System) : Finally, NF-kappaB activation by the capsid in HEK 293 cells specifically required expression of TLR2 and was compromised by soluble heparin
Uddin et al., J Immunol 2005 (Neurocysticercosis) : Such chemokine secretion is NF-kappaB dependent, likely to involve AP-1, and is regulated in a paracrine loop by monocyte derived TNF-alpha
Youn et al., J Immunol 2005 : Further, resveratrol inhibited NF-kappaB activation induced by TRIF, but not by MyD88 ... The suppressive effect of resveratrol on LPS induced NF-kappaB activation was abolished in TRIF-deficient mouse embryonic fibroblasts, whereas LPS induced degradation of IkappaBalpha and expression of cyclooxygenase-2 and inducible NO synthase were still inhibited in MyD88-deficient macrophages ... Furthermore, resveratrol inhibited the kinase activity of TANK binding kinase 1 and the NF-kappaB activation induced by RIP1 in RAW264.7 cells ... Furthermore, resveratrol inhibited the kinase activity of TANK binding kinase 1 and the NF-kappaB activation induced by RIP1 in RAW264.7 cells
Voleti et al., J Immunol 2005 : In this study we show that NF-kappaB requires and acts synergistically with C/EBPbeta on the CRP-proximal promoter to regulate CRP expression
Jancso et al., J Cardiovasc Pharmacol 2005 (Arrhythmias, Cardiac...) : This study investigated the effect of ASA on the late phase of ischemic preconditioning ( PC ) against myocardial infarction and on the activation of NF-kappaB in the preconditioned myocardium
Wang et al., J Huazhong Univ Sci Technolog Med Sci 2005 (Shock, Septic) : 1 h, 2 h, 4 h and 6 h after LPS injection, the activation of NF-kappaB in blood mononuclear cells and the content of TNF-alpha and IL-6 in plasma was detected by enzyme linked immunoadsordent assay ( ELISA ) ... 1 h, 2 h, 4 h and 6 h after LPS injection, the activation of NF-kappaB in blood mononuclear cells and the content of TNF-alpha and IL-6 in plasma was detected by enzyme linked immunoadsordent assay ( ELISA )
Lim et al., J Med Food 2005 : Taken together, the results of this study showed that the activation of PKCalpha, NF-kappaB , and Bax expression by SNL glycoprotein is possibly involved in the apoptotic process
Völp et al., Gut 2006 (Colonic Neoplasms) : HMGB1 increased NFkappaB activity and led to co-overexpression of the antiapoptotic NFkappaB target gene product c-IAP2 in vitro
Newcomb et al., J Biol Chem 2005 : Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression ... Inhibition of PI 3-kinase and Akt attenuated RV39 induced NF-kappaB transactivation and IL-8 expression
Lora et al., J Biol Chem 2005 (Cystic Fibrosis) : TNF-alpha, but not transforming growth factor-alpha, induced mucus production dependent on IKKbeta mediated NF-kappaB activation ... In vivo, TNF-alpha induced NF-kappaB as determined by whole mouse body bioluminescence
Gookin et al., Am J Physiol Gastrointest Liver Physiol 2006 (Body Weight...) : NF-kappaB mediated expression of iNOS promotes epithelial defense against infection by Cryptosporidium parvum in neonatal piglets ... In acute C. parvum infection, iNOS expression predominated in the villous epithelium, was NF-kappaB dependent , and was not restricted to infected enterocytes
Nakakuki et al., Arterioscler Thromb Vasc Biol 2005 (Atherosclerosis...) : NF-kappaB activity was markedly enhanced by CRP and pretreatment with Y-27632 inhibited its activation
Fukuda et al., Invest Ophthalmol Vis Sci 2005 : Combined stimulation with LPS and either sCD14 or LBP also induced the phosphorylation and degradation of IkappaB-alpha and the translocation of NF-kappaB from the cytoplasm to the nucleus of corneal fibroblasts ... Combined stimulation with LPS and either sCD14 or LBP also induced the phosphorylation and degradation of IkappaB-alpha and the translocation of NF-kappaB from the cytoplasm to the nucleus of corneal fibroblasts
Graham et al., Cell cycle (Georgetown, Tex.) 2005 : NFkappaB activation induces death receptor 5 (DR5) expression following DNA damaging agent etoposide treatment but not following growth factor EGF treatment ... In addition, HDAC inhibitors induce NFkappaB binding to the DR5 gene and DR5 expression, contributing to HDAC inhibitor induced apoptosis
Miyamoto et al., J Endod 2005 : The result of EMSA showed that capsaicin inhibited tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor-kappa B (NF-kappaB) activation in PF-10 cell cultures
Farid et al., Nutrition & metabolism 2005 : Both curcumin, a component of the spice turmeric, and N-acetylcysteine (NAC) , an antioxidant, inhibit activation of NF-kappaB by inflammatory stimuli, albeit by different mechanisms ... In contrast, NAC prevented the increase in NF-kappaB activity induced by unloading but did not prevent losses of muscle mass or function ... In conclusion, neither dietary curcumin nor dietary NAC prevents unloading induced skeletal muscle dysfunction and atrophy, although dietary NAC does prevent unloading induced NF-kappaB activation
Tolosa et al., Cell Signal 2005 : This cytokine induced down-regulation of the subtype 2 of TNF-alpha receptors ( TNF-R2 ), enhanced TNF-alpha induced NF-kappaB translocation to the nucleus and binding to DNA and increased the immunoreactivity of the protein c-IAP1, a member of the inhibitor of apoptosis (IAP) gene family whose synthesis is stimulated by NF-kappaB at the transcriptional level
Higuchi et al., J Control Release 2005 : Intracellular distribution of NFkappaB decoy and its inhibitory effect on TNFalpha production by LPS stimulated RAW 264.7 cells
Seth et al., Cell 2005 (Respirovirus Infections) : Conversely, overexpression of MAVS induces the expression of IFN-beta through activation of NF-kappaB and IRF 3, thus boosting antiviral immunity ... Conversely, overexpression of MAVS induces the expression of IFN-beta through activation of NF-kappaB and IRF 3, thus boosting antiviral immunity
Suzuki et al., Exp Eye Res 2006 (Uveitis) : Immunohistochemical staining with a monoclonal antibody against activated NF-kappaB was performed in order to evaluate the effects of AST on NF-kappaB activation
Nakamura et al., Pathobiology 2005 : The dysregulated overexpression of BCL10 that results from a specific chromosomal translocation t ( 1 ; 14 ) ( p22 ; q32 ) in mucosa associated lymphoid tissue lymphoma has been shown to activate nuclear factor (NF)-kappaB , which may promote growth and survival in tumor cells ... The aim of this study was to determine the molecular mechanisms underlying NF-kappaB activation by BCL10 overexpression ... Furthermore, the BCL10 mediated NF-kappaB activation was efficiently inhibited by a Ca2+ chelating agent or a Ca2+ channel blocker ... These findings suggest that the filamentous pattern of overexpressed BCL10 at the perinuclear region adjacent to the endoplasmic reticulum is important for the BCL10 mediated NF-kappaB activation
Hasel et al., Lab Invest 2005 (Fibrosis...) : TRAIL-R4 signaling in T cells induces NFkappaB , which activates antiapoptotic programs
Clifton et al., Int J Med Microbiol 2005 : Thus, while chemokine response early during the infection likely depends on the direct activation of NF-kappaB, subtle autocrine effects of newly synthesized IL-1alpha may contribute , in part, to the control of NF-kappaB activation and chemokine production at later times
Lee et al., Virology 2005 : Degradation of IkappaB protein was detected late in PRRSV infection, and overexpression of the dominant negative form of IkappaBalpha ( IkappaBalphaDN ) significantly suppressed NF-kappaB activation induced by PRRSV ... Moreover, NF-kappaB dependent expression of matrix metalloproteinase (MMP)-2 and MMP-9 was observed in PRRSV infected cells, an observation which implies that NF-kappaB activation is a biologically significant aspect of PRRSV pathogenesis ... Moreover, NF-kappaB dependent expression of matrix metalloproteinase (MMP)-2 and MMP-9 was observed in PRRSV infected cells, an observation which implies that NF-kappaB activation is a biologically significant aspect of PRRSV pathogenesis
Chen et al., Mol Immunol 2006 : Triptolide inhibits B7-H1 expression on proinflammatory factor activated renal tubular epithelial cells by decreasing NF-kappaB transcription
Xing et al., J Exp Med 2005 : G5pr(-/-) B cells did not show abnormalities in the BCR mediated activation of Erks and NF-kappaB , cyclin D2 induction, or Akt activation
Andersen et al., J Cell Biol 2005 : Functionally, we describe that Ubc13-Mms2 is required for DNA damage repair but not nuclear factor kappaB (NF-kappaB) activation, whereas Ubc13-Uev1A is involved in NF-kappaB activation but not DNA repair ... Functionally, we describe that Ubc13-Mms2 is required for DNA damage repair but not nuclear factor kappaB (NF-kappaB) activation, whereas Ubc13-Uev1A is involved in NF-kappaB activation but not DNA repair
Eto et al., Circulation 2005 : The TNF-alpha induced increase in nuclear factor (NF)-kappaB DNA binding activity was significantly suppressed by TDZD-8 ... GSK-3 regulates TNF-alpha induced IkappaB-alpha degradation and NF-kappaB activation independent of IkappaB-kinase-beta and subsequent induction of TF and VCAM-1 expression in human endothelial cells ... GSK-3 regulates TNF-alpha induced IkappaB-alpha degradation and NF-kappaB activation independent of IkappaB-kinase-beta and subsequent induction of TF and VCAM-1 expression in human endothelial cells
Lai et al., Nephron. Experimental nephrology 2005 (Albuminuria...) : Interleukin-11 reduces renal injury and glomerular NF-kappa B activity in murine experimental glomerulonephritis
Chang et al., Nephron. Experimental nephrology 2005 (Inflammation) : C-reactive protein induces NF-kappaB activation through intracellular calcium and ROS in human mesangial cells ... We investigated the effects of CRP on the nuclear factor-kappaB (NF-kappaB) activation and monocyte chemoattractant protein-1 ( MCP-1 ) gene expression in human mesangial cells and also examined whether intracellular calcium and reactive oxygen species ( ROS ) were involved in the CRP- induced NF-kappaB activation ... CRP increased NF-kappaB binding activity in a dose dependent manner ( 12.5-100 microg/ml ), which was induced within 1 h after incubation and peaked around 3 h. CRP also increased the MCP-1 mRNA expression via activation of NF-kappaB ... Calcium chelator, BAPTA-AM and anti-oxidants such as N-acetylcysteine and tiron suppressed CRP induced NF-kappaB activation
Kuphal et al., Oncogene 2006 (Melanoma...) : Downregulation of NFkappaB activity, either directly or by re-expression of E-cadherin, led to a suppression of N-cadherin promoter activity and N-cadherin expression ... Loss of E-cadherin induces NFkappaB activity and N-cadherin expression in tumorigenic EMT
Kim et al., Pharm Res 2005 (Inflammatory Bowel Diseases) : In human colon epithelial cells, the effect of quercetin on tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor kappa B (NFkappaB) activation was examined ... Furthermore, quercetin dose-dependently inhibited an inflammatory signal TNF-alpha dependent NFkappaB activation ... Our data suggest that rutin acted as a quercetin deliverer to the large intestine and its anti-inflammatory action in TNBS induced colitis rats may be through quercetin mediated inhibition of TNF-alpha induced NFkappaB activation
Bhattacharya et al., Apoptosis 2005 : DN-Akt , as well as the PI3-kinase inhibitors, prevented Akt activation and subsequent translocation of NF-kappaB to the nucleus ... DN-Akt, as well as the PI3-kinase inhibitors, prevented Akt activation and subsequent translocation of NF-kappaB to the nucleus
Fernández et al., J Endocrinol 2005 : Recently, we demonstrated that 3,3',5-triiodothyronine ( T3 ) induces oxidative stress in rat liver, with enhancement in the DNA binding of nuclear factor-kappaB (NF-kappaB) and the NF-kappaB dependent expression of tumor necrosis factor-alpha (TNF-alpha)
Chen et al., Mol Cell Biol 2005 : Reconstitution of RelA-deficient murine embryonic fibroblasts with RelA S276A or RelA S536A decreased TNF-alpha induced acetylation of lysine 310 and expression of the endogenous NF-kappaB-responsive E-selectin gene
Kanno et al., Life Sci 2006 (Disease Models, Animal...) : NG was found to have blocked the LPS induced transcriptional activity of NF-kappaB in electrophoretic mobility shift assay and reporter assay
Wen et al., Life Sci 2006 : From the in vitro experiments, bromelain inhibits lipopolysaccharide (LPS) induced nitrite overproduction in macrophage cell lines and LPS induced NF-kappaB luciferase reporter gene expression in RAW264.7 macrophages transfected with NF-kappaB luciferase reporter gene
Cominacini et al., J Am Coll Cardiol 2005 (Angina, Unstable) : In in vitro studies, the effect of ox-LDL and of the sera derived from a subgroup of UA patients and control subjects on monocytic NF-kB activation was also evaluated ... In the in vitro studies, ox-LDL dose-dependently increased the activation of NF-kB in monocytes, but not in lymphocytes derived from healthy volunteers
Schell et al., J Surg Res 2005 (Inflammation) : Mild heat shock had no effect on LPS induced NF-kappaB activation or IkappaB degradation
Planavila et al., Biochim Biophys Acta 2005 : Electrophoretic mobility shift assay revealed that both parthenolide and atorvastatin significantly decreased LPS stimulated NF-kappaB binding activity in H9c2 cardiac cells ... These results suggest that LPS mediated NF-kappaB activation inhibits the expression of genes involved in fatty acid metabolism by a mechanism involving reduced expression of PGC-1, which in turn affects the PPARbeta/delta transactivation of target genes involved in cardiac fatty acid oxidation
Lee et al., Biochem Biophys Res Commun 2005 (Fibrosarcoma) : We also found that SM-7368 strongly inhibits TNF-alpha induced NF-kappaB activity but not AP-1 activity
Ahmed et al., J Nutr 2005 : PFE also inhibited the IL-1beta induced phosphorylation of IkappaBalpha and the DNA binding activity of the transcription factor NF-kappaB in OA chondrocytes
Lee et al., Cancer Res 2005 (MAP Kinase Signaling System) : Our studies showed that 16-kDa PRL specifically blocked IRF-1 but not NF-kappaB signaling to the iNOS promoter
Lin et al., Biol Pharm Bull 2005 : Therefore, we studied the effects of CRP and lovastatin on NF-kappaB activation in human umbilical vein endothelial cells ( HUVECs ) ... By using an electrophoretic mobility shift assays ( EMSA ), we found that CRP ( 50 microg/ml ) increased activation of NF-kappaB and degradation of inhibitory kappa B ( IkappaB ) in HUVECs, reaching a maximal effect after the incubation with CRP for 1 h. Lovastatin ( 10 ( -5 ) mol/l ) diminished NF-kappaB activation induced by CRP ... Our results suggest that CRP increases activation of NF-kappaB and induces CD40 expression in HUVECs partly via activation of NF-kappaB
Lin et al., Planta Med 2005 : Our results indicate that its ROS scavenger and IKK inhibitory activities also contribute to the suppression of ROS mediated NF-kappaB activity
Yousaf et al., Arthritis Rheum 2005 (Familial Mediterranean Fever) : Tumor necrosis factor receptor I from patients with tumor necrosis factor receptor associated periodic syndrome interacts with wild-type tumor necrosis factor receptor I and induces ligand independent NF-kappaB activation
Hirayama et al., Arthritis Rheum 2005 (Arthritis, Experimental...) : We have previously shown that IL-4 inhibits osteoclastogenesis in vitro through inhibition of NF-kappaB and JNK activation in a STAT-6 dependent manner
Andoh et al., Gastroenterology 2005 (Inflammatory Bowel Diseases) : IL-22 induced an activation of nuclear factor (NF)-kappaB and activating protein (AP)-1 within 1 hour, and a blockade of NF-kappaB and AP-1 activation markedly reduced IL-22 induction of IL-6, IL-8, IL-11, and LIF mRNA
Lee et al., Biochem Pharmacol 2005 (Colonic Neoplasms) : Parallel with inhibitory effect on cell growth, HCA dose dependency inhibited TNF-alpha induced activation of NF-kappaB accompanied with inhibition of the translocation of p50
Purkerson et al., Eur J Immunol 2005 : The TRAF6, but not the TRAF2/3, binding domain was found to regulate IL-8 and IL-6 production, and induce activation of NF-kappaB and Jun kinase in lung fibroblasts, demonstrating for the first time that CD40ct domains can function independently to regulate pro-inflammatory responses of primary human fibroblasts ... The TRAF6, but not the TRAF2/3, binding domain was found to regulate IL-8 and IL-6 production, and induce activation of NF-kappaB and Jun kinase in lung fibroblasts, demonstrating for the first time that CD40ct domains can function independently to regulate pro-inflammatory responses of primary human fibroblasts
Yin et al., Yao Xue Xue Bao 2005 : To investigate the regulatory effects of various anti-inflammatory drugs on both endogenous and TNFalpha induced NF-kappaB activation as well as the relative biological activity ... Dexamethasone ( 1 x 10 ( -8 ) mol.L ( -1 ) ) and meloxicam ( 1 x 10 ( -7 ) - 1 x 10 ( -6 ) mol.L ( -1 ) ) can decrease both endogenous and TNFalpha induced NF-kappaB activation ... However, its influence on TNFalpha induced NF-kappaB activation is needed for further study
Thomas et al., Journal of neuroinflammation 2005 : Lastly, our data show that NGF/TNFalpha promoted iNOS induction requires activation of the transcription factor nuclear factor kappa B (NF-kappaB)
Tian et al., Acta Anaesthesiol Scand 2005 : 3.75 and 7.5 ml/kg HES suppressed LPS induced NF-kappaB activation in the four tissues and decreased plasma TNF-alpha elevation ... 3.75 and 7.5 ml/kg HES suppressed LPS induced NF-kappaB activation in the four tissues and decreased plasma TNF-alpha elevation
Tokuda et al., Connect Tissue Res 2005 : As shown by electrophoretic mobility shift assay p38 MAPK was not involved in SP-induced activation of nuclear factor-kappa B (NF-kappaB)
Yamamoto et al., J Hypertens 2005 : Interleukin-1beta induced NF-kappaB activation in vascular smooth muscle cells, and the addition of GGA further inhibited this NF-kappaB activation
Uchihara et al., Int J Cancer 2006 (Hodgkin Disease) : Thus, in H/RS cells, ligand independent activation of CD30 signalling activates NF-kappaB and this leads to constitutive ICAM-1 expression, suggesting a link between 2 well known phenotypic characteristics of HL, CD30 and ICAM-1 overexpression
Suliburk et al., Surgery 2005 (Endotoxemia...) : LPS increased serum aspartate aminotransferase and alanine aminotransferase levels, hepatic iNOS and COX-2 protein, and nuclear factor NF-kappaB
Kumari et al., Biochem Biophys Res Commun 2005 (Neoplasms, Experimental) : The enhanced NFkappaB activity resulted in the elevation of TRAIL and its receptor DR4 , both known to induce apoptosis
Xu et al., Mol Cell 2005 : Depletion of VISA inhibits virus triggered and RIG-I mediated activation of IRF-3, NF-kappaB , and the IFN-beta promoter, suggesting that VISA plays a central role in virus triggered TLR3 independent IFN-beta signaling
Peng et al., J Surg Res 2006 (Acute Disease...) : Promoter-reporter assay demonstrated that FasL gene promoter was regulated by NF-kappaB
Kim et al., Biochem Biophys Res Commun 2005 (Insulinoma...) : IFN-gamma sensitizes MIN6N8 insulinoma cells to TNF-alpha induced apoptosis by inhibiting NF-kappaB mediated XIAP upregulation
Muceniece et al., J Neuroimmunol 2005 (Disease Models, Animal...) : We found that beta-MSH suppresses LPS induced nuclear translocation of the transcription factor NF-kappaB , and inhibits the expression of inducible nitric oxide synthase , and the following nitric oxide overproduction in the brain, in vivo ... We found that beta-MSH suppresses LPS induced nuclear translocation of the transcription factor NF-kappaB , and inhibits the expression of inducible nitric oxide synthase, and the following nitric oxide overproduction in the brain, in vivo
Chung et al., J Biol Chem 2005 (Insulin Resistance) : Collectively, these data demonstrate for the first time that trans-10, cis-12 CLA promotes NFkappaB activation and subsequent induction of IL-6 , which are at least in part responsible for trans-10, cis-12 CLA mediated suppression of peroxisome proliferator activated receptor gamma target gene expression and insulin sensitivity in mature human adipocytes
Sun et al., Oncogene 2005 (Leukemia-Lymphoma, Adult T-Cell) : Whereas Tax plays a primary role in HTLV-I mediated NF-kappaB activation, recent studies reveal that the IKK/NF-kappaB signaling pathway is also activated in freshly isolated adult T-cell leukemia ( ATL ) cells that often lack detectable Tax expression
Kaur et al., J Biol Chem 2005 (Carcinoma, Hepatocellular) : In addition, induction of NF-kappaB by TGF-beta1 inhibits JNK signaling, thereby attenuating TGF-beta1 induced cell death of normal hepatocytes ... We show that NF-kappaB plays a role in the up-regulation of XIAP gene expression in response to TGF-beta1 treatment and forms a TGF-beta1-inducible complex with TAK1
Rui et al., FASEB J 2005 (Inflammation) : NFkappaB and AP-1 differentially contribute to the induction of Mn-SOD and eNOS during the development of oxidant tolerance ... NFkappaB and AP-1 differentially contribute to the induction of Mn-SOD and eNOS during the development of oxidant tolerance ... In this model, nuclear translocation of both NF-kappaB and AP-1 is required for induction of Mn-SOD , while nuclear translocation of AP-1, but not NF-kappaB, is a prerequisite for induction of eNOS
Campa et al., Inflamm Res 2005 (Inflammation) : Thus, poly I induced an increase in nuclear factor kappaB (NF-kappaB) transcriptional activity due to a long-term degradation of inhibitory NF-kappaB (IkappaB) beta while lipopolysaccharide (LPS) induced the degradation of both IkappaBalpha and IkappaBbeta ... Thus, poly I induced an increase in nuclear factor kappaB (NF-kappaB) transcriptional activity due to a long-term degradation of inhibitory NF-kappaB (IkappaB) beta while lipopolysaccharide (LPS) induced the degradation of both IkappaBalpha and IkappaBbeta ... Thus, poly I induced an increase in nuclear factor kappaB (NF-kappaB) transcriptional activity due to a long-term degradation of inhibitory NF-kappaB (IkappaB) beta while lipopolysaccharide (LPS) induced the degradation of both IkappaBalpha and IkappaBbeta
Lin et al., Anticancer Res 2005 (Stomach Neoplasms) : It was shown that ( - ) -Menthol inhibited the proliferation of the cells in a dose- and time dependent manner, inhibited topoisomerase I , IIa and IIbeta, but promoted the levels of NF-kappaB gene expression based on the Western blot and polymerase chain reaction ( PCR ) and cDNA microarray methods
Wieser et al., J Clin Endocrinol Metab 2005 (Endometriosis) : EMSAs showed that sulindac dramatically decreased NF-kappaB activation and diminished TNFalpha and IL-1beta induced NF-kappaB DNA binding activity
Kim et al., Proc Natl Acad Sci U S A 2005 (Dysentery, Bacillary) : Transfection experiments indicated that OspG can prevent phospho-IkappaBalpha degradation and NF-kappaB activation induced by TNF-alpha stimulation
Kunter et al., Kidney Int 2005 (Nephritis) : A20, but not A1, inhibited TNF induced NF-kappaB activation by preventing IkappaBalpha degradation, hence subsequent up-regulation of the proinflammatory molecules ICAM-1 and MCP-1 ... A20, but not A1, inhibited TNF induced NF-kappaB activation by preventing IkappaBalpha degradation, hence subsequent up-regulation of the proinflammatory molecules ICAM-1 and MCP-1
Covert et al., Science 2005 : LPS binding to Toll-like receptor 4 (TLR4) causes activation of NF-kappaB that requires two downstream pathways, each of which when isolated exhibits damped oscillatory behavior
Steinbrecher et al., Mol Cell Biol 2005 : Consistent with this result, cells from these mice have diminished nuclear factor kappaB (NF-kappaB) activity, implying a functional role for GSK-3beta in regulating NF-kappaB
Matsuyama et al., Am J Respir Cell Mol Biol 2005 (Sarcoidosis, Pulmonary) : DDR1 activation also induced NF-kappaB nuclear translocation in CD14 positive BALF cells of patients with deteriorated PS ... The inhibitor of NF-kappaB inhibited the production of MCP-1 and MMP-9 ... The inhibitor of NF-kappaB inhibited the production of MCP-1 and MMP-9
Yun et al., Toxicology 2006 : These results suggest that PhIP has potential immunosuppressive effects by inhibiting T-cell proliferation and IL-2 expression through down regulation of ROS generation and thereby inhibiting NF-kappaB , AP-1 and NF-AT activation ... These results suggest that PhIP has potential immunosuppressive effects by inhibiting T-cell proliferation and IL-2 expression through down regulation of ROS generation and thereby inhibiting NF-kappaB , AP-1 and NF-AT activation
Ogunwobi et al., Regul Pept 2006 (Colonic Neoplasms) : Activation of NF-kappaB was independent of ERK and p38 MAP kinase
Yemelyanov et al., Oncogene 2006 (Neoplasm Invasiveness...) : In this work, using luciferase assay, electrophoretic mobility shift assay and Northern blot analysis of expression of endogenous kappaB-responsive genes, we demonstrate that a novel highly specific small-molecule IKK inhibitor, PS1145, efficiently inhibited both basal and induced NF-kappaB activity in PC cells
Chen et al., Eur J Pharmacol 2005 : LPS induced IkappaB kinase (IKK), nuclear factor-kappaB (NF-kappaB) and activating protein-1 (AP-1) activation, and IFN-gamma induced NF-kappaB , signal transducer and activator of transcription-1 ( STAT1 ) and interferon regulatory factor-1 (IRF-1) activation were reduced by quercetin ... LPS induced IkappaB kinase (IKK), nuclear factor-kappaB (NF-kappaB) and activating protein-1 (AP-1) activation , and IFN-gamma induced NF-kappaB, signal transducer and activator of transcription-1 ( STAT1 ) and interferon regulatory factor-1 (IRF-1) activation were reduced by quercetin
Choi et al., J Neurosci Res 2005 (Alcohol-Induced Disorders, Nervous System...) : Lipopolysaccharide (LPS), gangliosides, and interferon (IFN)-gamma induced the inflammatory activation of glia, which was differentially influenced by ethanol : 1 ) ethanol inhibited LPS- or gangliosides induced, but not IFNgamma induced, glial activation as demonstrated by the production of nitric oxide and the expression of inflammatory genes such as interleukin-1beta, tumor necrosis factor-alpha, IP-10, and CD86 ; 2 ) nuclear factor (NF)-kappaB or JAK/STAT1 pathway was necessary for LPS- or IFNgamma induced glial activation, respectively ; 3 ) ethanol inhibited LPS induced NF-kappaB activation ; and 4 ) ethanol did not significantly affect IFNgamma induced STAT1/IRF-1 activation ... Lipopolysaccharide (LPS), gangliosides, and interferon (IFN)-gamma induced the inflammatory activation of glia, which was differentially influenced by ethanol : 1 ) ethanol inhibited LPS- or gangliosides induced, but not IFNgamma induced, glial activation as demonstrated by the production of nitric oxide and the expression of inflammatory genes such as interleukin-1beta, tumor necrosis factor-alpha, IP-10, and CD86 ; 2 ) nuclear factor (NF)-kappaB or JAK/STAT1 pathway was necessary for LPS- or IFNgamma induced glial activation, respectively ; 3 ) ethanol inhibited LPS induced NF-kappaB activation ; and 4 ) ethanol did not significantly affect IFNgamma induced STAT1/IRF-1 activation ... Lipopolysaccharide (LPS), gangliosides, and interferon (IFN)-gamma induced the inflammatory activation of glia, which was differentially influenced by ethanol : 1 ) ethanol inhibited LPS- or gangliosides induced, but not IFNgamma induced, glial activation as demonstrated by the production of nitric oxide and the expression of inflammatory genes such as interleukin-1beta, tumor necrosis factor-alpha, IP-10, and CD86 ; 2 ) nuclear factor (NF)-kappaB or JAK/STAT1 pathway was necessary for LPS- or IFNgamma induced glial activation, respectively ; 3 ) ethanol inhibited LPS induced NF-kappaB activation ; and 4 ) ethanol did not significantly affect IFNgamma induced STAT1/IRF-1 activation
Yozai et al., J Am Soc Nephrol 2005 (Albuminuria...) : MTX also reduced the high glucose induced NF-kappaB activation in vitro and in vivo
Nefedova et al., J Immunol 2005 : Inhibition of Jak2/STAT3 signaling resulted in activation of the transcription factor NF-kappaB ... Inhibition of Jak2/STAT3 signaling resulted in activation of the transcription factor NF-kappaB
Shimizu et al., J Immunol 2005 (Pneumonia, Mycoplasma) : Lipid associated membrane proteins from M. pneumoniae were found to induce NF-kappaB through TLR 2 in a human monocytic cell line, THP-1 ... The activation of NF-kappaB by the F0F1-ATPase was inhibited by a dominant negative construct of TLR1 and TLR6 ... These results indicate that the activation of NF-kappaB by F0F1-ATPase is dependent on TLR1, TLR2, and TLR6
Dias et al., J Nutr 2005 (Diabetes Mellitus, Experimental) : This study investigated the protective effects of quercetin treatment on oxidative stress, nuclear factor (NF)-kappaB activation and expression of inducible nitric oxide synthase (iNOS) in streptozotocin induced diabetic rats ... Eight weeks later we measured TBARS and hydroperoxide initiated chemiluminescence ( QL ) in liver as markers of oxidative stress, and activities of the antioxidant enzymes catalase, superoxide dismutase ( SOD ), and glutathione peroxidase, NF-kappaB activation by an electrophoretic mobility shift assay and expression of IkappaB kinases ( IKKalpha and IKKbeta ), the inhibitor IkappaB ( IkappaBalpha and IkappaBbeta ), and iNOS by Western blot ... Eight weeks later we measured TBARS and hydroperoxide initiated chemiluminescence ( QL ) in liver as markers of oxidative stress, and activities of the antioxidant enzymes catalase, superoxide dismutase ( SOD ), and glutathione peroxidase, NF-kappaB activation by an electrophoretic mobility shift assay and expression of IkappaB kinases ( IKKalpha and IKKbeta ), the inhibitor IkappaB ( IkappaBalpha and IkappaBbeta ), and iNOS by Western blot
Lee et al., Infect Immun 2005 : Our data indicate that the increased Ets/Elk and NF-kappaB promoter activities associated with M. smegmatis infected macrophages are responsible, at least in part, for the increased TNF-alpha and NOS2 production observed in these infected cells and that ERK1/2 is required for Ets/Elk activity and full TNF-alpha production ... Our data indicate that the increased Ets/Elk and NF-kappaB promoter activities associated with M. smegmatis infected macrophages are responsible, at least in part, for the increased TNF-alpha and NOS2 production observed in these infected cells and that ERK1/2 is required for Ets/Elk activity and full TNF-alpha production ... Our data indicate that the increased Ets/Elk and NF-kappaB promoter activities associated with M. smegmatis infected macrophages are responsible, at least in part, for the increased TNF-alpha and NOS2 production observed in these infected cells and that ERK1/2 is required for Ets/Elk activity and full TNF-alpha production ... Our data indicate that the increased Ets/Elk and NF-kappaB promoter activities associated with M. smegmatis infected macrophages are responsible , at least in part, for the increased TNF-alpha and NOS2 production observed in these infected cells and that ERK1/2 is required for Ets/Elk activity and full TNF-alpha production ... Our data indicate that the increased Ets/Elk and NF-kappaB promoter activities associated with M. smegmatis infected macrophages are responsible , at least in part, for the increased TNF-alpha and NOS2 production observed in these infected cells and that ERK1/2 is required for Ets/Elk activity and full TNF-alpha production
Meylan et al., Nature 2005 : Overexpression of Cardif results in interferon-beta and NF-kappaB promoter activation , and knockdown of Cardif by short interfering RNA inhibits RIG-I dependent antiviral responses
Nardelli et al., Endocrinology 2006 : Osteostat suppresses the early stage of osteoclastogenesis via inhibition of macrophage colony stimulating factor induced receptor activator of NF-kappaB ( RANK ) expression in the osteoclast precursor cells
Zeng et al., Am J Physiol Gastrointest Liver Physiol 2006 (Inflammation) : However, when NF-kappaB mediated or phosphatidylinositol 3-kinase/Akt proinflammatory signaling was blocked, flagellin could induce programmed cell death
Iwasaki et al., Curr Neurovasc Res 2005 (Hypertension, Pulmonary) : MCT treatment caused infiltration of inflammatory cells, fibrosis of the interstitium, and pulmonary arterial wall thickening with marked elevation of right ventricular ( RV ) pressure, which are characteristics of MCT induced PH. Gene expression of tumor necrosis factor-alpha (TNF-alpha) as well as DNA binding activity of nuclear factor-kappaB (NF-kappaB) increased at 1 week after MCT treatment, reached a maximum at 2 weeks, and then decreased to the pretreatment level at 3 weeks
Dhandapani et al., J Neurochem 2005 : Akt inhibition prevented NFkappaB binding, suggesting a role for Akt in SCF induced NFkappaB ... SCF up-regulated the anti-apoptotic genes, bcl-2 and bcl-xL in an NFkappaB dependent manner
Nakamichi et al., J Neurochem 2005 : Taken together, these results suggest that the recognition of dsRNA structure selectively induces the CXCL10 and CCL5 responses in microglia through vacuolar pH-dependent activation of NF-kappaB and MAPK signaling pathways ... Taken together, these results suggest that the recognition of dsRNA structure selectively induces the CXCL10 and CCL5 responses in microglia through vacuolar pH-dependent activation of NF-kappaB and MAPK signaling pathways
Tse et al., Biochem Pharmacol 2005 : Honokiol inhibits TNF-alpha stimulated NF-kappaB activation and NF-kappaB regulated gene expression through suppression of IKK activation ... We observed that the tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activation was blocked by honokiol in four different cancer cell lines as evidenced by EMSA
Chang et al., Microbes Infect 2006 : Flavivirus induces interferon-beta gene expression through a pathway involving RIG-I dependent IRF-3 and PI3K dependent NF-kappaB activation ... Flavivirus induces interferon-beta gene expression through a pathway involving RIG-I dependent IRF-3 and PI3K dependent NF-kappaB activation ... NF-kappaB activation is essential for IFN-beta induction in JEV- and DEN-2 infected cells ... Evidently, a cellular RNA helicase, retinoic acid-inducible gene I (RIG-I) , and a cellular kinase, phosphatidylinositol-3 kinase (PI3K), are required for flavivirus induced IRF-3 and NF-kappaB activation, respectively
Shin et al., Eur J Pharmacol 2005 : Finally, BMD compound could provide an invaluable tool to investigate NF-kappaB dependent iNOS expression, in addition to its therapeutic potential in NO-associated inflammatory diseases
Nys et al., Vascul Pharmacol 2005 (Lung Diseases) : Nitric oxide related products and myeloperoxidase in bronchoalveolar lavage fluids from patients with ALI activate NF-kappa B in alveolar cells and monocytes ... Furthermore, in vitro studies confirmed that NO* derived species ( peroxynitrite and nitrites ) and the neutrophil enzyme myeloperoxidase by themselves increased the activation of NF-kappaB , thereby arguing for an in vivo pathogenetic role of NO* related products and neutrophil enzymes to human ALI
Lee et al., J Pharmacol Exp Ther 2006 : Also, cardamomin inhibited not only LPS induced degradation and phosphorylation of inhibitor kappaBalpha ( IkappaBalpha ) but also activation of inhibitor kappaB ( IkappaB ) kinases and nuclear translocation of NF-kappaB
Bai et al., J Clin Invest 2005 (Arthritis...) : FHL2 inhibits TRAF6 induced NF-kappaB activity in wild-type osteoclast precursors and, in keeping with its role as a suppressor of TRAF6 mediated RANK signaling, TRAF6/RANK association is enhanced in FHL2-/- osteoclasts ... FHL2 inhibits TRAF6 induced NF-kappaB activity in wild-type osteoclast precursors and, in keeping with its role as a suppressor of TRAF6 mediated RANK signaling, TRAF6/RANK association is enhanced in FHL2-/- osteoclasts
Sato et al., Nat Immunol 2005 : TAK1-deficient cells failed to activate transcription factor NF-kappaB and mitogen activated protein kinases in response to interleukin 1beta, tumor necrosis factor and Toll-like receptor ligands ... TAK1-deficient cells failed to activate transcription factor NF-kappaB and mitogen activated protein kinases in response to interleukin 1beta , tumor necrosis factor and Toll-like receptor ligands
Sheu et al., Food Chem Toxicol 2006 (Glomerulonephritis) : Electrophoretic mobility shift assay experiments demonstrated that CLA ( 100 microM ) dramatically reduced activation of nuclear factor-kappaB (NF-kappaB) , activator protein-1 (AP-1) and cAMP response element binding protein ( CREB ) induced by LPS/IFN-gamma ... Electrophoretic mobility shift assay experiments demonstrated that CLA ( 100 microM ) dramatically reduced activation of nuclear factor-kappaB (NF-kappaB) , activator protein-1 (AP-1) and cAMP response element binding protein ( CREB ) induced by LPS/IFN-gamma
Martin et al., J Pharmacol Exp Ther 2006 : Activation of the A ( 3 ) AR or inhibition of intracellular Ca ( 2+ ) release also reduced LPS stimulated nuclear factor-kappaB (NF-kappaB) activation and extracellular signal regulated kinase 1/2 ( ERK1/2 ) phosphorylation
Tian et al., BMC genomics 2005 (Inflammation) : In response to TNF , latent cytoplasmic NF-kappaB is activated, enters the nucleus, and induces expression of inflammatory and anti-apoptotic gene expression programs
Jacque et al., Proc Natl Acad Sci U S A 2005 : In the absence of RelA phosphorylation on serine-276, TNF-alpha stimulation leads to a strong increase in the expression of endogenous NF-kappaB-responsive genes, such as Bcl-xL, whose transcriptional up-regulation is mainly controlled by RelB
Sawai et al., Med Sci Monit 2005 (Pancreatic Neoplasms) : EMSA confirmed that IL-1alpha increased DNA binding activity of AP-1 and NF-kappaB ... These results suggest that MAPK/AP-1 and ROS/NF-kappaB signaling pathways are involved in IL-1alpha induced IL-8 secretion and that these paracrine signaling pathways enhance endothelial cell proliferation
Gil et al., Virus Res 2006 : However, the NF-kappaB response to bacterial lipopolysaccarides (LPS) was normal in these cells, suggesting a specific suppression of antiviral response signaling in ncp BVDV infected cells
Kang et al., J Toxicol Environ Health 2005 : Furthermore, Src TK selective inhibitors-damnacanthal or PP1 blocked LPS induced NF-kappaB activation over a range of nanomolar concentrations ... In addition to serine phosphorylation of IkappaB-alpha, LPS induced NF-kappaB activation also required tyrosine phosphorylation of IkappaB-alpha
Saatian et al., Biochem J 2006 : Furthermore, SB203580 attenuated LPA dependent phosphorylation of IkappaB ( inhibitory kappaB ), NF-kappaB and phospho-p38 translocation to the nucleus, NF-kappaB transcription and IL-8 promoter mediated luciferase reporter activity, without affecting the JNK pathway and AP-1 transcription ... Similarly, JNK ( i ) II only blocked LPA mediated phosphorylation of JNK and c-Jun, AP-1 transcription and IL-8 promoter mediated luciferase reporter activity, without blocking p38 MAPK dependent NF-kappaB transcription
Wang et al., Allergy 2005 : Induction of IL-6 in co-culture of bronchial epithelial cells and eosinophils is regulated by p38 MAPK and NF-kappaB
Lim et al., FEBS Lett 2005 : The induction of COX-2 expression by hypertonic NaCl did not require the activation of NF-kappaB
Sankar et al., Cell Signal 2006 : Our data suggest that expression of IKK-i can activate both NFkappaB and IRF3, leading to the production of several cytokines including interferon beta
Kim et al., J Biol Chem 2005 (Colonic Neoplasms...) : Biochemical analysis revealed that ASA inhibited NF-kappaB activity, which is known to regulate BCL-2 gene expression, by dephosphorylating IkappaB-alpha and inhibiting IKKbeta activity but not by affecting the HER-2/neu phosphatidylinositol 3-kinase-Akt signal pathway ... Biochemical analysis revealed that ASA inhibited NF-kappaB activity, which is known to regulate BCL-2 gene expression, by dephosphorylating IkappaB-alpha and inhibiting IKKbeta activity but not by affecting the HER-2/neu phosphatidylinositol 3-kinase-Akt signal pathway
Hu et al., J Huazhong Univ Sci Technolog Med Sci 2005 : It was concluded that the expressions of NF-kappaB in hRPE cells could be increased significantly by IL-1beta and depressed effectively by PDTC ... Also, PDTC could significantly inhibit the activation of NF-kappaB induced by IL-1beta
Haneji et al., Nutr Cancer 2005 (Leukemia, T-Cell...) : Further analysis showed that fucoidan inactivated NF-kappaB and activator protein-1 and inhibited NF-kappaB-inducible chemokine, C-C chemokine ligand 5 ( regulated on activation, normal T expressed and secreted ) production, and homotypic cell-cell adhesion of HTLV-1 infected T-cell lines
Huang et al., J Ethnopharmacol 2006 : Furthermore, harpagoside dose-dependently inhibited LPS stimulated NF-kappaB promoter activity in a gene reporter assay in RAW 264.7 cells, indicating that harpagoside interfered with the activation of gene transcription ... These results suggest that the inhibition of the expression of cyclooxygenase-2 and inducible nitric oxide by harpagoside involves suppression of NF-kappaB activation, thereby inhibiting downstream inflammation and subsequent pain events
McDonald et al., J Biol Chem 2005 (Inflammation...) : A role for Erbin in the regulation of Nod2 dependent NF-kappaB signaling ... A role for Erbin in the regulation of Nod2 dependent NF-kappaB signaling ... We found that Nod2 dependent activation of NF-kappaB and cytokine secretion is inhibited by Erbin overexpression, whereas Erbin-/- mouse embryo fibroblasts show an increased sensitivity to muramyl dipeptide ... We found that Nod2 dependent activation of NF-kappaB and cytokine secretion is inhibited by Erbin overexpression, whereas Erbin-/- mouse embryo fibroblasts show an increased sensitivity to muramyl dipeptide
Williams et al., J Biol Chem 2005 (Inflammation) : Monarch-1 reduces NFkappaB activation by TLR signaling molecules MyD88, IRAK-1 ( type I interleukin-1 receptor associated protein kinase ), and TRAF6 ( TNF receptor (TNFR) associated factor ) as well as TNFR signaling molecules TRAF2 and RIP1 but not the downstream NFkappaB subunit p65 ... Reducing Monarch-1 expression with small interference RNA in myeloid/monocytic cells caused a dramatic increase in NFkappaB activation and cytokine expression in response to TLR2/TLR4 agonists, TNFalpha , or M. tuberculosis infection, suggesting that Monarch-1 is a negative regulator of inflammation ... Reducing Monarch-1 expression with small interference RNA in myeloid/monocytic cells caused a dramatic increase in NFkappaB activation and cytokine expression in response to TLR2/TLR4 agonists, TNFalpha, or M. tuberculosis infection, suggesting that Monarch-1 is a negative regulator of inflammation
Won et al., Biol Pharm Bull 2005 : Furthermore, chiisanoside inhibited the nuclear factor-kappaB (NF-kappaB) activation induced by LPS and this was associated with a reduction in p65 protein in the nucleus and with the phosphorylations of ERK1/2 and JNK MAP kinases
Kim et al., Dermatology 2005 (Acne Vulgaris) : Using transfectant cells we found that TLR2 was sufficient for NF-kappaB activation in response to P. acnes
Ueno et al., Shock 2005 (Body Weight...) : NFkappaB activation was increased in response to LPS in chow mice, whereas there was no increase in the IV-TPN group
Zhang et al., Alcohol Clin Exp Res 2005 (HIV Infections) : Alcohol inhibited IL-2 induced NF-kappaB p65 protein expression and calcium mobilization by NK cells ... Alcohol, through the inhibition of IL-2 induced NF-kappaB p65 protein expression and intracellular calcium mobilization, suppressed NK cell production of CC chemokines
Zheng et al., Virchows Arch 2006 (Lupus Nephritis...) : In contrast to non-proliferative glomerulopathy and normal controls, NF-kappaB activation ( both p65 and p50 ) was enhanced in glomerular endothelial, mesangial cells or infiltrating cells in class IV LN, along with upregulation of TNF-alpha, IL-1beta, IL-6 and ICAM-1 expression
Liu et al., Planta Med 2005 : Phthalide Lactones from Ligusticum chuanxiong inhibit lipopolysaccharide induced TNF-alpha production and TNF-alpha mediated NF-kappaB Activation ... Furthermore, the two phthalides exhibited significant suppressive effects on TNF-alpha mediated nuclear factor-kappaB (NF-kappaB) activation in reporter gene assays
Lawrence et al., Glia 2006 (AIDS Dementia Complex...) : Electromobility shift assays ( EMSAs ) demonstrated increased nuclear NF-kappaB levels within 2 h of initiating astrocyte differentiation, and inhibitors of NF-kappaB activation partially blocked the CCL2 increase in differentiating astrocytes ... Together these results suggest that the transcription factor NF-kappaB , and possibly NF-I, contribute to the upregulation of CCL2 chemokine production during the differentiation of human progenitor cells toward an astrocyte phenotype
Lövborg et al., Int J Cancer 2006 : As expected for proteasome inhibitors, these compounds also inhibited TNF-alpha induced nuclear factor-KappaB (NF-KappaB) translocation and were cytotoxic
Zhang et al., Journal of inflammation (London, England) 2005 : Regulation of IkappaBalpha expression involves both NF-kappaB and the MAP kinase signaling pathways ... Expression of IkappaBalpha itself is regulated by NF-kappaB , which provides auto-regulation of this signaling pathway ... Our data demonstrate that regulation of IkappaBalpha expression involves both the NF-kappaB and the p38 signaling pathways
Tanaka et al., J Biol Chem 2005 : 3-Phosphoinositide dependent protein kinase-1 mediated IkappaB kinase beta (IkkB) phosphorylation activates NF-kappaB signaling ... Here we show that PDK1 can activate IKK/NF-kappaB signaling in addition to Akt signaling to promote cell survival ... IKKalpha is not required for PDK1 mediated NF-kappaB activation because NF-kappaB activation was observed in IKKalpha ( -/- ) mouse embryonic fibroblast (MEF) cells as in wild type MEF cells ... IKKalpha is not required for PDK1 mediated NF-kappaB activation because NF-kappaB activation was observed in IKKalpha ( -/- ) mouse embryonic fibroblast (MEF) cells as in wild type MEF cells ... Akt, which was previously reported to activate IKKalpha, did not participate in the PDK1 dependent IKKbeta or NF-kappaB activation
Baouz et al., Int Immunol 2005 (Asthma) : Herein, we analysed the pharmacological response of lung myofibroblasts to beta2-agonists associated or not to inhaled corticosteroids, investigating their effects on ( i ) the constitutive and transforming growth factor-beta ( TGF-beta ) -induced expression of alpha-smooth muscle actin ( alpha-SMA ), the main functional marker of myofibroblastic differentiation and contractility ; ( ii ) isometric contraction and ( iii ) tumour necrosis factor-alpha (TNF-alpha) induced nuclear translocation of the pro-inflammatory transcription factor nuclear factor-kappaB (NF-kappaB) ... Second, the two drugs block the TNF-alpha induced nuclear translocation of the pro-inflammatory transcription factor NF-kappaB
Boonyaratanakornkit et al., FASEB J 2005 : Since NF-kappaB , AP-1, and CREB are all regulated by PKA and are transcription factors predicted by microarray analysis to be involved in the altered gene expression in vectorless gravity, the data suggest that PKA is a key player in the loss of T-cell activation in altered gravity
Lu et al., J Immunol 2005 : Thus, in the presence of LPS and H ( 2 ) O ( 2 ), NF-kappaB plays a dominate role in the regulation of MMP-1, COX-2, and PGE ( 2 ) expression ... Thus, in the presence of LPS and H ( 2 ) O ( 2 ), NF-kappaB plays a dominate role in the regulation of MMP-1 , COX-2, and PGE ( 2 ) expression ... Thus, in the presence of LPS and H ( 2 ) O ( 2 ), NF-kappaB plays a dominate role in the regulation of MMP-1, COX-2 , and PGE ( 2 ) expression
Matsumoto et al., Int J Oncol 2005 (Pancreatic Neoplasms) : NF-kappaB was activated by TNF-alpha ; however, the administration of DHMEQ abrogated its transcriptional activity
Tao et al., Cell Mol Immunol 2004 : In addition, RV reduced phosphorylation of extracellular signal regulated kinase ( ERK1/2 ) and the activation of nuclear factor NF-kappaB induced by formylpeptide receptor agonists
Gómez-Hernández et al., Atherosclerosis 2006 (Carotid Artery Diseases) : We have studied COX-2, mPGES-1 and PGE2 receptors ( EPs ) expression in peripheral blood mononuclear cells ( PBMC ) and atherosclerotic plaques of 29 patients with carotid stenosis as well as the effect of different nuclear factor-kappaB (NF-kappaB) inhibitors on COX-2, mPGES-1 and EPs expression in cultured monocytic cells ( THP-1 ) ... We have studied COX-2, mPGES-1 and PGE2 receptors ( EPs ) expression in peripheral blood mononuclear cells ( PBMC ) and atherosclerotic plaques of 29 patients with carotid stenosis as well as the effect of different nuclear factor-kappaB (NF-kappaB) inhibitors on COX-2, mPGES-1 and EPs expression in cultured monocytic cells ( THP-1 ) ... In cytokine treated cultured THP-1 , the NF-kappaB inhibitors parthenolide, Bay 11-7082 and PDTC reduced COX-2, mPGES-1 and EP-1/EP-3/EP-4 expression as well as PGE2 levels ... In cytokine treated cultured THP-1, the NF-kappaB inhibitors parthenolide, Bay 11-7082 and PDTC reduced COX-2, mPGES-1 and EP-1/EP-3/EP-4 expression as well as PGE2 levels ... In cytokine treated cultured THP-1, the NF-kappaB inhibitors parthenolide, Bay 11-7082 and PDTC reduced COX-2 , mPGES-1 and EP-1/EP-3/EP-4 expression as well as PGE2 levels ... We have studied COX-2, mPGES-1 and PGE2 receptors ( EPs ) expression in peripheral blood mononuclear cells ( PBMC ) and atherosclerotic plaques of 29 patients with carotid stenosis as well as the effect of different nuclear factor-kappaB (NF-kappaB) inhibitors on COX-2 , mPGES-1 and EPs expression in cultured monocytic cells ( THP-1 )
Tzimas et al., Cell Signal 2006 : Overexpression of USP31 in HEK 293T cells inhibited TNFalpha, CD40, LMP1, TRAF2, TRAF6 and IKKbeta mediated NF-kappaB activation, but did not inhibit Smad mediated transcription activation ... Overexpression of USP31 in HEK 293T cells inhibited TNFalpha, CD40, LMP1, TRAF2, TRAF6 and IKKbeta mediated NF-kappaB activation, but did not inhibit Smad mediated transcription activation ... Our data support a role for USP31 in the regulation of NF-kappaB activation by members of the TNF receptor superfamily
Jensen et al., J Endocrinol 2005 : Gel retardation experiments showed that hGH affects neither IFN-gamma+TNF-alpha induced STAT1 DNA binding nor IL-1beta and IFN-gamma+TNF-alpha induced NFkappaB DNA binding ... Gel retardation experiments showed that hGH affects neither IFN-gamma+TNF-alpha induced STAT1 DNA binding nor IL-1beta and IFN-gamma+TNF-alpha induced NFkappaB DNA binding
Schneeweis et al., J Biol Chem 2005 : The receptor activator of NF-kappaB ( RANK ) belongs to the neuregulin/tumor necrosis factor (TNF) receptor superfamily and is activated by RANK ligand (RANK-L) , a homotrimeric, TNF-like cytokine
Vandoros et al., J Cancer Res Clin Oncol 2006 (Adenocarcinoma...) : Induction of COX-2 expression is primarily induced by NF-kB
Deepak et al., Biochimie 2006 (Carcinoma, Ehrlich Tumor...) : Antiangiogenic and proapoptotic activity of a novel glycoprotein from U. indica is mediated by NF-kappaB and Caspase activated DNase in ascites tumor model
Aggarwal et al., Mol Pharmacol 2006 (Neoplasm Metastasis) : Screening of 20 different analogs of curcumin showed that curcumin was the most potent analog in suppressing the tumor necrosis factor (TNF) induced NF-kappaB activation ... Curcumin also suppressed NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)1 , TNFR2, NF-kappaB inducing kinase, IkappaB kinase complex (IKK), and the p65 subunit of NF-kappaB ... Curcumin also suppressed NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)1 , TNFR2, NF-kappaB inducing kinase, IkappaB kinase complex (IKK), and the p65 subunit of NF-kappaB ... Curcumin also suppressed NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)1, TNFR2 , NF-kappaB inducing kinase, IkappaB kinase complex (IKK), and the p65 subunit of NF-kappaB ... Curcumin also suppressed NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)1, TNFR2, NF-kappaB inducing kinase, IkappaB kinase complex (IKK) , and the p65 subunit of NF-kappaB ... Glutathione and dithiothreitol reversed the effect of curcumin on TNF induced NF-kappaB activation
Mashima et al., J Biol Chem 2005 : The forced expression of FLN29 in macrophage-like RAW cells resulted in the suppression of TLR mediated NF-kappaB and mitogen activated protein kinase activation, while a reduced expression of FLN29 by small interfering RNA partly cancelled the down-regulation of LPS signaling ... The forced expression of FLN29 in macrophage-like RAW cells resulted in the suppression of TLR mediated NF-kappaB and mitogen activated protein kinase activation , while a reduced expression of FLN29 by small interfering RNA partly cancelled the down-regulation of LPS signaling ... Furthermore, we demonstrated that NF-kappaB activation induced by TRAF6 and TAB2 was impaired by co-expression of FLN29, suggesting FLN29 may regulate the downstream of TRAF6 ... Furthermore, we demonstrated that NF-kappaB activation induced by TRAF6 and TAB2 was impaired by co-expression of FLN29, suggesting FLN29 may regulate the downstream of TRAF6
Hattori et al., Biochem Biophys Res Commun 2005 : The stimulation of neutrophil-like differentiated HL60 cells with TNF-alpha induced activation of NFkappaB and c-Src kinase, and the activation was attenuated by treatment with the anti-oxidants
Ross et al., Blood 2006 (Inflammation) : The alpha9beta1 signaling pathway shares significant features with the IFN-beta survival signaling pathway, requiring PI3 kinase, NF-kappaB activation , as well as de novo protein synthesis, but the kinetics of NF-kappaB activation by VCAM-1 were slower and more sustained compared with IFN-beta
Zhang et al., Circ Res 2005 : Activation of nuclear factor (NF)-kappaB by angiotensin II (Ang II) plays an essential role in stimulating expression of vascular adhesion molecules, which are essential for vascular inflammation
Okahira et al., DNA Cell Biol 2005 : Toll-like receptor (TLR) 3 , a member of TLR family proteins, recognizes extracellular dsRNA and activates NF- kappaB and the IFN-beta promoter leading to the induction of IFN-beta production
Ouyang et al., Cancer Res 2005 : The induction of cyclin D1 by arsenite required nuclear factor-kappaB (NF-kappaB) activation, because the inhibition of IkappaB phosphorylation by overexpression of the dominant negative mutant, IKKbeta-KM, impaired arsenite induced cyclin D1 expression and G1-S transition
Shishodia et al., Cancer Res 2005 (Carcinoma, Small Cell...) : We found that 4-HPR suppressed both inducible and constitutive NF-kappaB activation without interfering with the direct DNA binding of NF-kappaB ... NF-kappaB dependent reporter gene expression was also suppressed by 4-HPR, as was NF-kappaB reporter activity induced by TNFR1 , TRADD, TRAF2, NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by 4-HPR, as was NF-kappaB reporter activity induced by TNFR1, TRADD , TRAF2, NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by 4-HPR, as was NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2 , NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by 4-HPR, as was NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2 , NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by 4-HPR, as was NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2, NIK, and IKK but not that induced by p65 transfection
Hosokawa et al., Clin Exp Immunol 2005 : On the other hand, we found that not only NF-kappaB , p38 MAPK and ERK but also c-Jun NH2-terminal kinase (JNK) are involved in CCL20 production induced by E. coli LPS
Zhu et al., Immunol Lett 2006 : The results from the systems showed that all the three mAbs could neutralize TNF mediated cytotoxicity in L929 cells, TNF induced NF-kappaB activation in ECV304 cells, and TNF upregulated ICAM-1 surface expression on ECV304 cells in dose dependent manners
Recklies et al., J Biol Chem 2005 (Inflammation) : Thus, induction and continued secretion of CHI3L1 in chondrocytes require sustained activation of NF-kappaB
Rouquette-Jazdanian et al., J Immunol 2005 : Combined all together : raft modification in terms of lipids, ceramide production, protein kinase Calpha inhibition , and NF-kappaB activation lead to CD4+ T cell inhibition
Hsiung et al., J Neurochem 2005 : Stimulation of 5-HT 1A receptor also resulted in activation of NF-kappaB through a decrease in inhibitor of nuclear transcription factor-kappaB ... Stimulation of 5-HT 1A receptor also resulted in activation of NF-kappaB through a decrease in inhibitor of nuclear transcription factor-kappaB
Furuno et al., Neurosci Lett 2006 : Neurotrophic factors increase tumor necrosis factor-alpha induced nuclear translocation of NF-kappaB in rat PC12 cells ... Using green fluorescent protein tagged NF-kappaB1 ( GFP-NF-kappaB1 ), we examined here the effects of TNF-alpha and neurotrophic factors on the nuclear translocation of NF-kappaB in PC12 cells ... TNF-alpha induced gradually the translocation of GFP-NF-kappaB1 from the cytoplasm to the nucleus within 60 min. Pretreatment of lactacystin which is a proteasome-specific inhibitor suppressed significantly the nuclear translocation of GFP-NF-kappaB1 after TNF-alpha stimulation ... These results suggested that there is a close correlation between the signaling pathways via TNF receptors and neurotrophin receptors for the NF-kappaB activation, and that NGF and BDNF enhance TNF-alpha induced nuclear translocation of NF-kappaB ... These results suggested that there is a close correlation between the signaling pathways via TNF receptors and neurotrophin receptors for the NF-kappaB activation, and that NGF and BDNF enhance TNF-alpha induced nuclear translocation of NF-kappaB ... These results suggested that there is a close correlation between the signaling pathways via TNF receptors and neurotrophin receptors for the NF-kappaB activation, and that NGF and BDNF enhance TNF-alpha induced nuclear translocation of NF-kappaB
Kumar et al., Microbes Infect 2006 : The Pam3Cys induced hBD2 expression was completely blocked by NF-kappaB inhibitors and partially inhibited by p38 MAP kinase and the JNK inhibitors
Maeng et al., Cell Signal 2006 : Consistently, inhibition of ERK significantly increased IkappaB kinase (IKK) activity, IkappaBalpha phosphorylation, and nuclear translocation of NF-kappaB induced by VEGF, whereas overexpression of ERK resulted in the loss of these responses to VEGF ... Consistently, inhibition of ERK significantly increased IkappaB kinase (IKK) activity, IkappaBalpha phosphorylation, and nuclear translocation of NF-kappaB induced by VEGF , whereas overexpression of ERK resulted in the loss of these responses to VEGF
Sun et al., J Surg Res 2005 : In this study, we investigated the effects of HSP on LPS induced Kupffer cell NF-kappaB activation and cytokine production
Keller et al., Cell Biochem Biophys 2005 (Long QT Syndrome) : Expression of I593R HERG activates the unfolded protein response pathway and, separately, NF-kappaB signaling
Malladi et al., Inflammation 2004 : EPEC OMP up regulated iNOS, induced nitrite production and NF-kappaB and MAPK were activated in caco-2 cells ... Thus, EPEC OMPs induce iNOS expression and NO production through activation of NF-kappaB and MAPK
Devi et al., Eur J Pharmacol 2005 (Diabetes Mellitus, Experimental...) : Administration of 300 mg thioacetamide/kg to diabetic rats inhibited NF-kappaB regulated cyclin D1 signaling, explaining inhibited tissue repair, liver failure and death, whereas remarkably higher NF-kappaB-DNA binding but transient down regulation of cyclin D1 expression explains delayed tissue repair in the diabetic rats receiving 30 mg thioacetamide/kg
Pae et al., Atherosclerosis 2006 (Atherosclerosis) : Pre-treatment with HA inhibited the secretion of monocyte chemoattractant protein (MCP)-1, the expression of vascular cell adhesion molecule (VCAM)-1 and the activation of transcriptional nuclear factor (NF)-kappaB in HUVECs stimulated with tumor necrosis factor-alpha, the major pro-inflammatory cytokine causing endothelial inflammation ... Based on our findings, we suggest that Nrf2 dependent HO-1 expression induced by HA inhibits MCP-1 secretion, VCAM-1 expression and NF-kappaB activation associated with vascular injury and inflammation in atherosclerosis
Ma et al., J Biol Chem 2005 : These studies were performed to characterize the mechanisms by which Toll-like receptor 4 (TLR4) mediated NF-kappaB activation promotes resistance to cell death in macrophages ... When NF-kappaB activation was inhibited by a super-repressor, IkappaBalpha, the TLR4 ligand lipopolysaccharide induced the activation of caspase 8, the loss of mitochondrial transmembrane potential ( DeltaPsim ), and apoptotic cell death in macrophages
Chang et al., J Dent Res 2005 : NF-kappaB activation in human dental pulp stem cells by TNF and LPS ... NF-kappaB activation in human dental pulp stem cells by TNF and LPS ... Because the dental pulp is frequently infected by oral bacteria due to dental decay, in this study, we examined whether lipopolysaccharide (LPS) and tumor necrosis factor (TNF) activated the immunologic transcription factor nuclear factor kappa B (NF-kappaB) in DPSCs ... Because the dental pulp is frequently infected by oral bacteria due to dental decay, in this study, we examined whether lipopolysaccharide (LPS) and tumor necrosis factor (TNF) activated the immunologic transcription factor nuclear factor kappa B (NF-kappaB) in DPSCs ... Consistently, both TNF and LPS rapidly induced the expression of the NF-kappaB dependent gene interleukin-8 (IL-8)
Foster et al., J Dent Res 2005 : In parallel, we showed that VIP inhibits nuclear translocation of NFkappaB and c-Jun in a time dependent manner, but does not decrease the expression of CD14 receptors
Thornburg et al., Oncogene 2006 (Lymphoma) : LMP1 signaling and activation of NF-kappaB in LMP1 transgenic mice ... The LMP1 mediated activation of NF-kappaB may contribute to the specific activation of c-Rel and lead to the increased development of lymphoma in the LMP1 transgenic mice
Narumi et al., J Immunol 1992 : IFN-gamma and IL-2 cooperatively activate NF kappa B in murine peritoneal macrophages ... IFN-gamma and IL-2 cooperatively activate NF kappa B in murine peritoneal macrophages ... Although the effects of LPS and IFN-gamma/IL-2 on NF kappa B activity were both transient, response to LPS was detected earlier and declined more rapidly than that seen with IFN-gamma/IL-2 treatment ... Although the effects of LPS and IFN-gamma/IL-2 on NF kappa B activity were both transient, response to LPS was detected earlier and declined more rapidly than that seen with IFN-gamma/IL-2 treatment ... Although the effects of LPS and IFN-gamma/IL-2 on NF kappa B activity were both transient, response to LPS was detected earlier and declined more rapidly than that seen with IFN-gamma/IL-2 treatment ... The activation of NF kappa B by IFN-gamma/IL-2 did not depend upon protein synthesis ... The activation of NF kappa B by IFN-gamma/IL-2 did not depend upon protein synthesis
Ahn et al., Blood 2006 : Reduction of LIME expression by the introduction of siRNA resulted in the disruption of BCR mediated activation of MAPK, calcium flux, NF-AT, PI3K, and NF-kappaB
Chen et al., Invest Ophthalmol Vis Sci 2005 : DHA ( 22 : 6n3 ) inhibited cytokine induced CAM expression through suppression of NF-kappaB nuclear translocation and upstream I-kappaBalpha phosphorylation and degradation
Morlon et al., Hum Mol Genet 2005 (Ectodermal Dysplasia) : TAB2, TRAF6 and TAK1 are involved in NF-kappaB activation induced by the TNF-receptor , Edar and its adaptator Edaradd ... TAB2, TRAF6 and TAK1 are involved in NF-kappaB activation induced by the TNF-receptor, Edar and its adaptator Edaradd ... TAB2, TRAF6 and TAK1 are involved in NF-kappaB activation induced by the TNF-receptor, Edar and its adaptator Edaradd ... TAB2 , TRAF6 and TAK1 are involved in NF-kappaB activation induced by the TNF-receptor, Edar and its adaptator Edaradd ... TAB2, TRAF6 and TAK1 are involved in NF-kappaB activation induced by the TNF-receptor, Edar and its adaptator Edaradd ... TAB2, TRAF6 and TAK1 are involved in NF-kappaB activation induced by the TNF-receptor, Edar and its adaptator Edaradd ... TAB2, TRAF6 and TAK1 are involved in NF-kappaB activation induced by the TNF-receptor, Edar and its adaptator Edaradd ... Moreover, we show that dominant negative forms of TAB2, TRAF6 and TAK1 blocked the NF-kappaB activation induced by Edaradd ... Moreover, we show that dominant negative forms of TAB2 , TRAF6 and TAK1 blocked the NF-kappaB activation induced by Edaradd ... Moreover, we show that dominant negative forms of TAB2, TRAF6 and TAK1 blocked the NF-kappaB activation induced by Edaradd ... Moreover, we show that dominant negative forms of TAB2, TRAF6 and TAK1 blocked the NF-kappaB activation induced by Edaradd
Tseng et al., Am J Physiol Cell Physiol 2006 (Inflammation) : Apical Pseudomonas aeruginosa strain PAK ( but not flagellin mutant PAK.fliC ), flagellin, and flagellin + PAK.fliC activated NF-kappaB and IL-8 expression and secretion
Kang et al., Eur J Pharmacol 2005 : PGG treatment attenuated tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor-kappaB (NF-kappaB) p65 translocation in human umbilical vein endothelial cells
Frost et al., Am J Physiol Regul Integr Comp Physiol 2006 : Pam stimulated NF-kappaB and IL-6 promoter activities were disrupted by a dominant negative form of TLR2, but not TLR4
Saito et al., Arthritis Rheum 2005 (Chronic Disease...) : In addition, the Y570C mutation ( with or without the S196N polymorphism ) increased the ability of CIAS1 to induce ASC dependent NF-kappaB activation, unlike the wild-type gene or the gene bearing the S196N polymorphism alone ... The production of interleukin-1beta (IL-1beta) by peripheral blood mononuclear cells ( PBMCs ) was measured by enzyme linked immunosorbent assay, and the ability of the mutant CIAS1 gene to enhance ASC dependent NF-kappaB activation was assessed to confirm that the mutations of CIAS1 found were responsible for the patient 's clinical manifestations of the CINCA syndrome ... In addition, the Y570C mutation ( with or without the S196N polymorphism ) increased the ability of CIAS1 to induce ASC dependent NF-kappaB activation, unlike the wild-type gene or the gene bearing the S196N polymorphism alone
Stuhlmeier et al., J Biol Chem 2005 : Here we probe the involvement of the transcription factor NF-kappaB in induced and noninduced HAS activation
Son et al., Mol Cells 2005 : It also inhibited NF-IL6- and NF-kappaB induced activation of IL-1beta , with IC50 values of 78 nM and 1.6 microM, respectively, revealing a potent inhibitory effect on NF-IL6
Hua et al., Biochem Biophys Res Commun 2005 (Reperfusion Injury) : Transfection of Ad5-dnMyD88 significantly inhibited I/R enhanced NFkappaB activity by 50 % and increased the levels of phospho-Akt by 35.6 % and BCL-2 by 81 %, respectively
Eliseev et al., Exp Cell Res 2006 (Bone Neoplasms...) : Here, we show that in osteosarcoma Saos2 osteoblasts, NFkappaB regulates the activity of the BMP/Smad signaling ... Here, we show that in osteosarcoma Saos2 osteoblasts, NFkappaB regulates the activity of the BMP/Smad signaling ... We therefore conclude that in osteosarcoma Saos2 cells, NFkappaB represses BMP/Smad signaling and BMP2 induced differentiation through Smad7 ... We therefore conclude that in osteosarcoma Saos2 cells, NFkappaB represses BMP/Smad signaling and BMP2 induced differentiation through Smad7
Yamakuni et al., Neurosci Lett 2006 (Brain Neoplasms...) : In the course of our survey of natural compounds inhibiting prostaglandin E2 release and/or lipopolysaccharide (LPS) induced transcriptional stimulation via NF-kappaB, a central regulator of inflammatory genes, from natural resources, we found garcinone B, a xanthone from callus tissue culture of Hypericum patulum, as a compound with such pharmacological activities, that is a derivative of gamma-mangostin which potently inhibits COX-1 and COX-2 activities to reduce PGE2 release from C6 rat glioma cells, and inhibits IKK activity to prevent NF-kappaB dependent COX-2 gene transcription
Shim et al., Genes Dev 2005 : However, TAK1 is not required for NF-kappaB activation through the alternative pathway following LT-beta signaling ... However, TAK1 is not required for NF-kappaB activation through the alternative pathway following LT-beta signaling
Davies et al., Mol Cell Biol 2005 : In fibroblasts lacking TRAF2 or in carcinoma cells in which TRAF2 has been depleted by RNA interference, the CD40 mediated activation of NF-kappaB and JNK is significantly reduced, and the activation of p38 and Akt is severely impaired ... In fibroblasts lacking TRAF2 or in carcinoma cells in which TRAF2 has been depleted by RNA interference, the CD40 mediated activation of NF-kappaB and JNK is significantly reduced, and the activation of p38 and Akt is severely impaired ... In fibroblasts lacking TRAF2 or in carcinoma cells in which TRAF2 has been depleted by RNA interference, the CD40 mediated activation of NF-kappaB and JNK is significantly reduced, and the activation of p38 and Akt is severely impaired ... Interestingly, whereas the TRAF6 interacting membrane-proximal domain of CD40 has a minor role in signal transduction, studies utilizing TRAF6 knockout fibroblasts and RNA interference in epithelial cells reveal that the CD40 induced activation of NF-kappaB , JNK, p38, and Akt requires the integrity of TRAF6
Scian et al., Mol Cell Biol 2005 (Disease Progression) : Tumor derived p53 mutants induce NF-kappaB2 gene expression
Blonska et al., J Biol Chem 2005 : Receptor interacting protein ( RIP ) plays a critical role in tumor necrosis factor-alpha (TNF-alpha) induced IkappaB kinase (IKK) activation and subsequent activation of transcription factor NF-kappaB ... Receptor interacting protein ( RIP ) plays a critical role in tumor necrosis factor-alpha (TNF-alpha) induced IkappaB kinase (IKK) activation and subsequent activation of transcription factor NF-kappaB ... However, the molecular mechanism by which RIP mediates TNF-alpha induced NF-kappaB activation is not completely defined ... Moreover, a forced recruitment of TAK1 to TNF-R1 in the absence of RIP is sufficient to mediate TNF-alpha induced NF-kappaB activation, indicating that the major function of RIP is to recruit its downstream kinases to the TNF-R1 complex
Sugimori et al., J Bone Miner Metab 2005 : BMP-2 prevents apoptosis of the N1511 chondrocytic cell line through PI3K/Akt mediated NF-kappaB activation ... BMP-2 prevents apoptosis of the N1511 chondrocytic cell line through PI3K/Akt mediated NF-kappaB activation ... We investigated the involvement of phosphatidylinositol 3-kinase (PI3K)/Akt mediated NF-kappaB activation by BMP-2 stimulation in the modulation of this antiapoptotic process in a chondrocytic cell line, N1511 ... We investigated the involvement of phosphatidylinositol 3-kinase (PI3K)/Akt mediated NF-kappaB activation by BMP-2 stimulation in the modulation of this antiapoptotic process in a chondrocytic cell line, N1511 ... We investigated the involvement of phosphatidylinositol 3-kinase (PI3K)/Akt mediated NF-kappaB activation by BMP-2 stimulation in the modulation of this antiapoptotic process in a chondrocytic cell line, N1511 ... Thus, BMP-2 induced NF-kappaB activation is mediated by PI3K/Akt signaling ... Thus, BMP-2 induced NF-kappaB activation is mediated by PI3K/Akt signaling ... Thus, BMP-2 induced NF-kappaB activation is mediated by PI3K/Akt signaling
Pathak et al., J Biol Chem 2005 : It attenuates LPS induced NF-kappaB-driven luciferase gene expression, suggesting that its effects are likely directly related to inhibition of NF-kappaB
Gu et al., Nephrol Dial Transplant 2006 : NF-kappaB and Sp1 regulate MCP-1 gene transcription
Kim et al., Int Immunol 2005 (MAP Kinase Signaling System) : Moreover, inhibitors of MAPK ( U0126 and SB203580 ) significantly reduced the IL-8 production, while the inhibition of NF-kappaB ( pyrrolidinedithiocarbamate and retrovirus containing dominant negative IkappaB alpha plasmid ) did not affect the IL-8 expression increased by CpG ODN ... Moreover, inhibitors of MAPK ( U0126 and SB203580 ) significantly reduced the IL-8 production, while the inhibition of NF-kappaB ( pyrrolidinedithiocarbamate and retrovirus containing dominant negative IkappaB alpha plasmid ) did not affect the IL-8 expression increased by CpG ODN
Kukreja et al., Cancer Res 2005 (Bone Neoplasms...) : We tested the hypothesis that enhanced expression of CXCR4 in prostate cancer cells is dependent upon SDF-1alpha mediated activation of nuclear factor-kappaB (NF-kappaB) ... We tested the hypothesis that enhanced expression of CXCR4 in prostate cancer cells is dependent upon SDF-1alpha mediated activation of nuclear factor-kappaB (NF-kappaB) ... Suppression of NF-kappaB activity in PC-3 cells by a mutant IkappaBalpha super-repressor adenoviral vector decreased the CXCR4 mRNA expression and inhibited adhesion and TEM ... PD98059 cotreatment significantly inhibited SDF-1alpha induced NF-kappaB reporter activity and CXCR4 receptor expression as shown by flow cytometry ... These data suggest that SDF-1alpha induced expression of CXCR4 in PC-3 cells is dependent on MEK/ERK signaling cascade and NF-kappaB activation
Carter et al., J Biol Chem 2005 (Inflammation) : Nuclear translocation of NF-kappaB is regulated from the cytoplasm by IkappaB kinase-beta (IKKbeta) , which earmarks inhibitors of NF-kappaB for polyubiquination and proteasome mediated degradation
Choi et al., Mol Cells 2005 (MAP Kinase Signaling System) : PI3-kinase and PDK-1 regulate HDAC1 mediated transcriptional repression of transcription factor NF-kappaB ... PI3-kinase and PDK-1 regulate HDAC1 mediated transcriptional repression of transcription factor NF-kappaB ... Even though the crucial involvement of the PI3-kinase/Akt pathway in the anti-apoptotic activation of NF-kB is well known, the exact role of PDK-1 as well as PI3-kinase/Akt in NF-kB activation is not understood ... Even though the crucial involvement of the PI3-kinase/Akt pathway in the anti-apoptotic activation of NF-kB is well known, the exact role of PDK-1 as well as PI3-kinase/Akt in NF-kB activation is not understood ... Even though the crucial involvement of the PI3-kinase/Akt pathway in the anti-apoptotic activation of NF-kB is well known, the exact role of PDK-1 as well as PI3-kinase/Akt in NF-kB activation is not understood ... Here we demonstrate that PDK-1 plays a pivotal role in transcriptional activation of NF-kB by dissociating the transcriptional co-repressor HDAC1 from the p65 subunit of NF-kB ... Etoposide activated NF-kB through PI3-kinase/Akt, and the transcription activation domain ( TAD ) of p65 was further activated by wild-type PDK-1 ... Overexpression of a dominant negative PDK-1 mutant decreased etoposide induced NF-kB transcription and further down-regulated the ectopic HDAC1 mediated decrease in NF-kB transcriptional activity ... Overexpression of a dominant negative PDK-1 mutant decreased etoposide induced NF-kB transcription and further down-regulated the ectopic HDAC1 mediated decrease in NF-kB transcriptional activity ... Thus activation of PDK-1 relieves the HDAC1 mediated repression of NF-kB that may be related to basal as well as activated transcription by NF-kB
Bubici et al., Histol Histopathol 2006 (MAP Kinase Signaling System) : With regard to TNFalpha, the NF-kappaB mediated inhibition of PCD seems to involve an attenuation of the c-Jun-N-terminal kinase (JNK) cascade mediated through the induction of select downstream targets such as the caspase inhibitor XIAP, the zinc-finger protein A20, and the inhibitor of the MKK7/JNKK2 kinase, Gadd45beta/Myd118
Moon et al., Life Sci 2006 : AR treatment attenuated TNF-alpha induced NF-kappaB p65 translocation in HUVECs in a dose dependent manner
Liu et al., Mol Pharmacol 2006 (Lung Neoplasms...) : Exogenous supply of alpha-MSH inhibited NFkappaB activities, whereas application of the alpha-MSH antagonist growth hormone releasing peptide-6 ( GHRP-6 ) abolished the POMC induced inhibition of NFkappaB activities and melanoma growth in mice
Kobashi et al., Circ Res 2005 (Atherosclerosis) : We conclude that inhibition of NF-kappaB and activation of Akt phosphorylation may mediate adiponectin inhibition of atherosclerosis
Ponnappan et al., Immunity & ageing : I & A 2005 : Activation induced induction of transcription factor NFkappaB in T lymphocytes is regulated by its inhibitor IkappaBalpha
Broderick et al., Clin Immunol 2006 (Carcinoma, Non-Small-Cell Lung...) : Little translocation of NF-kappaB or NFAT occurred in tumor associated T cells in response to CD3+CD28 cross linking under conditions which led to maximal translocation in normal donor peripheral blood T cells ... TNF-alpha induced maximal NF-kappaB translocation in these T cells, indicating that they remain receptive to alternative signaling pathways, and pulsing with IL-12 prior to TCR triggering reversed their apparent anergy
Tsou et al., Toxicol Appl Pharmacol 2005 : Moreover, we found that depletion of GSH would also attenuate the TNF-alpha induced VCAM-1 expression with a down-regulation of the TNF-alpha induced NF-kappaB activation and without significant effect on AP-1 ... On the other hand, the TNF-alpha induced VCAM-1 expression could be completely abolished by inhibition of AP-1 or NF-kappaB activity, suggesting that activation of both AP-1 and NF-kappaB was necessary for VCAM-1 expression
Mandal et al., J Immunol 2005 (Inflammation) : Most interestingly, an anti-inflammatory PG ( PGA1 ) suppresses NF-kappaB activation and inhibits COX-2 gene expression ... Most interestingly, an anti-inflammatory PG ( PGA1 ) suppresses NF-kappaB activation and inhibits COX-2 gene expression
Gomez et al., J Immunol 2005 (Arthritis, Rheumatoid...) : In contrast, depletion of endogenous PGE2 by ibuprofen ( 100 microM ) and celecoxib ( 5 microM ) enhanced p65 , but inhibited p50 nuclear translocation as well as binding to NF-kappaB DNA binding sites
Yanaka et al., Int J Mol Med 2005 : Vitamin B6 inhibited LPS induced nuclear translocation of the NF-kappaB , the proinflammatory transcription factor, with reduction of the extent of LPS induced IkappaBalpha degradation in RAW cells
Kelly et al., Peptides 2006 : Peptide functionalized beads were compared with the ability of soluble peptide alone ( alpha-MSH or GKPV ) or non functionalized beads to inhibit TNF-alpha stimulated activation of NF-kappaB
Hill et al., Peptides 2006 : The present study confirms MC1R expression in cultured human dermal fibroblasts and reports that the MSH peptides alpha-MSH and KP ( -D- ) V inhibit TNF-alpha stimulated NF-kappaB activity and ICAM-1 upregulation, consistent with an anti-inflammatory role
Kanda et al., J Allergy Clin Immunol 2005 : CCL27 production by keratinocytes is dependent on nuclear factor kappaB (NF-kappaB) activity and enhanced in lesions of patients with atopic dermatitis, psoriasis vulgaris, or allergic contact dermatitis ... IL-17 suppressed TNF-alpha induced CCL27 secretion and mRNA expression and NF-kappaB activity in keratinocytes ... IL-17 suppressed TNF-alpha induced CCL27 secretion and mRNA expression and NF-kappaB activity in keratinocytes
Hseu et al., Int Immunopharmacol 2005 : These results indicate that A. camphorata inhibits LPS induction of cytokine, iNOS and COX-2 expression by blocking NF-kappaB activation
Szperka et al., Cytogenet Genome Res 2006 : FLICE-like inhibitory protein (FLIP) has been shown in both humans and mice to inhibit apoptosis and NF-kappaB activation induced by pro-inflammatory mediators ... Only the full-length bovine FLIP protein could inhibit NF-kappaB activation induced by LPS, whereas the death effector domain region alone was able to inhibit TNF-alpha induced NF-kappaB activation ... Only the full-length bovine FLIP protein could inhibit NF-kappaB activation induced by LPS , whereas the death effector domain region alone was able to inhibit TNF-alpha induced NF-kappaB activation
Thomas et al., Immunol Cell Biol 2006 : We found that NIT-1 cells are defective in the activation of nuclear factor-kappaB (NFkappaB) as a result of functionally deficient RelA activity, because overexpression of RelA protected NIT-1 cells from apoptosis
Dulos et al., Arthritis Res Ther 2005 (Arthritis, Rheumatoid) : In addition, inhibition of Cyp7b mRNA expression and activity was observed with SN50, PSI and SP600125, suggesting that NF-kappaB and AP-1 induce Cyp7b transcription
Luo et al., Cell Signal 2006 (MAP Kinase Signaling System) : LPS induced cPLA2 expression and PGE2 synthesis was inhibited by a selective NF-kappaB inhibitor ( helenalin ) and transfection with dominant negative mutants of NF-kappaB inducing kinase (NIK), IkappaB kinase (IKK)-alpha, and IKK-beta, consistent with that LPS stimulated both IkappaB-alpha degradation and NF-kappaB translocation into nucleus in these cells
Swiatkowska et al., FEBS J 2005 : We show that activation of PAI-1 gene by TNFalpha and reactive oxygen species is mediated by interaction of NF-kappaB with the cis acting element located in the -675 4G/5G insertion/deletion in the PAI-1 promoter ... We show that activation of PAI-1 gene by TNFalpha and reactive oxygen species is mediated by interaction of NF-kappaB with the cis acting element located in the -675 4G/5G insertion/deletion in the PAI-1 promoter
Wu et al., J Biol Chem 2006 (Cell Transformation, Neoplastic) : Although the CTAR1 mediated NF-kappaB activation was previously shown to be TRAF3 dependent, we showed here that the CTAR2 mediated NF-kappaB activation is mainly TRAF6 dependent but TRAF2/5 independent ... Furthermore, we showed that TAK1 is required for NF-kappaB activation by LMP1 ... Furthermore, we showed that TAK1 is required for NF-kappaB activation by LMP1
Samuel et al., J Biol Chem 2006 : Because TRAF2 regulates NF-kappaB activation, the effects of cIAP1 on TRAF2 mediated induction of NF-kappaB transcriptional activity were studied using reporter gene assays ... Because TRAF2 regulates NF-kappaB activation, the effects of cIAP1 on TRAF2 mediated induction of NF-kappaB transcriptional activity were studied using reporter gene assays ... Expression of a fragment of cIAP1 encompassing the three BIR domains ( but not full-length cIAP1 ) greatly enhanced TRAF2 induced increases in NF-kappaB activity, providing a convenient assay for monitoring BIR dependent effects of cIAP1 on TRAF2 in cells
Liu et al., J Virol 2005 : Akt/protein kinase B activation by adenovirus vectors contributes to NFkappaB dependent CXCL10 expression ... These results show that Akt plays a role in the Ad vector activation of NFkappaB and CXCL10 expression
Larsen et al., Diabetologia 2005 : However, inhibition of ERK reduced NFkappaB mediated Nos2 expression ; serine 276 phosphorylation of the p65 unit of the NFkappaB complex seemed critical, as evaluated by amino acid mutation analysis ... ERK activity is required for NFkappaB mediated transcription of Nos2 in insulin producing INS-1E cells, indicating that ERK regulates Nos2 expression by increasing the transactivating capacity of NFkappaB ... ERK activity is required for NFkappaB mediated transcription of Nos2 in insulin producing INS-1E cells, indicating that ERK regulates Nos2 expression by increasing the transactivating capacity of NFkappaB
De Stefano et al., J Mol Med (Berl) 2006 (Celiac Disease) : The role of NF-kappaB , IRF-1, and STAT-1alpha transcription factors in the iNOS gene induction by gliadin and IFN-gamma in RAW 264.7 macrophages
Liu et al., J Biol Chem 2006 : Phosphorylation of IkappaB-beta enhances its association with p65/RelA causing an increase in NF-kappaB DNA binding activity ... Phosphorylation of IkappaB-beta enhances its association with p65/RelA causing an increase in NF-kappaB DNA binding activity
Li et al., J Biol Chem 2006 (Breast Neoplasms) : Interleukin-1beta induction of NFkappaB is partially regulated by H2O2 mediated activation of NFkappaB inducing kinase ... In the present report, we describe a mechanism whereby interleukin-1beta (IL-1beta) stimulation of NFkappaB is partially regulated by H2O2 mediated activation of NIK and subsequent NIK mediated phosphorylation of IKKalpha ... IL-1beta induced H2O2 production in MCF-7 cells and clearance of this ROS through the expression of GPx-1 reduced NFkappaB transcriptional activation by inhibiting NIK mediated phosphorylation of IKKalpha ... Although IKKalpha and IKKbeta were both involved in IL-1beta mediated activation of NFkappaB , only the IKKalpha dependent component was modulated by changes in H2O2 levels ... Although IKKalpha and IKKbeta were both involved in IL-1beta mediated activation of NFkappaB , only the IKKalpha dependent component was modulated by changes in H2O2 levels ... Although IKKalpha and IKKbeta were both involved in IL-1beta mediated activation of NFkappaB , only the IKKalpha dependent component was modulated by changes in H2O2 levels ... In summary, our studies have demonstrated that redox regulation of NIK by H2O2 is mechanistically important in IL-1beta induction of NFkappaB activation
Ishii et al., Nat Immunol 2006 (Virus Diseases) : IPS-1 , an adaptor molecule linking RIG-I and TBK1, was involved in B-DNA induced activation of interferon-beta and NF-kappaB
Gurova et al., Proc Natl Acad Sci U S A 2005 (Carcinoma, Renal Cell) : Induction of p53 by these compounds does not involve genotoxic stress and is mediated by suppression of NF-kappaB activity ... The results demonstrate, in principle, the possibility to kill cancer cells selectively through simultaneous inhibition of NF-kappaB and activation of p53 by a single small molecule and suggest anticancer applications for the well known antimalaria drug quinacrine
Jiang et al., Proc Natl Acad Sci U S A 2005 (Hepatitis) : Poly I:C pretreatment also impaired the signaling of mitogen activated protein kinases and NF-kappaB induced by LPS in RAW264.7 cells
Lee et al., J Cell Physiol 2006 : Here, the roles of mitogen activated protein kinases ( MAPKs ) and NF-kappaB in TNF-alpha induced expression of vascular cell adhesion molecule (VCAM)-1 were investigated in human tracheal smooth muscle cells ( HTSMCs ) ... TNF-alpha stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha was blocked by helenalin, but not by U0126, SB202190, or SP600125 ... These results suggest that in HTSMCs, activation of MAPK pathways, NF-kappaB , and p300 is essential for TNF-alpha induced VCAM-1 expression
Ko et al., FEBS Lett 2005 : Recently, we reported that PAF upregulates MMP-9 expression in a NF-kappaB dependent manner ... Furthermore, PAF induced NF-kappaB activation was blocked by selective inhibitors of Ca ( 2+ ), PI3K , or extracellular signal regulated kinase ( ERK ) ... Furthermore, PAF induced NF-kappaB activation was blocked by selective inhibitors of Ca ( 2+ ), PI3K , or extracellular signal regulated kinase ( ERK ) ... Furthermore, PAF induced NF-kappaB activation was blocked by selective inhibitors of Ca ( 2+ ), PI3K, or extracellular signal regulated kinase ( ERK ) ... Our results suggest that PAF induced MMP-9 expression, in a NF-kappaB dependent manner, is regulated by Ca ( 2+ ), PI3K and ERK signaling pathways ... Our results suggest that PAF induced MMP-9 expression, in a NF-kappaB dependent manner, is regulated by Ca ( 2+ ), PI3K and ERK signaling pathways ... Our results suggest that PAF induced MMP-9 expression, in a NF-kappaB dependent manner, is regulated by Ca ( 2+ ), PI3K and ERK signaling pathways
Grau et al., Cell Signal 2006 (Colonic Neoplasms) : Role of Smad proteins in the regulation of NF-kappaB by TGF-beta in colon cancer cells ... Activation of the TGF-beta type I receptor is required for the TGF-beta mediated activation of NF-kappaB ... Smad4 restores the TGF-beta-1 mediated NF-kappaB activation in SW480 cells ... Smad4 restores the TGF-beta-1 mediated NF-kappaB activation in SW480 cells ... Taken together, these results suggest a role for Smad4 in the transcriptional activation of NF-kappaB , and a direct effect of Smad 7 inhibiting IkappaB-alpha phosphorylation rather than through the well established inhibition of Smad2/3 phosphorylation with subsequent inhibition of the TGF-beta pathway
Kang et al., Biochem Pharmacol 2005 : These results suggest that the inhibitory effect of equol on TNF-alpha expression is mediated , at least in part, by blocking NF-kappaB activation and the inhibition of TNF-alpha expression by equol might be involved in its osteoprotective effect
Abujamra et al., Virology 2006 : Using Toll-like receptor ( TLR ) deficient HEK293 cells and PKR ( -/- ) mouse embryo fibroblasts, we further demonstrate that although dsRNA activated protein kinase R (PKR) is not necessary, TLR3 is required for the activation of NFkappaB by the LTR
Zhou et al., Biochim Biophys Acta 2006 : A gel shift assay further showed that TNF-alpha induced Nuclear Factor-kappaB (NF-kappaB) activity was significantly reduced by cryptotanshinone
Liu et al., Cytokine 2005 : MEK1/2 and nuclear factor kappa B (NF-kappaB) inhibitors ( U0126 and TLCK, respectively ) blocked the ability of b-FGF to induce NRP-1 mRNA expression, but inhibition of JNK ( SP600125 ) or PI3-kinase activity ( wortmannin ) did not
Adam et al., J Biol Chem 2006 (Calcium Signaling...) : Second, Der p 1-mediated IL-8 expression was mainly dependent on NF-kappaB , whereas Der p 3 and PAR(2)AP regulated IL-8 expression through the activation of both NF-kappaB and AP-1
Masamune et al., J Cell Biochem 2006 : Curcumin inhibited interleukin-1beta- and TNF-alpha induced activation of activator protein-1 (AP-1) and mitogen activated protein ( MAP ) kinases ( ERK, c-Jun N-terminal kinase (JNK), and p38 MAP kinase ), but not of nuclear factor-kappaB (NF-kappaB) ... Curcumin inhibited interleukin-1beta- and TNF-alpha induced activation of activator protein-1 (AP-1) and mitogen activated protein ( MAP ) kinases ( ERK, c-Jun N-terminal kinase (JNK), and p38 MAP kinase ), but not of nuclear factor-kappaB (NF-kappaB)
Mao et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2005 (Brain Edema...) : The mechanism might be associated with its inhibitory effect on degradation of I-Kappa B alpha and activation of NF-KappaB
Wang et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2005 (Disease Models, Animal...) : NF-KappaB binding activity was inhibited at 2 and 5 hours of moderate hypothermia in lung tissue ( P < 0.01 ), but there was no difference in pancreatic tissue between normothermic and hypothermic rats at each time point
Fernández et al., Comp Biochem Physiol C Toxicol Pharmacol 2006 : In conclusion, T ( 3 ) -induced oxidative stress in the liver enhances the DNA binding of NF-kappaB and the NF-kappaB dependent expression of cytokines and iNOS by actions primarily exerted at the Kupffer cell level
Morante et al., Free radical research 2005 (Body Weight...) : Moreover, chromatin immunoprecipitation ( ChIP ) assay demonstrated that NF-kappaB directly regulates transcription of gamma-GCS ( both subunits ) and cyclin D1 through the binding of NF-kappaB to the corresponding gene promoters, which was enhanced in vitamin E-deficiency ... Moreover, chromatin immunoprecipitation ( ChIP ) assay demonstrated that NF-kappaB directly regulates transcription of gamma-GCS ( both subunits ) and cyclin D1 through the binding of NF-kappaB to the corresponding gene promoters, which was enhanced in vitamin E-deficiency
Mackenzie et al., Free radical research 2006 (Neuroblastoma) : Consistent with this, LA and NAC prevented zinc deficiency induced activation of the early steps of NF- kappaB ( IkappaBalpha phosphorylation ) and AP-1 [ c-Jun-N-terminal kinase (JNK) and p38 phophorylation ] cascades, and the high NF-kappaB- and AP-1-DNA binding activities in total cell extracts
Balasubramanian et al., J Gen Virol 2005 : Using specific NF-kappaB inhibitors, it was also shown that HCV-LP induced NF-kappaB activity mediated IL8 production in HUVECs
Schmeck et al., Am J Physiol Lung Cell Mol Physiol 2006 : Pneumococci induced TLR- and Rac1 dependent NF-kappaB-recruitment to the IL-8 promoter in lung epithelial cells ... In summary, these results suggest that S. pneumoniae activate human epithelial cells by TLR1/2 and a phosphatidylinositol 3-kinase- and Rac1 dependent NF-kappaB-recruitment to the IL-8 promoter
Chen et al., J Immunol 2005 : Moreover, C. parvum selectively up-regulated human beta-defensin-2 in directly infected cells, and inhibition of TLR2 and TLR4 signals or NF-kappaB activation were each associated with a reduction of C. parvum induced human beta-defensin-2 expression ... Moreover, C. parvum selectively up-regulated human beta-defensin-2 in directly infected cells, and inhibition of TLR2 and TLR4 signals or NF-kappaB activation were each associated with a reduction of C. parvum induced human beta-defensin-2 expression
Jeyaseelan et al., J Immunol 2005 (Escherichia coli Infections...) : LPS induced neutrophil sequestration ; NF-kappaB translocation ; keratinocyte cell derived chemokine, MIP-2, TNF-alpha, and IL-6 expression ; histopathology ; and VCAM-1 and ICAM-1 expression were abolished in the lungs of TIRAP-/- mice
Jones et al., J Immunol 2005 (Pneumonia, Pneumococcal) : Lung NF-kappaB activation and neutrophil recruitment require IL-1 and TNF receptor signaling during pneumococcal pneumonia ... Lung NF-kappaB activation and neutrophil recruitment require IL-1 and TNF receptor signaling during pneumococcal pneumonia
Iversen et al., J Immunol 2005 : Activated NK cells expressed lymphotoxin ( LT ) alphabeta on their cell surface, and secreted LTalpha and TNF, all of which contributed to the NF-kappaB dependent release of IFN-beta from infected fibroblasts
Carroll et al., J Immunol 2005 (Inflammation) : In contrast, only MyD88delta was found to inhibit IL-1 induced NF-kappaB reporter activity ... In contrast, only MyD88delta was found to inhibit IL-1 induced NF-kappaB reporter activity
Takeyama et al., Prostate 2006 (Inflammation) : Transient transfection of the dominant negative mutant TLR2 ( P681H ) into PC-3 cells attenuated M. hominis induced IL-8 secretion and NF-kappaB activation
Kobayashi et al., J Periodontal Res 2005 (MAP Kinase Signaling System) : Here, we show ( i ) a soybean KTI abrogates LPS induced up-regulation of TNF-alpha mRNA and protein expression in a dose dependent manner in gingival fibroblasts, ( ii ) KTI also blocks the induction of TNF-alpha target molecules interleukin-1beta (IL-1beta) and IL-6 proteins, ( iii ) inhibition by KTI of TNF-alpha induction correlates with the suppressive capacity of extracellular signal regulated kinase 1/2 ( ERK1/2 ) and p38 signaling pathways, implicating repressed ERK1/2 and p38 signalings in the inhibition, and ( iv ) pretreatment of cells with KTI blocked LPS induced nuclear factor kappaB (NFkappaB) activation ... Here, we show ( i ) a soybean KTI abrogates LPS induced up-regulation of TNF-alpha mRNA and protein expression in a dose dependent manner in gingival fibroblasts, ( ii ) KTI also blocks the induction of TNF-alpha target molecules interleukin-1beta (IL-1beta) and IL-6 proteins, ( iii ) inhibition by KTI of TNF-alpha induction correlates with the suppressive capacity of extracellular signal regulated kinase 1/2 ( ERK1/2 ) and p38 signaling pathways, implicating repressed ERK1/2 and p38 signalings in the inhibition, and ( iv ) pretreatment of cells with KTI blocked LPS induced nuclear factor kappaB (NFkappaB) activation
Gao et al., Cell communication and signaling : CCS 2005 : Activation of nuclear factor kappa B (NF-kappaB) by connective tissue growth factor ( CCN2 ) is involved in sustaining the survival of primary rat hepatic stellate cells
Migita et al., Life Sci 2006 : Further, TNF-alpha stimulation induced the degradation of IkappaB-alpha and resulted in the transcriptional activation of NF-kappaB
Poppema et al., Hematology / the Education Program of the American Society of Hematology. American Society of Hematology. Education Program 2005 (Hodgkin Disease) : A key role is played by the presence of constitutive nuclear factor (NF)-kappaB , which is induced by LMP1, as well as by CD30, CD40, tumor necrosis factor (TNF)-alpha, and Notch1 interactions, and results in the upregulation of at least 45 genes including chemokines, cytokines, receptors, apoptotic regulators, intracellular signaling molecules, and transcription factors ... A key role is played by the presence of constitutive nuclear factor (NF)-kappaB , which is induced by LMP1, as well as by CD30, CD40 , tumor necrosis factor (TNF)-alpha, and Notch1 interactions, and results in the upregulation of at least 45 genes including chemokines, cytokines, receptors, apoptotic regulators, intracellular signaling molecules, and transcription factors ... A key role is played by the presence of constitutive nuclear factor (NF)-kappaB , which is induced by LMP1, as well as by CD30 , CD40, tumor necrosis factor (TNF)-alpha, and Notch1 interactions, and results in the upregulation of at least 45 genes including chemokines, cytokines, receptors, apoptotic regulators, intracellular signaling molecules, and transcription factors ... A key role is played by the presence of constitutive nuclear factor (NF)-kappaB , which is induced by LMP1 , as well as by CD30, CD40, tumor necrosis factor (TNF)-alpha, and Notch1 interactions, and results in the upregulation of at least 45 genes including chemokines, cytokines, receptors, apoptotic regulators, intracellular signaling molecules, and transcription factors ... A key role is played by the presence of constitutive nuclear factor (NF)-kappaB , which is induced by LMP1, as well as by CD30, CD40, tumor necrosis factor (TNF)-alpha, and Notch1 interactions, and results in the upregulation of at least 45 genes including chemokines, cytokines, receptors, apoptotic regulators, intracellular signaling molecules, and transcription factors
Conti et al., J Neurosurg 2005 (Brain Neoplasms...) : The authors investigated the expression of NF-kappaB , caspase 3, TRAF1, TRAF2, and TRAF associated NF-kappaB activator/TRAF interacting protein ( TANK/I-TRAF ), a regulator of TRAF activity, in human gliomas
Morelock et al., Assay Drug Dev Technol 2005 : The NF kappaB antagonist assay ( inhibition of IL-1- induced NF kappaB translocation ) data were collected on a Q3DM ( San Diego, CA ) EIDAQtrade mark 100 high throughput microscopy system
Lin et al., J Biol Chem 2006 : In the present study, we demonstrate that the NF-kappaB-inducible, anti-apoptotic protein A20 efficiently blocks RIG-I mediated activation of NF-kappaB- , IRF-3-, and IRF-7 dependent promoters but only weakly interferes with TRIF-TLR-3 mediated IFN activation
Hou et al., J Biomed Sci 2006 (Brain Ischemia) : BT significantly reduced LPS induced NF-kappaB and p38 MAPK activation
Jung et al., Mech Ageing Dev 2006 (Inflammation) : NF-kappaB plays a key role in pro-inflammatory gene expression, such as cyclooxygenase (COX)
Zhuang et al., Cell Signal 2006 : Drosophila TAB2 is required for the immune activation of JNK and NF-kappaB
Guasparri et al., EMBO Rep 2006 : TRAF2 and TRAF3 are required for induction of NF-kappaB and associated cell survival, as well as Jun amino-terminal kinase phosphorylation by vFLIP, whereas TRAF1, TRAF5 and TRAF6 are dispensable ... TRAF2 and TRAF3 are required for induction of NF-kappaB and associated cell survival, as well as Jun amino-terminal kinase phosphorylation by vFLIP, whereas TRAF1, TRAF5 and TRAF6 are dispensable
Tsujita et al., Vaccine 2006 : These results suggest that the soluble TLR5 serves as an adjuvant augmenting flagellin-TLR5 mediated NF-kappaB activation even in human
Tano et al., Gan To Kagaku Ryoho 2005 (Carcinoma, Squamous Cell...) : OK-PSA stimulation resulted in the activation of NF-kappaB in the 4 SCC cell lines which express both TLR4 and MD-2 genes, and in 5 SGC cell lines which express at least TLR4 gene independently of MD-2 expression
Chen et al., J Biol Chem 2006 : Overexpression of the NF-kappaB signaling super inhibitor mutant IkappaBalpha ( S32A/S36A ) abolished the effect of p65 and blocked NGF induced activation of NF-kappaB signaling, resulting in a significant reduction in dor promoter activity
Amin et al., Blood 2006 : Antisense oligodeoxynucleotides ( ODNs ) and inhibitors of Src, PI3K, p38, and NFkappaB significantly reduced rhMIF induced MN VCAM-1 and ICAM-1 expression ( P < .05 ) ... Antisense oligodeoxynucleotides ( ODNs ) and inhibitors of Src, PI3K, p38, and NFkappaB significantly reduced rhMIF induced MN VCAM-1 and ICAM-1 expression ( P < .05 )
Athanasopoulos et al., Blood 2006 (Disease Models, Animal...) : In vitro, Eap significantly reduced intercellular adhesion molecule 1 ( ICAM-1 ) -dependent leukocyte-endothelial interactions and diminished the consequent activation of the proinflammatory transcription factor nuclear factor kappaB (NFkappaB) in leukocytes associated with a decrease in expression of tissue factor
Le Clorennec et al., Blood 2006 (Cell Transformation, Viral...) : Epstein-Barr virus ( EBV ) induces CD95 expression and the CD95 gene ( FAS ) is regulated by NF-kappaB , STAT1, and/or p53 ... Therefore, LMP1 activation of NF-kappaB sensitizes infected B cells to CD95 mediated apoptosis and renders EBV latency III immortalized B cells susceptible to elimination by the immune system, contributing to the establishment of a host/virus equilibrium
Wei et al., Mol Pharmacol 2006 : In addition, fluvastatin increased IL-1beta induced p65 nuclear translocation and nuclear factor kappaB (NF-kappaB) activity, although it inhibited those induced by LPS ... We found that Y-27632 potentiated IL-1beta induced iNOS expression, p65 nuclear translocation, IkappaB kinase (IKK), and NF-kappaB activation, whereas it had minimal effects on LPS induced responses ... Further studies showed that LPS down-regulated Rho and ROCK activity, whereas IL-1beta increased them, suggesting a negative role of Rho and ROCK signaling, which is regulated in contrary manners by IL-1beta and LPS, in IKK/NF-kappaB activation ... Further studies showed that LPS down-regulated Rho and ROCK activity, whereas IL-1beta increased them, suggesting a negative role of Rho and ROCK signaling, which is regulated in contrary manners by IL-1beta and LPS, in IKK/NF-kappaB activation
Murakami et al., J Nutr 2005 (Neoplasms) : Pretreatment of RAW264.7 cells with LPS led to the activation of mitogen activated protein kinase ( MAPK ) s [ p38, extracellular signal regulated kinase ( ERK ) 1/2, c-Jun NH2-terminal kinase (JNK)1/2 ] and Akt, together with degradation of the inhibitor of nuclear factor-kappaB ( IkappaB)-alpha protein and nuclear translocation of nuclear factor (NF)-kappaB p65 , and the resultant activation of activator protein (AP)-1, NF-kappaB , and cAMP-responsive element binding protein ( CREB ) transcription factors
Ganster et al., J Interferon Cytokine Res 2005 (Inflammation) : In contrast, IFN-gamma inhibits TNF-alpha induced transcription of an NF-kappaB reporter gene in a Stat1 dependent mechanism in 2fTGH fibroblasts ... In contrast, IFN-gamma inhibits TNF-alpha induced transcription of an NF-kappaB reporter gene in a Stat1 dependent mechanism in 2fTGH fibroblasts ... IFN-gamma and Stat1 dependent inhibition of NF-kappaB transcription occurs independent of TNF-alpha induced NF-kappaB DNA binding ... IFN-gamma and Stat1 dependent inhibition of NF-kappaB transcription occurs independent of TNF-alpha induced NF-kappaB DNA binding ... Further, ChIP analysis was applied to detect cytokine induced in vivo binding and transcriptional regulation of the human inducible nitric oxide synthase (iNOS) gene by NF-kappaB and Stat1
Voss et al., J Biol Chem 2006 (Crohn Disease) : Luciferase gene reporter analyses and site directed mutagenesis experiments demonstrated that functional binding sites for NF-kappaB and AP-1 in the hBD-2 promoter are required for NOD2 mediated induction of hBD-2 through MDP
Smith et al., Glycobiology 2006 : Helicobacter pylori and toll-like receptor agonists induce syndecan-4 expression in an NF-kappaB dependent manner ... The H. pylori- and TLR induced increase in syndecan-4 mRNA was blocked by the proteosome inhibitor MG-132 suggesting a role for nuclear factor kappaB (NF-kappaB) in the regulation of syndecan-4 gene expression ... These results thus demonstrate that the response of the syndecan-4 gene to infectious agents, or their products, is a direct result of NF-kappaB binding to the promoter and induction of de novo transcription
Shin et al., EMBO J 2006 : Notch1 augments NF-kappaB activity by facilitating its nuclear retention ... Here, we examined the temporal relationship between Notch signaling and NF-kappaB induction during T-cell activation
Cheong et al., J Biol Chem 2006 : We undertook an iterative computational and experimental investigation of the dynamic properties of tumor necrosis factor (TNF)alpha mediated activation of the transcription factor NF-kappaB
Corbetta et al., Endocr Relat Cancer 2005 (Parathyroid Neoplasms) : Although TNFalpha was poorly effective in increasing cyclin D1 expression, NF-kappaB blockade by the specific inhibitor BAY11-7082 reduced FCS stimulated cyclin D1 by about 60 %
Su et al., Eur J Immunol 2006 : Overexpression of TRAF1 inhibited TRIF- and TLR3 mediated activation of NF-kappaB , IFN stimulated response element and the IFN-beta promoter
Yip et al., Phytomedicine 2005 : Luciferase reporter gene assays further demonstrated that sKLK inhibited both basal and tumor necrosis factor-alpha stimulated NFkappaB activation
Su et al., Exp Cell Res 2006 (MAP Kinase Signaling System) : Although both DR3 and TNFRI activated TRAF2 and NF-kappaB to induce IL-8 gene transcription, the kinase cascades that transduce signals for DR3- and TNFRI induced IL-8 gene transcription are different
Frémont et al., Life Sci 2006 (Lymphoma, B-Cell) : PKR is also involved in the activation of nuclear factor kappaB (NFkappaB) through activation of the IkappaB kinase complex
Lee et al., Neurosci Lett 2006 (Encephalitis...) : We also found that adenosine as well as Cl-IB-MECA inhibited LPS induced NF-kappaB DNA binding and luciferase reporter activity
Grassl et al., Int J Med Microbiol 2005 : By means of electrophoretic mobility shift assays we demonstrate that sesquiterpene lactones inhibit Yersinia triggered activation of NF-kappaB by inhibiting Rel p65 , but not Rel p50
Chandrasekar et al., Biochem Biophys Res Commun 2006 : Interleukin-18 induces human cardiac endothelial cell death via a novel signaling pathway involving NF-kappaB dependent PTEN activation ... Our results demonstrate that IL-18 reduced phospho-Akt and bcl-2 levels, stimulated NF-kappaB activation, and induced PTEN-promoter-reporter activity, mRNA expression, and protein levels in HCMEC ... IL-18 induced activation of NF-kappaB and PTEN upregulation were mediated by p38MAPK ... IL-18 induced activation of NF-kappaB and PTEN upregulation were mediated by p38MAPK ... IL-18 induced activation of NF-kappaB and PTEN upregulation were mediated by p38MAPK
Yamaguchi et al., FEBS Lett 2005 : Therefore, in the present study, we investigated whether globular adiponectin ( gAd ) would be able to inhibit TLR mediated nuclear factor-kappaB (NF-kappaB) signaling in mouse macrophages ( RAW264 ) ... gAd predominantly bound to the AdipoR1 receptor and suppressed TLR mediated NF-kappaB signaling ... gAd mediated inhibition of TLR mediated IkappaB phosphorylation and NF-kappaB activation was eliminated by the pretreatment of cycloheximide
Albee et al., Inflamm Res 2006 (Inflammation) : E. coli infection induces caspase dependent degradation of NF-kappaB and reduces the inflammatory response in macrophages ... Inhibition of caspase activity resulted in increased DNA binding activity of NF-kappaB and enhanced production of NF-kappaB dependent reporter gene expression following infection ... Further, suppression of caspase activity reduced the proteolytic cleavage of NF-kappaB ... These data indicate that infection of macrophages with E. coli induces a caspase dependent inhibition of NF-kappaB that results in a reduced production of pro-inflammatory cytokines and impaired clearance of bacteria
Jewett et al., Cancer Immunol Immunother 2006 (Laryngeal Neoplasms...) : Moreover, NFkappaB mediated increase in IL-6 secretion by oral tumors may in part be responsible for the observed inactivation and death of the immune effectors
Yuan et al., Mol Biol Rep 2005 : Reporter gene assays showed that overexpression of RHEBL1 in HEK 293T cells strongly activated the transcriptional activities of NF-kappa B , while the mutant ( D60K ) only weakly activates NF-kappa B-mediated transcription
Lee et al., Mol Cell Biochem 2006 : Rac1 GTPase regulates osteoclast differentiation through TRANCE induced NF-kappa B activation
Maksimow et al., J Leukoc Biol 2006 : Fas costimulation of naive CD4 T cells is controlled by NF-kappaB signaling and caspase activity ... Inhibition of NF-kappaB simultaneously with Fas ligation inhibited the increased IFN-gamma production and caused uniform death of all responding T cells
Wu et al., J Hypertens 2006 : Moreover, Ang II increased the phosphorylation of NF-kappaB inducing kinase (NIK) , and this increase was significantly inhibited by nifedipine
Shishodia et al., Oncogene 2006 : Diosgenin suppressed TNF induced NF-kappaB activation as determined by DNA binding, activation of IkappaBalpha kinase, IkappaBalpha phosphorylation, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation through inhibition of Akt activation
Rubio et al., Oncogene 2006 (Breast Neoplasms...) : We have found that TNF-alpha exerts a mitogenic effect, inducing cyclin D1 expression and activation of the transcription factor NF-kappaB ... Importantly, activation of NF-kappaB was required for estrogen induced proliferation and cyclin D1 expression
Manna et al., J Cell Physiol 2006 : In this report we provide evidences that oleandrin, a cardiac glycoside potentially inhibited IL-8-, formyl peptide ( FMLP ) -, EGF-, or nerve growth factor (NGF)-, but not IL-1- or TNF induced NF-kappaB activation in macrophages ... Phospholipids significantly protected oleandrin mediated inhibition of IL-8 binding thereby restoring IL-8 induced NF-kappaB activation
Weber-Mzell et al., Pediatr Surg Int 2006 (Cholestasis) : Likewise, Curcumin and ICK suppressed NF-kappaB activation, but this did not reach significance in this experiment
Fan et al., Brain Res 2006 : The purpose of the study is to determine if expression or secretion of brain derived neurotrophic factor (BDNF) in retinal ganglion cells ( RGC-5 ) is mediated by NFkappaB or Ca2+/calmodulin dependent protein kinase II ( CaMKII )
Weber et al., Bioorg Med Chem 2006 : Enone analogues of curcumin were compared with curcumin for their abilities to inhibit the TNFalpha induced activation of NFkappaB , using the Panomics ' NFkappaB Reporter Stable Cell Line
Huber et al., Placenta 2006 : Induction of the inhibitor may involve TNFalpha stimulated activation of NFkappaB
Jalal et al., J Am Soc Nephrol 2006 : Src activation of NF-kappaB augments IL-1beta induced nitric oxide production in mesangial cells ... In agreement with the pharmacologic inhibition studies, siRNA directed against c-Src specifically limited c-Src protein expression and inhibited IL-1beta mediated induction of NF-kappaB DNA binding activity, whereas control siRNA had no effect ... In agreement with the pharmacologic inhibition studies, siRNA directed against c-Src specifically limited c-Src protein expression and inhibited IL-1beta mediated induction of NF-kappaB DNA binding activity, whereas control siRNA had no effect ... Conversely, overexpression of constitutively active c-Src augmented basal and IL-1beta mediated induction of NF-kappaB DNA binding activity and NO production ... Conversely, overexpression of constitutively active c-Src augmented basal and IL-1beta mediated induction of NF-kappaB DNA binding activity and NO production ... Thus, SFK play a key role in IL-1beta induced NO production in mesangial cells and do so via tyrosine phosphorylation of IkappaBalpha and consequent NF-kappaB activation
Arechiga et al., J Immunol 2005 : Cutting edge : FADD is not required for antigen receptor mediated NF-kappaB activation ... Thus, FADD does not play an essential role in NF-kappaB activation, suggesting an alternative route by which this adaptor promotes the clonal expansion of T cells
Chen et al., Am J Physiol Heart Circ Physiol 2006 : In contrast, Nrf2 did not inhibit TNF-alpha induced NF-kappaB activation
Molestina et al., J Cell Sci 2005 (Toxoplasmosis) : In the present study, we examined the roles of host IKK and parasite derived TgIKK on the temporal modulation of NF-kappaB activation
Krüll et al., Am J Respir Cell Mol Biol 2006 : In addition, the chemical inhibitor as well as dominant negative mutants of p38 MAPK isoforms p38alpha, beta2, and gamma inhibited C. pneumoniae related NF-kappaB activation ... Our data demonstrate that C. pneumoniae triggers an early proinflammatory signaling cascade involving p38 MAPK dependent NF-kappaB activation, resulting in subsequent GM-CSF release
Zhang et al., Pancreas 2006 (Neoplasm Invasiveness...) : Epidermal growth factor promotes invasiveness of pancreatic cancer cells through NF-kappaB mediated proteinase productions ... Furthermore, EGF stimulated NF-kappaB binding activity ... Moreover, pretreatment of cells with NF-kappaB inhibitors, pyrrolidine dithiocarbamate or ibuprofen, markedly attenuated EGF induced NF-kappaB activation ... Subsequently, the EGF induced MMP-9 and uPA expression and MMP-9 activity, as well as cell invasiveness, were also inhibited by these NF-kappaB inhibitors ... Subsequently, the EGF induced MMP-9 and uPA expression and MMP-9 activity, as well as cell invasiveness, were also inhibited by these NF-kappaB inhibitors ... Our findings indicated that NF-kappaB mediated MMP-9 and uPA induction was responsible for EGF induced invasiveness in these pancreatic cancer cell lines and implicate that such anti-NF-kappaB therapy as the use of NF-kappaB inhibitors may contribute to the reduction of invasiveness of pancreatic cancer
Kim et al., Cytokine 2005 : Involvement of mitogen activated protein kinase and NF-kappaB activation in Ca2+ induced IL-8 production in human mast cells ... NF-kappaB inhibitors blocked the ability of Ca ( 2+ ) to induce IL-8 production, and the activation of NF-kappaB was required for intracellular Ca ( 2+ ) -induced up-regulation of IL-8
Ogino et al., Biochim Biophys Acta 2005 : We have previously reported that monochloramine ( NH ( 2 ) Cl ), a neutrophil derived oxidant, inhibited tumor necrosis factor alpha (TNFalpha) induced expression of cell adhesion molecules and nuclear factor-kappaB (NF-kappaB) activation ( Free Radical Research 36 ( 2002 ) 845-852 ) ... Here, we studied the mechanism how NH ( 2 ) Cl inhibited TNFalpha induced NF-kappaB activation, and compared the effects with taurine chloramine ( Tau-NHCl ) ... Although Tau-NHCl alone had little effects on TNFalpha induced NF-kappaB activation, simultaneous presence of Tau-NHCl and ammonium ion significantly inhibited the NF-kappaB activation, probably through the conversion of Tau-NHCl to NH ( 2 ) Cl ... These results indicated that NH ( 2 ) Cl inhibited TNFalpha induced NF-kappaB activation through the oxidation of IkappaB, and that NH ( 2 ) Cl is physiologically more relevant than Tau-NHCl in modifying NF-kappaB mediated cellular responses
Baetz et al., Circulation 2005 (Anoxia) : Collectively, our data provide new mechanistic insight into the mode by which NF-kappaB suppresses cell death and identify BNIP3 as a key transcriptional target for NF-kappaB regulated expression in ventricular myocytes
Szabo et al., Alcohol Clin Exp Res 2005 (Fatty Liver) : Increased liver injury in the MCD diet fed TLR2 mice was associated with reduced baseline and LPS induced NF-kB and PPRE binding compared to MCS controls
Bryce et al., Int Immunol 2006 (Hypersensitivity...) : We have previously reported that tumor necrosis factor receptor associated factor 1 ( TRAF1 ), an intracellular protein, which binds to a range of molecules, including tumor necrosis factor (TNF) receptor family members, regulates TNF induced NF-kappaB and AP-1 signaling as well as TCR triggered proliferative responses in T cells ... We have previously reported that tumor necrosis factor receptor associated factor 1 ( TRAF1 ), an intracellular protein, which binds to a range of molecules, including tumor necrosis factor (TNF) receptor family members, regulates TNF induced NF-kappaB and AP-1 signaling as well as TCR triggered proliferative responses in T cells
Taggart et al., J Exp Med 2005 : We have previously shown that SLPI can inhibit LPS induced NF-kappaB activation in monocytic cells by inhibiting degradation of IkappaBalpha without affecting the LPS induced phosphorylation and ubiquitination of IkappaBalpha ... We have previously shown that SLPI can inhibit LPS induced NF-kappaB activation in monocytic cells by inhibiting degradation of IkappaBalpha without affecting the LPS induced phosphorylation and ubiquitination of IkappaBalpha ... We have previously shown that SLPI can inhibit LPS induced NF-kappaB activation in monocytic cells by inhibiting degradation of IkappaBalpha without affecting the LPS induced phosphorylation and ubiquitination of IkappaBalpha ... Here, we present evidence to show that upon incubation with peripheral blood monocytes ( PBMs ) and the U937 monocytic cell line, SLPI enters the cells, becoming rapidly localized to the cytoplasm and nucleus, and affects NF-kappaB activation by binding directly to NF-kappaB binding sites in a site-specific manner ... Therefore, SLPI inhibition of NF-kappaB activation is mediated, in part, by competitive binding to the NF-kappaB consensus binding site
Li et al., Mol Cell Biol 2006 : Clearance of both superoxide and H2O2 from within the endosomal compartment significantly abrogated IL-1beta dependent IKK and NF-kappaB activation ... Small interfering RNAs to either MyD88 or Rac1 inhibited IL-1beta induction of endosomal superoxide and NF-kappaB activation
Heiss et al., Antioxid Redox Signal 2005 : Time dependent modulation of thioredoxin reductase activity might contribute to sulforaphane mediated inhibition of NF-kappaB binding to DNA
Lee et al., Antioxid Redox Signal 2005 : Curcumin inhibits phorbol ester induced up-regulation of cyclooxygenase-2 and matrix metalloproteinase-9 by blocking ERK1/2 phosphorylation and NF-kappaB transcriptional activity in MCF10A human breast epithelial cells
Watanabe et al., Peptides 2006 : Furthermore, both an angiotensin-converting-enzyme (ACE) inhibitor and an AT1 receptor antagonist enhanced ( rather than reduced ) the NF-kappaB and AP-1 activities in the hippocampus and/or cerebellum of rats given LPS
Sommer et al., Immunity 2005 : PKC isoforms and CARMA1 play crucial roles in immunoreceptor dependent NF-kappaB activation ... Together, our data support a model whereby CARMA1 phosphorylation controls NF-kappaB activation by triggering a shift from an inactive to an active CARMA1 conformer
Matsumoto et al., Immunity 2005 : CARMA1 mediates T cell receptor ( TCR ) -induced NF-kappaB activation
Eddy et al., Cancer Res 2005 (Breast Neoplasms) : Expression of a kinase-inactive IKK-i/IKKepsilon mutant in breast cancer cells reduced NF-kappaB activity as judged by transfection assays of reporters driven either by NF-kappaB elements or the promoters of two NF-kappaB target genes, cyclin D1 and relB
Wang et al., Am J Respir Cell Mol Biol 2006 (Inflammation) : LPS induced activation of NF-kappaB and AP-1 determined by electrophoretic mobility shift assay were significantly reduced by overexpressing caveolin-1 in RAW264.7 cells ... LPS induced activation of NF-kappaB and AP-1 determined by electrophoretic mobility shift assay were significantly reduced by overexpressing caveolin-1 in RAW264.7 cells
Janssens et al., Cell 2005 : PIDD mediates NF-kappaB activation in response to DNA damage ... We now demonstrate that PIDD plays a critical role in DNA-damage induced NF-kappaB activation ... PIDD expression enhances genotoxic-stress induced NF-kappaB activation through augmented sumoylation and ubiquitination of NEMO
Liang et al., Biochem Pharmacol 2006 : Epoxyquinone A monomer ( EqM ), a synthetic derivative of the natural product epoxyquinol A, has previously been shown to be a potent inhibitor of tumor necrosis factor-alpha (TNF-alpha) induced activation of NF-kappaB , but the mechanism by which EqM inhibits NF-kappaB activation was not known
Shin et al., Biochim Biophys Acta 2005 : Our findings indicate that transcription of the CCL15 gene is regulated by AP-1 and NF-kappaB through MEK and JNK MAPK pathways in monocytoid cells
Kim et al., J Immunol 2006 : In addition, the p43 stimulated IL-12 induction and NF-kappaB DNA binding activity were significantly suppressed by caffeic acid phenethyl ester and BAY11-7082, both inhibitors of NF-kappaB activation, indicating that p43 induced the production of IL-12 in macrophages mainly via the activation of NF-kappaB
Sekine et al., J Immunol 2006 : Disruption of STAP-2 resulted in impaired LPS/TLR4 induced cytokine production and NF-kappaB activation ... Disruption of STAP-2 resulted in impaired LPS/TLR4 induced cytokine production and NF-kappaB activation ... Disruption of STAP-2 resulted in impaired LPS/TLR4 induced cytokine production and NF-kappaB activation
Granja et al., J Immunol 2006 : Furthermore, we present evidence that A238L inhibits the activation of TNF-alpha by modulating NF-kappaB , NF-AT, and c-Jun trans activation through a mechanism that involves CREB binding protein/p300 function, because overexpression of these transcriptional coactivators recovers TNF-alpha promoter activity
Opitz et al., J Immunol 2006 : RNA interference and Nod1-overexpression experiments demonstrated that Nod1 is critically involved in chemokine secretion and NF-kappaB activation initiated by L. monocytogenes in human endothelial cells ... In conclusion, L. monocytogenes induced a Nod1 dependent activation of p38 MAPK signaling and NF-kappaB which resulted in IL-8 production in endothelial cells
Lee et al., J Immunol 2006 : Our results suggest that IL-1beta stimulated transcription of TGF-beta1 is temporally regulated by NF-kappaB and AP-1 and involves histone hyperacetylation at distinct promoter sites
Bhatnagar et al., Cell Microbiol 2006 (Mycobacterium avium-intracellulare Infection) : Interestingly, TNF-alpha secretion by macrophages infected with SmO or SmT 2151 was dependent on p38, ERK1/2 and NF-kappaB while TNF-alpha secretion by Rg 2151 infected macrophages was dependent on NF-kappaB but not the MAPKs
Qiu et al., Shock 2006 (Liver Failure...) : These results suggest that FN protected against GalN/LPS induced liver failure by a mechanism involving inhibition of NF-kappaB activation, which caused down-regulation of TNF-alpha and involved up-regulation of IL-10 , and elevation of Bcl-xL induced a blockage of apoptotic signals, by which apoptosis of hepatocytes caused by GalN/LPS was suppressed
Li et al., Biomed Environ Sci 2005 : Alpha-zearalenol inhibits angiotensin II-induced integrin beta3 mRNA expression by suppressing NF-kappaB activation in endothelial cells
Karuri et al., J Pharmacol Exp Ther 2006 (Endotoxemia...) : Inasmuch as iNOS expression can be regulated by nuclear factor (NF) kappaB , mechanistic studies show that D3T blocks NFkappaB nuclear translocation and DNA binding and that these effects are accompanied by changes in the levels of phospho-inhibitor of NFkappaB
Karadag et al., Blood 2006 (MAP Kinase Signaling System...) : Furthermore, inhibitors of p38 MAPK and cPLA2, but not NF-kappaB and JAK2, signaling pathways inhibited ADAM-9 induced IL-6 production by hOBs ( P < .01 )
Chong et al., Curr Neurovasc Res 2005 (Alzheimer Disease...) : Erythropoietin requires NF-kappaB and its nuclear translocation to prevent early and late apoptotic neuronal injury during beta-amyloid toxicity
Jang et al., Life Sci 2006 : In this study, the effects of scoparone on the expression of interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) and activation of nuclear factor-kappaB (NF-kappaB) were examined in U937 human monocytes activated with phorbol 12-myristate 13-acetate ( PMA ) ... In this study, the effects of scoparone on the expression of interleukin-8 (IL-8) and monocyte chemotactic protein-1 (MCP-1) and activation of nuclear factor-kappaB (NF-kappaB) were examined in U937 human monocytes activated with phorbol 12-myristate 13-acetate ( PMA )
Takada et al., J Biol Chem 2006 (Neoplasm Invasiveness) : We found that in several human cancer cell lines, SAHA potentiated the apoptosis induced by tumor necrosis factor (TNF) and chemotherapeutic agents and inhibited TNF induced invasion and receptor activator of NF-kappaB ligand induced osteoclastogenesis, all of which are known to require NF-kappaB activation ... Because several of these genes are regulated by NF-kappaB, we postulated that SAHA mediates its effects by modulating NF-kappaB and found that SAHA suppressed NF-kappaB activation induced by TNF , IL-1beta, okadaic acid, doxorubicin, lipopolysaccharide, H ( 2 ) O ( 2 ), phorbol myristate acetate, and cigarette smoke ; the suppression was not cell type-specific because both inducible and constitutive NF-kappaB activation was inhibited ... Because several of these genes are regulated by NF-kappaB, we postulated that SAHA mediates its effects by modulating NF-kappaB and found that SAHA suppressed NF-kappaB activation induced by TNF, IL-1beta , okadaic acid, doxorubicin, lipopolysaccharide, H ( 2 ) O ( 2 ), phorbol myristate acetate, and cigarette smoke ; the suppression was not cell type-specific because both inducible and constitutive NF-kappaB activation was inhibited
Wang et al., Nat Immunol 2006 : Tumor necrosis factor receptor associated factor 6 ( TRAF6 ) is critical for mediating Toll-like receptor ( TLR ) -interleukin 1 receptor (IL-1R) signaling and subsequent activation of NF-kappaB and AP-1, transcriptional activators of innate immunity ... Tumor necrosis factor receptor associated factor 6 ( TRAF6 ) is critical for mediating Toll-like receptor ( TLR ) -interleukin 1 receptor (IL-1R) signaling and subsequent activation of NF-kappaB and AP-1, transcriptional activators of innate immunity ... Tumor necrosis factor receptor associated factor 6 ( TRAF6 ) is critical for mediating Toll-like receptor ( TLR ) -interleukin 1 receptor (IL-1R) signaling and subsequent activation of NF-kappaB and AP-1, transcriptional activators of innate immunity ... Formation of the beta-arrestin-TRAF6 complex prevented autoubiquitination of TRAF6 and activation of NF-kappaB and AP-1
Nichols et al., J Virol 2006 : We report here that expression of MC160 molecules did significantly reduce TNF-alpha mediated NF-kappaB activation in 293T cells, as measured by gene reporter and gel mobility shift assays
Vinolo et al., J Biol Chem 2006 (Ectodermal Dysplasia...) : A point mutation in NEMO associated with anhidrotic ectodermal dysplasia with immunodeficiency pathology results in destabilization of the oligomer and reduces lipopolysaccharide- and tumor necrosis factor mediated NF-kappa B activation ... In addition, functional complementation assays using NEMO-deficient pre-B and T lymphocytes showed that the pathogenic mutation reduced TNF-alpha and LPS induced NF-kappaB activation by altering the assembly of the IKK complex
Kim et al., FEBS Lett 2006 : In summary, hirsutenone inhibits the TPA induced upregulation of COX-2 and MMP-9 in human breast epithelial cells, possibly by targeting NF-kappaB , which may contribute to its chemopreventive effects ... In summary, hirsutenone inhibits the TPA induced upregulation of COX-2 and MMP-9 in human breast epithelial cells, possibly by targeting NF-kappaB , which may contribute to its chemopreventive effects
Cheng et al., American journal of physiology. Renal physiology 2006 : Electrophoretic mobility shift assay indicated that SNAP stimulated nuclear factor (NF)-kappaB binding activity in cTAL that was also inhibited by the p38 inhibitor
Ito et al., J Exp Med 2006 (Pulmonary Disease, Chronic Obstructive) : Loss of HDAC2 did not reduce GR nuclear translocation, GR binding to glucocorticoid response element ( GRE ) on DNA, or GR-induced DNA or gene induction but inhibited the association between GR and NF-kappaB
Killeen et al., J Pharmacol Exp Ther 2006 : Treating these cells with pyrrolidine dithiocarbamate, SN50 ( amino acid sequence AAVALLPAVLLALLAPVQRKRQKLMP ) or 5- ( thien-3-yl ) -3-aminothiophene-2-carboxamide ( SC-514 ) also inhibited LPS induced NF-kappaB DNA binding, but these compounds failed to inhibit LPS induced HMGB1 secretion
Sadowska et al., Pharmacol Res 2006 : Neutrophils from healthy volunteers ( NAC naïve ) were pre incubated with NAC for 30 min and the effects on the release of elastase and IL-8, the respiratory burst in response to fMLP and PMA, on TNFalpha induced NFkappaB activation and on the migration across an endothelial-epithelial bilayer were investigated ... Neutrophils from healthy volunteers ( NAC naïve ) were pre incubated with NAC for 30 min and the effects on the release of elastase and IL-8 , the respiratory burst in response to fMLP and PMA, on TNFalpha induced NFkappaB activation and on the migration across an endothelial-epithelial bilayer were investigated
Isoda et al., Arterioscler Thromb Vasc Biol 2006 (Atherosclerosis...) : Investigation of potential signaling pathways demonstrated that metformin diminished IL-1beta induced activation and nuclear translocation of nuclear factor-kappa B (NF-kappaB) in SMCs
Mullan et al., Arthritis Rheum 2006 (Arthritis, Rheumatoid...) : A-SAA induced expression of VCAM-1 , ICAM-1, and MMP-1 was down-regulated by NF-kappaB inhibition ... A-SAA induced expression of VCAM-1, ICAM-1 , and MMP-1 was down-regulated by NF-kappaB inhibition ... A-SAA induced expression of VCAM-1, ICAM-1 , and MMP-1 was down-regulated by NF-kappaB inhibition ... A-SAA induced expression of VCAM-1, ICAM-1, and MMP-1 was down-regulated by NF-kappaB inhibition ... Furthermore, A-SAA induced IkappaBalpha degradation and NF-kappaB translocation, suggesting that its proinflammatory effects are mediated in part by NF-kappaB signaling
López-Pedrera et al., Arthritis Rheum 2006 (Antiphospholipid Syndrome) : At the molecular level, increased proteolysis of IkappaBalpha and activation of NF-kappaB were observed
Collino et al., Eur J Pharmacol 2006 (Brain Ischemia...) : Pre-treatment with either rosiglitazone or pioglitazone significantly reduced oxidative stress, COX-2 protein expression and activation of MAPKs and NF-kappaB ... Pre-treatment with either rosiglitazone or pioglitazone significantly reduced oxidative stress, COX-2 protein expression and activation of MAPKs and NF-kappaB
Jones et al., J Infect Dis 2006 (Escherichia coli Infections...) : Neutrophil recruitment requires nuclear factor (NF)- kappa B, but IL-6 was neither sufficient nor essential to induce NF- kappa B-mediated gene expression in the lungs
Fritz et al., J Biomed Mater Res A 2006 (Prosthesis Failure) : Inhibition studies with N-acetyl-L-cysteine (Nac) and MG-132 suggest that titanium particle activation of NF-kappaB activity and IL-8 chemokine expression involves oxidant signaling and IkappaBalpha-proteasomal degradation
Vuolteenaho et al., Scand J Rheumatol 2005 (Osteoarthritis, Knee) : This can be explained by the fact that the four effective DMARDs also suppressed IL-1beta induced activation of nuclear factor kappa B (NF-kappaB) , which is a crucial transcription factor for iNOS
Maezawa et al., J Immunol 2006 (MAP Kinase Signaling System) : We also found that IL-25R mediated NF-kappaB activation was inhibited by the expression of dominant negative TRAF6 but not of dominant negative TRAF2 ... We also found that IL-25R mediated NF-kappaB activation was inhibited by the expression of dominant negative TRAF6 but not of dominant negative TRAF2 ... We also found that IL-25R mediated NF-kappaB activation was inhibited by the expression of dominant negative TRAF6 but not of dominant negative TRAF2 ... Furthermore, IL-25R mediated NF-kappaB activation , but not MAPK activation, was diminished in TRAF6-deficient murine embryonic fibroblast ... Taken together, these results indicate that TRAF6 plays a critical role in IL-25R mediated NF-kappaB activation and gene expression ... Taken together, these results indicate that TRAF6 plays a critical role in IL-25R mediated NF-kappaB activation and gene expression
Zha et al., J Immunol 2006 : RFP inhibited activation of the IFN stimulated response element and/or NF-kappaB mediated by the IKK family members and triggered by TNF, IL-1, polyinosinic-polycytidylic acid ( ligand for TLR3 ), and viral infection
Márquez et al., Mol Pharmacol 2006 : Arvanil also prevents tumor necrosis factor-alpha induced nuclear factor-kappaB (NF-kappaB) activation by direct inhibition of IkappaBalpha degradation, NF-kappaB binding to DNA, and NF-kappaB dependent transcription
Hu et al., J Clin Invest 2006 (Lymphoma, B-Cell, Marginal Zone...) : While both BCL10 and MALT1 are critically involved in antigen receptor mediated NF-kappaB activation, the role of cIAP2 is not clear ... While both BCL10 and MALT1 are critically involved in antigen receptor mediated NF-kappaB activation, the role of cIAP2 is not clear ... Furthermore, BCL10 and cIAP2-MALT1 synergistically activate NF-kappaB ... Furthermore, BCL10 and cIAP2-MALT1 synergistically activate NF-kappaB ... Furthermore, BCL10 and cIAP2-MALT1 synergistically activate NF-kappaB ... Furthermore, BCL10 and cIAP2-MALT1 synergistically activate NF-kappaB
Nenci et al., Hum Mol Genet 2006 (Disease Models, Animal...) : NF-kappaB essential modulator (NEMO) , the regulatory subunit of the IkappaB kinase, is essential for NF-kappaB activation
Amcheslavsky et al., J Cell Physiol 2006 : Since both RANKL-RANK and CpG-ODN-TLR9 interactions result in NF-kappaB activation, p38 and ERK phosphorylation, and TNF-alpha synthesis ( all implicated in osteoclastogenesis ), we hypothesized that CpG-ODN ( but not RANKL ) in addition induces the synthesis of an anti-osteoclastogenic factor ... Since both RANKL-RANK and CpG-ODN-TLR9 interactions result in NF-kappaB activation, p38 and ERK phosphorylation, and TNF-alpha synthesis ( all implicated in osteoclastogenesis ), we hypothesized that CpG-ODN ( but not RANKL ) in addition induces the synthesis of an anti-osteoclastogenic factor
Hwang et al., Mol Carcinog 2006 (Neoplasm Invasiveness...) : The LPA induced NF-kappaB DNA binding was preceded by transient IkappaBalpha phosphorylation, and decreased total IkappaBalpha levels ... The LPA induced Matrigel invasion and NF-kappaB DNA binding activity were both inhibited by expression of the RhoA inhibitor C3 exoenzyme or dominant negative mutant NF-kappaB inhibitor IkappaBalpha S32/36A ... Each inhibitor blocked LPA induced invasion while PDTC inhibited LPA induced NF-kappaB and invasion to the greatest extent
Sotiriou et al., FASEB J 2006 (Atherosclerosis...) : Through its interaction with Mac-1, Lp(a) induced activation of the proinflammatory transcription factor NFkappaB , as well as the NFkappaB related expression of prothrombotic tissue factor
Kim et al., Mol Cells 2005 : Nuclear factor-kB (NF-kB) is a critical transcription factor in RANKL induced osteoclastogenesis, and electrophoretic mobility shift assays ( EMSAs ) and assay of NF-kB mediated secreted alkaline phosphatase ( SEAP ) revealed that sulforaphane selectively inhibited NF-kappaB activation induced by RANKL
Wang et al., Plastic and reconstructive surgery 2006 : Transfer of exogenous PDGF gene to ischemic flaps promotes expression of VEGF and bFGF genes and activation of NF-kappaB gene in addition to its effects on the PDGF gene
Um et al., J Biol Chem 2006 (Neuroblastoma) : In these cells Prl treatment strongly activates the STAT5, AKT, and MAPK signaling pathways and induces weak activation of the p65 NF-kappaB factor
Anrather et al., J Biol Chem 2006 : NF-kappaB regulates phagocytic NADPH oxidase by inducing the expression of gp91phox ... NF-kappaB regulates phagocytic NADPH oxidase by inducing the expression of gp91phox ... We also found that gp91(phox) transcription is dependent on NF-kappaB and we identified two potential cis acting elements in the murine gp91(phox) promoter that control NF-kappaB dependent regulation
Fritz et al., J Biol Chem 2006 : MEKK3 dependent activation of an NF-kappaB reporter gene as well as ERK, JNK, and p38 MAP kinases correlated with a requirement for serine at position 526
Kim et al., Mol Pharmacol 2006 (Colitis) : TauCl inhibited tumor necrosis factor (TNF) dependent NF-kappaB activation by modifying thiol ( s ) on p65 and blocking DNA binding ... Furthermore, combined TauCl/5-ASA treatment additively inhibited TNF dependent NF-kappaB activation ... Together, our data suggest that the colon-specific carrier taurine contributes to the clinical effect of the prodrug by potentiating the inhibitory effect of the active ingredient 5-ASA on a major proinflammatory signal, TNF dependent NF-kappaB activation in the inflamed large intestine
Konishi et al., Oncogene 2006 (Colorectal Neoplasms) : Futhermore, hRFI overexpression resulted in the activation of nuclear factor-kappaB (NF-kappaB)
Kanneganti et al., Nature 2006 (Inflammation) : In contrast, secretion of tumour-necrosis factor-alpha and IL-6 , as well as activation of NF-kappaB and mitogen activated protein kinases ( MAPKs ) were unaffected by cryopyrin deficiency
Wu et al., Kidney Int 2006 : CTGF enhanced the mRNA expression and protein release of fractalkine, MCP-1, and RANTES, the expression of phospho ( P ) -p42/44 mitogen activated protein kinase ( MAPK ), P-phosphoinositide 3-kinase (PI3-K), P-Akt, and activity of nuclear factor-kappaB (NF-kappaB) in mesangial cells ... P-p42/44 MAPK blockade inhibited the CTGF induced expression of P-p42/44 MAPK but not NF-kappaB , and partially decreased the levels of the above chemokines in supernatants ... P-p42/44 MAPK blockade inhibited the CTGF induced expression of P-p42/44 MAPK but not NF-kappaB , and partially decreased the levels of the above chemokines in supernatants ... P-PI3-K blockade downregulated the CTGF stimulated expression of P-PI3-K, P-Akt, and NF-kappaB but not P-p42/44 MAPK, and partially decreased the release of the above chemokines ... LXA ( 4 ) dose-dependently inhibited the CTGF stimulated mRNA expression and protein release of the above chemokines, and the expression of P-p42/44MAPK, P-PI3-K, P-Akt, and NF-kappaB
Grosjean et al., Biochem Biophys Res Commun 2006 (Neovascularization, Pathologic) : This study aimed to address the hypothesis that VEGF mediated survival effects on endothelium involve NFkappaB
N'Guessan et al., Am J Physiol Lung Cell Mol Physiol 2006 (Pneumococcal Infections) : Streptococcus pneumoniae induced p38 MAPK- and NF-kappaB dependent COX-2 expression in human lung epithelium
Reed et al., Dig Dis Sci 2005 (Colitis...) : Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha, IL-1beta, VCAM-1, ICAM-1, E-selectin, CINC-1, MIP-1alpha , and iNOS ... Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha, IL-1beta, VCAM-1, ICAM-1, E-selectin , CINC-1, MIP-1alpha, and iNOS ... Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R , SP, TNFalpha, IL-1beta, VCAM-1, ICAM-1, E-selectin, CINC-1, MIP-1alpha, and iNOS ... Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha , IL-1beta, VCAM-1, ICAM-1, E-selectin, CINC-1, MIP-1alpha, and iNOS ... Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha, IL-1beta, VCAM-1 , ICAM-1, E-selectin, CINC-1, MIP-1alpha, and iNOS ... Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha, IL-1beta, VCAM-1, ICAM-1 , E-selectin, CINC-1, MIP-1alpha, and iNOS ... Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha, IL-1beta , VCAM-1, ICAM-1, E-selectin, CINC-1, MIP-1alpha, and iNOS ... Colon tissue was collected for assessment of histological changes, NF-kappa B activation , myeloperoxidase (MPO) activity, and expression of NK-1R, SP, TNFalpha, IL-1beta, VCAM-1, ICAM-1, E-selectin, CINC-1, MIP-1alpha, and iNOS
Wehkamp et al., J Invest Dermatol 2006 : Pseudomonas aeruginosa- and IL-1beta mediated induction of human beta-defensin-2 in keratinocytes is controlled by NF-kappaB and AP-1 ... Pseudomonas aeruginosa- and IL-1beta mediated induction of human beta-defensin-2 in keratinocytes is controlled by NF-kappaB and AP-1 ... Transcription factor ELISAs indicated that the NF-kappaB heterodimer p50-p65 binds to all three NF-kappaB sites in the hBD-2 promoter upon stimulation of primary keratinocytes with IL-1beta and PA
Lendemans et al., J Endotoxin Res 2006 : In contrast, the broad-spectrum tyrosine kinase inhibitor genistein and the MEK-1 inhibitor ( PD98059 ) abrogated GM-CSF priming of TNF-alpha release and activation of both NF-kappaB and AP-1 ... In contrast, the broad-spectrum tyrosine kinase inhibitor genistein and the MEK-1 inhibitor ( PD98059 ) abrogated GM-CSF priming of TNF-alpha release and activation of both NF-kappaB and AP-1 ... It is concluded that a tyrosine kinase of the GM-CSF triggered ERK1/2 pathway augments the LPS induced NF-kappaB and AP-1 activation
Yoshida et al., Respirology 2006 (Pulmonary Emphysema) : A specific inhibitor of NF-kappaB reduced MMP production by alveolar macrophages from SP-D-/- mice
Wang et al., Cancer Res 2006 (Lung Neoplasms) : Treatment with 17AAG caused degradation of RIP and IKKbeta that, in turn, blocked TNF induced NF-kappaB activation and antiapoptotic gene expression ... These results suggest that the cytotoxicity seen with 17AAG and TNF treatment results from blocking TNF induced NF-kappaB activation
Deering et al., Clinical and vaccine immunology : CVI 2006 : Tumor necrosis factor (TNF) production in PBMC supernatants was measured by an enzyme linked immunosorbent assay after TLR ligand stimulation and was dependent on gene transcription and NF-kappaB activation
Matsumoto et al., FEBS Lett 2006 (Hepatitis) : Expression of APOBEC2 is transcriptionally regulated by NF-kappaB in human hepatocytes ... Inhibition of NF-kappaB activation invariably blocks TNF-alpha induced APOBEC2 expression
Fritzenwanger et al., Cytokine 2006 : STAT3 phosphorylation, the activation of JAK2 and NF-kappaB are involved in this pathway
Bae et al., Int Immunopharmacol 2006 (MAP Kinase Signaling System...) : Furthermore, electrophoretic mobility shift assay analyses revealed that the MSE treatment induced the activation of the NF-kappaB transcription factor
Didierlaurent et al., Mol Cell Biol 2006 : Activation of interleukin-1 (IL-1) receptor ( IL-1R ), Toll-like receptor 2 (TLR2) , and TLR4 triggers NF-kappaB and mitogen activated protein kinase ( MAPK ) -dependent signaling, thereby initiating immune responses ... Activation of interleukin-1 (IL-1) receptor ( IL-1R ), Toll-like receptor 2 (TLR2), and TLR4 triggers NF-kappaB and mitogen activated protein kinase ( MAPK ) -dependent signaling, thereby initiating immune responses ... Activation of interleukin-1 (IL-1) receptor ( IL-1R ), Toll-like receptor 2 (TLR2), and TLR4 triggers NF-kappaB and mitogen activated protein kinase ( MAPK ) -dependent signaling, thereby initiating immune responses ... Activation of interleukin-1 (IL-1) receptor ( IL-1R ), Toll-like receptor 2 (TLR2), and TLR4 triggers NF-kappaB and mitogen activated protein kinase ( MAPK ) -dependent signaling, thereby initiating immune responses
Tao et al., Mol Cell Biol 2006 : Up-regulation of proapoptotic protein Bcl-XS and neuronal apoptosis induced by serum deprivation are blocked by inhibition and/or down-regulation of NF-kappaB and by prevention of K+ loss
Ichikawa et al., Clin Cancer Res 2006 (Bone Resorption...) : We found that treatment of monocytes with guggulsterone suppressed RANKL activated NF-kappaB activation ( as indicated by gel-shift assay ) and that this suppression correlated with inhibition of IkappaBalpha kinase and phosphorylation and degradation of IkappaBalpha , an inhibitor of NF-kappaB ... We found that treatment of monocytes with guggulsterone suppressed RANKL activated NF-kappaB activation ( as indicated by gel-shift assay ) and that this suppression correlated with inhibition of IkappaBalpha kinase and phosphorylation and degradation of IkappaBalpha, an inhibitor of NF-kappaB
Liang et al., J Mol Cell Cardiol 2006 : In conclusion, CRP induced IkappaB degradation, NF-kappaB nuclear translocation and VCAM-1 protein expression in HUVECs and HAECs
Kang et al., Toxicological sciences : an official journal of the Society of Toxicology 2006 : Src tyrosine kinases mediate crystalline silica induced NF-kappaB activation through tyrosine phosphorylation of IkappaB-alpha and p65 NF-kappaB in RAW 264.7 macrophages ... In the present study, we investigated whether Src family TKs play a role in crystalline silica induced NF-kappaB activation and whether NF-kappaB activation requires Src TK-dependent MAPK activity in RAW 264.7 cells, a mouse peritoneal macrophage cell line
Ma et al., Mol Cancer Ther 2006 (Prostatic Neoplasms) : The loss of IKK affected IkappaBalpha/nuclear factor-kappaB (NF-kappaB) interaction and reduced nuclear transport of NF-kappaB, resulting in reduced NF-kappaB activity
Katerinaki et al., Melanoma Res 2006 (Melanoma...) : Sodium salicylate inhibits TNF-alpha induced NF-kappaB activation , cell migration, invasion and ICAM-1 expression in human melanoma cells ... The aim of this study was to investigate the effect of the non-steroidal anti-inflammatory agent sodium salicylate on TNF-alpha induced activation of the transcription factor nuclear factor-kappaB (NF-kappaB) and upregulation of intercellular adhesion molecule-1 ( ICAM-1 ), and TNF-alpha stimulated cell migration and invasion through fibronectin ... Sodium salicylate inhibited TNF-alpha stimulated NF-kappaB activation in melanoma cells in a concentration dependent manner, and this was achieved with pre-incubation times as short as 15 min. TNF-alpha stimulated ICAM-1 expression in HBL cells was also downregulated by sodium salicylate, although in a manner inversely related to the concentration of this agent ... In conclusion, sodium salicylate effectively inhibited TNF-alpha induced upregulation of NF-kappaB , ICAM-1 expression, in-vitro migration and invasion in human melanoma cells, indicating that non-steroidal anti-inflammatory drugs may be a useful therapeutic approach to oppose inflammation induced melanoma invasion and metastasis in vivo
Salonen et al., Br J Pharmacol 2006 : PKC inhibitors did not alter LPS induced activation of NF-kappaB as measured by electrophoretic mobility shift assay
Mandrekar et al., Alcohol Clin Exp Res 2006 (Atherosclerosis) : Acute alcohol consumption inhibited LPS induced DNA binding of the p65/p50 NF-kappaB in monocytes that regulates the expression of both the TNF-alpha and the IL-1beta genes ... Consistent with this, acute alcohol treatment ( 25 mM ) significantly reduced LPS induced activation of an NF-kappaB-driven reporter gene suggesting inhibition of this proinflammatory signaling pathway
Fiset et al., Clin Exp Allergy 2006 : NF-kappaB activation by LPS was inhibited by IL-4 pre-incubation but not when IL-4 was added at the same time as LPS
Paez et al., J Neurosci Res 2006 : In the N19 cell line, aTf stimulated NF-I and NFkappaB activation, but, aside from aTf, only db-cAMP induced mbp transcription
Chae et al., Immunopharmacol Immunotoxicol 2005 : Both the NF-kappaB inhibitors in the presence of SB203580 had more inhibitory effect on IL-6 release ... However, a specific inhibitor of p38 MAPK , SB203580, had no effect on the NF-kappaB activation, and both the NF-kappaB inhibitors failed to reduce the p38 MAPK activation in the IL-1beta stimulated HGFs
Okamoto et al., Genes Cells 2006 : Taken together, Tax inhibits Fas mediated apoptosis by up-regulating c-FLIP expression in HTLV-I infected cells, and NF-kappaB activity plays an essential role in the up-regulation of c-FLIP
Wong et al., Allergy 2006 : Using various selective inhibitors for signaling molecules, we found that the inductions of IL-8, MCP-1, and I-309 were mediated by either SCF activated ERK or TNF-alpha activated p38 MAPK, while the induction of IP-10 by TNF-alpha was mediated by both activated p38 MAPK and NF-kappaB ... The induction of RANTES by SCF or TNF-alpha was mediated by ERK and NF-kappaB , respectively, and SCF induced MIP-1beta release was mediated by ERK ... The induction of RANTES by SCF or TNF-alpha was mediated by ERK and NF-kappaB , respectively, and SCF induced MIP-1beta release was mediated by ERK
He et al., J Biol Chem 2006 : TRAF6 regulates cell fate decisions by inducing caspase 8-dependent apoptosis and the activation of NF-kappaB ... TRAF6 regulates cell fate decisions by inducing caspase 8-dependent apoptosis and the activation of NF-kappaB
Peloponese et al., J Biol Chem 2006 (Cell Transformation, Viral) : Most of the existing data point to Tax activation of NF-kappaB as important for cellular proliferation and transformation
Wilson et al., J Virol 2006 : Furthermore, both ectopically expressed SV5 SH and MuV SH blocked activation of NF-kappaB by TNF-alpha in a reporter gene assay, suggesting that both SH proteins can inhibit TNF-alpha signaling
Dieperink et al., Pediatr Res 2006 (Hyperoxia) : Inhibition of NF-kappaB prevented the hyperoxia induced activation of caspase 3
Aeberli et al., FEBS Lett 2006 (MAP Kinase Signaling System) : This is associated with increased expression of mitogen activated protein kinase ( MAPK ) phosphatase-1 ( MKP-1 ), concomitant decreased phosphorylation of p38 MAPK , but no effect of MIF on nuclear factor kappaB (NF-kappaB) ... This is associated with increased expression of mitogen activated protein kinase ( MAPK ) phosphatase-1 ( MKP-1 ), concomitant decreased phosphorylation of p38 MAPK, but no effect of MIF on nuclear factor kappaB (NF-kappaB)
Muñoz-Espada et al., J Nutr Biochem 2006 (Prostatic Neoplasms) : Therefore, the objective of the investigation was to determine the effects of cyanidin and kaempferol on prostaglandin E2 ( PGE2 ) and COX-2 protein levels, and if peroxisome proliferator activated receptor gamma ( PPARgamma ) and nuclear factor kappaB (NFkappaB) are involved in the expression of COX-2 in prostate cancer cells
Uemura et al., J Biol Chem 2006 : TAK1 is a component of the Epstein-Barr virus LMP1 complex and is essential for activation of JNK but not of NF-kappaB ... Epstein-Barr virus latent membrane protein 1 (LMP1) activates NF-kappaB and c-Jun N-terminal kinase (JNK), which is essential for LMP1 oncogenic activity ... Genetic analysis has revealed that tumor necrosis factor receptor associated factor 6 ( TRAF6 ) is an indispensable intermediate of LMP1 signaling leading to activation of both NF-kappaB and JNK ... Furthermore, we found that ablation of TAK1 resulted in the loss of LMP1 induced activation of JNK but not of NF-kappaB
Waters et al., Arthritis Rheum 2006 (Scleroderma, Diffuse) : IL-1beta signaling required NF-kappaB and MEK activation, along with activation of PKCdelta
Jin et al., Planta Med 2006 : Among these compounds, inulanolides B and D and eupatolide, exhibited potent inhibitory activity on the LPS induced NF-kappaB activation with IC50 values of 0.49 microM, 0.48 microM, and 1.54 microM, respectively
Heinemann et al., J Biol Chem 2006 (Cell Transformation, Viral) : NFkappaB induction by Tio is independent of Src-kinase interaction and tyrosine phosphorylation of Tio
Shibata et al., J Am Soc Nephrol 2006 (Disease Models, Animal...) : Fluvastatin also mitigated tubulointerstitial damage in PAN nephrosis, with the repression of PAN induced NF-kappaB and activator protein-1 activation in the kidneys ... Fluvastatin also mitigated tubulointerstitial damage in PAN nephrosis, with the repression of PAN induced NF-kappaB and activator protein-1 activation in the kidneys
Pontrelli et al., J Am Soc Nephrol 2006 : Lyn inhibition did not have any effect on CD40L induced NF-kappaB activation and MCP-1 expression but abolished PAI-1 induction ... On the contrary, NF-kappaB inhibition significantly reduced only MCP-1 expression
Sanda et al., Leukemia 2006 (Leukemia-Lymphoma, Adult T-Cell) : In this study, we demonstrate that NF-kappaB is constitutively activated in various HTLV-1 infected T-cell lines and ATL derived cell lines irrespectively of Tax expression as evidenced by the phosphorylation of IkappaBalpha and p65 subunit of NF-kappaB, activation of NF-kappaB DNA binding, and upregulation of various target genes including bcl-xL, bcl-2, XIAP, c-IAP1, survivin, cyclinD1, ICAM-1 and VCAM-1
Griffin et al., J Biol Chem 2006 (Astrocytoma...) : Persistent interleukin-1beta signaling causes long term activation of NFkappaB in a promoter-specific manner in human glial cells ... Persistent activation of the transcription factor is seen in numerous chronic inflammatory disease states, and we have previously demonstrated sustained activation of NFkappaB in human glial cells upon stimulation with interleukin (IL)-1beta ... Persistent activation of the transcription factor is seen in numerous chronic inflammatory disease states, and we have previously demonstrated sustained activation of NFkappaB in human glial cells upon stimulation with interleukin (IL)-1beta ... Such sustained activation of NFkappaB is dependent on the continuous presence and activity of IL-1beta ... These studies thus demonstrated that persistent IL-1 signaling causes sustained activation of NFkappaB in a promoter-specific manner in human glial cells, leading to prolonged induction of selective pro-inflammatory genes
Cohen-Lahav et al., Nephrol Dial Transplant 2006 : The increase in IkappaBalpha levels reduces nuclear translocation of NFkappaB and thereby downgrades its activity
Mookherjee et al., J Immunol 2006 (Inflammation) : LL-37 significantly inhibited the expression of specific proinflammatory genes up-regulated by NF-kappaB in the presence of LPS , including NFkappaB1 (p105/p50) and TNF-alpha induced protein 2 (TNFAIP2)
Musette et al., Med Sci (Paris) 2006 (Infection...) : The TLR activation induce the NF-kappaB translocation to the nucleus and cytokine secretion
Kim et al., Biochem Pharmacol 2006 : In order to explore the mechanism responsible for the inhibition of iNOS gene expression by rengyolone, we investigated its effect on LPS induced nuclear factor-kappaB (NF-kappaB) activation ... The LPS induced DNA binding activity of NF-kappaB was significantly inhibited by rengyolone, and this effect was mediated through inhibition of the degradation of inhibitory factor-kappaBalpha and phosphorylation of p38 MAP kinase
Uesugi et al., Brain Res 2006 : These results suggest that both MKK3/6-p38MAPK and MKK4-JNK pathways are important signaling cascades leading to the induction of TNFalpha in LPS stimulated microglia, but that NFkappaB itself is not required for this induction ... These results suggest that both MKK3/6-p38MAPK and MKK4-JNK pathways are important signaling cascades leading to the induction of TNFalpha in LPS stimulated microglia, but that NFkappaB itself is not required for this induction
Calkin et al., Diabetologia 2006 (Atherosclerosis...) : In addition, gemfibrozil reduced the expression of the genes encoding nuclear factor kappa B (NF-kappaB) subunit, p65, the NF-kappaB dependent chemokine monocyte chemoattractant protein-1 , and tissue factor
Folmer et al., Biochem Pharmacol 2006 : In order to understand underlying regulatory mechanisms, inhibition of both NF-kappaB-driven reporter gene expression and TNFalpha induced binding of NF-kappaB to a consensus response element was achieved at concentrations of 320 microM ( flavokavain A ), 175 microM ( flavokavain B ) and 870 microM ( kavain and dihydrokavain )
Balzary et al., J Pharmacol Exp Ther 2006 : Lipopolysaccharide (LPS) , a Toll-like receptor (TLR) 4 agonist, causes airway hyperreactivity through nuclear factor-kappaB (NF-kappaB) ... Because NF-kappaB induces cyclooxygenase-2 (COX-2) to increase synthesis of prostaglandins (PGs), including the potent airway anti-inflammatory and smooth muscle relaxant PGE ( 2 ), we investigated whether LPS causes short-term PGE ( 2 ) -dependent relaxation of mouse isolated trachea
Packham et al., Cell Death Differ 1997 : Fas activates NF-kappaB and induces apoptosis in T-cell lines by signaling pathways distinct from those induced by TNF-alpha ... Surprisingly, Fas induced signaling also triggered the activation of NF-kappaB in T cells, yet the kinetics of NF-kappaB induction by Fas was markedly delayed ... NF-kappaB activation by both pathways was persistent and due to the sequential degradation of IkappaB-alpha and IkappaB-beta ... NF-kappaB activation by both pathways was persistent and due to the sequential degradation of IkappaB-alpha and IkappaB-beta
Laos et al., Int J Oncol 2006 (Colonic Neoplasms) : Overexpression of CD43 suppressed constitutive as well as PMA induced NF-kappaB activation and reduced the DNA binding of transcription factor p65 but not p50
Yang et al., Clin Cancer Res 2006 (Melanoma) : Inhibition of constitutive IKK activity by BMS-345541 resulted in the reduction of NF-kappaB activity, CXCL1 chemokine secretion by cultured melanoma cells and melanoma cell survival in vitro and in vivo
Syeda et al., J Biol Chem 2006 : Activation of PAR-1 or PAR-2 promoted nuclear translocation and phosphorylation of p65-NF-kappaB, and thrombin induced but not PAR-2 induced p65-NF-kappaB phosphorylation was reduced by inhibition of MEK or p38MAPK ... Activation of PAR-1 or PAR-2 promoted nuclear translocation and phosphorylation of p65-NF-kappaB, and thrombin induced but not PAR-2 induced p65-NF-kappaB phosphorylation was reduced by inhibition of MEK or p38MAPK ... Activation of PAR-1 or PAR-2 promoted nuclear translocation and phosphorylation of p65-NF-kappaB, and thrombin induced but not PAR-2 induced p65-NF-kappaB phosphorylation was reduced by inhibition of MEK or p38MAPK ... Activation of PAR-1 or PAR-2 promoted nuclear translocation and phosphorylation of p65-NF-kappaB, and thrombin induced but not PAR-2 induced p65-NF-kappaB phosphorylation was reduced by inhibition of MEK or p38MAPK ... Activation of PAR-4 by AYPGKF increased phosphorylation of ERK1/2 and p38MAPK without modifying NF-kappaB activation or COX-2 induction ... Our data show that PAR-1 and PAR-2, but not PAR-4, are coupled with COX-2 expression and sustained endothelial production of vasculoprotective prostacyclin by mechanisms that depend on ERK1/2, p38MAPK, and IkappaBalpha dependent NF-kappaB activation
Fan et al., Rheumatol Int 2006 : Role of mitogen activated protein kinases and NFkappaB on IL-1beta induced effects on collagen type II, MMP-1 and 13 mRNA expression in normal articular human chondrocytes ... Role of mitogen activated protein kinases and NFkappaB on IL-1beta induced effects on collagen type II, MMP-1 and 13 mRNA expression in normal articular human chondrocytes ... Inhibitors of ERK and NFkappaB could significantly block the induction of MMP-1 and -13 ( p < 0.05 ) and the repression of collagen type II ( p < 0.01 ) ... Inhibitors of ERK and NFkappaB could significantly block the induction of MMP-1 and -13 ( p < 0.05 ) and the repression of collagen type II ( p < 0.01 )
Chang et al., Cell 2006 (Liver Failure, Acute) : Here we show that TNFalpha mediated JNK activation accelerates turnover of the NF-kappaB induced antiapoptotic protein c-FLIP , an inhibitor of caspase-8
Perez-Moreno et al., Cell 2006 (Dermatitis...) : Both in vivo and in vitro, p120 null epidermal cells activate nuclear NFkB , triggering a cascade of proinflammatory NFkB targets ... Although the underlying mechanism is likely complex, we show that p120 affects NFkB activation and immune homeostasis in part through regulation of Rho GTPases
Xu et al., J Biol Chem 2006 (Burkitt Lymphoma) : LMP-1 mediated activation of NF-kappaB is apparently necessary and sufficient for LMP-1 mediated priming effect in DG75 cells, a human B cell line
Yan et al., Braz J Med Biol Res 2006 : In this study, we found that Pim-1 inhibited the Elk-1 and NFkappaB transcriptional activities induced by activation of the mitogen activated protein kinase cascade in reporter gene assays ... These data indicate that Pim-1 does not regulate the activation of Erk1/2, Elk-1 or NFkappaB
Ye et al., Am J Physiol Gastrointest Liver Physiol 2006 : The TNF-alpha induced increase in MLCK promoter activity was mediated by NF-kappaB activation, and the inhibition of NF-kappaB activation prevented the TNF-alpha induced increase in promoter activity and the subsequent increase in MLCK protein expression and Caco-2 TJ permeability ... NF-kappaB p50/p65 activation of the MLCK promoter then leads to a stepwise increase in MLCK transcription, expression and activity, and MLCK mediated opening of the intestinal TJ barrier
Kundu et al., Carcinogenesis 2006 : Resveratrol inhibits phorbol ester induced expression of COX-2 and activation of NF-kappaB in mouse skin by blocking IkappaB kinase activity ... Resveratrol inhibits phorbol ester induced expression of COX-2 and activation of NF-kappaB in mouse skin by blocking IkappaB kinase activity ... To get further insights into the molecular basis of NF-kappaB inactivation by resveratrol, we examined the role of IkappaB kinase (IKK) in mediating TPA induced activation of NF-kappaB and COX-2 expression
Seth et al., Cell Res 2006 : RIG-I activates NF-kappaB and IRFs through the recently identified adaptor protein MAVS, a CARD domain containing protein that resides in the mitochondrial membrane
Shuai et al., Cell Res 2006 : This article is to review the role of PIAS proteins in the regulation of STAT and NF-kB signaling pathways
Beers et al., Biochemistry 2006 : These results suggest that TNF alpha suppresses apoAI promoter activity through both the MEK/ERK and JNK pathways but is not mediated by either p38 MAP kinase activity or NF-kappaB activation ... These results suggest that TNF alpha suppresses apoAI promoter activity through both the MEK/ERK and JNK pathways but is not mediated by either p38 MAP kinase activity or NF-kappaB activation
Song et al., Proc Natl Acad Sci U S A 2006 : TES1- and TES2 mediated NF-kappaB activations are IL-1 receptor associated kinase 1 ( IRAK1 ) -dependent ... Because IRAK1 is upstream of TRAF6 in IL-1 activation of NF-kappaB, the potential role of IRAK1 in LMP1 mediated NF-kappaB activation through TRAF6 and inhibitor of kappaB (IkappaB) kinase ( IKK ) was initially investigated ... Because IRAK1 is upstream of TRAF6 in IL-1 activation of NF-kappaB , the potential role of IRAK1 in LMP1 mediated NF-kappaB activation through TRAF6 and inhibitor of kappaB (IkappaB) kinase ( IKK ) was initially investigated ... Because IRAK1 is upstream of TRAF6 in IL-1 activation of NF-kappaB, the potential role of IRAK1 in LMP1 mediated NF-kappaB activation through TRAF6 and inhibitor of kappaB (IkappaB) kinase ( IKK ) was initially investigated ... Because IRAK1 is upstream of TRAF6 in IL-1 activation of NF-kappaB, the potential role of IRAK1 in LMP1 mediated NF-kappaB activation through TRAF6 and inhibitor of kappaB (IkappaB) kinase ( IKK ) was initially investigated ... Because IRAK1 is upstream of TRAF6 in IL-1 activation of NF-kappaB, the potential role of IRAK1 in LMP1 mediated NF-kappaB activation through TRAF6 and inhibitor of kappaB (IkappaB) kinase ( IKK ) was initially investigated ... Because IRAK1 is upstream of TRAF6 in IL-1 activation of NF-kappaB, the potential role of IRAK1 in LMP1 mediated NF-kappaB activation through TRAF6 and inhibitor of kappaB (IkappaB) kinase ( IKK ) was initially investigated ... Further, LMP1 TES1 and TES2 induced p65, p50, and p52 NF-kappaB DNA binding in WT and IRAK1-deficient I1A 293 cells ... Thus, the critical IRAK1 role in LMP1 induced NF-kappaB activation is in mediating p65/RelA S536 phosphorylation through an effect on p38 or other p65 S536 kinases
Chen et al., Zhonghua Shao Shang Za Zhi 2005 (Burns) : Burn serum can enhance the expression of VCAM-1 in HUVECs through p38 MAPK signaling pathway, and the activation of NF-kappaB was also involved in this process
Phan et al., Gene 2006 (Burns...) : CD14 dependent modulation of NF-kappaB alternative splicing in the lung after burn injury
Li et al., Biochem Biophys Res Commun 2006 : Overexpression of ABIN-2 , a negative regulator of NF-kappaB , delays liver regeneration in the ABIN-2 transgenic mice ... Our transgenic data demonstrated that overexpression of ABIN-2 inhibited NF-kappaB nuclear translocation, which peaked at around 2-4h post-hepatectomy, and this led to an impairment of the G1/S transition as well as a delay in hepatocyte cell cycle progression of the regenerating liver
Schmidt-Ullrich et al., Development 2006 : Furthermore, the similarity between the phenotypes of c ( IkappaBADeltaN ) mice and mice deficient in Eda A1 ( tabby ) or its receptor EdaR ( downless ) raised the issue of whether in vivo NF-kappaB regulates or is regulated by these novel TNF family members ... We show that NF-kappaB activation is essential for induction of Shh and cyclin D1 expression and subsequent placode down growth ... We show that NF-kappaB activation is essential for induction of Shh and cyclin D1 expression and subsequent placode down growth
Rydkina et al., Ann N Y Acad Sci 2005 : Our data also suggest that p38 activity does not contribute to NF-kappaB response, implicating involvement of independent upstream signaling pathways
Zhao et al., J Biol Chem 2006 : IFN-gamma enhanced lipopolysaccharide (LPS) induced ERK and JNK phosphorylation but had no effect on LPS induced NF-kappaB activation
Spangenburg et al., J Physiol 2006 : SOCS-3 overexpression increased NF-kappaB transcriptional activity by 27-fold
Ruiz et al., J Nutr 2006 (Inflammation) : We demonstrated that 3'-hydroxy-flavone but not the chemical core structure flavone blocked TNF-alpha induced nuclear factor (NF)-kappaB transcriptional activity and IP-10 expression at the level of NF-kappaB/IkappaBalpha phosphorylation/degradation by inhibiting IkappaB kinase activity ... In addition to the compound-specific inhibition of TNF induced NF-kappaB DNA binding and NF-kappaB transcriptional activity, apigenin and luteolin selectively blocked Akt phosphorylation/activity
Chen et al., Arterioscler Thromb Vasc Biol 2006 : The critical role of NF-kappaB in Ang II-induced LOX-1 promoter activation was confirmed by mutagenesis assay, and further confirmed by blocking NF-kappaB activation with the NF-kappaB inhibitor caffeic acid phenethyl ester or NF-kappaB p65 siRNA ... The critical role of NF-kappaB in Ang II-induced LOX-1 promoter activation was confirmed by mutagenesis assay, and further confirmed by blocking NF-kappaB activation with the NF-kappaB inhibitor caffeic acid phenethyl ester or NF-kappaB p65 siRNA
Belcher et al., J Clin Invest 2006 (Anemia, Sickle Cell...) : Treatment of sickle mice with hemin further increases heme oxygenase-1 expression and inhibits hypoxia/reoxygenation induced stasis, leukocyte-endothelium interactions, and NF-kappaB , VCAM-1, and ICAM-1 expression
Ivanov et al., Exp Cell Res 2006 (Melanoma) : We report here the use of a combination of sodium arsenite, an inhibitor of NF-kappaB activation, and NS398, a cyclooxygenase-2 (COX-2) inhibitor , for restoration of the surface FasL expression
Pokharel et al., Nitric Oxide 2006 : It is known that NF-kappaB activation is controlled by the phosphorylation and subsequent degradation of I-kappaBalpha , and in the present study, we found that the phosphorylation and degradation of I-kappaBalpha were also inhibited by taiwaniaflavone
Popov et al., J Biol Chem 2006 (Liver Cirrhosis...) : p38 mitogen activated protein kinase ( p38 MAPK ) and nuclear factor kappaB(NFkappaB) pathways were activated by HAL, and specific inhibitors of p38 MAPK and NFkappaB dose dependently inhibited MMP-13 induction
Ji et al., World J Gastroenterol 2006 (Carcinoma, Hepatocellular...) : NF-kappaB may cause activation and production of TGF-beta1
Li et al., Acta Pharmacol Sin 2006 (Pulmonary Disease, Chronic Obstructive) : Similarly, NAC inhibited the activation of NF-kB induced by TNF- alpha, but had no effect on the activation of NF-kappaB induced by IL-1 ... Similarly, NAC inhibited the activation of NF-kB induced by TNF- alpha , but had no effect on the activation of NF-kappaB induced by IL-1 ... Similarly, NAC inhibited the activation of NF-kB induced by TNF- alpha, but had no effect on the activation of NF-kappaB induced by IL-1 ... PDTC significantly inhibited the activation of NF-kappa B induced by TNF- alpha and IL-1 ... PDTC significantly inhibited the activation of NF-kappa B induced by TNF- alpha and IL-1 ... The electrophoretic mobility shift assay also showed that PDTC and NAC do not directly inhibit NF-kappa B DNA binding activity in vitro
Anuar et al., Br J Pharmacol 2006 (Shock, Septic) : Nitroflurbiprofen ( 3-30 mg kg ( -1 ), i.p. ) administration resulted in a dose dependent inhibition of the LPS mediated increase in plasma TNF-alpha, IL-1beta and NO ( x ) concentration, liver H2S synthesis ( 55.00+/-0.95 nmole mg protein ( -1 ), c.f. 62.38+/-0.47 nmole mg protein ( -1 ), n = 5, P < 0.05 ), CSE mRNA, iNOS, MPO activity and NF-kappaB activation
Gerbod-Giannone et al., Proc Natl Acad Sci U S A 2006 : TNFalpha induces ABCA1 through NF-kappaB in macrophages and in phagocytes ingesting apoptotic cells ... TNFalpha induces ABCA1 through NF-kappaB in macrophages and in phagocytes ingesting apoptotic cells
Takada et al., J Immunol 2006 : It also abrogated NF-kappaB activation induced by TNF , IL-1beta, okadaic acid, doxorubicin, LPS, H2O2, PMA, and cigarette smoke ... It also abrogated NF-kappaB activation induced by TNF, IL-1beta , okadaic acid, doxorubicin, LPS, H2O2, PMA, and cigarette smoke ... It also abrogated NF-kappaB activation induced by TNF, IL-1beta, okadaic acid, doxorubicin, LPS , H2O2, PMA, and cigarette smoke ... AKBA did not directly affect the binding of NF-kappaB to the DNA but inhibited sequentially the TNF induced activation of IkappaBalpha kinase (IKK), IkappaBalpha phosphorylation, IkappaBalpha ubiquitination, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation ... AKBA did not directly affect the binding of NF-kappaB to the DNA but inhibited sequentially the TNF induced activation of IkappaBalpha kinase (IKK), IkappaBalpha phosphorylation, IkappaBalpha ubiquitination, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation ... AKBA did not directly affect the binding of NF-kappaB to the DNA but inhibited sequentially the TNF induced activation of IkappaBalpha kinase (IKK), IkappaBalpha phosphorylation, IkappaBalpha ubiquitination, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation ... AKBA did not directly affect the binding of NF-kappaB to the DNA but inhibited sequentially the TNF induced activation of IkappaBalpha kinase (IKK) , IkappaBalpha phosphorylation, IkappaBalpha ubiquitination, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation
Neumann et al., Biol Chem 2006 : Furthermore, we show that mitogen activated protein kinase kinase kinases ( MAP3K ), like TPL2 ( tumour progression locus 2 ) and transforming growth factor beta activated kinase 1 ( TAK1 ), have no impact on H. pylori induced activation of NF-kappaB
Kimura et al., Life Sci 2006 (Myocardial Ischemia) : TNF alpha production appeared to be regulated possibly at the post-transcriptional step by ribosomal S6 phosphorylation given that IP did not suppress TNF alpha mRNA up-regulation and was independent of NFkappaB activation
Fiers et al., FEBS Lett 1991 (Autoimmune Diseases...) : Furthermore, TNF induces a set of genes and at least part of this transcriptional activation is mediated by NF kappa B
Miao et al., Mol Endocrinol 2006 : Here, we show that CARM1 can act as a coactivator for the transcription factor nuclear factor-kappaB (NF-kappaB) and enhance NF-kappaB activity in a CBP ( p300 ) -dependent manner
Vilar et al., EMBO J 2006 : In PC12 cells, Bex1 overexpression inhibited the induction of NF-kappaB activity by NGF without affecting activation of Erk1/2 and AKT, while Bex1 knockdown accelerated neuronal differentiation and potentiated NF-kappaB activity in response to NGF ... In PC12 cells, Bex1 overexpression inhibited the induction of NF-kappaB activity by NGF without affecting activation of Erk1/2 and AKT, while Bex1 knockdown accelerated neuronal differentiation and potentiated NF-kappaB activity in response to NGF
Lee et al., EMBO J 2006 : In contrast, CD95 ligand stimulation of cells unable to internalize CD95 results in activation of proliferative Erk and NF-kappaB signaling pathways
Huang et al., Cell Death Differ 2006 : Osteoclast differentiation requires TAK1 and MKK6 for NFATc1 induction and NF-kappaB transactivation by RANKL ... Osteoclast differentiation requires TAK1 and MKK6 for NFATc1 induction and NF-kappaB transactivation by RANKL ... Osteoclast differentiation requires TAK1 and MKK6 for NFATc1 induction and NF-kappaB transactivation by RANKL ... TAK1-DN , MKK6-DN, and SB203580, but not MKK3-DN, also suppressed RANKL stimulation of NF-kappaB transcription activity in a manner dependent on p65 phosphorylation on Ser-536
Chanchevalap et al., Nucleic Acids Res 2006 : Importantly, suppression of KLF5 by small interfering RNA ( siRNA ) resulted in a reduction in p50 and p65 subunit mRNA levels and NF-kappaB DNA binding activity in response to LPS ... Importantly, suppression of KLF5 by small interfering RNA ( siRNA ) resulted in a reduction in p50 and p65 subunit mRNA levels and NF-kappaB DNA binding activity in response to LPS
Harashima et al., Biochem J 2006 (Diabetes Mellitus, Experimental...) : A transfection experiment revealed that the mRNA was actually translated as deduced to yield the secretory protein working as a decoy in AGE induced NF-kappaB ( nuclear factor kappaB ) activation
Waldow et al., Nitric Oxide 2006 : The TNFalpha induced expression of CAM was exclusively dependent on the transcription factor NFkappaB since the inhibitor of NFkappaB activation, BAY 11-7082, completely blocked the induction ... In summary, a short preincubation of HUVEC with SNAP/Cys results in a persistent suppression of NFkappaB dependent expression of CAM
Konstantinopoulos et al., Int J Colorectal Dis 2007 (Adenocarcinoma...) : Down-regulation of PPAR gamma and induction of the CBP transcriptional coactivator can augment NF-kappaB and AP-1 transcriptional activities leading to up-regulation of COX-2 expression in colon adenocarcinoma cells
Sun et al., Am J Physiol Endocrinol Metab 2006 : Taken together, these data suggest that VDR plays an inhibitory role in the regulation of NF-kappaB activation
Matsuyama et al., Am J Pathol 2006 (Pulmonary Fibrosis) : In IPF patients, DDR1 activation in fibroblasts inhibited Fas ligand induced apoptosis and resulted in NF-kappaB nuclear translocation
Narayan et al., Mol Cell Biol 2006 : CARMA1 is required for Akt mediated NF-kappaB activation in T cells ... We previously demonstrated a role for the serine/threonine kinase Akt in CD28 mediated NF-kappaB induction ... We previously demonstrated a role for the serine/threonine kinase Akt in CD28 mediated NF-kappaB induction ... Using a CARMA1-deficient T-cell line, we have now found that the CARMA complex is required for induction of NF-kappaB by Akt , in cooperation with protein kinase C activation ... Furthermore, using a novel selective inhibitor of Akt, we confirm that Akt plays a modulatory role in NF-kappaB induction by the TCR and CD28
Hsieh et al., J Cell Physiol 2006 : Moreover, S1P stimulated activation of NF-kappaB promoter activity was blocked by phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 and helenalin, but not by U0126, suggesting that involvement of PI3K/Akt in the activation of NF-kappaB ... Moreover, S1P stimulated activation of NF-kappaB promoter activity was blocked by phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 and helenalin, but not by U0126, suggesting that involvement of PI3K/Akt in the activation of NF-kappaB ... Moreover, S1P stimulated activation of NF-kappaB promoter activity was blocked by phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 and helenalin, but not by U0126, suggesting that involvement of PI3K/Akt in the activation of NF-kappaB
Wang et al., Cancer Res 2006 (Neoplasm Invasiveness...) : In our earlier report, we showed that down-regulation of Notch-1 reduced nuclear factor-kappaB (NF-kappaB) DNA binding activity and matrix metalloproteinase-9 (MMP-9) expression ... Consistent with these results, we found that the down-regulation of Notch-1 reduced NF-kappaB DNA binding activity and VEGF expression ... Because NF-kappaB, VEGF, and MMPs are critically involved in the processes of tumor cell invasion and metastasis, we investigated the role and mechanism ( s ) by which Notch-1 down-regulation ( using molecular approaches ) may lead to the down-regulation of NF-kappaB , vascular endothelial growth factor ( VEGF ), and MMP-9, thereby inhibiting invasion of pancreatic cancer cells through Matrigel
Al-Rasheed et al., J Am Soc Nephrol 2006 (Diabetic Nephropathies) : Phosphatidylinositol 3-kinase but not extracellular signal regulated mitogen activated protein kinase mediated C-peptide and insulin activation of NF-kappaB ... Phosphatidylinositol 3-kinase but not extracellular signal regulated mitogen activated protein kinase mediated C-peptide and insulin activation of NF-kappaB
Zhou et al., Br J Haematol 2006 (Lymphoma, B-Cell, Marginal Zone...) : t ( 11 ; 18 ) ( q21 ; q21 ) occurs specifically in mucosa associated lymphoid tissue ( MALT ) lymphoma and the translocation generates a functional API2-MALT1 fusion product that activates nuclear factor (NF)kappaB ... t ( 11 ; 18 ) ( q21 ; q21 ) occurs specifically in mucosa associated lymphoid tissue ( MALT ) lymphoma and the translocation generates a functional API2-MALT1 fusion product that activates nuclear factor (NF)kappaB
Hochart et al., Br J Haematol 2006 (Bacterial Infections...) : Proinflammatory cytokines tumour necrosis factor (TNF)-alpha, interleukin (IL)-8, IL-6 and IL-1beta release were subsequently measured by enzyme linked immunosorbent assay at 6 h, and nuclear translocation of the proinflammatory NF-kappaB was assessed at 2 h. Treatment with pharmacological doses of LMWH and UFH significantly attenuated LPS induced production of TNF-alpha, IL-8, IL-6 and IL-1beta as well as NF-kappaB translocation ... Proinflammatory cytokines tumour necrosis factor (TNF)-alpha, interleukin (IL)-8, IL-6 and IL-1beta release were subsequently measured by enzyme linked immunosorbent assay at 6 h, and nuclear translocation of the proinflammatory NF-kappaB was assessed at 2 h. Treatment with pharmacological doses of LMWH and UFH significantly attenuated LPS induced production of TNF-alpha, IL-8, IL-6 and IL-1beta as well as NF-kappaB translocation
Tong et al., Respir Res 2006 : Deletion of NF-kappaB binding sites within VEGF promoter abolished HIMF induced VEGF expression in MLE-12 cells, suggesting that activation of NF-kappaB is essential for VEGF upregulation induced by HIMF ... In addition, HIMF strongly induced Akt phosphorylation, and suppression of Akt activation by specific inhibitors and dominant negative mutants for PI-3K, and IKK or IkappaBalpha blocked HIMF induced NF-kappaB activation and attenuated HIMF induced VEGF production ... In addition, HIMF strongly induced Akt phosphorylation, and suppression of Akt activation by specific inhibitors and dominant negative mutants for PI-3K, and IKK or IkappaBalpha blocked HIMF induced NF-kappaB activation and attenuated HIMF induced VEGF production
Totzke et al., J Biol Chem 2006 : Interestingly, IkappaB-zeta is localized in the nucleus where it aggregates in matrix associated deacetylase bodies, indicating that IkappaB-zeta regulates nuclear NF-kappaB activity rather than its nuclear translocation from the cytoplasm ... IkappaB-zeta expression itself was regulated by NF-kappaB , suggesting that its activity is controlled in a negative feedback loop
Chang et al., Mol Cancer Res 2006 (Leukemia, T-Cell) : Here, we provide evidence that sequential NF-kappaB activating signals induce heightened NF-kappaB DNA binding and p21 ( waf1/cip1 ) induction in CEM and additional T leukemic cell lines ... Subsequent stimulation of these cells with an anticancer agent, etoposide, results in augmented NF-kappaB dependent p21 ( waf1/cip1 ) induction and increased chemoresistance of the leukemia cells
Lin et al., Arterioscler Thromb Vasc Biol 2006 : Mechanistically, KLF2 inhibits PAR-1 expression and, as a consequence, thrombin mediated nuclear factor kappaB (NF-kappaB) nuclear accumulation and DNA binding ... Conversely, small interfering RNA mediated knockdown of KLF2 increases PAR-1 expression and thrombin mediated induction of NF-kappaB activation ... Conversely, small interfering RNA mediated knockdown of KLF2 increases PAR-1 expression and thrombin mediated induction of NF-kappaB activation
Kårehed et al., Mol Immunol 2007 : Our results suggest that IFN-gamma induced increase of FcgammaRI expression requires the integration of two signalling events : PKR dependent Stat1 serine 727 phosphorylation, and activation of NFkappaB ... Our results suggest that IFN-gamma induced increase of FcgammaRI expression requires the integration of two signalling events : PKR dependent Stat1 serine 727 phosphorylation, and activation of NFkappaB ... Our results suggest that IFN-gamma induced increase of FcgammaRI expression requires the integration of two signalling events : PKR dependent Stat1 serine 727 phosphorylation, and activation of NFkappaB
Bae et al., Biochim Biophys Acta 2006 : Taken together, our results suggest that NF-kappaB may play pivotal roles in GFAP gene expression ... To further delineate whether NF-kappaB is directly involved in the regulation of GFAP gene expression, we transfected A172 cells with an expression vector encoding a super-repressor IkappaBalpha protein ( IkappaBalpha-SR ) to specifically inhibit NF-kappaB activity and found the marked reduction of GFAP protein levels in IkappaBalpha-SR-transfectant cells
Pergola et al., Nitric Oxide 2006 : Inhibition of iNOS protein expression appeared to be at the transcriptional level, since the extract and similarly cyanidin-3-O-glucoside ( 10, 20, 40, and 80 microg/ml, amounts corresponding to the concentrations present in the extract ) decreased LPS induced NF-kappaB activation , through inhibition of IkappaBalpha degradation, and reduced ERK-1/2 phosphorylation in a concentration dependent manner
Folch-Puy et al., J Immunol 2006 : Pancreatitis associated protein I suppresses NF-kappa B activation through a JAK/STAT mediated mechanism in epithelial cells
Matsuo et al., Kidney Int 2006 (Fibrosis) : Although glucose increased phosphorylation of MEK1/2, p42/44 MAPK, p38 MAPK, JNK1/2, and PKC, and DNA binding activity of NF-kappaB, its effect on MCP-1 expression was suppressed only by PKC and NF-kappaB inhibitors ... Prednisolone increased I-kappaB-alpha expression and inhibited glucose/mannitol induced NF-kappaB DNA binding and MCP-1 expression without affecting PKC phosphorylation
Ishiguro et al., Cell Signal 2006 : RNA interference against Nore1B attenuates NF-kappaB activation induced by T cell receptor ( TCR ) ligation, but not NF-kappaB activation induced by TNFalpha or lipoteichoic acid
Kokkinakis et al., Cancer Lett 2006 (Neoplasms) : The response of tumors to MET-stress depends on their mutational status, however, it always involves inhibition of CDK1 and in most cases the upregulation of p21, p27, GADDs and 14-3-3sigma in response to upregulation of TGF-beta, IRF-1, TNF-alpha, Rb and/or MDA-7 and the downregulation of PI3K, RAS and NF-kappaB ... The response of tumors to MET-stress depends on their mutational status, however, it always involves inhibition of CDK1 and in most cases the upregulation of p21, p27, GADDs and 14-3-3sigma in response to upregulation of TGF-beta, IRF-1, TNF-alpha , Rb and/or MDA-7 and the downregulation of PI3K, RAS and NF-kappaB ... The response of tumors to MET-stress depends on their mutational status, however, it always involves inhibition of CDK1 and in most cases the upregulation of p21, p27, GADDs and 14-3-3sigma in response to upregulation of TGF-beta, IRF-1 , TNF-alpha, Rb and/or MDA-7 and the downregulation of PI3K, RAS and NF-kappaB
Won et al., Br J Pharmacol 2006 (Edema) : Buddlejasaponin IV inhibited the LPS induced activation of nuclear factor-kappaB (NF-kappaB) , a transcription factor necessary for proinflammatory mediators, iNOS, COX-2, TNF-alpha, IL-1beta and IL-6 expression
Shah et al., Eur J Cancer Prev 2006 (Colorectal Neoplasms) : Chronic c-9,t-11-CLA and t-10 , c-12-CLA treatment inhibited DCA induced PKC beta1 and PKC delta translocation and also inhibited NF-kappaB activation ... Chronic c-9,t-11-CLA and t-10, c-12-CLA treatment inhibited DCA induced PKC beta1 and PKC delta translocation and also inhibited NF-kappaB activation
Gao et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2006 : To elucidate the receptor mechanisms underlying the modulation of lipopolysaccharide (LPS) induced nuclear factor-kappaB (NF-kappaB) expression in human umbilical vein endothelial cell line ECV-304 cells by cholecystokinin octapeptide ( CCK-8 ) ... The inhibitory effects of CCK-8 on NF-kappaB p65 protein expression were attenuated by proglumide > CR-2945 > CR-1409 ... CCK-AR and CCK-BR are involved in the mediation of CCK-8 inhibitive regulation for LPS induced NF-kappaB protein expression in ECV-04 cells, whereas the effect of CCK-BR are more than that of CCK-R
Wen et al., J Pharmacol Exp Ther 2006 : IkappaB kinase (IKK) beta is essential for inflammatory cytokine induced activation of nuclear factor kappaB (NF-kappaB) ... IkappaB kinase (IKK) beta is essential for inflammatory cytokine induced activation of nuclear factor kappaB (NF-kappaB) ... Here, we report the mechanism and the effect of the IKKbeta inhibitor N- ( 6-chloro-7-methoxy-9H-beta-carbolin-8-yl ) -2-methylnicotinamide ( ML120B ), a beta-carboline derivative, on NF-kappaB signaling and gene activation in RA-relevant cell systems ... ML120B concentration-dependently inhibits tumor necrosis factor alpha (TNFalpha) stimulated NF-kappaB signaling via inhibition of IkappaBalpha phosphorylation, degradation, and NF-kappaB translocation into the nucleus
Horie et al., Leukemia 2006 (Leukemia, Lymphocytic, Chronic, B-Cell) : Dehydroxymethylepoxyquinomicin also inhibited NF-kappaB induced by CD40 and enhanced fludarabine mediated apoptosis of CLL cells
Huang et al., J Biomed Sci 2006 : Besides regulating lipid metabolism, peroxisome proliferator activated receptor ( PPAR)-alpha activators also reduce NF-kappaB activation to terminate activation of inflammatory pathways ... Here, we demonstrate that GP extract and one of its main components, Gypenoside XLIX ( Gyp-XLIX ) inhibited LPS induced NF-kappaB activation in murine macrophages ... Furthermore, Gyp-XLIX restored the LPS- and TNF-alpha induced decrease in cytosolic I-kappaBalpha protein expression and inhibited the translocation of NF-kappaB ( p65 ) to the nucleus in THP-1 monocyte and HUVEC cells ... The inhibition of LPS- and TNF-alpha induced NF-kappaB luciferase activity in macrophages was abolished by MK-886, a selective PPAR-alpha antagonist
Higuchi et al., Pharmazie 2006 (Drug-Induced Liver Injury) : The production of TNFalpha was significantly inhibited by cationic liposomes/NFkappaB decoy complex but not by cationic liposomes/random decoy complex or naked NFkappaB decoy
Suh et al., Arch Biochem Biophys 2006 : Transactivation of LPS stimulated NF-kappaB was inhibited by U0126 ... These results suggest that the transcription factor NF-kappaB is involved in ERK mediated iNOS regulation and that activation of the Ras/ERK pathway contributes to the induction of iNOS expression in RAW264.7 cells in response to LPS
Frey et al., J Biol Chem 2006 : Phosphatidylinositol 3-kinase gamma signaling through protein kinase Czeta induces NADPH oxidase mediated oxidant generation and NF-kappaB activation in endothelial cells ... Phosphatidylinositol 3-kinase gamma signaling through protein kinase Czeta induces NADPH oxidase mediated oxidant generation and NF-kappaB activation in endothelial cells ... Additionally, the TNFalpha activated NF-kappaB binding to the ICAM-1 promoter, ICAM-1 protein expression, and PMN adhesion to ECs required functional PI3Kgamma ... Thus, PI3Kgamma lies upstream of PKCzeta in the endothelium, and its activation is crucial in signaling NADPH oxidase dependent oxidant production and subsequent NF-kappaB activation and ICAM-1 expression
Arcaroli et al., Am J Respir Crit Care Med 2006 (Sepsis...) : The IL-1 receptor associated kinase ( IRAK-1 ) plays a central role in TLR2- and TLR4 induced activation of nuclear factor (NF)-kappaB , a critical event in the transcriptional regulation of many sepsis associated proinflammatory mediators ... The IL-1 receptor associated kinase ( IRAK-1 ) plays a central role in TLR2- and TLR4 induced activation of nuclear factor (NF)-kappaB , a critical event in the transcriptional regulation of many sepsis associated proinflammatory mediators
Sougioultzis et al., Biochem Biophys Res Commun 2006 : Saccharomyces boulardii produces a soluble anti-inflammatory factor that inhibits NF-kappaB mediated IL-8 gene expression ... The probiotic yeast Saccharomyces boulardii exerts an anti-inflammatory effect by producing a low molecular weight soluble factor that blocks NF-kappaB activation and NF-kappaB mediated IL-8 gene expression in intestinal epithelial cells and monocytes
Wang et al., Brain Res 2006 (Reperfusion Injury) : The purpose of this study is to investigate the role of nuclear factor kappa-B (NF-kappaB) in regulating IL-6 expression after ischemia
Feuillard et al., Cytokine 1991 (Lymphoma) : In the present work, we investigated the molecular events leading to NF-kappa B activation by TNF alpha in a human T cell line ( Jurkat ) and its subclone JCT6, which presents a deficiency in the PKA transduction pathway ... Thus, TNF alpha induced NF-kappa B activation was found to be mediated by none of the major signal mediating kinases such as protein kinase C ( PKC ), protein kinase A, or Ca ( 2+ ) -regulated kinases ... Furthermore, we found that cytoplasmic acidification facilitated NF-kappa B activation by both TNF alpha and PKC, by a mechanism that increases NF-kappa B/I kappa B dissociation without affecting the NF-kappa B translocation step
Singh et al., Apoptosis 2006 (Histiocytoma) : Inhibition of proteasome activity or its upstream PI3 kinase activity inhibited NFkappaB translocation thereby suppressing apoptosis, which highlights the requirement of NFkappaB activation for completion of apoptosis in AK-5 cells
Gutiérrez et al., Toxicology 2006 : CrEL induced no significant NF-kappaB activation, changes in p50 and p65 NF-kappaB subunits or induction of iNOS protein
Sun et al., J Biol Chem 2006 : Cells treated with tunicamycin, an inhibitor of glycosylation, prevented TLR3 induced NF-kappaB activation, confirming that N-linked glycosylation is required for bioactivity of this receptor
Hou et al., J Agric Food Chem 2006 : The anti-inflammatory effect of CL-bromelain was correlated with reduced LPS induced NF-kappaB activity and cyclooxygenase 2 (COX-2) mRNA expression in rat livers
Mbvundula et al., J Pharm Pharmacol 2006 : At 5 microM, Win-55,212-2 inhibited the expression of iNOS and COX-2 and activation of NF-kappaB ... At 5 microM, Win-55,212-2 inhibited the expression of iNOS and COX-2 and activation of NF-kappaB
Asano et al., J Pharm Pharmacol 2006 : Addition of meloxicam into synovial fibroblast cultures inhibited dose-dependently mRNA expression for MMPs and TIMPs, which were increased by TNF-alpha stimulation, through the suppression of NF-kappaB and AP-1 activation
Nencioni et al., Blood 2006 : As a suitable mechanism for these effects, bortezomib was found to down-regulate MyD88, an essential adaptor for TLR signaling, and to relieve LPS induced activation of NF-kappaB , IRF-3, and IRF-8 and of the MAP kinase pathway
Liu et al., Acta Pharmacol Sin 2006 (Drug-Induced Liver Injury) : Upregulation of TNF-alpha and IL-6 mRNA might be related to increases in LBP and CD14 mRNA expression and activation of NF-kappaB ... Upregulation of TNF-alpha and IL-6 mRNA might be related to increases in LBP and CD14 mRNA expression and activation of NF-kappaB
Wang et al., Cancer Res 2006 (Carcinoma...) : The secretory leukocyte protease inhibitor ( SLPI ) can attenuate the host proinflammatory response by blocking nuclear factor kappaB (NF-kappaB) mediated tumor necrosis factor alpha (TNF-alpha) production in macrophages
Misra et al., J Biol Chem 2006 (Prostatic Neoplasms) : The effects of alpha2M* on the NF-kappaB activation, antiapoptotic signaling, unfolded protein response signaling, and proapoptotic signaling were also reversed by this treatment
Li et al., J Biol Chem 2006 : Previous studies indicate that RIP plays an essential role for TNFalpha induced NF-kappaB activation, but the molecular mechanism by which RIP mediates TNFalpha signals to activate NF-kappaB is not fully defined ... However, it remains to be determined whether the ubiquitination of RIP is required for TNFalpha induced NF-kappaB activation ... Together, our studies provide the first genetic evidence that the ubiquitination of RIP is required for TNFalpha induced NF-kappaB activation
Frobøse et al., Mol Endocrinol 2006 : Although a great deal is known about the mechanism of activation of NFkappaB and MAPKs by IL-1 , much less is known about the down-regulation of this pathway ... Suppressor of cytokine signaling (SOCS)-3 was shown to inhibit IL-1 induced transcription and activation of NFkappaB and the MAPKs JNK and p38, but the mechanism is unknown ... Suppressor of cytokine signaling (SOCS)-3 was shown to inhibit IL-1 induced transcription and activation of NFkappaB and the MAPKs JNK and p38, but the mechanism is unknown ... Suppressor of cytokine signaling (SOCS)-3 was shown to inhibit IL-1 induced transcription and activation of NFkappaB and the MAPKs JNK and p38, but the mechanism is unknown
Bolick et al., Arterioscler Thromb Vasc Biol 2006 : Inhibition of rhoA and PKCalpha significantly reduced NF-kappaB activation, ICAM-1 induction , and monocyte adhesion to aorta ... Inhibition of rhoA and PKCalpha significantly reduced NF-kappaB activation, ICAM-1 induction , and monocyte adhesion to aorta ... Inhibition of rhoA and PKCalpha significantly reduced NF-kappaB activation, ICAM-1 induction, and monocyte adhesion to aorta
Medin et al., J Infect Dis 2006 (Dengue) : We studied the mechanisms of IL-8 induction, using luciferase reporter constructs, and the effect of pharmacological inhibitors of either IL-8 secretion or nuclear factor- kappa B (NF-kappa B) activation on IL-8 induction by dengue 2 virus ( DEN2V ) infection
Wang et al., Mol Cancer Ther 2006 (Pancreatic Neoplasms) : Because Notch-1 is known to cross-talk with another major cell growth and apoptotic regulatory pathway ( i.e., NF-kappaB ), we found that NF-kappaB is a downstream target of Notch because down-regulation of Notch reduced NF-kappaB activity
Herrin et al., J Immunol 2006 : In agreement with this finding, up-regulation of HSH2 expression in response to these stimuli is blocked by inhibitors of NF-kappaB activation and is potentiated by stimulation with PMA, suggesting that HSH2 expression is dependent on NF-kappaB activation
Paintlia et al., J Immunol 2006 (Demyelinating Diseases...) : IL-4 induced peroxisome proliferator activated receptor gamma activation inhibits NF-kappaB trans activation in central nervous system ( CNS ) glial cells and protects oligodendrocyte progenitors under neuroinflammatory disease conditions : implication for CNS demyelinating diseases
Wu et al., Nat Cell Biol 2006 : NEMO is essential for NF-kappaB activation, and NEMO dysfunction in humans is the cause of incontinentia pigmenti and hypohidrotic ectodermal dysplasia and immunodeficiency ( HED-ID ) ... Here, we show that NEMO binds to Lys 63- but not Lys 48-linked polyubiquitin, and that single point mutations in NEMO that prevent binding to Lys 63-linked polyubiquitin also abrogates the binding of NEMO to RIP in tumour necrosis factor (TNF)-alpha stimulated cells, the recruitment of IKK to TNF receptor (TNF-R) 1, and the activation of IKK and NF-kappaB ... Here, we show that NEMO binds to Lys 63- but not Lys 48-linked polyubiquitin, and that single point mutations in NEMO that prevent binding to Lys 63-linked polyubiquitin also abrogates the binding of NEMO to RIP in tumour necrosis factor (TNF)-alpha stimulated cells, the recruitment of IKK to TNF receptor (TNF-R) 1, and the activation of IKK and NF-kappaB
Heese et al., Cell Mol Immunol 2006 : NF-kappaB regulates B-cell derived nerve growth factor expression
Martinesi et al., Cell Biol Int 2006 : Neither apoptosis nor nuclear translocation of NF-kappaB , induced in HUVEC by TNF-alpha was influenced by 1,25 ( OH ) ( 2 ) D ( 3 ) treatment
Karagiannides et al., Proc Natl Acad Sci U S A 2006 (Colitis) : Moreover, human mesenteric preadipocytes transfected with a luciferase promoter/reporter system containing the IL-8 promoter with a mutated NF-kappaB site lost their ability to respond to SP, indicating that SP-induced IL-8 expression is NF-kappaB dependent
Marasa et al., Biochem J 2006 : The expression of TRPC1 induced by stable transfection of IEC-6 cells with the wild-type TRPC1 gene ( IEC-TRPC1 cells ) increased Ca2+ influx after Ca2+ store depletion and repressed NF-kappaB transactivation, which was associated with an increase in susceptibility to apoptosis induced by exposure to TNFalpha ( tumour necrosis factor-alpha ) plus CHX ( cycloheximide ) ( TNF-alpha/CHX ), or STS ( staurosporine ) ... Furthermore, inhibition of TRPC1 expression by transfection with siRNA ( small interfering RNA ) targeting TRPC1 ( siTRPC1 ) decreased Ca2+ influx, increased NF-kappaB transactivation, and prevented the increased susceptibility of IEC-TRPC1 cells to apoptosis
Citri et al., J Biol Chem 2006 : Focusing on human kinases, we have demonstrated that the chaperone recognizes a common surface in the amino-terminal lobe of kinases from diverse families, including two newly identified clients, NFkappaB inducing kinase and death associated protein kinase , and the oncoprotein HER2/ErbB-2
Zhao et al., J Cell Biochem 2006 (Colitis...) : In addition, ghrelin stimulated NF-kappaB binding activity and NF-kappaB p65 subunit phosphorylation, and induced IL-8 promoter activity and IL-8 protein secretion
Noh et al., Life Sci 2006 : Furthermore, this compound inhibited the level of LPS stimulated prostaglandin E2 ( PGE2 ) and LPS induced nuclear factor-kappa B (NF-kappaB)
Chandrasekar et al., J Biol Chem 2006 (Atherosclerosis) : IL-18 treatment increased both mRNA and protein expression of MMP9 in human coronary artery SMCs. Gel shift, enzyme linked immunosorbent, and chromatin immunoprecipitation assays revealed a strong induction of IL-18 mediated AP-1 ( c-Fos, c-Jun, and Fra-1 ) and NF-kappaB ( p50 and p65 ) activation and stimulation of MMP9 promoter dependent reporter gene activity in an AP-1- and NF-kappaB dependent manner
Kapasi et al., Nephron. Experimental nephrology 2006 : Interestingly, gp120 at a low dose not only induced NF-kappaB activation but also increased p21 ( cip1/waf1 ) andp27 ( kip1 ) protein levels
Jacobo-Herrera et al., J Nat Prod 2006 : Compound 2 also inhibited TNF-alpha induced NF-kappaB activation at 5 microM through the canonical pathway, but was inactive in the Tet-On-Luc assay, indicating specificity of action, although it interfered with Tet-On-Luc at higher concentrations
Homhual et al., J Nat Prod 2006 : Bruguierin A ( 1 ) also inhibited phorbol ester induced NFkappaB ( nuclear factor-kappaB ) luciferase activation with an IC ( 50 ) value of 1.4 microM and selectively inhibited cyclooxygenase-2 (COX-2) activity with an IC ( 50 ) value of 0.37 microM
Hennig et al., J Nutr Biochem 2006 (Inflammation) : Furthermore, inhibition of ERK activity by PD98059 and PI3K/Akt activity by LY294002 or wortmannin significantly reduced the LA-induced activation of nuclear factor kappa B (NF-kappaB) ... Furthermore, inhibition of ERK activity by PD98059 and PI3K/Akt activity by LY294002 or wortmannin significantly reduced the LA-induced activation of nuclear factor kappa B (NF-kappaB) ... Furthermore, inhibition of ERK activity by PD98059 and PI3K/Akt activity by LY294002 or wortmannin significantly reduced the LA-induced activation of nuclear factor kappa B (NF-kappaB)
Cho et al., Immunol Lett 2006 (Arthritis, Rheumatoid...) : TGF-beta1 blocked the degradation of cytosolic IkappaB-alpha and the translocation of activated NF-kappaB to the nucleus
Babu et al., J Biol Chem 2006 : Finally, Tpl2/Cot participates in the activation of NF-kappaB by Tax
Wang et al., Experimental biology and medicine (Maywood, N.J.) 2006 : The results showed that SbG stimulated the proliferation of cultured hepatocytes, possibly through NF-kappaB , but not of Toll-like receptor 4 activation ; whereas SbG-RAW-CM and SbG in trans-well significantly suppressed the proliferation of hepatocytes
Choi et al., Experimental biology and medicine (Maywood, N.J.) 2006 (Hyperoxia...) : These results indicate that hyperoxia induces myocardial tolerance against ischemia-reperfusion injury in association with Bcl-2 induction by NF-kappaB activation through reactive oxygen species and PKC dependent signaling pathway
Inoue et al., Proc Natl Acad Sci U S A 2006 (Arthritis, Experimental) : Detailed signaling studies showed that NF-kappaB also contributes to IL-6 production and that TNF-alpha induced NF-kappaB activation is MKK3 dependent ... Detailed signaling studies showed that NF-kappaB also contributes to IL-6 production and that TNF-alpha induced NF-kappaB activation is MKK3 dependent ... In contrast, LPS mediated activation of NF-kappaB does not require MKK3
Ray et al., Int Immunol 2006 (Shock, Septic) : Bandshift analysis revealed that LPS induced NF-kappaB binding activity to a functional site in the TNF-alpha promoter was significantly higher in Fos-/- than in wild-type macrophages
Terzidou et al., J Clin Endocrinol Metab 2006 : OTR promoter contains putative transcription factor binding sites for activating protein-1 (AP-1), CCAAT/enhancer binding protein (C/EBP), and nuclear factor-kappaB (NF-kappaB) , which may be activated by IL-1beta , whose concentrations increase with labor ... In transient transfection studies, OTR promoter activity was increased by C/EBPbeta and NF-kappaB , but not by AP-1 ... IL-1beta has a biphasic effect on OTR expression in myometrial cells, and C/EBP and NF-kappaB play synergistic roles in OTR promoter activation
Kang et al., FASEB J 2006 : TNF-alpha increased NF-kappaB expression and its activation, and dexamethasone partially reversed these effects ... The results indicate that TNF-alpha induced CD38 expression involves NF-kappaB expression and its activation and dexamethasone inhibits CD38 expression through NF-kappaB dependent and -independent mechanisms
Han et al., J Am Soc Nephrol 2006 (Diabetes Mellitus, Type 2...) : In addition, aldosterone induced upregulation of MCP-1 expression and NF-kappaB transcriptional activity in cultured cells, and spironolactone reduced both NF-kappaB activation and MCP-1 synthesis
Harding et al., Kidney Int 2006 : TGFbeta1 increased transcription by approximately 50 % and activated both AP-1 and NF-kappaB
Nakagawa et al., Leukemia 2006 (Lymphoma, B-Cell, Marginal Zone) : Several lines of evidence indicated that both BCL10 and MALT1 are required for nuclear factor kappa B (NF-kappaB) activation by antigen receptor stimulation in lymphocytes, and API2-MALT1 can bypass this BCL10/MALT1 signaling pathway ... Several lines of evidence indicated that both BCL10 and MALT1 are required for nuclear factor kappa B (NF-kappaB) activation by antigen receptor stimulation in lymphocytes, and API2-MALT1 can bypass this BCL10/MALT1 signaling pathway ... We recently demonstrated that API2-MALT1 can induce transactivation of the API2 gene through NF-kappaB activation, thus highlighting a positive feedback-loop mechanism of self-activation by upregulating its own expression in t ( 11 ; 18 ) MALT lymphomas ... We recently demonstrated that API2-MALT1 can induce transactivation of the API2 gene through NF-kappaB activation, thus highlighting a positive feedback-loop mechanism of self-activation by upregulating its own expression in t ( 11 ; 18 ) MALT lymphomas
Vig-Varga et al., Gynecol Oncol 2006 (Disease Models, Animal...) : In parallel to the growth inhibitory effect, ALA also affects a key inflammatory signaling pathway by inhibiting TNFalpha induced NF-kappaB signaling activity
Satoh et al., Am J Physiol Gastrointest Liver Physiol 2006 : Previously, we demonstrated that in vitro ethanol treatment of pancreatic acinar cells augmented the CCK-8 induced activation of NF-kappaB , a key signaling system involved in the inflammatory response of pancreatitis ... Dispersed rat pancreatic acini were treated with and without ethanol and then stimulated with CCK-8 ; 100 nM CCK-8 caused both NF-kappaB and PKC-delta, -epsilon, and -zeta activation, whereas 0.1 nM CCK-8 did not increase PKC-epsilon, PKC-zeta, or NF-kappaB activity ... Of note, stimulation of acini with ethanol and 0.1 nM CCK-8 resulted in the activation of PKC-delta, PKC-epsilon, and NF-kappaB ... These results suggest that ethanol enhances the CCK-8 induced NF-kappaB activation at least in part through its effects on PKC-epsilon
Zhi et al., J Cell Physiol 2006 (Inflammation) : Moreover, our data shows that SPHK1 is the isoform activated by TNFalpha, and plays an essential role on the TNFalpha triggered intracellular Ca2+ signals, degranulation, cytokine production, and activation of NFkappaB , thus suggesting a pivotal role for SPHK1 on the proinflammatory responses triggered by TNFalpha
Nakajima et al., Neurosci Lett 2006 : Taken together, these results suggest that the induction of IL-1beta and TNFalpha in endotoxin stimulated microglia is differentially regulated at the level of NFkappaB activation ... Taken together, these results suggest that the induction of IL-1beta and TNFalpha in endotoxin stimulated microglia is differentially regulated at the level of NFkappaB activation
O'Gorman et al., Cell Immunol 2005 : Signal transduction studies revealed that IL-1beta and TNF-alpha stimulation induced NFkappaB phosphorylation in A549 cells, but antagonist inhibition of NFkappaB p50-p65 phosphorylation correlated with marked reduction of IL-1beta or TNF-alpha induced CCL28 expression ... Signal transduction studies revealed that IL-1beta and TNF-alpha stimulation induced NFkappaB phosphorylation in A549 cells, but antagonist inhibition of NFkappaB p50-p65 phosphorylation correlated with marked reduction of IL-1beta or TNF-alpha induced CCL28 expression ... Signal transduction studies revealed that IL-1beta and TNF-alpha stimulation induced NFkappaB phosphorylation in A549 cells, but antagonist inhibition of NFkappaB p50-p65 phosphorylation correlated with marked reduction of IL-1beta or TNF-alpha induced CCL28 expression
Islam et al., Mol Cell Biol 2006 : Interleukin-1 (IL-1) stimulation of SP-A expression is mediated by NF-kappaB ( p65/p50 ) activation and increased binding to the TBE
Kaltschmidt et al., Mol Cell Biol 2006 (Synaptic Transmission) : Consequently, NF-kappaB inhibition led to a decrease in forskolin induced CREB phosphorylation
Choi et al., Mol Cell Biol 2006 (Carcinoma, Hepatocellular...) : We recently reported that NS5A protein interacts with TRAF2 and modulates tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB and Jun N-terminal protein kinase (JNK) ... Since NS5A and NS5B are the essential components of the HCV replication complex, we examined whether NS5B could modulate TNF-alpha induced NF-kappaB and JNK activation ... In this study, we have demonstrated that TNF-alpha induced NF-kappaB activation is inhibited by NS5B protein in HEK293 and hepatic cells ... Furthermore, NS5B protein inhibited both TRAF2- and IKK induced NF-kappaB activation
Hu et al., Mol Cell Biol 2006 (Breast Neoplasms...) : Autocrine tumor necrosis factor alpha links endoplasmic reticulum stress to the membrane death receptor pathway through IRE1alpha mediated NF-kappaB activation and down-regulation of TRAF2 expression ... ER stress induced NF-kappaB activation is impaired in IRE1alpha knockdown cells and IRE1alpha ( -/- ) MEFs ... We found, however, that inhibiting NF-kappaB significantly decreased ER stress induced cell death in a caspase-8 dependent manner ... Further studies suggest that ER stress induces down-regulation of TRAF2 expression, which impairs TNF-alpha induced activation of NF-kappaB and c-Jun N-terminal kinase and turns TNF-alpha from a weak to a powerful apoptosis inducer
Shan et al., Am J Physiol Lung Cell Mol Physiol 2006 (Asthma...) : Complementary studies further demonstrated that the ASM responses to LPS were associated with activation of the ERK1/2 and p38 MAPK signaling pathways, IKK mediated activation of NF-kappaB , and coupling of phosphorylated ERK1/2 with the p65 subunit of NF-kappaB ... Complementary studies further demonstrated that the ASM responses to LPS were associated with activation of the ERK1/2 and p38 MAPK signaling pathways, IKK mediated activation of NF-kappaB , and coupling of phosphorylated ERK1/2 with the p65 subunit of NF-kappaB ... Finally, activation of p38 MAPK with anisomycin prevented both the LPS induced stimulation of ERK1/2 mediated NF-kappaB activity and associated changes in ASM responsiveness ... Finally, activation of p38 MAPK with anisomycin prevented both the LPS induced stimulation of ERK1/2 mediated NF-kappaB activity and associated changes in ASM responsiveness ... Finally, activation of p38 MAPK with anisomycin prevented both the LPS induced stimulation of ERK1/2 mediated NF-kappaB activity and associated changes in ASM responsiveness
Mikenberg et al., Eur J Cell Biol 2006 : Tumor necrosis factor alpha (TNF-alpha) activates the degradation of IkappaB-alpha and the nuclear import of cytoplasmic NF-kappaB
Sánchez-Valdepeñas et al., J Immunol 2006 : Previous evidence suggested that NF-kappaB inducing kinase (NIK) might regulate IL-2 synthesis
Sanchez-Lockhart et al., J Immunol 2006 : CD28 signaling through PI3K results in the recruitment of protein kinase C (PKC)theta to the cSMAC, activation of NF-kappaB , and induction of IL-2 transcription ... CD28 signaling through PI3K results in the recruitment of protein kinase C (PKC)theta to the cSMAC, activation of NF-kappaB , and induction of IL-2 transcription ... We show that induction of IL-2 secretion under these conditions did not require expression of PKCtheta and did not induce NF-kappaB activation or IL-2 transcription
Sadikot et al., J Immunol 2006 (Cross Infection...) : In contrast, NF-kappaB inhibition reduced MIP-2 expression and neutrophil influx following P. aeruginosa infection
Sarkar et al., J Immunol 2006 : Receptor interacting protein-2 (RIP2) is a caspase recruitment domain ( CARD ) -containing kinase that interacts with caspase-1 and plays an important role in NF-kappaB activation ... This RIP2 induced NF-kappaB activity and caspase-1 binding was inhibited in a dose dependent fashion by ASC ... This RIP2 induced NF-kappaB activity and caspase-1 binding was inhibited in a dose dependent fashion by ASC ... These data suggest that ASC may direct caspase-1 away from RIP2 mediated NF-kappaB activation , toward caspase-1 mediated processing of proIL-1beta by interfering with the RIP2 caspase-1 interaction
Khera et al., Lab Invest 2006 : Culture of cells in the presence of 25 mM D-glucose ( i ) enhanced apoptosis stimulated by serum depletion, ( ii ) enhanced activation of caspase-3 , ( iii ) inhibited NF-kappaB activation, and ( iv ) decreased Bcl-2 : Bax ratio
Thimmulappa et al., J Clin Invest 2006 (Pneumonia...) : Nrf2 's regulation of cellular glutathione and other antioxidants is critical for optimal NF-kappaB activation in response to LPS and TNF-alpha ... Nrf2 's regulation of cellular glutathione and other antioxidants is critical for optimal NF-kappaB activation in response to LPS and TNF-alpha
Logani et al., Electromagnetic biology and medicine 2006 (Neoplasms) : The effect of millimeter waves ( MMWs ) on NF-kappaB activation induced by cyclophosphamide (CPA) was studied in the spleen of mice ... CPA , an anticancer drug, caused a marked increase ( 58.9-fold ) in NF-kappaB DNA binding activity as compared to the control group
Wang et al., Biol Pharm Bull 2006 (Carcinoma, Hepatocellular...) : Furthermore, electrophoretic mobility shift assay showed that after the cells were incubated with pitavastatin alone or with pitavastatin and TNF-alpha for 24 h, pitavastatin significantly decreased the DNA binding activity of NF-kappaB induced by TNF-alpha
Ishizawa et al., Biol Cell 2006 : xFADD and xRIP1 synergistically induced the activation of AP-1 and NF-kappaB , both of which were partially mediated by TRAF2 ( tumour-necrosis-factor-receptor associated factor 2 ) and TAK1 ( transforming-growth-factor-beta activated kinase 1 ) ... xFADD and xRIP1 synergistically induced the activation of AP-1 and NF-kappaB , both of which were partially mediated by TRAF2 ( tumour-necrosis-factor-receptor associated factor 2 ) and TAK1 ( transforming-growth-factor-beta activated kinase 1 )
Munhoz et al., J Neurosci 2006 (Chronic Disease...) : In this study, the effects of stress, GCs, and NMDA receptors on lipopolysaccharide (LPS) induced activation of NF-kappaB in the brain were investigated ... CUS potentiated the increase in LPS induced activation of NF-kappaB in frontal cortex and hippocampus but not in the hypothalamus ... Our results indicate that stress, via GC secretion, can increase LPS induced NF-kappaB activation in the frontal cortex and hippocampus, agreeing with a growing literature demonstrating proinflammatory effects of GCs
Li et al., Am J Physiol Cell Physiol 2006 : Subsequent investigation revealed that CGRP inhibited IL-1beta evoked NF-kappaB activity by suppressing IkappaBalpha phosphorylation and degradation ... Subsequent investigation revealed that CGRP inhibited IL-1beta evoked NF-kappaB activity by suppressing IkappaBalpha phosphorylation and degradation ... We previously showed that the CGRP inhibitory effect was mediated by elevated intracellular cAMP and show here that analogs of cAMP, 8-bromoadenosine 3',5'-cyclic monophosphothioate and the Sp isomer of adenosine 3',5'-cyclic monophosphothioate, mimicked the CGRP suppressive effect on IL-1beta induced ROS formation, NF-kappaB activation , and MCP-1 secretion ... We previously showed that the CGRP inhibitory effect was mediated by elevated intracellular cAMP and show here that analogs of cAMP, 8-bromoadenosine 3',5'-cyclic monophosphothioate and the Sp isomer of adenosine 3',5'-cyclic monophosphothioate, mimicked the CGRP suppressive effect on IL-1beta induced ROS formation, NF-kappaB activation, and MCP-1 secretion
Dragomir et al., Vascul Pharmacol 2006 : Together, the findings indicate that in endothelial cells aspirin and PPAR-alpha activators reduce the high glucose increased expression of MCP-1 by a mechanism that includes the inhibition of reactive oxygen species, and decrease of AP-1 and NF-kB activation
Vines et al., J Biol Chem 2006 : Overexpression of GSK-3beta did not affect the TNF-alpha induced nuclear translocation of NFkappaB but reduced the nuclear half-life of TNF-alpha induced NFkappaB considerably ( by as much as 9 h ) and enhanced phosphorylation ( by as much as 33 % ) ... Overexpression of GSK-3beta did not affect the TNF-alpha induced nuclear translocation of NFkappaB but reduced the nuclear half-life of TNF-alpha induced NFkappaB considerably ( by as much as 9 h ) and enhanced phosphorylation ( by as much as 33 % )
Blumenthal et al., Blood 2006 (Inflammation...) : The expression of WNT5A required Toll-like receptor signaling and NF-kappaB activation, which identifies a novel induction pathway for a Wingless homolog
Maezawa et al., Journal of neuroinflammation 2006 : Our results suggest that LPS activation of innate immune response in TR APOE glia results in opposing outcomes from microglia and astrocytes as a result of TR APOE dependent activation of p38MAPK or NF-kappaB signaling in these two cell types ... Our results suggest that LPS activation of innate immune response in TR APOE glia results in opposing outcomes from microglia and astrocytes as a result of TR APOE dependent activation of p38MAPK or NF-kappaB signaling in these two cell types
Ea et al., Mol Cell 2006 : The receptor interacting protein kinase 1 ( RIP1 ) is essential for the activation of nuclear factor kappaB (NF-kappaB) by tumor necrosis factor alpha (TNFalpha) ... Here, we present evidence that TNFalpha induces the polyubiquitination of RIP1 at Lys-377 and that this polyubiquitination is required for the activation of IkappaB kinase (IKK) and NF-kappaB
Ichikawa et al., Mol Cancer Res 2006 (Bone Resorption...) : Treatment of these cells with RANKL activated NF-kappaB, and coexposure of the cells to ACA completely suppressed RANKL induced NF-kappaB activation in a time- and concentration dependent manner ... These results indicate that ACA is an effective blocker of RANKL induced NF-kappaB activation and of osteoclastogenesis induced by RANKL and tumor cells, suggesting its potential as a therapeutic agent for osteoporosis and cancer associated bone loss
Devaraj et al., Am J Physiol Heart Circ Physiol 2006 : Preincubation with antibodies to CD32 and CD64 or transient transfection with small interference RNA to CD32 attenuated CRP induced NF-kappaB activity, ICAM, VCAM, and monocyte-endothelial cell adhesion under static conditions
Lee et al., Br J Pharmacol 2006 : 3. In our NF-kappaB luciferase reporter system, TNF-alpha induced NF-kappaB activation was observed to be reduced by rosmarinic acid ... In accordance with this result, rosmarinic acid also inhibited TNF-alpha induced phosphorylation and degradation of IkappaB-alpha, as well as nuclear translocation of NF-kappaB heterodimer induced by TNF-alpha
Chong et al., J Biomed Sci 2006 (Fibrosis...) : Thalidomide ( 100-800 nM ) concentration-dependently inhibited NFkappaB transcriptional activity induced by TNF-alpha , including IKKalpha expression and IkappaBalpha phosphorylation in HSC-T6 cells
Murakami et al., Biol Chem 2006 (Inflammation...) : ACA abrogated ERK1/2 and JNK1/2, but not p38 activation, as well as the activation and transcriptional activation of NF-kappaB and CREB, whereas nobiletin allowed phosphorylation of these MAPKs, while it suppressed AP-1, NF-kappaB, and CREB activation
Lotz et al., J Exp Med 2006 : Although the lipopolysaccharide (LPS) receptor complex TLR4/MD-2 was present in fetal, neonatal, and adult IECs, LPS induced nuclear factor kappaB (NF-kappaB) activation and chemokine ( macrophage inflammatory protein 2 [ MIP-2 ] ) secretion was only detected in fetal IECs ... Although the lipopolysaccharide (LPS) receptor complex TLR4/MD-2 was present in fetal, neonatal, and adult IECs, LPS induced nuclear factor kappaB (NF-kappaB) activation and chemokine ( macrophage inflammatory protein 2 [ MIP-2 ] ) secretion was only detected in fetal IECs
Gu et al., J Biol Chem 2006 (Inflammation) : In this study, we report that NF-kappaB activation by lipopolysaccharide and tumor necrosis factor-alpha plays a pivotal role in the suppression of cyp3a4 through interactions of NF-kappaB with the PXR.retinoid X receptor ( RXR ) complex
Jennings-Gee et al., J Biol Chem 2006 : Because NF-kappaB plays a role in cytokine dependent induction of MnSOD, IL-6 , and FH, we assessed the effect of E1A on NF-kappaB in cells treated with TNF ... TNF induced a quantitatively and temporally equivalent activation of NF-kappaB in control and E1A transfected cells
Hai et al., J Virol 2006 : Because IKKgamma-Delta protein is lacking a critical coiled-coil domain important in protein-protein interactions, we sought to determine its signaling properties by examining its ability to self associate, couple to activators of the canonical pathway, and mediate human T-cell leukemia virus type 1 ( HTLV-1 ) Tax induced NF-kappaB activity
Zheng et al., Mol Cell Biol 2006 : We found that TRADD is required for TNFR1 to induce NF-kappaB activation and caspase-8 dependent apoptosis but is dispensable for TNFR1 initiated, RIP1 dependent necrosis
Sunahori et al., Arthritis Res Ther 2006 (Arthritis, Rheumatoid) : NF-kappaB activation was induced in S100A8/A9 stimulated monocytes, but this activity was not inhibited by p38 MAPK inhibitors ... NF-kappaB activation was induced in S100A8/A9 stimulated monocytes, but this activity was not inhibited by p38 MAPK inhibitors
Zheng et al., J Biol Chem 2006 (Bone Resorption) : This response was time- and dose dependent, required NF-kappaB activation and new protein synthesis, and was specifically blocked by the RANKL decoy receptor osteoprotegerin
Gustin et al., J Biol Chem 2006 : Constitutively active Akt augments non-canonical NF-kappaB activity, whereas kinase dead Akt or inhibition of phosphatidylinositol 3-kinase have the opposite effect ... These observations show that Akt plays a role in activation of basal and induced non-canonical NF-kappaB activity
Lee et al., J Nutr 2006 (Edema) : Cocoa polyphenols inhibit phorbol ester induced superoxide anion formation in cultured HL-60 cells and expression of cyclooxygenase-2 and activation of NF-kappaB and MAPKs in mouse skin in vivo
Konturek et al., Dig Dis Sci 2006 (Adenocarcinoma...) : We conclude that ( 1 ) distal CRC patients show significantly higher serum progastrin levels than matched healthy controls, confirming that this hormone may be implicated in rectal carcinogenesis ; ( 2 ) CRC patients exhibit significantly higher serum levels of IL-8 and TNF-alpha than healthy controls, probably reflecting more widespread inflammatory reaction in the colonic mucosa in CRC ; ( 3 ) gastrin, COX-2, Bcl-2, survivin, and NFkappa B were overexpressed in CRC tumor compared to intact mucosa, but treatment with CLX significantly reduced serum levels of progastrin and IL-8 and TNF-alpha, which could mediate the up-regulation of COX-2 in CRC ; and ( 4 ) CLX also enhanced expression of COX-2 , while inhibiting the expression of gastrin, Bcl-2, survivin, and NFkappa B , suggesting that COX-2 inhibition might be useful in chemoprevention against CRC, possibly due to suppression of the antiapoptotic proteins and reduction in progastrin induced and NFkappa B-promoted tumor growth
Feng et al., J Surg Res 2006 (Respiratory Distress Syndrome, Adult...) : HES 130/0.4 can inhibit CLP induced PCP by attenuating pulmonary inflammation and NF-kappaB activation in vivo
Mohan et al., J Biol Chem 2006 (Colonic Neoplasms) : The upstream activation of caspase-2 is not dependent on its antioxidant property or NF-kappaB inhibition
Das et al., Proc Natl Acad Sci U S A 2006 (Atherosclerosis...) : Mechanistically, KLF2 inhibits the transcriptional activity of both NF-kappaB and activator protein 1, in part by means of recruitment of transcriptional coactivator p300/CBP associated factor
Lamkanfi et al., J Cell Biol 2006 : Caspases leave the beaten track : caspase mediated activation of NF-kappaB
Paik et al., Lab Invest 2006 (Fibrosis...) : TNF-alpha and IL-1beta significantly upregulated the expression of TLR2 mRNA and protein in HSCs. PGN and LTA induced NF-kappaB activation and stimulated production of IL-8 in HSCs. Pretreatment with TNF-alpha or IL-1beta augmented NF-kappaB activation and IL-8 production in response to PGN or LTA ... TNF-alpha and IL-1beta significantly upregulated the expression of TLR2 mRNA and protein in HSCs. PGN and LTA induced NF-kappaB activation and stimulated production of IL-8 in HSCs. Pretreatment with TNF-alpha or IL-1beta augmented NF-kappaB activation and IL-8 production in response to PGN or LTA ... Both PGN- and LTA induced NF-kappaB activation and IL-8 secretion were completely inhibited by anti-TLR2 blocking antibody ( T2.5 )
Lee et al., Eur J Immunol 2006 : Direct role of NF-kappaB activation in Toll-like receptor triggered HLA-DRA expression ... Additionally, nuclear factor (NF)-kappaB activation is required for the CpG-DNA activated and LPS activated HLA-DRA expression, whereas IFN-gamma induced MHC-II expression depends on CIITA rather than on NF-kappaB ... Comprehensive mutant analyses, electrophoretic mobility shift assays and chromatin immunoprecipitation assays, reveal that the functional interaction of NF-kappaB with the promoter element is necessary for the TLR mediated HLA-DRA induction by CpG-DNA and LPS
Karimi et al., Respir Res 2006 : In addition, NFkappaB-inhibitor blocked cigarette smoke induced IL-8 production
Vij et al., J Biol Chem 2006 (Cystic Fibrosis) : Inhibition of the proteasome with bortezomib ( PS-341/Velcade ) also rescued CFTR, but with less efficiency, and suppressed NFkappaB mediated IL8 activation ... The inhibition of the major stress-inducible transcription factor CHOP ( CCAAT/enhancer binding protein homologous protein ) /GADD153 together with bortezomib was most effective in repressing NFkappaB mediated IL8 activation compared with interference of VCP, MLN-273 ( proteasome inhibitor ), or 4-phenylbutyrate ( histone deacetylase inhibitor )
Khan et al., Infect Immun 2006 (Colitis...) : We demonstrated that C. rodentium activates TLR4 and rapidly induced NF-kappaB nuclear translocation in host cells in a partially TLR4 dependent manner
Fujimura et al., Infect Immun 2006 : RANKL induced phosphorylation of ERK, p38, and NF-kappaB was not suppressed by HbR, but that of Akt was markedly suppressed
Hu et al., Infect Immun 2006 : In addition, Campylobacter infected DCs triggered activation of NF-kappaB and significantly stimulated production of interleukin-1beta (IL-1beta), IL-6, IL-8, IL-10, IL-12, gamma interferon, and tumor necrosis factor alpha (TNF-alpha) compared to uninfected DCs
Delgado et al., Infect Immun 2006 (Cystic Fibrosis...) : IL-8 secretion was not due to an increase in NF-kappaB- or activator protein-1 dependent IL-8 promoter transcription, but instead depended on p38 and Erk mitogen activated protein kinases
Sandur et al., J Biol Chem 2006 (Inflammation) : We found that plumbagin inhibited NF-kappaB activation induced by TNF , and other carcinogens and inflammatory stimuli ( e.g. phorbol 12-myristate 13-acetate, H2O2, cigarette smoke condensate, interleukin-1beta, lipopolysaccharide, and okadaic acid )
Zhou et al., Acta Pharmacol Sin 2006 : Effects of adenoviral gene transfer of mutated IkappaBalpha , a novel inhibitor of NF-kappaB , on human monocyte derived dendritic cells ... To investigate the effects of adenoviral gene transfer of IkappaBalpha mutant ( IkappaBalphaM ), a novel inhibitor of nuclear factor kappaB (NF-kappaB) , on apoptosis, phenotype and function of human monocyte derived dendritic cells ( DC ) ... The transcription and expression of the IkappaBalphaM gene, and the inhibitory effect of IkappaBalphaM on tumor necrosis factor (TNF)-alpha induced NF-kappaB activation in mature DC were detected by polymerase chain reaction ( PCR ), reverse transcription-polymerase chain reaction ( RT-PCR ), Western blot analysis, and electrophoretic mobility shift assays, respectively ... Furthermore, AdIkappaBalphaM significantly suppressed the TNF-alpha induced NF-kappaB activation, augmented apoptosis, downregulated CD80, CD83, and CD86 surface molecules, IL-12 secretion levels and the ability to stimulate the proliferation of T cells in mature DC
Chu et al., Cancer Biol Ther 2006 (Carcinoma, Hepatocellular...) : In conclusion : CYLD inhibits TRAIL mediated NF-kappaB activation and enhances the sensitivity of HCC cells to TRAIL triggered apoptosis ... In conclusion : CYLD inhibits TRAIL mediated NF-kappaB activation and enhances the sensitivity of HCC cells to TRAIL triggered apoptosis
Dai et al., J Invest Dermatol 2006 : However, SOCS1 did not affect NF-kappaB signaling ... However, SOCS1 did not affect NF-kappaB signaling
Yokoyama et al., Liver Int 2006 (Bile Duct Diseases) : Stimulation of HIBECs with LPS resulted in translocation of NF-kappaB subunits from the cytoplasmic to the nuclear fraction, followed by increased secretion of a variety of chemokines/cytokines, including interleukin-8 (IL-8), monocyte chemotactic protein-1 (MCP-1), and IL-6
Zhou et al., Biochem Biophys Res Commun 2006 : HGF significantly promoted the expression of Bcl-2 and NF-kappaB , while decreasing the activities of caspase-3 and -9 without affecting Bax level
Ahn et al., Eur J Pharmacol 2006 : Platycodin D could activate inhibitor of nuclear factor-kappaB kinase ( IKK)-beta in the nuclear factor-kappaB (NF-kappaB) activation of upstream level, but not IKK-alpha
Hao et al., FEBS Lett 2006 : Mutants of ITK that lack kinase activity or a kinase domain can rescue cells lacking Tec family kinases for antigen receptor induced SRF activation, but not for NFAT, AP-1 or NFkappaB activation
Kim et al., Free Radic Biol Med 2006 (Bone Resorption) : Specifically, alpha-LA reduced DNA binding of NF-kappaB but did not inhibit IKK activation
Kuwahara et al., Am J Physiol Lung Cell Mol Physiol 2006 : We conclude that NE increases IL-8 transcription through p38/NF-kappaB activation via EGFR transactivation
Papoutsopoulou et al., Nat Immunol 2006 : In contrast, ABIN-2 deficiency did not affect agonist induced regulation of transcription factor NF-kappaB
Gunella et al., Br J Pharmacol 2006 (Pulmonary Fibrosis...) : MSP activates the transcription factor NF-kappaB ; this effect is more potent in healthy and fibrosis smokers ( 2.5-fold increase in p50 subunit translocation )
Hattori et al., Hypertension 2006 : Metformin also dose-dependently inhibited tumor necrosis factor (TNF)-alpha induced NF-kappaB activation and TNF-alpha induced IkappaB kinase activity ... A pharmacological activator of AMPK , 5-amino-4-imidazole carboxamide riboside ( AICAR ), dose-dependently inhibited TNF-alpha- and interleukin-1beta induced NF-kappaB reporter gene expression ... A pharmacological activator of AMPK, 5-amino-4-imidazole carboxamide riboside ( AICAR ), dose-dependently inhibited TNF-alpha- and interleukin-1beta induced NF-kappaB reporter gene expression ... In light of these findings, we suggest that metformin attenuates the cytokine induced expression of proinflammatory and adhesion molecule genes by inhibiting NF-kappaB activation via AMPK activation
Davis et al., Experimental biology and medicine (Maywood, N.J.) 2006 : AE after a 24-hr incubation period at either 200 or 2000 nM showed a complex pattern of decreased basal and TNF-alpha stimulated NF-kappaB signaling ( 63 % maximal decrease ) as assessed by luciferase activity in the transfected HUVECs, as well as by reduced levels of IkappaBalpha protein phosphorylation detected by Western blot analysis
Chang et al., Nephron. Experimental nephrology 2006 (Inflammation) : NF-kappaB was activated within 30 min by tumor necrosis factor-alpha (TNF-alpha) or interleukin-1beta (IL-1beta) ... NF-kappaB was activated within 30 min by tumor necrosis factor-alpha (TNF-alpha) or interleukin-1beta (IL-1beta) ... Intracellular ROS was not produced until 30 min and also antioxidants such as N-acetylcysteine and tiron had no effect on the TNF-alpha- or IL-1beta induced NF-kappaB activation ... Silymarin dose-dependently inhibited the TNF-alpha- or IL-1beta induced NF-kappaB activation and MCP-1 expression ... Induction of NF-kappaB within 30 min by TNF-alpha- and IL-1beta was mediated through intracellular calcium but not ROS ... Induction of NF-kappaB within 30 min by TNF-alpha- and IL-1beta was mediated through intracellular calcium but not ROS ... Silymarin inhibited TNF-alpha induced calcium dependent NF-kappaB activation irrespective of its antioxidant effect
Choi et al., Mol Carcinog 2006 (Colonic Neoplasms) : These results taken together suggest that the inhibition of IkappaB and activation of NFkappaB activate SSAT
Liu et al., J Agric Food Chem 2006 : DATS reduces LPS induced iNOS expression, NO production, oxidative stress, and NF-kappaB activation in RAW 264.7 macrophages ... LPS induced cellular peroxide production was significantly inhibited by DADS and DATS ( P < 0.05 ) but not by DAS. Electrophoresis mobility shift assays further indicated that DADS and DATS effectively inhibited the activation of NF-kappaB induced by LPS. Taken together, these results indicate that the differential efficacy of three major OSCs of garlic oil on suppression of iNOS expression and NO production is related to the number of sulfur atoms and is in the order DATS > DADS > DAS
Minoda et al., Biochem Biophys Res Commun 2006 : Overexpression and knockdown by siRNA indicated that ZCCHC11 functions as a negative regulator of TLR mediated NF-kappaB activation ... We propose that ZCCHC11 is a unique TLR signal regulator, which interacts with TIFA after LPS treatment and suppresses the TRAF6 dependent activation of NF-kappaB
Choi et al., Int Immunopharmacol 2006 : A few previous studies reported that inhibition of the phosphatidylinositol 3-kinase (PI3K)-Akt pathway enhances lipopolysaccharide (LPS) induced activation of NF-kappaB ... However, many aspects of the PI3K negative regulation of NF-kappaB activation remain to be clarified ... The present study was conducted to shed light on cell-type specificity, stimulus specificity, and upstream mechanisms of the enhanced NF-kappaB activation by PI3K inhibitors ... Gel shift assays showed that LY294002 ( LY29 ) potently increased interleukin (IL)-1 induced NF-kappaB DNA binding in human monocytic THP-1 cells ... Moreover, another PI3K inhibitor 3-methyladenine also strongly enhanced IL-1 induced NF-kappaB DNA binding, while LY303511, an inactive analogue of LY29, did not increase the NF-kappaB DNA binding ... LY29 treatment also augmented tumor necrosis factor (TNF) mediated NF-kappaB DNA binding
Shin et al., Int Immunopharmacol 2006 : Since nuclear factor (NF)-kappaB activation has been shown to play a key role in LPS induced IL-6 expression, the effect of NFD-37 on LPS induced NF-kappaB activation was further analyzed ... NFD-37 exhibited a dose dependent inhibitory effect on LPS induced phosphorylation of inhibitory kappaB alpha protein ( IkappaB alpha ), and subsequently inhibited LPS induced IkappaB alpha degradation as well as NF-kappaB transcriptional activity
Starkey et al., Diabetes 2006 (Diabetes Mellitus, Experimental) : Cytosolic content of NF-kappaB canonical pathway proteins did not differ between experimental groups after 3 months of diabetes, while NF-kappaB noncanonical pathway proteins were affected, including increased phosphorylation of inhibitor of kappaB kinase-alpha and several fold increases in NF-kappaB inducing kinase and RelB , which were predominantly located in tubular epithelial cells
Fitau et al., J Biol Chem 2006 : Mechanistically, our data indicated that, in response to TNFalpha , NFkappaB/p65 phosphorylation and translocation as well as IkappaBalpha phosphorylation and degradation were unchanged, suggesting that Lnk does not modulate NFkappaB activity
Guillermet-Guibert et al., Cell Death Differ 2007 : Tumor necrosis factor alpha (TNFalpha) stimulated both NF-kappaB and c-Jun NH2-terminal kinase (JNK) activities, which had opposite action on cell survival ... Importantly, sst2 sensitized NIH3T3 cells to TNFalpha induced apoptosis by ( 1 ) upregulating TNFalpha receptor protein expression, and sensitizing to TNFalpha induced caspase-8 activation ; ( 2 ) enhancing TNFalpha mediated activation of NF-kappaB , resulting in JNK inhibition and subsequent executioner caspase activation and cell death
Williams et al., Cell Death Differ 2007 (Adenoma...) : Both addition of IGFBP-3 protein to cell cultures or enforced expression of IGFBP-3 in the HT29 carcinoma cell line inhibited nuclear factor kappa B (NF-kappaB) activation in response to the induction of apoptosis by TRAIL
Li et al., J Cell Physiol 2006 : Expression of A20 has been shown to protect from TNF induced apoptosis and also functions via a negative-feedback loop to block NF-kappaB activation induced by TNF and other stimuli
Kim et al., Biochem Biophys Res Commun 2006 : Inhibition of NF-kappaB activation resulted in diminished CO-induced cFLIP expression and increased caspase-8 cleavage from cells treated with TNFalpha/ActD
Chan et al., Cytokine 2006 (Cell Transformation, Viral) : In human bronchial epithelial cells, binding sites for NFkappaB , AP-1, and NF-IL6 in the 5'-flanking region of the IL-8 promoter are necessary for optimal NaCl induction of IL-8
Rivera et al., Trends Immunol 2006 : The CARMA1-Bcl10-Malt1 adaptor complex regulates NFkappaB activation by antigen receptors in lymphocytes
Nelson et al., Curr Opin Genet Dev 2006 (Disease Models, Animal...) : Recent advances in mouse models and in understanding the multiple roles of NEMO in the cell provide additional avenues to define the various roles of NEMO in NFkappaB signaling
Franchi et al., Nat Immunol 2006 (Salmonella Infections) : Here we demonstrate that salmonella strains either lacking flagellin or expressing mutant flagellin were deficient in activation of caspase-1 and in interleukin 1beta secretion, although transcription factor NF-kappaB dependent production of interleukin 6 or the chemokine MCP-1 was unimpaired ... Here we demonstrate that salmonella strains either lacking flagellin or expressing mutant flagellin were deficient in activation of caspase-1 and in interleukin 1beta secretion, although transcription factor NF-kappaB dependent production of interleukin 6 or the chemokine MCP-1 was unimpaired ... Here we demonstrate that salmonella strains either lacking flagellin or expressing mutant flagellin were deficient in activation of caspase-1 and in interleukin 1beta secretion, although transcription factor NF-kappaB dependent production of interleukin 6 or the chemokine MCP-1 was unimpaired
Heon Seo et al., Cancer Res 2006 (Melanoma, Experimental) : These data indicate that PAF induces up-regulation of antiapoptotic factors in a NF-kappaB dependent manner in a melanoma cell line, therefore suggesting that PAF may diminish the cytotoxic effect of chemotherapeutic agents
Park et al., Biol Pharm Bull 2006 : Furthermore, GBB inhibited the LPS induced DNA binding activity of nuclear factor-kappaB (NF-kappaB) , which was associated with the prevention of IkappaB degradation, and subsequently with decreased p65 protein level in the nucleus
Charrier et al., Lab Invest 2006 (Inflammation...) : The small bacterial peptides subsequently induce an inflammatory response, including the induction of MHC class I molecules expression and cytokines secretion, via the activation of nucleotide binding site and leucine-rich repeat ( NBS-LRR ) proteins, for example NOD2 , and activation of NF-kappaB
Grant et al., PLoS Pathog 2006 : Overexpression of Foxp3 in HEK 293T and purified CD4+ T cells resulted in a dose dependent and time dependent decrease in basal levels of nuclear factor-kappaB (NF-kappaB) activation ... These results suggest an expanded role for Foxp3 in regulating NF-kappaB- and CREB dependent cellular and viral gene expression
Kuo et al., J Immunol 2006 : Our results reveal that class III PI3K specifically regulates CpG oligodeoxynucleotide ( ODN ) -induced cytokine and NO production as well as NF-kappaB activation, whereas class I PI3K regulates both CpG ODN- and LPS induced IL-12 production and NF-kappaB activation ... Our results reveal that class III PI3K specifically regulates CpG oligodeoxynucleotide ( ODN ) -induced cytokine and NO production as well as NF-kappaB activation, whereas class I PI3K regulates both CpG ODN- and LPS induced IL-12 production and NF-kappaB activation
Kim et al., J Immunol 2006 : Adiponectin was found to suppress the IL-2 enhanced cytotoxic activity of NK cells without affecting basal NK cell cytotoxicity and to inhibit IL-2 induced NF-kappaB activation via activation of the AMP activated protein kinase, indicating that it suppresses IL-2 enhanced NK cell cytotoxicity through the AMP activated protein kinase mediated inhibition of NF-kappaB activation
Fujigaki et al., J Biochem 2006 : Further, the LPS induced IDO activity was inhibited by both p38 mitogen activated protein kinase ( MAPK ) and nuclear factor-kappaB (NF-kappaB) inhibitors
Brahmachari et al., J Neurosci 2006 : Interestingly, inhibition of nuclear factor kappaB (NF-kappaB) suppressed LPS- but not GSNO induced expression of GFAP, suggesting that NO does not require NF-kappaB to induce GFAP and that NF-kappaB functions upstream of NO production ... Interestingly, inhibition of nuclear factor kappaB (NF-kappaB) suppressed LPS- but not GSNO induced expression of GFAP, suggesting that NO does not require NF-kappaB to induce GFAP and that NF-kappaB functions upstream of NO production
Li et al., Inflamm Res 2006 : To investigate the effect of verapamil on Lipopolysaccharide (LPS) induced cytokines [ tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-10 (IL-10) ] and nuclear factor kappa B (NF-kappa B) in the liver ... LPS alone stimulated production of TNF-alpha, IL-6 and IL-10, and activated NF-kappa B in the liver
Wolf et al., J Am Soc Nephrol 2006 (Inflammation) : Preincubation of MMC with AngII enhanced LPS induced NF-kappaB activation and chemokine expression
Levy et al., Am J Physiol Regul Integr Comp Physiol 2006 (Femoral Fractures...) : Trauma induced nuclear factor (NF)-kappaB activation in the liver required functional TLR4 signaling
Debret et al., J Invest Dermatol 2006 (MAP Kinase Signaling System...) : Concomitantly, we demonstrated that EFs stimulation induced NF-kappaB nuclear translocation and DNA binding on IL-1beta promoter region whereas inhibition of NF-kappaB with the specific chemical inhibitor SN-50 or by overexpression of IkappaB, the endogenous inhibitor of NF-kappaB pathway, totally abolished EFs mediated IL-1beta mRNA overexpression ... Concomitantly, we demonstrated that EFs stimulation induced NF-kappaB nuclear translocation and DNA binding on IL-1beta promoter region whereas inhibition of NF-kappaB with the specific chemical inhibitor SN-50 or by overexpression of IkappaB, the endogenous inhibitor of NF-kappaB pathway, totally abolished EFs mediated IL-1beta mRNA overexpression ... These results demonstrate that EFs induce NF-kappaB activation, leading to IL-1beta upregulation in invasive melanoma cells
Youn et al., Biochem Pharmacol 2006 : LPS induces the activation of NF-kappaB and IRF3 through MyD88- or TRIF dependent pathways
Kooncumchoo et al., J Mol Neurosci 2006 (Parkinson Disease) : In this study we have determined the neuroprotective role of coenzyme Q ( 10 ) ( CoQ ( 10 ) ) in ironinduced apoptosis in cultured human dopaminergic ( SK-N-SH ) neurons, in metallothionein gene- manipulated mice, and in alpha-synuclein knockout ( alpha-synko ) mice with a primary objective to assess a possible therapeutic and anti-inflammatory potential for CoQ ( 10 ) in PD. Iron induced mitochondrial damage and apoptosis were characterized by reactive oxygen species production, increased metallothionein and glutathione synthesis, caspase- 3 activation, NF-kappaB induction , and decreased Bcl-2 expression, without any significant change in Bax expression
Welter-Stahl et al., Cell Microbiol 2006 (Chlamydia Infections) : We find that overexpression of a dominant negative Nod1 or depletion of Nod1 by RNA interference inhibits partially the activation of NF-kappaB during chlamydial infection in vitro, suggesting that Nod1 can detect the presence of Chlamydia
Ahn et al., J Biol Chem 2006 : TNF induced nuclear factor (NF)-kappaB activation in wild-type but not in NQO1 deleted cells ... The treatment of wild-type cells with dicoumarol, a known inhibitor of NQO1, also abolished TNF induced NF-kappaB activation ... We also found that TNF activated NQO1, and NQO1-specific small interfering RNA abolished the TNF induced NQO1 activity and NF-kappaB activation
Golks et al., J Exp Med 2006 (Neoplasms) : Here, we demonstrate that the cytoplasmic NH2-terminal procaspase-8 cleavage product of c-FLIP ( p22-FLIP ) found in nonapoptotic malignant cells, primary T and B cells, and mature dendritic cells (DCs) strongly induces nuclear factor kappaB (NF-kappaB) activity by interacting with the IkappaB kinase (IKK) complex via the IKKgamma subunit ... Thus, in addition to inhibiting apoptosis by binding to the death inducing signaling complex, our data demonstrate a novel mechanism by which c-FLIP controls NF-kappaB activation and life/death decisions in lymphocytes and DCs
Armstrong et al., Prostate 2006 (Prostatic Neoplasms) : NF-kappaB activation upregulates fibroblast growth factor 8 expression in prostate cancer cells ... Using TSA treatment in prostate cancer cells, a requirement of PI-3K activity in mediating TSA function is demonstrated and a novel role for NF-kappaB in the regulation of FGF8 expression is uncovered
Lu et al., Exp Eye Res 2006 (Corneal Ulcer) : IL-1 induced the phosphorylation and degradation of IkappaB-alpha, the nuclear translocation and up-regulation of the DNA binding activity of the p65 subunit of NF-kappaB, and the activation of NF-kappaB in a manner sensitive to sulfasalazine ... IL-1 induced the phosphorylation and degradation of IkappaB-alpha , the nuclear translocation and up-regulation of the DNA binding activity of the p65 subunit of NF-kappaB, and the activation of NF-kappaB in a manner sensitive to sulfasalazine
Batmunkh et al., FEBS Lett 2006 : Western blot analyses and electrophoretic mobility shift assays ( EMSAs ) revealed that IL-1 strongly activated NF-kappaB , which is a likely suppressor of the Epo promoter
Ko et al., FEBS Lett 2006 : Platelet activating factor induced NF-kappaB activation enhances VEGF expression through a decrease in p53 activity ... We investigated the role of p53 in nuclear factor (NF)-kappaB dependent , platelet activating factor (PAF) induced vascular endothelial growth factor ( VEGF ) expression ... These data suggest that NF-kappaB-dependency of the PAF induced increase in VEGF expression is due to decreased p53 activity, which is reciprocally regulated by increased NF-kappaB activity
Mauro et al., J Biol Chem 2006 : Canonical activation of NF-kappaB is dependent on the phosphorylation of the inhibitory subunit IkappaBalpha that is mediated by a multimeric, high molecular weight complex, called IkappaB kinase (IKK) complex ... We find that both ABIN-1 and A20 inhibit NF-kappaB at the level of the IKK complex and that A20 inhibits activation of NF-kappaB by de-ubiquitination of NEMO/IKKgamma
Henness et al., Am J Physiol Lung Cell Mol Physiol 2006 (Lung Neoplasms) : As IL-17A had no effect on the activity of NF-kappaB , a key transcriptional regulator of IL-8 gene expression, we then examined whether IL-17A acts at the posttranscriptional level ... As IL-17A had no effect on the activity of NF-kappaB , a key transcriptional regulator of IL-8 gene expression, we then examined whether IL-17A acts at the posttranscriptional level
Piazza et al., Blood 2006 (Multiple Myeloma) : CK2 inhibition also resulted in impaired IL-6 dependent STAT3 activation and in decreased basal and TNF-alpha dependent I kappaB alpha degradation and NF-kappaB-driven transcription
Villalona-Calero et al., Cancer Chemother Pharmacol 2007 (Neoplasms) : Preclinically, thalidomide and COX-2 inhibitors reduce NF-kappaB activation
Wang et al., Chin J Cancer 2006 (Neoplasm Invasiveness...) : HGF promoted the expression of NF-kappaB protein in a time dependent manner
Zhao et al., J Biol Chem 2006 : We have demonstrated earlier that lysophosphatidic acid (LPA) induced interleukin-8 (IL-8) secretion is regulated by protein kinase Cdelta (PKCdelta) dependent NF-kappaB activation in human bronchial epithelial cells ( HBEpCs ) ... We have demonstrated earlier that lysophosphatidic acid (LPA) induced interleukin-8 (IL-8) secretion is regulated by protein kinase Cdelta (PKCdelta) dependent NF-kappaB activation in human bronchial epithelial cells ( HBEpCs )
Tang et al., J Am Soc Nephrol 2006 (Diabetic Nephropathies...) : Also in a dose dependent manner, GA ( 0.5 mg/ml ) but not albumin caused nuclear translocation of NF-kappaB and activation of mitogen activated protein kinase ( MAPK ) p44/p42 and signal transducer and activator of transcription ( STAT-1 ) ... Also in a dose dependent manner, GA ( 0.5 mg/ml ) but not albumin caused nuclear translocation of NF-kappaB and activation of mitogen activated protein kinase ( MAPK ) p44/p42 and signal transducer and activator of transcription ( STAT-1 )
Yuan et al., World J Gastroenterol 2006 (Liver Cirrhosis, Alcoholic) : The enhanced iNOS activity and expression were positively correlated with the liver damage, especially the necro-inflammation, activation of NF-kappaB , and TNF-alpha mRNA expression
Jatana et al., Journal of neuroinflammation 2006 : BW-B 70C down-regulated 5-LOX and inhibited iNOS expression by preventing NF-kappaB activation ... BW-B 70C down-regulated 5-LOX and inhibited iNOS expression by preventing NF-kappaB activation ... Furthermore, in vitro, BW-B 70C inhibited lipopolysaccharide (LPS) mediated nitric oxide production, iNOS induction and NF-kappaB activation in the BV2 microglial cell line ... Furthermore, in vitro, BW-B 70C inhibited lipopolysaccharide (LPS) mediated nitric oxide production, iNOS induction and NF-kappaB activation in the BV2 microglial cell line ... Furthermore, in vitro, BW-B 70C inhibited lipopolysaccharide (LPS) mediated nitric oxide production, iNOS induction and NF-kappaB activation in the BV2 microglial cell line
Rangaswami et al., Glycoconj J 2006 (Melanoma) : OPN induces NIK dependent NF-kappaB activation through ERK/IKKalpha/beta mediated pathways ... Taken together, NIK acts as crucial regulator in OPN induced MAPK/IKK mediated NF-kappaB dependent uPA secretion and MMP-9 activation thereby controlling melanoma cell motility and chemoinvasion ... Taken together, NIK acts as crucial regulator in OPN induced MAPK/IKK mediated NF-kappaB dependent uPA secretion and MMP-9 activation thereby controlling melanoma cell motility and chemoinvasion
Chen et al., FEBS Lett 2006 : Hsp70 inhibits lipopolysaccharide induced NF-kappaB activation by interacting with TRAF6 and inhibiting its ubiquitination ... Here we report a novel mechanism of Hsp70 for preventing LPS induced NF-kappaB activation in RAW264.7 macrophage-like cells ... Taken together, these findings indicate that Hsp70 inhibits LPS induced NF-kappaB activation by binding TRAF6 and preventing its ubiquitination, and results in inhibition of inflammatory mediator production, which provides a new insight for analyzing the effects of Hsp70 on LPS triggered inflammatory signal transduction pathways
Ding et al., J Mol Cell Cardiol 2006 (Inflammation) : Moreover, activation of PPARdelta abrogated LPS induced degradation of IkappaBs, thus suppressing LPS induced nuclear factor kappaB (NF-kappaB) activities
Molinero et al., Hum Immunol 2006 : IL-2 effect involved Jak3/STAT5, p38 MAPK, p70 ( 56 ) kinase, Lck/fyn kinases, and NF-kappaB
Rahman et al., J Lipid Res 2006 (Bone Resorption) : Treatment of these cells with RANKL activated nuclear factor-kappaB (NF-kappaB), and preexposure of the cells to CLA significantly suppressed RANKL induced NF-kappaB activation, including phosphorylation of I-kappaBalpha, degradation of I-kappaBalpha, and nuclear translocation of p65
Strozyk et al., Oncogene 2006 (Chromosome Breakage) : In contrast, ultraviolet light B ( UVB ) -induced apoptosis is not inhibited but even enhanced upon NF-kappaB activation by interleukin-1 (IL-1) ... Surprisingly, activated NF-kappaB induced TNF-alpha mRNA expression in the presence of all DNA damage inducing agents
Chen et al., Oncogene 2006 (Multiple Myeloma) : We found that siRNA targeting the TRAF6 C-terminal ( siTRAF6C ) receptor interaction domain specifically reduced only TRAF6 protein expression, without affecting TRAF2 or 5 levels, and substantially interfered with IL-1 induced NF-kappaB and c-Jun/AP-1 activation
Tsuchiya et al., Am J Physiol Endocrinol Metab 2006 (Obesity) : ANG II induced translocation of NF-kappaB p65 subunit from cytoplasm to nucleus by immunocytochemical study
Hayakawa et al., American journal of physiology. Renal physiology 2006 (MAP Kinase Signaling System) : Selective suppression of ERK or p38 MAP kinase significantly attenuated activation of NF-kappaB in mesangial cells triggered by coculture with isolated glomeruli
Yeow et al., Br J Cancer 2006 (Neoplasms) : The weak VA-mediated induction of apoptosis of thoracic cancer cells can be profoundly enhanced either by Parthenolide, a pharmacologic inhibitor of NF-kappaB , or by UCN-01 a kinase inhibitor that has already undergone phase I clinical development
Schiekofer et al., Exp Clin Endocrinol Diabetes 2006 (Diabetes Complications...) : Postprandial mononuclear NF-kappaB activation is independent of the AGE-content of a single meal ... Postprandial mononuclear NF-kappaB activation after a single meal is independent of the AGE-content of the ingested protein
Kafoury et al., International journal of environmental research and public health 2005 : DEP induced TNF-alpha gene expression is regulated at the transcriptional level by NF-kappaB
Jeong et al., Biochem Biophys Res Commun 2006 : Although H ( 2 ) S by itself showed no effect on NO production, it augmented IL-beta induced NO production and this effect was associated with increased expression of inducible NO synthase (iNOS) and activation of nuclear factor (NF)-kappaB ... IL-1Beta activated the extracellular signal regulated kinase 1/2 ( ERK1/2 ), and this activation was also enhanced by H ( 2 ) S. Inhibition of ERK1/2 activation by the selective inhibitor U0126 inhibited IL-1beta induced NF-kappaB activation, iNOS expression, and NO production either in the absence or presence of H ( 2 ) S ... Our findings suggest that H ( 2 ) S enhances NO production and iNOS expression by potentiating IL-1beta induced NF-kappaB activation through a mechanism involving ERK1/2 signaling cascade in rat VSMCs
Ren et al., Cancer Res 2006 (Carcinoma, Squamous Cell...) : NF-kappaB activation was largely provided through interaction with extracellular matrix components because preventing cell attachment by forced suspension culture markedly reduced NFkappaB signaling associated with cell death ( anoikis ) ; conversely, anoikis was partially reversed by NF-kappaB activation induced either by tumor necrosis factor-alpha treatment or by overexpressing the NF-kappaB p65 subunit in HaCaT cells
Imre et al., Cancer Res 2006 (Carcinoma, Non-Small-Cell Lung...) : HDI treated A549 and NCI-H460 cells down-regulated TNF-alpha receptor-1 mRNA and protein levels as well as surface exposure, and consequently responded to TNF-alpha treatment with reduced IKK phosphorylation and activation, delayed IkappaB-alpha phosphorylation, and attenuated NF-kappaB nuclear translocation and DNA binding ... Accordingly, stimulation of NF-kappaB target gene expression by TNF-alpha was strongly decreased
Yamagishi et al., J Biol Chem 2006 (Diabetic Retinopathy) : This led to blockade of the AGE elicited Ras activation and NF-kappaB dependent VEGF gene induction in endothelial cells
Ghosh-Choudhury et al., J Biol Chem 2006 : BMP-2 stimulates receptor activator of NF-kappaB ligand expression
Li et al., J Am Soc Nephrol 2006 : It was reported recently that interaction between ouabain bound Na, K-ATPase and the 1,4,5-trisphosphate receptor ( IP3R ) triggers slow calcium oscillations and activation of NF-kappaB
Paria et al., J Biol Chem 2006 : Inhibition of store Ca ( 2+ ) depletion, buffering of intracellular Ca ( 2+ ), or down-regulation of protein kinase Calpha downstream of Ca ( 2+ ) influx all blocked thrombin induced NF-kappaB activation and the resultant TRPC1 expression in endothelial cells
Chen et al., Arterioscler Thromb Vasc Biol 2006 : The balance between caspase-8 and FLIP favored proliferation in SM22-PAI+-VSMCs. Increased expression of NF-kappaB and activation of extracellular signal regulated kinase ( ERK ) were demonstrated in SM22-PAI+-VSMCs ( fold=NF-kappaB=2.2+/-0.1, fold=phosphorylated-ERK=1.6+/-0.1 )
Massoumi et al., Cell 2006 (Skin Neoplasms) : Cyld inhibits tumor cell proliferation by blocking Bcl-3 dependent NF-kappaB signaling
Sun et al., Immunity 2006 (Listeriosis...) : By generating MAVS-deficient mice, here we show that loss of MAVS abolished viral induction of interferons and prevented the activation of NFkappaB and IRF3 in multiple cell types, except plasmacytoid dendritic cells ( pDCs )
Mormina et al., Eur J Pharmacol 2006 : TNF-alpha induced release of IL-8 was inhibited by WIN55212-2 which also blocked the degradation of IkappaB-alpha and activation of NF-kappaB induced by TNF-alpha ... TNF-alpha induced release of IL-8 was inhibited by WIN55212-2 which also blocked the degradation of IkappaB-alpha and activation of NF-kappaB induced by TNF-alpha
Faour et al., J Biol Chem 2006 (Arthritis, Rheumatoid...) : Role in suppression of c/EBP/NF-kappaB mediated MEKK1 induced MMP-1 expression
Vogel et al., Am J Physiol Lung Cell Mol Physiol 2006 : TNF-alpha caused a progressive increase in NF-kappaB activity after 0.5 h and ICAM-1 protein expression two- to threefold of basal after 2 h. Untreated lungs expressed a low and constant level of ICAM-1 between 0 and 3.5 h. TNF-alpha failed to induce NF-kappaB activation and ICAM-1 expression in lungs of NADPH oxidase-deficient mice lacking p47(phox)
Kufer et al., Infect Immun 2006 : Furthermore, ectopic expression of increasing amounts of Erbin or short hairpin RNA mediated knockdown of Erbin showed a negative influence of Erbin on Nod2/muramyl-dipeptide mediated NF-kappaB activation
Parsons et al., Oncogene 2006 (Cell Transformation, Neoplastic) : We further show that whereas TMS1/ASC is not required for TNFalpha or TRAIL induced activation of NF-kappaB or caspase-8, it can promote caspase-8 activation independently of death receptor-ligand interactions
Eisner et al., FEBS Lett 2006 : Supershift analysis revealed that p65 and RelB ( but not p50, p52 or cRel ) were involved in the binding of NFkappaB to DNA ... Supershift analysis revealed that p65 and RelB ( but not p50, p52 or cRel ) were involved in the binding of NFkappaB to DNA ... Supershift analysis revealed that p65 and RelB ( but not p50, p52 or cRel ) were involved in the binding of NFkappaB to DNA ... We conclude that hyperosmotic stress activates p65 and RelB NFkappaB isoforms and NFkappaB mediates caspase 9 activation in cardiomyocytes
Woo et al., Immunobiology 2006 : Baicalein and baicalin had no effect on LPS induced nuclear factor-kappaB (NF-kappaB) and cAMP response element binding protein ( CREB ) DNA binding activity
Nguyen et al., Immunol Lett 2006 : In this study, we investigated the intracellular signaling pathways underlying TNF-alpha/NF-kappaB dependent expression of MMP-9 in monocytes using chemical inhibitors that specifically inhibit distinct kinase pathways
Lee et al., Frontiers in bioscience : a journal and virtual library 2006 (Inflammation...) : Previously we showed that the overexpression of transglutaminase 2 (TGase 2) resulted in activation of NF-kappaB through polymerization of I-kappaBalpha
Zhu et al., Br J Cancer 2006 (Anoxia...) : We demonstrate that expression of GCP-2 is mediated by NF-kappaB as ALLN, an NF-kappaB pathway inhibitor, almost completely abolished GCP-2 production in SCLC cell lines
Lee et al., Int J Cancer 2006 (MAP Kinase Signaling System) : DNA binding activity of nuclear factor (NF)-kappaB , but not that of activator protein (AP)-1, was increased by H-Ras
Thommesen et al., J Cell Biochem 2006 : Recombinant resistin increased the number of differentiated osteoclasts and stimulated NFkappaB promoter activity, indicating a role in osteoclastogenesis
Kao et al., Exp Gerontol 2006 (Arthritis, Rheumatoid) : The NF-kappaB inhibitor pyrrolidine dithiocarbamate blocks IL-1beta induced hyaluronan synthase 1 (HAS1) mRNA transcription, pointing at NF-kappaB dependence of the gene HAS1 ... EMSA data confirm that PDCT, at concentrations sufficient to completely block IL-1beta induced HAS1 transcription, also entirely blocks IL-1beta induced NF-kappaB translocation
Sakoda et al., J Dent Res 2006 : Dominant negative Rac1 severely inhibited interleukin-1alpha induced NF-kappaB and AP-1 promoter activity ... Dominant negative Rac1 severely inhibited interleukin-1alpha induced NF-kappaB and AP-1 promoter activity
Islam et al., Cancer Chemother Pharmacol 2007 : Therefore, 5-FU was suggested to inhibit the LPS induced NO production in activated macrophages through preventing Akt dependent NF-kappaB activation
Lin et al., Cell Signal 2006 : The results demonstrate a novel mechanism for transcriptional regulation of E2F1 and identify the functional role of nucleophosmin/B23 in modulating the binding of NF-kappaB , E2F1 and pRB to activate E2F1 promoter
Okabe et al., J Endod 2006 : These results suggest that NF- kappaB and Smad7 play an important role in the down regulation of ALP activity by TNF-alpha on HDP cells
Mohammad et al., Int Immunol 2006 (Diabetes Mellitus, Experimental) : Dysregulation of TLR4 expression in the diabetic state correlated with increased nuclear factor kappa B (NF-kappaB) activation in response to the TLR4 ligand LPS and higher expression of IL-12p40, tumor necrosis factor alpha (TNFalpha), IL-6 and inducible nitric oxide synthase but lowered expression of IL-10
Harada et al., Diabetes Res Clin Pract 2006 (Diabetic Retinopathy...) : In vitro studies demonstrated that glycated albumin or high glucose induces NF-kappaB activation followed by up-regulation of MCP-1 promoter activity and protein production in glial cells
Singh et al., Mol Cancer Ther 2006 (MAP Kinase Signaling System) : Silibinin treatment under similar conditions also strongly inhibited EGF induced ERK1/2, JNK1/2, and p38K as well as Akt phosphorylation, and also suppressed EGF induced AP-1 and NF-kappaB activation
Menini et al., Diabetes 2006 (Albuminuria...) : Serum and renal tissue advanced glycation end products and plasma isoprostane 8-epi-prostaglandin F2alpha levels and activation of nuclear factor kappaB (NF-kappaB) were also lower in diabetic KO than in wild-type mice
Aktan et al., Planta Med 2006 : Taken together, these results show that ZTX42 and [ 6 ] -DHP suppress NO production in murine macrophages by partially inhibiting iNOS enzymatic activity and reducing iNOS protein production, via attenuation of NF-kappaB mediated iNOS gene expression, providing a rationale for the anti-inflammatory activity reported for this class of compounds
Yamanaka et al., J Orthop Res 2006 (Bone Resorption) : We have also found that polymethyl methacrylate ( PMMA ) particles stimulate osteoclastogenesis, at least in part, by induction of RANKL , TNF, and by activation of NF-kappaB and MAP kinases
Zheng et al., Hum Pathol 2006 (Lupus Nephritis) : The mechanism of renal cell apoptosis involves transcriptional activation of the inducible nitric oxide synthase (iNOS) gene by nuclear factor (NF)-kappaB
Hosokawa et al., Clin Exp Immunol 2006 : Both ICAM-1 and VCAM-1 expression by HGF was inhibited by nuclear factor-kappaB (NF-kappaB) activation inhibitor ( MG-132 ) ... Both ICAM-1 and VCAM-1 expression by HGF was inhibited by nuclear factor-kappaB (NF-kappaB) activation inhibitor ( MG-132 )
Dolcet et al., J Biol Chem 2006 (Endometrial Neoplasms) : Proteasome inhibitor induced NF-kappaB activity can be blocked by a non-degradable form of IkappaB alpha or dominant negative forms of either IKK alpha or IKK beta ... Proteasome inhibitor induced NF-kappaB activity can be blocked by a non-degradable form of IkappaB alpha or dominant negative forms of either IKK alpha or IKK beta ... These results suggest a role for p65 phosphorylation in proteasome inhibitor induced NF-kappaB activation
Kearns et al., J Cell Biol 2006 (Inflammation) : Upon stimulation, NF-kappaB induced transcription of IkappaBepsilon is delayed, relative to that of IkappaBalpha, rendering the two negative feedback loops to be in antiphase
Lee et al., Arthritis Rheum 2006 (Arthritis, Rheumatoid) : CD40 ligation of RASFs also induced activation of ERK-1/2, p38 MAPK, and NF-kappaB and up-regulation of CD40 ligation induced RANKL expression, whereas osteoclastogenesis was reduced in RASFs transfected with a dominant negative mutant of IkappaB alpha or by an NF-kappaB inhibitor
Qin et al., J Biol Chem 2006 : TLR8 mediated NF-kappaB and JNK activation are TAK1 independent and MEKK3 dependent ... TLR8 ligands trigger similar levels of IkappaBalpha phosphorylation and NF-kappaB and JNK activation in TAK1 ( -/- ) mouse embryo fibroblasts ( MEFs ) as compared with wild-type MEFs, whereas lack of TAK1 results in reduced IL-1 mediated NF-kappaB activation and abolished IL-1 induced JNK activation ... The above results indicate that although TLR8 mediated NF-kappaB and JNK activation are IRAK dependent , they do not require IRAK modification and are TAK1 independent ... On the other hand, TLR8 mediated IkappaBalpha phosphorylation, NF-kappaB , and JNK activation are completely abolished in MEKK3 ( -/- ) MEFs, whereas IL-1 mediated signaling was only moderately reduced in these deficient MEFs as compared with wild-type cells
Martin-Oliva et al., Cancer Res 2006 (Cell Transformation, Neoplastic...) : Inhibition of PARP also affected activator protein-1 (AP-1) activation but not nuclear factor-kappaB (NF-kappaB)
Takahashi et al., Clin Cancer Res 2006 (Chromosome Aberrations...) : Herein, we ask whether FAS death domain mutations and NFkappaB mediated overexpression of cFLIP represent alternative mechanisms for deregulating the extrinsic apoptotic pathway in LBCL subtypes defined by gene expression profiling [ oxidative phosphorylation, B-cell receptor/proliferation, and host response diffuse LBCLs and primary mediastinal LBCLs ]
López-Franco et al., Arterioscler Thromb Vasc Biol 2006 (Atherosclerosis...) : In this work we analyzed the effects of the natural compound parthenolide (PTN) , an NF-kappaB inhibitor
Lewis et al., J Invest Dermatol 2006 : We demonstrate that NF-kappaB DNA binding is activated by both UVB and TNFalpha , but discrepancies in the activation of key upstream signaling pathway components such as AKT phosphorylation and IkappaBalpha degradation exist
Lok et al., J Lab Clin Med 2006 (Pre-Eclampsia) : IL-1alpha enhanced the expression of IL-1alpha, IL-2, IL-6, and IL-8 ; nuclear factor of kappa light chain enhancer in B-cells ( NFkappaB ) -1, NFkappaB-2, and NFkappaB-inhibitor ; cyclin dependent kinase inhibitor and monocyte chemotactic protein-1 ; and transiently increased tissue factor expression
Gobejishvili et al., Am J Physiol Gastrointest Liver Physiol 2006 (Liver Diseases, Alcoholic) : Consistent with the decrease in cAMP levels, ethanol led to an increase in LPS-inducible TNF-alpha production by affecting NF-kappaB activation and induction of TNF mRNA expression, without any change in TNF mRNA stability
Fabisiak et al., Am J Physiol Lung Cell Mol Physiol 2006 (Lung Diseases...) : Exposure to M. fermentans or MALP-2 did not enhance nuclear localization, DNA binding, or transcriptional activity of NF-kappaB and did not modulate early NF-kappaB activation in response to TNF-beta
Medoff et al., J Immunol 2006 (Asthma...) : CARMA1 has been shown to be important for Ag-stimulated activation of NF-kappaB in lymphocytes in vitro and thus could be a novel therapeutic target in inflammatory diseases such as asthma
Nguyen et al., J Immunol 2006 : BTNL2-Ig inhibited T cell proliferation and TCR activation of NFAT, NF-kappaB , and AP-1 signaling pathways
Zhang et al., J Immunol 2006 (Arthritis, Rheumatoid...) : The RASF derived exosomes, but not exosomes derived from fibroblasts obtained from individuals with osteoarthritis, are cytotoxic for the L929 cell, a TNF-alpha-sensitive cell line, and stimulate activation of NF-kappaB and induction of collagenase-1 in RASF
Abraham et al., J Immunol 2006 (Inflammation) : To examine this question, we sequentially measured LPS induced DNA binding of NF-kappaB in neutrophils collected from healthy humans on at least three occasions, each separated by at least 2 wk, and then determined pulmonary inflammatory responses after instillation of LPS into the lungs ... Consistent patterns of peripheral blood neutrophil responses, as determined by LPS induced NF-kappaB DNA binding, were present in volunteers, with a > 80-fold difference between individuals in the mean area under the curve for NF-kappaB activation
Wang et al., Br J Pharmacol 2006 (Hyperplasia) : 7. Thus, NF-kappaB activation during TMCH involves both IkappaB alpha degradation and p65-Ser536 phosphorylation ... 7. Thus, NF-kappaB activation during TMCH involves both IkappaB alpha degradation and p65-Ser536 phosphorylation
Yamada et al., J Biol Chem 2006 (Inflammation) : In addition, SP-A attenuated cell surface binding of smooth LPS and smooth LPS induced NF-kappaB activation in TLR4/MD-2 expressing cells ... Consistently significantly higher molar concentrations of CRF were required to inhibit smooth LPS induced NF-kappaB activation and tumor necrosis factor-alpha secretion
Jung et al., Biol Pharm Bull 2006 (Inflammation) : EMSA revealed that PGME of 5 and 50 microg/ml blocked the LPS stimulated activation of Nfkappab
Babbar et al., J Biol Chem 2006 (Carcinoma, Non-Small-Cell Lung...) : Here we show that TNFalpha can lead to the induction of NFkappaB signaling with a concomitant increase in spermidine/spermine N ( 1 ) -acetyltransferase ( SSAT ) expression in A549 and H157 non-small cell lung cancer cells ... Stable overexpression of a mutant, dominant negative IkappaBalpha protein led to the suppression of SSAT induction by TNFalpha in these cells, thereby substantiating a role of NFkappaB in the induction of SSAT by TNFalpha
Takahashi et al., J Vet Med Sci 2006 : These observations suggest that immediate response of NODs to endotoxins could result from NF-kappaB activation via TLR signaling, whereas the second rise in NOD mRNAs might have resulted from TNF-alpha production possibly through NF-kappaB, TLR, and/or NOD signalings
Chang et al., Cell Microbiol 2006 (Helicobacter Infections) : AP1 and cAMP response element ( CRE ), but not NF-kappaB , were involved in the induced cyclin D1 expression
Gutiérrez-Venegas et al., Eur J Pharmacol 2006 : Cell pretreatment with luteolin abolished LPS effects on NF-kappaB translocation
Begue et al., Gastroenterology 2006 (Disease Models, Animal...) : TRAIL induced activation of NF-kappaB was determined by electrophoretic mobility shift assay
Wong et al., Clin Immunol 2006 : Fas ( CD95 ) ligation inhibits activation of NF-kappa B by targeting p65-Rel A in a caspase dependent manner ... Fas ( CD95 ) ligation inhibits activation of NF-kappa B by targeting p65-Rel A in a caspase dependent manner ... Fas ( CD95 ) ligation inhibits activation of NF-kappa B by targeting p65-Rel A in a caspase dependent manner ... The inhibition is caspase dependent since an inhibitor of caspase activation, DEVD, can block the suppression of NF-kappaB activity following crosslinking of Fas
Wang et al., Diabetes Metab Res Rev 2007 (Diabetes Mellitus, Experimental) : ADMA ( 30 microM ) significantly increased the activity of NF-kappaB and elevated the levels of ICAM-1 and TNF-alpha , and pre-treatment with rosiglitazone ( 10 or 30 microM ) markedly inhibited the increased activity of NF-kappaB and reduced the elevated levels of TNF-alpha and ICAM-1 induced by ADMA in cultured endothelial cells ... ADMA ( 30 microM ) significantly increased the activity of NF-kappaB and elevated the levels of ICAM-1 and TNF-alpha, and pre-treatment with rosiglitazone ( 10 or 30 microM ) markedly inhibited the increased activity of NF-kappaB and reduced the elevated levels of TNF-alpha and ICAM-1 induced by ADMA in cultured endothelial cells
De Plaen et al., Immunology 2006 : It has been reported that enterocytes produce proinflammatory mediators, including tumour necrosis factor (TNF) and PAF, and we showed that lipopolysaccharide (LPS) and TNF activate nuclear factor (NF)-kappaB in enterocytes ... It has been reported that enterocytes produce proinflammatory mediators, including tumour necrosis factor (TNF) and PAF, and we showed that lipopolysaccharide (LPS) and TNF activate nuclear factor (NF)-kappaB in enterocytes ... LPS induced CXCL2 expression is dependent on NF-kappaB activation via the IKK pathway
Hardy et al., Mol Endocrinol 2006 (Inflammation) : In the present study, we tested the hypothesis that progesterone/PR inhibits uterine contractility by blocking nuclear factor kappaB (NF-kappaB) activation and induction of cyclooxygenase-2 (COX-2) , a contractile gene that is up-regulated in labor ... These effects were observed in the absence of exogenous progesterone, suggesting a ligand independent action of PR. Based on these findings, we propose that PR may inhibit NF-kappaB activation of COX-2 gene expression and uterine contractility via ligand dependent and ligand independent mechanisms
Riedel et al., World J Gastroenterol 2006 (Inflammation) : Anti-inflammatory effects of bifidobacteria by inhibition of LPS induced NF-kappaB activation ... As shown with two of the six inhibition positive bifidobacteria, LPS induced inhibition of NF-kappaB activation was accompanied by a dose dependent decrease of interleukin 8 (IL-8) secretion and by lower mRNA levels for IL-8, TNF-alpha, cyclooxygenase 2 (Cox-2), and intercellular adhesion molecule 1 ( ICAM-1 )
Park et al., Carcinogenesis 2007 (Neoplasms) : PMA selectively causes the degradation of IkappaB kinases ( IKKs ) including IKK-gamma and IKK-beta, and subsequent inhibition of tumor necrosis factor (TNF) induced IKK and NF-kappaB activation in human colon cancer cell line HCT-116, but not in other gastrointestinal tract cells
Hellerbrand et al., Carcinogenesis 2007 (Carcinoma, Hepatocellular...) : Recent studies suggested a role for CYLD in nuclear factor-kappaB (NF-kappaB) regulation
Rasoulpour et al., J Androl 2006 : The Sycp1-Cre transgenic mouse and male germ cell inhibition of NF-kappa b
Hu et al., Cell cycle (Georgetown, Tex.) 2006 : Furthermore, cIAP2-MALT1 and Bcl10 synergistically activate NF-kappaB ... Furthermore, cIAP2-MALT1 and Bcl10 synergistically activate NF-kappaB ... Furthermore, cIAP2-MALT1 and Bcl10 synergistically activate NF-kappaB
Sehnert et al., Arthritis Res Ther 2006 : Concomitantly, ALP suppressed the IL-1beta induced NF-kappaB activation and the upregulation of E-selectin expression in glEND.2 cells in vitro ... Concomitantly, ALP suppressed the IL-1beta induced NF-kappaB activation and the upregulation of E-selectin expression in glEND.2 cells in vitro
Hein et al., Clin Chim Acta 2006 (Bone Resorption...) : The AGE-RAGE interaction additionally induces activation of nuclear factor kB (NF-kB) in RAGE bearing cells ( e.g., cells participating in bone turnover ) ... The AGE-RAGE interaction additionally induces activation of nuclear factor kB (NF-kB) in RAGE bearing cells ( e.g., cells participating in bone turnover )
Han et al., Gut 2007 (Colitis) : Growth hormone activated STAT5, and directly reduced TNFalpha activation of NFkappaB , in T84 cells ... Growth hormone activated STAT5, and directly reduced TNFalpha activation of NFkappaB , in T84 cells
Tahera et al., Endocrinology 2006 (Hearing Loss, Noise-Induced) : RU486 + MET caused a simultaneous decreased I kappaB expression and NF kappaB nuclear translocation, demonstrating an interference with the I kappaB mediated activation of NF kappaB
Ralstin et al., Mol Cancer Res 2006 (Carcinoma, Hepatocellular) : The response of PLC cells to NS398 was augmented by p65 small interfering RNA to inhibit NF-kappaB p65 protein expression
Nagamatsu et al., J Pharmacol Sci 2006 : However, both IL-1beta and IL-6 mRNA induction was suppressed by nuclear factor kappaB (NF-kappaB) inhibitors ... The CA-G alpha13 induced NF-kappaB activation was suppressed by DPI and BAPTA-AM, but not C3 toxin and the Rho-kinase inhibitor Y27632
Wang et al., Life Sci 2006 : In addition, Casp9-gamma does not enhance NF-kappaB activation in transfected 293T cells, conflicting with previous evidence that the isolated CARD of caspase-9 activates NF-kappaB in ND7 cells
Rhee et al., Mol Immunol 2007 : These results lead us to a conclusion that NF-kappaB activation is required for MMP-9 gene expression ... In summary, our data suggest that NF-kappaB dependent expression of MMP-9 in response to CpG-ODN plays an important role in the recruitment of immune cells
Oh et al., Free Radic Biol Med 2006 : In addition, LPS induced nuclear factor (NF)-kappaB activation was diminished in RAW264.7 macrophages preincubated with H ( 2 ) S. Interestingly, the inhibitory effect of H ( 2 ) S on NF-kappaB activation was reversed by the transient transfection with HO-1 siRNA, but was mimicked by either HO-1 gene transfection or treatment with carbon monoxide ( CO ), an end product of HO-1
Song et al., Biochem Biophys Res Commun 2006 (Insulin Resistance) : In addition, activation of TLR4 with either LPS or free fatty acids stimulated NFkappaB signaling and expression of inflammatory cytokine genes, such as TNFalpha and IL-6 in 3T3-L1 adipocytes
Weldon et al., J Nutr Biochem 2007 (Inflammation) : Both EPA and DHA down-regulated LPS induced NF-kappaB/DNA binding in THP-1 macrophages by approximately 13 % ( P < or =.03 )
Li et al., Int Immunopharmacol 2006 (Lupus Erythematosus, Systemic) : The results demonstrated that DHA decreased the expression of NF-kappaB subunit p65 protein and the activation of NF-kappaB in the renal tissue of BXSB mice in vivo
Orrington-Myers et al., Am J Physiol Lung Cell Mol Physiol 2006 : In addition, DeltaEXD transfection suppressed LPS induced activation of NF-kappaB and consequent ICAM-1 expression
Chen et al., Shock 2006 (Burns...) : Taken together with the TNF-alpha dependent p38 and NF-kappaB activation in primed neutrophil, we conclude that thermal injury induced priming effect of polymorphonuclear neutrophil is TNF-alpha and p38 dependent
Lee et al., Planta Med 2006 : In this study, we found that pinosylvin, a natural stilbenoid that is a component of the pine leaf ( Pinus densiflora ), significantly inhibited LPS induced NF-kappaB activation in a concentration dependent manner
Jin et al., Planta Med 2006 : Polyozellin inhibits nitric oxide production by down regulating LPS induced activity of NF-kappaB and SAPK/JNK in RAW 264.7 cells ... Polyozellin suppressed the activation of both LPS induced NF-kappaB and the stress activated protein kinase ( SAPK ) /c-Jun N-terminal kinase (JNK), but had no effect on the extracellular signal regulated kinase ( ERK ) or p38
Kanda et al., Biochem Pharmacol 2006 : Anti-prostaglandin E2 ( PGE2 ) antiserum or antisense oligonucleotides against PGE2 receptor EP2 or EP3 abrogated inhibitory effects of ketoconazole and terbinafine hydrochloride on TNF-alpha induced NF-kappaB activity and CCL27, CCL2, and CCL5 production, indicating the involvement of endogenous PGE2 in the inhibitory effects ... Carboxyheptyl imidazole also suppressed TNF-alpha induced NF-kappaB activity and CCL27, CCL2, and CCL5 production ... These results suggest that ketoconazole and terbinafine hydrochloride may suppress TNF-alpha induced NF-kappaB activity and CCL27, CCL2, and CCL5 production by increasing PGE2 release from keratinocytes
Huerta-Yepez et al., Clin Immunol 2006 (Adenocarcinoma...) : Thus, we hypothesized that tumor derived TNF-alpha induces the activation of NF-kappaB and the transcription repressor YY1, both of which negatively regulate Fas expression and sensitivity to FasL induced apoptosis
Moon et al., Free Radic Biol Med 2006 : Results were supported by NF-kappaB activation as judged by reduced IkappaBalpha degradation and increased nuclear translocation of p65/RelA with LPS stimulation, serum deprivation, and PrxII deletion ... Results were supported by NF-kappaB activation as judged by reduced IkappaBalpha degradation and increased nuclear translocation of p65/RelA with LPS stimulation, serum deprivation, and PrxII deletion
Sethi et al., J Biol Chem 2006 : As examined by the DNA binding of NF-kappaB, we found that indirubin suppressed tumor necrosis factor (TNF) induced NF-kappaB activation in a dose- and time dependent manner ... NF-kappaB reporter activity induced by TNFR1 , TNF receptor associated death domain, TRAF2, TAK1, NF-kappaB inducing kinase, and IKKbeta was inhibited by indirubin but not that induced by p65 transfection ... NF-kappaB reporter activity induced by TNFR1, TNF receptor associated death domain, TRAF2 , TAK1, NF-kappaB inducing kinase, and IKKbeta was inhibited by indirubin but not that induced by p65 transfection ... NF-kappaB reporter activity induced by TNFR1, TNF receptor associated death domain, TRAF2, TAK1 , NF-kappaB inducing kinase, and IKKbeta was inhibited by indirubin but not that induced by p65 transfection ... NF-kappaB reporter activity induced by TNFR1, TNF receptor associated death domain, TRAF2, TAK1, NF-kappaB inducing kinase, and IKKbeta was inhibited by indirubin but not that induced by p65 transfection
Rallabhandi et al., J Immunol 2006 : Coexpression of WT TLR4, CD14, and MD-2 expression vectors in HEK293T cells was first optimized to achieve optimal LPS induced NF-kappaB reporter gene expression
Huang et al., J Immunol 2006 (Keratitis...) : SIGIRR also negatively regulated IL-1 and LPS, but not poly ( I:C ) -mediated signaling and NF-kappaB activation
Zwergal et al., J Immunol 2006 : C/EBP beta blocks p65 phosphorylation and thereby NF-kappa B-mediated transcription in TNF-tolerant cells ... Earlier experiments in TNF-tolerant cells display inhibition of NF-kappaB dependent IL-8 gene expression at the transcriptional level with potential involvement of C/EBPbeta ... Furthermore, in tolerant cells, TNF induced NF-kappaB p65 phosphorylation was markedly decreased, which was accompanied by the formation of C/EBPbeta-p65 complexes
Chen et al., J Immunol 2006 : Peptidoglycan induced IL-6 production in RAW 264.7 macrophages is mediated by cyclooxygenase-2, PGE2/PGE4 receptors, protein kinase A, I kappa B kinase, and NF-kappa B ... These results suggest that PGN induced IL-6 production involves COX-2 generated PGE ( 2 ), activation of the EP2 and EP4 receptors, cAMP formation, and the activation of PKA, protein kinase C, p38 MAPK, IKKdbeta, kinase alphabeta, p65 phosphorylation, and NF-kappaB
Ji et al., Ophthalmologica. Journal international d'ophtalmologie. International journal of ophthalmology. Zeitschrift für Augenheilkunde 2006 (Disease Models, Animal...) : To investigate whether pentoxifylline ( PTX ) could influence the increased cytokine gene expression in the retina flowing transient ischemia, and if so, whether it acts through the modulation of nuclear factor kappa B (NF-kappaB) activation
Malhi et al., Oncogene 2006 (Disease Progression...) : In these cells, the predominant effect of TRAIL receptor activation is the activation of nuclear factor-kappaB (NF-kappaB) , which promotes tumor metastases and invasion
Cao et al., Cell Res 2006 (Ovarian Neoplasms) : Although glycogen synthase kinase-3 ( GSK-3 ) might act as a tumor suppressor since its inhibition is expected to mimic the activation of Wnt signaling pathway, GSK-3beta may contribute to NF-kappaB activation in cancer cells leading to increased cancer cell proliferation and survival
Griffin et al., Biochem J 2006 : Furthermore, in contrast with IkappaBalpha, NF-kappaB activation did not increase expression of endogenous IkappaBbeta as assessed by analysis of mRNA and protein levels
Kim et al., Toxicology 2006 : p38 Mitogen activated protein kinase up-regulates LPS induced NF-kappaB activation in the development of lung injury and RAW 264.7 macrophages ... Furthermore, SB203580 blocked LPS induced NF-kappaB activation in lung tissue through down-regulation of serine phosphorylation, degradation of IkappaB-alpha, and consequent translocation of the p65 subunit of NF-kappaB to the nucleus ... It is likely that, in cultured RAW 264.7 macrophages, SB203580 also blocked LPS induced NF-kappaB activation in a dose dependent manner ... SB203580 inhibited LPS induced serine phosphorylation, degradation of IkappaB-alpha, and tyrosine phosphorylation of p65 NF-kappaB ... These data indicate that p38 MAPK acts upstream of LPS induced NF-kappaB activation by modulating the phosphorylation of IkappaB-alpha and p65 NF-kappaB during acute lung injury
Lin et al., J Biol Chem 2006 : Here, we show that the receptor interacting protein ( RIP ), a key mediator of tumor necrosis factor induced NF-kappaB and JNK activation, plays a key role in IGF-I receptor signaling
Nanua et al., Am J Respir Cell Mol Biol 2006 : Quercetin also inhibited TNF-alpha induced PI 3-kinase activity, Akt phosphorylation, intracellular H ( 2 ) O ( 2 ) production, NF-kappaB transactivation , IL-8 promoter activity, and steady-state mRNA levels, consistent with the notion that quercetin inhibits chemokine expression by attenuating NF-kappaB transactivation via a PI 3-kinase/Akt dependent pathway ... Quercetin also inhibited TNF-alpha induced PI 3-kinase activity, Akt phosphorylation, intracellular H ( 2 ) O ( 2 ) production, NF-kappaB transactivation , IL-8 promoter activity, and steady-state mRNA levels, consistent with the notion that quercetin inhibits chemokine expression by attenuating NF-kappaB transactivation via a PI 3-kinase/Akt dependent pathway ... Quercetin also inhibited TNF-alpha induced PI 3-kinase activity, Akt phosphorylation, intracellular H ( 2 ) O ( 2 ) production, NF-kappaB transactivation , IL-8 promoter activity, and steady-state mRNA levels, consistent with the notion that quercetin inhibits chemokine expression by attenuating NF-kappaB transactivation via a PI 3-kinase/Akt dependent pathway ... Quercetin also inhibited TNF-alpha induced PI 3-kinase activity, Akt phosphorylation, intracellular H ( 2 ) O ( 2 ) production, NF-kappaB transactivation , IL-8 promoter activity, and steady-state mRNA levels, consistent with the notion that quercetin inhibits chemokine expression by attenuating NF-kappaB transactivation via a PI 3-kinase/Akt dependent pathway
Jang et al., Eur J Pharmacol 2006 : Tanshinone IIA inhibits LPS induced NF-kappaB activation in RAW 264.7 cells : possible involvement of the NIK-IKK, ERK1/2, p38 and JNK pathways ... These results suggest that tanshinone IIA may inhibit LPS induced IkappaB alpha degradation and NF-kappaB activation via suppression of the NIK-IKK pathway as well as the MAPKs ( p38, ERK1/2, and JNK ) pathway in RAW 264.7 cells and these properties may provide a potential mechanism that explains the anti-inflammatory activity of tanshinone IIA
Shen et al., J Biol Chem 2006 : These findings were strikingly similar to the IL-6 promoter, where IL-17 mediated regulation of both NF-kappaB and C/EBP is essential
LaVallie et al., J Biol Chem 2006 (Osteoarthritis) : Protein kinase Czeta is up-regulated in osteoarthritic cartilage and is required for activation of NF-kappaB by tumor necrosis factor and interleukin-1 in articular chondrocytes ... Protein kinase Czeta is up-regulated in osteoarthritic cartilage and is required for activation of NF-kappaB by tumor necrosis factor and interleukin-1 in articular chondrocytes ... Chondrocyte cell based assays were used to evaluate the contribution of PKCzeta activity in NF-kappaB activation and extracellular matrix degradation mediated by IL-1, TNF , or sphingomyelinase ... Chondrocyte cell based assays were used to evaluate the contribution of PKCzeta activity in NF-kappaB activation and extracellular matrix degradation mediated by IL-1 , TNF, or sphingomyelinase ... In primary chondrocytes, IL-1 and TNF-alpha caused an increase in NF-kappaB activity resulting in induction of aggrecanase-1 and aggrecanase-2 expression, with consequent increased proteoglycan degradation ... In primary chondrocytes, IL-1 and TNF-alpha caused an increase in NF-kappaB activity resulting in induction of aggrecanase-1 and aggrecanase-2 expression, with consequent increased proteoglycan degradation
Fukuda et al., Invest Ophthalmol Vis Sci 2006 : Neither IL-4 nor -13 affected the IL-1beta induced activation of NF-kappaB or the AP-1 component c-Jun
Wan et al., Nat Immunol 2006 : In mature thymocytes, TAK1 was required for interleukin 7-mediated survival and T cell receptor dependent activation of transcription factor NF-kappaB and the kinase Jnk ... In mature thymocytes, TAK1 was required for interleukin 7-mediated survival and T cell receptor dependent activation of transcription factor NF-kappaB and the kinase Jnk
Amiri et al., Oncogene 2006 (Melanoma) : In its inactive state, PARP-1 binds to the CXCL1 promoter in a sequence-specific manner and prevents binding of NF-kappaB ( p65/p50 ) to its element
Wang et al., Antimicrob Agents Chemother 2006 (Escherichia coli Infections...) : ART treatment did, however, block the NF-kappaB activation induced by CpG ODN, LPS , or heat killed E. coli
Buchholz et al., Cell Microbiol 2006 : The AP-1, NFIL6 ( C/EBPbeta ) and NFkappaB transcriptional regulatory sites of the IL-8 promoter and the host NFkappaB signalling pathway were necessary for IL-8 induction by C. trachomatis
Jun et al., Inflamm Bowel Dis 2006 (Body Weight...) : Pretreatment of human epithelial HT-29 cells with gliotoxin significantly blocked the I-kappaB degradation and NF-kappaB p65 nuclear translocation induced by tumor necrosis factor-alpha or IL-1beta ; these were parallel with the inhibition of IL-8 secretion and intercellular adhesion molecule-1 expression in the same cells ... Pretreatment of human epithelial HT-29 cells with gliotoxin significantly blocked the I-kappaB degradation and NF-kappaB p65 nuclear translocation induced by tumor necrosis factor-alpha or IL-1beta ; these were parallel with the inhibition of IL-8 secretion and intercellular adhesion molecule-1 expression in the same cells
Nangle et al., Urology 2006 (Diabetes Mellitus, Experimental) : Oxidative or glyco-oxidative stress induced activation of the transcription factor , nuclear factor (NF)-kappaB , is associated with the neurovascular complications of diabetes mellitus
Kim et al., Mol Immunol 2007 (Ovarian Neoplasms) : TNF-alpha induced IL-8 promoter activation that is inhibited by saxatilin treatment was dependent on activating protein-1 (AP-1) instead of nuclear factor-kappa B (NF-kappaB)
Jeon et al., J Ethnopharmacol 2006 : KIOM-79 inhibits LPS induced iNOS gene expression by blocking NF-kappaB/Rel and p38 kinase activation in murine macrophages
Kefaloyianni et al., Cell Signal 2006 (MAP Kinase Signaling System) : ERK1/2 and p38-MAPK signalling pathways, through MSK1, are involved in NF-kappaB transactivation during oxidative stress in skeletal myoblasts ... ERK1/2 and p38-MAPK signalling pathways, through MSK1, are involved in NF-kappaB transactivation during oxidative stress in skeletal myoblasts ... ERK1/2 and p38-MAPK signalling pathways, through MSK1, are involved in NF-kappaB transactivation during oxidative stress in skeletal myoblasts
Calleros et al., Cell Signal 2006 (MAP Kinase Signaling System) : Here, we found that inhibition of p38 MAPK with the specific inhibitor SB203580 blocked the increase in NFkappaB activity, IkappaBalpha degradation and p65/NFkappaB translocation to the nucleus induced by cholesterol depletion
Thompson et al., Am J Respir Cell Mol Biol 2006 (Asthma) : CysLT1 receptor engagement induces activator protein-1- and NF-kappaB dependent IL-8 expression
Ishiguro et al., Mol Cell Biol 2006 : Ca2+/calmodulin dependent protein kinase II is a modulator of CARMA1 mediated NF-kappaB activation ... CARMA1 and Bcl10 functionally interact and control NF-kappaB signaling downstream of the T-cell receptor ( TCR ) ... CARMA1 and Bcl10 functionally interact and control NF-kappaB signaling downstream of the T-cell receptor ( TCR ) ... Furthermore, CaMKII enhances CARMA1 induced NF-kappaB activation ... These results provide a novel function for CaMKII in TCR signaling and CARMA1 induced NF-kappaB activation
Jin et al., Biochem J 2006 : cIAP2 ( cellular inhibitor of apoptosis protein 2 ) is induced by NF-kappaB ( nuclear factor kappaB ) when cells need to respond quickly to different apoptotic stimuli ... Cell cycle dependent G2/M-phase-specific cIAP2 expression is enhanced by NF-kappaB activation, and selective down-regulation of cIAP2 causes cells blocked in mitosis with nocodazole to become susceptible to apoptosis, indicating that the G2/M-phase-specific expression of cIAP2 contributes to the survival of mitotically arrested cells
Zhang et al., Cell Biol Int 2006 : The present study has clearly demonstrated the ability of Hcy to elicit an inflammatory response in rat VSMCs by stimulation of IL-6 production and activation of NF-kappaB
Yoshida et al., Neurotoxicology 2007 : DNA binding activities of NF-kappaB in these cells induced by interleukin (IL)-1 or LPS were inhibited by 1-BP ... DNA binding activities of NF-kappaB in these cells induced by interleukin (IL)-1 or LPS were inhibited by 1-BP
Albor et al., J Biol Chem 2006 (Muscular Dystrophies, Limb-Girdle) : Trim32 expression increased the transcriptional activity of NFkappaB in epidermal keratinocytes, both under basal treatment and after UVB/TNFalpha treatment ... Conversely, Piasy inhibited NFkappaB activity under the same conditions and sensitized keratinocytes to apoptosis induced by TNFalpha and UVB
Tai et al., Cancer Res 2006 (Bone Marrow Neoplasms...) : Adhesion of MM1S and MCCAR multiple myeloma cell lines to KM104 BMSC line transfected with a luciferase reporter vector carrying the BAFF gene promoter ( BAFF-LUC ) significantly enhanced luciferase activity, suggesting that nuclear factor-kappaB (NF-kappaB) activation induced by multiple myeloma adhesion to BMSCs mediates BAFF up-regulation
Filipe-Santos et al., J Exp Med 2006 (Genetic Predisposition to Disease...) : IL-12 production was also impaired as the result of a specific defect in NEMO- and NF-kappaB/c-Rel mediated CD40 signaling after the stimulation of monocytes and dendritic cells by CD40L expressing T cells and fibroblasts, respectively ... They also demonstrate the importance of the T cell- and CD40L triggered, CD40-, and NEMO/NF-kappaB/c-Rel mediated induction of IL-12 by monocyte derived cells for protective immunity to mycobacteria in humans
Nozell et al., J Immunol 2006 (Astrocytoma) : IFN-gamma-inducible protein-10 and MCP-1 genes, also regulated by NF-kappaB , are unaffected by IFN-beta, and IFN-beta does not prevent the activation, nuclear migration, or binding of NF-kappaB p65 to the kappaB element of the IFN-gamma-inducible protein-10 promoter
Watanabe et al., J Immunol 2006 : In this study, we show that Gads, but not Grb2, is essential for CD28 mediated NF-kappaB activation , and its binding to CD28 requires the whole CD28 cytoplasmic domain in addition to the YMNM motif ... In this study, we show that Gads, but not Grb2 , is essential for CD28 mediated NF-kappaB activation, and its binding to CD28 requires the whole CD28 cytoplasmic domain in addition to the YMNM motif
Rosenstiel et al., Cell Microbiol 2006 (Chronic Disease...) : Expression of NOD1 and NOD2 significantly sensitized HEK293 cells to H. pylori induced NF-kappaB activation in a cag pathogenicity island ( cagPAI ) -dependent manner ... Expression of NOD1 and NOD2 significantly sensitized HEK293 cells to H. pylori induced NF-kappaB activation in a cag pathogenicity island ( cagPAI ) -dependent manner
Kataoka et al., Cell Microbiol 2006 : TLR2 ( N114A ), TLR2 ( N199A ) and TLR2 ( N414A ) as well as wild-type TLR2 induced NF-kappaB activation when stimulated with these ligands, whereas TLR2 ( N442A ) failed to induce NF-kappaB activation ... TLR2 ( N114A, N199A ), TLR2 ( N114A, N414A ) and, to a lesser extent, TLR2 ( N114A, N442A ), in which two N-linked glycans are speculated to be exposed to the concave surface of TLR2 solenoid, not only induce NF-kappaB activation but also are associated with wild-type TLR2 and TLR6 ... TLR2 ( N114A, N199A ), TLR2 ( N114A, N414A ) and, to a lesser extent, TLR2 ( N114A, N442A ), in which two N-linked glycans are speculated to be exposed to the concave surface of TLR2 solenoid, not only induce NF-kappaB activation but also are associated with wild-type TLR2 and TLR6
Chan et al., J Rheumatol 2006 : The GLN and CS combination abrogated IL-1 induced gene expression of iNOS, COX-2, mPGEs1, and NF-kB at all timepoints
Chen et al., Exp Eye Res 2006 (Translocation, Genetic) : GS inhibits the expression of the ICAM-1 gene in ARPE-19 cells stimulated with IL-1beta by blocking NF-kappaB subunit p65 translocation and by partially preventing IkappaB degradation
Xu et al., Am J Physiol Cell Physiol 2006 (MAP Kinase Signaling System) : Ribosomal S6 kinase-1 modulates interleukin-1beta induced persistent activation of NF-kappaB through phosphorylation of IkappaBbeta ... In conclusion, in the ERK signaling cascade, RSK1 is a key component that directly phosphorylates IkappaBbeta and contributes to the persistent activation of NF-kappaB by IL-1beta
Zhang et al., World J Gastroenterol 2006 (Acute Disease...) : Effect of Tetrandrine on LPS induced NF-kappaB activation in isolated pancreatic acinar cells of rat ... To investigate the effect of Tetrandrine ( Tet ) on LPS induced NF-kappaB activation and cell injury in pancreatic acinar cells and to explore the mechanism of Tetrandrine preventing LPS induced acinar cell injury ... To investigate the effect of Tetrandrine ( Tet ) on LPS induced NF-kappaB activation and cell injury in pancreatic acinar cells and to explore the mechanism of Tetrandrine preventing LPS induced acinar cell injury
Cao et al., J Biol Chem 2006 : Taken together, our studies demonstrate that NF-kappaB1 ( p50 ) homodimers can be transcriptional activators of IL-10
Graham et al., J Biol Chem 2006 : Analysis of differentiating epithelia showed that only well differentiated enterocytes activated the 4-kb long MLCK promoter in response to TNF, and consensus promoter reporters demonstrated that TNF induced NFkappaB activation decreased during differentiation while TNF induced AP-1 activation increased
Maroni et al., Carcinogenesis 2007 (Breast Neoplasms...) : HGF induces CXCR4 and CXCL12 mediated tumor invasion through Ets1 and NF-kappaB ... HGF induces CXCR4 and CXCL12 mediated tumor invasion through Ets1 and NF-kappaB ... HGF induces CXCR4 and CXCL12 mediated tumor invasion through Ets1 and NF-kappaB ... In HGF treated MCF-7 cells, the DNA binding of Ets1 activated by MAPK1/ERK1/2 transduction pathway as well as the DNA binding of NF-kappaB played a critical role in CXCR4 transcription and protein induction
Adli et al., J Biol Chem 2006 (Inflammation) : NF-kappaB activation downstream of lipopolysaccharide or cytokine stimulation is controlled by the IkappaB kinase complex , which contains IKKalpha and IKKbeta ... Here, we demonstrate that IKK-i/IKKepsilon is expressed in a number of cancer cells and is involved in regulating NF-kappaB activity through its ability to control basal/constitutive, but not cytokine induced, p65/RelA phosphorylation at Ser-536, a modification proposed to contribute to the transactivation function of NF-kappaB
Kammanadiminti et al., J Biol Chem 2006 (Colitis) : Treatment with SAP inhibited NF-kappaB activation induced by interleukin-1beta ; specifically, the NF-kappaB-DNA binding, nuclear translocation of p65 subunit, and phosphorylation of IkappaB-alpha were reduced ... Gene silencing by small interfering RNA confirmed the role of Hsp27 in suppressing NF-kappaB activation at IkappaB kinase (IKK) level ... Overexpression of wild type Hsp27 amplified the effects of SAP, whereas a phosphorylation-deficient mutant of Hsp27 abrogated SAP induced NF-kappaB inhibition
Konson et al., Cancer Gene Ther 2006 (Colonic Neoplasms) : Our current study aimed to investigate the involvement of nuclear factor-kappa B (NF-kappaB) , a pivotal transcriptional regulator of COX-2 , in the upregulation of COX-2 expression by HSV-tk
Zhou et al., J Clin Invest 2006 (Carcinoma, Hepatocellular...) : Interestingly, NF-kappaB activation reciprocally inhibits SXR and its target genes whereas inhibition of NF-kappaB enhances SXR activity
Alonso et al., J Pineal Res 2006 : Exercise caused a significant induction of iNOS protein levels and a marked activation of NF-kappaB that were significantly prevented in rats treated with melatonin
Lee et al., Neurosci Res 2006 : In addition, SG prevented LPS induced DNA binding and transcriptional activity of nuclear factor KappaB (NF)-kappaB
Kettner-Buhrow et al., Biochem Biophys Res Commun 2006 (MAP Kinase Signaling System) : dsiRNA against TAK1 or TAB1 significantly suppressed their target proteins as well as TAK1 mediated activation of NFkappaB , p38 MAPK, and JNK, and of IL-8 transcription
Kim et al., Biochem Biophys Res Commun 2006 (Glioma) : C6 matrices potentiated LPS induced NF-kappaB DNA binding/transcriptional activity, suggesting that the suppression of LPS induced iNOS by C6 matrices is NF-kappaB independent
Zambre et al., Bioorg Med Chem 2006 (Leukemia) : Novel curcumin analogs targeting TNF induced NF-kappaB activation and proliferation in human leukemic KBM-5 cells ... Copper ( II ) conjugates of all synthesized ligands were prepared and structurally characterized as well as evaluated for their potential of inhibiting TNF induced NF-kappaB activation and proliferation in human leukemic KBM-5 cells wherein compound 13 was found to be more potent than curcumin
Ueno et al., Cardiovasc Toxicol 2006 (Atherosclerosis) : Inhibitors of p38 MAPK, PKC, and NF-kappaB suppressed nicotine stimulated expression of ICAM-1 and VCAM-1 ... Inhibitors of p38 MAPK, PKC, and NF-kappaB suppressed nicotine stimulated expression of ICAM-1 and VCAM-1 ... Our results indicate that nicotine enhances the expression of ICAM-1 and VCAM-1 on the endothelial cell surface via a second messenger pathway which involves PKC and p38 MAPK mediated activation of NF-kappaB and AP-1, resulting in increased expression of these cellular adhesion molecules
Ozaki et al., Nat Genet 2006 (Myocardial Infarction) : Moreover, suppression of PSMA6 expression using short interfering RNA in cultured cells reduced activation of the transcription factor NF-kappaB by stabilizing phosphorylated IkappaB
Brandon et al., J Vet Pharmacol Ther 2006 (Endotoxemia...) : Lastly, NF-kappaB reporter gene assays revealed an IB-MECA concentration dependent inhibition of TNFalpha stimulated NF-kappaB activity
Jiang et al., Yao Xue Xue Bao 2006 : T-614 inhibited LPS stimulated mRNA expression and production of TNFalpha in a concentration dependent manner, as well as the activity of NF-kappaB ... The inhibitory effect of T-614 on the production of TNFalpha in LPS stimulated NR8383 cells may be mediated by suppression of NF-kappaB activity
Parsa et al., PLoS Pathog 2006 (Gram-Negative Bacterial Infections) : Inhibition of PI3K/Akt resulted in suppression of F. novicida induced cytokine production through the inhibition of NFkappaB ... Inhibition of PI3K/Akt resulted in suppression of F. novicida induced cytokine production through the inhibition of NFkappaB
Farhangkhoee et al., Nutrition & metabolism 2006 : Cultured MVECs, exposed either to glucose or glucose and varying concentrations of curcumin, were assessed for alterations of NOS expression and activation of nuclear factor-kappaB (NF-kappaB) and activating protein-1 (AP-1)
Hayashi et al., J Immunol 2006 : Depletion of FLNa by RNA interference did not affect TCR induced early tyrosine phosphorylation or actin polymerization but, nevertheless, resulted in impaired IL-2 expression by human primary T cells and reduced activation of NF-kappaB , AP-1, and NFAT reporter genes in transfected T cells ... Depletion of FLNa by RNA interference did not affect TCR induced early tyrosine phosphorylation or actin polymerization but, nevertheless, resulted in impaired IL-2 expression by human primary T cells and reduced activation of NF-kappaB , AP-1, and NFAT reporter genes in transfected T cells
Nakashima et al., Cancer Res 2006 (Carcinoma, Pancreatic Ductal...) : We hypothesized that nuclear factor-kappaB (NF-kappaB) , a hallmark transcription factor in inflammatory responses, contributes to the overexpression of Shh in pancreatic cancer ... NF-kappaB induced Shh expression actually activated the Hh pathway in a ligand dependent manner and enhanced cell proliferation in pancreatic cancer cells
Dulos et al., Ann N Y Acad Sci 2006 (Arthritis) : The results show a role for nuclear factor kappa B (NFkappaB) and activator protein-1 (AP-1) in the regulation of Cyp7b expression
Zaragoza et al., Biol Reprod 2006 : Inhibition of NFkappaB activation with either the proteasome inhibitor MG132 or phenethyl caffeiate reduced PTGFR mRNA levels, which indicates that this transcription factor is important for basal transcription ... These results are consistent with the proposal that proinflammatory cytokines directly regulate uterine activation genes and that the transcription factor NFkappaB is involved in both basal and IL1beta stimulated transcription of the PTGFR gene
Wang et al., Asian J Androl 2006 : NF-kappa B is activated by increased FasL/Fas in CORT induced Leydig cell apoptosis ... NF-kappa B is activated by increased FasL/Fas in CORT induced Leydig cell apoptosis
Djavaheri-Mergny et al., J Biol Chem 2006 : This repression is associated with an NF-kappaB dependent activation of the autophagy inhibitor mTOR
Lemarié et al., Circ Res 2006 : Here we report that high intraluminal pressure induces reactive oxygen species ( ROS ) in arteries and that inhibition of NADPH oxidase prevents both the generation of ROS and the activation of NF-kappaB associated with high pressure
Farley et al., J Invest Dermatol 2006 (Dermatitis...) : In HaCaT cells and in reconstructed human epidermis ( RHE ), FasL triggered a NF-kappaB dependent mRNA accumulation of inflammatory cytokines ( tumor necrosis factor-alpha , IL-6, and IL-1beta ), chemokines ( CCL2/MCP-1, CXCL1/GROalpha, CXCL3/GROgamma, and CXCL8/IL-8 ), and the adhesion molecule ICAM-1 ... In HaCaT cells and in reconstructed human epidermis ( RHE ), FasL triggered a NF-kappaB dependent mRNA accumulation of inflammatory cytokines ( tumor necrosis factor-alpha, IL-6, and IL-1beta ), chemokines ( CCL2/MCP-1, CXCL1/GROalpha, CXCL3/GROgamma, and CXCL8/IL-8 ), and the adhesion molecule ICAM-1 ... In HaCaT cells and in reconstructed human epidermis ( RHE ), FasL triggered a NF-kappaB dependent mRNA accumulation of inflammatory cytokines ( tumor necrosis factor-alpha, IL-6, and IL-1beta ), chemokines ( CCL2/MCP-1, CXCL1/GROalpha, CXCL3/GROgamma, and CXCL8/IL-8 ), and the adhesion molecule ICAM-1 ... In HaCaT cells and in reconstructed human epidermis ( RHE ), FasL triggered a NF-kappaB dependent mRNA accumulation of inflammatory cytokines ( tumor necrosis factor-alpha, IL-6 , and IL-1beta ), chemokines ( CCL2/MCP-1, CXCL1/GROalpha, CXCL3/GROgamma, and CXCL8/IL-8 ), and the adhesion molecule ICAM-1
Zhang et al., Infect Immun 2006 (Actinobacillus Infections...) : Interleukin-10 inhibits gram-negative-microbe-specific human receptor activator of NF-kappaB ligand positive CD4+-Th1-cell associated alveolar bone loss in vivo
Fribley et al., Cancer Biol Ther 2006 (Neoplasms) : Activation of the transcription factor nuclear factor kappa B (NF-kappaB) , a key survival factor, is dependent on the 26S proteasome
Gross et al., Nature 2006 : Card9 couples to Bcl10 and regulates Bcl10-Malt1 mediated NF-kappaB activation induced by zymosan ... Card9 couples to Bcl10 and regulates Bcl10-Malt1 mediated NF-kappaB activation induced by zymosan ... Card9 couples to Bcl10 and regulates Bcl10-Malt1 mediated NF-kappaB activation induced by zymosan
Chua et al., Oncogene 2007 (Cell Transformation, Neoplastic...) : NF-kappaB represses E-cadherin expression and enhances epithelial to mesenchymal transition of mammary epithelial cells : potential involvement of ZEB-1 and ZEB-2 ... Using parental MCF10A cells and a variant that expresses the myoepithelial marker p63 stably overexpressing the constitutively active p65 subunit of NF-kappaB ( MCF10A/p65 ), we show that NF-kappaB suppresses the expression of epithelial specific genes E-cadherin and desmoplakin and induces the expression of the mesenchymal specific gene vimentin
Han et al., Free Radic Biol Med 2006 : These redox alterations were found to inhibit TNF induced IkappaB-alpha phosphorylation and NF-kappaB translocation to the nucleus, thus presumably inhibiting expression of genes necessary to inhibit the cytotoxic effects of TNF ... The TNF induced apoptosis seems to occur only in a certain redox range -- in which redox changes can inhibit NF-kappaB activity but not completely inhibit caspase activity
Nishimura et al., Int J Oncol 2006 (Carcinoma, Hepatocellular...) : DHMEQ blocked the constitutive DNA binding activity and TNF-alpha mediated nuclear translocation of NF-kappaB in Huh-7 cells
Patel et al., Biochem Biophys Res Commun 2006 : LPS stimulated both AP-1 ( c-Fos, c-Jun ) and NF-kappaB ( p50 and p65 ) activation, but only inhibition of AP-1 attenuated LPS induced CXCR6 expression ... LPS stimulated both AP-1 ( c-Fos, c-Jun ) and NF-kappaB ( p50 and p65 ) activation, but only inhibition of AP-1 attenuated LPS induced CXCR6 expression
Kim et al., Biochem Biophys Res Commun 2006 : Involvement of HDAC1 and the PI3K/PKC signaling pathways in NF-kappaB activation by the HDAC inhibitor apicidin ... Involvement of HDAC1 and the PI3K/PKC signaling pathways in NF-kappaB activation by the HDAC inhibitor apicidin ... Involvement of HDAC1 and the PI3K/PKC signaling pathways in NF-kappaB activation by the HDAC inhibitor apicidin ... In addition, our data show that the early expression of TNF-alpha does not lead to activation of NF-kappaB , because disruption of TNF-alpha activity by a neutralizing antibody does not affect nuclear translocation of NF-kappaB, IkappaBalpha degradation or reporter gene activation by apicidin ... However, this activation of NF-kappaB requires the PI3K and PKC signaling pathways, but not ERK or JNK ... Furthermore, apicidin activation of NF-kappaB seems to result from HDAC1 inhibition, as evidenced by the observation that overexpression of HDAC1, but not HDAC2 , 3 or 4, dramatically inhibits NF-kappaB reporter gene activity ... Furthermore, apicidin activation of NF-kappaB seems to result from HDAC1 inhibition, as evidenced by the observation that overexpression of HDAC1 , but not HDAC2, 3 or 4, dramatically inhibits NF-kappaB reporter gene activity ... Collectively, our results suggest that activation of NF-kappaB signaling by apicidin requires both the PI3K/PKC signaling pathways and HDAC1 , and functions as a critical modulator in determining the cellular effect of apicidin
Eisner et al., FEBS Lett 2006 : Hyperosmotic stress dependent NFkappaB activation is regulated by reactive oxygen species and IGF-1 in cultured cardiomyocytes ... NFkappaB activation and procaspase-3 and -9 fragmentation were prevented by NAC and IGF-1 ... NFkappaB activation and procaspase-3 and -9 fragmentation were prevented by NAC and IGF-1 ... IGF-1 prevents NFkappaB activation by a ROS independent mechanism
Trevino et al., Angiogenesis 2006 (Adenocarcinoma...) : Pharmacologic inhibition of NF-kappaB activity significantly reduced basal IL-8 expression and tumor necrosis factor induced IL-8 expression ( P < 0.05 for both ), yet NF-kappaB activity was not dependent on Src ... Pharmacologic inhibition of NF-kappaB activity significantly reduced basal IL-8 expression and tumor necrosis factor induced IL-8 expression ( P < 0.05 for both ), yet NF-kappaB activity was not dependent on Src ... We therefore suggest that Src activation, through phosphorylation of STAT3, and NF-kappaB are all required for expression of IL-8 a critical angiogenic promoting factor in pancreatic adenocarcinomas
Thirunavukkarasu et al., Hepatology 2006 (Disease Models, Animal...) : Inhibition of p38 and NF-kappaB activation by SB203580 and pyrrolidine dithiocarbamate, respectively, blocked endotoxin induced H ( 2 ) O ( 2 ), NO, TNF-alpha , and IL-6 synthesis ... Inhibition of p38 and NF-kappaB activation by SB203580 and pyrrolidine dithiocarbamate, respectively, blocked endotoxin induced H ( 2 ) O ( 2 ), NO, TNF-alpha, and IL-6 synthesis ... In conclusion, endotoxin induced synthesis of NO, TNF-alpha , and IL-6 in HSCs is mediated by p38 and NF-kappaB , with involvement of H ( 2 ) O ( 2 ) in TNF-alpha production ... In conclusion, endotoxin induced synthesis of NO, TNF-alpha, and IL-6 in HSCs is mediated by p38 and NF-kappaB , with involvement of H ( 2 ) O ( 2 ) in TNF-alpha production
Laza-Stanca et al., J Virol 2006 (Asthma...) : Rhinovirus replication in human macrophages induces NF-kappaB dependent tumor necrosis factor alpha production ... Finally, we established the requirement for NF-kappaB but not p38 mitogen activated protein kinase in induction of TNF-alpha
Shen et al., FASEB J 2006 : Recently, some important discoveries have underscored the critical role of ROS in TNFalpha signaling, notably in TNFalpha mediated activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase ( c-Jun NH2-terminal kinase, JNK ), as well as in cell death ( apoptotic and necrotic ) pathways ... Here we attempt to review the existing knowledge on the involvement of ROS in death receptor signaling using TNFalpha-TNFR1 as the model system, specifically addressing the involvement of intracellular ROS in TNFalpha induced cell death and in TNFalpha induced activation of NF-kappaB and JNK and their crosstalk
Kim et al., EMBO J 2006 : Upon stimulation of the cells by TNF-alpha , NF-kappaB and TFIIH are rapidly recruited to the promoter together with additional Mediator and RNAP II, but CDK8 is lost
Yoshida et al., Life Sci 2006 : PEDF blocked the IL-6 induced STAT3 phosphorylations and NF-kappaB p65 activity in Hep3B cells ... PEDF blocked the IL-6 induced STAT3 phosphorylations and NF-kappaB p65 activity in Hep3B cells
Faith et al., Antiviral Res 2006 : Furthermore, resveratrol did not protect IkappaBalpha , a cytoplasmic NF-kappaB inhibitor , from degradation in HSV-1 infected cells
Kuo et al., Oncogene 2007 : Furthermore, 32D/IRS-1/ALK cells display an enhanced activation of mitogen activated protein kinase and PI3-kinase pathways, and a selective transcriptional activation of nuclear factor (NF)-kappaB ... Furthermore, 32D/IRS-1/ALK cells display an enhanced activation of mitogen activated protein kinase and PI3-kinase pathways, and a selective transcriptional activation of nuclear factor (NF)-kappaB ... We conclude that the recruitment of IRS-1 to activated ALK and the activation of NF-kappaB are essential for the autocrine growth and survival signaling of MK
Hayashi et al., Journal of thrombosis and haemostasis : JTH 2006 : Lipopolysaccharide induced decreased protein S expression in liver cells is mediated by MEK/ERK signaling and NFkappaB activation : involvement of membrane bound CD14 and toll-like receptor-4 ... Lipopolysaccharide induced decreased protein S expression in liver cells is mediated by MEK/ERK signaling and NFkappaB activation : involvement of membrane bound CD14 and toll-like receptor-4 ... Antirat CD14 and antirat TLR-4 antibodies inhibited LPS induced NFkappaB activation, and a NFkappaB inhibitor suppressed LPS induced decreased PS expression in both cells ... Antirat CD14 and antirat TLR-4 antibodies inhibited LPS induced NFkappaB activation, and a NFkappaB inhibitor suppressed LPS induced decreased PS expression in both cells
Ulfhammer et al., Journal of thrombosis and haemostasis : JTH 2006 (Cardiovascular Diseases...) : Further, we provide evidence for a TNF-alpha induced binding of NF-kappaB to the recently described kappaB site in the t-PA gene and of cyclic adenosine monophosphate response element binding protein ( CREB ) to the t-PA CRE-like site
Chowdhury et al., Clin Exp Immunol 2006 (Bacterial Infections) : The TLR2- and TLR4 mediated response to stimulation was dependent on NF-kappaB signalling, as shown by curcumin pretreatment of TECs
Ma et al., Am J Chin Med 2006 : This study was to investigate the inhibitive effect of resveratrol ( RESV ) on nuclear factor kappa B (NF-kappaB) expression and activity induced by lipopolysaccharide (LPS) in rat peritoneal macrophages ( PMA )
Taganov et al., Proc Natl Acad Sci U S A 2006 : NF-kappaB dependent induction of microRNA miR-146 , an inhibitor targeted to signaling proteins of innate immune responses
Gong et al., J Am Soc Nephrol 2006 (Chronic Disease...) : In addition, HGF suppressed NF-kappaB reporter gene activity that was induced by TNF-alpha and counteracted TNF-alpha elicited NF-kappaB interaction with kappaB elements at the E-selectin gene level ... In addition, HGF suppressed NF-kappaB reporter gene activity that was induced by TNF-alpha and counteracted TNF-alpha elicited NF-kappaB interaction with kappaB elements at the E-selectin gene level
Luan et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2006 (Acute Lung Injury...) : To investigate the possible effects of nuclear factor-KappaB (NF-KappaB) activation on the expression of intercellular adhesion molecular-1 ( ICAM-1 ) and P-selectin in murine acute lung injury ( ALI ) and assess the potential beneficial effects and mechanism of low molecular weight heparin ( LMWH ) and aspirin ( ASA )
Soloff et al., Mol Hum Reprod 2006 : Interleukin-1 induced NF-kappaB recruitment to the oxytocin receptor gene inhibits RNA polymerase II-promoter interactions in cultured human myometrial cells
Rice et al., J Vasc Res 2006 : OPN mediated NF-kappaB activity is increased upon NIK wild-type ( WT ) overexpression and blocked following NIK DN overexpression ... Finally, functional inhibition of integrin beta3 dependent NF-kappaB signaling decreases OPN induced IkappaBalpha, IKKbeta and NIK phosphorylation
Wieckowski et al., J Cell Biochem 2007 (Carcinoma, Squamous Cell...) : FAP-1 mediated activation of NF-kappaB induces resistance of head and neck cancer to Fas induced apoptosis ... Treatment with the phosphatase inhibitor, orthovanadate, or silencing of FAP-1 with siRNA abolished their resistance to apoptosis, suggesting that FAP-1 phosphatase activity could be responsible for NF-kappaB activation and resistance of SCCHN cells to Fas mediated apoptosis
Kang et al., Toxicol Appl Pharmacol 2006 : These results suggest that the 5d-PGJ2 induced OK cell death was associated with ROS production and NF-kappaB inhibition , but not with MAPK activation
Girvan et al., Mol Cancer Ther 2006 (Neoplasms) : AGRO100 inhibits activation of nuclear factor-kappaB (NF-kappaB) by forming a complex with NF-kappaB essential modulator (NEMO) and nucleolin ... AGRO100 inhibits activation of nuclear factor-kappaB (NF-kappaB) by forming a complex with NF-kappaB essential modulator (NEMO) and nucleolin ... In the classic NF-kappaB pathway, the IKK complex is required for phosphorylation of IkappaBalpha and subsequent activation of the transcription factor NF-kappaB ... In the classic NF-kappaB pathway, the IKK complex is required for phosphorylation of IkappaBalpha and subsequent activation of the transcription factor NF-kappaB
van Loo et al., Nat Immunol 2006 (Encephalomyelitis, Autoimmune, Experimental...) : NF-kappaB inhibition prevented the expression of proinflammatory cytokines, chemokines and the adhesion molecule VCAM-1 from CNS-resident cells
Kim et al., Eur J Immunol 2006 (Diarrhea) : Bacteroides fragilis enterotoxin induces cyclooxygenase-2 and fluid secretion in intestinal epithelial cells through NF-kappaB activation ... Suppression of the NF-kappaB signal significantly decreased COX-2 expression in response to BFT stimulation
Huangfu et al., J Biol Chem 2006 : Osmotic stress activates the TAK1-JNK pathway while blocking TAK1 mediated NF-kappaB activation : TAO2 regulates TAK1 pathways ... Furthermore, we found that although TAK1 was highly activated upon osmotic stress, it could not induce degradation of IkappaB or activation of NF-kappaB ... We found that TAO2 ( thousand-and-one amino acid kinase 2 ) associates with TAK1 and can inhibit TAK1 mediated activation of NF-kappaB but not of JNK
Kawakami et al., Circulation 2006 (Atherosclerosis...) : PKCbeta inhibition abolished apoCIII induced NF-kappaB activation, and NF-kappaB inhibition reduced expression of VCAM-1 , each resulting in reduced THP-1 cell adhesion ... PKCbeta inhibition abolished apoCIII induced NF-kappaB activation, and NF-kappaB inhibition reduced expression of VCAM-1, each resulting in reduced THP-1 cell adhesion
Jiang et al., Cell Res 2006 (Respiratory Distress Syndrome, Adult) : Lung epithelial cell overexpression of high molecular mass HA protected mice against acute lung injury and apoptosis, in part through TLR dependent basal activation of NF-kappaB
Fitau et al., Nephrol Ther 2005 : Western blot analysis showed that Lnk overexpression in HUVEC was associated with phosphorylation of Akt kinase ( at Ser 473 ) with no effect on IkappaBalpha , the specific inhibitor of NFkappaB , indicating that Lnk promotes activation of the phosphatidylinositol 3-kinase ( PI3-kinase ) pathway in ECs
Makata et al., Naunyn Schmiedebergs Arch Pharmacol 2006 (Mucocutaneous Lymph Node Syndrome) : High-dose IVIG inhibited tumor necrosis factor-alpha (TNF-alpha) induced activation of nuclear factor-kappaB (NF-kappaB) to a greater degree than dexamethasone in human monocytic U937 cells and human coronary arterial endothelial cells ( HCAEC ), but not in human T lymphocytic Jurkat cells
Lindbom et al., Inflammation 2005 : Furthermore, LPS ( but not IFN-gamma ) increased the mRNA levels of the nuclear factor (NF)-kappaB inhibitors alpha and xi in macrophages, but not in RPMI 2650 cells
Benson et al., Eur J Immunol 2006 : Rapid CD40 rescue did not require NF-kappaB activation and was independent of de novo protein synthesis, but was dependent upon active PI3 K
Kim et al., Eur J Pharmacol 2006 : Cordycepin inhibits lipopolysaccharide induced inflammation by the suppression of NF-kappaB through Akt and p38 inhibition in RAW 264.7 macrophage cells
Pace et al., Eur J Cancer 2006 (Adenocarcinoma...) : Moreover, exposure to exudative malignant, but not to CHF pleural fluids, increased the mRNA ( measured by real-time polymerase chain reaction ( PCR ) ) and protein expression of COX-2 ( measured by Western blot ), as well as the activation and nuclear translocation of nuclear factor kappaB (NFkappaB) in cancer cells
González-Pacheco et al., Am J Physiol Heart Circ Physiol 2006 : Wild-type and mutant inhibitors of kappaBalpha ( IkappaBalpha ) constructions were used to further assess the role of NF-kappaB in the induction of VEGFR2 expression ... Exposure to exogenous VEGF also increased VEGFR2 and induced NF-kappaB in EC
Fukuda et al., In Vivo 2006 (Carcinoma, Adenoid Cystic...) : CYLD has deubiquitinating enzyme activity and inhibits the activation of transcription factor NF-kappaB
Eeva et al., Mol Immunol 2007 : We conclude that the anti-apoptotic signaling of CD40 involves NF-kappaB mediated induction of c-FLIP proteins which can interfere with caspase-8 activation
Wu et al., Can J Physiol Pharmacol 2006 (Asthma) : Montelukast prevents the decrease of interleukin-10 and inhibits NF-kappaB activation in inflammatory airway of asthmatic guinea pigs ... In the present study, the effects of montelukast on the level of IL-10 and on the activation of NF-kappaB in the inflammatory airway of asthmatic guinea pigs were investigated ... There was a significantly negative correlation between the level of IL-10 and the activation of NF-kappaB in lung tissues ( r = -0.488, p < 0.01 ) ... From all these findings we conclude that montelukast can prevent the decrease of IL-10 and inhibit the activation of NF-kappaB in inflammatory airway of asthmatic guinea pigs, which may be the new important mechanisms of montelukast 's anti-airway-inflammation effects in asthmatic guinea pigs
Escobar et al., Surgery 2006 : Therefore, we investigated the role of clathrin heavy chain (CHC) in tumor necrosis factor alpha (TNF-alpha) induced IKB alpha phosphorylation and NFKB activation ... Therefore, we investigated the role of clathrin heavy chain (CHC) in tumor necrosis factor alpha (TNF-alpha) induced IKB alpha phosphorylation and NFKB activation
Mabb et al., Nat Cell Biol 2006 : PIASy mediates NEMO sumoylation and NF-kappaB activation in response to genotoxic stress ... SUMO-1 modification of NEMO ( NF-kappaB essential modulator ), the IkappaB kinase (IKK) regulatory subunit, is critical for activation of NF-kappaB by genotoxic agents
Ciucci et al., Mol Pharmacol 2006 (Breast Neoplasms) : Herein, we describe the NF-kappaB dependent anticancer activity of natural and synthetic cyclopentenone IKK inhibitors
Moulin et al., Biorheology 2006 : The inhibitory potency of 15d-PGJ2 was unaffected by changes in PPARgamma expression and resulted from inhibition of NF-kappaB nuclear binding and IkappaBalpha sparing, secondary to reduced phosphorylation of IKKbeta
Domagala et al., Biorheology 2006 : Inhibition of interleukin-1beta induced activation of MEK/ERK pathway and DNA binding of NF-kappaB and AP-1 : potential mechanism for Diacerein effects in osteoarthritis
Shi et al., Shock 2006 (MAP Kinase Signaling System) : Overexpression of HSP70 inhibited the nuclear translocation of p65, the transcriptionally active component of the NF-kappaB complex, and prevented the degradation of IkappaBalpha , the regulator of NF-kappaB activity
Fu et al., Mol Cell Biol 2006 : Parathyroid hormone controls receptor activator of NF-kappaB ligand gene expression via a distant transcriptional enhancer
Reynaert et al., Proc Natl Acad Sci U S A 2006 : Conversely, GRX1 knockdown sensitizes cells to oxidative inactivation of IKK-beta and dampens TNF-alpha induced IKK and NF-kappaB activation
Berclaz et al., Am J Respir Cell Mol Biol 2007 : LPS dependent NF-kappaB promoter activity, TNF-alpha secretion, and neutrophil chemokine release were reduced in AM cell lines derived from GM ( -/- ) mice ( mAM ) compared with GM ( +/+ ) ( MH-S )
Liu et al., EMBO J 2006 : Latent membrane protein 1 (LMP1) , which is an Epstein-Barr virus ( EBV ) -encoded oncoprotein, induces nuclear factor-kappa B (NF-kappaB) signaling by mimicking the tumor necrosis factor receptor (TNFR) ... Here, we identify a new LMP1 interacting molecule, prenylated Rab acceptor 1 (PRA1), which interacts with LMP1 for the first time through LMP1 's transmembrane domain, and show that PRA1 is involved in intracellular LMP1 trafficking and LMP1 induced NF-kappaB activity ... Immunofluorescence and biochemical analyses revealed that LMP1 physically interacted with PRA1 at the Golgi apparatus, and the colocalization of LMP1 and PRA1 to the Golgi was sensitive to nocodazole and brefeldin A. Coexpression of a PRA1 export mutant or knockdown of PRA1 led to redistribution of LMP1 and its associated signaling molecules to the endoplasmic reticulum and subsequent impairment of LMP1 induced NF-kappaB activation, but had no effect on CD40- and TNFR1 mediated signaling or the functional integrity of the Golgi apparatus
Westra et al., Mini Rev Med Chem 2006 (Arthritis, Rheumatoid) : In this review new insights such as non-stressful activation of p38 MAPK, and the role of p38 MAPK in regulation of NF-kappaB recruitment are also discussed
Aljada et al., Metabolism 2006 (Inflammation) : Glucose ingestion ( 75 g in 300 mL water ) in healthy human subjects resulted in an increase in intranuclear nuclear factor kappaB (NF-kappaB) binding, the reduction of inhibitor kappaB alpha ( IkappaBalpha ) protein, and an increase in the activity of inhibitor kappaB kinase (IKK) and the expression of IKKalpha and IKKbeta, the enzymes that phosphorylate IkappaBalpha, in MNCs. Glucose intake caused an increase in NF-kappaB binding to NF-kappaB2, NF-kappaB2a, and NF-kappaB3 sequences in the promoter site of tumor necrosis factor alpha (TNF-alpha) gene along with an increase in the expression of TNF-alpha messenger RNA in MNCs. Membranous p47(phox) subunit, an index of nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase expression and activation, also increased after glucose intake ... Glucose ingestion ( 75 g in 300 mL water ) in healthy human subjects resulted in an increase in intranuclear nuclear factor kappaB (NF-kappaB) binding, the reduction of inhibitor kappaB alpha ( IkappaBalpha ) protein, and an increase in the activity of inhibitor kappaB kinase (IKK) and the expression of IKKalpha and IKKbeta , the enzymes that phosphorylate IkappaBalpha, in MNCs. Glucose intake caused an increase in NF-kappaB binding to NF-kappaB2 , NF-kappaB2a, and NF-kappaB3 sequences in the promoter site of tumor necrosis factor alpha (TNF-alpha) gene along with an increase in the expression of TNF-alpha messenger RNA in MNCs. Membranous p47(phox) subunit, an index of nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase expression and activation, also increased after glucose intake
Na et al., Int Immunopharmacol 2006 (Endotoxemia) : 4-OMGA inhibited the LPS mediated increase in reactive oxygen species production and exogenous H ( 2 ) O ( 2 ) -induced NF-kappaB activation
Mo et al., J Ethnopharmacol 2007 : CML-1 inhibits TNF-alpha induced NF-kappaB activation and adhesion molecule expression in endothelial cells through inhibition of IkBalpha kinase
Bidère et al., Curr Biol 2006 : Caspase-8 regulation by direct interaction with TRAF6 in T cell receptor induced NF-kappaB activation ... Caspase-8 regulation by direct interaction with TRAF6 in T cell receptor induced NF-kappaB activation
Choi et al., Carcinogenesis 2007 (Prostatic Neoplasms) : The effect of SFN on levels of IAP family proteins as well as transcriptional activity of NFkappaB was biphasic in LNCaP cells
Perez et al., Am J Physiol Lung Cell Mol Physiol 2007 (Cystic Fibrosis...) : Continuous inhibition of CFTR-Cl ( - ) conductance for 3-5 days resulted in significant increase in IL-8 secretion at basal ( P = 0.006 ) and in response to 10 ( 9 ) Pseudomonas ( P = 0.0001 ), a fourfold decrease in Smad3 expression ( P = 0.02 ), a threefold increase in RhoA expression, and increased NF-kappaB nuclear translocation upon TNF-alpha/IL-1beta stimulation ( P < 0.000001 )
Steer et al., J Immunol 2006 (Cardiovirus Infections...) : Recently, we have shown that NF-kappaB is required for encephalomyocarditis virus ( EMCV ) - and dsRNA stimulated COX-2 expression in mouse macrophages
Gomez-Cabrera et al., Br J Nutr 2006 : In animal models, we previously showed that NF-kappaB activation induced by exhaustive physical exercise leads to an increase in the expression of superoxide dismutase , an enzyme involved in antioxidant defence
Zhou et al., J Biol Chem 2006 : Therefore, these results revealed the following roles of Ca2+ and PKC in the signaling pathway for NF-kappaB activation in LPS stimulated macrophages
Takada et al., Oncogene 2007 : We found that in wild-type mouse embryonic fibroblast (MEF), TNF induced NF-kappaB activation as measured by DNA binding but deletion of PKR abolished this activation
Ishiguro et al., Toxicol Appl Pharmacol 2006 (Colitis) : Furthermore, Paeonol reduced TNFalpha induced NF-kappaB transactivation and IFNgamma induced STAT1 transactivation in CW-2 cells and also in Jurkat cells ... Furthermore, Paeonol reduced TNFalpha induced NF-kappaB transactivation and IFNgamma induced STAT1 transactivation in CW-2 cells and also in Jurkat cells ... Furthermore, Paeonol reduced TNFalpha induced NF-kappaB transactivation and IFNgamma induced STAT1 transactivation in CW-2 cells and also in Jurkat cells
Hargett et al., J Virol 2006 : Beginning at 3 to 5 h postinfection, HSV-1 induces a robust and persistent nuclear translocation of an NF-kappaB dependent ( p50/p65 heterodimer ) DNA binding activity, as measured by electrophoretic mobility shift assay
Jang et al., Cell Immunol 2006 : The IL-4 induced ANT3 expression is dependent on tyrosine kinase, NF-kappaB , PI3K/Akt, and Erk pathways ... In fact, IL-4 induced specific activation of NF-kappaB , Akt, and Erk in Jurkat T cells and partially rescued these cells from dexamethasone induced apoptosis
Poole et al., EMBO J 2006 : The UL144 gene product of human cytomegalovirus activates NFkappaB via a TRAF6 dependent mechanism ... This NFkappaB activation enhances expression of the chemokine CCL22 through the NFkappaB responsive elements found in its promoter
Wullaert et al., Biochem Pharmacol 2006 : Mechanisms of crosstalk between TNF induced NF-kappaB and JNK activation in hepatocytes
Ohori et al., Auris Nasus Larynx 2007 (Nasal Polyps) : TNF-alpha upregulates VCAM-1 and NF-kappaB in fibroblasts from nasal polyps ... The activation of NF-kappaB induced by TNF-alpha was determined by electrophoretic mobility shift assays and the influence on the expression of VCAM-1 was investigated ... NF-kappaB activity was enhanced by TNF-alpha stimulation and remarkably suppressed by NF-kappaB proteasome inhibitor
Séguin et al., Matrix Biol 2006 (Disease Models, Animal...) : We demonstrate that in nucleus pulposus cells, the p38 and JNK pathways regulate induction of MMP-1 and -3 ; p38, JNK, and NF-kappaB regulate the induction of MMP-13 ; and ERK regulates the up-regulation of MT1-MMP mRNA in response to TNFalpha
Sappington et al., Invest Ophthalmol Vis Sci 2006 : To investigate how hydrostatic pressure influences regulation of interleukin (IL)-6 by retinal glia and whether this regulation is associated with the ubiquitin/proteasome pathway ( UPP ) and activation of the transcription factor nuclear factor (NF)kappaB
Liuwantara et al., Diabetes 2006 : Activation of NF-kappaB by TNF receptor associated factor (TRAF) 2 , TRAF6, NF-kappaB inducing kinase, or protein kinase D, which transduce signals downstream of Toll-like receptors, TNF receptors, and free radicals, respectively, were all potent activators of the A20 promoter ... Activation of NF-kappaB by TNF receptor associated factor (TRAF) 2, TRAF6, NF-kappaB inducing kinase, or protein kinase D, which transduce signals downstream of Toll-like receptors, TNF receptors, and free radicals, respectively, were all potent activators of the A20 promoter ... Activation of NF-kappaB by TNF receptor associated factor (TRAF) 2, TRAF6 , NF-kappaB inducing kinase, or protein kinase D, which transduce signals downstream of Toll-like receptors, TNF receptors, and free radicals, respectively, were all potent activators of the A20 promoter ... Activation of NF-kappaB by TNF receptor associated factor (TRAF) 2, TRAF6, NF-kappaB inducing kinase, or protein kinase D , which transduce signals downstream of Toll-like receptors, TNF receptors, and free radicals, respectively, were all potent activators of the A20 promoter
Choi et al., World J Gastroenterol 2006 : Although all three MAP kinase family members were phosphorylated in response to TNF-alpha, a selective inhibitor of p38 kinase SB203580 only could inhibit both NF-kappaB dependent transcriptional activity and IL-8 and CCL20 production, suggesting a potential link between p38 kinase and NF-kappaB dependent pathways in AGS cells ... Although all three MAP kinase family members were phosphorylated in response to TNF-alpha, a selective inhibitor of p38 kinase SB203580 only could inhibit both NF-kappaB dependent transcriptional activity and IL-8 and CCL20 production, suggesting a potential link between p38 kinase and NF-kappaB dependent pathways in AGS cells
Ryu et al., World J Gastroenterol 2006 (Hepatitis B) : During the initial state of HBV infection, hepatocytes facilitate the activation of NF-kappaB through up regulation of LT-alpha, TRAF2 , and NIK ... During the initial state of HBV infection, hepatocytes facilitate the activation of NF-kappaB through up regulation of LT-alpha , TRAF2, and NIK
Enk et al., J Ethnopharmacol 2007 (Translocation, Genetic) : Differential effect of Rhizoma coptidis and its main alkaloid compound berberine on TNF-alpha induced NFkappaB translocation in human keratinocytes
Park et al., J Biol Chem 2006 (Carcinoma, Hepatocellular...) : Up-regulation of cyclin D1 by HBx is mediated by NF-kappaB2/BCL3 complex through kappaB site of cyclin D1 promoter ... Here we show that HBx up-regulates cyclin D1 and that this process is mediated by the NF-kappaB2 ( p52 ) /BCL-3 complex
Leung et al., Mol Pharmacol 2006 : Eri-B inhibited the NF-kappaB transcriptional activity but not that of cAMP response element binding protein ... It suppressed the transcription of NF-kappaB downstream gene products including cyclooxygenase-2 and inducible nitric-oxide synthase induced by tumor necrosis factor-alpha or lipopolysaccharide in macrophages and hepatocarcinoma cells
Adachi et al., Nihon Ishinkin Gakkai Zasshi 2006 : Dectin-1 increased TLR2 mediated NF-kappaB activation in response to zymosan ... Dectin-1 increased TLR2 mediated NF-kappaB activation in response to zymosan
Mookherjee et al., J Leukoc Biol 2006 (Inflammation) : Consistent with this, the human and bovine peptides suppressed LPS induced translocation of NF-kappaB subunits p50 and p65 into the nucleus of monocytic cells
Paul et al., Pathobiology 2006 (Liver Cirrhosis, Experimental) : Thalidomide downregulates NF-kappaB induced TNF-alpha and activates hepatic stellate cells (HSC) via inhibition of IkappaB degradation to prevent liver cirrhosis
Chen et al., Assay Drug Dev Technol 2006 : Compound 1 did not inhibit the RANKL induced activation of a NF-kappaB reporter gene, or p38 kinase activity, suggesting a mechanism of action downstream of NF-kappaB
Yammani et al., Arthritis Rheum 2006 : Stimulation of chondrocytes with S100A4 increased the phosphorylation of Pyk-2, MAP kinases, and activated NF-kappaB , followed by increased production of MMP-13 in the conditioned medium
Shaneyfelt et al., Virology journal 2006 : Two of these four compounds, alpha-mangostin and 18-beta-glycyrrhetinic acid, activated NFkappaB and induced IL-8 secretion
Jiang et al., Dev Comp Immunol 2007 : Furthermore, EMSA showed that NF-kappaB activity was induced in abalone hemocytes by stimulation with LPS
Pan et al., Biochem Pharmacol 2006 (Cocarcinogenesis...) : Taken together, these results show that acacetin down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NF kappa B by interfering with the activation PI3K/Akt/IKK and MAPK, suggesting that acacetin is a functionally novel agent capable of preventing inflammation associated tumorigenesis ... Taken together, these results show that acacetin down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NF kappa B by interfering with the activation PI3K/Akt/IKK and MAPK, suggesting that acacetin is a functionally novel agent capable of preventing inflammation associated tumorigenesis
Lee et al., Cytokine 2006 (Tooth Migration) : In addition, dexamethasone inhibited TNF-alpha induced phosphorylation of p38 MAP kinase and extracellular regulated kinases ( ERKs ), IkappaB degradation, and NF-kappaB activation
Kuboki et al., Am J Physiol Gastrointest Liver Physiol 2007 (Hypothermia...) : Furthermore, it appears that Pin1 promotes NF-kappaB p65 protein stability such that decreased expression of Pin1 during ischemia-reperfusion results in p65 degradation, reduced nuclear translocation of NF-kappaB , and enhanced hepatocellular injury
Hwang et al., Carcinogenesis 2007 : Co-treatment of mouse skin with the IKKbeta-specific inhibitor SC-514 ( 1 micromol ) attenuated TPA induced activation of Akt and NF-kappaB , and also the expression of COX-2 in hairless mouse skin ... Taken together, 9Z,11E-CLA inhibits NF-kappaB driven-COX-2 expression by blocking the IKK and PI3K-Akt signaling in TPA treated hairless mouse skin in vivo, which may account for its previously reported anti-tumor promoting effects ... Taken together, 9Z,11E-CLA inhibits NF-kappaB driven-COX-2 expression by blocking the IKK and PI3K-Akt signaling in TPA treated hairless mouse skin in vivo, which may account for its previously reported anti-tumor promoting effects ... Taken together, 9Z,11E-CLA inhibits NF-kappaB driven-COX-2 expression by blocking the IKK and PI3K-Akt signaling in TPA treated hairless mouse skin in vivo, which may account for its previously reported anti-tumor promoting effects
Mann et al., Cancer Res 2006 (Breast Neoplasms) : Our results further indicated that constitutive NF-kappaB reporter activity in pancreatic cancer cells is not affected by dominant negative I kappaB alpha
Li et al., Cancer Res 2006 (Multiple Myeloma) : The secretion of IL-6 by BMSCs was completely inhibited by 10 ( -9 ) mol/L PACAP, which also attenuated the phosphorylation of both p42/44 and p38 mitogen activated protein kinases ( MAPK ) as well as nuclear factor-kappaB (NF-kappaB) activation in response to the adhesion of multiple myeloma cells to BMSCs, whereas the inhibition of p42/44 MAPK signaling attenuated PACAP action ... The secretion of IL-6 by BMSCs was completely inhibited by 10 ( -9 ) mol/L PACAP, which also attenuated the phosphorylation of both p42/44 and p38 mitogen activated protein kinases ( MAPK ) as well as nuclear factor-kappaB (NF-kappaB) activation in response to the adhesion of multiple myeloma cells to BMSCs, whereas the inhibition of p42/44 MAPK signaling attenuated PACAP action ... The secretion of IL-6 by BMSCs was completely inhibited by 10 ( -9 ) mol/L PACAP, which also attenuated the phosphorylation of both p42/44 and p38 mitogen activated protein kinases ( MAPK ) as well as nuclear factor-kappaB (NF-kappaB) activation in response to the adhesion of multiple myeloma cells to BMSCs, whereas the inhibition of p42/44 MAPK signaling attenuated PACAP action
Brown et al., Ann Clin Lab Sci 2006 (Neoplasms, Squamous Cell...) : Because the mammalian target of the rapamycin (mTOR) pathway contributes to the activation of NF-kappaB through immunophilin/mTOR signaling , we investigated : ( a ) the immunohistochemical expression and state of activation and potential clinical significance of components of the mTOR signal transduction pathway in SCCT patients ( morphoproteomics ) ; and ( b ) the inhibitory effects of rapamycin on the growth and state of activation of mTOR in 2 HNSCC cell lines ( pharmacoproteomics )
Alvarez et al., J Immunol 2006 (MAP Kinase Signaling System) : When analyzing the role of these pathways on neutrophil functions, we observed that up-regulation of CD11b triggered by bacterial DNA was decreased by pharmacological inhibitors of the p38 MAPK, ERK1/2, and JNK, whereas stimulation of IL-8 release was dependent on p38, ERK1/2, and NF-kappaB
Papavlassopoulos et al., J Immunol 2006 (Inflammation) : We present data supporting that proximal regulation of the inhibitory factor IkappaB-alpha is critically involved in the observed inhibition of NF-kappaB translocation
Jackson-Bernitsas et al., Oncogene 2007 (Carcinoma, Squamous Cell...) : The expression of constitutively active NF-kappaB in HNSCC is mediated through the tumor necrosis factor (TNF) signaling pathway, as NF-kappaB reporter activity was inhibited by DN-TNF receptor associated death domain ( TRADD ), DN-TNF receptor associated factor (TRAF)2, DN-receptor interacting protein ( RIP ), DN-transforming growth factor-beta activated kinase 1 ( TAK1 ), DN-kappa-Ras, DN-AKT and DN-IKK but not by DN-TRAF5 or DN-TRAF6 ... The expression of constitutively active NF-kappaB in HNSCC is mediated through the tumor necrosis factor (TNF) signaling pathway, as NF-kappaB reporter activity was inhibited by DN-TNF receptor associated death domain ( TRADD ), DN-TNF receptor associated factor (TRAF)2, DN-receptor interacting protein ( RIP ), DN-transforming growth factor-beta activated kinase 1 ( TAK1 ), DN-kappa-Ras, DN-AKT and DN-IKK but not by DN-TRAF5 or DN-TRAF6 ... The expression of constitutively active NF-kappaB in HNSCC is mediated through the tumor necrosis factor (TNF) signaling pathway, as NF-kappaB reporter activity was inhibited by DN-TNF receptor associated death domain ( TRADD ), DN-TNF receptor associated factor (TRAF)2, DN-receptor interacting protein ( RIP ), DN-transforming growth factor-beta activated kinase 1 ( TAK1 ), DN-kappa-Ras, DN-AKT and DN-IKK but not by DN-TRAF5 or DN-TRAF6
Kikuchi et al., Oncogene 2006 : Ectopic expression of NF-kappaB decreased a half-life of p73alpha by increasing its ubiquitination levels, and thereby inhibiting the transcriptional activity as well as proapoptotic function of p73alpha, whereas NF-kappaB had undetectable effects on p53
Ou et al., Biochem J 2007 : Overexpression of p50 and p65 decreased BHMT promoter activity, while blocking NF-kappaB activation increased BHMT expression and promoter activity, and prevented SAM but not MTA 's ability to inhibit BHMT expression
Dunsmore et al., Inflamm Res 2006 : After a 4 h recovery period from heat shock, there was inhibition of tumor necrosis factor-alpha mediated NF-kappaB activation
Markovic et al., J Mol Med (Berl) 2006 (Arthritis, Rheumatoid) : As to the mechanism of inhibition, electrophoretic mobility shift assay experiments demonstrated that exposure of FLS to hyperthermia prevents IL-1beta induced NF-kappaB translocation and subsequent DNA binding
Ramana et al., J Biol Chem 2006 : The AR inhibition or ablation significantly attenuated LPS induced activation of protein kinase C ( PKC ) and phospholipase C (PLC), nuclear translocation of NF-kappaB , and phosphorylation and proteolytic degradation of IkappaBalpha in macrophages
Zbytek et al., Peptides 2006 : CRH inhibits NF-kappa B signaling in human melanocytes ... CRH inhibits NF-kappaB activity in human immortalized epidermal ( PIG1 ) melanocytes
Ghosh et al., Cancer Cell 2006 (MAP Kinase Signaling System) : Furthermore, in TSC2 ( -/- ) MEFs, the rapamycin mediated inhibition of deregulated mTOR activity restored NF-kappaB activation and survival
Cui et al., Circ Res 2006 : In vascular smooth muscle cells ( VSMCs ), Ang II signaling activates interleukin (IL)-6 expression, a cytokine producing acute-phase inflammation, mediated by the transcription factor nuclear factor kappaB (NF-kappaB)
Kuo et al., Cancer Lett 2007 (Breast Neoplasms) : Furthermore, rugosin E also inhibited the TNF-alpha activated NF-kappaB dependent reporter gene expression of cyclin D1 , c-Myc, XIAP, Bcl-2, and Bcl-X ( L ) were all downregulated by rugosin E
Shiraki et al., Int J Mol Med 2006 (Hepatitis C, Chronic...) : The effect of CD40 stimulation on NF-kappaB activation was determined in NF-kappaB reporter gene transfected HepG2 cells with the Luciferase assay
Ji et al., Zhong Xi Yi Jie He Xue Bao 2006 : HXR could down-regulate the expression of membrane receptor TLR4 and inhibit the expressions of NF-kappaB and TNF-alpha ... QGHXR could down regulate the expressions of membrane receptors CD14 and TLR(4) and inhibit the expressions of NF-kappaB and TNF-alpha ... QGHXR could down regulate the expressions of membrane receptors CD14 and TLR(4) and inhibit the expressions of NF-kappaB and TNF-alpha ... QGHXR can protect liver cells by down regulating the expressions of CD14, TLR(4) and NF-kappaB and inhibiting TNF-alpha expression
Borrás et al., FASEB J 2006 : Phosphorylation of extracellular regulated kinase (ERK)1/2 led to an activation of NFkappaB , as indicated by increased p50 subunit expression in nuclear extracts, and increased mRNA levels of the antioxidant enzyme manganese-superoxide dismutase ( MnSOD ) ... Phosphorylation of extracellular regulated kinase (ERK)1/2 led to an activation of NFkappaB , as indicated by increased p50 subunit expression in nuclear extracts, and increased mRNA levels of the antioxidant enzyme manganese-superoxide dismutase ( MnSOD ) ... Phosphorylation of extracellular regulated kinase (ERK)1/2 led to an activation of NFkappaB , as indicated by increased p50 subunit expression in nuclear extracts, and increased mRNA levels of the antioxidant enzyme manganese-superoxide dismutase ( MnSOD )
Kawadler et al., Cancer Biol Ther 2006 : The mechanism by which Lys63 linked polyubiquitin chains participate in signaling cascades has yet to be determined, but two recent publications ( Wu et al., Nat Cell Bio 2006 ; 8 : 398-406 and Ea et al., Mol Cell 2006 ; 22 : 245-57 ) shed light on how this distinctive modification functions in NFkappaB activation by TNFalpha ... The polyubiquitin chain on RIP1 is recognized and bound by NEMO, the regulatory subunit of the IKK complex, and this binding is essential for NFkappaB activation by TNFalpha
Xie et al., Zhongguo Zhong Xi Yi Jie He Za Zhi 2006 (Asthma) : Herba Epimedii and Radix Astragali could effectively reduce the production of TNF-alpha and inhibit NF-kappa B activity, and the efficacies in the three treated group were similar, showing insignificant difference among them ... Application of Herba Epimedii and Radix Astragali in the attack or remission stage of asthma could restrain the development of inflammation by reducing the production of TNF-alpha and inhibiting NF-kappa B activity
Li et al., Regul Pept 2007 (Liver Cirrhosis, Experimental) : However, the signal transduction mechanism underlying effects of Angiotensin II (Ang II) and Aldosterone ( Aldo ) on Nuclear Factor-kappaB (NF-kappaB) and active protein-1 (AP-1) pathway in hepatic fibrogenesis remains to be fully elucidated ... The present study aims to investigate the signal transduction mechanism underlying effects of Ang II and Aldo on NF-kappaB and AP-1 pathway during hepatic fibrogenesis
Ohnishi et al., Radiat Res 2006 (Carcinoma, Non-Small-Cell Lung) : In addition, radiation induced expression of the transcription factor NF-kappaB (NFKB) and XIAP ( X-chromosome linked inhibitor of apoptosis protein ) was suppressed by NBS1-siRNA
Csiszar et al., Am J Physiol Heart Circ Physiol 2006 : Thus resveratrol at nutritionally relevant concentrations inhibits TNF-alpha induced NF-kappaB activation and inflammatory gene expression and attenuates monocyte adhesiveness to HCAECs
Hakala et al., Arterioscler Thromb Vasc Biol 2006 (Inflammation) : H-LDL induced rapid phosphorylation of the p38 mitogen activated protein kinase ( MAPK ), nuclear translocation of 2 transcription factors, nuclear factor kappaB (NF-kappaB) and activator protein 1 (AP-1), and time dependent secretion of IL-8 from the macrophages
Suzuki et al., Biomed Pharmacother 2006 : DHMEQ inhibited lipopolysaccharide (LPS) induced NF-kappaB activation by inhibiting its nuclear translocation from the cytoplasm
Vilela et al., Int Immunopharmacol 2006 : As fenretinide is a ceramide up-regulating drug that inhibits the activation of the pro-inflammatory transcriptional factor, nuclear factor (NF)-kappaB, the impact of fenretinide on unstimulated and tumor necrosis factor (TNF)-alpha stimulated production of NF-kappaB dependent interleukin (IL)-8 was studied in immortalized wild-type ( non-CF ; 9HTEo- ) and mutant DeltaF508 CFTR ( CF ; CFTE29o- ) tracheal epithelial cells
Takenaka et al., J Mol Cell Cardiol 2006 (Heart Failure...) : Local activation of Ang-II in hypertrophic LV triggers MMP mediated ECM degradation, namely LV remodeling, at least in part, through NADPH oxidase induced oxidative stress and the subsequent NFkappaB activation
Abid et al., J Biol Chem 2006 : VEGF induction of the forkhead dependent genes was down-regulated by the NF-kappaB inhibitor, constitutively active Ad-IkappaB, and in some cases by the nuclear factor of activated T-cells ( NF-AT ) inhibitor, cyclosporin
Butler et al., J Biol Chem 2006 : Elafin prevents lipopolysaccharide induced AP-1 and NF-kappaB activation via an effect on the ubiquitin-proteasome pathway ... In this report we have shown that elafin inhibits the lipopolysaccharide induced production of monocyte chemoattractant protein-1 in monocytes by inhibiting AP-1 and NF-kappaB activation
Pick et al., Biochem Biophys Res Commun 2006 : The functional consequences of the identified PIDD/nucleolin interaction remain to be elucidated, but may be related to a recently discovered new role for PIDD in the activation of NF-kappaB upon genotoxic stress
Horvath et al., Exp Hematol 2006 (Leukemia, Promyelocytic, Acute) : The apoptotic potential of M8 and RV was compared using a specific double staining method and inhibition of TNF-alpha induced activation of NF-kappaB was studied
Ma et al., Nan Fang Yi Ke Da Xue Xue Bao 2006 : To investigate nuclear factor kappa B (NF-kappaB) activation induced by lipopolysaccharide (LPS) in rat peritoneal macrophages ( PMAs ) and the inhibitory effect of resveratrol on NF-kappaB activation ... Resveratrol can inhibit LPS induced NF-kappaB activation in rat PMAs and subsequently suppress the expressions of TNF-alpha, IL-1 and NO
Hunter et al., J Biol Chem 2006 : TNF-alpha engagement of TNFR1 recruited the adaptor proteins TRADD, TRAF-2, and RIP into lipid rafts and activated RhoA, NF-kappaB , and MAPK pathways ... TNF-alpha engagement of TNFR1 recruited the adaptor proteins TRADD, TRAF-2, and RIP into lipid rafts and activated RhoA, NF-kappaB , and MAPK pathways ... By contrast, TNF-alpha induced activation of NF-kappaB and MAPKs was unaffected by methyl-beta-cyclodextrin indicating that, in airway smooth muscle cells, activation of these pathways occurred independently of lipid rafts
Ding et al., J Biol Chem 2006 : Overexpression of IKKbeta-KM , a kinase inactive mutant of IKKbeta, blocked NF-kappaB activation and COX-2 induction by nickel compounds, indicating that activated NF-kappaB may be a mediator for COX-2 induction ... Overexpression of IKKbeta-KM, a kinase inactive mutant of IKKbeta, blocked NF-kappaB activation and COX-2 induction by nickel compounds, indicating that activated NF-kappaB may be a mediator for COX-2 induction
Paland et al., Cell Microbiol 2006 : Cpn induced the activation of nuclear factor kappa B (NF-kappaB) and inhibition of NF-kappaB with a chemical inhibitor or by silencing expression of the p65 subunit sensitized infected cells for apoptosis induction by staurosporine or TNFalpha
Khanbolooki et al., Mol Cancer Ther 2006 (Pancreatic Neoplasms) : NF-kappaB inhibition resulted in down-regulation of BCL-XL and XIAP, and silencing either restored TRAIL sensitivity in TRAIL-resistant cells ... NF-kappaB inhibition resulted in down-regulation of BCL-XL and XIAP , and silencing either restored TRAIL sensitivity in TRAIL-resistant cells
Han et al., American journal of physiology. Renal physiology 2007 : Thus the inhibition of [ ( 3 ) H ] thymidine incorporation caused by uric acid can be explained by two distinct mechanisms, the activation of NF-kappaB as well as the activation of PLA(2)
Hirai et al., Osaka City Med J 2006 : AP-1 DNA binding activities in the FW and SW of group R significantly increased at POD 5, and NF-kappaB DNA binding activities of group R significantly increased at PODs 3 and 5
Jo et al., Antioxid Redox Signal 2006 (Atherosclerosis...) : The NADPH oxidase activation by BMP4 then leads to ROS production, NF-kappaB activation, intercellular adhesion molecule 1 ( ICAM-1 ) expression, and subsequent increased monocyte adhesivity of endothelial cells ... The NADPH oxidase activation by BMP4 then leads to ROS production, NF-kappaB activation, intercellular adhesion molecule 1 ( ICAM-1 ) expression, and subsequent increased monocyte adhesivity of endothelial cells
Widera et al., BMC neuroscience 2006 : Furthermore, TNF strongly activated nuclear factor-kappa B (NF-kappaB) as measured by reporter gene assays and by an activity-specific antibody ... TNF mediated activation of IKK-beta resulted in activation of NF-kappaB and was followed by up-regulation of the bona-fide target gene cyclin D1 ... TNF mediated activation of IKK-beta resulted in activation of NF-kappaB and was followed by up-regulation of the bona-fide target gene cyclin D1
Park et al., J Biol Chem 2006 : Recently we reported that transglutaminase 2 (TGase 2) activates nuclear factor-kappaB (NF-kappaB) independently of I-kappaB kinase (IKK) activation, by inducing cross linking and protein polymer formation of inhibitor of nuclear factor-kappaBalpha ( I-kappaBalpha )
Groesdonk et al., Infect Immun 2006 : This is associated with reduced expression of NF-kappaB dependent antiapoptotic c-IAP-2 and a loss of the mitochondrial transmembrane potential
Park et al., Life Sci 2006 : Herein we examine the effects of a newly identified synthetic PPARgamma ligand, ascochlorin-6 ( AS-6 ), on TNF-alpha stimulated NF-kappaB activity and inflammatory molecule expression in vascular smooth muscle cells ( VSMCs ) ... AS-6 successfully inhibited TNF-alpha stimulated NF-kappaB activity and inflammatory molecule expression, including vascular cell adhesion molecule-1 ( VCAM-1 ), monocyte chemotactic protein-1 (MCP-1), and fractalkine ( CX3CL1 ) ... Transient transfection with an [ NF-kappaB ] x4 luciferase reporter construct showed that AS-6 inhibition of TNF-alpha stimulated NF-kappaB activation was PPARgamma dependent ... The inhibitory effects of AS-6 on TNF-alpha stimulated inflammatory gene expression and NF-kappaB activation were more potent than those of rosiglitazone and pioglitazone
Sharma et al., Toxicology 2006 (Mouth Neoplasms...) : STE induced nuclear translocation and DNA binding activity of NF-kappaB were inhibited in curcumin pretreated oral premalignant and cancer cells in vitro
Ni et al., Oral Oncol 2007 (Oral Submucous Fibrosis) : NF-kappaB expression induced by safrole in fibroblasts may be mediated by ERK activation and COX-2 signal transduction pathway
Contzler et al., Biochem Biophys Res Commun 2006 : Histone acetyltransferase HBO1 inhibits NF-kappaB activity by coactivator sequestration ... Since the androgen receptor and NF-kappaB transcription factors crossmodulate their transcriptional activity, we investigated whether HBO1 regulates NF-kappaB signaling ... Here, we report that in 293T cells HBO1 reduced dose-dependently NF-kappaB activity stimulated by TNFalpha, or by overexpressing p65/RelA, RelB, or cRel ... Electrophoretic mobility-shift assays demonstrated that HBO1 was neither perturbing the formation of p65/RelA DNA complexes nor binding itself to the kappaB consensus sequence or to p65/RelA, suggesting that HBO1 reduced NF-kappaB activity by squelching a cofactor
Mishra et al., Int J Cancer 2006 (Carcinoma, Squamous Cell...) : The NF-kappaB DNA binding is primarily due to homodimerization of p50 but infection of high risk HPV promotes participation of p65 in NF-kappaB complex formation, leading to heterodimerization of p50/p65
Wang et al., Oncogene 2007 (Neoplasms) : Using RNA interference, we demonstrated that the chemotherapy induced HB-EGF was largely dependent on activator protein-1 (AP-1) and NF-kappaB activation
Liu et al., Oncogene 2007 (Melanoma) : Oncogenic BRAF regulates beta-Trcp expression and NF-kappaB activity in human melanoma cells ... Taken together, these data support a model in which mutational activation of BRAF in human melanomas contributes to constitutive induction of NF-kappaB activity and to increased survival of melanoma cells
Biswas et al., J Cell Physiol 2006 (Breast Neoplasms) : This article is focused on the role of NF-kappaB activation initiated by the EGFR family receptors in subclasses of breast cancer
Bamford et al., Cell Microbiol 2007 (Burkholderia Infections) : Highly purified lipopolysaccharides from Burkholderia cepacia complex clinical isolates induce inflammatory cytokine responses via TLR4 mediated MAPK signalling pathways and activation of NFkappaB
Andrassy et al., Am J Pathol 2006 (Disease Models, Animal...) : Inflamed gut biopsy tissue demonstrated a significant up-regulation of RAGE and increased NF-kappaB activation
Wang et al., Cell Signal 2007 : Polyglutamine expanded ataxin-7 decreases nuclear translocation of NF-kappaB p65 and impairs NF-kappaB activity by inhibiting proteasome activity of cerebellar neurons ... Proteasome degradation of IkappaBalpha permits nuclear translocation of NF-kappaB and is required for continuous NF-kappaB activity, which supports the survival of cultured cerebellar neurons by inducing Bcl-x ( L ) expression ... Thus, we tested the hypothesis that mutant ataxin-7-Q75 causes proteasome dysfunction and impairs NF-kappaB activity, leading to reduced Bcl-x ( L ) expression, caspase activation and cerebellar neuronal death
Yao et al., Pharmacol Res 2006 (Carcinoma...) : Coincident with this observation, TSA induced a concentration dependent reduction of constitutive and tumour necrosis factor (TNF)-alpha induced NF-kappaB activation in Tca8113 cells ... Coincident with this observation, TSA induced a concentration dependent reduction of constitutive and tumour necrosis factor (TNF)-alpha induced NF-kappaB activation in Tca8113 cells ... Overall, our results indicate inhibition of NF-kappaB activation contributes , at least partially, to the antitumour activity of TSA in human tongue carcinoma cells
Yamamoto-Furusho et al., J Biol Chem 2006 : Functionally, CENTB1 overexpression inhibited NOD1- and NOD2 dependent activation of NF-kappaB , whereas small inhibitory RNA against CENTB1 increased NF-kappaB activation following NOD1- or NOD2 mediated recognition of the bacterial components gamma-d-glutamyl-meso-diaminopimelic acid and muramyl dipeptide, respectively ... Functionally, CENTB1 overexpression inhibited NOD1- and NOD2 dependent activation of NF-kappaB , whereas small inhibitory RNA against CENTB1 increased NF-kappaB activation following NOD1- or NOD2 mediated recognition of the bacterial components gamma-d-glutamyl-meso-diaminopimelic acid and muramyl dipeptide, respectively ... Functionally, CENTB1 overexpression inhibited NOD1- and NOD2 dependent activation of NF-kappaB , whereas small inhibitory RNA against CENTB1 increased NF-kappaB activation following NOD1- or NOD2 mediated recognition of the bacterial components gamma-d-glutamyl-meso-diaminopimelic acid and muramyl dipeptide, respectively ... In contrast, CENTB1 had no effect on NF-kappaB activation induced by Toll-like receptors
Montag et al., J Virol 2006 : Consistent with this, we further show that an HCMV variant that has lost the ability to downregulate TNF-alpha induced NF-kappaB signaling also fails to downregulate surface expression of TNF receptor 1, thereby mechanistically linking the inhibition of TNF-alpha induced NF-kappaB signaling by HCMV to TNF receptor targeting
Banzi et al., Biochem Biophys Res Commun 2006 : Polycystin-1 promotes PKCalpha mediated NF-kappaB activation in kidney cells ... Polycystin-1 promotes PKCalpha mediated NF-kappaB activation in kidney cells ... CTT dependent NF-kappaB promoter activation was mediated by PKCalpha because it was blocked by its specific inhibitor Ro-320432 ... These data reveal, therefore, a novel pathway by which polycystin-1 activates a PKCalpha mediated NF-kappaB signalling and cell survival
Zhang et al., Endocrinology 2007 : To further understand the mechanism of TNF stimulated growth of primary MEC, the requirement for NFkappaB1/p50 , and the role of cyclin D1 in TNF stimulated growth were examined ... However, TNF induced a p52/RelB NFkappaB DNA binding complex in p50 null MEC nuclear extracts
Schopf et al., Arthritis Rheum 2006 (Arthritis, Experimental...) : The IKK complex regulates NF-kappaB activation, an important pathway implicated in the rheumatoid arthritis ( RA ) disease process
Moriyama et al., Biochem Biophys Res Commun 2006 : ANP inhibits LPS induced stimulation of rat microglial cells by suppressing NF-kappaB and AP-1 activations ... NF-kappaB and AP-1 activities were enhanced in LPS stimulated microglial cells, and these enhancements were significantly suppressed by ANP ( 10 ( -6 ) M )
Grandjean-Laquerriere et al., Biomaterials 2007 (Inflammation) : We investigated the production of TNF-alpha and the activation of the nuclear transcription factor NF-kappaB
Koul et al., Biochem Biophys Res Commun 2006 (Glioma) : We found that PTEN inhibited NFkappaB activation induced by TNF ... We found that PTEN inhibited NFkappaB activation induced by TNF
Vogel et al., Biochim Biophys Acta 2006 : In transient transfection reporter gene assays, SSAT2 functions as a transcriptional coactivator for NF-kappaB and cooperates with CBP and P/CAF to enhance TNFalpha induced NF-kappaB activity
Scata et al., Cancer Biol Ther 2006 (Neoplasms) : ATM then is carried into the cytoplasm with NEMO allowing for the activation of IKK and thus NF-kappaB activation
Das et al., Free radical research 2006 (Myocardial Infarction...) : Cardioprotective effect of resveratrol via HO-1 expression involves p38 map kinase and PI-3-kinase signaling, but does not involve NFkappaB ... Cardioprotective effect of resveratrol via HO-1 expression involves p38 map kinase and PI-3-kinase signaling, but does not involve NFkappaB
He et al., J Exp Med 2006 : 25 : 280-288 ). We demonstrate that loss of tumor necrosis factor (TNF) receptor associated factor 3 ( TRAF3 ) results in constitutive noncanonical NF-kappaB activity ... 25 : 280-288 ). We demonstrate that loss of tumor necrosis factor (TNF) receptor associated factor 3 ( TRAF3 ) results in constitutive noncanonical NF-kappaB activity
Yan et al., J Immunol 2006 (Inflammation) : IL-1beta activated NF-kappaB but not ERK or p38
Nair et al., J Immunol 2006 (Neoplasm Invasiveness) : The suppression of TNF induced NF-kappaB activation by deguelin occurred through the inhibition of the activation of IkappaBalpha kinase, leading to sequential suppression of IkappaBalpha phosphorylation, IkappaBalpha degradation, p65 phosphorylation, p65 nuclear translocation, and NF-kappaB dependent reporter gene expression ... Deguelin also suppressed the NF-kappaB reporter activity induced by TNFR1 , TNFR associated death domain, TNFR associated factor 2, and IkappaBalpha kinase, but not that induced by p65 ... Deguelin also suppressed the NF-kappaB reporter activity induced by TNFR1, TNFR associated death domain, TNFR associated factor 2, and IkappaBalpha kinase, but not that induced by p65 ... Deguelin also suppressed the NF-kappaB reporter activity induced by TNFR1, TNFR associated death domain, TNFR associated factor 2, and IkappaBalpha kinase, but not that induced by p65
H-Zadeh et al., Int J Oncol 2006 (Carcinoma...) : Although not associated with changes in Bcl-2, Bcl-XL, Bax, Bad or Bak expression levels, we report that the expression of the pro-apoptotic 1-95IGFBP-3 fragment is associated with the inhibition of TNFalpha induced NF-kappaB activity, similar to that reported for the full length IGFBP-3 protein
Tran et al., Cancer Res 2006 (Brain Neoplasms...) : Activation of Fn14 signaling by the ligand TNF-like weak inducer of apoptosis ( TWEAK ) stimulates migration and up-regulates expression of Fn14 ; this TWEAK effect requires Rac1 and nuclear factor-kappaB (NF-kappaB) activity
Sury et al., Free Radic Biol Med 2006 (MAP Kinase Signaling System) : Intriguingly, NAC had no effect on the initial activation ( i.e., IkappaB degradation, nuclear p65 translocation, and Ser536 phosphorylation ) of NF-kappaB by TNF-alpha ... Intriguingly, NAC had no effect on the initial activation ( i.e., IkappaB degradation, nuclear p65 translocation, and Ser536 phosphorylation ) of NF-kappaB by TNF-alpha
Yan et al., Am J Physiol Gastrointest Liver Physiol 2007 (Colitis) : Finally, we demonstrated that a reduction of Sp1 or NF-kappaB expression reduced CD98 protein expression in unstimulated and IFN-gamma stimulated Caco2-BBE cells
Martucci et al., J Leukoc Biol 2007 : The transcription factor NF-kappaB is a central regulator of immunity, and of the NF-kappaB family, RelB is particularly involved in the expression of genes important in immune responses
Fernandes et al., Exp Eye Res 2006 : Consequences of an impairment of the UPP include accumulation of HIF-1alpha and diminished NF-kappaB activation , which lead to enhanced expression and secretion of pro-angiogenic factors and attenuated expression of MCP-1
Skayian et al., Life Sci 2006 : It was concluded that TNF-alpha activates NF-kappaB and AP-1 and induces PGE ( 2 ) release which alters dose-dependently the activity of the pump by activating different EP receptors with different affinities for PGE ( 2 )
Ahn et al., Mol Pharmacol 2007 (Adenocarcinoma...) : We found that embelin inhibited tumor necrosis factor (TNF) alpha induced NF-kappaB activation
Komine et al., Mol Immunol 2007 (Periodontitis) : Con A low-affinity saliva Lf peptides, PR3 treated Lf preparation and two of four synthetic polypeptides induced the production of interleukin IL-6, monocyte chemoattractant protein-1 and IL-8, and the activation of NF-kappaB in human oral epithelial HSC-2 cells ... Con A low-affinity saliva Lf peptides, PR3 treated Lf preparation and two of four synthetic polypeptides induced the production of interleukin IL-6, monocyte chemoattractant protein-1 and IL-8, and the activation of NF-kappaB in human oral epithelial HSC-2 cells
Kim et al., Amino Acids 2008 (Stomach Neoplasms) : Both Ku70 and Ku80 expressions are mediated by constitutively activated NF-kappaB and constitutively expressed cyclooxygenase-2 in gastric cancer cells, which may be related to gastric cell proliferation and carcinogenesis ... Both Ku70 and Ku80 expressions are mediated by constitutively activated NF-kappaB and constitutively expressed cyclooxygenase-2 in gastric cancer cells, which may be related to gastric cell proliferation and carcinogenesis
Broad et al., Immunology 2007 : In this study we have shown that, whilst NF-kappaB activation and production of TNF-alpha and interleukin-12 by murine RAW264.7 and J774.2 cells in response to stimulation by TLR4, -5, -7 or -9, was reduced by prior stimulation with TLR4 , -5, -7 or -9 ligands, the primary stimulation of TLR3, which does not use the MyD88 pathway, did not reduce the TNF-alpha or interleukin-12 responses to subsequent TLR stimulation ... In this study we have shown that, whilst NF-kappaB activation and production of TNF-alpha and interleukin-12 by murine RAW264.7 and J774.2 cells in response to stimulation by TLR4, -5, -7 or -9, was reduced by prior stimulation with TLR4, -5, -7 or -9 ligands, the primary stimulation of TLR3 , which does not use the MyD88 pathway, did not reduce the TNF-alpha or interleukin-12 responses to subsequent TLR stimulation
Chang et al., J Biol Chem 2006 (Inflammation...) : An earlier study found that IL-17 activates NF-kappaB and MAPK pathways and requires TRAF6 to induce IL-6 ... An earlier study found that IL-17 activates NF-kappaB and MAPK pathways and requires TRAF6 to induce IL-6
Law et al., J Virol 2007 : Our results showed that the SARS coronavirus nsp-1 plays an important role in CCL5, CXCL10, and CCL3 expression in human lung epithelial cells via the activation of NF-kappaB ... Our results showed that the SARS coronavirus nsp-1 plays an important role in CCL5 , CXCL10, and CCL3 expression in human lung epithelial cells via the activation of NF-kappaB ... Our results showed that the SARS coronavirus nsp-1 plays an important role in CCL5, CXCL10 , and CCL3 expression in human lung epithelial cells via the activation of NF-kappaB
Vila-del Sol et al., J Leukoc Biol 2007 : The role of NF-kappaB activation in iNOS induction by IFN-gamma was corroborated by overexpression of the NF-kappaB inhibitory protein IkappaBalpha, which inhibited iNOS promoter activity induced by IFN-gamma
Kawakami et al., Arterioscler Thromb Vasc Biol 2007 : ApoCIII further activated nuclear factor-kappaB (NF-kappaB) through PKC alpha in THP-1 cells and augmented beta1-integrin expression
Zhu et al., J Clin Invest 2006 : We further demonstrated that NF-kappaB2 was required for thymic Aire gene transcriptional regulation
Hernández-Vargas et al., Breast Cancer Res Treat 2007 (Breast Neoplasms) : NF-kappaB transcriptional activity in MDA-MB-231 cells was dependent on IkappaB-alpha phosphorylation, as shown by functional experiments using the specific inhibitor BAY11-7082
Xu et al., J Laryngol Otol 2007 (Chronic Disease...) : The present study investigated the role of nuclear factor-kappa B (NF-kappaB) activation in upregulating the expression of interleukin-5 , -6 and -8 ( IL-5, IL-6 and IL-8 )
Williams et al., Am J Physiol Lung Cell Mol Physiol 2007 : Expression of inhibitory peptides and constitutively active G protein mutants revealed that G ( q ) signaling was both necessary for Tacr1 induced NF-kappaB activation and sufficient for NF-kappaB activation in the absence of any other treatment ... Treatment with pharmacological inhibitors to investigate events downstream of G ( q ) revealed that Tacr1 induced NF-kappaB activation proceeded through an intracellular signaling pathway that was dependent on phospholipase C, calcium, Ras, Raf-1, MEK, Erk, and proteasome function
Yang et al., Am J Physiol Lung Cell Mol Physiol 2007 (Inflammation) : We hypothesized that cigarette smoke mediated proinflammatory cytokine release is regulated by SIRT1 by its interaction with NF-kappaB in a monocyte-macrophage cell line ( MonoMac6 ) and in inflammatory cells of rat lungs
Shiah et al., Mol Cancer Ther 2006 (Pancreatic Neoplasms) : The inhibition of NF-kappaB activity is dependent on the down-regulation of nuclear phosphorylated p65 , a component of the active form of the NF-kappaB complex
Taylor et al., J Virol 2006 (Cytomegalovirus Infections) : Furthermore, IE86 expression inhibits tumor necrosis factor alpha induced NFkappaB DNA binding and target gene expression
Syed et al., Apoptosis 2006 : In this report, we show that constitutive high-level expression of UEV1A alone in cultured human cells was sufficient to cause a significant increase in NF-kappaB activity as well as the expression of its target anti-apoptotic protein, Bcl-2 ( B-cell leukemia/lymphoma 2 )
Dai et al., Free Radic Biol Med 2006 (Atherosclerosis...) : Furthermore, overexpressed Ref-1 upregulated NF-kappaB and MCP-1 promoter activity, and antisense Ref-1 reduced Hcy induced NF-kappaB DNA binding activity and MCP-1 secretion ... These data indicate that Hcy induced ROS upregulate the expression and translocation of Ref-1 via NADPH oxidase, and then Ref-1 increases NF-kappaB activity and MCP-1 secretion in human monocytes/macrophages, which may accelerate the development of atherosclerosis
Suzuki et al., Trends Immunol 2006 : Although each system seems to possess distinct activation mechanisms, interleukin-1 receptor associated kinase ( IRAK)-4 is essential for NF-kappaB activation in Toll-like receptor ( TLR ) and T-cell receptor ( TCR ) signaling pathways ... Although each system seems to possess distinct activation mechanisms, interleukin-1 receptor associated kinase ( IRAK)-4 is essential for NF-kappaB activation in Toll-like receptor ( TLR ) and T-cell receptor ( TCR ) signaling pathways
Ueno et al., J Surg Res 2006 : Lack of Cybb had little effect on NF-kappa B and STAT3 binding, and no effect in TNFa and interleukin-6 production after PH. Cybb KO mice had normal liver structure and similar levels of hepatocyte DNA replication as those of wild type mice
Kinoshita et al., Int Immunol 2006 : When co-expressed in HEK293 cells, FAF1 interfere with the NF-kappaB activation induced by PYPAF1 and ASC ... In THP-1 cells, the stimulation of NF-kappaB up-regulated the level of endogenous FAF1
Rébé et al., Blood 2007 : Caspase-8 prevents sustained activation of NF-kappaB in monocytes undergoing macrophagic differentiation
Häcker et al., Science's STKE : signal transduction knowledge environment 2006 : IKKbeta ( and IKKgamma ) are essential for rapid NF-kappaB activation by proinflammatory signaling cascades, such as those triggered by tumor necrosis factor alpha (TNFalpha) or lipopolysaccharide (LPS)
Pappu et al., Eur J Immunol 2006 : NF-kappaB activation through B cell receptor (BCR) ligation is critical for B cell development, survival and antigen mediated activation of B cells ... CARD domain and MAGUK-domain containing protein-1 ( CARMA1 ), recently identified adaptor molecule, has been shown to play an essential role in BCR induced NF-kappaB activation
Stio et al., J Steroid Biochem Mol Biol 2007 (Crohn Disease) : At the same time, NF-kappaB protein levels present in cytoplasmic extracts decreased in the presence of TNF-alpha and increased when PBMC were incubated with TX 527 ... The results of our studies indicate that TX 527 inhibits TNF-alpha mediated effects on PBMC and the activation of NF-kappaB and that its action is mediated by Vitamin D receptor (VDR), which is activated when the cells are stimulated with TX 527 ... The results of our studies indicate that TX 527 inhibits TNF-alpha mediated effects on PBMC and the activation of NF-kappaB and that its action is mediated by Vitamin D receptor (VDR) , which is activated when the cells are stimulated with TX 527
Chikamatsu et al., Mol Immunol 2007 : Effects of Cot expression on the nuclear translocation of NF-kappaB in RBL-2H3 cells ... This finding gives a new insight into the role of Cot in the FcvarepsilonRI mediated NF-kappaB activation in mast cells
Mendelson et al., J Steroid Biochem Mol Biol 2006 : In this manuscript, we will review findings to suggest that induction of COX-2 by inflammatory cytokines acting through NF-kappaB contributes to the increase in CYP19 expression and breast cancer progression, and that PR plays a dominant protective role in breast cancer cells by antagonizing NF-kappaB activation of COX-2
Brinkmann et al., J Virol 2007 : K15 interacts with cellular proteins, TRAF ( tumor necrosis factor receptor associated factor ) and Src kinases, and activates AP-1, NF-kappaB , and the mitogen activated protein kinases ( MAPKs ) c-jun-N-terminal kinase and extracellular signal regulated kinase
Braeuer et al., Mol Cancer Res 2006 (Neoplasms) : We studied different cell lines displaying varying responses to TRAIL and found that TRAIL can activate NF-kappaB in all our cancer cell lines regardless of their TRAIL sensitivity
Wong et al., Int Immunopharmacol 2006 : However, the expression of VCAM-1 on BEAS-2B cells was only up-regulated by TNF-alpha induced NF-kappaB activity ... However, the expression of VCAM-1 on BEAS-2B cells was only up-regulated by TNF-alpha induced NF-kappaB activity
Lee et al., Biochem Biophys Res Commun 2006 (MAP Kinase Signaling System) : Reporter gene, electrophoretic mobility shift, and Western blotting assays showed that wogonin inhibits MMP-9 gene transcriptional activity by blocking the activation of NF-kappaB via MAPK signaling pathways
Ishiguro et al., Mol Immunol 2007 : S138A mutation prolongs Bcl10 induced NF-kappaB activation, suggesting that Bcl10 phosphorylation is involved in attenuation of NF-kappaB activation
Cappello et al., Arterioscler Thromb Vasc Biol 2007 (Atherosclerosis) : We showed that activation of NF-kappaB by lipopolysaccharide (LPS) , a prototypic inducer of innate immunity, was reversibly inhibited by ozLDL in monocytic THP-1 cells in a dose dependent manner, whereas tumor necrosis factor (TNF) signaling was not affected
Hernández-Vargas et al., Circ Res 2006 (Atherosclerosis) : Furthermore, the expression of monocyte chemoattractant protein-1 ( MCP-1 ), RANTES ( Regulated on Activated Normal T-cell Expressed and Secreted ), and intercellular adhesion molecule-1 ( ICAM-1 ) and the activation of nuclear factor-kappaB (NF-kappaB) were significantly reduced in aortic lesions from DKO mice ... Furthermore, the expression of monocyte chemoattractant protein-1 ( MCP-1 ), RANTES ( Regulated on Activated Normal T-cell Expressed and Secreted ), and intercellular adhesion molecule-1 ( ICAM-1 ) and the activation of nuclear factor-kappaB (NF-kappaB) were significantly reduced in aortic lesions from DKO mice
Choi et al., Immunology 2007 (Candidiasis...) : In studying the mechanisms underlying the susceptibility of the kidney to candidal infection, we previously reported that the reduced production of cytokines [ i.e. tumour necrosis factor-alpha (TNF-alpha) ] via platelet activating factor (PAF) induced activation of nuclear factor-kappaB (NF-kappaB) renders the organ susceptible to the fungal burden ... PAF induced early activation of NF-kappaB and TNF-alpha mRNA expression were both observed in the kidneys of mice infected with non-pathogenic strains of C. albicans, but not in mice infected with pathogenic strains ... Impairment of PAF mediated early NF-kappaB activation following infection with pathogenic C. albicans was associated with the prevention of activation of the enzyme cytosolic phospholipase A ( 2 ) ( cPLA(2) ) as well as the upstream pathway of cPLA(2), p38 mitogen activated protein kinase
Boyd et al., Cardiovasc Res 2006 (Myocarditis) : Using transfection of a nuclear factor kappa B (NF-kappaB)-Luciferase reporter plasmid, we found significantly increased NF-kappaB transcriptional activity in response to TLR2, TLR4 and TLR5 activation in cardiomyocytes ... Using transfection of a nuclear factor kappa B (NF-kappaB)-Luciferase reporter plasmid, we found significantly increased NF-kappaB transcriptional activity in response to TLR2 , TLR4 and TLR5 activation in cardiomyocytes ... Using transfection of a nuclear factor kappa B (NF-kappaB)-Luciferase reporter plasmid, we found significantly increased NF-kappaB transcriptional activity in response to TLR2, TLR4 and TLR5 activation in cardiomyocytes
Chua et al., Vaccine 2007 : Furthermore, the C16 lipopeptide was the most effective in activating dendritic cells, measured by up regulation of surface MHC Class II molecules, and also in activating NF-kappaB in a Toll-like receptor-2 (TLR2) dependent manner
Ge et al., Vascul Pharmacol 2007 : Since EMMPRIN and MMP-9 up-regulation is associated with activation of the NF-kappaB pathway, we investigated the effect of pioglitazone and resveratrol on TNF-alpha induced NF-kappaB activation
Shibata et al., Atherosclerosis 2007 : Mulberry leaf aqueous fractions inhibit TNF-alpha induced nuclear factor kappaB (NF-kappaB) activation and lectin-like oxidized LDL receptor-1 ( LOX-1 ) expression in vascular endothelial cells ... Furthermore, mulberry leaf aqueous fractions inhibited TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) and phosphorylation of inhibitory factor of NF-kappaB-alpha ( IkappaB-alpha ) in a time- and concentration dependent fashion
Loeuillet et al., J Immunol 2006 : In contrast, both a dominant negative construct of the MyD88 adaptor and an NF-kappaB inhibitor abrogated the protection against FasL mediated apoptosis, showing the implication of TLR2 mediated activation of NF-kappaB in apoptosis protection in infected macrophages
Rajaram et al., J Immunol 2006 (Gram-Negative Bacterial Infections) : Analysis of the mechanism of Akt influence on cytokine response demonstrated that Akt promotes NF-kappaB activation
Devauchelle et al., J Immunol 2006 (Arthritis, Rheumatoid...) : We propose that in RA joints, high levels of extracellular CLU and low expression of intracellular CLU may enhance NF-kappaB activation and survival of the synoviocytes
Nishitani et al., J Biol Chem 2006 : The TLR4 peptide inhibited the binding of a recombinant soluble form of the extracellular TLR4 domain ( sTLR4 ) to sMD-2 and significantly attenuated LPS induced NF-kappaB activation and IL-8 secretion in wild type TLR4 transfected cells
Lee et al., J Cell Physiol 2007 (Dermatitis, Contact...) : Silibinin also inhibited the LPS induced activation of MAPKs and the nuclear translocation of the NF-kappaB p65 subunit
Jiang et al., FASEB J 2006 (Colonic Neoplasms...) : Stable expression of IKBSR, a known repressor of NF-kappaB activation, and cellular caspase-8-like inhibitory protein ( cFLIP ) inhibited activation of NF-kappaB in cancer cell media treated muscle cells with an accompanying enhancement of myogenic protein expression and differentiation ... Stable expression of IKBSR, a known repressor of NF-kappaB activation, and cellular caspase-8-like inhibitory protein ( cFLIP ) inhibited activation of NF-kappaB in cancer cell media treated muscle cells with an accompanying enhancement of myogenic protein expression and differentiation
Kim et al., J Biol Chem 2006 (Down Syndrome...) : To elucidate the molecular basis of these anti-inflammatory effects, we analyzed the role of DSCR1 in the regulation of NF-kappaB transactivation using glioblastoma cells stably transfected with DSCR1.4 or its truncation mutants ( DSCR1.4- ( 1-133 ) and DSCR1.4- ( 134-197 ) ) ... Experiments using inhibitors of the signaling molecules for NF-kappaB activation showed that NF-kappaB is responsible for the induction of COX-2
Zheng et al., Zhonghua Yi Xue Za Zhi 2006 (Leukemia, Myelogenous, Chronic, BCR-ABL Positive) : STI571 could promote the activation/maturation of DC derived from BMMNCs of patients with CML in vitro, which might be partially responsible for the fact that the inhibitory effect of VEGF on DC NF-kappaB activation was relieved through STI571 inhibiting the overproduction of VEGF in CML
Kweon et al., Biochem Biophys Res Commun 2006 (Inflammation...) : Synergistic activation of NF-kappaB by nontypeable H. influenzae and S. pneumoniae is mediated by CK2, IKKbeta-IkappaBalpha, and p38 MAPK ... Synergistic activation of NF-kappaB by nontypeable H. influenzae and S. pneumoniae is mediated by CK2, IKKbeta-IkappaBalpha , and p38 MAPK ... Synergistic activation of NF-kappaB by nontypeable H. influenzae and S. pneumoniae is mediated by CK2, IKKbeta-IkappaBalpha , and p38 MAPK ... Moreover, casein kinase 2 ( CK2 ) is involved in synergistic induction of NF-kappaB via a mechanism dependent on phosphorylation of p65 at both Ser536 and Ser276 sites
Hohmann et al., Mol Cell Biol 1991 (Leukemia) : No correlation exists in HL60 cells between NF-kappa B activation by tumor necrosis factor ( TNF alpha ) and TNF beta and intracellular levels of cyclic AMP ... No correlation exists in HL60 cells between NF-kappa B activation by tumor necrosis factor ( TNF alpha ) and TNF beta and intracellular levels of cyclic AMP ... Forskolin or 1-isobutyl-3-methylxanthine drastically increased intracellular levels of cyclic AMP, but neither activated NF-kappa B nor influenced TNF induced NF-kappa B activation
Szabó et al., Diabetes 2006 (Body Weight...) : PARP blockade by INO-1001 and PJ-34 prevented hyperglycemia induced nuclear factor-kappaB (NFkappaB) activation of podocytes, and it was made evident by the inhibitor of kappaBalpha phosphorylation and NFkappaB p50 nuclear translocation
Ramanathan et al., Mol Biol Cell 2007 : HIF-1alpha mRNA levels were increased in LPS treated macrophages in an NF-kappaB dependent manner ; NECA strongly increased these levels in an A2AR dependent manner ... Deletion of putative NF-kappaB binding sites from the VEGF promoter did not affect LPS/NECA induced VEGF promoter activity, suggesting that NF-kappaB is not directly involved in VEGF transcription
Chiou et al., Pediatr Res 2006 : Activated-Akt can activate downstream signal protein [ p70S6 K, nuclear factor (NF)-kappaB , and extracellular signal regulated kinase ( ERK ) ], increasing the cyclin D1 level and suppressing the transcription of p27Kip1 to enable cell cycle entry
Zaidi et al., Immunology 2006 : In contrast, inhibitors of nuclear factor kappa B (NF-kappaB) completely blocked CCL2 production in MLMC and BMMC but not in LAD 2 mast cells
Kim et al., Immunology 2006 (Atherosclerosis...) : Stimulation of GITR caused nuclear factor (NF)-kappaB dependent activation of matrix metalloproteinase-9 (MMP-9) and pro-inflammatory cytokine expression in both the human and mouse monocytic/macrophage cell lines, THP-1 and RAW264.7, respectively ... Stimulation of GITR caused nuclear factor (NF)-kappaB dependent activation of matrix metalloproteinase-9 (MMP-9) and pro-inflammatory cytokine expression in both the human and mouse monocytic/macrophage cell lines, THP-1 and RAW264.7, respectively
Chen et al., Biochem Pharmacol 2007 (Lung Neoplasms) : Pretreatment of cells with tangeretin inhibited IL-1beta induced p38 MAPK, JNK, and AKT phosphorylation and the downstream activation of NF-kappaB ... Pretreatment of cells with tangeretin inhibited IL-1beta induced p38 MAPK, JNK, and AKT phosphorylation and the downstream activation of NF-kappaB
Frasca et al., Exp Gerontol 2007 : Therefore, NF-kappaB is not involved in the decreased response of old B cells to anti-CD40/IL-4 ... Therefore, NF-kappaB is not involved in the decreased response of old B cells to anti-CD40/IL-4
Jin et al., Pharmacol Res 2006 : Moreover, PIC prevented NF-kappaB p65 nuclear translocation
Zhou et al., J Surg Res 2007 (Body Weight...) : The results of the present study suggest that SAH in the rabbits could induce NF-kappaB and proinflammatory cytokines activation in the intestine, which is associated with morphological changes
Ivanov et al., Exp Cell Res 2006 (Melanoma) : Furthermore, activation of cJun and suppression of NF-kappaB by sodium arsenite resulted in upregulation of the endogenous TRAIL and downregulation of the cFLIP gene expression ( which encodes one of the main anti-apoptotic proteins in melanomas ) followed by cFLIP protein degradation and, finally, by acceleration of TRAIL induced apoptosis ... Furthermore, activation of cJun and suppression of NF-kappaB by sodium arsenite resulted in upregulation of the endogenous TRAIL and downregulation of the cFLIP gene expression ( which encodes one of the main anti-apoptotic proteins in melanomas ) followed by cFLIP protein degradation and, finally, by acceleration of TRAIL induced apoptosis
Ulanova et al., Biochem Biophys Res Commun 2006 : Inhibition of Syk down-regulated TNF induced p38 and p44/42 MAPK phosphorylation and nuclear translocation of p65 NF-kappaB ... Inhibition of Syk down-regulated TNF induced p38 and p44/42 MAPK phosphorylation and nuclear translocation of p65 NF-kappaB
Park et al., Carcinogenesis 2007 (Fibrosarcoma...) : MT1-MMP expression is regulated by NF-kappaB
Gilmore et al., Oncogene 2006 : NF-kappaB signaling is primarily regulated by inhibitor kappaB ( IkappaB ) proteins and the IkappaB kinase complex through two major pathways : the canonical and non-canonical NF-kappaB pathways
Dutta et al., Oncogene 2006 (Cell Transformation, Viral...) : Current insights into the regulation of programmed cell death by NF-kappaB ... However, improper regulation of PCD by NF-kappaB can have severe pathologic consequences, ranging from neurodegeneration to cancer, where its activity often precludes effective therapy
Zidi et al., Cell Mol Life Sci 2006 (Choriocarcinoma...) : We investigated the role of NF-kappaB in modulating HLA-G expression in HLA-G positive tumor cells, JEG-3 ( choriocarcinoma ), FON ( melanoma ), and M8-HLA-G1 ( HLAG1 transfected melanoma ) ... We investigated the role of NF-kappaB in modulating HLA-G expression in HLA-G positive tumor cells, JEG-3 ( choriocarcinoma ), FON ( melanoma ), and M8-HLA-G1 ( HLAG1 transfected melanoma )
Madrigal et al., CNS Neurol Disord Drug Targets 2006 (Atrophy) : NFkappaB activation results in the induction of iNOS and COX2 , two enzymes responsible for a great portion of the neurological damage produced in models of stress ... NFkappaB activation results in the induction of iNOS and COX2, two enzymes responsible for a great portion of the neurological damage produced in models of stress
Hirano et al., J Gastroenterol Hepatol 2006 : Luciferase assay showed that CDCA and TCDCA activated NF-kappaB-driven transcriptional activity
Berchtold et al., Mol Cell Biol 2007 (Calcium Signaling) : Overexpression of Ran in this cell model showed that DNA damage stimuli induced formation of a complex between Ran and NEMO, suggesting that RCC1 regulated NF-kappaB activation through the modulation of RanGTP
Olson et al., Infect Immun 2007 : p38 mitogen activated protein kinase controls NF-kappaB transcriptional activation and tumor necrosis factor alpha production through RelA phosphorylation mediated by mitogen- and stress activated protein kinase 1 in response to Borrelia burgdorferi antigens ... We now show that p38 MAP kinase activity regulates the transcriptional activation of NF-kappaB in response to spirochetal lysate stimulation of phagocytic cells
Xu et al., Arthritis Rheum 2006 (Arthritis, Rheumatoid) : RhoA mediated, tumor necrosis factor alpha induced activation of NF-kappaB in rheumatoid synoviocytes : inhibitory effect of simvastatin ... This study was conducted to examine the role of RhoA in mediating the activation of NF-kappaB in tumor necrosis factor alpha (TNFalpha) stimulated rheumatoid synoviocytes, and to evaluate the modulatory effects of statins on the TNFalpha induced activation of RhoA and NF-kappaB and the secretion of proinflammatory cytokines by rheumatoid synoviocytes ... This study was conducted to examine the role of RhoA in mediating the activation of NF-kappaB in tumor necrosis factor alpha (TNFalpha) stimulated rheumatoid synoviocytes, and to evaluate the modulatory effects of statins on the TNFalpha induced activation of RhoA and NF-kappaB and the secretion of proinflammatory cytokines by rheumatoid synoviocytes ... This study identifies RhoA as the key regulator of TNFalpha induced NF-kappaB activation, which ultimately results in the secretion of proinflammatory cytokines in rheumatoid synoviocytes
Li et al., World J Gastroenterol 2006 : Effects of IkappaBalpha and its mutants on NF-kappaB and p53 signaling pathways ... To study the effects of IkappaBalpha and its mutants ( IkappaBalphaM, IkappaBalpha243N, IkappaBalphaM244C ) on NF-kappaB , p53 and their downstream target genes ... Effects of IkappaBalpha and its mutants on the transprition level of NF-kappaB , NF-kappaB downstream target gene TNF-alpha, p53 and p53 downstream target gene Bax were observed by real time QT-PCR
Mentaverri et al., FASEB J 2006 : Similar to RANKL, Ca ( o ) 2+ ( 20 mM ) appeared to trigger rapid and significant nuclear translocation of NF-kappaB in a CaR- and PLC dependent manner
El-Rayes et al., Cancer Res 2006 (Pancreatic Neoplasms) : In the BxPC-3 cell line, significant down-regulation of EGFR, phosphorylated Akt , NF-kappaB activation , and survivin was observed in the cells treated with the combination compared with the erlotinib treated cells
Pantano et al., Am J Respir Cell Mol Biol 2007 : Previously we demonstrated that in cultured mouse lung epithelial cells exposed to bolus administration of H ( 2 ) O ( 2 ), TNF-alpha induced NF-kappaB activity was inhibited, whereas c-Jun-N-terminal kinase (JNK) activation was enhanced via a mechanism involving TNF receptor-1 ( TNF-RI ) ... Nox1 expression and activation inhibited TNF-alpha induced inhibitor of kappaB kinase (IKK), and NF-kappaB while promoting JNK activation and cell death
Nakata et al., Arterioscler Thromb Vasc Biol 2007 : Inhibition of Akt by dominant negative Akt suppressed atorvastatin induced nNOS expression as well as Akt and NF-kappaB activation ... Inhibition of NF-kappaB by dominant negative IkappaB also attenuated atorvastatin induced nNOS expression and NF-kappaB activation, but not Akt activation ... Inhibition of NF-kappaB by dominant negative IkappaB also attenuated atorvastatin induced nNOS expression and NF-kappaB activation, but not Akt activation
Chung et al., Toxicological sciences : an official journal of the Society of Toxicology 2007 : Upregulation of NF-kappaB and COX-2 expression by AcQ treatment was suppressed by the antioxidant N-acetylcysteine (NAC)
Riccioli et al., J Immunol 2006 : Moreover, we studied the role of NF-kappaB and of MAPKs in regulating TLR mediated MCP-1 secretion by using inhibitors specific for each transduction pathway and we demonstrated a pivotal role of the IkappaB/NF-kappaB and JNK systems
Schmidt et al., J Immunol 2006 : This phenotype correlates with Btk dependent induction of NF-kappaB and AP-1 DNA binding activity, and altered commensal bacteria populations
Liao et al., Neurochem Int 2007 : The inductive action of manganese was accompanied by generation of oxidative stress, activation of mitogen activated protein kinases ( MAPKs ), AKT , and protein kinase C-alpha (PKC-alpha), and increased NF-kappaB and AP-1 DNA binding activities ... The inductive action of manganese was accompanied by generation of oxidative stress, activation of mitogen activated protein kinases ( MAPKs ), AKT, and protein kinase C-alpha (PKC-alpha) , and increased NF-kappaB and AP-1 DNA binding activities
Lee et al., Nephrol Dial Transplant 2007 (Diabetes Mellitus, Experimental...) : COMP-Ang1 mediated inhibition of increased NF-kappaB-DNA binding in nuclear extracts from HUVECs grown in high glucose was significantly blocked by soluble Tie2 receptor-Fc ... COMP-Ang1 mediated inhibition of increased NF-kappaB-DNA binding in nuclear extracts from HUVECs grown in high glucose was significantly blocked by soluble Tie2 receptor-Fc
Sompol et al., J Mol Neurosci 2006 (Neuroblastoma) : In the present study, we demonstrate that TNF-alpha activates NF-kappaB activity in neuronal, SH-SY5Y, cells and preferentially enhances the binding of p50 and p65 to the promoter/enhancer regions of the MnSOD gene
Liu et al., Indian J Med Res 2006 (Myocardial Reperfusion Injury) : The suppression of proinflammatory cytokines expression may act by inhibiting NF-kB activation during I-R, but not by induction of IL-10
Wullaert et al., J Biol Chem 2007 : Although LPS induced activation of NF-kappaB is critical to the induction of an efficient immune response, excessive or prolonged signaling from TLR4 can be harmful to the host ... Similar to the other ABINs, ABIN-3 binds to A20 and inhibits NF-kappaB activation induced by tumor necrosis factor, interleukin-1, and 12-O-tetradecanoylphorbol-13-acetate ... Similar to the other ABINs, ABIN-3 binds to A20 and inhibits NF-kappaB activation induced by tumor necrosis factor, interleukin-1 , and 12-O-tetradecanoylphorbol-13-acetate ... Similar to the other ABINs, ABIN-3 binds to A20 and inhibits NF-kappaB activation induced by tumor necrosis factor , interleukin-1, and 12-O-tetradecanoylphorbol-13-acetate ... Moreover, in vivo adenoviral gene transfer of ABIN-3 in mice reduced LPS induced NF-kappaB activity in the liver, thereby partially protecting mice against LPS/D- ( + ) -galactosamine induced mortality ... Moreover, in vivo adenoviral gene transfer of ABIN-3 in mice reduced LPS induced NF-kappaB activity in the liver, thereby partially protecting mice against LPS/D- ( + ) -galactosamine induced mortality ... Taken together, these results implicate ABIN-3 as a novel negative feedback regulator of LPS induced NF-kappaB activation
Creely et al., Am J Physiol Endocrinol Metab 2007 (Diabetes Mellitus, Type 2...) : NF-kappaB inhibitor reduced IL-6 in AbdSc adipocytes ( Control : 2.7+/-0.5 vs. NF-kappaB inhibitor : 2.1+/-0.4 ng/ml ; P < 0.001 )
Yamaguchi et al., FEMS Immunol Med Microbiol 2007 (Actinobacillus Infections...) : A luciferase assay showed that adiponectin was able to inhibit the TLR4 mediated NF-kappaB activity in RAW264 cells
Klemm et al., Proc Natl Acad Sci U S A 2007 : Bcl10 and Malt1 control lysophosphatidic acid induced NF-kappaB activation and cytokine production ... Bcl10 and Malt1 control lysophosphatidic acid induced NF-kappaB activation and cytokine production ... Here, we identify the adapter proteins Bcl10 and Malt1 as essential mediators of LPA induced NF-kappaB activation ... By using murine embryonic fibroblasts from Bcl10- or Malt1-deficient mice as a genetic model, we report that Bcl10 and Malt1 are critically required for the degradation of IkappaB-alpha and the subsequent NF-kappaB induction in response to LPA stimulation ... By using murine embryonic fibroblasts from Bcl10- or Malt1-deficient mice as a genetic model, we report that Bcl10 and Malt1 are critically required for the degradation of IkappaB-alpha and the subsequent NF-kappaB induction in response to LPA stimulation ... By using murine embryonic fibroblasts from Bcl10- or Malt1-deficient mice as a genetic model, we report that Bcl10 and Malt1 are critically required for the degradation of IkappaB-alpha and the subsequent NF-kappaB induction in response to LPA stimulation ... By using murine embryonic fibroblasts from Bcl10- or Malt1-deficient mice as a genetic model, we report that Bcl10 and Malt1 are critically required for the degradation of IkappaB-alpha and the subsequent NF-kappaB induction in response to LPA stimulation ... Bcl10 and Malt1 cooperate with PKCs selectively for LPA induced NF-kappaB activation but are dispensable for the activation of the Jnk, p38, Erk MAP kinase, and Akt signaling pathways
Feng et al., Blood 2007 (Bone Resorption...) : SDX-308 decreased constitutive and RANKL stimulated NF-kappaB activation and osteoclast formation in an osteoclast cellular model, RAW 264.7 ... SDX-308 effectively suppressed TNF-alpha induced IKK-gamma and IkappaB-alpha phosphorylation and degradation and subsequent NF-kappaB activation in human multiple myeloma cells
Jhun et al., Biochem Biophys Res Commun 2006 : Moreover, inhibition of AMPK suppressed transcriptional activation of NF-AT and AP-1, but not NF-kappaB , in PMA/Io activated Jurkat cells
Guo et al., Peptides 2006 (MAP Kinase Signaling System) : Electrophoretic mobility shift assays and Western blotting revealed that Ang II , signaling via AT(1), produces a time dependent increase in NF-kappaB DNA binding and IkappaBalpha degradation ... These results also demonstrate that Ang II leads to MAPK phosphorylation and p38MAPK pathway induced NF-kappaB activation ... These results also demonstrate that Ang II leads to MAPK phosphorylation and p38MAPK pathway induced NF-kappaB activation ... These results also demonstrate that Ang II leads to MAPK phosphorylation and p38MAPK pathway induced NF-kappaB activation
Raymond et al., Osteoarthritis Cartilage 2007 (Arthritis) : MMP-1 gene expression was mirrored by increases in NF-kappaB gene expression, and inhibition of NF-kappaB nuclear translocation with dominant negative IkappaBalpha reduced IL-1beta dependent MMP-1 gene expression
Dimayuga et al., J Neuroimmunol 2007 (Inflammation) : However, LPS induced activation of NFkappaB , and release of TNFalpha and IL-6 were significantly attenuated in SOD1 overexpressing cells, as was the ability of microglia to induce toxicity in cultured neurons
Asagiri et al., Bone 2007 (Bone Resorption) : TRAF6 activates NF-kappaB , which is important for the initial induction of NFATc1
Ki et al., J Biol Chem 2007 : Our data indicate that, of the Galpha proteins coupled to S1P receptors, Galpha(12) specifically regulates NF-kappaB mediated COX-2 induction by S1P downstream of S1P ( 1 ), S1P(3), and S1P(5), in a process mediated by the JNK dependent ubiquitination and degradation of IkappaBalpha
Ishihara et al., Curr Pharm Des 2006 (Gastrointestinal Diseases...) : In the intestine, TLR dependent activation of NF-kappaB plays a vital role in maintaining epithelial homeostasis as well as regulating infections and inflammation, while dysregulation of TLR signaling is associated with the pathogenesis of inflammatory bowel diseases ( IBD )
Hosokawa et al., Clin Exp Immunol 2006 (Periodontitis) : Phosphatidylinositol 3-kinase (PI3K) and nuclear factor-kappaB (NF-kappaB) inhibitor inhibit both ICAM-1 and VCAM-1 expression induced by TWEAK ... Phosphatidylinositol 3-kinase (PI3K) and nuclear factor-kappaB (NF-kappaB) inhibitor inhibit both ICAM-1 and VCAM-1 expression induced by TWEAK
Beech et al., Arthritis Res Ther 2006 (Arthritis, Rheumatoid) : The transcription factor NFkappaB is differentially involved in this process as CXC chemokines but not CC chemokines are inhibited after overexpression of IkappaBalpha , the natural inhibitor of NFkappaB
McAllister-Lucas et al., Proc Natl Acad Sci U S A 2007 (Inflammation) : CARMA3/Bcl10/MALT1 dependent NF-kappaB activation mediates angiotensin II-responsive inflammatory signaling in nonimmune cells ... CARMA3/Bcl10/MALT1 dependent NF-kappaB activation mediates angiotensin II-responsive inflammatory signaling in nonimmune cells ... CARMA3/Bcl10/MALT1 dependent NF-kappaB activation mediates angiotensin II-responsive inflammatory signaling in nonimmune cells ... Much of this effect of Ang II results from activation of the proinflammatory NF-kappaB transcription factor in response to stimulation of the type 1 Ang II receptor, a G protein coupled receptor ... Here, we characterize a previously undescribed signaling pathway mediating Ang II-dependent activation of NF-kappaB , which is composed of three principal proteins, CARMA3, Bcl10, and MALT1
Liu et al., J Virol 2007 : Short interfering RNA ( siRNA ) -mediated RIG-I `` knockdown '' significantly inhibited early nuclear factor-kappaB (NF-kappaB) and interferon response factor 3 (IRF3) activation 9 h postinfection ( p.i. ) ... In contrast, siRNA mediated TLR3 knockdown did not affect RSV induced NF-kappaB binding but did inhibit IFN-beta, IP-10, CCL-5 , and ISG15 expression at late times of infection ... In contrast, siRNA mediated TLR3 knockdown did not affect RSV induced NF-kappaB binding but did inhibit IFN-beta, IP-10 , CCL-5, and ISG15 expression at late times of infection ... In contrast, siRNA mediated TLR3 knockdown did not affect RSV induced NF-kappaB binding but did inhibit IFN-beta, IP-10, CCL-5, and ISG15 expression at late times of infection ... In contrast, siRNA mediated TLR3 knockdown did not affect RSV induced NF-kappaB binding but did inhibit IFN-beta , IP-10, CCL-5, and ISG15 expression at late times of infection
Kim et al., Cancer Res 2006 (Breast Neoplasms) : We recently showed that TGase 2 activated NF-kappaB through polymerization and depletion of free IkappaBalpha during inflammation ... Therefore, increased expression of TGase 2 and subsequent activation of NF-kappaB may contribute to drug resistance in breast cancer cells independently of EGF signaling
Kim et al., J Pharmacol Exp Ther 2007 : THI 53 also inhibited LPS mediated interferon (IFN)-beta production and nuclear factor-kappaB (NF-kappaB) activation
Keslacy et al., Mol Pharmacol 2007 (Inflammation) : Moreover, IFNgamma failed to affect TNFalpha induced IkappaKbeta phosphorylation or IkappaB degradation as well as nuclear NF-kappaB/DNA interaction
Horovitz-Fried et al., Biochem Biophys Res Commun 2007 : We earlier showed that insulin did not affect nuclear NFkappaB levels ... Thus, PKCalpha regulates insulin induced PKCdelta expression levels and this regulation involves activation of SP-1 and NFkappaB ... Thus, PKCalpha regulates insulin induced PKCdelta expression levels and this regulation involves activation of SP-1 and NFkappaB
Sethi et al., Blood 2007 (Neoplasm Invasiveness...) : Celastrol, a novel triterpene, potentiates TNF induced apoptosis and suppresses invasion of tumor cells by inhibiting NF-kappaB regulated gene products and TAK1 mediated NF-kappaB activation ... Recent studies indicate that TNF induced IKK activation requires activation of TAK1, and we indeed found that celastrol inhibited the TAK1 induced NF-kappaB activation
Zhang et al., J Appl Physiol 2007 (Hepatopulmonary Syndrome) : Hepatopulmonary syndrome (HPS) following rat common bile duct ligation results from pulmonary molecular changes that may be influenced by circulating TNF-alpha and increased vascular shear stress, through activation of NF-kappaB or Akt ... These changes were associated with a reduction in circulating TNF levels and NF-kappaB activation and complete inhibition of Akt activation
Ahn et al., J Biol Chem 2007 : Although gamma-tocotrienol completely abolished tumor necrosis factor alpha (TNF) induced NF-kappaB activation, a similar dose of gamma-tocopherol had no effect ... Besides TNF, gamma-tocotrienol also abolished NF-kappaB activation induced by phorbol myristate acetate, okadaic acid, lipopolysaccharide, cigarette smoke, interleukin-1beta , and epidermal growth factor ... Besides TNF, gamma-tocotrienol also abolished NF-kappaB activation induced by phorbol myristate acetate, okadaic acid, lipopolysaccharide, cigarette smoke, interleukin-1beta, and epidermal growth factor ... Besides TNF, gamma-tocotrienol also abolished NF-kappaB activation induced by phorbol myristate acetate, okadaic acid, lipopolysaccharide, cigarette smoke, interleukin-1beta , and epidermal growth factor
Cummins et al., Proc Natl Acad Sci U S A 2006 : Conversely, overexpression of PHD-1 decreases cytokine stimulated NFkappaB reporter activity, further suggesting a repressive role for PHD-1 in controlling the activity of NFkappaB
Ness et al., J Immunol 2006 (Bacterial Infections...) : MyD88 dependent NF-kappaB signaling was significantly down-regulated in CCR4 ( -/- ) macrophages, whereas p38 MAPK and JNK activation were conversely increased
Garaude et al., J Immunol 2006 (Leukemia) : shERK5 decreased nuclear accumulation of the NF-kappaB p65 subunit, and conversely, ectopic activation of ERK5 led to constitutive nuclear localization of p65 and increased its ability to trans activate specific reporter genes
Qin et al., J Immunol 2006 : We previously demonstrated that LPS induction of CD40 in macrophages/microglia involves both NF-kappaB activation and LPS induced production of IFN-beta, which subsequently activates STAT-1alpha ... We previously demonstrated that LPS induction of CD40 in macrophages/microglia involves both NF-kappaB activation and LPS induced production of IFN-beta, which subsequently activates STAT-1alpha ... IL-10 inhibits LPS induced IFN-beta gene expression and subsequent STAT-1alpha activation, but does not affect NF-kappaB activation
Pathak et al., J Immunol 2006 : TLR4 dependent NF-kappaB activation and mitogen- and stress activated protein kinase 1-triggered phosphorylation events are central to Helicobacter pylori peptidyl prolyl cis-, trans-isomerase ( HP0175 ) -mediated induction of IL-6 release from macrophages ... HP0175 induced IL-6 gene expression was critically dependent on TLR4 dependent NF-kappaB and MAPK activation ... HP0175 induced IL-6 gene expression was critically dependent on TLR4 dependent NF-kappaB and MAPK activation ... The central role of MSK1 was borne out by the fact that silencing of MSK1 expression abrogated HP0175 mediated NF-kappaB dependent IL-6 gene transcription
O'Hara et al., J Immunol 2006 (Helicobacter Infections) : Small interfering RNA mediated silencing of APE-1/Ref-1 inhibited basal and H. pylori induced AP-1 and NF-kappaB DNA binding activity without affecting the nuclear translocation of these transcription factors and also reduced H. pylori induced IL-8 mRNA and protein
Fernández-Figueras et al., Mod Pathol 2007 (Carcinoma, Merkel Cell...) : We found statistically significant correlations between metastatic tumor spread and overexpression of matrix metalloproteinase (MMP) 7 , MMP10/2, tissue inhibitor of metalloproteinase 3, vascular endothelial growth factor ( VEGF ), P38, stromal NF-kappaB , and synaptophysin
Elmali et al., Inflammation 2007 (Arthritis, Experimental...) : Resveratrol, a polyphenolic, natural phytoalexin found with particularly high levels in grape skin and red wine is potent and specific inhibitor of TNF-alpha and IL-1beta induced NF-kappaB activation ... Resveratrol, a polyphenolic, natural phytoalexin found with particularly high levels in grape skin and red wine is potent and specific inhibitor of TNF-alpha and IL-1beta induced NF-kappaB activation
Hansberger et al., J Virol 2007 : Using IKK subunit-specific small interfering RNAs and cells deficient in individual IKK subunits, we demonstrate that IKKalpha but not IKKbeta is required for reovirus induced NF-kappaB activation and apoptosis ... Using IKK subunit-specific small interfering RNAs and cells deficient in individual IKK subunits, we demonstrate that IKKalpha but not IKKbeta is required for reovirus induced NF-kappaB activation and apoptosis ... Collectively, these findings indicate a novel pathway of NF-kappaB/Rel activation involving IKKalpha and IKKgamma/Nemo, which together mediate the expression of downstream proapoptotic genes in reovirus infected cells ... Collectively, these findings indicate a novel pathway of NF-kappaB/Rel activation involving IKKalpha and IKKgamma/Nemo , which together mediate the expression of downstream proapoptotic genes in reovirus infected cells
Yoon et al., Mol Cancer Ther 2006 (Breast Neoplasms...) : H-Ras did not increase MMP-9 in the presence of a PI3K inhibitor, LY294002, and a NF-kappaB inhibitor, SN50
Tsai et al., Anesthesiology 2006 : The effects of HO-1 induction on nuclear factor E2-related factor 2 ( Nrf2 ) and nuclear factor kappaB (NF-kappaB) were also investigated
Gao et al., Inflamm Res 2006 : LPS also significantly increased mRNA of iNOS, protein level of NF-kappaB and decreased mRNA of eNOS ( P < 0.05 )
Yi et al., Inflamm Res 2006 (Endotoxemia...) : Lung injury score, lung wet-to-dry weight ( W/D ) ratio, percentage of neutrophils ( PMNs ) in BALF, lung permeability index (LPI) , intercellular adhesion molecule-1 ( ICAM-1 ) expression and activation of nuclear factor-kappa B (NF-kappaB) in the lungs were determined ... Lung injury score, lung wet-to-dry weight ( W/D ) ratio, percentage of neutrophils ( PMNs ) in BALF, lung permeability index (LPI), intercellular adhesion molecule-1 ( ICAM-1 ) expression and activation of nuclear factor-kappa B (NF-kappaB) in the lungs were determined ... Lung injury score, lung wet-to-dry weight ( W/D ) ratio, percentage of neutrophils ( PMNs ) in BALF, lung permeability index (LPI), intercellular adhesion molecule-1 ( ICAM-1 ) expression and activation of nuclear factor-kappa B (NF-kappaB) in the lungs were determined
Rasoulpour et al., Biol Reprod 2007 (Disease Susceptibility...) : Inhibiting activity of one of the catalytic subunits of the IKK complex, IKKbeta ( also known as IKBKB and IKK2 ) severely inhibits NF-kappaB nuclear translocation in response to most stimuli, including ionizing radiation
Heynekamp et al., J Med Chem 2006 : In the present study, phenolic analogues of resveratrol and a series of substituted trans-stilbenes without hydroxy groups were compared with resveratrol for their abilities to inhibit the human tumor necrosis factor alpha induced ( TNF-alpha ) activation of NF-kappaB , using the Panomics NF-kappaB stable reporter cell line 293/NF-kappaB-luc
Sedimbi et al., Ann N Y Acad Sci 2006 (Autoimmune Diseases...) : The novel polymorphism M55V, causing an amino acid change in the evolutionarily conserved met55 residue has been shown to activate the nuclear factor kappaB (NF-kappaB) , hence the suspected role of SUMO4 in the pathogenicity of T1DM
Kim et al., Immunol Cell Biol 2007 : The synergistic action of 15d-PGJ ( 2 ) on LPS induced MIP-2 ( CXCL2 ) mRNA expression was dependent on the activation of nuclear factor-kappaB (NF-kappaB) , and 15d-PGJ ( 2 ) increased the phosphorylation of p38 and stress activated protein kinase/c-Jun N-terminal kinase ( SAPK/JNK ) in cells stimulated with LPS
Jin et al., J Cell Biochem 2007 (Muscular Atrophy) : LPS activated p38 and NF-kappaB , while inhibiting AKT ; whereas, curcumin administration inhibited LPS stimulated p38 activation, without altering the effect of LPS on NF-kappaB and AKT
Traore et al., J Cell Biochem 2007 (Leukemia, Myeloid) : In addition, TPA induced ICAM-1 expression was blocked by inhibition of nuclear factor-kappaB (NF-kappaB) activation following pretreatment with BAY11-7085 ( a specific inhibitor of NF-kappaB activation )
Armstrong et al., Neoplasia (New York, N.Y.) 2006 (Brain Neoplasms...) : This increase occurs before the degradation of inhibitor of NF-kappaB (I-kappaB) alpha and the NF-kappaB dependent activation of gene transcription ... Moreover, p53 is necessary for NF-kappaB activation because cells with inactive p53 were resistant to NF-kappaB mediated cell death
Furusu et al., J Cell Physiol 2007 : In this report, we examined roles of nuclear factor-kappaB (NF-kappaB) and mitogen activated protein ( MAP ) kinases in tumor necrosis factor-alpha (TNF-alpha) induced expression of IAP genes
Duggan et al., J Cell Physiol 2007 : Role of atypical protein kinase C isozymes and NF-kappaB in IL-1beta induced expression of cyclooxygenase-2 in human myometrial smooth muscle cells
Chen et al., Am J Physiol Cell Physiol 2007 : ROS and NF-kappaB but not LXR mediate IL-1beta signaling for the downregulation of ATP binding cassette transporter A1 ... In contrast, NF-kappaB inhibition by pyrrolidine dithiocarbamate and MG132 prevented the suppression of ABCA1 by IL-1beta ... Thus our data provide strong evidence that ROS and NF-kappaB , but not LXR, mediate the IL-1beta induced downregulation of ABCA1 via a novel transcriptional mechanism, which might play an important role of proinflammation in the alteration of lipid metabolism
Tsuchiya et al., Laryngoscope 2007 : However, the PGPS induced IL-8 promoter activation in rodent middle ear epithelial cells required NF-kappaB and NF-IL6 but not AP-1
Parhar et al., Mol Cell Biochem 2007 : Investigation of interleukin 1beta mediated regulation of NF-kappaB activation in colonic cells reveals divergence between PKB and PDK transduced events ... We have investigated the role of PKB and PDK1 in IL-1beta induced NF-kappaB activation ... We have investigated the role of PKB and PDK1 in IL-1beta induced NF-kappaB activation ... We have investigated the role of PKB and PDK1 in IL-1beta induced NF-kappaB activation ... N-tosyl phenylalanyl chloromethyl ketone ( TPCK ), wortmannin and Ly294002 inhibited IL-1beta induced NF-kappaB activation in both systems indicating involvement of the PI3K axis in this response ... Using a p65-S536A reporter construct, we found inhibition of only PDK1 over-expression induced, but not PKB over-expression induced NF-kappaB activation ... Our data reveals for the first time that PDK1 and PKB may differentially activate NF-kappaB , and that TPCK may subserve a useful anti-inflammatory function by inhibiting IKKbeta ... Our data reveals for the first time that PDK1 and PKB may differentially activate NF-kappaB , and that TPCK may subserve a useful anti-inflammatory function by inhibiting IKKbeta
Li et al., Cardiovasc Res 2007 (Cardiomegaly) : Ad.GSK-3beta-KM abrogated kallikrein 's actions on GSK-3beta and CREB phosphorylation and NF-kappaB activation, whereas icatibant blocked all kallikrein 's effects
Kilpatrick et al., J Leukoc Biol 2006 (MAP Kinase Signaling System) : delta-PKC associates with TNFR-1 in response to TNF and is required for NFkappaB activation and inhibition of caspase 3 ... delta-PKC associates with TNFR-1 in response to TNF and is required for NFkappaB activation and inhibition of caspase 3
Peng et al., Exp Cell Res 2007 (Inflammation...) : Furthermore, the production of IL-8 in PMNs is NF-kappaB dependent
Sheng et al., FEBS Lett 2006 : Analysis of the hTERT promoter occupancy in vivo using chromatin immunoprecipitation assays, however, did not detect an increased binding of NFkappaB to the hTERT promoter in the activated T cells, although an increased binding of cMyc and Sp1 was detected
Higai et al., Biol Pharm Bull 2006 (Adenocarcinoma...) : TNFalpha stimulation induced the biphasic increases in expression of NFkappaB-p65 , ST3Gal I, FUT IV, ST3Gal IV and ST6GalNAc III mRNAs and the transient increase in expression of ST6Gal I mRNA and the decrease in ST3GalNAc IV mRNA
Uto et al., Oncol Rep 2007 : LPS induced activation of all of these factors and 6-MITC suppressed LPS induced activation of AP-1, C/EBPdelta, CREB, but not NF-kappaB
Song et al., World J Gastroenterol 2006 (Bacterial Infections...) : In addition, CLP increased hepatic NF-kappaB expression compared with sham CLP
Wolfert et al., Infect Immun 2007 : The compounds that induced synergistic effects were also able to activate NF-kappaB in a NOD1- or NOD2 dependent manner, implicating these proteins in synergistic TNF-alpha secretion ... It was found that a diaminopimelic acid (DAP) containing muramyl tetrapeptide could activate NF-kappaB in a NOD1 dependent manner, demonstrating that an exposed DAP is not essential for NOD1 sensing
Depboylu et al., J Neuropathol Exp Neurol 2006 (Disease Models, Animal...) : Treatment of AIDS monkeys with the central nervous system-permeant 6-chloro-2',3'-dideoxyguanosine fully reversed SIV burden, productive inflammation, nuclear NF-kappaB translocation as well as focal induction of CX3CL1 in astrocytes and macrophages and downregulation in neurons
Yu et al., Gut 2007 (Hepatitis, Animal) : Hepatic COX-2 expression in the TG mice resulted in activation of NF-kappaB and inflammatory cytokine cascade, with a marked expression of the proinflammatory cytokines tumour necrosis factor (TNF)-alpha ( 9.4-fold ), interleukin (IL)-6 ( 4.4-fold ), IL-1beta ( 3.6-fold ), and of the anti-inflammatory cytokine IL-10 ( 4.4-fold ) and chemokine macrophage inflammatory protein-2 ( 3.2-fold )
Bertolani et al., Am J Pathol 2006 (Acute Disease...) : The intracellular calcium chelator BAPTA-AM blocked resistin induced NF-kappaB activation and monocyte chemoattractant protein-1 expression
Bu et al., Am J Pathol 2006 (Carotid Artery Injuries) : The present study reveals a previously unrecognized vascular response to an-gioplastic injury, characterized by NF-kappaB dependent expression of NGAL in vascular SMCs. Further-more, SMC produced NGAL interacts with MMP-9, a mechanism by which NGAL may modulate MMP-9 proteolytic activity in the vascular repair process
Cardoso et al., Mol Cancer Ther 2006 (Breast Neoplasms) : These two pharmacologic agents prevent nuclear factor-kappaB (NF-kappaB) activation and induce nuclear accumulation of the cyclin dependent kinase inhibitor p27 ( kip1 ), suggesting that combining bortezomib with trastuzumab could increase trastuzumab efficacy
Ozeki et al., J Neurosci Res 2007 : Truncation of the NF-kappaB binding site in the cyclin D1 promoter fully abrogated the transcription of cyclin D1, suggesting that the cyclin D1 transcription is dependent on NF-kappaB
Fukuda et al., Nucleic Acids Symp Ser 2006 : TLR3 mediates the activation of IRF-3 and NF-kappaB and thereby the secretion of type I interferons and inflammatory cytokines
Kaileh et al., J Biol Chem 2007 (Inflammation) : In this study we show that the leave extract of WS, as well as its major constituent withaferin A ( WA ), potently inhibits NFkappaB activation by preventing the tumor necrosis factor induced activation of IkappaB kinase beta via a thioalkylation-sensitive redox mechanism, whereas other WS-derived steroidal lactones, such as withanolide A and 12-deoxywithastramonolide, are far less effective
Nonn et al., Carcinogenesis 2007 : We utilized the cytokines tumor necrosis factor-alpha (TNFalpha) and interleukin (IL)-1beta to increase p38 dependent nuclear factor kappa-B (NFkappaB) activation and expression of pro-inflammatory genes cyclooxygenase-2 (COX-2), IL-6 and IL-8 in normal prostatic epithelial cells
Hassan et al., BMC cancer 2006 (Neuroblastoma) : Activation of nuclear factor (NF)-kappaB by LPS was detected by degradation of IkappaB-alpha and NF-kappaB luciferase assay ... However, LPS did not significantly induce NF-kappaB activation in NB-1 cells although it slightly degraded IkappaB-alpha ... However, LPS did not significantly induce NF-kappaB activation in NB-1 cells although it slightly degraded IkappaB-alpha
Kobori et al., Br J Pharmacol 2007 (Adenocarcinoma...) : Ergosterol peroxide and ergosterol suppressed LPS induced DNA binding activity of NF-kappaB and C/EBPbeta, and inhibited the phosphorylation of p38 , JNK and ERK MAPKs ... Ergosterol peroxide and ergosterol suppressed LPS induced DNA binding activity of NF-kappaB and C/EBPbeta, and inhibited the phosphorylation of p38, JNK and ERK MAPKs
Kurbanov et al., Oncogene 2007 (Melanoma) : Here we show for the first time that TRAIL induced activation of NF-kappaB occurs in apoptosis-sensitive melanoma cell lines through TRAIL receptor 1/death receptor 4 ( TRAIL-R1/DR4 ), whereas TRAIL failed to activate nuclear factor kappa B (NF-kappaB) in melanoma cells positive only for TRAIL receptor 2/death receptor 5 ( TRAIL-R2/DR5 ) ... However, activation of NF-kappaB by TRAIL was not associated with enhanced expression of antiapoptotic factors : cellular FLICE-inhibitory protein ( c-FLIP ), Bcl-x ( L ), X-linked inhibitor of apoptosis protein ( XIAP ), Survivin, Livin
Asakura et al., Am J Hematol 2007 : Here we show that ectopic overexpression of catalytically inactive dominant negative PKR expression system suppressed NF- kappa B activation mediated by TLR3, TLR9 , TNF receptor 1 and 2 (TNF-R 1/2), but not by TLR4 ... Here we show that ectopic overexpression of catalytically inactive dominant negative PKR expression system suppressed NF- kappa B activation mediated by TLR3, TLR9, TNF receptor 1 and 2 (TNF-R 1/2) , but not by TLR4
Cho et al., Immunol Lett 2007 (Arthritis, Rheumatoid) : Moreover PI3K/Akt inhibitor, NF-kappaB inhibitor, and FK506 significantly inhibited IL-15 induced IL-17 production in CD4+ T cells
Flati et al., Int J Immunopathol Pharmacol 2006 : Furthermore, we found that bFGF inhibits the TNF mediated activation of NF-kappaB by blocking phosphorylation and degradation of IkappaBalpha ... Furthermore, we found that bFGF inhibits the TNF mediated activation of NF-kappaB by blocking phosphorylation and degradation of IkappaBalpha ... Furthermore, we found that bFGF inhibits the TNF mediated activation of NF-kappaB by blocking phosphorylation and degradation of IkappaBalpha ... We also found that bFGF induces hyperphosphorylation of p38 MAPK on endothelial cells, whereas inhibition of such kinase abrogates the effect of bFGF on the TNF mediated activation of NF-kappaB
Yu et al., Croat Med J 2006 : To investigate whether ketamine suppresses lipopolysaccharide (LPS) induced increase in Toll-like receptor 4 (TLR4) expression and nuclear factor-kappa B (NF-kappaB) activity in the intestines of rats ... Ketamine at the dosage of 0.5, 2.5, and 10 mg/kg suppressed the LPS induced increase in TLR4 expression and NF-kappaB activity ... The study demonstrated that ketamine inhibits NF-kappaB activation in the intestines of LPS treated rats, possibly by suppressing TLR4 expression
Tintut et al., J Investig Med 2006 (Atherosclerosis...) : Binding of RANKL to its cognate receptor RANK induces NF-kappaB signaling, which stimulates osteoclastic differentiation in preosteoclasts and induces bone morphogenetic protein ( BMP-2 ) expression in chondrocytes ... Binding of RANKL to its cognate receptor RANK induces NF-kappaB signaling, which stimulates osteoclastic differentiation in preosteoclasts and induces bone morphogenetic protein ( BMP-2 ) expression in chondrocytes
Di Marco et al., Nucleic Acids Res 2007 : We have previously shown that GILZ binds to and inhibits NF-kappaB activity
Deng et al., Wei Sheng Wu Xue Bao 2006 : The effects of pyrrolidine dithiocarbamate ( PDTC ), an inhibitor of NF-kappaB and of cycloheximide ( CHX ), a protein synthase inhibitor, on the expression of iNOS and on the activation of NF-kappaB were also investigated in mouse macrophages treated with LAMPs
Sun et al., Biochem Biophys Res Commun 2007 (Mechanotransduction, Cellular) : Involvement of integrins, MAPK, and NF-kappaB in regulation of the shear stress induced MMP-9 expression in endothelial cells ... Inhibition of NF-kappaB resulted in remarkable downregulation of stress induced MMP-9 expression ... Our findings demonstrated that the shear stress induced MMP-9 expression involved integrins-p38 MAPK or ERK1/2-NF-kappaB signaling pathways
Ramana et al., Cytokine 2006 : The LPS induced DNA binding activity of NF-kappaB and AP1 were significantly inhibited by AR inhibitors, and this effect was mediated through the inhibition of phosphorylation of IkappaB-alpha, IKK alpha/beta and PKC
Lee et al., Int Immunopharmacol 2007 (Colitis) : Simvastatin ( 50 micro M ) blocked TNF-alpha induced NF-kappaB transcriptional activity, IkappaB phosphorylation/degradation and DNA binding activity of NF-kappaB
Orlandi et al., J Biol Chem 2007 : Inhibiting NF-kappaB activation through SN50 increased apoptotic rate and Bax expression in intimal but not in medial SMCs, and successive PLC treatment failed to induce a further increase in apoptotic rate
Moreau et al., Mol Pharmacol 2007 : Lys-des-Arg9-bradykinin had little effect on NF-kappaB activation, the Bmax, or receptor mRNA abundance or stability
Wang et al., Proc Natl Acad Sci U S A 2007 : Bcl10 plays a critical role in NF-kappaB activation induced by G protein coupled receptors
Arumugam et al., J Natl Cancer Inst 2006 (Neoplasm Invasiveness...) : In vitro, cromolyn ( 100 microM ) inhibited S100P stimulated Panc-1 cell proliferation ( S100P, mean = 0.93 U, versus S100P + cromolyn, mean = 0.56 U, difference = 0.37 U ; 95 % confidence interval [ CI ] = 0.24 to 0.49 U ; P = .001, n = 3 ), invasion ( S100P, mean = 58.0 %, versus S100P + cromolyn, mean = 9.4 %, difference = 48.6 % ; 95 % CI = 38.8 % to 58.8 % ; P < .001, n = 3 ), and NFkappaB activity ( S100P, mean = 14,460, versus S100P + cromolyn, mean = 7360 photons/s, difference = 7100 photons/s ; 95 % CI = 3689 to 10 510 photons/s ; P = .005, n = 3 )
Cheng et al., Biochem Pharmacol 2007 (Endotoxemia...) : The inhibitory effects of baicalein on iNOS production may be mediated by inhibition of the activation of NF-kappaB
Nakamichi et al., J Immunol 2007 (Bone Resorption) : Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts ... OPG or a soluble form of receptor activator of NF-kappaB ( the receptor of RANKL ) suppressed the release of RANKL from those cells
Pietilä et al., J Immunol 2007 : Multiple NF-kappaB and IFN regulatory factor family transcription factors regulate CCL19 gene expression in human monocyte derived dendritic cells
Garcia et al., J Virol 2007 : The amino-terminal and central proline-rich motifs of STP-A were responsible for TRAF6 and TRAF2 interactions, respectively, and STP-A and TRAF6 interaction contributed to the majority of NF-kappaB activation, whereas STP-A and TRAF2 interaction played a minor role in NF-kappaB activation ... The amino-terminal and central proline-rich motifs of STP-A were responsible for TRAF6 and TRAF2 interactions, respectively, and STP-A and TRAF6 interaction contributed to the majority of NF-kappaB activation, whereas STP-A and TRAF2 interaction played a minor role in NF-kappaB activation
Boutet et al., J Dairy Sci 2007 (Mastitis, Bovine) : Prolactin activated NF-kappaB ; maximal NF-kappaB activation was weaker with PRL than with TNF-alpha at 30 or 180 min poststimulation
Kim et al., Antioxid Redox Signal 2007 : Effects of PGC-1alpha on TNF-alpha induced MCP-1 and VCAM-1 expression and NF-kappaB activation in human aortic smooth muscle and endothelial cells ... Effects of PGC-1alpha on TNF-alpha induced MCP-1 and VCAM-1 expression and NF-kappaB activation in human aortic smooth muscle and endothelial cells ... Consequently, NF-kappaB activity and MCP-1 and VCAM-1 induced by TNF-alpha are suppressed
Carlson et al., Am J Physiol Gastrointest Liver Physiol 2007 (MAP Kinase Signaling System) : fMLP pretreatment also inhibited activation of proinflammatory transcription factor NF-kappaB by TNF-alpha in Caco2bbe cells, reducing induction of NF-kappaB target genes by TNF-alpha both in human intestinal biopsies and Caco2bbe cells
Han et al., Phytother Res 2007 : In addition, KS-Fs and Kur were able to inhibit TNFalpha induced NF-kappaB activation in 293 cells mediated by the decreased IkappaBalpha phosphorylation
Fukuyama et al., Mol Carcinog 2007 (Colorectal Neoplasms) : However, it is not fully understood to what extent NFkappaB contributes to induction of the metastasis associated matrix metalloprotease-9 (MMP-9) gene and sensitivity to the commonly used chemotherapeutic 5-fluorouracil ( 5-Fu ) in CRC ... NFkappaB plays a predominant role in MMP-9 gene induction in RKO cells, as evidenced by the failure of tumor necrosis factor alpha (TNFalpha) to induce MMP-9 in either of the NFkappaB signaling mutants ... RKO cells exhibit a robust, oscillatory NFkappaB activity in response to TNFalpha not seen in either of the NFkappaB mutant cell lines, which instead demonstrate diminished, nonoscillatory NFkappaB activation ... Our results suggest that TNFalpha dependent induction of MMP-9 gene expression is tightly regulated by oscillatory/cumulative activation of NFkappaB and that 5-Fu stimulates NFkappaB and RKO CRC cell survival through induction of IKK activity
Kim et al., Immunopharmacol Immunotoxicol 2006 (Cerebral Infarction) : In addition, we show that SHJKS suppressed nuclear factor (NF)-kappaB activation induced by LPS plus PHA, leading to suppression of IkappaB-alpha phosphorylation and degradation
Ohga et al., American journal of physiology. Renal physiology 2007 (Diabetes Mellitus, Experimental...) : High-glucose conditions increased the expression of ICAM-1 and the activation of NF-kappaB in cultured glomerular endothelial cells
Arima et al., J Virol 1991 : Tax induction of the nuclear expression of c-Rel and NF-kappa B p50 is regulated, at least in part, at a pretranslational level involving increases in c-rel and NF-kappa B p105 mRNA expression
Oh et al., J Biomed Sci 2007 : HeLa cells treated with phytoglycoprotein ( 150 kDa ) were killed by activation of caspase 3 via inhibitory activities of NF-kappaB and AP-1
Jang et al., J Cell Physiol 2007 (Osteosarcoma) : Results from electrophoretic mobility shift assay ( EMSA ) showed that LZIP enhanced the Lkn-1 induced DNA binding activity of NF-kappaB
Indoh et al., Virology 2007 (Measles) : Infected monocytic cells exhibited decreased LPS induced DNA binding of NF-kappaB and phosphorylation of JNK, namely activation of transcription factors NF-kappaB and AP-1
Sen et al., Biochim Biophys Acta 2007 : NFkappaB p65 and IkappaB kinase (IKK) became phosphorylated and NFkappaB transcriptional activity became augmented during repetitive stress ... NFkappaB p65 and IkappaB kinase (IKK) became phosphorylated and NFkappaB transcriptional activity became augmented during repetitive stress
Yamabe et al., Nephrology (Carlton) 2006 : We also examined the effect of ROX on tyrosine kinase, MAP kinase and NF-kappaB stimulated by thrombin ... ROX ( 10.0 microg/mL ) suppressed the thrombin induced NF-kappaB activation, although ROX did not inhibit the activation of tyrosine kinase and MAP kinase by thrombin
Pandey et al., RNA Biol 2004 (Carcinoma...) : We checked the stress-inducible transcription factor, nuclear factor kappa B (NF-kappaB), which was persistently activated by cycloheximide but not by other agents after 24 hours indicating no role of NFkappaB in the RNase L-induction ... However, as expected, TNF induced NF-kappaB activity was stimulated within 10-30 minutes through degradation of IkappaB-alpha
Nascimento-Silva et al., Thromb Haemost 2007 (Cardiovascular Diseases) : Western blot and immunofluorescence microscopy analyses showed that ATL-1 ( 100 nM ) impaired the redox-sensitive activation of the transcriptional factor NF- kappaB , a critical step in several events associated to vascular pathologies
Hsu et al., J Gastroenterol Hepatol 2007 (Fibrosis...) : Tetrandrine ( 0.5-5.0 micromol/L ) concentration-dependently inhibited NFkappaB transcriptional activity induced by TNF-alpha , including IkappaBalpha phosphorylation and mRNA expressions of ICAM-1 in HSC-T6 cells
D'Alimonte et al., J Immunol 2007 : Moreover, guanosine abrogated IFN-gamma induced phosphorylation on Ser ( 727 ) and translocation of STAT-1alpha to the nucleus as well as TNF-alpha-/Abeta induced IkappaBalpha and NF-kappaB p65/RelA subunit phosphorylation, thus inhibiting NF-kappaB induced nuclear translocation ... Moreover, guanosine abrogated IFN-gamma induced phosphorylation on Ser ( 727 ) and translocation of STAT-1alpha to the nucleus as well as TNF-alpha-/Abeta induced IkappaBalpha and NF-kappaB p65/RelA subunit phosphorylation, thus inhibiting NF-kappaB induced nuclear translocation ... Guanosine effects were mediated by an increased phosphorylation of Akt, a PI3K downstream effector, as well as of ERK1/2 and p38 in the MAPK system, because culture pretreatment with selective ERK1/2, p38 MAPK, and PI3K antagonists ( U0126, SB203580, or LY294002, respectively ) counteracted guanosine inhibition on IFN-gamma/TNF-alpha induced CD40 expression and function as well as on STAT-1alpha or NF-kappaB nuclear translocation ... Guanosine effects were mediated by an increased phosphorylation of Akt, a PI3K downstream effector, as well as of ERK1/2 and p38 in the MAPK system, because culture pretreatment with selective ERK1/2, p38 MAPK, and PI3K antagonists ( U0126, SB203580, or LY294002, respectively ) counteracted guanosine inhibition on IFN-gamma/TNF-alpha induced CD40 expression and function as well as on STAT-1alpha or NF-kappaB nuclear translocation
Jost et al., J Immunol 2007 : Bcl10/Malt1 signaling is essential for TCR induced NF-kappaB activation in thymocytes but dispensable for positive or negative selection ... Bcl10/Malt1 signaling is essential for TCR induced NF-kappaB activation in thymocytes but dispensable for positive or negative selection
Doller et al., J Am Soc Nephrol 2007 (Disease Progression...) : It is interesting that only the JNK inhibitor SP600125 impaired the cytokine triggered MMP-9 level, suggesting that CsA, via inhibition of the JNK pathway, negatively interferes with the NF-kappaB dependent transcriptional control of MMP-9
Nai et al., JPEN J Parenter Enteral Nutr 2007 (Adenocarcinoma...) : Administration of PDTC dose dependently inhibited the NFkappaB activation in tumor tissues, inhibited IL-6 synthesis of the tumor cells, and attenuated the wasting of carcass weight, gastrocnemius muscle, and epididymal fat ... These results suggest that PDTC, an inhibitor of NFkappaB, can attenuate the development of cachexia in colon 26 tumor bearing mice through inhibition of IL-6 synthesis regulated by NFkappaB
Kaushal et al., J Neurosci 2007 (Neurodegenerative Diseases) : Microglia activation involved the signaling pathways p38 mitogen activated protein kinase ( MAPK ) and nuclear factor kappaB (NF-kappaB), which are important for upregulation of numerous proinflammatory molecules, and the KCa3.1 channels were functionally linked to activation of p38 MAPK but not NF-kappaB
Yun et al., Biol Pharm Bull 2007 : Furthermore, styraxoside A inhibited the LPS induced DNA binding activity of nuclear factor-kappaB (NF-kappaB)
Mathew et al., Am J Respir Cell Mol Biol 2007 : Macrophage activation was determined by measuring surface expression of CD11b, activation of NF-kappaB , phagocytic activity and production of reactive oxygen species, and cyclooxygenase (COX)-2 gene expression and production of prostaglandins ... Macrophage activation was determined by measuring surface expression of CD11b, activation of NF-kappaB , phagocytic activity and production of reactive oxygen species, and cyclooxygenase (COX)-2 gene expression and production of prostaglandins
Minsavage et al., J Pharmacol Exp Ther 2007 : Thus, disruption of p53/p90RSK mediated NF-kappaB signaling and activation of ARE/EpRE pathways may be effective strategies to delineate mechanisms of action of BFA induced inflammation and cell death signaling in immortalized versus normal skin systems ... Thus, disruption of p53/p90RSK mediated NF-kappaB signaling and activation of ARE/EpRE pathways may be effective strategies to delineate mechanisms of action of BFA induced inflammation and cell death signaling in immortalized versus normal skin systems
Zhang et al., PloS one 2006 : Both phenotypes are rescued by injection of RNA encoding the anti-apoptotic protein Bcl-xL ; Bcl-xL 's effects are dependent upon IkappaB kinase mediated activation of the bipartite transcription factor NF-kappaB
Frick et al., Inflamm Bowel Dis 2007 (Inflammation...) : Exposure of human epithelial cells to L. fermentum does not induce NF-kappaB activation and subsequent IL-8 secretion in HeLa cells, whereas Y. enterocolitica induces NF-kappaB activation and high levels of IL-8
Wild et al., World J Gastroenterol 2007 (Inflammation...) : Dietary intervention with omega-3 polyunsaturated fatty acids contained in fish oil may be useful for the care of IBD patients, and recent studies have stressed the role of PPAR on NFkappaB activity on the potential beneficial effect of dietary lipids on intestinal function
Bai et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2007 (Inflammation) : Overexpression of GILZ inhibits NF-KappaB and AP-1 activities, suggesting that GILZ possesses anti-inflammatory function
Park et al., Eur J Pharmacol 2007 : Furthermore, siegeskaurolic acid inhibited the nuclear factor-kappaB (NF-kappaB) activation induced by LPS , and this was associated with the prevention of inhibitor kappaB degradation ( I kappaB ), and subsequently with decreased nuclear p65 and p50 protein levels
Henriquet et al., Lung Cancer 2007 (Adenocarcinoma...) : Conversely, TPA inhibited TNF-alpha induced NF-kappaB signaling and was a weak activator of this pathway ... Thus, TPA did not sufficiently activate NF-kappaB to increase transcription through the low affinity NF-kappaB binding sites on RANTES promoter and its inhibitory effect on TNF-alpha induced NF-kappaB signaling resulted in a reduced transcription rate
Eddleston et al., J Allergy Clin Immunol 2007 : Overexpression of GILZ in BEAS-2B cells significantly inhibited the ability of IL-1beta, LPS, and polyinosinic : polycytidylic acid to activate NF-kappaB , whereas knockdown of GILZ inhibited the ability of dexamethasone to suppress IL-1beta induced chemokine expression
Sunday et al., Am J Physiol Heart Circ Physiol 2007 (Vasculitis, Central Nervous System) : Estrogen significantly inhibited the LPS induced increase in NF-kappaB DNA binding activity in cerebral vessels from animals at both ages
Ranjith-Kumar et al., J Biol Chem 2007 : We also made over 80 mutations in the residues that could affect these features in the full-length TLR3 and examined their effects in TLR3 mediated NF-kappaB activation
Fraser et al., J Biol Chem 2007 : In antibody supershift experiments, anti-p50 antibody supershifted the C1q induced NFkappaB complex, whereas anti-p65 antibody had little effect, suggesting that C1q induced the translocation of NFkappaB p50p50 homodimers
Kim et al., Free Radic Biol Med 2007 : Thus, we have demonstrated, for the first time, that the interaction of DDR1 with collagen induces iNOS expression and subsequent NO synthesis in J774 cells through activation of NF-kappaB , p38 MAPK, and JNK and suggest that intervention of DDR1 signaling in macrophages may be useful in controlling inflammatory diseases in which NO plays a critical role
Gray-Schopfer et al., Cancer Res 2007 (MAP Kinase Signaling System...) : The survival mechanism requires nuclear factor-kappaB (NF-kappaB) transcription factor activity, which is strongly induced by TNF-alpha in these cells
Lemmers et al., J Biol Chem 2007 (Rhabdoviridae Infections) : Essential role for caspase-8 in Toll-like receptors and NFkappaB signaling ... In response to TLR4 stimulation, caspase-8 is recruited to a complex containing IKKalphabeta, and its loss resulted in delayed NFkappaB nuclear translocation and impaired NFkappaB transcriptional activity
Sun et al., Oncogene 2007 (Prostatic Neoplasms) : Additionally, overexpression of NF-kappaB partly reversed the ability of wortmannin to inhibit HIF-1alpha dependent transcriptional activity, suggesting that NF-kappaB contributes to Akt mediated HIF-1alpha accumulation during hypoxia
Valdés et al., Am J Physiol Cell Physiol 2007 (Calcium Signaling) : NF-kappaB activation is mediated by IkappaBalpha degradation and p65 translocation to the nucleus ... NF-kappaB activation is mediated by IkappaBalpha degradation and p65 translocation to the nucleus
Armstrong et al., Neoplasia (New York, N.Y.) 2006 (Neuroblastoma) : Moreover, p53 is necessary for NF-kappaB activation because cells with inactive p53 were resistant to NF-kappaB mediated cell death
Ou et al., Arch Toxicol 2007 : Furthermore, several apoptotic signaling pathways which showed NF-kappaB activation, increased cytosolic calcium, alteration of mitochondrial membrane potential, cytochrome c release and activation of caspase 3 were also investigated
Ckless et al., Am J Respir Cell Mol Biol 2007 : Our results demonstrate that reduction of arginase activity enhanced cellular content of NO and S-nitrosated proteins, and resulted in decreases in TNF-alpha- or LPS stimulated NF-kappaB DNA binding and transcriptional activity, in association with enhanced S-nitrosation of p50 ... Our results demonstrate that reduction of arginase activity enhanced cellular content of NO and S-nitrosated proteins, and resulted in decreases in TNF-alpha- or LPS stimulated NF-kappaB DNA binding and transcriptional activity, in association with enhanced S-nitrosation of p50 ... Conversely, overexpression of arginase I decreased cellular S-nitrosothiol content and enhanced IkappaB kinase activity and NF-kappaB DNA binding, and decreased S-nitrosation of p50
Delaney et al., Cell cycle (Georgetown, Tex.) 2006 : For example, NFkappaB signaling in a particular cell may or may not require TAK1 depending on the nature of the activating signal
Karmakar et al., Apoptosis 2007 (Neuroblastoma) : Apoptosis was associated with an increase in [ Ca ( 2+ ) ] ( i ), increase in Bax : Bcl-2 ratio, mitochondrial release of cytochrome c, increase in cytosolic Smac/Diablo, and down regulation of inhibitor-of-apoptosis proteins and nuclear factor kappa B (NFkappaB)
Ivory et al., Eur J Immunol 2007 (Entamoebiasis) : The activation of DC by Gal-lectin was mediated by MAPK and NF-kappaB
Sharif et al., BMC immunology 2007 : Transcriptional profiling of the LPS induced NF-kappaB response in macrophages
Qi et al., Virology 2007 : Furthermore, forced expression of a super-repressor form of IkappaBalpha or pretreatment with ritonavir efficiently blocked the activation of NF-kappaB and HIV-1 replication in J ( 22 ) -HL-60 cells co-cultured with MOLT-4 T cells
Liuzzo et al., J Am Coll Cardiol 2007 (Angina, Unstable) : C-reactive protein induced a significant but modest in vitro NF-kappaB activation in human monocytes ( p = 0.002 )
Duxbury et al., Biochem Biophys Res Commun 2007 (Adenocarcinoma...) : RRM2 induces NF-kappaB dependent MMP-9 activation and enhances cellular invasiveness ... RRM2 induces NF-kappaB dependent MMP-9 activation and enhances cellular invasiveness ... RRM2 overexpression increases pancreatic adenocarcinoma cellular invasiveness and MMP-9 expression in a NF-kappaB dependent manner ... RRM2 overexpression increases pancreatic adenocarcinoma cellular invasiveness and MMP-9 expression in a NF-kappaB dependent manner
Liu et al., Mol Immunol 2007 : Further studies show that the triptolide mediated inhibitory effects of LPS induced activation of phosphatidylinositol-3 kinase (PI3-K)/Akt and nuclear NF-kappaB activation are involved in down-regulation of COX-2 and CCR7 expression resulting in impaired migration to secondary lymphoid organs of DC ... Further studies show that the triptolide mediated inhibitory effects of LPS induced activation of phosphatidylinositol-3 kinase (PI3-K)/Akt and nuclear NF-kappaB activation are involved in down-regulation of COX-2 and CCR7 expression resulting in impaired migration to secondary lymphoid organs of DC ... Further studies show that the triptolide mediated inhibitory effects of LPS induced activation of phosphatidylinositol-3 kinase (PI3-K)/Akt and nuclear NF-kappaB activation are involved in down-regulation of COX-2 and CCR7 expression resulting in impaired migration to secondary lymphoid organs of DC ... Further studies show that the triptolide mediated inhibitory effects of LPS induced activation of phosphatidylinositol-3 kinase (PI3-K)/Akt and nuclear NF-kappaB activation are involved in down-regulation of COX-2 and CCR7 expression resulting in impaired migration to secondary lymphoid organs of DC ... Further studies show that the triptolide mediated inhibitory effects of LPS induced activation of phosphatidylinositol-3 kinase (PI3-K)/Akt and nuclear NF-kappaB activation are involved in down-regulation of COX-2 and CCR7 expression resulting in impaired migration to secondary lymphoid organs of DC
Paris et al., Neurosci Lett 2007 (Alzheimer Disease...) : In the present study, we investigated the effect of various NF-kappaB inhibitors on the production of Abeta1-40, Abeta1-42, secreted APP ( sAPPbeta and sAPPalpha ) and APP C-terminal fragments (APP-CTF) using CHO cells overexpressing the beta-amyloid precursor protein (APP)
Samant et al., Molecular cancer 2007 (Breast Neoplasms) : Breast cancer metastasis suppressor 1 ( BRMS1 ) inhibits osteopontin transcription by abrogating NF-kappaB activation ... Our results show that BRMS1 regulates OPN transcription by abrogating NF-kappaB activation ... Our results show that BRMS1 regulates OPN transcription by abrogating NF-kappaB activation
Shimizu et al., Mol Hum Reprod 2007 : Activation of NF-kB by LPS was inhibited by treatment with C3 exoenzyme, Y-27632 or RhoA siRNA ... However, inhibition of the RhoA/Rho-kinase pathway did not attenuate the activation of NF-kB by IL-1beta
Witowski et al., Kidney Int 2007 : The novel observation was that IL-17A stimulated NF-kappaB activation in HPMCs followed a biphasic profile, with an early induction ( 45 min ), followed by the return to basal levels ( 90 min ), and a delayed induction ( 3 h )
Kang et al., Pharmacol Res 2007 : Furthermore, EAAD suppressed LPS induced phosphorylation of p38 MAPK and extracellular-signal regulated kinases 1/2 ( ERK1/2 ), I-kappaBalpha degradation, and NF-kappaB activation in RAW 264.7 cells ... These results suggest that EAAD has the inhibitory effects on LPS induced TNF-alpha, NO and PGE ( 2 ) production, and expression of iNOS and COX-2 in macrophage through blockade in the phosphorylation of MAPKs, following I-kappaBalpha degradation and NF-kappaB activation
Chung et al., Carcinogenesis 2007 (Acute Disease...) : When mouse skin was treated after TPA induced production of papillomas, xanthorrhizol remarkably suppressed the expression of ODC, iNOS and COX-2 and inhibited the activation of NF-kappaB ... When mouse skin was treated after TPA induced production of papillomas, xanthorrhizol remarkably suppressed the expression of ODC , iNOS and COX-2 and inhibited the activation of NF-kappaB ... When mouse skin was treated after TPA induced production of papillomas, xanthorrhizol remarkably suppressed the expression of ODC, iNOS and COX-2 and inhibited the activation of NF-kappaB ... Taken together, the present study demonstrates that xanthorrhizol not only delays or inhibits tumor formation, but also reverses the carcinogenic process at pre-malignant stages by reducing the protein levels of ODC, iNOS and COX-2 regulated by the NF-kappaB , mitogen activated protein kinases and/or Akt ... Taken together, the present study demonstrates that xanthorrhizol not only delays or inhibits tumor formation, but also reverses the carcinogenic process at pre-malignant stages by reducing the protein levels of ODC , iNOS and COX-2 regulated by the NF-kappaB , mitogen activated protein kinases and/or Akt ... Taken together, the present study demonstrates that xanthorrhizol not only delays or inhibits tumor formation, but also reverses the carcinogenic process at pre-malignant stages by reducing the protein levels of ODC, iNOS and COX-2 regulated by the NF-kappaB , mitogen activated protein kinases and/or Akt
Zhande et al., Am J Physiol Heart Circ Physiol 2007 : EPO caused a prolonged activation of nuclear factor (NF)-kappaB , which was blocked by inhibition of PI3K, but not by inhibition of mitogen activated protein ( MAP ) /ERK kinase ( MEK ), suggesting that EPO activated NF-kappaB requires PI3K activity ... EPO caused a prolonged activation of nuclear factor (NF)-kappaB , which was blocked by inhibition of PI3K , but not by inhibition of mitogen activated protein ( MAP ) /ERK kinase ( MEK ), suggesting that EPO activated NF-kappaB requires PI3K activity
An et al., Mol Cancer Ther 2007 (Carcinoma, Renal Cell...) : Using pharmacologic and genetic approaches, we also showed that EGFR mediated NF-kappaB activation occurs through the phosphotidylinositol-3-OH kinase/AKT pathway
Lich et al., J Immunol 2007 (Inflammation) : In this report, we show that Monarch-1 inhibits CD40 mediated activation of NF-kappaB via the non-canonical pathway in human monocytes ... In this report, we show that Monarch-1 inhibits CD40 mediated activation of NF-kappaB via the non-canonical pathway in human monocytes ... This inhibition stems from the ability of Monarch-1 to associate with and induce proteasome mediated degradation of NF-kappaB inducing kinase
Moschen et al., J Immunol 2007 (Inflammation...) : Visfatin induced effects involve p38 as well as MEK1 pathways as determined by inhibition with MAPK inhibitors and we observed activation of NF-kappaB
Mukherjee et al., J Immunol 2007 : This study further addressed that IkappaB kinase (IKK) , a phosphorylation dependent regulator of NF-kappaB , plays an important regulatory role in the TNF-alpha mediated induction of the inflammatory cell surface molecule ICAM-1
Azuma et al., Biochem Biophys Res Commun 2007 : Although the functional mechanism whereby extra-cellular BPI modulates the intra-cellular signal pathways selected by the TLR adaptors, MyD88 and TICAM-1 ( TRIF ), remains unknown, we infer that the lipid A portion of LPS participates in LBP amplified IFN-beta induction and that BPI binding to LPS leads to inhibition of the activation of NF-kappaB and IFN-beta by LPS or agonistic lipid A via TLR4 in an extrinsic mode ... Although the functional mechanism whereby extra-cellular BPI modulates the intra-cellular signal pathways selected by the TLR adaptors, MyD88 and TICAM-1 ( TRIF ), remains unknown, we infer that the lipid A portion of LPS participates in LBP amplified IFN-beta induction and that BPI binding to LPS leads to inhibition of the activation of NF-kappaB and IFN-beta by LPS or agonistic lipid A via TLR4 in an extrinsic mode
Yotsui et al., FEBS Lett 2007 (Atherosclerosis) : Nuclear factor kappaB (NF-kappaB) activity was enhanced by IL-1beta and reduced by aspirin, indicating that decreased ICAM-1 and VCAM-1 expression was due to reduced NF-kappaB activity.Thus, aspirin inhibits the adhesion of Jurkat T cells to IL-1beta activated AoSMC by reducing NF-kappaB activity and decreasing expression of ICAM-1 and VCAM-1, and may prevent the development of atherosclerosis
Lee et al., Food Chem Toxicol 2007 (Colitis) : In addition, the results obtained from electrophoretic mobility shift assay ( EMSA ) and western blot analysis showed that UDN glycoprotein dose-dependently inhibits DNA binding activity of nuclear factor-kappa B (NF-kappaB) , and activities of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and manganese-superoxide dismutases ( Mn-SOD ) in LPS stimulated RAW 264.7 cells
Yu et al., Hypertension 2007 (Disease Models, Animal...) : We investigated the role of nuclear factor kappaB (NF-kappaB) in the cytokine mediated induction of cyclooxygenase-2 activity in the paraventricular nucleus of hypothalamus ( PVN ), a critical cardiovascular and autonomic center, in rats with heart failure ( HF )
Rhee et al., J Biochem Mol Biol 2007 : Chromatin immunoprecipitation ( ChIP ) assays showed that functional interaction between NF-kappaB and the MMP-9 promoter element is necessary for LPS activated MMP-9 induction in RAW 264.7 cells ... In conclusion, our observations demonstrate that NF-kappaB contributes to LPS induced MMP-9 gene expression in a mouse macrophage cell line
Zaheer et al., J Neurochem 2007 (Encephalitis...) : Inhibition of p38 MAPK or NF-kappaB by specific inhibitors prevented GM-CSF production
Gordon et al., J Pathol 2007 (Mesothelioma...) : Co-incubation of MPM cells with TNF-alpha and pyrrolidine dithiocarbamate ( PDTC ), an NF-kappaB inhibitor , prevented TNF mediated up-regulation of IAP gene expression levels
Sosne et al., Exp Eye Res 2007 (Keratitis) : The purpose of this study was to determine the effect of thymosin beta 4 (Tbeta4) on NFkappaB protein levels, activation, phosphorylation, and nuclear translocation in a model of tumor necrosis factor (TNF)-alpha mediated corneal inflammation ... In contrast, Tbeta4 blocked nuclear translocation of the NFkappaB p65 subunit in TNF-alpha stimulated corneal epithelial cells ... Tbeta4 , a protein with diverse cellular functions including wound healing and suppression of inflammation, inhibits the activation of NFkappaB in TNF-alpha stimulated cells
Kanda et al., Endocrinology 2007 (MAP Kinase Signaling System...) : Inhibitors of Janus kinase ( JAK ), p38 MAPK, and MAPK/ERK kinase (MEK) suppressed prolactin- plus IFN-gamma induced CXCL9, CXCL10, and CXCL11 production and NF-kappaB , STAT1, and IRF-1 activities ... Inhibitors of Janus kinase ( JAK ) , p38 MAPK, and MAPK/ERK kinase (MEK) suppressed prolactin- plus IFN-gamma induced CXCL9, CXCL10, and CXCL11 production and NF-kappaB , STAT1, and IRF-1 activities
Schmidt et al., EMBO J 2007 (Neoplasms...) : Critical role for NF-kappaB induced JunB in VEGF regulation and tumor angiogenesis
Otkjaer et al., J Invest Dermatol 2007 (MAP Kinase Signaling System) : IL-20 is assumed to be a key cytokine in the pathogenesis of psoriasis and possibly cancer, and therefore the p38 MAPK, MSK1, and NF-kappaB may be important new molecular targets for the modulation of IL-20 expression in these diseases
Lewis et al., Arch Dermatol Res 2007 : These experiments indicate that although both TNFalpha and UVB stimulate NF-kappaB DNA binding activity in normal human keratinocytes, the mechanisms of NF-kappaB activation by each stimulus is different ... In contrast to the NF-kappaB response following TNFalpha, activation of NF-kappaB by UVB is independent of Ikappa Balpha degradation
Lehrke et al., J Am Coll Cardiol 2007 (Acute Disease...) : Endotoxin induction of CXCL16 in human macrophages was attenuated by aspirin, nuclear factor (NF)-kappa-B inhibition and peroxisome proliferator activated receptor ( PPAR)-gamma agonists
Wang et al., J Biomed Sci 2007 : Expression of A20 has been shown to protect from TNF induced apoptosis and also functions via a negative-feedback loop to block NF-kappaB activation induced by TNF and other stimuli
Nogalska et al., Exp Neurol 2007 : Accordingly, interfering with pathways inducing ER stress, NF-kappaB activation or its action on the MstnPP gene promoter might prevent Mstn increase and provide a new therapeutic approach for s-IBM and, possibly, for muscle atrophy in other neuromuscular diseases
Crispen et al., Prostate 2007 (Neoplasm Invasiveness...) : Treatment with alpha-tocopherol succinate ( VES ) inhibits NF-kappaB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen independent PC-3, DU-145, and CA-HPV-10 cells ... Treatment with alpha-tocopherol succinate ( VES ) inhibits NF-kappaB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen independent PC-3, DU-145, and CA-HPV-10 cells ... Treatment with alpha-tocopherol succinate ( VES ) inhibits NF-kappaB but augments AP-1 activity, reduces expression of IL-6, IL-8, and VEGF, suppresses cell adhesion, ICAM-1 and gp130 expression in androgen independent PC-3, DU-145, and CA-HPV-10 cells
Larsen et al., Diabetologia 2007 : Nuclear factor kappa B (NFkappaB) is a critical signalling molecule in inflammation and is required for expression of the gene encoding inducible NO synthase (iNOS) and of pro-apoptotic genes
Li et al., J Cell Biochem 2007 (Cardiomegaly...) : Furthermore, long-term AMPK activation attenuated protein synthesis, diminished calcineurin-nuclear factor of activated T cells ( NFAT ) and nuclear factor kappaB (NF-kappaB) signaling in pressure overload induced hypertrophic hearts ... Our in vitro experiments further proved that activation of AMPK by infection of AdAMPK blocked cardiac hypertrophy and NFAT, NF-kappaB , and MAPK signal pathways
Li et al., J Bone Miner Res 2007 (Osteoporosis) : The effect of TNFalpha induced NF-kappaB signaling on Smad signaling and on in vitro osteoblast mineralization was examined using specific NF-kappaB inhibitors and activators, and effects of TNFalpha induced NF-kappaB signaling on BMP-2 induced Runx2 mRNA were examined using RT-PCR ... The effect of TNFalpha induced NF-kappaB signaling on Smad signaling and on in vitro osteoblast mineralization was examined using specific NF-kappaB inhibitors and activators, and effects of TNFalpha induced NF-kappaB signaling on BMP-2 induced Runx2 mRNA were examined using RT-PCR
Cohen-Lahav et al., J Steroid Biochem Mol Biol 2007 : The present results demonstrate that 1,25 ( OH ) ( 2 ) D ( 3 ) and 1,24 ( OH ) ( 2 ) D ( 2 ) inhibit TNFalpha expression in macrophages, by increasing IkappaBalpha and decreasing NFkappaB activity
Ishinaga et al., EMBO J 2007 (Haemophilus Infections) : Here, we show that TGF-beta1 induces acetylation of NF-kappaB p65 subunit to synergistically enhance bacterium nontypeable Haemophilus influenzae induced NF-kappaB activation and inflammatory response in vitro and in vivo ... Acetylation of p65 at lysine 221 by TGF-beta1 is critical for synergistic enhancement of bacteria induced DNA binding activity, NF-kappaB activation, NF-kappaB dependent transcription of TNF-alpha and IL-1beta and interstitial polymorphonuclear neutrophil infiltration in vitro and in vivo ... Acetylation of p65 at lysine 221 by TGF-beta1 is critical for synergistic enhancement of bacteria induced DNA binding activity, NF-kappaB activation, NF-kappaB dependent transcription of TNF-alpha and IL-1beta and interstitial polymorphonuclear neutrophil infiltration in vitro and in vivo
Chang et al., Carcinogenesis 2007 : Second, E ( 2 ) potentiated BaP induced nuclear factor-kappaB (NF-kappaB) activation, which regulates COX-2 expression
Saadane et al., Am J Respir Cell Mol Biol 2007 (Cystic Fibrosis...) : This was also associated with inhibition of IkappaBalpha degradation and NF-kappaB activation
Kanda et al., Eur J Immunol 2007 : Inhibitors of PI3 K, p38 MAPK , and MEK suppressed IL-18 plus IFN-gamma induced CXCL9, CXCL10, and CXCL11 production and NF-kappaB , STAT1, and IRF-1 activities ... Inhibitors of PI3 K , p38 MAPK, and MEK suppressed IL-18 plus IFN-gamma induced CXCL9, CXCL10, and CXCL11 production and NF-kappaB , STAT1, and IRF-1 activities
Manjarrez-Orduño et al., Eur J Immunol 2007 : The cross linking of the surface molecule CD38 induces calcium mobilization, protein phosphorylation and NF-kappaB translocation into the nucleus, ultimately leading to proliferation and isotype switching toward IgG1
van Etten et al., Eur J Immunol 2007 : In transfection experiments, the presence of the shorter F-VDR resulted in higher NF-kappaB- and NFAT-driven transcription as well as higher IL-12p40 promoter-driven transcription
Iwasaki et al., Atherosclerosis 2008 (Hyperglycemia) : High glucose induces plasminogen activator inhibitor-1 expression through Rho/Rho-kinase mediated NF-kappaB activation in bovine aortic endothelial cells ... High glucose induces plasminogen activator inhibitor-1 expression through Rho/Rho-kinase mediated NF-kappaB activation in bovine aortic endothelial cells ... NF-kappaB activity determined using the luciferase reporter gene assay was significantly enhanced by high glucose, and pretreatment with Y-27632 inhibited high glucose induced PAI-1 expression at the basal level ... An inhibitor of NF-kappaB action, namely parthenolide ( 0.1 microM ), BAY 11-7082 ( 5 microM ) and SN50 ( 1 microM ), significantly blocked high glucose mediated PAI-1 expression to a level with low glucose ( 5.7 mM ) ... These data suggested that high glucose induced PAI-1 expression in endothelial cells is mediated by NF-kappaB activation through the Rho/Rho-kinase pathway
Tao et al., J Surg Res 2007 (Crohn Disease) : The IL-1beta induced ICAM-1 was also inhibited by a potent inhibitor of NF-kappaB , MG132
Kumar et al., Biochem Pharmacol 2007 : We demonstrate that ILTG inhibits the translocation and activation of nuclear factor-kappaB (NF-kappaB) by blocking the phosphorylation and subsequent degradation of IkappaBalpha
Razeghi et al., Cardiovasc Res 2007 : Lack of NF-kappaB1 ( p105/p50 ) attenuates unloading induced downregulation of PPARalpha and PPARalpha regulated gene expression in rodent heart
Fitzgerald et al., Vet Immunol Immunopathol 2007 (Mastitis, Bovine) : Tumour necrosis factor-alpha (TNF-alpha) increases nuclear factor kappaB (NFkappaB) activity in and interleukin-8 (IL-8) release from bovine mammary epithelial cells ... TNF-alpha enhanced NFkappaB activity in bovine mammary epithelial monolayers ... These data support a role for TNF-alpha in activation of NFkappaB and release of IL-8 from bovine mammary epithelial cells
Yanagisawa et al., Biochim Biophys Acta 2007 (Melanoma) : Conversely, DGKalpha-knockdown considerably inhibited the NF-kappaB activity
Wu et al., Int Immunopharmacol 2007 : These findings suggest that the inhibition of LPS induced NO formation and TNF-alpha production in microglia by ginsenosides is due to its inhibition of NF-kappaB , which may be the mechanistic basis for the anti-inflammatory effects of ginsenosides
Nizamutdinova et al., Int Immunopharmacol 2007 (Atherosclerosis) : The inhibitory effect of paeonol on ICAM-1 production might be mediated by inhibiting p38, ERK and NF-kappaB signaling pathways, which are involved in TNF-alpha induced ICAM-1 production
Ahn et al., J Immunol 2007 (Neoplasms) : We found that simvastatin inhibited TNF-alpha induced NF-kappaB activation, and l-mevalonate reversed the suppressive effect, indicating the role of hydroxy-3-methylglutaryl-CoA reductase
Zhang et al., J Immunol 2007 : Inflammatory cytokine overexpression resulted from an increased activation of NF-kappaB and the MAPK signaling cascade and a reduced activation of the PI3K-Akt pathway following LPS stimulation
Hamazaki et al., Nat Immunol 2007 : We provide evidence that such Cld3,4 ( hi ) UEA-1 ( + ) TECs represented the initial progenitors specified for Aire ( + ) mTECs, whose development crucially required NF-kappaB inducing kinase and the adaptor molecule TRAF6
Kammanadiminti et al., Infect Immun 2007 : SAP induced phosphorylation of p65 and enhanced NF-kappaB transcriptional activity, which are phosphatidylinositol 3-kinase ( PI3 kinase ) dependent
Yamagishi et al., Ophthalmic Res 2007 (Diabetes Mellitus, Experimental...) : In addition, PEDF completely inhibited superoxide generation and NF-kappaB activation in AGE exposed endothelial cells ... These results demonstrated that PEDF could inhibit diabetes- or AGE induced RAGE gene expression by blocking the superoxide mediated NF-kappaB activation
Noels et al., J Biol Chem 2007 (Lymphoma, B-Cell, Marginal Zone...) : In contrast, TRAF6 was essential for NF-kappaB activation by all fusion variants, and we identified a novel TRAF6 binding site in the second immunoglobulin domain of MALT1, which enhanced NF-kappaB activation when present in the fusion protein ... Finally, binding of TRAF2 to BIR1 contributed to NF-kappaB activation by API2.MALT1, although additional mechanisms involving BIR1 mediated raft association are also important ... Finally, binding of TRAF2 to BIR1 contributed to NF-kappaB activation by API2.MALT1 , although additional mechanisms involving BIR1 mediated raft association are also important ... Finally, binding of TRAF2 to BIR1 contributed to NF-kappaB activation by API2.MALT1, although additional mechanisms involving BIR1 mediated raft association are also important ... Finally, binding of TRAF2 to BIR1 contributed to NF-kappaB activation by API2.MALT1 , although additional mechanisms involving BIR1 mediated raft association are also important
Takahashi et al., Atherosclerosis 2007 (Hypertrophy) : An electrophoretic mobility shift assay showed that Ang II increased the TRPC1 promoter 's NF-kappaB binding activity ... In conclusion, our data suggest that Ang II and subsequent NF-kappaB activation induces hCASMC hypertrophy through an enhancement of TRPC1 expression
Zhi et al., J Leukoc Biol 2007 (Inflammation) : We also found that H2S stimulated the phosphorylation and activation of ERK1/2, but not of p38 MAPK and JNK, and pretreatment with PD98059, a selective MEK1 antagonist, could inhibit H2S induced NF-kappaB activation markedly ... We also found that H2S stimulated the phosphorylation and activation of ERK1/2 , but not of p38 MAPK and JNK, and pretreatment with PD98059, a selective MEK1 antagonist, could inhibit H2S induced NF-kappaB activation markedly
Deeb et al., J Pharmacol Exp Ther 2007 (Prostatic Neoplasms) : Furthermore, overexpression of constitutively active Akt1 in cancer cells prevented the inhibition of NF-kappaB by curcumin ... Because expression of antiapoptotic Bcl-2, Bcl-xL , and X-chromosome linked inhibitor of apoptosis protein ( XIAP ) is regulated by NF-kappaB , both curcumin and SH-6 decreased the levels of these proteins in PC3 cells through inhibition of NF-kappaB ... Because expression of antiapoptotic Bcl-2, Bcl-xL, and X-chromosome linked inhibitor of apoptosis protein ( XIAP ) is regulated by NF-kappaB , both curcumin and SH-6 decreased the levels of these proteins in PC3 cells through inhibition of NF-kappaB ... Because expression of antiapoptotic Bcl-2 , Bcl-xL, and X-chromosome linked inhibitor of apoptosis protein ( XIAP ) is regulated by NF-kappaB , both curcumin and SH-6 decreased the levels of these proteins in PC3 cells through inhibition of NF-kappaB
Shakibaei et al., Biochem Pharmacol 2007 (Osteoarthritis) : Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes : Implications for the treatment of osteoarthritis ... Treatment of chondrocytes with curcumin suppressed IL-1beta induced NF-kappaB activation via inhibition of IkappaBalpha phosphorylation, IkappaBalpha degradation, p65 phosphorylation and p65 nuclear translocation
Raghav et al., Eur J Pharmacol 2007 (Disease Models, Animal) : Electrophoretic mobility shift assay ( EMSA ) and Western blot analyses indicated that the plant extract and the isolated active compound blocked the LPS induced activation of NF-kappaB through the prevention of inhibitor-kB ( IkB ) degradation
Theroux et al., Brain Res Bull 2007 (Olfaction Disorders) : Raf kinase inhibitory protein ( RKIP-1 ) is involved in the regulation of the MAP kinase, NF-kappaB , and GPCR signaling pathways
Luedde et al., Cancer Cell 2007 (Carcinoma, Hepatocellular...) : The IkappaB kinase (IKK) subunit NEMO/IKKgamma is essential for activation of the transcription factor NF-kappaB , which regulates cellular responses to inflammation ... These results reveal that NEMO mediated NF-kappaB activation in hepatocytes has an essential physiological function to prevent the spontaneous development of steatohepatitis and hepatocellular carcinoma, identifying NEMO as a tumor suppressor in the liver
Law et al., Mol Cell Biol 1992 : Neither MTA nor mevinolin significantly inhibited NF-kappa B activation by phorbol myristate acetate, suggesting that these agents act early in signal transduction
Shamshiev et al., J Exp Med 2007 (Dyslipidemias...) : We found that oxidized low-density lipoprotein ( oxLDL ) was the key active component responsible for this effect, as it could directly uncouple TLR mediated signaling on CD8alpha ( - ) myeloid DCs and inhibit NF-kappaB nuclear translocation
Rigby et al., Oncogene 2007 (Colonic Neoplasms...) : In vitro, SOCS3 overexpression reduced proliferation, IL-6 mediated STAT3 activation and tumor necrosis factor (TNF) alpha mediated NF-kappaB activation ... In vitro, SOCS3 overexpression reduced proliferation, IL-6 mediated STAT3 activation and tumor necrosis factor (TNF) alpha mediated NF-kappaB activation
Fan et al., Oncogene 2007 : Ras effector pathways modulate scatter factor stimulated NF-kappaB signaling and protection against DNA damage ... Further studies revealed that Raf1 positively regulates the ability of SF to stimulate NF-kappaB activity and cell protection ... The ability of Raf1 to stimulate NF-kappaB activity was not due to the classical Raf1 -- > MEK1/2 -- > ERK1/2 pathway ... In contrast, RalA , a target of the Ras/RalGDS pathway negatively regulated the ability of SF to stimulate NF-kappaB activity and cell protection
Vendrell et al., Oncogene 2007 (Breast Neoplasms) : The zinc-finger protein A20/TNFAIP3 , an inhibitor of nuclear factor-kappaB (NF-kappaB) activation, has been shown to protect MCF-7 breast carcinoma cells from TNFalpha induced apoptosis
Zhang et al., Chin J Cancer 2007 (Cell Transformation, Neoplastic...) : When pLNSX-LMP1 and pBabe-IkappaB were cotransfected at a ratio of 1 : 1, IkappaBalpha inhibited LMP1 mediated NF-kappaB activation by 75 % û and at a ratio of 3 : 1, it almost completely inhibited LMP1 mediated NF-kappaB activation ... When pLNSX-LMP1 and pBabe-IkappaB were cotransfected at a ratio of 1 : 1, IkappaBalpha inhibited LMP1 mediated NF-kappaB activation by 75 % û and at a ratio of 3 : 1, it almost completely inhibited LMP1 mediated NF-kappaB activation
Szeto et al., J Steroid Biochem Mol Biol 2007 : Involvement of the vitamin D receptor in the regulation of NF-kappaB activity in fibroblasts ... We have used mouse embryonic fibroblasts ( MEFs ) derived from VDR ( +/- ) and VDR ( -/- ) mice to determine whether the nuclear vitamin D receptor (VDR) is directly involved in the regulation of NF-kappaB activation ... These data suggest that VDR plays an inhibitory role in the regulation of NF-kappaB activation
Schumann et al., Proc Natl Acad Sci U S A 2007 : Up-regulation of FLIP enhanced NF-kappaB activity via NF-kappaB inducing kinase and RelB
Lin et al., J Cell Physiol 2007 (Lung Neoplasms) : In previous study, interleukin-1beta (IL-1beta) has been shown to induce ICAM-1 expression through MAPKs and NF-kappaB in A549 cells
Tammali et al., Cancer Lett 2007 (Colonic Neoplasms) : Inhibition of AR prevented TNF-alpha induced activation of PKC and NF-kappaB which resulted in the abrogation of Cox-2 mRNA and protein expression
Noonan et al., Recent Results Cancer Res 2007 (Cell Transformation, Neoplastic...) : NAC and the flavonoids all suppressed the IkB/NF-kappaB signaling pathway even in the presence of NF-kappaB stimulation by TNFalpha, and showed reduced expression of many NF-kappaB target genes
Lin et al., Cell Signal 2007 : Involvement of MAPKs and NF-kappaB in LPS induced VCAM-1 expression in human tracheal smooth muscle cells ... LPS stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha was blocked by helenalin, U0126, SB202190, or SP600125
Tsytsykova et al., J Biol Chem 2007 : We find that TNF mRNA production in response to ionophore is NF-kappaB independent, but inhibition of NF-kappaB activation attenuates virus- and LPS induced TNF mRNA levels after initial induction ... We find that TNF mRNA production in response to ionophore is NF-kappaB independent, but inhibition of NF-kappaB activation attenuates virus- and LPS induced TNF mRNA levels after initial induction
Zhao et al., J Biol Chem 2007 (Inflammation) : Lauric acid ( C12 : 0 ) dose dependently activated NF-kappaB and induced IL-8 expression in HCT116 cells, which express both Nod1 and Nod2, but not detectable amounts of TLR2 and TLR4
Lentsch et al., Shock 2007 (Acute Disease...) : In TK-/- mice, there was far less IkappaB-alpha and IkappaB-beta protein and greater activation of NF-kappaB
Doleschall et al., Dev Comp Immunol 2007 : The cloned bovine p65 was expressed in mammalian cells, and it induced the NF-kappaB-specific luciferase reporter gene expression
Hohmann et al., J Biol Chem 1992 (Leukemia, Promyelocytic, Acute) : NF-kappa B activation by phorbol 12-myristate 13-acetate ( PMA ) and interleukin-1 was inhibited by staurosporin ... Thus, induction of NF-kappa B and p51 mRNA by TNF-alpha was not mediated by a staurosporin-sensitive factor, but NF-kappa B activation by TNF-alpha was even reduced by action of a staurosporin-sensitive factor ... Decreased levels of phosphorylation of TNF-R alpha ( TNF receptor type alpha ) after staurosporin-treatment correlated with increased induction of NF-kappa B by TNF-alpha ... PMA induced disappearance of TNF-R alpha by shedding into the surrounding medium, with kinetics similar to those of its inhibition of NF-kappa B activation by TNF-alpha
Vincenti et al., J Cell Physiol 1992 : Prolonged exposure to phorbol ester, a treatment which down-regulates PKC, had no effect on LPS induction of NF-kappa B activity in these cells ... These results suggest that the induction of NF-kappa B in macrophages by LPS is independent of PKC
Bachmeier et al., Cell Physiol Biochem 2007 (Breast Neoplasms...) : 68 % of Curcumin treated but only 17 % of untreated animals showed no or very few lung metastases, most likely as a consequence of down-regulation of NFkappa B/AP-1 dependent MMP expression and direct apoptotic effects on circulating tumor cells but not on established metastases
Watanabe et al., Lab Invest 2007 (Hodgkin Disease) : We also present the data that indicate the involvement of IkappaBbeta in NF-kappaB induction by topoisomerase inhibitors ... The results also indicate that IkappaBbeta is involved in NF-kappaB activation in H-RS cells and IkappaBbeta substitutes for IkappaBalpha in H-RS cells lacking wild-type IkappaBalpha
Liang et al., J Cell Physiol 2007 (MAP Kinase Signaling System) : Furthermore, IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha was blocked by helenalin ... Furthermore, IL-1beta stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha was blocked by helenalin ... Finally, the reporter gene assay revealed that MAPKs and NF-kappaB are required for IL-1beta induced MMP-9 luciferase activity in HTSMCs. MMP-9 promoter activity was enhanced by IL-1beta in HTSMCs transfected with MMP-9-Luc, which was inhibited by helenalin, U0126, SB202190, and SP600125
Bahlis et al., Blood 2007 (Multiple Myeloma) : We have found that direct activation of myeloma cell CD28 by anti-CD28 mAb alone induces activation of PI3K and NFkappaB , suppresses MM cell proliferation, and protects against serum starvation and dexamethasone ( dex ) -induced cell death
Toshchakov et al., J Immunol 2007 (MAP Kinase Signaling System) : TLR2- and TLR4-BB peptides ( BBPs ) inhibited NF-kappaB translocation and early IL-1beta mRNA expression induced by LPS, and the lipopeptides S- [ 2,3-bis ( palmitoyloxy ) - ( 2-RS ) -propyl ] -N-palmitoyl- ( R ) -Cys-Ser-Lys ( 4 ) -OH ( P3C ) and S- [ 2,3-bis ( palmitoyloxy ) - ( 2-RS ) -propyl ] -Cys-Ser-Lys ( 4 ) -OH ( P2C )
García-García et al., J Immunol 2007 (MAP Kinase Signaling System) : WT FcgammaRIIA and mutants that associated with lipid rafts efficiently activated NF-kappaB , in an ERK dependent manner ... WT FcgammaRIIA and mutants that associated with lipid rafts efficiently activated NF-kappaB , in an ERK dependent manner
Sui et al., J Immunol 2007 (AIDS Dementia Complex...) : rCD40L synergized with HIV-1 Tat to increase TNF-alpha release from primary human monocytes and microglia, in an NF-kappaB dependent manner
Hou et al., Arch Biochem Biophys 2007 : The results demonstrated that PDGG suppressed COX-2 expression via blocking MAPK mediated activation of nuclear factor-kappaB (NF-kappaB) , activator protein-1 (AP-1) and C/EBPdelta
Yang et al., J Mol Cell Cardiol 2007 (Disease Models, Animal...) : Thus, IL-6 mediated up-regulation of cardiac NF-kappaB , ICAM-1, CINC-1, -3, and MPO activity likely contributes to altered cardiac function following trauma-hemorrhage
Hong et al., J Biol Chem 2007 : Interestingly, peptides spanning CCR2 and/or LZ disrupt IKKgamma-Tax and IKKgamma-PP2A interactions and potently inhibit NF-kappaB activation by Tax and tumor necrosis factor-alpha ... Interestingly, peptides spanning CCR2 and/or LZ disrupt IKKgamma-Tax and IKKgamma-PP2A interactions and potently inhibit NF-kappaB activation by Tax and tumor necrosis factor-alpha ... Interestingly, peptides spanning CCR2 and/or LZ disrupt IKKgamma-Tax and IKKgamma-PP2A interactions and potently inhibit NF-kappaB activation by Tax and tumor necrosis factor-alpha ... Interestingly, peptides spanning CCR2 and/or LZ disrupt IKKgamma-Tax and IKKgamma-PP2A interactions and potently inhibit NF-kappaB activation by Tax and tumor necrosis factor-alpha
Dogra et al., FASEB J 2007 (Body Weight...) : Furthermore, TWEAK increased the activation of NF-kappaB transcription factor in myotubes
Xu et al., Rheumatology (Oxford) 2007 (Arthritis, Rheumatoid) : Artesunate also prevented TNF-alpha induced nuclear NF-kappaB translocation, DNA binding activity and gene transcriptional activity, as well as phosphorylation and degradation of IkappaBalpha, but phosphorylation of p38 mitogen activated protein kinase, extracellular signal regulated kinase and c-Jun N-terminal kinase were unaffected
Djoko et al., J Agric Food Chem 2007 (Inflammation) : As shown by NF-kappaB-driven luciferase assay, LPS induced NF-kappaB activities were also reduced by the stilbenoids
Ryan et al., Biochem Biophys Res Commun 2007 (Anoxia) : Furthermore, depletion of p38 using siRNA significantly reduces IHR induced NF-kappaB activity ... Thus, IHR activates NF-kappaB in an IKK dependent manner signaled at least in part via activation of p38 MAPK
Wang et al., Biochem Biophys Res Commun 2007 (Lung Neoplasms) : The results show that Akt contributes to TNF induced NF-kappaB activation in lung cancer cells through regulating phosphorylation of the p65/RelA subunit of NF-kappaB ... The results show that Akt contributes to TNF induced NF-kappaB activation in lung cancer cells through regulating phosphorylation of the p65/RelA subunit of NF-kappaB ... Although individually blocking IKK or Akt partially suppressed TNF induced NF-kappaB activation, concurrent suppression of these pathways completely inhibited TNF induced NF-kappaB activation and downstream anti-apoptotic gene expression, and synergistically potentiated TNF induced cytotoxicity ... Although individually blocking IKK or Akt partially suppressed TNF induced NF-kappaB activation, concurrent suppression of these pathways completely inhibited TNF induced NF-kappaB activation and downstream anti-apoptotic gene expression, and synergistically potentiated TNF induced cytotoxicity ... Although individually blocking IKK or Akt partially suppressed TNF induced NF-kappaB activation, concurrent suppression of these pathways completely inhibited TNF induced NF-kappaB activation and downstream anti-apoptotic gene expression, and synergistically potentiated TNF induced cytotoxicity
Hsu et al., Food Chem Toxicol 2007 (Carcinoma, Hepatocellular...) : Furthermore, EAC also inhibited the TNF-alpha activated NF-kappaB dependent reporter gene expression of MMP-9 and VEGF , and the invasion of cancer cells ... Furthermore, EAC also inhibited the TNF-alpha activated NF-kappaB dependent reporter gene expression of MMP-9 and VEGF, and the invasion of cancer cells
Jamaluddin et al., Cell Signal 2007 : These results indicate that the ROS mediated TNF-alpha induced IL-8 transcription is regulated by NF-kappaB/RelA phosphorylation at the critical Ser ( 276 ) residue by PKAc, resulting in stable enhanceosome formation on target genes
Schweitzer et al., EMBO J 2007 : CSN controls NF-kappaB by deubiquitinylation of IkappaBalpha ... For the first time, we show here an inducible interaction of the CSN with IkappaBalpha and that the CSN controls IkappaBalpha and NF-kappaB activity ... Strikingly, disruption of the CSN by a small interfering RNA mediated knockdown of single CSN subunits results in a reduced re-accumulation of IkappaBalpha and prolonged nuclear translocation of NF-kappaB in TNFalpha stimulated cells
Sarkar et al., Mol Cell Biochem 2007 : Induction of COX-2 by acrolein was significant at 6 h post-treatment and was dependent upon NFkappaB activation ... The activation of NFkappaB by acrolein was induced as a result of degradation of IkappaBalpha over the time of treatment
Kumar et al., Bioorg Med Chem 2007 : To elucidate the molecular mechanism of inhibition of cell adhesion molecules, we investigated the status of nuclear transcription factor-kappaB ( NF-kappaB ) and were able to establish that compound 8a significantly blocked the TNF-alpha induced activation of NF-kappaB
Newton et al., J Pharmacol Exp Ther 2007 (Pulmonary Disease, Chronic Obstructive) : In A549 cells, interleukin (IL)-1beta and tumor necrosis factor (TNF) alpha increased phosphorylation of IkappaBalpha , and this was followed by loss of IkappaBalpha, induction of NF-kappaB DNA binding, and the induction of NF-kappaB dependent transcription ... In contrast, the corticosteroid, dexamethasone, was without effect on NF-kappaB dependent transcription or the expression of ICAM-1
Tirumurugaan et al., Am J Physiol Lung Cell Mol Physiol 2007 (MAP Kinase Signaling System) : We determined the role of mitogen activated protein kinase ( MAPK ) in the activation of NF-kappaB and AP-1 in the regulation of CD38 expression in HASM cells ... EMSAs confirmed the role of p38 and JNK in mediating NF-kappaB and AP-1 activation ... These results indicate that regulation of CD38 expression through p38 and JNK MAPKs involves NF-kappaB and AP-1 activation, and ERK and p38 MAPKs also regulate expression posttranscriptionally through message stability
Lee et al., J Vet Sci 2007 : Further study of the mechanism showed that BPA significantly activated extracellular signal regulated kinase ( ERK ) but inhibited anti-apoptotic nuclear factor kappa B (NF-kappaB) activation ... In addition, ERK-specific inhibitor, PD 98,059, reversed BPA induced cell death and restored NF-kappaB activity
Cho et al., Inflammopharmacology 2007 (Helicobacter Infections) : NF-kappaB is activated by phosphorylation of its endogenous inhibitor, IkappaBalpha
Yang et al., Chem Res Toxicol 2007 (Necrosis) : It reduces mitochondrial membrane potential by activating calpain and inhibits NF-kappaB activity by increasing the ROS level
Ma et al., Invest Ophthalmol Vis Sci 2007 : RAGE mediated upregulation of VEGF secretion by ARPE-19 cells was largely dependent on NF-kappaB , as indicated by studies with parthenolide ... NF-kappaB plays a central role in RAGE dependent RPE secretion of VEGF ... NF-kappaB plays a central role in RAGE dependent RPE secretion of VEGF
von Knethen et al., J Cell Biol 2007 : In these cells, we observed PPARgamma dependent inhibition of nuclear factor-kappaB (NF-kappaB) activation and TNF-alpha expression in response to PMA
Figarola et al., Diabetes 2007 (Inflammation) : These inhibitory effects may be exerted via inhibition of nuclear factor-kappaB (NF-kappaB) activation, as LR-90 suppressed both S100b-and tumor necrosis factor-alpha induced IkappaB-alpha degradation as well as NF-kappaB promoter transcriptional activity
Hattori et al., Diabetes 2007 : gAd activated NF-kappaB and enhanced tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activity
Aifantis et al., Cell cycle (Georgetown, Tex.) 2007 (Cell Transformation, Neoplastic...) : Our studies suggested that NFkappaB activation by Notch1 can be direct, as Notch1 can bind and activate the promoters of the RELB and NFkappaB2 factors and indirect, as Notch1 can form a complex with the NFkappaB kinase IKK
Griessinger et al., Leukemia 2007 (Leukemia, Myeloid, Acute) : Using Ba/F3 murine pre-B cells transfected with various mutants of FLT3 ( ITD, D835V, D835Y ) and the MV4-11 human AML line, we show that normal or oncogenic stimulation of FLT3 led to activation of NF-kappaB
Leshinsky-Silver et al., Int J Colorectal Dis 2007 (Crohn Disease) : NFkappaB plays a critical role in the immune response and is down-regulated by NFkappaB inhibitor alpha (NFKBIA)
Song et al., Exp Mol Med 2007 : In this study, we examined the effect of HIV-1 Tat on the expression of adhesion molecules, generation of reactive oxygen species ( ROS ) and NF-kappaB activation in CRT-MG human astroglioma cells ... In addition, Tat induced the activation of NF-kappaB in astrocytes ... Inhibition of Tat induced ROS generation by N-acetyl cysteine, vitamin C and diphenyl iodonium suppressed Tat induced NF-kappaB activation, ICAM-1 and VCAM-1 expression, and monocyte adhesion in CRT-MG
Lee et al., Exp Mol Med 2007 (Leukemia) : We conclude that TNF-alpha induces upregulation of PTEN expression through NF-kappaB activation in human leukemic cells ... We conclude that TNF-alpha induces upregulation of PTEN expression through NF-kappaB activation in human leukemic cells
Lucas et al., Oncogene 2007 : A dual role for the API2 moiety in API2-MALT1 dependent NF-kappaB activation : heterotypic oligomerization and TRAF2 recruitment ... A dual role for the API2 moiety in API2-MALT1 dependent NF-kappaB activation : heterotypic oligomerization and TRAF2 recruitment ... Thus, API2 moiety mediated heterotypic oligomerization and TRAF2 binding both contribute to maximal API2-MALT1 dependent NF-kappaB stimulation ... Thus, API2 moiety mediated heterotypic oligomerization and TRAF2 binding both contribute to maximal API2-MALT1 dependent NF-kappaB stimulation
Tipton et al., J Periodontol 2007 (Periodontitis) : PGE ( 2 ) and the transcription factor nuclear factor-kappa B (NF-kappaB) regulate IL-1beta stimulated IL-6 production
Shimizu et al., J Hepatol 2007 (Hemorrhage...) : PPT reduced hepatic NF-kappaB and AP-1 activity and iNOS expression
Oh et al., Biochem Biophys Res Commun 2007 : Furthermore, an anti-IFN-gamma antibody strongly inhibits NO production and an inhibitor of NF-kappaB reduces the level of induction of inducible NO synthase (iNOS) in MSCs. Taken together, our results suggest that NO plays a significant role in the modification of Th1 and Th2 differentiation by MSCs, and that both IFN-gamma and NF-kappaB are critical for NO production by MSCs
Koziczak-Holbro et al., J Biol Chem 2007 (Inflammation) : IRAK-4 KD cells are severely impaired in NFkappaB , JNK, and p38 activation in response to IL-1beta or TLR7 ligand
Mburu et al., Immunol Res 2006 (Carcinoma, Squamous Cell...) : Based on our recent findings, the induction of CCR7 expression and the sustenance of the autocrine signaling pathway have been shown to be regulated by NF-kappaB , similar to several types of immune cells
Hong et al., J Nat Prod 2007 : Kaurane diterpenoids from Isodon excisus inhibit LPS induced NF-kappaB activation and NO production in macrophage RAW264.7 cells ... All of the isolates were evaluated for their inhibitory effects on LPS induced NF-kappaB activation and nitric oxide production in RAW264.7 cells
Wang et al., BMC molecular biology 2007 : NF-kappaB mediates the transcription of mouse calsarcin-1 gene, but not calsarcin-2, in C2C12 cells ... NF-kappaB mediates the transcription of mouse calsarcin-1 gene, but not calsarcin-2 , in C2C12 cells ... Our present data suggest that NF-kappaB is required for the transcription of mouse CS-1 but not CS-2, and that the regulation of the calsarcins is mediated also by the NFAT and MEF2 transcription factors
Hellweg et al., Ann N Y Acad Sci 2006 : For characterization of the cell based assay, activation of the pathway by several agents, for example, tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta), lipopolysaccharide (LPS), camptothecin and phorbol ester ( PMA ), and the influence of the culture conditions on NF-kappaB activation by TNF-alpha were examined ... NF-kappaB was activated by TNF-alpha , IL-1beta, PMA, and camptothecin in a dose dependent manner, but not by LPS ... NF-kappaB was activated by TNF-alpha, IL-1beta , PMA, and camptothecin in a dose dependent manner, but not by LPS ... NF-kappaB was activated by TNF-alpha, IL-1beta , PMA, and camptothecin in a dose dependent manner, but not by LPS ... NF-kappaB was activated by TNF-alpha, IL-1beta, PMA, and camptothecin in a dose dependent manner, but not by LPS
Zhuo et al., Ann N Y Acad Sci 2006 : Our results therefore suggest that AT(1) receptor mediated endocytosis of extracellular Ang II may be required for Ang II-induced NF-kappaB activation and subsequent cell proliferation in renal proximal tubule cells
Mustonen et al., Am J Physiol Gastrointest Liver Physiol 2007 : In conclusion, ethanol induces NF-kappaB activation via a calcium dependent pathway and induces COX-2 expression
Lee et al., Glia 2007 (Demyelinating Diseases...) : The activation of NF-kappaB , p38, JNK, and dsRNA dependent protein kinase is required for dsRNA mediated iNOS gene expression
Yu et al., Br J Nutr 2007 : In the present study, we investigated the effects of EA on the formation of intracellular reactive oxygen species, the translocation of NFkappaB and expression of vascular cell adhesion molecule-1 ( VCAM-1 ), intercellular adhesion molecule-1 and endothelial leucocyte adhesion molecule ( E-selectin ) induced by IL-1beta in human umbilical vein endothelial cells ( HUVEC )
Carmody et al., Cell Mol Immunol 2007 (Infection) : Many of the key molecular events required for TLR induced NF-kappaB activation have been elucidated
Qin et al., Cell Mol Immunol 2007 (Acute Disease...) : Electrophoretic mobility shift assay ( EMSA ) revealed that TNF-alpha induced NF-kappaB transactivation in AML14 cells in a time- and dose dependent fashion, and subsequent supershift assays indicated that the translocated NF-kappaB was the heterodimer p65 ( RelA)/p50
Tassiulas et al., Hum Immunol 2007 (MAP Kinase Signaling System) : Apoptotic cells had minimal effects on LPS induced activation of NF-kappaB and MAPKs, but induced expression of SOCS proteins and substantially suppressed induction of CXCL10 expression by IFN-alpha
Bal et al., Hum Mutat 2007 (Ectodermal Dysplasia 1, Anhidrotic) : Our results confirm that NF-kB activation is impaired in EDA and support the role of EDARADD DD as a downstream effector of EDAR signaling
Legrand-Poels et al., J Cell Sci 2007 : Modulation of Nod2 dependent NF-kappaB signaling by the actin cytoskeleton ... We showed that actin disruption by CytD significantly and specifically increased Nod2 mediated NF-kappaB signaling ... Membrane ruffle disruption by a Rac1 dominant negative form primed Nod2 dependent NF-kappaB signaling ... The recruitment of Nod2 in Rac induced dynamic cytoskeletal structures could be a strategy to both repress the Nod2 dependent NF-kappaB signaling in unstimulated cells and rapidly mobilize Nod2 during bacterial infection
Li et al., J Huazhong Univ Sci Technolog Med Sci 2006 : NF-kappaB activity induced by TNF-alpha was significantly increased in AM from patients with COPD, and pyrrolidine dithiocarbamate ( PDTC ) and N-acetyl-L-cysteine (NAC) significantly inhibited the activation of NF-kappaB induced by TNF-alpha ( P < 0.05 ) ... NF-kappaB activity induced by TNF-alpha was significantly increased in AM from patients with COPD, and pyrrolidine dithiocarbamate ( PDTC ) and N-acetyl-L-cysteine (NAC) significantly inhibited the activation of NF-kappaB induced by TNF-alpha ( P < 0.05 )
Takahashi et al., Dig Dis Sci 2007 (Pancreatic Neoplasms) : The NF-kappaB activity of both cell lines was increased by the addition of TNF-alpha, while TNF-alpha induced NF-kappaB activity was suppressed by prestimulation with GM in a dose dependent manner ... These results indicate that GM inhibits TNF-alpha induced NF-kappaB activation and enhances apoptosis in human pancreatic cancer cell lines
Jung et al., Neurol Res 2007 : In addition, EMSA experiment revealed that Hizikia fusiforme blocked the LPS induced activation of NFkappab
Savković et al., Biochem Biophys Res Commun 2007 (Disease Models, Animal...) : Presence of clusterin reduced NF-kappaB activation and expression of the NF-kappaB target genes TNF-alpha and MOB-1 under cell stress
Zhang et al., Proc Natl Acad Sci U S A 2007 (Inflammation...) : This LA-dependent increase in Akt phosphorylation was accompanied by inhibition of LPS induced NF-kappaB DNA binding activity and up-regulation of TNFalpha and monocyte chemoattractant protein 1 ... Lipoic acid dependent Akt phosphorylation and inhibition of NF-kappaB activity were abolished by the PI3K inhibitors LY294002 and wortmannin
Lu et al., Proc Natl Acad Sci U S A 2007 : Similarly high concentrations of each of these cytokines cross-activate the other pathway : TGFbeta activates NFkappaB , and IL-1beta activates Smads ... Knockdown experiments revealed that both TGFbeta receptor subunits are required for IL-1beta to activate Smads, and the IL-1 receptor is required for TGFbeta to activate NFkappaB
Miggin et al., Proc Natl Acad Sci U S A 2007 : NF-kappaB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1 ... However, we found that Mal was cleaved by caspase-1 and that inhibition of caspase-1 activity blocked TLR2- and TLR4 mediated NF-kappaB and p38 MAP kinase activation but not IL-1 or TLR7 signaling, which are Mal independent
Park et al., J Mol Biol 2007 : Here, we suggest that Daxx mediated repression of NF-kappaB transactivation correlates with the inhibition of p65 acetylation by Daxx ... Based on the finding that the Daxx binding N-terminal side of p65 includes the major sites of acetylation mediated by p300/CBP, we further propose that the physical interaction between Daxx and p65 provides a functional framework for the inhibition of p65 acetylation by p300/CBP and subsequent repression of NF-kappaB transcriptional activity
Klatt et al., Biologicals 2007 : Furthermore, we evaluated PKR, IL-6, and TNF-alpha induction , as well as IkappaB degradation and NFkappaB activation as control parameters of non-specific siRNA effects
Kamat et al., Mol Cancer Ther 2007 (Urinary Bladder Neoplasms) : Both gemcitabine and TNF activated NF-kappaB in bladder cancer cells and curcumin suppressed this activation
Weisz et al., Cancer Res 2007 (Breast Neoplasms...) : We now report that cancer associated mutant p53 can augment the induction of nuclear factor kappaB (NFkappaB) transcriptional activity in response to the cytokine tumor necrosis factor alpha (TNFalpha) ... We now report that cancer associated mutant p53 can augment the induction of nuclear factor kappaB (NFkappaB) transcriptional activity in response to the cytokine tumor necrosis factor alpha (TNFalpha) ... Analysis of human head and neck tumors and lung tumors reveals a close correlation between the presence of abundant mutant p53 proteins and the constitutive activation of NFkappaB
Matthews et al., Cancer Res 2007 (Skin Neoplasms) : Dominant negative c-Jun ( TAM67 ) blocks AP-1 activation by dimerizing with Jun or Fos family proteins and blocks NFkappaB activation by interacting with NFkappaB p65
Gupta et al., Cancer Res 2007 (Breast Neoplasms) : Suppressing Smad1 expression using small interfering RNA also mitigated MIS induced Gro-beta mRNA, suggesting that regulation of Gro-beta expression by MIS was dependent on activation of NF-kappaB as well as Smad1 ... These results show a novel interaction between MIS stimulated Smad1 and NF-kappaB signaling in which enhancement of NF-kappaB DNA binding and gene expression by phospho-Smad1 is dependent on the sequence of the kappaB consensus site within the promoter ... These results show a novel interaction between MIS stimulated Smad1 and NF-kappaB signaling in which enhancement of NF-kappaB DNA binding and gene expression by phospho-Smad1 is dependent on the sequence of the kappaB consensus site within the promoter
Wu et al., EMBO Rep 2007 : Tax activates NF-kappaB by stimulating the activity of IkappaB kinase (IKK), but the underlying mechanism remains elusive ... Furthermore, unlike cellular stimuli, the Tax-specific NF-kappaB signalling does not require the ubiquitin binding function of IKKgamma
Hijioka et al., Biochem Biophys Res Commun 2007 : While the crosslinking activated NF-kappaB and induced its target gene, IkappaB-alpha, co-crosslinking of Fcgamma receptor IIB to the surface immunoglobulin complex inhibited NF-kappaB activation and the induction of IkappaB-zeta and IkappaB-alpha, suggesting critical roles for NF-kappaB in the induction ... While the crosslinking activated NF-kappaB and induced its target gene, IkappaB-alpha, co-crosslinking of Fcgamma receptor IIB to the surface immunoglobulin complex inhibited NF-kappaB activation and the induction of IkappaB-zeta and IkappaB-alpha , suggesting critical roles for NF-kappaB in the induction
Liang et al., Brain Res 2007 (Nerve Degeneration...) : Blockade of NF-kappaB nuclear translocation with recombinant cell-permeable peptide NF-kappaB SN50 inhibited NF-kappaB nuclear translocation and p53 induction
Wang et al., Nat Cell Biol 2007 (Breast Neoplasms...) : ERalpha signalling reduced levels of functional NF-kappaB and Fra-2 AP-1 and inhibited de novo RelB synthesis, leading to an inverse correlation between RELB and ERalpha gene expression in human breast cancer tissues and cell lines ... ERalpha signalling reduced levels of functional NF-kappaB and Fra-2 AP-1 and inhibited de novo RelB synthesis, leading to an inverse correlation between RELB and ERalpha gene expression in human breast cancer tissues and cell lines
Bieler et al., Oncogene 2007 : Here we report that integrin ligation acts permissive for TNF induced protein kinase B ( PKB/Akt ) but not nuclear factor (NF)-kappaB activation
Lawrence et al., Biochem Soc Trans 2007 (Inflammation) : The NF-kappaB ( nuclear factor kappaB ) pathway is an important regulator of inflammation and immunity ; NF-kappaB activation is controlled by IKK [ IkappaB ( inhibitor of NF-kappaB ) kinase ] complex, which regulates NF-kappaB activation in response to pro-inflammatory stimuli
Chiu et al., Blood 2007 : Gel shifting and chromatin immunoprecipitation assays showed that SMC coculture increased the nuclear factor-kappaB (NF-kappaB)-promoter binding activity in ECs ; inhibition of NF-kappaB activation by p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture induced E-selectin promoter activity
Fionda et al., J Immunol 2007 : 15-deoxy-Delta12,14-prostaglandin J2 negatively regulates rankl gene expression in activated T lymphocytes : role of NF-kappaB and early growth response transcription factors
Dubrac et al., J Immunol 2007 : Moreover, PPARalpha activation inhibits NF-kappaB but not stress activated protein kinase/JNK, p38MAPK, and ERK1/2
Sullivan et al., J Immunol 2007 : TICAM1 activates NF-kappaB through two different pathways involving its interactions with TNFR associated factor 6 and receptor interacting protein 1 ... In this study, we describe the role of zebrafish ( Danio rerio ) TICAM1 in activating NF-kappaB and zebrafish type I IFN ... Furthermore, zebrafish TICAM1 does not interact with zebrafish TNFR associated factor 6, thus NF-kappaB activation is dependent upon its interaction with receptor interacting protein 1
Lee et al., Carcinogenesis 2007 (MAP Kinase Signaling System) : Humulone inhibits phorbol ester induced COX-2 expression in mouse skin by blocking activation of NF-kappaB and AP-1 : IkappaB kinase and c-Jun-N-terminal kinase as respective potential upstream targets ... The roles of extracellular signal regulated protein kinase-1/2 and p38 MAPK in TPA induced activation of NF-kappaB in mouse skin had been defined in our previous studies
Yoon et al., J Agric Food Chem 2007 (Breast Neoplasms) : The present study examines the effects of selected phytochemicals and apple extracts on TNF-alpha induced NF-kappaB activation in human breast cancer MCF-7 cells ... Apple extracts significantly inhibited the TNF-alpha induced NF-kappaB activation at a dose of 5 mg/mL ( p < 0.05 ) ... Curcumin also significantly blocked the TNF-alpha induced NF-kappaB activation at doses of 10 and 20 microM ( p < 0.05 ) ... These results suggest that apple extracts and curcumin have the capabilities of inhibiting TNF-alpha induced NF-kappaB activation of MCF-7 cells by inhibiting the proteasomal activities instead of IkappaB kinase (IKK) activation
Wagner-Ballon et al., Blood 2007 (Disease Models, Animal...) : We first demonstrated that bortezomib was able to inhibit TPO induced NF-kappaB activation in vitro in murine megakaryocytes
Hong et al., Life Sci 2007 : These results suggest that EGCG may exert an anti-inflammatory effect in endothelial cells by controlling MCP-1 expression, at least in part, mediated through the suppression of p38 MAPK and NF-kappaB activation
Dugo et al., Novartis Found Symp 2007 (Endotoxemia...) : GSK-3beta inhibition reduced the increase in NF-kappaB p65 activity caused by interleukin (IL)1 in human e mbryonic kidney cells in vitro
Lindholm et al., J Virol 1992 : Tax1 induction of NF-kappa B occurred in the presence of cycloheximide, and Tax1 stimulation did not result in increased levels of NF-kappa B or c-rel RNA
Gallagher et al., Free Radic Biol Med 2007 : Interestingly, inhibition of NF-kappaB led to a marked increase in Prdx6 regulation in the absence or presence of inducers, suggesting a normal role for NF-kappaB in Prdx6 suppression
Puthenedam et al., Cell Biol Int 2007 (Escherichia coli Infections) : NF-kappaB inhibitor slightly blocked the decrease in TER and protected against actin disruption while ERK1/2 inhibitor had no effect in blocking these changes
Reyes-Gordillo et al., Biochim Biophys Acta 2007 (Carbon Tetrachloride Poisoning...) : Administration of CCl4 induced the translocation of NF-kappaB to the nucleus ; CCl4 induced NF-kappaB DNA binding activity was blocked by curcumin treatment ... Administration of CCl4 induced the translocation of NF-kappaB to the nucleus ; CCl4 induced NF-kappaB DNA binding activity was blocked by curcumin treatment
Nishinakamura et al., Int Immunol 2007 : An RNA binding protein alphaCP-1 is involved in the STAT3 mediated suppression of NF-kappaB transcriptional activity ... An RNA binding protein alphaCP-1 is involved in the STAT3 mediated suppression of NF-kappaB transcriptional activity ... In this study, we investigated the effect of constitutively activated STAT3 ( STAT3C ) on LPS induced nuclear factor-kappaB (NF-kappaB) activation ... In this study, we investigated the effect of constitutively activated STAT3 ( STAT3C ) on LPS induced nuclear factor-kappaB (NF-kappaB) activation ... The forced expression of STAT3C in HEK293/TLR4 cells, but neither wild-type STAT3 nor dominant negative form of STAT3, suppressed LPS-TLR4 mediated NF-kappaB reporter activation ... The forced expression of STAT3C in HEK293/TLR4 cells, but neither wild-type STAT3 nor dominant negative form of STAT3, suppressed LPS-TLR4 mediated NF-kappaB reporter activation ... The forced expression of STAT3C in HEK293/TLR4 cells, but neither wild-type STAT3 nor dominant negative form of STAT3, suppressed LPS-TLR4 mediated NF-kappaB reporter activation ... Thus, STAT3C could suppress the transcriptional and/or translational activity of NF-kappaB ... Thus, STAT3C could suppress the transcriptional and/or translational activity of NF-kappaB ... These data suggest that alphaCP-1 is involved in the STAT3 mediated suppression of NF-kappaB activity ... These data suggest that alphaCP-1 is involved in the STAT3 mediated suppression of NF-kappaB activity
Ke et al., J Dent Res 2007 (Temporomandibular Joint Disorders) : Role of NF-kappaB in TNF-alpha induced COX-2 expression in synovial fibroblasts from human TMJ ... Here we investigated the role of Nuclear Factor kappaB (NF-kappaB) in the regulation of COX-2 expression in synovial fibroblasts from human TMJ induced by tumor necrosis factor-alpha (TNF-alpha) ... Electrophoretic mobility shift assay ( EMSA ) revealed that transient NF-kappaB activation in the COX-2 promoter was triggered by TNF-alpha ... These findings indicate that activation of NF-kappaB is responsible for TNF-alpha induced COX-2 expression in synovial fibroblasts from the TMJ
Rajesh et al., Am J Physiol Heart Circ Physiol 2007 : HG markedly increased mitochondrial superoxide generation ( measured by flow cytometry using MitoSOX ), NF-kappaB activation , nitrotyrosine formation, upregulation of iNOS and adhesion molecules ICAM-1 and VCAM-1 , transendothelial migration of monocytes, and monocyte-endothelial adhesion in HCAECs ... HG markedly increased mitochondrial superoxide generation ( measured by flow cytometry using MitoSOX ), NF-kappaB activation , nitrotyrosine formation, upregulation of iNOS and adhesion molecules ICAM-1 and VCAM-1, transendothelial migration of monocytes, and monocyte-endothelial adhesion in HCAECs ... HG markedly increased mitochondrial superoxide generation ( measured by flow cytometry using MitoSOX ), NF-kappaB activation , nitrotyrosine formation, upregulation of iNOS and adhesion molecules ICAM-1 and VCAM-1, transendothelial migration of monocytes, and monocyte-endothelial adhesion in HCAECs ... In this study we have investigated the effects of CBD on high glucose ( HG ) -induced, mitochondrial superoxide generation, NF-kappaB activation, nitrotyrosine formation, inducible nitric oxide synthase (iNOS) and adhesion molecules ICAM-1 and VCAM-1 expression, monocyte-endothelial adhesion, transendothelial migration of monocytes, and disruption of endothelial barrier function in human coronary artery endothelial cells ( HCAECs )
Cho et al., Ann N Y Acad Sci 2006 (Helicobacter Infections) : In conclusion, H. pylori activates Ras, NF-kappaB , and AP-1 and thus induces the expression of integrin alpha5 and integrin beta1 in gastric epithelial cells ... In conclusion, H. pylori activates Ras, NF-kappaB , and AP-1 and thus induces the expression of integrin alpha5 and integrin beta1 in gastric epithelial cells
Ju et al., Ann N Y Acad Sci 2006 : Role of mitogen activated protein kinases, NF-kappaB , and AP-1 on cerulein induced IL-8 expression in pancreatic acinar cells
Duan et al., Lab Invest 2007 (Inflammation...) : Additionally, cells expressing constitutively active beta-catenin showed IkappaBalpha stabilization and inhibition of NF-kappaB activity not only after WT Salmonella infection but also after commensal bacteria ( Escherichia coli F18 ) and TNF-alpha treatment
Liang et al., J Neurosci Res 2007 : To evaluate whether nuclear factor-kappaB (NF-kappaB) activation promotes cell cycle entry and neuronal apoptosis, we studied the relation among NF-kappaB mediated cyclin induction, bromodeoxyuridine ( BrdU ) incorporation, and apoptosis initiation in rat striatal neurons following excitotoxic insult ... These findings suggest that NF-kappaB activation stimulates cyclin D1 expression and triggers DNA replication in striatal neurons
Erkel et al., Int Immunopharmacol 2007 (Autoimmune Diseases...) : Panepoxydone strongly inhibited hTNF-alpha, IL-8 and NF-kappaB promoter activity in LPS/TPA stimulated MonoMac6 cells with IC ( 50 ) values of 0.5-1 microg/ml by blocking the phosphorylation of IkappaB and subsequent binding of the activated NF-kappaB transcription factor to the DNA
Sung et al., Mol Pharmacol 2007 : We found that among nine different flavones tested, fisetin was potent in suppressing tumor necrosis factor (TNF) induced NF-kappaB activation ... NF-kappaB dependent reporter gene expression was also suppressed by fisetin, as was NF-kappaB reporter activity induced by TNFR1 , TRADD, TRAF2, NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by fisetin, as was NF-kappaB reporter activity induced by TNFR1, TRADD , TRAF2, NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by fisetin, as was NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2 , NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by fisetin, as was NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2 , NIK, and IKK but not that induced by p65 transfection ... NF-kappaB dependent reporter gene expression was also suppressed by fisetin, as was NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2, NIK, and IKK but not that induced by p65 transfection
Yang et al., Mol Cell Biochem 2007 : Identification of the distinct promoters for the two transcripts of apoptosis related protein 3 and their transcriptional regulation by NFAT and NFkappaB ... In contrast, exogenous expression of a constitutively active mutant of the transcription factor NFkB inhibited APR3 transcription
Miller et al., Invest Ophthalmol Vis Sci 2007 : Lactate treatment resulted in dose- and time dependent effects on human TM cell viability, translocation of NF-kappaB , and activation of MT1-MMP
Adrogue et al., Am J Nephrol 2007 (Nephrosis, Lipoid) : Involvement of Th2 T cells/NFkappaB in minimal change disease (MCD) has been postulated
de Kozak et al., Am J Pathol 2007 (Inflammation...) : At 24 hours after lipopolysaccharide stimulation, nuclear NF-kappaB/p65 decreased and nuclear NF-kappaB/p50 increased , associated with nuclear BCL-3 and a low level of TNF-alpha, indicating onset of spontaneous resolution
Jin et al., J Biol Chem 2007 (Hyperplasia) : These findings demonstrate a critical role for CYLD in regulating the basal activity of NF-kappaB and maintaining the naive phenotype and proper activation of B cells
Kang et al., Arthritis Rheum 2007 (Arthritis, Rheumatoid...) : LIGHT mediated up-regulation of MMPs and intercellular adhesion molecule 1 was blocked by inhibitors of NF-kappaB and JNK, whereas up-regulation of vascular cell adhesion molecule 1 was blocked by inhibitors of phosphatidylinositol 3-kinase, as well as NF-kappaB ... LIGHT mediated up-regulation of MMPs and intercellular adhesion molecule 1 was blocked by inhibitors of NF-kappaB and JNK, whereas up-regulation of vascular cell adhesion molecule 1 was blocked by inhibitors of phosphatidylinositol 3-kinase, as well as NF-kappaB
Yang et al., Free Radic Biol Med 2007 (Melanoma...) : In contrast, NAC inhibits NF-kappaB activation by reducing inhibitor of kappaB kinase (IKK) activity ... In contrast, NAC inhibits NF-kappaB activation by reducing inhibitor of kappaB kinase (IKK) activity
Wosik et al., J Neurol Sci 2007 : TNFR1 ligation induces NFkappaB activation and the upregulation of chemokines MCP-1 and IL-8, as well as adhesion molecules ICAM-1 and VCAM-1, while Fas and DR5 triggering activate the extracellular signal regulated kinases-1 and -2 ( Erk 1/2, p42/44 MAPK ) inducing the release of matrix metalloproteinase 9 (MMP9) by BBB derived ECs ... TNFR1 ligation induces NFkappaB activation and the upregulation of chemokines MCP-1 and IL-8, as well as adhesion molecules ICAM-1 and VCAM-1, while Fas and DR5 triggering activate the extracellular signal regulated kinases-1 and -2 ( Erk 1/2, p42/44 MAPK ) inducing the release of matrix metalloproteinase 9 (MMP9) by BBB derived ECs ... TNFR1 ligation induces NFkappaB activation and the upregulation of chemokines MCP-1 and IL-8, as well as adhesion molecules ICAM-1 and VCAM-1, while Fas and DR5 triggering activate the extracellular signal regulated kinases-1 and -2 ( Erk 1/2, p42/44 MAPK ) inducing the release of matrix metalloproteinase 9 (MMP9) by BBB derived ECs
Chan et al., J Physiol 2007 (Brain Death...) : Using a clinically relevant animal model that employed the organophosphate pesticide mevinphos ( Mev ) as the experimental insult, we evaluated the hypothesis that transcriptional up-regulation of nitric oxide synthase I or II ( NOS I or II ) gene expression by nuclear factor-kappaB (NF-kappaB) on activation of muscarinic receptors in the RVLM underlies brain stem death
Kye et al., Mol Cell Biochem 2007 : Finally, we showed that activation of GRs but not of MRs repressed the NF-kappaB mediated induction of SK2-S transcription
Rho et al., Toxicol Appl Pharmacol 2007 : Palmitate inhibited an increase of TNF-alpha mediated nuclear factor kappa B (NF-kappaB) activity, the survival pathway activated by the cytokine, by hindering the translocation of the NF-kappaB subunit of p65 from the cytosol into the nucleus
Poulaki et al., FASEB J 2007 (Leukostasis...) : 17-AAG treatment significantly suppressed the LPS induced increase in retinal leukocyte adhesion ; vascular leakage ; NF-kappaB , HIF-1alpha, p38, and PI-3K activity ; and VEGF, TNF-alpha, and IL-1beta levels
Lu et al., Br J Pharmacol 2007 : The effect of YC-1 on LPS induced activation of nuclear factor kappa B (NF-kappaB) was studied by NF-kappaB reporter assay and immunofluorocytochemistry ... Furthermore, YC-1 inhibited LPS induced NF-kappaB activation in microglia ... YC-1 was able to inhibit LPS induced iNOS and COX-2 expression and NF-kappaB activation, indicating that YC-1 may be developed as an anti-inflammatory neuroprotective agent
Matsumoto et al., FEBS J 2007 (MAP Kinase Signaling System) : For example, FasL induces nuclear factor kappaB (NF-kappaB) activity and interleukin 8 (IL-8) production by engagement of Fas in human cells ... Selective inhibition of AP-1 did not affect NF-kappaB activation and vice versa, indicating that their activations were not sequential events
Pham et al., Mol Cell Biol 2007 (Neoplasms) : Upregulation of Twist-1 by NF-kappaB blocks cytotoxicity induced by chemotherapeutic drugs ... The mechanism by which NF-kappaB inhibits PCD triggered by chemotherapeutic drugs, however, remains poorly understood
Kundu et al., Ann N Y Acad Sci 2007 : Taken together, EGCG inhibited TPA induced DNA binding of NF-kappaB and CREB by blocking activation of p38 MAPK , which may provide a molecular basis of COX-2 inhibition by EGCG in mouse skin in vivo
Ozaki et al., Clin Cancer Res 2007 (Carcinoma, Hepatocellular...) : Vitamin K2 also suppressed the NF-kappaB binding site dependent cyclin D1 promoter activity and suppressed the basal, 12-O-tetradecanoylphorbol-13-acetate ( TPA ) -, TNF-alpha-, and interleukin (IL)-1 induced activation of NF-kappaB binding and transactivation
Manna et al., Clin Cancer Res 2007 (Inflammation...) : We showed by DNA binding assay that NF-kappaB activation induced by tumor necrosis factor (TNF), phorbol 12-myristate 13-acetate, lipopolysaccharide, ceramide, interleukin-1 , and H ( 2 ) O ( 2 ) was suppressed by morin ; the suppression was not cell type specific ... We showed by DNA binding assay that NF-kappaB activation induced by tumor necrosis factor (TNF) , phorbol 12-myristate 13-acetate, lipopolysaccharide, ceramide, interleukin-1, and H ( 2 ) O ( 2 ) was suppressed by morin ; the suppression was not cell type specific
Mills et al., Proc Natl Acad Sci U S A 2007 : NF-kappaB inducing kinase (NIK) mediated IKKalpha phosphorylation activates the alternative NF-kappaB pathway, which is characterized by nuclear translocation of p52 : RelB heterodimers
Liang et al., J Immunol 2007 (Inflammation) : LT-IIb-B ( 5 ), but not LT-IIb, induced TLR2 mediated NF-kappaB activation and TNF-alpha production
Dijsselbloem et al., J Immunol 2007 : We previously demonstrated that genistein suppresses TNF-alpha induced NF-kappaB dependent IL-6 gene expression in cancer cells by interfering with the mitogen- and stress activated protein kinase 1 activation pathway ... More particularly, we observe that genistein inhibits IL-6 gene expression by modulating the transcription factor NF-kappaB
Nishida et al., Dig Dis Sci 2007 : Geranylgeranylacetone induces cyclooxygenase-2 expression in cultured rat gastric epithelial cells through NF-kappaB
Roulston et al., J Exp Med 1992 (Acquired Immunodeficiency Syndrome) : NF-kappa B binding activity was inducible by tumor necrosis factor and phorbol myristate acetate in PLB-985
Su et al., J Exp Ther Oncol 2007 (Breast Neoplasms) : Reporter assays in MCF-7 cells indicated that Dip1 strongly inhibited the transcriptional activities of the cyclin D1, c-fos, NF-kappaB , SRE and p21cP1 promoters
Horie et al., Microbes Infect 2007 (HIV Infections) : Previous studies indicated that NF-kappaB activation by tumor necrosis factor (TNF) receptor family, which activates TNF receptor associated factor (TRAF) , induces HIV-1 expression
Corcoran et al., Mol Cancer Res 2007 : In summary, NF-kappaB is involved in the repression of Tac1 at higher levels of SDF-1alpha in MCF12A
Jin et al., Biol Pharm Bull 2007 : Six diarylheptanoids ( 1-6 ) from the stem bark of Alnus hirsuta were investigated for their inhibitory activity against LPS induced NF-kB activation and NO and TNF-alpha production
Merentie et al., Amino Acids 2007 (Acute Disease...) : Glutathione level and catalase activity changed transiently, and the effect of Me(2)Spm was clear at 24 h. Serum inflammatory cytokine levels increased already at 4 h whereas NF-kappaB was distinctly activated only at 24 h. Me(2)Spm prevented the increase in TNF-alpha and IL-6 while it had no effect on NF-kappaB activation ... Glutathione level and catalase activity changed transiently, and the effect of Me(2)Spm was clear at 24 h. Serum inflammatory cytokine levels increased already at 4 h whereas NF-kappaB was distinctly activated only at 24 h. Me(2)Spm prevented the increase in TNF-alpha and IL-6 while it had no effect on NF-kappaB activation
Uwagawa et al., Cancer 2007 (Adenocarcinoma...) : In an attempt to identify an effective therapeutic agent for pancreatic cancer, the authors studied the role of FUT-175 , a synthetic serine protease inhibitor, in the inhibition of NF-kappaB activation and the induction of apoptotic responses ... To examine the effect of FUT-175 on the inhibition of NF-kappaB and the induction of apoptosis in pancreatic cancer cell lines, Western and Northern blot analyses, electromobility shift ( EMSA ), luciferase reporter gene, DNA fragmentation, immunoprecipitation, in vitro kinase, small interfering RNA ( siRNA ), and chromatin immunoprecipitation ( ChIP ) assays were performed ... In a time dependent and dose dependent manner, FUT-175 inhibited IkappaBalpha phosphorylation and NF-kappaB activation, thereby inhibiting the antiapoptotic activity of NF-kappaB
Moss et al., Gut 2007 (Colitis...) : UcnII stimulation also led to activation of nuclear factor-kappaB (NF-kappaB) and mitogen acivated protein ( MAP ) kinase in these cells, as evidenced by degradation of IkappaBalpha and phosphorylation of the p65 subunit of NF-kappaB and extracellularly regulated kinase (ERK) 1/2
Li et al., Arterioscler Thromb Vasc Biol 2007 (Atherosclerosis...) : JAK1/TYK2 leads to STAT3 activation, Akt dependent NF-kappaB activation, and phosphorylation of extracellular signal regulated kinase 1/2 and mitogen activated kinase p38 ... JAK1/TYK2 leads to STAT3 activation, Akt dependent NF-kappaB activation , and phosphorylation of extracellular signal regulated kinase 1/2 and mitogen activated kinase p38 ... JAK1/TYK2 leads to STAT3 activation, Akt dependent NF-kappaB activation, and phosphorylation of extracellular signal regulated kinase 1/2 and mitogen activated kinase p38 ... JAK1/TYK2 leads to STAT3 activation, Akt dependent NF-kappaB activation, and phosphorylation of extracellular signal regulated kinase 1/2 and mitogen activated kinase p38 ... JAK1/TYK2 leads to STAT3 activation, Akt dependent NF-kappaB activation , and phosphorylation of extracellular signal regulated kinase 1/2 and mitogen activated kinase p38
Lee et al., Virus Res 2007 (Lung Neoplasms) : IL-6 expression induced by DEN-2 infection was NF-kappaB dependent
Holmes et al., J Biol Chem 2007 : Although NFkappaB nuclear translocation is not altered, FoxP3 enhances NFkappaB-p65 binding to HIV-1 LTR ... Although NFkappaB nuclear translocation is not altered, FoxP3 enhances NFkappaB-p65 binding to HIV-1 LTR
Grohmann et al., Nat Med 2007 (Disease Models, Animal...) : Here we show that reverse signaling through GITRL after engagement by soluble GITR initiates the immunoregulatory pathway of tryptophan catabolism in mouse plasmacytoid dendritic cells, by means of noncanonical NF-kappaB dependent induction of indoleamine 2,3-dioxygenase (IDO)
Gohda et al., Biochem Biophys Res Commun 2007 : HTLV-1 Tax induced NFkappaB activation is independent of Lys-63-linked-type polyubiquitination ... Human T-cell leukemia virus type 1 ( HTLV-1 ) Tax induced activation of nuclear factor-kappaB (NFkappaB) is thought to play a critical role in T-cell transformation and onset of adult T-cell leukemia ... However, the molecular mechanism of the Tax induced NFkappaB activation remains unknown ... However, TAK1 is dispensable for Tax induced NFkappaB activation, and deubiquitination of the K63 polyubiquitin chain failed to block Tax induced NFkappaB activation ... In addition, silencing of other MAP3Ks, including MEKK1, MEKK3, NIK, and TPL-2, did not affect Tax induced NFkappaB activation ... These results strongly suggest that unlike cytokine signaling, Tax induced NFkappaB activation does not involve K63 polyubiquitination mediated MAP3K activation ... These results strongly suggest that unlike cytokine signaling, Tax induced NFkappaB activation does not involve K63 polyubiquitination mediated MAP3K activation
Nie et al., J Allergy Clin Immunol 2007 : ICAM-1 was induced in human and guinea pig parasympathetic nerves by TNF-alpha and IFN-gamma and was inhibited by dexamethasone and by an inhibitor of nuclear factor-kappaB (NF-kappaB)
Fan et al., Mol Immunol 2007 (Inflammation) : beta-Arrestins 1 and 2 have recently been implicated in modulation of NFkappaB signaling and ERK 1/2 activation ... beta-Arrestins 1 and 2 have recently been implicated in modulation of NFkappaB signaling and ERK 1/2 activation ... Using a number of approaches : mouse embryonic fibroblasts (MEF) from wild-type ( WT ), beta-arrestins knockouts ( KO ), beta-arrestins 1 and 2 double KO, and MEFs with reconstituted WT beta-arrestins in the double KO cells, RNA interference ( siRNA ) specific knockdown of beta-arrestins, and overexpression of WT beta-arrestins, it was demonstrated that beta-arrestin 2 positively regulates LPS induced ERK 1/2 activation and both beta-arrestins 1 and 2 negatively regulate LPS induced NFkappaB activation ... beta-Arrestins thus not only negatively regulate LPS induced NFkappaB activation but also positively regulate ERK 1/2 activation and specific pro-inflammatory gene expression ... beta-Arrestins thus not only negatively regulate LPS induced NFkappaB activation but also positively regulate ERK 1/2 activation and specific pro-inflammatory gene expression ... beta-Arrestins thus not only negatively regulate LPS induced NFkappaB activation but also positively regulate ERK 1/2 activation and specific pro-inflammatory gene expression
Caposio et al., Cell Microbiol 2007 : Adenoviral mediated expression of a dominant negative mutant of IKK2 kinase ( dnIKK2 ) in human umbilical vein endothelial cells resulted in a strong reduction of IkappaBalpha degradation and NF-kappaB activation following infection with an HCMV clinical isolate
Fontan et al., FEBS J 2007 : In response to constitutive NF-kappaB stimulation by Tax, we observed NEMO recruitment and oligomerization to a shifted high-molecular-mass complex of 440 kDa which displayed increased IKK activity ... In response to constitutive NF-kappaB stimulation by Tax , we observed NEMO recruitment and oligomerization to a shifted high-molecular-mass complex of 440 kDa which displayed increased IKK activity
Tsuneyoshi et al., Cell Immunol 2006 : The mutant penta acylated LPS from the lpxM-strain did not induce TNF-alpha production in murine peritoneal macrophages, or activation of NF-kappaB in transfected cells expressing murine TLR4/MD-2 ... The mutant penta acylated LPS from the lpxM-strain did not induce TNF-alpha production in murine peritoneal macrophages, or activation of NF-kappaB in transfected cells expressing murine TLR4/MD-2
Dasu et al., Am J Physiol Endocrinol Metab 2007 (MAP Kinase Signaling System) : At the same time, it appears that NADPH oxidase via p47phox activates NF-kappaB , resulting in increased IL-1beta secretion
Muscat et al., Mol Nutr Food Res 2007 : MRPs also induced a two-fold increased NF-kappaB translocation in untransfected human embryonic kidney (HEK) cells as well as in HEK cells stably transfected with the receptor for advanced glycation endproducts ( RAGE ), indicating that the effect was not RAGE mediated ... On the other hand, catalase totally abolished coffee- and MRP induced NF-kappaB translocation
Tanner et al., J Biol Chem 2007 : Earlier studies indicate that CARMA1 , a Caspase Recruitment Domain ( CARD ) and Membrane associated GUanylate Kinase domain ( MAGUK ) -containing scaffold protein, plays an essential role in NF-kappaB activation induced by the costimulation of T cell receptor ( TCR ) and CD28 molecules ... However, the molecular mechanism by which CARMA1 mediates TCR-CD28 costimulation induced NF-kappaB activation is not fully understood ... Disruption of the predicted structure of the Coiled-coil domain of CARMA1 impaired its oligomerization and, importantly, abrogated CARMA1 mediated NF-kappaB activation ... Together, our results demonstrate that the oligomerization of CARMA1 is required for TCR induced NF-kappaB activation
Chávez et al., J Appl Toxicol 2008 (Liver Cirrhosis, Experimental) : Resveratrol prevents fibrosis, NF-kappaB activation and TGF-beta increases induced by chronic CCl4 treatment in rats
Chun et al., J Pharm Pharmacol 2007 : Furthermore, ergolide mediated protein kinase Calpha (PKCalpha) inhibition is involved in reduction of NF-kappaB inhibition, as demonstrated by the observation that dominant negative PKCalpha, but not p44/42 MAPK and p38 MAPK, inhibits TPA stimulated reporter gene expression
May et al., J Biol Chem 2007 : NF-kappaB activation requires the IkappaB kinase (IKK) complex containing two catalytic subunits named IKKalpha and IKKbeta that regulate distinct NF-kappaB pathways ... NF-kappaB activation requires the IkappaB kinase (IKK) complex containing two catalytic subunits named IKKalpha and IKKbeta that regulate distinct NF-kappaB pathways
Oida et al., Mol Cancer Ther 2007 (Lung Neoplasms) : We hypothesized that adenovirus mediated overexpression of melanoma differentiation associated gene-7/interleukin-24 ( Ad-mda7/IL-24 ) induces NF-kappaB expression and that inhibition of this expression results in enhanced tumor cell killing
Gattenloehner et al., Blood 2007 (Acute Disease...) : Both p48 and CD56 induced nuclear translocation of nuclear factor (NF)-kappaB and increased bcl2L12 expression, and inhibition of this pathway by small inhibitory RNA mediated p48 knock down or NF-kappaB blockade substantially increased apoptosis in CD56 ( + ) AML cell lines
Shimizu et al., Immunology 2007 : Recently, a mycoplasma lipoprotein has been shown to induce nuclear factor kappaB (NF-kappaB) activation through toll-like receptor 1 (TLR1) , TLR2 and TLR6 ... Recently, a mycoplasma lipoprotein has been shown to induce nuclear factor kappaB (NF-kappaB) activation through toll-like receptor 1 (TLR1), TLR2 and TLR6 ... Recently, a mycoplasma lipoprotein has been shown to induce nuclear factor kappaB (NF-kappaB) activation through toll-like receptor 1 (TLR1), TLR2 and TLR6 ... Purified N-ALP1 and N-ALP2 from M. pneumoniae and triacylated partial synthetic lipopeptides of N-ALP1 and N-ALP2 augmented the levels of NF-kappaB induction through TLR1- and TLR2 dependent pathways, whereas diacylated partial synthetic lipopeptides of N-ALP1 and N-ALP2 activated NF-kappaB through TLR1-, TLR2- and TLR6 dependent pathways ... Purified N-ALP1 and N-ALP2 from M. pneumoniae and triacylated partial synthetic lipopeptides of N-ALP1 and N-ALP2 augmented the levels of NF-kappaB induction through TLR1- and TLR2 dependent pathways, whereas diacylated partial synthetic lipopeptides of N-ALP1 and N-ALP2 activated NF-kappaB through TLR1-, TLR2- and TLR6 dependent pathways
Fernández-Martínez et al., Mol Cell Endocrinol 2007 (Disease Progression...) : In fact, VIP increased the translocation of the NFkappaB p50 subunit to the nucleus and the binding of the active form to its target gene promoter, as measured by Western blotting and ELISA, respectively
Nah et al., FEBS Lett 2007 (Osteoarthritis) : We also observed that NF-kappaB is involved in AGE-BSA induced TNF-alpha
Bátkai et al., Am J Physiol Heart Circ Physiol 2007 (Inflammation...) : Anandamide dose dependently attenuated the TNF-alpha induced ICAM-1 and VCAM-1 expression, NF-kappaB activation in HCAECs, and the adhesion of monocytes to HCAECs in a CB(1)- and CB(2) dependent manner ... Anandamide dose dependently attenuated the TNF-alpha induced ICAM-1 and VCAM-1 expression, NF-kappaB activation in HCAECs, and the adhesion of monocytes to HCAECs in a CB(1)- and CB(2) dependent manner
Turner et al., Ann Surg 2007 : Bile salts induce resistance to apoptosis through NF-kappaB mediated XIAP expression ... Specific inhibition of NF-kappaB by infection with an adenoviral vector that expresses the IkappaBalpha super-repressor ( IkappaBSR ) prevented the induction of XIAP expression and the bile salt mediated resistance to apoptosis ... These results indicate that bile salts increase intestinal epithelial cell resistance to apoptosis through NF-kappaB mediated XIAP expression
Grabiner et al., Genes Dev 2007 : CARMA3 deficiency abrogates G protein coupled receptor induced NF-{kappa}B activation ... Together, our results provide the genetic evidence that CARMA3 is required for GPCR induced NF-kappaB activation
Wang et al., Mol Cell Biol 2007 : NF-kappaB regulation of YY1 inhibits skeletal myogenesis through transcriptional silencing of myofibrillar genes ... While inducers of NF-kappaB stimulated YY1 expression in multiple cell types, genetic ablation of the RelA/p65 subunit of NF-kappaB in both cultured cells and adult skeletal muscle correlated with reduced YY1 transcripts and protein ... NF-kappaB regulation of YY1 occurred at the transcriptional level, mediated by direct binding of the p50/p65 heterodimer complex to the YY1 promoter ... Based on these results, we propose that NF-kappaB regulation of YY1 and transcriptional silencing of myofibrillar genes represent a new mechanism by which NF-kappaB functions in myoblasts to modulate skeletal muscle differentiation
Pandey et al., J Biol Chem 2007 : As examined by DNA binding, we found that butein suppressed tumor necrosis factor (TNF) induced NF-kappaB activation in a dose- and time dependent manner ; suppressed the NF-kappaB activation induced by various inflammatory agents and carcinogens ; and inhibited the NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2, NIK, TAK1/TAB1, and IKK-beta ... As examined by DNA binding, we found that butein suppressed tumor necrosis factor (TNF) induced NF-kappaB activation in a dose- and time dependent manner ; suppressed the NF-kappaB activation induced by various inflammatory agents and carcinogens ; and inhibited the NF-kappaB reporter activity induced by TNFR1 , TRADD, TRAF2, NIK, TAK1/TAB1, and IKK-beta ... As examined by DNA binding, we found that butein suppressed tumor necrosis factor (TNF) induced NF-kappaB activation in a dose- and time dependent manner ; suppressed the NF-kappaB activation induced by various inflammatory agents and carcinogens ; and inhibited the NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2, NIK, TAK1/TAB1 , and IKK-beta ... As examined by DNA binding, we found that butein suppressed tumor necrosis factor (TNF) induced NF-kappaB activation in a dose- and time dependent manner ; suppressed the NF-kappaB activation induced by various inflammatory agents and carcinogens ; and inhibited the NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2, NIK, TAK1/TAB1, and IKK-beta ... As examined by DNA binding, we found that butein suppressed tumor necrosis factor (TNF) induced NF-kappaB activation in a dose- and time dependent manner ; suppressed the NF-kappaB activation induced by various inflammatory agents and carcinogens ; and inhibited the NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2, NIK, TAK1/TAB1 , and IKK-beta
Kunnumakkara et al., Cancer Res 2007 (Neovascularization, Pathologic...) : In vivo, tumors from nude mice injected with pancreatic cancer cells and treated with a combination of curcumin and gemcitabine showed significant reductions in volume ( P = 0.008 versus control ; P = 0.036 versus gemcitabine alone ), Ki-67 proliferation index ( P = 0.030 versus control ), NF-kappaB activation , and expression of NF-kappaB regulated gene products ( cyclin D1, c-myc, Bcl-2, Bcl-xL , cellular inhibitor of apoptosis protein-1, cyclooxygenase-2, matrix metalloproteinase, and vascular endothelial growth factor ) compared with tumors from control mice treated with olive oil only ... In vivo, tumors from nude mice injected with pancreatic cancer cells and treated with a combination of curcumin and gemcitabine showed significant reductions in volume ( P = 0.008 versus control ; P = 0.036 versus gemcitabine alone ), Ki-67 proliferation index ( P = 0.030 versus control ), NF-kappaB activation , and expression of NF-kappaB regulated gene products ( cyclin D1, c-myc, Bcl-2, Bcl-xL, cellular inhibitor of apoptosis protein-1, cyclooxygenase-2, matrix metalloproteinase, and vascular endothelial growth factor ) compared with tumors from control mice treated with olive oil only ... In vivo, tumors from nude mice injected with pancreatic cancer cells and treated with a combination of curcumin and gemcitabine showed significant reductions in volume ( P = 0.008 versus control ; P = 0.036 versus gemcitabine alone ), Ki-67 proliferation index ( P = 0.030 versus control ), NF-kappaB activation , and expression of NF-kappaB regulated gene products ( cyclin D1 , c-myc, Bcl-2, Bcl-xL, cellular inhibitor of apoptosis protein-1, cyclooxygenase-2, matrix metalloproteinase, and vascular endothelial growth factor ) compared with tumors from control mice treated with olive oil only ... In vivo, tumors from nude mice injected with pancreatic cancer cells and treated with a combination of curcumin and gemcitabine showed significant reductions in volume ( P = 0.008 versus control ; P = 0.036 versus gemcitabine alone ), Ki-67 proliferation index ( P = 0.030 versus control ), NF-kappaB activation , and expression of NF-kappaB regulated gene products ( cyclin D1, c-myc, Bcl-2, Bcl-xL, cellular inhibitor of apoptosis protein-1 , cyclooxygenase-2, matrix metalloproteinase, and vascular endothelial growth factor ) compared with tumors from control mice treated with olive oil only ... In vivo, tumors from nude mice injected with pancreatic cancer cells and treated with a combination of curcumin and gemcitabine showed significant reductions in volume ( P = 0.008 versus control ; P = 0.036 versus gemcitabine alone ), Ki-67 proliferation index ( P = 0.030 versus control ), NF-kappaB activation , and expression of NF-kappaB regulated gene products ( cyclin D1, c-myc, Bcl-2, Bcl-xL, cellular inhibitor of apoptosis protein-1, cyclooxygenase-2 , matrix metalloproteinase, and vascular endothelial growth factor ) compared with tumors from control mice treated with olive oil only ... In vivo, tumors from nude mice injected with pancreatic cancer cells and treated with a combination of curcumin and gemcitabine showed significant reductions in volume ( P = 0.008 versus control ; P = 0.036 versus gemcitabine alone ), Ki-67 proliferation index ( P = 0.030 versus control ), NF-kappaB activation, and expression of NF-kappaB regulated gene products ( cyclin D1, c-myc, Bcl-2 , Bcl-xL, cellular inhibitor of apoptosis protein-1, cyclooxygenase-2, matrix metalloproteinase, and vascular endothelial growth factor ) compared with tumors from control mice treated with olive oil only
Rafi et al., Mol Nutr Food Res 2007 : In addition, LPS induced activation of nuclear factor-kappaB (NF-kappaB) DNA binding suggests an inhibitory effect of GLN
Geraghty et al., J Immunol 2007 (Pseudomonas Infections) : We also demonstrate that NE can activate NF-kappaB in macrophages, and inhibition of NF-kappaB resulted in a reduction of NE-induced cathepsin B and MMP-2 ... We also demonstrate that NE can activate NF-kappaB in macrophages, and inhibition of NF-kappaB resulted in a reduction of NE-induced cathepsin B and MMP-2
Caruso et al., J Immunol 2007 (Gastritis...) : Analysis of signaling pathways leading to MMP production reveals that IL-21 enhances NF-kappaB but not MAPK activation, and inhibition of NF-kappaB activation reduces IL-21 induced MMP-2 and MMP-9 production ... Analysis of signaling pathways leading to MMP production reveals that IL-21 enhances NF-kappaB but not MAPK activation, and inhibition of NF-kappaB activation reduces IL-21 induced MMP-2 and MMP-9 production ... Analysis of signaling pathways leading to MMP production reveals that IL-21 enhances NF-kappaB but not MAPK activation, and inhibition of NF-kappaB activation reduces IL-21 induced MMP-2 and MMP-9 production
Yu et al., J Cell Physiol 2007 : TNF reduces LIF endocytosis despite increasing NFkappaB mediated gp130 expression
García-Román et al., Journal of carcinogenesis 2007 : We propose that during liver regeneration, the transitory stimulation of the transcription factor response, assures blockade of NF-kappaB until recovery of the total mass of the liver and the persistent NF-kappaB activation in early hepatocarcinogenesis may be due to IkappaB-beta and IkappaB-alpha degradation, mainly IkappaB-beta degradation, which contributes to gene transcription related to proliferation required for neoplastic progression ... We propose that during liver regeneration, the transitory stimulation of the transcription factor response, assures blockade of NF-kappaB until recovery of the total mass of the liver and the persistent NF-kappaB activation in early hepatocarcinogenesis may be due to IkappaB-beta and IkappaB-alpha degradation, mainly IkappaB-beta degradation, which contributes to gene transcription related to proliferation required for neoplastic progression
Yun et al., Biochem Biophys Res Commun 2007 (Osteoarthritis) : NF-kappaB regulates Lef1 gene expression in chondrocytes ... In this study we found overexpression of Lef1 in cartilage tissue of osteoarthritic patients and elucidated molecular mechanism of NF-kappaB mediated Lef1 gene regulation in chondrocytes ... Our results suggest a pivotal role of NF-kappaB in Lef1 expression in arthritic chondrocytes or cartilage degeneration
Hung et al., Biochem Pharmacol 2007 : CCL-34 activated NF-kappaB in a TLR4 dependent manner and stimulated TNF-alpha production in bone marrow cells of TLR4-functional C3H/HeN mice but not in those of TLR4-defective C3H/HeJ mice ... CCL-34 activated NF-kappaB in a TLR4 dependent manner and stimulated TNF-alpha production in bone marrow cells of TLR4-functional C3H/HeN mice but not in those of TLR4-defective C3H/HeJ mice
Matsuo et al., Biochem J 2007 : The activation of the NGAL promoter by transfection of IkappaB-zeta and NF-kappaB was suppressed in C/EBPbeta depleted cells
Nita et al., Int J Biochem Cell Biol 2007 : In parallel, a short-term ( 2h ) cell exposure to AAT/LPS significantly enhances LPS induced NF kappaB ( p50 and p65 ) activation in conjunction with increased TNFalpha, IL-1 beta and IL-8 release
Tian et al., J Biol Chem 2007 : RBCK1 negatively regulates tumor necrosis factor- and interleukin-1 triggered NF-kappaB activation by targeting TAB2/3 for degradation ... RBCK1 negatively regulates tumor necrosis factor- and interleukin-1 triggered NF-kappaB activation by targeting TAB2/3 for degradation ... The NZF domains of TAB2/3 are critical for TAB2/3 to bind to Lys ( 63 ) -linked polyubiquitin chains of other adaptor proteins, such as receptor interacting protein and TRAF6, which are two signaling proteins essential for TNF- and IL-1 induced NF-kappaB activation, respectively ... Overexpression of RBCK1 negatively regulates TAB2/3 mediated and TNF- and IL-1 induced NF-kappaB activation, whereas knockdown of RBCK1 by RNA interference potentiates TNF- and IL-1 induced NF-kappaB activation ... Overexpression of RBCK1 negatively regulates TAB2/3 mediated and TNF- and IL-1 induced NF-kappaB activation, whereas knockdown of RBCK1 by RNA interference potentiates TNF- and IL-1 induced NF-kappaB activation
Ruiz et al., J Nutr 2007 (Ileitis) : Quercetin inhibits TNF induced NF-kappaB transcription factor recruitment to proinflammatory gene promoters in murine intestinal epithelial cells
Moon et al., Infect Immun 2007 (Acute Disease...) : Toll-like receptor 2-dependent NF-kappaB activation is involved in nontypeable Haemophilus influenzae induced monocyte chemotactic protein 1 up-regulation in the spiral ligament fibrocytes of the inner ear ... Here we show for the first time that NTHI induces MCP-1 up-regulation in the SLFs via Toll-like receptor 2 (TLR2) dependent activation of NF-kappaB
Cude et al., J Cell Biol 2007 : Stimulation of ERK5 activated nuclear factor kappaB (NFkappaB) through ribosomal S6 kinase 2 (RSK2) mediated phosphorylation and degradation of IkappaB ... Furthermore, selective inhibition of NFkappaB at G2-M phases substantially delayed mitotic entry and inhibited transcription of G2-M-specific genes, including cyclin B1 , cyclin B2, Plk-1, and cdc25B ... Furthermore, selective inhibition of NFkappaB at G2-M phases substantially delayed mitotic entry and inhibited transcription of G2-M-specific genes, including cyclin B1, cyclin B2 , Plk-1, and cdc25B ... Furthermore, selective inhibition of NFkappaB at G2-M phases substantially delayed mitotic entry and inhibited transcription of G2-M-specific genes, including cyclin B1, cyclin B2, Plk-1 , and cdc25B
Lee et al., Cancer Lett 2007 (Colonic Neoplasms) : The results obtained from electrophoretic mobility shift assay ( EMSA ) and Western blot analysis showed that UDN glycoprotein blocks the DNA binding activities of nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1), and attenuates the activities of NF-kappaB subunits ( p50 and p65 ), and AP-1 subunits ( c-Jun and c-Fos ), respectively
Bohren et al., Protein Expr Purif 2007 : The recently identified SUMO4 was detected in mRNA transcripts from HEK293 cells, and human kidney and spleen tissue and may be involved in regulation of NF-kappaB and susceptibility to autoimmune diseases
Gerstung et al., Am J Nephrol 2007 (Inflammation) : Endothelin-1 induces NF-kappaB via two independent pathways in human renal tubular epithelial cells
Fang et al., Intervirology 2007 : One of the scFv clones, designated H3, was capable of reducing LMP1 mediated NF-kappaB activation in HEK293 cells
Ock et al., J Neurosci Res 2007 (Inflammation) : Hypoxia, however, differentially regulated MyD88 dependent and -independent pathways of TLR4 signaling : Hypoxia enhanced lipopolysaccharide (LPS) induced interferon regulatory factor-3 (IRF-3) activation and the subsequent expression of IFNbeta ( MyD88 independent pathway ), whereas it suppressed LPS induced NF-kappaB activation ( MyD88 dependent pathway ) ... Hypoxia, however, differentially regulated MyD88 dependent and -independent pathways of TLR4 signaling : Hypoxia enhanced lipopolysaccharide (LPS) induced interferon regulatory factor-3 (IRF-3) activation and the subsequent expression of IFNbeta ( MyD88 independent pathway ), whereas it suppressed LPS induced NF-kappaB activation ( MyD88 dependent pathway ) ... Hypoxia, however, differentially regulated MyD88 dependent and -independent pathways of TLR4 signaling : Hypoxia enhanced lipopolysaccharide (LPS) induced interferon regulatory factor-3 (IRF-3) activation and the subsequent expression of IFNbeta ( MyD88 independent pathway ), whereas it suppressed LPS induced NF-kappaB activation ( MyD88 dependent pathway )
Xu et al., World J Gastroenterol 2007 (Acute Disease...) : The beneficial effect of pioglitazone is multifactorial due to its anti-inflammatory activities, most likely through the inhibition of ICAM-1 expression and NF-kappaB activation
San Martin et al., Free Radic Biol Med 2007 (Vascular Diseases) : AGE stimulated NF-kappaB activity was abolished by DPI and the superoxide scavenger MnTBAP
He et al., Cell Signal 2007 : NF-kappaB inhibition enhances caspase-3 degradation of Akt1 and apoptosis in response to camptothecin ... NF-kappaB inhibition enhances caspase-3 degradation of Akt1 and apoptosis in response to camptothecin ... While CPT induced NF-kappaB activation could not be detected in ATM-deficient AT5BIVA cells, caspase-3 activation occurred and was even further enhanced by pretreatment with proteasome inhibitor-1 (Pro1) , a NF-kappaB inhibitor ... NF-kappaB inhibition by Pro1, dominant negative mutant IkappaBalpha ( S32/36 ) or p65 ( N250 ), however, induced the caspase-3 activation in the normal cells, indicating the role of ATM mediated NF-kappaB activation against cell apoptosis
Gringhuis et al., Immunity 2007 : We demonstrated that pathogens trigger DC-SIGN on human DCs to activate the serine and threonine kinase Raf-1, which subsequently leads to acetylation of the NF-kappaB subunit p65, but only after TLR induced activation of NF-kappaB
Misra et al., J Biol Chem 2007 (Alstrom Syndrome) : Consistent with this observation, inhibition of caspase activity attenuates NF-kappaB activation
Lim et al., Exp Mol Med 2007 : Toll-like receptor 9 dependent activation of MAPK and NF-kB is required for the CpG ODN induced matrix metalloproteinase-9 expression
Plesnila et al., Cell Death Differ 2007 (Brain Injuries...) : Delayed neuronal death after brain trauma involves p53 dependent inhibition of NF-kappaB transcriptional activity ... Inhibition of p53 activity resulted in the concomitant increase in NF-kappaB transcriptional activity and upregulation of NF-kappaB-target proteins, for example X-chromosomal linked inhibitor of apoptosis ( XIAP )
Lei et al., Annu Rev Nutr 2007 (Chronic Disease) : Intracellular and tissue levels of GPX1 activity affect apoptotic signaling pathway, protein kinase phosphorylation, and oxidant mediated activation of NFkappaB
Lee et al., Int Immunopharmacol 2007 : d-pinitol was also demonstrated to inhibit LPS induced MAPKs activation and NF-kappaB nuclear translocation
Tsou et al., Toxicol Appl Pharmacol 2007 : BSO attenuated the TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation, however, with no detectable effect on AP-1 and its related mitogen activated protein kinases ( MAPKs ) ... Inhibition of ERK, JNK, or NF-kappaB attenuates TNF-alpha induced ICAM-1 promoter activation and monocyte-endothelial monolayer binding
Laine et al., Biochem Biophys Res Commun 2007 : PA also induced an approximately 2-fold increase ( p < 0.05 ) in active NF-kappaB, and two structurally distinct NF-kappaB inhibitors effectively blocked PA-induced IP-10 gene expression ... These results suggest that elevated concentrations of PA commonly present in obese and insulin resistant individuals can increase NF-kappaB mediated expression of IP-10 in macrophages
Xie et al., Am J Physiol Lung Cell Mol Physiol 2007 (Asthma...) : The mitogenic activity of TGF-beta1 on ASM cells was inhibited by selective inhibitors of TGF-beta receptor I kinase ( SD-208 ), phosphatidylinositol 3-kinase ( PI3K, LY-294002 ), ERK ( PD-98059 ), JNK ( SP-600125 ), and NF-kappaB ( AS-602868 ) ... NF-kappaB is also involved in the TGF-beta1 mitogenic signaling, but Smad pathway does not appear important
Agouni et al., Carcinogenesis 2007 (Carcinoma, Renal Cell...) : Using specific inhibitors, western blot and transcription assay, we identified nuclear factor kappa B (NF-kappaB) as the downstream Akt target regulated by PTHrP
Tanaka et al., Nat Immunol 2007 : Here we report that PDLIM2 negatively regulated NF-kappaB activity, acting as a nuclear ubiquitin E3 ligase targeting the p65 subunit of NF-kappaB
Medeiros et al., Br J Pharmacol 2007 (Edema) : Treatment with either alpha-humulene or trans-caryophyllene effectively reduced neutrophil migration and activation of NF-kappaB induced by LPS in the rat paw ... Treatment with either alpha-humulene or trans-caryophyllene effectively reduced neutrophil migration and activation of NF-kappaB induced by LPS in the rat paw ... Both alpha-humulene and trans-caryophyllene inhibit the LPS induced NF-kappaB activation and neutrophil migration, although only alpha-humulene had the ability to prevent the production of pro-inflammatory cytokines TNF-alpha and IL-1beta and the in vivo up-regulation of kinin B ( 1 ) receptors
Azoitei et al., Oncogene 2007 : Here, we report that expression and consequent activation of TNK1 enables tumor necrosis factor alpha (TNFalpha) induced apoptosis by selectively inhibiting TNFalpha induced activation of nuclear factor-kappaB (NF-kappaB) ... TNK1 has no effect on NF-kappaB DNA binding or the composition of the NF-kappaB complex ; however, the kinase markedly prevents TNFalpha induced NF-kappaB transactivation
Xiao et al., Mol Pharmacol 2007 : The effects of 5-AED on survival and G-CSF secretion were blocked by the NFkappaB inhibitor N-benzoyloxycarbonyl ( Z ) -Leu-Leu-leucinal ( MG132 )
Wegener et al., Science's STKE : signal transduction knowledge environment 2007 : CARD11 ( CARMA1 ), Bcl10, and Malt1 are required for nuclear factor NF-kappaB activation in response to antigen recognition ... CARD11 ( CARMA1 ), Bcl10, and Malt1 are required for nuclear factor NF-kappaB activation in response to antigen recognition ... CARD11 ( CARMA1 ), Bcl10 , and Malt1 are required for nuclear factor NF-kappaB activation in response to antigen recognition ... The CARD10 ( CARMA3 ) -Bcl10-Malt1 signalosome functions as a link between G protein coupled receptor ( GPCR ) signaling and proinflammatory NF-kappaB activation
Chung et al., Biol Pharm Bull 2007 : The LPS/rIFN-gamma induced activation of NF-kappaB was almost completely blocked by MC at 0.5 mg/ml ... These findings demonstrate that the inhibition of the LPS/rIFN-gamma induced production of NO and TNF-alpha by MC is due to the inhibition of NF-kappaB activation
Sugiura et al., Nihon Rinsho Meneki Gakkai Kaishi 2007 (Arthritis...) : Mutations of cyropyrin lead to the persistent production of IL-1beta and activation of NF-kappaB , followed by excessive inflammtory reactions
Lee et al., J Mol Biol 2007 : In STAT1-deficient mice, nuclear factor kappaB (NF-kappaB) activation by TNF-alpha/IFN-gamma was attenuated and strongly inhibited by both NAC and L-NMMA ... In STAT1-deficient mice, nuclear factor kappaB (NF-kappaB) activation by TNF-alpha/IFN-gamma was attenuated and strongly inhibited by both NAC and L-NMMA ... Moreover, the proteasome inhibitor, MG132, inhibited NF-kappaB activation and strongly inhibited iNOS/NO induction, ROS production, and loss of DeltaPsim induced by TNF-alpha/IFN-gamma, thereby inhibiting apoptosis
Karrasch et al., J Immunol 2007 (Colitis...) : We conclude that bacteria induced experimental colitis involves the activation of TLR induced NF-kappaB signaling derived mostly from mucosal immune cells ... Blocking TLR induced NF-kappaB activity may represent an attractive strategy to treat immune mediated intestinal inflammation
Simon et al., J Interferon Cytokine Res 2007 : WT and dam ( - ) strains displayed a similar Toll-like receptor 5 (TLR5) dependent NF-kappaB activation, whereas the flhC ( - ) mutant lacked this activity
Lim et al., Life Sci 2007 : Since p38 MAPK can activate NF-kappaB , we made point mutations in the NF-kappaB binding site within the COX-2 promoter ... Thus, induction of COX-2 expression in M-1 cells by hypertonic sodium chloride does not involve activation of NF-kappaB
Medeiros et al., Science 2007 : These findings provide new insights into ADAP function and the mechanism by which CARMA1 regulates NF-kappaB activation in T cells
Guo et al., Zhong Nan Da Xue Xue Bao Yi Xue Ban 2007 : The inhibition of ERK can suppress the DNA binding activity of NF-kappaB and the cell proliferation mediated by HCV NS3 protein in a dose dependent manner
Bezdecny et al., Toxicol Appl Pharmacol 2007 : 2,2',4,4'-Tetrachlorobiphenyl upregulates cyclooxygenase-2 in HL-60 cells via p38 mitogen activated protein kinase and NF-kappaB
Tas et al., Blood 2007 (Inflammation) : Noncanonical NF-kappaB signaling in dendritic cells is required for indoleamine 2,3-dioxygenase (IDO) induction and immune regulation ... By selective blocking of either the canonical NF-kappaB pathway using the NEMO binding domain peptide or the noncanonical NF-kappaB pathway by small interfering RNA, we demonstrate that IDO expression requires noncanonical NF-kappaB signaling
Chang et al., Mol Immunol 2007 : TSA1 could also inhibit TNF induced cytotoxicity on L929 cells and TNF mediated NF-kappaB activation on HEK-293T cells ... TSA1 could also inhibit TNF induced cytotoxicity on L929 cells and TNF mediated NF-kappaB activation on HEK-293T cells
Varela-Rey et al., Cytokine 2007 : Activation of NFkappaB by TNF-alpha was also abolished by preincubation of HSC with GSH, but not by deferoxamine, tempol or trolox
Yamashita et al., J Biol Chem 2007 : NF-kappaB p50 and p52 regulate receptor activator of NF-kappaB ligand ( RANKL ) and tumor necrosis factor induced osteoclast precursor differentiation by activating c-Fos and NFATc1 ... NF-kappaB p50 and p52 regulate receptor activator of NF-kappaB ligand ( RANKL ) and tumor necrosis factor induced osteoclast precursor differentiation by activating c-Fos and NFATc1 ... Inhibition of NF-kappaB should prevent RANKL- and TNF induced bone resorption
Wang et al., J Gen Virol 2007 (MAP Kinase Signaling System) : Similarly to K15-P, K15-M induces the activation of the Erk2 and JNK1 kinases, the NF-kappaB transcription factor and the expression of a similar range of cellular inflammatory genes, as assessed by gene-expression microarray studies and reporter assays
Wahyudi et al., Acta Med Indones 2007 (Arteriosclerosis) : Green tea polyphenols inhibit oxidized LDL induced NF-KB activation in human umbilical vein endothelial cells ... We postulate that green tea polyphenols regulate TNF-alpha gene expression by modulating NF-KB activation through their inhibition effect on IKB Kinase (IKK) activity and as scavenger of free radicals
Jung et al., Biosci Biotechnol Biochem 2007 : In addition, it blocked LPS stimulated activation of nuclear factor kappa B (NF-kappaB)
Tsuruki et al., Biosci Biotechnol Biochem 2007 (Alopecia) : Hence, we suggest that the anti-alopecia mechanisms of intraperitoneally administered fMLP and MMK-1 include activation of NF-kappaB via IL-1 release downstream of the FPRL1 receptor homolog in rats
Pathak et al., Nat Immunol 2007 : Here we show that ESAT-6 inhibited activation of transcription factor NF-kappaB and interferon-regulatory factors ( IRFs ) after Toll-like receptor ( TLR ) signaling ; inhibition of TLR signaling by ESAT-6 required the kinase Akt
Hara et al., Nat Immunol 2007 (Listeriosis) : The CARMA1-Bcl-10-MALT1 complex is critical for the activation of transcription factor NF-kappaB in lymphocytes but has an unclear function in myeloid cells ... The CARMA1-Bcl-10-MALT1 complex is critical for the activation of transcription factor NF-kappaB in lymphocytes but has an unclear function in myeloid cells
Xue et al., Ann Rheum Dis 2007 (Activated Protein C Resistance...) : APC also completely suppressed LPS stimulated NF-kappaB activation and attenuated TNF-alpha protein by more than 40 % in RA monocytes ... APC also completely suppressed LPS stimulated NF-kappaB activation and attenuated TNF-alpha protein by more than 40 % in RA monocytes
Sanada et al., J Clin Invest 2007 : Although IL-33 activated NF-kappaB , it inhibited angiotensin II- and phenylephrine induced phosphorylation of inhibitor of NF-kappa B alpha ( I kappa B alpha ) and NF-kappaB nuclear binding activity
Ueda et al., Melanoma Res 2007 (Melanoma...) : We observed that proteins expressed by Hs294T metastatic melanoma cells are highly acetylated compared with normal melanocytes, and dominant negative PCAF reduced the basal and tumor necrosis factor-alpha stimulated transcriptional activity of NF-kappaB ... We observed that proteins expressed by Hs294T metastatic melanoma cells are highly acetylated compared with normal melanocytes, and dominant negative PCAF reduced the basal and tumor necrosis factor-alpha stimulated transcriptional activity of NF-kappaB
Park et al., Int Immunopharmacol 2007 (Inflammation) : Molecular mechanisms that govern attenuation of the levels of mRNAs and proteins of these cytokines and iNOS revealed that the PC extract inhibited LPS stimulated phosphorylation of ERK and activation of NF-kappaB ... Molecular mechanisms that govern attenuation of the levels of mRNAs and proteins of these cytokines and iNOS revealed that the PC extract inhibited LPS stimulated phosphorylation of ERK and activation of NF-kappaB
Ho et al., Biochem Pharmacol 2007 : Inhibitory effect on IL-1beta mediated NF-kappaB activation was evidenced by the diminishment of IkappaB kinase (IKK) phosphorylation and IkappaBalpha degradation
Lambert et al., J Bone Miner Res 2007 : In contrast, NF-kappaB was not essential for IL-1beta induction of OPG ... In contrast, NF-kappaB was not essential for IL-1beta induction of OPG
Peng et al., Cytokine 2007 : RKC1 and RKC2 are similar to primary Kupffer cells as they express the molecular markers ED1, ED2, ED3, and F4/80, and upregulate TNF-alpha, IL-6, IL-1beta, Fas /FasL, and NF-kappaB , as well as TLR4 in response to LPS or pancreatic elastase
Hu et al., J Leukoc Biol 2007 : Similarly, basal ITAM signaling augments IFN signaling and function of receptor activator of NF-kappaB , but extensive ITAM activation inhibits Jak-STAT signaling ... Similarly, basal ITAM signaling augments IFN signaling and function of receptor activator of NF-kappaB , but extensive ITAM activation inhibits Jak-STAT signaling
Zeng et al., Mol Cell Biol 2007 : Phosphorylation of Bcl10 negatively regulates T-cell receptor mediated NF-kappaB activation ... Bcl10 plays a critical role in antigen receptor mediated NF-kappaB activation and lymphocyte development and functions
Martinez-Losa et al., Eur Respir J 2007 : The increase in eosinophil NF-kappaB binding activity induced by GM-CSF and TNF-alpha was suppressed by NAC ... The increase in eosinophil NF-kappaB binding activity induced by GM-CSF and TNF-alpha was suppressed by NAC ... The increase in eosinophil NF-kappaB binding activity induced by GM-CSF and TNF-alpha was suppressed by NAC
Kim et al., Mol Pharmacol 2007 (Calcium Signaling) : Subsequent characterization of the DMS mediated suppression of osteoclastogenesis revealed that DMS did not affect RANKL induced activation of JNK, p38, NF-kappaB , and Ca2+ oscillation
Li et al., Shock 2007 : We found that the pretreatment of cells with CCK-8 blocked the LPS induced p38 kinase, NF-kappaB activation, and IL-1beta production ... Furthermore, CCK-8 treatment activated the cyclic adenosine monophosphate-protein kinase A signaling pathway and H-89 ( a protein kinase A inhibitor ), abrogated the inhibitory effects of CCK-8 on p38 kinase activation and NF-kappaB activation ... Taken together, our results indicate that the stimulation of cyclic adenosine monophosphate-protein kinase A signaling pathway by CCK-8 through CCK-1R and CCK-2R inhibits the LPS induced activation of p38 kinase and NF-kappaB to block the IL-1beta production in rat pulmonary interstitial macrophages ... Taken together, our results indicate that the stimulation of cyclic adenosine monophosphate-protein kinase A signaling pathway by CCK-8 through CCK-1R and CCK-2R inhibits the LPS induced activation of p38 kinase and NF-kappaB to block the IL-1beta production in rat pulmonary interstitial macrophages
Browatzki et al., Eur J Med Res 2007 : Experiments with PI-1, an inhibitor of the IkappaB-a degrading proteasome complex, demonstrated involvement of the transcription factor NF-kappaB in ET-1 induced CD40 expression
Lee et al., J Leukoc Biol 2007 : IFN-gamma induced CD40 expression involves activation of STAT-1alpha as well as NF-kappaB activation through an autocrine response to IFN-gamma induced TNF-alpha production ... IFN-gamma induced CD40 expression involves activation of STAT-1alpha as well as NF-kappaB activation through an autocrine response to IFN-gamma induced TNF-alpha production
Jin et al., Mol Cancer Res 2007 (Neoplasm Invasiveness...) : Further studies showed that p75NTR could suppress the nuclear factor-kappaB (NF-kappaB) signal ... SN50, a specific inhibitor of NF-kappaB , which could inhibit in vitro invasive and migratory abilities of gastric cancer cells, reduced expression of uPA and MMP9 proteins and increased expression of TIMP1 protein ... SN50, a specific inhibitor of NF-kappaB , which could inhibit in vitro invasive and migratory abilities of gastric cancer cells, reduced expression of uPA and MMP9 proteins and increased expression of TIMP1 protein ... SN50, a specific inhibitor of NF-kappaB , which could inhibit in vitro invasive and migratory abilities of gastric cancer cells, reduced expression of uPA and MMP9 proteins and increased expression of TIMP1 protein
Kawamura et al., Arterioscler Thromb Vasc Biol 2007 (Atherosclerosis) : Studies in VSMCs overexpressing IL-1beta signaling intermediates revealed that NF-kappaB transactivation was inhibited by apoE in MyD88- and IRAK1- but not in TRAF6 transfected cells
Yu et al., Gynecol Oncol 2007 (Lung Neoplasms...) : As ( 2 ) O ( 3 ) treatment also resulted in a positive regulation of caveolin-1, upregulation of E-cadherin and decreased activity of beta-catenin, NF-kappaB and NF-kappaB regulated gene MMP-2
Thieu et al., J Leukoc Biol 2007 : IL-4 stimulated NF-kappaB activity is required for Stat6 DNA binding ... IL-4 stimulated NF-kappaB activity is required for Stat6 DNA binding ... IL-4 induced NF-kappaB p52 production requires PI-3K activity and correlates with IkappaB kinase phosphorylation and TNF receptor associated factor 3 degradation
Hayashi et al., J Immunol 2007 (Acute Disease...) : In comparison with WT mice, IFN-gamma ( -/- ) mice exhibited exaggerated and prolonged NF-kappaB activation, probably due to reduced acetylation of Stat1, a main signal transducer of IFN-gamma, because acetylated Stat1 can inhibit NF-kappaB activation ... Indeed, IFN-gamma acetylated Stat1 and reciprocally reduced NF-kappaB activation and COX-2 expression in neutrophils
Krasnow et al., Cytokine 1991 (Lymphoma, T-Cell) : Tumor necrosis factor-alpha, interleukin 1, and phorbol myristate acetate are independent activators of NF-kappa B which differentially activate T cells ... Tumor necrosis factor-alpha , interleukin 1, and phorbol myristate acetate are independent activators of NF-kappa B which differentially activate T cells
Saban et al., BMC urology 2007 (Cystitis) : Electrophoretic mobility shift assays confirmed that, in the mouse urinary bladder, activation of NK1R by substance P ( SP ) induces both NKx-2.5 and NF-kappaB translocations
Schwab et al., Mol Immunol 2007 : Recently, butyrate has not only been demonstrated to suppress NF kappa B activation in colorectal cancer cells, but also to modulate the activity and expression of the Peroxisome-Proliferator-Activated-Receptor gamma ( PPAR gamma ) and the vitamin D receptor (VDR) ... Adversely, the inhibitory effect of butyrate on NF kappa B activity induced by LPS was almost reversed in dominant negative PPAR gamma mutant cells while pre-incubation of ZK191732 did not affect butyrate mediated attenuation of LPS induced NF kappa B signalling
Sandur et al., Carcinogenesis 2007 : The results indicate that the relative potency for suppression of tumor necrosis factor (TNF) induced nuclear factor-kappaB (NF-kappaB) activation was Cur > DMC > BDMC ; thus suggesting the critical role of methoxy groups on the phenyl ring ... Turmerones also failed to inhibit TNF induced NF-kappaB activation ... The suppression of NF-kappaB activity correlated with inhibition of NF-kappaB reporter activity and with down-regulation of cyclooxygenase-2, cyclin D1 and vascular endothelial growth factor , all regulated by NF-kappaB ... The suppression of NF-kappaB activity correlated with inhibition of NF-kappaB reporter activity and with down-regulation of cyclooxygenase-2, cyclin D1 and vascular endothelial growth factor, all regulated by NF-kappaB ... The suppression of NF-kappaB activity correlated with inhibition of NF-kappaB reporter activity and with down-regulation of cyclooxygenase-2, cyclin D1 and vascular endothelial growth factor, all regulated by NF-kappaB ... The suppression of NF-kappaB activity correlated with inhibition of NF-kappaB reporter activity and with down-regulation of cyclooxygenase-2 , cyclin D1 and vascular endothelial growth factor, all regulated by NF-kappaB
Gaudreault et al., J Virol 2007 : In this report, we demonstrate that infectious and UV-inactivated EBV virions lead to the activation of NF-kappaB through TLR2 using HEK293 cells cotransfected with TLR2 expressing vector along with NF-kappaB-Luc reporter plasmid ... NF-kappaB activation in HEK293-TLR2 cells ( HEK293 cells transfected with TLR2 ) by EBV was not enhanced by the presence of CD14
Nakabe et al., Int J Hyperthermia 2007 (Fever) : To assess the effects of hyperthermia on TNF-alpha induced up regulation of ECAM and TNF-alpha induced activation of NF-kappaB , we measured ECAM by ELISA, and evaluated the activation of NF-kappaB by Western blotting after TNF-alpha stimulation ... Pretreatment of hyperthermia blocks TNF-alpha induced NF-kappaB activation, resulting in the inhibition of ECAM up regulation in HAEC
Huber et al., Mol Hum Reprod 2007 (Endometriosis) : Electrophoretic mobility shift assay revealed that treatment of these cultures with the urinary preparation hCG-A suppressed TNF-alpha- or IL-1beta induced NF-kappaB DNA binding activity, whereas another urinary hCG preparation ( hCG-B ) was less effective ... Immunofluorescene of its p65 subunit revealed that pre-incubation with hCG-A strongly decreased TNF-alpha dependent nuclear expression of NF-kappaB
Baumann et al., J Clin Invest 2007 (Disease Models, Animal...) : IKK2 activation caused increased expression of known NF-kappaB target genes, including mediators of the inflammatory response such as TNF-alpha and ICAM-1
Amin et al., Arthritis Rheum 2007 (Arthritis, Rheumatoid) : IL-18 induced SDF-1alpha/CXCL12 up-regulation was dependent on JNK, p38 MAPK, phosphatidylinositol 3-kinase (PI3K), and NFkappaB ... Additionally, RA ST fibroblast IL-18 induced MCP-1/CCL2 production was mediated by JNK, PI3K, and NFkappaB ... IL-18 induced RA ST fibroblast production of VEGF was mediated mainly by JNK-2, PKCalpha, and NFkappaB ... IL-18 induced phosphorylation of JNK, PKCdelta, p38 MAPK, and activating transcription factor 2 ( ATF-2 ) in RA ST fibroblasts in a time dependent manner, with JNK-2 being upstream of PKCdelta, ATF-2, and NFkappaB
Wang et al., Virus Res 2007 : Further experiments showed that IL-6 and TNF-alpha production were dependent on NF-kappaB , which was activated through I-kappaBalpha degradation ... Further experiments showed that IL-6 and TNF-alpha production were dependent on NF-kappaB , which was activated through I-kappaBalpha degradation
Moon et al., Immunol Lett 2007 : Lipopolysaccharide (LPS) activates NF-kappaB through toll-like receptor 4 (TLR4) dependent signal transduction
Sethi et al., Oncogene 2007 (Adenocarcinoma...) : Overexpression of epidermal growth factor (EGF) receptor and constitutive activation of nuclear factor-kappaB (NF-kappaB) are frequently encountered in tumor cells ... EGF activated NF-kappaB DNA binding, induced NF-kappaB reporter activity and the expression of antiapoptotic and cell-proliferative gene products ... Although DN-IKKbeta inhibited TNF induced NF-kappaB activity, DN-IKKbeta had no effect on EGF induced NF-kappaB activation, suggesting that EGF induced NF-kappaB activation is IKK independent ... Although DN-IKKbeta inhibited TNF induced NF-kappaB activity, DN-IKKbeta had no effect on EGF induced NF-kappaB activation, suggesting that EGF induced NF-kappaB activation is IKK independent ... Although DN-IKKbeta inhibited TNF induced NF-kappaB activity, DN-IKKbeta had no effect on EGF induced NF-kappaB activation, suggesting that EGF induced NF-kappaB activation is IKK independent ... Using dominant negative plasmids, we also demonstrated the role of TRADD , TRAF2, NIK and Ras in EGF induced NF-kappaB activation ... Using dominant negative plasmids, we also demonstrated the role of TRADD, TRAF2 , NIK and Ras in EGF induced NF-kappaB activation ... Using dominant negative plasmids, we also demonstrated the role of TRADD, TRAF2, NIK and Ras in EGF induced NF-kappaB activation ... Furthermore, EGF receptor kinase inhibitor blocked IkappaBalpha phosphorylation and consequent NF-kappaB activation
Merling et al., Retrovirology 2007 : HTLV-1 Tax is a potent activator of viral transcription and NF-kappaB
N'Guessan et al., Eur Respir J 2007 : Moraxella catarrhalis induces ERK- and NF-kappaB dependent COX-2 and prostaglandin E2 in lung epithelium
Meng et al., Am J Physiol Cell Physiol 2007 (Liver Neoplasms, Experimental...) : Moreover, Akt was involved in NF-kappaB dependent VEGF expression and angiogenesis
Gauss et al., J Leukoc Biol 2007 : Role of NF-kappaB in transcriptional regulation of the phagocyte NADPH oxidase by tumor necrosis factor-alpha ... Pharmacological inhibitors of NF-kappaB activation blocked TNF-alpha induced up-regulation of NCF1, NCF2, and CYBB message, which correlated with a reduction in expression of the corresponding oxidase proteins and decreased O2*- production ... Pharmacological inhibitors of NF-kappaB activation blocked TNF-alpha induced up-regulation of NCF1 , NCF2, and CYBB message, which correlated with a reduction in expression of the corresponding oxidase proteins and decreased O2*- production ... Pharmacological inhibitors of NF-kappaB activation blocked TNF-alpha induced up-regulation of NCF1, NCF2 , and CYBB message, which correlated with a reduction in expression of the corresponding oxidase proteins and decreased O2*- production ... This novel finding supports a model, whereby TNF-alpha dependent activation of NF-kappaB up-regulates phagocyte NADPH oxidase activity, leading to enhanced ROS production and further NF-kappaB activation, potentially contributing to sustained oxidant production in chronic inflammation
Pokharel et al., Biol Pharm Bull 2007 : The lignan suppressed the reporter gene activity and the LPS induced reporter activations of nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) were also significantly blocked by 4-hydroxykobusin
Itoh et al., Biol Pharm Bull 2007 (Arthritis, Rheumatoid) : DHT inhibited the NF-kappaB activation induced by TNFalpha in a manner dependent on the androgen receptor (AR) ... These results suggest that DHT inhibits the TNFalpha induced IL-1alpha mRNA expression by inhibiting NF-kappaB activation, and contributes to the gender differences of the disease
Wu et al., J Cell Physiol 2008 (Glioblastoma...) : LRRC4 expression significantly inhibited the expression of some cytokines and their receptors determined by microarray and Western blot assays, and dramatically reduced cytokine induced AP-1, NF-kB , and CyclinD1 activation in glioma cells
Ren et al., J Agric Food Chem 2007 : To explore the mechanisms associated with the inhibition of iNOS gene expression by ALA, we investigated its effect on LPS induced nuclear factor-kappaB (NF-kappaB) activation
Fillmann et al., Toxicology 2007 (Colitis...) : We investigated the effects of glutamine on markers of oxidative stress, nuclear factor kappaB (NF-kappaB) activation, and pro-inflammatory mediators in a rat model of experimental colitis induced by intracolonic administration of 7 % acetic acid
Albrecht et al., Cell Signal 2007 : Activation of NF-kappaB by IL-1beta blocks IL-6 induced sustained STAT3 activation and STAT3 dependent gene expression of the human gamma-fibrinogen gene ... Activation of NF-kappaB by IL-1beta blocks IL-6 induced sustained STAT3 activation and STAT3 dependent gene expression of the human gamma-fibrinogen gene ... In summary, our results indicate that expression of the gamma-fibrinogen gene is mainly controlled by the strength of late phase STAT3 activation, which in turn is negatively regulated by the extent of IL-1beta mediated NF-kappaB activity
Legarda-Addison et al., J Immunol 2007 : Consistent with this hypothesis, ectopic expression of p100 inhibited TCR mediated NF-kappaB activity and IL-2 production in Jurkat T cells
Gobert et al., J Immunol 2007 : Consequently, the NF-kappaB dependent CCL20 and IL-8 gene transcription and chemokine production were enhanced in T84 cells infected with the Stx mutant in comparison to the wild-type strain ... Consequently, the NF-kappaB dependent CCL20 and IL-8 gene transcription and chemokine production were enhanced in T84 cells infected with the Stx mutant in comparison to the wild-type strain
Rosenstiel et al., J Immunol 2007 (Crohn Disease) : We show that DMBT1 inhibits cytoinvasion of Salmonella enterica and LPS- and muramyl dipeptide induced NF-kappaB activation and cytokine secretion in vitro
Loiarro et al., J Leukoc Biol 2007 (Chronic Disease...) : Moreover, ST2825 interfered with recruitment of IRAK1 and IRAK4 by MyD88, causing inhibition of IL-1beta mediated activation of NF-kappaB transcriptional activity
Deepak et al., Clin Exp Immunol 2007 (Lymphoma, T-Cell...) : The expression of iNOS and proinflammatory cytokines are dependent largely upon the activation of nuclear factor-kappaB (NF-kappaB) ... Activation of NF-kappaB involves the degradation of cytoplasmic inhibitor IkappaBalpha , allowing the nuclear translocation of NF-kappaB and thereby transcription of the iNOS gene
Zhu et al., Dermatol Surg 2007 (Keloid) : Abnormal nuclear factor (NF)-kappaB signal pathway and aspirin inhibits tumor necrosis factor alpha induced NF-kappaB activation in keloid fibroblasts ... To examine the effect of aspirin on the tumor necrosis factor (TNF)-alpha induced NF-kappaB activation in keloid fibroblasts ... In this study, we demonstrate that TNF-alpha induced NF-kappaB activation in keloid fibroblasts, which show more sensitively than the normal skin fibroblasts ... Aspirin pretreatment can inhibit TNF-alpha induced activation of NF-kappaB in a dose dependent manner by preventing the phosphorylation and degradation of IkappaBalpha and nuclear translocation of NF-kappaB ... Aspirin pretreatment can inhibit TNF-alpha induced activation of NF-kappaB in a dose dependent manner by preventing the phosphorylation and degradation of IkappaBalpha and nuclear translocation of NF-kappaB
Chen et al., J Vasc Res 2007 (Vasculitis) : Exposure to Ang II ( 10 ( -6 ) M for 24 h ) also enhanced intracellular ROS elaboration and the levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-8, upregulated chemokine receptor CXCR2 mRNA expression, increased adhesion of endothelial cells to monocytes and induced a significant increase in the activity of nuclear factor (NF)-kappaB , which was attenuated by pretreatment with the Ang II receptor blocker losartan ( 1, 3 and 10 muM ) ... Exposure to Ang II ( 10 ( -6 ) M for 24 h ) also enhanced intracellular ROS elaboration and the levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-8 , upregulated chemokine receptor CXCR2 mRNA expression, increased adhesion of endothelial cells to monocytes and induced a significant increase in the activity of nuclear factor (NF)-kappaB , which was attenuated by pretreatment with the Ang II receptor blocker losartan ( 1, 3 and 10 muM ) ... Exposure to Ang II ( 10 ( -6 ) M for 24 h ) also enhanced intracellular ROS elaboration and the levels of tumor necrosis factor (TNF)-alpha and interleukin (IL)-8, upregulated chemokine receptor CXCR2 mRNA expression, increased adhesion of endothelial cells to monocytes and induced a significant increase in the activity of nuclear factor (NF)-kappaB , which was attenuated by pretreatment with the Ang II receptor blocker losartan ( 1, 3 and 10 muM )
Suzuki et al., Am J Physiol Cell Physiol 2007 (Disease Models, Animal...) : Twenty-four hours thereafter, splenic macrophages were isolated, and their IL-6 and TNF-alpha production and activation of MAPK and NF-kappaB were measured
Nadella et al., Leukemia 2007 (HTLV-I Infections...) : In summary, the data demonstrated that transcriptional regulation of PTHrP in ATLL cells can be controlled by NF-kappaB activation and also suggest a Tax independent mechanism of activation of PTHrP in ATLL
Ren et al., J Mol Endocrinol 2007 : Further study demonstrated that IGF-I induced the DNA binding activity of NF-kappaB in association with decreased expression of the NF-kappaB inhibitory protein IkappaBalpha ... Using a specific phosphatidylinositol 3-kinase (PI3K) inhibitor, LY294002, we also found that PI3K was involved in the pathway by which IGF-I activated NF-kappaB and increased FLIP expression
Partridge et al., FASEB J 2007 : We demonstrated using assays of nuclear localization, NF-kappaB subunit phosphorylation, DNA binding, and transcriptional activity that NF-kappaB is activated by TNFalpha in presheared HUVEC
Weersma et al., Scand J Gastroenterol 2007 (Colitis, Ulcerative...) : The interleukin receptor associated kinase-M ( IRAK-M ) is a NF-kappaB mediated , negative regulator of Toll-like receptor ( TLR ) signaling
Lu et al., Mol Cell 2007 : XIAP induces NF-kappaB activation via the BIR1/TAB1 interaction and BIR1 dimerization ... In addition to caspase inhibition, X-linked inhibitor of apoptosis (XIAP) induces NF-kappaB and MAP kinase activation during TGF-b and BMP receptor signaling and upon overexpression ... Structure based mutagenesis and knockdown of TAB1 show unambiguously that the BIR1/TAB1 interaction is crucial for XIAP induced TAK1 and NF-kappaB activation ... Disruption of BIR1 dimerization abolishes XIAP mediated NF-kappaB activation, implicating a proximity induced mechanism for TAK1 activation
Yamagishi et al., Microvasc Res 2008 (Diabetic Retinopathy...) : Since the crosstalk between the AGEs-RAGE and the renin-angiotensin system has also been proposed in the pathogenesis of PDR, we investigated here whether olmesartan, an angiotensin II type 1 receptor blocker, inhibited the AGEs elicited angiogenesis in vitro by suppressing the NF-kappaB mediated RAGE expression
Chung et al., J Biochem Mol Biol 2007 : However, the detailed mechanism of STP-C mediated NF-kappaB activation has not been reported yet ... NF-kappaB activation by STP-C through the ubiquitinated TRAF6 causes the increased production of IL-8 , an inflammatory chemokine and the enhanced expression of costimulatory molecule ICAM, which might ultimately contribute cellular transformation by the exposure of HVS infected cells with inflammatory microenvironment and chronic activation ... NF-kappaB activation by STP-C through the ubiquitinated TRAF6 causes the increased production of IL-8, an inflammatory chemokine and the enhanced expression of costimulatory molecule ICAM, which might ultimately contribute cellular transformation by the exposure of HVS infected cells with inflammatory microenvironment and chronic activation ... NF-kappaB activation by STP-C through the ubiquitinated TRAF6 causes the increased production of IL-8, an inflammatory chemokine and the enhanced expression of costimulatory molecule ICAM, which might ultimately contribute cellular transformation by the exposure of HVS infected cells with inflammatory microenvironment and chronic activation
Julien et al., Oncogene 2007 (Carcinoma, Squamous Cell...) : Activation of NF-kappaB by Akt upregulates Snail expression and induces epithelium mesenchyme transition ... Here, we show that Snail activation and consequent repression of E-cadherin may depend on AKT mediated nuclear factor-kappaB (NF-kappaB) activation, and that NF-kappaB induces Snail expression ... Here, we show that Snail activation and consequent repression of E-cadherin may depend on AKT mediated nuclear factor-kappaB (NF-kappaB) activation , and that NF-kappaB induces Snail expression
Yang et al., J Cereb Blood Flow Metab 2008 (Ischemic Attack, Transient) : Sumoylation of transcription factors has been shown to block their activation, with some exceptions such as the heat-shock factor and the hypoxia-responsive factor, where sumoylation blocks their degradation, and the nuclear factor-kappaB (NF-kappaB) essential modulator where sumoylation leads to an activation of NF-kappaB
Koike et al., J Gastroenterol Hepatol 2007 (Stomach Neoplasms) : TNF-alpha activated NF-kappaB and up-regulated endogenous TFF1 mRNA expression as well as the transcription of the TFF1 reporter genes in a dose dependent manner
Zhou et al., Virology journal 2007 : These results suggest that E1Blarge interacts with NF-kappaB to prevent transcription and down regulate proinflammatory cytokine IL-8 production
Kumar et al., Molecular cancer 2007 (Brain Neoplasms...) : Our data using IkappaBalpha mutant LN-18 cells and PI3K/Akt inhibitor-LY294002 revealed that the expression of p21 is regulated by NF-kappaB ... Our findings provide evidence that TNF-alpha induced p21 might be regulated by NF-kappaB or p53 independently
Seki et al., J Hepatol 2007 : These results reveal that NEMO mediated NF-kappaB activation in hepatocytes has an essential physiological function to prevent the spontaneous development of steatohepatitis and hepatocellular carcinoma, identifying NEMO as a tumor suppressor in the liver
Hatano et al., J Gastroenterol Hepatol 2007 (Liver Diseases) : The TNF-alpha and Jo2 induced iNOS through NF-kappaB ... The TNF-alpha and Jo2 induced iNOS through NF-kappaB
Iimuro et al., J Gastroenterol Hepatol 2007 : In Myd88 ( -/- ) mice after PH, induction of expression of immediate early genes involved in hepatocyte replication and phosphorylation of signal transducer and activators of transcription 3 ( STAT3 ) in the liver, and production of TNF-alpha/IL-6 by and activation of NF-kappaB in the Kupffer cells were grossly subnormal and were associated with impaired liver regeneration, while TLR2, 4 and 9, which recognize Gram negative and -positive bacterial products, are not essential for NF-kappaB activation and IL-6 production after PH ... In Myd88 ( -/- ) mice after PH, induction of expression of immediate early genes involved in hepatocyte replication and phosphorylation of signal transducer and activators of transcription 3 ( STAT3 ) in the liver, and production of TNF-alpha/IL-6 by and activation of NF-kappaB in the Kupffer cells were grossly subnormal and were associated with impaired liver regeneration, while TLR2, 4 and 9, which recognize Gram negative and -positive bacterial products, are not essential for NF-kappaB activation and IL-6 production after PH ... In Myd88 ( -/- ) mice after PH, induction of expression of immediate early genes involved in hepatocyte replication and phosphorylation of signal transducer and activators of transcription 3 ( STAT3 ) in the liver, and production of TNF-alpha/IL-6 by and activation of NF-kappaB in the Kupffer cells were grossly subnormal and were associated with impaired liver regeneration, while TLR2 , 4 and 9, which recognize Gram negative and -positive bacterial products, are not essential for NF-kappaB activation and IL-6 production after PH
Hong et al., BMC molecular biology 2007 : Zfra affects TNF mediated cell death by interacting with death domain protein TRADD and negatively regulates the activation of NF-kappaB , JNK1, p53 and WOX1 during stress response ... Functionally, transiently overexpressed Zfra sequestered NF-kappaB ( p65 ), WOX1, p53 and phospho-ERK ( extracellular signal activated kinase ) in the cytoplasm, and TNF or UV light could not effectively induce nuclear translocation of these proteins
Xiong et al., Dev Comp Immunol 2008 : When transfected into NIH3T3 cells, Pf-IKK activated the expression of NF-kappaB controlled reporter gene and induced NF-kappaB translocation, whereas the activation was greatly deduced by pyrrolidine dithiocarbamate ( PDTC )
Geisler et al., Gastroenterology 2007 (Drug-Induced Liver Injury) : RelA/p65 is essential for TNF induced NF-kappaB activation in adult hepatocytes ... RelA/p65 is essential for TNF induced NF-kappaB activation in adult hepatocytes ... RelA/p65 is essential for TNF induced NF-kappaB activation in adult hepatocytes
Yu et al., Int Immunopharmacol 2007 (Sepsis) : Ketamine 2.5, 5 and 10 mg/kg after CLP decreased intestinal TNF-alpha level and NF-kappaB activity, and inhibited TLR2 and TLR4 expressions as well ... Ketamine 2.5, 5 and 10 mg/kg after CLP decreased intestinal TNF-alpha level and NF-kappaB activity, and inhibited TLR2 and TLR4 expressions as well
Moon et al., Int Immunopharmacol 2007 : Moreover, BV and MEL suppressed LPS induced activation of nuclear factor kappa B (NF-kappaB) by blocking degradation of IkappaBalpha and phosphorylation of c-Jun N-terminal kinase (JNK) and Akt, which resulted in inhibition of iNOS expression ... Moreover, BV and MEL suppressed LPS induced activation of nuclear factor kappa B (NF-kappaB) by blocking degradation of IkappaBalpha and phosphorylation of c-Jun N-terminal kinase (JNK) and Akt, which resulted in inhibition of iNOS expression
Ornstein et al., Nutr Cancer 2007 (Neoplasm Invasiveness...) : Expression of uPA is regulated in part by the oxidant-sensitive transcription factor, NF-kappa B (NF-kappaB) , which is activated by intracellular reactive oxygen intermediates ( ROI )
Salter et al., Am J Physiol Lung Cell Mol Physiol 2007 (Disease Models, Animal...) : FF demonstrated very potent glucocorticoid activity in several key pathways downstream of the glucocorticoid receptor ( GR ) as follows : the transrepression nuclear factor-kappaB (NF-kappaB) pathway, the transactivation glucocorticoid response element pathway, and inhibition of the proinflammatory cytokine tumor necrosis factor-alpha
Li et al., Mol Biol Rep 2008 : Adriamycin induces myocardium apoptosis through activation of nuclear factor kappaB in rat and NF-kappaB activation requires IkappaB alpha degradation
Yang et al., Chin J Integr Med 2007 (Learning Disorders...) : TCA could improve A beta 25-35 induced dysfunction of learning and memory in rats, and its protective mechanism is associated with its actions in decreasing MDA level, increasing Mn-SOD activity and inhibiting the expression of NF-kappa B in cerebral cortex
Usluoglu et al., Eur J Immunol 2007 : Different signal pathways mediate LPS induced expression of IL-12 and involve PI3K, MAPK and the transcription factor NF-kappaB
Polumuri et al., J Immunol 2007 : LPS induced binding of NF-kappaB to the IL-12 p40 promoter NF-kappaB binding site was not affected by FcgammaR ligation at 1 h ; however, by 4 h, NF-kappaB binding was markedly inhibited, confirmed in situ by chromatin immunoprecipitation analysis
Stuhlmeier et al., J Immunol 2007 (Necrosis) : Apis mellifera venom and melittin block neither NF-kappa B-p50-DNA interactions nor the activation of NF-kappa B , instead they activate the transcription of proinflammatory genes and the release of reactive oxygen intermediates ... EMSAs demonstrate that neither BV nor melittin blocked IL-1beta induced NF-kappaB activation ; neither did they affect phosphorylation or degradation of IkappaB
Kanda et al., Clin Exp Allergy 2007 : CCL27 production by epidermal keratinocytes is dependent on nuclear factor-kappaB (NF-kappaB) activity and is enhanced in lesions with atopic dermatitis or allergic contact dermatitis ... LTB ( 4 ) alone did not enhance CCL27 production and modestly enhanced NF-kappaB activity in human keratinocytes ... However, LTB ( 4 ) potently enhanced TNF-alpha induced CCL27 secretion and mRNA expression and NF-kappaB activity ... However, LTB ( 4 ) potently enhanced TNF-alpha induced CCL27 secretion and mRNA expression and NF-kappaB activity
Lee et al., American journal of physiology. Renal physiology 2007 : In addition, IL-6 increased the level of nuclear factor-kappaB (NF-kappaB) phosphorylation
Sayed et al., Phytother Res 2007 : Thymoquinone decreases AGE induced NF-kappaB activation in proximal tubular epithelial cells ... A significant reduction of AGE induced NF-kappaB-activation and Il-6 expression was observed
Bai et al., Biochem Biophys Res Commun 2007 (MAP Kinase Signaling System) : Rosiglitazone attenuates NF-kappaB dependent ICAM-1 and TNF-alpha production caused by homocysteine via inhibiting ERK1/2/p38MAPK activation ... Rosiglitazone attenuates NF-kappaB dependent ICAM-1 and TNF-alpha production caused by homocysteine via inhibiting ERK1/2/p38MAPK activation
Li et al., Exp Cell Res 2007 : Inhibition of NFkappaB activity prevented stress induced down-regulation of CTCF and increased cell viability against stress induced apoptosis
Trivedi et al., Atherosclerosis 2007 (Inflammation) : Trichostatin A (TSA) rescues HOXA9 mediated suppression of NF-kappaB activity, but HOXA9 interaction with p300 is not responsible for inhibition of EC activation
Carluccio et al., Am J Physiol Heart Circ Physiol 2007 (Hyperhomocysteinemia) : Homocysteine induces VCAM-1 gene expression through NF-kappaB and NAD ( P ) H oxidase activation : protective role of Mediterranean diet polyphenolic antioxidants ... Homocysteine induces VCAM-1 gene expression through NF-kappaB and NAD ( P ) H oxidase activation : protective role of Mediterranean diet polyphenolic antioxidants ... Because VCAM-1 expression is crucial in monocyte adhesion and early atherogenesis, we evaluated the NF-kappaB related induction of VCAM-1 by homocysteine ( Hcy ) and the possible inhibitory effect of dietary polyphenolic antioxidants, such as trans-resveratrol ( RSV ) and hydroxytyrosol ( HT ), which are known inhibitors of NF-kappaB mediated VCAM-1 induction
Fuentes et al., Dis Colon Rectum 2007 (Adenocarcinoma...) : These data indicate that S100P is expressed at greater levels in colon cancer than matched normal tissue and that S100P stimulates colon cancer cell growth, migration, Erk phosphorylation, and NFkappaB activation in vitro, suggesting that this ligand/receptor pair may be targeted for the development of new therapies
Gruber et al., Mol Immunol 2008 : Importantly, however, both atypical PKCzeta and PKCiota were functionally required in TCR/CD28 mediated activation of NF-kappaB downstream of PKCtheta in Jurkat T cells albeit, activation responses of PKCzeta-deficient CD3+ T cells were comparable with wildtype controls
Lee et al., J Nutr Biochem 2008 (Carcinoma, Hepatocellular...) : In nuclear factor-kappa B (NF-kappaB) mediated apoptosis pathway, the results showed that SNL glycoprotein dose-dependently blocked DNA binding activity of NF-kappaB , activity of inducible nitric oxide synthase (iNOS) and production of inducible nitric oxide ( NO )
Szabo et al., Pancreatology 2007 (Pancreatitis, Alcoholic) : The effects of acute or chronic alcohol exposure were evaluated in human monocytes on the production of TNFalpha or IL-10 production, pro-inflammatory gene and nuclear factor-kappaB (NF-kappaB) activation ... The effects of acute or chronic alcohol exposure were evaluated in human monocytes on the production of TNFalpha or IL-10 production, pro-inflammatory gene and nuclear factor-kappaB (NF-kappaB) activation ... Moderate, acute alcohol consumption or equivalent doses of alcohol in vitro had anti-inflammatory effects on monocyte activation via inhibition of pro-inflammatory genes and NF-kappaB activation, inhibition of TNFalpha production and augmentation of the anti-inflammatory cytokine, IL-10 ... In contrast, acute alcohol treatment augmented NF-kappaB activation and TNFalpha production and inhibited IL-10 levels in the presence of complex stimulation with combined TLR2 and TLR4 ligands ... Prolonged alcohol exposure also resulted in an increase in NF-kappaB and TNFalpha production in response to TLR4 stimulation with LPS ... Prolonged alcohol exposure also resulted in an increase in NF-kappaB and TNFalpha production in response to TLR4 stimulation with LPS
Choudhary et al., Mol Endocrinol 2007 : Finally, we demonstrate that Ang II infusion in vivo rapidly induces phospho-Ser ( 536 ) RelA formation and activation of the NF-kappaB dependent IL-6 gene
Roh et al., Cell Physiol Biochem 2007 : Pokemon 1 plays an important role in adipogenesis, osteogenesis, oncogenesis, and transcription of NF-kB responsive genes
Matta et al., J Biol Chem 2007 : The K13-TRAF2 interaction is believed to be essential for the recruitment of K13 to the I-kappaB kinase (IKK) complex and for K13 induced NF-kappaB and JNK activation ... In addition, TRAF3 has been reported to be required for K13 induced NF-kappaB and JNK activation ... Furthermore, endogenously expressed TRAF2 and TRAF3 do not interact with K13 and play no role in K13 induced NF-kappaB activation or its interaction with the IKK complex ... Furthermore, endogenously expressed TRAF2 and TRAF3 do not interact with K13 and play no role in K13 induced NF-kappaB activation or its interaction with the IKK complex ... Furthermore, endogenously expressed TRAF2 and TRAF3 do not interact with K13 and play no role in K13 induced NF-kappaB activation or its interaction with the IKK complex ... Selective NF-kappaB activation by K13 might represent a novel strategy employed by the virus to promote latency
Hashiramoto et al., Arthritis Rheum 2007 (Arthritis, Rheumatoid) : In addition, Ang-1 activated NF-kappaB via Akt to promote cell growth, but also inhibited cell apoptosis via ERK and Akt
Maeda et al., Biochem Biophys Res Commun 2007 (Colitis...) : In macrophages, HMGB1 induces several proinflammatory cytokines, such as IL-6, which are regulated by NF-kappaB activation ... In macrophages, HMGB1 induces several proinflammatory cytokines, such as IL-6, which are regulated by NF-kappaB activation ... LPS induced a small amount of HMGB1 in macrophages, but macrophages incubated with supernatant prepared from necrotic cells and containing large amounts of HMGB1 activated NF-kappaB and induced IL-6 ... LPS induced a small amount of HMGB1 in macrophages, but macrophages incubated with supernatant prepared from necrotic cells and containing large amounts of HMGB1 activated NF-kappaB and induced IL-6
Bae et al., Mol Immunol 2008 (Inflammation) : Reverse signaling initiated from GITRL induces NF-kappaB activation through ERK in the inflammatory activation of macrophages ... Immunofluorescence analysis detected nuclear translocation of the NF-kappaB p50 subunit and this was blocked by ERK inhibitor, indicating that GITRL stimulation induced ERK1/2 phosphorylation and subsequent activation of NF-kappaB
Kralisch et al., Atherosclerosis 2008 : Taken together, we demonstrate that endothelial dysfunction is induced by adipocyte secreted factors via NFkappaB partly dependent on TNFalpha
Fan et al., Life Sci 2007 (Cardiomyopathies...) : In the present study, CFs obtained from Sprague-Dawley rats were stimulated with or without AVP and pyrrolidine dithiocarbamate ( PDTC ), a specific inhibitor of nuclear factor kappa-B (NF-kappaB) ... In addition, it was found that NF-kappaB was activated by AVP, and that PDTC could inhibit NO production, NOS activity, protein and mRNA levels of iNOS stimulated by AVP in a dose dependent manner ... In addition, it was found that NF-kappaB was activated by AVP, and that PDTC could inhibit NO production, NOS activity, protein and mRNA levels of iNOS stimulated by AVP in a dose dependent manner ... In addition, it was found that NF-kappaB was activated by AVP , and that PDTC could inhibit NO production, NOS activity, protein and mRNA levels of iNOS stimulated by AVP in a dose dependent manner
Curry et al., Mol Cancer Ther 2007 (Sarcoma, Kaposi) : Further analysis showed the induction of cyclin B1 was due , at least in part, to increased nuclear factor-kappaB (NF-kappaB) activity, which was also required for the G(2)-M growth arrest after Notch inhibition
Saegusa et al., J Pathol 2007 (Endometrial Neoplasms) : In cell lines, overexpression of p65 inhibited beta-catenin/TCF4 mediated transcription, while transfection of GSK-3beta resulted in repression of TNF-alpha induced NF-kappaB activity ... In cell lines, overexpression of p65 inhibited beta-catenin/TCF4 mediated transcription, while transfection of GSK-3beta resulted in repression of TNF-alpha induced NF-kappaB activity
Pingle et al., Mol Pharmacol 2007 : Furthermore, A ( 1 ) AR agonists inhibited iNOS expression in a nuclear factor-kappaB (NF-kappaB) dependent manner
Ramakers et al., Lipids 2007 : Furthermore, ARA increased NF-kappaB activation in a reporter cell-line as compared to EPA ... Our results showed that ARA but not EPA and OA activates NF-kappaB and elevates ICAM-1 expression in Caco-2 enterocytes
Karrasch et al., PloS one 2007 (Colitis) : Caspase 3 activation was significantly enhanced whereas COX-2 gene expression was reduced in luteolin fed, DSS exposed NF-kappaB ( EGFP ) transgenic mice as assessed by Western blot and immunohistochemical analysis
Simiantonaki et al., Int J Oncol 2007 (Adenocarcinoma...) : Considering the fact that cancer cell necrosis plays a crucial role in metastasis, NFkappaB activation mediated by loss of E-cadherin may represent an essential, even initial event in this process
Jeong et al., Int J Neurosci 2007 : YHT also inhibited LPS induced activation of NF-kappaB and degradation of IkappaBalpha
Hall et al., Cancer Cell 2007 (Neoplasms) : However, TRAIL signaling also activates NF-kappaB , which induces the antiapoptotic regulators Mcl-1 and cIAP2, thus compromising its efficacy ... In this issue of Cancer Cell, El-Deiry and colleagues explore pathways that disrupt TRAIL induced survival signaling and show that the Myc oncoprotein and the Raf kinase inhibitor Sorafenib sensitize otherwise TRAIL-resistant colon cancer cells by effectively reducing NF-kappaB mediated transcription of Mcl-1
Ricci et al., Cancer Cell 2007 : TRAIL induced expression of antiapoptotic Mcl-1 and cIAP2 through activation of NF-kappaB ... TRAIL induced expression of antiapoptotic Mcl-1 and cIAP2 through activation of NF-kappaB ... TRAIL induced expression of antiapoptotic Mcl-1 and cIAP2 through activation of NF-kappaB
Polk et al., Am J Physiol Cell Physiol 2007 (Nephritis) : RhoA regulation of NF-kappaB activation is mediated by COX-2 dependent feedback inhibition of IKK in kidney epithelial cells ... RhoA regulation of NF-kappaB activation is mediated by COX-2 dependent feedback inhibition of IKK in kidney epithelial cells ... Conversely, constitutive expression of RhoA prevented NF-kappaB activation by LPS, and this effect was reversed by cotransfection with MEKK1-KD ... Conversely, constitutive expression of RhoA prevented NF-kappaB activation by LPS , and this effect was reversed by cotransfection with MEKK1-KD
Li et al., Molecular vision 2007 : Cultured limbal and conjunctival cells responded to stimulation by polyriboinosinic polyribocytidylic acid ( poly [ I:C ] ), palmitoyl-3-cysteine-serine-lysine-4 ( Pam3CSK ) and flagellin with increased secretion of IL-6 and IL-8 and the activation of NFkappaB ... Cultured limbal and conjunctival cells responded to stimulation by polyriboinosinic polyribocytidylic acid ( poly [ I:C ] ), palmitoyl-3-cysteine-serine-lysine-4 ( Pam3CSK ) and flagellin with increased secretion of IL-6 and IL-8 and the activation of NFkappaB
Chan et al., J Pharmacol Exp Ther 2007 (Acute Disease...) : This study sought to elucidate the mechanism mediating angiotensin II involvement in angiotensin II type 1 (AT1) receptor mediated NF-kappaB activation, and ultimately in proinflammatory actions of AP pathogenesis ... These data provide substantial evidence that angiotensin II is involved in AT1 receptor mediated NADPH oxidase dependent NF-kappaB activation ; thus, it might ultimately promote proinflammatory actions during AP pathogenesis
Laga et al., Biochem Pharmacol 2007 : Methylglyoxal suppresses TNF-alpha induced NF-kappaB activation by inhibiting NF-kappaB DNA binding ... In this study, we show that methylglyoxal inhibits TNF induced NF-kappaB activation and NF-kappaB dependent reporter gene expression by inhibiting the DNA binding capacity of NF-kappaB p65 ... In this study, we show that methylglyoxal inhibits TNF induced NF-kappaB activation and NF-kappaB dependent reporter gene expression by inhibiting the DNA binding capacity of NF-kappaB p65
Albanesi et al., J Immunol 2007 : This interferes with STAT-1 and NF-kappaB signaling, thereby inhibiting TNF-alpha/IFN-gamma mediated induction of HBD-2 and HBD-3 ... This interferes with STAT-1 and NF-kappaB signaling, thereby inhibiting TNF-alpha/IFN-gamma mediated induction of HBD-2 and HBD-3
Kato et al., J Immunol 2007 (Asthma...) : The induction of TSLP by dsRNA was dependent upon NF-kappaB and IFN regulatory factor 3 (IRF-3) signaling via TLR3 as indicated by a study with small interfering RNA
Tang et al., J Immunol 2007 : Leptin induced IL-6 production is mediated by leptin receptor, insulin receptor substrate-1, phosphatidylinositol 3-kinase, Akt, NF-kappaB , and p300 pathway in microglia ... Leptin mediated IL-6 production was attenuated by OBRl receptor antisense oligonucleotide, PI3K inhibitor ( Ly294002 and wortmannin ), Akt inhibitor ( 1L-6-hydroxymethyl-chiro-inositol-2- ( ( R ) -2-O-methyl-3-O-octadecylcarbonate ) ), NF-kappaB inhibitor ( pyrrolidine dithiocarbamate ), IkappaB protease inhibitor ( L-1-tosylamido-2-phenylenylethyl chloromethyl ketone ), IkappaBalpha phosphorylation inhibitor ( Bay 117082 ), or NF-kappaB inhibitor peptide ... The binding of p65 and p50 to the NF-kappaB elements, as well as the recruitment of p300 and the enhancement of histone H3 and H4 acetylation on the IL-6 promoter was enhanced by leptin
Kaplan et al., Shock 2007 : Overexpression of a dominant negative PPARgamma mutant prevented the inhibitory effect of ciglitazone on cytokine induced NFkappaB activation in transfected human endothelial cells
Koide et al., J Endotoxin Res 2007 : LPS significantly augmented the activation of interferon regulatory factor (IRF)-1 in IFN-gamma stimulated END-D cells, although it did not affect the activation of either MyD88 dependent nuclear factor (NF)-kappaB or MyD88 independent IRF-3
Hayakawa et al., J Biol Chem 2007 (Asthma...) : Soluble ST2 directly bound to IL-33 and suppressed activation of NF-kappaB in EL-4 cells stably expressing ST2L, suggesting that the complex of soluble ST2 and IL-33 fails to bind to ST2L
Dekelbab et al., Steroids 2007 : The synthetic glucocorticoid, dexamethasone ( DEX ) blunted TNF-alpha stimulated NF-kappaB activation in L6 cells
Suzuki et al., J Biol Chem 2007 (Cell Transformation, Viral...) : Constitutive activation of TAK1 by HTLV-1 tax dependent overexpression of TAB2 induces activation of JNK-ATF2 but not IKK-NF-kappaB ... Constitutive activation of TAK1 by HTLV-1 tax dependent overexpression of TAB2 induces activation of JNK-ATF2 but not IKK-NF-kappaB ... Surprisingly, TAK1 was not involved in the activation of NF-kappaB
Suh et al., J Virol 2007 (Cytomegalovirus Infections...) : PIC induction of IDO was mediated in part by IFN-beta but not IFN-gamma, and both NF-kappaB and interferon regulatory factor 3 (IRF3) were required ... PIC induction of IDO was mediated in part by IFN-beta but not IFN-gamma, and both NF-kappaB and interferon regulatory factor 3 (IRF3) were required ... PIC induction of IDO was mediated in part by IFN-beta but not IFN-gamma , and both NF-kappaB and interferon regulatory factor 3 (IRF3) were required ... PIC induction of IDO was mediated in part by IFN-beta but not IFN-gamma, and both NF-kappaB and interferon regulatory factor 3 (IRF3) were required
Wati et al., J Virol 2007 (Dengue) : Additionally, normal cellular TNF-alpha signaling, measured by quantitation of TNF-alpha induced stimulation of transcription from an NF-kappaB-responsive reporter plasmid or NF-kappaB protein nuclear translocation, was blocked in DV-infected MDM and Huh7 cells
Albor et al., Mol Carcinog 2007 (Carcinoma, Squamous Cell...) : Piasy inhibits NF-kappaB activity, and promotes keratinocyte apoptosis in response to UV and TNF-alpha
Ogawa et al., Inflamm Res 2007 (Anoxia...) : These results suggest that TLR4 dependent NF-kB activation may be an important process of the upregulation of proinflammatory mediators and subsequent neutrophil accumulation into the lung during hyperoxia
Harimaya et al., Int J Pediatr Otorhinolaryngol 2007 (Gram-Positive Bacterial Infections...) : We investigated the role of NF-kappaB and mitogen activated protein ( MAP ) kinases in chemokine interleukin-8 (IL-8) production by human peripheral blood mononuclear cells ( PBMCs ) after stimulation with A. otitidis ... And, inhibitors of NF-kappaB ( caffeic acid phenylethyl ester ), p38 ( SB 203580 ), or ERK1/2 ( PD 98059 ) significantly reduced IL-8 induction by the organism
Wei et al., Biochem Pharmacol 2007 (Adenocarcinoma...) : Activation of ERK and p38 MAPK appeared to be mediated primarily by transactivation of EGFR, whereas activation of CREB and NF-kappaB was mediated by PKA, PKC and ERK ... Activation of ERK and p38 MAPK appeared to be mediated primarily by transactivation of EGFR, whereas activation of CREB and NF-kappaB was mediated by PKA , PKC and ERK ... The role of CREB and NF-kappaB in I-BOP induced COX-2 expression was further explored at the promoter level
Karlsen et al., J Nutr 2007 : In cultured monocytes, anthocyanins isolated from bilberries and black currants ( Medox ) efficiently suppressed LPS induced activation of NF-kappaB
Nakamura et al., Rheumatol Int 2007 (Sjogren's Syndrome) : EGF activates PI3K-Akt and NF-kappaB via distinct pathways in salivary epithelial cells in Sjögren 's syndrome ... Chemical inhibition of PI3K-Akt by LY294002/wortmannin did not affect EGF mediated NF-kappaB p65 nuclear translocation ; and NF-kappaB inhibition by Bay 11-7082 did not suppress Akt phosphorylation ... Chemical inhibition of PI3K-Akt by LY294002/wortmannin did not affect EGF mediated NF-kappaB p65 nuclear translocation ; and NF-kappaB inhibition by Bay 11-7082 did not suppress Akt phosphorylation ... Chemical inhibition of PI3K-Akt by LY294002/wortmannin did not affect EGF mediated NF-kappaB p65 nuclear translocation ; and NF-kappaB inhibition by Bay 11-7082 did not suppress Akt phosphorylation ... Chemical inhibition of PI3K-Akt by LY294002/wortmannin did not affect EGF mediated NF-kappaB p65 nuclear translocation ; and NF-kappaB inhibition by Bay 11-7082 did not suppress Akt phosphorylation ... Our data suggest that EGF stimulates both the PI3K-Akt pathway and NF-kappaB via distinct mechanisms, promoting tropic effects in SS salivary epithelial cells
Avvisato et al., J Cell Sci 2007 (Colonic Neoplasms) : The shear stress induced increase of the secreted Wnt inhibitor DKK1 requires p38 and activation of NFkappaB requires IkappaB kinase-beta
Vaziri et al., J Pharmacol Exp Ther 2007 (Disease Models, Animal...) : The untreated group exhibited hypertension, deterioration of renal function and structure, reduced or unchanged plasma renin activity, aldosterone concentration, marked up-regulations of AT1r ( 250 % ), Ang II-expressing cell count ( > 20-fold ), NAD ( P ) H oxidase subunits ( gp91(phox,) p22(phox), and P47 ( phox ) ; 20-40 % ), COX-2 ( 250 % ), 12-lipooxygenase ( 100 % ), MCP-1 ( 400 % ), and PAI-1 ( > 20-fold ), activation of NFkappaB , and interstitial infiltrations of T cells and macrophages in the remnant kidneys ... The untreated group exhibited hypertension, deterioration of renal function and structure, reduced or unchanged plasma renin activity, aldosterone concentration, marked up-regulations of AT1r ( 250 % ), Ang II-expressing cell count ( > 20-fold ), NAD ( P ) H oxidase subunits ( gp91(phox,) p22(phox), and P47 ( phox ) ; 20-40 % ), COX-2 ( 250 % ), 12-lipooxygenase ( 100 % ), MCP-1 ( 400 % ), and PAI-1 ( > 20-fold ), activation of NFkappaB , and interstitial infiltrations of T cells and macrophages in the remnant kidneys ... The untreated group exhibited hypertension, deterioration of renal function and structure, reduced or unchanged plasma renin activity, aldosterone concentration, marked up-regulations of AT1r ( 250 % ), Ang II-expressing cell count ( > 20-fold ), NAD ( P ) H oxidase subunits ( gp91(phox,) p22(phox), and P47 ( phox ) ; 20-40 % ), COX-2 ( 250 % ), 12-lipooxygenase ( 100 % ), MCP-1 ( 400 % ), and PAI-1 ( > 20-fold ), activation of NFkappaB , and interstitial infiltrations of T cells and macrophages in the remnant kidneys ... The untreated group exhibited hypertension, deterioration of renal function and structure, reduced or unchanged plasma renin activity, aldosterone concentration, marked up-regulations of AT1r ( 250 % ), Ang II-expressing cell count ( > 20-fold ), NAD ( P ) H oxidase subunits ( gp91(phox,) p22(phox), and P47 ( phox ) ; 20-40 % ), COX-2 ( 250 % ), 12-lipooxygenase ( 100 % ), MCP-1 ( 400 % ), and PAI-1 ( > 20-fold ), activation of NFkappaB , and interstitial infiltrations of T cells and macrophages in the remnant kidneys ... The untreated group exhibited hypertension, deterioration of renal function and structure, reduced or unchanged plasma renin activity, aldosterone concentration, marked up-regulations of AT1r ( 250 % ), Ang II-expressing cell count ( > 20-fold ), NAD ( P ) H oxidase subunits ( gp91(phox,) p22(phox), and P47 ( phox ) ; 20-40 % ), COX-2 ( 250 % ), 12-lipooxygenase ( 100 % ), MCP-1 ( 400 % ), and PAI-1 ( > 20-fold ), activation of NFkappaB , and interstitial infiltrations of T cells and macrophages in the remnant kidneys ... The untreated group exhibited hypertension, deterioration of renal function and structure, reduced or unchanged plasma renin activity, aldosterone concentration, marked up-regulations of AT1r ( 250 % ), Ang II-expressing cell count ( > 20-fold ), NAD ( P ) H oxidase subunits ( gp91(phox,) p22(phox), and P47 ( phox ) ; 20-40 % ), COX-2 ( 250 % ), 12-lipooxygenase ( 100 % ), MCP-1 ( 400 % ), and PAI-1 ( > 20-fold ), activation of NFkappaB , and interstitial infiltrations of T cells and macrophages in the remnant kidneys
Yamini et al., Cancer Res 2007 : Temozolomide inhibits basal and tumor necrosis factor alpha (TNFalpha) induced NF-kappaB transcriptional activity without altering phosphorylation or degradation of inhibitor of kappaB-alpha
Lerner-Marmarosh et al., FASEB J 2007 : Presently, we show that hBVR increases PKC-zeta autophosphorylation, stimulation by TNF-alpha , as well as cytokine stimulation of NF-kappaB DNA binding and promoter activity
Ding et al., Cancer Sci 2007 : The IKKbeta/NF-kappaB dependent COX-2 induction was further confirmed in mouse embryonic fibroblasts with IKKbeta deficiency ( IKKbeta ( -/- ) ) and in those that expressed reconstituted IKKbeta
Sandur et al., Free Radic Biol Med 2007 : We found that TNF mediated NF-kappaB activation was inhibited by curcumin ; and glutathione reversed the inhibition
Chew et al., J Biol Chem 2007 : The overexpression of LPXN in mouse A20 B lymphoma cells led to the suppression of BCR induced activation of JNK, p38 MAPK, and, to a lesser extent, Akt, but not ERK and NFkappaB , suggesting that LPXN can selectively repress BCR signaling ... The overexpression of LPXN in mouse A20 B lymphoma cells led to the suppression of BCR induced activation of JNK, p38 MAPK, and, to a lesser extent, Akt, but not ERK and NFkappaB , suggesting that LPXN can selectively repress BCR signaling
Touma et al., J Immunol 2007 : The effect of I kappa BNS on the IL-2 promoter, unlike c-Rel, is dependent on the NF-kappaB rather than the CD28RE site ; mutation of the NF-kappaB site extinguishes the induction of transcription by I kappa BNS in transfectants and prevents association of I kappa BNS with IL-2 promoter DNA
Sethi et al., J Immunol 2007 : Indeed we found that TNF induced NF-kappaB activation was abrogated in MKK4 gene deleted cells, as determined by DNA binding ... NF-kappaB reporter assay revealed that NF-kappaB activation induced by TNF , TNFR1, TRADD, TRAF2, NIK, and I kappaB alpha kinase was modulated in gene deleted cells ... NF-kappaB reporter assay revealed that NF-kappaB activation induced by TNF, TNFR1 , TRADD, TRAF2, NIK, and I kappaB alpha kinase was modulated in gene deleted cells ... NF-kappaB reporter assay revealed that NF-kappaB activation induced by TNF, TNFR1, TRADD , TRAF2, NIK, and I kappaB alpha kinase was modulated in gene deleted cells ... NF-kappaB reporter assay revealed that NF-kappaB activation induced by TNF, TNFR1, TRADD, TRAF2 , NIK, and I kappaB alpha kinase was modulated in gene deleted cells ... NF-kappaB reporter assay revealed that NF-kappaB activation induced by TNF, TNFR1, TRADD, TRAF2, NIK, and I kappaB alpha kinase was modulated in gene deleted cells ... NF-kappaB reporter assay revealed that NF-kappaB activation induced by TNF, TNFR1, TRADD, TRAF2, NIK, and I kappaB alpha kinase was modulated in gene deleted cells
Schweighofer et al., Clin Hemorheol Microcirc 2007 : DSCR1 is induced by NFAT or NF-kappaB and limits Ca ( ++ ) signaling in a negative feed-back loop
Amigó et al., Br J Pharmacol 2007 (Disease Models, Animal...) : Antipsoriatic effects of avarol-3'-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin
Redlak et al., J Surg Res 2008 (Stomach Neoplasms) : The aim of this study was to investigate the effect of DC and ASA on NF-kappaB signaling, and determine its role in programmed cell death in a human gastric carcinoma cell line ... Although, ASA itself had no effect on the NF-kappaB pathway, nor did it reduce DC-induced NF-kappaB translocation, it did prevent DC-induced caspase-3, -6 and -9 activation, poly ( ADP-ribose ) polymerase and lamin A processing, DNA degradation, and PKC signaling, all indices of apoptosis
Feng et al., Cell Signal 2007 : In addition, full-length RIP3 induces both caspase dependent and independent apoptosis, as well as activates NF-kappaB
Yamaguchi et al., Cell Signal 2007 : Importantly, our data suggest that, upon exposure to oxidative stress, PKCdelta mediated IKKalpha activation does not contribute to NF-kappaB activation ; instead, nuclear IKKalpha regulates the transcription activity of the p53 tumor suppressor by phosphorylation at Ser20
Zhou et al., Cytokine 2007 (MAP Kinase Signaling System...) : IL-17F was less potent but induced a significant activation of p65 NFkappaB
Chandrasekaran et al., J Mol Graph Model 2008 : Oxidative stimuli in the cytosol are associated with nuclear translocation of NF-kappa B, whereas in the nucleus, reductive activation by thioredoxin is required for NF-kappa B to bind to DNA and activate target genes
Salanova et al., J Biol Chem 2007 : We used specific antibodies in granulocyte macrophage colony stimulating factor treated neutrophils and observed that acquired GPIIb/IIIa receptors co-localized with beta2-integrins and cooperated in NF-kappaB activation ... We show that Src and Syk non-receptor tyrosine kinases, as well as the actin cytoskeleton, control NF-kappaB activation ... The compounds also inhibited NF-kappaB dependent tumor necrosis factor-alpha mRNA up-regulation
Jang et al., FEMS Immunol Med Microbiol 2007 : However, although Dex did not inhibit the nuclear translocation of p65 NF-kappaB in response to IL-1beta , it profoundly inhibited NF-kappaB promoter- and HBD-2 promoter-driven luciferase activities
Kim et al., J Med Food 2007 (Astrocytoma) : Further studies revealed that resveratrol suppressed LPS induced nuclear translocation and activation of nuclear factor kappaB (NF-kappaB)
Samal et al., Peptides 2007 : PACAP in both developmental and acute regulatory paradigms acts on target genes also regulated by either TNFalpha or TGFbeta, two first messengers acting on transcription mainly through NFkappaB and Smads, respectively
Peng et al., Toxicology 2007 (Glioma) : Inhibition of ERK, p38 and JNK block the activation of AP-1 and NF-kappaB , suggesting these MAPKs are involved in ( Ac ) ( 5 ) GP-induced transcription regulation ... Inhibition of ERK , p38 and JNK block the activation of AP-1 and NF-kappaB , suggesting these MAPKs are involved in ( Ac ) ( 5 ) GP-induced transcription regulation
Kim et al., Nitric Oxide 2007 : Further, quercetin inhibited zymosan induced nuclear factor (NF)-kappaB transcriptional activity but also NF-kappaB dependent iNOS promoter activity
Patel et al., J Biol Chem 2007 : Interleukin-17 stimulates C-reactive protein expression in hepatocytes and smooth muscle cells via p38 MAPK and ERK1/2 dependent NF-kappaB and C/EBPbeta activation ... IL-17 induced CRP promoter-driven reporter gene activity that could be attenuated by dominant negative IkappaBalpha or C/EBPbeta knockdown and stimulated both NF-kappaB and C/EBP DNA binding and reporter gene activities ... These results, confirmed in primary human hepatocytes and CASMC, demonstrate for the first time that IL-17 is a potent inducer of CRP expression via p38 MAPK and ERK1/2 dependent NF-kappaB and C/EBPbeta activation and suggest that IL-17 may mediate chronic inflammation, atherosclerosis, and thrombosis ... These results, confirmed in primary human hepatocytes and CASMC, demonstrate for the first time that IL-17 is a potent inducer of CRP expression via p38 MAPK and ERK1/2 dependent NF-kappaB and C/EBPbeta activation and suggest that IL-17 may mediate chronic inflammation, atherosclerosis, and thrombosis
Lefort et al., J Virol 2007 : Of interest, ifn-beta gene activation by K-bZIP is independent of interferon ( IFN ) -responsive factor 3 ( IRF-3 ) and nuclear factor kappaB (NF-kappaB) activation
Takada et al., Ann N Y Acad Sci 2007 : One signaling is a TAK1/TAB1/NIK cascade activated by TNF-alpha and IL-1, and the activated NF-kappaB blocked the DNA binding of PPAR-gamma , attenuating the activated PPAR mediated adipogenesis
Cirillo et al., J Vasc Res 2007 (Atherosclerosis) : Superoxide dimutase, Rho A ( Y-27639 ) and NF-kappaB inhibitors ( pyrrolidine dithiocarbamate ammonium, Bay 11-7082 ) suppressed nicotine effects on CAM expression
Lee et al., J Biomed Sci 2008 (Colitis...) : In addition, consumption of UDN glycoprotein attenuated the activities of proliferating cell nuclear antigen ( PCNA ), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) , and inhibited the DNA binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) in the mice colonic tissue ... In addition, consumption of UDN glycoprotein attenuated the activities of proliferating cell nuclear antigen ( PCNA ), inducible nitric oxide synthase (iNOS) , and cyclooxygenase-2 (COX-2), and inhibited the DNA binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) in the mice colonic tissue ... In addition, consumption of UDN glycoprotein attenuated the activities of proliferating cell nuclear antigen ( PCNA ), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), and inhibited the DNA binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) in the mice colonic tissue
Reidy et al., Biosci Rep 2007 (Carcinoma, Hepatocellular...) : Apoptosis of dedifferentiated hepatoma cells is independent of NF-kappaB activation in response to LPS
Pekar et al., Reproduction 2007 (Abnormalities, Multiple...) : p53 regulates cyclophosphamide teratogenesis by controlling caspases 3, 8, 9 activation and NF-kappaB DNA binding
Lundberg et al., Blood 2007 (Inflammation) : Furthermore, TLR3 stimulation did not activate NFkappaB , MAPKs, or IRF-3 in DCs and MOs, but was able to do so in ECs and RA-SF
Rajesh et al., Am J Physiol Heart Circ Physiol 2007 (Disease Models, Animal...) : TNF-alpha induced NF-kappaB and RhoA activation and upregulation of adhesion molecules ICAM-1 and VCAM-1, increased expression of monocyte chemoattractant protein, enhanced transendothelial migration of monocytes, and augmented monocyte-endothelial adhesion
Lam et al., J Biol Chem 2007 : Blocking the activity of Akt demonstrated a crucial role for Akt in translocation of STAT-1alpha and NF-kappaB to the nucleus and activation of the CD40 promoter
Zhou et al., Eur J Pharmacol 2007 (Edema) : To investigate the mechanism involved, we examined the effect of 21alpha-MMD and 21beta-MMD on LPS induced nuclear factor-kappaB (NF-kappaB) activation ... To investigate the mechanism involved, we examined the effect of 21alpha-MMD and 21beta-MMD on LPS induced nuclear factor-kappaB (NF-kappaB) activation ... Both 21alpha-MMD and 21beta-MMD significantly inhibited LPS induced NF-kappaB transcriptional activity in RAW 264.7 macrophages ... Both 21alpha-MMD and 21beta-MMD significantly inhibited LPS induced NF-kappaB transcriptional activity in RAW 264.7 macrophages
Lafarge et al., BMC molecular biology 2007 : In response to IL1beta, or IL10 , the levels of phosphorylated NF-kappaB and STAT3 -- respectively -- increased significantly for all the studied cell types ... In response to IL1beta , or IL10, the levels of phosphorylated NF-kappaB and STAT3 -- respectively -- increased significantly for all the studied cell types
Barton et al., Eur Respir J 2007 : Nod1 has been shown to activate nuclear factor (NF)-kappaB ... Activation of Nod1 in these cells resulted in modest activation of NF-kappaB , together with strong activation of the C/EBP transcription factors, particularly C/EBPbeta
Aghai et al., Pediatr Res 2007 (Bronchopulmonary Dysplasia) : Stimulation of TA cells by TNF-alpha increased the activation of NF-kappaB , which was suppressed by the addition of AZM
Anderson et al., Nucleic Acids Res 1991 (Plasmacytoma) : J558L differs from S107, another myeloma which was found to be deficient in the synthesis of NF-kappa B but not in the activation of TIS11
Tamassia et al., Eur J Immunol 2007 : The IFN-gamma induced STAT1 activation and the lipopolysaccharide (LPS) induced NF-kappaB activation were not enhanced if neutrophils were exposed to both stimuli, whereas both transcription factors were activated by IFN-gamma or LPS in monocytes ... The IFN-gamma induced STAT1 activation and the lipopolysaccharide (LPS) induced NF-kappaB activation were not enhanced if neutrophils were exposed to both stimuli, whereas both transcription factors were activated by IFN-gamma or LPS in monocytes
Kim et al., Biochem Biophys Res Commun 2007 : Artemisolide is a typical inhibitor of IkappaB kinase beta targeting cysteine-179 residue and down-regulates NF-kappaB dependent TNF-alpha expression in LPS activated macrophages ... Here, we demonstrate that ATM is a typical inhibitor of IkappaB kinase beta (IKKbeta), resulting in inhibition of lipopolysaccharide (LPS) induced NF-kappaB activation in RAW 264.7 macrophages ... Further, we demonstrate that ATM down-regulates NF-kappaB dependent TNF-alpha expression
Lee et al., Cardiovasc Res 2007 : Down-regulation of NQO1 inhibits MMP-9 gene expression by suppressing activation of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1), well-known key elements mediating MMP-9 gene expression in its promoter, via the p38 MAPK and JNK pathways ... Down-regulation of NQO1 inhibits MMP-9 gene expression by suppressing activation of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1), well-known key elements mediating MMP-9 gene expression in its promoter, via the p38 MAPK and JNK pathways
Tung et al., Cell Signal 2007 : In this study, we identified a requirement for PDGFR, PI3-K/Akt, p38 MAPK, JNK, and NF-kappaB in the regulation of VCAM-1 expression by rat vascular smooth muscle cells ( VSMCs ) in response to viral infection ... Furthermore, VCAM-1 expression induced by EV71 was significantly attenuated by a selective NF-kappaB inhibitor ( helenalin )
Lin et al., Mol Pharmacol 2007 : PPAR-alpha overexpression also inhibited the doxorubicin induced activity of nuclear factor-kappaB (NF-kappaB) , which was associated with the interaction between PPAR-alpha and NF-kappaB p65 subunit as revealed in immunoprecipitation assays
Yan et al., Autophagy 2007 (Cell Transformation, Viral) : We recently found that specific inhibition of Hsp90 by geldanamycin ( GA ) results in autophagic degradation of IKK and NFkappaB inducing kinase (NIK) , an upstream kinase of IKK, and inactivation of NFkappaB in various cell lines ... Here, we further report that GA inhibition of Hsp90 also led to IKK autophagic degradation and NFkappaB inhibition in both HTLV transformed T cells and ATL derived cell lines ... Here, we further report that GA inhibition of Hsp90 also led to IKK autophagic degradation and NFkappaB inhibition in both HTLV transformed T cells and ATL derived cell lines
Lee et al., Oncol Rep 2007 (Urinary Bladder Neoplasms) : Aqueous extract of Magnolia officinalis mediates proliferative capacity, p21WAF1 expression and TNF-alpha induced NF-kappaB activity in human urinary bladder cancer 5637 cells ; involvement of p38 MAP kinase ... Furthermore, the transactivation of TNF-alpha stimulated NF-kappaB was inhibited by SB203580 treatment
Dias et al., Cell Microbiol 2008 : In this work we demonstrate that binding of a recombinant inactive trans-sialidase to molecules containing alpha2,3 linked sialic acid on endothelial cell surface triggers NF-kappaB activation, expression of adhesion molecules and upregulation of parasite entry into host cells
Tsai et al., Int Immunopharmacol 2007 : YC-1, in a cGMP independent manner, inhibited lipopolysaccharide induced Akt activation, IkappaBalpha degradation and NF-kappaB translocation, all of which are associated with co-stimulatory molecules expression
Li et al., Int Immunopharmacol 2007 (Melanoma) : Virulizin also induced the phosphorylation of IkappaB, suggesting that induction of TNFalpha expression by Virulizin is mediated by activation of NFkappaB
Pandey et al., Blood 2007 (Neoplasm Metastasis) : GA suppressed NF-kappaB activation induced by various inflammatory agents and carcinogens and this, accompanied by the inhibition of TAK1/TAB1 mediated IKK activation, inhibited IkappaBalpha phosphorylation and degradation, suppressed p65 phosphorylation and nuclear translocation, and finally abrogated NF-kappaB dependent reporter gene expression
Wang et al., J Biol Chem 2007 (Thrombosis) : A chromatin immunoprecipitation assay and a promoter-luciferase reporter system confirm that TSA inhibits TF-kappaB but not NF-kappaB activation
Lee et al., Biochem Pharmacol 2007 (Colitis...) : TMMC inhibited TNF-alpha induced nuclear factor kappaB (NF-kappaB) activation directly and indirectly
Ghosh et al., Biochem J 2007 : We have demonstrated using co-transfection assays that Sirt1 and TLE1 repress NF-kappaB activity ... We have demonstrated using co-transfection assays that Sirt1 and TLE1 repress NF-kappaB activity ... The catalytic mutant of Sirt1, Sirt1-H363Y, and the N-terminal Sirt1 fragment ( amino acids 1-270 ) also show similar repression activity, suggesting that the deacetylase activity of Sirt1 may not be critical for its effect on NF-kappaB activity ... Furthermore, analysis in Sirt1-null MEFs ( murine embryonic fibroblasts ) and HeLa cells stably expressing siRNA ( small interfering RNA ) specific to Sirt1 or TLE1 demonstrate that both Sirt1 and TLE1 are required for negative regulation of NF-kappaB activity ... Furthermore, analysis in Sirt1-null MEFs ( murine embryonic fibroblasts ) and HeLa cells stably expressing siRNA ( small interfering RNA ) specific to Sirt1 or TLE1 demonstrate that both Sirt1 and TLE1 are required for negative regulation of NF-kappaB activity
Zhao et al., Exp Hematol 2007 (Lymphoma, B-Cell) : In addition, SAHA induced IkappaBalpha cleavage to a stable inhibitory form and caused NF-kappaB degradation in response to caspase-3 activation
Gibbs et al., Int J Cancer 2007 (Breast Neoplasms) : Overexpression of hBVR enhanced both the basal and TNF-alpha mediated activation of NF-kappaB and also that of the NF-kappaB activated iNOS gene
Ma et al., J Gastroenterol Hepatol 2008 (Disease Models, Animal...) : Con A-induced iNOS expression in the liver is dependent on the activation of NF-kappaB
Yoon et al., Osteoarthritis Cartilage 2008 (Chondrosarcoma) : TNF-alpha induced MMP-13 expression was not suppressed by extracellular signal regulated kinase ( ERK ) inhibitors ( PD98059 and U0126 ) or a c-jun terminal kinase ( JNK ) inhibitor ( SP600125 ), but was inhibited by inhibitors of p38 kinase ( SB203580 ) and NF-kappaB ( SN-50 peptide ) and dnIKKalpha and dnIKKbeta
Lindén et al., Science's STKE : signal transduction knowledge environment 2007 : Act1 mediates activation of NF-kappaB and the subsequent production of IL-6 and chemokines that are chemotactic for neutrophils and macrophages
Rico et al., BMC genomics 2007 (Inflammation) : Two not previously described distal regions in rodents that are similar to the unique upstream region responsible of the NF-kappaB activation of NOS-2 in humans are fragmented and translocated to different locations in the rodent promoters
Zhou et al., Inflamm Res 2007 : ( 3 ) LXA ( 4 ) restrained LPS induced IkappaBalpha degradation, NF-kappaB translocation, and NF-kappaB transcriptional activity
Wang et al., Cancer Lett 2007 (Neoplasm Invasiveness...) : CHM-1 also suppressed HGF induced Akt phosphorylation, and NF-kappaB activation, the downstream regulators of HGF/c-Met signaling, resulting in the inhibition of MMP-9 ... CHM-1 also suppressed HGF induced Akt phosphorylation, and NF-kappaB activation, the downstream regulators of HGF/c-Met signaling, resulting in the inhibition of MMP-9 ... CHM-1 also suppressed HGF induced Akt phosphorylation, and NF-kappaB activation, the downstream regulators of HGF/c-Met signaling, resulting in the inhibition of MMP-9
Lou et al., J Biol Chem 2007 : Here we demonstrate in the same system that GSH depletion down-regulates TNF induced NF-kappaB transactivation via two mechanisms, depending on the extent of the depletion ... With moderate GSH depletion ( approximately 50 % ), the down-regulation is IkappaB kinase (IKK) independent and likely acts on NF-kappaB transcriptional activity because TNF induced IKK activation, IkappaBalpha phosphorylation and degradation, NF-kappaB nuclear translocation, NF-kappaB DNA binding in vitro, and NF-kappaB subunit RelA ( p65 ) recruitment to kappaB sites of target gene promoters all appear unaltered
Takami et al., Arterioscler Thromb Vasc Biol 2007 (Carotid Artery Injuries...) : Overexpression of the UCHL1 gene significantly attenuated tumor necrosis factor (TNF)-alpha induced NF-kappaB activity in vascular cells and increased inhibitor of kappa B-alpha ( IkappaB-alpha ), possibly through the attenuation of IkappaB-alpha ubiquitination, leading to decreased neointima in the balloon injured artery ... Overexpression of the UCHL1 gene significantly attenuated tumor necrosis factor (TNF)-alpha induced NF-kappaB activity in vascular cells and increased inhibitor of kappa B-alpha ( IkappaB-alpha ), possibly through the attenuation of IkappaB-alpha ubiquitination, leading to decreased neointima in the balloon injured artery
Laumen et al., Atherosclerosis 2008 : In conclusion our data suggest that rosuvastatin inhibits PAI-1 expression and release from human adipocytes via a MEKK-1 dependent but not a NFkappaB dependent mechanism
Hsu et al., Cell Signal 2007 : Ultrasound induces cyclooxygenase-2 expression through integrin, integrin linked kinase, Akt, NF-kappaB and p300 pathway in human chondrocytes
Wang et al., Cell Signal 2007 (Pancreatic Neoplasms) : Receptor interacting protein ( RIP ) has been reported in TRAIL induced activation of NF-kappaB and we show here that knockdown of RIP sensitized the resistant cells to TRAIL induced apoptosis
Walsh et al., Shock 2008 : Activation of nuclear factor (NF)-kappaB is mediated by signal induced phosphorylation of IkappaBalpha , subsequent IkappaBalpha degradation, and then translocation of unbound NF-kappaB to the nucleus ... We found that inhibition of exportin 1 causes nuclear accumulation of both endogenous NF-kappaB ( Rel A ) and IkappaBalpha ... IL-1beta causes nuclear accumulation of NF-kappaB ( Rel A ) but does not increase nuclear IkappaBalpha
Jeong et al., APMIS 2007 (Stomach Neoplasms) : Additionally, diverse biologic roles of FLASH, including TNF induced NF-kappaB activation, cell-cycle progression and cell division, have been identified
Takarada et al., Eur J Pharmacol 2007 : In pre-osteoclastic cell line RAW264.7 cells, ascorbic acid similarly inhibited the formation of TRAP positive multinucleated cells, with a significant decrease in RANKL induced NF-kappaB transactivation
Choi et al., Eur J Pharmacol 2007 : Isoeugenol suppression of inducible nitric oxide synthase expression is mediated by down-regulation of NF-kappaB , ERK1/2, and p38 kinase ... The isoeugenol analogues eugenol and allylbenzene also inhibited LPS induced NF-kappaB signaling and iNOS expression, albeit with less potency than isoeugenol ... These results suggest that isoeugenol and its analogues inhibit NO production and iNOS expression in LPS stimulated RAW 264.7 cells, and that these effects are mediated , at least in part, by blocking the phosphorylation of ERK1/2 and p38 kinase, degradation of I-kappaBalpha, and activation of NF-kappaB ... These results suggest that isoeugenol and its analogues inhibit NO production and iNOS expression in LPS stimulated RAW 264.7 cells, and that these effects are mediated, at least in part, by blocking the phosphorylation of ERK1/2 and p38 kinase, degradation of I-kappaBalpha, and activation of NF-kappaB ... These results suggest that isoeugenol and its analogues inhibit NO production and iNOS expression in LPS stimulated RAW 264.7 cells, and that these effects are mediated, at least in part, by blocking the phosphorylation of ERK1/2 and p38 kinase, degradation of I-kappaBalpha , and activation of NF-kappaB
Souvannavong et al., Infect Immun 2007 : These results suggest that sustained stimulation with LPS can activate NF-kappaB through both classical and alternative pathways
Gealy et al., J Gen Virol 2007 (Glioblastoma) : As the interleukin-6 (IL-6) promoter can be activated by both NF-kappaB and IE2p86, it was examined whether there is competition between these two factors
Matteucci et al., Mol Cancer Res 2007 (Breast Neoplasms...) : Thus, the CXCR4 induction by HGF in MCF-7 cells required NF-kappaB and Ets1 activations, downstream of phosphoinositide-3-kinase/Akt, whereas in HGF treated MDA-MB231 cells, HDAC3 activation via c-Src probably caused a reduction of transcription factor activities, such as that of NF-kappaB ... Thus, the CXCR4 induction by HGF in MCF-7 cells required NF-kappaB and Ets1 activations, downstream of phosphoinositide-3-kinase/Akt, whereas in HGF treated MDA-MB231 cells, HDAC3 activation via c-Src probably caused a reduction of transcription factor activities, such as that of NF-kappaB
Caposio et al., J Biol Chem 2007 : Support for this finding comes from the observation that IFI16 targeting with specific short hairpin RNA down-regulates NF-kappaB binding activity to its cognate DNA and inhibits ICAM-1 expression induced by IFN-gamma ... Using transient transfection and luciferase assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation, we demonstrate indeed that activation of the NF-kappaB response is mediated by IFI16 induced block of Sp1-like factor recruitment to the promoter of the IkappaBalpha gene, encoding the main NF-kappaB inhibitor
Iikura et al., Lab Invest 2007 : IL-33 binds to the receptor, T1/ST2/IL-1R4, and can promote cytokine secretion by Th2 cells and NF-kappaB phosphorylation in mouse mast cells
Gonzalez-Navarrete et al., Gynecol Endocrinol 2007 : However, NF-kappaB was activated by TNF-alpha
Wang et al., Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2007 (Chronic Disease...) : It indicates the involvement of ERK, p38MAPK and NF-kappaB signal transduction pathway in regulation of COX-2 in CRS
Zhu et al., Curr Biol 2007 (Glaucoma) : Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP ... Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP ... Optineurin negatively regulates TNFalpha- induced NF-kappaB activation by competing with NEMO for ubiquitinated RIP ... NF-kappaB essential modulator (NEMO), the regulatory subunit of the IkappaB kinase (IKK) that activates NF-kappaB , is essential for NF-kappaB activation ... NF-kappaB essential modulator (NEMO) , the regulatory subunit of the IkappaB kinase (IKK) that activates NF-kappaB, is essential for NF-kappaB activation ... NEMO was recently found to contain a region that preferentially binds Lys ( K ) 63-linked but not K48 linked polyubiquitin ( polyUb ) chains, and the ability of NEMO to bind to K63 linked polyUb RIP ( receptor interacting protein ) is necessary for efficient tumor necrosis factor alpha (TNFalpha) induced NF-kappaB activation ... Although optineurin shares considerable homology with NEMO, in resting cells, it is not present in the high-molecular-weight complex containing IKKalpha and IKKbeta, and optineurin can not substitute for NEMO in lipopolysaccharide (LPS) induced NF-kappaB activation ... Optineurin competitively antagonized NEMO 's binding to polyUb RIP, and its overexpression inhibited TNFalpha induced NF-kappaB activation ... Optineurin competitively antagonized NEMO 's binding to polyUb RIP, and its overexpression inhibited TNFalpha induced NF-kappaB activation ... This competition occurs at physiologic protein levels because microRNA silencing of optineurin resulted in markedly enhanced TNFalpha induced NF-kappaB activity ... This competition occurs at physiologic protein levels because microRNA silencing of optineurin resulted in markedly enhanced TNFalpha induced NF-kappaB activity
Haugen et al., Endocrinology 2007 : NF-kappaB activation by globular adiponectin was not affected by antibody inhibition of toll-like receptor 4 or TNF receptors 1 and 2 but was attenuated by inhibitors of p38 MAPK , phosphatidylinositol 3-kinase, and protein kinase C. Analyses of the p65 subunit of NF-kappaB in different leukocyte cell lines showed activation of two monocytic cell lines ( U937 and THP-1 ) by native and globular adiponectin ... NF-kappaB activation by globular adiponectin was not affected by antibody inhibition of toll-like receptor 4 or TNF receptors 1 and 2 but was attenuated by inhibitors of p38 MAPK, phosphatidylinositol 3-kinase, and protein kinase C. Analyses of the p65 subunit of NF-kappaB in different leukocyte cell lines showed activation of two monocytic cell lines ( U937 and THP-1 ) by native and globular adiponectin ... NF-kappaB activation by globular adiponectin was not affected by antibody inhibition of toll-like receptor 4 or TNF receptors 1 and 2 but was attenuated by inhibitors of p38 MAPK, phosphatidylinositol 3-kinase, and protein kinase C. Analyses of the p65 subunit of NF-kappaB in different leukocyte cell lines showed activation of two monocytic cell lines ( U937 and THP-1 ) by native and globular adiponectin
Yamauchi et al., Int Immunol 2007 (Genetic Predisposition to Disease...) : Thus, Ifi202 is an important NF-kappaB activator in DCs and involved in IL-12 production, which may account for a T ( h ) 1-prone phenotype of BWF1 mice ... Thus, we hypothesized that Ifi202 is involved in elevated IL-12 production from BWF1 BMDCs. Overexpression of Ifi202 enhanced the LPS induced IkappaB-zeta, IL-12p40 and NF-kappaB promoter activities, while anti-sense ( AS ) RNA against Ifi202 strongly suppressed them in a monocytic cell line, RAW 264.7
Fang et al., J Med Virol 2007 (Severe Acute Respiratory Syndrome) : Expression of M suppressed tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation using a luciferase reporter assay
Ishiguro et al., Biochem Biophys Res Commun 2007 (Stomach Neoplasms) : 6-Gingerol was shown to down-regulate the expression of cIAP1, which suppresses caspase-3/7 activity, by inhibiting TRAIL induced NF-kappaB activation ... These findings indicate for the first time that in gastric cancer cells, 6-gingerol enhances TRAIL induced viability reduction by inhibiting TRAIL induced NF-kappaB activation while 6-shogaol alone reduces viability by damaging microtubules
Hutti et al., Mol Cell Biol 2007 : IkappaB kinase beta (IKKbeta) is an essential activator of NF-kappaB and is known to phosphorylate the NF-kappaB inhibitor, IkappaBalpha, allowing it to undergo ubiquitin mediated proteasomal degradation
Shibata et al., J Immunol 2007 (Colitis...) : The aim of the present study was to investigate whether the NF-kappaB essential modulator (NEMO) binding domain ( NBD ) peptide, which has been shown to block the association of NEMO with the IkappaB kinasebeta subunit (IKKbeta) and inhibit NF-kappaB activity, reduces inflammatory injury in mice with colitis ... The aim of the present study was to investigate whether the NF-kappaB essential modulator (NEMO) binding domain ( NBD ) peptide, which has been shown to block the association of NEMO with the IkappaB kinasebeta subunit (IKKbeta) and inhibit NF-kappaB activity, reduces inflammatory injury in mice with colitis
Liu et al., J Immunol 2007 : NF-kappaB signaling regulates functional expression of the MHC class I-related neonatal Fc receptor for IgG via intronic binding sequences ... Together, these data provide the first evidence that NF-kappaB signaling via intronic sequences regulates FcRn expression and function
Bengmark et al., JPEN J Parenter Enteral Nutr 2007 (Diabetes Mellitus...) : RAGE , a member of the immunoglobulin superfamily of cell surface molecules and receptor for advanced glycation end products, known since 1992, functions as a master switch, induces sustained activation of nuclear factor kappaB (NFkappaB) , suppresses a series of endogenous autoregulatory functions, and converts long lasting proinflammatory signals into sustained cellular dysfunction and disease
Zhang et al., Am J Pathol 2007 (Brain Ischemia...) : In conclusion, our results demonstrate that after MCAO the interaction between tPA and LRP results in NF-kappaB activation in astrocytes and induction of inducible nitric-oxide synthase expression in the ischemic tissue, suggesting a cytokine-like plasminogen independent role for tPA during cerebral ischemia ... In conclusion, our results demonstrate that after MCAO the interaction between tPA and LRP results in NF-kappaB activation in astrocytes and induction of inducible nitric-oxide synthase expression in the ischemic tissue, suggesting a cytokine-like plasminogen independent role for tPA during cerebral ischemia ... In conclusion, our results demonstrate that after MCAO the interaction between tPA and LRP results in NF-kappaB activation in astrocytes and induction of inducible nitric-oxide synthase expression in the ischemic tissue, suggesting a cytokine-like plasminogen independent role for tPA during cerebral ischemia
Wong et al., Immunol Lett 2007 (Hypersensitivity...) : The upregulation of costimulation induced IL-25 receptors and release of cytokines and chemokines from IL-25 treated costimulated Th cells were differentially regulated by intracellular JNK, p38 MAPK and NF-kappaB activity
Kelleher et al., J Biol Chem 2007 (Inflammation) : NOS2 regulation of NF-kappaB by S-nitrosylation of p65
Muddasani et al., J Biol Chem 2007 : Taken together, our results suggest that elevated expression of MMP-13 occurs through Elk-1 activation of both MAPK and NFkappaB signaling pathways, thus revealing a two pronged biological mechanism by which bFGF controls the production of catabolic enzymes that are associated with excessive degradation of the cartilage matrix in degenerative joint diseases such as osteoarthritis
Yamamoto et al., J Exp Med 2007 : Trib1 is reportedly involved in the regulation of NF-kappaB and MAP kinases, as well as gene expression in vitro ... Microarray analysis showed that Trib1-deficient macrophages exhibited a dysregulated expression pattern of lipopolysaccharide-inducible genes, whereas TLR mediated activation of MAP kinases and NF-kappaB was normal
Mahanivong et al., Oncogene 2008 (Neoplasm Invasiveness...) : Although constitutively active mutants of PKCalpha ( a cPKC ), PKCtheta ( a novel PKC ( nPKC ) ) and PKCzeta ( an atypical PKC ( aPKC ) ) were all able to activate NF-kappaB and upregulate uPA expression, only dominant negative PKCalpha mutant attenuated LPA induced NF-kappaB activation and uPA upregulation ... Although constitutively active mutants of PKCalpha ( a cPKC ), PKCtheta ( a novel PKC ( nPKC ) ) and PKCzeta ( an atypical PKC ( aPKC ) ) were all able to activate NF-kappaB and upregulate uPA expression, only dominant negative PKCalpha mutant attenuated LPA induced NF-kappaB activation and uPA upregulation ... These results suggest that PKCalpha, rather than PKC isoforms in other PKC classes, participates in LPA induced NF-kappaB activation and uPA upregulation in ovarian cancer cells
Kam et al., J Neurochem 2007 (Astrocytoma) : Activation of FPRL-1 also induced NFkappaB-driven luciferase expression
Rodriguez et al., Cell Signal 2007 : ROCK I-mediated activation of NF-kappaB by RhoB ... ROCK I-mediated activation of NF-kappaB by RhoB ... Here we show that RhoB can activate NF-kappaB signaling in multiple cell types ... Whereas RhoB-F is a potent activator of NF-kappaB , much weaker activation is observed for RhoB-GG, RhoA, and RhoC ... NF-kappaB activation by RhoB is not associated with increased nuclear translocation of RelA/p65, but rather, by modification of the RelA/p65 transactivation domain ... Activation of NF-kappaB by RhoB is dependent upon ROCK I but not PRK I ... Activation of NF-kappaB by RhoB is dependent upon ROCK I but not PRK I ... Thus, ROCK I cooperates with RhoB to activate NF-kappaB, and suppression of ROCK I activity by genetic or pharmacological inhibitors blocks NF-kappaB activation ... Thus, ROCK I cooperates with RhoB to activate NF-kappaB, and suppression of ROCK I activity by genetic or pharmacological inhibitors blocks NF-kappaB activation ... Suppression of RhoB activity by dominant-inhibitory mutants, or siRNA, blocks NF-kappaB activation by Bcr , and TSG101, but not by TNFalpha or oncogenic Ras ... Suppression of RhoB activity by dominant-inhibitory mutants, or siRNA, blocks NF-kappaB activation by Bcr, and TSG101, but not by TNFalpha or oncogenic Ras
Sebban-Benin et al., Hum Mol Genet 2007 (Incontinentia Pigmenti) : Mutation of all these ubiquitination sites severely impaired NF-kappaB activation induced by stimulation with IL-1, LPS, Nod2/RICK or serum/LPA ... Mutation of all these ubiquitination sites severely impaired NF-kappaB activation induced by stimulation with IL-1 , LPS, Nod2/RICK or serum/LPA ... Mutation of all these ubiquitination sites severely impaired NF-kappaB activation induced by stimulation with IL-1, LPS , Nod2/RICK or serum/LPA ... Mutation of all these ubiquitination sites severely impaired NF-kappaB activation induced by stimulation with IL-1, LPS, Nod2/RICK or serum/LPA
Lee et al., Helicobacter 2007 (Helicobacter Infections) : Nontoxic dose of capsaicin inhibited H. pylori induced IL-8 production by gastric epithelial cells through the modulation of IkappaB-, NF-kappaB- , and IL-8 pathways
Lin et al., J Leukoc Biol 2007 : PlGF was also able to inhibit LPS induced IkappaBalpha phosphorylation and NF-kappaB activity
Xue et al., Arthritis Rheum 2007 (Arthritis, Rheumatoid...) : Furthermore, APC directly suppressed the production of tumor necrosis factor (TNF) and the activation of NF-kappaB and MAP kinase p38, and inhibitors of NF-kappaB or p38 reduced the production of MMP-9, suggesting that APC inhibits MMP-9 by blocking TNF, NF-kappaB, and p38 ... Furthermore, APC directly suppressed the production of tumor necrosis factor (TNF) and the activation of NF-kappaB and MAP kinase p38, and inhibitors of NF-kappaB or p38 reduced the production of MMP-9, suggesting that APC inhibits MMP-9 by blocking TNF, NF-kappaB, and p38 ... Furthermore, APC directly suppressed the production of tumor necrosis factor (TNF) and the activation of NF-kappaB and MAP kinase p38 , and inhibitors of NF-kappaB or p38 reduced the production of MMP-9, suggesting that APC inhibits MMP-9 by blocking TNF, NF-kappaB, and p38 ... Furthermore, APC directly suppressed the production of tumor necrosis factor (TNF) and the activation of NF-kappaB and MAP kinase p38, and inhibitors of NF-kappaB or p38 reduced the production of MMP-9, suggesting that APC inhibits MMP-9 by blocking TNF, NF-kappaB , and p38
Wyler et al., Protein Sci 2007 : When expressed in human cells, some of the selected molecules, despite their partial degradation, inhibited TNF-alpha mediated NF-kappaB activation while having no effect on the basal activity
Puccetti et al., Nat Rev Immunol 2007 : IDO and regulatory T cells : a role for reverse signalling and non-canonical NF-kappaB activation
Kim et al., Chem Biol Interact 2008 : In an attempt to link the NF-kappaB activation and COX-2 induction during mammary carcinogenesis, we have examined the effects of 12-O-tetradecanoylphorbol-13-acetate ( TPA ), a prototype tumor promoter and a mitogen, on NF-kappaB activation and COX-2 expression in the immortalized human breast epithelial cell line ( MCF10A ) ... These results suggest that both p38 and ERK MAPKs activates NF-kappaB signaling, which in turn induces COX-2 expression in TPA stimulated human mammary epithelial cells
Kang et al., Stem Cells Dev 2007 : Nuclear localization of NF-kappaB in response to TNF-alpha was evident in differentiated, but not undifferentiated, SNUhES3 cells ... On the basis of an IkappaB kinase (IKK) inhibitor study, expression of IL-8 induced by TNF-alpha was dependent on NF-kappaB activity
Wang et al., Cancer Cell 2007 (Carcinoma, Squamous Cell...) : LZAP directly bound to RelA, impaired serine 536 phosphorylation of RelA, increased HDAC association with RelA , inhibited basal and stimulated NF-kappaB transcriptional activity, and was found at the promoter of selective NF-kappaB-responsive genes
Saito et al., Cancer Cell 2007 (Lymphoma, B-Cell...) : Activation of the CD40 receptor leads to NF-kappaB mediated induction of the IRF4 transcription factor , which, in turn, represses BCL6 expression by binding to its promoter region
Li et al., Circulation 2007 (Disease Models, Animal...) : In transgenic mice with cardiac-specific expression of a dominant negative mutant of IkappaB alpha ( IkappaB alpha ( S32A, S36A ) ), the upregulation of phosphorylated IkappaB alpha , activation of NF-kappaB , and cardiac COX-2 protein expression 3 days after iNOS gene therapy were abrogated, which was associated with the abolishment of the cardioprotective effects afforded by iNOS gene therapy ... In transgenic mice with cardiac-specific expression of a dominant negative mutant of IkappaB alpha ( IkappaB alpha ( S32A, S36A ) ), the upregulation of phosphorylated IkappaB alpha, activation of NF-kappaB , and cardiac COX-2 protein expression 3 days after iNOS gene therapy were abrogated, which was associated with the abolishment of the cardioprotective effects afforded by iNOS gene therapy
Jana et al., J Immunol 2007 : Involvement of NF-kappaB activation in LPS-, Abeta-, PrP-, poly IC-, Tat- , and MPP+-, but not IFN-gamma-, induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha ... Involvement of NF-kappaB activation in LPS-, Abeta-, PrP-, poly IC-, Tat-, and MPP+-, but not IFN-gamma- , induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha ... Involvement of NF-kappaB activation in LPS- , Abeta-, PrP-, poly IC-, Tat-, and MPP+-, but not IFN-gamma-, induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha ... Involvement of NF-kappaB activation in LPS-, Abeta-, PrP-, poly IC-, Tat-, and MPP+- , but not IFN-gamma-, induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha ... Involvement of NF-kappaB activation in LPS-, Abeta-, PrP-, poly IC-, Tat-, and MPP+-, but not IFN-gamma-, induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha ... Involvement of NF-kappaB activation in LPS-, Abeta-, PrP-, poly IC-, Tat-, and MPP+-, but not IFN-gamma-, induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha ... Involvement of NF-kappaB activation in LPS-, Abeta-, PrP- , poly IC-, Tat-, and MPP+-, but not IFN-gamma-, induced microglial expression of iNOS and stimulation of IkappaBalpha expression and inhibition of NF-kappaB activation by gemfibrozil via the PI3K pathway suggests that gemfibrozil inhibits the activation of NF-kappaB and the expression of proinflammatory molecules in microglia via PI3K mediated up-regulation of IkappaBalpha
von Bülow et al., J Immunol 2007 (MAP Kinase Signaling System) : Zinc dependent suppression of TNF-alpha production is mediated by protein kinase A-induced inhibition of Raf-1, I kappa B kinase beta, and NF-kappa B ... By this mechanism, zinc suppressed LPS induced activation of IkappaB kinase beta (IKKbeta) and NF-kappaB , and subsequent TNF-alpha production
Zhang et al., Neoplasia (New York, N.Y.) 2007 : We also used bioluminescence imaging of hollow fibers to monitor the nuclear factor kappaB (NFkappaB) pathway in vivo ; NFkappaB activation by lipopolysaccharide and tumor necrosis factor-alpha was evaluated in tumor cell lines genetically engineered to express luciferase controlled by an NFkappaB-responsive element
Pan et al., Int J Oncol 2007 (Bile Duct Neoplasms...) : The inverse activities of Fas and FasL promoter in tumor cells are regulated by NF-kappaB , which inhibits Fas expression and increases FasL expression through binding to their respective promoters ... The inverse activities of Fas and FasL promoter in tumor cells are regulated by NF-kappaB , which inhibits Fas expression and increases FasL expression through binding to their respective promoters
Suzuki et al., J Cell Physiol 2008 (Hemorrhage) : Two hrs thereafter, Kupffer cells and SMPhi production of IL-6, TNF-alpha, and IL-10, activation of MAPK ( p38, ERK-1/2, and JNK ), and transcription factors ( NF-kappaB and AP-1 ) were determined
Kim et al., PLoS Biol 2007 : We conclude that an inhibitory effect of AP-1 and STAT on NF-kappaB is required for properly balanced immune responses and appears to be evolutionarily conserved ... We conclude that an inhibitory effect of AP-1 and STAT on NF-kappaB is required for properly balanced immune responses and appears to be evolutionarily conserved
Greten et al., Cell 2007 (Disease Models, Animal...) : IKKbeta dependent NF-kappaB activation plays a key role in innate immunity and inflammation, and inhibition of IKKbeta has been considered as a likely anti-inflammatory therapy ... These results unravel an unanticipated role for IKKbeta dependent NF-kappaB signaling in the negative control of IL-1beta production and highlight potential complications of long-term IKKbeta inhibition
Girart et al., J Immunol 2007 (Melanoma) : Also, secretion of IFN-gamma was dependent on MEK1/ERK, p38 MAPK, p70 ( S6 ) kinase, and NF-kappaB , but not on calcineurin
Gallagher et al., J Neurosci 2007 : CNTF induced SYK phosphorylation is rapidly followed by increased tyrosine phosphorylation of IkappaB-alpha, and blocking SYK activation or tyrosine phosphorylation of IkappaB-alpha prevents CNTF induced NF-kappaB activation and CNTF promoted neurite growth ... CNTF induced SYK phosphorylation is rapidly followed by increased tyrosine phosphorylation of IkappaB-alpha , and blocking SYK activation or tyrosine phosphorylation of IkappaB-alpha prevents CNTF induced NF-kappaB activation and CNTF promoted neurite growth ... CNTF induced SYK phosphorylation is rapidly followed by increased tyrosine phosphorylation of IkappaB-alpha, and blocking SYK activation or tyrosine phosphorylation of IkappaB-alpha prevents CNTF induced NF-kappaB activation and CNTF promoted neurite growth
Guzman et al., Blood 2007 (Leukemia, Myeloid, Acute) : Molecular studies indicate the prevalent activities of DMAPT include induction of oxidative stress responses, inhibition of NF-kappaB , and activation of p53
Zuscik et al., Environ Health Perspect 2007 : Although Pb had no effect on basal CREB or Wnt/beta-catenin pathway activity, it induced NFkappaB signaling and inhibited AP-1 signaling ... Although Pb had no effect on basal CREB or Wnt/beta-catenin pathway activity, it induced NFkappaB signaling and inhibited AP-1 signaling ... Although Pb had no effect on basal CREB or Wnt/beta-catenin pathway activity, it induced NFkappaB signaling and inhibited AP-1 signaling ... Although Pb had no effect on basal CREB or Wnt/beta-catenin pathway activity, it induced NFkappaB signaling and inhibited AP-1 signaling
Ohno et al., Oncol Res 2005 : DHMEQ inhibited TNF-alpha-, IL-1beta-, and LPS induced NF-kappaB activation in HUVEC
Lee et al., Biochim Biophys Acta 2007 : HB-EGF induces delayed STAT3 activation via NF-kappaB mediated IL-6 secretion in vascular smooth muscle cell ... Furthermore, these HB-EGF mediated up-regulation of IL-6 mRNA expression and secretion were inhibited by NF-kappaB inhibitor Bay117082 ( 2.5 microM ) treatment suggesting involvement of NF-kappaB pathway
Wang et al., Biochim Biophys Acta 2007 : We further show that overexpression of caspase-10g dramatically enhances NF-kappaB activity in a dose- and time dependent manner
Criswell et al., J Biol Chem 2007 : This loss of E-cadherin was due to increased NFkappaB activity that, in turn, resulted in increased expression of the transcriptional repressors of E-cadherin such as Snail, Slug, Twist, and Sip1 ... These data indicate that TbetaRIII acts as a tumor suppressor in nontumorigenic mammary epithelial cells at least in part by inhibiting NFkappaB mediated repression of E-cadherin
Fang et al., Exp Eye Res 2007 : Inhibition of p42/44 activity by PD98059 significantly reduced LA-induced activation of NF-kappaB ... Inhibition of p42/44 activity by PD98059 significantly reduced LA-induced activation of NF-kappaB ... Linoleic acid induced expression of iNOS and COX-2 as well as PGE ( 2 ) and NO release in RPE cells were sequentially mediated through activation of p42/p44, MAPK, then NF-kappaB ... Linoleic acid induced expression of iNOS and COX-2 as well as PGE ( 2 ) and NO release in RPE cells were sequentially mediated through activation of p42/p44, MAPK, then NF-kappaB
Tsai et al., Toxicon 2007 : Moreover, LPS induced activation of NFkappaB was dramatically abolished, while phosphorylation and degradation of IkappaB were also inhibited
Gonzalez-Rey et al., Brain Behav Immun 2008 (Inflammation) : VIP downregulates NFkappaB dependent gene activation of the COX-2 promoter
Hybiske et al., Am J Physiol Lung Cell Mol Physiol 2007 (Calcium Signaling...) : We tested whether cystic fibrosis ( CF ) airway epithelia have larger innate immune responses than non-CF or cystic fibrosis transmembrane conductance regulator ( CFTR ) -corrected cells, perhaps resulting from ER stress due to retention of DeltaF508CFTR in the endoplasmic reticulum ( ER ) and activation of cytosolic Ca ( 2+ ) ( Ca(i) ) and nuclear factor (NF)-kappaB signaling
Kawauchi et al., Biol Pharm Bull 2007 : We have previously reported that cPrG.HCl suppressed the transcriptional activation of nuclear factor (NF)-kappaB
Kang et al., Biol Pharm Bull 2007 : Cornuside treatment attenuated tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor-kappa B (NF-kappaB) p65 translocation in HUVECs
Safina et al., Oncogene 2008 (Lung Neoplasms...) : TAK1 mediated regulation of MMP-9 involves NF-kappaB signaling ... Dn-TAK1 reduces NF-kappaB transcriptional response and inhibition of NF-kappaB reduces expression of MMP-9 and activity of the MMP-9 promoter reporter ... Dn-TAK1 reduces NF-kappaB transcriptional response and inhibition of NF-kappaB reduces expression of MMP-9 and activity of the MMP-9 promoter reporter
Qu et al., J Huazhong Univ Sci Technolog Med Sci 2007 : To investigate the role of NF-kappaB in TNF-alpha induced apoptosis in HSC-T6, a mutant IkappaBalpha was transfected into HSC-T6 cells by lipofectin transfection technique and its transient effect was examined 48 h after the transfection ... Our results showed that TNF-alpha could activate NF-kappaB in untransfected cells but not in transfected HSC-T6 cells
Jańczyk et al., Exp Dermatol 2007 : In addition, PL inhibited the SSR mediated transcriptional activation of NF-kappaB and AP1
Lee et al., Clin Exp Allergy 2007 (Asthma) : IL-8 promoter deletion analysis indicated that position -132 to +41 was essential for GCE induced IL-8 transcription, and mutants with substitutions in activator protein (AP)-1, nuclear factor (NF)-IL6 and NF-kappaB binding sites revealed a requirement for NF-kappaB and NF-IL6, but not AP-1, in GCE induced activation of the IL-8 promoter
Lantner et al., Blood 2007 : Stimulation of cell surface CD74 leads to NF-kappaB activation, which enables entry of the stimulated B cells into the S phase, induction of DNA synthesis, and cell division, and augments the expression of survival genes
Chae et al., Immunopharmacol Immunotoxicol 2007 (Second Messenger Systems) : Both NF-kappaB inhibitors had a larger inhibitory effect on the activity of cathepsin B in the presence of SB203580 ... However, SB203580 had no effect on the IL-6 induced activation of NF-kappaB , and neither of the NF-kappaB inhibitors decreased the level of p38 MAPK activation in the IL-6 stimulated osteoblasts
Sudheerkumar et al., Mol Carcinog 2008 (Brain Neoplasms...) : TNF-alpha induced NF-kappaB activation independent of PI3K/Akt pathway was also confirmed in human glioma cell lines-LN-229 and U373MG
Huang et al., Molecules (Basel, Switzerland) 2007 : Compounds 1-6 displayed moderate inhibition of tumor necrosis factor alpha (TNF-alpha) induced nuclear factor-kappaB (NF-kappaB) activation, while 2 and 4 displayed potential inhibitory activity toward proteasome chymotripsin-like activation
Kim et al., Cytokine 2007 : Quercetin 3-O-beta- ( 2''-galloyl ) -glucopyranoside inhibits endotoxin LPS induced IL-6 expression and NF-kappa B activation in macrophages ... Taken together, QG-32 could inhibit NF-kappaB dependent IL-6 expression, targeting nuclear translocation of NF-kappaB complex downstream IkappaB degradation
Lin et al., J Pharmacol Exp Ther 2007 (Neuroblastoma) : GLNVA also reduced cyclooxygenase (COX)-2 expression, inhibitor of nuclear factor (NF)-kappaB ( IkappaB)alpha/IkappaBbeta degradation, NF-kappaB activation , and the overproduction of tumor necrosis factor-alpha , interleukin (IL)-1beta, and prostaglandin E2 in BV-2 cells ... GLNVA also reduced cyclooxygenase (COX)-2 expression, inhibitor of nuclear factor (NF)-kappaB ( IkappaB)alpha/IkappaBbeta degradation, NF-kappaB activation , and the overproduction of tumor necrosis factor-alpha, interleukin (IL)-1beta , and prostaglandin E2 in BV-2 cells ... GLNVA also reduced cyclooxygenase (COX)-2 expression, inhibitor of nuclear factor (NF)-kappaB ( IkappaB)alpha/IkappaBbeta degradation, NF-kappaB activation , and the overproduction of tumor necrosis factor-alpha, interleukin (IL)-1beta , and prostaglandin E2 in BV-2 cells
El Mjiyad et al., J Virol 2007 : VZV also inhibits icam-1 stimulation of TNF-alpha by strongly reducing NF-kappaB nuclear translocation in MRC5 fibroblasts ... VZV also inhibits icam-1 stimulation of TNF-alpha by strongly reducing NF-kappaB nuclear translocation in MRC5 fibroblasts
Lee et al., Toxicol Appl Pharmacol 2007 (Colonic Neoplasms...) : Also, 0.02 % UDN glycoprotein suppressed the DNA binding activities of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1), accompanying the inhibitions of its subunits ( p50, p65, c-Jun, and c-Fos ), pro-inflammatory proteins [ inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) ], and pro-inflammatory cytokines [ tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 ] on DMH stimulated ACF formation
Park et al., Mol Cell Endocrinol 2007 : In chicken UMNSAH/DF-1 cells, nuclear factor kappaB (NF-kappaB) was activated in an FKBP51 dependent manner
Shinohara et al., Arterioscler Thromb Vasc Biol 2007 (Carotid Artery Diseases...) : Moreover, transfection of TLR2 and TLR4 resulted in synergistic activation of NF-kappaB at the vessel wall in vivo, and in vascular smooth muscle cells in vitro ... Moreover, transfection of TLR2 and TLR4 resulted in synergistic activation of NF-kappaB at the vessel wall in vivo, and in vascular smooth muscle cells in vitro
Wong et al., Am J Physiol Lung Cell Mol Physiol 2007 : Decreased levels of IkappaB-alpha in airway cells exposed to ricin suggest that translational suppression may be responsible for the activation of NF-kappaB
Yu et al., J Mol Neurosci 2007 : Rather, blockade of proteasome activity, as well as inhibition of NFkappaB activation, reduced the basal expression of LIFR ... Thus, NFkappaB activity and proteasome degradation of IkappaB stabilized LIFR and prevented its rapid lysosomal degradation
Smolinska et al., Mol Immunol 2008 : Interestingly, however, PP2 had no significant effect on the activation of NF-kappaB , or on p42/44 ERK, p46/54 JNK or p38 MAPK phosphorylation
Lavon et al., Cancer Res 2007 (Glioma) : This prompted us to hypothesize that NF-kappaB might be involved in MGMT regulation ... The findings of our study strongly suggest that NF-kappaB plays a major role in MGMT regulation and that MGMT is most probably the major player in NF-kappaB mediated chemoresistance to alkylating agents
Mutoh et al., J Gastroenterol 2007 : The results indicate that CDX2 inhibits transcription of Cox-2 by interfering with the binding of NF-kappaB on the NF-kappaB binding site
Keating et al., J Biol Chem 2007 : The poxviral TLR antagonist A52 inhibited NFkappaB activation by TLR2 , -3, -4, -5, -7, and -9 ligands, via its interaction with IRAK-2, while not affecting interferon regulatory factor activation
Shibolet et al., J Biol Chem 2007 : By systematically integrating expression profiles from immune and epithelial cells with functional studies, we demonstrate that protein kinase A anchoring protein 13 ( AKAP13 ), a scaffold protein with GEF activity, is an activator of NF-kappaB downstream of TLR2 signaling ... Furthermore, NF-kappaB activation was partially inhibited by a GEF-deficient AKAP13 mutant ... Together, our results suggest that AKAP13 plays a role in TLR2 mediated NF-kappaB activation and suggest that GEF containing scaffold proteins may confer specificity to innate immune responses downstream of TLRs ... Together, our results suggest that AKAP13 plays a role in TLR2 mediated NF-kappaB activation and suggest that GEF containing scaffold proteins may confer specificity to innate immune responses downstream of TLRs
El Shikh et al., J Immunol 2007 : In short, we report expression of TLR4 on FDCs for the first time and that engagement of FDC-TLR4 activated NF-kappaB , up-regulated expression of molecules important in FDC accessory function, including Fc gammaRIIB, ICAM-1, and VCAM-1, as well as FDC accessory activity in promoting recall IgG responses
Qiao et al., J Immunol 2007 : Mechanistically, Cbl-b selectively down-modulates CD40 induced activation of NF-kappaB and JNK ... Together, our data suggest that Cbl-b attenuates CD40 mediated NF-kappaB and JNK activation, thereby suppressing B cell responses
Moulakakis et al., J Immunol 2007 (Inflammation) : The mechanisms underlying SP-A mediated inhibition of LPS induced NF-kappaB activation in vivo and in vitro are only partially understood ... We previously demonstrated that SP-A stabilizes IkappaB-alpha , the primary regulator of NF-kappaB , in alveolar macrophages ( AM ) both constitutively and in the presence of LPS
Rowland et al., J Immunol 2007 : Our previous experiments with TRAF2-deficient B cell lines suggest that TRAF6 and TRAF2 may have redundant roles in CD40 mediated NF-kappaB activation ... Consistent with this hypothesis, we found CD40 mediated activation of NF-kappaB intact in TRAF6-deficient cells and defective in cells lacking both TRAF2 and TRAF6
Liu et al., J Immunol 2007 : Activation of NF-kappaB , STAT6, and STAT1 was induced in eosinophils by TNF-alpha , IL-4, and IFN-gamma, respectively ... Activation of NF-kappaB , STAT6, and STAT1 was induced in eosinophils by TNF-alpha, IL-4, and IFN-gamma , respectively ... Activation of NF-kappaB , STAT6, and STAT1 was induced in eosinophils by TNF-alpha, IL-4 , and IFN-gamma, respectively
Fontalba et al., J Immunol 2007 (Arthritis, Rheumatoid) : Thus, CARD8 may control NF-kappaB activation through a regulatory loop ... Thus, CARD8 may control NF-kappaB activation through a regulatory loop ... Furthermore, in contrast to the full-length protein, CARD8-S did not modify the expression of NF-kappaB target genes ( cIAP, A1 ), in response to TNF-alpha
Madonna et al., Int J Immunopathol Pharmacol 2007 : Co-treatment of HUVEC with insulin and SB202190 strongly reverted the stimulatory effect of insulin on NF-KB activation, thus establishing a link between NF-KB activation and p38MAPkinase mediated induction of VCAM-1 by insulin ... Co-treatment of HUVEC with insulin and SB202190 strongly reverted the stimulatory effect of insulin on NF-KB activation, thus establishing a link between NF-KB activation and p38MAPkinase mediated induction of VCAM-1 by insulin ... Co-treatment of HUVEC with insulin and SB202190 strongly reverted the stimulatory effect of insulin on NF-KB activation, thus establishing a link between NF-KB activation and p38MAPkinase mediated induction of VCAM-1 by insulin
Thornburg et al., J Virol 2007 : Induction of epidermal growth factor receptor expression by Epstein-Barr virus latent membrane protein 1 C-terminal activating region 1 is mediated by NF-kappaB p50 homodimer/Bcl-3 complexes ... LMP1 has two domains that both activate NF-kappaB ... In this study, the role of NF-kappaB p50 and Bcl-3 in LMP1 mediated upregulation of EGFR was analyzed
Yokozawa et al., J Pharm Pharmacol 2007 (Disease Models, Animal...) : Furthermore, it inhibited the increase of cyclooxygenase (COX)-2 with the regulation of nuclear factorkappa B (NF-kappaB) and bcl-2 proteins in the liver, suggesting that the protective potential of Kangenkaryu extract against metabolic syndrome would be attributed to the regulation of COX-2, NF-kappaB, and bcl-2 signalling pathways ... Furthermore, it inhibited the increase of cyclooxygenase (COX)-2 with the regulation of nuclear factorkappa B (NF-kappaB) and bcl-2 proteins in the liver, suggesting that the protective potential of Kangenkaryu extract against metabolic syndrome would be attributed to the regulation of COX-2, NF-kappaB, and bcl-2 signalling pathways
Charbonneau et al., J Biol Chem 2007 (Anoxia...) : By the use of HIF-1-deficient cells and by obliterating NF-kappaB activation, it was determined that the hypoxic TACE response is mediated by HIF-1 signaling, whereas the regulation by TNFalpha also requires NF-kappaB activation
Choi et al., J Med Food 2007 : Furthermore, MFWE suppressed the LPS induced phosphorylations of p38 MAPK and extracellular signal regulated kinase MAPK, as well as IkappaBalpha degradation and NF-kappaB activation
Legendre et al., Clin Exp Rheumatol 2007 : The drug reduced IL-1Beta induced NF-kappaB and AP-1 DNA binding, as well as the phosphorylation of ERK and JNK
Andersson et al., J Cyst Fibros 2008 (Cystic Fibrosis...) : LPS induced TNFalpha and IL-6 secretion and NFkappaB p65 activity were increased in CF macrophages
Lee et al., J Endod 2007 : Furthermore, the eugenol modulated COX-2 expression was inhibited by an NF-kappaB inhibitor, N-acetylcysteine ... Taken together, eugenol might induce COX-2 expression through the activation of NF-kappaB in human osteoblasts
García et al., Ann Rheum Dis 2008 (Arthritis, Rheumatoid) : PARP inhibition reduced TNF induced JNK phosphorylation and AP-1 and NF kappaB binding activities were partially impaired by treatment with PARP inhibitors or by PARP-1 knockdown
Paukkeri et al., Br J Pharmacol 2007 : However, PPARalpha agonists did not alter LPS induced iNOS mRNA expression or activation of NF-kappaB or STAT1 which are important transcription factors for iNOS ... However, PPARalpha agonists did not alter LPS induced iNOS mRNA expression or activation of NF-kappaB or STAT1 which are important transcription factors for iNOS
Holden et al., Br J Pharmacol 2007 : Using BEAS-2B bronchial epithelial cells, NF-kappaB dependent transcription and both release and mRNA expression of IL-6 and IL-8 were examined by reporter assay, ELISA and quantitative RT-PCR ... Using BEAS-2B bronchial epithelial cells, NF-kappaB dependent transcription and both release and mRNA expression of IL-6 and IL-8 were examined by reporter assay, ELISA and quantitative RT-PCR ... Similarly, H ( 3 ) and H ( 4 ) receptor agonists did not affect TNFalpha induced NF-kappaB dependent transcription, or IL-6 and IL-8 release at concentrations below 10 microM ... Similarly, H ( 3 ) and H ( 4 ) receptor agonists did not affect TNFalpha induced NF-kappaB dependent transcription, or IL-6 and IL-8 release at concentrations below 10 microM ... The anti-inflammatory glucocorticoid, dexamethasone, inhibited the histamine enhanced NF-kappaB dependent transcription and IL-6 and IL-8 release ... The anti-inflammatory glucocorticoid, dexamethasone, inhibited the histamine enhanced NF-kappaB dependent transcription and IL-6 and IL-8 release
Chen et al., J Cell Biochem 2008 (Hypertrophy...) : In addition, LPA stimulated the phosphorylation of Akt and p65 protein and activated NF-kappaB-luciferase expression ... Furthermore, ERK inhibitors ( U0126 and PD98059 ) suppressed LPA stimulated activation of NF-kappaB and p65 phosphorylation whereas wortmannin showed no effect on NF-kappaB activation ... Furthermore, ERK inhibitors ( U0126 and PD98059 ) suppressed LPA stimulated activation of NF-kappaB and p65 phosphorylation whereas wortmannin showed no effect on NF-kappaB activation
Homma et al., J Interferon Cytokine Res 2007 : The Th2 transcription factor c-Maf inhibits IL-12p35 gene expression in activated macrophages by targeting NF-kappaB nuclear translocation ... The Th2 transcription factor c-Maf inhibits IL-12p35 gene expression in activated macrophages by targeting NF-kappaB nuclear translocation
Mozar et al., J Cell Physiol 2008 (Bone Resorption) : Moreover, we found that Pi significantly reduced the RANKL stimulated DNA binding activity of NF-kappaB
Hering et al., Ann Surg Oncol 2007 (Adenocarcinoma...) : An experimental paradigm to screen for potential contributory mechanism ( s ) in altered pancreatic cancer cellular proliferation was defined, and using this approach the co-administration of n-3 FA with GEM inhibited GEM induced NF-kB activation and restored apoptosis in the MIA PaCa-2 cell-line
Yamaguchi et al., J Biol Chem 2007 : However, down-regulation of USP11 dramatically enhances NF-kappaB activity in response to tumor necrosis factor-alpha, indicating that IKKalpha does not require activation of NF-kappaB ... However, down-regulation of USP11 dramatically enhances NF-kappaB activity in response to tumor necrosis factor-alpha, indicating that IKKalpha does not require activation of NF-kappaB ... However, down-regulation of USP11 dramatically enhances NF-kappaB activity in response to tumor necrosis factor-alpha , indicating that IKKalpha does not require activation of NF-kappaB
Srivastava et al., J Biol Chem 2007 : Overexpression of a dominant negative inhibitor of NF-kappaB or AP-1 blocked the TNF-alpha induced expression of MMP-9 in myotubes ... Furthermore, overexpression of a dominant negative mutant of TAK1 blocked the TNF-alpha induced expression of MMP-9 and activation of NF-kappaB and AP-1 ... Furthermore, overexpression of a dominant negative mutant of TAK1 blocked the TNF-alpha induced expression of MMP-9 and activation of NF-kappaB and AP-1
Leung et al., Nephrol Dial Transplant 2008 : Furthermore, the increased synthesis of IL-6 and TNF-alpha by anionic pIgA in HMC was significantly diminished ( P < 0.01 ) in the presence of NF-kappaB inhibitor pyrrolidine dithiocarbamate and NF-kappaB blocking permeable peptides SN50 ( P < 0.01 ) ... Furthermore, the increased synthesis of IL-6 and TNF-alpha by anionic pIgA in HMC was significantly diminished ( P < 0.01 ) in the presence of NF-kappaB inhibitor pyrrolidine dithiocarbamate and NF-kappaB blocking permeable peptides SN50 ( P < 0.01 ) ... While both the p42/p44 MAPK and NF-kappaB pathways are essential in regulating the anionic pIgA induced synthesis of IL-6, TNF-alpha synthesis mediated by anionic pIgA is partly dependent on NF-kappaB
Belaiba et al., Mol Biol Cell 2007 (Anoxia) : In line, expression of the NFkappaB subunits p50 and p65 enhanced HIF-1alpha mRNA levels, whereas blocking of NFkappaB by an inhibitor of nuclear factor-kappaB attenuated HIF-1alpha mRNA induction by hypoxia ... Together, these findings provide a novel mechanism in which hypoxia induces HIF-1alpha mRNA expression via the PI3K/AKT pathway and activation of NFkappaB ... Together, these findings provide a novel mechanism in which hypoxia induces HIF-1alpha mRNA expression via the PI3K/AKT pathway and activation of NFkappaB
Yadav et al., Invest Ophthalmol Vis Sci 2007 (Disease Models, Animal...) : AR inhibition also prevented the LPS induced activation of NF-kappaB and expression of COX-2 and iNOS in the human monocyte cell line U-937
Zhang et al., Ann Surg Oncol 2007 (Adenocarcinoma...) : There was a positive correlation between NF-kappaB activation ( nuclear translocation of RelA ) and tumor clinicopathological features such as tumor grade, including T stages, N stages, and tumor, node, metastasis system stages, smoking status, and age
Peng et al., J Gastrointest Surg 2007 (Sepsis...) : AdPKC-zeta DN attenuated the LPS induced upregulation of PKC-zeta activity, IKKbeta, IKKgamma, NF-kappaB , Fas/FasL, Caspase-3, and DNA fragmentation in Kupffer cells ( all p < 0.001 ), whereas overexpression of PKC-zeta augmented LPS induced upregulation of IKKbeta, IKKgamma, NF-kappaB, Caspase-3, and DNA fragmentation ( p < 0.001 )
Zhang et al., J Ethnopharmacol 2007 (Diabetes Mellitus, Experimental...) : In the present study, we investigated the effect of Astragalus polysaccharide (APS) , an aqueous extract from the Astragalus membranaceus roots, on gene expressions of NF-kappaB and an inhibitory protein of nuclear factor-kappaB ( IkappaB ) in experimental DN induced by streptozotocin in male Sprague-Dawley rats
Heo et al., Stem Cells 2007 (Calcium Signaling...) : Subsequently, Shh increased the nuclear factor (NF)-kappaB p65 phosphorylation, which was inhibited by blocking PKC and EGFR tyrosine kinase
Hsieh et al., J Cell Biochem 2008 : Furthermore, S1P induced EGFR upregulation was blocked by a selective NF-kappaB inhibitor helenalin
Caggiano et al., J Med Chem 2007 (Arthritis, Experimental...) : Estrogen receptor dependent inhibitors of NF-kappaB transcriptional activation-1 synthesis and biological evaluation of substituted 2-cyanopropanoic acid derivatives : pathway selective inhibitors of NF-kappaB, a potential treatment for rheumatoid arthritis
Bauer et al., Exp Cell Res 2007 (Melanoma...) : Rac1 stimulates NFkappaB , and we found that endogenous NFkappaB activity of FEMX-V cells was 2 % of that of FEMX-I cells ... Inhibition of Rac1 resulted in blocked NFkappaB activity
Higashitsuji et al., Biochem Biophys Res Commun 2007 : Gankyrin did not affect the NF-kappaB DNA binding activity or nuclear translocation of RelA induced by TNFalpha in these cells
Jung et al., J Ethnopharmacol 2007 : WHW extract attenuated the phosphorylation of mitogen activated protein kinases ( MAPKs ), extracellular signal regulated kinases 1 and 2 ( ERK1/2 ) and c-Jun N-terminal kinase (JNK) , as well as the activation of nuclear factor-kappa B (NF-kappaB) in LPS stimulated RAW264.7 cells
Bogatkevich et al., Arthritis Rheum 2007 (MAP Kinase Signaling System...) : Inhibition of MMP activity by MMP inhibitor GM1489 and inhibition of MMP-1 expression by siRNA did not prevent HGF induced ERK-1/2 phosphorylation and NF-kappaB activity, but significantly restored HGF inhibited collagen and CTGF accumulation ... Inhibition of MMP activity by MMP inhibitor GM1489 and inhibition of MMP-1 expression by siRNA did not prevent HGF induced ERK-1/2 phosphorylation and NF-kappaB activity, but significantly restored HGF inhibited collagen and CTGF accumulation
Dossumbekova et al., Arthritis Rheum 2007 : Cyclic tensile strain rapidly inhibited the IL-1beta induced nuclear translocation of NF-kappaB , but not its IL-1beta induced phosphorylation at serine 276 and serine 536, which are necessary for its transactivation and transcriptional efficacy, respectively
Hong et al., Cancer Res 2007 (Breast Neoplasms...) : In addition, Smad7 mediates the inhibitory activity of TGF-beta on TNF induced NF-kappaB activation and the synergistic activity of TGF-beta on TNF induced apoptosis
Ganguly et al., Immunol Cell Biol 2008 (Tuberculosis) : Mycobacterium tuberculosis secretory proteins CFP-10, ESAT-6 and the CFP10 : ESAT6 complex inhibit lipopolysaccharide induced NF-kappaB transactivation by downregulation of reactive oxidative species ( ROS ) production ... These proteins also downregulated the bacterial lipopolysaccharide (LPS) induced ROS production, which, in turn, downregulated LPS induced nuclear factor-kappaB (NF-kappaB) p65 DNA binding activity, as well as inhibited the NF-kappaB dependent reporter gene ( chloramphenicol acetyl transferase ) expression in the treated macrophages
Du et al., J Cell Biochem 2007 : This review focuses on the IFN induced NF-kappaB activation pathway and the role of NF-kappaB in ISG expression, antiviral activity and apoptosis, and the therapeutic application of IFN in cancer and infectious disease
Yang et al., J Cell Biochem 2008 : Consistent to these results, overexpression of GILZ also inhibits NF-kappaB mediated COX-2 promoter activity ... Finally, we show that GILZ inhibits COX-2 expression by blocking NF-kappaB nuclear translocation
Lalor et al., Ann N Y Acad Sci 2007 (Liver Diseases) : Engagement of VAP-1 results in PI3-kinase dependent NF-kappaB activation and increased chemokine and adhesion molecule expression
Mi et al., Mol Ther 2008 (Carcinoma, Non-Small-Cell Lung...) : This Dox induced NF-kappaB activation and subsequent chemoresistance is dependent on expression of p53
Song et al., Int J Mol Med 2007 : The molecular mechanism by which RAE inhibited iNOS gene expression appeared to involve the inhibition of NF-kappaB activation as a result of RAE 's suppression of IL-1beta and IFN-gamma induced IkappaBalpha degradation ... The molecular mechanism by which RAE inhibited iNOS gene expression appeared to involve the inhibition of NF-kappaB activation as a result of RAE 's suppression of IL-1beta and IFN-gamma induced IkappaBalpha degradation
Oh et al., J Biol Chem 2007 : Inhibition of the activity of Syk prior to, concomitant with or shortly following receptor engagement led to the rapid inhibition of receptor mediated tyrosine phosphorylation and blocked the activation of extracellular signal regulated kinase, NF-kappaB , and NFAT ... The receptor mediated activation of NF-kappaB required active Syk for a relatively short period of time, whereas the activation of NFAT required active kinase for a prolonged ( > 1 h ) period
Liu et al., Eur J Pharmacol 2007 (Inflammation) : In addition, ABL directly inhibited the binding of active NF-kappaB to specific DNA cis-element
Tang et al., Carcinogenesis 2008 (Lung Neoplasms...) : Both mitogen activated protein kinase kinase inhibitor and ERK mutant also antagonized SDF-1alpha induced NF-kappaB-driven luciferase promoter activity
Hostager et al., Immunol Res 2007 : TRAF6 contributes to the CD40 mediated activation of NF-kappaB , stress activated protein kinases, and perhaps other signaling molecules ... TRAF6 contributes to the CD40 mediated activation of NF-kappaB , stress activated protein kinases, and perhaps other signaling molecules
Yepes et al., Mol Neurobiol 2007 (Brain Edema...) : Furthermore, there is a growing body of evidence indicating that TWEAK induces the activation of the NF-kappaB in the CNS with release of proinflammatory cytokines and matrix metalloproteinases
Higai et al., Biol Pharm Bull 2007 : Nuclear factor kappaB (NFkappaB) and activator protein-1 (AP-1) reporter genes were also enhanced although CCAAT/enhancer binding protein (C/EBP) was not affected
D'Aversa et al., J Neurosci Res 2008 (AIDS Dementia Complex...) : Gel shift analyses demonstrated that NFkappaB and AP-1, but not C/EBPbeta, mediate microglial CXCL8 production
Park et al., Int Immunopharmacol 2007 (Bone Resorption) : However, SKE did not inhibit NF-kappaB activation by RANKL
Chaea et al., Int Immunopharmacol 2007 : Electrophoretic mobility shift assay ( EMSA ) and western blot analysis showed that YS-51S inhibited MIX mediated activation and translocation of NF-kappaB to nucleus by suppressing the degradation of its inhibitory protein IkappaBalpha in cytoplasm
Youn et al., Biochem Pharmacol 2008 : Our results showed that cinnamaldehyde suppressed the activation of NFkappaB and IRF3 induced by LPS , a TLR4 agonist, leading to the decreased expression of target genes such as COX-2 and IFNbeta in macrophages ( RAW264.7 ) ... Cinnamaldehyde did not inhibit the activation of NFkappaB or IRF3 induced by MyD88 dependent ( MyD88, IKKbeta ) or TRIF dependent ( TRIF, TBK1 ) downstream signaling components ... Further, cinnamaldehyde inhibited ligand independent NFkappaB activation induced by constitutively active TLR4 or wild-type TLR4
Mizobe et al., Exp Hematol 2007 (Cell Transformation, Viral) : In this study, we investigated whether MyD88 , an adaptor protein of Toll-like receptor ( TLR ) signaling, contributes to constitutive NF-kappaB activation in HTLV-I transformed T cells ... Constitutive activation of NF-kappaB and NF-IL-6 and cytokine gene promoters, such as IL-1alpha, interferon-gamma, and tumor necrosis factor-alpha in MT2 cells was inhibited by MyD88dn expression ... HTLV-I Tax enhanced TLR expression and synergistically activated NF-kappaB with wild-type MyD88
Cortez et al., Am J Physiol Heart Circ Physiol 2007 : IL-17 stimulated activator protein-1 [ AP-1 ( c-Fos, c-Jun, and Fra-1 ) ], NF-kappaB ( p50 and p65 ), and CCAAT enhancer binding protein (C/EBP)-beta DNA binding and reporter gene activities, effects attenuated by antisense oligonucleotides, siRNA mediated knockdown, or expression of dominant negative signaling proteins ... Inhibition of AP-1, NF-kappaB , or C/EBP activation attenuated IL-17 stimulated MMP-1 expression ... Our data indicate that IL-17 induces MMP-1 in human cardiac fibroblasts directly via p38 MAPK- and ERK dependent AP-1, NF-kappaB , and C/EBP-beta activation and suggest that IL-17 may play a critical role in myocardial remodeling
Yu et al., Methods Find Exp Clin Pharmacol 2007 (Endotoxemia) : LPS increased TLR4 expression and NF-kappa-B activity in the rat lungs
Hohensinner et al., Journal of thrombosis and haemostasis : JTH 2007 : To determine a possible role of nuclear factor kappaB (NF-kappaB) and activating protein 1 (AP-1) in M-CSF regulation, blockers to both pathways and an adenovirus overexpressing a dominant negative ( dn ) form of IkappaB kinase 2 (IKK2) were used
Chung et al., Toxicological sciences : an official journal of the Society of Toxicology 2008 (Inflammation) : In addition, the inhibition of 5-LO by AA861 markedly reduced AcQ induced nuclear factor kappa B (NF-kappa B) activation
Lim et al., PloS one 2007 (Haemophilus Infections...) : NTHi induced CYLD , in turn, negatively regulates NTHi induced NF-kappaB activation through deubiquitinating TRAF6 and 7 and down-regulates inflammation
Chen et al., Basic Clin Pharmacol Toxicol 2007 (Leukemia) : Furthermore, curcumin could also prevent degradation of I kappaB alpha and inhibit nuclear translocation of the NF-kappaB/p65 subunit, as well as expression of Notch 1, induced by tumour necrosis factor-alpha
Park et al., Biochem Biophys Res Commun 2007 (Embryo Loss) : TRAF interacting protein (TRIP) is required for the inhibitory regulation of TNF induced NF-kappaB signaling via the TNFR/TRAF signaling complexes in vitro ... TRAF interacting protein (TRIP) is required for the inhibitory regulation of TNF induced NF-kappaB signaling via the TNFR/TRAF signaling complexes in vitro ... TRIP also directly interacts with the familial cylindromatosis tumor suppressor gene ( CYLD ) and negatively regulates NF-kappaB activation in vitro
Schapansky et al., Exp Neurol 2007 (Alzheimer Disease) : NF-kappaB activated by ER calcium release inhibits Abeta mediated expression of CHOP protein : enhancement by AD-linked mutant presenilin 1 ... Further, specific inhibition of NF-kappaB resulted in greater expression of CHOP, while activation of NF-kappaB inhibited CHOP expression
Delgado André et al., Blood Cells Mol Dis 2008 : The transcription factor NF-kappaB is activated by PKR through phosphorylation and subsequent degradation of its inhibitor I-kappaBbeta
Kanda et al., Am J Physiol Cell Physiol 2007 : Histamine alone enhanced transcriptional activities of NF-kappaB and activator protein-1 (AP-1) and potentiated TNF-alpha induced NF-kappaB and AP-1 activities or IFN-gamma induced NF-kappaB and STAT1 activities ... Histamine alone enhanced transcriptional activities of NF-kappaB and activator protein-1 (AP-1) and potentiated TNF-alpha induced NF-kappaB and AP-1 activities or IFN-gamma induced NF-kappaB and STAT1 activities ... These results suggest that histamine stimulates H1 receptor and potentiates TNF-alpha- or IFN-gamma induced hBD-2 production dependent on NF-kappaB , AP-1, or STAT1 in human keratinocytes ... These results suggest that histamine stimulates H1 receptor and potentiates TNF-alpha- or IFN-gamma induced hBD-2 production dependent on NF-kappaB , AP-1, or STAT1 in human keratinocytes
Ackerman et al., Biol Reprod 2008 : We hypothesized that NFkappaB might regulate cytokine induced PTGES expression in amnion cells ... In conclusion, our results support a role for NFkappaB in cytokine induced PTGES expression in amnion mesenchymal cells in vitro
Jeong et al., Virology 2008 : Since AKT inhibitors simultaneously inhibit NF-kappaB and activate p53, these drugs should be promising candidates for HTLV-1 associated cancer therapy
Kim et al., J Ethnopharmacol 2007 (Atherosclerosis) : In lipopolysaccharide (LPS) stimulated RAW264.7 cells, HMCO5 inhibited NF-kappaB activation as well as iNOS promoter activity, inhibited the secretion of TNF-alpha and IL-1beta, and directly inhibited the intracellular accumulation of reactive oxygen species ... In lipopolysaccharide (LPS) stimulated RAW264.7 cells, HMCO5 inhibited NF-kappaB activation as well as iNOS promoter activity, inhibited the secretion of TNF-alpha and IL-1beta , and directly inhibited the intracellular accumulation of reactive oxygen species
Doyle et al., J Biol Chem 2007 (Agammaglobulinemia...) : We demonstrate that Btk is required for NFkappaB activation, participating in the pathway to increased phosphorylation of p65 on serine 536 activated by TLR8 and TLR9
Ying-fang et al., Indian J Med Res 2007 : At this optimum concentration, PAF significantly increased the cell proliferation index ( PI ) and the PCNA positive rate in the ASMCs, as well as NF-kappaB DNA- binding activity
Frelin et al., Cell Death Differ 2008 : This short form was unable to rescue NF-kappaB activation by tumor necrosis factor-alpha (TNF-alpha) when transfected in NEMO-deficient cells
Ravi et al., Mol Cell Biochem 2008 (Melanoma) : We also show that caffeine specifically inhibits UV-mediated NF-kappaB activation, but not TNFalpha mediated NF-kappaB activation ... In addition, our study shows that ATM, but not ATM-Rad3 related (ATR) or DNA dependent protein kinase (DNA-PK) is involved in UV-induced NF-kappaB activation ... In addition, our study shows that ATM, but not ATM-Rad3 related (ATR) or DNA dependent protein kinase (DNA-PK) is involved in UV-induced NF-kappaB activation
Lim et al., Am J Physiol Heart Circ Physiol 2007 (Atherosclerosis) : A subsequent PCB induced increase in NF-kappaB DNA binding may have implications in oxidative stress mediated inflammatory mechanisms
Santoro et al., Nat Genet 2007 : Using genetic and molecular approaches, we show that Birc2 positively regulates the formation of the TNF receptor complex I in endothelial cells, thereby promoting NF-kappaB activation and maintaining vessel integrity and stabilization
Miura et al., J Hepatol 2007 (Liver Cirrhosis) : Epimorphin , a morphogenic protein, induces proteases in rodent hepatocytes through NF-kappaB
Lin et al., Biochim Biophys Acta 2007 : The MyD88 activated nuclear factor-kappaB (NF-kappaB) signaling pathway and the intracellular upregulation of NF-kappaB signaling can induce an antiviral effect ... The strong activation of NF-kappaB by the IkappaB kinase complex IKKalpha/IKKbeta dramatically suppressed HBV replication ; the MyD88 dominant negative mutant that abrogated NF-kappaB activity did not inhibit HBV replication ... The strong activation of NF-kappaB by the IkappaB kinase complex IKKalpha/IKKbeta dramatically suppressed HBV replication ; the MyD88 dominant negative mutant that abrogated NF-kappaB activity did not inhibit HBV replication
Tokuhara et al., Nitric Oxide 2008 : Rebamipide enhanced the degradation of IkappaBalpha and the activation of NF-kappaB ... These findings demonstrate that rebamipide up-regulates iNOS by iNOS promoter activation through NF-kappaB , and by its mRNA stabilization presumably through the super-induction of IL-1RI and antisense-transcript
Kairisalo et al., Biochem Biophys Res Commun 2007 : X-linked inhibitor of apoptosis protein increases mitochondrial antioxidants through NF-kappaB activation ... Studies using reporter constructs and NF-kappaB Rel-A deficient mouse embryonic fibroblasts showed that NF-kappaB signaling is required for the induction of Sod2 by XIAP
Kim et al., J Ethnopharmacol 2007 : Furthermore, macrophages stimulated with LPS generated ROS via activation of membrane bound NADPH oxidase , and ROS played an important role in the activation of nuclear factor-kappaB (NF-kappaB) and MAPKs
Menschikowski et al., Thromb Res 2008 (Inflammation) : In this study we investigated the effects of activated protein C (APC) on sPLA ( 2 ) -IIA expression, phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2, and on DNA binding activities of nuclear factor-kappaB (NF-kappaB) and CCAAT box enhancer binding protein-beta ( C/EBP-beta ) in human aortic smooth muscle cells ( HASMC ) ... At the same time, APC increased the phosphorylation of ERK 1/2 and decreased NF-kappaB and C/EBP-beta DNA binding activities in these cells, as compared with respective stimulated controls
Yang et al., Mol Immunol 2008 (Escherichia coli Infections...) : Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 ( CXCL8 ) expression in the udder ... Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 ( CXCL8 ) expression in the udder ... Bovine TLR2 and TLR4 properly transduce signals from Staphylococcus aureus and E. coli, but S. aureus fails to both activate NF-kappaB in mammary epithelial cells and to quickly induce TNFalpha and interleukin-8 ( CXCL8 ) expression in the udder ... In contrast, E. coli and LPS activate strongly NF-kappaB in these cells ... Yet, preparations of heat killed bacteria from both pathogens activate equally well bovine TLR2 and TLR4 receptors to induce NF-kappaB activation, as shown in the HEK293 reconstitution system of TLR-signal transduction ... Yet, preparations of heat killed bacteria from both pathogens activate equally well bovine TLR2 and TLR4 receptors to induce NF-kappaB activation, as shown in the HEK293 reconstitution system of TLR-signal transduction
Cruz et al., Infect Immun 2008 (Inflammation...) : Our combined results demonstrate that B. burgdorferi confined to phagolysosomes is a potent inducer of cytosolic signals that result in ( i ) production of NF-kappaB dependent cytokines, ( ii ) assembly of the inflammasome and activation of caspase-1, and ( iii ) induction of programmed cell death ... Our combined results demonstrate that B. burgdorferi confined to phagolysosomes is a potent inducer of cytosolic signals that result in ( i ) production of NF-kappaB dependent cytokines, ( ii ) assembly of the inflammasome and activation of caspase-1 , and ( iii ) induction of programmed cell death
Lee et al., Mol Cancer Ther 2007 (Colonic Neoplasms) : In addition, caspase-3 inhibitor reduced epothilone B-induced cell death and NF-kappaB activation
Cheney et al., Cancer Biol Ther 2008 (Rhabdomyosarcoma) : Previously MDM2 has been shown to activate NFkappaB through activation of transcription of the p65RelA subunit ... Expression of MDM2 in RD cells transcriptionally repressed p65RelA and suppressed NFkappaB activity, resulting in a reduced growth rate and enhanced apoptosis
Yang et al., Biochem J 2008 (Breast Neoplasms) : In addition, we found that HRG activates NF-kappaB ( nuclear factor kappaB ) in a Rac1- and MEK dependent fashion, and inhibition of NF-kappaB abrogates cyclin D1/p21(Cip1) induction and proliferation by HRG
Shembade et al., J Virol 2007 : Ubiquitination of the human T-cell leukemia virus 1 Tax oncoprotein provides an important regulatory mechanism that promotes the Tax mediated activation of NF-kappaB ... Tax interacts with Ubc13, and small interfering RNA mediated knockdown of Ubc13 expression abrogates Tax ubiquitination and the activation of NF-kappaB ... Mouse fibroblasts lacking Ubc13 exhibit impaired Tax activation of NF-kappaB despite normal tumor necrosis factor- and interleukin-1 mediated NF-kappaB activation ... Mouse fibroblasts lacking Ubc13 exhibit impaired Tax activation of NF-kappaB despite normal tumor necrosis factor- and interleukin-1 mediated NF-kappaB activation ... Collectively, our results reveal that Ubc13 is essential for Tax ubiquitination, its interaction with NEMO, and Tax mediated NF-kappaB activation ... Collectively, our results reveal that Ubc13 is essential for Tax ubiquitination, its interaction with NEMO, and Tax mediated NF-kappaB activation
Ahn et al., Cancer Res 2007 (Skin Neoplasms) : In agreement with this, we also found that TNF activated NQO2, and NQO2-specific small interfering RNA abrogated the TNF induced NQO2 activity and NF-kappaB activation
von Bismarck et al., Crit Care Med 2007 (Respiratory Distress Syndrome, Newborn) : Inhibition of NF-[kappa]B suppressed acid sphingomyelinase activity and ceramide generation, indicating a novel proinflammatory link of NF-[kappa]B
Lee et al., J Agric Food Chem 2007 : Here we report that 3,3',4',5,5',7-hexahydroxyflavone ( myricetin ), one of the major flavonols in red wine, inhibits 12-O-tetradecanoylphorbol-13-acetate ( phorbol ester ) -induced COX-2 expression in JB6 P+ mouse epidermal ( JB6 P+ ) cells by suppressing activation of nuclear factor kappa B (NF-kappaB) ... In addition, red wine extract inhibited phorbol ester induced COX-2 expression and NF-kappaB transactivation in JB6 P+ cells
Yao et al., Oral Dis 2007 (Tongue Neoplasms) : Furthermore, L-685,458 down-regulated cyclin D1 , B-cell lymphocytic-leukemia proto-oncogene 2 and c-Myc expressions, which are regulated by the transcription factor NF-kappaB ... Furthermore, L-685,458 down-regulated cyclin D1, B-cell lymphocytic-leukemia proto-oncogene 2 and c-Myc expressions, which are regulated by the transcription factor NF-kappaB
Yang et al., J Biol Chem 2007 (Tuberculosis) : We find Rip2 required for Nod2 mediated NF-kappaB activation and to a lesser extent mitogen activated protein kinase activation ... We also demonstrate a requirement for the E2-conjugating enzyme Ubc13, the E3 ubiquitin ligase Traf6 , and the ubiquitin activated kinase Tak1 in Nod2 mediated NF-kappaB activation ... We also demonstrate a requirement for the E2-conjugating enzyme Ubc13 , the E3 ubiquitin ligase Traf6, and the ubiquitin activated kinase Tak1 in Nod2 mediated NF-kappaB activation ... We also demonstrate a requirement for the E2-conjugating enzyme Ubc13, the E3 ubiquitin ligase Traf6, and the ubiquitin activated kinase Tak1 in Nod2 mediated NF-kappaB activation ... We also demonstrate a requirement for the E2-conjugating enzyme Ubc13, the E3 ubiquitin ligase Traf6, and the ubiquitin activated kinase Tak1 in Nod2 mediated NF-kappaB activation
Heidemann et al., Am J Physiol Gastrointest Liver Physiol 2007 : The strongest induction was induced by proinflammatory factors known to utilize the NF-kappaB pathway, and expression of EBI3 and IL-23p19 was diminished by an NF-kappaB inhibitor ... The strongest induction was induced by proinflammatory factors known to utilize the NF-kappaB pathway, and expression of EBI3 and IL-23p19 was diminished by an NF-kappaB inhibitor
Romagnoli et al., Clin Cancer Res 2007 (Multiple Myeloma) : Of particular interest, AS602868 strongly increased myeloma sensitivity to TRAIL in blocking TRAIL induced NF-kappaB activation and in decreasing the expression of antiapoptotic proteins such as cFLIP and cIAP-1/2
Ishizuka et al., J Pharmacol Exp Ther 2008 : Inhibition of NF-kappaB activation and inhibition of ERK1/2 phosphorylation inhibited 20-HETE induced ICAM expression ... Inhibition of NF-kappaB activation and inhibition of ERK1/2 phosphorylation inhibited 20-HETE induced ICAM expression ... It seems that 20-HETE triggers NF-kappaB and MAPK/ERK activation and that both signaling pathways participate in the cellular mechanisms by which 20-HETE activates vascular endothelial cells ... It seems that 20-HETE triggers NF-kappaB and MAPK/ERK activation and that both signaling pathways participate in the cellular mechanisms by which 20-HETE activates vascular endothelial cells
Oh et al., J Immunol 2007 : The activation of NF-kappaB by neutrophil lactoferrin (Lf) is regulated via the IkappaB kinase (IKK) signaling cascade, resulting in the sequential phosphorylation and degradation of IkappaB
Ronchetti et al., J Immunol 2007 : As a consequence, up-regulation of Bcl-x ( L ), one of the major target proteins of CD28 dependent NF-kappaB activation, was defective in costimulated GITR ( -/- ) CD8 ( + ) T cells
Tudhope et al., J Immunol 2007 : The role of IkappaB kinase 2, but not activation of NF-kappaB , in the release of CXCR3 ligands from IFN-gamma stimulated human bronchial epithelial cells
Madhavan et al., J Immunol 2007 : Biomechanical signals suppress TAK1 activation to inhibit NF-kappaB transcriptional activation in fibrochondrocytes
Heo et al., J Immunol 2007 : This leads to NFkappaB activation as a result of IkappaBalpha degradation and/or JNK/AP-1 activation, and ultimately results in the expression of genes required for cell survival, cytokine production, or cell proliferation ... This leads to NFkappaB activation as a result of IkappaBalpha degradation and/or JNK/AP-1 activation, and ultimately results in the expression of genes required for cell survival, cytokine production, or cell proliferation
Hill et al., Eur J Immunol 2007 : Concerning the molecular mechanisms of this effect, IDO acts as an intermediate pathway in LPS induced production of reactive oxygen species and NF-kappaB activation, two processes that lead to DC maturation
Voboril et al., Neoplasma 2007 (Colorectal Neoplasms) : IL-4 or IL-10 ( 1, 10, 100 ng/ml ) neither inhibited growth and clonogenicity nor activated NF-kappaB , but they sensitized cells to irradiation in a dose dependent manner ... IL-4 or IL-10 ( 1, 10, 100 ng/ml ) neither inhibited growth and clonogenicity nor activated NF-kappaB , but they sensitized cells to irradiation in a dose dependent manner
Lu et al., Mol Immunol 2008 : It thus activates downstream signaling events to activate transcription factor NF-kappaB , and induce the expression of IL-1 responsive genes such as TNF-alpha and GM-CSF ... It thus activates downstream signaling events to activate transcription factor NF-kappaB , and induce the expression of IL-1 responsive genes such as TNF-alpha and GM-CSF ... It thus activates downstream signaling events to activate transcription factor NF-kappaB , and induce the expression of IL-1 responsive genes such as TNF-alpha and GM-CSF
Quinones et al., Mol Cell Neurosci 2008 (Demyelinating Diseases...) : Using an in vitro system we demonstrate that expression of CCR2 was functional in astrocytes and that signaling via this receptor lead to activation of NF-kB and Akt and was associated with increased astrocyte survival
Hyun Hwa Cho et al., Biochim Biophys Acta 2008 : Downregulation of lzts2 increased the proliferation of hASCs and hBMSC, and blocked the NF-kappaB inhibitor induced inhibitory effect on their proliferation and Tcf promoter activation
Wiesner et al., Oncogene 2008 : Alpha-catulin , a Rho signalling component, can regulate NF-kappaB through binding to IKK-beta, and confers resistance to apoptosis ... Alpha-catulin, a Rho signalling component, can regulate NF-kappaB through binding to IKK-beta , and confers resistance to apoptosis
Bai et al., Scand J Immunol 2007 (Colitis...) : The mice with colitis treated by AN showed less tissue damage, less MPO activity, less TNF-alpha production in colon, and inhibited NF-kappaB activation in LPMC, compared with those mice with colitis untreated, whereas the mice with colitis treated by CHD showed the worst tissue damage, the highest MPO activity, the highest TNF-alpha level, and enlarged NF-kappaB activation in LPMC
Gao et al., Am J Hypertens 2007 (Inflammation) : These results indicate that MCP-1 expression in inflammatory vascular injury is regulated by activation of NF-kappaB
Lohmann et al., J Endotoxin Res 2007 : Our results indicate that E. colic LPS activated NF-kappaB in cells transfected with all combinations of the three receptor proteins, whereas RsLPS activated NF-kappaB only in cells expressing the single combination of equine TLR4 and equine MD-2
Li et al., Nan Fang Yi Ke Da Xue Xue Bao 2007 (Burns...) : Compared to that of the control group, activity of pulmonary NF-kappaB in burned rats was markedly increased within 1 PBH and kept increasing till 24 h. Expressions of pulmonary TNF alpha and IL-8 mRNAs increased gradually, reaching the peak level at 6 PBH, and PDTC could effectively inhibit pulmonary NF-kappaB activation and expression of the pulmonary cytokines induced by the burn injury ... Compared to that of the control group, activity of pulmonary NF-kappaB in burned rats was markedly increased within 1 PBH and kept increasing till 24 h. Expressions of pulmonary TNF alpha and IL-8 mRNAs increased gradually, reaching the peak level at 6 PBH, and PDTC could effectively inhibit pulmonary NF-kappaB activation and expression of the pulmonary cytokines induced by the burn injury
Guan et al., J Virol 2008 : In this study, we demonstrate that the phosphorylation of p50 and p65 by the catalytic subunit of protein kinase A ( PKAc ) is essential for NF-kappaB DNA binding and transactivation activity
Amir-Zilberstein et al., J Biol Chem 2008 : Upon NF-kappaB activation, inhibition of the A20 gene by DSIF persists, but now NF-kappaB and the core promoter regulate DSIF instead of ELIE
Lu et al., Virology 2008 (Carcinoma, Hepatocellular...) : We also showed that transcription factor NF-kappaB is required for the activation of COX-2 regulated by NS3
Xiao et al., Immunol Lett 2007 : Gel shift and reporter gene analyses revealed linomide inhibited LPS induced NF-kappaB binding to the NF-kappaB consensus oligonucleotide and NF-kappaB mediated reporter gene expression ... Taken together, these results suggest that linomide inhibits TNF-alpha production by suppressing the activation of NF-kappaB and mitogen activated protein kinase ( MAPK ), which might, at least in part, contribute to the beneficial effects of linomide in the treatment of autoimmune diseases
Kim et al., J Biol Chem 2008 : We found that TAK1 deletion completely abolished MDP-NOD2 signaling, activation of NF-kappaB and MAPKs, and subsequent induction of cytokines/chemokines in keratinocytes
Chen et al., Br J Pharmacol 2008 : Curcumin inhibits connective tissue growth factor gene expression in activated hepatic stellate cells in vitro by blocking NF-kappaB and ERK signalling ... Activation of NF-kappaB by dominant active IkappaB kinase (IKK) , or inhibition of NF-kappaB by dominant negative IkappaBalpha, caused the stimulation, or suppression of the ctgf promoter activity, respectively
Chun et al., Evidence-based complementary and alternative medicine : eCAM 2007 : In these concentrations, MCE inhibited the production of NO and prostaglandin E ( 2 ) ( PGE ( 2 ) ), the expression of inducible NO synthase (iNOS) , cyclooxygenase-2 (COX-2) and phosphorylated inhibitor of kappa Bal pha ( p-I kappa Bal pha ), and the activation of nuclear factor kappa B (NF-kappa B) ... In these concentrations, MCE inhibited the production of NO and prostaglandin E ( 2 ) ( PGE ( 2 ) ), the expression of inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2) and phosphorylated inhibitor of kappa Bal pha ( p-I kappa Bal pha ), and the activation of nuclear factor kappa B (NF-kappa B) ... These results demonstrate that MCE has anti-inflammatory effects through the inhibition of iNOS and COX-2 expression by suppressing the phosphorylation of I-kappa Bal pha and the activation of NF-kappa B
Porter et al., J Toxicol Environ Health 2007 : Molecular studies confirmed that LPS stimulated NF-kappaB mRNA expression, and PGJ2 reduced this stimulation, which is consistent with PGJ2 effect on LPS induced production of NO, TNF-alpha and MIP-2
Shultz et al., J Interferon Cytokine Res 2007 : Activation of NF-kappaB requires the inhibitor of kappaB (IkappaB) kinase ( IKK) complex, which is made up of two functional kinases, IKKalpha and IKKbeta, and the scaffolding protein IKKgamma ... Activation of NF-kappaB requires the inhibitor of kappaB (IkappaB) kinase ( IKK) complex , which is made up of two functional kinases, IKKalpha and IKKbeta, and the scaffolding protein IKKgamma ... Although the IFN-gamma induced expression of the IKKbeta dependent gene ip-10 is sensitive to the superrepressor ( SR ) of IkappaBalpha and requires the p65 subunit of NF-kappaB, IFN-gamma does not activate NF-kappaB ... In summary, a distinct subset of IFN-gamma dependent genes requires some components of the normal pathway of NF-kappaB activation without activation of NF-kappaB signaling by IFN-gamma
Kim et al., Biogerontology 2008 : The activation of NF-kappaB through Akt induced FOXO1 phosphorylation during aging and its modulation by calorie restriction ... These results indicate that insulin promoted NF-kappaB activation through phosphorylation of FOXO1 by upregulating PI3K/Akt signaling
Ko et al., Mol Endocrinol 2008 (Prostatic Neoplasms) : In contrast, androgen independent C4-2 ( LNCaP derived ) cells fail to show AR down-regulation by TNFalpha, despite expression of B-myb and TNFalpha induced NF-kappaB activity similar to that in LNCaP cells ... In C4-2 cells, neither NF-kappaB and B-myb, nor any of the corepressor components, were detected at the negative site in response to TNFalpha
Dey et al., Cell Death Differ 2008 : We show in this study that R-Roscovitine can downregulate nuclear factor-kappa B (NF-kappaB) activation in response to tumor necrosis factor (TNF)alpha and interleukin 1 ... We show in this study that R-Roscovitine can downregulate nuclear factor-kappa B (NF-kappaB) activation in response to tumor necrosis factor (TNF)alpha and interleukin 1
Kalariya et al., Exp Eye Res 2008 : Furthermore, CDA induced the activation of NF-kappaB and AP-1 which was significantly inhibited by NAC
Lee et al., Int Immunol 2007 : Hydrogen peroxide increases human leukocyte adhesion to porcine aortic endothelial cells via NFkappaB dependent up-regulation of VCAM-1 ... In summary, our results suggest that NFkappaB dependent porcine VCAM-1 expression by H ( 2 ) O ( 2 ) may promote interaction of human leukocyte to PAECs, and thus may play an important role on inducing xenogeneic immune responses
Wright et al., Dev Cell 2007 : Interestingly, the loss of CYLD in testicular cells leads to activation of the transcription factor NF-kappaB and aberrant expression of antiapoptotic genes ... We further show that CYLD negatively regulates a ubiquitin dependent NF-kappaB activator, RIP1 ... We further show that CYLD negatively regulates a ubiquitin dependent NF-kappaB activator, RIP1
Huang et al., J Immunol 2007 : Requirement for both JAK mediated PI3K signaling and ACT1/TRAF6/TAK1 dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells ... Requirement for both JAK mediated PI3K signaling and ACT1/TRAF6/TAK1 dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells ... Requirement for both JAK mediated PI3K signaling and ACT1/TRAF6/TAK1 dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells ... Requirement for both JAK mediated PI3K signaling and ACT1/TRAF6/TAK1 dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells ... Requirement for both JAK mediated PI3K signaling and ACT1/TRAF6/TAK1 dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells
Qin et al., J Immunol 2007 : Two-week-old TRAF6-deficient mice showed severe defects in B cell follicle and marginal zone formation, similar to mutant mice defective in lymphotoxin (Lt) beta receptor ( LtbetaR ) signal induction of nonclassical NF-kappaB activation
Kim et al., J Immunol 2007 (Asthma...) : Our results also showed that PI3K inhibitors or AdPTEN down-regulated a transcription factor, NF-kappaB activity, and BAY 11-7085 substantially reduced the increased levels of IL-17 after TDI inhalation
Funami et al., J Immunol 2007 : The receptor interacting protein 1 (RIP1), a crucial signaling molecule for TICAM-1 mediated NF-kappaB activation, accumulated in the TICAM-1 speckles
Lee et al., J Immunol 2007 (Respiratory Distress Syndrome, Adult) : PP1 also blocked LPS induced NF-kappaB activation, and phosphorylation and degradation of IkappaB-alpha ... PP1 also blocked LPS induced NF-kappaB activation, and phosphorylation and degradation of IkappaB-alpha ... Moreover, treatment with anti-alpha ( v ), anti-beta ( 3 ), or Arg-Gly-Asp-Ser ( RGDS ), inhibited LPS induced NF-kappaB activation ... Taken together, our findings suggest that Src TKs play a critical role in LPS induced activations of NF-kappaB and integrin ( alpha ( v ) beta ( 3 ) ) signaling during acute lung injury
Tsuruta et al., J Immunol 2007 (Edema...) : Vitronectin ( -/- ) neutrophils consistently produced more TNF-alpha, MIP-2, and IL-1beta after LPS exposure than did vitronectin ( +/+ ) neutrophils and also showed greater degradation of IkappaB-alpha and increased LPS induced nuclear accumulation of NF-kappaB compared with vitronectin ( +/+ ) neutrophils
Saha et al., J Immunol 2007 (Inflammation) : MAPK p38 regulates transcriptional activity of NF-kappaB in primary human astrocytes via acetylation of p65 ... Several studies have shown that blocking p38 activity attenuates the transcriptional activity of the proinflammatory transcription factor NF-kappaB without altering its DNA binding activity ... However, the molecular mechanism of p38 mediated regulation of NF-kappaB remains largely unknown ... In this study, we delineate that p38 controls the transcriptional activity of NF-kappaB by regulating acetylation of p65, but not its phosphorylation
Jang et al., Int J Oncol 2007 : NF-kappaB inhibitors significantly attenuated the expression of B7-DC , -H1, -H2, and -H3 mRNA in response to TPA
Jomphe et al., Basic Clin Pharmacol Toxicol 2008 : Chondroitin sulfate reduced IL-1beta induced NF-kappaB nuclear translocation, but not AP-1 translocation, it decreased IL-1beta induced phosphorylation of Erk1/2 and abrogated p38MAPK phosphorylation, but did not prevent IL-1beta induced increase in nitrite
Petersson et al., BMC cancer 2007 (Breast Neoplasms) : We showed that IFN-gamma treatment and the inhibition of the transcription factor NFkappaB had a synergistic effect on psoriasin levels ... We showed that IFN-gamma treatment and the inhibition of the transcription factor NFkappaB had a synergistic effect on psoriasin levels
Yu et al., Biochem Biophys Res Commun 2008 : We found that TAB2 physically interacts with Tax and augments Tax induced NF-kappaB activity ... We found that TAB2 physically interacts with Tax and augments Tax induced NF-kappaB activity
Kanetaka et al., Exp Cell Res 2008 : Interferon-alpha induces transient upregulation of c-FLIP through NF-kappaB activation ... Together, these results demonstrate that IFN-alpha causes a transient upregulation of c-FLIP expression, at least through PKCalpha mediated activation of NF-kappaB
Zhang et al., Neuroscience 2007 : NFkappa-B (NFkappa B) , a key regulator of COX-2 expression, was activated 72 h after MA administration, and was accompanied by increased Ikappa B ( Ikappa B ) phosphorylation
Yu et al., Biomed Pharmacother 2008 (Carcinoma, Hepatocellular...) : NF-kappa B inhibitor induced a marked reduction in MMP-9 expression, and it suggested that NF-kappa B could play an important role in TNF-alpha mediated MMP-9 expression ... Furthermore, resveratrol significantly suppressed TNF-alpha mediated NF-kappa B expression and invasion of HepG2 cells
Diessenbacher et al., J Invest Dermatol 2008 : Although tumor necrosis factor (TNF) related apoptosis inducing ligand ( TRAIL ) and TNF both activate NF-kappaB in human keratinocytes, only TRAIL potently induces apoptosis ... Although tumor necrosis factor (TNF) related apoptosis inducing ligand ( TRAIL ) and TNF both activate NF-kappaB in human keratinocytes, only TRAIL potently induces apoptosis ... However, when induction of NF-kappaB was inhibited with a kinase dead IKK2 mutant ( IKK2-KD ), TNF- but not TRAIL induced apoptosis was dramatically enhanced
Cepinskas et al., Am J Physiol Gastrointest Liver Physiol 2008 (Disease Models, Animal...) : Induction of CLP resulted in increased PMN accumulation, ICAM-1 expression, and activation of NF-kappaB in the liver of septic mice ... Induction of CLP resulted in increased PMN accumulation, ICAM-1 expression, and activation of NF-kappaB in the liver of septic mice ... Induction of CLP resulted in increased PMN accumulation, ICAM-1 expression, and activation of NF-kappaB in the liver of septic mice ... In in vitro experiments, CORM-2 released CO attenuated LPS induced production of ROS and NO, activation of NF-kappaB , increase in ICAM-1 and iNOS protein expression and PMN adhesion to LPS stimulated HUVEC ... In in vitro experiments, CORM-2 released CO attenuated LPS induced production of ROS and NO, activation of NF-kappaB , increase in ICAM-1 and iNOS protein expression and PMN adhesion to LPS stimulated HUVEC
Bhoopathi et al., J Biol Chem 2008 (MAP Kinase Signaling System...) : ERK inhibition, using a kinase-dead mutant for ERK, ameliorated NF-kappaB activation and caspase mediated apoptosis in Ad-MMP-9 infected cells
Zerfaoui et al., Cell Signal 2008 : Nuclear translocation of p65 NF-kappaB is sufficient for VCAM-1 , but not ICAM-1, expression in TNF stimulated smooth muscle cells : Differential requirement for PARP-1 expression and interaction ... Nuclear translocation of p65 NF-kappaB is sufficient for VCAM-1, but not ICAM-1 , expression in TNF stimulated smooth muscle cells : Differential requirement for PARP-1 expression and interaction ... Here we show that while p65 NF-kappaB nuclear translocation in TNF treated smooth muscle cells ( SMCs ) was sufficient for the expression of VCAM-1 , expression of ICAM-1 showed a critical requirement for PARP-1
Saja et al., Int Immunopharmacol 2007 : Immunoblot analysis suggested that ALA induced activation of NFkappaB leading to the upregulation of MMP-9 was due to the degradation of IkappaB-alpha
Huh et al., Int Immunopharmacol 2007 (MAP Kinase Signaling System) : Inhibition of iNOS protein expression appears to be at the transcriptional level, since prodigiosin decreased LPS induced NF-kappaB activity through preventing the degradation of IkBalpha, with significant inhibition achieved following pretreatment with prodigiosin
Kim et al., Brain Res 2007 : The transcriptional activity of NF-kappaB was significantly increased by GSK-3beta inhibition, which was determined by nuclear translocation of NF-kappaB ... Inhibition of ERK1/2 activity by U-0126 also completely blocked the nuclear translocation of NF-kappaB
Brunelleschi et al., Pharmacol Res 2007 : In a concentration dependent manner, MPC-OOE inhibited p50 and p65 NF-kappaB translocation in both un-stimulated and phorbol-myristate acetate ( PMA ) -challenged cells, being particularly effective on the p50 subunit
Zhang et al., Arthritis Res Ther 2007 (Arthritis, Experimental...) : TNF stimulated VEGF-C expression by OCPs and increased nuclear factor-kappa B (NF-kappaB) binding to an NF-kappaB sequence in the VEGF-C promoter
Takamune et al., Biochem Biophys Res Commun 2008 (Carcinoma, Squamous Cell...) : On the other hand, TNFalpha activated a transcription factor NF-kappaB in OSCC cells, while NBD peptide, an NF-kappaB inhibitor, inhibited the ADAM-17 maturation, thus suggesting that NF-kappaB is involved in ADAM-17 maturation
Bachmeier et al., Carcinogenesis 2008 (Breast Neoplasms) : Using small interfering RNA techniques, we elucidated the underlying molecular mechanism revealing that reduction of CXCL1 and -2 messenger RNA levels is NFkappaB dependent and requires intact IkappaBalpha expression
Secchiero et al., Blood 2008 : OPG promoter functional assays and chromatin immunoprecipitation experiments revealed inhibitory effects of Nutlin-3 on the TNF-alpha induced NF-kappaB DNA binding activity to the OPG promoter
Hayashi et al., Proc Natl Acad Sci U S A 2007 (Asthma...) : Here we report that HAA inhibits NF-kappaB activation upon T cell antigen receptor engagement by specifically targeting PDK1
Kim et al., Lung Cancer 2008 (Lung Neoplasms) : Transfection studies with siRNA specific for Nrf2 and NF-kappaB revealed that HO-1 expression in A549 cells is mediated by transcriptional activation of Nrf2, but not NF-kappaB
Funderburg et al., Proc Natl Acad Sci U S A 2007 : In studies with HEK cells engineered to express various TLRs, we show that activation of NF-kappaB by hBD-3 depends on the expression of both TLR1 and TLR2
Sur et al., J Invest Dermatol 2008 : Hsp27 regulates pro-inflammatory mediator release in keratinocytes by modulating NF-kappaB signaling ... Further studies showed that downregulation of Hsp27 resulted in induction of NF-kappaB reporter activity in keratinocytes ... As synthesis of the pro-inflammatory cytokine IL-8 and the prostanoid PGE ( 2 ) are regulated by NF-kappaB , this could be a probable mechanism by which Hsp27 modulates the production of these inflammatory cytokines
Lee et al., Prostate 2008 (Prostatic Neoplasms) : Previous studies demonstrated that IL-4 enhances androgen receptor (AR) activation mediated by NF-kappaB in the absence or in the very low levels of androgen in prostate cancer cells
Massoumi et al., Bioessays 2007 (Carcinoma, Adenoid Cystic) : Focusing on recent data concerning the normal functions and signaling interactions of the CYLD gene product, we explain how CYLD interferes with TNF-alpha or TLR mediated signaling as well as with JNK or NF-kappaB dependent p65/50 signaling to limit inflammation
Varfolomeev et al., Cell 2007 (Neoplasms) : IAP antagonists induce autoubiquitination of c-IAPs, NF-kappaB activation , and TNFalpha dependent apoptosis
Vince et al., Cell 2007 : In sensitive tumor lines, IAP antagonist induced NF-kappaB stimulated production of TNFalpha that killed cells in an autocrine fashion ... Inhibition of NF-kappaB reduced TNFalpha production, and blocking NF-kappaB activation or TNFalpha allowed tumor cells to survive IAC induced apoptosis
Kim et al., Blood 2008 (Lymphoma, Large B-Cell, Diffuse) : In addition, we show that NF-kappaB activation induced by B cell activating factor of tumor necrosis factor family ( BAFF ) in DLBCL cells requires PKK
El Kasmi et al., J Immunol 2007 : ETV3 and SBNO2 repressed NF-kappaB- but not IFN regulatory factor 7 (IRF7) activated transcriptional reporters ... ETV3 and SBNO2 repressed NF-kappaB- but not IFN regulatory factor 7 (IRF7) activated transcriptional reporters
Rui et al., J Immunol 2007 : In this study, we demonstrate that PECAM-1 ligation with CD38-Fc fusion protein negatively regulates LPS induced proinflammatory cytokine TNF-alpha , IL-6, and IFN-beta production by inhibiting JNK, NF-kappaB , and IFN regulatory factor 3 activation in macrophages ... In this study, we demonstrate that PECAM-1 ligation with CD38-Fc fusion protein negatively regulates LPS induced proinflammatory cytokine TNF-alpha, IL-6, and IFN-beta production by inhibiting JNK, NF-kappaB , and IFN regulatory factor 3 activation in macrophages ... In this study, we demonstrate that PECAM-1 ligation with CD38-Fc fusion protein negatively regulates LPS induced proinflammatory cytokine TNF-alpha, IL-6 , and IFN-beta production by inhibiting JNK, NF-kappaB , and IFN regulatory factor 3 activation in macrophages ... In this study, we demonstrate that PECAM-1 ligation with CD38-Fc fusion protein negatively regulates LPS induced proinflammatory cytokine TNF-alpha, IL-6, and IFN-beta production by inhibiting JNK, NF-kappaB , and IFN regulatory factor 3 activation in macrophages
Tucker et al., J Immunol 2007 : These findings demonstrate a key role for NF-kappaB2 in the regulation of RelA activation and suggest overlap in the function of NF-kappaB members in canonical and noncanonical pathway signaling
Bartels et al., J Immunol 2007 (Helicobacter Infections) : Peptide mediated disruption of NFkappaB/NRF interaction inhibits IL-8 gene activation by IL-1 or Helicobacter pylori ... Previous studies suggested that interaction of transcription factors NFkappaB repressing factor (NRF) and NFkappaB play a crucial role in activation of IL-8 gene expression
Zhou et al., J Immunol 2007 (Bacteroidaceae Infections) : Using gene knockout mice and siRNA to silence mouse genes, we showed that both TLR2 and TLR7 are essential for the induction of NFkappaB containing genes and NFkappaB-IFN-sensitive response element ( ISRE ) cocontaining genes by either P. gingivalis or its purified components ... Using gene knockout mice and siRNA to silence mouse genes, we showed that both TLR2 and TLR7 are essential for the induction of NFkappaB containing genes and NFkappaB-IFN-sensitive response element ( ISRE ) cocontaining genes by either P. gingivalis or its purified components
Seldon et al., J Immunol 2007 (Inflammation) : HO-1 inhibits NF-kappaB ( i.e., RelA ) phosphorylation at Ser ( 276 ), a phosphoacceptor that is critical to sustain TNF-driven NF-kappaB activity in EC
Davé et al., J Immunol 2007 (Chronic Disease...) : In a NF-kappaB reporter system, 8K-NBD dose-dependently inhibits TNF induced NF-kappaB activation
Azim et al., J Immunol 2007 : We tested the hypothesis that NF-kappaB inducing kinase (NIK) regulates COX-2 gene expression in macrophages through inducible phosphorylation of PU.1
González-Ramos et al., Gynecol Obstet Invest 2008 (Disease Models, Animal...) : This study demonstrates, for the first time, that the NF-kappaB pathway is implicated in the development of endometriotic lesions in vivo and that NF-kappaB inhibition reduces ICAM-1 expression and cell proliferation, but increases apoptosis of endometriotic lesions, diminishing the initial development of endometriosis in an animal model
Song et al., Immunology 2008 (Translocation, Genetic) : Redox factor-1 mediates NF-kappaB nuclear translocation for LPS induced iNOS expression in murine macrophage cell line RAW 264.7
Li et al., Arterioscler Thromb Vasc Biol 2007 (Inflammation) : Reporter gene assays showed that FXR ligands activated an FXR reporter gene and suppressed IL-1beta induced nuclear factor (NF)-kappaB activation and iNOS in a manner that required functional FXR and SHP
Kersse et al., Biochem Soc Trans 2007 (Inflammation) : Furthermore, we discuss the functional characteristics of the caspase-1 related CARD-only proteins in relation to caspase-1 mediated IL-1beta maturation and NF-kappaB activation
He et al., Acta Pharmacol Sin 2007 (Reperfusion Injury) : We established the rabbit lung transplantation model and studied lung injury induced by ischemia/reperfusion and the inhibitory effect of TRI on NF-kappaB ... TRI blocked NF-kappaB activation and ICAM-1 expression ... The effects of TRI on reducing injury to donor lungs induced by ischemia/reperfusion may possibly be mediated by inhibiting the activity of NF-kappaB and the expression of the NF-kappaB target gene ICAM-1
Shang et al., Am J Physiol Cell Physiol 2008 : Gel shift and chromosomal immunoprecipitation assays confirmed that nuclear factor (NF)-kappaB bound to the scn5a promoter in response to ANG II and H ( 2 ) O ( 2 )
Lee et al., Arterioscler Thromb Vasc Biol 2008 : Furthermore, kappaB-Ras1 ( IkappaB interacting Ras-like protein-1 ) which is known to block IkappaB degradation was found increased, and intranuclear translocation of NF-kappaB was inhibited
Teruya et al., BMC microbiology 2007 : Transfection of dominant negative mutants of NF-kappaB inducing kinase, IkappaB kinase and IkappaB inhibited L. pneumophila mediated activation of IL-8 promoter ... Collectively, these results suggest that L. pneumophila induces activation of NF-kappaB through an intracellular signaling pathway that involves NF-kappaB inducing kinase and IkappaB kinase , leading to IL-8 gene transcription, and that hsp90 acts as a crucial regulator in L. pneumophila induced IL-8 expression, presumably contributing to immune response in L. pneumophila
Kwon et al., Biochem Biophys Res Commun 2008 : In support of this, compared with wild type Akt1, the dominant negative Akt1 mutant was decreased in its nuclear expression, reducing the Ki-Ras induced NF-kappaB transcriptional activation ... Moreover, inhibitors of Ras ( sulindac sulfide and farnesyltransferase inhibitor I ) or PI3K/Akt ( wortmannin ), reduced the amounts of Akt1 and Ki-Ras in the nucleus as well as partial NF-kappaB activity ... Moreover, inhibitors of Ras ( sulindac sulfide and farnesyltransferase inhibitor I ) or PI3K/Akt ( wortmannin ), reduced the amounts of Akt1 and Ki-Ras in the nucleus as well as partial NF-kappaB activity ... The complete inhibition of Ki-Ras induced NF-kappaB activation, however, could only be obtained by combined treatment with wortmannin and proteasome inhibitor-1 ... Accordingly, clonogenic assay showed Akt1 contribution to IkappaBalpha mediated NF-kappaB activation for oncogenic cell growth by Ki-Ras
Jones et al., Journal of inflammation (London, England) 2007 : NF-kappaB activation upregulates inducible nitric oxide synthase leading to enhanced nitric oxide production during an inflammatory response ... Here, we examine the role of protein kinase C isoforms and janus activated kinase 2 (JAK2) activation in NF-kappaB activation and LPS stimulated NO production ... The results further define the role of NF-kappaB in LPS stimulated NO production in RAW macrophages ... The data support a function for PKCepsilon, JAK2 and p38 MAPK in NF-kappaB activation following p65 nuclear import
Ahn et al., Biochem Pharmacol 2008 : Therefore, we evaluated the effect six different statins on TNF induced NF-kappaB activation in human myeloid leukemia cells ... Of the six statins evaluated, only the natural statins ( simvastatin, mevastatin, lovastatin, and pravastatin ), not the synthetic statins ( fluvastatin and atorvastatin ), inhibited TNF induced NF-kappaB activation
Tranter et al., Free Radic Biol Med 2008 (Inflammation) : Furthermore, HO-1 and CO suppress NF-kappaB activation by IL-18 ... Furthermore, HO-1 and CO suppress NF-kappaB activation by IL-18 ... This suppression of NF-kappaB reduces levels of PTEN which relieves IL-18 mediated suppression of Akt activity
Aarenstrup et al., J Invest Dermatol 2008 : In this article we further investigate the molecular mechanisms dictating the NF-kappaB dependent repression of PPARdelta in human keratinocytes
Hsieh et al., Clinical and molecular allergy : CMA 2007 : BAI inhibited NF-kappaB activation in IL-1beta- and TNF-alpha activated HMC-1
Motadi et al., Int Immunopharmacol 2007 (Carcinoma...) : Of the several molecular targets, those receiving attention are p53 gene replacement, Bcl-2 downregulation, apoptosis by induced by TNF, the FAS/CD95 receptor system and TRAIL, and inhibition of NF-kappaB
Sareila et al., J Pharmacol Exp Ther 2008 : Orazipone extensively prevented LPS induced activation of nuclear factor kappaB (NF-kappaB) and signal transducer and activator of transcription ( STAT ) 1, which are important transcription factors for iNOS
Chen et al., Cell Res 2007 : Here we show that p28(GANK) inhibits NF-kappaB activity ... These results suggest that overexpression of p28(GANK) prevents the nuclear localization and inhibits the activity of NF-kappaB/RelA
Saha et al., J Neuroimmune Pharmacol 2006 : Activation of NF-kappaB by TNF-alpha and inhibition of TNF-alpha induced BDNF expression by Deltap65 ( a dominant negative mutant ) and NEMO binding domain peptide ( an inhibitor of NF-kappaB ) suggests that TNF-alpha induces BDNF expression through the activation of NF-kappaB ... Activation of NF-kappaB by TNF-alpha and inhibition of TNF-alpha induced BDNF expression by Deltap65 ( a dominant negative mutant ) and NEMO binding domain peptide ( an inhibitor of NF-kappaB ) suggests that TNF-alpha induces BDNF expression through the activation of NF-kappaB
Sriram et al., J Neuroimmune Pharmacol 2007 (Brain Injuries) : Among the most prominent are the regulation of TNFalpha induced NF-kappaB activation by adapter proteins such as TRADD and TRAF, and second, the heterogeneity of microglia and their distribution pattern across brain regions
Tjalkens et al., J Neurosci Res 2008 : Because NF-kappaB dependent expression of inducible nitric oxide synthase ( NOS2 ) can be antagonized by the nuclear orphan receptor peroxisome proliferator activated receptor-gamma ( PPARgamma ), we postulated that a novel agonist of this receptor, 1,1-bis ( 3'-indolyl ) -1- ( p-trifluoromethylphenyl ) methane ( cDIM1 ), would suppress expression of NOS2 in astrocytes and protect cocultured neuronal cells from apoptosis
Tiemessen et al., Proc Natl Acad Sci U S A 2007 : In addition, the monocytes/macrophages have reduced expression of HLA-DR and a strongly reduced capacity to respond to LPS in terms of proinflammatory mediator production ( IL-1beta , IL-6, IL-8, MIP-1alpha, TNF-alpha ), NFkappaB activation , and tyrosine phosphorylation
Wan et al., Cell 2007 : RPS3 knockdown impaired NF-kappaB mediated transcription of selected p65 target genes but not nuclear shuttling or global protein translation
Yeruva et al., Int J Colorectal Dis 2008 (Adenocarcinoma...) : NF-kappaB dependent synergistic regulation of CXCL10 gene expression by IL-1beta and IFN-gamma in human intestinal epithelial cell lines ... Real-time PCR and ELISA experiments using a specific NF-kappaB inhibitor and transfection experiments with a NF-kappaB binding defective CXCL10 promoter construct revealed that the induction of CXCL10 by IL-1beta and its synergism with IFN-gamma is NF-kappaB dependent
Thompson et al., J Biol Chem 2008 (Inflammation) : LTC ( 4 ) stimulation induced NF-kappaB and AP-1 DNA binding, which involved the formation of a p50/p65 and a c-JUN.c-FOS complex, respectively ... LTC ( 4 ) stimulation induced NF-kappaB and AP-1 DNA binding, which involved the formation of a p50/p65 and a c-JUN.c-FOS complex, respectively
Chakrabarti et al., J Cardiovasc Pharmacol 2007 : Oxidized dipyridamole had no effect on TNFalpha mediated NF kappa B activation
Bruno et al., Cell Death Differ 2008 : This effect is mediated by Che-1 phosphorylation and requires NF-kappaB
Chockalingam et al., Arthritis Rheum 2007 (Osteoarthritis) : This study was undertaken to examine the role of PKCzeta in interleukin-1beta (IL-1beta) induced NF-kappaB signaling in human OA chondrocytes, and ultimately to better understand its function in the regulation of downstream mediators of cartilage matrix degradation ... Phosphorylation of PKCzeta and NF-kappaB was induced by IL-1beta treatment in a time dependent manner, and was specifically inhibited by inhibitors of atypical PKCs. Inhibition of PKCzeta suppressed IL-1beta induced up-regulation of ADAMTS-4 messenger RNA ( mRNA ) and aggrecanase activity ... Our results show that PKCzeta is involved in the regulation of IL-1beta induced NF-kappaB signaling in human OA chondrocytes, which in turn regulates downstream expression of ADAMTS-4 and NOS2
Choi et al., J Microbiol Biotechnol 2007 : In addition, streptochlorin inhibited TNF-alpha induced NF-kappaB activation in the newly developed cell based reporter gene assay
Yang et al., J Cell Mol Med 2008 (Shock, Septic) : In mononuclear phagocytes, C-K significantly repressed the activation of TLR4/lipopolysaccharide (LPS) induced NF-kappaB and mitogen activated protein kinases ( MAPKs ), as well as the secretion of pro-inflammatory cytokines ... In mononuclear phagocytes, C-K significantly repressed the activation of TLR4/lipopolysaccharide (LPS) induced NF-kappaB and mitogen activated protein kinases ( MAPKs ), as well as the secretion of pro-inflammatory cytokines
Baugé et al., J Cell Mol Med 2008 : NFkappaB mediates IL-1beta induced down-regulation of TbetaRII through the modulation of Sp3 expression
Kang et al., Biochem Pharmacol 2008 : SK-126 enhanced ERK1/2 and p38 activation following LPS stimulation, but it did not induce phosphorylation of SAPK/JNK and NF-kappaB ... SK-126 enhanced ERK1/2 and p38 activation following LPS stimulation, but it did not induce phosphorylation of SAPK/JNK and NF-kappaB
Ichiki et al., Am J Physiol Heart Circ Physiol 2008 (Atherosclerosis) : EMSA revealed that CT-1 enhanced NF-kappaB DNA binding activity
Andresen et al., J Immunol 2007 : NF-kappaB activity can be induced by HDAC treatment
Lakshmanan et al., J Immunol 2007 : Caspase-4 interacts with TNF receptor associated factor 6 and mediates lipopolysaccharide induced NF-kappaB dependent production of IL-8 and CC chemokine ligand 4 ( macrophage-inflammatory protein-1 ) ... Caspase-4 interacts with TNF receptor associated factor 6 and mediates lipopolysaccharide induced NF-kappaB dependent production of IL-8 and CC chemokine ligand 4 ( macrophage-inflammatory protein-1 ) ... Because a specific NF-kappaB inhibitor blocked LPS induced IL-8 and CCL4 mRNA expression as well as IL-8 and CCL4 secretion in THP1 cells, we investigated the role of caspase-4 in NF-kappaB signaling ... Because a specific NF-kappaB inhibitor blocked LPS induced IL-8 and CCL4 mRNA expression as well as IL-8 and CCL4 secretion in THP1 cells, we investigated the role of caspase-4 in NF-kappaB signaling ... Because a specific NF-kappaB inhibitor blocked LPS induced IL-8 and CCL4 mRNA expression as well as IL-8 and CCL4 secretion in THP1 cells, we investigated the role of caspase-4 in NF-kappaB signaling ... LPS induced NF-kappaB nuclear translocation and activation were inhibited in all caspase-4-deficient clones
Fontalba et al., J Immunol 2007 : NLRP2 , an inhibitor of the NF-kappaB pathway, is transcriptionally activated by NF-kappaB and exhibits a nonfunctional allelic variant ... Notably, stimulation of HEK293T cells with TNF-alpha or overexpression of the p65 subunit of NF-kappaB resulted in up-regulation of NLRP2 and the formation of NF-kappaB-NLRP2 promoter complexes ... Thus, NLRP2 may control NF-kappaB activation through a regulatory loop ... Thus, NLRP2 may control NF-kappaB activation through a regulatory loop
Gore et al., J Biol Chem 2008 : We show that in B lymphocytes, MIF initiates a signaling cascade that involves Syk and Akt, leading to NF-kappaB activation, proliferation, and survival in a CD74- and CD44 dependent manner
Lee et al., American journal of physiology. Renal physiology 2008 : Finally, a TGF-beta1 antibody and annexin V attenuated the reduction in nuclear translocation of NF-kappaB by sevoflurane
Kim et al., Biol Pharm Bull 2007 : Furthermore, poncirin inhibited the LPS induced DNA binding activity of nuclear factor-kappaB (NF-kappaB)
Sumi et al., Yakugaku Zasshi 2007 : Although IKKbeta activates transcription factor NF-kappaB and PTP1B negatively regulates the receptor-protein tyrosine kinase , such as epidermal growth factor receptor (EGFR) in cells, covalent modification of these proteins caused by 1,2-NQ results in inhibition of NF-kappaB activity and transactivation of EGFR ... Although IKKbeta activates transcription factor NF-kappaB and PTP1B negatively regulates the receptor-protein tyrosine kinase, such as epidermal growth factor receptor (EGFR) in cells, covalent modification of these proteins caused by 1,2-NQ results in inhibition of NF-kappaB activity and transactivation of EGFR
Liu et al., Int J Cancer 2008 (Carcinoma, Squamous Cell...) : Our previous study showed that aspirin induced apoptosis of esophageal cancer cells in vitro by inhibiting the pathway of NF-kappaB downstream regulation of cyclooxygenase-2 ... We conclude that meloxicam induces apoptosis in SCC of the esophagus in vivo by inhibiting the pathway of NF-kappaB downstream regulation of COX-2
Wang et al., J Neurosci Res 2008 : RAGE knockdown inhibited retinoic acid induced activation and blocked nuclear translocation of NF-kappaB, suggesting RAGE regulates activation of NF-kappaB
Lewis et al., J Invest Dermatol 2008 : UVB induced activation of NF-kappaB is regulated by the IGF-1R and dependent on p38 MAPK ... UVB induced activation of NF-kappaB is regulated by the IGF-1R and dependent on p38 MAPK
Choy et al., J Ethnopharmacol 2008 (Inflammation) : Furthermore, SDEE suppressed the LPS induced p65 expression of NF-kappa B , which was associated with the inhibition of I kappa B-alpha degradation
Rivas et al., Exp Cell Res 2008 (Carcinoma, Ductal, Breast...) : We demonstrate that TNFalpha induced proliferation of C4HD murine mammary tumor cells and of T47D cells through the activation of p42/p44 MAPK, JNK, PI3-K/Akt pathways and nuclear factor-kappa B (NF-kappa B) transcriptional activation
Cai et al., Exp Eye Res 2008 (Translocation, Genetic) : Induction of interleukin-6 in human retinal epithelial cells by an attenuated Herpes simplex virus vector requires viral replication and NFkappaB activation ... Inhibition of NF-kappaB translocation by an Adenovirus vector expressing a dominant negative IkappaB ( AdIkappaBam ) inhibited IL-6 up-regulation, indicating that NF-kappaB plays a role in increasing IL-6 expression in APRE-19 cells
Chiu et al., Mol Immunol 2008 : Thrombin induced IL-6 production in human synovial fibroblasts is mediated by PAR1, phospholipase C, protein kinase C alpha, c-Src, NF-kappa B and p300 pathway
Guo et al., Free Radic Biol Med 2008 : Avenanthramides, polyphenols from oats, inhibit IL-1beta induced NF-kappaB activation in endothelial cells ... CH3-Avn-c, a synthetically prepared methyl ester derivative of Avn-c with a high biological potency, significantly and dose dependently decreased mRNA expression and secretion of IL-6, IL-8, and MCP-1 by HAEC as determined by real-time RT-PCR and ELISA, and it inhibited IL-1beta- and TNFalpha stimulated NF-kappaB activation as determined by a NF-kappaB DNA binding assay and a NF-kappaB luciferase reporter assay ... CH3-Avn-c, a synthetically prepared methyl ester derivative of Avn-c with a high biological potency, significantly and dose dependently decreased mRNA expression and secretion of IL-6 , IL-8, and MCP-1 by HAEC as determined by real-time RT-PCR and ELISA, and it inhibited IL-1beta- and TNFalpha stimulated NF-kappaB activation as determined by a NF-kappaB DNA binding assay and a NF-kappaB luciferase reporter assay ... CH3-Avn-c, a synthetically prepared methyl ester derivative of Avn-c with a high biological potency, significantly and dose dependently decreased mRNA expression and secretion of IL-6, IL-8 , and MCP-1 by HAEC as determined by real-time RT-PCR and ELISA, and it inhibited IL-1beta- and TNFalpha stimulated NF-kappaB activation as determined by a NF-kappaB DNA binding assay and a NF-kappaB luciferase reporter assay ... CH3-Avn-c, a synthetically prepared methyl ester derivative of Avn-c with a high biological potency, significantly and dose dependently decreased mRNA expression and secretion of IL-6, IL-8, and MCP-1 by HAEC as determined by real-time RT-PCR and ELISA, and it inhibited IL-1beta- and TNFalpha stimulated NF-kappaB activation as determined by a NF-kappaB DNA binding assay and a NF-kappaB luciferase reporter assay
Domingo-Domènech et al., Breast Cancer Res Treat 2008 (Breast Neoplasms) : We observed that the HDACi suberoylanilide hydroxamic acid ( SAHA ) or trichostatin A (TSA) induced cell death but also enhanced NF-kappaB-activity
Reddy et al., J Cell Physiol 2008 : Interleukin-18 stimulates fibronectin expression in primary human cardiac fibroblasts via PI3K-Akt dependent NF-kappaB activation ... Interleukin-18 stimulates fibronectin expression in primary human cardiac fibroblasts via PI3K-Akt dependent NF-kappaB activation ... IL-18 stimulated in vitro ( p65, p50 ) and in vivo NF-kappaB DNA binding activities, and induced kappaB dependent reporter gene activity
Kako et al., J Pharm Sci 2008 (Inflammation) : It is also suggested that TNF-alpha activates NF-kappaB and increases transgene expression by pDNA having many NF-kappaB binding sites, but TNF-alpha also reduces transgene expression at later time periods, leading to short-term transgene expression
Leclerc et al., J Biol Chem 2008 : We recently demonstrated that MTHFR can be up-regulated by NF-kappaB , an important mediator of cell survival that is activated by endoplasmic reticulum ( ER ) stress
Wang et al., Biochemistry 2008 : In a kinase gene screen using a NF-kappaB reporter, we observed that overexpression of casein kinase 1alpha (CK1alpha) enhanced TNFalpha induced NF-kappaB activation, and a CK1alpha kinase dead mutant, CK1alpha ( K46A ), reduced NF-kappaB activation induced by TNFalpha ... In a kinase gene screen using a NF-kappaB reporter, we observed that overexpression of casein kinase 1alpha (CK1alpha) enhanced TNFalpha induced NF-kappaB activation, and a CK1alpha kinase dead mutant, CK1alpha ( K46A ), reduced NF-kappaB activation induced by TNFalpha ... In a kinase gene screen using a NF-kappaB reporter, we observed that overexpression of casein kinase 1alpha (CK1alpha) enhanced TNFalpha induced NF-kappaB activation, and a CK1alpha kinase dead mutant, CK1alpha ( K46A ), reduced NF-kappaB activation induced by TNFalpha ... In a kinase gene screen using a NF-kappaB reporter, we observed that overexpression of casein kinase 1alpha (CK1alpha) enhanced TNFalpha induced NF-kappaB activation, and a CK1alpha kinase dead mutant, CK1alpha ( K46A ), reduced NF-kappaB activation induced by TNFalpha ... Finally, we showed that CK1alpha enhanced RIP1 mediated NF-kappaB activation ... Finally, we showed that CK1alpha enhanced RIP1 mediated NF-kappaB activation
Yin et al., Life Sci 2008 (Myocardial Infarction...) : Furthermore, kallikrein reduced I/R induced JNK, p38MAPK, IkappaB-alpha phosphorylation and nuclear NF-kappaB activation
Wang et al., Mol Immunol 2008 : Previous studies have shown that NIK phosphorylates IKK alpha at Ser-176 and Ser-180 residues, and mutation of each of the two residues to glutamate, which mimics its phosphorylation, caused constitutive activation of NF-kappaB ... These findings suggest that IKK alpha mediated activation of NF-kappaB and IRF3/7 are differentially regulated by NIK, and NIK plays an important role in TLR7/9 mediated IFN-alpha production
Mitsuhashi et al., Bioorg Med Chem 2008 : In this paper, we exhibit that ( i ) HIV-1gene expression was inhibited by lignin, ( ii ) fraction of small molecular mass in HBS lignin ( less than 0.5kDa ) had stronger inhibitory effects than large molecular mass ( more than 1.3kDa ), ( iii ) lignin also inhibited activation of NF-kappaB induced by TNFalpha , ( iv ) among six lignin dimer-like compounds, compound 6 containing beta-5 bond has more potent inhibitory activity than compounds 1, 2, 3, 4 and 5, which contain beta-1, beta-O-4, 5-5, or beta-beta structural units
Trivedi et al., Gene 2008 : Differential regulation of HOXA9 expression by nuclear factor kappa B (NF-kappaB) and HOXA9
Li et al., Cytokine 2008 : Positive and negative signaling components involved in TNFalpha induced NF-kappaB activation ... Stimulation of cells with TNFalpha triggers activation of NF-kappaB through various signaling molecules, including TRAF2, RIP, MAP3K, and the IKK complex
Zhou et al., Allergy 2008 (Asthma...) : Significantly, AdIkappaBalphaM inhibited CD3/CD28 mediated NF-kappaB activation in CD45RO ( + ) T cells, leading to evident apoptosis, reduction of eotaxin, RANTES, Th1 [ interferon (IFN)-gamma and interleukin (IL)-2 ], and Th2 ( IL-4, IL-5, and IL-13 despite a slight decrease in IL-10 ) cytokines and secondary proliferative response
Piao et al., J Biol Chem 2008 : Mutagenesis of Tyr-86, Tyr-106, and Tyr-159 residues within the Toll-IL-1 receptor domain impaired Mal tyrosine phosphorylation, interactions with Bruton tyrosine kinase, phosphorylation of p38 , and NF-kappaB activation
Hsieh et al., Toxicological sciences : an official journal of the Society of Toxicology 2008 : Statin induced heme oxygenase-1 increases NF-kappaB activation and oxygen radical production in cultured neuronal cells exposed to lipopolysaccharide ... Inhibition of the induced HO-1 by zinc protoporphyrin reduced the increased NF-kappaB activation and superoxides production ... This study revealed that simvastatin induced HO-1 led to increased NF-kappaB activation and superoxides production in the neuronal cells when exposed to LPS, and iron production may play a role in such a response
Wei et al., Am J Physiol Endocrinol Metab 2008 (Insulin Resistance) : Angiotensin II-induced skeletal muscle insulin resistance mediated by NF-kappaB activation via NADPH oxidase ... Moreover, ANG II treatment of L6 myotubes induced NF-kappaB activation and TNF-alpha production and decreased insulin stimulated Akt activation and GLUT-4 glucose transporter translocation to plasma membranes ... Moreover, ANG II treatment of L6 myotubes induced NF-kappaB activation and TNF-alpha production and decreased insulin stimulated Akt activation and GLUT-4 glucose transporter translocation to plasma membranes
Benayoun et al., FASEB J 2008 (Muscular Dystrophies, Limb-Girdle) : NF-kappaB dependent expression of the antiapoptotic factor c-FLIP is regulated by calpain 3, the protein involved in limb-girdle muscular dystrophy type 2A
Takase et al., Kidney Int 2008 (Chronic Disease...) : Inhibition of NF-kappaB dependent Bcl-xL expression by clusterin promotes albumin induced tubular cell apoptosis
Nagy et al., J Mol Cell Cardiol 2008 (Myocardial Reperfusion Injury...) : Glrx2 mediated survival signal appeared to be stemmed from PI-3-kinase-Akt survival signaling pathway and involved the activation of redox sensitive transcription factor NFkappaB and antiapoptotic protein Bcl-2
Tiruppathi et al., J Biol Chem 2008 : Role of NF-kappaB dependent caveolin-1 expression in the mechanism of increased endothelial permeability induced by lipopolysaccharide ... Thus, LPS mediates NF-kappaB dependent Cav-1 expression that results in increased caveolae number and thereby contributes to the mechanism of increased transendothelial albumin permeability
Kubo-Murai et al., J Biochem 2008 : IRAK-4 dependent degradation of IRAK-1 is a negative feedback signal for TLR mediated NF-kappaB activation ... These results suggested that the expression of IRAK-4 alone is sufficient to cause the degradation of IRAK-1 ; the autophosphorylation of IRAK-1 is not necessary to terminate the TLR induced activation of NF-kappaB
Mullan et al., Am J Respir Cell Mol Biol 2008 : beta-tryptase also up-regulated IL-8 mRNA expression, as analyzed by RT-PCR and real-time PCR, which was abolished by the transcription inhibitor actinomycin D. Reporter gene assay showed that beta-tryptase induced IL-8 transcription was mediated by the transcription factors activator protein-1, CCAAT/enhancer binding protein, and NF-kappaB , and chromatin immunoprecipitation assay demonstrated that beta-tryptase induced in vivo binding of these transcription factors to the IL-8 gene promoter
Yang et al., Molecular vision 2007 (Radiation Injuries) : These results also suggest that p38 , p44/42, and JNK may regulate glial induced photoreceptor death and that p38, JNK, and NF-kappaB may regulate photoreceptor induced microglial activation ... These results also suggest that p38, p44/42 , and JNK may regulate glial induced photoreceptor death and that p38, JNK, and NF-kappaB may regulate photoreceptor induced microglial activation ... These results also suggest that p38, p44/42 , and JNK may regulate glial induced photoreceptor death and that p38, JNK, and NF-kappaB may regulate photoreceptor induced microglial activation
Hasegawa et al., EMBO J 2008 : A critical role of RICK/RIP2 polyubiquitination in Nod induced NF-kappaB activation ... Nod1 and Nod2 stimulation activates NF-kappaB through RICK, a caspase-recruitment domain containing kinase ... However, the mechanism by which RICK activates NF-kappaB in response to Nod1 and Nod2 stimulation is unknown ... However, the mechanism by which RICK activates NF-kappaB in response to Nod1 and Nod2 stimulation is unknown ... Thus, RICK polyubiquitination links TAK1 to IKK complexes, a critical step in Nod1/Nod2 mediated NF-kappaB activation ... Thus, RICK polyubiquitination links TAK1 to IKK complexes, a critical step in Nod1/Nod2 mediated NF-kappaB activation
Yan et al., Mol Cell Biochem 2008 (Prostatic Neoplasms) : YXQG-EQ treatment inhibited constitutive and epidermal growth factor induced Akt phosphorylation, basal and TNF-alpha induced NF-kappaB activation, and downregulated anti-apoptotic Bcl-2 and Bcl-xL expression
Shifera et al., Mol Cell Biochem 2008 : Even though IKK gamma is required for the activation of NF-kappa B , we found that it reduced the activity of NF-kappa B when it was overexpressed
Hinshaw-Makepeace et al., Eur J Immunol 2008 : Effective stimulation of NF-kappaB in T cells following TCR ligation requires the activity of caspase-8
Verstrepen et al., J Cell Mol Med 2008 (Sepsis) : We have previously identified A20 binding inhibitor of NF-kappaB activation ( ABIN)-3 as an lipopolysaccharide (LPS)-inducible protein in monocytes that negatively regulates NF-B activation in response to tumour necrosis factor (TNF) and LPS ... This revealed that the LPS- and TNF-inducible expression of ABIN-3 is dependent on the binding of NF-kappaB to a specific B site in the ABIN-3 promoter ... This revealed that the LPS- and TNF-inducible expression of ABIN-3 is dependent on the binding of NF-kappaB to a specific B site in the ABIN-3 promoter ... This revealed that the LPS- and TNF-inducible expression of ABIN-3 is dependent on the binding of NF-kappaB to a specific B site in the ABIN-3 promoter ... Altogether, these data indicate an important role for NF-kappaB dependent gene expression of ABIN-3 in the negative feedback regulation of TNF receptor and toll-like receptor 4 induced NF-kappaB activation ... Altogether, these data indicate an important role for NF-kappaB dependent gene expression of ABIN-3 in the negative feedback regulation of TNF receptor and toll-like receptor 4 induced NF-kappaB activation
Hu et al., Eur J Pharmacol 2008 : Berberine attenuated RANKL induced activation of NF-kappaB through inhibiting phosphorylation at the activation loop of IkappaBalpha kinase beta, phosphorylation and degradation of IkappaBalpha, and NF-kappaB p65 nuclear translocation
Liu et al., Blood 2008 (Leukemia, Myeloid, Acute) : The involvement of Sp1/NF-kappaB in DNMT1 regulation is further demonstrated by the observation that Sp1 knockdown using mithramycin A or shRNA decreases DNMT1 protein levels, which instead are increased by Sp1 or NF-kappaB overexpression
Palaga et al., Eur J Immunol 2008 : Taken together, stimulation of macrophages through the TLR signaling cascade triggered activation of Notch signaling, which in turn regulated gene expression patterns involved in pro-inflammatory responses, through activation of NF-kappaB
Folmer et al., J Nat Prod 2008 : Both naphthopyrones 1 and 2 completely inhibit TNF-alpha induced NF-kappaB activation at a concentration of 300 microM by inhibiting the enzymatic activity of the kinase IKKbeta ... Both naphthopyrones 1 and 2 completely inhibit TNF-alpha induced NF-kappaB activation at a concentration of 300 microM by inhibiting the enzymatic activity of the kinase IKKbeta
Mendonca et al., Radiat Res 2007 : Treatment with 5 microM parthenolide for 48 to 72 h inhibited constitutive NF-kappaB binding and cell growth, reduced plating efficiency, and induced apoptosis through stabilization of p53 (TP53), induction of the pro-apoptosis protein BAX , and phosphorylation of BID
Lee et al., Biochem Biophys Res Commun 2008 (MAP Kinase Signaling System...) : Suppression of PMA induced tumor cell invasion by capillarisin via the inhibition of NF-kappaB dependent MMP-9 expression ... Capillarisin has been found to suppress PMA induced MMP-9 expression through inhibition of the NF-kappaB dependent transcriptional activity of MMP-9 gene via p38 MAPK and JNK signaling pathways ... These results suggest that capillarisin represents a potential anti-metastatic agent suppressing cancer cell invasion through specific inhibition of NF-kappaB dependent MMP-9 gene expression
Shareef et al., Cancer Res 2007 (Adenocarcinoma...) : These findings show that the reduced bystander response in A549 cells is due to activation of NF-kappaB signaling by TNF-alpha , whereas enhanced response to IR-induced bystander signaling in H460 cells was due to release of TRAIL associated with nuclear translocation of PAR-4
Shu et al., Cancer Res 2007 (Disease Models, Animal...) : Parathyroid hormone related protein ( PTHrP ) and macrophage inflammatory protein-1 alpha ( MIP-1 alpha ) are important factors in the pathogenesis of HHM in ATLL and the expression of PTHrP can be activated by nuclear factor kappaB (NF-kappaB) ... Parathyroid hormone related protein ( PTHrP ) and macrophage inflammatory protein-1 alpha ( MIP-1 alpha ) are important factors in the pathogenesis of HHM in ATLL and the expression of PTHrP can be activated by nuclear factor kappaB (NF-kappaB)
Preciado et al., Laryngoscope 2008 : Cigarette smoke activates NF kappa B and induces Muc5b expression in mouse middle ear cells ... We hypothesized that in vitro stimulation of a recently developed model system, murine middle ear epithelial cells ( MEEC ), with cigarette smoke condensate ( CSC ) activates NF-kappa B and subsequently induces Muc5b gene expression
Scicchitano et al., Blood Cells Mol Dis 2008 (MAP Kinase Signaling System) : In addition, these results suggest that IL-6, IL-11, GM-CSF , G-CSF and Activin A are similarly regulated by p38 and NF-kappaB and that the MEK1, JNK and PKC pathways appear to play a more limited role in modulating cytokine expression in HBMSC ... In addition, these results suggest that IL-6, IL-11, GM-CSF, G-CSF and Activin A are similarly regulated by p38 and NF-kappaB and that the MEK1, JNK and PKC pathways appear to play a more limited role in modulating cytokine expression in HBMSC ... In addition, these results suggest that IL-6, IL-11 , GM-CSF, G-CSF and Activin A are similarly regulated by p38 and NF-kappaB and that the MEK1, JNK and PKC pathways appear to play a more limited role in modulating cytokine expression in HBMSC ... In addition, these results suggest that IL-6 , IL-11, GM-CSF, G-CSF and Activin A are similarly regulated by p38 and NF-kappaB and that the MEK1, JNK and PKC pathways appear to play a more limited role in modulating cytokine expression in HBMSC ... In addition, these results suggest that IL-6, IL-11, GM-CSF, G-CSF and Activin A are similarly regulated by p38 and NF-kappaB and that the MEK1, JNK and PKC pathways appear to play a more limited role in modulating cytokine expression in HBMSC
Matsuda et al., Biochem Pharmacol 2008 : We conclude that the priming effect of LPS on PepG induced lung injury and death is preceded by NF-kappaB mediated up-regulation of TLR-2
Tanaka et al., J Hepatol 2008 (Liver Failure, Acute) : FR183998 prevented the activation of transcription factor NF-kappaB induced by GalN/LPS ... FR183998 prevented the activation of transcription factor NF-kappaB induced by GalN/LPS
Blanco et al., J Immunol 2008 : Interestingly, Tat did not induce NF-kappaB activity, suggesting that NF-kappaB was not necessary to control COX-2 transcription induced by Tat ... Interestingly, Tat did not induce NF-kappaB activity, suggesting that NF-kappaB was not necessary to control COX-2 transcription induced by Tat
Wang et al., Chem Biol Interact 2008 (Inflammation) : PCB77 induced oxidative stress and activation of the oxidative stress sensitive transcription factor nuclear factor kappaB (NF-kappaB) were markedly increased in the presence of LA and diminished by increasing the relative amount of ALA to LA
Puthia et al., Eukaryotic cell 2008 (Blastocystis Infections) : Furthermore, we show that NF-kappaB activation is involved in the production of IL-8
Cho et al., J Nutr 2008 (Inflammation) : In addition, DIM suppressed LPS induced nuclear factor-kappaB (NF-kappaB) transcriptional activity, NF-kappaB DNA binding activity, translocation of p65 ( RelA ) to the nucleus, and degradation of inhibitor of kappaB alpha
Manea et al., Arch Physiol Biochem 2007 : Regulation of NADPH oxidase subunit p22(phox) by NF-kB in human aortic smooth muscle cells ... Here we investigate the role of NF-kB in the regulation of p22(phox) subunit and NADPH oxidase activity, in human aortic smooth muscle cells ... Regulation of NADPH oxidase by NF-kB may represent a possible mechanism whereby pro-inflammatory factors induce oxidative stress in atherosclerosis, hypertension, diabetes, stroke or heart failure
Huang et al., Nan Fang Yi Ke Da Xue Xue Bao 2007 (Carcinoma, Hepatocellular...) : NF-kappaB P65 siRNA induced obvious cell apoptosis with down-regulated Bcl-2 and up-regulated Bax expressions
Ramnath et al., Am J Physiol Cell Physiol 2008 : However, the signaling mechanisms by which the SP-NK1R interaction induces NF-kappaB activation and chemokine production remain unclear
Lee et al., American journal of physiology. Renal physiology 2008 : Consequently, the inhibition of Ca ( 2+ ), PKC, EGFR, p44/42 MAPKs, or NF-kappaB blocked the BSA induced increases in cyclin D1, cyclin dependent kinase (CDK)4, cyclin E, or CDK2 and restored the BSA induced inhibition of p21 ( WAF/Cip1 ) and p27 ( Kip1 ) expression ... Consequently, the inhibition of Ca ( 2+ ), PKC, EGFR, p44/42 MAPKs, or NF-kappaB blocked the BSA induced increases in cyclin D1 , cyclin dependent kinase (CDK)4, cyclin E, or CDK2 and restored the BSA induced inhibition of p21 ( WAF/Cip1 ) and p27 ( Kip1 ) expression ... Consequently, the inhibition of Ca ( 2+ ), PKC, EGFR, p44/42 MAPKs, or NF-kappaB blocked the BSA induced increases in cyclin D1, cyclin dependent kinase (CDK)4, cyclin E, or CDK2 and restored the BSA induced inhibition of p21 ( WAF/Cip1 ) and p27 ( Kip1 ) expression ... Consequently, the inhibition of Ca ( 2+ ), PKC, EGFR, p44/42 MAPKs, or NF-kappaB blocked the BSA induced increases in cyclin D1, cyclin dependent kinase (CDK)4 , cyclin E, or CDK2 and restored the BSA induced inhibition of p21 ( WAF/Cip1 ) and p27 ( Kip1 ) expression ... Consequently, the inhibition of Ca ( 2+ ), PKC, EGFR, p44/42 MAPKs, or NF-kappaB blocked the BSA induced increases in cyclin D1, cyclin dependent kinase (CDK)4, cyclin E, or CDK2 and restored the BSA induced inhibition of p21 ( WAF/Cip1 ) and p27 ( Kip1 ) expression ... Consequently, the inhibition of Ca ( 2+ ), PKC, EGFR, p44/42 MAPKs, or NF-kappaB blocked the BSA induced increases in cyclin D1, cyclin dependent kinase (CDK)4, cyclin E, or CDK2 and restored the BSA induced inhibition of p21 ( WAF/Cip1 ) and p27 ( Kip1 ) expression ... Consequently, the inhibition of Ca ( 2+ ), PKC, EGFR, p44/42 MAPKs, or NF-kappaB blocked the BSA induced increases in cyclin D1, cyclin dependent kinase (CDK)4 , cyclin E, or CDK2 and restored the BSA induced inhibition of p21 ( WAF/Cip1 ) and p27 ( Kip1 ) expression ... In conclusion, BSA stimulates DNA synthesis that is mediated by Ca ( 2+ ) /PKC as well as the EGFR dependent p44/42 MAPK and NF-kappaB signal pathways in PTCs
Ye et al., Mol Cell Biol 2008 : These motifs are also required for Monarch-1 to undergo self-oligomerization, Toll-like receptor- or CD40L activated association with NF-kappaB inducing kinase (NIK) and interleukin-1 receptor associated kinase 1 ( IRAK-1 ), degradation of NIK, and inhibition of IRAK-1 phosphorylation
Dufner et al., Mol Cell Biol 2008 : RIP2 mediates NF-kappaB activation induced by the intracellular nucleotide binding oligomerization domain ( NOD ) receptors that sense bacterial peptidoglycan ... RIP2 mediates NF-kappaB activation induced by the intracellular nucleotide binding oligomerization domain ( NOD ) receptors that sense bacterial peptidoglycan
Jung et al., Exp Mol Med 2007 (Inflammation) : The results indicate that celastrol effectively attenuated NO and proinflammatory cytokine production via the inhibition of ERK1/2 phosphorylation and NF-kappaB activation in LPS activated microglia
Song et al., Exp Mol Med 2007 (HIV Infections) : Treatment of astrocytes with cell-permeable SOD led to decrease in Tat induced ROS generation as well as NF-kappaB activation ... These data indicate that SOD has a regulatory function for HIV-1 Tat induced NF-kappaB activation in astrocytes and suggest that cell-permeable SOD can be used as a feasible therapeutic agent for regulation of ROS related neurological diseases
Baugé et al., Arthritis Rheum 2008 : In addition, transient transfections of p65 expression vector or p65 targeted short hairpin RNA were performed to define the effect of NF-kappaB on Smad7 expression
Rodríguez-Calvo et al., J Gerontol A Biol Sci Med Sci 2007 (Cardiomegaly) : These findings suggest that NF-kappaB activation in SAM-P8 heart prevents PPARalpha from binding to its response elements leading to changes in gene expression that may lead to ceramide accumulation in the aged heart
Yang et al., J Biol Chem 2008 (MAP Kinase Signaling System) : Lastly, we examined the role of the immediate-early transcription factors AP-1 and NF-kappaB and have found that phospholipase C-gamma dependent c-Jun and ERK dependent c-fos, but not the NF-kappaB , are strongly activated by bFGF , which in turn regulates the neuronal differentiation of BMSCs ... Lastly, we examined the role of the immediate-early transcription factors AP-1 and NF-kappaB and have found that phospholipase C-gamma dependent c-Jun and ERK dependent c-fos, but not the NF-kappaB , are strongly activated by bFGF, which in turn regulates the neuronal differentiation of BMSCs
Lee et al., Int J Cancer 2008 (Carcinoma, Squamous Cell...) : A signal network involving coactivated NF-kappaB and STAT3 and altered p53 modulates BAX/BCL-XL expression and promotes cell survival of head and neck squamous cell carcinomas ... A signal network involving coactivated NF-kappaB and STAT3 and altered p53 modulates BAX/BCL-XL expression and promotes cell survival of head and neck squamous cell carcinomas ... Greater modulation among HNSCC lines expressing low wt p53 than those over expressing mt p53 protein suggested that decreased p53 expression might enhance activation of NF-kappaB , STAT3 and BCL-XL
Williams et al., Carcinogenesis 2008 (Adenocarcinoma) : We studied in several human colon ( HT-29, HCT-15, LoVo, HCT116 and SW-480 ), pancreatic ( BxPC-3, MIA PaCa-2 ) and breast ( MDA-MB-231 and MCF-7 ) cancer cell lines, the effect of NO-ASA on NF-kappaB activation, determined by electrophoretic mobility shift assays, immunofluorescence and western blot analyses of nuclear proteins ... The effect of NO-ASA on NF-kappaB binding to DNA was significantly correlated with its effect on cell growth ( P < 0.05 ) indicating that the growth inhibitory effect of NO-ASA may be mediated by its effect on NF-kappaB
Lu et al., J Leukoc Biol 2008 (Pneumonia, Bacterial...) : RSV was shown to ameliorate acute pneumonia via inhibition of the NF-kappaB signaling pathway, including inhibition of IkappaBalpha phosphorylation and subsequent NF-kappaB activation
Viña et al., Biol Chem 2008 : Activation of NF-kappa B by estrogens subsequently activates the expression of Mn-SOD and GPx, but genistein is only capable of activating Mn-SOD expression ... Activation of NF-kappa B by estrogens subsequently activates the expression of Mn-SOD and GPx , but genistein is only capable of activating Mn-SOD expression
You et al., FEMS Immunol Med Microbiol 2008 : The DNA binding activity of NF-kappaB and AP-1 was also assessed by an electrophoretic mobility gel shift assay, and an NF-kappaB specific inhibitor, pyrrolidine dithiocarbamate, profoundly inhibited the synthesis and production of the proinflammatory cytokines
Méndez-Samperio et al., Cell Immunol 2007 (Tuberculosis) : Sphingosine kinase, phosphatidylinositol 3-kinase, Akt, NF-kappaB , and p300 are required for CCL5 production in Mycobacterium bovis Bacillus Calmette-Guérin ( BCG ) -infected epithelial cells
Amigó et al., Life Sci 2008 (Disease Models, Animal...) : Avarol inhibited tumor necrosis factor-alpha (TNF-alpha) generation in stimulated human monocytes ( IC ( 50 ) 1 microM ) and TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB)-DNA binding in keratinocytes
Liao et al., Leuk Res 2008 : Meanwhile conventional expression of ODC represses tumor necrosis factor-alpha (TNF-alpha) expression and nuclear factor-kappaB (NF-kappaB) activation as well as MMP-9 enzyme activity ... In addition, the NF-kappaB inhibitors ( pyrrolidinedithiocarbamate, BAY-11-7082 and lactacystin ) suppress the TPA induced MMP-9 enzyme activity ... Furthermore, ODC could directly inhibit and attenuate NF-kappaB DNA binding and transcriptional activation
Souto-Carneiro et al., J Immunol 2008 (Ectodermal Dysplasia 1, Anhidrotic...) : Although NF-kappaB binding sites are present in the human and mouse IgH, Igkappa, and Iglambda enhancer modules and RAG expression is controlled by NF-kappaB , it is not known whether NF-kappaB regulates V ( D ) J recombination mechanisms after RAG mediated dsDNA breaks
Nareika et al., J Endocrinol 2008 : Further investigations using transcription factor activity assays and gel shift assays revealed that high glucose augmented LPS stimulated CD14 expression by enhancing transcription factor nuclear factor kappaB (NFkappaB) and activator protein-1 (AP-1) activities
Rockel et al., Arthritis Res Ther 2008 : Tumour necrosis factor (TNF)-alpha , found in arthritic joints, activates nuclear factor-kappaB (NF-kappaB) , whereas retinoic acid receptors (RARs) are activated by retinoid agonists, including all-trans retinoic acid ( atRA )
Lin et al., Toxicol Appl Pharmacol 2008 : In our previous study, LPS has been shown to induce vascular cell adhesion molecule-1 ( VCAM-1 ) expression through MAPKs and NF-kappaB in human tracheal smooth muscle cells ( HTSMCs )
Lee et al., Int Immunopharmacol 2008 : Alpha-lipoic acid inhibits TNF-alpha induced NF-kappa B activation through blocking of MEKK1-MKK4-IKK signaling cascades
Veber et al., J Neurosci Res 2008 (Vitamin B 12 Deficiency) : We used electrophoretic mobility shift assays to investigate the effects of cobalamin (Cbl) deficiency on the levels of activated nuclear factor-kappa B (NF-kappaB) in the spinal cords ( SCs ) and livers of rats made Cbl-deficient (Cbl-D) by total gastrectomy or a Cbl-D diet
Terada et al., J Am Soc Nephrol 2008 (Fibrosis) : In primary cultures of rat mesangial cells, aldosterone stimulated the expression, phosphorylation, and kinase activity of SGK1, as well as SGK1 dependent NF-kappaB activity ... Furthermore, aldosterone augmented the promoter activity and protein expression of intercellular adhesion molecule-1 ( ICAM-1 ), which modulates the inflammatory response, and the profibrotic cytokine connective tissue growth factor (CTGF) in an SGK1- and NF-kappaB dependent manner ... Furthermore, aldosterone augmented the promoter activity and protein expression of intercellular adhesion molecule-1 ( ICAM-1 ), which modulates the inflammatory response, and the profibrotic cytokine connective tissue growth factor (CTGF) in an SGK1- and NF-kappaB dependent manner
Adya et al., Diabetes Care 2008 (Diabetes Mellitus, Type 2...) : We investigated the activation of nuclear factor-kappaB (NF-kappaB) , a well-known proinflammatory transcription factor, by visfatin in endothelial cells ... Visfatin significantly increased NF-kappaB transcriptional activity ( P < 0.001 ) ... We also found a significant inhibition of tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB activity by visfatin ( P < 0.001 ) ... Visfatin induced NF-kappaB signaling in human endothelial cells affects the activation of gelatinases MMP-2 and -9, suggesting an important role of visfatin in the pathogenesis of vascular inflammation in obesity and type 2 diabetes ... Visfatin induced NF-kappaB signaling in human endothelial cells affects the activation of gelatinases MMP-2 and -9, suggesting an important role of visfatin in the pathogenesis of vascular inflammation in obesity and type 2 diabetes ... Visfatin induced NF-kappaB signaling in human endothelial cells affects the activation of gelatinases MMP-2 and -9 , suggesting an important role of visfatin in the pathogenesis of vascular inflammation in obesity and type 2 diabetes
Wu et al., Toxicol Appl Pharmacol 2008 : Involvement of mitogen activated protein kinases and NFkappaB in LPS induced CD40 expression on human monocytic cells ... Further studies using immunoblotting and pharmacological inhibitors revealed that mitogen activated protein kinases ( MAPKs ) and NFkappaB were activated by LPS exposure and involved in LPS induced CD40 expression ... Activation of MAPKs was not responsible for LPS induced NFkappaB activation ... TLR4 was expressed on THP-1 cells and pretreatment of cells with a Toll-like receptor 4 (TLR4) neutralizing antibody ( HTA125 ) significantly blunted LPS induced MAPK and NFkappaB activation and ensuing CD40 expression ... Additional studies with murine macrophages expressing wild type and mutated TLR4 showed that TLR4 was implicated in LPS induced ERK and NFkappaB activation, and CD40 expression ... In summary, LPS induced CD40 expression in monocytic cells involves MAPKs and NFkappaB
Perez-Rosado et al., J Pathol 2008 (Lymphoma, Large B-Cell, Diffuse) : BCL6 represses NFkappaB activity in diffuse large B-cell lymphomas
Cezar-de-Mello et al., Br J Pharmacol 2008 (Neovascularization, Pathologic) : Because the transcription factor NF-kappaB has been implicated in VEGF mediated MMP expression and EC proliferation, we postulated that ATL-1 might modulate the NF-kappaB pathway and, indeed, ATL-1 inhibited NF-kappaB nuclear translocation
Kizaki et al., Immunology 2008 : The activation of nuclear factor-kappaB (NF-kappaB) and degradation of the inhibitor of NF-kappaB ( IkappaBalpha ) in response to LPS were markedly decreased in these cells ... These findings suggest that LPS stimulated signals suppress beta ( 2 ) AR expression, leading to down-regulation of beta-arrestin 2 expression, which stabilizes cytosolic IkappaBalpha and inhibits the NF-kappaB activation essential for NOS II expression, probably to ensure rapid and sufficient production of NO in response to microbial attack
Glauben et al., Gut 2008 (Colitis...) : As for potential mechanisms of ITF2357 action, increased acetylation of histone 3, reduced production of IFN gamma and enhanced apoptosis in lamina propria mononuclear cells were found to accompany a histone deacetylase dependent activation of NF-kappaB
Ou et al., Respirology 2008 : Moreover, LPS increased staining for NF-kappaB in the cytoplasm as well as nuclear translocation of NF-kappaB in airway epithelial cells
Schneider et al., PLoS Biol 2008 : The tumor necrosis factor (TNF)-receptor 1-associated death domain protein ( TRADD ) mediates induction of apoptosis as well as activation of NF-kappaB by cellular TNF-receptor 1 (TNFR1)
Nishi et al., Antioxid Redox Signal 2008 : LPS mediated activation of HIF-1 was independent of NF-kappaB activity
Lin et al., J Am Soc Nephrol 2008 (Acute Kidney Injury...) : Measures of inflammation ( number of infiltrating neutrophils, myeloperoxidase activity, and induction of IL-6 and P-selectin ) and activation of NF-kappaB were lower in GC-G ( -/- ) mice compared with wild-type mice ... Measures of inflammation ( number of infiltrating neutrophils, myeloperoxidase activity, and induction of IL-6 and P-selectin ) and activation of NF-kappaB were lower in GC-G ( -/- ) mice compared with wild-type mice ... Measures of inflammation ( number of infiltrating neutrophils, myeloperoxidase activity, and induction of IL-6 and P-selectin ) and activation of NF-kappaB were lower in GC-G ( -/- ) mice compared with wild-type mice
Scortegagna et al., Blood 2008 (Drug Hypersensitivity...) : HIF-1 induced NFkappaB activation was composed of 2 elements, IkappaB hyperphosphorylation and phosphorylation of Ser276 on p65, enhancing p65 nuclear localization and transcriptional activity, respectively
Li et al., Molecular vision 2007 : Inhibition of NF-kappaB partially prevented the induction of IL-1alpha, IL-8, and ELAM-1 , but not IL-6 ... Inhibition of NF-kappaB partially prevented the induction of IL-1alpha , IL-8, and ELAM-1, but not IL-6 ... Inhibition of NF-kappaB partially prevented the induction of IL-1alpha, IL-8, and ELAM-1, but not IL-6 ... Inhibition of NF-kappaB partially prevented the induction of IL-1alpha, IL-8 , and ELAM-1, but not IL-6
Rebholz et al., J Invest Dermatol 2008 (MAP Kinase Signaling System) : The biphasic NF-kappaB response was strictly dependent on the transactivating subunit RelA , as demonstrated by keratinocytes lacking RelA
Horiuchi et al., Drugs 2006 : Nifedipine inhibited MCP-1 expression by inhibiting nuclear factor (NF)-kappaB activation
Gong et al., J Biol Chem 2008 (Inflammation...) : Hepatocyte growth factor suppresses proinflammatory NFkappaB activation through GSK3beta inactivation in renal tubular epithelial cells ... Here we report that HGF through inactivation of GSK3beta suppresses NFkappaB p65 phosphorylation specifically at position Ser-468
Saha et al., Am J Physiol Gastrointest Liver Physiol 2008 (Helicobacter Infections...) : Helicobacter pylori induced H, K-ATPase alpha-subunit gene repression is mediated by NF-kappaB p50 homodimer promoter binding ... We conclude that H. pylori inhibits HKalpha gene expression by ERK1/2 mediated NF-kappaB p50 homodimer binding to the HKalpha promoter
Kim et al., Mol Pharmacol 2008 (Inflammation...) : BOT-64 attenuates NF-kappaB regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha , interleukin (IL)-1beta, and IL-6 in LPS activated or expression vector IKKbeta transfected macrophages ... BOT-64 attenuates NF-kappaB regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha, interleukin (IL)-1beta , and IL-6 in LPS activated or expression vector IKKbeta transfected macrophages ... Here, we demonstrate that the novel small-molecule benzoxathiole 6,6-dimethyl-2- ( phenylimino ) -6,7-dihydro-5H-benzo- [ 1,3 ] oxathiol-4-one ( BOT-64 ) inhibits NF-kappaB activation with an IC ( 50 ) value of 1 muM by blocking inhibitory kappaB(IkappaB) kinase beta ( IKKbeta ), and suppresses NF-kappaB regulated expression of inflammatory genes in lipopolysaccharide (LPS) activated RAW 264.7 macrophages ... BOT-64 attenuates NF-kappaB regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha, interleukin (IL)-1beta , and IL-6 in LPS activated or expression vector IKKbeta transfected macrophages ... BOT-64 attenuates NF-kappaB regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2, tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-6 in LPS activated or expression vector IKKbeta transfected macrophages ... BOT-64 attenuates NF-kappaB regulated expression of inflammatory genes such as inducible nitric-oxide synthase , cyclooxygenase-2, tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-6 in LPS activated or expression vector IKKbeta transfected macrophages ... BOT-64 attenuates NF-kappaB regulated expression of inflammatory genes such as inducible nitric-oxide synthase, cyclooxygenase-2 , tumor necrosis factor-alpha, interleukin (IL)-1beta, and IL-6 in LPS activated or expression vector IKKbeta transfected macrophages
Yoon et al., Cell Death Differ 2008 : Furthermore, TNF induced p65/RelA phosphorylation as well as transcriptional activity of nuclear factor-kappaB (NF-kappaB) was significantly downregulated in T6 ( -/- ) 3T3 cells
Fantuzzi et al., Blood 2008 (HIV Infections) : Furthermore, PC-PLC is required for NF-kB mediated CCL2 production triggered by R5 gp120
Mitchell et al., Mol Hum Reprod 2008 : Further, p65 binding to the NFkappaB-responsive IkappaBalpha promoter increased at labour and correlated strongly with TBP binding to the TATA-box of this gene
Hasnis et al., J Physiol Pharmacol 2007 : Mild, but not high, exposure to CS induced NF-kappaB in lymphocytes through IKK activation, I-kappaBalpha degradation, and the reduction in the intracellular glutathione levels ... Mild, but not high, exposure to CS induced NF-kappaB in lymphocytes through IKK activation, I-kappaBalpha degradation, and the reduction in the intracellular glutathione levels ... Reduction of intracellular peroxynitrite formation by the inhibition of the mitochondrial respiratory chain resulted in decreased activation of NF-kappaB by CS. NF-kappaB induced iNOS levels were increased in response to CS. Low levels of CS exposure induced classical NF-kappaB activation pathway in lymphocytes via intracellular formation of peroxynitrite, through a reaction between smoke derived NO and endogenously produced superoxide
Dhiman et al., Biochim Biophys Acta 2008 : Here, we report that NF-kappaB dependent antiapoptotic protein bfl-1/A1 plays an important role in this process
Di et al., Cell Signal 2008 : However, the mechanism by which MEKK3 mediates NF-kappaB signaling is not fully understood ... Expression of non phosphorylated TAK1 interferes with MEKK3 phosphorylation and NF-kappaB reporter activity induced by transient MEKK3 expression or TNFalpha stimulation ... Expression of non phosphorylated TAK1 interferes with MEKK3 phosphorylation and NF-kappaB reporter activity induced by transient MEKK3 expression or TNFalpha stimulation
Shifera et al., Mol Immunol 2008 (Immunologic Deficiency Syndromes) : The suppression of IL-2 induction was solely due to the inhibition of NF-kappaB activation as the mutations did not impair the activation of AP-1 and NFAT
Fakhrzadeh et al., Toxicol Appl Pharmacol 2008 (Pneumonia) : These data demonstrate that TNFalpha plays a role in NF-kappaB activation and toxicity
Klatt et al., J Immunol 2008 : Efficient HIV-1 transcription requires the induction of cellular transcription factors, such as NF-kappaB , and the viral factor Tat, which through the recruitment of P-TEFb enhances processive transcription
Lemster et al., J Immunol 2008 : CD56 cross linking without TCR ligation on CD56 ( + ) T cells resulted in extensive protein phosphorylation, NF-kappaB activation, and Bax down-regulation
Zhao et al., J Cardiovasc Pharmacol 2008 : Our findings suggest that aspirin can decrease inflammatory responses induced by ox-LDL, and the mechanism might be associated with NF-kappaB activation pathway and inhibition of p38 MAPK phosphorylation ... Our findings suggest that aspirin can decrease inflammatory responses induced by ox-LDL, and the mechanism might be associated with NF-kappaB activation pathway and inhibition of p38 MAPK phosphorylation
Son et al., J Cardiovasc Pharmacol 2008 : Roles of MAPK and NF-kappaB in interleukin-6 induction by lipopolysaccharide in vascular smooth muscle cells
Chuang et al., In Vivo 2007 (Uterine Cervical Neoplasms) : Coumarin induces cell cycle arrest and apoptosis in human cervical cancer HeLa cells through a mitochondria- and caspase-3 dependent mechanism and NF-kappaB down-regulation
Mi et al., Cell cycle (Georgetown, Tex.) 2008 : Functional studies of the iNOS promoter demonstrated that the synergistic interaction between LPS and hypoxia was mediated , at least in part, by the NFkappaB and the HIF-1 binding sites
Ramsey et al., Oncogene 2008 (Prostatic Neoplasms) : Copine-I represses NF-kappaB transcription by endoproteolysis of p65
Lu et al., J Hepatol 2008 (Disease Models, Animal...) : PDTC protects mice against BCG/LPS induced inflammatory liver injury through the repression of NF-kappaB mediated TNF-alpha release, while it seems to be detrimental in GalN/LPS induced apoptotic liver damage
Zabalgoitia et al., Free Radic Biol Med 2008 : Treatment with interleukin (IL)-18 increased NF-kappaB activation and PTEN induction , suppressed Akt activation, and stimulated EC death ... Overexpression of HO-1, treatment with HO-1 inducer hemin, or the CO donor cobalt ( III ) protoporphyrin IX all reversed IL-18 mediated NF-kappaB activation, PTEN induction, Akt suppression, and EC death ... Overexpression of HO-1, treatment with HO-1 inducer hemin, or the CO donor cobalt ( III ) protoporphyrin IX all reversed IL-18 mediated NF-kappaB activation, PTEN induction , Akt suppression, and EC death ... HO-1 overexpression, HO-1 induction, or treatment with heme metabolites all reverse IL-18 mediated p38alpha MAPK and NF-kappaB activation , PTEN induction, Akt suppression, and EC death ... HO-1 overexpression, HO-1 induction, or treatment with heme metabolites all reverse IL-18 mediated p38alpha MAPK and NF-kappaB activation, PTEN induction , Akt suppression, and EC death
Sgarbanti et al., J Virol 2008 : IRF-1 is required for full NF-kappaB transcriptional activity at the human immunodeficiency virus type 1 long terminal repeat enhancer ... In this work we demonstrate that IRF-1 is also required for full NF-kappaB transcriptional activity
Bloor et al., Proc Natl Acad Sci U S A 2008 : NF-kappaB activation occurs upon degradation of its inhibitor I-kappaB and requires prior phosphorylation of the inhibitor by I-kappaB kinase (IKK)
Pace et al., Immunology 2008 : The LPS increased the expression of TLR4 and induced both NF-kappaB and ERK1/2 activation ... The LPS increased the expression of TLR4 and induced both NF-kappaB and ERK1/2 activation ... The combined exposure of 16-HBE to CSE and LPS was associated with ERK activation rather than NF-kappaB activation and with a further increase of IL-8 release and of chemotactic activity toward neutrophils
Chai et al., Zhonghua Nei Ke Za Zhi 2007 (Disease Models, Animal...) : The expression of NF-kappaB and TNFalpha induced by NF-kappaB play an important role in the pathogenesis of pneumonia
Paradowska et al., Arch Immunol Ther Exp (Warsz) 2007 (Arthritis, Rheumatoid) : IL-17 binding to an IL-17 receptor expressed on epithelial, endothelial, and fibroblastic stromal cells triggers the activation of transcription factor NF-kappaB and mitogen activated protein kinase ( p-38 ), which in turn results in the secretion of IL-1, TNF-alpha, IL-6, IL-8, or prostaglandin E2
Skultetyova et al., Recent Pat Cardiovasc Drug Discov 2007 (Inflammation) : AT(1) receptor enhances the activation of nuclear factor NF-kappaB , which stimulates the production of proinflammatory cytokines
Kim et al., Biochem Biophys Res Commun 2008 : Some reports have demonstrated that EPA inhibits NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha or lipopolysaccharide (LPS) in various cells ... Some reports have demonstrated that EPA inhibits NF-kappaB activation induced by tumor necrosis factor (TNF)-alpha or lipopolysaccharide (LPS) in various cells
Dogusan et al., Diabetes 2008 : TLR3 signaling mediated PICex induced nuclear factor-kappaB (NF-kappaB) and IRF-3 activation and beta-cell apoptosis
Torres et al., Nat Cell Biol 2008 : We also found that Nanog binds to NFkappaB proteins ; however, Nanog binding inhibited transcriptional activity of NFkappaB proteins ... We conclude that Nanog represses the pro-differentiation activities of NFkappaB and cooperates with Stat3 to maintain pluripotency
Tokunaga et al., Inflamm Res 2007 : Modification of IkappaBalpha by taurine bromamine inhibits tumor necrosis factor alpha induced NF-kappaB activation ... TauBr inhibited degradation of IkappaBalpha and TNFalpha induced NF-kappaB activation
Terragni et al., BMC cell biology 2008 : Phosphatidylinositol 3-kinase signaling in proliferating cells maintains an anti-apoptotic transcriptional program mediated by inhibition of FOXO and non-canonical activation of NFkappaB transcription factors ... RelB was constitutively bound to promoter regions in cells maintained in serum, however binding decreased following PI 3-kinase inhibition, indicating that PI 3-kinase signaling activates NFkappaB via the non-canonical pathway in proliferating cells
Shankar et al., Molecular cancer 2008 (Neoplasm Metastasis...) : In xenogrfated tumors, curcumin upregulated the expression of TRAIL-R1/DR4 , TRAIL-R2/DR5, Bax, Bak, p21/WAF1, and p27/KIP1, and inhibited the activation of NFkappaB and its gene products such as cyclin D1, VEGF, uPA, MMP-2, MMP-9, Bcl-2 and Bcl-XL ... In xenogrfated tumors, curcumin upregulated the expression of TRAIL-R1/DR4, TRAIL-R2/DR5 , Bax, Bak, p21/WAF1, and p27/KIP1, and inhibited the activation of NFkappaB and its gene products such as cyclin D1, VEGF, uPA, MMP-2, MMP-9, Bcl-2 and Bcl-XL ... In xenogrfated tumors, curcumin upregulated the expression of TRAIL-R1/DR4, TRAIL-R2/DR5, Bax, Bak, p21/WAF1, and p27/KIP1 , and inhibited the activation of NFkappaB and its gene products such as cyclin D1, VEGF, uPA, MMP-2, MMP-9, Bcl-2 and Bcl-XL ... In xenogrfated tumors, curcumin upregulated the expression of TRAIL-R1/DR4, TRAIL-R2/DR5, Bax, Bak, p21/WAF1 , and p27/KIP1, and inhibited the activation of NFkappaB and its gene products such as cyclin D1, VEGF, uPA, MMP-2, MMP-9, Bcl-2 and Bcl-XL ... In xenogrfated tumors, curcumin upregulated the expression of TRAIL-R1/DR4, TRAIL-R2/DR5, Bax, Bak, p21/WAF1, and p27/KIP1 , and inhibited the activation of NFkappaB and its gene products such as cyclin D1, VEGF, uPA, MMP-2, MMP-9, Bcl-2 and Bcl-XL ... In xenogrfated tumors, curcumin upregulated the expression of TRAIL-R1/DR4, TRAIL-R2/DR5 , Bax, Bak, p21/WAF1, and p27/KIP1, and inhibited the activation of NFkappaB and its gene products such as cyclin D1, VEGF, uPA, MMP-2, MMP-9, Bcl-2 and Bcl-XL ... In xenogrfated tumors, curcumin upregulated the expression of TRAIL-R1/DR4, TRAIL-R2/DR5, Bax , Bak, p21/WAF1, and p27/KIP1, and inhibited the activation of NFkappaB and its gene products such as cyclin D1, VEGF, uPA, MMP-2, MMP-9, Bcl-2 and Bcl-XL
Qiao et al., Mol Cell Biol 2008 : T-cell receptor induced NF-kappaB activation is negatively regulated by E3 ubiquitin ligase Cbl-b ... In this study, we report that the loss of Cbl-b selectively results in aberrant activation of NF-kappaB upon T-cell antigen receptor ( TCR ) ligation, which is mediated by phosphatidylinositol 3-kinase (PI3-K)/Akt and protein kinase C-theta (PKC-theta)
Khan et al., Infect Immun 2008 (Enterobacteriaceae Infections...) : These responses were accompanied by mitogen activated protein kinase activation, as well as the Toll-like receptor 5 (TLR5) dependent activation of NF-kappaB ... These responses were accompanied by mitogen activated protein kinase activation, as well as the Toll-like receptor 5 (TLR5) dependent activation of NF-kappaB
Yu et al., Yao Xue Xue Bao 2007 : Among the factors involved in this step, the cellular protein nuclear factor NF-kappaB is the most powerful inducer of HIV-1 transcription ... HIV-1 transcription is initiated by the binding of NF-kappaB to the enhancer region in the long terminal repeat ( LTR ) of HIV-1
Guasparri et al., Blood 2008 (Cell Transformation, Viral...) : EBV LMP2A affects LMP1 mediated NF-kappaB signaling and survival of lymphoma cells by regulating TRAF2 expression ... EBV LMP2A affects LMP1 mediated NF-kappaB signaling and survival of lymphoma cells by regulating TRAF2 expression ... We found that suppression of LMP1 by RNA interference results in inhibition of basal NF-kappaB and induction of apoptosis
Rosenwald et al., Hum Pathol 2008 (Hodgkin Disease) : An increase in eIF-4E expression may lead to constitutively high expression of NFkappaB , a transcription factor implicated in resistance to apoptosis and induction of cytokine gene expression in various cells, including neoplastic cells of HL
Gangoiti et al., Cell Signal 2008 : C1P stimulated the activity of NF-kappaB , and inhibitors of this transcription factor completely blocked macrophage proliferation
Chen et al., Cell Signal 2008 : In this study, we identified syntenin as a negative regulator of IL-1R and TLR4 mediated NF-kappaB activation ... Overexpressed syntenin inhibited IL-1- or LPS-, but not TNF- induced NF-kappaB activation and IL-8 mRNA expression in a dose dependent manner
Crisostomo et al., Am J Physiol Cell Physiol 2008 : VEGF , FGF2, and HGF expression was significantly reduced by NF kappa B inhibition ( 50 % decrease ) but not ERK or JNK inhibition ... VEGF, FGF2 , and HGF expression was significantly reduced by NF kappa B inhibition ( 50 % decrease ) but not ERK or JNK inhibition ... VEGF, FGF2 , and HGF expression was significantly reduced by NF kappa B inhibition ( 50 % decrease ) but not ERK or JNK inhibition ... VEGF, FGF2, and HGF expression was significantly reduced by NF kappa B inhibition ( 50 % decrease ) but not ERK or JNK inhibition ... Moreover, ERK, JNK, and NF kappa B inhibitor alone did not activate MSC VEGF expression over controls
Kimura et al., Invest Ophthalmol Vis Sci 2008 : TNF-alpha induced the phosphorylation and downregulation of IkappaB-alpha and the translocation of the p65 subunit of NF-kappaB to the nucleus
Sanz et al., J Am Soc Nephrol 2008 (Nephritis, Interstitial) : In vitro, it was found that TWEAK induced the sustained activation of NF-kappaB in a murine tubular epithelial cell line ( MCT ) ... Parthenolide, which prevents IkappaB-alpha degradation, inhibited TWEAK induced NF-kappaB activation and prevented the aforementioned changes in vitro and in vivo
Xu et al., Respir Res 2008 (Haemophilus Infections...) : In addition, the role of p38 MAPK and NF-kappa B on the NTHi induced COX-2 and PGE2 expression was investigated by using their specific chemical inhibitors ... Moreover, the expressions of COX-2 and PGE2 were significantly inhibited by specific inhibitors of p38 MAPK and NF-kappa B ... However, NTHi induced DNA binding activity of NF-kappa B was not affected by the inhibition of p38 MAPK
Nishida et al., Am J Physiol Gastrointest Liver Physiol 2008 : Furthermore, LY294002 suppressed both IL-1beta- and TNF-alpha induced NF-kappaB activation and IL-1beta-, TNF-alpha-, and IFN-gamma induced activated protein-1 (AP-1) activation
Iha et al., EMBO J 2008 (Heart Diseases...) : Here, we show that Tax1 binding protein 1 (TAX1BP1) is a negative regulator of TNF-alpha- and IL-1beta induced NF-kappaB activation and that binding to mono- and polyubiquitin by a ubiquitin binding Zn finger domain in TAX1BP1 is needed for TRAF6 association and NF-kappaB inhibition
Cho et al., FEBS Lett 2008 (Neoplasms) : Downregulation of lzts2 increased the beta-catenin/Tcf promoter activity and inhibited NF-kappaB induced modulation of the nuclear translocation of beta-catenin
Kaspari et al., FEBS Lett 2008 (Cytomegalovirus Infections) : Furthermore, in contrast to previous hypotheses we demonstrated that neither the ND10 components PML and hDaxx nor NFkappaB activation represent the target for MG132 ... Furthermore, in contrast to previous hypotheses we demonstrated that neither the ND10 components PML and hDaxx nor NFkappaB activation represent the target for MG132
Koga et al., FEBS Lett 2008 (Arthritis, Rheumatoid) : Inhibitor studies have shown SAA induced IL-6 production to be down-regulated by NF-kappaB inhibition and partially inhibited by p38 or JNK inhibitors
Zhao et al., Biochem Pharmacol 2008 : The pharmacological inhibitor of phosphatidylinositol 3-kinase (PI3K) ( wortmannin ) and dominant negative forms of p85 ( the regulatory subunit of PI3K ) or Akt enhance, while constitutive active forms of p110 ( the catalytic subunit of PI3K ) or Akt inhibit , NF-kappaB activation and the target gene interleukin (IL)-8 induced by MDP
Molnár et al., Immunol Lett 2008 : CR1/2 ligation by 7G6 scFv alone can induce nuclear translocation of NF-kappaB , supporting the above observations
Sors et al., Clin Cancer Res 2008 (Lymphoma, T-Cell, Cutaneous...) : Specific IKK2 inhibition resulting from transfection or from incubation with AS602868 allowed a down-regulation of NF-kappaB transcriptional activity
Duncan et al., Mol Cancer Ther 2008 : Additionally, we reported previously that BCR-ABL activates the transcription factor nuclear factor-kappaB (NF-kappaB) in a manner dependent on Ras and that inhibition of NF-kappaB by expression of a modified form of IkappaBalpha blocked BCR-ABL-driven tumor growth in a xenograft model ... Additionally, we reported previously that BCR-ABL activates the transcription factor nuclear factor-kappaB (NF-kappaB) in a manner dependent on Ras and that inhibition of NF-kappaB by expression of a modified form of IkappaBalpha blocked BCR-ABL-driven tumor growth in a xenograft model ... These data indicate that blockage of BCR-ABL induced NF-kappaB activation via IkappaB kinase beta inhibition represents a potential new approach for treatment of Imatinib- or Dasatinib-resistant forms of chronic myelogenous leukemia ... These data indicate that blockage of BCR-ABL induced NF-kappaB activation via IkappaB kinase beta inhibition represents a potential new approach for treatment of Imatinib- or Dasatinib-resistant forms of chronic myelogenous leukemia
D'Angelo et al., J Cell Physiol 2008 : The failure of the mycERtrade mark transgene to cause the apoptotic response is associated with reduced levels of RIP1 expression, increased Mcl-1 expression and activation of both NF-kB and Akt
Sodhi et al., Cytokine 2008 : It is further shown that p38 MAP kinase, STAT3 and NF-kappaB could play a differential regulatory role in PRL or GH induced production of cytokines by macrophages
Kao et al., Cell Signal 2008 : By using mutation constructs of luciferase reporter driven by Glut1 promoter/enhancers, chromatin immunoprecipitation and co-immunoprecipitation experiments, we were able to demonstrate that cooperative interaction between NF-kappaB and Sp1 were required to enhance Glut1 transcription in response to ET-1
Kim et al., J Immunol 2008 (MAP Kinase Signaling System...) : In this study, the effect of Lactobacillus plantarum lipoteichoic acid ( pLTA ) on LPS induced MAPK activation, NF-kappaB activation , and the expression of TNF-alpha and IL-1R associated kinase M (IRAK-M) was examined ... In this study, the effect of Lactobacillus plantarum lipoteichoic acid ( pLTA ) on LPS induced MAPK activation, NF-kappaB activation , and the expression of TNF-alpha and IL-1R associated kinase M (IRAK-M) was examined ... In this study, the effect of Lactobacillus plantarum lipoteichoic acid ( pLTA ) on LPS induced MAPK activation, NF-kappaB activation , and the expression of TNF-alpha and IL-1R associated kinase M (IRAK-M) was examined
Yoshitake et al., Nitric Oxide 2008 : Here, we investigated the effect of EDCs on lipopolysaccharide (LPS) induced NO production and NF-kappaB activation in the RAW264.7 mouse macrophage cell line ... ELISA based quantification of the DNA binding activity of free p65 NF-kappaB showed that LPS induced NF-kappaB activation was significantly diminished by EDC treatment
Bhattacharyya et al., J Biol Chem 2008 (Inflammation) : The sulfated polysaccharide carrageenan (CGN) induces activation of NFkappaB and interleukin 8 (IL-8) in human colonic epithelial cells through a pathway of innate immunity mediated by Bcl10 ( B-cell CLL/lymphoma 10 )
Reimer et al., J Leukoc Biol 2008 : poly ( I:C ) and LPS induce distinct IRF3 and NF-kappaB signaling during type-I IFN and TNF responses in human macrophages
Altman et al., Vascul Pharmacol 2008 (Inflammation) : Treatment of endothelial cells with DHEAS dramatically inhibited the TNF-alpha induced activation of NF-kappaB , an inflammatory transcription factor, and increased protein levels of the NF-kappaB inhibitor, IkappaB-alpha
Nonomura et al., Hinyokika Kiyo 2008 (Neoplasms, Hormone-Dependent...) : Besides adrenal androgen blockade, dexamethasone suppresses the growth of prostate cancer via NFkappaB inactivation, and also via the inhibition of interleukin-6 production which is reportedly important for the growth of prostate cancer
Hu et al., Biochem J 2008 : Existence of the canonical IKK2 [ IkappaB ( inhibitor of NF-kappaB ) kinase 2 ] /IkappaBalpha pathway of NF-kappaB activation induced by IL-1beta in rabbit colonic muscle cells was validated with multiple approaches, including the induction of reporter luciferase activity and endogenous NF-kappaB-target gene expression, NF-kappaB-DNA binding activity, p65 nuclear translocation, IkappaBalpha degradation and the phosphorylation of IKK2 at Ser ( 177/181 ) and p65 at Ser ( 536 ) ... RGS4 up-regulation by IL-1beta was blocked by selective inhibitors of IKK2, IkappaBalpha or NF-kappaB activation, by effective siRNA ( small interfering RNA ) of IKK2, and in cells expressing either the kinase-inactive IKK2 mutant ( K44A ) or the phosphorylation-deficient IkappaBalpha mutant ( S32A/S36A )
Vakkila et al., Scand J Immunol 2008 : By contrast, PGE2 supplemented DC could be characterized by negligible responses to LPS and IFN-gamma and a remarkable NF-kappaB response to CD40L ... By contrast, PGE2 supplemented DC could be characterized by negligible responses to LPS and IFN-gamma and a remarkable NF-kappaB response to CD40L ... By contrast, PGE2 supplemented DC could be characterized by negligible responses to LPS and IFN-gamma and a remarkable NF-kappaB response to CD40L
Yu et al., Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Société canadienne des sciences pharmaceutiques 2007 (Pneumonia...) : Ketamine at sub-anesthetic doses could suppress the production of inflammatory cytokines such as TNF-alpha and IL-6, attenuate NF-kappaB activity, and inhibit TLR2 and TLR4 expression in polymicrobial sepsis ... Ketamine at sub-anesthetic doses could suppress the production of inflammatory cytokines such as TNF-alpha and IL-6, attenuate NF-kappaB activity, and inhibit TLR2 and TLR4 expression in polymicrobial sepsis
Kim et al., Biochem Biophys Res Commun 2008 : Moreover, NSA9 suppressed the LPS induced nuclear translocation and activation of nuclear factor-kappaB (NF-kappaB)
Rebeaud et al., Nat Immunol 2008 : In contrast, MALT1 activity but not Bcl-10 cleavage was essential for optimal activation of transcription factor NF-kappaB and production of interleukin 2
Sonoki et al., Br J Pharmacol 2008 : MCP-1 mRNA expression and NF-kappaB activity were enhanced by venom sPLA2 treated LDL , which was completely suppressed by indoxam but not by thioetheramide-PC, a competitive sPLA2 inhibitor
van Beijnum et al., Angiogenesis 2008 (Neovascularization, Pathologic) : Furthermore, HMGB1 induced signaling can activate NFkappaB , which can subsequently induce the expression of HMGB1 receptors
Kotla et al., Virology 2008 : The type I interferon ( IFN ) response requires the coordinated activation of the latent transcription factors NF-kappaB , IRF-3 and ATF-2 which in turn activate transcription from the IFN-beta promoter
Boersma et al., Science signaling 2008 : Transcriptional activation by NF-kappaB and chromatin remodeling by inhibitor of kappaB (IkappaB) kinase complex ( IKK ) have been linked to recall and reconsolidation of conditioned fear memories in the mammalian central nervous system
Lee et al., Hepatology 2008 (Hepatitis C) : The activation of NF-kappaB was mediated by HCV-E2 induced expression of the molecular chaperone glucose regulated protein 94 (GRP94)
Bischoff et al., J Cell Biochem 2008 : NF-kappaB inhibition resulted in a decrease in CXCL8 levels in hMSCs grown in non-OGM
Erlejman et al., Arch Biochem Biophys 2008 : Hexameric procyanidins inhibit TNFalpha induced NF-kappaB activation in Caco-2 cells ... We investigated the capacity of a hexameric procyanidin fraction ( Hex ) to prevent tumor necrosis factor alpha (TNFalpha) induced NF-kappaB activation as related to oxidation and membrane interactions ... In Caco-2 cells, Hex ( 2.5-20 microM ) inhibited TNFalpha induced NF-kappaB activation ( IkappaB phosphorylation and degradation, p50 and RelA nuclear translocation, and NF-kappaB-DNA binding ), inducible nitric oxide synthase expression, and cell oxidant increase
Lee et al., J Biol Chem 2008 : TLR9 signaling also leads to the activation of NFkappaB , including the p65RelA subunit in wild type B cells ... The lack of Btk signaling affects the activation of NFkappaB and impairs the translocation of the p65RelA subunit to the nucleus of B cells upon TLR9 stimulation ... Thus, Btk plays an important role in TLR9 signaling and acts separately to regulate NFkappaB RelA activation as well as IL-10 and IL-12 production in B cells
Ohno et al., Oral Microbiol Immunol 2008 (Inflammation) : The NF-kappaB inhibitor suppressed the expression of CCL5 and MMP9, but not that of CXCL10, whereas P. gingivalis infection induced significant CCL5 expression in MyD88 ( -/- ) osteoblasts ... The NF-kappaB inhibitor suppressed the expression of CCL5 and MMP9 , but not that of CXCL10, whereas P. gingivalis infection induced significant CCL5 expression in MyD88 ( -/- ) osteoblasts
Fitzpatrick et al., Br J Nutr 2008 (Colitis) : EC-M17 dose dependently inhibited TNF-alpha induced NF-kappaB signalling
Yun et al., Int Immunopharmacol 2008 (MAP Kinase Signaling System) : Furthermore, asiatic acid inhibited the NF-kappaB activation induced by LPS , and this was associated with the abrogation of I kappa B-alpha degradation and with subsequent decreases in nuclear p65 and p50 protein levels
Youn et al., Int Immunopharmacol 2008 : Selenium inhibited NF-kappaB activation induced by poly [ I:C ] ( TLR3 agonist ), LPS ( TLR4 agonist ) or overexpression of MyD88 or IKK-beta which is the key kinase of MyD88 dependent signaling pathway ... Selenium inhibited NF-kappaB activation induced by poly [ I:C ] ( TLR3 agonist ), LPS ( TLR4 agonist ) or overexpression of MyD88 or IKK-beta which is the key kinase of MyD88 dependent signaling pathway ... Selenium inhibited NF-kappaB activation induced by poly [ I:C ] ( TLR3 agonist ), LPS ( TLR4 agonist ) or overexpression of MyD88 or IKK-beta which is the key kinase of MyD88 dependent signaling pathway
Wang et al., Biochem Biophys Res Commun 2008 : URP2SF , a FERM and PH domain containing protein, regulates NF-kappaB and apoptosis ... Over-expression of URP2SF strongly repressed NF-kappaB activity in the absence or presence of NF-kappaB stimulus ... Furthermore, suppression of URP2SF expression by small interference RNA significantly activated the NF-kappaB reporter gene activities, as well as attenuated TNF-alpha induced apoptosis
Kiningham et al., Free Radic Biol Med 2008 (Neuroblastoma) : All-trans-retinoic acid induces manganese superoxide dismutase in human neuroblastoma through NF-kappaB
Gartner et al., Vaccine 2008 : Transient transfection showed that the mutated caspase induced slow apoptosis, normal protein expression and NF-kappaB activation while wild-type caspase induced rapid apoptosis, lower protein expression and no NF-kappaB activation
Klinke et al., Biophys J 2008 : DCPA treatment resulted in a potentiation of early LPS induced NF-kappaB activation
Nah et al., Rheumatology (Oxford) 2008 (MAP Kinase Signaling System...) : In contrast, SN50, a nuclear factor-kappaB (NF-kappaB) inhibitor, had no effect on levels of COX-2 and PGE ( 2 )
Ulivi et al., J Cell Biochem 2008 (Inflammation) : p38/NF-kB dependent expression of COX-2 during differentiation and inflammatory response of chondrocytes ... In differentiated cells, the treatment with the inflammatory agent neither enhance NF-kB activation, nor synthesis of COX-2 while the addition of SB203580 neither repressed activation of p38, nor COX-2 synthesis, suggesting a constitutive activation of a p38/NF-kB/COX2 pathway
Mack et al., Proc Natl Acad Sci U S A 2008 : Here, we show that the murine CMV M45 protein binds to RIP1 and inhibits TNFalpha induced activation of NF-kappaB , p38 MAPK, and caspase independent cell death
Poole et al., J Virol 2008 (Cytomegalovirus Infections) : NF-kappaB mediated activation of the chemokine CCL22 by the product of the human cytomegalovirus gene UL144 escapes regulation by viral IE86 ... However, UL144 does activate NF-kappaB, resulting in NF-kappaB mediated activation of the cellular chemokine CCL22
Takada et al., Mol Pharmacol 2008 (Neoplasms) : We have shown previously that flavopiridol abrogates tumor necrosis factor (TNF) induced nuclear factor-kappaB (NF-kappaB) activation
Al-Lamki et al., J Am Soc Nephrol 2008 (Nephritis) : Inhibition of NF-kappaB activation increased TL1A mediated caspase-3 activation and apoptosis of TEC, but it did not reduce the induction of TNFR2 ... Inhibition of NF-kappaB activation increased TL1A mediated caspase-3 activation and apoptosis of TEC, but it did not reduce the induction of TNFR2
Lorz et al., J Am Soc Nephrol 2008 (Diabetic Nephropathies) : TRAIL also activated NF-kappaB , and inhibition of NF-kappaB sensitized cells to TRAIL induced apoptosis
MacLeod et al., Clinical and vaccine immunology : CVI 2008 : PorB induced NF-kappaB nuclear translocation was not dependent on either PTK or Erk1/2 activities ... PorB induced NF-kappaB nuclear translocation was not dependent on either PTK or Erk1/2 activities ... PorB induced NF-kappaB nuclear translocation was not dependent on either PTK or Erk1/2 activities
Choo et al., Oncol Rep 2008 (Colonic Neoplasms...) : SC-514, an inhibitor of IkappaB kinase beta (IKKbeta), blocked the TNF-alpha induced activation of nuclear factor-kappaB (NF-kappaB) as well as the TNF-alpha promoted metastasis of murine colon adenocarcinoma cells ... M1 was effective in suppressing the TNF-alpha induced activation of NF-kappaB , expression of matrix metalloprotease-9 (MMP-9), migration and invasion
Ogura et al., Exp Cell Res 2008 : Ectodomain shedding of TNF receptor 1 induced by protein synthesis inhibitors regulates TNF-alpha mediated activation of NF-kappaB and caspase-8 ... Nevertheless, we have surprisingly found that CHX, as well as its structural analogue acetoxycycloheximide ( Ac-CHX ), prevents TNF-alpha mediated activation of NF-kappaB and caspase-8 in human lung carcinoma A549 cells ... Thus, our results indicate that ectodomain shedding of TNF-R1 induced by protein synthesis inhibitors regulates TNF-alpha mediated activation of NF-kappaB and caspase-8
Waseem et al., Surgery 2008 : Exogenous ghrelin pretreatment resulted in a decrease in LPS induced NFkappaB activation and was presumably the reason for this ghrelin mediated effect
Ait-Ali et al., Endocrinology 2008 (Inflammation) : TNF-alpha , acting through the TNF-R2, causes an early up-regulation of NF-kappaB , long lasting induction of the NF-kappaB target gene inhibitor kappaB ( IkappaB ), and persistent stimulation of other NF-kappaB associated genes including mitogen-inducible gene-6 (MIG-6), which acts as an IkappaB signaling antagonist, and butyrate induced transcript 1
Zarnegar et al., Proc Natl Acad Sci U S A 2008 : Studies examining tumor-necrosis factor receptor associated factors ( TRAFs ) have identified positive roles for TRAF2, TRAF5 , and TRAF6, but not TRAF3, in canonical ( p50 dependent ) NF-kappaB activation ... Studies examining tumor-necrosis factor receptor associated factors ( TRAFs ) have identified positive roles for TRAF2, TRAF5, and TRAF6 , but not TRAF3, in canonical ( p50 dependent ) NF-kappaB activation ... Studies examining tumor-necrosis factor receptor associated factors ( TRAFs ) have identified positive roles for TRAF2, TRAF5, and TRAF6, but not TRAF3 , in canonical ( p50 dependent ) NF-kappaB activation ... In this article, we provide evidence that TRAF3 potently suppresses canonical NF-kappaB activation and gene expression in vitro and in vivo
Mollah et al., J Immunol 2008 (Diabetes Mellitus, Type 1...) : In TIDM monocytes, RelA and RelB were constitutively activated, and the src homology 2 domain containing protein tyrosine phosphatase (SHP-1) , a negative regulator of NF-kappaB , was overexpressed ... The monocyte and DC NF-kappaB response to LPS is thus a novel phenotypic and likely pathogenetic marker for human T1DM
Chuang et al., J Immunol 2008 : In contrast, IL-1beta- and TNF-alpha triggered NF-kappaB activation was not affected by DAPK
Küenzi et al., Apoptosis 2008 : Application of equal concentrations of MiliA to other cells such as the murine neuroblastoma cell line N2a resulted in immediate onset of apoptosis by nuclear translocation of apoptosis inducing factor ( AIF ), activation of caspases and c-Jun/AP-1 transcription factor without an intermediate differentiated phenotype, although minor transient phosphorylation of PKB and MAPK as well as activation of NF-kappaB were also observed ... Application of equal concentrations of MiliA to other cells such as the murine neuroblastoma cell line N2a resulted in immediate onset of apoptosis by nuclear translocation of apoptosis inducing factor ( AIF ), activation of caspases and c-Jun/AP-1 transcription factor without an intermediate differentiated phenotype, although minor transient phosphorylation of PKB and MAPK as well as activation of NF-kappaB were also observed
Kim et al., Eur J Pharmacol 2008 : Moreover, ILG attenuated the LPS induced DNA binding activity and the transcription activity of nuclear factor-kappa B (NF-kappaB) , and this was associated with a decrease in inhibitory kappa B-alpha ( IkappaB-alpha ) phosphorylation and in the subsequent blocking of p65 and p50 protein translocations to the nucleus
Tang et al., Proc Natl Acad Sci U S A 2008 : Here we show that NF-kappaB ( p65 ) represses myocardin activation of cardiac and smooth muscle genes in a CArG-box dependent manner
Yu et al., Br J Nutr 2008 (Atherosclerosis) : In this study, we investigated the effects of CA on TNF-alpha induced cell migration, the formation of intracellular reactive oxygen species, the translocation of NF-kappaB and the activation and expression of MMP-9 in human aortic smooth muscle cells ( HASMC ) ... In this study, we investigated the effects of CA on TNF-alpha induced cell migration, the formation of intracellular reactive oxygen species, the translocation of NF-kappaB and the activation and expression of MMP-9 in human aortic smooth muscle cells ( HASMC ) ... Furthermore, the production of reactive oxygen species and the nuclear translocation of NF-kappaB p50 and p65 induced by TNF-alpha were dose-dependently suppressed by CA pretreament
Lafuente et al., J Hypertens 2008 : However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB ... However, the high concentration of D-glucose did increase iNOS expression in response to low concentrations of IL-1beta ( 2.5 and 5 ng/ml ), as well as the IL-1beta induced activation of both ERK 1/2 and NF-kappaB
Xiang et al., Zhonghua Gan Zang Bing Za Zhi 2008 (Hepatitis B, Chronic) : Three kinds of immunological adjuvants, TNF alpha, CpG-ODN and CpG-ODN/HBsAg enhanced the expression of nucleic NF-kB and inhibited the expression of AP-1 in DC
Chen et al., Arterioscler Thromb Vasc Biol 2008 : Furthermore, STC1 partially blocked NF-kappaB and JNK activation in TNF-alpha treated endothelial cells ... JNK inhibitor and antioxidant also effectively blocked TNF-alpha induced NF-kappaB activation and monolayer permeability in HCAECs
Park et al., J Microbiol Biotechnol 2008 (Hypersensitivity, Immediate) : These extracts also inhibited mRNA expression of TNF-alpha and IL-4, as well as NF-kappaB activation in RBL-2H3 cells induced by the IgE-antigen complex ... These extracts also inhibited mRNA expression of TNF-alpha and IL-4 , as well as NF-kappaB activation in RBL-2H3 cells induced by the IgE-antigen complex
Kuntzen et al., Methods Mol Biol 2007 (Cell Transformation, Neoplastic) : Activation of NF-KB inhibits programmed cell death (PCD) triggered by tumor necrosis factor alpha (TNFalpha) and several other stimuli
Aoki et al., Biol Pharm Bull 2008 : These findings indicate that hepatocytes were stimulated directly by LPS, which lead to the activation of NF-kappaB and reduction of RXRalpha and HNF4alpha transcriptional activities as early responses, and indirectly by cytokines and NO released from KCs via activation of NF-kappaB by LPS as delayed responses ... We conclude that AG, which suppresses LPS induced NF-kappaB activation in both hepatocytes and KCs and then the release of cytokines and NO from KCs, attenuates LPS induced changes of Ntcp, Oatp1, Oatp4 and Mdr1b transcription in hepatocytes
van Galen et al., Histopathology 2008 (Lymphoma, Large B-Cell, Diffuse) : TRAF2 might be involved in activation of NF-kappaB in a subset of ABC-like DLBCL, and its expression is associated with a particularly poor outcome in primary nodal DLBCL patients
Cordier et al., J Mol Biol 2008 (Ectodermal Dysplasia, Hypohidrotic, Autosomal Recessive...) : This is sufficient to alter NEMO function, since functional complementation assays using NEMO-deficient pre-B and T lymphocytes show that full-length C417F pathogenic NEMO leads to a partial to strong defect in LPS, IL-1beta and TNF-alpha induced NF-kappaB activation, respectively, as compared to wild type NEMO ... This is sufficient to alter NEMO function, since functional complementation assays using NEMO-deficient pre-B and T lymphocytes show that full-length C417F pathogenic NEMO leads to a partial to strong defect in LPS, IL-1beta and TNF-alpha induced NF-kappaB activation, respectively, as compared to wild type NEMO
Kaler et al., Exp Cell Res 2008 (Colonic Neoplasms) : We showed that overexpression of HDAC2 enhanced TNFalpha induced NF-kappaB activity and that silencing of HDAC2 decreased NF-kappaB activity ... We showed that overexpression of HDAC2 enhanced TNFalpha induced NF-kappaB activity and that silencing of HDAC2 decreased NF-kappaB activity
Abraham et al., Virus Res 2008 : Further, induction of H-2Q4 and H-2T23 by JEV was independent of NF-kappaB but type 1 IFN dependent while H-2T10 was dependent on NF-kappaB and type 1 IFN independent
Jayakumar et al., Infect Immun 2008 : The inhibition of NF-kappaB activation by the pretreatment of DC with caffeic acid phenethyl ester blocks L. major induced IRF-1 and IRF-8 activation and IL-12 expression ... The inhibition of NF-kappaB activation by the pretreatment of DC with caffeic acid phenethyl ester blocks L. major induced IRF-1 and IRF-8 activation and IL-12 expression ... The inhibition of NF-kappaB activation by the pretreatment of DC with caffeic acid phenethyl ester blocks L. major induced IRF-1 and IRF-8 activation and IL-12 expression
Neil et al., Cancer Res 2008 (Disease Progression) : Expression of a truncated TAB1 mutant [ i.e., TAB1 ( 411 ) ] reduced basal and TGF-beta mediated NF-kappaB activation in NMuMG cells driven to undergo EMT by TGF-beta and in 4T1 cells stimulated by TGF-beta
Roccaro et al., Blood 2008 (Waldenstrom Macroglobulinemia) : We found that NPI-0052 inhibited proliferation and induced apoptosis in WM cells, and that the combination of NPI-0052 and bortezomib induced synergistic cytotoxicity in WM cells, leading to inhibition of nuclear translocation of p65NF-kappaB and synergistic induction of caspases-3, -8, and -9 and PARP cleavage
Chen et al., Shock 2008 (Reperfusion Injury) : Given that the preventive effect of LPS on I/R induced gut damage and NF-kappaB activity of the intestine is abolished in Tnfrsf1a mice, we conclude that TLR ligand decreases mesenteric I/R injury induced gut damage through TNF-alpha signaling
Sanchez-Ponce et al., Mol Cell Neurosci 2008 : We describe a role for IkappaB alpha , the NFkappaB inhibitor , in the initial stages of axon outgrowth and the development of the axon initial segment
Smith et al., J Clin Invest 1991 (Cell Transformation, Neoplastic...) : Tax also induces the nuclear expression of the NF-kappa B family of rel oncogene related enhancer binding proteins
Wang et al., American journal of physiology. Renal physiology 2008 (Diabetes Mellitus, Experimental...) : Furthermore, PEDF inhibited high glucose induced activation of NF-kappaB , a key transcription factor mediating inflammatory responses, and hypoxia-inducible factor-1, a major activator of VEGF expression in HMC
Marasa et al., Am J Physiol Cell Physiol 2008 : The present study tests the hypothesis that induced TRPC1 expression inhibits NF-kappaB activation by increasing PP2A activity through Ca2+ influx in IECs ... The present study tests the hypothesis that induced TRPC1 expression inhibits NF-kappaB activation by increasing PP2A activity through Ca2+ influx in IECs ... Inhibition of PP2A activity by treatment with okadaic acid or PP2A silencing with small interfering RNA not only enhanced NF-kappaB transactivation but also prevented the increased susceptibility of IEC-TRPC1 cells to apoptosis induced by treatment with tumor necrosis factor-alpha (TNF-alpha)/cycloheximide ( CHX ) ... Decreasing Ca2+ influx by exposure to the Ca2+-free medium reduced PP2A mRNA levels, destabilized PP2A proteins, and induced NF-kappaB activation, thus blocking the increased sensitivity of IEC-TRPC1 cells to TNF-alpha/CHX induced apoptosis
Torgler et al., J Immunol 2008 (Malaria) : Sporozoite mediated hepatocyte wounding limits Plasmodium parasite development via MyD88 mediated NF-kappa B activation and inducible NO synthase expression ... We provide further evidence that NF-kappaB activation causes the induction of inducible NO synthase expression in hepatocytes, and this is, in turn, responsible for a decrease in Plasmodium infected hepatocytes
Luyendyk et al., J Immunol 2008 (MAP Kinase Signaling System) : Modulating the PI3K-Akt pathway did not affect LPS induced degradation of IkappaBalpha or NF-kappaB nuclear translocation
Okamoto et al., J Rheumatol 2008 (Arthritis, Rheumatoid) : Nuclear factor-kappaB (NF-kappaB) activation by SAA was evaluated by luciferase assay ... Effects of preincubation of soluble RAGE on NF-kappaB activation by SAA was evaluated by Western blotting of IkappaBalpha ... SAA stimulated the transcriptional activation by NF-kappaB in a dose dependent manner and induced expression of the proinflammatory cytokines interleukin 6 (IL-6) and IL-8 ... SAA stimulation promoted nuclear translocation of NF-kappaB , whereas preincubation of SAA with RAGE inhibited nuclear translocation
Lenz et al., Science 2008 (Lymphoma, Large B-Cell, Diffuse) : In normal B cells, antigen receptor induced NF-kappaB activation requires CARD11 , a cytoplasmic scaffolding protein
Iwasaki et al., Endocr J 2008 : LPS also stimulated the expression of c-Fos gene and protein, and AP1-, but not NF-kappaB- , mediated transcription ... LPS also stimulated the expression of c-Fos gene and protein, and AP1-, but not NF-kappaB- , mediated transcription
Abboud et al., Shock 2008 (Anoxia...) : The addition of HSCM also attenuated LPS mediated NF-kappaB-DNA binding and IkappaBalpha degradation ... When HSCM and CM were subjected to Hsp72 depletion via adenosine triphosphate-agarose binding, LPS mediated activation of NF-kappaB was partially restored, suggesting that Hsp72 is partially responsible for cellular reprogramming in response to HSCM
Tahan et al., Clin Exp Allergy 2008 : TNF-alpha induced IL-8 release was suppressed by an NF-kappaB inhibitor but not by MEK or p38 MAPK inhibitors
Yang et al., Biochim Biophys Acta 2008 (Diabetic Nephropathies) : The aim of this study was to investigate whether high glucose induces aldose reductase ( AKR1B1 ) expression through NFkappaB , which may contribute to the pathogenesis of diabetic nephropathy ... These results show that NFkappaB induces AKR1B1expression under high glucose conditions, and the pattern of expression differs between nephropaths and the uncomplicated subjects
Kim et al., Biomed Pharmacother 2008 : Moreover, LPS induced NF-kappaB was diminished in macrophages subjected to prolonged treatment with low concentrations of curcumin ... Treatment with HO inhibitor abolished the inhibitory effect of curcumin on LPS induced NF-kappaB activation
Oliveira et al., J Biol Chem 2008 : The increase in TNFalpha mediated cell killing observed in hSMG-1 depleted cells is not related to the suppression of nonsense mediated mRNA decay or to the inhibition of TNFalpha induced NF-kappaB activation
Lee et al., J Biol Chem 2008 : The antibiotic promoted nuclear translocation of p65 and activation of NF-kappaB
Xiao et al., J Biol Chem 2008 : Pellino 3b negatively regulates interleukin-1 induced TAK1 dependent NF kappaB activation ... Pellino 3b negatively regulates interleukin-1 induced TAK1 dependent NF kappaB activation ... Pellino 3b negatively regulates interleukin-1 induced TAK1 dependent NF kappaB activation
Choi et al., Int Immunopharmacol 2008 : However, 15-MPA did not affect LPS induced degradation of inhibitor kappaB-alpha ( IkappaB-alpha ) and translocation of nuclear factor-kappaB (NF-kappaB) into the nucleus
Aimbire et al., Int Immunopharmacol 2008 (Inflammation) : LLLT and an inhibitor of NF-kappaB nuclear translocation ( BMS 205820 ) attenuated the mRNA levels of Bcl-xL and A1 mRNA in lung neutrophils obtained from mice subjected to LPS induced inflammation
Liu et al., Eur J Pharmacol 2008 (Disease Models, Animal...) : These data suggest that p38MAPK signaling may be involved in the activation of NF-kappaB , and activation of p38MAPK signaling may be one of the mechanisms of acute lung injury ... These data suggest that p38MAPK signaling may be involved in the activation of NF-kappaB , and activation of p38MAPK signaling may be one of the mechanisms of acute lung injury
Seo et al., Neuroscience 2008 (Pain) : In conclusion, POMC mRNA expression in the arcuate nucleus of the hypothalamus was increased by inflammatory pain stimuli, in which pERK1/2, pCaMK-IIalpha and NFkappaB may play an important role in the expression of the hypothalamic POMC gene and beta-endorphin expression
Lee et al., J Cell Physiol 2008 : Catalase inhibited the effect of H2O2 on MAPKs and NF-kappaB
Iyer et al., Cell Microbiol 2008 (Leukemia, Myeloid) : L. reuteri downregulated nuclear factor-kappaB (NF-kappaB) dependent gene products that mediate cell proliferation ( Cox-2 , cyclin D1 ) and cell survival ( Bcl-2, Bcl-xL ) ... L. reuteri downregulated nuclear factor-kappaB (NF-kappaB) dependent gene products that mediate cell proliferation ( Cox-2, cyclin D1 ) and cell survival ( Bcl-2 , Bcl-xL ) ... L. reuteri suppressed TNF induced NF-kappaB activation, including NF-kappaB dependent reporter gene expression in a dose-and time dependent manner
Kim et al., Br J Pharmacol 2008 (Edema) : Anti-inflammatory effects of liquiritigenin as a consequence of the inhibition of NF-kappaB dependent iNOS and proinflammatory cytokines production ... Treatment of Raw264.7 cells with liquiritigenin caused inhibition of LPS induced NF-kappaB DNA binding activity, due to repression of I-kappaBalpha phosphorylation and degradation
Friedrichs et al., J Mol Med (Berl) 2008 (Crohn Disease...) : Thus, DLG5 likely acts in the regulation of NFkB activation or caspase activation as part of host defence mechanisms
Herbein et al., Curr HIV Res 2008 : Since tumor necrosis factor alpha (TNFalpha) activates NF-kappaB and AP-1, we investigated the role of exogenous Nef protein in TNFalpha stimulated U937 cells and primary macrophages ... We observed that exogenous Nef and TNFalpha synergistically activate NF-kappaB and AP-1 in U937 cells and primary macrophages resulting in enhanced stimulation of the HIV-1 long terminal repeat ( LTR ), and subsequently in enhanced viral replication in both chronically infected promonocytic U1 cells and acutely HIV-1 infected primary macrophages
Ibe et al., Cell Prolif 2008 (Anoxia) : SMC-PV proliferated more than SMC-PA and PAF plus hypoxia augmented NF-kappaB protein expression ... SMC-PV proliferated more than SMC-PA and PAF plus hypoxia augmented NF-kappaB protein expression
Travaglione et al., Infectious agents and cancer 2008 : CNF1 is receiving an increasing attention as a putative factor involved in transformation because of its ability to : ( i ) induce COX2 expression, an immediate-early gene over expressed in some type of cancers ; ( ii ) induce a long lasting activation of the transcription factor NF-kB , a largely accepted marker of tumor cells ; ( iii ) protect epithelial cells from apoptosis ; ( iv ) ensue the release of pro-inflammatory cytokines in epithelial and endothelial cells ; and ( v ) promote cellular motility
Lin et al., Toxicol Appl Pharmacol 2008 : Furthermore, the involvement of NF-kappaB in TNF-alpha induced MMP-9 production was consistent with that TNF-alpha stimulated degradation of IkappaB-alpha and translocation of NF-kappaB into the nucleus which were blocked by helenalin, but not by U0126 and SP600125, revealed by immunofluorescence staining ... Furthermore, the involvement of NF-kappaB in TNF-alpha induced MMP-9 production was consistent with that TNF-alpha stimulated degradation of IkappaB-alpha and translocation of NF-kappaB into the nucleus which were blocked by helenalin, but not by U0126 and SP600125, revealed by immunofluorescence staining ... The regulation of MMP-9 gene transcription by MAPKs and NF-kappaB was further confirmed by gene luciferase activity assay
Liang et al., Mol Hum Reprod 2008 : Since IL-1beta activates NFkappaB , AP-1 and C/EBP, we over expressed these transcription factors alone and in combination and found that only NFkappaB alone increased FP mRNA expression
Fenton et al., Int J Cancer 2008 (Disease Models, Animal...) : These data indicate that adiponectin acts on preneoplastic colon epithelial cells to regulate cell growth via 2 distinct pathways inhibiting leptin induced NF-kappaB dependent autocrine IL-6 production and trans-IL-6 signaling
Ghavami et al., J Leukoc Biol 2008 (MAP Kinase Signaling System) : RAGE small interfering RNA pretreatment abrogated the S100A8/A9 induced NF-kappaB activation ... Our data indicate that S100A8/A9 promoted cell growth occurs through RAGE signaling and activation of NF-kappaB
Lin et al., Rheumatol Int 2008 (Chondrosarcoma...) : Using chondrosarcoma cells stimulated with IL-1beta, the effects of GLN on the mRNA and protein levels of MMP-3, the activation of JNK, ERK, p38, NF-kappaB , and AP-1, the nuclear translocation of NF-kappaB/Rel family members, and PI3-kinase/Akt activation were studied ... Using chondrosarcoma cells stimulated with IL-1beta, the effects of GLN on the mRNA and protein levels of MMP-3 , the activation of JNK, ERK, p38, NF-kappaB , and AP-1, the nuclear translocation of NF-kappaB/Rel family members, and PI3-kinase/Akt activation were studied ... Using chondrosarcoma cells stimulated with IL-1beta, the effects of GLN on the mRNA and protein levels of MMP-3, the activation of JNK, ERK, p38, NF-kappaB , and AP-1, the nuclear translocation of NF-kappaB/Rel family members, and PI3-kinase/Akt activation were studied
Liu et al., Cancer Sci 2008 (Breast Neoplasms) : Furthermore, we found that daintain/AIF-1 overexpression up-regulated the expression of cyclin D1 and enhanced the transcriptional activity of nuclear factor-kappa B (NF-kappaB) , a regulator of cyclin D1 expression ... Furthermore, we found that daintain/AIF-1 overexpression up-regulated the expression of cyclin D1 and enhanced the transcriptional activity of nuclear factor-kappa B (NF-kappaB) , a regulator of cyclin D1 expression ... In contrast, the down-regulation of daintain/AIF-1 expression decreased cyclin D1 expression and inhibited the transcriptional activity of NF-kappaB ... In contrast, the down-regulation of daintain/AIF-1 expression decreased cyclin D1 expression and inhibited the transcriptional activity of NF-kappaB
Tirumurugaan et al., Respir Res 2008 (Bronchial Hyperreactivity) : Regulation of CD38 expression in HASM cells involves the transcription factor NF-kappaB , and glucocorticoids inhibit this expression through NF-kappaB dependent and -independent mechanisms ... The EMSA results and the cd38 promoter-reporter assays confirm the functional role of NF-kappaB , AP-1 and GREs in the cd38 promoter in the transcriptional regulation of CD38
Shi et al., Nat Immunol 2008 : Our results collectively indicate that TRIM30alpha negatively regulates TLR mediated NF-kappaB activation by targeting degradation of TAB2 and TAB3 by a ` feedback ' mechanism
Tai et al., Oncogene 2008 (MAP Kinase Signaling System...) : Axl promotes cell invasion by inducing MMP-9 activity through activation of NF-kappaB and Brg-1
Okayasu et al., Life Sci 2008 : PPARalpha activators upregulate eNOS activity and inhibit cytokine induced NF-kappaB activation through AMP activated protein kinase activation ... PPARalpha activators upregulate eNOS activity and inhibit cytokine induced NF-kappaB activation through AMP activated protein kinase activation
Dhawan et al., J Biol Chem 2008 (Melanoma...) : These results indicate that the LTbeta-R constitutively induces NF-kappaB activation, and this event may be associated with autonomous growth of melanoma cells
Stilo et al., J Cell Sci 2008 : A20 is a negative regulator of BCL10- and CARMA3 mediated activation of NF-kappaB
Logan et al., Cancer Chemother Pharmacol 2009 (Mucositis) : Both MTX and 5-FU administration caused histological evidence of tissue damage in the AT as well as changes in tissue expression of NF-kappaB and specific pro-inflammatory cytokines
Larsen et al., Cancer Lett 2008 (Breast Neoplasms) : Inhibition of HIF-1alpha, HIF-2alpha, NF-kappaB and C/EBPbeta using siRNA had no effect on hypoxia induced MIF secretion
Hisatsune et al., J Immunol 2008 : Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation ... Molecular characterization of Helicobacter pylori VacA induction of IL-8 in U937 cells reveals a prominent role for p38MAPK in activating transcription factor-2, cAMP response element binding protein, and NF-kappaB activation
Karlsson et al., Cells Tissues Organs 2008 (Osteoarthritis) : The effect of NFkappaB activation on HES5 transcription was studied using the NFkappaB inhibitor pyrrolidine dithiocarbamate
Huang et al., Biol Psychiatry 2009 (Genetic Predisposition to Disease...) : Since DRD2 expression is regulated by transcription factor NF-kappaB , we suspect that rs2734849 may indirectly affect dopamine D(2) receptor density
Morris et al., Biochem Biophys Res Commun 2008 : The regulation of COX-2 expression was independent of NF-kappaB activity
Koh et al., Biochem Biophys Res Commun 2008 : CD7 expression and galectin-1 induced apoptosis of immature thymocytes are directly regulated by NF-kappaB upon T-cell activation ... In this study, we showed that CD7 promoter activity was increased by NF-kappaB and that this activity was synergistic when Sp1 was co-expressed in the immature T-cell line L7 ... From these results, we concluded that the regulation of CD7 gene expression through NF-kappaB activation induced by TCR/CD28 might have significant implications for T-cell homeostasis
Lee et al., Oncol Rep 2008 (MAP Kinase Signaling System...) : The transactivation of TNF-alpha stimulated NF-kappaB , AP-1 and Sp-1 were inhibited by U0126 and SB203580 treatment
Ragheb et al., Biochem Biophys Res Commun 2008 (Disease Models, Animal...) : Interestingly, IkappaB-kinase beta (IKKbeta) dependent NF-kappaB was activated in hepatocytes from the fructose fed hamster as an indication for PKC activation
Lee et al., J Endod 2008 (Pulpitis) : LPS stimulated translocation of nuclear factor kappa B (NF-kappaB) into the nucleus, which was blocked by inhibitors of amino kinase terminal ( AKT, LY294002 ), extracellular signal regulated kinase 1/2 ( ERK 1/2, PD98059 ), p38 ( SB203580 ), and c-jun NH2-terminal kinase ( JNK, SP600125 ) or terrein
Bijli et al., J Biol Chem 2008 : Consistent with this, thrombin induced NF-kappaB activity and ICAM-1 expression were prevented by the expression of a kinase-defective mutant or RNA interference knockdown of Syk ... Analysis of the NF-kappaB pathway showed that Syk contributes to thrombin induced NF-kappaB activation by controlling its transactivation potential and that this response is associated with tyrosine phosphorylation of RelA/p65
Yang et al., J Biol Chem 2008 : Previous studies established that IFN activates NFkappaB through a classical NFkappaB pathway that results in IkappaBalpha degradation and formation of p50 containing NFkappaB complexes, as well as an alternative pathway that involves NFkappaB inducing kinase and TRAF2 , which results in the formation of p52 containing NFkappaB complexes
Li et al., Nat Immunol 2008 (Inflammation) : Here we demonstrate that CUE domain containing 2 (CUEDC2) interacted with IKKalpha and IKKbeta and repressed activation of the transcription factor NF-kappaB by decreasing phosphorylation and activation of IKK
Samanta et al., Oncogene 2008 (Burkitt Lymphoma) : Blocking of nuclear factor (NF)-kappaB by dominant negative IkappaB-alpha plasmid did not block IL-10 expression, whereas knockdown of IRF-3 by siRNA resulted in downregulation of IL-10 in EBER ( + ) BL cells
Ryu et al., Mol Cell Biochem 2008 (Virus Diseases) : Activation of NF-kappaB by alloferon through down-regulation of antioxidant proteins and IkappaBalpha
Ye et al., J Cell Mol Med 2008 : TNF-alpha induced increase in MLCK promoter activity was mediated by NF-kappaB activation
Gaultier et al., Blood 2008 (Inflammation) : The mechanism by which LRP-1 inhibits NF-kappaB activity involves down regulating cell-surface tumor necrosis factor receptor-1 (TNFR1) and thus, inhibition of autocrine TNFR1 initiated cell signaling
Souza et al., J Endocrinol 2008 : IL-1beta, tumour necrosis factor alpha (TNFalpha) and a combination of all three pro-inflammatory cytokines increased while IFNgamma alone did not affect NFkappaB activity and iNOS gene and protein expression ... Interestingly, the anti-inflammatory cytokines IL-4, IL-13 and IL-10 decreased IL-1beta stimulated NFkappaB activation and iNOS expression
Boncoeur et al., Am J Pathol 2008 : In this study, we evaluated the role of CFTR in the control of ubiquitin-proteasome activity and nuclear factor (NF)-kappaB/IkappaB-alpha signaling after lung oxidative stress ... Caspase-3 inhibition by Z-DQMD in CFTR-sufficient lung cells mimicked the response profile of increased proteasomal degradation and reduced NF-kappaB activity observed in CFTR-deficient lung cells exposed to oxidative stress
Condello et al., Brain Res 2008 (Neuroblastoma) : An additive effect of NGF was observed on the retinoic acid induced transglutaminase 2 expression and enzyme activity, and NF-kappaB activation ... We hypothesize that NF-kappaB activation was dependent on the transamidating activity related to high levels of TG2, and NGF enhanced NF-kappaB activation by a different, synergistically acting, pathway
Alvarez-Soria et al., Rheumatology (Oxford) 2008 (Osteoarthritis, Knee) : The effects of diacerein, celecoxib, diclofenac, meloxicam and indomethacin on prostaglandin ( PG ) E2 production, cyclo-oxygenase-2 (COX-2) protein expression, nitrite levels, presence of MMP-1 and -13, and activation of nuclear factor-kappaB (NF-kappaB) were studied on stimulated OA synoviocytes and chondrocytes ... The effects of diacerein, celecoxib, diclofenac, meloxicam and indomethacin on prostaglandin ( PG ) E2 production, cyclo-oxygenase-2 (COX-2) protein expression, nitrite levels, presence of MMP-1 and -13 , and activation of nuclear factor-kappaB (NF-kappaB) were studied on stimulated OA synoviocytes and chondrocytes ... The effects of diacerein, celecoxib, diclofenac, meloxicam and indomethacin on prostaglandin ( PG ) E2 production, cyclo-oxygenase-2 (COX-2) protein expression, nitrite levels, presence of MMP-1 and -13, and activation of nuclear factor-kappaB (NF-kappaB) were studied on stimulated OA synoviocytes and chondrocytes ... Diacerein and NSAIDs inhibited IL-1beta stimulated NF-kappaB activation in synoviocytes and chondrocytes except indomethacin in synoviocytes
Yao et al., Am J Physiol Lung Cell Mol Physiol 2008 (Pneumonia) : The mouse strain that was more susceptible to effects of CS showed augmented lung inflammatory cell influx, activation of NF-kappaB and p38 MAPK , and increased levels of matrix metalloproteinase-9 and NF-kappaB dependent proinflammatory cytokines compared with resistant mouse strains
Lindström et al., Mol Pharmacol 2008 : Short-term exposure to HDACi enhanced interleukin (IL)-1beta induced NF-kappaB activity as a result of potentiating IkappaB kinase (IKK)beta activity, thereby leading to persistent turnover of IkappaBalpha/epsilon proteins and prolonging NF-kappaB phosphorylation, nuclear localization, and DNA binding ... Short-term exposure to HDACi enhanced interleukin (IL)-1beta induced NF-kappaB activity as a result of potentiating IkappaB kinase (IKK)beta activity, thereby leading to persistent turnover of IkappaBalpha/epsilon proteins and prolonging NF-kappaB phosphorylation, nuclear localization, and DNA binding
Dolga et al., J Alzheimers Dis 2008 (Alzheimer Disease...) : It was previously shown that activation of TNF-R2 signaling, which includes phosphorylation of protein kinase B (PKB)/Akt and activation of nuclear factor-kappa B (NF-kappaB) , protects neurons against ischemic or excitotoxic insults ... It was previously shown that activation of TNF-R2 signaling, which includes phosphorylation of protein kinase B (PKB)/Akt and activation of nuclear factor-kappa B (NF-kappaB) , protects neurons against ischemic or excitotoxic insults
Lim et al., Int J Biochem Cell Biol 2008 : Since NF-kappaB p65 activation induced the decrease of Hsc70 level, the interaction of Ku70 with importin-alpha and nuclear translocation of Ku70 increased upon the activation of NF-kappaB p65 ... In the cells treated with cerulein as a physiological stimulus to activate NF-kappaB p65, nuclear translocation of Ku70 increased through NF-kappaB p65 mediated decrease of Hsc70 level
Zhou et al., Eur J Pharmacol 2008 (Edema) : Furthermore, 4-methoxyhonokiol inhibited LPS mediated nuclear factor-kappaB (NF-kappaB) activation via the prevention of inhibitor kappaB ( IkappaB ) phosphorylation and degradation
Mumbengegwi et al., Mol Cell Biol 2008 : The compartmentalized production of superoxide ( *O ( 2 ) ( - ) ) by endosomal NADPH oxidase is important in the redox dependent activation of NF-kappaB following interleukin 1beta (IL-1beta) stimulation
Lee et al., Cell Res 2008 : The inhibition of p38 MAPK and JNK/SAPK blocked NF-kappaB activation
Boycott et al., Glia 2008 : Regulation of both EAAT-1 and EAAT-2 is mediated by NF-kappaB , and this transcriptional regulator is also required for increased production of TNFalpha
Chen et al., Zhong Nan Da Xue Xue Bao Yi Xue Ban 2008 : The activation of NF-kappaB can increase the expression of p-gp and survivin, which may be part of the molecular mechanism of multi-drug resistance of K562
Gregory et al., Eur J Immunol 2008 : Importantly, induction of chemokine gene expression ( MIP-2/CXCL2 , MCP-1/CCL2, MIP-1alpha/CCL3, MIP-1beta/CCL4 ) by Leishmania is NF-kappaB dependent , which implies that p35 RelA/p50 dimers are able to activate transcription, despite the absence of a recognized transcriptional transactivation domain ... Importantly, induction of chemokine gene expression ( MIP-2/CXCL2 , MCP-1/CCL2, MIP-1alpha/CCL3, MIP-1beta/CCL4 ) by Leishmania is NF-kappaB dependent , which implies that p35 RelA/p50 dimers are able to activate transcription, despite the absence of a recognized transcriptional transactivation domain
Luo et al., Nephrol Dial Transplant 2008 (Acute Kidney Injury...) : MT downregulated the TNF-alpha mRNA level, and PDTC inhibited the increases in both NF-kappaB translocation and TNF-alpha mRNA
Prasad et al., Mol Med 2008 (Lymphoma, T-Cell, Cutaneous) : In HUT-78 ( T helper 0 [ Th ( 0 ) ] cell line ), we showed that a decrease in gene expression of interleukin-2 (IL-2) and IL-2 receptor alpha ( IL-2Ralpha ) were due to decreased activation of nuclear factor-kappaB (NF-kappaB) in zinc deficient cells
Kil et al., Phytother Res 2008 : In addition, AGR decreased TNF-alpha induced activation of NF-kappaB
Jawan et al., Toxicol Appl Pharmacol 2008 : Moreover, addition of PD98059, a MAPK kinase inhibitor, significantly suppressed the LPS induced NF-kappaB nuclear translocation and GM-CSF production, suggesting that the PPF attenuated GM-CSF production in hepatocytes may be attributed to its suppressive effect on MAPK/ERK signaling pathway
Eswarappa et al., Innate Immun 2008 : LPS induced iNOS mRNA and protein expression and NF-kappaB activation were also inhibited by folimycin
Li et al., J Leukoc Biol 2008 (Inflammation) : There was an equally diminished alpha-MSH suppression of LPS stimulated intracellular activation of NF-kappaB and p38 phosphorylation
Mandinova et al., EMBO J 2008 : Conversely, Notch activation protects keratinocytes against apoptosis through a mechanism that is not linked to Notch induced cell cycle withdrawal or NF-kappaB activation
Kanda et al., Eur J Immunol 2008 : All the three IL enhanced the basal and IL-1beta induced transcriptional activities of NF-kappaB , while IL-12 and IL-27 enhanced STAT3 and STAT1 activities, respectively
Al-Sadi et al., J Immunol 2008 : The IL-1beta induced increase in MLCK mRNA transcription and subsequent increase in MLCK protein expression and Caco-2 TJ permeability was mediated by activation of NF-kappaB ... Our findings also indicate that the IL-1beta induced increase in MLCK protein expression and Caco-2 TJ permeability was mediated by an NF-kappaB dependent increase in MLCK gene transcription
Huang et al., Am J Physiol Lung Cell Mol Physiol 2008 (Hypertension, Pulmonary...) : Hemodynamic data, the expression of inhibitory (I)-kappaBalpha , caveolin-1, and Tie2 ( a membrane protein ), activation of PY-STAT3 and NF-kappaB , and superoxide chemiluminescence were examined
Chatterjee et al., Curr Drug Targets 2008 : Moreover, in human umbilical vein endothelial cells, NF-kappaB has been also shown to regulate this enzyme which, in turn, regulates the gene/protein expression of platelet endothelial cell adhesion molecule , intercellular cell adhesion molecule and angiogenesis ... Moreover, in human umbilical vein endothelial cells, NF-kappaB has been also shown to regulate this enzyme which, in turn, regulates the gene/protein expression of platelet endothelial cell adhesion molecule , intercellular cell adhesion molecule and angiogenesis
van Uden et al., Biochem J 2008 : siRNA ( small interfering RNA ) studies for individual NF-kappaB members revealed differential effects on HIF-1alpha mRNA levels, indicating that NF-kappaB can regulate basal HIF-1alpha expression ... Finally, when endogenous NF-kappaB is induced by TNFalpha ( tumour necrosis factor alpha ) treatment, HIF-1alpha levels also change in an NF-kappaB dependent manner ... In conclusion, we find that NF-kappaB can regulate basal TNFalpha and, in certain circumstances, the hypoxia induced HIF-1alpha
Boissy et al., Leuk Res 2008 (Bone Resorption...) : A 3 h pulse with 25 nM bortezomib followed by a 3-day culture in its absence markedly inhibited osteoclast activity as evaluated through bone resorption, TRAcP release, and RANKL induced NF-kappaB translocation into nuclei, an event dependent on proteasomes and critical for osteoclast function
Chae et al., Biochem Biophys Res Commun 2008 : IRF-2 regulates NF-kappaB activity by modulating the subcellular localization of NF-kappaB ... While the nuclear recruitment of RelA by IRF-2 augments TNFalpha induced NF-kappaB dependent transcription, the N-terminal truncated mutant form of IRF-2 inhibits the nuclear localization of RelA, and thus interferes with NF-kappaB activation ... Furthermore, the knockdown of IRF-2 by IRF-2 siRNA attenuates TNFalpha induced NF-kappaB dependent transcription by inhibiting the nuclear localization of RelA ... Thus, these results show that IRF-2 regulates NF-kappaB activity via the modulation of NF-kappaB subcellular localization
Hu et al., Cell Signal 2008 (Burkitt Lymphoma...) : The increase in VEGF expression by LPA is mediated by the activation of c-Jun N-terminal Kinase (JNK) and transcription factor NFkappaB since blocking JNK or NFkappaB activation inhibited LPA induced VEGF expression ... The increase in VEGF expression by LPA is mediated by the activation of c-Jun N-terminal Kinase (JNK) and transcription factor NFkappaB since blocking JNK or NFkappaB activation inhibited LPA induced VEGF expression ... Knocking down expression of VEGFR1 and inhibiting activation of NFkappaB and JNK also blocked LPA induced protection against apoptosis
Voronov et al., Biochem Pharmacol 2008 : Benzo [ a ] pyrene inhibits osteoclastogenesis by affecting RANKL induced activation of NF-kappaB ... RAW264.7 cells ( a mouse macrophage cell line capable of differentiating into osteoclasts in the presence of RANKL ) were exposed to different concentrations of RANKL and BaP and the effect on NF-kappaB activation, nuclear translocation, as well as the effect of NF-kappaB inhibitors on BaP mediated CYP1B1 gene expression was measured ... The results demonstrated that BaP inhibited both RANKL induced NF-kappaB activation and nuclear translocation ... The results demonstrated that BaP inhibited both RANKL induced NF-kappaB activation and nuclear translocation ... At the same time, BaP induced CYP1B1 gene expression was inhibited by two NF-kappaB inhibitors in a dose dependent manner, demonstrating that NF-kappaB is involved in a BaP mediated signaling pathway
Li et al., Biochem J 2008 : c-Src has been shown to activate NF-kappaB ( nuclear factor kappaB ) following H/R ( hypoxia/reoxygenation ) by acting as a redox dependent IkappaBalpha ( inhibitory kappaB ) tyrosine kinase
Furuta et al., Eur J Immunol 2008 (Malaria) : NFkappaB/GFP activity was observed in TLR4/MD-2- but not in TLR2 expressing cells following stimulation with Prx
Ahn et al., Exp Dermatol 2008 : LPS upregulated the expression of TLR4 and MyD88 and induced NF-kappaB nuclear translocation B nuclear translocation in melanocytes ... LPS upregulated the expression of TLR4 and MyD88 and induced NF-kappaB nuclear translocation B nuclear translocation in melanocytes ... LPS upregulated the expression of TLR4 and MyD88 and induced NF-kappaB nuclear translocation B nuclear translocation in melanocytes
Stuhlmeier et al., J Biol Chem 2008 (MAP Kinase Signaling System...) : Analyses of virus induced signal cascades, utilizing chemical inhibitors of MAPK and overexpressing mutated IKK and IkappaB, revealed that the MAPK p38 as well as the transcription factor NF-kappaB are essential for virus induced activation of HAS1
Hickey et al., J Leukoc Biol 2008 : CyaA enhanced LPS induced phosphorylation of p38 MAPK and ERK in DC, and inhibitors of p38 MAPK, MEK, or NF-kappaB suppressed IL-10 production in response to LPS and CyaA ... CyaA enhanced LPS induced phosphorylation of p38 MAPK and ERK in DC, and inhibitors of p38 MAPK, MEK, or NF-kappaB suppressed IL-10 production in response to LPS and CyaA ... CyaA enhanced LPS induced phosphorylation of p38 MAPK and ERK in DC, and inhibitors of p38 MAPK, MEK, or NF-kappaB suppressed IL-10 production in response to LPS and CyaA ... CyaA enhanced LPS induced phosphorylation of p38 MAPK and ERK in DC, and inhibitors of p38 MAPK, MEK, or NF-kappaB suppressed IL-10 production in response to LPS and CyaA
Xiao et al., Microbiol Immunol 2008 : Finally, disrupted Cap67 did not show any inhibitory effect on the TLR9 mediated activation of NF-kappaB in a luciferase reporter assay with HEK293T cells, although the TLR4 mediated activation was suppressed
Ben-Othman et al., Mol Immunol 2008 (Leishmaniasis...) : This impairment was not due to MAPK deactivation as inhibition of ERK1/2 and p38MAPK , actually enhances the transcriptional activity of NF-kappaB in response to initial contact of Leishmania with the murine macrophagic cell line Raw264.7 ... This impairment was not due to MAPK deactivation as inhibition of ERK1/2 and p38MAPK, actually enhances the transcriptional activity of NF-kappaB in response to initial contact of Leishmania with the murine macrophagic cell line Raw264.7 ... This impairment was not due to MAPK deactivation as inhibition of ERK1/2 and p38MAPK , actually enhances the transcriptional activity of NF-kappaB in response to initial contact of Leishmania with the murine macrophagic cell line Raw264.7
Zhang et al., Transplantation 2008 : TNF receptor signaling was required for activation of NF-kappaB , but not for activation of activating protein 1 family members junD and Fra-1 in day 2 allografts
Chen et al., Oncogene 2008 (Ovarian Neoplasms) : Regulation of IKKbeta by miR-199a affects NF-kappaB activity in ovarian cancer cells ... Regulation of IKKbeta by miR-199a affects NF-kappaB activity in ovarian cancer cells
Logunov et al., Oncogene 2008 (Cell Transformation, Neoplastic...) : Cells incubated with media conditioned with different species of mycoplasma showed constitutive activation of NF-kappaB and reduced activation of p53 , common characteristics of the majority of human tumor cells, with M. arginini having the strongest effect among the species tested
Lee et al., Arch Pharm Res 2008 : Inhibitory effect of citral on NO production by suppression of iNOS expression and NF-kappa B activation in RAW264.7 cells
Dueñas et al., Cardiovasc Res 2008 (Atherosclerosis) : In TLR2 transfected human embryonic kidney 293 cells and human monocytes, oxidized low-density lipoproteins inhibited nuclear factor (NF)-kappaB-driven transcriptional activity and chemokine gene expression in response to TLR2 ligands
Hartupee et al., J Biol Chem 2008 : Interleukin 1alpha induced NFkappaB activation and chemokine mRNA stabilization diverge at IRAK1 ... Surprisingly, although TRAF6 is essential for NFkappaB activation, it is not required for IL-1alpha induced mRNA stabilization ... IRAK1 , which is recognized to function upstream of TRAF6, is required for both mRNA stabilization and activation of NFkappaB
Lopez-Granados et al., Hum Mutat 2008 (Ectodermal Dysplasia...) : EDI has also been associated with a single heterozygous mutation at position Ser32 of the NF-kappaB inhibitor IkappaBalpha, one of two phosphorylation sites that are essential for targeting IkappaBalpha for proteasomal degradation and hence for activation of NF-kappaB
Lee et al., Mol Cells 2008 : Acrolein inhibited NF-kappaB and IRF3 activation by LPS, but it did not inhibit NF-kappaB or IRF3 activation by MyD88, inhibitor kappaB kinase (IKK)beta , TRIF, or TNF-receptor associated factor family member associated NF-kappaB activator ( TANK) binding kinase 1 (TBK1 ) ... Acrolein inhibited NF-kappaB and IRF3 activation by LPS, but it did not inhibit NF-kappaB or IRF3 activation by MyD88, inhibitor kappaB kinase (IKK)beta, TRIF, or TNF-receptor associated factor family member associated NF-kappaB activator ( TANK) binding kinase 1 (TBK1 ) ... Acrolein inhibited NF-kappaB and IRF3 activation by LPS, but it did not inhibit NF-kappaB or IRF3 activation by MyD88 , inhibitor kappaB kinase (IKK)beta, TRIF, or TNF-receptor associated factor family member associated NF-kappaB activator ( TANK) binding kinase 1 (TBK1 )
Martín-Vilchez et al., Br J Pharmacol 2008 (Hepatitis C) : AM3 triggered nuclear translocation of NF-kappaB and phosphorylation of p38 mitogen activated protein kinase ... AM3 promoted NF-kappaB activation in a TLR-4 dependent manner, and blocking TLR-4 activity attenuated the enhanced expression of CD80, CD83 and CD86 induced by AM3
Chabane et al., Osteoarthritis Cartilage 2008 : In the present study, we determined the effect of trichostatin A (TSA) and butyric acid ( BA ), two histone deacetylase (HDAC) inhibitors, on NO and PGE ( 2 ) synthesis, inducible NO synthase (iNOS) and cyclooxygenase (COX)-2 expression, and nuclear factor (NF)-kappaB DNA binding activity, in interleukin-1beta (IL-1) stimulated human OA chondrocytes, and on IL-1 induced proteoglycan degradation in cartilage explants ... Finally, we demonstrate that the DNA binding activity of NF-kappaB , was induced by IL-1 , but was not affected by treatment with HDAC inhibitors
Singh et al., J Biol Chem 2008 (Inflammation) : COG112 significantly reduced nuclear translocation of NF-kappaB , when assessed by electromobility shift assay, immunoblotting, and immunofluorescence for p65
Xu et al., J Virol 2008 (Cell Transformation, Neoplastic) : The stimulation of IRF-4 by LMP-1 requires signaling from LMP-1 and involves cellular NF-kappaB
Hii et al., J Infect Dis 2008 : We showed that B. pseudomallei activates NF-kappaB via Toll-like receptor (TLR) 2, TLR4, and TLR5 but can also activate NF-kappaB and induce interleukin (IL)-8 without involving TLRs. TLR independent activation depends on a functional Bsa type III secretion system ( T3SS ) and requires internalization
Woo et al., J Neurochem 2008 : Inhibition of MMP-3 or -9 significantly suppressed the expression of iNOS and pro-inflammatory cytokines and the activities of NF-kappaB , AP-1, and MAPK in LPS stimulated microglia
Mokhtari et al., Diabetes 2008 : MEKK-1 mediates cytokine induced JNK- and NF-kappaB activation, and this event is necessary for iNOS expression and cell death
Busik et al., Diabetes 2008 (Diabetic Retinopathy...) : Exposure of HRECs to 25 mmol/l glucose did not stimulate endogenous ROS production, activation of nuclear factor-kappaB (NF-kappaB) , extracellular signal related kinase ( ERK ), p38 and Jun NH ( 2 ) -terminal kinase ( JNK ), tyrosine phosphorylation, interleukin (IL)-1beta, or tumor necrosis factor-alpha (TNF-alpha) production and only slightly affected apoptotic cell death pathways compared with normal glucose ( 5 mmol/l ) ... Exposure of HRECs to 25 mmol/l glucose did not stimulate endogenous ROS production, activation of nuclear factor-kappaB (NF-kappaB) , extracellular signal related kinase ( ERK ), p38 and Jun NH ( 2 ) -terminal kinase ( JNK ), tyrosine phosphorylation, interleukin (IL)-1beta , or tumor necrosis factor-alpha (TNF-alpha) production and only slightly affected apoptotic cell death pathways compared with normal glucose ( 5 mmol/l ) ... Exposure of HRECs to 25 mmol/l glucose did not stimulate endogenous ROS production, activation of nuclear factor-kappaB (NF-kappaB) , extracellular signal related kinase ( ERK ), p38 and Jun NH ( 2 ) -terminal kinase ( JNK ), tyrosine phosphorylation, interleukin (IL)-1beta , or tumor necrosis factor-alpha (TNF-alpha) production and only slightly affected apoptotic cell death pathways compared with normal glucose ( 5 mmol/l )
Mendoza et al., Apoptosis 2008 : Death receptor-4 (DR4) expression is regulated by transcription factor NF-kappaB in response to etoposide treatment ... Herein, we determined that transcription factor NF-kappaB plays a role in genotoxin induced DR4 expression ... Moreover, expression of the p65 subunit of NF-kappaB is sufficient to increase DR4 protein levels ... MEK kinase 1 (MEKK1) is a serine threonine kinase that is activated following etoposide treatment and activates NF-kappaB ... Taken together, NF-kappaB plays a role in up-regulation of DR4 following etoposide treatment
Goon Goh et al., Cell Signal 2008 : Overall these novel data demonstrate an IKK2 dependent, predominantly G-protein independent pathway involved in PAR-2 regulation of NFkappaB phosphorylation in keratinocytes
Ballaun et al., Biochem Biophys Res Commun 2008 : Transcription of the caspase-14 gene in human epidermal keratinocytes requires AP-1 and NFkappaB ... Our data reveal the basic organization of the human caspase-14 promoter and suggest an important role of AP-1 and NFkappaB in the transcriptional control of caspase-14
Csiszar et al., Am J Physiol Heart Circ Physiol 2008 (Inflammation) : Resveratrol also inhibited CSE induced NF-kappaB activation and inflammatory gene expression in CAECs
Sethu et al., J Immunol 2008 (Calcium Signaling...) : PLD1 is also required for NFkappaB and ERK1/2 activation in human monocytic cells ... In addition, the coupling of TNF-alpha to activation of the phosphorylation of ERK1/2 and the activation of NFkappaB were inhibited by pretreating cells with antisense to PLD1, but not to PLD2 ; thus, demonstrating a specific requirement for PLD1
Abrahams et al., J Immunol 2008 (Disease Models, Animal...) : Furthermore, in the presence of TLR6 , exposure to PDG resulted in trophoblast NF-kappaB activation and triggered these cells to secrete IL-8 and IL-6
Baritaki et al., J Immunol 2008 (Neoplasms) : NPI-0052 induced inhibition of NF-kappaB activity was involved in TRAIL sensitization as corroborated by the use of the NF-kappaB inhibitor dehydroxymethylepoxyquinomicin
Azran-Shaish et al., J Mol Med (Berl) 2008 (Leukemia, T-Cell) : On this ground, the present study suggests that such conditions may enhance the risk for ATL and TSP-HAM in HTLV-1 carriers by increasing the Tax induced NF-kappaB activation
Zemskova et al., J Biol Chem 2008 (Prostatic Neoplasms) : PIM1 in turn mediates docetaxel induced activation of NFkappaB transcriptional activity, and PIM1 depends in part on RELA/p65 proteins for its prosurvival effects
Rius et al., Nature 2008 (Anoxia...) : NF-kappaB activation is controlled by IkappaB kinases (IKK) , mainly IKK-beta, needed for phosphorylation induced degradation of IkappaB inhibitors in response to infection and inflammation ... Using in vitro systems, it was reported that HIF-1alpha activates NF-kappaB , that NF-kappaB controls HIF-1alpha transcription and that HIF-1alpha activation may be concurrent with inhibition of NF-kappaB ... Here we show, with the use of mice lacking IKK-beta in different cell types, that NF-kappaB is a critical transcriptional activator of HIF-1alpha and that basal NF-kappaB activity is required for HIF-1alpha protein accumulation under hypoxia in cultured cells and in the liver and brain of hypoxic animals
Boonkaewwan et al., J Agric Food Chem 2008 : It was previously demonstrated that stevioside attenuates NF-kappaB dependent TNF-alpha and IL-1beta synthesis in LPS stimulated monocytes ... It was previously demonstrated that stevioside attenuates NF-kappaB dependent TNF-alpha and IL-1beta synthesis in LPS stimulated monocytes
Droll et al., Biochem Biophys Res Commun 2008 : TNFalpha caused a rapid activation of NF-kappaB within 15min as evidenced by gel shift assay ( EMSA )
Lee et al., American journal of physiology. Renal physiology 2008 (Kidney Diseases...) : Sevoflurane caused nuclear translocation of SMAD3 and reduced the TNF-alpha induced nuclear translocation of NF-kappaB in primary cultures of proximal tubules from TGF-beta1+/+ but not in TGF-beta1+/- mice
Fong et al., J Bone Miner Res 2008 (Neoplasms) : Activation of AP-1 and NF-kappaB DNA-protein binding and MAPKs after TGF-beta1 treatment was shown, and TGF-beta1 induced IL-8 promoter activity was inhibited by the specific inhibitors of MAPK cascades
Peng et al., Surgery 2008 (Pancreatitis) : PKC-zeta induces nuclear translocation of NF-kappaB via ERK1/2 dependent mechanisms
Holden et al., Cell Signal 2008 : The PKC selective inhibitors, Ro31-8220, Gö6976, GF109203X and Gö6983, revealed no effect on TNFalpha induced NF-kappaB DNA binding and a similar lack of effect on serine 32/36 phosphorylated IkappaBalpha and the loss of total IkappaBalpha indicates that activation of the core IKK-IkappaBalpha-NF-kappaB cascade by TNFalpha does not involve PKC
Popovic et al., J Leukoc Biol 2008 : The thrombin induced CX3CL1 expression was NF-kappaB dependent , as shown by EMSA, ELISA, and by inhibition of the NF-kappaB signaling pathway by the IkappaB kinase inhibitor acety-11-keto-beta-boswellic acid or by transient overexpression of a transdominant negative form of IkappaBalpha
Zmijewski et al., Am J Respir Crit Care Med 2008 (Respiratory Distress Syndrome, Adult) : To examine involvement of the mitochondrial electron transport chain complex I on LPS mediated NF-kappaB activation in neutrophils and neutrophil dependent acute lung injury
Fan et al., Adv Exp Med Biol 2008 (Neoplasms) : Regulation of programmed cell death by NF-kappaB and its role in tumorigenesis and therapy ... NF-kappaB most commonly antagonizes PCD by activating the expression of antiapoptotic proteins and antioxidant molecules, but it can also promote PCD under certain conditions and in certain cell types ... Here, we review the role of NF-kappaB in apoptotic and necrotic PCD , the mechanisms involved, and how its activity in the cell death response impacts cancer development, progression, and therapy
Hung et al., Eur J Pharmacol 2008 : A further analysis indicated that lycopene attenuated TNF-alpha induced IkappaB phosphorylation, NF-kappaB expression, and NF-kappaB p65 translocation from cytosol to nucleus ... In line with this, TNF-alpha induced NF-kappaB-DNA but not AP1-DNA complexes formation was inhibited by lycopene, as determined by the electrophoretic mobility shift assay ( EMSA ) ... Taken together, we provided here the first evidence showing that lycopene is able to inhibit TNF-alpha induced NF-kappaB activation , ICAM-1 expression, and monocyte-endothelial interaction, suggesting an anti-inflammatory role of lycopene and possibly explaining in part why lycopene can prevent cardiovascular diseases
Kang et al., Am J Physiol Lung Cell Mol Physiol 2008 (MAP Kinase Signaling System) : The transcriptional and posttranscriptional regulation of CD38 expression involves signaling through MAPKs and requires activation of NF-kappaB and activator protein-1 (AP-1)
Zou et al., Am J Physiol Heart Circ Physiol 2008 (Disease Models, Animal...) : Recombinant HSC70 induced NF-kappaB activation and cytokine expression and depressed myocardial contractility in a TLR4 dependent manner ... Recombinant HSC70 induced NF-kappaB activation and cytokine expression and depressed myocardial contractility in a TLR4 dependent manner
Lu et al., Cell Physiol Biochem 2008 : Inhibition of AKT activity by a dominant negative AKT construct or by a PI3K inhibitor reduced active NF-kappaB and STAT3 expression without significantly altering the activity of the latter ... Direct activation of NF-kappaB also enhanced STAT3 expression, an effect abrogated by NF-kappaB inhibitor
Min et al., Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008 (MAP Kinase Signaling System...) : SNP induced the phosphorylation of p38 MAPK and extracellular regulated kinase (ERK), degradation and phosphorylation of IkappaB, and activation of NF-kappaB ... SNP induced the phosphorylation of p38 MAPK and extracellular regulated kinase (ERK) , degradation and phosphorylation of IkappaB, and activation of NF-kappaB
Ogura et al., Int Immunopharmacol 2008 : The protein synthesis inhibitor cycloheximide ( CHX ) and its structural derivative acetoxycycloheximide ( Ac-CHX ) have been recently shown to block the TNF-alpha induced activation of NF-kappaB via ectodomain shedding of TNF receptor 1 (TNF-R1) in human lung carcinoma A549 cells ... In this study, we show that ERK and p38 MAP kinase are involved in the downregulation of cell surface TNF-R1 upon exposure to Ac-CHX and the subsequent inhibition of TNF-alpha induced NF-kappaB activation ... In this study, we show that ERK and p38 MAP kinase are involved in the downregulation of cell surface TNF-R1 upon exposure to Ac-CHX and the subsequent inhibition of TNF-alpha induced NF-kappaB activation
Kuprash et al., Cancer Lett 2008 (Inflammation...) : TNF via TNFR1 axis induces NFkappaB , and may contribute to inflammation facilitated neoplasia
Rodríguez-Calvo et al., Diabetes 2008 : This effect was associated with the capacity of GW501516 to impede LPS induced NF-kappaB activation ... Because mitogen activated protein kinase-extracellular signal related kinase ( ERK ) 1/2 ( MEK1/2 ) is involved in LPS induced NF-kappaB activation in adipocytes, we explored whether PPARbeta/delta prevented NF-kappaB activation by inhibiting this pathway ... Because mitogen activated protein kinase-extracellular signal related kinase ( ERK ) 1/2 ( MEK1/2 ) is involved in LPS induced NF-kappaB activation in adipocytes, we explored whether PPARbeta/delta prevented NF-kappaB activation by inhibiting this pathway ... Because mitogen activated protein kinase-extracellular signal related kinase ( ERK ) 1/2 ( MEK1/2 ) is involved in LPS induced NF-kappaB activation in adipocytes, we explored whether PPARbeta/delta prevented NF-kappaB activation by inhibiting this pathway ... Because mitogen activated protein kinase-extracellular signal related kinase ( ERK ) 1/2 ( MEK1/2 ) is involved in LPS induced NF-kappaB activation in adipocytes, we explored whether PPARbeta/delta prevented NF-kappaB activation by inhibiting this pathway
Rabi et al., Mol Carcinog 2008 (Prostatic Neoplasms) : Betulinic acid suppresses constitutive and TNFalpha induced NF-kappaB activation and induces apoptosis in human prostate carcinoma PC-3 cells ... BetA mediated NF-kappaB inhibition involved decreased IKK activity and phosphorylation of IkappaBalpha at serine 32/36 followed by its degradation ... BetA mediated NF-kappaB inhibition involved decreased IKK activity and phosphorylation of IkappaBalpha at serine 32/36 followed by its degradation ... BetA also inhibited TNFalpha induced activation of NF-kappaB via the IkappaBalpha pathway, thereby sensitizing the cells to TNFalpha induced apoptosis
Yoon et al., Biochem Biophys Res Commun 2008 (Glioma) : Finally, we showed that inhibition of AMPK reduced DNA binding and transcriptional activation of nuclear factor-kappaB (NF-kappaB) in KA-treated cells
Kim et al., Exp Mol Med 2008 : Finally, pretreatment with pyrrolidine dithiocarbamate ( PDTC ), an inhibitor of NF-kappaB, reduced TNF-alpha induced ICAM-1 expression and both DPI and RacN17 significantly diminished NF-kappaB activation in response to TNF-alpha
Vaitaitis et al., PloS one 2008 : CD40 engagement of Th40 cells induces Nf-kappaB DNA binding activity and anti-apoptotic Bcl-X ( L ) expression in all three mouse strains
Jeong et al., Phytother Res 2008 : Furthermore, CAA inhibited the LPS induced DNA binding activity of nuclear factor-kappa B (NF-kappaB) and this effect was mediated through inhibiting the degradation of inhibitory factor-kappaBalpha ( IkappaBalpha ) ... Therefore, the results demonstrate that CAA inhibits LPS induced production of NO and expression of iNOS by blocking NF-kappaB activation
Rehman et al., J Biol Chem 2008 (Carcinoma, Squamous Cell...) : In this report we show that the NF-kappaB signaling pathway is activated in response to SDF-1alpha in HNSCC while primary and immortalized keratinocytes show no SDF-1alpha mediated NF-kappaB activity ... We found that SDF-1alpha mediated NF-kappaB signaling is independent of phosphoinositide 3-kinase/Akt and ERK/MAPK pathways ... We observed that SDF-1alpha induces IkappaBalpha phosphorylation and degradation and the nuclear translocation of NF-kappaB in HNSCC cell lines, suggesting that SDF-1alpha activates the classical NF-kappaB signaling pathway ... Contrary to previous reports, SDF-1alpha induced NF-kappaB activation is not mediated by tumor necrosis factor alpha ... Contrary to previous reports, SDF-1alpha induced NF-kappaB activation is not mediated by tumor necrosis factor alpha
Le Clorennec et al., J Virol 2008 : Therefore, our results are in agreement with a model where LMP1 dependent NF-kappaB activation induces Fas overexpression and autoactivation that could overwhelm the antiapoptotic effect of NF-kappaB, revealing an ambivalent function of LMP1 in cell survival and programmed cell death ... Therefore, our results are in agreement with a model where LMP1 dependent NF-kappaB activation induces Fas overexpression and autoactivation that could overwhelm the antiapoptotic effect of NF-kappaB, revealing an ambivalent function of LMP1 in cell survival and programmed cell death
Liao et al., Curr Biol 2008 : CARP-2 is an endosome associated ubiquitin ligase for RIP and regulates TNF induced NF-kappaB activation ... CARP-2 is an endosome associated ubiquitin ligase for RIP and regulates TNF induced NF-kappaB activation ... One important consequence of signaling is activation of the transcription factor NF-kappaB, and failure to downregulate TNF induced NF-kappaB transcriptional activity results in chronic inflammation and death ... We report that CARP-2, a RING domain containing ubiquitin protein ligase (E3), is a negative regulator of TNF induced NF-kappaB activation ... Knockdown of CARP-2 stabilized TNFR1 associated polyubiquitinated RIP levels after TNF simulation and enhanced activation of NF-kappaB ... CARP-2 acts at the level of endocytic vesicles to limit the intensity of TNF induced NF-kappaB activation by the regulated elimination of a necessary signaling component within the receptor complex
Funami et al., J Biol Chem 2008 : In this study, we show that homo-oligomerization of TICAM-1 is critical for TICAM-1 mediated activation of NF-kappaB and IRF-3
Shimizu et al., Microbiology 2008 : The Triton X-114 ( TX-114 ) detergent phase of U. parvum was found to induce NF-kappaB through TLR2
Liu et al., Cell Microbiol 2008 (Coxsackievirus Infections...) : However, FRNK transfection and phosphatidylinositol 3-kinase (PI3K) inhibitor LY294002 both blocked the IGTP induced translocation and NF-kappaB activation ... Furthermore, silencing NF-kappaB with siRNAs significantly inhibited the phosphorylation of FAK and Akt , but not their total expression levels, indicating that NF-kappaB activation is required for the IGTP induced activation of FAK and PI3K/Akt ... Furthermore, silencing NF-kappaB with siRNAs significantly inhibited the phosphorylation of FAK and Akt, but not their total expression levels, indicating that NF-kappaB activation is required for the IGTP induced activation of FAK and PI3K/Akt
Hsieh et al., Biochim Biophys Acta 2008 : Moreover, thrombin stimulated activation of NF-kappaB, AP-1 , and COX-2 promoter activity was blocked by the inhibitors of c-Src, PKC, EGFR, MEK1/2, AP-1 and NF-kappaB , suggesting that thrombin induces COX-2 promoter activity mediated through PKC ( delta ) /c-Src dependent EGFR transactivation, MEK-ERK1/2, AP-1, and NF-kappaB ... Moreover, thrombin stimulated activation of NF-kappaB , AP-1, and COX-2 promoter activity was blocked by the inhibitors of c-Src , PKC, EGFR, MEK1/2, AP-1 and NF-kappaB, suggesting that thrombin induces COX-2 promoter activity mediated through PKC ( delta ) /c-Src dependent EGFR transactivation, MEK-ERK1/2, AP-1, and NF-kappaB ... Moreover, thrombin stimulated activation of NF-kappaB , AP-1, and COX-2 promoter activity was blocked by the inhibitors of c-Src, PKC, EGFR , MEK1/2, AP-1 and NF-kappaB, suggesting that thrombin induces COX-2 promoter activity mediated through PKC ( delta ) /c-Src dependent EGFR transactivation, MEK-ERK1/2, AP-1, and NF-kappaB ... Moreover, thrombin stimulated activation of NF-kappaB , AP-1, and COX-2 promoter activity was blocked by the inhibitors of c-Src, PKC, EGFR, MEK1/2 , AP-1 and NF-kappaB, suggesting that thrombin induces COX-2 promoter activity mediated through PKC ( delta ) /c-Src dependent EGFR transactivation, MEK-ERK1/2, AP-1, and NF-kappaB ... Moreover, thrombin stimulated activation of NF-kappaB, AP-1, and COX-2 promoter activity was blocked by the inhibitors of c-Src, PKC, EGFR, MEK1/2, AP-1 and NF-kappaB , suggesting that thrombin induces COX-2 promoter activity mediated through PKC ( delta ) /c-Src dependent EGFR transactivation, MEK-ERK1/2, AP-1, and NF-kappaB
Bhattacharyya et al., Biochim Biophys Acta 2008 : The combination of ROS quenching by the free radical scavenger Tempol and of Bcl10 silencing by siRNA completely inhibited the CGN induced increases in nuclear NFkappaB ( p65 ), phospho-IkappaBalpha, and secretion of IL-8
Hattori et al., FEBS Lett 2008 : On the other hand, cells preincubated with HMW adiponectin had reduced TNFalpha induced NF-kappaB activation ... HMW adiponectin by itself was found to modestly activate NF-kappaB , which was significantly enhanced by inhibition of AMPK/eNOS activation ... HMW adiponectin by itself was found to modestly activate NF-kappaB , which was significantly enhanced by inhibition of AMPK/eNOS activation
Prickett et al., J Biol Chem 2008 : TAB4 mutated at Phe-Pro dominantly interfered with IL-1beta activation of NF-kappaB involving IKK dependent but not p38 MAPK dependent signaling
Hayes et al., Eur J Nutr 2008 (Neoplasms) : In particular, their abilities to induce cytoprotective genes, mediated by the Nrf2 ( NF-E2 related factor 2 ) and AhR ( arylhydrocarbon receptor ) transcription factors, and their abilities to repress NF-kappaB ( nuclear factor-kappaB ) activity, inhibit histone deacetylase , and inhibit cytochrome P450 are outlined
Bren et al., PloS one 2008 (Alstrom Syndrome) : Given the independent observations that acute T cell HIV-1 infection results in ( 1 ) NF-kappaB activation, ( 2 ) caspase 8 dependent apoptosis, and that ( 3 ) caspase 8 directly activates NF-kappaB , we questioned whether these three events might be interrelated ... Finally, we demonstrate that the HIV protease cleavage of caspase 8 is necessary for optimal NF-kappaB activation and that the HIV-1 protease specific cleavage fragment Casp8p41 is sufficient to stimulate HIV-1 replication through NF-kappaB dependent HIV-LTR activation both in vitro as well as in cells from HIV infected donors ... Finally, we demonstrate that the HIV protease cleavage of caspase 8 is necessary for optimal NF-kappaB activation and that the HIV-1 protease specific cleavage fragment Casp8p41 is sufficient to stimulate HIV-1 replication through NF-kappaB dependent HIV-LTR activation both in vitro as well as in cells from HIV infected donors ... Finally, we demonstrate that the HIV protease cleavage of caspase 8 is necessary for optimal NF-kappaB activation and that the HIV-1 protease specific cleavage fragment Casp8p41 is sufficient to stimulate HIV-1 replication through NF-kappaB dependent HIV-LTR activation both in vitro as well as in cells from HIV infected donors
Sur et al., Arch Dermatol Res 2008 (Dermatitis, Contact...) : Furthermore, cells treated with avenanthramides showed a significant inhibition of tumor necrosis factor-alpha (TNF-alpha) induced NF-kappaB luciferase activity and subsequent reduction of interleukin-8 (IL-8) release
Sun et al., J Biomed Sci 2008 (Carcinoma, Hepatocellular) : In summary, NF-kappaB activation is critically involved in the production of Wnt-1 by HepG2 cells
Young et al., J Invest Dermatol 2008 (Psoriasis...) : Employing a series of in vitro and in cellulo approaches, we have demonstrated that in primary human keratinocytes ( i ) TNF-alpha rapidly induces ROS generation, IkappaB degradation, NF-kappaB p65 nuclear translocation, and ultimately production of inflammatory cytokines ; ( ii ) TNF-alpha induced cytokine production is mediated both by the mammalian target of rapamycin signaling pathway via NF-kappaB activation and by ROS ; ( iii ) TNF-alpha dependent NF-kappaB activation ( that is, IkappaB degradation and NF-kappaB p65 nuclear translocation ) is not mediated by ROS ; and ( iv ) a cell penetrating derivative of the antioxidant enzyme, catalase, as well as taurine and N-acetyl-cysteine attenuate the TNF-alpha induced production of cytokines ... Employing a series of in vitro and in cellulo approaches, we have demonstrated that in primary human keratinocytes ( i ) TNF-alpha rapidly induces ROS generation, IkappaB degradation, NF-kappaB p65 nuclear translocation, and ultimately production of inflammatory cytokines ; ( ii ) TNF-alpha induced cytokine production is mediated both by the mammalian target of rapamycin signaling pathway via NF-kappaB activation and by ROS ; ( iii ) TNF-alpha dependent NF-kappaB activation ( that is, IkappaB degradation and NF-kappaB p65 nuclear translocation ) is not mediated by ROS ; and ( iv ) a cell penetrating derivative of the antioxidant enzyme, catalase, as well as taurine and N-acetyl-cysteine attenuate the TNF-alpha induced production of cytokines
Ortis et al., Diabetologia 2008 (Diabetes Mellitus, Type 1) : IL-1beta, however, induced a higher rate of expression of NF-kappaB target genes putatively involved in beta cell dysfunction and death and a stronger activation of the IKK complex , leading to an earlier translocation of NF-kappaB to the nucleus
Yin et al., Pediatr Transplant 2008 (Vascular Diseases) : Both LFO and HFO significantly decreased the CAV score, inhibited recruitment of T lymphocytes and macrophages, elevated the activity of PPAR gamma, inhibited the activity of NF-kappaB , reduced levels of intragraft MCP-1 and IP-10 as well as downregulated expression of chemokine receptors CCR2 ... Both LFO and HFO significantly decreased the CAV score, inhibited recruitment of T lymphocytes and macrophages, elevated the activity of PPAR gamma, inhibited the activity of NF-kappaB , reduced levels of intragraft MCP-1 and IP-10 as well as downregulated expression of chemokine receptors CCR2 ... Our results demonstrated that fish oil might attenuate CAV development, possibly through activating PPAR gamma and subsequently inhibiting the NF-kappaB activation, the chemokines secretion, as well as the CCR2 expression
Brechter et al., Bone 2008 : EMSA showed that IL-1beta and TNF-alpha activated NF-kappaB and AP-1 in MG-63 cells ... EMSA showed that IL-1beta and TNF-alpha activated NF-kappaB and AP-1 in MG-63 cells ... IL-1beta stimulated NF-kappaB via a non-canonical pathway ( p52/p65 ) and TNF-alpha via the canonical pathway ( p50/p65 ) ... p38 and JNK were important for TNF-alpha induced stimulation of B1 receptors, whereas NF-kappaB , p38 and JNK were involved in TNF-alpha induced expression of B2 receptors
Ye et al., Am J Physiol Heart Circ Physiol 2008 (Disease Models, Animal...) : We investigated 1 ) whether 3-day ATV pretreatment limits IS in eNOS ( -/- ) and iNOS ( -/- ) mice and 2 ) whether COX2 expression and/or activation by ATV is eNOS, iNOS, and/or NF-kappaB dependent ... In the iNOS ( -/- ) mice, eNOS and p-eNOS levels were increased but not iNOS and COX2 levels ; however, NF-kappaB was activated
Kang et al., Cardiovasc Res 2008 (Disease Models, Animal...) : Nuclear factor-kappa B (NF-kappaB) is a potent inducer of pro-inflammatory cytokines (PIC) and oxidative stress in cardiovascular disease
Matta et al., Oncogene 2008 (Sarcoma, Kaposi) : However, neither the IKKs nor NF-kappaB activation is required for nuclear localization of K13
Fantini et al., Curr Drug Targets 2008 (Cell Transformation, Neoplastic...) : Moreover, the activation of the nuclear factor kappa B (NF kappa B) , a transcription factor activated by several cytokines released during inflammation and responsible for many of their proinflammatory effects, have been shown to promote the growth of the colon tumors in experimental models
Hartz et al., FASEB J 2008 : Inhibition of JNK, but not NF-kappaB , blocked DEP induced P-glycoprotein up-regulation, indicating a role for AP-1 in the signaling pathway
Shimizu et al., Infect Immun 2008 : The Triton X-114 detergent phase of M. genitalium was found to induce NF-kappaB through TLR2 ... These results indicate that the activation of NF-kappaB by MG149 is dependent on TLR1 and TLR2 ... These results indicate that the activation of NF-kappaB by MG149 is dependent on TLR1 and TLR2 ... A synthetic lipopeptide derived from MG149 containing three acyl chains also induced NF-kappaB through TLR1 and TLR2 ... A synthetic lipopeptide derived from MG149 containing three acyl chains also induced NF-kappaB through TLR1 and TLR2
Kawauchi et al., Biochem Biophys Res Commun 2008 : Activated p53 induces NF-kappaB DNA binding but suppresses its transcriptional activation ... Thus, activated p53 may suppress transcriptional activity of NF-kappaB through inhibition of IKK and histone H3 kinase on DNA, suggesting a novel p53 mediated suppression system for tumorigenesis
Zhang et al., Zhonghua Zhong Liu Za Zhi 2007 (Neoplasm Invasiveness...) : [ Epidermal growth factor mediated NF-kappaB activation promotes uPA expression and invasiveness in pancreatic cancer cells ] ... Furthermore, EGF stimulated the NF-kappaB binding activity, and pretreatment of cells with a NF-kappaB inhibitor, pyrrolidine dithiocarbamate, markedly attenuated EGF induced NF-kappaB activation ... Subsequently, the EGF induced uPA expression and invasiveness were also inhibited by NF-kappaB inhibitor ... Our findings indicated that NF-kappaB mediated up-regulation of uPA expression is responsible for EGF induced invasiveness in pancreatic cancer cells, and implicate that such anti-NF-kappaB therapy with NF-kappaB inhibitors may contribute to the reduction of invasiveness of pancreatic cancer
Kato et al., Cell Immunol 2008 : In comparison to the control cells, introduction of PICOT to RBL-2H3 cells induced increased degranulation and the activation of NFAT and in the expression of IL-4 and TNF-alpha transcripts by FcepsilonRI crosslinking, whereas no significant change was observed with the elevation of ERK1/2 and p38 MAP kinase phosphorylation and NF-kappaB activation by FcepsilonRI aggregation
Wu et al., J Cell Physiol 2008 (Sepsis) : In conclusion, LPS + IFNgamma induced NFkappaB activation is necessary for iNOS induction but is not dependent on ROS signaling ... In conclusion, LPS + IFNgamma induced NFkappaB activation is necessary for iNOS induction but is not dependent on ROS signaling
Bowie et al., Biochem Soc Trans 2008 : Further, I demonstrate how understanding one molecular mechanism whereby vaccinia virus inhibits NF-kappaB activation has led to a revealing of a key role for IRAK-2 in TRAF-6 mediated NF-kappaB activation ... Further, I demonstrate how understanding one molecular mechanism whereby vaccinia virus inhibits NF-kappaB activation has led to a revealing of a key role for IRAK-2 in TRAF-6 mediated NF-kappaB activation
Lin et al., J Ethnopharmacol 2008 (Inflammation) : We further evaluated the effect of ASE on oxidative stress-sensitive transcription nuclear factor-kappa B (NF-kappaB) activation ... ASE also reduced DNA binding activity of NF-kappaB in LPS plus IFN-gamma stimulated RAW264.7 macrophages
Zhang et al., Int Immunopharmacol 2008 (Endotoxemia) : Further studies revealed that florfenicol prevented the LPS induced nuclear factor-kappaB (NF-kappaB) translocation from cytoplasm into nuclear in RAW 264.7 macrophages
Yang et al., Molecular cancer 2008 (Lymphoma, Mantle-Cell) : Consistent with a proteasome inhibitor resistant pathway of activation described in mouse B-lymphoma cells ( WEHI231 ) and a breast carcinoma cell line ( MDA-MB-468 ), the bortezomib-resistant NF-kappaB activity in Rec-1 cells is inhibited by calcium chelators, calmodulin inhibitors, and perillyl alcohol, a monoterpene capable of blocking L-type calcium channels
Wan et al., Biomed Pharmacother 2008 : These findings provide evidence for the involvement of the nuclear hormone receptors PPARgamma in the activity of NFkappaB in cerulein treated AR42J cells
Hagemann et al., J Exp Med 2008 (Neoplasms) : We show that macrophages are polarized via interleukin (IL)-1R and MyD88 to an immunosuppressive `` alternative '' phenotype that requires IkappaB kinase beta mediated NF-kappaB activation
Fong et al., J Exp Med 2008 (Inflammation...) : In response to proinflammatory cytokines and pathogen associated molecular patterns, NF-kappaB activation is controlled by IkappaB kinase (IKK)beta
Jang et al., Proc Natl Acad Sci U S A 2008 (Encephalitis) : Whereas luteolin had no effect on the LPS induced increase in NF-kappaB DNA binding activity, it markedly reduced AP-1 transcription factor binding activity
Tsai et al., Eur J Pharmacol 2008 : Our data suggest that paeonol inhibits osteoclastogenesis from bone marrow stromal cells and macrophage cells via attenuated of RANKL induced ERK, p38 and NF-kappaB activation, which in turn protect bone loss from ovariectomy
Yang et al., Nan Fang Yi Ke Da Xue Xue Bao 2008 (Colitis) : NF-kappa B activation is an important event in the development of DSS induced colitis in that activated NF-kappa B upregulates ICAM-1 expression during colonic inflammation
Kin et al., Shock 2008 : Compared with Control, Postcon also inhibited translocation of NF-kappa B to nuclei ( 167 % +/- 21 % vs. 142 % +/- 18 % * ), decreased the level of plasma TNF-alpha ( 1,994 +/- 447 vs. 667 +/- 130* pg/mL ), and inhibited caspase-3 activity ( 0.57 % +/- 0.1 % vs. 0.21 % +/- 0.1 % * )
Fukuda et al., Oncol Rep 2008 (Carcinoma, Adenoid Cystic...) : CYLD , a tumor suppressor gene, has deubiquitinating enzyme activity and inhibits the activation of transcription factor NF-kappaB ... The expression of CYLD and NF-kappaB mRNAs in HSG cells was increased by TNF-alpha
Görlach et al., Biochem J 2008 : One important mechanism underlying these responses is the transcriptional regulation of HIF-1alpha by the redox-sensitive transcription factor NF-kappaB ( nuclear factor kappaB ), which binds at a distinct element in the proximal promoter of the HIF-1alpha gene
Roumestan et al., Clin Exp Allergy 2008 : We then investigated whether these drugs affect NF-kappaB and AP-1 activities in A549 lung epithelial cells, and whether this potential regulation involves H1-receptor and 5-LO
Syed et al., Toxicol Appl Pharmacol 2008 (Breast Neoplasms...) : Delphinidin was found to repress HGF activated NFkappaB transcription with a decrease in ( i ) phosphorylation of IKKalpha/beta and IkappaBalpha, and ( ii ) activation and nuclear translocation of NFkappaB/p65
Tong et al., Cell Signal 2008 (Arthritis, Rheumatoid...) : The binding of p65 to the NF-kappaB elements, as well as the recruitment of p300 and the enhancement of histone H3 acetylation on the IL-8 promoter was enhanced by leptin , which was inhibited by wortmannin, Akt inhibitor or IRS-1 siRNA
Jiao et al., Cytokine 2008 (Respiratory Distress Syndrome, Adult...) : Furthermore, ApoA-I treatment could diminish LTA mediated NFkappaB nuclear translocation in macrophages
Prabhakaran et al., Brain Res Bull 2008 (Manganese Poisoning...) : Pretreatment with 7-NI, SN50 or vitamin E significantly attenuated increased iNOS expression indicating that iNOS expression is regulated by ROS and the transcription factor NF-kappaB ... These findings suggest that NF-kappaB induction and the activation of nitric oxide synthase through ROS represent a proximate mechanism for Mn-induced neurotoxicity
Giannopoulou et al., Am J Pathol 2008 (Inflammation...) : However, HGF prevented p65 NF-kappaB binding to its cognate cis acting element in the RANTES promoter
Hatao et al., FEMS Immunol Med Microbiol 2008 : Expression of either a deletion mutant of MyD88 lacking its death domain or MyD88s, neither of which induced NF-kappaB activation , did not lead to IRAK-4 downregulation
Le Negrate et al., Cell Microbiol 2008 (Chlamydia Infections) : ChlaDub1 of Chlamydia trachomatis suppresses NF-kappaB activation and inhibits IkappaBalpha ubiquitination and degradation
Bhaumik et al., Oncogene 2008 (Breast Neoplasms...) : Impaired NF-kappaB activity was evident from reduced phosphorylation of the NF-kappaB inhibitor IkappaBalpha, reduced NF-kappaB DNA binding activity and suppressed expression of the NF-kappaB target genes IL-8, IL-6 and matrix metalloproteinase-9 ... Impaired NF-kappaB activity was evident from reduced phosphorylation of the NF-kappaB inhibitor IkappaBalpha, reduced NF-kappaB DNA binding activity and suppressed expression of the NF-kappaB target genes IL-8 , IL-6 and matrix metalloproteinase-9
Mitola et al., Blood 2008 : Ang-1 up-regulation is mediated by the activation of the transcription factor NF-kappaB
Seki et al., Hepatology 2008 (Acute Disease...) : Toll-like receptors ( TLRs ) are pattern recognition receptors that recognize pathogen associated molecular patterns and signal through adaptor molecules, myeloid differentiation factor 88 ( MyD88 ), Toll/IL-1 receptor domain containing adaptor protein ( TIRAP ), Toll/IL-1 receptor domain containing adaptor inducing interferon-beta ( TRIF ), and TRIF related adaptor molecule ( TRAM ) to activate transcription factors, nuclear factor (NF)-kappaB , activator protein 1 (AP-1), and interferon regulatory factors ( IRFs ) leading to the initiation of innate immunity
Carbone et al., Brain Res 2008 (Inflammation...) : A role for NF-kappaB in MPTP dependent induction of NOS1 was confirmed through overexpression of a mutant IkappaBalpha super repressor of NF-kappaB that prevented induction of NOS1 ... The data presented here thus demonstrate a role for NF-kappaB in selective induction of NOS1 during early inflammatory activation of astrocytes stimulated by low-dose MPTP and inflammatory cytokines
Argueta-Donohué et al., Exp Parasitol 2008 : Leishmania mexicana : participation of NF-kappaB in the differential production of IL-12 in dendritic cells and monocytes induced by lipophosphoglycan ( LPG )
Gallel et al., Hum Pathol 2008 (Carcinoma, Medullary...) : The results also support the hypothesis that RET activation by somatic or germ line mutations may be responsible for NF-kappaB activation in MTCs
Zhou et al., Frontiers in bioscience : a journal and virtual library 2008 : Furthermore, we show that PsL-EGFmAb induced down-regulation of DC-SIGN may inhibit NF-kappaB expression in DCs, which accounts for the inhibition of DC maturation and stimulatory function
Wang et al., J Biol Chem 2008 : Together, these results suggest that MKP-1 is a NF-kappaB mediated prosurvival effector in attenuating JNK mediated pro-apoptotic response ; NF-kappaB/MKP-1 mediated negative JNK regulation represents a potential therapeutic target for adjusting cell radiosensitivity
Ueffing et al., Blood 2008 : In contrast to the general view that c-FLIP transcription is controlled predominantly by nuclear factor-kappaB (NF-kappaB) , induction of c-FLIP ( short ) in T cells was primarily mediated by the calcineurin-nuclear factor of activated T cells ( NFAT ) pathway
Koziczak-Holbro et al., Ernst Schering Foundation symposium proceedings 2007 : IRAK-4 KD cells were severely impaired in NF-kappaB , JNK, and p38 activation in response to IL-1beta or TLR7 ligand
Truhlar et al., J Mol Biol 2008 : IkappaBalpha binds to and inhibits the transcriptional activity of NF-kappaB via its ankyrin repeat ( AR ) domain ... These data demonstrate clearly that the coupled folding and binding of IkappaBalpha is critical for its precise control of NF-kappaB transcriptional activity
Merino et al., Nephrol Dial Transplant 2008 (Kidney Diseases) : This inflammatory response was mediated by intracellular signalling dependent on NF-kappaB , p38 MARK or c-Jun PI3K but not by MEK1/2 activation
Lee et al., Mol Cells 2008 : The decreased expression of iNOS and subsequent reduction of NO were due to inhibition of nuclear translocation of transcription factor NF-kappaB ... In addition, pretreatment with raloxifene reduced LPS induced Akt phosphorylation as well as NF-kappa B DNA binding activity and NF-kappa B-dependent reporter gene activity
Beck et al., EMBO J 2008 (Inflammation) : In this respect, we found that glucocorticoids counteract the recruitment of activated Mitogen- and Stress activated protein Kinase-1 ( MSK1 ) at inflammatory gene promoters resulting in the inhibition of NF-kappaB p65 transactivation and of concurrent histone H3 phosphorylation
Lee et al., Biochem Pharmacol 2008 (Arthritis, Experimental) : This compound also inhibited LPS induced NF-kappaB inducing kinase (NIK) and Akt phosphorylation, which are upstream of NF-kappaB activation
Wu et al., Toxicol Appl Pharmacol 2008 : Inhibition of NFkappaB activation did not block Zn2+ induced COX-2 expression
Buron et al., Invest Ophthalmol Vis Sci 2008 : Second, TFF1 interferes with caspase-9 and caspase-3 activation through an NF-kappaB induced increase in the expression of XIAP ( X-linked inhibitor of apoptosis protein )
Tacchini et al., J Biol Chem 2008 (Inflammation) : Role of HIF-1 and NF-kappaB transcription factors in the modulation of transferrin receptor by inflammatory and anti-inflammatory signals
Dan et al., Genes Dev 2008 (Prostatic Neoplasms) : Akt dependent regulation of NF-{kappa}B is controlled by mTOR and Raptor in association with IKK ... Akt dependent regulation of NF-{kappa}B is controlled by mTOR and Raptor in association with IKK ... Akt dependent regulation of NF-{kappa}B is controlled by mTOR and Raptor in association with IKK ... We show here that mTOR downstream from Akt controls NF-kappaB activity in PTEN-null/inactive prostate cancer cells via interaction with and stimulation of IKK ... The mTOR associated protein Raptor is required for the ability of Akt to induce NF-kappaB activity ... The mTOR associated protein Raptor is required for the ability of Akt to induce NF-kappaB activity
Lee et al., J Neurosci Res 2008 : BPA activated phosphorylation of extracellular signal regulated kinase ( ERK ) and c-Jun N-terminal kinase (JNK), and nuclear translocation of nuclear factor (NF)-kappaB
Luengo-Blanco et al., Blood 2008 (Ectodermal Dysplasia...) : This work investigated the functional role of nuclear factor-kappaB (NF-kappaB) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase genes CYBB, CYBA, NCF1, and NCF2 ... This work investigated the functional role of nuclear factor-kappaB (NF-kappaB) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase genes CYBB, CYBA , NCF1, and NCF2 ... This work investigated the functional role of nuclear factor-kappaB (NF-kappaB) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase genes CYBB , CYBA, NCF1, and NCF2 ... This work investigated the functional role of nuclear factor-kappaB (NF-kappaB) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase genes CYBB, CYBA, NCF1 , and NCF2 ... This work investigated the functional role of nuclear factor-kappaB (NF-kappaB) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase genes CYBB, CYBA, NCF1, and NCF2 ... These studies show that NF-kappaB is necessary for CYBB and NCF1 gene expression and activation of the phagocyte NADPH oxidase in this model system ... These studies show that NF-kappaB is necessary for CYBB and NCF1 gene expression and activation of the phagocyte NADPH oxidase in this model system ... These studies show that NF-kappaB is necessary for CYBB and NCF1 gene expression and activation of the phagocyte NADPH oxidase in this model system ... This work investigated the functional role of nuclear factor-kappaB (NF-kappaB) in respiratory burst activity and in expression of the human phagocyte nicotinamide adenine dinucleotide phosphate ( NADPH ) oxidase genes CYBB, CYBA, NCF1 , and NCF2
Henning et al., J Immunol 2008 : SP-A decreases the phosphorylation of IkappaBalpha , a key regulator of NF-kappaB activity, and nuclear translocation of p65 which result in diminished TNF-alpha secretion in response to TLR ligands ... Finally, we report for the first time that SP-A decreases the phosphorylation of Akt , a major cell regulator of NF-kappaB and potentially MAPKs
Chen et al., J Immunol 2008 : Proteasome inhibitors enhance TRAIL induced apoptosis through the intronic regulation of DR5 : involvement of NF-kappa B and reactive oxygen species mediated p53 activation ... Both p53 small interfering RNA and NF-kappaB inhibitor suppressed DR5 expression, strengthening the significance of p53 and NF-kappaB in DR5 transcription
Boesen et al., American journal of physiology. Renal physiology 2008 : IL-1beta activated NF-kappaB and increased ET-1 release in a concentration dependent manner
Li et al., Biochem Biophys Res Commun 2008 : Firstly, this NLS was found to be required for both RIP3 induced apoptosis and RIP3 mediated NF-kappaB activation
Tomizawa et al., Diab Vasc Dis Res 2008 : Adiponectin induces NF-kappaB activation that leads to suppression of cytokine induced NF-kappaB activation in vascular endothelial cells : globular adiponectin vs. high molecular weight adiponectin ... HMW adiponectin requires a shorter incubation period to demonstrate inhibition against tumour necrosis factor alpha (TNFalpha) induced NF-kappaB activation, compared with gAd
Holmes et al., Curr Med Chem 2008 (Leukemia...) : In contrast, GSK-3beta positively regulates NF-kappaB , another important biochemical pathway also involved in the regulation of multiple aspects of normal and aberrant haematopoiesis ... In addition, GSK-3beta regulates the pro-inflammatory activity of NF-kappaB
Bai et al., BMC infectious diseases 2008 : The effects of HIV-1 Tat on barrier function of the RPE may be mediated by ERK MAPK and NF-kappaB activation, which may represent potential targets for novel therapeutic approaches for the retinopathy induced by HIV infection
Fan et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2008 (Leukemia) : Ara-C can enhance CD86 and NF-kappaB expression on acute leukemia cells, which may play critical role in T cell activation and differentiation
Kim et al., Cell Biol Int 2008 (Bone Resorption) : Interestingly, licochalcone A was shown to inhibit the RANKL induced activation of extracellular signal regulated kinase, translocation of NF-kappaB into nucleus and mRNA expression of Fra-2
Yokozawa et al., J Agric Food Chem 2008 (Inflammation...) : Moreover, gravinol administration in the fructose fed rats markedly reduced the glycosylated protein and thiobarbituric acid-reactive substance levels in the serum and hepatic mitochondria, and it inhibited the increase of the cyclooxygenase-2 protein level as a result of the down-regulation of nuclear factor kappa B (NF-kappaB)
Poitevin et al., Journal of thrombosis and haemostasis : JTH 2008 : PDTC and NAC inhibited NF-kappaB activation during monocyte interaction with collagen I
Fischer et al., J Surg Res 2009 (Disease Models, Animal...) : Several potential mechanisms have been proposed to be involved in the myocardial dysfunction associated with mesenteric I/R, including nuclear factor kappa B (NF kappaB) mediated tumor necrosis factor alpha (TNF-alpha) release leading to cardiodepression
Sitcheran et al., Mol Cell Biol 2008 (Calcium Signaling) : The glutamate induced activation of NF-kappaB requires calcium dependent IkappaB kinase alpha (IKKalpha) and IKKbeta activation and induces p65-IkappaBalpha dissociation in the absence of IkappaBalpha phosphorylation or degradation ... The glutamate induced activation of NF-kappaB requires calcium dependent IkappaB kinase alpha (IKKalpha) and IKKbeta activation and induces p65-IkappaBalpha dissociation in the absence of IkappaBalpha phosphorylation or degradation ... The glutamate induced activation of NF-kappaB requires calcium dependent IkappaB kinase alpha (IKKalpha) and IKKbeta activation and induces p65-IkappaBalpha dissociation in the absence of IkappaBalpha phosphorylation or degradation
Ozsoy et al., J Biol Chem 2008 : The changes in TNFR self-association were functionally significant, because pretreating the HeLa cells and 293T cells resulted in increased TNF induced NF-kappaB activation and TNF induced expression of IkappaB and syndecan-4 mRNA levels ... Although pretreatment with DsbA did not result in an increase in TNF binding to TNFRs, it resulted in increased TNF induced activation of NF-kappaB , consistent with an allosteric modification of the TNFRs. Taken together, these results suggest that oxidative stress promotes TNFR receptor self-interaction and ligand independent and enhanced ligand dependent TNF signaling
Yang et al., J Neuroimmunol 2008 : The activation of NF-kappaB was followed by increased mRNA expression of TNF-alpha and IL-1beta peaking at about 20 h ... The activation of NF-kappaB was followed by increased mRNA expression of TNF-alpha and IL-1beta peaking at about 20 h
Lee et al., J Pharm Pharmacol 2008 : To further elucidate the mechanism of this inhibitory effect of eutigoside C, we studied LPS induced nuclear factor (NF)-kappaB activation and mitogen activated protein ( MAP ) kinase phosphorylation
Liu et al., Int Immunopharmacol 2008 : Inhibition of LPS induced NF-kappaB activation may contribute to the above immunosuppressive or anti-inflammatory activities of albaconol
Ganji et al., Atherosclerosis 2009 : Niacin inhibited : ( a ) angiotensin II (ANG II) induced reactive oxygen species ( ROS ) production by 24-86 %, ( b ) low density lipoprotein (LDL) oxidation by 60 %, ( c ) tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation by 46 %, vascular cell adhesion molecule-1 ( VCAM-1 ) by 77-93 %, monocyte chemotactic protein-1 (MCP-1) secretion by 34-124 %, and ( d ) in a functional assay TNF-alpha induced monocyte adhesion to HAEC ( 41-54 % )
Zhang et al., J Leukoc Biol 2008 (Burkitt Lymphoma) : Conversely, increasing tmTNF-alpha expression by suppressing expression of TNF-alpha converting enzyme that cleaves tmTNF-alpha led to an enhanced activation of NF-kappaB, indicating that tmTNF-alpha, but not sTNF-alpha , contributes to constitutive NF-kappaB activation
Cichocki et al., Mol Nutr Food Res 2008 : Recent studies showed that topically applied resveratrol significantly inhibited cyclooxygenase-2 (COX-2) expression and activation of nuclear factor-kappaB (NF-kappaB) induced by tumor promoter 12-O-tetradecanoylphorbol-13-acetate ( TPA ) in mouse epidermis ... Both stilbenes inhibited the activation of NF-kappaB by blocking the translocation of p65 to the nucleus and increasing the retention of IkappaBa in the cytosol
Eley et al., Cancer Chemother Pharmacol 2009 (Neoplasms, Experimental) : These results suggest that inhibitors of PKR may be useful therapeutic agents against tumours showing increased expression of PKR and constitutive activation of NF-kappaB , and may also prove useful in sensitising tumours to standard chemotherapeutic agents
Xu et al., Planta Med 2008 (Infarction, Middle Cerebral Artery...) : Pre-treatment with isosteviol reduced infarct volume, ameliorated cell death and infiltration of neutrocytes, improved neuro-locomotor activity, increased SOD activity, induced Bcl-2, suppressed lipid superoxidation and the expression of NF-kappaB , and therefore retarded necrosis and apoptosis of neurons and inflammation
Calame et al., Curr Opin Immunol 2008 : AP-1, NF-kappaB , and IRF4 also activate Blimp-1
Zhao et al., Gastroenterology 2008 (Liver Neoplasms...) : Furthermore, down-regulation of osteopontin inhibited drug induced NF-kappaB activation and sensitized HCC cells to chemotherapeutic agents in vitro, which led to complete regression of HCC xenografts in nude mice
Kanda et al., Endocrinology 2008 : IL-1beta enhanced the transcriptional activity of NF-kappaB , whereas leptin enhanced STAT1 and STAT3 activity ... IL-1beta enhanced the transcriptional activity of NF-kappaB , whereas leptin enhanced STAT1 and STAT3 activity ... IL-1beta enhanced the transcriptional activity of NF-kappaB , whereas leptin enhanced STAT1 and STAT3 activity ... IL-1beta enhanced the transcriptional activity of NF-kappaB , whereas leptin enhanced STAT1 and STAT3 activity ... The p38 MAPK inhibitor SB202190 suppressed IL-1beta- and IL-1beta plus leptin induced hBD-2 production, IL-1beta induced NF-kappaB activity, and leptin induced STAT1 and STAT3 activity ; contrastingly, the Janus kinase (JAK) 2 inhibitor AG490 suppressed IL-1beta plus leptin induced hBD-2 production and leptin induced STAT1 and STAT3 activity ... IL-1beta or leptin individually induced threonine/tyrosine phosphorylation of p38 MAPK, whereas only leptin induced tyrosine phosphorylation of JAK2, suggesting that leptin may enhance hBD-2 production in keratinocytes by activating STAT1 and STAT3 via JAK2 and p38 MAPK in cooperation with NF-kappaB , which is activated by IL-1beta
Hosokawa et al., J Periodontal Res 2008 (Periodontal Diseases) : Furthermore, a c-Jun NH2 terminal kinase (JNK) inhibitor, a phosphatidylinositol-3-kinase (PI3K) inhibitor and a nuclear factor kappa B (NF-kappa B) inhibitor inhibited CCL17 production by HGFs
Zare et al., J Leukoc Biol 2008 : In contrast, Plg production did not require NF-kappaB , was only partly down-regulated by p38 MAPK inhibitor, and was efficiently inhibited by insulin , indicating a different mechanism for its induction ... In contrast, Plg production did not require NF-kappaB , was only partly down-regulated by p38 MAPK inhibitor, and was efficiently inhibited by insulin, indicating a different mechanism for its induction ... In contrast, Plg production did not require NF-kappaB , was only partly down-regulated by p38 MAPK inhibitor, and was efficiently inhibited by insulin, indicating a different mechanism for its induction ... In contrast, Plg production did not require NF-kappaB , was only partly down-regulated by p38 MAPK inhibitor, and was efficiently inhibited by insulin, indicating a different mechanism for its induction
Wilson et al., J Virol 2008 : We report that expression of WNV NS1 inhibits TLR3 induced transcriptional activation of the IFN-beta promoter and of an NF-kappaB-responsive promoter ... We report that expression of WNV NS1 inhibits TLR3 induced transcriptional activation of the IFN-beta promoter and of an NF-kappaB-responsive promoter
Babu et al., Arch Pharm Res 2008 : Taken together, our results suggest that the anti-oxidant components of EPS prevent TNF-alpha induced NF-kappaB activation, chemokine induction, and monocyte adhesion at the site of intestinal inflammation
Zhou et al., Exp Neurol 2008 : Nuclear factor-kappaB (NF-kappaB) inhibitor ( PDTC ) also suppressed spinal BDNF-LTP
Solanas et al., J Cell Sci 2008 : E-cadherin controls beta-catenin and NF-kappaB transcriptional activity in mesenchymal gene expression ... E-cadherin overexpression decreased the transcriptional activity of the fibronectin promoter and reduced the interaction of beta-catenin and NF-kappaB with this promoter
Yan et al., Mol Cancer Ther 2008 (Neoplasms) : 17-AJB effectively inhibited tumor necrosis factor-alpha induced NF-kappaB activation and induced apoptosis of tumor cells
Sethi et al., Mol Cancer Ther 2008 (Neoplasm Invasiveness...) : Pinitol also suppressed the NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)-1 , TNFR associated death domain, TNFR associated factor-2, transforming growth factor-beta activated kinase-1 ( TAK-1 ) /TAK1 binding protein-1, and IkappaBalpha kinase but not that induced by p65 ... Pinitol also suppressed the NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)-1 , TNFR associated death domain, TNFR associated factor-2, transforming growth factor-beta activated kinase-1 ( TAK-1 ) /TAK1 binding protein-1, and IkappaBalpha kinase but not that induced by p65 ... Pinitol also suppressed the NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)-1, TNFR associated death domain, TNFR associated factor-2, transforming growth factor-beta activated kinase-1 ( TAK-1 ) /TAK1 binding protein-1 , and IkappaBalpha kinase but not that induced by p65 ... Pinitol also suppressed the NF-kappaB reporter activity induced by tumor necrosis factor receptor (TNFR)-1, TNFR associated death domain, TNFR associated factor-2, transforming growth factor-beta activated kinase-1 ( TAK-1 ) /TAK1 binding protein-1, and IkappaBalpha kinase but not that induced by p65
Hailemariam et al., Arterioscler Thromb Vasc Biol 2008 : Sphingomyelin synthase 2 deficiency attenuates NFkappaB activation
Cheng et al., J Immunol 2008 : HeLa cells expressing TLR2 responded to SAA with potent activation of NF-kappaB , which was enhanced by TLR1 expression and blocked by the Toll/IL-1 receptor/resistance ( TIR ) deletion mutants of TLR1, TLR2, and TLR6
Kaneko et al., J Immunol 2008 : In addition, activation of NF-kappaB and lipid peroxidation were induced by cis-UCA and SSR , but not trans-UCA, suggesting possible upstream events of the gene expression changes ... In addition, activation of NF-kappaB and lipid peroxidation were induced by cis-UCA and SSR, but not trans-UCA, suggesting possible upstream events of the gene expression changes
Bonfield et al., J Immunol 2008 : Taken together, these data suggest that M-CSF is an important mediator of alveolar macrophage homeostasis, and that transcriptional control of M-CSF production is regulated by NF-kappaB and PPARgamma
Zand et al., Mol Cell Biochem 2008 : Pretreatment of Ramos cells with GW9662 abrogated the ability of DHA to inhibit Gamma-IR induced activation of NF-kappaB and diminished the DHA radiosensitizing effect indicating that PPAR-gamma may act as a mediator of DHA in inhibition of NF-kappaB ... Pretreatment of Ramos cells with GW9662 abrogated the ability of DHA to inhibit Gamma-IR induced activation of NF-kappaB and diminished the DHA radiosensitizing effect indicating that PPAR-gamma may act as a mediator of DHA in inhibition of NF-kappaB
Sethi et al., Mol Cancer Res 2008 : As examined by DNA binding, we found that TQ suppressed tumor necrosis factor induced NF-kappa B activation in a dose- and time dependent manner and inhibited NF-kappaB activation induced by various carcinogens and inflammatory stimuli
Kota et al., J Biol Chem 2008 : Our results demonstrated that HBD2 is induced by TNF and RSV in an NF-kappaB dependent manner
Deree et al., Clinics (Sao Paulo) 2008 (Sepsis) : The increased level of cAMP that results from phosphodiesterase inhibition affects cytoplasmic and nuclear events, resulting in the attenuation of NF-kB and the activation of CREB transcriptional DNA binding through pathways that are partially Protein kinase A-independent
Huang et al., Immunol Invest 2008 : This effect was correlated with reduced LPS induced TNF-alpha mRNA and NF-kappaB activity in THP-1 cells
Zhao et al., Immunol Invest 2008 (Inflammation) : The cellular secretion of TNF-alpha, IL-1beta, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-alpha , COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, and activation of NF-kappaB were assayed by ELISA, the Griess method, real-time quantitative PCR, and Western blot and immunocytochemistry method, respectively ... The cellular secretion of TNF-alpha, IL-1beta, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-alpha, COX-2, iNOS and HO-1 , protein expression of COX-2 and HO-1, and activation of NF-kappaB were assayed by ELISA, the Griess method, real-time quantitative PCR, and Western blot and immunocytochemistry method, respectively ... The cellular secretion of TNF-alpha, IL-1beta, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-alpha, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, and activation of NF-kappaB were assayed by ELISA, the Griess method, real-time quantitative PCR, and Western blot and immunocytochemistry method, respectively ... The cellular secretion of TNF-alpha, IL-1beta, IL-6, NO and IL-10 in supernatant, mRNA expression of TNF-alpha, COX-2, iNOS and HO-1, protein expression of COX-2 and HO-1, and activation of NF-kappaB were assayed by ELISA, the Griess method, real-time quantitative PCR, and Western blot and immunocytochemistry method, respectively
Sadik et al., Int J Biochem Cell Biol 2008 (Lyme Disease) : Systematic analysis highlights the key role of TLR2/NF-kappaB/MAP kinase signaling for IL-8 induction by macrophage-like THP-1 cells under influence of Borrelia burgdorferi lysates
Xu et al., Mol Immunol 2008 : PTP1B inhibits TLR ligands induced activation of MAPKs, NF-kappaB , and IRF3, furthermore, co-transfection of PTP1B inhibits both MyD88- and TRIF induced transcription of TNF-alpha and IFN-beta reporter genes in a dose dependent manner ... PTP1B inhibits TLR ligands induced activation of MAPKs, NF-kappaB , and IRF3, furthermore, co-transfection of PTP1B inhibits both MyD88- and TRIF induced transcription of TNF-alpha and IFN-beta reporter genes in a dose dependent manner
Railo et al., Exp Cell Res 2008 : We demonstrate here that Wnt-11 signaling is sufficient to inhibit not only the canonical beta-catenin mediated Wnt signaling but also JNK/AP-1 and NF-kappaB signaling in the CHO cells, thus serving as a noncanonical Wnt ligand in this system
Kim et al., Biochem Pharmacol 2008 : LYR-71 inhibited IKKbeta mediated phosphorylation of cytoplasmic IkappaBalpha in lipopolysaccharide (LPS) activated macrophages, and sequentially preventing IkappaBalpha degradation as well as transcriptional activation of NF-kappaB ... LYR-71 inhibited IKKbeta mediated phosphorylation of cytoplasmic IkappaBalpha in lipopolysaccharide (LPS) activated macrophages, and sequentially preventing IkappaBalpha degradation as well as transcriptional activation of NF-kappaB ... Taken together, LYR-71 was an efficient inhibitor of IKKbeta , preventing NF-kappaB activation in macrophages, and this mechanism of action could contribute its down-regulatory effect on LPS induced expression of inflammatory cytokines at the transcription level
Ikeda et al., Mol Cell Biol 2008 : STAP-2 negatively regulates both canonical and noncanonical NF-kappaB activation induced by Epstein-Barr virus derived latent membrane protein 1 ... In the present study, we found that ectopic expression of STAP-2 inhibited Epstein-Barr virus ( EBV ) LMP1 mediated NF-kappaB signaling and interleukin-6 expression ... In the present study, we found that ectopic expression of STAP-2 inhibited Epstein-Barr virus ( EBV ) LMP1 mediated NF-kappaB signaling and interleukin-6 expression ... We found, furthermore, that STAP-2 regulated LMP1 mediated NF-kappaB signaling through direct or indirect interactions with the tumor necrosis factor receptor (TNFR) associated factor 3 ( TRAF3 ) and TNFR associated death domain (TRADD) proteins ... We found, furthermore, that STAP-2 regulated LMP1 mediated NF-kappaB signaling through direct or indirect interactions with the tumor necrosis factor receptor (TNFR) associated factor 3 ( TRAF3 ) and TNFR associated death domain (TRADD) proteins
Tang et al., J Exp Med 2008 (Bone Marrow Diseases...) : Activation of TAK1 by proinflammatory cytokines and T and B cell receptors induces the nuclear localization of nuclear factor kappaB (NF-kappaB) and the activation of c-Jun N-terminal kinase (JNK)/AP1 and P38, which play important roles in mediating inflammation, immune responses, T and B cell activation, and epithelial cell survival ... Activation of TAK1 by proinflammatory cytokines and T and B cell receptors induces the nuclear localization of nuclear factor kappaB (NF-kappaB) and the activation of c-Jun N-terminal kinase (JNK)/AP1 and P38 , which play important roles in mediating inflammation, immune responses, T and B cell activation, and epithelial cell survival ... Activation of TAK1 by proinflammatory cytokines and T and B cell receptors induces the nuclear localization of nuclear factor kappaB (NF-kappaB) and the activation of c-Jun N-terminal kinase (JNK)/AP1 and P38, which play important roles in mediating inflammation, immune responses, T and B cell activation, and epithelial cell survival ... Activation of TAK1 by proinflammatory cytokines and T and B cell receptors induces the nuclear localization of nuclear factor kappaB (NF-kappaB) and the activation of c-Jun N-terminal kinase (JNK)/AP1 and P38, which play important roles in mediating inflammation, immune responses, T and B cell activation, and epithelial cell survival
Guo et al., Antioxid Redox Signal 2008 (MAP Kinase Signaling System...) : Incubation of AOPPs, either prepared in vitro or isolated from uremic serum, with human umbilical vein ECs induced superoxide generation, activation of NAD ( P ) H oxidase , ERK 1/2 and p38, and nuclear translocation of NF-kappaB
Vandenberghe et al., Biochem Pharmacol 2008 : Indeed, physalin B induced an increase in bioluminescence from DLD-1 4Ub-Luc cells, at concentrations also producing an accumulation of ubiquitinated proteins and inhibiting TNFalpha induced NF-kappaB activation
Kasinski et al., Mol Pharmacol 2008 : The effective inhibition of TNF-alpha induced NF-kappaB signaling by EF24 extends the therapeutic application of EF24 to other NF-kappaB dependent diseases, including inflammatory diseases such as rheumatoid arthritis
Chae et al., Blood 2008 (Familial Mediterranean Fever) : The familial Mediterranean fever protein, pyrin, is cleaved by caspase-1 and activates NF-kappaB through its N-terminal fragment
Jung et al., J Biol Chem 2008 : We recently showed that a novel disulfide reductase, TRP14, inhibits tumor necrosis factor alpha (TNFalpha) induced NF-kappaB activation, and we identified the dynein light chain LC8, which interacts with the NF-kappaB inhibitor IkappaBalpha, as a potential substrate of TRP14 ... LC8 inhibited TNFalpha induced NF-kappaB activation in HeLa cells by interacting with IkappaBalpha and thereby preventing its phosphorylation by IkappaB kinase (IKK), without affecting the activity of IKK itself
O'Brien et al., J Biol Chem 2008 (Helicobacter Infections) : H. pylori induced expression of DAF was dependent upon activation of the p38 mitogen activated protein kinase pathway but not NF-kappaB
Calvisi et al., Carcinogenesis 2008 (Carcinoma, Hepatocellular...) : Suppression of iNOS signaling by aminoguanidine led to decreased HCC growth and NF-kB and RAS/ERK expression and increased apoptosis both in vivo and in vitro
Vender et al., Neurosurgery 2008 (Pituitary Neoplasms) : Pharmacological or molecular inhibition of Akt similarly reduced GH3 viability and NFkappaB binding, suggesting that constitutive activation of NFkappaB may be, at least in part, mediated by Akt
Dasgupta et al., Hybridoma (Larchmt) 2008 : On the other hand, antibodies produced from clones a3-3a and a3-7g specifically recognized p40 and inhibited p40- but not p40 ( 2 ) -, IL-12-, and IL-23 induced production of inflammatory molecules and activation of NF-kappaB
Qiu et al., Dev Comp Immunol 2008 : The over-expression of chicken MyD88-2 significantly induced the activation of NF-kappaB in vitro, suggesting that chicken MyD88-2 plays an important role in the innate immune responses of chicken
Madonna et al., Biochim Biophys Acta 2008 : Insulin markedly increased TNF-alpha induced NF-kappaB activation and induced phosphorylated IkappaB-alpha accumulation ... Insulin markedly increased TNF-alpha induced NF-kappaB activation and induced phosphorylated IkappaB-alpha accumulation ... Insulin markedly increased TNF-alpha induced NF-kappaB activation and induced phosphorylated IkappaB-alpha accumulation
Ahmad et al., Blood 2008 : Altogether, these results unequivocally show that HSV-1 induces TLR2 dependent activation of IL-15 gene expression, which requires the recruitment of both MyD88 and TIRAP/Mal and the activation of IRAK1 and TRAF6 leading to NF-kappaB translocation to the nucleus ... Altogether, these results unequivocally show that HSV-1 induces TLR2 dependent activation of IL-15 gene expression, which requires the recruitment of both MyD88 and TIRAP/Mal and the activation of IRAK1 and TRAF6 leading to NF-kappaB translocation to the nucleus ... Altogether, these results unequivocally show that HSV-1 induces TLR2 dependent activation of IL-15 gene expression, which requires the recruitment of both MyD88 and TIRAP/Mal and the activation of IRAK1 and TRAF6 leading to NF-kappaB translocation to the nucleus ... Altogether, these results unequivocally show that HSV-1 induces TLR2 dependent activation of IL-15 gene expression, which requires the recruitment of both MyD88 and TIRAP/Mal and the activation of IRAK1 and TRAF6 leading to NF-kappaB translocation to the nucleus
Wu et al., EMBO J 2008 : Activation of NF-kappaB in response to DNA double strand break ( DSB ) inducers can be mediated by ATM ( ataxia telangiectasia mutated ) -dependent phosphorylation of NEMO ( NF-kappaB essential modulator ) ... Consequently, ATR represses NF-kappaB activation induced by replication stress ... Reduction or increase of ATR expression by RNA interference or overexpression increased or reduced ATM-NEMO association and NF-kappaB activation induced by HU
Shaw et al., Curr Opin Immunol 2008 : Stimulation of NOD1 and NOD2, two prototypic NLRs, results in the activation of MAPK and NF-kappaB ... Stimulation of NOD1 and NOD2 , two prototypic NLRs, results in the activation of MAPK and NF-kappaB
de Seigneux et al., J Biol Chem 2008 : Chromatin immunoprecipitation showed that LPS increased p65 binding to two NF-kappaB sites along the SGK1 promoter
Zhang et al., J Mol Endocrinol 2008 (Inflammation) : PEDF also attenuated the HOG-LDL induced NF-kappaB activation, suggesting that the inhibitory effect of PEDF on MCP-1 was at least partially through the blockade of NF-kappaB activation ... PEDF also attenuated the HOG-LDL induced NF-kappaB activation, suggesting that the inhibitory effect of PEDF on MCP-1 was at least partially through the blockade of NF-kappaB activation
Huey et al., Exp Physiol 2008 (Acute Disease...) : The major results provide the first in vivo evidence that short-term vitamin E administration reduces IL-6 and IL-1beta responses to LPS in skeletal and cardiac muscle and prevents LPS induced increases in NF-kappaB activation and total oxidized proteins
Callister et al., Br J Pharmacol 2008 : PMX464 inhibited IL-1beta induced NF-kappaB DNA binding, nuclear translocation of NF-kappaB p65 subunit and factors involved in NF-kappaB activation ; specifically, IkappaBalpha degradation, IkappaB phosphorylation and IkappaB kinase (IKK) activity in A549
Singleton et al., Biochem Biophys Res Commun 2008 (Sepsis) : Glutamine attenuates inflammation and NF-kappaB activation via Cullin-1 deneddylation
Chen et al., Zhongguo Zhong Yao Za Zhi 2008 : To study the effect of total glucosides of paeony (TGP) on lipopolysaccharides (LPS) induced nuclear factor-kappaB (NF-kappaB) activation in macrophages ... To study the effect of total glucosides of paeony (TGP) on lipopolysaccharides (LPS) induced nuclear factor-kappaB (NF-kappaB) activation in macrophages ... TGP also inhibited the LPS mediated DNA binding activity of NF-kappaB complex in macrophages ... TGP can inhibit LPS induced NF-kappaB activation in macrophages through arresting IKBalpha protein degradation, NF-kappaB p65 protein nuclear translocation and DNA binding activity of NF-kappaB complex ... TGP can inhibit LPS induced NF-kappaB activation in macrophages through arresting IKBalpha protein degradation, NF-kappaB p65 protein nuclear translocation and DNA binding activity of NF-kappaB complex
Jin et al., Biol Pharm Bull 2008 : To clarify the mechanistic basis for DPT 's ability to inhibit iNOS induction, we examined the effect of DPT on nuclear factor (NF)-kappaB transcriptional activity and DNA binding activity
Gozzelino et al., Cell Res 2008 : Furthermore, Bcl-x ( L ) downregulation does not affect TNF-alpha mediated NF-kappaB activation
Kim et al., J Endocrinol 2008 (Insulinoma) : Overexpression of MEKK3 , a possible mediator from the TNF-alpha receptor to the JNK/p38 and NK-kappaB signaling cascade, increased the levels of phospho-JNK, phospho-p38, and NF-kappaB , and reduced the glucose stimulated Ca(2) ( + ) influx and GSIS
Ma et al., Cancer Chemother Pharmacol 2009 (Breast Neoplasms) : The objective of this study is to investigate the in vitro effects of epoxypseudoisoeugenol-2-methyl butyrate ( EPB ), a phenylpropranoid isolated from Pimpinella corymbosa, on the activation of NF-kappaB , cell growth, cell cycle progression and apoptosis in MCF-7 ( estrogen dependent ) and BT-549 ( estrogen independent ) breast cancer cells ... EPB also inhibited constitutive NF-kappaB transcriptional activity in BT-549 cells
Hamer et al., Biol Cell 2009 (Neoplasm Invasiveness...) : The up-regulation of cathepsin B by NF-kappaB , followed by its secretion into the extracellular environment, might be partly responsible for the previously reported invasiveness of the mtDNA depleted 143B osteosarcoma cells
Bianchi et al., Neurobiol Aging 2010 (Encephalitis...) : We have reported that RAGE ligation by S100B in BV-2 microglia results in the upregulation of expression of the pro-inflammatory cyclo-oxygenase 2 (COX-2) via parallel Ras-Cdc42-Rac1 dependent activation of c-Jun NH ( 2 ) terminal protein kinase ( JNK ) and Ras-Rac1 dependent stimulation of NF-kappaB transcriptional activity ... We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2, IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1 ... We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2, IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1 ... We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2, IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1 ... We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2, IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1 ... We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2 , IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1 ... We show here that : ( 1 ) S100B also stimulates AP-1 transcriptional activity in microglia via RAGE dependent activation of JNK ; ( 2 ) S100B upregulates IL-1beta and TNF-alpha expression in microglia via RAGE engagement ; and ( 3 ) S100B/RAGE induced upregulation of COX-2, IL-1beta and TNF-alpha expression requires the concurrent activation of NF-kappaB and AP-1
Lin et al., J Ethnopharmacol 2008 (Shock, Septic) : These results suggested that ASE protected mice against LPS/D-GalN induced endotoxic shock involving inhibition of NF-kappaB activation, which caused down-regulation of TNF-alpha and involved up-regulation of IL-10
Csiszar et al., J Appl Physiol 2008 (Cardiovascular Diseases...) : In the present review we summarize recent experimental data suggesting that mitochondrial production of reactive oxygen species, innate immunity, the local TNF-alpha converting enzyme (TACE)-TNF-alpha , and the renin-angiotensin system may underlie NF-kappaB induction and endothelial activation in aged arteries ... In the present review we summarize recent experimental data suggesting that mitochondrial production of reactive oxygen species, innate immunity, the local TNF-alpha converting enzyme (TACE)-TNF-alpha , and the renin-angiotensin system may underlie NF-kappaB induction and endothelial activation in aged arteries ... We also discuss the possibility that nucleophosmin ( NPM or nuclear phosphoprotein B23 ), a known modulator of the cellular oxidative stress response, may also regulate NF-kappaB activity in endothelial cells
Kim et al., J Microbiol Biotechnol 2008 (MAP Kinase Signaling System) : These results suggest that the synergistic effect of PGA1 on LPS induced IL-10 expression is NF-kappaB dependent and mediated by mitogen activated protein ( MAP ) kinases, p38, and SAPK/ JNK signaling pathways, and also associated with the PPARgamma pathway
Sarkar et al., Int Immunopharmacol 2008 (Edema) : APC inhibited LPS induced nuclear factor-kappaB (NF-kappaB) activation, by preventing degradation of the inhibitor kappaB ( IkappaB )
Kim et al., Cancer Lett 2008 : As examined by the DNA binding of NF-kappaB activation, we found that PTX-2 suppressed constitutive NF-kappaB activation and determined by p65 and p50 nuclear translocation, and IkappaBalpha degradation through dephosphorylation of Akt ... In addition, treatment of PTX-2 down-regulated NF-kappaB dependent gene expression, Cox-2, IAP-1, IAP-2 and XIAP, at the transcriptional and translational level ... In addition, treatment of PTX-2 down-regulated NF-kappaB dependent gene expression, Cox-2, IAP-1 , IAP-2 and XIAP, at the transcriptional and translational level ... In addition, treatment of PTX-2 down-regulated NF-kappaB dependent gene expression, Cox-2, IAP-1, IAP-2 and XIAP , at the transcriptional and translational level ... In addition, treatment of PTX-2 down-regulated NF-kappaB dependent gene expression, Cox-2 , IAP-1, IAP-2 and XIAP, at the transcriptional and translational level
Lai et al., J Ethnopharmacol 2008 (Gastritis) : Furthermore, H. pylori induced nuclear factor (NF)-kappaB activation, and the subsequent release of interleukin (IL)-8 in AGS cells were also inhibited by the extract PUC
Bai et al., Neurosci Res 2008 (Nerve Degeneration...) : p75 ( NTR ) activation of NF-kappaB is involved in PrP106-126 induced apoptosis in mouse neuroblastoma cells ... For the first time we found that PrP106-126 induced activation of NF-kappaB by Western blot assay, and blocking the interaction of p75 ( NTR ) with PrP106-126 by p75 ( NTR ) polyclonal antibody sc-6189 or pretreatment with inhibitor NF-kappaB SN50 reduced the activation of NF-kappaB and attenuated the apoptotic effect by PrP106-126
Lee et al., Food Chem Toxicol 2008 (Necrosis) : Hydrogen peroxide provoked phosphorylation of extracellular regulated kinase (ERK) and c-Jun NH ( 2 ) -terminal kinase ( JNK ), and activation of nuclear factor-kappaB (NF-kappaB) ... Hydrogen peroxide provoked phosphorylation of extracellular regulated kinase (ERK) and c-Jun NH ( 2 ) -terminal kinase ( JNK ), and activation of nuclear factor-kappaB (NF-kappaB)
Funakoshi-Tago et al., Cell Signal 2008 : Consequently, p38, JNK and Nuclear factor-kappaB (NF-kappaB) activation induced by IL-33 was completely inhibited in TRAF6-deficient MEFs ... Moreover, IL-33 augmented autoubiquitination of TRAF6 and the reconstitution of TRAF6 mutant ( C70A ) that is defective in its ubiquitin ligase activity failed to restore IL-33 induced p38, JNK and NF-kappaB activation
Kamiyama et al., Biosci Biotechnol Biochem 2008 : cDNA microarray analysis revealed that EPQB decreased TNF-alpha induced expression of NF-kappaB target genes
Pokharel et al., Evidence-based complementary and alternative medicine : eCAM 2008 : In this study, SYT selectively suppressed NF-kappaB activation, which may be essential for TARC expression in TNF-alpha/IFN-gamma treated keratinocytes ... The inhibitory effect of SYT on NF-kappaB activation and TARC production might be associated with the anti-dermatitic effects of SYT
Aravalli et al., Journal of neuroinflammation 2008 (Histoplasmosis) : Using a 293T stable cell line expressing murine Toll-like receptor 2 [TLR2 ], we show here that TLR2 recognizes H. capsulatum cell wall protein Yps3p and induces the activation of NF-kappaB
Zhang et al., Chin J Cancer 2008 (Lung Neoplasms...) : Transfection of IkappaBalpha can inhibit NF-kappaB activity, thus inhibit cell invasion of A549, which may be through the down-regulation of MMP-2 and MMP-9 expressions
Sethi et al., Biochem Pharmacol 2008 (Neoplasm Invasiveness) : SH-5 also blocked NF-kappaB activation induced by TNF-alpha , lipopolysaccharide, phorbol ester, and cigarette smoke but not that activated by hydrogen peroxide and RANK ligand, indicating differential requirement of AKT ... This led to suppression of the phosphorylation and translocation of p65 and also of NF-kappaB reporter activity induced by TNFR1 , TRADD, TRAF2, NIK, and IKKbeta but not that induced by p65 transfection ... This led to suppression of the phosphorylation and translocation of p65 and also of NF-kappaB reporter activity induced by TNFR1, TRADD , TRAF2, NIK, and IKKbeta but not that induced by p65 transfection ... This led to suppression of the phosphorylation and translocation of p65 and also of NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2 , NIK, and IKKbeta but not that induced by p65 transfection ... This led to suppression of the phosphorylation and translocation of p65 and also of NF-kappaB reporter activity induced by TNFR1, TRADD, TRAF2, NIK, and IKKbeta but not that induced by p65 transfection
Spirig et al., J Immunol 2008 : Moreover, DXS was found to inhibit phosphorylation of IkappaB-alpha and activation of NF-kappaB
Hara et al., J Immunol 2008 : Cell type-specific regulation of ITAM mediated NF-kappaB activation by the adaptors, CARMA1 and CARD9 ... Cell type-specific regulation of ITAM mediated NF-kappaB activation by the adaptors, CARMA1 and CARD9 ... Although the caspase recruitment domain (CARD)9-Bcl10 complex is essential for FcRgamma/DAP12 mediated NF-kappaB activation in myeloid cells, its involvement in NK cell receptor signaling is unknown ... Although the caspase recruitment domain (CARD)9-Bcl10 complex is essential for FcRgamma/DAP12 mediated NF-kappaB activation in myeloid cells, its involvement in NK cell receptor signaling is unknown ... Although the caspase recruitment domain (CARD)9-Bcl10 complex is essential for FcRgamma/DAP12 mediated NF-kappaB activation in myeloid cells, its involvement in NK cell receptor signaling is unknown ... Although the caspase recruitment domain (CARD)9-Bcl10 complex is essential for FcRgamma/DAP12 mediated NF-kappaB activation in myeloid cells, its involvement in NK cell receptor signaling is unknown ... Surprisingly, CARMA1 deficiency also abrogated P/I induced IKK activation in DCs, indicating that CARMA1 is essential for PKC mediated NF-kappaB activation in all cell types, although the PKC-CARMA1 axis is not used downstream of myeloid ITAM receptors
Travert et al., J Immunol 2008 (Lymphoma, Follicular) : Thus, the antiapoptotic signaling of CD40, which interferes with TRAIL induced apoptosis in FL B cells, involves NF-kappaB mediated induction of c-FLIP and Bcl-x ( L ) which can respectively interfere with caspase 8 activation or mitochondrial mediated apoptosis ... Thus, the antiapoptotic signaling of CD40, which interferes with TRAIL induced apoptosis in FL B cells, involves NF-kappaB mediated induction of c-FLIP and Bcl-x ( L ) which can respectively interfere with caspase 8 activation or mitochondrial mediated apoptosis
Pincheira et al., J Immunol 2008 : TNFR1/c-Src, but not TNFR1/Jak2, plays an obligate role in the activation of NF-kappaB by TNF , whereas TNFR1/Jak2, but not TNFR1/c-Src, plays an obligate role in the activation of STAT3 ... TNFR1/c-Src , but not TNFR1/Jak2, plays an obligate role in the activation of NF-kappaB by TNF, whereas TNFR1/Jak2, but not TNFR1/c-Src, plays an obligate role in the activation of STAT3 ... TNFR1/c-Src, but not TNFR1/Jak2 , plays an obligate role in the activation of NF-kappaB by TNF, whereas TNFR1/Jak2, but not TNFR1/c-Src, plays an obligate role in the activation of STAT3 ... TNFR1/c-Src, but not TNFR1/Jak2 , plays an obligate role in the activation of NF-kappaB by TNF, whereas TNFR1/Jak2, but not TNFR1/c-Src, plays an obligate role in the activation of STAT3
Ju et al., J Immunol 2008 (Arthritis, Experimental...) : IL-23 induces receptor activator of NF-kappaB ligand expression on CD4+ T cells and promotes osteoclastogenesis in an autoimmune arthritis model
Rasmussen et al., Inflamm Res 2008 (Inflammation) : We studied NF-kappaB activation and the induction of interleukin 8 (IL-8) and p53 gene expression in an interleukin 1beta (IL-1beta) stimulated HepG2 cell line ... We studied NF-kappaB activation and the induction of interleukin 8 (IL-8) and p53 gene expression in an interleukin 1beta (IL-1beta) stimulated HepG2 cell line ... NF-kappaB induced IL-8 and p53 protein production was studied using specific siRNA, an IkappaB kinase 2 inhibitor, and mitogen activated protein kinase ( MAPK ) inhibitors ... NF-kappaB induced IL-8 and p53 protein production was studied using specific siRNA, an IkappaB kinase 2 inhibitor, and mitogen activated protein kinase ( MAPK ) inhibitors
Lee et al., Dig Dis Sci 2009 (Colitis) : Saccharomyces boulardii blocked tumor necrosis factor-alpha (TNF-alpha) regulation of PPAR-gamma and IL-8 through disruption of TNF-alpha mediated nuclear factor kappa B (NF-kappaB) activation
Rajan et al., J Cell Biochem 2008 : We further demonstrated that inhibition of NF-kappaB completely blocked TNF-alpha induced expression of ICAM-1, VCAM-1 and E-selectin ... We further demonstrated that inhibition of NF-kappaB completely blocked TNF-alpha induced expression of ICAM-1, VCAM-1 and E-selectin ... We further demonstrated that inhibition of NF-kappaB completely blocked TNF-alpha induced expression of ICAM-1 , VCAM-1 and E-selectin ... Taken together, our results clearly demonstrate that NF-kappaB , but not p38, is critical for TNF-alpha induced CAM expression
Alff et al., J Virol 2008 : The formation of TBK1-TRAF3 complexes directs IRF-3 phosphorylation, and both IRF-3 and NF-kappaB activation are required for transcription from the IFN-beta promoter ... The formation of TBK1-TRAF3 complexes directs IRF-3 phosphorylation, and both IRF-3 and NF-kappaB activation are required for transcription from the IFN-beta promoter ... In contrast, the Gn tail of the nonpathogenic hantavirus Prospect Hill virus ( PHV ) failed to coprecipitate TRAF3 or inhibit NF-kappaB or IFN-beta transcriptional responses
Ulivi et al., J Cell Physiol 2008 (Inflammation) : Experimental evidences being the following : ( 1 ) 15-deoxy-delta 12,14-prostaglandin J ( 2 ), but not PGE ( 2 ) : ( i ) inhibits the expression of SIP24 in the inflammatory phase and induces COX-2 synthesis ; ( ii ) represses NF-kB activation induced by LPS ; ( iii ) represses the synthesis of microsomal PGE Synthase-1 induced by LPS
van Keulen et al., Atherosclerosis 2009 (Carotid Artery Diseases...) : Tlr activation results in nuclear translocation of the transcription factor Nuclear Factor-kappa B (NF-kappaB) that controls the transcription of many inflammatory genes
Yu et al., J Biol Chem 2008 : Phosphorylation of Thr-178 and Thr-184 in the TAK1 T-loop is required for interleukin (IL)-1 mediated optimal NFkappaB and AP-1 activation as well as IL-6 gene expression ... Consistently, TAK1 mutant with alanine substitution of these two residues severely inhibits IL-1 induced NFkappaB and AP-1 activities, whereas TAK1 mutant with replacement of these two sites with acidic residues slightly enhances IL-1 induced NFkappaB and AP-1 activities compared with the TAK1 wild-type ... Consistently, TAK1 mutant with alanine substitution of these two residues severely inhibits IL-1 induced NFkappaB and AP-1 activities, whereas TAK1 mutant with replacement of these two sites with acidic residues slightly enhances IL-1 induced NFkappaB and AP-1 activities compared with the TAK1 wild-type ... Consistently, TAK1 mutant with alanine substitution of these two residues severely inhibits IL-1 induced NFkappaB and AP-1 activities, whereas TAK1 mutant with replacement of these two sites with acidic residues slightly enhances IL-1 induced NFkappaB and AP-1 activities compared with the TAK1 wild-type
Zhao et al., Am J Respir Cell Mol Biol 2009 : Exposure of HBEpCs to PM stimulated phosphorylation of NF-kappaB and C/EBPbeta, while the NF-kappaB inhibitor, Bay11-7082, or C/EBPbeta siRNA attenuated PM-induced COX-2 expression and IL-6 release ... Exposure of HBEpCs to PM stimulated phosphorylation of NF-kappaB and C/EBPbeta, while the NF-kappaB inhibitor, Bay11-7082, or C/EBPbeta siRNA attenuated PM-induced COX-2 expression and IL-6 release
Vatsyayan et al., EMBO Rep 2008 : SUMO1 modification of NF-kappaB2/p100 is essential for stimuli induced p100 phosphorylation and processing
Choi et al., Phytother Res 2008 : Fermented guava leaf extract significantly inhibited LPS induced NF-kappaB transcriptional activity
Kim et al., Free Radic Biol Med 2008 : Lutein inhibited LPS induced NF-kappaB activation , which highly correlated with its inhibitory effect on LPS induced IkappaB kinase (IKK) activation, IkappaB degradation, nuclear translocation of NF-kappaB, and binding of NF-kappaB to the kappaB motif of the iNOS promoter ... Our data suggest that H ( 2 ) O ( 2 ) modulates IKK dependent NF-kappaB activation by promoting the redox-sensitive activation of the PI3K/PTEN/Akt and NIK/IKK pathways
Takahashi et al., Biochem Biophys Res Commun 2008 : Selective depletion of Nup88 by small interfering RNA ( siRNA ) inhibited NF-kappaB dependent reporter gene activation and the nuclear translocation of NF-kappaB without affecting the upstream activation pathway in NIH3T3 cells ... Overexpression of Nup88 in tumor cells may, thus be involved in the constitutive NF-kappaB activation
Shin et al., Int Immunopharmacol 2008 (Edema...) : LPS induced NF-kappaB activation was prevented in RAW264.7 macrophages by inhibition of I-kappaBalpha phosphorylation ... LPS induced NF-kappaB activation was prevented in RAW264.7 macrophages by inhibition of I-kappaBalpha phosphorylation
Varfolomeev et al., J Biol Chem 2008 : c-IAP1 and c-IAP2 are critical mediators of tumor necrosis factor alpha (TNFalpha) induced NF-kappaB activation ... Both c-IAP1 and c-IAP2 have been implicated in TNFalpha stimulated NF-kappaB activation ... Here we investigate the role of c-IAP1 and c-IAP2 in TNFalpha stimulated activation of NF-kappaB ... Here we investigate the role of c-IAP1 and c-IAP2 in TNFalpha stimulated activation of NF-kappaB ... Here we investigate the role of c-IAP1 and c-IAP2 in TNFalpha stimulated activation of NF-kappaB ... We demonstrate that TNFalpha induced NF-kappaB activation is severely diminished in the absence of both c-IAP proteins ... Therefore, c-IAP1 and c-IAP2 are required for TNFalpha stimulated RIP1 ubiquitination and NF-kappaB activation ... Therefore, c-IAP1 and c-IAP2 are required for TNFalpha stimulated RIP1 ubiquitination and NF-kappaB activation ... Therefore, c-IAP1 and c-IAP2 are required for TNFalpha stimulated RIP1 ubiquitination and NF-kappaB activation
Rallabhandi et al., J Biol Chem 2008 (Inflammation) : In the absence of TLR4, PAR2 induced NF-kappaB activity was inhibited by dominant negative ( DN ) -TRIF or DN-TRAM constructs, but not by DN-MyD88 , findings confirmed using cell-permeable, adapter-specific BB loop blocking peptides ... In the absence of TLR4, PAR2 induced NF-kappaB activity was inhibited by dominant negative ( DN ) -TRIF or DN-TRAM constructs, but not by DN-MyD88, findings confirmed using cell-permeable, adapter-specific BB loop blocking peptides ... Co-expression of TLR4/MD-2/CD14 with PAR2 in HEK293T cells led to a synergistic increase in AP-induced NF-kappaB signaling that was MyD88 dependent and required a functional TLR4, despite the fact that AP exhibited no TLR4 agonist activity
Huang et al., Oncogene 2008 (Neoplasms) : ABINs inhibit EGF receptor mediated NF-kappaB activation and growth of EGF receptor overexpressing tumour cells ... However, the mechanisms that regulate EGF induced NF-kappaB activation are still largely unknown ... Here we show that EGF can induce NF-kappaB dependent gene expression independently from IkappaBalpha degradation or p100 processing in EGFR overexpressing HEK293T cells ... Moreover, EGF induced NF-kappaB activation could be inhibited by overexpression of ABINs, which were previously identified as intracellular inhibitors of tumour necrosis factor, interleukin-1 and lipopolysaccharide induced NF-kappaB activation
Sun et al., Basic Res Cardiol 2009 : AGE-HSA also promoted EPCs apoptosis and inhibited EPCs migration and increased NF-kappaB activity, these effects could be significantly attenuated by the anti-RAGE neutralizing antibody as well as by p38 and ERK MAPK inhibitors ... AGE-HSA also promoted EPCs apoptosis and inhibited EPCs migration and increased NF-kappaB activity, these effects could be significantly attenuated by the anti-RAGE neutralizing antibody as well as by p38 and ERK MAPK inhibitors
Kretzmann et al., Inflamm Bowel Dis 2008 (Colitis...) : We investigated the effects of glutamine on proinflammatory gene expression and activation of nuclear factor kappa B (NF-kappaB) and signal transducers and activators of transcription ( STAT ) in a rat model of experimental colitis
Miettinen et al., J Leukoc Biol 2008 : LGG and S. pyogenes also required TLR2 for NF-kappaB activation
Sundararaj et al., J Leukoc Biol 2008 : Moreover, we showed that simvastatin inhibited LPS stimulated nuclear AP-1, but not NF-kappaB activity, and the inhibition was reversed by addition of GGPP
Solt et al., Immunol Res 2008 : We describe here how these studies have identified a unique inhibitor of pro-inflammatory NF-kappaB signaling, an unforeseen role for IKKalpha in the classical NF-kappaB pathway, and a novel functional domain in IKKbeta that is not present in IKKalpha
László et al., Photochem Photobiol 2008 : The late-phase activation of NF-kappaB occurs between 16 and 48 h post-irradiation and the mechanism is not clear due to the fact that NF-kappaB was activated in the presence of high level of IkappaBalpha ... Based upon our results, we propose a novel mechanism for translation regulated IkappaBalpha depletion and MSK mediated NF-kappaB activation at 24 h post-UV-irradiation
Lin et al., Cell Signal 2008 : These enhancement effects were LPA ( 1 ) - and LPA ( 3 ) -dependent and required cyclooxygenase-2 (COX-2) , endothelial growth factor receptor ( EGFR ) transactivation and activation of nuclear factor kappaB (NF-kappaB)
Kuo et al., Blood 2008 (Lymphoma, B-Cell, Marginal Zone...) : In B-cell lymphoma Pfeiffer cells, BAFF activated NF-kappaB and AKT ; the activated NF-kappaB up-regulated BCL10, and the activated AKT caused formation of BCL10/BCL3 complexes that translocated to the nucleus ... In B-cell lymphoma Pfeiffer cells, BAFF activated NF-kappaB and AKT ; the activated NF-kappaB up-regulated BCL10, and the activated AKT caused formation of BCL10/BCL3 complexes that translocated to the nucleus ... In B-cell lymphoma Pfeiffer cells, BAFF activated NF-kappaB and AKT ; the activated NF-kappaB up-regulated BCL10 , and the activated AKT caused formation of BCL10/BCL3 complexes that translocated to the nucleus
Song et al., J Cereb Blood Flow Metab 2008 (Disease Models, Animal...) : The role of Akt signaling in oxidative stress mediates NF-kappaB activation in mild transient focal cerebral ischemia ... Inhibition of Akt phosphorylation induced decreases in sequential NF-kappaB signaling after 30 mins of tFCI at 1 h
Jiang et al., Sichuan Da Xue Xue Bao Yi Xue Ban 2008 : To investigate the effects of N-acetylcysteine ( NAC ) on the expression of IL-8 and the activity of NF-kappaB , which are induced by lipopolysaccharide (LPS) in human uterine smooth cell ... To investigate the effects of N-acetylcysteine ( NAC ) on the expression of IL-8 and the activity of NF-kappaB , which are induced by lipopolysaccharide (LPS) in human uterine smooth cell ... NAC may inhibit the induction IL-8 gene and protein by LPS inhibiting NF-kappaB activation in human uterine smooth cell ... NAC may inhibit the induction IL-8 gene and protein by LPS inhibiting NF-kappaB activation in human uterine smooth cell
Chandrasekar et al., J Biol Chem 2008 (Inflammation) : Adiponectin blocks interleukin-18 mediated endothelial cell death via APPL1 dependent AMP activated protein kinase (AMPK) activation and IKK/NF-kappaB/PTEN suppression ... Treatment with IL-18 suppressed Akt phosphorylation and its associated kinase activity, induced IkappaB kinase (IKK)-NF-kappaB dependent PTEN activation, and promoted endothelial cell death
Doceul et al., J Gen Virol 2008 : The Npro product of classical swine fever virus interacts with IkappaBalpha , the NF-kappaB inhibitor
Reyna et al., Diabetes 2008 (Diabetes Mellitus, Type 2...) : Palmitate also increased IL-6 and SOD2 gene expression, and this effect was prevented by inhibiting NFkappaB ... Palmitate also increased IL-6 and SOD2 gene expression, and this effect was prevented by inhibiting NFkappaB
Chen et al., FEBS Lett 2008 : PP4 negatively regulated LPS induced and TRAF6 mediated NF-kappaB activation by inhibiting the ubiquitination of TRAF6 ... PP4 negatively regulated LPS induced and TRAF6 mediated NF-kappaB activation by inhibiting the ubiquitination of TRAF6
Tsoyi et al., Cell Signal 2008 : LPS induced NF-kappaB and STAT-1 activation was partially prevented by HO-1 overexpression ... LPS induced NF-kappaB and STAT-1 activation was partially prevented by HO-1 overexpression ... Furthermore, HO-1 siRNA partly reversed not only the LPS induced NF-kappaB activation and STAT-1 phosphorylation but also inhibition of these actions by CKD 712
Suzuki et al., Biomed Pharmacother 2009 : DHMEQ inhibited lipopolysaccharide (LPS) induced NF-kappaB activation
Wurfel et al., Am J Respir Crit Care Med 2008 (Genetic Predisposition to Disease...) : TLR1 ( -7202G ) marked a coding SNP that causes higher TLR1 induced NF-kappaB activation and higher cell surface TLR1 expression
Yomogida et al., Int J Mol Med 2008 : Furthermore, glucosamine inhibited the TNF-alpha induced phosphorylation of p38MAPK and NF-kappaB p65, and the nuclear translocation of NF-kappaB in the cells
Huang et al., BMC systems biology 2008 : We have used this technique to quantitatively characterize activation of the transcription factor NF-kappaB by the cytokine TNF-alpha
Karger et al., Cell Signal 2008 : CTGF stimulated binding of NF-kappaB to the IL-6 promoter, and siRNA targeting the NF-kappaB subunit RelA interfered with CTGF induced IL-6 expression, implicating the NF-kappaB pathway in the mediation of the CTGF effect
Collins-Underwood et al., Free Radic Biol Med 2008 : We report that irradiating rat brain microvascular endothelial cells in vitro leads to increased ( i ) intracellular ROS generation, ( ii ) activation of the transcription factor NFkappaB , ( iii ) expression of ICAM-1 and PAI-1, and ( iv ) expression of Nox4, p22(phox), and p47(phox) ... We report that irradiating rat brain microvascular endothelial cells in vitro leads to increased ( i ) intracellular ROS generation, ( ii ) activation of the transcription factor NFkappaB , ( iii ) expression of ICAM-1 and PAI-1, and ( iv ) expression of Nox4 , p22(phox), and p47(phox) ... We report that irradiating rat brain microvascular endothelial cells in vitro leads to increased ( i ) intracellular ROS generation, ( ii ) activation of the transcription factor NFkappaB , ( iii ) expression of ICAM-1 and PAI-1 , and ( iv ) expression of Nox4, p22(phox), and p47(phox) ... Pharmacologic and genetic inhibition of NADPH oxidase blocked the radiation mediated upregulation of intracellular ROS, activation of NFkappaB , and upregulation of ICAM-1 and PAI-1 ... Pharmacologic and genetic inhibition of NADPH oxidase blocked the radiation mediated upregulation of intracellular ROS, activation of NFkappaB , and upregulation of ICAM-1 and PAI-1 ... Pharmacologic and genetic inhibition of NADPH oxidase blocked the radiation mediated upregulation of intracellular ROS, activation of NFkappaB , and upregulation of ICAM-1 and PAI-1
Wu et al., Atherosclerosis 2009 (Atherosclerosis...) : CRH significantly up-regulated the expression of both protein and mRNA for vascular cell adhesion molecule-1 ( VCAM-1 ), together with a markedly increased activation of nuclear factor kappa B (NF-kappaB) in aortas ... Meanwhile, both the enhanced expression of VCAM-1 and increased activation of NF-kappaB induced by CRH in aortas of LDLr-/- mice were also largely suppressed by NBI27914, whereas these inhibitory effects were not observed in anti-Svg-30 group ... Meanwhile, both the enhanced expression of VCAM-1 and increased activation of NF-kappaB induced by CRH in aortas of LDLr-/- mice were also largely suppressed by NBI27914, whereas these inhibitory effects were not observed in anti-Svg-30 group ... The enhanced VCAM-1 expression which probably resulted from increased activation of NF-kappaB induced by CRH , may be one of the important molecular mechanisms by which CRH accelerates atherosclerosis
García et al., Leuk Res 2009 (Lymphoma) : PI3K/Akt inhibition modulates multidrug resistance and activates NF-kappaB in murine lymphoma cell lines ... PI3K/Akt inhibition modulates multidrug resistance and activates NF-kappaB in murine lymphoma cell lines
Park et al., J Immunol 2008 : Knockdown of PKD1 revealed that PKD1 is required for activation of NF-kappaB and MAPKs, and subsequent expression of cytokines in response to CpG-B DNA
Hervé et al., J Immunol 2008 : LPS induces the nuclear translocation of the NF-kappaB p65 subunit and the activation of three different NF-kappaB complexes in chorionic cells ... The presence of the PDE4 inhibitor rolipram reduces the TNF-alpha production and the activation of the three NF-kappaB complexes
Ermolaeva et al., Nat Immunol 2008 : Moreover, TRADD deficiency inhibited poly ( I:C ) -mediated RIP1 ubiquitination and activation of NF-kappaB and mitogen activated protein kinase signaling in fibroblasts but not in bone marrow macrophages
Funakoshi-Tago et al., Biochem Pharmacol 2008 : Celecoxib potently inhibits TNFalpha induced nuclear translocation and activation of NF-kappaB ... Here, we found that Celecoxib potently inhibited TNFalpha induced transcriptional activity and DNA binding activity of NF-kappaB ; however, Celecoxib had no effect on TNFalpha induced IKK activation and degradation of IkappaBalpha and IkappaBbeta, suggesting that it inhibited NF-kappaB activation via suppressing downstream of IKK activation and IkappaBs degradation ... Taken together, these data indicate that Celecoxib specifically inhibits TNFalpha induced NF-kappaB activation at the level of its nuclear translocation
Cao et al., Cell Signal 2008 : Inhibition of EGFR also suppresses UV-induced activation of PI3K/AKT/mTOR/S6K and NF-kappaB signal transduction pathways
Zheng et al., Infect Immun 2008 : Campylobacter induced interleukin-8 secretion in polarized human intestinal epithelial cells requires Campylobacter secreted cytolethal distending toxin- and Toll-like receptor mediated activation of NF-kappaB ... Taken together, our results demonstrate that Campylobacter induced IL-8 secretion requires functional flagella and CDT and depends on the activation of NF-kappaB through TLR signaling and CDT in human intestinal epithelial cells
Bednarski et al., Mol Cancer Ther 2008 : The absence of either IKKalpha or IKKbeta or both kinases resulted in impaired induction of NF-kappaB DNA binding activity in response to doxorubicin ... The absence of either IKKalpha or IKKbeta or both kinases resulted in impaired induction of NF-kappaB DNA binding activity in response to doxorubicin ... The inhibition of doxorubicin induced NF-kappaB activation by the knockdown of either catalytic subunit resulted in increased cleaved caspase-3 and cleaved poly ( ADP-ribose ) polymerase and increased apoptosis when compared with doxorubicin alone
Röder-Stolinski et al., Inhal Toxicol 2008 : In the presence of IKK-NBD, a specific NF-kappa B inhibitor, or the inhibitors of the p38 MAP kinase SB 203580 and SB 202190, the chlorobenzene related MCP-1 release was suppressed, suggesting an involvement of both pathways in the chlorobenzene induced expression of MCP-1
Dasu et al., Diabetes 2008 : High glucose increased TLR expression, myeloid differentiation factor 88, interleukin-1 receptor associated kinase-1, and nuclear factor-kappaB (NF-kappaB) p65 dependent activation in THP-1 cells ... High glucose increased TLR expression, myeloid differentiation factor 88, interleukin-1 receptor associated kinase-1, and nuclear factor-kappaB (NF-kappaB) p65 dependent activation in THP-1 cells ... High glucose increased TLR expression, myeloid differentiation factor 88, interleukin-1 receptor associated kinase-1 , and nuclear factor-kappaB (NF-kappaB) p65 dependent activation in THP-1 cells ... Knocking down both TLR2 and TLR4 in the cells resulted in a 76 % ( P < 0.05 ) decrease in high-glucose induced NF-kappaB activity, suggesting an additive effect ... Knocking down both TLR2 and TLR4 in the cells resulted in a 76 % ( P < 0.05 ) decrease in high-glucose induced NF-kappaB activity, suggesting an additive effect
Chen et al., Kidney Int 2008 (Hypertension...) : Candesartan suppressed TNF induced chemokine expression and NFkappaB activation independent of AT1R blockade in cultured renal tubular epithelial cells
Pan et al., J Agric Food Chem 2008 : Treatment with pterostilbene resulted in the reduction of LPS induced nuclear translocation of the nuclear factor-kappaB (NFkappaB) subunit and the dependent transcriptional activity of NFkappaB by blocking phosphorylation of inhibitor kappaB (IkappaB)alpha and p65 and subsequent degradation of IkappaB alpha ... Taken together, these results show that pterostilbene down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NFkappaB by interfering with the activation of PI3K/Akt/IKK and MAPK ... Taken together, these results show that pterostilbene down regulates inflammatory iNOS and COX-2 gene expression in macrophages by inhibiting the activation of NFkappaB by interfering with the activation of PI3K/Akt/IKK and MAPK
Liu et al., Mol Immunol 2008 : DNA binding activity of NF-kappaB and phosphorylation of p65 are induced by N-acetylcysteine through phosphatidylinositol (PI) 3-kinase ... In this study, we investigated the effect of NAC on NF-kappaB activation ... In HeLa cells, Hep3B cells, and A549 cells, DNA binding activity of NF-kappaB was induced by NAC without any other stimulation but not by tetramethylthiourea ( TMTU ) or vitamin C, suggesting that ROS is not involved in the effect of NAC ... The NAC induced DNA binding activity of NF-kappaB and phosphorylation of p65 were sensitive to a phosphatidylinositol (PI) 3-kinase inhibitor, partially sensitive to an IkappaB kinase (IKK) inhibitor, but not sensitive to a Bruton's tyrosine kinase (Btk) inhibitor
Jung et al., J Nutr Biochem 2009 : In this study, we examined ferulate for its ability to suppress redox-sensitive, proinflammatory NF-kappaB activation via NF-kappaB inducing kinase (NIK)/IkappaB kinase (IKK) and mitogen activated protein kinases ( MAPKs ) by reducing oxidative stress in aged rats
Illek et al., Am J Physiol Lung Cell Mol Physiol 2008 : Neither flagellin nor IL-1beta altered transepithelial fluxes of membrane-impermeant dextran ( 10 kDa ) or lucifer yellow ( mol wt = 457 ), but both activated p38, NF-kappaB , and IL-8 secretion ... It is concluded that : 1 ) flagellin and IL-1beta activated p38, NF-kappaB , IL-8, and CFTR dependent anion secretion without altering tight junction permeability ; 2 ) p38 played a role in regulating I ( Cl ) and IL-8 but not NF-kappaB ; and 3 ) p38 was more important in flagellin- than IL-1beta stimulated responses
Liu et al., Invest Ophthalmol Vis Sci 2008 : Poly ( I:C ) induces the up-regulation of TLR3, the MAPK dependent expression of proinflammatory cytokines, chemokines, and adhesion molecules and the activation of NF-kappaB in human corneal fibroblasts
Wang et al., Leukemia & lymphoma 2008 (Burkitt Lymphoma) : CD40L mediated inhibition of NF-kappaB in CA46 Burkitt lymphoma cells promotes apoptosis
Hýzd'alová et al., Cytokine 2008 : TNF-alpha induced rapid NF-kappaB activation in both HT-29 and FHC cell lines and this effect was differently modulated by NaBt in these two cell lines ... In HT-29 cells, NaBt potentiated NF-kappaB activity induced by TNF-alpha after 4h treatment ... During additional time of TNF-alpha and NaBt co-treatment, NaBt decreased the TNF-alpha mediated NF-kappaB activity in both cell types
Lee et al., Cytokine 2008 (Colitis...) : Furthermore, mycophenolic acid ( MPA ) reduced TNF-alpha stimulated NF-kappaB activation in HT-29 colon epithelial cells
Uffort et al., J Invest Dermatol 2009 (MAP Kinase Signaling System...) : NF-kappaB mediates mitogen activated protein kinase pathway dependent iNOS expression in human melanoma ... Two specific NF-kappaB inhibitors suppressed iNOS expression, demonstrating regulation of iNOS by NF-kappaB
Wijagkanalan et al., Mol Pharmacol 2008 (Pneumonia) : DPML significantly inhibited tumor necrosis factor alpha, interleukin-1beta , and cytokine induced neutrophil chemoattractant-1 levels, suppressed neutrophil infiltration and myeloperoxidase activity, and inhibited NFkappaB and p38 mitogen activated protein kinase activation in the lung ... DPML significantly inhibited tumor necrosis factor alpha, interleukin-1beta, and cytokine induced neutrophil chemoattractant-1 levels, suppressed neutrophil infiltration and myeloperoxidase activity, and inhibited NFkappaB and p38 mitogen activated protein kinase activation in the lung ... DPML significantly inhibited tumor necrosis factor alpha, interleukin-1beta , and cytokine induced neutrophil chemoattractant-1 levels, suppressed neutrophil infiltration and myeloperoxidase activity, and inhibited NFkappaB and p38 mitogen activated protein kinase activation in the lung
Chavey et al., Mol Pharmacol 2008 (Breast Neoplasms) : These observations are corroborated by an up-regulation of NF-kappaB activity in MCF-7 cells in the presence of TSA ... In summary, our results demonstrate that NF-kappaB pathway repression by HDAC is responsible for the low expression of IL-8 in ERalpha positive breast cancer cells ... In summary, our results demonstrate that NF-kappaB pathway repression by HDAC is responsible for the low expression of IL-8 in ERalpha positive breast cancer cells
Kogut et al., Innate Immun 2008 : Flagellin and lipopolysaccharide up-regulation of IL-6 and CXCLi2 gene expression in chicken heterophils is mediated by ERK1/2 dependent activation of AP-1 and NF-kappaB signaling pathways ... Flagellin and lipopolysaccharide up-regulation of IL-6 and CXCLi2 gene expression in chicken heterophils is mediated by ERK1/2 dependent activation of AP-1 and NF-kappaB signaling pathways ... Taken together these data demonstrate that FLG and LPS stimulate the up-regulation of expression of IL-6 and CXCLi2 through an ERK1/2 dependent activation of both NF-kappaB and AP-1
Lizundia et al., Innate Immun 2008 : However, no clear role for the RSV F protein of either human or bovine RSV alone in stimulating TLR4 dependent NF-kappaB activation was observed
Orr et al., Circ Res 2008 : p21 activated kinase signaling regulates oxidant dependent NF-kappa B activation by flow ... We now show that inhibiting PAK in cells on fibronectin blocked NF-kappaB activation by both laminar and oscillatory flow in vitro and at sites of disturbed flow in vivo ... These data demonstrate that PAK controls NF-kappaB activation by modulating the sensitivity of cells to ROS
Grosjean-Raillard et al., Apoptosis 2008 (Leukemia, Myeloid, Acute...) : Flt3 receptor inhibition reduces constitutive NFkappaB activation in high-risk myelodysplastic syndrome and acute myeloid leukemia ... High-risk myelodysplastic syndrome (MDS) and acute myeloid leukemia ( AML ) are characterized by the activation of the anti-apoptotic transcription factor NFkappaB , via the IKK complex ... Chemical inhibition or knockdown of Flt3 with small interfering RNAs reduced NFkappaB activation in MDS and AML cell lines, as well as in primary CD34 ( + ) bone marrow cells from patients, causing apoptosis
Turnbull et al., Journal of inflammation (London, England) 2008 : Here we examine their effects upon TNFalpha release from lipopolysaccharide (LPS) stimulated human monocytes and monocyte derived macrophages and investigate a potential mechanism of action through effects on LPS stimulated nuclear factor-kappa B (NF-kappaB) activation ... LPS stimulated loss of cytoplasmic IkappaBalpha and nuclear translocation of the p65 NF-kappaB subunit was inhibited by the active NO-furoxans ... LPS stimulated loss of cytoplasmic IkappaBalpha and nuclear translocation of the p65 NF-kappaB subunit was inhibited by the active NO-furoxans
Carpentier et al., Biochem Biophys Res Commun 2008 : This was associated with enhanced TNF-R2 induced JNK activation, whereas TNF-R2 induced NF-kappaB activation remained unaffected
Park et al., Cell Biol Int 2008 (Colonic Neoplasms) : To clarify the role of NF-kappaB on IL-8 expression in echinomycin mediated apoptosis of HT-29 cells, ELISA plus RT-PCR clearly showed that IL-8 production is inducible by echinomycin treatment
Rosa et al., Nitric Oxide 2008 (Osteoarthritis, Knee) : To elucidate the role of endogenous inducible nitric oxide ( NO ) on the regulation of NF-kappaB activity in human chondrocytes, we evaluated ( i ) the pattern of expression of the neuronal ( nNOS ) and inducible ( iNOS ) NO synthase isoforms and the basal NF-kappaB activity in normal and osteoarthritic ( OA ) human chondrocytes, ( ii ) the role of cytokines and growth factors in modulating the protein levels of the two NOS isoforms, and ( iii ) the effect of inhibiting endogenous inducible NO production on the ability of interleukin-1beta (IL-1) to induce NF-kappaB activation
O'Sullivan et al., J Surg Res 2009 (Inflammation) : The role of P38 MAPK and PKC in BLP induced TNF-alpha release, apoptosis, and NFkappaB activation in THP-1 monocyte cells ... The role of P38 MAPK and PKC in BLP induced TNF-alpha release, apoptosis, and NFkappaB activation in THP-1 monocyte cells ... In this study, we set out to examine the precise role of both protein kinase C ( PKC ) and P38 MAPK signaling kinases in bacterial lipoprotein (BLP) induced nuclear factor-kappa B (NFkappaB) activation and tumor necrosis factor-alpha (TNFalpha) release in THP-1 monocytic cell line ... PMA and BLP induced rapid nuclear translocation of NFkappaB , which was inhibited by pretreatment with both SB-202190 and bis I, and SB202190 but not bis I, respectively
Kondo et al., Invest Ophthalmol Vis Sci 2008 (Corneal Ulcer...) : In contrast to dexamethasone, TPCA-1 inhibited the phosphorylation and degradation of IkappaBalpha and the nuclear translocation of NF-kappaB induced by IL-1beta
Werner et al., Genes Dev 2008 : TNF induced NF-kappaB activity shows complex temporal regulation whose different phases lead to distinct gene expression programs
Chun et al., Mol Cells 2008 : TNF-alpha induced NF-kappaB activation was examined using these mutants and only zS4 was found to stimulate activation ... Collectively, the results indicate that a spliced form of ZNF268 lacking the KRAB domain is located in the cytosol, where it seems to play a role in TNF-alpha induced NF-kappaB activation by interacting with the IKK complex ... Collectively, the results indicate that a spliced form of ZNF268 lacking the KRAB domain is located in the cytosol, where it seems to play a role in TNF-alpha induced NF-kappaB activation by interacting with the IKK complex
Snoeks et al., Infect Immun 2008 : Moreover, in HT-29 cells with silenced Foxo3a, the amount of IkappaBalpha , an NF-kappaB inhibitor , is decreased
Guillot et al., Infect Immun 2008 (Cryptococcosis...) : Intracellular signaling analysis demonstrated that TNF-alpha and KC/CXCL1 production was regulated by NF-kappaB and phosphatidylinositol 3 kinase in both strains ; however, SJL/J macrophages exhibited heightened and prolonged activation in response to C. neoformans infection compared to that of C57BL/6J ... Intracellular signaling analysis demonstrated that TNF-alpha and KC/CXCL1 production was regulated by NF-kappaB and phosphatidylinositol 3 kinase in both strains ; however, SJL/J macrophages exhibited heightened and prolonged activation in response to C. neoformans infection compared to that of C57BL/6J
Roach et al., Neuroimmunomodulation 2008 (Astrocytoma...) : Although p38 and nuclear factor (NF)-kappaB are essential for the synergistic release of IL-6 , GABA did not affect either p38 phosphorylation or I kappaB-alpha degradation
Arita et al., World J Gastroenterol 2008 (Adenocarcinoma...) : To clarify whether Lysophosphatidic acid (LPA) activates the nuclear translocation of nuclear factor-kappaB (NF-kappaB) in pancreatic cancer ... LPA also caused the nuclear translocation of NF-kappaB ... The translocation of NF-kappaB was similarly attenuated by PTX and U73122, but phorbol ester, an activator of protein kinase C, alone did not translocate NF-kappaB ... Staurosporine, a protein kinase C inhibitor, attenuated translocation of NF-kappaB induced by LPA ... These findings suggest that protein kinase C is activated endogenously in Panc-1, and protein kinase C is essential for activating NF-kappaB with cytosolic calcium and that LPA induces the nuclear translocation of NF-kappaB in Panc-1 by mobilizing cytosolic free calcium
Pan et al., Mol Nutr Food Res 2008 : Treatment with 6-shogaol resulted in the reduction of LPS induced nuclear translocation of nuclear factor-kappaB (NF kappaB) subunit and the dependent transcriptional activity of NF kappaB by blocking phosphorylation of inhibitor kappaB (I kappaB)alpha and p65 and subsequent degradation of I kappaB alpha
Ohba et al., J Orthop Res 2009 (Intervertebral Disc Displacement) : TNF-alpha induced NF-kappaB signaling reverses age related declines in VEGF induction and angiogenic activity in intervertebral disc tissues ... NF-kappaB pathway inhibitors also strongly suppressed VEGF induction
Moon et al., Int Immunopharmacol 2008 : Saucerneol D, a naturally occurring sesquilignan, inhibits LPS induced iNOS expression in RAW264.7 cells by blocking NF-kappaB and MAPK activation ... These findings suggest that SD may inhibit LPS induced iNOS expression by blocking NF-kappaB and MAPK activation
Han et al., Gut 2009 (Colitis) : This was associated with upregulation of CEC Toll-like receptor 2 expression and NF-kappaB activation ... STAT5b knockdown in T84 CEC increased TNFalpha dependent NF-kappaB and caspase-3 activation
Gobejishvili et al., Am J Physiol Gastrointest Liver Physiol 2008 (Hepatitis, Alcoholic) : We have previously shown that monocytes from patients with alcoholic hepatitis show increased constitutive and LPS induced NF-kappaB activation and TNF-alpha production ... Our recent studies showed that chronic ethanol exposure significantly decreased cellular cAMP levels in both LPS stimulated and unstimulated monocytes and Kupffer cells, leading to an increase in LPS-inducible TNF-alpha production by affecting NF-kappaB activation and induction of TNF mRNA expression
Zhang et al., Vasc Med 2008 : Our findings indicate that Ang II-induced BMP-2 expression might contribute to NF-kappaB activation
Ahn et al., Int J Cancer 2008 (Breast Neoplasms...) : Together, our results indicate that simvastatin inhibits the RANKL induced NF-kappaB activation pathway that leads to suppression of osteoclastogenesis induced by RANKL and by tumor cells, thereby suggesting its therapeutic potential in osteoporosis and in cancer related bone loss
Mandrekar et al., J Leukoc Biol 2008 (Inflammation) : Heat shock proteins ( hsps ) and the heat shock transcription factor-1 ( HSF-1 ) induced by oxidative stress regulate NF-kappaB activation and TNF-alpha gene expression in monocytes and macrophages ... Heat shock proteins ( hsps ) and the heat shock transcription factor-1 ( HSF-1 ) induced by oxidative stress regulate NF-kappaB activation and TNF-alpha gene expression in monocytes and macrophages ... Inhibition of hsp90 using geldanamycin prevented prolonged alcohol induced elevation in LPS induced NF-kappaB and TNF-alpha production
Pispa et al., Hum Mol Genet 2008 : Assessment of NF-kappaB activity with a transgenic reporter construct indicated that Eda is the main activator of NF-kappaB signalling in developing skin appendages and surprisingly that the functional overlap of Troy and Eda signalling pathways is mediated by NF-kappaB independent pathways
Prasad et al., Journal of inflammation (London, England) 2008 : TNFalpha is one of the major proinflammatory cytokines that causes endothelial dysfunction by various mechanisms including activation of transcription factor NF-kappaB , a key transcription factor that regulates expression of CAMs
Leghmari et al., Cell Immunol 2008 : Using chemical inhibitors we show that ( i ) both ERK1/2 MAP kinase and NF-kappaB transcription factor play an important role in IL-10 and TNF-alpha production, in macrophages stimulated by Tat ... Using chemical inhibitors we show that ( i ) both ERK1/2 MAP kinase and NF-kappaB transcription factor play an important role in IL-10 and TNF-alpha production, in macrophages stimulated by Tat
Schnabl et al., Biochem Biophys Res Commun 2008 (Liver Cirrhosis) : Incubation of human HSCs with a fusion protein between the extracellular domain of TLR4 and MD2 which binds LPS inhibited LPS induced NFkappaB and JNK activation
Moschonas et al., Mol Cell Biol 2008 (Neoplasms) : We show that unlike interferon signaling, which triggers the STAT1 mediated transcriptional activation of IRF-1, the ligation of CD40 in carcinomas induces the rapid upregulation of IRF-1 in a STAT1 independent but NF-kappaB dependent manner ... We show that unlike interferon signaling, which triggers the STAT1 mediated transcriptional activation of IRF-1, the ligation of CD40 in carcinomas induces the rapid upregulation of IRF-1 in a STAT1 independent but NF-kappaB dependent manner
Kim et al., J Pharmacol Exp Ther 2008 : Moreover, diarctigenin suppressed expression vector NF-kappaB p65 elicited NF-kappaB activation and also iNOS promoter activity, indicating that the compound could directly target an NF-kappa activating signal cascade downstream of IkappaB degradation and inhibit NF-kappaB regulated iNOS expression
Mahoney et al., Proc Natl Acad Sci U S A 2008 : Both cIAP1 and cIAP2 regulate TNFalpha mediated NF-kappaB activation ... Both cIAP1 and cIAP2 regulate TNFalpha mediated NF-kappaB activation ... Both cIAP1 and cIAP2 regulate TNFalpha mediated NF-kappaB activation ... Whereas NF-kappaB was properly activated by TNFalpha in cultured and primary cells deficient in either cIAP1 or 2, removal of both cIAPs severely blunted its activation ... Collectively, these data demonstrate that either cIAP1 or 2 is required for proper Rip1 polyubiquitination and NF-kappaB activation upon TNFalpha treatment
Hodgkinson et al., Pharmacogenet Genomics 2008 : In cells expressing the 299D-399T TLR4, LPS activated the transcription factor NFkappaB and increased the expression of interleukin-6 and tumor necrosis factor-alpha, and these effects were reduced by pretreatment of the cells with pravastatin or simvastatin ... LPS induced NFkappaB activation and interleukin-6 and tumor necrosis factor-alpha expression were substantially reduced in cell expressing the 299G-399T or 299D-399I variant, and undetectable in cells expressing the 299G-399I TLR4
Kim et al., Int J Mol Med 2008 : The protective effect of RCE was further demonstrated by the observed suppression of NF-kappaB dependent iNOS expression and normal insulin secreting responses to glucose in cytokines treated islets
Sugiyama et al., Eur J Pharmacol 2008 (Shock, Septic) : A peptide ( STM28 ) isolated from the screen inhibited LPS induced nuclear factor-kappaB (NF-kappaB) activation in human and mouse macrophage cells and interacted with TLR4 in yeast and mammalian cells ... STM28 showed no inhibitory effects against NF-kappaB activation induced by TLR1/2 , TLR3 and TLR9 ligands in a mouse macrophage cell line, RAW 264 ... STM28 showed no inhibitory effects against NF-kappaB activation induced by TLR1/2, TLR3 and TLR9 ligands in a mouse macrophage cell line, RAW 264
Yoon et al., J Agric Food Chem 2008 (Breast Neoplasms) : Effect of 2alpha-hydroxyursolic acid on NF-kappaB activation induced by TNF-alpha in human breast cancer MCF-7 cells
Jin et al., Cancer Res 2008 (Carcinoma, Neuroendocrine...) : In addition, the continuous activation of NF-kappaB signaling in vivo by the absence of the IkappaBalpha inhibitor prevents regression of the prostate after castration by sustaining high levels of nuclear AR and maintaining differentiated function and continued proliferation of the epithelium
Mir et al., J Neuroimmunol 2008 : Importantly, IFN-gamma induced NF-kappa B binding to DNA was totally dependent on the endogenous TNF-alpha released via MEK/ERK signalling pathway ... Thus, exposure of BV-2 cells to IFN-gamma in the presence of the selective MEK inhibitor U0126 or a neutralizing anti-TNF-alpha antibody significantly reduced IFN-gamma dependent NF-kappa B activation and iNOs expression ... In addition, by activating the Jak/STAT pathway IFN-gamma potentiated TNF-alpha induced NF-kappa B binding to DNA and activated additional transcription factors ( i.e. IRF-1 ) known to be essential for iNOs gene expression
Rodrigues et al., Biol Reprod 2008 : NFKB activation by LPS in caput epididymidis was abrogated when rats were pretreated with the NFKB inhibitor PDTC, confirming the specificity of this response
Hasler et al., J Biol Chem 2008 (Inflammation) : Using immortalized collecting duct principal cells ( mpkCCDcl4 ), we found that, acting independently of vasopressin, activation of NF-kappaB by lipopolysaccharide (LPS) decreased AQP2 mRNA and protein levels in a time- and dose dependent manner but did not decrease AQP2 mRNA stability
Hattori et al., Cardiovasc Res 2009 : Cilostazol also dose-dependently inhibited tumour necrosis factor alpha (TNFalpha) induced NF-kappaB activation and TNFalpha induced I kappa B kinase activity
Frasor et al., Endocrinology 2008 : Surprisingly, the combination of E2 and cytokines leads to a synergistic up-regulation of PTGES in an ER and nuclear factor-kappaB (NFkappaB) dependent manner
Bhat et al., FEBS Lett 2008 (Glioma) : Selective repression of YKL-40 by NF-kappaB in glioma cell lines involves recruitment of histone deacetylase-1 and -2
Luo et al., Toxicol Appl Pharmacol 2008 : Furthermore, IL-1beta induced cPLA2 expression and PGE2 synthesis was inhibited by a selective NF-kappaB inhibitor ( helenalin ) or transfection with dominant negative mutants of NF-kappaB inducing kinase (NIK), IkappaB kinase (IKK)-alpha, and IKK-beta ... Consistently, IL-1beta stimulated both IkappaB-alpha degradation and NF-kappaB translocation into nucleus in these cells ... Consistently, IL-1beta stimulated both IkappaB-alpha degradation and NF-kappaB translocation into nucleus in these cells
Markovina et al., Mol Cancer Res 2008 (Multiple Myeloma) : Further elucidation of the mechanism of PIR NF-kappaB regulation could lead to the identification of novel diagnostic biomarkers and/or therapeutic targets for MM treatment
Sharma et al., Int J Cancer 2008 (Glioblastoma) : Ebselen ( 2-phenyl-1,2-benzisoselenazol-3 [ 2H ] one ) -a selenoorganic compound is known to prevent TNFalpha mediated NF-kappaB activity ... Sensitization of TNFalpha activated glioma cells to apoptosis by Ebselen involved 2 pathways : ( i ) abrogation of TNFalpha induced NF-kappaB activation and ( ii ) induction of Fas associated death inducing signaling complex ( DISC ) formation
Itatsu et al., Liver Int 2009 (Cholangiocarcinoma) : As for the signalling pathway, TNF-alpha stimulation led to the phosphorylation of extracellular signal regulated kinase 1/2 ( Erk1/2 ) and p38 mitogen activated protein kinase ( p38MAPK ) and translocation of nuclear factor kappaB (NF-kappaB) ( p65 ) into the nuclei ... These data suggest that TNF-alpha/TNF-R1 interaction leads to the phosphorylation of Erk1/2 and p38MAPK and nuclear translocation of NF-kappaB , which is closely associated with the production and activation of MMP-9 in cultured CC cells of HuCTT-1 and CCKS-1 ... These data suggest that TNF-alpha/TNF-R1 interaction leads to the phosphorylation of Erk1/2 and p38MAPK and nuclear translocation of NF-kappaB , which is closely associated with the production and activation of MMP-9 in cultured CC cells of HuCTT-1 and CCKS-1
Veillat et al., Fertil Steril 2009 (Endometriosis...) : To investigate the involvement of the nuclear factor (NF)-kappaB in the interleukin (IL)-1 beta mediated macrophage migration inhibitory factor ( MIF ) gene activation ... This study showed a significant dose dependent increase of MIF protein secretion and mRNA expression, the NF-kappaB translocation into the nucleus, I kappaB phosphorylation, I kappaB degradation, and human MIF promoter activity in endometriotic stromal cells in response to IL-1 beta ... This study suggests the involvement of the nuclear transcription factor NF-kappaB in MIF gene activation in ectopic endometrial cells in response to IL-1 beta and identifies a possible pathway of endometriosis associated inflammation and ectopic cell growth
Dwyer et al., Hypertens Res 2008 (Disease Models, Animal...) : Our data suggest a role for the Ras/mitogen activated protein kinase ( MAPK ) signaling pathway and the tumor necrosis factor (TNF) receptor mediated activation of nuclear factor-kappaB (NF-kappaB) in the etiology of cardiac enlargement in the HHR
Royuela et al., Histol Histopathol 2008 (Prostatic Neoplasms) : TNFalpha also exerts proliferation by p38 activation, or when TRAF-2 simultaneously induces the transcription factor NF-kappaB by NIK
Kieser et al., Biol Chem 2008 : TRADD mediates TNFR1 activation of NF-kappaB and c-Jun N-terminal kinase (JNK), as well as caspase dependent apoptosis ... TRADD mediates TNFR1 activation of NF-kappaB and c-Jun N-terminal kinase (JNK), as well as caspase dependent apoptosis ... The unique structure of the LMP1-TRADD complex dictates an unusual type of TRADD dependent NF-kappaB signaling and subverts TRADD 's potential to induce apoptosis
Dagvadorj et al., Innate Immun 2008 : IL-10 inhibited the activation of nuclear factor (NF)-kappaB , p38 and stress activated protein kinase ( SAPK ) in LPS stimulated RAW 264.7 cells
Clarke et al., J Immunol 2008 : In this study, we describe a novel role for protein kinase C ( PKC ) betaIotaIota in augmenting NF-kappaB mediated TNF-alpha induced transcription of the target gene CCL11 in human airway smooth muscle cells by phosphorylating the HAT p/CAF ... Studies using reporters, overexpression strategies, kinase-dead and HAT-defective mutants, and chromatin immunoprecipitation showed that PKCbetaII activation was not involved in NF-kappaB translocation, but facilitated NF-kappaB mediated CCL11 transcription by colocalizing with and phosphorylating p/CAF, and thereby acetylating histone H4 and promoting p65 association with the CCL11 promoter ... Furthermore, mouse embryonic fibroblasts from PKCbeta knockout mice showed markedly reduced TNF-alpha induced CCL11 expression and NF-kappaB reporter activity that was restored on PKCbetaII overexpression, suggesting a critical role for this pathway ... These data suggest a novel important biological role for PKCbetaIotaIota in NF-kappaB mediated CCL11 transcription by p/CAF activation and histone H4 acetylation
Króliczak et al., Acta biochimica Polonica 2008 (Neoplasms) : Sp1 and NFkappaB binding to the probes resembling their putative binding sites present in the S100A6 promoter was decreased in the presence of wild type p53
Luthra et al., J Virol 2008 : AKT1 dependent activation of NF-kappaB by the L protein of parainfluenza virus 5 ... The activation of NF-kappaB by L requires AKT1 , a serine/threonine kinase, since AKT1 small interfering RNA, an AKT inhibitor as well as a dominant negative mutant of AKT1, blocks this activation
Hayashi et al., J Neurosci 2008 (Memory Disorders...) : We herein report that an overexpression of TFAM in HeLa cells significantly inhibited rotenone induced mitochondrial ROS generation and the subsequent NF-kappaB ( nuclear factor-kappaB ) nuclear translocation
Gurzov et al., PloS one 2008 : The cytokines IL-1beta+IFN-gamma induced an early and transitory upregulation of JunB by NF-kappaB activation
Pacheco-Palencia et al., J Agric Food Chem 2008 (Skin Diseases) : Pomegranate extract ( PE ), in a range from 5 to 60 mg/L, was effective at protecting human skin fibroblasts from cell death following UV exposure, likely related to a reduced activation of the pro-inflammatory transcription factor NF-kappaB , a downregulation of proapoptotic caspase-3 , and an increased G0/G1 phase, associated with DNA repair
Talmor et al., Eur J Clin Invest 2008 (Inflammation) : Furthermore, the LPS induced nuclear factor kappaB (NFkappaB)-p65 DNA binding activity was also decreased by calcitriol
Bhattacharyya et al., Am J Physiol Gastrointest Liver Physiol 2008 : In colonic epithelium, one of the pathways of lipopolysaccharide (LPS) activation of NF-kappaB and IL-8 is via Toll-like receptor (TLR)4, MyD88, IRAK1/4, and B-cell CLL/lymphoma 10 (Bcl10)
Buzzelli et al., Endocrinology 2008 : We conclude the inhibitory effects of TNF on GH-inducible promoter activity are mediated by NFkappaB , especially p65, by a mechanism that does not require protein synthesis
Chang et al., Invest Ophthalmol Vis Sci 2008 (Uveitis, Anterior) : The effect of GS on LPS induced NF-kappaB activation in RAW cells was also examined ... In LPS stimulated macrophage RAW cell culture, GS inhibited the production of NO and PG-E2, the expression of iNOS and COX-2, and the activation of NF-kappaB ... In LPS stimulated macrophage RAW cell culture, GS inhibited the production of NO and PG-E2, the expression of iNOS and COX-2 , and the activation of NF-kappaB ... GS suppresses EIU in rats by blockading the NF-kappaB dependent signaling pathway and the subsequent production of ICAM-1 and proinflammatory mediators
Chen et al., Proc Natl Acad Sci U S A 2008 (Inflammation) : In cells from TRADD-deficient mice, TNFalpha mediated apoptosis and TNFalpha stimulated NF-kappaB , JNK, and ERK activation are defective
Seppänen et al., Acta Obstet Gynecol Scand 2008 (Carcinoma, Endometrioid...) : Activation of AP-1 seems to be involved in the growth regulating processes induced by IFN-gamma, TGF-beta(1) and TNF-alpha ; IFN-gamma is also able to increase the binding activity of NF-kappaB and inhibited apoptosis in ovarian cancer cells
Tozawa et al., Int J Urol 2008 : Furthermore, we investigated the inhibitory effect of N-acetyl-L-cysteine (NAC) on NF-kappaB activation in the human renal tubular cell line
Satta et al., Mol Immunol 2008 (Inflammation) : By using specific signalling pathway inhibitors we established that the induction of TLR2 by inflammatory stimuli was dependent on NF-kappaB , p38-MAP kinase and c-Jun kinase
Newton et al., Cell 2008 : We use these antibodies to demonstrate that RIP1, which is essential for tumor necrosis factor induced NF-kappaB activation, and IRAK1, which participates in signaling by interleukin-1beta and Toll-like receptors, both undergo polyubiquitin editing in stimulated cells
Song et al., J Crit Care 2008 : Endotoxin tolerance and HS appear to share a common immune suppressive effect, possibly through HSF-1 mediated competitive inhibition of NF-kappaB nuclear binding
Qi et al., Sci China C Life Sci 2000 : EMSA showed that LPS could augment the NF-kappaB binding activity to p40 promoter DNA ... However, IFN-gamma could neither enhance the LPS induced NF-kappaB activity nor promote the degradation of IkappaBalpha
Lu et al., Cell Mol Neurobiol 2009 : In order to determine the possible effects of hemolysate on brain microvascular endothelial cells ( BMECs ), we examined the effects of hemolysate on the expression of intercellular adhesion molecule-1 ( ICAM-1 ) and monocyte chemoattractant protein-1 ( MCP-1 ), generation of reactive oxygen species ( ROS ), and NF-kappaB activation in rat BMECs ... In order to determine the possible effects of hemolysate on brain microvascular endothelial cells ( BMECs ), we examined the effects of hemolysate on the expression of intercellular adhesion molecule-1 ( ICAM-1 ) and monocyte chemoattractant protein-1 ( MCP-1 ), generation of reactive oxygen species ( ROS ), and NF-kappaB activation in rat BMECs
Chen et al., J Biol Chem 2008 : The lack of PLCgamma2 markedly diminished RANKL induced activation of NF-kappaB , AP-1, and NFATc1 ... The lack of PLCgamma2 markedly diminished RANKL induced activation of NF-kappaB , AP-1, and NFATc1
Thapa et al., J Pharmacol Exp Ther 2008 (Colitis) : In a cellular model of colitis using tumor necrosis factor (TNF)-alpha stimulated HT29 colon epithelial cells, treatment with CLT significantly suppressed TNF-alpha mediated IL-8 induction and NF-kappaB transcriptional activity revealed by a luciferase reporter gene assay
Yun et al., Mol Carcinog 2009 (Colonic Neoplasms) : The immunoblot, ELISA and EMSA analysis demonstrated that the treatment of HCT116 cells with delphinidin resulted in the inhibition of ( i ) IKKalpha, ( ii ) phosphorylation and degradation of IkappaBalpha, ( iii ) phosphorylation of NF-kappaB/p65 at Ser ( 536 ), ( iv ) nuclear translocation of NF-kappaB/p65, ( v ) NF-kappaB/p65 DNA binding activity, and ( vi ) transcriptional activation of NF-kappaB ... The immunoblot, ELISA and EMSA analysis demonstrated that the treatment of HCT116 cells with delphinidin resulted in the inhibition of ( i ) IKKalpha, ( ii ) phosphorylation and degradation of IkappaBalpha, ( iii ) phosphorylation of NF-kappaB/p65 at Ser ( 536 ), ( iv ) nuclear translocation of NF-kappaB/p65, ( v ) NF-kappaB/p65 DNA binding activity, and ( vi ) transcriptional activation of NF-kappaB ... The immunoblot, ELISA and EMSA analysis demonstrated that the treatment of HCT116 cells with delphinidin resulted in the inhibition of ( i ) IKKalpha, ( ii ) phosphorylation and degradation of IkappaBalpha , ( iii ) phosphorylation of NF-kappaB/p65 at Ser ( 536 ), ( iv ) nuclear translocation of NF-kappaB/p65, ( v ) NF-kappaB/p65 DNA binding activity, and ( vi ) transcriptional activation of NF-kappaB
Korashy et al., J Biochem Mol Toxicol 2008 (MAP Kinase Signaling System) : In the current study, we examined the role of redox-sensitive transcription factors, NF-kappaB and AP-1 signaling pathways in the modulation of Nqo1 by heavy metals ... These results clearly demonstrate that activation of NF-kappaB negatively regulates the expression of Nqo1 by heavy metals, whereas AP-1 signaling pathways differentially modulates the heavy metal mediated effects
Alvarez et al., Virology 2008 : Overexpression of IkappaBalpha completely abrogated COX-2 induction, and transfection of p65/relA NF-kappaB induced COX-2 transcription ... Overexpression of IkappaBalpha completely abrogated COX-2 induction, and transfection of p65/relA NF-kappaB induced COX-2 transcription
Tsoporis et al., J Biol Chem 2008 (Inflammation) : Tumor necrosis factor-alpha induced the maximal S100A6 promoter and transcription factor NF-kappaB ( p65 subunit ) ... These results demonstrate that S100A6 is induced by tumor necrosis factor-alpha via an NF-kappaB dependent mechanism, serving a role in homeostasis to limit tumor necrosis factor-alpha induced apoptosis by regulating p53 phosphorylation ... These results demonstrate that S100A6 is induced by tumor necrosis factor-alpha via an NF-kappaB dependent mechanism, serving a role in homeostasis to limit tumor necrosis factor-alpha induced apoptosis by regulating p53 phosphorylation ... These results demonstrate that S100A6 is induced by tumor necrosis factor-alpha via an NF-kappaB dependent mechanism, serving a role in homeostasis to limit tumor necrosis factor-alpha induced apoptosis by regulating p53 phosphorylation
Boisvert et al., Virology 2008 : The involvement of NF-kappaB in the PGJ ( 2 ) -dependent down-regulation of HIV-1 transcription was further assessed using the kappaB regulated luciferase encoding vectors
Oudijk et al., Mol Immunol 2008 : TNFalpha induced expression of CD83 in leukocytes is mediated by NF-kappab
Medoff et al., Am J Respir Cell Mol Biol 2009 (Asthma) : Here we demonstrate that CARMA3 ( CARD10 ) is specifically expressed in human airway epithelial cells, and that expression of CARMA3 in these cells leads to activation of NF-kappaB ... We then show that inhibition of CARMA3 activity in airway epithelial cells reduces LPA mediated NF-kappaB activity and the production of TSLP and CCL20 ... We then show that inhibition of CARMA3 activity in airway epithelial cells reduces LPA mediated NF-kappaB activity and the production of TSLP and CCL20 ... In conclusion, these data demonstrate that LPA stimulates TSLP and CCL20 expression in bronchial epithelial cells via CARMA3 mediated NF-kappaB activation
Shair et al., Cancer Res 2008 : However, constitutive activation of Akt alone did not alter cell growth, suggesting that both PI3K/Akt and NF-kappaB activation are required by LMP1
Cates et al., Comp Immunol Microbiol Infect Dis 2009 (Gram-Negative Bacterial Infections...) : To assess the role of the bovine orthologs of these proteins in bovine TLR-4 signaling, dominant negative constructs were expressed in bovine EC, and LPS induced NF-kappaB activation and apoptosis evaluated
Maelfait et al., Biochem Pharmacol 2008 : Here we review the role of caspase-8 in embryonic development, monocyte differentiation, T and B cell proliferation, and the activation of NF-kappaB
Gao et al., Lab Invest 2008 (Disease Models, Animal...) : Kallistatin promoted survival of cardiomyocytes subjected to H ( 2 ) O ( 2 ) treatment, and inhibited H ( 2 ) O ( 2 ) -induced Akt and ERK phosphorylation, as well as NF-kappaB activation
Zhou et al., World J Gastroenterol 2008 (Adenocarcinoma...) : Viable LBG or LBG- ( s ) pretreatment attenuated the expression of TLR4, inhibited the phosphorylation of TAK1 and p38MAPK, prevented the activation of NF-kappaB , and consequently blocked IL-8 production ... Viable LBG or LBG- ( s ) pretreatment attenuated the expression of TLR4, inhibited the phosphorylation of TAK1 and p38MAPK , prevented the activation of NF-kappaB , and consequently blocked IL-8 production ... Viable LBG or LBG- ( s ) pretreatment attenuated the expression of TLR4, inhibited the phosphorylation of TAK1 and p38MAPK , prevented the activation of NF-kappaB , and consequently blocked IL-8 production
Golovine et al., Clin Cancer Res 2008 (Neoplasm Invasiveness...) : In this study, we investigate the functional effect of hZIP1 overexpression on NF-kappaB activity and tumorigenic potential in human prostate cancer cells in vitro and in vivo
Bhowmick et al., Infect Immun 2008 (Cholera) : The upregulation of MUC2 and MUC5AC genes was dependent on NF-kappaB nuclear translocation
Piccagli et al., BMC structural biology 2008 : In this respect, this paper represents the first example of successfully individuation through molecular docking simulations of a promising lead compound for the inhibition of NF-kappaB-p50 biological activity and modulation of the expression of the NF-kB regulated IL8 gene
El Kihel et al., Bioorg Med Chem 2008 (Colonic Neoplasms...) : This activity was associated with Mcl-1 and PARP-1 cleavage, inhibition of NFkappaB signaling, and DNA fragmentation, demonstrating an apoptotic cell death signaling pathway ... This activity was associated with Mcl-1 and PARP-1 cleavage, inhibition of NFkappaB signaling, and DNA fragmentation, demonstrating an apoptotic cell death signaling pathway
Phulwani et al., J Immunol 2008 : In addition, NF-kappaB inhibitors attenuated TNF-alpha induced TLR2 expression in astrocytes
Zhang et al., J Immunol 2008 (Bacteremia...) : Inhibition of H ( 2 ) S formation by PAG significantly reduced the phosphorylation of ERK1/2 in lung and liver 4 h after CLP, coupled with decreased degradation of IkappaBalpha and activation of NF-kappaB
Kim et al., J Immunol 2008 (Bacterial Infections...) : Consistently, Nod2-null macrophages tolerant to LPS and MDP showed enhanced production of TNF-alpha and IL-6 as well as increased NF-kappaB and MAPK activation in response to the dipeptide KF1B, the Nod1 agonist ... Consistently, Nod2-null macrophages tolerant to LPS and MDP showed enhanced production of TNF-alpha and IL-6 as well as increased NF-kappaB and MAPK activation in response to the dipeptide KF1B , the Nod1 agonist ... Consistently, Nod2-null macrophages tolerant to LPS and MDP showed enhanced production of TNF-alpha and IL-6 as well as increased NF-kappaB and MAPK activation in response to the dipeptide KF1B, the Nod1 agonist
Martins et al., Cell Physiol Biochem 2008 : Insulin suppresses LPS induced iNOS and COX-2 expression and NF-kappaB activation in alveolar macrophages ... In the present study, we investigated the effect of insulin on the LPS induced production of nitric oxide ( NO ) and prostaglandin ( PG ) -E ( 2 ), on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 , and on nuclear factor kappa B (NF-kappaB) activation in AMs ... In the present study, we investigated the effect of insulin on the LPS induced production of nitric oxide ( NO ) and prostaglandin ( PG ) -E ( 2 ), on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and on nuclear factor kappa B (NF-kappaB) activation in AMs ... In the present study, we investigated the effect of insulin on the LPS induced production of nitric oxide ( NO ) and prostaglandin ( PG ) -E ( 2 ), on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and on nuclear factor kappa B (NF-kappaB) activation in AMs ... In the present study, we investigated the effect of insulin on the LPS induced production of nitric oxide ( NO ) and prostaglandin ( PG ) -E ( 2 ), on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 , and on nuclear factor kappa B (NF-kappaB) activation in AMs ... In the present study, we investigated the effect of insulin on the LPS induced production of nitric oxide ( NO ) and prostaglandin ( PG ) -E ( 2 ), on the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2, and on nuclear factor kappa B (NF-kappaB) activation in AMs ... These results show that in AMs stimulated by LPS, insulin prevents nuclear translocation of NF-kappaB , possibly by blocking I-kappaBalpha degradation, and supresses the production of NO and PGE ( 2 ), two molecules that contribute to septic shock
Krejsgaard et al., Leukemia 2008 (Sezary Syndrome...) : In contrast to wt FOXP3, the low molecular splice forms of FOXP3 have no inhibitory effect on nuclear factor-kappaB (NF-kappaB) activity in reporter assays which is in keeping with a constitutive NF-kappaB activity in the malignant T cells
Bauler et al., PLoS Pathog 2008 (Listeriosis) : XIAP enhanced NF-kappaB activation upon L. monocytogenes infection of activated macrophages, and prolonged phosphorylation of Jun N-terminal kinase (JNK) specifically in response to cytosolic bacteria
Ogunwobi et al., Mol Cell Endocrinol 2008 (Adenocarcinoma...) : Glycine extended gastrin stimulates proliferation via JAK2- and Akt dependent NF-kappaB activation in Barrett 's oesophageal adenocarcinoma cells ... G-Gly increased COX-2 promoter transcription in an Akt and NF-kappaB dependent manner and also reduced COX-2 mRNA degradation in an Akt-insensitive manner
Kasperczyk et al., FASEB J 2009 (Neoplasms) : To explore mechanisms controlling sonic hedgehog (Shh) expression in human cancers, we investigated regulation of Shh by the transcription factor NF-kappaB ... Further, NF-kappaB activation by tumor necrosis factor alpha (TNF-alpha) or p65 overexpression stimulates Shh promoter activity and p65 binds to Shh promoter in vivo ... Further, NF-kappaB activation by tumor necrosis factor alpha (TNF-alpha) or p65 overexpression stimulates Shh promoter activity and p65 binds to Shh promoter in vivo ... Specific NF-kappaB inhibition by inhibitory NF-kappaBalpha ( Ikappa-Balpha ) superrepressor or p65 knockdown inhibits NF-kappaB induced Shh promoter activation and Shh expression ... NF-kappaB mediated Shh expression promotes proliferation and confers resistance to TRAIL induced apoptosis
Saha et al., Am J Physiol Gastrointest Liver Physiol 2008 : Because IL-1beta also activates NF-kappaB signaling, we investigated disparate HKalpha regulation by H. pylori and IL-1beta, testing the hypothesis that IL-1beta induced HKalpha promoter activation is mediated by the transcription factor Sp1
Nozell et al., Mol Cell Biol 2008 (Glioma) : We demonstrate that an ING4 and NF-kappaB interaction exists but does not prevent NF-kappaB activation, nuclear translocation, or DNA binding ... Instead, ING4 and NF-kappaB bind simultaneously at NF-kappaB regulated promoters, and this binding correlates with reductions in p65 phosphorylation, p300, and the levels of acetylated histones and H3-Me3K4 , while enhancing the levels of HDAC-1 at these promoters
Kim et al., Exp Mol Med 2008 (Asthma...) : The stimulation of lymphoid cells with TNF-alpha , IL-1beta, and LPS resulted in significant ROS production and NF-kappaB activation ... The stimulation of lymphoid cells with TNF-alpha, IL-1beta , and LPS resulted in significant ROS production and NF-kappaB activation ... The stimulation of lymphoid cells with TNF-alpha, IL-1beta , and LPS resulted in significant ROS production and NF-kappaB activation ... The stimulation of lymphoid cells with TNF-alpha, IL-1beta, and LPS resulted in significant ROS production and NF-kappaB activation
Zeng et al., Expert Opin Ther Targets 2008 (Cell Transformation, Neoplastic...) : In mammals, RKIP regulates activation of MAPK, NF-kappaB and G protein coupled receptors ( GPCRs )
Chen et al., Zhong Xi Yi Jie He Xue Bao 2008 : To investigate the effects of tetrandrine ( Tet ) on nuclear factor kappaB (NF-kappaB) expression in leukemia cell line K562 and multidrug-resistant K562/A02 cell line ... The activations of NF-kappaB in K562 and K562/A02 cells and the effects of 1 micromol/L Tet on NF-kappaB expression were determined by immunocytochemistry and Western blot assay
Hosotani et al., J Dermatol 2008 : Exogenous IL-18 augmented phosphorylation of Akt and activation of NF-kappaB ... Exogenous IL-18 augmented phosphorylation of Akt and activation of NF-kappaB
Kustermans et al., Biochem Pharmacol 2008 : Actin cytoskeleton differentially modulates NF-kappaB mediated IL-8 expression in myelomonocytic cells
Blanchette et al., Immunology 2009 (MAP Kinase Signaling System...) : The activation of AP-1 , and not nuclear factor-kappaB (NF-kappaB) or signal transducer and activator of transcription-1 alpha ( STAT-1 alpha ), may explain the enhanced NO generation in SHP-1-deficient cells
Goldsmith et al., Immunology 2009 : Finally, we show that, while PCERA-1 does not block activation of nuclear factor (NF)-kappaB and mitogen activated protein kinases by LPS , it elevates the intracellular cAMP level
Fraczek et al., J Biol Chem 2008 : The kinase activity of IL-1 receptor associated kinase 4 is required for interleukin-1 receptor/toll-like receptor induced TAK1 dependent NFkappaB activation ... Two parallel interleukin-1 (IL-1) mediated signaling pathways have been uncovered for IL-1R-TLR mediated NFkappaB activation : TAK1 dependent and MEKK3 dependent pathways, respectively ... Two parallel interleukin-1 (IL-1) mediated signaling pathways have been uncovered for IL-1R-TLR mediated NFkappaB activation : TAK1 dependent and MEKK3 dependent pathways, respectively ... The TAK1 dependent pathway leads to IKKalpha/beta phosphorylation and IKKbeta activation, resulting in classic NFkappaB activation through IkappaBalpha phosphorylation and degradation ... IRAK4 kinase-inactive mutant failed to mediate the IL-1R-TLR induced TAK1 dependent NFkappaB activation pathway, but mediated IL-1 induced TAK1 independent NFkappaB activation and retained the ability to activate substantial gene expression, indicating a structural role of IRAK4 in mediating this alternative NFkappaB activation pathway ... IRAK4 kinase-inactive mutant failed to mediate the IL-1R-TLR induced TAK1 dependent NFkappaB activation pathway, but mediated IL-1 induced TAK1 independent NFkappaB activation and retained the ability to activate substantial gene expression, indicating a structural role of IRAK4 in mediating this alternative NFkappaB activation pathway ... Deletion analysis of IRAK4 indicates the essential structural role of the IRAK4 death domain in receptor proximal signaling for mediating IL-1R-TLR induced NFkappaB activation ... Deletion analysis of IRAK4 indicates the essential structural role of the IRAK4 death domain in receptor proximal signaling for mediating IL-1R-TLR induced NFkappaB activation
Pacurari et al., Environ Health Perspect 2008 (Neoplasms, Mesothelial) : In the present study, we exposed mesothelial cells to SWCNTs and investigated reactive oxygen species ( ROS ) generation, cell viability, DNA damage, histone H2AX phosphorylation, activation of poly(ADP-ribose) polymerase 1 ( PARP-1 ), stimulation of extracellular signal regulated kinase ( ERKs ), Jun N-terminal kinases (JNKs), protein p38, and activation of activator protein-1 (AP-1), nuclear factor kappaB (NF-kappaB) , and protein serine-threonine kinase ( Akt ) ... In the present study, we exposed mesothelial cells to SWCNTs and investigated reactive oxygen species ( ROS ) generation, cell viability, DNA damage, histone H2AX phosphorylation, activation of poly(ADP-ribose) polymerase 1 ( PARP-1 ), stimulation of extracellular signal regulated kinase ( ERKs ), Jun N-terminal kinases (JNKs), protein p38, and activation of activator protein-1 (AP-1), nuclear factor kappaB (NF-kappaB) , and protein serine-threonine kinase ( Akt ) ... In the present study, we exposed mesothelial cells to SWCNTs and investigated reactive oxygen species ( ROS ) generation, cell viability, DNA damage, histone H2AX phosphorylation, activation of poly(ADP-ribose) polymerase 1 ( PARP-1 ), stimulation of extracellular signal regulated kinase ( ERKs ), Jun N-terminal kinases (JNKs) , protein p38, and activation of activator protein-1 (AP-1), nuclear factor kappaB (NF-kappaB) , and protein serine-threonine kinase ( Akt )
Xu et al., Cancer Chemother Pharmacol 2009 (Neoplasms) : Based on the preclinical evidence of topoisomerase I ( Topo-1 ) upregulation by mitomycin C ( MMC ) and decreased NF-kappaB activation by celecoxib, we evaluated combinations of irinotecan/MMC and irinotecan/MMC/celecoxib in patients with advanced solid malignancies
Bai et al., Am J Nephrol 2009 (Kidney Failure, Chronic...) : Voluntary exercise reversed NF-kappaB activation and lowered NAD ( P ) H oxidase , PAI-1, MCP-1 and COX-2 abundance, increased LV mass by raising myofibrillar proteins and ameliorated anemia without affecting renal function or arterial pressure
Okamura et al., American journal of physiology. Renal physiology 2008 : Subsequent experiments revealed that acute ablation of GRP78 protein by AB5 subtilase cytotoxin caused reinforcement of MCP-1 induction and NF-kappaB activation by TNF-alpha and that transfection with GRP78 significantly suppressed the cytokine induced activation of NF-kappaB
Kim et al., Mol Cells 2008 : In addition, rosmarinic acid reduced cell migration by inducing the expression of a specific chemokine receptor on LPS induced mature BMDCs. Rosmarinic acid also significantly reduced the expressions of MCP-1 and MIP-lalpha induced by LPS in BMDCs and inhibited LPS induced activation of MAPK and the nuclear translocation of NF-kappaB ... In addition, rosmarinic acid reduced cell migration by inducing the expression of a specific chemokine receptor on LPS induced mature BMDCs. Rosmarinic acid also significantly reduced the expressions of MCP-1 and MIP-lalpha induced by LPS in BMDCs and inhibited LPS induced activation of MAPK and the nuclear translocation of NF-kappaB ... In addition, rosmarinic acid reduced cell migration by inducing the expression of a specific chemokine receptor on LPS induced mature BMDCs. Rosmarinic acid also significantly reduced the expressions of MCP-1 and MIP-lalpha induced by LPS in BMDCs and inhibited LPS induced activation of MAPK and the nuclear translocation of NF-kappaB
Boost et al., BMC cancer 2008 (Lung Neoplasms...) : These observations concurred with lack of a modulatory activity of IFNgamma on IL-1beta induced NF-kappaB activation as assessed by cellular IkappaB levels
Mamedova et al., Pharmacol Res 2008 (Reperfusion Injury) : The NF-kappaB activation was attenuated by 10nM MRS2693 , which activated the antiapoptic ERK1/2 pathway
Zhang et al., Inflammation 2008 (Inflammation) : As a precursor of GSH, glutamine could prevent the NF-kappaB activation and attenuate the release of TNF-alpha in LPS stimulated AT-II cells and the effect may be mediated via GSH synthesis
Eswarappa et al., Innate Immun 2008 : Role of actin cytoskeleton in LPS induced NF-kappaB activation and nitric oxide production in murine macrophages ... Here, we have investigated the role of actin cytoskeleton in LPS induced NF-kappaB activation and signaling leading to the expression of iNOS and nitric oxide production ... Using murine macrophages, we show that disruption of actin cytoskeleton by either cytochalasin D ( CytD ) or latrunculin B ( LanB ) does not affect LPS induced NF-kappaB activation and the expression of iNOS, a NF-kappaB target gene
Harrison et al., Infect Immun 2008 : Vibrio cholerae flagellins induce Toll-like receptor 5-mediated interleukin-8 production through mitogen activated protein kinase and NF-kappaB activation ... Each purified recombinant V. cholerae flagellin induced IL-8 production in T84 intestinal epithelial cells and also induced nuclear factor kappa B (NF-kappaB) activation in HEK293T/TLR5 transfectants, which was blocked by cotransfection with a TLR5 dominant negative construct, demonstrating TLR5 specificity
El-Osta et al., J Exp Med 2008 (Diabetes Complications...) : Both the epigenetic changes and the gene expression changes persist for at least 6 d of subsequent normal glycemia, as do NF-kappaB induced increases in monocyte chemoattractant protein 1 and vascular cell adhesion molecule 1 expression ... Both the epigenetic changes and the gene expression changes persist for at least 6 d of subsequent normal glycemia, as do NF-kappaB induced increases in monocyte chemoattractant protein 1 and vascular cell adhesion molecule 1 expression
Liu et al., Respir Res 2008 : CSE stimulated NF-kappaB -DNA binding activity and up-regulated Bcl-XL protein in HBECs ... The current study demonstrates that CSE activates NF-kappaB and up-regulates Bcl-XL through NF-kB activation in HBECs, and that CSE induces cell death in cells lacking p65
Kim et al., J Virol 2008 : An intronic NF-kappaB binding site within the Bcl3 gene served as the primary target of Tax induced NF-kappaB activation
Eckmann et al., Proc Natl Acad Sci U S A 2008 (Acute Disease...) : Here, we show in murine models that inhibition of IKKbeta dependent NF-kappaB activation exacerbates acute inflammation, but attenuates chronic inflammatory disease in the intestinal tract
Ye et al., Nan Fang Yi Ke Da Xue Xue Bao 2008 : To investigate the effect of rosiglitazone on the expression of nuclear factor-kappaB (NF-kappaB) and coupling factor 6 ( CF6 ) induced by tumor necrosis factor-alpha (TNF-alpha) in cultured human umbilical vein endothelial cells ( HUVEC )
Hiratsuka et al., Nat Cell Biol 2008 (Neoplasm Metastasis...) : In our study, SAA3 stimulated NF-kappaB signalling in a TLR4 dependent manner and facilitated metastasis ... In our study, SAA3 stimulated NF-kappaB signalling in a TLR4 dependent manner and facilitated metastasis ... In our study, SAA3 stimulated NF-kappaB signalling in a TLR4 dependent manner and facilitated metastasis
Ishinaga et al., Biochem J 2009 : TGF-beta1 synergistically enhances TNF-alpha induced NF-kappaB DNA binding activity via induction of RelA acetylation ... TGF-beta1 synergistically enhances TNF-alpha induced NF-kappaB DNA binding activity via induction of RelA acetylation
Hong et al., Vascul Pharmacol 2009 : The present study reports that TLR-4-agonistic LPS upregulates IL-8 at the transcriptional and post-translational level in VSMCs, and that ERK1/2, NF-kappaB , and C/EBP play major roles in the upregulation of IL-8
Kang et al., Gastroenterology 2008 (Stomach Neoplasms) : NF-kappaB activates transcription of the RNA binding factor HuR , via PI3K-AKT signaling, to promote gastric tumorigenesis
Van Gammeren et al., FASEB J 2009 (Muscular Atrophy) : Overexpression of a c.a. IKKalpha-EGFP fusion protein showed that IKKalpha was sufficient to activate NF-kappaB activity and induce fiber atrophy in muscle
Inagaki et al., J Biol Chem 2008 : Tumor necrosis factor- and interleukin-1 induced activation of TAK1 was entirely normal in Tab1-deficient MEFs and could activate both mitogen activated protein kinases and NF-kappaB ... Tumor necrosis factor- and interleukin-1 induced activation of TAK1 was entirely normal in Tab1-deficient MEFs and could activate both mitogen activated protein kinases and NF-kappaB
Kallifatidis et al., Gut 2009 (Pancreatic Neoplasms) : Tumour necrosis factor related apoptosis inducing ligand ( TRAIL ) is considered to be a novel anticancer agent ; however, recent studies have shown that many pancreatic cancer cells are resistant to apoptosis induction by TRAIL due to TRAIL activated nuclear factor-kappaB (NF-kappaB) signalling
Takeda et al., Int Immunol 2008 : CD28 stimulation triggers NF-kappaB activation through the CARMA1-PKCtheta-Grb2/Gads axis ... However, the signal transducing cascades linking the CD28 molecule and activation of NF-kappaB remain unclear ... To examine the role of these molecules in NF-kappaB activation through CD28 mediated stimulation, Bcl10 was over expressed in Jurkat cells and their NF-kappaB activation by CD28- or TCR-cross linking was evaluated ... We found that CD28 stimulation alone can induce NF-kappaB activation in Bcl10-over expressing Jurkat cells, whereas TCR stimulation alone has only little effect ... In addition, we found that Bcl10 induced NF-kappaB activation through CD28 mediated stimulation could be blocked by the dominant negative form of PKC or CARMA1 ... In addition, we found that Bcl10 induced NF-kappaB activation through CD28 mediated stimulation could be blocked by the dominant negative form of PKC or CARMA1 ... In addition, we found that Bcl10 induced NF-kappaB activation through CD28 mediated stimulation could be blocked by the dominant negative form of PKC or CARMA1 ... Furthermore, genetic studies revealed that Grb2/Gads binding, but not phosphatidylinositol 3-kinase binding, is important in CD28 mediated NF-kappaB activation
Lee et al., Korean J Parasitol 2008 (Inflammation) : T. gondii lysate triggered nuclear factor (NF)-kappaB dependent IL-8 expression in HEK293 cells transfected with TLR2 ... It is suggested that immunosuppression induced by T. gondii lysate treatment might occur via TLR2 mediated NF-kappaB activation
Cismasiu et al., Biochem J 2009 : BCL11B enhances TCR/CD28 triggered NF-kappaB activation through up-regulation of Cot kinase gene expression in T-lymphocytes ... Enhanced NF-kappaB activation is not a consequence of BCL11B binding to the NF-kappaB response elements or association with the NF-kappaB-DNA complexes, but rather the result of higher translocation of NF-kappaB to the nucleus caused by enhanced degradation of IkappaB ( inhibitor of NF-kappaB ) ... The implied involvement of Cot kinase in BCL11B mediated NF-kappaB activation in response to TCR activation is supported by the fact that a Cot kinase dominant negative mutant or Cot kinase siRNA ( small interfering RNA ) knockdown blocked BCL11B mediated NF-kappaB activation ... The implied involvement of Cot kinase in BCL11B mediated NF-kappaB activation in response to TCR activation is supported by the fact that a Cot kinase dominant negative mutant or Cot kinase siRNA ( small interfering RNA ) knockdown blocked BCL11B mediated NF-kappaB activation ... In support of our observations, in the present study we report that BCL11B enhances the expression of several other NF-kappaB target genes, in addition to IL-2
Lee et al., J Immunol 2008 (Inflammation...) : Cutting edge : Inhibition of NF-kappaB mediated TSLP expression by retinoid X receptor ... We have shown that the human TSLP gene is regulated in a NF-kappaB dependent manner ; however the factors that negatively regulate TSLP expression are not known
Zhan et al., J Immunol 2008 : Glucocorticoid induced TNF receptor expression by T cells is reciprocally regulated by NF-kappaB and NFAT
Jennewein et al., J Immunol 2008 : We provide evidence that peroxisome proliferator activated receptor gamma ( PPARgamma ) coordinates antiinflammatory responses following its activation by AC. Exposing murine RAW264.7 macrophages to AC before LPS stimulation reduced NF-kappaB transactivation and lowered target gene expression of, that is, TNF-alpha and IL-6 compared with controls ... In macrophages overexpressing a dominant negative mutant of PPARgamma, NF-kappaB transactivation in response to LPS was restored, while macrophages from myeloid lineage-specific conditional PPARgamma knockout mice proved that PPARgamma transmitted an antiinflammatory response, which was delivered by AC. Expressing a PPARgamma-Delta aa32-250 deletion mutant, we observed no inhibition of NF-kappaB
Kim et al., Biochem Biophys Res Commun 2008 (Astrocytoma) : In addition, TRAIL induces the DNA binding activity of NF-kappaB , an important transcription factor for MMP-9 induction ... The specific MEK inhibitor, U0126, significantly blocks TRAIL mediated NF-kappaB activation and subsequent MMP-9 induction ... The specific MEK inhibitor, U0126, significantly blocks TRAIL mediated NF-kappaB activation and subsequent MMP-9 induction
Brach et al., Pathobiology 1991 : The results suggest that M-CSF induction of M-CSF involves G proteins, PKC and NF kappa B
Ghosh et al., Chem Biol Interact 2009 (Disease Models, Animal...) : Investigating the cell signaling pathways, we found that the protein exerts its protective action via the activation of NF-kappaB and Akt and deactivation of STAT-1
Kamiyama et al., Am J Physiol Lung Cell Mol Physiol 2008 (Pneumonia...) : Nevertheless, methylprednisolone treatment strongly eliminated LPS induction of NF-kappaB activity, as determined by NF-kappaB nuclear translocation and by gel mobility shift assays
Slevogt et al., Nat Immunol 2008 : Here we demonstrate that the interaction of CEACAM1 with ubiquitous surface protein A1 expressed on M. catarrhalis or with opacity associated proteins on N. meningitidis resulted in reduced Toll-like receptor 2-initiated transcription factor NF-kappaB dependent inflammatory responses of primary pulmonary epithelial cells ... Here we demonstrate that the interaction of CEACAM1 with ubiquitous surface protein A1 expressed on M. catarrhalis or with opacity associated proteins on N. meningitidis resulted in reduced Toll-like receptor 2-initiated transcription factor NF-kappaB dependent inflammatory responses of primary pulmonary epithelial cells
Oh et al., Eur J Pharmacol 2008 (Inflammation) : Withaferin A inhibits iNOS expression and nitric oxide production by Akt inactivation and down regulating LPS induced activity of NF-kappaB in RAW 264.7 cells ... Moreover, LPS induced NO production and NF-kappaB activation were inhibited by SH-6, a specific inhibitor of Akt
Carbone et al., Mol Pharmacol 2009 : The data presented here demonstrate the efficacy of a novel anti-inflammatory diindolylmethane class compound, 1,1-bis ( 3'-indolyl ) -1- ( p-t-butylphenyl ) methane ( DIM-C-pPhtBu ), in suppressing NF-kappaB dependent expression of inducible nitric-oxide synthase ( NOS2 ) and NO production in astrocytes exposed to the parkinsonian neurotoxicant 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine ( MPTP ) through a mechanism distinct from that described for the thiazolidinedione-class compound, rosiglitazone
Yun et al., J Agric Food Chem 2008 (Edema) : Sinapic acid suppressed the LPS induced activation of nuclear factor-kappaB (NF-kappaB) , a transcription factor pivotal necessary for pro-inflammatory mediators, such as iNOS, COX-2, TNF-alpha, and IL-1beta
Zhang et al., Zhonghua Xue Ye Xue Za Zhi 2008 (Multiple Myeloma) : The activity of NF-kappaB was enhanced by BDNF in a dose dependent manner, and pretreatment with K252 alpha could significantly inhibit this activation at 1 , 6, 12 and 24 h ( P < 0.05 ) in a time dependent manner ... The activity of NF-kappaB was enhanced by BDNF in a dose dependent manner, and pretreatment with K252 alpha could significantly inhibit this activation at 1, 6, 12 and 24 h ( P < 0.05 ) in a time dependent manner ... BDNF plays an important role in the angiogenesis of MM to promote the up-regulation of MMP-9 , which may be induced by enhanced NF-kappaB activity in MM cells
Dong et al., Phytother Res 2009 (Brain Ischemia...) : In vitro, the translocation of NF-kappaB was inhibited by TSA and the survival rate of astrocytes was markedly increased and the NO production was decreased
Fukano et al., Cytokine 2008 (Fever...) : Time lag between the increase of IL-6 with fever and NF-kappaB activation in the peripheral blood in inflammatory myofibroblastic tumor
Yuan et al., Am J Physiol Gastrointest Liver Physiol 2008 (Pancreatitis) : Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells ... Protein kinase D1 mediates NF-kappaB activation induced by cholecystokinin and cholinergic signaling in pancreatic acinar cells ... Previous studies in our laboratory demonstrated that protein kinase C ( PKC ) isoforms PKCdelta and epsilon are key regulators of NF-kappaB activation induced by cholecystokinin-8 (CCK-8), tumor necrosis factor-alpha , and ethanol ... Previous studies in our laboratory demonstrated that protein kinase C ( PKC ) isoforms PKCdelta and epsilon are key regulators of NF-kappaB activation induced by cholecystokinin-8 (CCK-8) , tumor necrosis factor-alpha, and ethanol ... Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol ( CCh ), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh ... Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol ( CCh ), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh ... Here, we demonstrate that protein kinase D1 (PKD1) is a key downstream target of PKCdelta and PKCepsilon in pancreatic acinar cells stimulated by two major secretagogues, CCK-8 and the cholinergic agonist carbachol ( CCh ), and that PKD1 is necessary for NF-kappaB activation induced by CCK-8 and CCh ... Either inhibition of PKCdelta or epsilon by isoform-specific inhibitory peptides, genetic deletion of PKCdelta and epsilon in pancreatic acinar cells, or knockdown of PKD1 by using small interfering RNAs in AR42J cells resulted in a marked decrease in PKD1 and NF-kappaB activation stimulated by CCK-8 or CCh. Conversely, overexpression of PKD1 resulted in augmentation of CCK-8- and CCh stimulated NF-kappaB activation
Shen et al., J Am Coll Nutr 2008 (Atherosclerosis...) : PPAR can inhibit NF-kappaB signaling, and our previous data have shown that PPAR transactivation activity requires adequate zinc
Zhao et al., Cell Death Differ 2009 : hnRNP A1 regulates UV-induced NF-kappaB signalling through destabilization of cIAP1 mRNA
Kundu et al., Cancer Lett 2009 (Skin Neoplasms) : Taken together, the above findings suggest that oligonol inhibits TPA induced COX-2 expression by blocking the activation of NF-kappaB and C/EBP via modulation of MAP kinases and suppresses chemically induced mouse skin tumorigenesis
Sanada et al., J Biol Chem 2008 : Forced expression of FLN29 inhibited the IPS-1 dependent activation of both NF-kappaB and IRF3 ... Forced expression of FLN29 inhibited the IPS-1 dependent activation of both NF-kappaB and IRF3
Chen et al., J Biol Chem 2008 : Here we report that the transcription of the GlcNAc6ST-1 gene, the gene encoding a sulfotransferase for 6-sulfated glycan synthesis, is induced in human T-lymphoid cells through tandem NF-kappaB and GATA motifs in its 5'-regulatory region
Hanson et al., J Allergy Clin Immunol 2008 (Immunologic Deficiency Syndromes) : Although both L153R and C417R impaired TLR and TNF-alpha induced NF-kappaB activation, L153R also increased TNF-alpha induced programmed cell death with decreased A20 expression
Kunnumakkara et al., Mol Cancer Ther 2008 (Neoplasm Invasiveness...) : This labdane also suppressed constitutive NF-kappaB activity in different cell lines and inhibited IkappaBalpha kinase activation, thus leading to the suppression of IkappaBalpha phosphorylation, degradation, p65 nuclear translocation, and reporter gene transcription
Sarkar et al., Int Rev Immunol 2008 (Cell Transformation, Neoplastic...) : More importantly, recent studies have shown that NF-kappaB signaling also plays critical roles in the epithelial-mesenchymal transition (EMT) and cancer stem cells
Wu et al., J Biol Chem 2008 : In rat growth plate chondrocytes, IGF-I induced NF-kappaB-p65 nuclear translocation ... Moreover, the inhibition of the phosphatidylinositol 3-kinase and Akt abolished the effects of IGF-I on NF-kappaB activation
Cho et al., Coron Artery Dis 2008 (Coronary Artery Disease) : The objective of the present study was to investigate the expression of TGase 2 in the human atherosclerotic human coronary artery, and the possible roles of TGase 2 in NF-kappaB activation
Voegeli et al., Appl Physiol Nutr Metab 2008 : The role of Hsp27 and Hsp70 in NF-kappaB activation is reviewed here, along with evidence suggesting that HSPs regulate Ang II-induced blood pressure through the regulation of NF-kappaB
Zhang et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2008 (Disease Models, Animal) : Compared with control group, SOD activity in LPS group decreased at different time points significantly ( P < 0.05 or P < 0.01 ), but MPO activity, MDA content and the expression of NF-kappaB increased obviously ( P < 0.05 or P < 0.01 ) ; the administration of MT and DEX could mitigate above values significantly ( P < 0.05 or P < 0.01 )
Matsuda et al., Blood 2009 : Nevertheless, both PI3K and Btk are required for the activation of NF-kappaB , a critical transcription factor family for B-cell development and function ... Nevertheless, both PI3K and Btk are required for the activation of NF-kappaB , a critical transcription factor family for B-cell development and function
Zhu et al., Transplant Proc 2008 : We undertook this study to determine whether CD154-CD40 interactions induced human endothelial cell ( EC ) activation via the nuclear factor (NF)-kappaB pathway, and whether the upregulation of monocyte derived CD40 and CD80 is NF-kappaB pathway dependent ... We undertook this study to determine whether CD154-CD40 interactions induced human endothelial cell ( EC ) activation via the nuclear factor (NF)-kappaB pathway, and whether the upregulation of monocyte derived CD40 and CD80 is NF-kappaB pathway dependent ... We undertook this study to determine whether CD154-CD40 interactions induced human endothelial cell ( EC ) activation via the nuclear factor (NF)-kappaB pathway, and whether the upregulation of monocyte derived CD40 and CD80 is NF-kappaB pathway dependent ... The NF-kappaB inhibitor suppressed upregulation of monocytederived CD40 and CD80 ... The NF-kappaB inhibitor suppressed upregulation of monocytederived CD40 and CD80
Sun et al., Cell Signal 2009 : PPM1A and PPM1B act as IKKbeta phosphatases to terminate TNFalpha induced IKKbeta-NF-kappaB activation ... Overexpression of PPM1A or PPM1B results in dephosphorylation of IKKbeta at Ser177 and Ser181 and termination of IKKbeta induced NF-kappaB activation ... Furthermore, knockdown of PPM1A and PPM1B expression enhances TNFalpha induced IKKbeta phosphorylation, NF-kappaB nuclear translocation and NF-kappaB dependent gene expression ... These data suggest that PPM1A and PPM1B play an important role in the termination of TNFalpha mediated NF-kappaB activation through dephosphorylating and inactivating IKKbeta
Cheng et al., Chin J Physiol 2008 : In this study, we found that menthone can suppress the lipopolysaccharide (LPS) induced proinflammatory cytokines, interleukin-1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), as well as nuclear factor kappaB (NF-kappaB) activity induced by LPS and other inflammatory agents, including 12-O-tetradecanoylphorbol-13-acetate, hydrogen peroxide, okadaic acid, and ceramide
Moreira et al., Invest Ophthalmol Vis Sci 2009 (Choroidal Neovascularization) : Inhibition of NF-kappaB with BAY 11-7082 reduced IL-8 but not VEGF induction ... Inhibition of NF-kappaB with BAY 11-7082 reduced IL-8 but not VEGF induction
Sokol et al., Proc Natl Acad Sci U S A 2008 (Colitis...) : In Caco-2 cells transfected with a reporter gene for NF-kappaB activity, F. prausnitzii had no effect on IL-1beta induced NF-kappaB activity, whereas the supernatant abolished it
Pietkiewicz et al., Postepy Hig Med Dosw 2008 (Atherosclerosis...) : Engagement of RAGE in intracellular signaling leads to the activation of the proinflammatory transcription factor NF-kappaB to sustained cellular dysfunction and tissue destruction
Kang et al., Eur J Pharmacol 2008 : These results suggest that PA isolated from A. cordata has a potential regulatory effect on inflammatory iNOS, COX-2 and IL-6 expression through blockade of the phosphorylation of MAPKs following I kappaB alpha degradation and NF-kappaB activation
Kingeter et al., J Immunol 2008 : Loss of protein kinase C theta, Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset ... Loss of protein kinase C theta , Bcl10, or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset ... Loss of protein kinase C theta, Bcl10 , or Malt1 selectively impairs proliferation and NF-kappa B activation in the CD4+ T cell subset ... CD4 ( + ) T cells from knockout mice are extremely deficient in TCR induced NF-kappaB activation, whereas NF-kappaB activation is only partially impaired in CD8 ( + ) T cells
Kaiser et al., J Immunol 2008 : Suppression of RIP1 expression by RNAi abrogates NF-kappaB activation as well as IFNbeta induction by immunostimulatory DNA
Graham et al., Prostate 2009 (Neoplasm Metastasis...) : PI3K/Akt dependent transcriptional regulation and activation of BMP-2-Smad signaling by NF-kappaB in metastatic prostate cancer cells ... PI3K/Akt dependent transcriptional regulation and activation of BMP-2-Smad signaling by NF-kappaB in metastatic prostate cancer cells ... PI3K/Akt dependent transcriptional regulation and activation of BMP-2-Smad signaling by NF-kappaB in metastatic prostate cancer cells ... PI3K/Akt dependent transcriptional regulation and activation of BMP-2-Smad signaling by NF-kappaB in metastatic prostate cancer cells ... In this study, we investigated whether the nuclear transcription factor NF-kappaB , implicated in PC metastasis, is involved in transcriptional regulation and activation of BMP-2 or BMP-4/Smad signaling in PC cells ... In this study, we investigated whether the nuclear transcription factor NF-kappaB , implicated in PC metastasis, is involved in transcriptional regulation and activation of BMP-2 or BMP-4/Smad signaling in PC cells ... In this study, we investigated whether the nuclear transcription factor NF-kappaB , implicated in PC metastasis, is involved in transcriptional regulation and activation of BMP-2 or BMP-4/Smad signaling in PC cells ... Selective inhibition of PI3K/Akt suppressed the NF-kappaB induced BMP-2 promoter activity ... Notably, the activation of BMPRII by BMP-2 is required for modulation of Smad activation by NF-kappaB in PC cells ... Notably, the activation of BMPRII by BMP-2 is required for modulation of Smad activation by NF-kappaB in PC cells ... Notably, the activation of BMPRII by BMP-2 is required for modulation of Smad activation by NF-kappaB in PC cells ... Based on ChIP analysis, the transcriptional regulation of BMP-2 gene by NF-kappaB may be partially attributed to binding to kappab site on the BMP-2 promoter
Zhang et al., Zhongguo Dang Dai Er Ke Za Zhi 2008 (Bronchopulmonary Dysplasia...) : The present study was aimed to investigate the effects of lipopolysaccharide (LPS) and hyperoxia exposure on the nuclear factor-kappa B (NF-kappaB) expression in human embryo lung fibroblasts ( HELFs ) in vitro ... Both LPS and hyperoxia exposure induced NF-kappaB activation in the HELFs in vitro
Wu et al., Free Radic Biol Med 2009 (Cataract) : The data showed that, unlike transient oxidative stress, sustained exposure of HLEC to physiologically relevant levels of H ( 2 ) O ( 2 ) ( 50-100 microM for 4 h ) did not induce the degradation of I-kappaB and activation of NF-kappaB, but attenuated TNFalpha induced degradation of I-kappaB and activation of NF-kappaB ... Consistent with the role of the proteasome in degradation of I-kappaB and activation of NF-kappaB, treatment of HLEC with proteasome inhibitors also attenuated TNFalpha induced I-kappaB degradation and NF-kappaB activation
Sudo et al., Biochim Biophys Acta 2008 : In conclusion, The TNF-alpha and IL-6 signals from the blood are necessary for liver regeneration and NF-kappaB and STAT3 binding are activated via TNF-alpha and IL-6 signal pathways ... In conclusion, The TNF-alpha and IL-6 signals from the blood are necessary for liver regeneration and NF-kappaB and STAT3 binding are activated via TNF-alpha and IL-6 signal pathways
Yoneda et al., Int J Oncol 2008 (Carcinoma, Hepatocellular...) : Dual topology of functional Toll-like receptor 3 expression in human hepatocellular carcinoma : differential signaling mechanisms of TLR3 induced NF-kappaB activation and apoptosis
Mitsuhashi et al., Int J Oncol 2008 : TNFalpha activated NF-kappaB and associated regulatory factors including IKK are strongly implicated in a variety of hematological and solid tumor malignancies
Li et al., Biochim Biophys Acta 2009 : The zinc finger containing protein A20 is a negative regulator of TNF induced JNK ( c-Jun-N-terminal kinase ) and NFkappaB ( nuclear factor kappaB ) signaling
Moon et al., Cancer Lett 2009 (Neoplasms) : Sulforaphane suppresses TNF-alpha mediated activation of NF-kappaB and induces apoptosis through activation of reactive oxygen species dependent caspase-3 ... Sulforaphane suppresses TNF-alpha mediated activation of NF-kappaB and induces apoptosis through activation of reactive oxygen species dependent caspase-3 ... We also report that SFN non-specifically inhibited TNF-alpha induced NF-kappaB activation through the inhibition of IkappaBalpha phosphorylation, IkappaBalpha degradation, and p65 nuclear translocation ... We also report that SFN non-specifically inhibited TNF-alpha induced NF-kappaB activation through the inhibition of IkappaBalpha phosphorylation, IkappaBalpha degradation, and p65 nuclear translocation ... These effects suggest that SFN inhibits TNF-alpha induced NF-kappaB activation through the suppression of IkappaBalpha degradation, leading to reduced expression of NF-kappaB regulated gene products ... These effects suggest that SFN inhibits TNF-alpha induced NF-kappaB activation through the suppression of IkappaBalpha degradation, leading to reduced expression of NF-kappaB regulated gene products ... In conclusion, the results of the present study indicate that SFN suppresses TNF-alpha induced NF-kappaB activity and induces apoptosis through activation of ROS dependent caspase-3 ... In conclusion, the results of the present study indicate that SFN suppresses TNF-alpha induced NF-kappaB activity and induces apoptosis through activation of ROS dependent caspase-3 ... In conclusion, the results of the present study indicate that SFN suppresses TNF-alpha induced NF-kappaB activity and induces apoptosis through activation of ROS dependent caspase-3
Sun et al., Proc Natl Acad Sci U S A 2008 : Beta-arrestin 2 is required for lysophosphatidic acid induced NF-kappaB activation ... Although LPA induced signaling pathways have been intensively investigated, the molecular mechanism by which LPA activates NF-kappaB is not fully defined ... In this work, we found that beta-arrestin 2, but not beta-arrestin 1, is required for LPA induced NF-kappaB activation and interlukin-6 expression
Di Paola et al., Intensive Care Med 2009 (Hypertension...) : Administration of glycyrrhizin, significantly reduced the ( a ) fall of mean arterial blood pressure, ( b ) mortality rate, ( c ) myeloperoxidase (MPO) activity, ( d ) production of pro-inflammatory cytokines [tumor necrosis factor-alpha ( TNF-alpha ) and interleukin-1beta (IL-1beta) ], ( e ) histological evidence of gut injury, ( f ) immunoreactivity of nitrotyrosine, ( g ) poly ADP-ribose ( PAR ) formation, ( h ) the expression of ICAM-1 and P-selectin, ( i ) activation of nuclear factor-kappaB (NF-kappaB) and ( j ) signal transducer and activator transcription-3 ( STAT-3 ) induced by splanchnic artery occlusion-reperfusion shock ... Administration of glycyrrhizin, significantly reduced the ( a ) fall of mean arterial blood pressure, ( b ) mortality rate, ( c ) myeloperoxidase (MPO) activity, ( d ) production of pro-inflammatory cytokines [tumor necrosis factor-alpha ( TNF-alpha ) and interleukin-1beta (IL-1beta) ], ( e ) histological evidence of gut injury, ( f ) immunoreactivity of nitrotyrosine, ( g ) poly ADP-ribose ( PAR ) formation, ( h ) the expression of ICAM-1 and P-selectin, ( i ) activation of nuclear factor-kappaB (NF-kappaB) and ( j ) signal transducer and activator transcription-3 ( STAT-3 ) induced by splanchnic artery occlusion-reperfusion shock
Ghosh-Choudhury et al., Breast Cancer Res Treat 2009 (Adenocarcinoma...) : NFkappaB DNA binding activity and NFkappaB dependent transcription of Bcl-2 and Bcl-XL genes were also prevented by DHA and EPA treatment ... NFkappaB DNA binding activity and NFkappaB dependent transcription of Bcl-2 and Bcl-XL genes were also prevented by DHA and EPA treatment ... Finally, we showed that PTEN expression significantly inhibited NFkappaB dependent transcription of Bcl-2 and Bcl-XL genes ... Finally, we showed that PTEN expression significantly inhibited NFkappaB dependent transcription of Bcl-2 and Bcl-XL genes
Anand et al., Mol Immunol 2009 (MAP Kinase Signaling System...) : Inhibition of Pyk2 activity using a pharmacological inhibitor, Tyrphostin A9 significantly attenuated LPS induced Pyk2 tyrosine phosphorylation, p38 MAP kinase ( MAPK ) activation, NF-kappaB activation, and MCP-1 expression ... Inhibition of Pyk2 activity using a pharmacological inhibitor, Tyrphostin A9 significantly attenuated LPS induced Pyk2 tyrosine phosphorylation, p38 MAP kinase ( MAPK ) activation, NF-kappaB activation, and MCP-1 expression
Iwasaki et al., Mol Cell Endocrinol 2009 (Inflammation) : We examined the effect of insulin on the transcriptional activity of NF-kappaB , which mediates the expression of a variety of inflammation/coagulation related genes using hepatocyte cell lines in vitro
Warshakoon et al., Antimicrob Agents Chemother 2009 : We observed that these compounds do bind to and neutralize LTA, as reflected by the inhibition of TLR2 mediated NF-kappaB induction in reporter gene assays
Pecaric-Petkovic et al., Blood 2009 (Hypersensitivity) : These effects are similar to that of IL-3, but the signaling pathways engaged are distinct because IL-33 strongly activates NF-kappaB and shows a preference for p38 MAP-kinase, while IL-3 acts through Jak/Stat and preferentially activates ERK
Lee et al., J Microbiol Biotechnol 2008 : Lactobacillus casei 3260 ( L. casei 3260 ) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS) induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells ... Therefore, these findings suggest that the suppression of COX-2 through inhibiting the NF-kappaB activation by LPS may be associated with the antiinflammatory effects of L. casei 3260 on Raw264.7 cells
Higashimoto et al., J Biol Chem 2008 : Expressing a constitutively active Plk1 in mammalian cells reduced tumor necrosis factor (TNF) induced IKK activation, resulting in decreased phosphorylation of endogenous IkappaBalpha and reduced NF-kappaB activation
Pruett et al., J Immunotoxicol 2006 : The suppression of IL-6 production and inhibition of NF-kappa B activation was greater than additive when comparing animals given both compounds to those given either compound separately
Kim et al., BMB Rep 2008 : Furthermore, increased DNA binding activity of NF-kappaB in response to TNF-alpha , an agonist of NF-kappaB signaling, was seen in differentiated but not undifferentiated mouse ES cells
Luo et al., Mol Immunol 2009 (Acute Lung Injury...) : We have reported that intracellular glutathione S-transferases P1 (GSTP1) suppresses LPS ( lipopolysaccharide ) -induced excessive production of pro-inflammatory factors by inhibiting LPS stimulated MAPKs ( mitogen activated protein kinases ) as well as NF-kappaB activation
Tsai et al., J Virol 2009 : K15M localizes to lysosomal membranes and induces cell migration, invasion, and NF-kappaB ( but not AP-1 ) activity via its conserved SH2 binding motif
Wang et al., Hepatology 2008 (Hepatoblastoma...) : On the other hand, NF-kappaB activation suppressed FXR mediated gene expression both in vitro and in vivo, indicating a negative crosstalk between the FXR and NF-kappaB signaling pathways
Zhi et al., J Pathol 2009 (Carcinoma...) : These effects were accompanied by a reduction in COX-2 expression and in activity of NF-kappaB , a known regulator of COX-2 ... Taken together, these findings suggest that SmgGDS promotes the development and progression of prostate cancer, possibly associated with NF-kappaB dependent up-regulation of COX-2
Anand et al., Cancer Res 2008 : Electrophoretic mobility shift assay showed that pretreatment with picroliv abrogated tumor necrosis factor (TNF) induced activation of NF-kappaB
Kim et al., Mol Cancer Res 2008 (Glioma) : Here, we report that the Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin ( 17-AAG ) blocks MMP-9 secretion and that HA-induced nuclear factor-kappaB (NF-kappaB) activation is mediated by IkappaB kinase, which phosphorylates the NF-kappaB inhibitor IkappaBalpha and promotes its degradation ... Here, we report that the Hsp90 inhibitor 17-allylamino-17-demethoxygeldanamycin ( 17-AAG ) blocks MMP-9 secretion and that HA-induced nuclear factor-kappaB (NF-kappaB) activation is mediated by IkappaB kinase , which phosphorylates the NF-kappaB inhibitor IkappaBalpha and promotes its degradation ... Importantly, we show that 17-AAG acts by blocking focal adhesion kinase activation, thereby inhibiting IkappaB kinase dependent IkappaBalpha phosphorylation/degradation, NF-kappaB activation, and MMP-9 expression ... Based on our data, we propose that 17-AAG is a candidate drug for treatment of highly invasive gliomas resulting from HA-induced, NF-kappaB mediated MMP-9 secretion
He et al., Arthritis Rheum 2008 (Arthritis, Experimental...) : Inhibition of ROK by specific inhibitors or ROK small interfering RNA suppressed lipopolysaccharide- or TNFalpha induced NF-kappaB nuclear translocation, DNA binding activity, luciferase reporter gene expression, and IkappaBalpha degradation
Jin et al., J Pain 2009 (Neuralgia) : Variants of neural nitric oxide synthase in the spinal cord of neuropathic rats and their effects on nuclear factor-kappaB (NF-kappaB) activity in PC12 cells
Wang et al., Cancer Cell 2008 (Rhabdomyosarcoma) : Results demonstrate that in myoblasts, miR-29 is repressed by NF-kappaB acting through YY1 and the Polycomb group
Blackwell et al., Mol Cell Biol 2009 (Lymphoma) : Notably, TRAF2 phosphorylation increased both basal and inducible c-Jun and NF-kappaB activities and rendered cells resistant to stress induced apoptosis ... Notably, TRAF2 phosphorylation increased both basal and inducible c-Jun and NF-kappaB activities and rendered cells resistant to stress induced apoptosis
Irazoqui et al., Proc Natl Acad Sci U S A 2008 : Overexpression of human homologs of egl-5 modulated NF-kappaB dependent TLR2 signaling in epithelial cells
Yoshida et al., J Biol Chem 2008 (MAP Kinase Signaling System) : These data suggest that IPS-1 requires TRAF6 and MEKK1 to activate NF-kappaB and mitogen activated protein kinases that are critical for the optimal induction of type I interferons ... These data suggest that IPS-1 requires TRAF6 and MEKK1 to activate NF-kappaB and mitogen activated protein kinases that are critical for the optimal induction of type I interferons ... These data suggest that IPS-1 requires TRAF6 and MEKK1 to activate NF-kappaB and mitogen activated protein kinases that are critical for the optimal induction of type I interferons
Lee et al., J Mol Med (Berl) 2009 : These results suggest that p38 MAPK may lead to the activation of IKK and NF-kappaB signaling, resulting in enhanced DC maturation and CXCL2 expression in response to C. difficile toxin A stimulation
Zhang et al., Clin Exp Pharmacol Physiol 2009 (Adrenal Gland Neoplasms...) : The inhibition of NF-kappaB by TPCK enhanced caspase 3 activity induced by 300 micromol/L H(2)O ( 2 )
Eberlein et al., Journal of inflammation (London, England) 2008 : Mechanistically, NAC and DMSO inhibit HA induced gene expression by inhibiting NF-kappaB activation, but NAC had no influence on HA-fragment-AP-1 mediated gene expression
Thurau et al., J Virol 2009 : The induction of MnSOD expression was dependent on vFLIP/K13 mediated activation of NF-kappaB , occurred in a cell-intrinsic manner, and was correlated with decreased intracellular superoxide accumulation and increased resistance of endothelial cells to superoxide induced death
Zhang et al., Arterioscler Thromb Vasc Biol 2009 (Diabetic Angiopathies...) : Adenovirus mediated overexpression of AdipoR1 and 2 in ECs significantly enhanced the suppressive effect of a subeffective dose of adiponectin on TNF-alpha induced ICAM-1 expression and NF-kappaB activation ... Adenovirus mediated overexpression of AdipoR1 and 2 in ECs significantly enhanced the suppressive effect of a subeffective dose of adiponectin on TNF-alpha induced ICAM-1 expression and NF-kappaB activation
Pauli et al., PLoS Pathog 2008 : Influenza A virus inhibits type I IFN signaling via NF-kappaB dependent induction of SOCS-3 expression
Mustonen et al., Dig Dis Sci 2009 : Taurocholate potentiates ethanol induced NF-kappaB activation and inhibits caspase-3 activity in cultured rat gastric mucosal cells
Bair et al., J Biol Chem 2009 (Thrombosis) : Ca2+ signaling is involved in NF-kappaB activation, but the role of store operated Ca2+ entry is unclear ... Expression of a PKCdelta-T505A mutant suppressed the thrombin induced but not the TNF-alpha induced NF-kappaB activation
Feng et al., Immunol Lett 2008 (Hepatitis, Animal) : Furthermore, TET inhibited NF-kappaB activity, the critical transcriptional factor of the above mentioned inflammatory cytokines, by preventing the activation of IkappaBalpha kinasealpha (IKKalpha) and then inhibiting phosphorylation of IkappaBalpha to stabilize IkappaBalpha in intrahepatic leukocytes ... Furthermore, TET inhibited NF-kappaB activity, the critical transcriptional factor of the above mentioned inflammatory cytokines, by preventing the activation of IkappaBalpha kinasealpha (IKKalpha) and then inhibiting phosphorylation of IkappaBalpha to stabilize IkappaBalpha in intrahepatic leukocytes
Rascle et al., Exp Cell Res 2009 : The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes ... Inhibition of NF-kappaB activity by short interfering RNA mediated knock-down of p65 impairs, while activation of NF-kappaB activity by TNF-alpha synergizes induction of NF-kappaB target genes by LMX1B
Oh et al., Apoptosis 2008 (Leukemia) : Furthermore, we have shown that overexpression of Bcl-2 and active Akt ( myr-Akt ) in U937 cells inhibited the induction of apoptosis, activation of caspase-3, and PLC-gamma1 cleavage by withaferin A. Taken together, our results indicated that the JNK and Akt pathways and inhibition of NF-kappaB activity were key regulators of apoptosis in response to withaferin A in human leukemia U937 cells
Vallabhapurapu et al., Eur J Immunol 2008 : NF-kappaB also regulates TCR induced expression of LT-alpha and LT-beta within NKT cells ... NF-kappaB also regulates TCR induced expression of LT-alpha and LT-beta within NKT cells
Lee et al., Biochem Biophys Res Commun 2009 (Arthritis, Rheumatoid) : Infection of FLS with Ad-IkappaB alpha ( S32A, S36A ), an adenovirus containing mutant IkappaB alpha, inhibited IL-1beta induced nuclear translocation and DNA binding of NF-kappaB
Devaraj et al., J Leukoc Biol 2009 : These effects appear to be mediated via activation of NF-kappaB via CD32 and CD64 ... These effects appear to be mediated via activation of NF-kappaB via CD32 and CD64
Sorriento et al., Proc Natl Acad Sci U S A 2008 : The G-protein coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha ... The G-protein coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha ... To evaluate the effect of GRK5-IkappaB alpha interaction on NFkappaB signaling, we induced the overexpression and the knockdown of GRK5 in cell cultures ... To evaluate the effect of GRK5-IkappaB alpha interaction on NFkappaB signaling, we induced the overexpression and the knockdown of GRK5 in cell cultures ... Our results reveal an unexpected role for GRK5 in the regulation of NFkappaB transcription activity
Fraunberger et al., Shock 2009 (Disease Models, Animal...) : We investigated the effect of simvastatin on LPS induced nuclear factor kappaB (NF-kappaB) activation , TNF release, and mortality in guinea pigs, an animal model with a lipoprotein profile and pattern similar to humans
Du et al., Diabetes Res Clin Pract 2009 (Diabetes Mellitus, Type 1...) : Activation of NF-kappaB and amounts of IL-1beta and TNF-alpha production by stimulation with ligands significantly increased in LADA and T2DM, which was modulated by 1,25 ( OH ) ( 2 ) D3 to similar level, as compared to controls
Zhang et al., Cancer Res 2008 (Pancreatic Neoplasms) : This study reveals IKI-1 as a potent small molecule inhibitor of IKKalpha and IKKbeta, which effectively blocked TNFalpha mediated IKK activation and subsequent NF-kappaB activity
Regueiro et al., Eur Respir J 2009 : LBP and sCD14 inhibited the nontypeable Haemophilus influenzae ( NTHi ) -dependent secretion of IL-8 and the activation of NF-kappaB and p38 mitogen activated protein kinase signalling pathways but they increased the internalisation of NTHi by airway epithelial cells ... LBP and sCD14 inhibited the nontypeable Haemophilus influenzae ( NTHi ) -dependent secretion of IL-8 and the activation of NF-kappaB and p38 mitogen activated protein kinase signalling pathways but they increased the internalisation of NTHi by airway epithelial cells
Yadav et al., Invest Ophthalmol Vis Sci 2009 (Cataract) : Cell growth was assessed by 3- ( 4,5-dimethylthiazol-2-yl ) -2,5-diphenyl tetrazolium bromide ( MTT ) assay and cell counting, the expression of alpha-SMA , beta-crystallin, and ICAM-1 by Western blot and immunocytochemical analysis, protein kinases by Western blot analysis, and NF-kappaB activation by gel shift and reporter assays ... Cell growth was assessed by 3- ( 4,5-dimethylthiazol-2-yl ) -2,5-diphenyl tetrazolium bromide ( MTT ) assay and cell counting, the expression of alpha-SMA, beta-crystallin, and ICAM-1 by Western blot and immunocytochemical analysis, protein kinases by Western blot analysis, and NF-kappaB activation by gel shift and reporter assays
Sumariwalla et al., Rheumatology (Oxford) 2009 (Arthritis, Experimental...) : Inhibition of TNF-alpha in monocytes was mediated partially through a nuclear factor-kappaB (NF-kappaB) dependent pathway, as judged by sustained levels of IkappaBalpha in cytosolic extracts and a reduced level of LPS induced NF-kappaB activity in nuclear extracts
Gutierrez et al., Cell Microbiol 2009 : In uninfected cells, sphingomyelin ( SM ), phosphatidylinositol-4-phosphate (PIP) and arachidonic acid ( AA ) enhanced NF-kappaB activation and the cell surface expression of CD69, a macrophage activation marker regulated by NF-kappaB
Chastre et al., FASEB J 2009 (Colorectal Neoplasms) : The TRIP6Stop473 mutant, which lacks the PDZ binding motif, was still able to increase NF-kappaB and Akt activities but did not promote invasiveness or interfere with cell-cell aggregation
Song et al., Proc Natl Acad Sci U S A 2008 : Association with active LMP1 signaling complexes was also critical for IRF7 activation because (i) a dominant negative IRF7 bound to LMP1, blocked IRF7 association and activation, but did not inhibit LMP1 induced NF-kappaB or TBK1 or Sendai virus mediated IFN stimulated response element activation ; and ( ii ) two different LMP1 transmembrane domain mutants, which fail to aggregate, each bound IRF7 and prevented LMP1 from binding and activating IRF7 in the same cell, but did not prevent NF-kappaB activation ... Association with active LMP1 signaling complexes was also critical for IRF7 activation because (i) a dominant negative IRF7 bound to LMP1, blocked IRF7 association and activation, but did not inhibit LMP1 induced NF-kappaB or TBK1 or Sendai virus mediated IFN stimulated response element activation ; and ( ii ) two different LMP1 transmembrane domain mutants, which fail to aggregate, each bound IRF7 and prevented LMP1 from binding and activating IRF7 in the same cell, but did not prevent NF-kappaB activation ... Association with active LMP1 signaling complexes was also critical for IRF7 activation because (i) a dominant negative IRF7 bound to LMP1, blocked IRF7 association and activation, but did not inhibit LMP1 induced NF-kappaB or TBK1 or Sendai virus mediated IFN stimulated response element activation ; and ( ii ) two different LMP1 transmembrane domain mutants, which fail to aggregate, each bound IRF7 and prevented LMP1 from binding and activating IRF7 in the same cell, but did not prevent NF-kappaB activation
Sanchez-Lockhart et al., J Immunol 2008 : CD28 mediated signaling through PI3K results in the recruitment of protein kinase C-theta (PKCtheta) to the cSMAC, activation of NF-kappaB , and up-regulation of IL-2 transcription ... CD28 mediated signaling through PI3K results in the recruitment of protein kinase C-theta (PKCtheta) to the cSMAC, activation of NF-kappaB , and up-regulation of IL-2 transcription
Prêle et al., J Immunol 2008 : Analysis of the early signaling pathway downstream of TLR4 demonstrated that SOCS1 had no regulatory effect on the activation or on the DNA binding capacity of NFkappaB
Kidd et al., Neurogastroenterol Motil 2009 (Crohn Disease) : We examined whether interleukin (IL)1beta and Escherichia coli lipopolysaccharide (LPS) induced EC cell 5HT release through Toll-like/IL-1 (TIL) receptor activation, nuclear factor kappa B (NFkappaB) and mitogen activated protein kinase ( MAPK ) phosphorylation and evaluated whether somatostatin could inhibit this phenomenon ... We examined whether interleukin (IL)1beta and Escherichia coli lipopolysaccharide (LPS) induced EC cell 5HT release through Toll-like/IL-1 (TIL) receptor activation, nuclear factor kappa B (NFkappaB) and mitogen activated protein kinase ( MAPK ) phosphorylation and evaluated whether somatostatin could inhibit this phenomenon ... IL1beta caused significant ( P < 0.05 ) NFkappaB and MAPK phosphorylation ( 40-55 % )
Ma et al., J Biol Chem 2009 (Acquired Immunodeficiency Syndrome) : HIV-1 Nef inhibits lipopolysaccharide induced IL-12p40 expression by inhibiting JNK activated NFkappaB in human monocytic cells ... HIV-1 Nef inhibits lipopolysaccharide induced IL-12p40 expression by inhibiting JNK activated NFkappaB in human monocytic cells ... We have previously shown that lipopolysaccharide (LPS) induced IL-12p40 production in monocytic cells is regulated by NFkappaB and AP-1 transcription factors through the activation of two distinct upstream signaling pathways, namely the c-Jun-N-terminal kinase (JNK) and the calmodulin dependent protein kinase-II activated pathways ... We have previously shown that lipopolysaccharide (LPS) induced IL-12p40 production in monocytic cells is regulated by NFkappaB and AP-1 transcription factors through the activation of two distinct upstream signaling pathways, namely the c-Jun-N-terminal kinase (JNK) and the calmodulin dependent protein kinase-II activated pathways
Taylor et al., J Virol 2009 : We report here that the nucleocapsid ( N ) protein of HTNV was able to inhibit TNF-alpha induced activation of NF-kappaB , as measured by a reporter assay, and the activation of endogenous p65, an NF-kappaB subunit
Baek et al., Oncol Rep 2008 (Carcinoma...) : Suppression of the EGF induced uPAR promoter activity by the AP-1 decoy oligonuclotide, as well as expression vectors encoding mutated-type NF-kappaB inducting kinase and I-kappaB, confirmed that the activation of AP-1 and NF-kappaB are essential for the EGF induced uPAR upregulation
Meng et al., Toxicol Lett 2009 : Mechanistic studies indicate that B [ a ] P-induced transcriptional activation of MMP-3 is not mediated by AP-1 , NF-kappaB ... Mechanistic studies indicate that B [ a ] P-induced transcriptional activation of MMP-3 is not mediated by AP-1, NF-kappaB
Szołtysek et al., Acta biochimica Polonica 2008 : TNFalpha induced activation of NFkappaB protects against UV-induced apoptosis specifically in p53-proficient cells
Sawaya et al., J Neuroimmune Pharmacol 2009 (HIV Infections) : This study examines the role of NF-kappaB in the regulation of TNFalpha production by morphine in microglia ... The use of a small interfering RNA directed against p65, a subunit of NF-kappaB, demonstrated that TNFalpha induction by morphine is NF-kappaB dependent
Andou et al., Gastroenterology 2009 (Chronic Disease...) : Histidine inhibited LPS induced NF-kappaB in macrophages
Foka et al., Int J Biochem Cell Biol 2009 : Inhibition of NF-kappaB using pharmacological agents or by transfection of a plasmid specifying for a superrepressor attenuated the TNF-alpha inhibited C/EBPalpha promoter activity
Ringseis et al., Regul Pept 2009 : Treatment with 50 microM of Ser-Ser-Ser and Glu-Glu-Glu, but not Val-Pro-Leu caused an inhibition of TNFalpha induced activation of NF-kappaB compared to control cells ( P < 0.05 )
Wang et al., Zhonghua Jie He He Hu Xi Za Zhi 2008 (Acute Lung Injury) : PPAR-gamma agonist ( troglitazone ) decreased the expression levels of inflammatory mediators such as TNF-alpha, reduced infiltration and activation of inflammatory cells in lung tissues, and alleviated LPS induced through PPAR-gamma upregulation and inhibition of NF-kappaB activity
Fineschi et al., Arthritis Rheum 2008 (Fibrosis...) : AFA binding to fibroblasts resulted in concomitant activation of ERK-1/2, c-Jun, and NF-kappaB
Roumeguère et al., Prog Urol 2008 (Cardiovascular Diseases) : ApoA-1 production, which is recognized as a cardioprotective lipid fraction, is down regulated by NFkappaB activation in vitro
Minakawa et al., Biol Pharm Bull 2008 : In this study, we examined the effect of ISGylation on tumor necrosis factor receptor associated factor ( TRAF) 6/transforming growth factor beta activated kinase ( TAK)-1 dependent NF-kappaB activation ... In this study, we examined the effect of ISGylation on tumor necrosis factor receptor associated factor ( TRAF) 6/transforming growth factor beta activated kinase ( TAK)-1 dependent NF-kappaB activation
Yang et al., Biol Pharm Bull 2008 : Moreover, TNF-alpha production was prevented by NF-kappaB and mitogen activated protein kinase ( MAPK ) inhibitors ... Activation of NF-kappaB and ERK1/2 were necessary for CD80 , CD86 and MHCII expression induced by semi-vioxanthin ... Activation of NF-kappaB and ERK1/2 were necessary for CD80, CD86 and MHCII expression induced by semi-vioxanthin
Dey et al., Nat Rev Drug Discov 2008 (Neoplasms) : Although drugs are being designed to selectively activate p53 or inhibit NF-kappaB , there is no concerted effort yet to deliberately make drugs that can simultaneously do both
Shin et al., PLoS Pathog 2008 : This enhanced response involved NF-kappaB activation by TLR signaling as well as Nod1 and Nod2 detection of type IV secretion
Fukazawa et al., PLoS Pathog 2008 (Dysentery, Bacillary) : GEF-H1 mediated control of NOD1 dependent NF-kappaB activation by Shigella effectors ... GEF-H1 mediated control of NOD1 dependent NF-kappaB activation by Shigella effectors ... GEF-H1 interacts with NOD1 and is required for RIP2 dependent NF-kappaB activation by H-Ala-D-gammaGlu-DAP ( gammaTriDAP ) ... GEF-H1 interacts with NOD1 and is required for RIP2 dependent NF-kappaB activation by H-Ala-D-gammaGlu-DAP ( gammaTriDAP ) ... GEF-H1 interacts with NOD1 and is required for RIP2 dependent NF-kappaB activation by H-Ala-D-gammaGlu-DAP ( gammaTriDAP ) ... GEF-H1 interacts with NOD1 and is required for RIP2 dependent NF-kappaB activation by H-Ala-D-gammaGlu-DAP ( gammaTriDAP ) ... GEF-H1 is essential for NF-kappaB activation by the Shigella effectors IpgB2 and OspB, which were found to signal in a NOD1 and RhoA Kinase ( ROCK ) dependent manner
Fan et al., J Biol Chem 2009 (MAP Kinase Signaling System...) : Src enhanced basal and SF stimulated NF-kappaB activity and SF protection against ADR, in a manner dependent upon its kinase and Src homology 3 domains ; and endogenous Src was required for SF stimulation of NF-kappaB activity and cell protection ... The ability of Src to enhance SF stimulation of NF-kappaB activity was due, in part, to its ability to stimulate Akt and IkappaB kinase activity ; and Src mediated stimulation of NF-kappaB was due , in part, to a Rac1/MKK3/6/p38 pathway and was Akt dependent ... The ability of Src to enhance SF stimulation of NF-kappaB activity was due, in part, to its ability to stimulate Akt and IkappaB kinase activity ; and Src mediated stimulation of NF-kappaB was due , in part, to a Rac1/MKK3/6/p38 pathway and was Akt dependent ... The ability of Src to enhance SF stimulation of NF-kappaB activity was due, in part, to its ability to stimulate Akt and IkappaB kinase activity ; and Src mediated stimulation of NF-kappaB was due, in part, to a Rac1/MKK3/6/p38 pathway and was Akt dependent ... The NF-kappaB inducing kinase was found to act downstream of TAK1 ( transforming growth factor-beta activated kinase 1 ) as a mediator of SF- and Src stimulated NF-kappaB activity
Bourguignon et al., J Biol Chem 2009 (Breast Neoplasms) : Further analyses indicate that activation of SIRT1 ( deacetylase ) by resveratrol ( a natural antioxidant ) induces SIRT1-p300 association and acetyltransferase inactivation, leading to deacetylation of HA/CD44 induced beta-catenin and NFkappaB-p65 , inhibition of beta-catenin-T-cell factor/lymphocyte enhancer factor and NFkappaB-specific transcriptional activation, and the impairment of MDR1 and Bcl-x ( L ) gene expression
Najat et al., J Bone Miner Res 2009 (Osteitis Deformans) : Finally, effects of the wildtype and mutant SQSTM1 on NF-kappaB signaling were assessed in HEK293 cells co-transfected with an NF-kappaB luciferase reporter construct
Pan et al., J Biol Chem 2009 : Tubocapsanolide A inhibits transforming growth factor-beta activating kinase 1 to suppress NF-kappaB induced CCR7 ... A bioactive withanolide tubocapsanolide A ( Tubo A ) suppressed NF-kappaB mediated CCR7 expression in breast cancer cells and attenuated their migration toward lymphatic endothelial cells ... We conclude that Tubo A inhibits TAK1 to repress NF-kappaB induced CCR7 expression in breast cancer cells and suggest that Tubo A may be useful for the prevention of lymphatic invasion of breast cancer cells
Utreras et al., J Biol Chem 2009 (Pain) : MEK inhibitor, which inhibits ERK activation, decreased p35 promoter activity, whereas the inhibitors of p38 MAPK, JNK, and NF-kappaB increased p35 promoter activity, indicating that these pathways regulate p35 expression differently
Lee et al., Am J Chin Med 2008 : Moreover, the suppression of PPY on the cytokine productions resulted from the inhibition of inhibitory kappaB-alpha phosphorylation and nuclear factor-kappaB (NF-kappaB) activation ... Analysis of the anti-inflammatory effects of ursolic acid and oleanolic acid, the triterpene compounds present in PPY, showed that ursolic acid significantly inhibited LPS induced IL-8 production, NF-kappaB activation , and iNOS mRNA expression, whereas oleanolic acid did not have these effects
Healy et al., J Leukoc Biol 2009 : Suppression of PDLIM2 expression in THP-1 and RAW 264.7 cells resulted in decreased adhesion, increased NF-kappaB transcription reporter activity, and increased LPS induced TNF-alpha production
Papadopoulou et al., Molecular cancer 2008 (Prostatic Neoplasms) : Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells ... Membrane androgen receptor activation triggers down-regulation of PI-3K/Akt/NF-kappaB activity and induces apoptotic responses via Bad, FasL and caspase-3 in DU145 prostate cancer cells
Shant et al., Exp Cell Res 2009 (Colonic Neoplasms) : Akt dependent NF-kappaB activation is required for bile acids to rescue colon cancer cells from stress induced apoptosis ... We hypothesized that bile acid induced EGFR activation also mediates cell survival by downstream Akt regulated activation of NF-kappaB ... We hypothesized that bile acid induced EGFR activation also mediates cell survival by downstream Akt regulated activation of NF-kappaB ... Both DCT induced NF-kappaB nuclear translocation and attenuation of TNF-alpha stimulated apoptosis were dependent on EGFR activation ... Both DCT induced NF-kappaB nuclear translocation and attenuation of TNF-alpha stimulated apoptosis were dependent on EGFR activation ... Likewise, transfection with mutant akt and treatment with a chemical inhibitor of Akt attenuated effects of DCT on NF-kappaB transcriptional activity and TNF-alpha induced apoptosis ... Collectively, these observations indicate that, downstream of EGFR, bile acid induced colon cancer cell survival is mediated by Akt dependent NF-kappaB activation
Liu et al., Regul Pept 2009 : Furthermore, we observed that NAC also inhibited UVB induced activation of NF-kappaB/p65 through decreasing the degradation and phosphorylation of IkappaBalpha
Noh et al., Rheumatology (Oxford) 2009 (Arthritis, Rheumatoid...) : Moreover, cordycepin significantly inhibited IL-1beta induced p38/JNK and AP-1 activation, but not extracellular signal regulated kinase ( ERK ) and NF-kappaB activation
Novak et al., Nucleic Acids Res 1991 : Our results suggest that maximal transcriptional activation of the uPA gene by PMA, IL-1 and TNF alpha requires the induction of NFkB activity and the decay of a short lived repressor protein, possibly IkB ... Our results suggest that maximal transcriptional activation of the uPA gene by PMA, IL-1 and TNF alpha requires the induction of NFkB activity and the decay of a short lived repressor protein, possibly IkB ... Our results suggest that maximal transcriptional activation of the uPA gene by PMA, IL-1 and TNF alpha requires the induction of NFkB activity and the decay of a short lived repressor protein, possibly IkB
Ren et al., Acta Pharmacol Sin 2009 : Further studies showed that IGF-1 specifically induced NF-kappaB activity
Marrero et al., Brain Res 2009 (Inflammation) : Convergence of alpha 7 nicotinic acetylcholine receptor activated pathways for anti-apoptosis and anti-inflammation : central role for JAK2 activation of STAT3 and NF-kappaB ... In this study, we investigated the effects of inhibiting the alpha7 nAChR-JAK2 pro-survival cascade on the nicotine induced production of the survival factor Bcl-2 and the transcriptional activation of NF-kappaB , AP-1, STAT1, STAT3, and STAT5
Wu et al., Free Radic Biol Med 2009 (MAP Kinase Signaling System) : NF-kappaB DNA binding activity was increased by TNFalpha alone but lowered by TNFalpha plus PY ... NF-kappaB activation products such as Bcl-2, Bcl-X ( L ), cFLIP ( S ), cFLIP ( L ), and Mn-SOD were reduced by TNFalpha plus PY and restored by 1400W or NAC
Zhao et al., Blood 2009 : The activation of NF-kappaB by Tax has been reported to play a crucial role in HTLV-1 induced transformation ... In the present study, we observed that HBZ specifically suppressed NF-kappaB-driven transcription mediated by p65 ( the classical pathway ) without inhibiting the alternative NF-kappaB signaling pathway ... In the present study, we observed that HBZ specifically suppressed NF-kappaB-driven transcription mediated by p65 ( the classical pathway ) without inhibiting the alternative NF-kappaB signaling pathway ... Finally, HBZ actually repressed the transcription of some classical NF-kappaB target genes, such as IL-8, IL2RA, IRF4, VCAM-1, and VEGF ... Selective suppression of the classical NF-kappaB pathway by HBZ renders the alternative NF-kappaB pathway predominant after activation of NF-kappaB by Tax or other stimuli, which might be critical for oncogenesis
Pekarsky et al., Proc Natl Acad Sci U S A 2008 (Leukemia, Lymphocytic, Chronic, B-Cell) : To examine the mechanisms by which Tcl1 protein exerts oncogenic activity in B cells, we investigated the effect of Tcl1 expression on NF-kappaB and activator protein 1 (AP-1) activity ... Additionally, Tcl1 activates NF-kappaB by physically interacting with p300/CREB binding protein ... The results indicate that Tcl1 overexpression causes B-CLL by directly enhancing NF-kappaB activity and inhibiting AP-1
Wätjen et al., Mol Nutr Food Res 2009 (Carcinoma, Hepatocellular...) : Furthermore, enniatins A1 and B1, but not enniatin B were able to inhibit moderately tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation
Ammann et al., Int J Cancer 2009 (Neuroblastoma) : By comparison, inhibition of TRAIL stimulated NF-kappaB activation by IkappaBalpha-superrepressor or the small molecule NF-kappaB inhibitor BMS-345541 significantly enhances TRAIL induced apoptosis, pointing to an antiapoptotic function of NF-kappaB in TRAIL mediated apoptosis ... Analysis of signaling pathways reveals that NF-kappaB inhibition prevents TRAIL triggered up-regulation of Mcl-1 , promoting TRAIL induced cytochrome c release and activation of caspases
Turner et al., Mol Ther 2009 : Transcriptional silencing of HIV-1 correlates with an increase in silent-state epigenetic marks including histone and DNA methylation, a loss of nuclear factor-kappaB (NF-kappaB) recruitment, and requires Argonaute 1 (Ago-1) , histone deacetylase 1 (HDAC-1), and DNA methyltransferase 3a (DNMT3a) localization to the LTR ... Transcriptional silencing of HIV-1 correlates with an increase in silent-state epigenetic marks including histone and DNA methylation, a loss of nuclear factor-kappaB (NF-kappaB) recruitment, and requires Argonaute 1 (Ago-1), histone deacetylase 1 (HDAC-1) , and DNA methyltransferase 3a (DNMT3a) localization to the LTR
Lee et al., Inflammation 2009 (Colitis) : The TNBS treatment caused colon shortening, increased myeloperoxidase activity, induced IL-1beta, TNF-alpha , and IL-6 expression in the colon, activated NF-kappaB , and potentiated the GAG degrading activities of intestinal microflora ... The TNBS treatment caused colon shortening, increased myeloperoxidase activity, induced IL-1beta , TNF-alpha, and IL-6 expression in the colon, activated NF-kappaB , and potentiated the GAG degrading activities of intestinal microflora ... The TNBS treatment caused colon shortening, increased myeloperoxidase activity, induced IL-1beta, TNF-alpha, and IL-6 expression in the colon, activated NF-kappaB , and potentiated the GAG degrading activities of intestinal microflora
Park et al., Vascul Pharmacol 2009 : In HT1080 cancer cells, FPP-3 significantly suppressed release of VEGF as well as activation of NF-kappaB , a transcriptional factor required for VEGF expression
Vinciguerra et al., Hepatology 2009 : The promoter activity of microRNA-21 was increased by mTOR/NF-kappaB activation ... CONCLUSION : Unsaturated fatty acids inhibit PTEN expression in hepatocytes by up-regulating microRNA-21 synthesis via an mTOR/NF-kappaB dependent mechanism
Shelton et al., J Biol Chem 2009 (Diabetes Complications...) : This up-regulation of Grx1 was concomitant with NFkappaB activation and induction of intercellular adhesion molecule-1 ( ICAM-1 )
Wang et al., J Biol Chem 2009 (Carcinoma, Hepatocellular) : In the presence of melittin, TRAIL induced apoptosis is significantly increased in TRAIL-resistant HCC cells, which may be attributed to melittin induced TAK1-JNK/p38 activation and melittin mediated inhibition of IkappaBalpha kinase-NFkappaB ... In the presence of melittin, TRAIL induced apoptosis is significantly increased in TRAIL-resistant HCC cells, which may be attributed to melittin induced TAK1-JNK/p38 activation and melittin mediated inhibition of IkappaBalpha kinase-NFkappaB ... Melittin, a major component of bee venom, sensitizes human hepatocellular carcinoma cells to tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) -induced apoptosis by activating CaMKII-TAK1-JNK/p38 and inhibiting IkappaBalpha kinase-NFkappaB ... Melittin, a major component of bee venom, sensitizes human hepatocellular carcinoma cells to tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) -induced apoptosis by activating CaMKII-TAK1-JNK/p38 and inhibiting IkappaBalpha kinase-NFkappaB
Mihm et al., AIDS 1991 : Cysteine and NAC also inhibit NF-kappa B activity as determined by electrophoretic mobility shift assays and chloramphenicol acetyl-transferase (CAT) gene expression under control of NF-kappa B binding sites in uninfected cells
Rafiee et al., Am J Physiol Gastrointest Liver Physiol 2009 : Together, these data demonstrate that 1 ) acid is a potent inducer of IL-6 and IL-8 production in HET-1A cells ; 2 ) MAPK and PKC signaling play a key regulatory role in acid mediated IL-6 and IL-8 expression via NF-kappaB activation ; and 3 ) the anti-inflammatory plant compound curcumin inhibits esophageal activation in response to acid ... Together, these data demonstrate that 1 ) acid is a potent inducer of IL-6 and IL-8 production in HET-1A cells ; 2 ) MAPK and PKC signaling play a key regulatory role in acid mediated IL-6 and IL-8 expression via NF-kappaB activation ; and 3 ) the anti-inflammatory plant compound curcumin inhibits esophageal activation in response to acid
Minhajuddin et al., J Biol Chem 2009 : Furthermore, we observed that expression of the constitutively active form of PKC-delta or Akt was sufficient to induce NF-kappaB activation and ICAM-1 expression, and that co-expression of mTOR suppressed these responses
Fahey et al., Mucosal Immunol 2008 : As SLPI is known to inhibit NF-kB expression, these findings suggest that E ( 2 ) inhibition of proinflammatory cytokines may be mediated through SLPI regulation of NF-kB
Shapiro et al., Nutr Res Rev 2005 : The polyphenolic compound curcumin exhibits antioxidant and anti-inflammatory properties, inhibits NF-kappaB and activates PPAR-gamma
Farombi et al., Life Sci 2009 (Drug-Induced Liver Injury) : Furthermore, kolaviron abrogated DMN induced binding activity of NF-kappaB as well as AP-1
Chen et al., Mol Immunol 2009 : Promoter constructs in a luciferase assay, site directed mutagenesis and laser scanning confocal microscopy assay ( LSCM ) revealed that the transcription factor NF-kappaB is primarily responsible for C5b-9 mediated B7-H1 expression
Zhang et al., Exp Toxicol Pathol 2009 (Inflammation) : The results showed that LPS enhanced nuclear factor kappa B (NF-kappaB) activation and induced a loss in mitochondrial integrity as shown by a significant decrease in membrane potential and increase in mitochondrial permeability transition pore opening ... Pretreatment with catalpol ( 10 mg/kg d, i.p. ) for 10d before injection of LPS reversed the memory deficits induced by LPS, protected brain mitochondrial function, and attenuated LPS induced NF-kappaB activation
Zhang et al., Curr Eye Res 2008 (Endophthalmitis) : A significant inhibitory effect of besifloxacin was observed at 1 or 10 microg/ml. Besifloxacin inhibited IkappaB degradation, NFkappaB nuclear translocation, and activation of p38 and JNK MAPKs
Bhattacharyya et al., Inflamm Bowel Dis 2009 : DSS induced increases in phospho-IkappaBalpha, nuclear NFkappaB ( p65 ), and IL-8 secretion in human colonic epithelial cells in tissue culture are attributable to a reactive oxygen species ( ROS ) -induced pathway of inflammation, and do not require TLR4 , MyD88, or Bcl10, which are associated with the innate immune pathway of NFkappaB-IL-8 activation ... DSS induced increases in phospho-IkappaBalpha, nuclear NFkappaB ( p65 ), and IL-8 secretion in human colonic epithelial cells in tissue culture are attributable to a reactive oxygen species ( ROS ) -induced pathway of inflammation, and do not require TLR4, MyD88 , or Bcl10, which are associated with the innate immune pathway of NFkappaB-IL-8 activation ... DSS induced increases in phospho-IkappaBalpha, nuclear NFkappaB ( p65 ), and IL-8 secretion in human colonic epithelial cells in tissue culture are attributable to a reactive oxygen species ( ROS ) -induced pathway of inflammation, and do not require TLR4, MyD88, or Bcl10, which are associated with the innate immune pathway of NFkappaB-IL-8 activation ... DSS induced increases in phospho-IkappaBalpha, nuclear NFkappaB ( p65 ), and IL-8 secretion in human colonic epithelial cells in tissue culture are attributable to a reactive oxygen species ( ROS ) -induced pathway of inflammation, and do not require TLR4, MyD88, or Bcl10 , which are associated with the innate immune pathway of NFkappaB-IL-8 activation ... Data demonstrated that DSS activates IkappaBalpha, NFkappaB , and IL-8 through an ROS-Hsp27-IKKbeta mediated pathway, and not through an innate immune cascade
Choi et al., Scand J Gastroenterol 2008 (Adenocarcinoma...) : Furthermore, LGG attenuated the IL-1beta induced transcriptional activation of the IL-8 gene and the NF-kappaB-responsive gene, and attenuated the IL-1beta induced IkappaBalpha degradation
Tai et al., J Biol Chem 2009 : Furthermore, SGK1 enhanced nuclear factor kappaB (NF-kappaB) activity and up-regulated N-methyl-d-aspartate receptor NR2A and NR2B expression through activation of IKKalpha at Thr-23 and Ser-180, and these two residues play an equally important role in mediating these effects of SGK1 ... Although SGK1 does not phosphorylate IKKbeta, IKKbeta activity is still required for IKK complex activation and for SGK1 phosphorylation and activation of NF-kappaB ... Although SGK1 does not phosphorylate IKKbeta, IKKbeta activity is still required for IKK complex activation and for SGK1 phosphorylation and activation of NF-kappaB ... In addition, SGK1 increased the acetylation of NF-kappaB through phosphorylation of p300 at Ser-1834, and this also leads to NF-kappaB activation and NR2A and NR2B expression
Wang et al., Int J Cancer 2009 (Carcinoma, Hepatocellular...) : HINT1 inhibits beta-catenin/TCF4, USF2 and NFkappaB activity in human hepatoma cells ... HINT1 also inhibited NFkappaB transcription factor reporter activity and inhibited translocation of the endogenous p65 protein to the nucleus of HepG2 cells
Funakoshi-Tago et al., Cytokine 2009 : TRAF6 negatively regulates TNFalpha induced NF-kappaB activation ... TRAF6 negatively regulates TNFalpha induced NF-kappaB activation ... Also, TNFalpha induced DNA binding activity and transcriptional activation of nuclear factor kappaB (NF-kappaB) were also augmented in TRAF6-deficient MEFs ... Moreover, the reintroduction of exogenous TRAF6 into TRAF6-deficient MEFs clearly suppressed TNFalpha induced IKK activation, NF-kappaB activation and subsequent cytokine expression ... Thus, these data suggest that TRAF6 negatively regulates TNFalpha induced NF-kappaB activation through its ubiquitin ligase activity ... Thus, these data suggest that TRAF6 negatively regulates TNFalpha induced NF-kappaB activation through its ubiquitin ligase activity
Strippoli et al., Dis Model Mech 2008 : Retroviral overexpression of a mutant inhibitor of kappaB ( IkappaB ) demonstrated that NF-kappaB activation is required for E-cadherin and cytokeratin downregulation during EMT
Zayed et al., Arthritis Res Ther 2008 (Osteoarthritis...) : Specific inhibitors of the MAPK p38 ( SB 203580 ) and c-jun N-terminal kinase ( JNK ) ( SP600125 ) and of the NF-kappaB ( SN-50 ) and Notch ( DAPT ) signalling pathways suppressed IL-1beta induced upregulation of L-PGDS expression
Dung et al., Food Chem Toxicol 2009 (Edema) : Moreover, reporter gene analysis revealed that the EO-CO significantly blocked LPS induced transcriptional activation of NF-kappaB in RAW 264.7 cells
Yagi et al., Stroke 2009 (Brain Ischemia...) : We demonstrate that edaravone inhibits rtPA induced cerebral hemorrhage in the ischemic brain of rats via the inhibition of MMP-9 expression in vivo, which is substantiated by inhibition of MMP-9 expression and NF-kappaB activation in HBECs
Schmelzer et al., Biofactors 2008 (Inflammation) : To test this hypothesis, we studied the effect of CoQ10 on the NFkappaB1 dependent pro-inflammatory cytokine TNF-alpha
Zou et al., Am J Physiol Heart Circ Physiol 2009 (Disease Models, Animal...) : LPS ( 2 microg/ml ) increased the levels of IkappaB-alpha phosphorylation, TNF-alpha, ICAM-1, and NF-kappaB in primary cultured cardiomyocytes, which was significantly attenuated by glucosamine treatment and overexpression of O-GlcNAc transferase ; both interventions also significantly increased O-GlcNAc levels ... Glucosamine treatment of macrophage cell line RAW 264.7 also increased O-GlcNAc levels and attenuated the LPS induced activation of NF-kappaB
Kang et al., Blood 2009 (Inflammation) : Here, we found that LECs express high amounts of TLR4 in the intracellular region, and that the TLR4 of LECs is the main mediator of nuclear factor-kappaB (NF-kappaB) activation by LPS
Li et al., Acta Pharmacol Sin 2009 (Neoplasms) : TNF-alpha induced NF-kappaB activation was determined using a reporter gene assay ... TA-35 ( 1-20 micromol/L ) suppressed TNF-alpha induced NF-kappaB activation in transfected cells ( HEK293/pNiFty-SEAP ) in a dose- ( 1-20 micromol/L ) and time dependent ( 0-48 h ) manner ... Subsequently, both NF-kappaB p65 and its inhibitor IkappaB gradually accumulated in cytoplasmic extracts in a dose- and time dependent manner, indicating the blockage of NF-kappaB translocation induced by TNF-alpha from the cytoplasm to the nucleus
Maurais et al., Virology 2009 (HIV Infections) : Importantly, we show that wild-type CD40L remains functional since CD40L bearing virions mediate NF-kappaB activation in a CD40 expressing reporter cell line and induce secretion of the chemokine IL-8 by monocyte derived macrophages
Lam et al., Proc Natl Acad Sci U S A 2008 (Lymphoma, Large B-Cell, Diffuse) : Compensatory IKKalpha activation of classical NF-kappaB signaling during IKKbeta inhibition identified by an RNA interference sensitization screen ... In models of physiologic NF-kappaB pathway activation by CARD11 or tumor necrosis factor-alpha , compensatory IKKalpha activity was also observed with IKKbeta inhibition ... In models of physiologic NF-kappaB pathway activation by CARD11 or tumor necrosis factor-alpha, compensatory IKKalpha activity was also observed with IKKbeta inhibition
Zhang et al., J Immunol 2009 : Whereas LPS induced activation of NF-kappaB and Erk1/2 appears to be unaffected, p38 and Jnk were spontaneously activated and hyperinduced in cFLIP-deficient B cells
Shao et al., Inflamm Res 2008 : Platonin inhibits LPS induced NF-kappaB by preventing activation of Akt and IKKbeta in human PBMC ... Platonin inhibits LPS induced NF-kappaB by preventing activation of Akt and IKKbeta in human PBMC ... Platonin inhibits LPS induced NF-kappaB by preventing activation of Akt and IKKbeta in human PBMC
Zhang et al., Inflamm Res 2008 : Furthermore, ivermectin suppressed NF-kB translocation induced by LPS
Bladh et al., Biochim Biophys Acta 2009 (MAP Kinase Signaling System) : However, TPA treatment alone resulted in much stronger MKP-1 expression in both GRwt and GRR488Q containing cells than that of dexamethasone suggesting that the inability of GRR488Q to inhibit TPA induced NF-kappaB activity did not involve a lack of MKP-1 expression
Yubero et al., Biochim Biophys Acta 2009 (Pancreatitis) : Pancreatic glutathione depletion, acinar overexpression of monocyte chemoattractant protein-1 ( MCP-1 ) and cytokine induced neutrophil chemoattractant ( CINC ), and activation of p38MAPK, NF-kappaB and STAT3 were found in both AP models
Barma et al., Biochim Biophys Acta 2009 (Diabetes Mellitus, Type 2...) : Increase of NF-kappaB did not affect the activation of IKK/IkappaB indicating NF-kappaB p65 expression to be a distinct effect of palmitate
Kalari et al., Am J Physiol Lung Cell Mol Physiol 2009 (MAP Kinase Signaling System) : Furthermore, downregulation of native AGK by AGK small interfering RNA decreased intracellular LPA levels ( approximately 2-fold ) and attenuated LPA induced p38 MAPK, JNK, and NF-kappaB activation, tyrosine phosphorylation of EGF-R, and IL-8 secretion ... Furthermore, downregulation of native AGK by AGK small interfering RNA decreased intracellular LPA levels ( approximately 2-fold ) and attenuated LPA induced p38 MAPK, JNK, and NF-kappaB activation, tyrosine phosphorylation of EGF-R, and IL-8 secretion
Martin et al., J Biol Chem 2009 : Furthermore, we identified the components of the CBM complex, Carma3, Bcl10, and Malt1, as key mediators of the CXCL8/IL8 induced NFkappaB activation and VEGF up-regulation ... Furthermore, we identified the components of the CBM complex, Carma3, Bcl10, and Malt1, as key mediators of the CXCL8/IL8 induced NFkappaB activation and VEGF up-regulation
Walsh et al., PloS one 2008 (MAP Kinase Signaling System) : Further, this NEMO modification contributes to TRAF6 mediated activation of NFkappaB
Li et al., Antioxid Redox Signal 2009 : Endosomal Nox2 facilitates redox dependent induction of NF-kappaB by TNF-alpha ... Cellular stimulation by TNF-alpha induced NADPH dependent superoxide production in the endosomal compartment, and this ROS was required for IKK mediated activation of NF-kappaB ... Cellular stimulation by TNF-alpha induced NADPH dependent superoxide production in the endosomal compartment, and this ROS was required for IKK mediated activation of NF-kappaB ... Inhibiting endocytosis reduced the ability of TNF-alpha to induce both NADPH dependent endosomal superoxide and NF-kappaB , supporting the notion that redox dependent signaling of the receptor occurs in the endosome
Baregamian et al., Biochem Biophys Res Commun 2009 (Enterocolitis, Necrotizing...) : I/R injury resulted in early induction of PPAR-gamma expression and activation of NF-kappaB in small intestine
Lee et al., Exp Mol Med 2008 : Furthermore, transduced PEP-1-PTEN fusion protein inhibited the activation of NF-kappaB induced by LPS
El-Hage et al., PloS one 2008 : Pretreatment with the NF-kappaB inhibitor parthenolide provided evidence that Tat+/-morphine induced release of MCP-1 , IL-6 and TNF-alpha by astrocytes is NF-kappaB dependent ... Pretreatment with the NF-kappaB inhibitor parthenolide provided evidence that Tat+/-morphine induced release of MCP-1, IL-6 and TNF-alpha by astrocytes is NF-kappaB dependent ... Pretreatment with the NF-kappaB inhibitor parthenolide provided evidence that Tat+/-morphine induced release of MCP-1, IL-6 and TNF-alpha by astrocytes is NF-kappaB dependent ... Taken together, these results demonstrate that : 1 ) exposure to Tat+/-morphine is sufficient to activate NF-kappaB and cytokine production, 2 ) the release of MCP-1 and IL-6 by Tat+/-morphine are highly Ca ( 2+ ) -dependent, while TNF-alpha appears to be less affected by the changes in [ Ca ( 2+ ) ] ( i ), and 3 ) in the presence of Tat , exposure to opiates augments Tat induced NF-kappaB activation and cytokine release through a Ca ( 2+ ) -dependent pathway
Bidère et al., Nature 2009 (Lymphoma, Large B-Cell, Diffuse) : ABC DLBCL cells required CK1alpha for constitutive NF-kappaB activity, indicating that CK1alpha functions as a conditionally essential malignancy gene-a member of a new class of potential cancer therapeutic targets
Zhang et al., Clin Sci (Lond) 2009 (Vascular Diseases) : AGEs ( advanced glycation end-products ) /RAGE ( receptor for AGEs ), LOX-1 [ lectin-like oxidized low-density lipoprotein receptor-1 ) and NF-kappaB ( nuclear factor kappaB ) signalling play key roles in TNF-alpha expression through an increase in circulating and/or local vascular TNF-alpha production
Rigaux et al., Allergy 2009 (Hypersensitivity) : IL-12 p40 secretion was dependent on nuclear factor-kappaB (NF-kappaB) , mitogen activated protein ( MAP ) kinases, Toll-like receptor 2 (TLR2), TLR9 and on the bacterial teichoic acid composition ... IL-12 p40 secretion was dependent on nuclear factor-kappaB (NF-kappaB) , mitogen activated protein ( MAP ) kinases, Toll-like receptor 2 (TLR2), TLR9 and on the bacterial teichoic acid composition
Kuroiwa et al., Biochem Biophys Res Commun 2009 : Moreover, inhibition of NF-kappaB signaling suppressed IL-8 mRNA expression induced by bacterial neuraminidase
Ye et al., Biochem Biophys Res Commun 2009 (Inflammation) : ROS and NF-kappaB are involved in upregulation of IL-8 in A549 cells exposed to multi walled carbon nanotubes
Filipovich et al., Am J Obstet Gynecol 2009 (Escherichia coli Infections...) : Intrauterine fetal survival, maintenance of circulating progesterone levels, and nuclear translocation of NFkappaB were also dependent upon MyD88 but not TRIF
Krieg et al., Mol Immunol 2009 : RIP2 promotes NF-kappaB activation as well as activation of the MAPKs JNK, ERK1/2 and p38 MAPK, thereby playing an emergent role in the innate immune response and NOD signaling
Engberding et al., Arterioscler Thromb Vasc Biol 2009 (Diabetes Mellitus, Type 2...) : IGF1R downregulation uncovered an insulin induced reduction in activation of NF-kappaB and inhibition of MCP-1 upregulation in response to TNF-alpha
Zhang et al., Int J Cancer 2009 (Prostatic Neoplasms) : We show that inhibition of NF-kappaB or activation of AP-1 can only partially sensitize resistant prostate cancer cells to proapoptotic effects of TNFalpha or TRAIL ... However, concomitant repression of NF-kappaB and activation of c-Fos/AP-1 significantly enhanced the proapoptotic effects of TNFalpha and TRAIL in resistant prostate cancer cells ... However, concomitant repression of NF-kappaB and activation of c-Fos/AP-1 significantly enhanced the proapoptotic effects of TNFalpha and TRAIL in resistant prostate cancer cells
Li et al., Biochem Biophys Res Commun 2009 (Herpes Genitalis) : More importantly, our data provide direct evidence that the activation of NF-kappaB is required for the production of both IL-6 and IFN-beta induced by HSV-2 in HCE cells ... More importantly, our data provide direct evidence that the activation of NF-kappaB is required for the production of both IL-6 and IFN-beta induced by HSV-2 in HCE cells
Goodridge et al., J Immunol 2009 : A recent report identified a role for CARD9 in innate anti-fungal responses, demonstrating CARD9-Bcl10 mediated activation of NF-kappaB and proinflammatory cytokine induction in murine bone marrow derived DC stimulated via Dectin-1 ... A recent report identified a role for CARD9 in innate anti-fungal responses, demonstrating CARD9-Bcl10 mediated activation of NF-kappaB and proinflammatory cytokine induction in murine bone marrow derived DC stimulated via Dectin-1
Park et al., Carcinogenesis 2009 (Cell Transformation, Neoplastic...) : The upregulation of MMP-2 induced by phosphatidic acid ( PA ), the product of PLD, was mediated by protein kinase C ( PKC ), protein kinase A (PKA), nuclear factor-kappaB (NF-kappaB) and Sp1 and it enhanced glioma cell invasion
Boivin et al., J Interferon Cytokine Res 2009 : IFN-gamma also caused a delayed but more prolonged activation of nuclear factor-kappaB (NF-kappaB) ; inhibition of NF-kappaB also prevented the increase in T84 TJ permeability and alteration in occludin expression ... The IFN-gamma induced activation of NF-kappaB was mediated by a cross-talk with PI3-K pathway
Manna et al., Mol Immunol 2009 (Neoplasms) : P(3)-25 inhibited NF-kappaB DNA binding activity partially, but inhibited NF-kappaB dependent genes expression completely
Huang et al., J Biol Chem 2009 (Cell Transformation, Viral...) : Retroviral Tax proteins from human T cell leukemia virus type 1 and type 2 ( HTLV-1 and -2 ) are capable of activating IKK, yet only HTLV-1 infection causes T cell leukemia, which correlates with persistent activation of NF-kappaB induced by Tax1
Shembade et al., EMBO J 2009 : Furthermore, RNF11 was required for A20 to interact with and inactivate RIP1 to inhibit TNF mediated NF-kappaB activation ... Furthermore, RNF11 was required for A20 to interact with and inactivate RIP1 to inhibit TNF mediated NF-kappaB activation ... Furthermore, RNF11 was required for A20 to interact with and inactivate RIP1 to inhibit TNF mediated NF-kappaB activation
Diebold et al., Thromb Haemost 2008 : In line, RacG12V enhanced HIF-1alpha promoter activity, and this response was dependent on nuclear factor-kappaB (NFkappaB) binding to the HIF-1alpha promoter
Fenske et al., Thromb Haemost 2008 (Inflammation) : Production of endothelial IL-8 and simultaneous modulation of NF-kappaB in response to hydrolyzed LDL might serve to protect the vessel wall and promote silent removal of the insudated lipoprotein
Dragomir et al., Thromb Haemost 2008 : The MAPK inhibitors blocked the phosphorylation of IkBalpha and c-jun, indicating the role of MAPK in NF-kappaB and AP-1 activation in SMC under HG conditions
Schütze et al., Results Probl Cell Differ 2009 (MAP Kinase Signaling System) : The death receptors tumor necrosis factor-receptor 1 ( TNF-R1 ) and CD95 ( Fas, APO-1 ) are known not only to signal for cell death via apoptosis but are also capable of inducing antiapoptotic signals via transcription factor NF-kappaB induction or activation of the proliferative mitogen activated protein kinase ( MAPK ) /ERK ( extracellular signal regulated kinase ) protein kinase cascades, resulting in cell protection and tissue regeneration
Kimura et al., Toxicol Lett 2009 : CYP2E1 expression was required for GCLC promoter-driven gene expression and the activation of NF-kappaB ... Thus ethanol induced GCLC transcription is mediated by not only Nrf2 but also NF-kappaB
Song et al., Int Immunopharmacol 2009 : Activation of the transcriptional activity of NF-kappaB by LPS was also inhibited by treatment with ( - ) -isoeleutherin, suggesting that ( - ) -isoeleutherin mediated suppression of iNOS expression is associated with the regulation of transcription factor NF-kappaB
Shibata et al., J Photochem Photobiol B 2009 (Arthritis, Rheumatoid...) : We also found that IL-1beta induced release of IL-8 from MH7A cells was completely blocked by pretreatment with the ( IL-8 ) inhibitor Bay11-7085, indicating that activation of NF-kappaB signaling plays an important role in the secretion of IL-8
Mi Jeong Sung et al., Int Immunopharmacol 2009 : Vitisin A inhibited LPS induced extracellular signal regulated kinase 1/2 ( ERK1/2 ) and p38 phosphorylation and suppressed LPS induced NF-kappaB activation in RAW 264.7 cells
Gong et al., Bioorg Med Chem Lett 2009 : An IKKbeta inhibitor reported to block NF-kappaB transcriptional activities in Jurkat T cells, was found to enhance NF-kappaB translocation in HUVEC cells
Xu et al., J Biol Chem 2009 (Calcium Signaling) : In addition, PLCgamma2-deficient DCs are also impaired in their activation of NF-kappaB upon Dectin-1 engagement due to defective assembly of the Card9-Bcl10-Malt1 complex and impaired IKKalpha/beta activation and IkappaBalpha degradation
Scazzocchio et al., J Lipid Res 2009 : Moreover, the activation of IKKbeta positively regulated the nuclear content of nuclear factor kappaB (NF-kappaB), by inactivating the inhibitor of NF-kappaB ( IkappaBalpha )
Wu et al., Hepatology 2009 : Pretreatment of hepatocytes and NPCs with HBV-Met cells supernatants, HBsAg, HBeAg, or HBV virions almost completely abrogated TLR induced antiviral activity, which correlated with suppression of interferon beta (IFN-beta) production and subsequent interferon stimulated gene induction as well as suppressed activation of interferon regulatory factor 3 (IRF-3), nuclear factor kappa B (NF-kappaB) , and extracellular signal regulated kinase ( ERK ) 1/2
Pérez-Rodríguez et al., J Neurochem 2009 : IFNgamma and LPS induce nuclear factor kappaB (NFkappaB) translocation to the nucleus, a process implicated in activation of iNOS expression, as inhibition of NFkappaB translocation, by SN50, decreased iNOS expression ... IFNgamma and LPS induce nuclear factor kappaB (NFkappaB) translocation to the nucleus, a process implicated in activation of iNOS expression, as inhibition of NFkappaB translocation, by SN50, decreased iNOS expression ... IFNgamma and LPS induce nuclear factor kappaB (NFkappaB) translocation to the nucleus, a process implicated in activation of iNOS expression, as inhibition of NFkappaB translocation, by SN50, decreased iNOS expression
Xie et al., Biochem Biophys Res Commun 2009 (Breast Neoplasms...) : TLR2 activation enhanced the activity of NF-kappaB and induced phosphorylation of TAK1 and IkappaBalpha in the TLR2/NF-kappaB signaling pathway in MDA-MB-231, but not in MCF-7 cells
Kim et al., J Ethnopharmacol 2009 (Inflammation) : ACL inhibited the expression of IL-8 and IL-6 RNA and protein levels, and attenuated the activation of NF-kappaB and IRF3 after polyI : C treatment ... ACL also inhibited expression of IL-8 and activation of NF-kappaB following PGN induction ... ACL also inhibited expression of IL-8 and activation of NF-kappaB following PGN induction
Choi et al., Cancer Res 2009 (Cell Transformation, Viral) : At a dose of 100 micromol/L, EGCG abrogates p300 induced p65 acetylation in vitro and in vivo, increases the level of cytosolic IkappaBalpha, and suppresses tumor necrosis factor alpha (TNFalpha) induced NF-kappaB activation
Aggarwal et al., Clin Cancer Res 2009 (Anoxia...) : Linkage between cancer and inflammation is indicated by numerous lines of evidence ; first, transcription factors nuclear factor-kappaB (NF-kappaB) and signal transducers and activators of transcription 3 ( STAT3 ), two major pathways for inflammation, are activated by most cancer risk factors ; second, an inflammatory condition precedes most cancers ; third, NF-kappaB and STAT3 are constitutively active in most cancers ; fourth, hypoxia and acidic conditions found in solid tumors activate NF-kappaB ; fifth, chemotherapeutic agents and gamma-irradiation activate NF-kappaB and lead to chemoresistance and radioresistance ; sixth, most gene products linked to inflammation, survival, proliferation, invasion, angiogenesis, and metastasis are regulated by NF-kappaB and STAT3 ; seventh, suppression of NF-kappaB and STAT3 inhibits the proliferation and invasion of tumors ; and eighth, most chemopreventive agents mediate their effects through inhibition of NF-kappaB and STAT3 activation pathways
Yamaguchi et al., Int J Mol Med 2009 : To examine whether estrogen and/or genistein regulate osteoblast differentiation by modulating the NF-kappaB pathway, we examined the effect of 17beta-estradiol and genistein on basal and TNFalpha stimulated NF-kappaB activity in the preosteoblastic cell line MC3T3 ... Our data reveal that while 17beta-estradiol had no effect on basal NF-kappaB activity in MC3T3 cells, it significantly antagonized NF-kappaB activity induced by TNFalpha ( 1 or 10 ng/ml ) ... By contrast, genistein ( 10 ( -6 ) or 10 ( -5 ) M ) significantly increased NF-kappaB activity, and showed no antagonistic effects on TNFalpha induced NF-kappaB promoter activity ... While 17beta-estradiol may stimulate bone anabolism, in part, by antagonizing TNFalpha induced NF-kappaB activation, genistein not only fails to prevent cytokine induced NF-kappaB activation, but directly promotes NF-kappaB activation in MC3T3 cells
Lin et al., Biochem Biophys Res Commun 2009 : In addition, it significantly inhibited RANKL induced NF-kappaB transcriptional activity and nuclear translocation
Mulabagal et al., Phytother Res 2009 : Both S-containing dimeric withanolides, 1 and 2, completely suppressed TNF induced NF-kappaB activation when tested at 100 microm ... In addition, this is the first report on the abrogation of TNF induced NF-kappaB activation for compounds 1 and 2
Gallicchio et al., Br J Pharmacol 2009 : Human PMNs possess functional NK ( 1 ), NK ( 2 ) and NK ( 3 ) receptors, which mediate the induction of COX-2 expression and NF-kappaB activation by SP
Cen et al., J Immunol 2009 : Prior studies demonstrate that SAP enhances TCR induced activation of NF-kappaB in CD4 ( + ) T cells
Hartupee et al., J Immunol 2009 : Although overexpression of TRAF6 in HeLa cells activates NF-kappaB , it does not stabilize transfected KC mRNA
Peloponese et al., J Virol 2009 : Tax activation of NF-kappaB is important for the proliferation and transformation of virus infected cells ... We show here that prolyl isomerase Pin1 is over expressed in HTLV-1 cell lines ; Pin1 binds Tax and regulates Tax induced NF-kappaB activation ... We show here that prolyl isomerase Pin1 is over expressed in HTLV-1 cell lines ; Pin1 binds Tax and regulates Tax induced NF-kappaB activation
Nichols et al., J Virol 2009 : For example, the molluscum contagiosum virus MC160 protein inhibits tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activation ... Since cells expressing either the N or C mutant MC160 protein remained similarly resistant to TNF-alpha induced NF-kappaB activation, the N mutant protein probably utilized a different mechanism for inhibiting NF-kappaB
Bi et al., Zhonghua Yi Xue Za Zhi 2008 (Diabetes Mellitus, Experimental...) : In the DM rats the IkappaBalpha protein expression in the skeletal muscle was significantly lower ( P < 0.05 ) and the NF-kappaB P65 DNA binding activity increased, and TNF-alpha , IL-1B, and IL-6 expression levels were up-regulated in comparison with the normal control group ... In the DM rats the IkappaBalpha protein expression in the skeletal muscle was significantly lower ( P < 0.05 ) and the NF-kappaB P65 DNA binding activity increased, and TNF-alpha, IL-1B , and IL-6 expression levels were up-regulated in comparison with the normal control group ... In the DM rats the IkappaBalpha protein expression in the skeletal muscle was significantly lower ( P < 0.05 ) and the NF-kappaB P65 DNA binding activity increased, and TNF-alpha, IL-1B , and IL-6 expression levels were up-regulated in comparison with the normal control group ... In the DM rats the IkappaBalpha protein expression in the skeletal muscle was significantly lower ( P < 0.05 ) and the NF-kappaB P65 DNA binding activity increased, and TNF-alpha, IL-1B, and IL-6 expression levels were up-regulated in comparison with the normal control group ... In the DM rats the IkappaBalpha protein expression in the skeletal muscle was significantly lower ( P < 0.05 ) and the NF-kappaB P65 DNA binding activity increased , and TNF-alpha, IL-1B, and IL-6 expression levels were up-regulated in comparison with the normal control group
Zhang et al., Cell Signal 2009 (MAP Kinase Signaling System) : On the other hand, prolonged incubation of macrophages with lithium results in dramatic loss of p105 and inhibition of LPS stimulated NF-kappaB activation
Liu et al., Cancer Invest 2009 (Carcinoma, Squamous Cell...) : NS-398 induces apoptosis in human esophageal cancer cells through inhibition of NF-kappaB downstream regulation of cyclooxygenase-2
Bueno et al., Virus Res 2009 (Keratitis, Herpetic) : CDM exerted a potent anti-HSV-1 effect on corneal cells and inhibited NF-kappaB translocation to the nucleus, leading to a decrease in IL-6 production ... However, CDM did not affect NF-kappaB activation in these cells, suggesting that an alternative NF-kappaB cell signaling pathway would be involved in the modulation of cytokine production
Larangé et al., J Leukoc Biol 2009 : We found that upon TLR7 and TLR8 activation, JNK and NF-kappaB positively regulated the expression of CCR7, CD86, CD83, and CD40 and the production of IL-6 and IL-12p40 ... We found that upon TLR7 and TLR8 activation, JNK and NF-kappaB positively regulated the expression of CCR7 , CD86, CD83, and CD40 and the production of IL-6 and IL-12p40 ... We found that upon TLR7 and TLR8 activation, JNK and NF-kappaB positively regulated the expression of CCR7, CD86, CD83 , and CD40 and the production of IL-6 and IL-12p40 ... We found that upon TLR7 and TLR8 activation, JNK and NF-kappaB positively regulated the expression of CCR7, CD86 , CD83, and CD40 and the production of IL-6 and IL-12p40
Venkatachalam et al., J Biol Chem 2009 (Inflammation...) : In isolated adult mouse cardiomyocytes, simulated ischemia/reperfusion enhanced oxidative stress and biologically active IL-18 expression via IKK dependent NF-kappaB activation
Sirabella et al., Stroke 2009 (Calcium Signaling) : Anoxia induced NF-kappaB dependent upregulation of NCX1 contributes to Ca2+ refilling into endoplasmic reticulum in cortical neurons
Cicek et al., Clin Exp Metastasis 2009 : The BRMS1 metastasis suppressor was recently shown to negatively regulate NF-kappaB signaling and down regulate NF-kappaB dependent uPA expression
Musone et al., Nat Genet 2008 (Genetic Predisposition to Disease...) : The TNFAIP3 ( tumor necrosis factor alpha induced protein 3 ) gene encodes a ubiquitin editing enzyme, A20, that restricts NF-kappaB dependent signaling and prevents inflammation
Wang et al., Int Immunopharmacol 2009 (Disease Models, Animal...) : In addition, both HES and BL could attenuate the increase in TNF-alpha, IL-6, MPO levels and NF-kappaB activation
Kizaki et al., Mol Immunol 2009 : Beta2-adrenergic receptor regulate Toll-like receptor 4-induced late-phase NF-kappaB activation ... Stimulation of Toll-like receptor 4 (TLR4) by lipopolysaccharide (LPS) triggers myeloid differentiation factor 88 ( MyD88 ) -dependent early-phase NF-kappaB activation and Toll/IL-1 receptor domain containing adaptor inducing IFN-beta ( TRIF ) -dependent late-phase NF-kappaB activation ... Stimulation of Toll-like receptor 4 (TLR4) by lipopolysaccharide (LPS) triggers myeloid differentiation factor 88 ( MyD88 ) -dependent early-phase NF-kappaB activation and Toll/IL-1 receptor domain containing adaptor inducing IFN-beta ( TRIF ) -dependent late-phase NF-kappaB activation ... On the other hand, depletion of beta ( 2 ) AR or beta-arrestin 2 expression by siRNA decreased cytoplasmic IkappaB alpha and abrogated late-phase IkappaB alpha degradation and NF-kappaB activation in response to LPS
Lee et al., Biochem Biophys Res Commun 2009 (Atherosclerosis) : SAA induced the activations of NF-kappaB and AP-1, which were essential for CCL2 production after SAA stimulation
Lee et al., Toxicology 2009 : Taken together, these results suggest that PKA dependent NF-kappaB inhibition stimulates 15d-PGJ ( 2 ) -mediated mitochondrial apoptotic pathway through alterations in expression of the NF-kappaB target genes Bax
Poljak-Blazi et al., Immunobiology 2009 : TNF-alpha activates NF-kappaB in spleen cells
Furusawa et al., Cell Signal 2009 : Strikingly, Licochalcone A significantly inhibited LPS induced NF-kappaB transcriptional activation ; however, it had no effect on not only the phosphorylation and degradation of IkappaBalpha but also nuclear translocation and DNA binding activity of NF-kappaB p65
Mbalaviele et al., J Pharmacol Exp Ther 2009 (Arthritis...) : Thus, PHA-408 represents a powerful tool to further gain insight into the mechanisms by which IKK-2 regulates NF-kappaB signaling and validates IKK-2 as a therapeutic target
Pang et al., Acta Pharmacol Sin 2009 (Inflammation...) : The LPS induced increase in the DNA binding activity of NF-kappaB and activator protein 1 (AP-1), the nuclear translocation of NF-kappaB p65, the degradation of the inhibitory kappaBalpha protein ( IkappaBalpha ) and the phosphorylation of IkappaBalpha, IkappaB kinase (IKK) alpha/beta, c-Jun NH ( 2 ) -terminal kinase ( JNK ) and p38 MAPKs were all suppressed by FLZ
Vykhovanets et al., Prostate 2009 (Inflammation...) : Concomitant administration of Dex, a known NF-kappaB inhibitor, resulted in significantly down-regulated IL-1 beta induced NF-kappaB/p65 activity, as well as reduced expression of chemokine receptors and IL-17F in mouse prostate tissue
Shchebliakov et al., Mol Gen Mikrobiol Virusol 2008 : [ Mycoplasma M. arginini infection induces constitutive activation of NF-kappaB and inhibits apoptosis in cells expressing toll-like receptors TLR2/6 ]
Yin et al., Zhonghua Zhong Liu Za Zhi 2008 (Liver Neoplasms, Experimental) : H22-CD80/CD86/CD137L ( + ) induces higher NF-kappaB activity of the host T cells by synergistic action of CD28 and CD137, which may be one of the mechanisms of enhancement of the host CTL activity induced by co-expression of CD80, CD86 and CD137L genes ... H22-CD80/CD86/CD137L ( + ) induces higher NF-kappaB activity of the host T cells by synergistic action of CD28 and CD137, which may be one of the mechanisms of enhancement of the host CTL activity induced by co-expression of CD80, CD86 and CD137L genes ... H22-CD80/CD86/CD137L ( + ) induces higher NF-kappaB activity of the host T cells by synergistic action of CD28 and CD137, which may be one of the mechanisms of enhancement of the host CTL activity induced by co-expression of CD80, CD86 and CD137L genes
Cai et al., Cancer Res 2009 (Prostatic Neoplasms) : Phosphorylated GGAP2 can bind the p50 subunit of NF-kappaB and enhances NF-kappaB transcriptional activity
Karwacz et al., J Virol 2009 (Neoplasms) : Furthermore, an enhanced CD8 ( + ) T-cell response, peaking at 7 days, was stimulated by coexpression of p38 MAP kinase or an NF-kappaB activator from the same vector
Takeshima et al., Infect Immun 2009 (Gastritis...) : Inhibitors of NF-kappaB , phosphatidylinositol 3-kinase, p38 mitogen activated protein kinase, and Hsp90 suppressed H. pylori- and VacA induced IL-12 p40 mRNA expression
Graff et al., PLoS Pathog 2009 (Rotavirus Infections) : Rotavirus NSP1 inhibits NFkappaB activation by inducing proteasome dependent degradation of beta-TrCP : a novel mechanism of IFN antagonism ... In this study, we show that rotavirus NSP1 also inhibits activation of NFkappaB and does so by a novel mechanism ... Proteasome mediated degradation of inhibitor of kappaB ( IkappaBalpha ) is required for NFkappaB activation
Méndez-Samperio et al., Cell Immunol 2009 : Finally, the inhibition of PI3K affect NF-kB activation in M. bovis BCG infected cells, indicating that PI3K activity is required for the M. bovis BCG induced activation of NF-kB
Dhingra et al., Cardiovasc Res 2009 : IL-10 attenuates TNF-alpha induced NF kappaB pathway activation and cardiomyocyte apoptosis ... IL-10 attenuates TNF-alpha induced NF kappaB pathway activation and cardiomyocyte apoptosis ... Inhibition of ERK 1/2 MAPK with PD98059 attenuated the protective role of IL-10 against TNF-alpha induced activation of IKK and NF kappaB as well as cardiomyocyte apoptosis ... IL-10 prevents TNF-alpha induced NF kappaB activation and pro-apoptotic changes in cardiomyocytes by inhibiting IKK phosphorylation through the activation of ERK 1/2 MAPK ... IL-10 prevents TNF-alpha induced NF kappaB activation and pro-apoptotic changes in cardiomyocytes by inhibiting IKK phosphorylation through the activation of ERK 1/2 MAPK
Jung et al., Biol Pharm Bull 2009 (Learning Disorders) : Mangiferin also reduced acetylcholine and tumor necrosis factor (TNF)-alpha levels induced by scopolamine in mice brain ( p < 0.05 ) and inhibited nuclear factor (NF)-kappaB activation in scopolamine or TNF-alpha stimulated BV-2 microglial cells
Babu et al., Arch Pharm Res 2009 : Moreover, ACK significantly suppressed TNF-alpha induced translocation of redox-sensitive nuclear factor (NF)-kappaB as well as degradation of cytosolic I-kappaBalpha
Dosch et al., Virus Res 2009 (Severe Acute Respiratory Syndrome) : An NF-kappaB inhibitor, TPCK ( N-Tosyl-L-Phenylalanine Chloromethyl Ketone ) could suppress IL-8 production and secretion in response to S protein in PBMC and THP-1 cells and in HCoV-229E virus infected PBMC
Kortylewski et al., Cancer Cell 2009 (Neoplasms) : Furthermore, Stat3 inhibits NF-kappaB/c-Rel dependent IL-12/p35 gene expression in tumor associated dendritic cells ... Furthermore, Stat3 inhibits NF-kappaB/c-Rel dependent IL-12/p35 gene expression in tumor associated dendritic cells
Fritzenwanger et al., Chin Med J (Engl) 2008 : EMSA experiments show that CT-1 causes nuclear factor (NF) kappaB activation
Bao et al., Mol Pharmacol 2009 : Correspondingly, the elevated HSP27 and HSP70 suppressed inhibitor kappaB degradation and nuclear factor kappaB (NF-kappaB) activation that were caused by ConA
Pediconi et al., Mol Cell Biol 2009 : The NAD ( + ) -dependent histone deacetylase hSirT1 regulates cell survival and stress responses by inhibiting p53-, NF-kappaB- , and E2F1 dependent transcription
De Stefano et al., J Mol Med (Berl) 2009 (Inflammation) : NF-kappaB blockade upregulates Bax , TSP-1, and TSP-2 expression in rat granulation tissue
End et al., Eur J Immunol 2009 : Recent functional and genetic studies linked DMBT1 to the suppression of LPS induced TLR4 mediated NF-kappaB activation and to the pathogenesis of Crohn 's disease ... We propose that DMBT1 functions as pattern-recognition molecule for poly sulfated and poly phosphorylated ligands providing a molecular basis for its broad bacterial binding specificity and its inhibitory effects on LPS induced TLR4 mediated NF-kappaB activation
Sung et al., Cancer Res 2009 (Bone Resorption...) : Zerumbone abolishes RANKL induced NF-kappaB activation, inhibits osteoclastogenesis, and suppresses human breast cancer induced bone loss in athymic nude mice ... We found that zerumbone inhibited RANKL induced NF-kappaB activation in mouse monocyte, an osteoclast precursor cell, through inhibition of activation of IkappaBalpha kinase, IkappaBalpha phosphorylation, and IkappaBalpha degradation ... These results indicate that zerumbone is an effective blocker of RANKL induced NF-kappaB activation and of osteoclastogenesis induced by RANKL and tumor cells, suggesting its potential as a therapeutic agent for osteoporosis and cancer associated bone loss
So et al., Glia 2009 (Cardiovirus Infections) : TMEV infection led to TLR2 mediated NF-kappaB activation , but not IRF3 or IRF7 activation, critical for type I IFN production
Deb et al., American journal of physiology. Renal physiology 2009 (Diabetic Nephropathies...) : In mesangial cells, the stimulation was inhibited by 1,25 ( OH ) ( 2 ) D ( 3 ) ( 20 nM ) or NF-kappaB inhibitor BAY 11-7082, suggesting the involvement of NF- kappaB in HG-induced AGT expression and the interaction between 1,25 ( OH ) ( 2 ) D ( 3 ) and NF-kappaB in the regulation
Senthil Kumar et al., Planta Med 2009 : Lucidone also reduced the translocation of NF-kappaB induced by LPS , which is associated with the prevention of the degradation of I-kappaB, and subsequently decreased p65/p50 protein levels in the nucleus
Kim et al., J Cell Physiol 2009 (Calcium Signaling...) : 5'-N-ethylcarboxamide induces IL-6 expression via MAPKs and NF-kappaB activation through Akt , Ca ( 2+ ) /PKC, cAMP signaling pathways in mouse embryonic stem cells ... NECA induced increase of nuclear factor (NF)-kappaB phosphorylation was inhibited by MAPK inhibitors ... Indeed, NECA induced increase of IL-6 protein expression and secretion was blocked by NF-kappaB inhibitors ... In conclusion, NECA stimulated IL-6 expression via MAPK and NF-kappaB activation through Akt , Ca ( 2+ ) /PKC, and cAMP signaling pathways in mouse ES cells
Li et al., Exp Dermatol 2009 (Candidiasis, Cutaneous) : Anti-TLR2 neutralizing antibody, NFkappaB and p38MAPK inhibitors blocked the PLM induced secretion of IL-6 , IL-8 in keratinocytes, but no such effect was observed in pretreatment with anti-TLR4 neutralizing antibody and lipopolysaccharide inhibitor ( polymyxin B )
Chen et al., J Biol Chem 2009 : IL-1 stimulation of the individual IL-1R1 proteins resulted in the activation of NF-kappaB
Radhakrishnan et al., J Biol Chem 2009 : We found that TcpB could efficiently block TIRAP induced NF-kappaB activation
Schuman et al., Blood 2009 : The kinase TAK1 is essential for T-cell receptor ( TCR ) -mediated nuclear factor kappaB (NF-kappaB) activation and T-cell development ... However, the role of TAK1 in B-cell receptor (BCR) mediated NF-kappaB activation and B-cell development is not clear ... However, the role of TAK1 in B-cell receptor (BCR) mediated NF-kappaB activation and B-cell development is not clear ... Thus, TAK1 is critical for B-cell maturation and BCR induced NF-kappaB activation ... Thus, TAK1 is critical for B-cell maturation and BCR induced NF-kappaB activation
Reber et al., PloS one 2009 (Inflammation) : Transcription of the mast cell growth factor SCF ( stem cell factor ) is upregulated in inflammatory conditions, and this is dependent upon NF-kappaB , as well as the MAP kinases p38 and ERK activation ... We show here that the MAPK downstream nuclear kinase MSK1 induces NF-kappaB p65 Ser276 phosphorylation upon IL-1beta treatment, which was inhibited in cells transfected with a MSK1 kinase-dead ( KD ) mutant compared to the WT control
Kar et al., Eur J Immunol 2009 (Disease Models, Animal...) : Silencing of mitogen- and stress activated protein kinase 1 significantly reduced cystatin mediated NF-kappaB dependent iNOS gene transcription suggesting the involvement of mitogen- and stress activated protein kinase 1 activation in ERK1/2 signaling
de Hemptinne et al., Mol Cell Biochem 2009 : Phosphorylation on Thr-106 and NO-modification of glyoxalase I suppress the TNF induced transcriptional activity of NF-kappaB ... Furthermore, we investigated the role of NO-mediated modification and phosphorylation of GLO1 in the TNF induced transcriptional activity of NF-kappaB ... Suppression of the basal and TNF induced NF-kappaB activity was significantly stronger upon expression of a GLO1 mutant that was either deficient for the NO-mediated modification or phosphorylation on Thr-106 ... However, upon overexpression of a GLO1 mutant that was deficient for both types of modification, the suppressive effect of GLO1 on TNF induced NF-kappaB activity was completely abolished ... However, upon overexpression of a GLO1 mutant that was deficient for both types of modification, the suppressive effect of GLO1 on TNF induced NF-kappaB activity was completely abolished ... These findings suggest that phosphorylation and NO-modification of glyoxalase I provides another control mechanism for modulating the basal and TNF induced expression of NF-kappaB-responsive genes
Lee et al., J Cell Biochem 2009 : H ( 2 ) O ( 2 ) increased NF-kappaB phosphorylation to induce an increase in the levels of cyclooxygenase (COX)-2 proteins ... H ( 2 ) O ( 2 ) increased NF-kappaB phosphorylation to induce an increase in the levels of cyclooxygenase (COX)-2 proteins
Noman et al., Innate Immun 2009 : Thalidomide prevented the activation of nuclear factor (NF)-KB by down regulating phosphorylation of inhibitory KB factor ( IKB ), and IKB kinase (IKK)-alpha and IKK-beta Moreover, thalidomide inhibited LPS induced phosphorylation of AKT, p38 and stress activated protein kinase ( SAPK ) /JNK ... Thalidomide prevented the activation of nuclear factor (NF)-KB by down regulating phosphorylation of inhibitory KB factor ( IKB ), and IKB kinase (IKK)-alpha and IKK-beta Moreover, thalidomide inhibited LPS induced phosphorylation of AKT , p38 and stress activated protein kinase ( SAPK ) /JNK ... Thalidomide prevented the activation of nuclear factor (NF)-KB by down regulating phosphorylation of inhibitory KB factor ( IKB ), and IKB kinase (IKK)-alpha and IKK-beta Moreover, thalidomide inhibited LPS induced phosphorylation of AKT, p38 and stress activated protein kinase ( SAPK ) /JNK ... Thalidomide prevented the activation of nuclear factor (NF)-KB by down regulating phosphorylation of inhibitory KB factor ( IKB ), and IKB kinase (IKK)-alpha and IKK-beta Moreover, thalidomide inhibited LPS induced phosphorylation of AKT, p38 and stress activated protein kinase ( SAPK ) /JNK
Kim et al., J Immunol 2009 : We also show that upon macrophage stimulation, p62 binds to TNFR associated factor 6, another E3 ligase important for NF-kappaB activation, but later this interaction was replaced by the recruitment of the deubiquitinating enzyme, cylindromatosis , an inhibitor of NF-kappaB activity
Kumar et al., J Immunol 2009 (Inflammation) : In this study, we tested the hypothesis that TAK1 ( TGF-beta activated kinase 1 ) is involved in TWEAK induced activation of NF-kappaB and MAPK and expression of proinflammatory protein ... In this study, we tested the hypothesis that TAK1 ( TGF-beta activated kinase 1 ) is involved in TWEAK induced activation of NF-kappaB and MAPK and expression of proinflammatory protein ... Furthermore, TWEAK induced transcriptional activation of NF-kappaB was significantly reduced in TAK1 ( -/- ) MEF and in C2C12 myoblasts transfected with a dominant negative TAK1 or TAK1 short interfering RNA
Sajan et al., J Lipid Res 2009 (Disease Models, Animal...) : In support of a critical mediatory role of aPKC, in both models, inhibition of hepatic aPKC by adenovirally mediated expression of kinase-inactive aPKC markedly diminished diet/insulin dependent activation of hepatic SREBP-1c and NFkappaB , and concomitantly improved hepatosteatosis, hypertriglyceridemia, hyperinsulinemia, and hyperglycemia
Lee et al., Nature 2009 (Alstrom Syndrome...) : Notably, the increased proliferation of basal keratinocytes is counterbalanced by the growth arrest of suprabasal keratinocytes in the stratified epidermis by IL1alpha dependent NFkappaB signalling
Huber et al., Hepatology 2009 (Reperfusion Injury) : The data provide strong evidence that the age related defect in hepatic NF-kappaB signaling during I/R is a result of decreased expression of PSMD4 , a proteasome subunit responsible for recognition and recruitment of ubiquitinylated substrates to the proteasome
Jo et al., J Dermatol 2009 (Melanosis) : Akt activation plays an important role in the activation of the transcription factor nuclear factor (NF)-kappaB and subsequent elevation of iNOS expression
Huang et al., J Mol Endocrinol 2009 : Urocortin induces interleukin-6 release from rat cardiomyocytes through p38 MAP kinase, ERK and NF-kappaB activation ... Urocortin induces interleukin-6 release from rat cardiomyocytes through p38 MAP kinase, ERK and NF-kappaB activation ... Ucn also activates nuclear factor kappa B (NF-kappaB) and a NF-kappaB inhibitor blocks Ucn induced IL-6 release ... Ucn also activates nuclear factor kappa B (NF-kappaB) and a NF-kappaB inhibitor blocks Ucn induced IL-6 release ... Finally, the CRH-R antagonists alpha-helical ( 9-41 ) CRH and astressin-2B completely inhibit Ucn induced IL-6 release, as well as activation of ERK, p38, and NF-kappaB ... Finally, the CRH-R antagonists alpha-helical ( 9-41 ) CRH and astressin-2B completely inhibit Ucn induced IL-6 release, as well as activation of ERK, p38, and NF-kappaB
Yao et al., J Neurosci 2009 : Blocking TRPC channels and specific downregulation of TRPC channels 1 and 5 resulted in suppression of CCL2 induced ERK/CREB activation but not Akt/NF-kappaB activation ... Blocking TRPC channels and specific downregulation of TRPC channels 1 and 5 resulted in suppression of CCL2 induced ERK/CREB activation but not Akt/NF-kappaB activation ... Blocking TRPC channels and specific downregulation of TRPC channels 1 and 5 resulted in suppression of CCL2 induced ERK/CREB activation but not Akt/NF-kappaB activation
Takeshima et al., BMC microbiology 2009 (Helicobacter Infections) : NF-kappaB activation by Helicobacter pylori requires Akt mediated phosphorylation of p65 ... The inflammatory response in Helicobacter pylori infected gastric tissue is mediated by cag pathogenicity island (PAI) dependent activation of nuclear factor-kappaB (NF-kappaB) ... PI3K inhibitor suppressed H. pylori induced p65 phosphorylation and NF-kappaB transactivation , as well as interleukin-8 expression ... PI3K inhibitor suppressed H. pylori induced p65 phosphorylation and NF-kappaB transactivation, as well as interleukin-8 expression
Li et al., Nan Fang Yi Ke Da Xue Xue Bao 2009 : Ang II stimulation of Kupffer cell results in increased activation of NF-kappaB via AT-1 alpha receptor
Kormann et al., Allergy 2009 (Genetic Predisposition to Disease...) : Functional effects of TLR2 variants were analyzed by NF-kappaB dependent luciferase reporter assay and interleukin-8 enzyme linked immunosorbent assay in vitro
Miller et al., Mol Cell Biol 1991 : In 70Z/3 cells, transforming growth factor beta , an inhibitor of kappa-gene induction, blocks the upregulation of Oct-2 but not the activation of NF-kappa B ... In 70Z/3 cells, transforming growth factor beta, an inhibitor of kappa-gene induction, blocks the upregulation of Oct-2 but not the activation of NF-kappa B
Kim et al., Clin Exp Immunol 2009 : BFT activated nuclear factor-kappaB (NF-kappaB) signals in HT-29 cells and pretreatment with eupatilin suppressed NF-kappaB activation that resulted in the significant inhibition of IL-8 and cyclo-oxygenase-2 expression ... In addition, herbimycin A, a specific inhibitor of heat shock protein 90 (Hsp90), decreased the BFT induced activation of IKK and NF-kappaB , suggesting that Hsp90 is associated with a pathway of IKK-NF-kappaB-IL-8/cyclo-oxygenase-2 gene signalling
Martinez-Losa et al., Clin Exp Allergy 2009 : The activation of eosinophil NF-kappaB induced by GM-CSF and IL-5 was suppressed by TauCl ... The activation of eosinophil NF-kappaB induced by GM-CSF and IL-5 was suppressed by TauCl
Wang et al., Biochem Biophys Res Commun 2009 : Previously, we have demonstrated evidence suggesting that SUMO4 negatively regulates NFkappaB transcriptional activity, probably through sumoylation of IkappaBalpha ... Luciferase reporter assays in 3T3 cells deficient for IkappaBalpha further demonstrated that SUMO4 regulates NFkappaB signaling dependent on its sumoylation of IkappaBalpha
Shinohara et al., Int Immunol 2009 : The serine/threonine kinase MEKK3 , also known as mitogen activated protein kinase kinase kinase 3, is a critical activator of the transcription factor NF-kappaB in innate immunity
Jin et al., Cancer Res 2009 (MAP Kinase Signaling System) : Thus, NEMO ubiquitination is a prerequisite for IKK dependent activation of NF-kappaB ... We found that inhibitors of apoptosis protein (IAP) regulate genotoxic stress induced NF-kappaB activation at different levels
Altomare et al., Proc Natl Acad Sci U S A 2009 (Mesothelioma) : We confirmed hemizygous loss of Faf1 and down-regulation of Faf1 protein in a series of MMs from Arf ( +/- ) mice, and we then showed that Faf1 regulates TNF-alpha mediated NF-kappaB signaling, a pathway previously implicated in asbestos induced oncogenesis of human mesothelial cells
Lee et al., Int J Obes Relat Metab Disord 2009 : Electrophoretic mobility shift assay confirmed that visfatin increased the DNA binding activity of NF-kappaB ... Our findings suggest that visfatin causes endothelial dysfunction by increasing inflammatory and adhesion molecule expression at least partly through the upregulation of NF-kappaB activity
László et al., Mol Cell Biochem 2009 : We illustrate a newly developed mechanism of eIF2alpha kinase mediated IkappaB depletion and subsequent NF-kappaB activation ... We illustrate a newly developed mechanism of eIF2alpha kinase mediated IkappaB depletion and subsequent NF-kappaB activation
Trakala et al., Eur J Immunol 2009 (Disease Susceptibility...) : LPS stimulation of p21-deficient macrophages led to increased IkappaBalpha kinase activity, and increased IkappaBalpha phosphorylation and degradation, resulting in elevated NF-kappaB activity
Wan et al., J Biol Chem 2009 (MAP Kinase Signaling System) : Although IRAK2 deficiency did not affect TLR4 mediated NFkappaB activation, a reduction of lipopolysaccharide (LPS) mediated mRNA stabilization contributed to the reduced cytokine and chemokine production observed in bone marrow derived macrophages from IRAK2-deficient mice ... Although IRAK2 deficiency did not affect TLR4 mediated NFkappaB activation, a reduction of lipopolysaccharide (LPS) mediated mRNA stabilization contributed to the reduced cytokine and chemokine production observed in bone marrow derived macrophages from IRAK2-deficient mice
Meyer-Schwesinger et al., American journal of physiology. Renal physiology 2009 (Nephritis...) : We therefore examined NF-kappaB signal transduction and found that NF-kappaB p65 phosphorylation and nuclear translocation were reduced by Rho kinase inhibition
Bansal et al., J Leukoc Biol 2009 (Tuberculosis) : The induced COX-2 expression in macrophages is dependent on NF-kappaB activation, which is mediated by inducible NO synthase (iNOS)/NO dependent participation of the members of Notch1-PI-3K signaling cascades as well as iNOS independent activation of ERK1/2 and p38 MAPKs
Nold-Petry et al., Proc Natl Acad Sci U S A 2009 : Increasing evidence demonstrates that interleukin (IL)-32 is a pro-inflammatory cytokine, inducing IL-1alpha, IL-1beta, IL-6, tumor necrosis factor (TNF)-alpha, and chemokines via nuclear factor (NF)-kappaB , p38 mitogen activated protein kinase ( MAPK ), and activating protein (AP)-1 activation
Wang et al., Haematologica 2009 : Bay 11-7082, a pharmacological inhibitor of NF-kappaB activation, also reduced NKp46 expression and suppressed redirected cytotoxicity
Hastings et al., Arterioscler Thromb Vasc Biol 2009 (Atherosclerosis) : Neutralization of the IL-8 receptor, CXCR2, further induced VCAM-1 in the presence of IL-1beta, and phospho-p38 was required for NF-kappaB activation and VCAM-1 expression ... Neutralization of the IL-8 receptor, CXCR2, further induced VCAM-1 in the presence of IL-1beta, and phospho-p38 was required for NF-kappaB activation and VCAM-1 expression ... Neutralization of the IL-8 receptor, CXCR2, further induced VCAM-1 in the presence of IL-1beta , and phospho-p38 was required for NF-kappaB activation and VCAM-1 expression ... Additionally, IL-8 reduced p38 activation and NF-kappaB activity induced by IL-1beta alone ... Additionally, IL-8 reduced p38 activation and NF-kappaB activity induced by IL-1beta alone
Lo et al., J Cell Physiol 2009 : These cells were then stimulated with a high glucose concentration to modulate ANP, TGF-beta1, collagen type I, NF-kappaB and IkappaB-alpha, and the results showed that ANP, TGF-beta1, collagen type I and NF-kappaB significantly increased in untransfected cells, and the transfection of ANP significantly attenuated high glucose activated TGF-beta1 , collagen I and NF-kappaB expression ... These cells were then stimulated with a high glucose concentration to modulate ANP, TGF-beta1, collagen type I, NF-kappaB and IkappaB-alpha, and the results showed that ANP , TGF-beta1, collagen type I and NF-kappaB significantly increased in untransfected cells, and the transfection of ANP significantly attenuated high glucose activated TGF-beta1, collagen I and NF-kappaB expression
Tomita et al., Int J Cancer 2009 (Leukemia-Lymphoma, Adult T-Cell) : Transfection of Aurora A expression plasmid enhanced Tax induced nuclear factor-kappaB (NF-kappaB) reporter activity
Goto et al., Cytokine 2009 : IL-13 induces translocation of NF-kappaB in cultured human bronchial smooth muscle cells ... In the present study, whether IL-13 induces an activation of nuclear factor (NF)-kappaB , inflammatory transcription factor, was investigated in human bronchial smooth muscle cells ( hBSMCs )
Park et al., J Neuroimmunol 2009 (Encephalitis...) : Furthermore, KRG and GTS significantly suppressed NF-kappaB and MAP kinase activities, which are upstream signaling molecules in inflammation
Minoda et al., Biochem Biophys Res Commun 2009 : Overexpression of FBXW5 inhibited IL-1beta induced activation of JNK/p38 MAPKs and NF-kappaB as well as phosphorylation of TAK1 on Thr187. Conversely, knockdown of FBXW5 resulted in the prolonged activation of TAK1 upon IL-1beta stimulation ... Overexpression of FBXW5 inhibited IL-1beta induced activation of JNK/p38 MAPKs and NF-kappaB as well as phosphorylation of TAK1 on Thr187. Conversely, knockdown of FBXW5 resulted in the prolonged activation of TAK1 upon IL-1beta stimulation
Shin et al., Biochem Biophys Res Commun 2009 (Disease Models, Animal...) : In addition, chrysin inhibited tumor necrosis factor (TNF)-alpha induced activation of NF-kappaB in IEC-6 cells
Jung et al., Food Chem Toxicol 2009 (Inflammation) : The molecular mechanisms that underlie GHE mediated attenuation are related to the inhibition of the phosphorylation of three mitogen activated protein kinases ( MAPKs ), extracellular signal regulated kinases 1 and 2 ( ERK1/2 ), p38 MAPK , and c-Jun N-terminal kinase (JNK), and the activation of nuclear factor-kappaB (NF-kappaB) ... The molecular mechanisms that underlie GHE mediated attenuation are related to the inhibition of the phosphorylation of three mitogen activated protein kinases ( MAPKs ), extracellular signal regulated kinases 1 and 2 ( ERK1/2 ), p38 MAPK, and c-Jun N-terminal kinase (JNK) , and the activation of nuclear factor-kappaB (NF-kappaB)
Romier-Crouzet et al., Food Chem Toxicol 2009 (Hemolysis) : The effect of polyphenolic extracts, at 50 micromol of gallic acid equivalent/l, was investigated on inflammation related cellular events : ( i) NF-kappaB activity ( cells transfected with a NF-kappaB-luciferase construct ), ( ii ) activation of Erk1/2 and JNK ( western blotting ), ( iii ) secretion of interleukin 8 (IL-8) ( ELISA ), ( iv ) secretion of prostaglandin ( PG ) E ( 2 ) ( ELISA ), ( v ) production of NO ( Griess method ) ... The effect of polyphenolic extracts, at 50 micromol of gallic acid equivalent/l, was investigated on inflammation related cellular events : ( i) NF-kappaB activity ( cells transfected with a NF-kappaB-luciferase construct ), ( ii ) activation of Erk1/2 and JNK ( western blotting ), ( iii ) secretion of interleukin 8 (IL-8) ( ELISA ), ( iv ) secretion of prostaglandin ( PG ) E ( 2 ) ( ELISA ), ( v ) production of NO ( Griess method )
Lin et al., Cardiovasc Res 2009 : Our results suggest that in VSMCs, activation of p42/p44 MAPK, p38 MAPK, and NF-kappaB is essential for ATPgammaS induced COX-2 expression and PGE2 synthesis
Andrews et al., J Immunol 2009 : T1, in contrast to follicular mature B cells, failed to express key NF-kappaB target genes in response to BCR engagement and exhibited a striking defect in assembly of an active transcriptional complex at the promoter of the survival and proliferative genes A1 and c-Myc
Zhang et al., Invest Ophthalmol Vis Sci 2009 (Diabetes Mellitus, Experimental...) : Analysis of downstream signals showed that inhibition of NAD ( P ) H oxidase partially inhibited NF-kappaB activation but did not reduce CCL2 mRNA stability or prevent TNF-alpha induced phosphorylation of p38MAPK
Garrison et al., Oncogene 2009 : Here, we investigated the role of TRAF2 in c-IAP2/MALT1 induced NF-kappaB activation ... Here, we investigated the role of TRAF2 in c-IAP2/MALT1 induced NF-kappaB activation ... Here, we investigated the role of TRAF2 in c-IAP2/MALT1 induced NF-kappaB activation ... Studies of c-IAP2/MALT1 BIR1 mutant ( E47A/R48A ) that fails to activate NF-kappaB showed loss of TRAF2 binding, but retention of TRAF6 binding, suggesting that interaction of c-IAP2/MALT1 with TRAF6 is insufficient for NF-kappaB induction ... Studies of c-IAP2/MALT1 BIR1 mutant ( E47A/R48A ) that fails to activate NF-kappaB showed loss of TRAF2 binding, but retention of TRAF6 binding, suggesting that interaction of c-IAP2/MALT1 with TRAF6 is insufficient for NF-kappaB induction ... Studies of c-IAP2/MALT1 BIR1 mutant ( E47A/R48A ) that fails to activate NF-kappaB showed loss of TRAF2 binding, but retention of TRAF6 binding, suggesting that interaction of c-IAP2/MALT1 with TRAF6 is insufficient for NF-kappaB induction ... In addition, a dominant negative TRAF2 mutant or downregulation of TRAF2 achieved by small interfering RNA inhibited NF-kappaB activation by c-IAP2/MALT1 showing that TRAF2 is indispensable ... Comparisons of the bioactivity of intact c-IAP2/MALT1 oncoprotein and BIR1 E47A/R48A c-IAP2/MALT1 mutant that can not bind TRAF2 in a lymphoid cell line provided evidence that TRAF2 interaction is critical for c-IAP2/MALT1 mediated increases in the NF-kappaB activity, increased expression of endogenous NF-kappaB target genes ( c-FLIP, TRAF1 ), and resistance to apoptosis ... Comparisons of the bioactivity of intact c-IAP2/MALT1 oncoprotein and BIR1 E47A/R48A c-IAP2/MALT1 mutant that can not bind TRAF2 in a lymphoid cell line provided evidence that TRAF2 interaction is critical for c-IAP2/MALT1 mediated increases in the NF-kappaB activity, increased expression of endogenous NF-kappaB target genes ( c-FLIP, TRAF1 ), and resistance to apoptosis ... Comparisons of the bioactivity of intact c-IAP2/MALT1 oncoprotein and BIR1 E47A/R48A c-IAP2/MALT1 mutant that can not bind TRAF2 in a lymphoid cell line provided evidence that TRAF2 interaction is critical for c-IAP2/MALT1 mediated increases in the NF-kappaB activity, increased expression of endogenous NF-kappaB target genes ( c-FLIP, TRAF1 ), and resistance to apoptosis
Joo et al., PloS one 2009 (Colitis...) : Murine splenocytes and intestinal epithelial cells were used to determine the in vitro impact of TLE on LPS induced NF-kappaB signaling ... In vitro, TLE blocked LPS induced IkappaBalpha degradation, RelA translocation, NF-kappaB transcriptional activity and MIP-2 mRNA accumulation in IEC-18 cells ... In vitro, TLE blocked LPS induced IkappaBalpha degradation, RelA translocation, NF-kappaB transcriptional activity and MIP-2 mRNA accumulation in IEC-18 cells
Teng et al., Inflammation 2009 (Colitis) : The main aims of this study is to evaluate effects of intraperitoneal injection recombinant human TFF3 on the expression of tumour necrosis factor alpha (TNF-alpha), toll-like receptor 4 ( TLR4 ), and nuclear factor kappaB (NF-kappaB) in trinitrobenzene sulphonic acid ( TNBS ) induced colitis mice
Chung et al., Br J Pharmacol 2009 : The ERK activation, NF-kB translocation and PDGF-BB production were blocked by PP2 , U73122 and PD98059
Gu et al., Nature 2009 (Cystic Fibrosis...) : Neutrophils, but not macrophages, from Ifrd1-deficient mice showed blunted effector function, associated with decreased NF-kappaB p65 transactivation
Joyce et al., PLoS Pathog 2009 (Hepatitis C) : We conclude that HCV contributes to hepatocyte damage and apoptosis by inducing stress and pro-apoptotic BAX while preventing the induction of anti-apoptotic NF-kappaB and BCL-xL, thus sensitizing hepatocytes to apoptosis
Dai et al., J Cell Mol Med 2010 : We report here that HSP70 over-expression in human colon cancer cells can inhibit TNFalpha induced NFkappaB activation but promote TNFalpha induced activation of c-Jun N-terminal kinase (JNK) through interaction with TNF receptor (TNFR) associated factor 2 ( TRAF2 ) ... We provide evidence that HSP70 over-expression can sequester TRAF2 in detergent-soluble fractions possibly through interacting with TRAF2, leading to reduced recruitment of receptor interacting protein ( RIP1 ) and IkappaB alpha kinase (IKK) signalosome to the TNFR1-TRADD complex and inhibited NFkappaB activation after TNFalpha stimuli ... Therefore, our study suggests that HSP70 may differentially regulate TNFalpha induced activation of NFkappaB and JNK through interaction with TRAF2, contributing to the pro-apoptotic roles of HSP70 in TNFalpha induced apoptosis of human colon cancer cells ... Therefore, our study suggests that HSP70 may differentially regulate TNFalpha induced activation of NFkappaB and JNK through interaction with TRAF2 , contributing to the pro-apoptotic roles of HSP70 in TNFalpha induced apoptosis of human colon cancer cells
Liang et al., J Cell Mol Med 2009 (Inflammation) : Based on the screening results of these analogues, five active compounds A01, A03, A13, B18 and C22 were investigated to inhibit TNF-alpha and IL-6 release in a dose dependent manner, three of which further demonstrated inhibitory effects on LPS induced TNF-alpha, IL-1beta, IL-6, MCP-1, COX-2, PGES, iNOS and p65 NF-kappaB mRNA production
Hong et al., J Exp Clin Cancer Res 2009 (Carcinoma, Squamous Cell...) : All of these findings suggest that Akt inhibition could induce the MErT through decreased NF-kappaB signaling and downregulation of Snail and Twist in OSCC cells
Voegeli et al., Inflamm Res 2009 : Angiotensin II (Ang II) induces the proinflammatory nuclear factor kappaB (NF-kappaB) in the vasculature ... The proinflammatory effects of Ang II on NF-kappaB in vascular smooth muscle cells may be through a non-canonical pathway and be dependent on p65 phosphorylation ... The proinflammatory effects of Ang II on NF-kappaB in vascular smooth muscle cells may be through a non-canonical pathway and be dependent on p65 phosphorylation
Wehling et al., Arthritis Rheum 2009 : Matrix metalloproteinase 13 was induced by IL-1beta in a NF-kappaB dependent manner ... Matrix metalloproteinase 13 was induced by IL-1beta in a NF-kappaB dependent manner
Hamid et al., Circulation 2009 (Cardiomegaly...) : In H9c2 cardiomyocytes, sustained NF-kappaB activation was proapoptotic, an effect dependent on TNFR1 signaling, whereas TNFR2 overexpression attenuated TNF induced NF-kappaB activation ... In H9c2 cardiomyocytes, sustained NF-kappaB activation was proapoptotic, an effect dependent on TNFR1 signaling, whereas TNFR2 overexpression attenuated TNF induced NF-kappaB activation
Mulla et al., Am J Reprod Immunol 2009 : In addition, Nod1 and Nod2 mRNA expression was upregulated following treatment of trophoblast cells with lipopolysaccharide (LPS), and this was significantly reduced by the presence of a NFkappaB inhibitor and a TLR4-dominant negative ( DN ) ... In addition, Nod1 and Nod2 mRNA expression was upregulated following treatment of trophoblast cells with lipopolysaccharide (LPS), and this was significantly reduced by the presence of a NFkappaB inhibitor and a TLR4-dominant negative ( DN )
Yang et al., EMBO J 2009 : Depletion of Set9 by siRNA or mutation of the RelA methylation sites prolongs DNA binding of NF-kappaB and enhances TNF-alpha induced expression of NF-kappaB target genes
Zhou et al., Mol Carcinog 2009 (Esophageal Neoplasms...) : In this article, we found that Degenerative Spermatocyte Homolog 1 ( DEGS1 ) up-regulated the expression of cyclin D1 and the activation of transcription factor NF-kappaB was essential for DEGS1 induced cyclin D1 production
Chen et al., Science 2009 (Inflammation...) : CD24 associates with high mobility group box 1, heat shock protein 70, and heat shock protein 90 ; negatively regulates their stimulatory activity ; and inhibits nuclear factor kappaB (NF-kappaB) activation
Manukyan et al., J Immunol 2009 : Both Src family kinases and RhoA were required for NF-kappaB activation, whereas RhoA was dispensable for type I IFN generation
Théâtre et al., Am J Respir Cell Mol Biol 2009 (Calcium Signaling) : Although these agonists did not induce significant IL-8 gene expression by these cells, they markedly enhanced TNF-alpha induced NF-kappaB activation , resulting in increased IL-8 expression and release ... Although these agonists did not induce significant IL-8 gene expression by these cells, they markedly enhanced TNF-alpha induced NF-kappaB activation, resulting in increased IL-8 expression and release
Seo et al., Eur J Immunol 2009 : Instead, NF-kappaB overexpression increased the expression of Smad7 transcripts
Wu et al., Inflamm Res 2009 : Ang II upregulates the expression of TLR4 by RPMCs, resulting in enhanced NF-kappaB signaling and induction of CD40, TNF-alpha , and IL-6 expression ... Ang II upregulates the expression of TLR4 by RPMCs, resulting in enhanced NF-kappaB signaling and induction of CD40 , TNF-alpha, and IL-6 expression ... Ang II upregulates the expression of TLR4 by RPMCs, resulting in enhanced NF-kappaB signaling and induction of CD40, TNF-alpha, and IL-6 expression
Annabi et al., Journal of neuroinflammation 2009 (Glioblastoma) : We provide evidence for enhanced COX-2 expression in CD133 ( + ) glioma cells, and direct cell based evidence of NF-kappaB mediated COX-2 regulation by MT1-MMP
Kim et al., Frontiers in bioscience (Landmark edition) 2009 (Diabetes Mellitus, Type 1) : By employing TNF-alpha / IFN-gamma synergism model which causes beta -cell apoptosis, we found that the antiapoptotic X-linked inhibitor of apoptosis (XIAP) molecule is upregulated by NF-kappaB in response to TNF-alpha and XIAP induction was inhibited by IFN-gamma induced signal transducer and activator of transcription-1 ( STAT1 ) activation, which explains the death of beta -cells by TNF-alpha /IFN-gamma synergism ... By employing TNF-alpha / IFN-gamma synergism model which causes beta -cell apoptosis, we found that the antiapoptotic X-linked inhibitor of apoptosis (XIAP) molecule is upregulated by NF-kappaB in response to TNF-alpha and XIAP induction was inhibited by IFN-gamma induced signal transducer and activator of transcription-1 ( STAT1 ) activation, which explains the death of beta -cells by TNF-alpha /IFN-gamma synergism
Hu et al., Frontiers in bioscience (Landmark edition) 2009 (Cardiomegaly) : TAC significantly reduced the association of Tollip with IRAK-1 by 66.4 percent and increased NF-kappaB binding activity by 86.5 percent and the levels of phospho-p38 by 114.6 percent in the myocardium compared with sham control, respectively ... In vitro experiments showed that IL-1beta stimulation also significantly reduced the association of Tollip with IRAK-1 and increased NF-kappaB binding activity in neonatal cardiomyocytes ... Overexpression of Tollip also reduced NF-kappaB binding activity by 30.7 percent and phospho-p38 by 47.1 percent, respectively
Kim et al., Frontiers in bioscience (Landmark edition) 2009 (Myocardial Infarction) : Activation of nuclear factor kappa B (NF-kappa B) by TNF-alpha , up-regulates the expression of molecules which are involved in inflammation and cell adhesion
Cao et al., Inflammation 2009 (Inflammation) : Pep 3 ( LRENECVS ) which was derived from the hydrophilic region of A1 module in CRD4 remarkably inhibited the binding of TNF-alpha to TNF-R1, and also reversed TNF-alpha induced degradation of IkappaB-alpha and nuclear translocation of NF-kappaB p65 subunit in HeLa cells
Zordoky et al., Curr Drug Metab 2009 (Inflammation) : First, NF-kappaB can directly regulate the expression of CYP1A1 , CYP2B1/2, CYP2C11, CYP2D5, CYP2E1, CYP3A7, and CYP27B1 through binding to the promoter region of these genes ... First, NF-kappaB can directly regulate the expression of CYP1A1, CYP2B1/2, CYP2C11, CYP2D5, CYP2E1, CYP3A7, and CYP27B1 through binding to the promoter region of these genes ... First, NF-kappaB can directly regulate the expression of CYP1A1, CYP2B1/2 , CYP2C11, CYP2D5, CYP2E1, CYP3A7, and CYP27B1 through binding to the promoter region of these genes ... First, NF-kappaB can directly regulate the expression of CYP1A1, CYP2B1/2, CYP2C11 , CYP2D5, CYP2E1, CYP3A7, and CYP27B1 through binding to the promoter region of these genes ... First, NF-kappaB can directly regulate the expression of CYP1A1, CYP2B1/2, CYP2C11, CYP2D5 , CYP2E1, CYP3A7, and CYP27B1 through binding to the promoter region of these genes ... First, NF-kappaB can directly regulate the expression of CYP1A1, CYP2B1/2, CYP2C11, CYP2D5, CYP2E1 , CYP3A7, and CYP27B1 through binding to the promoter region of these genes ... First, NF-kappaB can directly regulate the expression of CYP1A1, CYP2B1/2, CYP2C11, CYP2D5, CYP2E1, CYP3A7 , and CYP27B1 through binding to the promoter region of these genes ... First, NF-kappaB can directly regulate the expression of CYP1A1, CYP2B1/2, CYP2C11 , CYP2D5, CYP2E1, CYP3A7, and CYP27B1 through binding to the promoter region of these genes
Madhusoodhanan et al., Cancer Biol Ther 2009 (Adenocarcinoma...) : Furthermore, FIR induced NFkappaB-DNA binding activity and IkappaBalpha phosphorylation
Chen et al., Inflamm Res 2009 : Sulforaphane had no effect on TNF-alpha induced NF-kappaB nuclear binding activity, IkappaB-alpha degradation or activation of NF-kappaB-driven transcriptional activity ... Sulforaphane had no effect on TNF-alpha induced NF-kappaB nuclear binding activity, IkappaB-alpha degradation or activation of NF-kappaB-driven transcriptional activity
Ganta et al., Mol Pharm 2009 : In this study, we have examined augmentation of therapeutic efficacy upon coadministration of paclitaxel ( PTX ) and curcumin ( CUR ), an inhibitor of nuclear factor kappa B (NFkappaB) as well as a potent down-regulator of ABC transporters, in wild-type SKOV3 and drug resistant SKOV3 ( TR ) human ovarian adenocarcinoma cells
Warstat et al., Ann Rheum Dis 2010 (Arthritis, Rheumatoid...) : This IL16 regulation involved p38MAPK, ERK1/2 and SMAD2 signalling, but not NFkappaB
Demetriades et al., J Virol 2009 : The LMP1 promoter can be transactivated directly by NF-kappaB
Cheng et al., Free Radic Biol Med 2009 : Induction of cPLA2 by CSE was attenuated by selective inhibitors of NF-kappaB ( helenalin ) and AP-1 ( curcumin )
Luo et al., Toxicol Appl Pharmacol 2009 (Second Messenger Systems) : Activation of ROS/NF-kappaB and Ca2+/CaM kinase II are necessary for VCAM-1 induction in IL-1beta treated human tracheal smooth muscle cells ... Moreover, IL-1beta stimulated NF-kappaB and CaMKII phosphorylation through MyD88 dependent PI-PLC/PKCalpha/c-Src/ROS and PI-PLC/Ca2+/CaM pathways, respectively ... Moreover, IL-1beta stimulated NF-kappaB and CaMKII phosphorylation through MyD88 dependent PI-PLC/PKCalpha/c-Src/ROS and PI-PLC/Ca2+/CaM pathways, respectively
Ming et al., BMC cardiovascular disorders 2009 : Moreover, the inhibitory effect of L-norvaline was not reversed by the NOS inhibitor L-NAME and L-norvaline did not interfere with TNFalpha induced activation of NF-kappaB , JNK, p38mapk , while it inhibited p70s6k ( S6K1 ) activity ... Moreover, the inhibitory effect of L-norvaline was not reversed by the NOS inhibitor L-NAME and L-norvaline did not interfere with TNFalpha induced activation of NF-kappaB , JNK, p38mapk, while it inhibited p70s6k ( S6K1 ) activity
Lin et al., J Med Chem 2009 (Lung Neoplasms) : We further confirmed that PBT-1 suppressed Akt activation accelerated RelA degradation via IkappaB kinase-alpha and down-regulated NF-kappaB target gene expression
Wang et al., Zhong Xi Yi Jie He Xue Bao 2009 : TNF-alpha ( 1 microg/L ) strongly induced the expression of NF-kappaB by approximately 1.76-fold compared with the control in the nuclei of A549 cells, and the induced NF-kappaB expression was significantly suppressed by addition of Feiyanning ( P < 0.01 ). In addition, Feiyanning inhibited the transcriptional activity of the NF-kappaB promoter. However, we observed no significant changes in IkappaBalpha expression ( P > 0.05 ) ... Feiyanning Decoction can markedly inhibit human lung cancer A549 cell proliferation, which may be partly due to inhibition of NF-kappaB activation induced by TNF-alpha
Mendes et al., Cell Signal 2009 : Rapamycin also enhanced the LPS induced NF-kappaB transactivation as determined by a reporter assay, phosphorylation of the p38 and Erk1/2 MAPKs, and counteracted PPAR activity
Zhang et al., Neuroscience 2009 (Alzheimer Disease) : Our findings suggest that NF-kappaB can mediate induction of COX-2, TNFalpha and astrogliosis in APPswe/PS1dE9 mice ... Our findings suggest that NF-kappaB can mediate induction of COX-2 , TNFalpha and astrogliosis in APPswe/PS1dE9 mice
Ju et al., Exp Mol Med 2009 (AIDS Dementia Complex...) : Taken together, our results indicate that HIV-1 Tat can up-regulate expression of MMP-9 via MAPK-NF-kappaB dependent mechanisms as well as Tat induced TNF-alpha production in astrocytes ... Taken together, our results indicate that HIV-1 Tat can up-regulate expression of MMP-9 via MAPK-NF-kappaB dependent mechanisms as well as Tat induced TNF-alpha production in astrocytes
Wicovsky et al., Oncogene 2009 : It has been shown that tumor necrosis factor receptor-2 (TNFR2) stimulation leads to degradation of TNF receptor associated factor-2 (TRAF2) and inhibition of TNFR1 induced activation of NFkappaB and JNK ... It has been shown that tumor necrosis factor receptor-2 (TNFR2) stimulation leads to degradation of TNF receptor associated factor-2 (TRAF2) and inhibition of TNFR1 induced activation of NFkappaB and JNK ... Here, we show that TRAF1 inhibits TNFR2 induced proteasomal degradation of TRAF2 and relieves TNFR1 induced activation of NFkappaB from the inhibitory effect of TNFR2 ... Despite lacking an amino-terminal RING/zinc-finger domain, TRAF1 did not interfere with TNFR1 induced activation of JNK and NFkappaB ... It is noted that physiological expression levels of TRAF1 enhanced NFkappaB activation and interleukin-8 (IL8) production induced by TNFR2
Bren et al., PloS one 2009 : Gp120 induced caspase 8 dependent NF-kappaB activation is a novel pathway of HIV replication which increases understanding of the biology of T-cell death, as well as having implications for understanding treatment and prevention of HIV infection
Shan et al., Int J Oncol 2009 (Urinary Bladder Neoplasms) : Sulforaphane down-regulates COX-2 expression by activating p38 and inhibiting NF-kappaB-DNA binding activity in human bladder T24 cells ... Furthermore, SB202190 alone induced NF-kappaB translocation to the nucleus, promoted NF-kappaB binding to the COX-2 promoter and resulted in up-regulation of COX-2 expression
Gadgeel et al., Cancer 2009 (Carcinoma, Non-Small-Cell Lung...) : Akt activates nuclear factor kappa B (NF-kappaB) , which transcribes genes important for cell survival, invasion, and metastasis
Kurada et al., J Biol Chem 2009 (Neoplasms) : We investigated the physiological role of endogenous MAPK activating death domain containing protein ( MADD ), a splice variant of the IG20 gene, that can interact with TNFR1 in tumor necrosis factor-alpha (TNFalpha) induced activation of NF-kappaB , MAPK, ERK1/2, JNK, and p38
Bazzoni et al., Proc Natl Acad Sci U S A 2009 (Inflammation) : Among the 3 different genes encoding miR-9 precursors in humans, we show that LPS selectively induces the transcription of miR-9-1 located in the CROC4 locus, in a MyD88- and NF-kappaB dependent manner ... Among the 3 different genes encoding miR-9 precursors in humans, we show that LPS selectively induces the transcription of miR-9-1 located in the CROC4 locus, in a MyD88- and NF-kappaB dependent manner ... Overall, these data suggest that TLR4 activated NF-kappaB rapidly increases the expression of miR-9 that operates a feedback control of the NF-kappaB dependent responses by fine tuning the expression of a key member of the NF-kappaB family
Bansal et al., PloS one 2009 : PIM2 triggered significant p65 nuclear factor -kappaB (NF-kappaB) nuclear translocation that was dependent on activation of PI3K or Notch1 signaling
Tchivileva et al., Molecular pain 2009 : Characterization of NF-kB mediated inhibition of catechol-O-methyltransferase ... Cell culture assays and functional deletion analyses of the cloned P2-COMT promoter demonstrate that TNFalpha inhibits P2-COMT activity in astrocytes by inducing NF-kappaB complex recruitment to the specific kappaB binding site ... Collectively, our findings provide the first evidence for NF-kappaB mediated inhibition of COMT expression in the central nervous system, suggesting that COMT contributes to the pathogenesis of inflammatory pain states
Furusawa et al., Int Immunopharmacol 2009 : On the other hand, Licochalcone C, Echinatin and Isoliquitigenin failed to inhibit LPS induced NF-kappaB activation
Stuhlmeier et al., Exp Gerontol 2009 (Arthritis) : Data are presented that show that the effects of HT on p38 are clearly independent of HT effects on NF-kappaB
Min et al., Int Immunopharmacol 2009 : In addition, 5-hydroxyzerumbone inhibited LPS induced transcriptional activation of NF-kappaB , indicating that regulation of NF-kappaB activity might be involved in the inhibition of NO production by 5-hydroxyzerumbone ... Taken together, these results suggest that down-regulation of LPS induced NO production by 5-hydroxyzerumbone is mediated by the suppression of iNOS expression through the modulation of NF-kappaB activation and HO-1 induction in macrophage cells
Kok et al., Trends Biochem Sci 2009 (Neoplasms) : Recently identified mechanisms that control PI3K production include increased gene copy number in cancer, and transcriptional regulation of the p110alpha PI3K gene by FOXO3a, NF-kappaB and p53, and of the PI3K regulatory subunits by STAT3, EBNA-2 and SREBP ... Recently identified mechanisms that control PI3K production include increased gene copy number in cancer, and transcriptional regulation of the p110alpha PI3K gene by FOXO3a, NF-kappaB and p53, and of the PI3K regulatory subunits by STAT3, EBNA-2 and SREBP
Kang et al., Cytokine 2009 : Induction of IL-12 by LMM in peritoneal macrophages involved NF-kappaB , p38 and JNK, but not ERK1/2
Shen et al., Am J Physiol Gastrointest Liver Physiol 2009 (Liver Diseases, Alcoholic) : Taken together, our findings suggest that SIRT1-NF-kappaB signaling is involved in regulating LPS- and metabolites-of-ethanol mediated TNF-alpha production in rat Kupffer cells and in murine macrophages
Benitez et al., Prostate 2009 (Prostatic Neoplasms) : RES induced a dose dependent cytoplasmic retention of NF kappaB mediated by I kappaB alpha in PC-3 cells but not in LNCaP
N'Diaye et al., Life Sci 2009 (Atherosclerosis) : TNFalpha- and NF-kappaB dependent induction of the chemokine CCL1 in human macrophages exposed to the atherogenic lipoprotein ( a ) ... DNA binding activity of NF-kappaB , a well-known molecular effector of TNFalpha, was moreover activated by Lp(a) in a TNFalpha dependent manner and the use of the NF-kappaB inhibitor Bay 11-7082 blocked Lp(a) triggered CCL1 induction ... DNA binding activity of NF-kappaB , a well-known molecular effector of TNFalpha, was moreover activated by Lp(a) in a TNFalpha dependent manner and the use of the NF-kappaB inhibitor Bay 11-7082 blocked Lp(a) triggered CCL1 induction ... In addition, Lp(a) induced binding of NF-kappaB to a NF-kappaB consensus element on CCL1 promoter as assessed by EMSA ... These data suggest that Lp(a) triggered induction of CCL1 expression is mediated by TNFalpha and subsequent activation of NF-kappaB , without AhR involvement
Wu et al., Cancer Lett 2009 (Carcinoma, Hepatocellular...) : Inhibition of NF-kappaB activity significantly reduced proliferation and invasion of Hep3B cells as well as down-regulated the expression of invasion related molecules including matrix metalloproteinase (MMP)-2, MMP-9, membrane type-1 MMP (MT1-MMP), urokinase plasminogen activator (uPA) and vascular endothelial growth factor ( VEGF ) ... Inhibition of NF-kappaB activity significantly reduced proliferation and invasion of Hep3B cells as well as down-regulated the expression of invasion related molecules including matrix metalloproteinase (MMP)-2, MMP-9, membrane type-1 MMP (MT1-MMP) , urokinase plasminogen activator (uPA) and vascular endothelial growth factor ( VEGF ) ... Inhibition of NF-kappaB activity significantly reduced proliferation and invasion of Hep3B cells as well as down-regulated the expression of invasion related molecules including matrix metalloproteinase (MMP)-2 , MMP-9, membrane type-1 MMP (MT1-MMP), urokinase plasminogen activator (uPA) and vascular endothelial growth factor ( VEGF ) ... Inhibition of NF-kappaB activity significantly reduced proliferation and invasion of Hep3B cells as well as down-regulated the expression of invasion related molecules including matrix metalloproteinase (MMP)-2, MMP-9 , membrane type-1 MMP (MT1-MMP), urokinase plasminogen activator (uPA) and vascular endothelial growth factor ( VEGF ) ... Inhibition of NF-kappaB activity significantly reduced proliferation and invasion of Hep3B cells as well as down-regulated the expression of invasion related molecules including matrix metalloproteinase (MMP)-2, MMP-9, membrane type-1 MMP (MT1-MMP) , urokinase plasminogen activator (uPA) and vascular endothelial growth factor ( VEGF ) ... Inhibition of NF-kappaB activity significantly reduced proliferation and invasion of Hep3B cells as well as down-regulated the expression of invasion related molecules including matrix metalloproteinase (MMP)-2, MMP-9, membrane type-1 MMP (MT1-MMP), urokinase plasminogen activator (uPA) and vascular endothelial growth factor ( VEGF )
Lu et al., Am J Physiol Heart Circ Physiol 2009 (Myocardial Reperfusion Injury) : In addition to functional measures of left ventricular developed and end-diastolic pressure, phosphorylation of IkappaBalpha and activation of NF-kappaB were measured along with cytosolic protein content of Bcl-2, release of cytochrome c, and degradation of caspase-3 ... APC resulted in ROS dependent phosphorylation of IkappaBalpha and activation of NF-kappaB before I/R. APC also increased the expression of Bcl-2 before I/R ... APC resulted in ROS dependent phosphorylation of IkappaBalpha and activation of NF-kappaB before I/R. APC also increased the expression of Bcl-2 before I/R ... These protective effects of APC were abolished by transient inhibition of IkappaBalpha phosphorylation and NF-kappaB activation by SC-514 followed by washout ... ROS dependent activation of NF-kappaB by APC before I/R is a critical element in the protective effect of APC
Kannan et al., PloS one 2009 (Pseudomonas Infections) : Mechanistically, we found that Lyn mediated NFkappaB activation led to increased gene expression and secretion of MCP-1 ... Mechanistically, we found that Lyn mediated NFkappaB activation led to increased gene expression and secretion of MCP-1
Wang et al., J Cell Biochem 2009 (Orthostatic Intolerance) : These findings indicate that clinorotation upregulates iNOS in HUVECs by a mechanism dependent on suppression of AP-1 , but not NF-kappaB
Kim et al., Bone 2009 (Bone Resorption) : In addition, HSP60 activated p38 and NFkappaB , but not ERK or JNK ; importantly, inhibitors of p38 ( SB203580 ) and NFkappaB ( PDTC ) abolished HSP60 induced apoptosis ... Furthermore, Western blotting showed that HSP60 up-regulated TLR-2 and TLR-4 expression, and pretreatment with blocking antibodies for TLR-2 and TLR-4 almost completely eliminated the effects of HSP60 on apoptosis, caspase-3 and -9 activation, and activation of NFkappaB and p38 MAPK ... Furthermore, Western blotting showed that HSP60 up-regulated TLR-2 and TLR-4 expression, and pretreatment with blocking antibodies for TLR-2 and TLR-4 almost completely eliminated the effects of HSP60 on apoptosis, caspase-3 and -9 activation, and activation of NFkappaB and p38 MAPK
Suzuki et al., Am J Respir Cell Mol Biol 2009 : NF-kappaB inhibitors also reduced p53 phosphorylation
Lomonaco et al., DNA Cell Biol 2009 (Xeroderma Pigmentosum) : Given the important function of NF-kappaB in regulating transcription of the bcl-x ( l ) gene and the Bcl-x ( L ) protein in preventing apoptosis, these results suggest that NER may protect cells against cisplatin induced apoptosis by activating NF-kappaB , which further induces transcription of the bcl-x ( l ) gene, resulting in an accumulation of Bcl-x ( L ) protein and activation of the cell survival pathway that leads to increased cell survival under cisplatin treatment
Zhang et al., Cell Prolif 2009 (Carcinoma, Adenoid Cystic...) : NF-kappaB promotes iNOS and VEGF expression in salivary gland adenoid cystic carcinoma cells and enhances endothelial cell motility in vitro ... NF-kappaB promotes iNOS and VEGF expression in salivary gland adenoid cystic carcinoma cells and enhances endothelial cell motility in vitro ... In the constitutive and liposaccharide induced condition, NF-kappaB activity was blocked by phosphorylation-defective IkappaBalpha ... Conclusions : Our results suggest that NF-kappaB promotes iNOS and VEGF expression in salivary gland ACC cells and enhances endothelial cell motility in vitro ... Conclusions : Our results suggest that NF-kappaB promotes iNOS and VEGF expression in salivary gland ACC cells and enhances endothelial cell motility in vitro
Szczepanski et al., Cancer Res 2009 (Carcinoma, Squamous Cell...) : LPS binding to TLR4 on tumor cells enhanced proliferation, activated phosphatidylinositol 3-kinase/Akt pathway, up-regulated IRAK-4 expression, induced nuclear NF-kappaB translocation, and increased production ( P < 0.05 ) of interleukin (IL)-6, IL-8, vascular endothelial growth factor , and granulocyte macrophage colony stimulating factor ... LPS binding to TLR4 on tumor cells enhanced proliferation, activated phosphatidylinositol 3-kinase/Akt pathway, up-regulated IRAK-4 expression, induced nuclear NF-kappaB translocation, and increased production ( P < 0.05 ) of interleukin (IL)-6, IL-8, vascular endothelial growth factor, and granulocyte macrophage colony stimulating factor ... LPS binding to TLR4 on tumor cells enhanced proliferation, activated phosphatidylinositol 3-kinase/Akt pathway, up-regulated IRAK-4 expression, induced nuclear NF-kappaB translocation, and increased production ( P < 0.05 ) of interleukin (IL)-6 , IL-8, vascular endothelial growth factor, and granulocyte macrophage colony stimulating factor ... LPS binding to TLR4 on tumor cells enhanced proliferation, activated phosphatidylinositol 3-kinase/Akt pathway, up-regulated IRAK-4 expression, induced nuclear NF-kappaB translocation, and increased production ( P < 0.05 ) of interleukin (IL)-6, IL-8 , vascular endothelial growth factor, and granulocyte macrophage colony stimulating factor ... LPS binding to TLR4 on tumor cells enhanced proliferation, activated phosphatidylinositol 3-kinase/Akt pathway, up-regulated IRAK-4 expression, induced nuclear NF-kappaB translocation, and increased production ( P < 0.05 ) of interleukin (IL)-6, IL-8, vascular endothelial growth factor, and granulocyte macrophage colony stimulating factor
Kuo et al., J Agric Food Chem 2009 (Bone Neoplasms...) : The results showed that 5GG suppressed the EGF induced NF-kappaB nuclear translocation and also abrogated the EGF induced activation of c-jun N-terminal kinase ( JNK ), an upstream modulator of NF-kappaB
Castier et al., Antioxid Redox Signal 2009 : Hence, induction of NF-kappaB by shear stress contributes to MMP induction and allows long-term flow induced vascular enlargement
Kim et al., J Nutr 2009 : Therefore, we examined the effects of curcumin ( Cur ) and limonin ( Lim ) feeding on NF-kappaB dependent CD4 ( + ) T-cell proliferation
Kim et al., Carcinogenesis 2009 (Uterine Cervical Neoplasms) : Involvement of NF-kappaB and AP-1 in COX-2 upregulation by human papillomavirus 16 E5 oncoprotein ... NF-kappaB inhibition blocked COX-2 expression more potently than inhibition of AP-1
McGrath et al., Arterioscler Thromb Vasc Biol 2009 (Arteritis...) : Silencing DHCR24 expression increased NF-kappaB ( 1.2-fold, P < 0.05 ), VCAM-1 ( 30-fold, P < 0.05 ), and NF-kappaB p50 ( 4-fold, P < 0.05 ) and p65 subunits ( 150-fold, P < 0.05 ) ... TNF-alpha activation of siDHCR24 treated cells increased expression of VCAM-1 ( 550-fold, P < 0.001 ) and NF-kappaB ( 9-fold, P < 0.001 ) that could no longer be suppressed by rHDLs
Xia et al., International journal of molecular sciences 2008 : Silencing RAGE expression by specific siRNA effectively suppressed NF-kappaB activity, hepatic stellate cell activation, and accumulation of extracellular matrix proteins in the fibrotic liver, and also greatly improved the histopathology and the ultrastructure of liver cells
Uhl et al., Viral Immunol 2009 (Asthma...) : To investigate the underlying mechanism ( s ) for the increased TNF-alpha expression, the role of nuclear factor-kappaB (NF-kappaB) , an important regulator of TNF-alpha gene transcription, was examined
Kahlon et al., Bioorg Med Chem 2009 : The structure-activity relationship of various substituents on an imidazoline core structure was evaluated for the ability to inhibit NF-kappaB mediated IL-6 production ... Several derivatives were found to inhibit NF-kappaB mediated IL-6 production in the nanomolar range in IL-1beta stimulated human blood
Myung et al., J Pharmacol Exp Ther 2009 : In accordance, NFkappaB dependent cellular FLICE-like inhibitory protein expression level was found to be reduced by 17AAG ... These results demonstrate that the Hsp90 inhibitor induces HSC apoptosis via a sphingomyelinase- and NFkappaB dependent mechanism
Binder et al., Nat Med 2009 (Bone Resorption...) : In normal mice, activation of CCR2 in osteoclast progenitor cells results in both nuclear factor-kappaB (NF-kappaB) and extracellular signal related kinase 1 and 2 ( ERK1/2 ) signaling but not that of p38 mitogen activated protein kinase or c-Jun N-terminal kinase
Park et al., Anticancer Res 2009 (Fibrosarcoma) : As the induction of ICAM-1 requires activation of the transcription factor NF-kappaB , the effects of resveratrol on the activation of this factor in HT1080 cells was also investigated
Giri et al., Anticancer Res 2009 (Colonic Neoplasms) : There was also a marked reduction of cyclin dependent kinase (CDK)2, CDK4 and cyclin B expression and up-regulation of CDK inhibitors ( p21, p27 ) and p53, accompanied by attenuation of Akt signaling and nuclear factor-kappa B (NF-kappaB) activation
Guo et al., BMC cancer 2009 (Carcinoma, Hepatocellular...) : Altogether, these findings revealed a possible effect of HSP27 on apoptosis in metastatic HCC cells, in which HSP27 may regulate NF-kB pathway activation
Kim et al., BMB Rep 2009 : In contrast, another NF-kappaB inhibitor, BMS-345541, did not activate the MAP kinase pathways or induce expression of c-Fos
Thomas et al., Cancer Res 2009 : Tumor necrosis factor (TNF) receptor associated factor 2 ( TRAF2 ) is an adaptor protein that modulates the activation of the c-Jun NH ( 2 ) terminal kinase ( JNK ) /c-Jun and IkappaB kinase (IKK)/nuclear factor-kappaB (NF-kappaB) signaling cascades in response to TNFalpha stimulation ... Importantly, constitutive TRAF2 phosphorylation increased both basal and inducible NF-kappaB activation and rendered Ha-Ras-V12 transformed cells resistant to stress induced apoptosis
Zhang et al., Biol Pharm Bull 2009 : To study the effect of HT on LPS induced NF-kappaB activation in THP-1 cells, Western blot analysis of the nuclear fraction of cell lysates was performed ... The antioxidant N-acetylcysteine (NAC) also showed the same effects as HT on LPS induced ROS and NO generation, change of GSH level, and NF-kappaB activation
Okayama et al., Int J Hyperthermia 2009 (Colonic Neoplasms...) : The GGA plus hyperthermia pretreatment group also showed a lower number of fixed cells in lungs and lower activation of NF-kappa-B by TNF-alpha than the other cell groups
Ottonello et al., Br J Pharmacol 2009 : The activity of oxaprozin was related to inhibition of Akt activation that, in turn, prevented p38 MAPK, IKK and NF-kappaB activation ... Consistently, the inhibition of NF-kappaB activation reduced the production of the anti-apoptotic molecule XIAP , leading to uncontrolled activity of caspase 3
Sponza et al., Cell Immunol 2009 (Inflammation) : We have previously shown that IFI16 is required for proinflammatory gene stimulation by IFN-gamma through the NF-kappaB complex ... We have previously shown that IFI16 is required for proinflammatory gene stimulation by IFN-gamma through the NF-kappaB complex
Bhui et al., Cancer Lett 2009 (Skin Neoplasms) : Bromelain inhibits COX-2 expression by blocking the activation of MAPK regulated NF-kappa B against skin tumor-initiation triggering mitochondrial death pathway
Sudhakar et al., PloS one 2009 : NF-kappaB mediates tumor necrosis factor alpha induced expression of optineurin, a negative regulator of NF-kappaB ... NF-kappaB mediates tumor necrosis factor alpha induced expression of optineurin , a negative regulator of NF-kappaB ... Blocking of NF-kappaB activation resulted in inhibition of TNFalpha induced optineurin gene expression ... Overexpressed optineurin partly inhibited TNFalpha induced NF-kappaB activation in Hela cells ... Overexpressed optineurin partly inhibited TNFalpha induced NF-kappaB activation in Hela cells ... Downregulation of optineurin by shRNA resulted in an increase in TNFalpha induced as well as basal NF-kappaB activity ... In addition these results suggest that there is a negative feedback loop in which TNFalpha induced NF-kappaB activity mediates expression of optineurin, which itself functions as a negative regulator of NF-kappaB
Liu et al., J Immunol 2009 (Acute Lung Injury) : These data demonstrate that p53 regulates NF-kappaB activity in inflammatory cells and suggest that modulation of p53 may have potential therapeutic benefits in acute inflammatory conditions, such as ALI
Ahmad et al., J Immunol 2009 : In addition, IL-1beta induced phosphorylation of downstream effectors, IkappaB kinase alphabeta, IkappaBalpha, and activation of transcription factor NF-kappaB was significantly reduced in the MyD88-, IRAK1-, TRAF6-, or Ras-deficient cells
Raymond et al., PLoS Pathog 2009 (Pneumonia) : We demonstrate that although NF-kappaB is essential for IL-8 expression, LT downregulates this expression without interfering with NF-kappaB activation in epithelial cells
Ebihara et al., Exp Eye Res 2009 : TWEAK induced phosphorylation of NF-kappaB and MAP kinase in corneal myofibroblasts
Madge et al., Methods Mol Biol 2009 : We describe in this chapter three separate methods to determine the effects of this NEMO binding domain (NBD) peptide on pro-inflammatory NF-kappaB signaling in response to tumor necrosis factor (TNF)
Yamaura et al., Lasers Surg Med 2009 (Arthritis, Rheumatoid) : TNF-alpha induced activation of NF-kappaB is not altered by 810 nm radiation using 25 J/cm ( 2 )
Liu et al., Zhong Nan Da Xue Xue Bao Yi Xue Ban 2009 (Acute Lung Injury...) : Hemin further upregulated the expression of HO-1 mRNA, decreased NF-kappaB activity drastically, and inhibited the serum levels of TNF-alpha and IL-6 significantly ( P < 0.05 ) ... Hemin further upregulated the expression of HO-1 mRNA, decreased NF-kappaB activity drastically, and inhibited the serum levels of TNF-alpha and IL-6 significantly ( P < 0.05 )
Gionet et al., J Cell Biochem 2009 (Breast Neoplasms) : Ectopic expression of the ERalpha reduced cell numbers in ER+ and ER- breast cancer cell lines while NF-kappaB binding activity and the expression of several NF-kappaB regulated proteins were reduced in ER- cells
Cohen et al., Cancer Res 2009 (Carcinoma, Squamous Cell...) : However, in HNSCC cells that retained residual TGFbeta signaling, TGFbeta1 inhibited both constitutive and tumor necrosis factor alpha stimulated NF-kappaB activity
Xue et al., Physiol Genomics 2009 : NF-kappaB regulates thrombin induced ICAM-1 gene expression in cooperation with NFAT by binding to the intronic NF-kappaB site in the ICAM-1 gene ... Activation of NF-kappaB is essential for protease activated receptor-1 (PAR-1) mediated ICAM-1 expression in endothelial cells
Krejsgaard et al., Blood 2009 (Lymphoma, T-Cell, Cutaneous...) : Here, we demonstrate nuclear factor-kappa B (NF-kappaB) mediated ectopic expression of Blk in malignant T-cell lines established from patients with cutaneous T-cell lymphoma ( CTCL )
Kim et al., J Pharmacol Sci 2009 : As a mechanism of action, MSA inhibited LPS induced activation of NF-kappaB , ERK, p38, and JNK, which played pivotal roles in toll-like receptor 4-mediated DC maturation
Boost et al., Int J Mol Med 2009 : We, therefore, aimed to investigate the effects of the volatile anesthetics sevoflurane and isoflurane on NF-kappaB/IkappaBalpha dependent intracellular signal transduction in human monocytic THP-1 cells induced by tumor necrosis factor-alpha (TNF-alpha) and production of interleukin-8 (IL-8) and downstream heme oxygenase-1 (HO-1) ... We, therefore, aimed to investigate the effects of the volatile anesthetics sevoflurane and isoflurane on NF-kappaB/IkappaBalpha dependent intracellular signal transduction in human monocytic THP-1 cells induced by tumor necrosis factor-alpha (TNF-alpha) and production of interleukin-8 (IL-8) and downstream heme oxygenase-1 (HO-1)
Myskiw et al., J Virol 2009 : PKR dependent activation of p38 and NF-kappaB was required for vaccinia virus induced INHBA expression, whereas induction of TNF-alpha required only PKR dependent NF-kappaB activation ... PKR dependent activation of p38 and NF-kappaB was required for vaccinia virus induced INHBA expression, whereas induction of TNF-alpha required only PKR dependent NF-kappaB activation ... PKR dependent activation of p38 and NF-kappaB was required for vaccinia virus induced INHBA expression, whereas induction of TNF-alpha required only PKR dependent NF-kappaB activation ... IL-6 induction is regulated by NF-kappaB, while IFN-beta induction is mediated by IFN-beta promoter stimulator 1 and IFN regulatory factor 3/NF-kappaB ... IL-6 induction is regulated by NF-kappaB , while IFN-beta induction is mediated by IFN-beta promoter stimulator 1 and IFN regulatory factor 3/NF-kappaB
Hu et al., Am J Physiol Cell Physiol 2009 : PD-98059 blocked IL-1beta induced phosphorylation of IKK2, degradation of IkappaB-alpha, and phosphorylation and nuclear translocation of NF-kappaB subunit p65, whereas SB-203580 had a marginal effect, implying that the effect of ERK1/2 is exerted on the canonical IKK2/IkappaB-alpha/p65 pathway of NF-kappaB activation but that the effect of p38 MAPK may not predominantly involve NF-kappaB signaling ... PD-98059 blocked IL-1beta induced phosphorylation of IKK2, degradation of IkappaB-alpha, and phosphorylation and nuclear translocation of NF-kappaB subunit p65, whereas SB-203580 had a marginal effect, implying that the effect of ERK1/2 is exerted on the canonical IKK2/IkappaB-alpha/p65 pathway of NF-kappaB activation but that the effect of p38 MAPK may not predominantly involve NF-kappaB signaling
Sutcliffe et al., Br J Pharmacol 2009 : Transcriptional regulation of monocyte chemotactic protein-1 release by endothelin-1 in human airway smooth muscle cells involves NF-kappaB and AP-1
Qi et al., Mol Immunol 2009 : Moreover, inhibition of NF-kappaB suppressed TARC and MDC production induced by IFN-gamma plus TNF-alpha
Suliburk et al., Am J Physiol Gastrointest Liver Physiol 2009 (Drug-Induced Liver Injury...) : Although CO abolished the ability of LPS to diminish PPAR-gamma activity, it enhanced NF-kappaB activity
Tanaka et al., Mol Cancer Res 2009 (Colonic Neoplasms...) : PRMT5 contributed to TRAIL induced activation of inhibitor of kappaB kinase (IKK) and nuclear factor-kappaB (NF-kappaB) , leading to induction of several NF-kappaB target genes ... PRMT5 contributed to TRAIL induced activation of inhibitor of kappaB kinase (IKK) and nuclear factor-kappaB (NF-kappaB) , leading to induction of several NF-kappaB target genes ... PRMT5 had no effect on TNF-alpha mediated NF-kappaB signaling ... PRMT5 had no effect on TNF-alpha mediated NF-kappaB signaling
Gasparian et al., Cell cycle (Georgetown, Tex.) 2009 : We quantitatively compared the efficacy of various proteasome inhibitors ( MG132, lactacystin and epoxomicin ) and IKK inhibitors ( BAY 11-7082 and PS1145 ) to block NFkappaB activity induced by TNFalpha or TPA and to sensitize LNCaP prostate carcinoma cells to apoptosis
Legarda-Addison et al., Cell Death Differ 2009 : NF-kappaB essential modifier (NEMO)/IkappaB kinase-gamma (IKKgamma)-deficient cells are highly sensitive to apoptosis, and as NEMO is essential for NF-kappaB activation, it has been assumed that this is due to the lack of NF-kappaB
Sun et al., Cell Death Differ 2010 : By deconjugating these K63 linked ubiquitin chains, CYLD negatively regulates NF-kappaB activation, which may contribute to its tumor suppressor function
Li et al., Free Radic Biol Med 2009 (Shock, Septic) : GYY4137 inhibited LPS induced TNF-alpha production in rat blood and reduced the LPS evoked rise in NF-kappaB activation, inducible nitric oxide synthase/cyclooxygenase-2 expression, and generation of PGE ( 2 ) and nitrate/nitrite in RAW 264.7 macrophages
Zhou et al., Alcohol 2009 : The LPS enhanced in vitro expression of TLR4, TBK1, and production of IFN-gamma and TNF-alpha in isolated intestinal epithelia of the ethanol group were significantly higher than those in either control group ( P < .05 ) and were dramatically inhibited by SST ( P < .05 ), whereas NF-kappaB was activated by LPS only in the control groups
O'Hara et al., Cytokine 2009 : Silencing of Ref-1 in AGS cells inhibited basal and TNF-alpha induced AP-1 and NF-kappaB DNA binding activity, but not their nuclear accumulation
Wu et al., Growth Factors 2008 : CTGF enhanced the mRNA expressions and protein release of fractalkine, MCP-1 and RANTES, and the expressions of phosphorylated ERK1/2, PI3-K and PKB, and activities of NF-KB
Cheng et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2009 : It is concluded that the TNF-alpha induces expressions of IL-8 mRNA, MCP-1 mRNA and NF-kappaB in HUVECs, and NF-kappaB activities signal pathway may play a role in IL-8 mRNA and MCP-1 mRNA expressions
Ikeda et al., FEBS Lett 2009 : BS69 negatively regulates the canonical NF-kappaB activation induced by Epstein-Barr virus derived LMP1 ... In the present study, our manipulation of BS69 expression clearly indicates that BS69 negatively regulates LMP1 mediated NF-kappaB activation and up-regulates IL-6 mRNA expression and IkappaB degradation
Schmitz et al., J Exp Med 2009 (Chromosome Deletion...) : NF-kappaB activation through various stimuli is negatively regulated by the zinc finger protein A20 ... This report identifies TNFAIP3 ( A20 ), a key regulator of NF-kappaB activity, as a novel tumor suppressor gene in cHL and PMBL
Patel et al., J Leukoc Biol 2009 (Disease Susceptibility...) : IL-10 did not influence p-IRAK, IkappaB degradation, or NF-kappaB p65 nuclear translocation in response to MTb, but IL-10 did increase levels of AM BCL-3 , an inhibitor of NF-kappaB nuclear activity
Du et al., Cancer Res 2009 (Neoplasms) : The human inducible nitric oxide synthase ( hiNOS ) gene is regulated by nuclear factor kappaB (NF-kappaB) and has recently been shown to be a target of the Wnt/beta-catenin pathway ... Gel shift for TNFalpha induced hiNOS NF-kappaB activation showed decreased p50 binding and decreased NF-kappaB reporter activity in the beta-catenin-mutant HAbeta18 cells ... NF-kappaB dependent Traf1 protein expression also inversely correlated with the level of beta-catenin ... Furthermore, SW480 cells stably transformed with wild-type adenomatous polyposis coli showed decreased beta-catenin protein and increased TNFalpha induced p65 NF-kappaB binding as well as iNOS and Traf1 expression
Yamaguchi et al., Cell Biol Int 2009 (MAP Kinase Signaling System) : JNK binding protein 1 regulates NF-kappaB activation through TRAF2 and TAK1 ... JNK binding protein 1 regulates NF-kappaB activation through TRAF2 and TAK1 ... JNK binding protein 1 regulates NF-kappaB activation through TRAF2 and TAK1 ... We also found that JNKBP1 could enhance the NF-kappaB activation induced by TAK1 and TRAF2 , and could promote TRAF2 polyubiquitination ... We also found that JNKBP1 could enhance the NF-kappaB activation induced by TAK1 and TRAF2, and could promote TRAF2 polyubiquitination ... We also found that JNKBP1 could enhance the NF-kappaB activation induced by TAK1 and TRAF2, and could promote TRAF2 polyubiquitination
Cho et al., EMBO J 2009 : An acetylation-deficient cyclin T1 mutant dominantly suppresses NF-kappaB mediated activation of the interleukin-8 promoter but continues to synergize normally with the HIV Tat protein to transactivate the HIV long terminal repeat
Olsnes et al., Br J Haematol 2009 (Leukemia, Myeloid, Acute) : Increased DNA binding of NF-kappaB was observed during exposure to PEP005, and the specific NF-kappaB inhibitor BMS-345541 reduced constitutive chemokine release even in the presence of PEP005
Zhang et al., Dev Biol 2009 : Cerberus 's anti-Nodal activity inhibits NF-kappaB activity and decreases relA RNA levels ... Cerberus 's anti-Nodal activity inhibits NF-kappaB activity and decreases relA RNA levels
Chen et al., Arterioscler Thromb Vasc Biol 2009 (Coronary Restenosis...) : Inward remodeling was associated with PECAM-1 dependent NFkappaB activation, surface adhesion molecule expression, and leukocyte infiltration as well as Akt activation and vascular cell proliferation
Gudi et al., J Environ Pathol Toxicol Oncol 2009 : Siva-1 promotes K-48 polyubiquitination of TRAF2 and inhibits TCR mediated activation of NF-kappaB ... Previously, we showed that Siva-1 inhibited the activity of the prosurvival transcription factor NF-kappaB ... The above results demonstrated an important role for endogenous Siva-1 in negatively regulating NF-kappaB activation by targeting TRAF2
Johnston et al., Gene 2009 : These results indicate that canonical NFkappaB signaling is required for TNF induction of the notch ligand jagged-1 in EC ... These results indicate that canonical NFkappaB signaling is required for TNF induction of the notch ligand jagged-1 in EC
Shah et al., Neurosci Lett 2009 : At the molecular level, beta-glucan suppressed TLR mediated NF-kappaB activation, which may be responsible for the diminished capacity of microglia to produce cytokines in response to TLR stimulation
Sheeba et al., J Ethnopharmacol 2009 : Furthermore, Cardiospermum halicacabum L. ethanol extract inhibited the TNF-alpha induced DNA binding activity of NF-kappaB , which was associated with decreased p65 protein level in the nucleus in Jurkat cells
Shan et al., Int Immunopharmacol 2009 : In the further experiments, LPS induced activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK)-c-Jun-activator protein ( AP-1 ) pathway were suppressed significantly by CGA ... In the further experiments, LPS induced activation of nuclear factor-kappaB (NF-kappaB) and c-Jun N-terminal kinase (JNK)-c-Jun-activator protein ( AP-1 ) pathway were suppressed significantly by CGA
Feng et al., Eur J Pharmacol 2009 (Disease Models, Animal...) : Glial activation markers such as macrophage antigen complex-1 ( mac-1 ) and glial fibrillary acidic protein (GFAP), interleukin (IL)-1beta and IL-6, nuclear factor-kappa B (NF-kappaB) activation , and p-p38 mitogen activated protein kinase ( MAPK ) activation in the lumbar spinal cord were determined as well
Precourt et al., Int J Biochem Cell Biol 2009 : In particular, LPS down-regulated PON2 protein expression, which was accompanied with decreased levels of IkappaBalpha , the inhibitor of the proinflammatory transcription factor nuclear factor-kappa B (NF-kappaB)
Dragon et al., Biochem Biophys Res Commun 2009 (Asthma) : Pharmacological inhibition of NF-kappaB did not affect T ( H ) 2-mediated IL-9Ralpha expression in human neutrophils although IFN-gamma and IL-10 down-regulated IL-9Ralpha expression when co-incubated with IL-4, IL-13 or GM-CSF
Yao et al., Dig Liver Dis 2009 (Stomach Neoplasms) : However, TNFalpha induced activation of NF-kappaB was not affected by over activated Notch-1 ... However, TNFalpha induced activation of NF-kappaB was not affected by over activated Notch-1
Broadbelt et al., American journal of physiology. Renal physiology 2009 (Ureteral Obstruction) : This study examined the involvement of epidermal growth factor (EGF) receptor ( EGFR ), nuclear factor-kappaB (NFkappaB) , and signal transducers and activators of transcription 3 ( STAT3 ) in iNOS induction in human proximal tubule ( HKC-8 ) cells in response to pressure or EGF ... These data indicate that EGFR, NFkappaB , and STAT3 are required for human iNOS gene induction in response to pressure or EGF, indicating a similar mechanism of activation
Bumrungpert et al., J Nutr 2009 (Inflammation...) : Furthermore, gamma-MG but not alpha-MG attenuated LPS mediated IkappaB-alpha degradation and nuclear factor-kappaB (NF-kappaB) activity
Cheng et al., Cell Mol Neurobiol 2009 : IL-17 stimulates migration of carotid artery vascular smooth muscle cells in an MMP-9 dependent manner via p38 MAPK and ERK1/2 dependent NF-kappaB and AP-1 activation
Ahmed et al., Arthritis Rheum 2009 (Arthritis, Rheumatoid...) : Inhibition of Mcl-1 by EGCG triggered caspase 3 activity in RA synovial fibroblasts, which was mediated via down-regulation of the TNFalpha induced Akt and NF-kappaB pathways
Tassi et al., Eur J Immunol 2009 : Dectin-1 required PLCgamma2 to activate MAPK, AP-1 and NF-kappaB , which induce cytokine gene expression
Kang et al., Arthritis Res Ther 2009 (Lupus Erythematosus, Systemic...) : Apigenin also causes apoptosis of hyperactive lupus APCs and T and B cells, probably by inhibiting expression of NF-kappaB regulated anti-apoptotic molecules, especially COX-2 and c-FLIP , which are persistently hyperexpressed by lupus immune cells
Chang et al., FASEB J 2009 : The aptamer could effectively antagonize TLR2 , significantly inhibit NF-kappaB activity, and suppress the secretion of the cytokines by > 80 %
Zhang et al., J Mol Biol 2009 : Currently, TNFalpha induced NF-kappaB activation is believed to be impaired in TRAF2 and TRAF5 double knockout ( T2/5 DKO ) cells ... Although TNFalpha induced receptor interacting protein 1 ubiquitination is indeed impaired in T2/5 DKO cells, TNFalpha stimulation further increases IKK activity in these cells, resulting in significantly elevated expression of NF-kappaB target genes to a level higher than that in wild-type cells
Yang et al., Cancer Cell 2009 (Cell Transformation, Neoplastic...) : Wu et al., in this issue of Cancer Cell, show that TNFalpha dependent NFkappaB activation induces COP9 signalosome mediated inhibition of GSK3beta and the SCF(beta-TRCP) ubiquitin ligase, thus leading to stabilization of the transcription factor Snail and promoting cell migration and metastasis ... Wu et al., in this issue of Cancer Cell, show that TNFalpha dependent NFkappaB activation induces COP9 signalosome mediated inhibition of GSK3beta and the SCF(beta-TRCP) ubiquitin ligase, thus leading to stabilization of the transcription factor Snail and promoting cell migration and metastasis
Wu et al., Cancer Cell 2009 (Cell Transformation, Neoplastic...) : We demonstrated that NF-kappaB is required for the induction of COP9 signalosome 2 (CSN2) , which, in turn, blocks the ubiquitination and degradation of Snail
Derudder et al., Nat Immunol 2009 : By genetically ablating IkappaB kinase (IKK) mediated activation of the transcription factor NF-kappaB in the B cell lineage and by analyzing a mouse mutant in which immunoglobulin lambda-chain positive B cells are generated in the absence of rearrangements in the locus encoding immunoglobulin kappa-chain, we define here two distinct, consecutive phases of early B cell development that differ in their dependence on IKK mediated NF-kappaB signaling
Ghosh et al., Translational oncology 2009 : Although further studies are required, we speculate that activation of NFkappaB/cytokine induced neutrophil chemoattractant 2alpha/Cox-2 along with modulation of antioxidant defense mechanisms may create a proinflammatory environment suitable for tumor growth and survival
Hess et al., Bone 2009 : The direct contribution of the NF-kappaB pathway was confirmed in cells that express a constitutively active version of the NF-kappaB inducing kinase IKK2 ( CA-IKK2 ) ... The IKK2/NF-kappaB induced BMP-2 up-regulation results in the enhancement of RUNX2 and Osterix expression, two critical regulators of the osteogenic differentiation program ... We conclude that TNFalpha mediated NF-kappaB activation, although not absolutely required for BMP-2 expression and matrix mineralization nevertheless supports osteogenic differentiation and matrix mineralization by increasing BMP-2 expression
Chang et al., Anticancer Res 2009 (Liver Neoplasms, Experimental) : The luciferase expression by BLI assay reflected that the TPA induced NF-kappaB activity was suppressed by MTX after 16 h treatment ... BLI directly revealed that MTX inhibited cellular transformation by suppressing NF-kappaB activity
Barakat et al., Naunyn Schmiedebergs Arch Pharmacol 2009 (Brain Ischemia...) : Expression of a constitutively active IkappaB kinase (IKK) or the NF-kappaB subunit p65 in neurons stimulated the transcription of cPLA-2 , COX-2, and mPGES-1 ... Expression of a constitutively active IkappaB kinase (IKK) or the NF-kappaB subunit p65 in neurons stimulated the transcription of cPLA-2, COX-2, and mPGES-1 ... Expression of a constitutively active IkappaB kinase (IKK) or the NF-kappaB subunit p65 in neurons stimulated the transcription of cPLA-2, COX-2 , and mPGES-1
Kang et al., Pharmacol Res 2009 : Resveratrol suppressed the expression of TNF-alpha, IL-6, IL-8 and COX-2 through a decrease in the intracellular levels of Ca2+ and ERK 1/2, as well as activation of NF-kappaB ... Resveratrol suppressed the expression of TNF-alpha, IL-6, IL-8 and COX-2 through a decrease in the intracellular levels of Ca2+ and ERK 1/2 , as well as activation of NF-kappaB ... Resveratrol suppressed the expression of TNF-alpha, IL-6, IL-8 and COX-2 through a decrease in the intracellular levels of Ca2+ and ERK 1/2 , as well as activation of NF-kappaB
Kim et al., FASEB J 2009 : Specifically, our data suggest that IkappaBalpha controls the dynamic patterns of nuclear NF-kappaB , while IkappaBepsilon induces cellular heterogeneity of the NF-kappaB activities
Remels et al., Am J Physiol Endocrinol Metab 2009 : The goal of the present study, therefore, was to evaluate whether PPAR activation affects cytokine induced NF-kappaB activity in skeletal muscle ... Using C ( 2 ) C ( 12 ) myotubes as an in vitro model of myofibers, we demonstrate that PPAR, and specifically PPARgamma , activation potently inhibits inflammatory mediator induced NF-kappaB transcriptional activity in a time- and dose dependent manner ... To verify whether muscular NF-kappaB activity in human subjects is suppressed by PPARgamma activation, we examined the effect of 8 wk of rosiglitazone treatment on muscular gene expression of ICAM-1 and IL-8 in type 2 diabetes mellitus patients
Park et al., Carcinogenesis 2009 (Breast Neoplasms...) : Using specific inhibitors, we confirmed that PMA induced cell invasion and MMP-9 expression is primarily regulated by nuclear factor-kappa B (NF-kappaB) activation via phosphatidylinositol 3-kinase (PI3K)/Akt and activator protein-1 (AP-1) activation via extracellular signal regulated kinase ( ERK ) 1/2 ... KPS-A decreased PMA induced transcriptional activation of NF-kappaB and AP-1 and inhibited PMA induced phosphorylation of ERK1/2 and Akt ... KPS-A decreased PMA induced transcriptional activation of NF-kappaB and AP-1 and inhibited PMA induced phosphorylation of ERK1/2 and Akt
Luo et al., Nephrol Dial Transplant 2009 : IL-1 induced the rapid activation of NF-kappaB in PTC ... NF-kappaB activation by IL-1 was transient, with a change from p65/p50 heterodimer to p50/p50 homodimer formation by 24 h and a switch to enhanced Smad signalling response to TGF beta
Cho et al., American journal of physiology. Renal physiology 2009 (Disease Models, Animal...) : The untreated CKD rats exhibited azotemia, hypertension, hypertriglyceridemia, proteinuria, glomerulosclerosis, tubulointerstitial damage, upregulation of MCP-1, plasminogen activator inhibitor-1 ( PAI-1 ), transforming growth factor ( TGF ) -beta , cyclooxygenase (COX)-1, COX-2, and NAD ( P ) H oxidase ( NOX-4, gp91(phox), p47(phox) and p22(phox) subunits ) and activation of NF-kappaB ( IkappaB phosphorylation ) ... The untreated CKD rats exhibited azotemia, hypertension, hypertriglyceridemia, proteinuria, glomerulosclerosis, tubulointerstitial damage, upregulation of MCP-1, plasminogen activator inhibitor-1 ( PAI-1 ), transforming growth factor ( TGF ) -beta, cyclooxygenase (COX)-1, COX-2, and NAD ( P ) H oxidase ( NOX-4, gp91(phox), p47(phox) and p22(phox) subunits ) and activation of NF-kappaB ( IkappaB phosphorylation ) ... The untreated CKD rats exhibited azotemia, hypertension, hypertriglyceridemia, proteinuria, glomerulosclerosis, tubulointerstitial damage, upregulation of MCP-1, plasminogen activator inhibitor-1 ( PAI-1 ), transforming growth factor ( TGF ) -beta, cyclooxygenase (COX)-1 , COX-2, and NAD ( P ) H oxidase ( NOX-4, gp91(phox), p47(phox) and p22(phox) subunits ) and activation of NF-kappaB ( IkappaB phosphorylation ) ... The untreated CKD rats exhibited azotemia, hypertension, hypertriglyceridemia, proteinuria, glomerulosclerosis, tubulointerstitial damage, upregulation of MCP-1, plasminogen activator inhibitor-1 ( PAI-1 ), transforming growth factor ( TGF ) -beta, cyclooxygenase (COX)-1 , COX-2, and NAD ( P ) H oxidase ( NOX-4, gp91(phox), p47(phox) and p22(phox) subunits ) and activation of NF-kappaB ( IkappaB phosphorylation ) ... The untreated CKD rats exhibited azotemia, hypertension, hypertriglyceridemia, proteinuria, glomerulosclerosis, tubulointerstitial damage, upregulation of MCP-1, plasminogen activator inhibitor-1 ( PAI-1 ), transforming growth factor ( TGF ) -beta, cyclooxygenase (COX)-1, COX-2 , and NAD ( P ) H oxidase ( NOX-4, gp91(phox), p47(phox) and p22(phox) subunits ) and activation of NF-kappaB ( IkappaB phosphorylation )
Wu et al., American journal of physiology. Renal physiology 2009 (Nephritis) : However, the broad-spectrum caspase inhibitor Boc-d-fmk reduced NF-kB activation as assessed by gel shift assay, reduced phosphorylation of subunit IkappaBalpha, and significantly inhibited TNF induced expression of ICAM-1 and VCAM-1 as assessed by both real-time PCR and flow cytometry ... However, the broad-spectrum caspase inhibitor Boc-d-fmk reduced NF-kB activation as assessed by gel shift assay, reduced phosphorylation of subunit IkappaBalpha, and significantly inhibited TNF induced expression of ICAM-1 and VCAM-1 as assessed by both real-time PCR and flow cytometry ... However, the broad-spectrum caspase inhibitor Boc-d-fmk reduced NF-kB activation as assessed by gel shift assay, reduced phosphorylation of subunit IkappaBalpha, and significantly inhibited TNF induced expression of ICAM-1 and VCAM-1 as assessed by both real-time PCR and flow cytometry ... Specific inhibitors of caspases-8 and -3, but not of caspase-1, reduced TNF induced NF-kappaB activation ... Consistent with this, MLC kinase (MLCK) inhibitor ML-7 reduced TNF induced NF-kappaB activation
Oliver et al., Antioxid Redox Signal 2009 (Anoxia) : Sustained hypoxia enhanced basal and LTalpha1beta2 induced NF-kappaB activity in a manner that was dependent upon the canonical but not the noncanonical signaling pathway ... Knockdown of the p65 subunit of the canonical NF-kappaB pathway was sufficient to abolish the effects of hypoxia on LTalpha1beta2 induced NF-kappaB activity
Song et al., Br J Pharmacol 2009 : Down-regulation of VCAM and E-selectin expression induced by CORM-3 was independent of HO-1 up-regulation and was predominantly due to inhibition of sustained NF-kappaB activation
Kaneda et al., Biomaterials 2009 : Cx43 liposomes containing a soluble NEMO binding domain peptide suppressed the intracellular signaling cascade IL-1beta induced NF-kappaB activation and cyclooxygenase-2 expression in Cx43 expressing cells, confirming effective peptide transfer into the cell
Lee et al., Biochem Pharmacol 2009 : Luteolin attenuated ligand independent activation of IRF3 or NFkappaB induced by TLR4, TRIF, or TBK1 , while it did not inhibit TLR oligomerization ... Luteolin attenuated ligand independent activation of IRF3 or NFkappaB induced by TLR4 , TRIF, or TBK1, while it did not inhibit TLR oligomerization
Surazynski et al., Pharmacol Res 2009 (Inflammation) : The mechanism of IL-1 dependent stimulation of NO production was found at the level of iNOS expression and activation of NF-kappaB
Cho et al., Immunol Lett 2009 : NF-kappaB inhibition leads to increased synthesis and secretion of MIF in human CD4+ T cells ... To examine the effects of nuclear factor kappa B (NF-kappaB) inhibition on the secretion of macrophage migration inhibitory factor ( MIF ) in human CD4 ( + ) T cells ... These results indicate that pharmacological inhibition of NF-kappaB causes the release of MIF through de novo synthesis of MIF and the secretion of preformed MIF in CD4 ( + ) T cells through the production of reactive oxygen species
Ma et al., Toxicol Lett 2009 (Lung Diseases) : In the present study, we investigated the cytotoxic effect of BLM on cultured human bronchial epithelial cells ( BECs ) and first confirmed that BLM induced the transcriptional activation of NF-kappaB signaling in BECs ... GSK3beta is known to be a key downstream target of Akt, and LY294002, the PI3K ( phosphatidylinositol 3-kinase ) /Akt inhibitor, which promoted the dephosphorylation of GSK3beta, significantly attenuated BLM induced NF-kappaB activation ... Next, we further observed that constitutively active GSK3beta stabilized the inhibitor of NF-kappaB ( IkappaBalpha ), inhibited p65 nuclear translocation and partially blocked BLM induced NF-kappaB activation ... Next, we further observed that constitutively active GSK3beta stabilized the inhibitor of NF-kappaB ( IkappaBalpha ), inhibited p65 nuclear translocation and partially blocked BLM induced NF-kappaB activation ... Next, we further observed that constitutively active GSK3beta stabilized the inhibitor of NF-kappaB ( IkappaBalpha ), inhibited p65 nuclear translocation and partially blocked BLM induced NF-kappaB activation ... These results suggest that BLM can induce the activation of NF-kappaB signaling in BECs and this process is tightly associated with the phosphorylation status of GSK3beta, implying a possible regulatory mechanism of NF-kappaB signaling in BECs during the toxic lung injury induced by BLM
Cheon et al., J Ethnopharmacol 2009 : Production of NO, PGE ( 2 ), TNF-alpha, and IL-1beta by ELISA, mRNA and protein expression of iNOS and COX-2, phosphorylation of MAPKs, and activation of NF-kappaB by RT-PCR and Western blotting were examined in LPS induced RAW 264.7 macrophages ... Production of NO, PGE ( 2 ), TNF-alpha, and IL-1beta by ELISA, mRNA and protein expression of iNOS and COX-2 , phosphorylation of MAPKs, and activation of NF-kappaB by RT-PCR and Western blotting were examined in LPS induced RAW 264.7 macrophages
Lee et al., J Ethnopharmacol 2009 : Moreover, inhibition of LPS induced iNOS expression by BV was dependent on transcriptional activities of AP-1/NF-kappaB through MEK/ERK pathway
Bellail et al., J Cell Mol Med 2010 (Glioblastoma) : Treatment of resistant cells with parthenolide, an inhibitor of inhibitor of kappaB ( I-kappaB ), eliminated TRAIL induced NF-kappaB activity but not TRAIL resistance
Wu et al., Chem Biol Interact 2009 : Therefore, this study shows that propofol at a therapeutic concentration can downregulate TNF-alpha biosynthesis possibly via inhibition of NFkappaB mediated toll-like receptor 4 gene expression
Wang et al., Mol Cell Biol 2009 (Breast Neoplasms...) : RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1
Tang et al., Cell Res 2009 : TRAIL receptor mediates inflammatory cytokine release in an NF-kappaB dependent manner ... The TRADD-TRAF2-NIK-IKKalpha/beta signaling cascade, which plays an essential role in TNF induced NF-kappaB activation , was found to be involved in tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) receptor mediated signal transduction
Melisi et al., Mol Cancer Res 2009 (Neoplasm Metastasis...) : Our results showed that the secretion of IL-1alpha in MiaPaCa-2 cells constitutively activated NF-kappaB and increased the expression of NF-kappaB downstream genes involved in the different steps of the metastatic cascade, such as urokinase-type plasminogen activator, vascular endothelial growth factor, and IL-8
Park et al., Cancer Res 2009 (Brain Neoplasms...) : RIP1 activates PI3K-Akt via a dual mechanism involving NF-kappaB mediated inhibition of the mTOR-S6K-IRS1 negative feedback loop and down-regulation of PTEN ... The death domain kinase receptor interacting protein 1 (RIP1) plays a key role in nuclear factor-kappaB (NF-kappaB) activation and also activates the PI3K-Akt pathway through unknown mechanisms ... However, unlike other signals that regulate mTOR activity without affecting its level, RIP1 negatively regulates mTOR transcription via a NF-kappaB dependent mechanism ... However, unlike other signals that regulate mTOR activity without affecting its level, RIP1 negatively regulates mTOR transcription via a NF-kappaB dependent mechanism
King et al., J Pharmacol Exp Ther 2009 (Carcinoma, Non-Small-Cell Lung...) : Analysis with IkappaBalphaDeltaN, a dominant version of inhibitor of kappaBalpha ( IkappaBalpha ), as well as dominant negative and small-molecule IkappaB kinase (IKK) inhibitors demonstrated that IL-1beta induced TTP is nuclear factor-kappaB (NF-kappaB) dependent
Hideshima et al., Blood 2009 (Multiple Myeloma) : Bortezomib triggered phosphorylation of IkappaB kinase ( IKKbeta ) and its upstream receptor interacting protein 2, whereas IKKbeta inhibitor MLN120B blocked bortezomib induced IkappaBalpha down-regulation and NF-kappaB activation, indicating receptor interacting protein 2/IKKbeta signaling plays crucial role in bortezomib induced NF-kappaB activation ... Bortezomib triggered phosphorylation of IkappaB kinase ( IKKbeta ) and its upstream receptor interacting protein 2, whereas IKKbeta inhibitor MLN120B blocked bortezomib induced IkappaBalpha down-regulation and NF-kappaB activation, indicating receptor interacting protein 2/IKKbeta signaling plays crucial role in bortezomib induced NF-kappaB activation
Killeen et al., Br J Cancer 2009 (Carcinoma...) : LPS activates NF-kappaB through TLR-4
Maitra et al., Journal of inflammation (London, England) 2009 : Activation of the transcription factor NFkappaB through Toll-like receptors ( TLR ) following bacterial infection is principally involved in regulating lung inflammation in CF. NFkappaB regulates the transcription of several genes that are involved in inflammation, anti-apoptosis and anti-microbial activity, and hyper-activation of this transcription factor leads to a potent inflammatory response ... Our data demonstrate that WFA blocks PAF induced activation of NFkappaB as determined using reporter assays, IL-8 measurements and high-content fluorescent imaging of NFkappaB subunit p65 translocation
Wang et al., Int Immunopharmacol 2009 : Nuclear factor-kappaB (NF-kappaB) could regulate transcription of VCAM-1 and ICAM-1 genes ... Nuclear factor-kappaB (NF-kappaB) could regulate transcription of VCAM-1 and ICAM-1 genes
Zorde-Khvalevsky et al., Hepatology 2009 : Furthermore, within the first hours, we observed a dramatic TLR3 dependent NF-kappaB activation and an increase in Rip3 levels in hepatocytes, accompanied by caspase-8 activation but without an apoptotic outcome
Xiao et al., Clin Immunol 2009 (Glomerulonephritis, IGA) : IgA-HMC conditioned medium up-regulated expression of ICAM-1, IL-6 and ATR1 and activated NF-kappaB and ERK1/2 in PTEC
Wang et al., Cell Death Differ 2009 : In this study, we found that p53 upregulated modulator of apoptosis ( PUMA ), a p53 downstream target and a BH3-only Bcl-2 family member, is directly regulated by NF-kappaB in response to TNF-alpha ... In this study, we found that p53 upregulated modulator of apoptosis ( PUMA ), a p53 downstream target and a BH3-only Bcl-2 family member, is directly regulated by NF-kappaB in response to TNF-alpha
Gavriil et al., Mol Carcinog 2009 (Melanoma...) : Specific IKKbeta inhibitor IV blocks Streptonigrin induced NF-kappaB activity and potentiates its cytotoxic effect on cancer cells
Rasheed et al., Arthritis Res Ther 2009 : In addition, EGCG inhibited the phosphorylating activity of IKKbeta kinase in an in vitro activity assay and EGCG inhibited the AGE mediated activation and DNA binding activity of NF-kappaB by suppressing the degradation of its inhibitory protein IkappaBalpha in the cytoplasm
Batsi et al., Biochem Pharmacol 2009 : Interestingly, NF-kappaB activity and p50 levels were increased by 2-ME2 and suppression of NF-kappaB signaling reduced p27 ( Kip1 ) expression and sensitized cells to 2-ME2 induced apoptosis ... Interestingly, NF-kappaB activity and p50 levels were increased by 2-ME2 and suppression of NF-kappaB signaling reduced p27 ( Kip1 ) expression and sensitized cells to 2-ME2 induced apoptosis
Thibault et al., Virology 2009 (HIV Infections) : Here we demonstrate that TLR5 stimulation induces activation of NF-kappaB and reactivate latent HIV-1 in CD4+ T lymphoid cells
Xiu-li et al., Mol Immunol 2009 (Endometriosis) : Pretreatment the PMs with ERB-041 resulted in a significant inhibition of LPS induced iNOS expression and NF-kappaB activation by preventing its nuclear translocation ... Pretreatment the PMs with ERB-041 resulted in a significant inhibition of LPS induced iNOS expression and NF-kappaB activation by preventing its nuclear translocation
Yu et al., Nutr Metab Cardiovasc Dis 2010 : In this study, we investigated the effects of curcumin on tumor necrosis factor-alpha (TNF-alpha) induced cell migration, the formation of intracellular reactive oxygen species ( ROS ), the translocation of nuclear factor-kappaB (NFkappaB) and the activation and expression of MMP-9 in human aortic smooth muscle cells ( HASMCs ) ... Furthermore, the production of ROS and the nuclear translocation of NF-kappaB p50 and p65 induced by TNF-alpha were dose-dependently suppressed by curcumin pretreatment
Migita et al., Rheumatology (Oxford) 2009 (Arthritis, Rheumatoid) : SAA induced CCL20 production was down-regulated by NF-kappaB inhibition and partially by c-jun N-terminal kinase ( JNK ) inhibition
Zhang et al., Clin Cancer Res 2009 (Neoplasm Recurrence, Local...) : In prostate cancer cell lines, TGF-beta induced EMT was mediated by NF-kappaB signaling ... The detection of NF-kappaB mediated TGF-beta induced EMT in primary tumors predicts disease recurrence in prostate cancer patients following radical prostatectomy
Chang et al., Nat Med 2009 (Osteoporosis) : Inhibition of IKK-NF-kappaB enhances the expression of Fos related antigen-1 (Fra-1) , an essential transcription factor involved in bone matrix formation in vitro and in vivo
Kashima et al., Oncogene 2009 (Neoplasms...) : Expression of CHFR inhibited NF-kappaB reporter activity, whereas knockdown of CHFR activated reporter activity
Pan et al., Braz J Med Biol Res 2009 (Inflammation) : Ang II stimulation also promoted the activation of nuclear factor-kappa B (NF-kappaB) ... These data suggest that the inhibition of NAD ( P ) H oxidase dependent NF-kappaB signaling reduces the increase in MCP-1 production by GECs induced by Ang II ... These data suggest that the inhibition of NAD ( P ) H oxidase dependent NF-kappaB signaling reduces the increase in MCP-1 production by GECs induced by Ang II
Pena et al., Infect Immun 2009 : P. aeruginosa strain PAK DeltafliC ( flagellin knockout ) did not activate NF-kappaB or interleukin-8 (IL-8) but inhibited flagellin activated NF-kappaB by 40 to 50 % and IL-8 secretion by 20 to 25 % ... PAK DeltafliC also inhibited NF-kappaB induced by IL-1beta and Toll-like receptor 2 agonist Pam3CSK4 ... PAK DeltafliC also inhibited NF-kappaB induced by IL-1beta and Toll-like receptor 2 agonist Pam3CSK4
Li et al., Med Oncol 2010 (Multiple Myeloma) : Making use of the rhTACI-Fc to block the BAFF- and APRIL induced activation of NF-kappaB2 , we demonstrated that the NF-kappaB2-p52/RelB signal contributes to MM cell survival ... Making use of the rhTACI-Fc to block the BAFF- and APRIL induced activation of NF-kappaB2 , we demonstrated that the NF-kappaB2-p52/RelB signal contributes to MM cell survival
Ci et al., Fundam Clin Pharmacol 2009 (Inflammation) : Signal transduction studies showed that avermectin significantly inhibits NF-kappaB p65 translocation into the nucleus and inhibits JNK and p38 phosphorylation protein expression
Zaiss et al., J Immunol 2009 (Adenoviridae Infections) : In direct correlation with the increased viral internalization, antiviral Abs amplified the innate immune response to adenovirus as determined by the expression of NF-kappaB dependent genes, type I IFNs, and caspase dependent IL-1beta maturation
Du et al., J Immunol 2009 : In TNF-alpha treated cells, suppression of MCP-1 by CsA or FK506 was associated with blunted responses of NF-kappaB , the crucial regulator of MCP-1 ... CsA and FK506, as well as other UPR inducers, caused up-regulation of C/EBP family members, especially C/EBPbeta and CHOP ( C/EBP homologous protein ), and overexpression of either C/EBPbeta or CHOP significantly attenuated TNF-alpha triggered NF-kappaB activation ... CsA and FK506, as well as other UPR inducers, caused up-regulation of C/EBP family members, especially C/EBPbeta and CHOP ( C/EBP homologous protein ), and overexpression of either C/EBPbeta or CHOP significantly attenuated TNF-alpha triggered NF-kappaB activation
Park et al., Phytother Res 2010 : Further study demonstrated that glabridin inhibited LPS induced DNA binding activity of NF-kappaB and AP-1 in BV-2 cells
Jiang et al., J Hepatol 2009 : The JAK/STAT- and PI3K/Akt dependent pathways, NF-kappaB activation and expression of the anti-apoptotic proteins Mcl-1 and A1 were evaluated ... The JAK/STAT- and PI3K/Akt dependent pathways, NF-kappaB activation and expression of the anti-apoptotic proteins Mcl-1 and A1 were evaluated
Smith et al., J Neuropathol Exp Neurol 2009 (Disease Models, Animal...) : These data indicate that tumor necrosis factor mediates rapid activation of injury induced NF-kappaB DNA binding in Schwann cells and that these events are associated with inhibition of postinjury axonal sprouting
Prado-Montes de Oca et al., Infect Genet Evol 2009 (Genetic Predisposition to Disease...) : SNP 668C probably abrogates NF-kappaB dependent DEFB1 upregulation leading to L-lep variant
Zucchelli et al., Inflamm Bowel Dis 2009 (Colitis, Ulcerative...) : An in vitro system was set up to evaluate the potential impact of SLC15A1 polymorphism on PepT1 transporter function by quantification of NOD2 mediated activation of NF-kappaB
Lee et al., Int Immunopharmacol 2009 (Dermatitis, Atopic) : Hirsutenone, dexamethasone, cyclosporin A and Bay 11-7085 inhibited the TNF-alpha induced phosphorylation of inhibitory kappaB and the activation of nuclear factor (NF)-kappaB
Verstrepen et al., Biochem Pharmacol 2009 : Overexpression of ABINs inhibits NF-kappaB activation by tumor necrosis factor (TNF) and several other stimuli ... Similar to A20, ABIN-1 and ABIN-3 expression is NF-kappaB dependent , implicating a potential role for the A20/ABIN complex in the negative feedback regulation of NF-kappaB activation ... Similar to A20, ABIN-1 and ABIN-3 expression is NF-kappaB dependent , implicating a potential role for the A20/ABIN complex in the negative feedback regulation of NF-kappaB activation
Sun et al., Cell Signal 2009 : MEKK3 is required for lysophosphatidic acid induced NF-kappaB activation ... Although LPA induces NF-kappaB activation by acting on its GPCR receptor, the molecular mechanism of LPA receptor mediated NF-kappaB activation remains to be well defined ... In the present study, by using MEKK3-, TAK1-, and IKKbeta-deficient murine embryonic fibroblasts ( MEFs ), we found that MEKK3 but not TAK1 deficiency impairs LPA and protein kinase C ( PKC ) -induced IkappaB kinase (IKK)-NF-kappaB activation, and IKKbeta is required for PKC induced NF-kappaB activation ... Together, our results provide the genetic evidence that MEKK3 but not TAK1 is required for LPA receptor mediated IKK-NF-kappaB activation ... Together, our results provide the genetic evidence that MEKK3 but not TAK1 is required for LPA receptor mediated IKK-NF-kappaB activation
Seidel et al., Am J Physiol Lung Cell Mol Physiol 2009 (Chronic Disease...) : DMF inhibited TNFalpha induced NF-kappaB p65 phosphorylation, NF-kappaB nuclear entry, and NF-kappaB-DNA complex formation, whereas PDGF-BB appeared not to activate NF-kappaB within 60 min
Hoffmann et al., Gut 2009 (Disease Models, Animal...) : D-NPs were selectively taken up by Kupffer cells and inhibited NF-kappaB activation
Kim et al., Arch Pharm Res 2009 (Colonic Neoplasms) : NF-kappaB target gene expression of apoptotic cell death proteins ( Bax, caspase-3, caspase-9 ) was significantly enhanced, but the expression of anti-apoptotic genes and cell proliferation marker genes ( Bcl-2, inhibitor of apoptosis protein ( IAP-1 ) and X chromosome IAP (XIAP), Cox-2, c-Fos, c-Jun and cyclin D1 ) was significantly inhibited by the combined treatment compared to Rg3 or docetaxel alone ... NF-kappaB target gene expression of apoptotic cell death proteins ( Bax, caspase-3, caspase-9 ) was significantly enhanced, but the expression of anti-apoptotic genes and cell proliferation marker genes ( Bcl-2, inhibitor of apoptosis protein ( IAP-1 ) and X chromosome IAP (XIAP), Cox-2, c-Fos , c-Jun and cyclin D1 ) was significantly inhibited by the combined treatment compared to Rg3 or docetaxel alone ... NF-kappaB target gene expression of apoptotic cell death proteins ( Bax, caspase-3, caspase-9 ) was significantly enhanced, but the expression of anti-apoptotic genes and cell proliferation marker genes ( Bcl-2, inhibitor of apoptosis protein ( IAP-1 ) and X chromosome IAP (XIAP) , Cox-2, c-Fos, c-Jun and cyclin D1 ) was significantly inhibited by the combined treatment compared to Rg3 or docetaxel alone ... NF-kappaB target gene expression of apoptotic cell death proteins ( Bax, caspase-3, caspase-9 ) was significantly enhanced, but the expression of anti-apoptotic genes and cell proliferation marker genes ( Bcl-2, inhibitor of apoptosis protein ( IAP-1 ) and X chromosome IAP (XIAP), Cox-2, c-Fos, c-Jun and cyclin D1 ) was significantly inhibited by the combined treatment compared to Rg3 or docetaxel alone ... NF-kappaB target gene expression of apoptotic cell death proteins ( Bax, caspase-3, caspase-9 ) was significantly enhanced, but the expression of anti-apoptotic genes and cell proliferation marker genes ( Bcl-2, inhibitor of apoptosis protein ( IAP-1 ) and X chromosome IAP (XIAP), Cox-2 , c-Fos, c-Jun and cyclin D1 ) was significantly inhibited by the combined treatment compared to Rg3 or docetaxel alone ... NF-kappaB target gene expression of apoptotic cell death proteins ( Bax, caspase-3, caspase-9 ) was significantly enhanced, but the expression of anti-apoptotic genes and cell proliferation marker genes ( Bcl-2 , inhibitor of apoptosis protein ( IAP-1 ) and X chromosome IAP (XIAP), Cox-2, c-Fos, c-Jun and cyclin D1 ) was significantly inhibited by the combined treatment compared to Rg3 or docetaxel alone
Qi et al., J Cell Physiol 2009 : JAKs inhibitors suppressed IFN-gamma plus TNF-alpha induced production of CXCL10 in parallel with activation of STAT1 and NF-kappaB, while ERK inhibitor suppressed production of CXCL10 as well as activation of NF-kappaB , but not that of STAT1 ... JAKs inhibitors suppressed IFN-gamma plus TNF-alpha induced production of CXCL10 in parallel with activation of STAT1 and NF-kappaB, while ERK inhibitor suppressed production of CXCL10 as well as activation of NF-kappaB , but not that of STAT1 ... Of note, TNF-alpha induced NF-kappaB may be the primary pathway contributing to CXCL10 production in THP-1 cells ... IFN-gamma potentiates TNF-alpha induced CXCL10 production in THP-1 cells by increasing the activation of STAT1 and NF-kappaB through JAK1 and JAK2 ... IFN-gamma potentiates TNF-alpha induced CXCL10 production in THP-1 cells by increasing the activation of STAT1 and NF-kappaB through JAK1 and JAK2
Jie Zhao et al., Innate Immun 2009 (Aspergillosis...) : In addition, the A. fumigatus antigens induced production of IL-1beta and IL-10 in supernatants of corneal epithelial cells was also attenuated by NF-kappaB inhibitor ... In addition, the A. fumigatus antigens induced production of IL-1beta and IL-10 in supernatants of corneal epithelial cells was also attenuated by NF-kappaB inhibitor ... These findings indicate that the cornea has functional TLR2 and TLR4, and activation of TLR2 and TLR4 through NF-kappaB may contribute to pathogenesis of keratomycosis ... These findings indicate that the cornea has functional TLR2 and TLR4, and activation of TLR2 and TLR4 through NF-kappaB may contribute to pathogenesis of keratomycosis
Chan et al., Cancer Cell 2009 (Neoplasms, Experimental...) : These two factors are regulated by PHD2 in a HIF independent but NF-kappaB dependent manner
Ramanathan et al., J Leukoc Biol 2009 : A ( 2A ) R expression in unstimulated macrophages was low but was induced rapidly by LPS in a NF-kappaB dependent manner
Lee et al., Exp Mol Med 2009 : Tat-SOD inhibited LPS induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases ... Tat-SOD inhibited LPS induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases ... Tat-SOD inhibited LPS induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases
Filer et al., Arthritis Rheum 2009 (Arthritis, Rheumatoid) : NF-kappaB activation was required for production of both IL-6 and CCL5 ... NF-kappaB activation was required for production of both IL-6 and CCL5
Hong et al., J Surg Res 2010 : Cyclic AMP inhibits IL-1beta plus IFNgamma induced NF-kappaB translocation in hepatocytes by a PKA independent mechanism ... We previously showed that cAMP inhibits IL-1beta plus IFNgamma induced NF-kappaB binding in primary hepatocytes but the signaling mechanisms responsible for this effect are not understood ... In this study, the role of PKA in mediating the effect of cAMP on NF-kappaB was investigated ... Overexpression of PKA increased IL-1beta plus IFNgamma induced NF-kappaB binding ... Overexpression of PKA increased IL-1beta plus IFNgamma induced NF-kappaB binding ... These results suggest that PKA is not essential for the inhibitory effect of cAMP on NF-kappaB binding activity in hepatocytes ... We demonstrated that cAMP inhibits IL-1beta plus IFNgamma induced NF-kappaB binding due to its blockade of the upstream signal ( s ) leading to IkappaB phosphorylation and degradation, and is mediated by PKA independent signaling pathways
Lin et al., J Endod 2009 : The interaction of NOD2 with downstream signaling molecules ultimately results in the activation of NF-kappaB and production of inflammatory mediators in innate immunity
Minamikawa et al., J Endod 2009 : Furthermore, MTA caused inhibitory kappa B ( IkappaB ) phosphorylation and translocation of nuclear factor-kappa B (NF-kappaB) subunits to nucleus ... Curucumin, an inhibitor of NF-kappaB activation, suppressed MTA induced COX-2 and iNOS mRNA expressions ... Curucumin, an inhibitor of NF-kappaB activation, suppressed MTA induced COX-2 and iNOS mRNA expressions
Fazal et al., J Biol Chem 2009 : Here, we show a central role of cofilin-1, an actin binding protein that promotes actin depolymerization, in linking RhoA/ROCK pathway to dynamic alterations in actin cytoskeleton that are necessary for activation of NF-kappaB and thereby expression of ICAM-1 in these cells ... RNA interference knockdown of cofilin-1 stabilized the actin filaments and inhibited thrombin- and RhoA induced NF-kappaB activity ... Similarly, constitutively inactive mutant of cofilin-1 ( Cof1-S3D ), known to stabilize the actin cytoskeleton, inhibited NF-kappaB activity by thrombin ... Overexpression of wild type cofilin-1 or constitutively active cofilin-1 mutant ( Cof1-S3A ), known to destabilize the actin cytoskeleton, also impaired thrombin induced NF-kappaB activity ... The effect of cofilin-1 depletion on NF-kappaB activity and ICAM-1 expression occurred downstream of IkappaBalpha degradation and was a result of impaired RelA/p65 nuclear translocation and consequently, RelA/p65 binding to DNA ... The effect of cofilin-1 depletion on NF-kappaB activity and ICAM-1 expression occurred downstream of IkappaBalpha degradation and was a result of impaired RelA/p65 nuclear translocation and consequently, RelA/p65 binding to DNA
Kim et al., Biol Pharm Bull 2009 : In addition, gallotannin 15 and 23 ( 0.1-10 microg/ml ) dose-dependently inhibited LPS induced activation of nuclear factor (NF)-kappaB as indicated by inhibition of degradation of I-kappaBalpha, nuclear translocation of NF-kappaB, and NF-kappaB dependent gene reporter assay
Kim et al., Biol Pharm Bull 2009 : Furthermore, fraxinellone significantly attenuated LPS induced DNA binding activity and the transcription activity of nuclear factor-kappa B (NF-kappaB)
Osterlund et al., Clin Exp Allergy 2009 (Asthma) : The release of cytokines and chemokines was regulated by NF-kappaB and MAPK activation in different ways, while expression of ICAM-1 was solely dependent on NF-kappaB activation
Nikolaidis et al., Shock 2010 : Ron receptor tyrosine kinase negatively regulates TNFalpha production in alveolar macrophages by inhibiting NF-kappaB activity and Adam17 production ... To test this hypothesis, we used the wild-type and Ron TK ( -/- ) primary alveolar macrophages and the murine alveolar macrophage cell line, MH-S, to examine the effects of Ron activation on LPS induced TNFalpha production and NF-kappaB activity
Liu et al., Shock 2010 : Furthermore, the inhibition of moscatilin seemed to be dependent on the repression of HIF-1alpha accumulation and NF-kappaB activation
Hosoi et al., Cancer Res 2009 (Neovascularization, Pathologic...) : We further found that NDRG1/Cap43 induced nuclear factor-kappaB (NF-kappaB) signaling attenuation through marked decreases in inhibitor of kappaB kinase (IKK) beta expression and IkappaBalpha phosphorylation ... We further found that NDRG1/Cap43 induced nuclear factor-kappaB (NF-kappaB) signaling attenuation through marked decreases in inhibitor of kappaB kinase (IKK) beta expression and IkappaBalpha phosphorylation ... We further found that NDRG1/Cap43 induced nuclear factor-kappaB (NF-kappaB) signaling attenuation through marked decreases in inhibitor of kappaB kinase (IKK) beta expression and IkappaBalpha phosphorylation
Kim et al., Rheumatology (Oxford) 2009 (Osteoarthritis) : MAP kinases [ p38 , extracellular signal regulated protein kinase ( ERK ) and C-Jun N terminal kinase (JNK) ] and NF-kappaB activation were evaluated by western blotting and by an electrophoretic mobility shift assay, respectively ... MAP kinases [ p38, extracellular signal regulated protein kinase ( ERK ) and C-Jun N terminal kinase (JNK) ] and NF-kappaB activation were evaluated by western blotting and by an electrophoretic mobility shift assay, respectively
Guo et al., J Biol Chem 2009 (Sepsis) : Using an HEK-Blue ( TM ) cell system, we demonstrated that expression of SR-BI suppressed TLR4 mediated NF-kappaB activation ... Using an HEK-Blue ( TM ) cell system, we demonstrated that expression of SR-BI suppressed TLR4 mediated NF-kappaB activation
Tieppo et al., J Nutr 2009 (Liver Cirrhosis...) : Increased NO production, overexpression of iNOS , eNOS, HO-1, and ETB-receptor and activation of NF-kappaB were observed in lung of CBDL rats
Wei et al., Am J Physiol Cell Physiol 2009 : Altogether, the data demonstrate a signaling pathway by which titanium particles induce oxidative stress, stimulate calpain mediated NF-kappaB activation, and activate target gene expression, including COX-2
Yoshimi et al., J Immunol 2009 : Reporter analyses demonstrated that TLR mediated NF-kappaB activation was higher in Trim21 ( -/- ) cells than in wild-type cells, most likely accounting for their enhanced cytokine expression
Hasegawa et al., J Immunol 2009 : Although the role of ASC in caspase-1 mediated IL-1beta and IL-18 maturation is well known, ASC also induces NF-kappaB activation and cytokine gene expression in human cells ... The ASC mediated AP-1 activation was NF-kappaB independent and primarily cell-autonomous response, whereas the STAT3 activation required NF-kappaB activation and was mediated by a factor that can act in a paracrine manner
Hussain et al., J Am Soc Nephrol 2009 (Glomerulonephritis...) : Nephrin deficiency activates NF-kappaB and promotes glomerular injury
Wu et al., Int Immunopharmacol 2009 : The results showed that EM inhibited TNF-alpha induced expressions of RANKL and NF-kappaB at both mRNA and protein levels in a concentration dependent manner
Gupta et al., Am J Physiol Heart Circ Physiol 2009 (Cardiomegaly...) : Myotrophin induced activation of NF-kappaB has been shown to be associated with cardiac hypertrophy ( CH ) that progresses to heart failure ( HF )
Jung et al., Int J Immunopathol Pharmacol 2009 : Apicidin also inhibited LPS induced MAPK activation and NF-kB nuclear translocation in DCs
Min et al., J Ethnopharmacol 2009 (Inflammation) : Inflammatory makers, such as expression of pro-inflammatory cytokines and cyclooxygenase (COX)-2 , and activation of nuclear factor-kappa B (NF-kappaB) , were measured by enzyme linked immunosorbent assays and immunoblot analyses ... Inflammatory makers, such as expression of pro-inflammatory cytokines and cyclooxygenase (COX)-2 , and activation of nuclear factor-kappa B (NF-kappaB) , were measured by enzyme linked immunosorbent assays and immunoblot analyses
Hwang et al., Am J Chin Med 2009 : Furthermore, EGE significantly inhibited TNF-alpha induced intracellular reactive oxygen species ( ROS ) production and p65 NF-kappaB activation by preventing IkappaB-alpha phosphorylation ... Furthermore, EGE significantly inhibited TNF-alpha induced intracellular reactive oxygen species ( ROS ) production and p65 NF-kappaB activation by preventing IkappaB-alpha phosphorylation
Farhana et al., Mol Cancer Ther 2009 : We examined the effect of loss of SHP and Sin3A expression in a number of cell types on 3-Cl-AHPC mediated growth inhibition and apoptosis induction, 3-Cl-AHPC mediated nuclear factor-kappaB (NF-kappaB) activation, and 3-Cl-AHPC mediated increase in c-Fos and c-Jun expression ... 3-Cl-AHPC induction of c-Fos and c-Jun expression as well as NF-kappaB activation was dependent on SHP protein levels ... 3-Cl-AHPC induction of c-Fos and c-Jun expression as well as NF-kappaB activation was dependent on SHP protein levels
Bai et al., Mol Cancer Ther 2009 (Neoplasms) : Conversely, SMC3 induced NF-kappaB activation was found to be mediated by autocrine TNF because this effect of SMC3 was effectively inhibited when TNF was blocked with either a TNF neutralizing antibody or TNF small interfering RNA ... Conversely, SMC3 induced NF-kappaB activation was found to be mediated by autocrine TNF because this effect of SMC3 was effectively inhibited when TNF was blocked with either a TNF neutralizing antibody or TNF small interfering RNA ... In addition, although SMC3 dramatically reduced c-IAP1 level, it had marginal effect on c-IAP2 expression, TNF induced RIP modification, NF-kappaB activation , and downstream antiapoptosis NF-kappaB target expression
Shin et al., Immunopharmacol Immunotoxicol 2009 (Inflammation) : MW inhibited secretion of TNF-alpha , IL-6, and IL-8 possibly by inhibiting NF-kappaB activation ... MW inhibited secretion of TNF-alpha, IL-6 , and IL-8 possibly by inhibiting NF-kappaB activation ... MW inhibited secretion of TNF-alpha, IL-6, and IL-8 possibly by inhibiting NF-kappaB activation
Koelsche et al., Mol Immunol 2009 : In contrast, nuclear factor kappaB (NFkappaB) signaling was not affected by SOCS1 wt or mutants
Samanta et al., Cancer 2009 (Cystadenocarcinoma, Serous...) : The authors have demonstrated that MEKK3 plays a critical role in cytokine mediated NFkappaB activation, and that stable expression of MEKK3 in cultured cells leads to increased NFkappaB activity
Logunov et al., Mol Gen Mikrobiol Virusol 2009 : Various strains of mycoplasmas cause activation of transcriptional factor NF-kB as a result of interaction with different combinations of Toll-like receptors ( TLR )
Binion et al., Am J Physiol Gastrointest Liver Physiol 2009 : Vascular cell adhesion molecule-1 expression in human intestinal microvascular endothelial cells is regulated by PI 3-kinase/Akt/MAPK/NF-kappaB : inhibitory role of curcumin ... Tumor necrosis factor (TNF)-alpha/lipopolysaccharide (LPS) induced VCAM-1 expression in HIMEC was suppressed by Akt small interfering RNA, curcumin, and inhibitors of NF-kappaB ( SN-50 ), p38 MAPK ( SB-203580 ) and PI 3-kinase/Akt ( LY-294002 )
Gao et al., Cell Mol Life Sci 2009 : Transforming growth factor-beta activated kinase 1 ( TAK1 ) is a serine/threonine protein kinase that is critically involved in the activation of NF-kappaB by tumor necrosis factor ( TNFalpha ), interleukin-1beta (IL-1beta) and TLR ligands ... Transforming growth factor-beta activated kinase 1 ( TAK1 ) is a serine/threonine protein kinase that is critically involved in the activation of NF-kappaB by tumor necrosis factor ( TNFalpha ), interleukin-1beta (IL-1beta) and TLR ligands ... Transforming growth factor-beta activated kinase 1 ( TAK1 ) is a serine/threonine protein kinase that is critically involved in the activation of NF-kappaB by tumor necrosis factor ( TNFalpha ), interleukin-1beta (IL-1beta) and TLR ligands ... Transforming growth factor-beta activated kinase 1 ( TAK1 ) is a serine/threonine protein kinase that is critically involved in the activation of NF-kappaB by tumor necrosis factor ( TNFalpha ), interleukin-1beta (IL-1beta) and TLR ligands ... Overexpression of WDR34 inhibited IL-1beta-, polyI : C- and lipopolysaccharide (LPS) induced but not TNFalpha induced NF-kappaB activation, whereas knockdown of WDR34 by a RNA-interference construct potentiated NF-kappaB activation by these ligands
Takahashi-Makise et al., Int J Cancer 2009 (Herpesviridae Infections...) : CEP also inhibited NF-kappaB activation and induced apoptotic cell death in PEL cell lines
Nakatani et al., Arch Oral Biol 2009 : Effects of specific inhibitors of p38 mitogen activated protein kinase ( MAPK ) activity, MAPK kinase activity and NFkB activity on HAoligo induced MMP-1 expression in HPDL cells were also investigated by real-time PCR and Western blot analyses ... Neither inhibition of NFkB nor MAPKK activity affected the MMP-1 expression
Cho et al., Cell 2009 (Necrosis...) : The kinase RIP1 is crucial for programmed necrosis, but also mediates activation of the prosurvival transcription factor NF-kappaB
Dodd et al., FASEB J 2009 (Atrophy) : Hsp27 inhibits IKKbeta induced NF-kappaB activity and skeletal muscle atrophy ... Hsp27 inhibits IKKbeta induced NF-kappaB activity and skeletal muscle atrophy ... Previous work, in nonmuscle cells, has shown that Hsp27 inhibits TNF-alpha induced NF-kappaB activation ... Previous work, in nonmuscle cells, has shown that Hsp27 inhibits TNF-alpha induced NF-kappaB activation ... During skeletal muscle disuse, Hsp25/27 levels are decreased and NF-kappaB activity increased , and this increase in NF-kappaB activity is required for disuse muscle atrophy ... Furthermore, disuse- and IKKbeta induced NF-kappaB transactivation were abolished by Hsp27
Murakami et al., Anticancer Res 2009 : We also investigated tThe inhibitory effects of these compounds on lipopolysaccharide (LPS) stimulated cyclooxygenase-2 (COX-2) mRNA and protein expression and on binding of activator-protein-1 (AP-1) and nuclear factor kappa-B (NF-kappaB) to their respective consensus sequences were also investigated in RAW 264.7 cells ... 2,2'-Biphenol, but not 4,4'-biphenol, showed inhibitory effects on LPS stimulated COX-2 expression and on AP-1 and NF-kappaB binding to their consensus sequences at 1-10 muM
Chen et al., J Biol Chem 2009 : Herein, we examined the role of neutral sphingomyelinase (nSMase)/ceramide in peptidoglycan ( PGN ) -induced NF-kappaB activation and cyclooxygenase-2 (COX-2) expression in macrophages ... Our data demonstrate for the first time that PGN activates the nSMase/ceramide pathway to induce MKK3/6/p38 MAPK activation, which in turn initiates NF-kappaB activation and ultimately induces COX-2 expression in macrophages
Banerjee et al., Cancer Res 2009 (Pancreatic Neoplasms) : Our data provide mechanistic evidence for the first time showing that DIM potentiates the killing of pancreatic cancer cells by down-regulation of constitutive as well as drug induced activation of NF-kappaB and its downstream genes ( Bcl-xL , XIAP, cIAP, and survivin ) ... Our data provide mechanistic evidence for the first time showing that DIM potentiates the killing of pancreatic cancer cells by down-regulation of constitutive as well as drug induced activation of NF-kappaB and its downstream genes ( Bcl-xL, XIAP , cIAP, and survivin )
Cerciat et al., Glia 2010 : Finally, we report that news SERMs, ospemifene and bazedoxifene, exert anti-inflammatory actions by a mechanism involving classical estrogen receptors and by the inhibition of LPS induced NFkappaB p65 transactivation ... Finally, we report that news SERMs, ospemifene and bazedoxifene, exert anti-inflammatory actions by a mechanism involving classical estrogen receptors and by the inhibition of LPS induced NFkappaB p65 transactivation
Cho et al., J Cell Biochem 2009 (Breast Neoplasms...) : KiSS1 suppresses TNFalpha induced breast cancer cell invasion via an inhibition of RhoA mediated NF-kappaB activation ... TNFalpha induced NF-kappaB activation enhances cellular mechanisms including proliferation, migration, and invasion ... However, it is unclear whether KiSS1 regulates TNFalpha induced NF-kappaB activation and further tumor cell migration ... However, it is unclear whether KiSS1 regulates TNFalpha induced NF-kappaB activation and further tumor cell migration ... Both KiSS1 overexpression and KP10 ( kisspeptin-10 ) stimulation inhibited TNFalpha induced NF-kappaB activity, suppressed TNFalpha induced cell migration and cell attachment to fibronectin in breast cancer cells while KP10 has little effect on cancer cell proliferation ... Both KiSS1 overexpression and KP10 ( kisspeptin-10 ) stimulation inhibited TNFalpha induced NF-kappaB activity, suppressed TNFalpha induced cell migration and cell attachment to fibronectin in breast cancer cells while KP10 has little effect on cancer cell proliferation ... Therefore, our data demonstrate that KiSS1 inhibits TNFalpha induced NF-kappaB activation via downregulation of RhoA activation and suppression of breast cancer cell migration and invasion ... Therefore, our data demonstrate that KiSS1 inhibits TNFalpha induced NF-kappaB activation via downregulation of RhoA activation and suppression of breast cancer cell migration and invasion
Dasgupta et al., J Neurosci Res 2009 : A thorough screening of the signaling system confirmed that TNFalpha/IL-1beta stimulation up-regulated nuclear factor kappaB (NFkappaB) signaling in SV-HCECs ... A thorough screening of the signaling system confirmed that TNFalpha/IL-1beta stimulation up-regulated nuclear factor kappaB (NFkappaB) signaling in SV-HCECs ... A thorough screening of the signaling system confirmed that TNFalpha/IL-1beta stimulation up-regulated nuclear factor kappaB (NFkappaB) signaling in SV-HCECs
Song et al., Atherosclerosis 2009 (Coronary Artery Disease) : SAA time-dependently induced transient and significant upregulation of NF-kappaB1 mRNA ; inhibitor studies indicate that activation of NF-kappaB through the ERK1/2 , p38 and JNK MAPKs and the PI3K pathway was involved ... SAA time-dependently induced transient and significant upregulation of NF-kappaB1 mRNA ; inhibitor studies indicate that activation of NF-kappaB through the ERK1/2, p38 and JNK MAPKs and the PI3K pathway was involved
Bielig et al., Mol Immunol 2009 : A function for AAMP in Nod2 mediated NF-kappaB activation
Rehm et al., Int J Cancer 2009 (Lymphoma, Large B-Cell, Diffuse...) : Given the role of NF-kappaB in the transcriptional activation of CCR7 , we identified the involvement of the noncanonical activation pathway in MLBCLs. MLBCLs exhibit a diagnostic chemokine receptor profile that is instrumental in the discrimination from diffuse large B-cell lymphoma not otherwise specified and classical Hodgkin lymphoma
Lin et al., J Agric Food Chem 2009 : FKB blocked the nuclear translocation of NF-kappaB induced by LPS , which was associated with prevention IkappaB degradation, and subsequently decreased NF-kappaB protein levels in the nucleus
Marasco et al., Biochem J 2009 : When tested in cell assays for its capacity to block NF-kappaB activation, this peptide represses activation of NF-kappaB mediated by BCL10, CARMA3 and PMA/ionomycin stimulation ... When tested in cell assays for its capacity to block NF-kappaB activation, this peptide represses activation of NF-kappaB mediated by BCL10 , CARMA3 and PMA/ionomycin stimulation
Oh et al., Gynecol Oncol 2009 (Adenocarcinoma...) : The transcriptional activation of MMP-9 by NF-kappaB was up-regulated in syndecan-1 overexpression ... These findings provide evidence that supports that syndecan-1 may have a critical role in carcinogenic progression, particularly, contributing to the development of proliferative and invasive phenotype through NF-kappaB mediated MMP-9 gene expression in endometrial cancer
Jin et al., Neurosci Res 2009 (Central Nervous System Infections...) : Inhibition of ERK, p38 or PKC decreased the ESP induced activation of NF-kappaB , an important transcription factor for iNOS expression
Bolignano et al., Cancer Lett 2010 (Neoplasm Invasiveness...) : Moreover, in thyroid carcinomas, NGAL is strongly induced by NF-kB , an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development
Li et al., J Ethnopharmacol 2009 : The inhibitory effect of TMP on NF-kappaB ( p65 ) activity was mediated by blocking the consequent translocation of p65 into the nucleus
Guo et al., Mol Immunol 2009 (Hodgkin Disease) : TRAF1 is involved in the classical NF-kappaB activation and CD30 induced alternative activity in Hodgkin 's lymphoma cells ... The TRAF1-deficiency reduces the classical NF-kappaB activity but not the alternative activity ... Importantly, the CD30 induced alternative NF-kappaB activation is inhibited by the depletion of the TRAF1 expression ... Importantly, the CD30 induced alternative NF-kappaB activation is inhibited by the depletion of the TRAF1 expression
Pogue et al., J Inorg Biochem 2009 (Alzheimer Disease) : Characterization of an NF-kappaB regulated , miRNA-146a mediated down-regulation of complement factor H (CFH) in metal-sulfate stressed human brain cells
Koetzler et al., J Allergy Clin Immunol 2009 (Picornaviridae Infections) : To define the molecular mechanisms by which NO inhibits HRV-16 induced epithelial production of CXCL10 by affecting nuclear translocation and binding of nuclear factor-kappaB (NF-kappaB) and IFN regulatory factor 1 (IRF-1) ... In addition, the ability of HRV-16 to induce epithelial expression of IRF-1 is dependent , at least in part, on viral activation of NF-kappaB
Maloney et al., Arterioscler Thromb Vasc Biol 2009 : Activation of NF-kappaB by palmitate in endothelial cells : a key role for NADPH oxidase derived superoxide in response to TLR4 activation
Ho et al., J Immunol 2009 : NF-kappaB repressing factor (NRF) , a nuclear inhibitor of NF-kappaB , is constitutively expressed and is implicated in the basal silencing of specific NF-kappaB targeting genes, including IFN-beta, IL-8/CXCL8, and iNOS ... In contrast, NE did not induce NRF expression in A549 and Beas-2B cells, where NE only stimulates NF-kappaB activation and IL-8/CXCL8 induction ... In contrast, NE did not induce NRF expression in A549 and Beas-2B cells, where NE only stimulates NF-kappaB activation and IL-8/CXCL8 induction
Janjetovic et al., PloS one 2009 (Inflammation) : In keratinocytes lacking vitamin D receptor (VDR), 20-hydroxycholecalciferol did not affect IkappaB alpha mRNA levels, indicating that it requires VDR for its action on NF-kappaB activity
Sabolek et al., Stem Cells 2009 (MAP Kinase Signaling System) : Surprisingly, IL-1beta did not activate the NF-kappaB pathway or the transcription factor activating protein 1 (AP-1), but inhibition of nuclear translocation of NF-kappaB by SN50 facilitated IL-1beta induced Nurr1 expression and dopaminergic differentiation of mdNPCs ... Surprisingly, IL-1beta did not activate the NF-kappaB pathway or the transcription factor activating protein 1 (AP-1) , but inhibition of nuclear translocation of NF-kappaB by SN50 facilitated IL-1beta induced Nurr1 expression and dopaminergic differentiation of mdNPCs ... Surprisingly, IL-1beta did not activate the NF-kappaB pathway or the transcription factor activating protein 1 (AP-1), but inhibition of nuclear translocation of NF-kappaB by SN50 facilitated IL-1beta induced Nurr1 expression and dopaminergic differentiation of mdNPCs ... Surprisingly, IL-1beta did not activate the NF-kappaB pathway or the transcription factor activating protein 1 (AP-1), but inhibition of nuclear translocation of NF-kappaB by SN50 facilitated IL-1beta induced Nurr1 expression and dopaminergic differentiation of mdNPCs
Zhang et al., Experimental biology and medicine (Maywood, N.J.) 2009 : TRPC6 up-regulation in Ang II-induced podocyte apoptosis might result from ERK activation and NF-kappaB translocation ... The activation of ERK pathway and subsequent translocation of NF-kappaB was possibly necessary for the up-regulation TRPC6 induced by Ang II
Wang et al., Int J Cancer 2010 (Cell Transformation, Neoplastic...) : This was associated with significantly lower proliferating cell nuclear antigen positive hepatocytes and cyclinD1 protein, as well as decreased activation of extracellular signal regulated kinase and nuclear NF-kappaB ... This was associated with significantly lower proliferating cell nuclear antigen positive hepatocytes and cyclinD1 protein, as well as decreased activation of extracellular signal regulated kinase and nuclear NF-kappaB
Yang et al., J Immunol 2009 : GC-induced GILZ expression and GC inhibition of NF-kappaB activation were restored by expression of ANXA1 in ANXA1 ( -/- ) cells, and GILZ overexpression in ANXA1 ( -/- ) macrophages reduced ERK MAPK phosphorylation and restored sensitivity of cytokine expression and NF-kappaB activation to GC ... GC-induced GILZ expression and GC inhibition of NF-kappaB activation were restored by expression of ANXA1 in ANXA1 ( -/- ) cells, and GILZ overexpression in ANXA1 ( -/- ) macrophages reduced ERK MAPK phosphorylation and restored sensitivity of cytokine expression and NF-kappaB activation to GC
Du et al., Am J Physiol Cell Physiol 2009 : Similarly, in isolated peritoneal macrophages, roscovitine strongly inhibited ( * ) NO production, iNOS, and COX-2 upregulation, activation of NFkappaB , and induction of GCH-1 by LPS ... Similarly, in isolated peritoneal macrophages, roscovitine strongly inhibited ( * ) NO production, iNOS, and COX-2 upregulation, activation of NFkappaB , and induction of GCH-1 by LPS
Gao et al., Immunopharmacol Immunotoxicol 2009 : The suppression of these cell mediated immune responses by XN was at, least in part, due to the inhibition of nuclear factor kappa B (NF-kappaB) transcription factor through suppression of phosphorylation of IkappaBalpha , an inhibitor of NF-kappaB
Shan et al., Mol Cell Biochem 2009 : Here, we report that overexpression of RIOK3 inhibits TNFalpha induced NF-kappaB activation, but down-regulation of endogenous RIOK3 expression by siRNA potentiates it ... Here, we report that overexpression of RIOK3 inhibits TNFalpha induced NF-kappaB activation, but down-regulation of endogenous RIOK3 expression by siRNA potentiates it ... Interestingly, our data demonstrated that RIOK3 suppressed caspase-10 mediated NF-kappaB activation by competing RIP1 and NIK to bind to caspase-10 ... Interestingly, our data demonstrated that RIOK3 suppressed caspase-10 mediated NF-kappaB activation by competing RIP1 and NIK to bind to caspase-10
Wagner et al., Exp Dermatol 2010 : Furthermore, SFN elevated cellular glutathione levels and antagonized tumor necrosis factor-alpha induced NFkappaB transactivation
Lüthi et al., Immunity 2009 : Furthermore, caspase dependent processing of IL-33 was not required for ST2 receptor binding or ST2 dependent activation of the NF-kappaB transcription factor
Lee et al., Biochem Biophys Res Commun 2009 : In addition, TNF-alpha increased nuclear factor-kappa B (NF-kappaB) activity in NK cells and inhibition of NF-kappaB impeded TNF-alpha enhanced NK cell maturation
Zhou et al., Gastroenterology 2009 (Colitis...) : FoxO4 inhibits NF-kappaB and protects mice against colonic injury and inflammation ... Mechanistically, FoxO4 inhibited the transcriptional activity of nuclear factor-kappaB (NF-kappaB) , and Foxo4 deficiency is associated with increased NF-kappaB activity in vivo ... Mechanistically, FoxO4 inhibited the transcriptional activity of nuclear factor-kappaB (NF-kappaB) , and Foxo4 deficiency is associated with increased NF-kappaB activity in vivo
Doz et al., J Biol Chem 2009 (Tuberculosis) : PIM inhibited LPS/TLR4 induced NFkappaB translocation ... PIM inhibited LPS/TLR4 induced NFkappaB translocation ... PIM inhibited LPS/TLR4 induced NFkappaB translocation
Yamazaki et al., J Immunol 2009 : Following the degradation of GRP78 , transient activation of NF-kappaB was observed with a peak at 6-12 h ; the activation subsided within 24 h despite the continuous absence of intact GRP78 ... Dominant negative inhibition of IRE1alpha, XBP1 , or PERK did not attenuate activation of NF-kappaB by SubAB ... Dominant negative inhibition of IRE1alpha , XBP1, or PERK did not attenuate activation of NF-kappaB by SubAB ... In contrast, genetic and pharmacological inhibition of ATF6 significantly suppressed SubAB triggered Akt phosphorylation and NF-kappaB activation ... These results suggested that loss of GRP78 by SubAB leads to transient phosphorylation of Akt and consequent activation of NF-kappaB through the ATF6 branch of the UPR ... These results suggested that loss of GRP78 by SubAB leads to transient phosphorylation of Akt and consequent activation of NF-kappaB through the ATF6 branch of the UPR
Mandrekar et al., J Immunol 2009 (Acute Disease...) : In summary, inhibition of LPS induced NFkappaB and ERK activation by acute alcohol leads to hyporesponsiveness of monocytes to LPS due to increased IRAK-M
Guo et al., Int J Exp Pathol 2009 (Reperfusion Injury) : From day 3 to day 6 after reperfusion, the accumulation of CSCs was significantly elevated in the injured area, which was matched with the increased SCF expression during I/R. Pretreatment of rats with NF-kappaB inhibitor, N-acetyl-L-cysteine (NAC) not only suppressed NF-kappaB activation induced by I/R but also attenuated SCF expression
Kim et al., J Cell Biochem 2009 : On the contrary, eupatilin dramatically inhibited I-kappaBalpha degradation and NF-AT dephosphorylation and, consequently, inhibited NF-kappaB and NF-AT promoter activities in PMA/A23187 stimulated T cells
Correa et al., Glia 2010 (MAP Kinase Signaling System) : AEA also inhibits NF-kappaB activation by interfering with the phosphorylation of IkappaBalpha , which may result in an increase of IL-10 production
Malaver et al., Journal of thrombosis and haemostasis : JTH 2009 : Two specific unrelated inhibitors of NF-kappaB activation, BAY 11-7082 and Ro 106-9920, reduced PAC-1 and fibrinogen binding to integrin alpha ( IIb ) beta3 and restricted platelet spreading on immobilized fibrinogen ... Two specific unrelated inhibitors of NF-kappaB activation, BAY 11-7082 and Ro 106-9920, reduced PAC-1 and fibrinogen binding to integrin alpha ( IIb ) beta3 and restricted platelet spreading on immobilized fibrinogen
Choi et al., J Endod 2009 (Pulpitis...) : SLPI also inhibited the LPS induced activation of NF-kappaB in MDPC-23 ... SLPI also inhibited the LPS induced activation of NF-kappaB in MDPC-23
Ayroldi et al., FASEB J 2009 : The identification of GILZ interaction with and inhibition of NF-kappaB provided a first molecular mechanistic basis for explaining GILZ effects on T cells
Lee et al., J Biol Chem 2009 (Inflammatory Bowel Diseases) : In support for this, we identified a role for SLC15A4 , an oligopeptide transporter expressed in early endosomes, in Nod1 dependent NF-kappaB signaling ... In support for this, we identified a role for SLC15A4, an oligopeptide transporter expressed in early endosomes, in Nod1 dependent NF-kappaB signaling
Thomson et al., Proc Natl Acad Sci U S A 2009 : We also show that depletion of the NF-kappaB RelA protein significantly reduces the level of the IFN-lambda1 gene expression ... We conclude that IFN-lambda1 gene expression requires NF-kappaB, and we propose a model for IFN-lambda1 gene regulation, in which IRF and NF-kappaB activate gene expression independently via spatially separated promoter elements ... These observations provide insights into the independent evolution of the IFN-lambda1 and IFN-beta promoters and directly implicate transposable elements in the regulation of the IFN-lambda1 gene by NF-kappaB
Ishida et al., Inflamm Bowel Dis 2010 (Colitis...) : In HEK293 cells, pretreatment with RvE1 inhibited TNF-alpha induced nuclear translocation of NF-kappaB in a ChemR23 dependent manner
Chiu et al., Chem Biol Interact 2009 : Propofol inhibits lipoteichoic acid induced iNOS gene expression in macrophages possibly through downregulation of toll-like receptor 2-mediated activation of Raf-MEK1/2-ERK1/2-IKK-NFkappaB
Brownlie et al., Mol Immunol 2009 : In this study, chicken TLRs 7, 15 and 21 were expressed in mammalian HEK-293T cells ; expression of TLR21 but not TLR7 or 15 resulted in marked NF-kappaB activation upon stimulation with exogenous ODN ... In this study, chicken TLRs 7, 15 and 21 were expressed in mammalian HEK-293T cells ; expression of TLR21 but not TLR7 or 15 resulted in marked NF-kappaB activation upon stimulation with exogenous ODN ... TLR21 mediated NF-kappaB activation in HEK-293T cells was inhibited by bafilomycin A suggesting that endosomal maturation is required for TLR21 activation and observations by confocal microscopy and digestion with endoglycosidase H suggest TLR21 localizes to the endoplasmic reticulum ( ER ) of resting cells
Ju et al., Biochem Biophys Res Commun 2009 : PGG significantly inhibited TNF-alpha/IFN-gamma induced NF-kappaB activation as well as STAT1 activation ... PGG significantly inhibited TNF-alpha/IFN-gamma induced NF-kappaB activation as well as STAT1 activation ... These results suggest that PGG may exert anti-inflammatory responses by suppressing TNF-alpha and/or IFN-gamma induced activation of NF-kappaB and STAT1 in the keratinocytes and might be a useful tool in therapy of skin inflammatory diseases
Lauksund et al., Dev Comp Immunol 2009 (Birnaviridae Infections) : Atlantic salmon IPS-1 mediates induction of IFNa1 and activation of NF-kappaB and localizes to mitochondria ... Atlantic salmon IPS-1 mediates induction of IFNa1 and activation of NF-kappaB and localizes to mitochondria
Cadera et al., J Exp Med 2009 : Because IRF4 is a target of NF-kappaB and is required for receptor editing, we suggest that NF-kappaB could be acting through IRF4 to regulate receptor editing
Fujita et al., Results Probl Cell Differ 2009 (Inflammation...) : Ubiquitin protein ligases ( E3s ), DUBs, and signaling molecules with ubiquitin recognition motifs control TNFR1 mediated cell death and activation of NF-kappaB and JNK ... Ubiquitin protein ligases ( E3s ), DUBs, and signaling molecules with ubiquitin recognition motifs control TNFR1 mediated cell death and activation of NF-kappaB and JNK
Resch et al., J Cell Sci 2009 : Reporter-gene analysis revealed that Siva1 inhibits XIAP- and TAK1-TAB1 mediated NFkappaB activation ... Our findings suggest that, via physical interaction with XIAP and TAK1, Siva1 diminishes NFkappaB and enhances JNK activity to favour apoptosis
Choi et al., J Cell Sci 2009 : This activity was confirmed by the AIMP2 dependent increase of IkappaB and suppression of NFkappaB
Hinojosa et al., J Infect Dis 2009 (Inflammation...) : During pneumonia, aged mice had reduced levels of pIgR and PAFr and less NFkB activation , despite greater bacterial burden ... During pneumonia, aged mice had reduced levels of pIgR and PAFr and less NFkB activation , despite greater bacterial burden
Dagvadorj et al., Innate Immun 2009 : Further, IL-10 inhibited LPS induced NF-kappaB activation ... Further, IL-10 inhibited LPS induced NF-kappaB activation ... A pharmacological inhibitor of NF-kappaB prevented LPS induced IkappaB-zeta expression, suggesting that IL-10 might inhibit LPS induced IkappaB-zeta expression via the inactivation of NF-kappaB
Petzold et al., Am J Physiol Cell Physiol 2009 : NF-kappaB activation by TNF-alpha or hydrogen peroxide was FAK independent
Wysoczynski et al., Int J Cancer 2010 (Anoxia...) : This suggests that IL-8 expression is regulated in an activating protein (AP)-1- and nuclear factor (NF)-kappaB dependent manner
Nakano et al., Hypertens Res 2009 : Telmisartan caused a dose dependent suppression of the tumor necrosis factor-alpha (TNFalpha) induced activation of nuclear factor (NF)-kappaB in vascular endothelial cells in this study ... The thiazolidinediones neither influenced TNFalpha induced NF-kappaB activation nor influenced the inhibitory effect of telmisartan in this process ... Thus, telmisartan inhibits the cytokine induced expression of the VCAM-1 gene by blocking NF-kappaB activation independently of PPAR gamma activation
Chuong et al., J Cell Physiol 2009 : The aim of this study was to investigate the inhibitory effect of the soluble-RAGE ( sRAGE ), the extracellular domain of RAGE, on RAGE expression and NF-kappaB translocation in human-salivary gland-cell-lines ( HSG )
Tao et al., Curr Biol 2009 (Crohn Disease) : We also show that NOD2 can bind polyubiquitinated RIP2 and that whereas ITCH E3 ligase activity is required for optimal NOD2 : RIP2 induced p38 and JNK activation, ITCH inhibits NOD2 : RIP2 induced nuclear factor kappa B (NFkappaB) activation
Wort et al., J Biol Chem 2009 : Synergistic induction of endothelin-1 by tumor necrosis factor alpha and interferon gamma is due to enhanced NF-kappaB binding and histone acetylation at specific kappaB sites
Verstak et al., J Biol Chem 2009 (Bacterial Infections...) : MyD88 adapter-like (Mal)/TIRAP interaction with TRAF6 is critical for TLR2- and TLR4 mediated NF-kappaB proinflammatory responses ... MyD88 adapter-like (Mal)/TIRAP interaction with TRAF6 is critical for TLR2- and TLR4 mediated NF-kappaB proinflammatory responses ... MyD88 adapter-like (Mal)/TIRAP interaction with TRAF6 is critical for TLR2- and TLR4 mediated NF-kappaB proinflammatory responses ... We have previously reported an interaction between Mal and tumor necrosis factor receptor associated factor 6 ( TRAF6 ) via a TRAF6 binding motif, the disruption of which inhibited TLR mediated NF-kappaB-luciferase reporter activity ... Furthermore, Mal interaction with TRAF6 mediates Ser phosphorylation of the p65 subunit of NF-kappaB and thus controls transcriptional activation but not nuclear translocation of NF-kappaB ... Furthermore, Mal interaction with TRAF6 mediates Ser phosphorylation of the p65 subunit of NF-kappaB and thus controls transcriptional activation but not nuclear translocation of NF-kappaB
Funakoshi-Tago et al., Mol Pharmacol 2009 : Here, we found that licochalcone A strongly inhibited tumor necrosis ( TNF)-alpha induced nuclear localization, DNA binding activity, and the transcriptional activity of nuclear factor-kappaB (NF-kappaB) ... In contrast, a structurally related compound, echinatin, failed to inhibit TNF-alpha induced IKK activation and NF-kappaB activation, suggesting that the 1,1-dimethy-2-propenyl group in licochalcone A is important for the inhibition of NF-kappaB
Hu et al., J Immunol 2009 (Cryptosporidiosis...) : Moreover, gain-of-function ( by overexpression of CIS ) and loss-of-function ( by siRNA interference ) studies revealed that CIS could enhance IkappaBalpha degradation and regulate NF-kappaB activation in cholangiocytes in response to LPS stimulation or C. parvum infection
Qi et al., Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 2009 : Curcumin can restrain expression of MMP-2 and MMP-9 in cystiform tissue of air-pouch animal models, and expression of MMP-2 and MMP-9 may be regulated by the activation of NF-kappaB ... Curcumin can restrain expression of MMP-2 and MMP-9 in cystiform tissue of air-pouch animal models, and expression of MMP-2 and MMP-9 may be regulated by the activation of NF-kappaB
Henmi et al., Biochem J 2009 : Although the molecular mechanism for the IL-1 induced activation of NF-kappaB has been well documented, much less is known about the mechanism by which protein phosphatases down-regulate this pathway ... Ectopic expression of PP2Ceta-2 in human embryonic kidney HEK293IL-1RI cells inhibited the IL-1 induced activation of NF-kappaB ... Ectopic expression of PP2Ceta-2 in human embryonic kidney HEK293IL-1RI cells inhibited the IL-1 induced activation of NF-kappaB ... TAK1 ( transforming-growth-factor-beta activated kinase 1 ) mediates the IL-1 signalling pathway to NF-kappaB, and we observed that the TAK1 induced activation of NF-kappaB was suppressed by PP2Ceta-2 expression ... TAK1 ( transforming-growth-factor-beta activated kinase 1 ) mediates the IL-1 signalling pathway to NF-kappaB, and we observed that the TAK1 induced activation of NF-kappaB was suppressed by PP2Ceta-2 expression ... Expression of IKKbeta [ IkappaB ( inhibitory kappaB ) kinase beta ], which lies downstream of TAK1, activates NF-kappaB , and this activation was also readily reversed by PP2Ceta-2 co-expression
Qi et al., Br J Pharmacol 2009 : Fluvastatin, but not atorvastatin or simvastatin, inhibited MDC expression induced by interferon (IFN)-gamma and NF-kappaB activation ... A NF-kappaB inhibitor, but not a STAT1 inhibitor, suppressed MDC expression in HaCaT cells ... Further, inhibition of p38 mitogen activated protein kinases ( MAPKs ) significantly suppressed IFN-gamma induced MDC expression and NF-kappaB activation ... Further, inhibition of p38 mitogen activated protein kinases ( MAPKs ) significantly suppressed IFN-gamma induced MDC expression and NF-kappaB activation ... Interestingly, fluvastatin suppressed IFN-gamma induced NF-kappaB activation in parallel with p38 MAPK phosphorylation ... These results indicate that fluvastatin inhibited expression of the CC chemokine MDC induced by IFN-gamma in HaCaT cells, by inhibiting NF-kappaB activation via the p38 MAPK pathway
Treede et al., BMC gastroenterology 2009 : It could be demonstrated that the exogenous application of PC inhibits membrane dependent actin assembly and TNF-alpha induced nuclear NF-kappaB activation ... We analysed TNF-alpha induced NF-kappaB-activation via the transient expression of a NF-kappaB-luciferase reporter system
Jung et al., Free Radic Biol Med 2009 (Inflammation) : Thus, our data suggest, for the first time, that the delicate balance between PTK and PTP is disturbed during aging and inflammation, both of which lead to NF-kappaB activation via NIK/IKK and MAPKs
Baudy et al., Int Immunopharmacol 2009 (Myositis) : Since NF-kappaB is an important therapeutic target, we decided to utilize an in vitro screening assay to identify potential inhibitors that block TNF-alpha induced NF-kappaB activation in a C2C12 muscle line stably expressing an NF-kappaB luciferase reporter gene
Cheng et al., Infect Immun 2009 (Inflammation) : While activation of NF-kappaB was essential for heat inactivated S. aureus induced TNF-alpha and NO, inhibiting GSK-3beta blocked heat inactivated S. aureus induced NF-kappaB p65 nuclear translocation ... While activation of NF-kappaB was essential for heat inactivated S. aureus induced TNF-alpha and NO, inhibiting GSK-3beta blocked heat inactivated S. aureus induced NF-kappaB p65 nuclear translocation ... These results suggest that GSK-3beta regulates heat inactivated S. aureus induced TNF-alpha and NO production in microglia mainly by activating NF-kappaB and probably by inhibiting IL-10 ... These results suggest that GSK-3beta regulates heat inactivated S. aureus induced TNF-alpha and NO production in microglia mainly by activating NF-kappaB and probably by inhibiting IL-10 ... These results suggest that GSK-3beta regulates heat inactivated S. aureus induced TNF-alpha and NO production in microglia mainly by activating NF-kappaB and probably by inhibiting IL-10
Koarai et al., Am J Respir Cell Mol Biol 2010 : One hundred micromoles of H ( 2 ) O ( 2 ) enhanced the translocation of nuclear factor (NF)-kappaB p65, but not that of interferon regulatory factor-3 (IRF-3), into the nucleus and the NF-kappaB DNA binding activity after poly ( I:C ) stimulation, which effect was inhibited not by the silencing of IRF-3 but by MG132, a proteasome inhibitor, or dexamethasone ... One hundred micromoles of H ( 2 ) O ( 2 ) enhanced the translocation of nuclear factor (NF)-kappaB p65 , but not that of interferon regulatory factor-3 (IRF-3), into the nucleus and the NF-kappaB DNA binding activity after poly ( I:C ) stimulation, which effect was inhibited not by the silencing of IRF-3 but by MG132, a proteasome inhibitor, or dexamethasone
Cai et al., J Cell Mol Med 2009 (Inflammation) : At the molecular level, GTS inhibited p65RelA but not RelB NF-kappaB during resuscitation
Nakamura et al., J Periodontal Res 2010 (Alveolar Bone Loss) : Furthermore, when GTC was added to the medium, decreased responses to LPS were observed in CD14 expressing Chinese hamster ovary ( CHO ) reporter cells, which express CD25 through LPS induced nuclear factor-kappaB (NF-kappaB) activation
Reid et al., Molecular cancer 2009 (Breast Neoplasms...) : These increases in endothelial cell OPG secretion were dependent on alphanubeta3 ligation and NFkappaB activation
Chun et al., J Leukoc Biol 2009 : This Ca2+ release plays a pivotal role in signaling TLR2 dependent NF-kappaB activation and chemokine expression to recruit PMNs to the lung
Zhang et al., Journal of translational medicine 2009 (Diabetes Mellitus, Experimental...) : Inhibition of nuclear factor kappa B (NF-kappaB) activation induced by IL-1beta and IFN-gamma was investigated ... Inhibition of nuclear factor kappa B (NF-kappaB) activation induced by IL-1beta and IFN-gamma was investigated
Campo et al., Biochim Biophys Acta 2009 : HA treatment produced different effects : low MW HA up-regulated CD44 expression, increased PKCdelta and PKCepsilon levels, and enhanced inflammation in untreated chondrocytes ; while in PMA treated cells it increased CD44, PKCdelta, PKCepsilon, NF-kB , TNF-alpha, IL-1beta, MMP-13, and iNOS expression and enhanced the effects of PMA ; medium MW HA did not exert action ; high MW HA had no effect on untreated chondrocytes ; however, it reduced PKCdelta, PKCepsilon, NF-kB activation and inflammation in PMA stimulated cells
Correa-de-Santana et al., J Endocrinol 2009 : Furthermore, silencing STAT3 inhibited basal, LPS and MDP stimulated NFKB protein expression and overexpression of protein inhibitor of activated STAT3 ( Pias3 ) markedly decreased basal NFKB activity ... Furthermore, silencing STAT3 inhibited basal, LPS and MDP stimulated NFKB protein expression and overexpression of protein inhibitor of activated STAT3 ( Pias3 ) markedly decreased basal NFKB activity ... Furthermore, silencing STAT3 inhibited basal, LPS and MDP stimulated NFKB protein expression and overexpression of protein inhibitor of activated STAT3 ( Pias3 ) markedly decreased basal NFKB activity ... Furthermore, silencing STAT3 inhibited basal, LPS and MDP stimulated NFKB protein expression and overexpression of protein inhibitor of activated STAT3 ( Pias3 ) markedly decreased basal NFKB activity
Journo et al., PLoS Pathog 2009 : Tax1 activation of NF-kappaB occurs predominantly in the cytoplasm, where Tax1 binds NF-kappaB Essential Modulator ( NEMO/IKKgamma ) and triggers the activation of IkappaB kinases ... Several independent studies have shown that Tax1 mediated NF-kappaB activation is dependent on Tax1 ubiquitination ... In addition, we observe that NRP increases the ubiquitination of Tax1 along with Tax1 dependent NF-kappaB signaling
Bai et al., Int J Cancer 2009 (Cell Transformation, Neoplastic) : Akt mediated regulation of NFkappaB and the essentialness of NFkappaB for the oncogenicity of PI3K and Akt ... Previous studies suggest that Akt regulates transcriptional activity of the nuclear factor-kappaB (NFkappaB) by inducing phosphorylation and subsequent degradation of inhibitor of kappaB ( IkappaB ) ... Our data suggest that the stimulation of NFkappaB by Akt is dependent on the phosphorylation of p65 at S534, mediated by IKK ( IkappaB kinase) alpha and beta ... Our data suggest that the stimulation of NFkappaB by Akt is dependent on the phosphorylation of p65 at S534, mediated by IKK ( IkappaB kinase) alpha and beta ... The data further support the conclusion that NFkappaB activity is essential for PI3K- and Akt induced oncogenic transformation
Abdulamir et al., J Investig Allergol Clin Immunol 2009 (Asthma) : NF-kappaB seems to be the central inflammatory factor in SA, with a remarkable loss of PBL apoptosis, diminished Treg levels, and high CD30+ cell levels that probably induce NF-kappaB , which in turn blocks the proapoptotic potential of CD30 induction itself
Liao et al., Toxicol Lett 2009 : In studying the mechanisms involved in alpha-Triol induced COX-2/PGE ( 2 ) production, inhibitors of NOS, PI3K, p38, and NF-kappaB, effectively attenuated COX-2 protein induction and mRNA expression, suggesting that the PI(3)K-Akt-eNOS pathway, p38MAPK, and NF-kappaB are involved in alpha-Triol induced COX-2 expression, and following increases in p38 and Akt phosphorylation, the concentration dependent inhibition of COX-2 protein expression by L-NAME further suggested their involvement at the translation level
Lin et al., Cell Signal 2009 : These results suggested that in A549 cells, the activation of p42/p44 MAPK, p38 MAPK, JNK1/2, NF-kappaB , and AP-1 are essential for the IL-1 beta induced MMP-9 gene expression and cell migration
Yuan et al., Eur J Pharmacol 2009 (Inflammation...) : Ghrelin also significantly suppressed interleukin-1beta, tumor necrosis factor-alpha , and endothelin-l mRNA expression, and inhibited NF-kappaB activation ... Ghrelin also significantly suppressed interleukin-1beta, tumor necrosis factor-alpha, and endothelin-l mRNA expression, and inhibited NF-kappaB activation ... Ghrelin also significantly suppressed interleukin-1beta , tumor necrosis factor-alpha, and endothelin-l mRNA expression, and inhibited NF-kappaB activation
Shih et al., Eur J Pharmacol 2009 : In this study, we investigated the involvement of the extracellular signal regulated kinase ( ERK ), IkappaB kinase alpha/beta ( IKKalpha/beta ), and nuclear factor-kappaB (NF-kappaB) signaling pathways in thrombin induced COX-2 expression in human lung fibroblasts ( WI-38 )
Joo et al., Eur J Pharmacol 2009 (Colitis) : An anti-inflammatory mechanism of taurine conjugated 5-aminosalicylic acid against experimental colitis : taurine chloramine potentiates inhibitory effect of 5-aminosalicylic acid on IL-1beta mediated NFkappaB activation ... To explore a molecular mechanism for the anti-inflammatory property of the prodrug, we investigated the effect of the conjugate on IL-1beta mediated NFkappaB activation ... Furthermore, combined TauCl/5-ASA treatment interfered additively with the activation process, leading to additive inhibitory effect on IL-1beta mediated NFkappaB activation ... Our results suggest that the anti-inflammatory effect of the prodrug on experimental colitis is attributed to the inhibition of the IL-1beta mediated NFkappaB activation and the taurine effect is through TauCl potentiating the ability of 5-ASA to inhibit IL-1beta dependent NFkappaB activation ... Our results suggest that the anti-inflammatory effect of the prodrug on experimental colitis is attributed to the inhibition of the IL-1beta mediated NFkappaB activation and the taurine effect is through TauCl potentiating the ability of 5-ASA to inhibit IL-1beta dependent NFkappaB activation
Ghosh et al., Toxicol Appl Pharmacol 2009 (MAP Kinase Signaling System) : Pre-treatment with SP600125 ( JNK inhibitor ) and SB203580 ( p38 MAPK inhibitor ) attenuated NF-kappaB and IKK phosphorylation indicating that p38 and JNK MAPKs are mainly involved in arsenic induced NF-kappaB activation
Farmer et al., Pharmacol Ther 2009 (Disease Models, Animal...) : RAGE is upregulated as a consequence of activation of the ubiquitous pro-inflammatory transcription factor NF-kappaB which is activated in response to diverse inflammatory stimuli including hyperglycaemia, oxidised low density lipoprotein ( oxLDL ) and reduced shear stress ... RAGE binding by circulating advanced glycation endproducts ( AGEs ) or S100 protein released by activated leukocytes results in the generation of reactive oxygen species ( ROS ) and further activation of NF-kappaB
Zapolska-Downar et al., J Physiol Pharmacol 2009 : In addition, propionate significantly inhibited the TNF-alpha induced activation of nuclear factor-kappa B (NF-kappaB) and significantly increased the expression of peroxisome proliferator activated receptor alpha ( PPARalpha ) in HUVEC
Di et al., Mol Biol Rep 2010 (Prostatic Neoplasms) : The regulatory role of NF-kappaB in TLR9 mediated COX-2 expression was also investigated
Kim et al., Journal of translational medicine 2009 (Ascites...) : Unlike Paclitaxel, ARRY-520 did not induce NF-kappaB activation, did not enhance cytokine secretion, nor induce ERK phosphorylation in Type I EOC cells
Zhang et al., Dev Cell 2009 : We find that Wnt/beta-catenin signaling is absolutely required for NF-kappaB activation, and that Edar is a direct Wnt target gene ... We find that Wnt/beta-catenin signaling is absolutely required for NF-kappaB activation, and that Edar is a direct Wnt target gene
Song et al., Blood 2009 (Endotoxemia) : Here, we used transgenic mice that conditionally overexpress a mutant I-kappaBalpha , an inhibitor of NF-kappaB , selectively on endothelium, and their wild-type littermates to define the role of endothelial-specific NF-kappaB in septic coagulation ... Endothelial NF-kappaB blockade prevented LPS down-regulation of endothelial protein C receptor ( EPCR ) and thrombomodulin protein expressions, inhibited tissue tumor necrosis factor-alpha converting enzyme activity, reduced EPCR shedding, and restored plasma protein C level ... Endothelial NF-kappaB blockade prevented LPS down-regulation of endothelial protein C receptor ( EPCR ) and thrombomodulin protein expressions, inhibited tissue tumor necrosis factor-alpha converting enzyme activity, reduced EPCR shedding, and restored plasma protein C level
Warfel et al., J Biol Chem 2009 (Anthrax...) : These data suggest that LT-mediated enhancement of NF-kappaB is IKK dependent , but importantly, the net effect of LT on the transcription of proinflammatory genes is driven by the cumulative effect of LT on the particular set of transcription factors that regulate a given promoter
Ji et al., Anticancer Drugs 2009 (Lung Neoplasms) : TNF-alpha treatment induced an elevated activated NFkappaB/p65 , concomitant with induced p21 levels, which resulted in increased sensitivity to gefitinib in PC-9-ZD cells ... Consistent with our earlier observation that p21 is induced in an NFkappaB/p65 dependent manner, we conclude that p21 plays an important role in mediating cell growth inhibition by gefitinib
Ikegaya et al., J Chemother 2009 : Ikappab-alpha , an inhibitor of NFkappab , was not disrupted by the antibiotics
Peric et al., PloS one 2009 (MAP Kinase Signaling System...) : All vitamin D analogs tested blocked IL-17A induced HBD2 expression by increasing IkappaB-alpha protein and inhibition of NF-kappaB signaling
Li et al., Am J Physiol Cell Physiol 2009 : Both IL-1 isoforms stimulate phosphorylation of p38 mitogen activated protein kinase and stimulate nuclear factor-kappaB (NF-kappaB) signaling ; I-kappaB levels fall and NF-kappaB DNA binding activity increases ... We conclude that IL-1alpha and IL-1beta act via an oxidant- and AKT/Foxo independent mechanism to activate p38 MAPK, stimulate NF-kappaB signaling, increase expression of atrogin1/MAFbx and MuRF1, and reduce myofibrillar protein in differentiated myotubes ... We conclude that IL-1alpha and IL-1beta act via an oxidant- and AKT/Foxo independent mechanism to activate p38 MAPK, stimulate NF-kappaB signaling, increase expression of atrogin1/MAFbx and MuRF1, and reduce myofibrillar protein in differentiated myotubes
Gordon et al., J Cell Physiol 2009 : Our data suggest TGF-beta mediated MMP-9 induction may be regulated by the NF-kappaB , Smad3, and JNK pathways, whereas the IL-1beta mediated induction may be regulated by the NF-kappaB and p38 pathways
Sun et al., Journal of molecular signaling 2009 : In this study, we have used stable melanoma cell lines, parental A2058, A2058T2-1 overexpressing TIMP-2, and A2058T2R-7 underexpressing TIMP-2, to determine the TIMP-2 regulation of the NF-kappaB activity ... TIMP-2 overexpressed cells had the lower basal level of IkappaBalpha , the inhibitor of NF-kappaB , compared to the parental A2058 cells ... The transcriptional NF-kappaB activity was increased by the TIMP-2 overexpression
Zhang et al., Am J Pathol 2009 (Prostatic Neoplasms) : NF-kappaB regulates androgen receptor expression and prostate cancer growth
Benson et al., PloS one 2009 : In addition, NF-kappaB signaling was required for inhibition of CXCL11 transcription, but not for inhibition of CIITA ... In addition, NF-kappaB signaling was required for inhibition of CXCL11 transcription, but not for inhibition of CIITA
Madonna et al., Exp Cell Res 2009 : Thus, Epo protects cardiac myocyte progenitors or myoblasts against the cytotoxic effects of TNF-alpha by inhibiting NF-kappaB mediated iNOS expression and NO production and by preventing caspase-3 activation
Sheng et al., Free Radic Biol Med 2009 (Disease Models, Animal...) : Mn ( III ) TDE-2-ImP ( 5+ ) also attenuated postischemic NF-kappaB activation ... Mn ( III ) TDE-2-ImP ( 5+ ) also attenuated postischemic NF-kappaB activation
Chen et al., FEBS Lett 2009 (MAP Kinase Signaling System) : CRP could also increase protein expression of phosphorylated nuclear factor-kappaB (NF-kappaB) , IkappaB-alpha and ERK1/2 in dose- and time dependent manner
Beraza et al., J Exp Med 2009 : The IKK complex mediates NF-kappaB activation and deletion of its regulatory subunit NEMO in hepatocytes ( NEMO ( Delta hepa ) ) triggers chronic inflammation and spontaneous hepatocellular carcinoma development
Saruta et al., Eur J Immunol 2009 : TLR8 recognizes ssRNA and induces NF-kappaB via MyD88 signaling
Ruben et al., New Biol 1990 (Burkitt Lymphoma) : Transient short-term expression of the Tax protein of human T-cell leukemia virus type-I ( HTLV-I ) leads to activation of the pleiotropic transcription factor NF-kappa B
Chen et al., American journal of physiology. Renal physiology 2009 (Reperfusion Injury) : PPAR-alpha reduced the NF-kappaB induced overexpression of TNF-alpha and apoptosis in cultured kidney cells ... This indicated that PPAR-alpha attenuated renal I/R injury via NF-kappaB induced TNF-alpha overexpression ... Taken together, our results demonstrate for the first time that prostacyclin induces the translocation of PPAR-alpha from the cytosol into the nucleus and attenuates NF-kappaB induced TNF-alpha activation following renal I/R injury
Park et al., J Immunol 2009 (Asthma...) : In addition, we found that administration of rosiglitazone or pioglitazone decreased the increased NF-kappaB activity and that a NF-kappaB inhibitor, BAY 11-7085, substantially reduced the increased IL-17 protein levels in the lung tissues after OVA inhalation
Farfsing et al., Leukemia 2009 (Leukemia, Lymphocytic, Chronic, B-Cell...) : Gene knockdown and a nuclear factor kappa B (NFkappaB) reporter gene assay revealed that CCDC50 is required for survival in MCL and CLL cells and controls NFkappaB signaling
Zhu et al., J Interferon Cytokine Res 2009 : DCs treated with HMGB1 ( 1 microg/mL for 48 h ) promoted T-cell proliferation as well as differentiation, and markedly up-regulated IL-2, IL-2R expression and intranuclear NF-kappaB activation
King et al., J Biol Chem 2009 : Thus, MKP-1 attenuates TNFalpha dependent activation of p38 MAPK, induction of IL-8 expression, and NF-kappaB dependent transcription
Zhang et al., J Biol Chem 2009 : Injury induced platelet derived growth factor receptor-alpha expression mediated by interleukin-1beta (IL-1beta) release and cooperative transactivation by NF-kappaB and ATF-4 : IL-1beta facilitates HDAC-1/2 dissociation from promoter ... ATF-4 and NF-kappaB interact, occupy the PDGF-R alpha promoter, and induce PDGF-R alpha transcription in a cooperative manner
Xiao et al., J Infect Dis 2009 (Helicobacter Infections...) : H. pylori was able to increase the miR-155 expression in gastric epithelial cell lines and gastric mucosal tissues, and nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) pathway were required for the induction of miR-155
Sasaki et al., J Biol Chem 2009 : Intriguingly, both Ci-TLR1 and Ci-TLR2 stimulate NF-kappaB induction in response to multiple pathogenic ligands such as double stranded RNA, and bacterial cell wall components that are differentially recognized by respective vertebrate TLRs, revealing that Ci-TLRs recognize broader pathogen associated molecular patterns than vertebrate TLRs ... Intriguingly, both Ci-TLR1 and Ci-TLR2 stimulate NF-kappaB induction in response to multiple pathogenic ligands such as double stranded RNA, and bacterial cell wall components that are differentially recognized by respective vertebrate TLRs, revealing that Ci-TLRs recognize broader pathogen associated molecular patterns than vertebrate TLRs
Lv et al., Thromb Haemost 2009 : Moreover, HMGB1 increased expression of Egr-1 and nuclear translocation of NF-kappaB ( c-Rel/p65 ) in ECs ... Moreover, HMGB1 increased expression of Egr-1 and nuclear translocation of NF-kappaB ( c-Rel/p65 ) in ECs ... Taken together, our data suggest that HMGB1 induces TF expression in vascular endothelial cells via cell surface receptors ( TLR4, TLR2, and RAGE ), and through activation of transcription factors ( NF-kappaB and Egr-1 )
Wang et al., Phytother Res 2010 : TNF-alpha could enhance the activity of nuclear factor-kappa B (NF-kappaB) in the foam cells
Kim et al., J Cell Physiol 2009 (Bone Resorption) : Interestingly, saurolactam prevented RANKL induced activation of MAP kinases and NF-kappaB , and mRNA expression of osteoclast related genes and transcription factors ( c-Fos, Fra-2, and NFATc1 )
Schichl et al., J Biol Chem 2009 : Tristetraprolin impairs NF-kappaB/p65 nuclear translocation ... Accordingly, TTP is involved in the negative feedback regulation of NF-kappaB through promoting mRNA degradation
Ma et al., Am J Chin Med 2009 (Arthritis, Gouty) : In addition, EPS ( 2.25, 4.5 g/kg, ig 9 days ) inhibited the expression of TNF-alpha and IL-1beta and the biological activity of NF-kappaB
Piao et al., J Leukoc Biol 2009 : To examine functional consequences of increased expression of Tollip in LPS tolerized cells, we overexpressed Tollip in 293/TLR4/MD-2 transfectants and observed blunted LPS-inducible activation of NF-kappaB and RANTES, while TNF-alpha responses were not affected
An et al., American journal of physiology. Renal physiology 2009 (Fibrosis...) : The untreated CRF rats exhibited proteinuria, hypertension, azotemia, upregulations of renal tissue NAD ( P ) H oxidase, MCP-1, COX-2, PAI-1, TGF-beta, Smad2, alpha-smooth muscle actin, fibronectin, and hepatocyte growth factor, activation of ERK1/2 and NF-kappaB , downregulation of Smad7 , intense mononuclear leukocyte infiltration, tubulointerstitial fibrosis, and glomerulosclerosis
Lin et al., Free Radic Biol Med 2009 (Inflammation) : Furthermore, prefeeding LGG prevented TNF-alpha induced intestinal NF-kappaB activation
Shi et al., Am J Physiol Gastrointest Liver Physiol 2009 (Colitis...) : The translocation of NF-kappaB was inhibited by blocking the degradation of IkappaBbeta
Hiroi et al., Arterioscler Thromb Vasc Biol 2009 : Thrombomodulin upregulation was independent of NF-kappaB signaling, a principal target of proteasome inhibitors, but was instead a direct consequence of increased expression of the Krüppel-like transcription factors, KLF2 and KLF4
Sheng et al., J Huazhong Univ Sci Technolog Med Sci 2009 : The relation between the expression and activity of MMP-9 in C-reactive protein (CRP) induced human THP-1 mononuclear cells and the activation of nuclear factor kappa-B (NF-kappaB) was studied to investigate the possible role of CRP in plaque destabilization
Liang et al., J Huazhong Univ Sci Technolog Med Sci 2009 : The results showed that in G1, G2 and G3 groups, insulin secretion was enhanced with the increase of glucose concentration, and the NF-kappaB expression was also increased ( P < 0.05 ), but Bax activity, Cyt C release and apoptosis rate showed no significant difference among them ... However, in G4, G5, and G6 groups, apoptosis rate of islet cells, NF-kappaB expression, Bax activity, and Cyt C release were all significantly increased, and insulin secretion was impaired as compared with G1, G2, and G3 groups ( P < 0.05 )
Monsalve et al., Eur J Immunol 2009 : We report here that Notch signaling increases both basal and LPS induced NF-kappaB activation, favoring the expression of genes implicated in the inflammatory response, such as the cytokines TNF-alpha and IL-6, or enzymes, such as iNOS ... We report here that Notch signaling increases both basal and LPS induced NF-kappaB activation, favoring the expression of genes implicated in the inflammatory response, such as the cytokines TNF-alpha and IL-6, or enzymes, such as iNOS
Ke et al., Neurosci Res 2009 (Gliosis...) : A2aR-selective antagonist Sch58261 decreased GFAP expression in a dose- and time dependent manner with increased activation of Akt , and induced activation of NF-kappaB
Yun et al., Int Immunopharmacol 2009 : Furthermore, molecular data revealed that pseudocoptisine inhibited the LPS stimulated DNA binding activity and the transcription activity of nuclear factor-kappa B (NF-kappaB)
Graham et al., J Immunol 2009 : Examination of TRAF binding, degradation, cytokine production, IgM secretion, and the activation of c-Jun kinase and NF-kappaB revealed that some events are dictated by TRAF binding site sequences, others are partially regulated, and still others are independent of the TRAF binding site sequence
Zuo et al., Arterioscler Thromb Vasc Biol 2009 (Atherosclerosis...) : Our findings show that mild renal dysfunction perturbs macrophage lipid homeostasis by inhibiting cholesterol efflux, mediated by decreased ABCA1 transporter and activation of NF-kappaB , and that ARB can restore cholesterol efflux
Akanji et al., Inflamm Res 2010 : Determine the effect of IL-1beta and dynamic compression on NFkappaB activation and IkappaB-alpha gene expression in chondrocyte/agarose constructs
Murtaza et al., Int J Cancer 2009 (Neoplasm Invasiveness...) : A cDNA array analysis revealed that fisetin modulates expression of more than 20 genes at transcription level with maximum decrease observed in DR3 expression and a parallel increase observed in the expression levels of IkappaBalpha , an NF-kappaB inhibitor
Bendinelli et al., Mol Cancer Res 2009 (Bone Neoplasms...) : NF-kappaB activation, dependent on acetylation/deacetylation, contributes to HIF-1 activity and migration of bone metastatic breast carcinoma cells
Nolan et al., PloS one 2009 (Sepsis) : In vitro, activation of macrophages with neutrophil lipid rafts caused selective disassociation of IRAK-M , a negative regulator of NF-kappaB signaling from CD80 ; providing a mechanism for preferential regulation of cytokine production by CD80
Montecucco et al., J Biomed Biotechnol 2009 (Inflammation) : Oxaprozin pro-apoptotic activity was associated with the inhibition of CD40L triggered Akt and NF-kappaB phosphorylation and the activation of caspase 3
Wu et al., Glia 2010 (Gliosis) : LPS triggered upregulation of CHL1 expression mediated through translocation of nuclear factor kappaB (NF-kappaB) to the nucleus is blocked by a specific NF-kappaB inhibitor and by inhibition of PI3K, PKCdelta, and ERK1/2 activities, implicating NF-kappaB as a downstream target for upregulation of CHL1 expression ... LPS triggered upregulation of CHL1 expression mediated through translocation of nuclear factor kappaB (NF-kappaB) to the nucleus is blocked by a specific NF-kappaB inhibitor and by inhibition of PI3K , PKCdelta, and ERK1/2 activities, implicating NF-kappaB as a downstream target for upregulation of CHL1 expression ... LPS triggered upregulation of CHL1 expression mediated through translocation of nuclear factor kappaB (NF-kappaB) to the nucleus is blocked by a specific NF-kappaB inhibitor and by inhibition of PI3K, PKCdelta, and ERK1/2 activities, implicating NF-kappaB as a downstream target for upregulation of CHL1 expression
Xia et al., Glia 2010 : IL-1beta stimulated IkappaBalpha degradation, NF-kappaB nuclear translocation, and transactivation in astrocytes ... In addition, overexpression of dominant negative IkappaBalpha in astrocytes inhibited IkappaBalpha degradation and NF-kappaB transactivation, and also inhibited the enhanced antibacterial effect induced by IL-1beta
Loiarro et al., J Biol Chem 2009 : Moreover, overexpression of a green fluorescent protein ( GFP ) -tagged mini-MyD88 protein ( GFP-MyD88- ( 27-72 ) ), comprising the Glu ( 52 ) and Tyr ( 58 ) residues, interfered with recruitment of both IRAK1 and IRAK4 by MyD88 and suppressed NF-kappaB activation by the interleukin-1 receptor but not by the MyD88 independent TLR3 ... Moreover, overexpression of a green fluorescent protein ( GFP ) -tagged mini-MyD88 protein ( GFP-MyD88- ( 27-72 ) ), comprising the Glu ( 52 ) and Tyr ( 58 ) residues, interfered with recruitment of both IRAK1 and IRAK4 by MyD88 and suppressed NF-kappaB activation by the interleukin-1 receptor but not by the MyD88 independent TLR3 ... Moreover, overexpression of a green fluorescent protein ( GFP ) -tagged mini-MyD88 protein ( GFP-MyD88- ( 27-72 ) ), comprising the Glu ( 52 ) and Tyr ( 58 ) residues, interfered with recruitment of both IRAK1 and IRAK4 by MyD88 and suppressed NF-kappaB activation by the interleukin-1 receptor but not by the MyD88 independent TLR3 ... GFP-MyD88- ( 27-72 ) exerted its effect by titrating IRAK1 and suppressing IRAK1 dependent NF-kappaB activation
Nah et al., Rheumatol Int 2010 (Arthritis, Rheumatoid) : Electrophoretic mobility shift assay ( EMSA ) was performed to investigate EGF mediated DNA binding activity of nuclear factor-kappaB (NF-kappaB) ... The DNA binding activity of NF-kappaB was remarkably increased by EGF treatment and the pretreatment of PD098059 abolished EGF stimulated NF-kappaB activity
Ang et al., J Cell Physiol 2009 (Bone Resorption) : Caffeic acid phenethyl ester, an active component of honeybee propolis attenuates osteoclastogenesis and bone resorption via the suppression of RANKL induced NF-kappaB and NFAT activity ... CAPE inhibited both constitutive and RANKL induced NF-kappaB and NFAT activation, concomitant with delayed IkappaBalpha degradation and inhibition of p65 nuclear translocation
de Mejia et al., Peptides 2009 (Inflammation) : The 5 kDa peptide inhibited most potently pro-inflammatory markers including interleukin-6 production ( IC ( 50 ) =2 microM ), interleukin-1beta production ( IC ( 50 ) =13 microM ), nuclear factor-kappa B (NF-kappaB) transactivation ( IC ( 50 ) =21 microM ), cyclooxygenase-2 expression ( IC ( 50 ) =25 microM ), nitric oxide production ( IC ( 50 ) =28 microM ), inducible nitric oxide synthase expression ( IC ( 50 ) =37 microM ), prostaglandin E ( 2 ) production ( IC ( 50 ) =41 microM ), p65 nuclear translocation ( IC ( 50 ) =48 microM ) and p50 nuclear translocation ( IC ( 50 ) =77 microM ) ... The 5 kDa peptide inhibited most potently pro-inflammatory markers including interleukin-6 production ( IC ( 50 ) =2 microM ), interleukin-1beta production ( IC ( 50 ) =13 microM ), nuclear factor-kappa B (NF-kappaB) transactivation ( IC ( 50 ) =21 microM ), cyclooxygenase-2 expression ( IC ( 50 ) =25 microM ), nitric oxide production ( IC ( 50 ) =28 microM ), inducible nitric oxide synthase expression ( IC ( 50 ) =37 microM ), prostaglandin E ( 2 ) production ( IC ( 50 ) =41 microM ), p65 nuclear translocation ( IC ( 50 ) =48 microM ) and p50 nuclear translocation ( IC ( 50 ) =77 microM ) ... The 5 kDa peptide inhibited most potently pro-inflammatory markers including interleukin-6 production ( IC ( 50 ) =2 microM ), interleukin-1beta production ( IC ( 50 ) =13 microM ), nuclear factor-kappa B (NF-kappaB) transactivation ( IC ( 50 ) =21 microM ), cyclooxygenase-2 expression ( IC ( 50 ) =25 microM ), nitric oxide production ( IC ( 50 ) =28 microM ), inducible nitric oxide synthase expression ( IC ( 50 ) =37 microM ), prostaglandin E ( 2 ) production ( IC ( 50 ) =41 microM ), p65 nuclear translocation ( IC ( 50 ) =48 microM ) and p50 nuclear translocation ( IC ( 50 ) =77 microM ) ... The 5 kDa peptide inhibited most potently pro-inflammatory markers including interleukin-6 production ( IC ( 50 ) =2 microM ), interleukin-1beta production ( IC ( 50 ) =13 microM ), nuclear factor-kappa B (NF-kappaB) transactivation ( IC ( 50 ) =21 microM ), cyclooxygenase-2 expression ( IC ( 50 ) =25 microM ), nitric oxide production ( IC ( 50 ) =28 microM ), inducible nitric oxide synthase expression ( IC ( 50 ) =37 microM ), prostaglandin E ( 2 ) production ( IC ( 50 ) =41 microM ), p65 nuclear translocation ( IC ( 50 ) =48 microM ) and p50 nuclear translocation ( IC ( 50 ) =77 microM ) ... The 5 kDa peptide inhibited most potently pro-inflammatory markers including interleukin-6 production ( IC ( 50 ) =2 microM ), interleukin-1beta production ( IC ( 50 ) =13 microM ), nuclear factor-kappa B (NF-kappaB) transactivation ( IC ( 50 ) =21 microM ), cyclooxygenase-2 expression ( IC ( 50 ) =25 microM ), nitric oxide production ( IC ( 50 ) =28 microM ), inducible nitric oxide synthase expression ( IC ( 50 ) =37 microM ), prostaglandin E ( 2 ) production ( IC ( 50 ) =41 microM ), p65 nuclear translocation ( IC ( 50 ) =48 microM ) and p50 nuclear translocation ( IC ( 50 ) =77 microM )
Zhang et al., Wei Sheng Yan Jiu 2009 : The results suggested LPS might activate NF-kappaB in peritoneal macrophages and induce the increase of transcription and expression of TNF-alpha , IL-1beta, IL-6 genes ; Res could inhibit the activation of NF-kappaB and regulate the expression of TNF-alpha, IL-1beta and IL-6 genes in peritoneal macrophages ... The results suggested LPS might activate NF-kappaB in peritoneal macrophages and induce the increase of transcription and expression of TNF-alpha, IL-1beta, IL-6 genes ; Res could inhibit the activation of NF-kappaB and regulate the expression of TNF-alpha, IL-1beta and IL-6 genes in peritoneal macrophages
Feng et al., Inflamm Res 2010 : Further study showed that lycopene also inhibited LPS induced IkappaB phosphorylation, IkappaB degradation, and NF-kappaB translocation
Yin et al., Mol Cell Biochem 2010 : Two mechanistically distinct inhibitors of NF-kappaB signaling, sulfasalazine ( NF-kappaB inhibitor ) and WY-14643 [ PPAR alpha ( peroxisome proliferator activated receptor alpha ) agonist ] that interfere with the transcription factor RELA ( p65 ), suppressed TNF-alpha induced RIP-2 gene expression, which indicated that NF-kappaB signaling was involved in the cytokine induced transcriptional activation of RIP-2 gene expression ... NF-kappaB mediated regulation of RIP-2 gene and protein expression suggests an additional step in the regulation of NF-kappaB function as RIP-2 has been shown to positively modulate NF-kappaB by binding IKK gamma ( I kappaB kinase gamma ), a component of the IKK complex ... These findings support a positive feed-forward mechanism of NF-kappaB regulation that involves NF-kappaB dependent induction of RIP-2 transcription and a subsequent increase in RIP-2 protein levels in response to inflammatory cytokines
Thylur et al., J Cell Biochem 2009 (Calcium Signaling...) : The ectopic expression of swiprosin-1 augmented PMA/A23187 induced NF-kappaB promoter activity, and resulted in increased expression of cytokines
Ogasawara et al., J Cell Biochem 2009 : Hemoglobin induces the expression of indoleamine 2,3-dioxygenase in dendritic cells through the activation of PI3K, PKC, and NF-kappaB and the generation of reactive oxygen species ... Hb-induced IDO expression was inhibited by inhibitors of PI3-kinase (PI3K), PKC and nuclear factor (NF)-kappaB ... These results suggest that the activation of NF-kappaB through the PI3K-PKC-ROS and PI3K-Akt pathways is required for the Hb-induced IDO expression in BMDCs
Zhang et al., Br J Pharmacol 2009 : Moreover, Ucn2 activated p38MAPK and augmented NF-kappaB nuclear translocation and phosphorylation, whereas ERK1/2, JNK and Akt pathways were not involved in this process ... These findings suggest that Ucn1 exerts pro-inflammatory effects by augmenting LPS induced expression of COX-2 and ICAM-1 in RAECs via CRF ( 2 ) receptors and the activation of p38MAPK and NF-kappaB ... These findings suggest that Ucn1 exerts pro-inflammatory effects by augmenting LPS induced expression of COX-2 and ICAM-1 in RAECs via CRF ( 2 ) receptors and the activation of p38MAPK and NF-kappaB ... These findings suggest that Ucn1 exerts pro-inflammatory effects by augmenting LPS induced expression of COX-2 and ICAM-1 in RAECs via CRF ( 2 ) receptors and the activation of p38MAPK and NF-kappaB ... These findings suggest that Ucn1 exerts pro-inflammatory effects by augmenting LPS induced expression of COX-2 and ICAM-1 in RAECs via CRF ( 2 ) receptors and the activation of p38MAPK and NF-kappaB
Cohen et al., Biochem Biophys Res Commun 2009 : ABIN-1 negatively regulates NF-kappaB by inhibiting processing of the p105 precursor ... p105 plays dual roles in NF-kappaB signaling : in its precursor form it inhibits NF-kappaB activation, but limited processing by the ubiquitin system generates the p50 active subunit of the transcription factor
Vanhara et al., Differentiation 2009 (Prostatic Neoplasms) : GDF-15 inhibits expression of c-Fos and activity of NFkappaB by delayed degradation of IkappaB
Romano et al., Cell Death Differ 2010 (Melanoma) : FKBP51 was required for the activation of Rx-induced NF-kappaB , which in turn inhibited apoptosis by stimulating X-linked inhibitor of apoptosis protein and promoting authophagy mediated Bax degradation
Eder et al., Biochem Biophys Res Commun 2009 : The most ancient part, lipid A is crucial in evoking immediate TNF release and activation of NF-kappaB
Oh et al., Neurosci Lett 2009 : We showed that OA blocked LPS induced NF-kappaB activation and phosphorylation of inhibitor kappaB kinase (IKK)
Ndisang et al., J Card Fail 2009 (Hypertension...) : Aldosterone and phospholipase C (PLC) stimulate nuclear factor-kappaB (NF-kappaB) and activating-protein ( AP-1 ), causing fibrosis and hypertrophy
Brenner et al., Proc Natl Acad Sci U S A 2009 : Phosphorylation of CARMA1 by HPK1 is critical for NF-kappaB activation in T cells ... Knock-down of HPK1 abrogates TCR induced IKKbeta and NF-kappaB activation, whereas active HPK1 leads to increased IKKbeta activity in T cells ... Here, we show that HPK1 mediated NF-kappaB activation is dependent on the adaptor protein CARMA1
Fan et al., J Biol Chem 2009 : Here we report that tumor necrosis factor-alpha (TNFalpha) induces RelA polyubiquitination at the lysine 195 residue, and this ubiquitination event is critical for the degradation of RelA and termination of TNFalpha mediated NF-kappaB activation ... Overexpression of a RelA mutant with an arginine substitution for the lysine 195 residue dramatically inhibits RelA polyubiquitination and induces a stronger NF-kappaB activation compared with the wild type ... Our finding is the first report that substitution of a key RelA lysine residue with arginine inhibits TNFalpha induced RelA ubiquitination and enhances TNFalpha induced NF-kappaB activation
Ahmad et al., Cancer Res 2009 : Studies in nonmalignant epithelial cells show that MUC1-C interacts with NF-kappaB in the response to tumor necrosis factor-alpha stimulation
Welch et al., Cancer Res 2009 (Neoplasms) : Our results reveal a novel proapoptotic factor that is induced by TNF-alpha through NF-kappaB and that interacts with and antagonizes Bcl-2
Raices et al., PloS one 2009 : NFkappaB inhibition resulted in decreased IL-1beta/IL-18/TNFalpha stimulated IFNgamma release ... Overall, our data suggests that IkappaBzeta positively regulates NFkappaB mediated IFNgamma production in KG-1 cells
Nagy et al., Molecular cancer 2009 (Lymphoma) : Moreover, depletion of STAT5 via selective antisense oligonucleotide treatment similarly resulted in decreased BCL10 mRNA and protein expression, cellular viability and impaired NFkappaB activity independent of IL-2
Dommisch et al., Innate Immun 2010 : Phospholipase C, p38/MAPK, and NF-kappaB mediated induction of MIP-3alpha/CCL20 by Porphyromonas gingivalis
Theiss et al., Mol Biol Cell 2009 (Adenocarcinoma...) : Restoration of importin alpha3 levels sustained NF-kappaB activation by TNF-alpha during PHB transfection ... These results suggest that PHB inhibits NF-kappaB nuclear translocation via a novel mechanism involving alteration of importin alpha3 levels ... Sustained expression of prohibitin in intestinal epithelial cells in vitro and in vivo ( prohibitin transgenic mice, PHB TG ) resulted in a marked decrease in TNF-alpha induced nuclear translocation of the NF-kappaB protein p65, NF-kappaB/DNA binding, and NF-kappaB mediated transcriptional activation despite robust IkappaB-alpha phosphorylation and degradation and increased cytosolic p65
Park et al., Mol Cells 2009 : Hepatitis delta virus large antigen sensitizes to TNF-alpha induced NF-kappaB signaling ... In this study, we demonstrated that TNF-alpha induced NF-kappaB transcriptional activation was increased by LHDAg but not by SHDAg in both HEK293 and Huh7 cells ... Furthermore, LHDAg promoted TRAF2 induced NF-kappaB activation
Kajiya et al., J Cell Physiol 2009 (Necrosis) : BDNF enhanced phosphorylated Akt levels, NF-kappaB activity in the nucleus, Bcl-2 mRNA levels, and mitochondrial membrane potential
Konda et al., Journal of inflammation (London, England) 2009 : In addition, RIAA inhibited NF-kappaB mediated inflammatory markers in various cell models, including nitric oxide in LPS stimulated RAW 264.7 cells, RANKL mediated TRAP activity in transformed osteoclasts, and TNF-alpha/IL-1beta mediated MMP-13 expression in SW1353 human chondrosarcoma cells ... In addition, RIAA inhibited NF-kappaB mediated inflammatory markers in various cell models, including nitric oxide in LPS stimulated RAW 264.7 cells, RANKL mediated TRAP activity in transformed osteoclasts, and TNF-alpha/IL-1beta mediated MMP-13 expression in SW1353 human chondrosarcoma cells
Calegari-Silva et al., Immunol Lett 2009 (Leishmaniasis) : NF-kappaB mediated repression of iNOS expression in Leishmania amazonensis macrophage infection
Ozer et al., Cancer Biol Ther 2009 (Breast Neoplasms...) : NUP inhibited both inducible and constitutive NFkappaB activation and affected the canonical and alternative pathways
Meusch et al., Arthritis Rheum 2009 (Arthritis, Rheumatoid) : Instead, transmembrane TNF reverse signaling inhibited the constitutive NF-kappaB activation in RA monocytes, suppressed IL-1beta secretion, and normalized spontaneous in vitro apoptosis
Borthakur et al., Inflamm Bowel Dis 2010 : siRNA silencing of Bcl10 was used to examine its role in PAF induced NF-kappaB activation and IL-8 production ... Bcl10 was required for PAF induced I kappaB alpha phosphorylation, NF-kappaB activation, and IL-8 production in NCM460, a cell line derived from normal human colon, and Caco-2, a transformed human intestinal cell line ... Bcl10 was required for PAF induced I kappaB alpha phosphorylation, NF-kappaB activation, and IL-8 production in NCM460, a cell line derived from normal human colon, and Caco-2, a transformed human intestinal cell line
Ghadimi et al., Inflamm Bowel Dis 2010 : In response to apically applied natural commensal-origin DNA, polarized HT-29 and T84 cells enhanced expression of TLR9 in a specific manner, which was subsequently associated with attenuation of TNF-alpha induced NF-kappaB activation and NF-kappaB mediated IL-8 expression ... In response to apically applied natural commensal-origin DNA, polarized HT-29 and T84 cells enhanced expression of TLR9 in a specific manner, which was subsequently associated with attenuation of TNF-alpha induced NF-kappaB activation and NF-kappaB mediated IL-8 expression
Rath et al., DNA Cell Biol 2009 (Lung Neoplasms) : We show that expression of wt-p53 from a recombinant adenovirus-p53 followed by treatment with 2-methoxyestradiol ( 2-ME ), an endogenous, nontoxic, estrogenic metabolite, resulted in differential NF-kappaB activation and inhibitor kappaB alpha ( IkappaB-alpha ) degradation in three different human lung cancer cell lines with different p53 phenotypes ... In contrast, either adeno-p53 expression or 2-ME treatment induced NF-kappaB activation in the p53 deleted H1299 cells, but H460 cells, containing wt-p53, did not show NF-kappaB activation under any of these conditions
Zhou et al., Exp Neurol 2009 (Disease Models, Animal...) : We have previously reported that the anti-inflammatory cytokine IL-10 induces a number of signaling cascades through the IL-10 receptor in spinal cord neurons in vitro to activate NF-kappaB transcription Bcl-2 and Bcl-x ( L ) and that, after exposure to glutamate IL-10, blocks cytochrome c release and caspase cleavage
Lee et al., Biochim Biophys Acta 2009 : DSCR1-1S also stimulated IL-1R mediated signaling pathways, TAK1 activation, NF-kappaB transactivation , and IL-8 production, all downstream consequences of IL-1R activation ... DSCR1-1S also stimulated IL-1R mediated signaling pathways, TAK1 activation, NF-kappaB transactivation , and IL-8 production, all downstream consequences of IL-1R activation ... DSCR1-1S also stimulated IL-1R mediated signaling pathways, TAK1 activation, NF-kappaB transactivation, and IL-8 production, all downstream consequences of IL-1R activation ... DSCR1-1S also stimulated IL-1R mediated signaling pathways, TAK1 activation, NF-kappaB transactivation, and IL-8 production, all downstream consequences of IL-1R activation
Chang et al., Pharmacol Res 2009 : In this study, we further investigated the role of Raf-1 in YC-1 induced nuclear factor-kappaB (NF-kappaB) activation and COX-2 expression in A549 cells
Kenny et al., J Immunol 2009 : Mal was not required for the induction of IL-6 or NFkappaB activation at high concentrations of the TLR1/2 ligand Pam ( 3 ) Cys-Ser- ( Lys ) ( 4 ) or the TLR2/6 ligand macrophage activating lipopeptide-2 and was required for these responses only at low ligand concentrations
Liu et al., Proc Natl Acad Sci U S A 2009 (Inflammation) : Expression of miR-147 was greater after cellular activation by TLR4 than after engagement of either TLR2 or TLR3, suggesting that maximal induction of miR-147 required activation of both NF-kappaB and IRF3
Moharregh-Khiabani et al., Current neuropharmacology 2009 : In vitro studies with the ester dimethyl fumarate ( DMF ) described an inhibitory effect on nuclear factor kappa B (NF-kappaB) dependent transcription of tumor necrosis factor-alpha (TNF-alpha) induced genes in human endothelial cells
Cristofanon et al., Ann N Y Acad Sci 2009 : ( 1 ) Here we show that melatonin induces a strong canonical activation of NF-kappaB , inducing IkappaBalpha degradation and the consequential nuclear translocation of p50/p65 subunits ... ( 1 ) Here we show that melatonin induces a strong canonical activation of NF-kappaB , inducing IkappaBalpha degradation and the consequential nuclear translocation of p50/p65 subunits
Weng et al., J Biomed Sci 2009 (Liver Cirrhosis, Biliary...) : Arm also suppressed TNF-alpha induced collagen collagen deposition, NFkappaB activation and MAPK ( p38 , ERK1/2, and JNK ) phosphorylations ... Arm also suppressed TNF-alpha induced collagen collagen deposition, NFkappaB activation and MAPK ( p38, ERK1/2 , and JNK ) phosphorylations
Tsukamoto et al., Clin Cancer Res 2009 : Additionally, NF-kappaB and mammalian target of rapamycin are involved in regulating ILT7L expression
Han et al., Korean J Parasitol 2009 (Trichomonas Infections) : However, trichomonad induced NF-kappaB activation and TNF-alpha production in macrophages were significantly inhibited by inhibition of iNOS levels with L-NMMA ( NO synthase inhibitor )
Ye et al., Nan Fang Yi Ke Da Xue Xue Bao 2009 : TNF-alpha induced the activation of NF-kappaB and increased the expressions of IL-6 and sICAM-1 in HUVECs
Huang et al., J Clin Microbiol 2009 : The assay readout is the induction of NF-kappaB and/or cytokines in response to TLR activation
Romacho et al., Diabetologia 2009 (Diabetes Mellitus...) : By using the respective ERK 1/2 and NF-kappaB inhibitors, PD98059 and pyrrolidine dithiocarbamate, we established that iNOS induction by ePBEF/NAMPT/visfatin required the consecutive upstream activation of ERK 1/2 and NF-kappaB ... By using the respective ERK 1/2 and NF-kappaB inhibitors, PD98059 and pyrrolidine dithiocarbamate, we established that iNOS induction by ePBEF/NAMPT/visfatin required the consecutive upstream activation of ERK 1/2 and NF-kappaB ... However, exogenous nicotinamide mononucleotide, the product of NAMPT activity, mimicked NF-kappaB activation and iNOS induction by ePBEF/NAMPT/visfatin, while the NAMPT inhibitor APO866 prevented the effects of ePBEF/NAMPT/visfatin on iNOS and NF-kappaB ... However, exogenous nicotinamide mononucleotide, the product of NAMPT activity, mimicked NF-kappaB activation and iNOS induction by ePBEF/NAMPT/visfatin, while the NAMPT inhibitor APO866 prevented the effects of ePBEF/NAMPT/visfatin on iNOS and NF-kappaB ... However, exogenous nicotinamide mononucleotide, the product of NAMPT activity, mimicked NF-kappaB activation and iNOS induction by ePBEF/NAMPT/visfatin, while the NAMPT inhibitor APO866 prevented the effects of ePBEF/NAMPT/visfatin on iNOS and NF-kappaB
Jackman et al., Exp Cell Res 2009 : We have investigated a well studied signaling pathway, TNF-alpha induced NF-kappaB activation, and found a connection between the stability of microtubules and the regulation of NF-kappaB signaling in C2C12 myotubes ... Although there was no additive effect of taxol and TNF-alpha on NF-kappaB activity suggesting a shared mechanism of activation, taxol strongly induced the NF-kappaB reporter in the presence of a TNF receptor (TNFR) blocking antibody while TNF-alpha did not ... Both TNF-alpha and taxol induce the degradation of endogenous IkappaBalpha and either taxol or TNF-alpha induction of NF-kappaB activity was blocked by inhibitors of NF-kappaB acting at different sites in the signaling pathway ... Both TNF-alpha and taxol induce the degradation of endogenous IkappaBalpha and either taxol or TNF-alpha induction of NF-kappaB activity was blocked by inhibitors of NF-kappaB acting at different sites in the signaling pathway ... Both TNF-alpha and taxol strongly induce known NF-kappaB chemokine target genes ... On the other hand, if microtubules are destabilized by colchicine, then the induction of NF-kappaB by TNF-alpha or taxol is greatly reduced
Cappello et al., Cell Signal 2009 : This was accompanied by elevated nuclear p65 and NF-kappaB activity in the presence of C/EBPbeta
Choi et al., Atherosclerosis 2010 : This study suggests that GSA induces expression of IL-8 in VSMCs and that TLR-4, mitogen activated protein kinases, NF-kappaB , and NADPH oxidase are involved in that process
Degagné et al., J Immunol 2009 : P2Y2 receptor transcription is increased by NF-kappa B and stimulates cyclooxygenase-2 expression and PGE2 released by intestinal epithelial cells ... P2Y2 receptor transcription is increased by NF-kappa B and stimulates cyclooxygenase-2 expression and PGE2 released by intestinal epithelial cells ... P2Y2 receptor transcription is increased by NF-kappa B and stimulates cyclooxygenase-2 expression and PGE2 released by intestinal epithelial cells
Li et al., J Dent Res 2009 : NF-kappaB activation was noted during interaction of epithelial cells with C. glabrata, and pre-treatment with an NF-kappaB inhibitor partly inhibited GM-CSF synthesis
Chang et al., Nat Immunol 2009 (Inflammation) : Peli1 was required for TLR3 induced activation of IkappaB kinase (IKK) and its ` downstream ' target, transcription factor NF-kappaB , but was dispensable for IKK-NF-kappaB activation induced by several other TLRs and the interleukin 1 (IL-1) receptor ... Peli1 was required for TLR3 induced activation of IkappaB kinase (IKK) and its ` downstream ' target, transcription factor NF-kappaB, but was dispensable for IKK-NF-kappaB activation induced by several other TLRs and the interleukin 1 (IL-1) receptor ... Peli1 was required for TLR3 induced activation of IkappaB kinase (IKK) and its ` downstream ' target, transcription factor NF-kappaB, but was dispensable for IKK-NF-kappaB activation induced by several other TLRs and the interleukin 1 (IL-1) receptor ... Peli1 was required for TLR3 induced activation of IkappaB kinase (IKK) and its ` downstream ' target, transcription factor NF-kappaB , but was dispensable for IKK-NF-kappaB activation induced by several other TLRs and the interleukin 1 (IL-1) receptor
Kairisalo et al., Eur J Neurosci 2009 : NF-kappaB dependent regulation of brain derived neurotrophic factor in hippocampal neurons by X-linked inhibitor of apoptosis protein ... BDNF itself activated NF-kappaB in the neurons at higher concentrations
Anand et al., Biochem Pharmacol 2010 : When examined by electrophoretic gel shift mobility assay, curcumin ( NP ) was more active than curcumin in inhibiting TNF induced NF-kappaB activation and in suppression of NF-kappaB regulated proteins involved in cell proliferation ( cyclin D1 ), invasion ( MMP-9 ), and angiogenesis ( VEGF )
Bruno et al., Gut 2010 (HIV Infections...) : gp120 also induced activation of nuclear factor-kappaB (NF-kappaB) and p38 ( MAPK )
Dai et al., Cell Res 2009 (Carcinoma, Hepatocellular...) : We also demonstrated that p28(GANK) increases p38 mitogen activated protein kinase and Akt phosphorylation, and inhibits nuclear factor kappa B (NF-kappaB) activation under ER stress, which in turn contributes to GRP78 upregulation
Liu et al., Microb Pathog 2010 : Deletion of Braun lipoprotein gene ( lpp ) attenuates Yersinia pestis KIM/D27 strain : role of Lpp in modulating host immune response, NF-kappaB activation and cell death
Morgan et al., Mol Cell Biol 2009 : However, this region contains NF-kappaB binding sites, and as shown here, NF-kappaB signaling is required for Prdm1 induction
Liang et al., J Biol Chem 2009 : We report here that TTP also negatively regulates NF-kappaB signaling at the transcriptional corepressor level, by which it may repress inflammatory gene transcription
Bai et al., J Cell Biochem 2009 (Liver Neoplasms...) : Based on our previous observations that the nutrient flavonoid luteolin potently blocks TNF induced NF-kappaB activation in cancer cells, we investigated if the combination of SMC3 and luteolin would achieve a synergistic anticancer activity ... The results show that luteolin had no effect on autocrine TNF but it effectively blocked SMC3 induced nuclear factor kappa B (NF-kappaB) activation and expression of anti-apoptotic NF-kappaB targets
Rydberg et al., Immunology 2009 (Carcinoma, Squamous Cell...) : In Detroit-562, TLR2 and TLR5 activation was mediated via c-jun N-terminal kinase ( JNK ) -, p38-, phosphatidylinositol 3-kinase (PI3K)- and nuclear factor (NF)-kappaB related pathways, while TLR3 was dependent on NF-kappaB
Khuda et al., Immunology 2009 : AEG-1 induced by LPS subsequently regulated NF-kappaB activation
Kuo et al., Mol Nutr Food Res 2009 : The repression of iNOS but not COX-2 expression may come from the differential effect of inotilone on nuclear factor-kappaB (NFkappaB) and CCAAT/enhancer binding protein beta Treatment with inotilone resulted in the reduction of LPS induced nuclear translocation of NFkappaB subunit and the NFkappaB dependent transcriptional activity by blocking phosphorylation of inhibitor kappaB(IkappaB)alpha and p65 and subsequent degradation of inhibitor kappaBalpha
Connelly et al., J Cell Physiol 2010 (Disease Models, Animal...) : We found that activation of NF-kappaB during involution resulted in a more rapid reduction in milk levels and increased cleavage of caspase-3 , an indicator of apoptosis
García et al., Biochim Biophys Acta 2009 (Enterovirus Infections) : PICin induced NF-kappaB and IFN-beta promoter activation were prevented by the DN-RIG-I
Wang et al., Cytokine 2009 (Acute Lung Injury) : ZD 7155 also reduced the mRNA expression of TNF-alpha and IL-1 beta, inhibited the activation of NF-kappaB and AP-1, and improved lung histopathology ... ZD 7155 also reduced the mRNA expression of TNF-alpha and IL-1 beta , inhibited the activation of NF-kappaB and AP-1, and improved lung histopathology
Taylor et al., Ann N Y Acad Sci 2009 (Hantavirus Pulmonary Syndrome...) : Inhibition of TNF-alpha induced activation of NF-kappaB by hantavirus nucleocapsid proteins ... We recently reported that the nucleocapsid ( N ) protein of HFRS causing Hantaan virus ( HTNV ) inhibits tumor necrosis factor-alpha (TNF-alpha) activation of nuclear factor-kappaB (NF-kappaB) ... We found that like HTNV N, the N proteins of HFRS causing Seoul and Dobrava viruses inhibited TNF-alpha activation of NF-kappaB and translocation of the NF-kappaB p65 subunit, but did not interfere with degradation of inhibitor of NF-kappaB ( IkappaB ) ... In contrast, the HFRS causing Puumala virus and the HPS causing Andes and Sin Nombre viruses did not prevent TNF-alpha activation of NF-kappaB or nuclear translocation of p65
Kai et al., FEBS Lett 2009 : We recently reported that diacylglycerol kinase (DGK) alpha enhanced tumor necrosis factor-alpha (TNF-alpha) induced activation of nuclear factor-kappaB (NF-kappaB) ... We recently reported that diacylglycerol kinase (DGK) alpha enhanced tumor necrosis factor-alpha (TNF-alpha) induced activation of nuclear factor-kappaB (NF-kappaB) ... Moreover, knockdown and overexpression of PKCzeta suppressed and synergistically enhanced DGKalpha mediated NF-kappaB activation, respectively ... These results strongly suggest that DGKalpha positively regulates TNF-alpha dependent NF-kappaB activation via the PKCzeta mediated Ser311 phosphorylation of p65/RelA ... These results strongly suggest that DGKalpha positively regulates TNF-alpha dependent NF-kappaB activation via the PKCzeta mediated Ser311 phosphorylation of p65/RelA
Yadav et al., J Immunol 2009 (Asthma) : We observed that pharmacological inhibition or genetic ablation of AR by small interfering RNA prevented TNF-alpha- as well as LPS induced apoptosis ; reactive oxygen species generation ; synthesis of inflammatory markers IL-6, IL-8, and PGE ( 2 ) ; and activation of NF-kappaB and AP-1 in small airway epithelial cells
Yuan et al., J Immunol 2009 : Amphioxus TRAF1/2 and 3 molecules displayed various expression patterns in response to microbial infection, and some of them can attenuate the NF-kappaB activation mediated by human TRAF2 and 6 ... Amphioxus TRAF1/2 and 3 molecules displayed various expression patterns in response to microbial infection, and some of them can attenuate the NF-kappaB activation mediated by human TRAF2 and 6 ... Amphioxus TRAF1/2 and 3 molecules displayed various expression patterns in response to microbial infection, and some of them can attenuate the NF-kappaB activation mediated by human TRAF2 and 6
Wang et al., Mol Biol Rep 2010 : A possible effect of interaction between PhiC31 integrase and TTRAP was highlighted by the fact that PhiC31 integrase inhibited the NFkappaB activation mediated by IL-1 in a dose dependent manner
Husvik et al., Eur J Oral Sci 2009 (Carcinoma, Basosquamous...) : Neither the transcription factor nuclear factor-kappaB (NF-kappaB) , nor Src, was involved in EGF induced COX-2 expression
Lee et al., Exp Dermatol 2010 : In addition, NF-kappaB or JNK mediated the MMP-1 expression induced by TLR2, TLR3 and TLR5, whereas NF-kappaB, JNK or p38 MAPK mediated the MMP-9 expression induced by TLR2, TLR3 and TLR5 ... In addition, NF-kappaB or JNK mediated the MMP-1 expression induced by TLR2, TLR3 and TLR5, whereas NF-kappaB , JNK or p38 MAPK mediated the MMP-9 expression induced by TLR2, TLR3 and TLR5
Lee et al., J Mol Med (Berl) 2009 (Edema...) : BITC inhibited LPS induced phosphorylation and the degradation of the inhibitor of kappaBalpha, translocation of p65 into the nucleus, and the DNA binding activity and transcriptional activity of NFkappaB
Rivas et al., Breast Cancer Res Treat 2010 (Breast Neoplasms...) : Moreover, TNFalpha promoted ErbB-2/ErbB-3 heterocomplex formation, Akt activation and NF-kappaB transcriptional activation ... Inhibition of ErbB-2 by addition of AG825, an epidermal growth factor receptor/ErbB-2-tyrosine kinase inhibitor, or knockdown of ErbB-2 by RNA interference strategy, blocked TNFalpha induced NF-kappaB activation and proliferation ... Inhibition of ErbB-2 by addition of AG825, an epidermal growth factor receptor/ErbB-2-tyrosine kinase inhibitor, or knockdown of ErbB-2 by RNA interference strategy, blocked TNFalpha induced NF-kappaB activation and proliferation
Shoji et al., Retrovirology 2009 (Cell Transformation, Viral...) : Using a series of Tax1 chimeric proteins with Tax2, we found that amino acids 225-232 of Tax1 , the Tax1 ( 225-232 ) region, was essential for the activation of NF-kappa B2 as well as for the high transforming activity
Gavaldà et al., Development 2009 : Shortly before birth, BDNF activates NF-kappaB by an atypical mechanism that involves tyrosine phosphorylation of IkappaBalpha by Src family kinases, and dephosphorylates p65 at serine 536 ... Immediately after birth, BDNF independent constitutive activation of NF-kappaB signalling by serine phosphorylation of IkappaBalpha and constitutive dephosphorylation of p65 at serine 536 are required for BDNF promoted neurite growth
Takeda et al., Arterioscler Thromb Vasc Biol 2009 : DMOG rather suppressed LPS induced NF-kappaB transcriptional activity
Hsieh et al., Neurotox Res 2010 : Taken together, these results suggested that in RBA-1 cells, activation of NF-kappaB by a c-Src dependent PI3K/Akt-p42/p44 MAPK activation mediated through transactivation of PDGFR is essential for MMP-9 gene upregulation induced by LTA ... Taken together, these results suggested that in RBA-1 cells, activation of NF-kappaB by a c-Src dependent PI3K/Akt-p42/p44 MAPK activation mediated through transactivation of PDGFR is essential for MMP-9 gene upregulation induced by LTA ... Taken together, these results suggested that in RBA-1 cells, activation of NF-kappaB by a c-Src dependent PI3K/Akt-p42/p44 MAPK activation mediated through transactivation of PDGFR is essential for MMP-9 gene upregulation induced by LTA
Momeny et al., Med Oncol 2010 : Arsenic trioxide induces apoptosis in NB-4, an acute promyelocytic leukemia cell line, through up-regulation of p73 via suppression of nuclear factor kappa B-mediated inhibition of p73 transcription and prevention of NF-kappaB mediated induction of XIAP , cIAP2, BCL-XL and survivin ... Microculture tetrazolium test, bromodeoxyuridine ( BrdU ) cell proliferation assay, caspase 3 activity assay, cell based nuclear factor kappa B (NF-kappaB) phosphorylation measurement by ELISA and real-time RT-PCR were employed to appraise the effects of ATO on metabolic activity, DNA synthesis, induction of programmed cell death and NF-kappaB activation ... Altogether, these issues show for the first time that ATO treatment could trammel cell growth and proliferation as well as induces apoptosis in NB-4 cells through induction of transcriptional levels of p73, TP53INP1, WNK2, lipocalin 2 as well as suppression of NF-kappaB mediated induction of BCL-X ( L ), cIAP2 , XIAP and survivin ... Altogether, these issues show for the first time that ATO treatment could trammel cell growth and proliferation as well as induces apoptosis in NB-4 cells through induction of transcriptional levels of p73, TP53INP1, WNK2, lipocalin 2 as well as suppression of NF-kappaB mediated induction of BCL-X ( L ), cIAP2, XIAP and survivin
Shi et al., Sichuan Da Xue Xue Bao Yi Xue Ban 2009 : To investigate the expression of CTGF, CTGF mRNA, NF-kappaB and AP-1 induced by TGF-beta1 in human lung fibroblast ( HLF-02 ), and study the effect and possible mechanism of rosiglitazone on signal pathways of TGF-beta1 in HLF-02 ... The effects of TGF-beta1 , curcumin, PDTC and rosiglitazone on the expression of CTGF, NF-kappaB and AP-1 were evaluated with Western blot
Kim et al., Eur J Pharmacol 2009 (Inflammatory Bowel Diseases) : In addition, M1-M4 significantly suppressed TNF-alpha induced NF-kappaB transcriptional activity ... Treatment of HT-29 cells with M1 and PDTC, a NF-kappaB inhibitor, synergistically suppressed both TNF-alpha induced NF-kappaB activation and monocytic cell adhesion to HT-29 cells
Lim et al., Food Chem Toxicol 2010 : Curcumin inhibits phorbol myristate acetate ( PMA ) -induced MCP-1 expression by inhibiting ERK and NF-kappaB transcriptional activity
Kim et al., Toxicological sciences : an official journal of the Society of Toxicology 2009 : The MT-III induced antiapoptotic effects and increase in NF-kappaB activity were blocked by specific inhibitors of TrkA, phosphatidylinositol-3 kinase (PI3K), Akt, or NF-kappaB, indicating that MT-III provides neuronal protection by activating NF-kappaB through the TrkA/PI3K/Akt signaling pathway ... The MT-III induced antiapoptotic effects and increase in NF-kappaB activity were blocked by specific inhibitors of TrkA, phosphatidylinositol-3 kinase (PI3K), Akt , or NF-kappaB, indicating that MT-III provides neuronal protection by activating NF-kappaB through the TrkA/PI3K/Akt signaling pathway ... The MT-III induced antiapoptotic effects and increase in NF-kappaB activity were blocked by specific inhibitors of TrkA , phosphatidylinositol-3 kinase (PI3K), Akt, or NF-kappaB, indicating that MT-III provides neuronal protection by activating NF-kappaB through the TrkA/PI3K/Akt signaling pathway ... The MT-III induced antiapoptotic effects and increase in NF-kappaB activity were blocked by specific inhibitors of TrkA, phosphatidylinositol-3 kinase (PI3K) , Akt, or NF-kappaB, indicating that MT-III provides neuronal protection by activating NF-kappaB through the TrkA/PI3K/Akt signaling pathway
Chehl et al., HPB (Oxford) 2009 : The effect of Tq on the constitutive and TNF-alpha induced activation and nuclear translocation of NF-kappaB was examined by ELISA and immunohistochemistry ... Tq also inhibited the constitutive and TNF-alpha mediated activation of NF-kappaB in PDA cells and reduced the transport of NF-kappaB from the cytosol to the nucleus
Yao et al., J Clin Invest 2009 (Bone Resorption) : TRAF3 siRNA prevented TNF induced NF-kappaB p100 accumulation and inhibition of osteoclastogenesis ... These findings suggest that upregulation of TRAF3 or NF-kappaB p100 expression or inhibition of NF-kappaB p100 degradation in OCPs could limit bone destruction and inflammation induced bone loss in common bone diseases ... These findings suggest that upregulation of TRAF3 or NF-kappaB p100 expression or inhibition of NF-kappaB p100 degradation in OCPs could limit bone destruction and inflammation induced bone loss in common bone diseases
Zhang et al., Inflammation 2010 (Inflammation) : Induction of COX-2 by resistin was also markedly reduced in the presence of either dominant negative mutant IkappaBalpha or PDTC, a pharmacological inhibitor of NF-kappaB ... Induction of COX-2 by resistin was also markedly reduced in the presence of either dominant negative mutant IkappaBalpha or PDTC, a pharmacological inhibitor of NF-kappaB ... These results suggest that resistin enhances COX-2 expression in mouse macrophage cells in a TAK1-IKK-NF-kappaB dependent manner and therefore plays a critical role in inflammatory processes
Won et al., Planta Med 2010 : Furthermore, madecassic acid suppressed the LPS induced activation of nuclear factor-kappaB (NF-kappaB) , and this was associated with the abrogation of inhibitory kappa B-alpha ( IkappaB-alpha ) degradation and with the subsequent blocking of p65 protein translocation to the nucleus
Neves et al., Planta Med 2010 : Screening of five essential oils for identification of potential inhibitors of IL-1 induced Nf-kappaB activation and NO production in human chondrocytes : characterization of the inhibitory activity of alpha-pinene ... The ability of the alpha-pinene containing fraction to reduce IL-1 induced NF-kappaB activation and NO production warrants further studies to demonstrate the usefulness of alpha-pinene in the treatment of arthritic diseases and other conditions in which NF-kappaB and NO play pathological roles
Walker et al., Stem Cells Dev 2010 : Direct intrathecal implantation of mesenchymal stromal cells leads to enhanced neuroprotection via an NFkappaB mediated increase in interleukin-6 production
Togano et al., Biochem Biophys Res Commun 2009 (Neoplasms) : A new NF-kappaB inhibitor, dehydroxymethylepoxyquinomicin ( DHMEQ ), reduced viability and induced apoptosis in St-4/CPT , A549/CPT, and HT-29/CPT cell lines, while their parental cell lines were resistant to DHMEQ
Yang et al., Neurochem Int 2010 (Nerve Degeneration...) : We show that NF-kappaB was activated by MPP ( + ) as evidenced by NF-kappaB p65 nuclear translocation, the increased DNA binding activity and a rapid phosphorylation of NF-kappaB inhibitor ( IkappaBalpha )
Jamaluddin et al., Arterioscler Thromb Vasc Biol 2009 : Eotaxin also increased the phosphorylation of Stat3 and nuclear translocation of NF-kappaB in HCAECs
Lim et al., Am J Physiol Gastrointest Liver Physiol 2009 : TLR3 mediated NF-{kappa}B signaling in human esophageal epithelial cells ... Through reporter gene studies, we show that poly ( I:C ) -induced NF-kappaB activation is critical for the transactivation of the IL-8 promoter in vitro and that nuclear translocation of NF-kappaB occurs at an early time point following poly ( I:C ) stimulation of esophageal epithelial cells ... Importantly, we also show that poly ( I:C ) stimulation induces the NF-kappaB dependent esophageal epithelial expression of TLR2 , leading to enhanced epithelial responsiveness of EPC2-hTERT cells to TLR2 ligand stimulation, suggesting an important regulatory role for TLR3 mediated NF-kappaB signaling in the innate immune response of esophageal epithelial cells ... Importantly, we also show that poly ( I:C ) stimulation induces the NF-kappaB dependent esophageal epithelial expression of TLR2, leading to enhanced epithelial responsiveness of EPC2-hTERT cells to TLR2 ligand stimulation, suggesting an important regulatory role for TLR3 mediated NF-kappaB signaling in the innate immune response of esophageal epithelial cells ... Our findings demonstrate for the first time that TLR3 is highly functional in the human esophageal epithelium and that TLR3 mediated NF-kappaB signaling may play an important regulatory role in esophageal epithelial homeostasis
De Santa et al., EMBO J 2009 : Jmjd3 , a JmjC family histone demethylase, is induced by the transcription factor NF-kB in response to microbial stimuli
Mertens et al., PLoS Pathog 2009 (Abdominal Abscess) : In CD8 ( + ) CD28 ( - ) T cells, Sp1 enhances Zap-70 phosphorylation and increasingly involves NF-kappaB which ultimately results in protection versus apoptosis and cell death and promotes survival and accumulation of the CD8 ( + ) CD28 ( - ) population ... In CD8 ( + ) CD28 ( - ) T cells, Sp1 enhances Zap-70 phosphorylation and increasingly involves NF-kappaB which ultimately results in protection versus apoptosis and cell death and promotes survival and accumulation of the CD8 ( + ) CD28 ( - ) population
Wang et al., J Cell Physiol 2010 (Cardiomegaly) : Pharmacological activator of AMPK could inhibit AngII induced NF-kappaB translocation, and inhibitor of AMPK or a dominant negative AMPK adenovirus suppressed gAd mediated inhibition of I-kappaB phosphorylation and NF-kappaB activation ... Our data indicate that gAd inhibits cardiac hypertrophic signaling through AMPK mediated suppression of NF-kappaB activation
Yan et al., Zhonghua Wai Ke Za Zhi 2009 (Disease Models, Animal...) : To investigate the effects of nuclear factor kappa B (NF-kappaB) on insulin signaling in skeletal muscle cells of rat with sepsis
Totlandsdal et al., Toxicol In Vitro 2010 : The findings suggest that ultrafine carbon black induced release of IL-6 strongly depends on IL-1 and that activation of MAPKs and NF-kappaB is involved in this response
Haddad et al., Neuroscience 2009 : In this study, the role of endotoxin ( ET/LPS ) in regulating NF-kappaB was deciphered in various compartments of the CNS ... Additionally, thymulin attenuated the ET-induced response, with particular involvement of the transduction pathway implicating IkappaB-alpha , the major cytosolic inhibitor of NF-kappaB
Tzeng et al., Pharmacol Res 2010 : In this study, we examined the role of phosphatidylcholine-phospholipase C ( PC-PLC ) and protein kinase C ( PKC ) in peptidoglycan ( PGN ) -induced nuclear factor-kappaB (NF-kappaB) activation and cyclooxygenase-2 (COX-2) expression in RAW 264.7 macrophages ... Our data demonstrate that PGN activates PC-PLC which induces PKC activation ; this in turn initiates NF-kappaB activation, and ultimately induces COX-2 expression in RAW 264.7 macrophages
Zheng et al., Int J Biochem Cell Biol 2009 (Neoplasms...) : Biochemical studies revealed that CD146 was required for the activation of p38/IKK/NF kappaB signaling cascade and up-regulation of NF kappaB downstream pro-angiogenic genes, notably IL-8, ICAM-1 and MMP9, in response to tumor secretions
Tacon et al., Am J Respir Cell Mol Biol 2010 (Inflammation...) : We therefore investigated the role of NF-kappaB in HRV induced MMP-9 expression ... The role of NF-kappaB in HRV induced MMP-9 expression was further confirmed using MMP-9 promoter luciferase constructs, which demonstrated that an NF-kappaB site at -620/-607 base pairs was necessary for HRV induced MMP-9 expression
Lu et al., Arch Pharm Res 2009 : The reporter gene assay also showed that the activation of NF-kappaB induced by LPS was inhibited by pre-treatment with Timosaponin B-II
Potapovich et al., Br J Pharmacol 2009 : Phosphorylation of extracellular signal regulated kinase ( ERK1/2 ) and Akt1 but not NFkappaB was activated by AFP alone or by its combination with UVA
Im et al., Mol Immunol 2009 : Furthermore, NF-kappaB and NF-IL6 transcription factors played an important role in the flagellin induced IL-8 gene expression in A549 cells
He et al., Clinical and vaccine immunology : CVI 2009 (Mycoplasma Infections) : Furthermore, LAMP induced NF-kappaB activation was increased in 293T cells transfected with TLR2 plasmid ... The activity of NF-kappaB was synergically augmented by cotransfected TLR1, TLR6 , and CD14 ... The activity of NF-kappaB was synergically augmented by cotransfected TLR1, TLR6, and CD14 ... The activity of NF-kappaB was synergically augmented by cotransfected TLR1, TLR6 , and CD14
Asghar et al., American journal of physiology. Renal physiology 2009 (Hypertension...) : These results suggest that LPS differentially regulates NF-kappaB and Nrf2, produces inflammation, decreases antioxidant enzyme, increases oxidative stress, and causes D1 receptor dysfunction in the RPTs
Amith et al., Cell Signal 2010 : Here, we show that endotoxin lipopolysaccharide (LPS) induced MyD88/TLR4 complex formation and subsequent NFkappaB activation is dependent on the removal of alpha-2,3-sialyl residue linked to beta-galactoside of TLR4 by the Neu1 activity associated with LPS stimulated live primary macrophage cells, macrophage and dendritic cell lines but not with primary Neu1-deficient macrophage cells ... Here, we show that endotoxin lipopolysaccharide (LPS) induced MyD88/TLR4 complex formation and subsequent NFkappaB activation is dependent on the removal of alpha-2,3-sialyl residue linked to beta-galactoside of TLR4 by the Neu1 activity associated with LPS stimulated live primary macrophage cells, macrophage and dendritic cell lines but not with primary Neu1-deficient macrophage cells
van Steensel et al., Invest Ophthalmol Vis Sci 2010 (Graves Ophthalmopathy) : PDGF-BB induced NF-kappaB activity in orbital fibroblasts, and both NF-kappaB inhibitors and imatinib mesylate reduced PDGF-BB induced cytokine production
Daffis et al., PLoS Pathog 2009 (West Nile Fever) : However, pharmacological inhibition of NF-kappaB and ATF-2/c-Jun, the two other known components of the IFN-beta enhanceosome, strongly reduced IFN-beta gene transcription in the DKO dendritic cells
Ali et al., Int J Biochem Cell Biol 2010 : IL-1 induced NF-kappaB DNA binding and activation by this transcription factor and this was attenuated by curcumin and apigenin ... IL-1 induced NF-kappaB DNA binding and activation by this transcription factor and this was attenuated by curcumin and apigenin ... Taken together, these results suggest a potentially crucial role for NF-kappaB in the IL-1 induced expression of C/EBPdelta , and thereby downstream APP genes regulated by this transcription factor ... Taken together, these results suggest a potentially crucial role for NF-kappaB in the IL-1 induced expression of C/EBPdelta, and thereby downstream APP genes regulated by this transcription factor
Guo et al., Biochim Biophys Acta 2009 : Here, we report that NF-kappaB site mediated cyclin D1 transcription is inhibited by heat shock
Braghin et al., Neurosci Lett 2009 : Alpha1-antichymotrypsin induces TNF-alpha production and NF-kappaB activation in the murine N9 microglial cell line
Oakley et al., J Biol Chem 2009 : Lipid rafts and caveolin-1 coordinate interleukin-1beta (IL-1beta) dependent activation of NFkappaB by controlling endocytosis of Nox2 and IL-1beta receptor 1 from the plasma membrane ... Lipid rafts and caveolin-1 coordinate interleukin-1beta (IL-1beta) dependent activation of NFkappaB by controlling endocytosis of Nox2 and IL-1beta receptor 1 from the plasma membrane ... Finally, we demonstrate that Vav1 , a Rac1 guanine exchange factor and activator of Nox2, is recruited to lipid rafts following IL-1beta stimulation and that it is required for NFkappaB activation
Gao et al., Biol Pharm Bull 2009 (Brain Ischemia...) : We also determined expression of TLR4 mRNA and MyD88 mRNA by in situ hybridization ( ISH ), activation of nuclear factor (NF)-kappaB by electrophoretic mobility-shift analysis ( EMSA ), and expression of interrleukin (IL)-1beta protein by Western blot ... We also determined expression of TLR4 mRNA and MyD88 mRNA by in situ hybridization ( ISH ), activation of nuclear factor (NF)-kappaB by electrophoretic mobility-shift analysis ( EMSA ), and expression of interrleukin (IL)-1beta protein by Western blot
Fu et al., J Vet Med Sci 2009 (Inflammation) : Vaspin did not inhibit the TNF-alpha ( 20 min ) activation of JNK, p38 and NF-kappaB , but only slightly inhibited Akt ... Vaspin did not inhibit the TNF-alpha ( 20 min ) activation of JNK, p38 and NF-kappaB , but only slightly inhibited Akt ... Vaspin did not inhibit the TNF-alpha ( 20 min ) activation of JNK, p38 and NF-kappaB , but only slightly inhibited Akt
Shimpo et al., J Orthop Sci 2009 (Osteoarthritis) : IL-1beta strongly increased the translocation of NF-kappaB to the nucleus and the phosphorylation of extracellular-signal regulated kinase, p38, and c-Jun amino-terminal kinase ; but the up-regulation was not inhibited by DEX or COX inhibitors ... IL-1beta strongly increased the translocation of NF-kappaB to the nucleus and the phosphorylation of extracellular-signal regulated kinase, p38, and c-Jun amino-terminal kinase ; but the up-regulation was not inhibited by DEX or COX inhibitors
Hole et al., J Agric Food Chem 2009 : In this study we aimed to examine the effect of different phenolic extracts from barley, oat, wheat, and buckwheat on the modulation of basal and lipopolysaccharide (LPS) induced NF-kappaB activity and elucidate the role of phenolic acids in this modulation ... Generally, extracts M2 showed the highest effect on modulation of NF-kappaB activity with strong inhibition of LPS induced NF-kappaB activity at all concentrations and of the basal NF-kappaB activity at concentrations equal to or lower than 3 mg/mL ... Most of extracts M1 and HY slightly increased both the basal and the LPS induced NF-kappaB activation ... However, at the highest concentrations ( 3 or 15 mg/mL ) extracts HY inhibited LPS induced NF-kappaB activation
dos Santos et al., J Ethnopharmacol 2010 (Disease Models, Animal...) : In addition, the in vitro effects of BdE on COX-2 activity and on the activation of NF-kappaB were also evaluated
Lee et al., Cancer Res 2009 : Furthermore, inhibition of Tpl2 reduced the arsenite induced promoter activity of NF-kappaB and activator protein-1 (AP-1), indicating that NF-kappaB and AP-1 are downstream transducers of arsenite triggered Tpl2
Yin et al., Biochemistry 2009 : It has been assumed that TRAF2 is a ubiquitin ligase like TRAF6 and mediates K63 linked polyubiquitination of RIP1, a kinase pivotal in TNFalpha induced NF-kappaB activation
Cha et al., Immunol Invest 2009 (Inflammation) : Western blot analysis showed that the essential oil inhibited the phosphorylation of IkappaB-alpha, nuclear translocation of p65 , and subsequent activation of NF-kappaB
Graham et al., Cancer Sci 2010 (Neoplasm Metastasis...) : We recently reported that phosphoinositide 3-kinase (PI3K)/protein kinase B ( Akt ) mediates transcriptional regulation and activation of bone morphogenetic protein (BMP)-2 signaling by nuclear factor (NF)-kappaB in bone metastatic prostate cancer cells ... We recently reported that phosphoinositide 3-kinase (PI3K)/protein kinase B ( Akt ) mediates transcriptional regulation and activation of bone morphogenetic protein (BMP)-2 signaling by nuclear factor (NF)-kappaB in bone metastatic prostate cancer cells ... We recently reported that phosphoinositide 3-kinase (PI3K)/protein kinase B ( Akt ) mediates transcriptional regulation and activation of bone morphogenetic protein (BMP)-2 signaling by nuclear factor (NF)-kappaB in bone metastatic prostate cancer cells
Pei et al., Int Immunopharmacol 2010 (Prostatic Neoplasms) : Lipopolysaccharide (LPS) induced TLR4-NF-(K)B signaling plays an important role in the development of prostatic tumors from chronic bacterial prostatic infection
Brahmachari et al., J Immunol 2009 : Although NaB reduced the level of cholesterol in vivo in mice, reversal of the inhibitory effect of NaB on iNOS expression, and NF-kappaB activation by hydroxymethylglutaryl-CoA , mevalonate, and farnesyl pyrophosphate, but not cholesterol and ubiquinone, suggests that depletion of intermediates, but not end products, of the mevalonate pathway is involved in the anti-inflammatory effect of NaB ... Although NaB reduced the level of cholesterol in vivo in mice, reversal of the inhibitory effect of NaB on iNOS expression, and NF-kappaB activation by hydroxymethylglutaryl-CoA, mevalonate, and farnesyl pyrophosphate, but not cholesterol and ubiquinone, suggests that depletion of intermediates, but not end products, of the mevalonate pathway is involved in the anti-inflammatory effect of NaB
Harder et al., J Immunol 2009 (Disease Models, Animal...) : These results indicate that caspase-1 activation in response to S. pyogenes infection requires NF-kappaB and the virulence factor streptolysin O, but proceeds independently of P2X7R and TLR signaling
Schumacher et al., Biochem Pharmacol 2010 : Heteronemin, a spongean sesterterpene, inhibits TNF alpha induced NF-kappa B activation through proteasome inhibition and induces apoptotic cell death
Yang et al., J Neuroimmunol 2009 : Consistent with this finding, BE inhibited LPS induced IkappaBalpha degradation, NF-kappaB nuclear translocation, and also Akt, c-Jun NH2-terminal kinase (JNK) phosphorylation
Baker et al., Journal of molecular cell biology 2010 : The inhibitor of kB kinase (IKK) complex , a critical positive regulator of nuclear factor kappaB (NF-kappaB) signaling and immune function, can be activated in vitro by polyubiquitin chains
Wang et al., Cytokine 2010 : ApoA-I diminished LPS induced mCD14 expression ( P < 0.01 ) and NFkappaB nuclear translocation in THP-1 cells ... ApoA-I diminished LPS induced mCD14 expression ( P < 0.01 ) and NFkappaB nuclear translocation in THP-1 cells
Lamb et al., EMBO Rep 2009 (Inflammation) : Helicobacter pylori CagA activates NF-kappaB by targeting TAK1 for TRAF6 mediated Lys 63 ubiquitination ... CagA physically associates with TAK1 and enhances its activity and TAK1 induced NF-kappaB activation through the tumour necrosis factor receptor associated factor 6-mediated, Lys 63-linked ubiquitination of TAK1 ... CagA physically associates with TAK1 and enhances its activity and TAK1 induced NF-kappaB activation through the tumour necrosis factor receptor associated factor 6-mediated, Lys 63-linked ubiquitination of TAK1
Lin et al., Phytother Res 2010 : Moreover, baicalin inhibited the nuclear translocation of NF-kappaB induced by H/R or TNF-alpha
Deo et al., J Biochem Mol Toxicol 2009 : Stimulation of human microvascular endothelial cells with BSA-LMM-AGE-Cas complexes significantly increased mRNA expression of the receptor of AGE (RAGE), galectin-3 ( AGE-R3 ), tumor necrosis factor alpha, and a marker of the mitogen activated protein kinase pathway ( MAPK-1 ), as well as p65NF-kappaB activation ... Stimulation of human microvascular endothelial cells with BSA-LMM-AGE-Cas complexes significantly increased mRNA expression of the receptor of AGE (RAGE), galectin-3 ( AGE-R3 ), tumor necrosis factor alpha, and a marker of the mitogen activated protein kinase pathway ( MAPK-1 ), as well as p65NF-kappaB activation
Yeligar et al., J Immunol 2009 : Ethanol mediated activation of NF-kappaB led to HIF-1alpha activation and concomitant RANTES expression
Darwech et al., J Cell Biochem 2009 (Osteolysis) : Further, CC2 and LZ peptides attenuate RANKL- and TNF induced NF-kappaB signaling in bone marrow derived osteoclast precursors ( OCPs )
Chang et al., J Pharmacol Sci 2009 : In conclusion, the present study is the first to demonstrate that FC-77 decreases LPS induced NO production in macrophages, which may be associated with the suppression of pro-inflammatory cytokines, and ROS production, as well as NF-kappaB activation
Lee et al., J Pharmacol Sci 2009 (Neurotoxicity Syndromes) : Moreover, ISL impeded the expression of nitric oxide synthase and the activation of NF-kappaB through blockage of its phosphorylation
Henrotin et al., Osteoarthritis Cartilage 2010 (Inflammation...) : Curcumin blocks IL-1beta induced proteoglycan degradation, AP-1/NF-kappaB signalling, chondrocyte apoptosis and activation of caspase-3
Ma et al., J Biol Chem 2009 (Neoplasm Invasiveness...) : Furthermore, silencing EGR-1 suppressed a synergistically functional interaction between EGR-1 and NF-kappaB
Shih et al., Eur J Immunol 2009 (Inflammatory Bowel Diseases) : TL1A induced by bacteria is mediated in part by the TLR signaling pathway and inhibited by downstream blockade of p38 MAPK and NF-kappaB activation
Chun et al., Int Immunopharmacol 2010 (Necrosis) : CD14 played a significant role in the activation of NF-kappaB in response to necrotic cells in the presence or absence of TLR2
Greene et al., Eur Respir J 2010 (Emphysema...) : In 16HBE14o- cells but not HEK293 cells, ZAAT upregulated expression of cIAP-1 , an upstream regulator of NF-kappaB
Fujisawa et al., J Immunol 2009 : NF-kappaB activation in airway epithelial cells has been shown to play a positive inflammatory role in chronic airway diseases ; however, the role of NF-kappaB in mucin gene expression is unresolved
Katada et al., Inflammation 2010 (Inflammatory Bowel Diseases...) : The obtained results indicate that tissue levels of TNF-alpha, E-selectin and ICAM-1 protein expression, activation of NF-kappaB , and subsequent accumulation of PMN were elevated in I/R challenged jejunum ... The obtained results indicate that tissue levels of TNF-alpha , E-selectin and ICAM-1 protein expression, activation of NF-kappaB , and subsequent accumulation of PMN were elevated in I/R challenged jejunum ... The obtained results indicate that tissue levels of TNF-alpha, E-selectin and ICAM-1 protein expression, activation of NF-kappaB , and subsequent accumulation of PMN were elevated in I/R challenged jejunum
Chang et al., Inflammation 2010 (Inflammation) : Moreover, LPS induced nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) nuclear protein-DNA binding affinity and reporter gene activity were significantly decreased by LA and GLA
Yu et al., FEBS J 2009 : NF-kappaB regulates the transcription of protein tyrosine kinase Tec ... This study, together with our previous findings on the transcriptional regulation of Btk ( Bruton 's tyrosine kinase ) by proteasome inhibitors, provides important insight into the molecular mechanism ( s ) underlying the role of NF-kappaB in Tec family kinase signaling and lymphocyte development
Liu et al., J Biol Chem 2009 : NF-kappaB p65, induced by IL-1beta , interacts with a novel element in the PDGF-D promoter and inhibits PDGF-D transcription
Gan et al., J Cell Sci 2009 : HSCARG inhibits activation of NF-kappaB by interacting with IkappaB kinase-beta ... Here, we show that HSCARG is involved in the NF-kappaB signaling pathway and find that HSCARG suppresses TNF- and IL1 induced NF-kappaB activation in a dose dependent manner ... In addition, our data indicate that IKKbeta is required for HSCARG inhibited NF-kappaB activation
Baritaki et al., Cancer Res 2009 (Adenocarcinoma...) : We show that NPI-0052 inhibits both NF-kappaB and Snail and induces RKIP expression, thus resulting in cell sensitization to CDDP and TRAIL
Hoek et al., J Immunol 2009 (Lymphopenia) : Recent studies have shown that BCR signaling positively regulates NF-kappaB2 , suggesting BCR regulation of BR3 signaling
Yamazaki et al., Science signaling 2009 : Although transforming growth factor-beta activated kinase 1 ( TAK1 ) and mitogen activated protein kinase ( MAPK ) kinase kinase 3 ( MEKK3 ) are both crucial for IL-1 dependent activation of NF-kappaB , their potential functional and physical interactions remain unclear ... Here, we showed that TAK1 mediated activation of NF-kappaB required the transient formation of a signaling complex that included tumor necrosis factor receptor associated factor 6 ( TRAF6 ), MEKK3, and TAK1 ... After TAK1 mediated activation of NF-kappaB, TRAF6 subsequently activated NF-kappaB through MEKK3 independently of TAK1, thereby establishing continuous activation of NF-kappaB, which was required for the production of sufficient cytokines ... After TAK1 mediated activation of NF-kappaB , TRAF6 subsequently activated NF-kappaB through MEKK3 independently of TAK1, thereby establishing continuous activation of NF-kappaB, which was required for the production of sufficient cytokines
Milkova et al., Exp Dermatol 2010 : Because LPS initiates signalling via the receptor Toll-like receptor 4 (TLR4) and the consequent activation of the transcription factor nuclear factor-kappaB (NF-kappaB) , we investigated TLR4 expression and NF-kappaB regulation in human Treg
McAllister et al., J Virol 2010 : Furthermore, protein kinase PKR and mitochondrial adapter IPS-1 were required for maximal C ( ko ) -mediated IFN-beta induction, which correlated with the PKR mediated enhancement of mitogen activated protein kinase and NF-kappaB activation
Okumura et al., J Virol 2010 (Inflammation) : Ebola virus GP was found to interact with TLR4 by immunoprecipitation/Western blot analyses, and Ebola virus GP on VLPs was able to stimulate expression of NF-kappaB in a TLR4 dependent manner
Kure et al., J Pharmacol Exp Ther 2010 (Inflammation) : LXA4 also reduced the LPS induced nuclear translocation of nuclear factor-kappaB (NF-kappaB)
Lee et al., J Immunol 2009 (Inflammation...) : APE1 was essential for the transcriptional activation and nuclear translocation of hypoxia-inducible factor-1alpha and NF-kappaB , both of which are crucial for inflammatory signaling in keratinocytes
Jeong et al., J Immunol 2009 (Arthritis, Rheumatoid) : Site directed mutagenesis revealed that Pin1 mediated transcriptional activation of COX-2 was coordinately regulated by NF-kappaB , CREB, and C/EBP
Meylan et al., Nature 2009 (Adenocarcinoma...) : Concomitant loss of p53 ( also known as Trp53 ) and expression of oncogenic Kras ( G12D ) resulted in NF-kappaB activation in primary mouse embryonic fibroblasts ... Concomitant loss of p53 ( also known as Trp53 ) and expression of oncogenic Kras ( G12D ) resulted in NF-kappaB activation in primary mouse embryonic fibroblasts
Barbie et al., Nature 2009 (Lung Neoplasms...) : In these cells, TBK1 activated NF-kappaB anti-apoptotic signals involving c-Rel and BCL-XL ( also known as BCL2L1 ) that were essential for survival, providing mechanistic insights into this synthetic lethal interaction
Yu et al., J Biol Chem 2009 (Inflammation...) : In addition, IDR-1 induced p38 MAPK activity in a p62 dependent manner and increased CCAAT-enhancer binding protein beta activity, whereas NF-kappaB activity was unaffected ... In addition, IDR-1 induced p38 MAPK activity in a p62 dependent manner and increased CCAAT-enhancer binding protein beta activity, whereas NF-kappaB activity was unaffected ... In addition, IDR-1 induced p38 MAPK activity in a p62 dependent manner and increased CCAAT-enhancer binding protein beta activity, whereas NF-kappaB activity was unaffected
Łabuzek et al., Neurotoxicology 2010 : Furthermore, we found that the effects of AICAR on IL-6 and TNF-alpha ( 12, 24h ) release and on the expression of iNOS and NF-kappaB p65 are not AMPK dependent because the pre-treatment of LPS activated microglia with compound C ( a pharmacological inhibitor of AMPK ) did not reverse the effect of AICAR
Schmidt et al., Biochem Pharmacol 2010 : Inhibition of p38MAPK, NF-kappaB and the JAK2-STAT-1alpha pathways resulted in a reduction of iNOS expression
Chang et al., Experimental biology and medicine (Maywood, N.J.) 2009 : The proteolytic activity of caspase 8 was required for the NF-kappaB activity ... Further studies showed that G308S could increase the phosphorylation of ERK and the translocation of NF-kappaB into the nucleus, and the inhibition of ERK phosphorylation decreased the IL-8 production, mRNA expression and activation of NF-kappaB
Yamamoto-Furusho et al., Inflamm Bowel Dis 2010 (Salmonella Infections) : HNP-1 is required for NOD2 dependent NF-kappaB activation after MDP-LD stimulation since hnp-1 siRNA transfection abrogated the response to MDP-LD stimulation ... HNP-1 is required for NOD2 dependent NF-kappaB activation after MDP-LD stimulation since hnp-1 siRNA transfection abrogated the response to MDP-LD stimulation
Shin et al., J Med Food 2009 (Carcinoma, Hepatocellular...) : They also inhibited expression of matrix metalloproteinase (MMP)-2 and MMP-9 and activation of nuclear factor kappaB (NF-kappaB) stimulated by tumor necrosis factor alpha ... They also inhibited expression of matrix metalloproteinase (MMP)-2 and MMP-9 and activation of nuclear factor kappaB (NF-kappaB) stimulated by tumor necrosis factor alpha
Shen et al., J Biol Chem 2009 (Inflammation...) : We showed that kallistatin inhibits tumor necrosis factor-alpha induced NF-kappaB activation, as well as vascular cell adhesion molecule-1 and monocyte chemoattractant protein-1 expression in endothelial cells, whereas knockdown of KLF4 by small interfering RNA oligonucleotide abolished the effect of kallistatin
Zabel et al., J Biol Chem 1991 : Purified NF-kappa B required 100 mM KCl or NaCl and a pH of 7.5 to optimally bind to DNA
Wang et al., Alcohol Clin Exp Res 2010 : The goal of this study was to test the possibility that nuclear factor-kappa B (NF-kappaB) is involved in the upregulation of inducible nitric oxide synthase (iNOS) expression induced by ethanol in colon
Liu et al., Molecular cancer 2009 (Nasopharyngeal Neoplasms) : These results suggest that human iE kappa is active in Ig kappa expressing NPC cells and LMP1 stimulated NF-kappaB and AP-1 activation results in an augmenting activation of the iE kappa
Salminen et al., Cell Signal 2010 : We will discuss the evidence that insulin/IGF-1 signaling can enhance the NF-kappaB signaling and subsequently potentiate the aging process and aggravate age related degenerative diseases ... We will discuss the evidence that insulin/IGF-1 signaling can enhance the NF-kappaB signaling and subsequently potentiate the aging process and aggravate age related degenerative diseases
Hohmann et al., Mol Cell Biol 1991 : The activation of NF-kappa B-like activities ( called NF-kappa B ) by tumor necrosis factor alpha (TNF alpha) and the phorbol ester phorbol 12-myristate 13-acetate ( PMA ) were compared ... NF-kappa B activation by TNF alpha was initially cycloheximide insensitive, but maintenance of NF-kappa B activity required ongoing protein synthesis and continuous stimulation by TNF alpha ... We suggest that cell-specific differences in the protein kinase C-dependent activation of NF-kappa B may exist and that TNF alpha and PMA may induce expression of the gene ( s ) encoding NF-kappa B
Ghosh et al., J Neurosci 2009 (Disease Models, Animal...) : First, MPP ( + ) induced the activation of p21(ras) and nuclear factor-kappaB (NF-kappaB) in mouse microglial cells ... Inhibition of MPP ( + ) -induced activation of NF-kappaB by Deltap21 ( ras ), a dominant negative mutant of p21(ras), supported the involvement of p21(ras) in MPP ( + ) -induced microglial activation of NF-kappaB
Shan et al., J Biomed Biotechnol 2009 (Brain Injuries...) : Interestingly, we found, PSPC decreased the expression level of glial fibrillary acidic protein (GFAP), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), inhibited nuclear translocation of nuclear factor-kappaB (NF-kappaB) , increased the activity of copper/zinc superoxide dismutase ( Cu/Zn-SOD ) and catalase (CAT), and reduced the content of malondialdehyde ( MDA ), respectively ... Interestingly, we found, PSPC decreased the expression level of glial fibrillary acidic protein (GFAP), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), inhibited nuclear translocation of nuclear factor-kappaB (NF-kappaB) , increased the activity of copper/zinc superoxide dismutase ( Cu/Zn-SOD ) and catalase (CAT) , and reduced the content of malondialdehyde ( MDA ), respectively ... Interestingly, we found, PSPC decreased the expression level of glial fibrillary acidic protein (GFAP), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) , inhibited nuclear translocation of nuclear factor-kappaB (NF-kappaB) , increased the activity of copper/zinc superoxide dismutase ( Cu/Zn-SOD ) and catalase (CAT), and reduced the content of malondialdehyde ( MDA ), respectively ... Interestingly, we found, PSPC decreased the expression level of glial fibrillary acidic protein (GFAP) , inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2), inhibited nuclear translocation of nuclear factor-kappaB (NF-kappaB) , increased the activity of copper/zinc superoxide dismutase ( Cu/Zn-SOD ) and catalase (CAT), and reduced the content of malondialdehyde ( MDA ), respectively ... Interestingly, we found, PSPC decreased the expression level of glial fibrillary acidic protein (GFAP), inducible nitric oxide synthase (iNOS) , and cyclooxygenase-2 (COX-2), inhibited nuclear translocation of nuclear factor-kappaB (NF-kappaB) , increased the activity of copper/zinc superoxide dismutase ( Cu/Zn-SOD ) and catalase (CAT), and reduced the content of malondialdehyde ( MDA ), respectively
Maruyama et al., J Bone Miner Res 2010 : RANKL still induced the degradation of I kappaB alpha and activated classical NF-kappaB , whereas processing of p100 to p52 was abolished by the aly/aly mutation
Zwart et al., J Bone Miner Res 2010 (Bone Resorption) : We investigated the effects of EPA on differentiation of RAW264.7 monocyte/macrophage cells induced by receptor activator of NF-kappaB ligand ( RANKL ) and on activation of NF-kappaB by tumor necrosis factor alpha (TNF-alpha) or exposure to modeled weightlessness
Sarir et al., Biochem Pharmacol 2010 (Emphysema) : Preincubation of the cells with antioxidants, such as N-acetyl-L-cysteine (NAC), prevented the overproduction of IL-8 and activation of NF-kappaB
Sun et al., Cell Signal 2010 : USP11 negatively regulates TNFalpha induced NF-kappaB activation by targeting on IkappaBalpha ... USP11 negatively regulates TNFalpha induced NF-kappaB activation by targeting on IkappaBalpha ... Ubiquitination mediated IkappaBalpha degradation immediately precedes and is required for NF-kappaB nuclear translocation and activation ... Moreover, knockdown of USP11 expression enhances TNFalpha induced IkappaBalpha ubiquitination and NF-kappaB activation ... These data demonstrate that USP11 plays an important role in the downregulation of TNFalpha mediated NF-kappaB activation through modulating IkappaBalpha stability ... These data demonstrate that USP11 plays an important role in the downregulation of TNFalpha mediated NF-kappaB activation through modulating IkappaBalpha stability ... In addition, overexpression of a catalytically inactive USP11 mutant partially inhibits TNFalpha- and IKKbeta induced NF-kappaB activation, suggesting that USP11 also exerts a non-catalytic function in its negative regulation of TNFalpha mediated NF-kappaB activation ... In addition, overexpression of a catalytically inactive USP11 mutant partially inhibits TNFalpha- and IKKbeta induced NF-kappaB activation, suggesting that USP11 also exerts a non-catalytic function in its negative regulation of TNFalpha mediated NF-kappaB activation ... In addition, overexpression of a catalytically inactive USP11 mutant partially inhibits TNFalpha- and IKKbeta induced NF-kappaB activation, suggesting that USP11 also exerts a non-catalytic function in its negative regulation of TNFalpha mediated NF-kappaB activation ... Thus, IkappaBalpha ubiquitination and deubiquitination processes function as a Yin-Yang regulatory mechanism on TNFalpha induced NF-kappaB activation
Hayakawa et al., J Am Soc Nephrol 2010 : Overexpression of C/EBPbeta markedly suppressed TNF-alpha induced activation of NF-kappaB , independent of its transacting potential ... Overexpression of C/EBPbeta markedly suppressed TNF-alpha induced activation of NF-kappaB , independent of its transacting potential
Fock et al., Inflammation 2010 (Anemia...) : Upon various signals, such as lipopolysaccharide (LPS), I kappaB alpha is rapidly degraded and NF-kappaB is induced to translocate into the nucleus, where it activates expression of various genes that participate in the inflammatory response, including those involved in the synthesis of TNF-alpha
Iliopoulos et al., Cell 2009 (Inflammation) : IL6 mediated activation of the STAT3 transcription factor is necessary for transformation, and IL6 activates NF-kappaB , thereby completing a positive feedback loop ... IL6 mediated activation of the STAT3 transcription factor is necessary for transformation, and IL6 activates NF-kappaB , thereby completing a positive feedback loop ... IL6 mediated activation of the STAT3 transcription factor is necessary for transformation, and IL6 activates NF-kappaB , thereby completing a positive feedback loop
Cantrell et al., Immunobiology 2010 (Melanoma, Experimental) : Activation of both cell types is associated with the induction of the MAP kinase pathway, the phosphorylation of STAT1 , STAT5 and AKT and with transcription factor NF-kappaB activation in vitro and in vivo
di Paola et al., Free radical research 2010 (Disease Models, Animal...) : CA1 also reduced NF-kappaB activation, the pro-inflammatory cytokines release, the appearance of I-NOS, nitrotyrosine, PARP and proMMP-9 and -2 activity in the colon and reduced the up-regulation of ICAM-1 and the expression of P-Selectin
Samanta et al., Cancer Sci 2010 (Burkitt Lymphoma) : In BL cells, EBERs activate RIG-I signaling and induce the expression of type-I IFNs and interferon stimulated genes ( ISGs ) through the activation of RIG-I substrates, nuclear factor-kappa B (NF-kappaB), and IFN regulatory factor 3 (IRF-3), and anti-inflamatory cytokine IL-10 through IRF-3 but not NF-kappaB signaling ... In BL cells, EBERs activate RIG-I signaling and induce the expression of type-I IFNs and interferon stimulated genes ( ISGs ) through the activation of RIG-I substrates, nuclear factor-kappa B (NF-kappaB), and IFN regulatory factor 3 (IRF-3) , and anti-inflamatory cytokine IL-10 through IRF-3 but not NF-kappaB signaling ... In BL cells, EBERs activate RIG-I signaling and induce the expression of type-I IFNs and interferon stimulated genes ( ISGs ) through the activation of RIG-I substrates, nuclear factor-kappa B (NF-kappaB) , and IFN regulatory factor 3 (IRF-3), and anti-inflamatory cytokine IL-10 through IRF-3 but not NF-kappaB signaling
Bergqvist et al., Proc Natl Acad Sci U S A 2009 : We present results from real-time binding kinetic experiments, demonstrating that IkappaBalpha increases the dissociation rate of NF-kappaB from the DNA in a highly efficient kinetic process
Dauletbaev et al., Respiration 2010 (Cystic Fibrosis) : Both TNF-alpha and IL-1beta activated NF-kappaB and stimulated IL-8 production ... Both TNF-alpha and IL-1beta activated NF-kappaB and stimulated IL-8 production
Zaidi et al., Helicobacter 2009 (Helicobacter Infections) : Immunoblot assay was performed for the analysis of AID, NF-kappaB, inhibitors of NF-kappaB ( IkappaB ), and IkappaB kinase (IKK) complex regulation with or without curcumin ... Immunoblot assay was performed for the analysis of AID, NF-kappaB, inhibitors of NF-kappaB ( IkappaB ), and IkappaB kinase (IKK) complex regulation with or without curcumin
Csaki et al., Arthritis Res Ther 2009 (Inflammation) : IL-1beta induced NF-kappaB activation was suppressed directly by cocktails of curcumin and resveratrol through inhibition of Ikappakappa and proteasome activation, inhibition of IkappaBalpha phosphorylation and degradation, and inhibition of nuclear translocation of NF-kappaB
Ohdaira et al., Comput Biol Chem 2009 (Lung Neoplasms) : Using a luciferase reporter assay, we also showed that hsa-miR-601 specifically repressed nuclear factor-kappaB (NF-kappaB) transcription factor dependent reporter expression, a key component of the immune-oncogenesis pathway ... Using a luciferase reporter assay, we also showed that hsa-miR-601 specifically repressed nuclear factor-kappaB (NF-kappaB) transcription factor dependent reporter expression, a key component of the immune-oncogenesis pathway ... Using a luciferase reporter assay, we also showed that hsa-miR-601 specifically repressed nuclear factor-kappaB (NF-kappaB) transcription factor dependent reporter expression, a key component of the immune-oncogenesis pathway
Cole et al., J Leukoc Biol 2010 (Inflammation) : We reported previously that Ft LVS elicited strong, dose dependent NF-kappaB reporter activity in TLR2 expressing human embryo kidney 293 T cells and that Ft LVS induced murine macrophage proinflammatory cytokine gene and protein expression is TLR2 dependent
Dai et al., J Immunol 2009 (Inflammation) : In this study, we report that activation of both STAT-1 and NF-kappaB signaling is essential for Con A-induced inducible NO synthase (iNOS) and NO in murine splenocytes
Qin et al., J Immunol 2009 (Arthritis, Experimental) : HMGB1 promotes the phosphorylation of MAPK p38 and the activation of NF-kappaB through RAGE, and then enhances the expression of proinflammatory cytokines ... These results demonstrate that HMGB1 enhances the proinflammatory activity of LPS by promoting the phosphorylation of MAPK p38 and by the activation of NF-kappaB through RAGE
Braun et al., J Invest Dermatol 2010 (Lymphoma, Large-Cell, Anaplastic...) : CD30/CD95 crosstalk experiments showed that CD30 ligation leads to NF-kappaB mediated c-FLIP upregulation in cALCL cells, which in turn conferred enhanced resistance to CD95 mediated apoptosis
Gao et al., Cancer 2010 (Adenocarcinoma...) : Overexpression of miR-218 also inhibited NF-kappaB transcriptional activation and transcription of cyclooxygenase -2, a proliferative gene regulated by NF-kappaB
Gramling et al., Exp Cell Res 2010 (Breast Neoplasms...) : Intracellularly, APC activates ERK, Akt, and NFkappaB , but not the JNK pathway to promote MDA-MB-231 cell motility
Dai et al., BMC cancer 2009 (Prostatic Neoplasms) : SH122 also suppressed TRAIL induced NF-kappaB activation by preventing cytosolic IkappaB-alpha degradation and RelA nuclear translocation, as well as by suppressing NF-kappaB target gene expression
Wang et al., Am J Surg 2010 (Blood Coagulation Disorders...) : Intra-abdominal sepsis led to significant decreases in colonic anastomotic bursting pressures, perianastomotic tissue HP contents, GSH levels, and plasma levels of PC, along with increases in perianastomotic tissue MPO activity, MDA levels, NF-kappaB activation , and plasma levels of TNF-alpha , IL-6, and d-dimer ... Intra-abdominal sepsis led to significant decreases in colonic anastomotic bursting pressures, perianastomotic tissue HP contents, GSH levels, and plasma levels of PC, along with increases in perianastomotic tissue MPO activity, MDA levels, NF-kappaB activation , and plasma levels of TNF-alpha, IL-6 , and d-dimer
Khan et al., Mol Cell Biochem 2010 (Escherichia coli Infections...) : In this article, we have shown that EAEC induced activation of mitogen activated protein kinases ( MAPK ) ( ERK-1/2, JNK and p38MAPK ) in cultured human intestinal epithelial cells ( INT-407 ) leads to the induction of DNA binding activity of NF-kappaB and AP-1, resulting in IL-8 production ... In this article, we have shown that EAEC induced activation of mitogen activated protein kinases ( MAPK ) ( ERK-1/2, JNK and p38MAPK ) in cultured human intestinal epithelial cells ( INT-407 ) leads to the induction of DNA binding activity of NF-kappaB and AP-1, resulting in IL-8 production ... In this article, we have shown that EAEC induced activation of mitogen activated protein kinases ( MAPK ) ( ERK-1/2 , JNK and p38MAPK ) in cultured human intestinal epithelial cells ( INT-407 ) leads to the induction of DNA binding activity of NF-kappaB and AP-1, resulting in IL-8 production
Sorriento et al., Molecular cancer 2009 (Inflammation...) : In vitro, adenovirus mediated GRK5-RH overexpression ( AdGRK5-NT ) in human tumor cells ( KAT-4 ) induces IkappaB accumulation and inhibits NFkappaB transcriptional activity leading to apoptotic events
Li et al., Immunol Lett 2010 (Arthritis, Rheumatoid...) : IL-23 induces receptor activator of NF-kappaB ligand expression in fibroblast-like synoviocytes via STAT3 and NF-kappaB signal pathways
Flister et al., Blood 2010 (Inflammation) : Inflammation induces lymphangiogenesis through up-regulation of VEGFR-3 mediated by NF-kappaB and Prox1 ... Collectively, our findings suggest that induction of the NF-kappaB pathway by inflammatory stimuli activates Prox1, and both NF-kappaB and Prox1 activate the VEGFR-3 promoter leading to increased receptor expression in lymphatic endothelial cells ... Collectively, our findings suggest that induction of the NF-kappaB pathway by inflammatory stimuli activates Prox1 , and both NF-kappaB and Prox1 activate the VEGFR-3 promoter leading to increased receptor expression in lymphatic endothelial cells
Rattner et al., Mol Cell Biol 1991 : Thus, it appears that the T-cell-specific activation of the proenkephalin promoter is mediated by NF-kappa B
Xu et al., J Biol Chem 2010 : Ubiquitination and deubiquitination of receptor interacting protein 1 (RIP1) play an important role in the positive and negative regulation of the tumor necrosis factor alpha (TNFalpha) induced nuclear factor kappaB (NF-kappaB) activation ... Notably, knockdown of USP21 in HeLa cells enhances TNFalpha induced RIP1 ubiquitination, IkappaB kinase beta (IKKbeta), and NF-kappaB phosphorylation, inhibitor of NF-kappaB alpha ( IkappaB alpha ) phosphorylation and ubiquitination, as well as NF-kappaB dependent gene expression ... Therefore, our results demonstrate that USP21 plays an important role in the down-regulation of TNFalpha induced NF-kappaB activation through deubiquitinating RIP1 ... Therefore, our results demonstrate that USP21 plays an important role in the down-regulation of TNFalpha induced NF-kappaB activation through deubiquitinating RIP1
Chen et al., Am J Respir Crit Care Med 2010 (Disease Models, Animal...) : The effect of serum amyloid A on nuclear factor (NF)-kappaB induction, cytokine expression, and Toll-like receptor-2 stimulation was determined with transformed human cell lines and bronchoalveolar lavage cells from patients with sarcoidosis
Nihira et al., Cell Death Differ 2010 : Pim-1 controls NF-kappaB signalling by stabilizing RelA/p65
Nagineni et al., Biochem Biophys Res Commun 2010 : TNF-alpha+IL-1 induced IL-11 secretion and this production was inhibited by NFkappaB pathway inhibitors
Lohoff et al., J Biol Chem 2010 : We also show that Egr-1, nuclear factor of activated T cell, and NF-kappaB cooperatively bind to an NFAT/NF-kappaB overlapping IL-4 enhancer element and activate the IL-4 promoter synergistically
Cheung et al., J Immunol 2009 : BTLA and CD160 can directly activate the HVEM dependent NF-kappaB RelA transcriptional complex raising the question of how NF-kappaB activation is repressed in naive T cells ... BTLA and CD160 can directly activate the HVEM dependent NF-kappaB RelA transcriptional complex raising the question of how NF-kappaB activation is repressed in naive T cells
Goh et al., Br J Pharmacol 2009 : Pretreatment with K-14585 inhibited PAR(2) mediated p65 NFkappaB phosphorylation and NFkappaB-DNA binding
Tusche et al., J Exp Med 2009 : We show that although MALT1 is largely dispensable for canonical NF-kappaB signaling downstream of the B cell receptor, the absence of MALT1 results in impaired BAFF induced phosphorylation of NF-kappaB2 ( p100 ), p100 degradation, and RelB nuclear translocation in B220 ( + ) B cells
Sunil et al., Int J Colorectal Dis 2010 : The influence of NFkappaB inhibitors ( dexamethasone, pyrrolidine dithiocarbamate ( PDTC ) and BAY 11-7082 ) on IL-1beta induced NFkappaB transcriptional activity was investigated by transient transfection of Caco-2 cells with an NFkappaB secreted alkaline phosphatase reporter plasmid
Hilmenyuk et al., Immunology 2010 (Food Hypersensitivity) : Finally, expression of the receptor for advanced glycation endproducts ( RAGE ) and activation of the transcription factor nuclear factor (NF)-kappaB by AGE were investigated ... Finally, expression of the receptor for advanced glycation endproducts ( RAGE ) and activation of the transcription factor nuclear factor (NF)-kappaB by AGE were investigated ... AGE-OVA exposed immature DCs showed a stronger expression of RAGE and activation of the transcription factor NF-kappaB compared with OVA loaded immature DCs
Choi et al., Food Chem Toxicol 2010 (Lung Neoplasms...) : Suppression of PMA induced tumor cell invasion and metastasis by aqueous extract isolated from Prunella vulgaris via the inhibition of NF-kappaB dependent MMP-9 expression
Takayasu et al., Neuroimmunomodulation 2010 : Cytokine induced Nurr-1 transcription and Nurr-1 transcription induced by overexpression of NF-kappaB were both blunted by mutagenesis within the NF-kappaB responsive element, which implies that Nurr-1 upregulation specifically requires NF-kappaB binding to its own DNA binding site
Brandenburg et al., Inflamm Res 2010 (Inflammation) : SF inhibits LPS stimulated ERK1/2 and JNK phosphorylation and thereby inhibits the LPS induced activation of NF-kappaB- and activator protein-1 (AP-1)
Wong et al., Cell Death Differ 2010 : RIPK1 is not essential for TNFR1 induced activation of NF-kappaB ... RIPK1 is not essential for TNFR1 induced activation of NF-kappaB ... Furthermore, several cell types from E18 RIPK1 ( -/- ) embryos seem to activate NF-kappaB in response to TNF
Shultz et al., J Interferon Cytokine Res 2009 : IFN-gamma does not affect the transient activation of classical NF-kappaB by IL-1beta and synergistic induction of ip-10 expression by IFN-gamma and IL-1beta occurs even after the activation of classical NF-kappaB has returned to basal levels ... IFN-gamma does not affect the transient activation of classical NF-kappaB by IL-1beta and synergistic induction of ip-10 expression by IFN-gamma and IL-1beta occurs even after the activation of classical NF-kappaB has returned to basal levels
Liu et al., Basic Clin Pharmacol Toxicol 2010 : Moreover, the LPS induced NF-kappaB activation was suppressed by the specific ERK1/2 inhibitor PD98059 ( 30 microM ) ( versus the LPS treated group, p < 0.01 ), but not by the specific p38 MAPK inhibitor SB203580 ( 25 microM )
Wu et al., Mol Cell Endocrinol 2010 (Atherosclerosis...) : LPS increased EL expression in a time and dose dependent manner in Raw264.7 cells and NFkappaB inhibitor, PDTC attenuated the effects of LPS on EL. EMSA revealed that LPS stimulated NFkappaB binding to EL promoter
Gorbacheva et al., Neuroscience 2010 : The blocking effect of APC on NF-kappaB p65 translocation was observed at 1 and 4 h after treatment of neurons with glutamate, when the NF-kappaBp 65 level in the nucleus was significantly above the basal level ... Then we investigated whether the binding of APC to EPCR/PAR-1 is required to control NF-kappaB activation ... Then we investigated whether the binding of APC to EPCR/PAR-1 is required to control NF-kappaB activation
Liu et al., Bone 2010 : Osteoinductive LIM mineralization protein-1 suppresses activation of NF-kappaB and selectively regulates MAPK pathways in pre-osteoclasts
Wu et al., Int J Biochem Cell Biol 2010 (Inflammation) : However, it is unknown how vitamin D receptor regulates IkappaBalpha , the endogenous inhibitor of NF-kappaB ... Our study provides new insight into vitamin D receptor regulation of an inhibitor of NF-kappaB in inflammation
Chou et al., Int J Radiat Oncol Biol Phys 2009 : Radiation transcriptionally induces IL-6 expression in endothelial cells through mitogen activated protein kinase/p38 mediated NF-kappaB/IkappaB ( inhibitor of NF-kappaB ) complex activation
Jacks et al., J Biol Chem 2010 : Here we report that the CK2 protein kinase, which contributes to NF-kappaB activation following UV radiation in a p38 dependent manner, physically interacts with MSK2 but not MSK1 and that CK2 inhibition specifically impairs UV-induced MSK2 kinase activation
Lin et al., Br J Anaesth 2010 : LPS also induced NF-kappaB activation, as inhibitor-kappaB degradation, NF-kappaB nuclear translocation, and NF-kappaB-DNA binding activity were significantly increased in LPS treated RAW264.7 cells
Boyd et al., J Immunol 2009 : Specifically, we demonstrate the Siglec-E expression inhibits TLR induced NF-kappaB and more importantly, the induction of the antiviral cytokines IFN-beta and RANTES
Wang et al., Cancer Res 2009 (Disease Progression...) : TRADD is a crucial transducer for TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation ... Knocking down TRADD expression in LNCaP cells impaired TNF-alpha induced NF-kappaB activation and androgen receptor repression, whereas overexpression of TRADD in C4-2B cells restored their sensitivity to TNF-alpha ... Knocking down TRADD expression in LNCaP cells impaired TNF-alpha induced NF-kappaB activation and androgen receptor repression, whereas overexpression of TRADD in C4-2B cells restored their sensitivity to TNF-alpha
Nishi et al., Rhinology 2009 : Glucocorticoids suppress NF-kappaB activation induced by LPS and PGN in paranasal sinus epithelial cells
Oh et al., Am J Chin Med 2009 : Furthermore, resveratrol inhibited extracellular signal regulated kinase ( ERK ) and p38 MAPK phosphorylation, IkappaBalpha degradation, NF-kappaB activation and cyclooxygenase (COX)-2 expression, which suggest that resveratrol inhibits IL-8 secretion by blocking MAPK phosphorylation and NF-kappaB activation
Jiang et al., Gynecol Obstet Invest 2010 (Pre-Eclampsia) : Protein kinase C-alpha activation induces NF-kB dependent VCAM-1 expression in cultured human umbilical vein endothelial cells treated with sera from preeclamptic patients
Bandyopadhaya et al., Can J Microbiol 2009 (Cholera) : Analysis of the mechanisms of Akt influences on cytokine response demonstrates that Akt promotes NF-kappaB activation
Labuzek et al., Naunyn Schmiedebergs Arch Pharmacol 2010 (Inflammation) : However, compound C down-regulated IL-1 beta, IL-6 and TNF alpha release, NO, ROS production, and AMPK activity, diminished NF-kappaB and HIF-1 alpha expression, as well as increased arginase I expression in lipopolysaccharide (LPS) stimulated microglia ... The observed alterations in the release or production of inflammatory mediators may be explained by the changes in NF-kappaB , HIF-1 alpha, and arginase I expression and 3- ( 4,5-dimethylthazol-2-yl ) -2,5-diphenyltetrazolinum bromide values in response to LPS , whereas the basis for the compound C effect on non stimulated microglia remains to be investigated
Williams et al., Glia 2010 : Additionally, apocynin was also able to reduce Tat and cytokine mediated activation of the corresponding signaling molecules Erk1/2, Jnk, and Akt with a decrease in activation and nuclear translocation of NF-kappaB , important regulators of CXCL10 induction
Wang et al., Neurochem Int 2010 (Disease Models, Animal...) : Mechanistically, PSPC strongly inhibited LPS induced phosphorylated extracellular signal regulated kinase ( ERK ) and phosphorylated c-Jun N-terminal kinase (JNK) expression and nuclear factor kappa B (NF-kappaB) activation
Mangolini et al., Apoptosis 2010 (Polycystic Kidney, Autosomal Recessive) : FC1-depletion also induced reduction in ERK1/2 kinase activation, upregulation of the pro-apoptotic protein p53 and activation of NF-kappaB , a transcription factor which reduces apoptosis in many organs and tissues ... Interestingly, selective inactivation of NF-kappaB using either an NF-kappaB decoy or parthenolide, a blocker of IKK dependent NF-kappaB activation, reduced, rather then increased, apoptosis and p53 levels in FC1 depleted cells
Kwon et al., Biochem Biophys Res Commun 2010 (Neoplasms) : Ablation of TOPK negatively regulated TRAIL mediated NF-kappaB activity ... Furthermore, expression of NF-kappaB dependent genes, FLICE-inhibitory protein (FLIP), inhibitor of apoptosis protein 1 ( c-IAP1 ), or X-linked inhibitor of apoptosis protein ( XIAP ) was reduced in TOPK depleted cells ... Furthermore, expression of NF-kappaB dependent genes, FLICE-inhibitory protein (FLIP), inhibitor of apoptosis protein 1 ( c-IAP1 ), or X-linked inhibitor of apoptosis protein ( XIAP ) was reduced in TOPK depleted cells ... Furthermore, expression of NF-kappaB dependent genes, FLICE-inhibitory protein (FLIP), inhibitor of apoptosis protein 1 ( c-IAP1 ), or X-linked inhibitor of apoptosis protein ( XIAP ) was reduced in TOPK depleted cells
Wang et al., J Mol Cell Cardiol 2010 (Heart Failure) : TNFalpha treatment was used to test the role of NFkappaB activation in HSP60 expression in a cardiac cell line
Ao et al., Cell Biol Int 2010 (Inflammation) : LPS induced NF-kappaB expression and translocation in the nucleus, but the inductive effects were inhibited by pioglitazone
Youn et al., J Immunol 2010 : SFN suppressed ligand induced and ligand independent TLR4 activation because it prevented IL-1R associated kinase-1 degradation, activation of NF-kappaB and IFN regulatory factor 3, and cyclooxygenase-2 expression induced by LPS or overexpression of TLR4 ... SFN suppressed ligand induced and ligand independent TLR4 activation because it prevented IL-1R associated kinase-1 degradation, activation of NF-kappaB and IFN regulatory factor 3, and cyclooxygenase-2 expression induced by LPS or overexpression of TLR4 ... SFN suppressed ligand induced and ligand independent TLR4 activation because it prevented IL-1R associated kinase-1 degradation, activation of NF-kappaB and IFN regulatory factor 3, and cyclooxygenase-2 expression induced by LPS or overexpression of TLR4 ... SFN formed adducts with cysteine residues in the extracellular domain of TLR4 as confirmed by liquid chromatography-tandem mass spectrometry analysis and the inhibitory effects of SFN on oligomerization and NF-kappaB activation were reversed by thiol donors ( DTT and N-acetyl-L-cysteine )
Langlet et al., Eur J Immunol 2010 : Similarly, dominant negative PKC-alpha repressed Pam ( 3 ) CSK ( 4 ) induced NF-kappaB- and AP-1-driven promoter activities in TLR2 expressing human embryonic kidney 293 T cells ... Similarly, dominant negative PKC-alpha repressed Pam ( 3 ) CSK ( 4 ) induced NF-kappaB- and AP-1-driven promoter activities in TLR2 expressing human embryonic kidney 293 T cells ... Dominant negative PKC-alpha inhibited NF-kappaB reporter activity mediated by overexpression of MyD88 but not TRIF ... Dominant negative PKC-alpha inhibited NF-kappaB reporter activity mediated by overexpression of MyD88 but not TRIF
Xu et al., Antioxid Redox Signal 2010 : Concurrently, PAMM expression completely abolished RANKL induced p100 NF-kappaB and c-Jun activation, as well as osteoclast formation
Lee et al., J Immunother 2010 (Inflammation...) : With the use of mice lacking TLR4 signaling, we show that TLR4 signaling influences tumor growth and that TLR4 signaling is a critical upstream activator of nuclear factor-kappa B (NF-kappaB) in TAMs
Madinier et al., PloS one 2009 (Brain Infarction...) : Indeed, PARP-1 contributes to the activation of the transcription factor NF-kB , which is essential to the upregulation of proinflammatory genes, in particular responsible for microglial activation/proliferation
Hayashi et al., Int J Mol Med 2010 (Adenocarcinoma...) : Unexpectedly, PTL alone did not inhibit NF-kappaB activation in cells stimulated with TNF-alpha, while heating alone inhibited NF-kappaB early after treatment and that effect faded over time
Zhou et al., Zhonghua Gan Zang Bing Za Zhi 2009 (Carcinoma, Hepatocellular...) : The expression and localization of NF-kappaB in HCC tissues are obviously different from those in the surrounding normal liver tissues, and the level of nucleoprotein NF-kappaB in HCC tissues is correlated with expressed TNF alpha, suggesting that TNF alpha can activate NF-kB , the activated NF-kB then translocates to the nucleus and plays important role in the carcinogenesis of HCC
Shi et al., J Biol Chem 2010 : The LPS effect on Kir6.1 and SUR2B expression was abolished in the presence of NF-kappaB inhibitors ... The LPS effect on Kir6.1 and SUR2B expression was abolished in the presence of NF-kappaB inhibitors
Gong et al., J Am Soc Nephrol 2010 : The ubiquitin-like protein FAT10 mediates NF-kappaB activation ... FAT10 is a TNF-alpha-inducible ubiquitin-like protein with a putative role in immune response, but whether FAT10 participates in TNF-alpha induced NF-kappaB activation is unknown ... Here, using renal tubular epithelial cells ( RTECs ) derived from FAT10 ( -/- ) and FAT10 ( +/+ ) mice, we observed that FAT10 deficiency abrogated TNF-alpha induced NF-kappaB activation and reduced the induction of NF-kappaB regulated genes ... Transduction of FAT10 ( -/- ) RTECs with FAT10 restored LMP2 expression, TNF-alpha induced IkBalpha degradation, p65 nuclear translocation, and NF-kappaB activation
Hara et al., Molecular vision 2009 : Hypoxia also attenuated both the LPS induced expression of IL-6 and IL-8, and the activation of NFkappaB in SV40-HCECs ... Hypoxia also attenuated both the LPS induced expression of IL-6 and IL-8 , and the activation of NFkappaB in SV40-HCECs ... Hypoxia also attenuated both the LPS induced expression of IL-6 and IL-8, and the activation of NFkappaB in SV40-HCECs
Anchoori et al., Bioorg Med Chem 2010 : Screening of six compounds for the ability to inhibit TNF induced NF-kappaB activity revealed that compound SK2009 was the most potent of these compounds in suppressing NF-kappaB activation in KBM-5 leukemic cells
Kim et al., Neurochem Int 2010 (Parkinson Disease) : These results collectively suggest that ROS, PI3 kinase, NF-kappaB , and AP-1 are commonly involved in 6-OHDA- and MPP ( + ) -induced MMP-9 gene expression, and that p38 MAPK is differentially involved ... These results collectively suggest that ROS, PI3 kinase, NF-kappaB , and AP-1 are commonly involved in 6-OHDA- and MPP ( + ) -induced MMP-9 gene expression, and that p38 MAPK is differentially involved
Wang et al., Bone 2010 (Bone Resorption) : Calcitonin-gene related peptide stimulates stromal cell osteogenic differentiation and inhibits RANKL induced NF-kappaB activation, osteoclastogenesis and bone resorption ... These results suggest that CGRP signaling maintains bone mass both by directly stimulating stromal cell osteoblastic differentiation and by inhibiting RANKL induced NF-kappaB activation, osteoclastogenesis, and bone resorption
Raymond et al., Biochem Pharmacol 2010 : Both LT and DN-p38 decreased IL-1beta induced NF-kappaB luciferase activity ... However, neither LT nor specific p-38 inhibitor interfered with LPS induced IkappaBalpha degradation or NF-kappaB nuclear translocation in AMs
Kang et al., The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2008 : These results suggest that HSP22 induces IL-8 in VSMCs via ERK1/2, and that transcription factor NF-kB may be required for the HSP22 induced IL-8 up-regulation
Lee et al., The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2008 : In addition, non-anticoagulant 2,3-O desulfated heparin ( ODS ) prevented NF-kappaB activation by TNFalpha , suggesting that the anti-inflammatory mechanism of heparin action in CECs lies in heparin 's ability to inhibit the expression of cell adhesion molecules, as opposed to its anticoagulant actions
Sheller et al., Exp Lung Res 2009 (Asthma...) : Accompanying the exaggerated inflammation was an increase in the cytokines granulocyte colony stimulating factor ( G-CSF ), interleukin (IL)-15, and KC. Interestingly, the counter regulatory interleukin , IL-10, was suppressed by NF-kappa B activation
Zhou et al., PLoS Pathog 2009 (Cryptosporidiosis) : NF-kappaB p65 dependent transactivation of miRNA genes following Cryptosporidium parvum infection stimulates epithelial cell immune responses
Chiba et al., Nihon rinsho. Japanese journal of clinical medicine 2009 (Helicobacter Infections...) : Here, we found that H. pylori infection triggers aberrant expression of activation induced cytidine deaminase ( AID ), a molecule that can induce DNA somatic hypermutation of immunoglobulin gene in B cells under physiological condition, in the gastric mucosa via NFkappaB dependent mechanism
O'Shea et al., Biochem J 2010 : Thr435 phosphorylation regulates RelA ( p65 ) NF-kappaB subunit transactivation
Bakken et al., Virology 2010 : We find that vGPCR activity results in phosphorylation of regulatory tyrosines in Shp2 and that in turn, Shp2 is required for vGPCR mediated activation of MEK, NFkappaB , and AP-1
Haas et al., Mol Cell 2009 : LUBAC enhances NEMO interaction with the TNF-RSC, stabilizes this protein complex, and is required for efficient TNF induced activation of NF-kappaB and JNK, resulting in apoptosis inhibition ... LUBAC enhances NEMO interaction with the TNF-RSC, stabilizes this protein complex, and is required for efficient TNF induced activation of NF-kappaB and JNK, resulting in apoptosis inhibition
Li et al., Int Immunopharmacol 2010 (Inflammation...) : AS reduced the Toll like receptor 2 (TLR2) and nucleotide binding oligomerization domain containing 2 ( Nod2 ) mRNA expressions up-regulated by heat killed S. aureus and inhibited NF-kappaB activation induced by heat killed S. aureus ... In conclusion, our results demonstrated that AS-mediated protection on septic mice challenged with S. aureus was associated with its reduction on TNF-alpha release via inhibition of TLR2 and Nod2 mRNA expressions and transcription factor NF-kappaB activation
Brunelli et al., Biochem Pharmacol 2010 (Multiple Myeloma) : We have evaluated the potency of both compounds in inducing cell death, cell cycle perturbations, apoptosis, NF-kB inhibition and GST-pi activity in human carcinoma cells with different GST-pi contents as well as in human multiple myeloma and leukaemia cell lines
Kim et al., American journal of physiology. Renal physiology 2010 (Disease Models, Animal...) : In confirmation of earlier studies, rats with chronic renal failure exhibited increased lipid peroxidation, glutathione depletion, NF-kappaB activation , mononuclear cell infiltration, and upregulation of monocyte chemoattractant protein-1, NAD ( P ) H oxidase, cyclooxygenase-2 , and 12-lipoxygenase in the remnant kidney pointing to oxidative stress and inflammation ... In confirmation of earlier studies, rats with chronic renal failure exhibited increased lipid peroxidation, glutathione depletion, NF-kappaB activation , mononuclear cell infiltration, and upregulation of monocyte chemoattractant protein-1 , NAD ( P ) H oxidase, cyclooxygenase-2, and 12-lipoxygenase in the remnant kidney pointing to oxidative stress and inflammation ... In confirmation of earlier studies, rats with chronic renal failure exhibited increased lipid peroxidation, glutathione depletion, NF-kappaB activation , mononuclear cell infiltration, and upregulation of monocyte chemoattractant protein-1, NAD ( P ) H oxidase , cyclooxygenase-2, and 12-lipoxygenase in the remnant kidney pointing to oxidative stress and inflammation
Koh et al., J Pharmacol Exp Ther 2010 (Acute Disease...) : Furthermore, we found that specific ERK1/2 and JNK inhibitors reduce NF-kappaB and AP-1 activity
Liao et al., Am J Physiol Regul Integr Comp Physiol 2010 (Endotoxemia...) : These changes were accompanied by a progressive decrease of mitochondrial aconitase activity and NF-kappaB activation
Xue et al., Cardiovasc Res 2010 (Hyperglycemia) : Also, NAC and NF-kappaB inhibitors can effectively block the high glucose induced activity of NF-kappaB binding to DNA and/or peroxide production
Lowe et al., J Biol Chem 2010 (Cell Transformation, Neoplastic...) : PPM1D promoter activity was positively regulated by NF-kappaB , and this regulation was dependent on the presence of the conserved kappaB site in the PPM1D promoter region
Hansdottir et al., J Immunol 2010 (Inflammation) : We found that vitamin D induces IkappaBalpha , an NF-kappaB inhibitor , in airway epithelium and decreases RSV induction of NF-kappaB-driven genes such as IFN-beta and CXCL10
Ashida et al., Nat Cell Biol 2010 (Inflammation...) : NF-kappaB ( nuclear factor kappaB ) has a pivotal role in many cellular processes, including the inflammatory and immune responses and, therefore, its activation is tightly regulated by the IKK ( IkappaB kinase) complex and by IkappaBalpha degradation ... NF-kappaB ( nuclear factor kappaB ) has a pivotal role in many cellular processes, including the inflammatory and immune responses and, therefore, its activation is tightly regulated by the IKK ( IkappaB kinase) complex and by IkappaBalpha degradation ... As NEMO is essential for NF-kappaB activation, we propose that the polyubiquitylation and degradation of NEMO during Shigella infection is a new bacterial strategy to modulate host inflammatory responses
Bui-Nguyen et al., Oncogene 2010 (Carcinoma, Hepatocellular) : NF-kappaB signaling mediates the induction of MTA1 by hepatitis B virus transactivator protein HBx ... Collectively, these findings revealed a previously unrecognized integral role of MTA1 in HBx stimulation of NF-kappaB signaling and consequently, the expression of NF-kappaB targets gene products with functions in inflammation and tumorigenesis
Zhang et al., Molecular vision 2009 : Furthermore, BOL-303242-X inhibited IL-1ss induced decreases in IkappaB-alpha levels, as well as IL-1ss induced phosphorylation of NFkappaB , p38, and c-Jun-N-terminal kinase (JNK) MAPKs in HCEpiCs
Pfosser et al., Stem Cells 2010 : We conclude that NF kappaB activation by LL37 or transient p65-transfection increases functionally relevant eEPC recruitment to ischemic muscle tissue via induction of PSGL-1 and E-selectin
Cheng et al., J Biomed Mater Res A 2010 (Inflammation...) : The critical role for TAK1 in p38MAPK and NFkappaB activation was as well confirmed by the inhibition of p38MAPK and NFkappaB activity following 5Z-7-oxozeaenol, a selective inhibitor of TAK1
Medici et al., Matrix Biol 2010 : Type I collagen promotes epithelial-mesenchymal transition through ILK dependent activation of NF-kappaB and LEF-1 ... Here we show that collagen I causes ILK dependent phosphorylation of IkappaB and subsequent nuclear translocation of active NF-kappaB , which in turn promotes increased expression of the Snail and LEF-1 transcription factors
Grivennikov et al., Cytokine Growth Factor Rev 2010 (Colitis...) : Other interactions and forms of crosstalk between NF-kappaB and STAT3 include physical interaction between the two, cooperation of these factors at gene promoters/enhancers, the NF-kappaB dependent expression of inhibitors of STAT3 activation and the participation of STAT3 in inflammatory cells in the negative regulation NF-kappaB
Takada et al., J Immunol 2010 (Colitis...) : These findings reveal that the absence of c-Fos and overexpression of Fra-1 respectively enhance and suppress the activation of NF-kappaB in DSS induced inflammatory stress ... These findings reveal that the absence of c-Fos and overexpression of Fra-1 respectively enhance and suppress the activation of NF-kappaB in DSS induced inflammatory stress
Mascanfroni et al., J Biol Chem 2010 : Nuclear factor (NF)-kappaB dependent thyroid hormone receptor beta1 expression controls dendritic cell function via Akt signaling
Thoh et al., J Biol Chem 2010 : Azadirachtin blocks TNF induced activation of nuclear factor kappaB (NF-kappaB) and also expression of NF-kappaB dependent genes such as adhesion molecules and cyclooxygenase 2 ... Azadirachtin blocks TNF induced activation of nuclear factor kappaB (NF-kappaB) and also expression of NF-kappaB dependent genes such as adhesion molecules and cyclooxygenase 2
Dahlman et al., J Biol Chem 2010 : Together, these data broaden our understanding of NF-kappaB during development by showing that in addition to its role as a negative regulator of myogenesis, NF-kappaB also regulates nitric-oxide synthase expression within stromal fibroblasts to stimulate myoblast fusion and muscle hypertrophy
Bahar-Shany et al., J Cell Physiol 2010 : Employing specific inhibitors we established that the induction of MMP-9 by TNFalpha was dependent on the activity of the epidermal growth factor receptor, c-Jun-N-terminal kinase (JNK), NFkappaB and extracellular signal regulated kinase-1/2
Moon et al., Cancer Lett 2010 (Leukemia) : Nuclear factor-kappa B (NF-kappaB) activation by tumor necrosis factor-alpha (TNF-alpha) attenuates the TNF-alpha induced apoptosis pathway ... Such sensitization is closely associated with the inhibitory effect of HA14-1 on TNF-alpha mediated NF-kappaB activation
Bui-Nguyen et al., J Biol Chem 2010 (Liver Neoplasms) : Pharmacological inhibition of the NF-kappaB signaling prevented the ability of HBx to stimulate the transcription, the expression, and the activity of iNOS ; nevertheless, these effects could be substantially rescued by MTA1 dysregulation
Punj et al., Oncogene 2010 (Sarcoma, Kaposi) : Our results show that K13 induced NF-kappaB activity suppresses CXCR4 through upregulation of miR-146a ... Our results show that K13 induced NF-kappaB activity suppresses CXCR4 through upregulation of miR-146a
Yang et al., Curr Cancer Drug Targets 2009 (Neoplasms) : In the second part, the mechanisms by which Gadd45 proteins are regulated by NF-kappaB , and how they affect MAPK activation, are reviewed
Chen et al., Exp Eye Res 2010 (Dry Eye Syndromes) : NF-kappaB inhibitor suppressed hyperosmoticity induced IL-1beta production ... p38 inhibitor suppressed hyperosmoticity induced NF-kappaB activation, indicating that NF-kappaB activation was dependent on p38 activation ... p38 inhibitor suppressed hyperosmoticity induced NF-kappaB activation, indicating that NF-kappaB activation was dependent on p38 activation
Gao et al., Regul Pept 2010 : Meprin-alpha also significantly increased LPS induced NF-kappaB transcriptional activity
Tanaka et al., Clin Exp Immunol 2010 (Acute Lung Injury) : Vitamin K3 also suppressed the lipopolysaccharide (LPS) induced nuclear translocation of NF-kappaB and production of TNF-alpha in mouse macrophage RAW264.7 cells ... In the ARDS model, vitamin K3 also suppressed the LPS induced increase in the serum TNF-alpha level and inhibited the LPS evoked nuclear translocation of NF-kappaB in lung tissue
Aiello et al., Bioorg Med Chem 2010 : The study evidenced one of the synthetic analogues ( 11 ) as a potent cytotoxic and pro-apoptotic agent against several tumor cell lines which also inhibits the TNFalpha induced NF-kappaB activation in a human leukemia T cell line
Kamal et al., Bioorg Med Chem 2010 : Therefore, the data generated suggests that these PBD conjugates activate p53 and inhibit NF-kappaB and thereby these compounds could be promising anticancer agents with better therapeutic potential for the suppression of tumours
Chengedza et al., Anticancer Drugs 2010 (Ovarian Neoplasms) : SHetA2 inhibited basal and TNF-alpha induced or hydrogen peroxide induced NF-kappaB activity through counter-regulation of upstream kinase ( IkappaB kinase ) activity, inhibitor protein ( IkappaB-alpha ) phosphorylation, and p-65 NF-kappaB subunit nuclear translocation, but independently of reactive oxygen species generation
Quiles-Perez et al., Hepatology 2010 (Carcinoma, Hepatocellular...) : Furthermore, the results obtained in mice treated with DEN to induce hepatocarcinogenesis showed, after treatment with a PARP inhibitor ( DPQ ), a significant reduction both in preneoplastic foci and in the expression of preneoplastic markers and proinflammatory genes ( Gstm3, Vegf, Spp1 [ Opn ], IL6, IL1b, and Tnf ), bromodeoxyuridine incorporation, and NF-kappaB activation in the initial steps of carcinogenesis ( P < 0.05 ) ... Furthermore, the results obtained in mice treated with DEN to induce hepatocarcinogenesis showed, after treatment with a PARP inhibitor ( DPQ ), a significant reduction both in preneoplastic foci and in the expression of preneoplastic markers and proinflammatory genes ( Gstm3, Vegf , Spp1 [ Opn ], IL6, IL1b, and Tnf ), bromodeoxyuridine incorporation, and NF-kappaB activation in the initial steps of carcinogenesis ( P < 0.05 ) ... Furthermore, the results obtained in mice treated with DEN to induce hepatocarcinogenesis showed, after treatment with a PARP inhibitor ( DPQ ), a significant reduction both in preneoplastic foci and in the expression of preneoplastic markers and proinflammatory genes ( Gstm3, Vegf, Spp1 [ Opn ], IL6, IL1b, and Tnf ), bromodeoxyuridine incorporation, and NF-kappaB activation in the initial steps of carcinogenesis ( P < 0.05 ) ... Furthermore, the results obtained in mice treated with DEN to induce hepatocarcinogenesis showed, after treatment with a PARP inhibitor ( DPQ ), a significant reduction both in preneoplastic foci and in the expression of preneoplastic markers and proinflammatory genes ( Gstm3 , Vegf, Spp1 [ Opn ], IL6, IL1b, and Tnf ), bromodeoxyuridine incorporation, and NF-kappaB activation in the initial steps of carcinogenesis ( P < 0.05 ) ... Furthermore, the results obtained in mice treated with DEN to induce hepatocarcinogenesis showed, after treatment with a PARP inhibitor ( DPQ ), a significant reduction both in preneoplastic foci and in the expression of preneoplastic markers and proinflammatory genes ( Gstm3, Vegf, Spp1 [ Opn ], IL6, IL1b , and Tnf ), bromodeoxyuridine incorporation, and NF-kappaB activation in the initial steps of carcinogenesis ( P < 0.05 ) ... Furthermore, the results obtained in mice treated with DEN to induce hepatocarcinogenesis showed, after treatment with a PARP inhibitor ( DPQ ), a significant reduction both in preneoplastic foci and in the expression of preneoplastic markers and proinflammatory genes ( Gstm3, Vegf, Spp1 [ Opn ], IL6 , IL1b, and Tnf ), bromodeoxyuridine incorporation, and NF-kappaB activation in the initial steps of carcinogenesis ( P < 0.05 )
Li et al., Am J Pathol 2010 (Brain Neoplasms...) : Bmi-1 activates NF-kappaB through stimulation of IkappaB phosphorylation, nuclear translocation, and transcriptional activity of NF-kappaB and expression of downstream genes of NF-kappaB including caspase-3, PARP, Bcl-X ( L ), and c-Myc
Kim et al., Biochem Biophys Res Commun 2010 (Neovascularization, Pathologic) : To address the mode of action of the compound, the effect of NBBA on TNF-alpha induced NF-kappaB transcription activity was investigated
Morais et al., Nephrol Dial Transplant 2010 (Carcinoma, Renal Cell...) : PDTC significantly decreased RCC growth and the expression of NF-kappaB subunits ( p50, p52, c-Rel and RelB ), upstream IKK-beta and IKK-gamma, but did not induce any changes in the expression of IkappaB-alpha and IkappaB-beta ... PDTC significantly decreased RCC growth and the expression of NF-kappaB subunits ( p50, p52, c-Rel and RelB ), upstream IKK-beta and IKK-gamma, but did not induce any changes in the expression of IkappaB-alpha and IkappaB-beta
de Zoete et al., Infect Immun 2010 (Campylobacter Infections) : In contrast, lysed bacteria induced strong NF-kappaB activation through human TLR1/2/6 and TLR4 and chicken TLR2t2/16 and TLR4 but not via TLR5 of either species ... In contrast, lysed bacteria induced strong NF-kappaB activation through human TLR1/2/6 and TLR4 and chicken TLR2t2/16 and TLR4 but not via TLR5 of either species ... In contrast, lysed bacteria induced strong NF-kappaB activation through human TLR1/2/6 and TLR4 and chicken TLR2t2/16 and TLR4 but not via TLR5 of either species
Rajaiya et al., Molecular vision 2009 (Adenoviridae Infections...) : Confocal microscopy showed that inhibitors of the p38, JNK, and ERK pathways differentially inhibited NFkappaB nuclear translocation, while PP2 , an inhibitor of Src family kinases, completely inhibited NFkappaB nuclear translocation ... Confocal microscopy showed that inhibitors of the p38 , JNK, and ERK pathways differentially inhibited NFkappaB nuclear translocation, while PP2, an inhibitor of Src family kinases, completely inhibited NFkappaB nuclear translocation
Amarilyo et al., Eur J Immunol 2010 : Using a luciferase reporter gene and measuring immune response to non-opsonic zymosan, we show that iC3b-apoptotic cells induce NF-kappaB inhibition in response to zymosan and LPS at the nuclear translocation, transcriptional and post-transcriptional levels, leading to profound inhibition of proinflammatory cytokines
Lee et al., Biochem Biophys Res Commun 2010 : alpha-iso-cubebenol also inhibited LPS induced nuclear translocation of the NF-kappaB p65 subunit
Dai et al., Biochem Biophys Res Commun 2010 (Myocarditis) : Myofibrillogenesis regulator-1(MR-1) can aggravate cardiac hypertrophy induced by angiotensin(Ang) II in mice through activation of NF-kappaB signaling pathway, and nuclear transcription factor (NF)-kappaB and activator protein-1(AP-1) regulate inflammatory and immune responses by increasing the expression of specific inflammatory genes in various tissues including heart
Tang et al., FEBS Lett 2010 (Neoplasms) : RKIP inhibits NF-kappaB in cancer cells by regulating upstream signaling components of the IkappaB kinase complex ... Since NF-kappaB target gene expression is subject to negative regulation involving the optimal induction of negative regulators, our data support a hypothesis that RKIP inhibits NF-kappaB activity via the auto-regulatory feedback loop by rapidly inducing the expression and synthesis of inhibitors of NF-kappaB activation
Merry et al., J Heart Lung Transplant 2010 (Lung Injury...) : Lungs were assessed for vascular permeability, myeloperoxidase content, bronchoalveolar lavage inflammatory cell and cytokine/chemokine content, as well as nuclear translocation of nuclear factor kappaB (NFkappaB) and activator protein-1 (AP-1), and interleukin-1 receptor associated kinase-1 ( IRAK-1 ) and stress activated protein kinase ( SAPK ) activation
Ichikawa et al., J Ethnopharmacol 2010 : The activation of NF-kappaB by TNF was completely blocked by a Lagerstroemia speciosa extract in a dose- and time dependent manner in H9c2 cells
Zaman et al., Vaccine 2010 : In the current study, we investigated the ability of our lipopeptides to activate nuclear factor-kappaB (NF-kappaB) in a toll-like receptor-2 (TLR2) dependent manner as the possible mode of action and reported the structure-function requirements for novel TLR2 targeting lipopeptides based on LAAs
Wang et al., J Biol Chem 2010 : In contrast to IRF3, ZAP induction was independent of NF-kappaB activity
Cho et al., J Cell Physiol 2010 (Calcinosis) : In this study, we determined the effect of TNF-alpha on osteogenic differentiation of stromal cells derived from human adipose tissue ( hADSC ) and the role of NF-kappaB activation on TNF-alpha activity ... TNF-alpha activated ERK and increased NF-kappaB promoter activity
Arevalo et al., J Neurochem 2009 : At 800 nM, Abeta ( 1-40 ) prevents NGF induced activation of NF-kappaB and consequently, it depresses the expression of Enhancer-of-split 1
Takamatsu et al., BMC microbiology 2010 (Legionnaires' Disease) : Inhibitors of NF-kappaB , p38 MAPK, and JNK blocked L. pneumophila induced IL-8 expression
Kazłowska et al., J Ethnopharmacol 2010 : Our results indicate that catechol and rutin, but not hesperidin, are primary bioactive phenolic compounds in the crude extract to suppress NO production in LPS stimulated macrophages via NF-kappaB dependent iNOS gene transcription
Kelly et al., J Pharmacol Exp Ther 2010 : CFTR ( inh ) -172, but not GlyH-101, induced nuclear translocation of nuclear factor-kappaB (NF-kappaB)
Ronaldson et al., Mol Pharmacol 2010 (MAP Kinase Signaling System) : Pharmacologic inhibition of nuclear factor-kappaB (NF-kappaB) signaling with SN50 prevented both TNF-alpha release and Mrp1 expression changes in astrocytes triggered with gp120 ; however, SN50 did not attenuate Mrp1 expression in cells triggered with TNF-alpha ... Furthermore, our results imply that both NF-kappaB and JNK signaling are involved in Mrp1 regulation during an HIV-1 associated inflammatory response
Wang et al., J Cell Biochem 2010 (Neoplasm Invasiveness...) : Consistent with these results, we found that the down-regulation of Notch-1 or Jagged-1 led to decreased expression and the activity of NF-kappaB downstream genes such as MMP-9, VEGF, and uPA, contributing to the inhibition of cell migration and invasion ... Consistent with these results, we found that the down-regulation of Notch-1 or Jagged-1 led to decreased expression and the activity of NF-kappaB downstream genes such as MMP-9, VEGF, and uPA, contributing to the inhibition of cell migration and invasion
Wu et al., J Cell Biochem 2010 : In this study, we demonstrate that NF-kappaB induces TAp63 gene expression ... The responsible elements for NF-kappaB mediated TAp63 induction are located within the region from -784 to -296 bp in the TAp63 promoter, which contains two NF-kappaB binding sites ... Inhibition of NF-kappaB by IkappaBalpha super-repressor or with a chemical inhibitor leads to down regulation of TAp63 mRNA expression and activity ... Activation of NF-kappaB by TNFalpha enhances p50/RelA binding to the NF-kappaB binding sites ... Activation of NF-kappaB by TNFalpha enhances p50/RelA binding to the NF-kappaB binding sites ... Furthermore, we show that an Sp1 site adjacent to the NF-kappaB sites plays a role in NF-kappaB mediated upregulation of TAp63
Morisugi et al., J Biochem 2010 : The interruption of NF-kappaB activating pathway by inhibitors resulted in the abrogation of those expressions, indicating the pivotal role of NF-kappaB in the mechanical stretch mediated COX-2 and iNOS expressions ... The interruption of NF-kappaB activating pathway by inhibitors resulted in the abrogation of those expressions, indicating the pivotal role of NF-kappaB in the mechanical stretch mediated COX-2 and iNOS expressions
Davis et al., Nature 2010 (Lymphoma, Large B-Cell, Diffuse) : The signalling adaptor CARD11 is required for constitutive NF-kappaB pathway activity and survival in ABC DLBCL
Park et al., The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2009 : HF6-FC exerts its inhibitory effects by suppression of p38 and NF-kappaB but activation of ERK
Kim et al., The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2009 : Since NF-kappaB is a major transcription factor that regulates genes for TLR2 and TNF-alpha, we examined the effect of rifampicin on the LPS induced NF-kappaB activation
Santos et al., Expert Opin Ther Targets 2010 (Inflammation...) : It suppresses p38 ( implicated in glucocorticoid-resistance ) and inhibits NF-kappaB activation, which has an important pathogenic role in several lung diseases
Zhang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2010 : Western blot results showed the NAC inhibited the IL-18 induced activation of NF-kappaB in VSMC
Ghosh-Choudhury et al., Cell Signal 2010 (Breast Neoplasms) : Furthermore, expression of NFkappaB p65 subunit inhibited transcription of PTEN , resulting in reduced protein expression, which leads to enhanced phosphorylation of Akt
Lluis et al., PloS one 2010 : As well as causing apoptosis of certain types of tumor cells, TRAIL can activate both NF-kappaB and JNK signalling pathways ... Overexpression of IKK2-EE, a constitutive activator of NF-kappaB, protected TAK1-/- MEFs against TRAIL killing, suggesting that TAK1 activation of NF-kappaB is critical for the viability of cells treated with TRAIL ... Consistent with this model, TRAIL failed to induce the survival genes cIAP2 and cFlipL in the absence of TAK1, whereas activation of NF-kappaB by IKK2-EE restored the levels of both proteins
Markwick et al., J Am Chem Soc 2010 : A biased-potential molecular dynamics simulation method, accelerated molecular dynamics ( AMD ), was combined with the chemical shift prediction algorithm SHIFTX to calculate ( 1 ) H ( N ), ( 15 ) N, ( 13 ) Calpha, ( 13 ) Cbeta, and ( 13 ) C ' chemical shifts of the ankyrin repeat protein IkappaBalpha ( residues 67-206 ), the primary inhibitor of nuclear factor kappa-B (NF-kappaB)
Long et al., Immunity 2009 : NF-kappaB directly promoted the transcription of Foxp3 , and upon T cell receptor ( TCR ) stimulation, c-Rel, a NF-kappaB family member, bound to Foxp3 enhancer region, which is specifically demethylated in natural Treg cells
Rosato et al., J Biol Chem 2010 (Leukemia) : LBH-589 triggered p65/RelA activation, NF-kappaB dependent induction of Mn-SOD2 , and ROS elimination ... LBH-589 triggered p65/RelA activation, NF-kappaB dependent induction of Mn-SOD2, and ROS elimination ... LBH-589 triggered p65/RelA activation, NF-kappaB dependent induction of Mn-SOD2, and ROS elimination ... Interference with LBH-589 mediated NF-kappaB activation ( e.g. in I kappaB alpha super-repressor transfected cells ) diminished HDACI mediated Mn-SOD2 induction and increased ROS accumulation, DNA damage, and apoptosis ... Interference with LBH-589 mediated NF-kappaB activation ( e.g. in I kappaB alpha super-repressor transfected cells ) diminished HDACI mediated Mn-SOD2 induction and increased ROS accumulation, DNA damage, and apoptosis ... The Mn-SOD2 mimetic TBAP ( manganese ( III ) -tetrakis 4-benzoic acid porphyrin ) prevented HDACI induced ROS and NF-kappaB activation while dramatically attenuating DNA damage and cell death
Sun et al., J Biol Chem 2010 : Here we report the identification of two regulatory phosphorylation sites at Thr-516 and Ser-520 within the kinase activation loop that is essential for MEKK3 mediated IkappaB kinase beta (IKKbeta)/NF-kappaB activation
Pantuck et al., Cancer Res 2010 (Carcinoma, Renal Cell...) : Based on earlier evidence that VHL loss can activate NF-kappaB , a known mediator of EMT, we tested whether NF-kappaB contributed to VHL mediated effects on EMT ... Based on earlier evidence that VHL loss can activate NF-kappaB, a known mediator of EMT, we tested whether NF-kappaB contributed to VHL mediated effects on EMT
Tubaro et al., J Cell Physiol 2010 : Conversely, silencing S100B expression in myoblast cell lines by RNA interference resulted in reduced NF-kappaB activity and enhanced MyoD, myogenin and MyHC expression and myotube formation
Stjernberg-Salmela et al., Rheumatology (Oxford) 2010 (Familial Mediterranean Fever) : Low TNF induced NF-kappaB and p38 phosphorylation levels in leucocytes in tumour necrosis factor receptor associated periodic syndrome ... Because the pathomechanism of TRAPS may involve aberrant TNF mediated intracellular signalling, we examined phosphorylation levels of nuclear factor kappaB (NF-kappaB) and p38 in response to TNF in 10 patients with three different TNFRSF1A mutations ( C73R, C88Y and F112I )
Palamarchuk et al., Blood 2010 (Leukemia, Lymphocytic, Chronic, B-Cell) : To examine the possible role of DLEU7 in CLL, we investigated the effect of DLEU7 expression on NF-kappaB and nuclear factor of activated T cells ( NFAT ) activity
Tanji et al., Blood 2010 : Twist augmented Tax mediated HTLV-1 LTR and NF-kappaB activation ... Twist augmented Tax mediated HTLV-1 LTR and NF-kappaB activation
Benard et al., PloS one 2010 (Colitis) : Degraded carrageenan causing colitis in rats induces TNF secretion and ICAM-1 upregulation in monocytes through NF-kappaB activation ... Degraded carrageenan causing colitis in rats induces TNF secretion and ICAM-1 upregulation in monocytes through NF-kappaB activation
Liu et al., Cancer Invest 2010 (Neovascularization, Pathologic) : The role of NF-kappaB in Hepatitis b virus X protein mediated upregulation of VEGF and MMPs ... This study investigated the role of NF-kappa B in HBx mediated upregulation of vascular endothelial growth factor ( VEGF ) and matrix metalloproteinases ( MMPs )
Oyesanya et al., Molecular cancer 2010 (Neoplasm Invasiveness) : In contrast, LPA stimulated another prominent transcription factor NF-kappaB via the Gq-PKC pathway in an EGFR independent manner ... In contrast, activation of G12/13, Gq and Gq-elicited NF-kappaB by LPA is independent of such an input
Dambrova et al., Int Immunopharmacol 2010 (Encephalitis) : ME10092 dose-dependently inhibited LPS induced nuclear translocation of NF-kappaB , transcription of tumour necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2)
Jeong et al., Bioconjug Chem 2010 : Upon activation of NFkappaB by interleukin-1beta (IL-1beta) , most of the plasmid distributed in the cytoplasm was localized within the nucleus, resulting in significantly higher gene transfection efficiency than controls with nondegradable PEI
Zhou et al., Int Immunopharmacol 2010 : Taken together, the present study demonstrated that nicotine induced COX-2 expression through NF-kappaB activation which mediated by nicotinic acetylcholine receptor and the induction of COX-2 was related to ICAM-1 expression
Rajendrasozhan et al., Pulm Pharmacol Ther 2010 : IkappaB kinase (IKK) activates NF-kappaB which plays a pivotal role in pro-inflammatory response in the lung ... The reduced pro-inflammatory response in PHA-408 administered rats was associated with decreased nuclear translocation and DNA binding activity of NF-kappaB in response to LPS or CS
Inokuchi et al., Proc Natl Acad Sci U S A 2010 (Inflammation...) : TNF-alpha increased caspase-3 activity but activated neither NF-kappaB nor JNK in Tak1-deficient hepatocytes
Park et al., Mol Cells 2010 (MAP Kinase Signaling System...) : Furthermore, as evidenced by MMP-9 promoter assays, melittin inhibited MMP-9 gene expression by blocking the PMA stimulated activations of activator protein-1 (AP-1) and nuclear factor-kappa B (NF-kappaB)
Yao et al., Inflamm Res 2010 : Moreover, NF-kappaB p50, COX-2, and iNOS levels in SF were markedly suppressed by AD-IL-18BP/IL-4 ... Moreover, NF-kappaB p50, COX-2, and iNOS levels in SF were markedly suppressed by AD-IL-18BP/IL-4 ... AD-IL-18BP/IL-4 can suppress the production and expression of inflammatory cytokines such as COX-2, iNOS, and NF-kappaB in LPS stimulated SF ... AD-IL-18BP/IL-4 can suppress the production and expression of inflammatory cytokines such as COX-2, iNOS, and NF-kappaB in LPS stimulated SF
Jin et al., Basic Clin Pharmacol Toxicol 2010 : MBMC also blocked LPS induced activation of nuclear factor kappaB (NF-kappaB) , p38 mitogen activated protein kinase and c-Jun N-terminal kinase (JNK)
Wang et al., Eur J Neurosci 2009 : These results suggest that overstimulation of NMDA receptors can induce NF-kappaB dependent expression of p53
Li et al., Zhongguo Wei Zhong Bing Ji Jiu Yi Xue 2010 : BLP stimulation resulted in dose dependent NF-KappaB activation in HEK293 cells stably expressing TLR2
Chou et al., Chem Biol Interact 2010 : We also found that EK5 markedly suppressed NF-kappaB signaling as detected by the NF-kappaB reporter gene assay but had no effects on the degradation of IkappaBalpha or translocation of NF-kappaB
Fu et al., Pain 2010 (Disease Models, Animal...) : As expected, following CCI nuclear translocation, and hence activation, of NF-kappaB was detected only in the sciatic nerve of wild type ( WT ) mice, and not in GFAP-IkappaBalpha-dn mice, while upregulation of GFAP was observed in the sciatic nerve and DRGs of both WT and GFAP-IkappaBalpha-dn mice, indicative of glial activation
Misra et al., Biochem Biophys Res Commun 2010 (Prostatic Neoplasms) : Here we report that treatment of prostate cancer cells with antibody directed against the carboxyl terminal domain of GRP78 inhibits alpha2M* induced activation of NF-kappaB2 by approximately 50 % while exerting a lesser effect of approximately 20 % on NF-kappaB1 activation
Sofronescu et al., Cardiovasc Res 2010 : FGF-16 is a target for adrenergic stimulation through NF-kappaB activation in postnatal cardiac cells and adult mouse heart
Mao et al., J Biol Chem 2010 : Here we show that knockdown of the E3 ubiquitin ligases cIAP1 and cIAP2 markedly inhibited virus triggered activation of IRF3 and NF-kappaB as well as IFN-beta induction
Jang et al., J Biol Chem 2010 (Anorexia) : In vitro, NF-kappaB activation directly stimulated the transcriptional activity of pro-opiomelanocortin (POMC), a precursor of anorexigenic melanocortin, and mediated the stimulatory effects of LPS, Tat , and pro-inflammatory cytokines on POMC transcription, implying the involvement of NF-kappaB in controlling feeding behavior ... In vitro, NF-kappaB activation directly stimulated the transcriptional activity of pro-opiomelanocortin (POMC), a precursor of anorexigenic melanocortin, and mediated the stimulatory effects of LPS , Tat, and pro-inflammatory cytokines on POMC transcription, implying the involvement of NF-kappaB in controlling feeding behavior ... In vitro, NF-kappaB activation directly stimulated the transcriptional activity of pro-opiomelanocortin (POMC) , a precursor of anorexigenic melanocortin, and mediated the stimulatory effects of LPS, Tat, and pro-inflammatory cytokines on POMC transcription, implying the involvement of NF-kappaB in controlling feeding behavior ... In addition, hypothalamic NF-kappaB was activated by leptin , an important anorexigenic hormone, and mediates leptin stimulated POMC transcription, indicating that hypothalamic NF-kappaB also serves as a downstream signaling pathway of leptin
Fenner et al., PloS one 2010 : Overexpression of CALB1, CDK2 and SAG was found to stimulate transcriptional activation by NF-kappaB, while SYT1 overexpression repressed TNFalpha dependent NF-kappaB transcriptional activation in human embryonic kidney cells ... Overexpression of CALB1, CDK2 and SAG was found to stimulate transcriptional activation by NF-kappaB, while SYT1 overexpression repressed TNFalpha dependent NF-kappaB transcriptional activation in human embryonic kidney cells ... Corresponding with this finding, RNA silencing of CDK2, SAG and SENP2 reduced NF-kappaB transcriptional activation, supporting a positive role for these proteins in the NF-kappaB pathway ... Corresponding with this finding, RNA silencing of CDK2 , SAG and SENP2 reduced NF-kappaB transcriptional activation, supporting a positive role for these proteins in the NF-kappaB pathway ... Corresponding with this finding, RNA silencing of CDK2, SAG and SENP2 reduced NF-kappaB transcriptional activation, supporting a positive role for these proteins in the NF-kappaB pathway
Lv et al., Inflamm Res 2010 (Inflammation) : Over-expression of caveolin-1 not only increased the production of pro-inflammatory cytokine TNF-alpha and IL-6, but also enhanced the expression of the cPLA2, p38 MAPK, and NF-kappaB
Shinozaki et al., Biochem Biophys Res Commun 2010 (Fibrosis...) : IL-13 inhibited the transcriptional activity of NF-kappaB , and the expression of mutant I-kappaB reproduced the suppression of autocrine TGF-beta(1) and promoted PSCs proliferation
Yang et al., Phytochemistry 2010 : The isolated compounds were evaluated for their cancer chemopreventive potential based on their ability to inhibit tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB activity, nitric oxide ( NO ) production, aromatase, quinone reductase 2 (QR2) and COX-1/-2 activities ... Palmatosides B ( 2 ) and C ( 3 ) inhibited TNF-alpha induced NF-kappaB activity with IC ( 50 ) values of 15.7 and 24.1 microM, respectively ; multiflorin A ( 4 ) inhibited aromatase enzyme with an IC ( 50 ) value of 15.5 microM ; afzelin ( 6 ) showed 68.3 % inhibition against QR2 at a concentration of 11.5 microg/ml ; palmatoside A ( 1 ) showed 52 % inhibition against COX-1 enzyme at a concentration of 10 microg/ml ; and multiflorin B ( 5 ) showed 52 % inhibition against nitric oxide production at a concentration of 20 microg/ml
Overman et al., Int J Obes Relat Metab Disord 2010 (Inflammation...) : We investigated the effect of GPE pretreatment on LPS mediated activation of mitogen activated protein kinases ( MAPKs ), nuclear factor kappa B (NF-kappaB) and activator protein-1 (AP-1), and induction of inflammatory genes in human MPhis ( that is, differentiated U937 cells ) ... Grape powder extract also attenuated LPS activation of MAPKs, NF-kappaB and AP-1 ( c-Jun ), as evidenced by decreased ( 1 ) phosphorylation of c-Jun NH ( 2 ) -terminal kinase ( JNK ) and p38 ; ( 2 ) degradation of IkappaBalpha and activation of an NF-kappaB reporter construct ; and ( 3 ) phosphorylation of c-Jun and Elk-1
Alciato et al., J Leukoc Biol 2010 : We also observed GSK3 beta phosphorylation and consequent inhibition of NF-kappaB nuclear translocation
Sousa et al., J Leukoc Biol 2010 (Inflammation) : Although LPS induced NF-kappaB activation, which was blocked by rolipram, NF-kappaB inhibitors did not promote resolution of neutrophil accumulation in this model
Mazzone et al., Biosci Trends 2009 (Atherosclerosis) : UCB prevented the nuclear translocation of NF-kappaB induced by TNFalpha
Hwang et al., Phytother Res 2010 : A further analysis indicated that EGJ attenuated TNF-alpha induced nuclear p65 nuclear factor-kappa B (NF-kappaB) translocation, suggesting that EGJ primarily affects the TNF-alpha induced NF-kappaB signaling pathway ... Taken together, we provided here the first evidence showing that EGJ is able to inhibit TNF-alpha induced NF-kappaB activation , adhesion molecule expression, and monocyte-endothelial interaction, suggesting an anti-inflammatory role of EGJ, which may be useful in preventing vascular diseases, such as atherosclerosis
Nomi et al., Invest Ophthalmol Vis Sci 2010 : The zymosan induced release of IL-6 and IL-8 was attenuated by inhibitors of ERK, p38, JNK, and NF-kappaB signaling ... The zymosan induced release of IL-6 and IL-8 was attenuated by inhibitors of ERK, p38, JNK, and NF-kappaB signaling
Zhao et al., Invest Ophthalmol Vis Sci 2010 (Graves Ophthalmopathy...) : Furthermore, the activation of NF-kappaB was significantly affected by ERK1/2 and JNK inhibitors, but not by p38 ... Furthermore, the activation of NF-kappaB was significantly affected by ERK1/2 and JNK inhibitors, but not by p38
Kim et al., Immune network 2009 : Stimulation of CD147 with its specific monoclonal antibody induced the expression of matrix metalloproteinase (MMP)-9 in THP-1 cells and it was suppressed by inhibitors of both ERK and NF-kappaB ... Stimulation of CD147 with its specific monoclonal antibody induced the expression of matrix metalloproteinase (MMP)-9 in THP-1 cells and it was suppressed by inhibitors of both ERK and NF-kappaB
Lee et al., Mol Cells 2010 (MAP Kinase Signaling System) : Furthermore, cell-permeable SOD inhibited LPS induced NF-kappaB DNA binding activity and activation of MAP kinases including ERK, JNK, and p38 in BV-2 cells ... Furthermore, cell-permeable SOD inhibited LPS induced NF-kappaB DNA binding activity and activation of MAP kinases including ERK, JNK, and p38 in BV-2 cells ... These data indicate that SOD has a regulatory function for LPS induced NF-kappaB activation leading to expression of iNOS and COX-2 in BV-2 cells and suggest that cell-permeable SOD is a feasible therapeutic agent for regulation of ROS related neurological diseases
Alonso et al., Cardiovasc Res 2010 (Disease Models, Animal...) : In the aortic SMC line of A7r5 cells, AngII activated kinase dependent pathways and induced binding of the nuclear factor-kappa B (NF-kappaB) to the Cx43 gene promoter, increasing Cx43 expression
Lu et al., J Biol Chem 2010 : Thus, CTCF by itself plays a central role in mediating the dichotomous effects of growth factor- and stress stimulated NF-kappaB activation on cell survival and death
Roccaro et al., Blood 2010 (Lymphoma...) : Primary WM cells express higher level of i20S compared with c20S, and that ONX0912 inhibited the CT-L activity of both i20S and c20S, leading to induction of toxicity in primary WM cells, as well as of apoptosis through c-Jun N-terminal kinase activation, nuclear factor kappaB (NF-kappaB) inhibition , caspase cleavage, and initiation of the unfolded protein response ... Primary WM cells express higher level of i20S compared with c20S, and that ONX0912 inhibited the CT-L activity of both i20S and c20S, leading to induction of toxicity in primary WM cells, as well as of apoptosis through c-Jun N-terminal kinase activation, nuclear factor kappaB (NF-kappaB) inhibition , caspase cleavage, and initiation of the unfolded protein response
Kang et al., Molecules (Basel, Switzerland) 2010 : Furthermore, luteolin suppressed the expression of TNF-alpha, IL-8, IL-6, GM-CSF, and COX-2 through a decrease in the intracellular Ca2+ levels, and also showed a suppression of the ERK 1/2, JNK 1/2, and NF-kappaB activation
Martins et al., PloS one 2010 (Salmonella Infections) : This anti-inflammatory effect was correlated with an inhibitory effect of Sb on ST-induced activation of the MAPKs ERK1/2, p38 and JNK as well as on activation of NF-kappaB ... This anti-inflammatory effect was correlated with an inhibitory effect of Sb on ST-induced activation of the MAPKs ERK1/2 , p38 and JNK as well as on activation of NF-kappaB
Kalliolias et al., Arthritis Rheum 2010 (Arthritis, Rheumatoid...) : IL-27 inhibited human osteoclastogenesis, suppressed the induction of NFATc1 , down-regulated the expression of RANK and triggering receptor expressed on myeloid cells 2 ( TREM-2 ), and inhibited RANKL mediated activation of ERK, p38, and NF-kappaB in osteoclast precursors ... IL-27 inhibited human osteoclastogenesis, suppressed the induction of NFATc1, down-regulated the expression of RANK and triggering receptor expressed on myeloid cells 2 ( TREM-2 ), and inhibited RANKL mediated activation of ERK, p38, and NF-kappaB in osteoclast precursors ... IL-27 inhibited human osteoclastogenesis, suppressed the induction of NFATc1, down-regulated the expression of RANK and triggering receptor expressed on myeloid cells 2 ( TREM-2 ), and inhibited RANKL mediated activation of ERK, p38, and NF-kappaB in osteoclast precursors
Chen et al., Molecular cancer 2010 (Carcinoma, Non-Small-Cell Lung...) : The combination of GGTI-298 and TRAIL was more effective than each single agent in decreasing the levels of IkappaBalpha and p-Akt, implying that GGTI298/TRAIL activates NF-kappaB and inhibits Akt ... The combination of GGTI-298 and TRAIL was more effective than each single agent in decreasing the levels of IkappaBalpha and p-Akt, implying that GGTI298/TRAIL activates NF-kappaB and inhibits Akt
Scotto d'Abusco et al., Arthritis Res Ther 2010 : Inhibitor kappaB kinase (IKK)alpha and IKKbeta are kinases involved in the activation of the NF-kappaB transcription factor ... Inhibitor kappaB kinase (IKK)alpha and IKKbeta are kinases involved in the activation of the NF-kappaB transcription factor
Park et al., Toxicol In Vitro 2010 (Carcinoma, Squamous Cell...) : These findings collectively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activation of caspases, caspase dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT , and activation of NF-kappaB ... These findings collectively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activation of caspases, caspase dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of NF-kappaB ... These findings collectively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activation of caspases, caspase dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of NF-kappaB ... These findings collectively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activation of caspases, caspase dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of NF-kappaB ... These findings collectively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activation of caspases, caspase dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of NF-kappaB
Kunze et al., Prostaglandins Other Lipid Mediat 2010 (Inflammation) : C4S inhibited the enhanced expression of COX-2 and mPGES1 but had no effect on the IL1beta induced decrease of I-kappaBalpha and nuclear translocation of NF-kappaB
Karlsen et al., Eur J Nutr 2010 (Cardiovascular Diseases) : Furthermore, we studied the effect of polyphenols from bilberries on lipopolysaccharide (LPS) induced NF-kappaB activation in a monocytic cell line
Wang et al., Chin Med Sci J 2009 : The inhibition of phosphorylated Akt by LY294002 led to the inhibition of NF-kappaB in a dose dependent manner ... S. aureus induces apoptosis of U937 cells by inhibiting Akt regulated NF-kappaB
Boucher et al., Viral Immunol 2010 (HIV Infections...) : In THP-1 cells, p38 , ERK, and JNK inhibition increased NF-kappaB and Sp1 binding to the IL-12p40 promoter, while inhibiting AP-1 binding ... In THP-1 cells, p38, ERK , and JNK inhibition increased NF-kappaB and Sp1 binding to the IL-12p40 promoter, while inhibiting AP-1 binding
Fu et al., J Exp Med 2010 (Autoimmune Diseases...) : We demonstrate that PLCgamma1 deficiency affects positive and negative selection, significantly reduces single positive thymocytes and peripheral T cells, and impairs TCR induced proliferation and cytokine production, and the activation of ERK, JNK, AP-1, NFAT, and NF-kappaB
Nadler et al., PLoS Pathog 2010 (Escherichia coli Infections) : Importantly, we show that NleE inhibits NF-kappaB activation by preventing activation of IKKbeta and consequently the degradation of the NF-kappaB inhibitor, IkappaB
Sanz et al., PloS one 2010 : We now explore TWEAK induced activation of NFkappaB2 and RelB, as well as expression of CCL21, a T-cell chemotactic factor, in cultured murine tubular epithelial cells and in healthy kidneys in vivo ... In cultured tubular cells, TWEAK and TNFalpha activated different DNA binding NFkappaB complexes ... In cultured tubular cells, TWEAK and TNFalpha activated different DNA binding NFkappaB complexes ... In vivo, TWEAK induced nuclear NFkappaB2 and RelB translocation and CCL21a mRNA ( 1.5+/-0.3-fold over control ) and CCL21 protein ( 1.6+/-0.5-fold over control ) expression in normal kidney
Xie et al., Oncol Rep 2010 (Glioblastoma...) : Constitutive NF-kappaB activity regulates the expression of VEGF and IL-8 and tumor angiogenesis of human glioblastoma ... Constitutive NF-kappaB activity regulates the expression of VEGF and IL-8 and tumor angiogenesis of human glioblastoma
Leitch et al., Eur J Immunol 2010 : This effect did not appear to be mediated via effects on early markers of neutrophil activation ( e.g. surface marker expression, shape change, aggregation and superoxide anion generation ), by direct inhibition of NF-kappaB activation ( assessed by cytoplasmic IkappaBalpha proteolysis and NF-kappaB p65 subunit translocation ) and ERK activation ( determined by specific ERK phosphorylation ) but due to down-regulation ( at protein and mRNA level ) of the survival protein Mcl-1 but not the pro-apoptotic bcl-2 homologue Bim
Jung et al., Br J Pharmacol 2010 : We examined the effects of cilostazol on LPS induced nuclear factor-kappaB (NF-kappaB) activation and phosphorylation of mitogen activated protein kinases ( MAPKs )
Mehrotra et al., Cancer Cell 2010 (Neoplasm Invasiveness) : Instead, a survivin-XIAP complex activates NF-kappaB , which in turn leads to increased fibronectin gene expression, signaling by beta1 integrins, and activation of cell motility kinases FAK and Src
Khan et al., J Immunol 2010 : Conversely, stable overexpression of Sigirr diminished NF-kappaB mediated IL-8 responses to TLR ligands
Wu et al., Gynecol Obstet Invest 2010 (Endometriosis) : To determine whether nuclear factor-kappaB (NF-kappaB) is constitutively and tumor necrosis factor (TNF)-dependently activated in endometriotic cells, whether trichostatin A (TSA) can suppress NF-kappaB activation and suppress TRAF2/6 and TAK1, and whether TSA and caffeic acid phenyl ester can suppress constitutive and H ( 2 ) O ( 2 ) -stimulated proliferation of endometriotic cells ... Electrophoretic mobility shift analysis was used to determine NF-kappaB activation and possible suppression by TSA ... TNFalpha stimulation activated NF-kappaB through induction of IkappaB phosphorylation, but the activation can be suppressed by TSA ... TNFalpha stimulation activated NF-kappaB through induction of IkappaB phosphorylation, but the activation can be suppressed by TSA
Taylor et al., Stem Cells Dev 2010 : Toll-like receptor 2 mediates proliferation, survival, NF-kappaB translocation, and cytokine mRNA expression in LIF maintained mouse embryonic stem cells
Luecke et al., Chem Biol Interact 2010 : Now, our findings clearly indicate a role of NFkappaB also in UVB dependent AhR signaling ... Together, our results suggest that the NFkappaB mediated initial suppression of CYP1A1 as well as the unresponsiveness of AhR signaling to repeated irradiation may be part of a protective cellular UV stress response
Chen et al., Chem Biol Interact 2010 : Nitrosative stress induces osteoblast apoptosis through downregulating MAPK mediated NFkappaB/AP-1 activation and subsequent Bcl-X ( L ) expression
Siggs et al., Proc Natl Acad Sci U S A 2010 (Immunologic Deficiency Syndromes) : Degradation of IkappaB alpha , which is considered a primary requirement for NEMO mediated immune signaling, occurred normally in response to Toll-like receptor stimulation, yet ERK phosphorylation and NF-kappaB p65 nuclear translocation were severely impaired
Manna et al., J Biol Chem 2010 : In this report, we provide evidence that P(3)-25 inhibits NF-kappaB , known to induce inflammatory and tumorigenic responses ... It inhibits TRAF2 mediated NF-kappaB activation but not TRAF6 mediated NF-kappaB DNA binding by preventing its association with TANK ( TRAF for NF-kappaB ) ... It inhibits TRAF2 mediated NF-kappaB activation but not TRAF6 mediated NF-kappaB DNA binding by preventing its association with TANK ( TRAF for NF-kappaB ) ... It inhibits TRAF2 mediated NF-kappaB activation but not TRAF6 mediated NF-kappaB DNA binding by preventing its association with TANK ( TRAF for NF-kappaB )
Lerner et al., J Physiol Pharmacol 2009 : Inhibition of NF-kappaB with curcumin or parthenolide resulted in a decrease of IL-8 secretion
Tsou et al., J Cell Biochem 2010 : TNF-alpha mediated an increase of NF-kappaB-specific DNA-protein complex formation, p65 translocation into nucleus, NF-kappaB-luciferase activity was inhibited by KP392, Akt inhibitor, and rapamycin
Sugioka et al., J Orthop Res 2010 : The transfection rate was assessed by counting FITC positive cells, and IL-1 induced NF-kappaB activation in the cells was assessed ... IL-1 induced NF-kappaB activation was significantly inhibited by pretreatment of the cells with NF-kappaB decoy combined with radial shock wave exposure
Seo et al., Curr Eye Res 2010 (Dacryocystitis...) : Furthermore, DA-6034 attenuated the phosphorylation of JNK and p38 MAPK and inhibited NF-kB activation in a concentration dependent manner in corneal epithelial cells
Liang et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2010 : The expression levels of caspase-3 and bax after co-treatment of GA and Fe3O4-MNP were higher than that exposed to GA or Fe3O4-MNP alone, but the expressions of bcl-2, NF-kappaB and survivin were down-regulated
Ravindran et al., Biochem Pharmacol 2010 : We found that bisdemethylcurcumin ( BDC ) was more potent than curcumin as an anti-inflammatory agent as indicated by suppression of TNF induced NF-kappaB activation, more potent as an anti-proliferative agent, and more potent in inducing ROS
Lee et al., Int Immunopharmacol 2010 (Dermatitis) : These findings suggest that hirsutenone may exert a preventive effect against microbial endotoxin lipopolysaccharide induced inflammatory skin diseases through inhibition of ERK pathway mediated NF-kappaB activation
Ikeda et al., FEBS Lett 2010 : BS69 cooperates with TRAF3 in the regulation of Epstein-Barr virus derived LMP1/CTAR1 induced NF-kappaB activation ... Previous studies have demonstrated that BS69, a multidomain cellular protein, regulates LMP1/CTAR2 mediated NF-kappaB activation by interfering with the complex formation between TRADD and LMP1/CTAR2 ... Here, we found that BS69 directly interacted with the LMP1/CTAR1 domain and regulated LMP1/CTAR1 mediated NF-kappaB activation and subsequent IL-6 production ... Regarding the mechanisms involved, we found that BS69 directly interacted with TRAF3 , a negative regulator of NF-kappaB activation ... Furthermore, small interfering RNA mediated knockdown experiments revealed that TRAF3 was involved in the BS69 mediated suppression of LMP1/CTAR1 induced NF-kappaB activation ... Furthermore, small interfering RNA mediated knockdown experiments revealed that TRAF3 was involved in the BS69 mediated suppression of LMP1/CTAR1 induced NF-kappaB activation
Yang et al., Atherosclerosis 2010 : In cultured human aortic endothelial cells, TNF-alpha increased ET-1 through AP-1 and NF-kappaB , whereas MPA attenuated it by reducing both AP-1 and NF-kappaB DNA binding activities ... N-acetylcysteine, SP600125 ( JNK inhibitor ) and SB203580 ( p38 MAPK inhibitor ) attenuated TNF-alpha induced DNA binding activities of both AP-1 and NF-kappaB ... N-acetylcysteine, SP600125 ( JNK inhibitor ) and SB203580 ( p38 MAPK inhibitor ) attenuated TNF-alpha induced DNA binding activities of both AP-1 and NF-kappaB ... N-acetylcysteine, SP600125 ( JNK inhibitor ) and SB203580 ( p38 MAPK inhibitor ) attenuated TNF-alpha induced DNA binding activities of both AP-1 and NF-kappaB
Boyanovsky et al., Cytokine 2010 : Indirect immunofluorescence showed that GV-LDL but not control-LDL induced nuclear translocation of NFkappaB ... Inhibitors of NFkappaB activation, effectively blocked cytokine production induced by GV-LDL
Lee et al., Toxicol Lett 2010 (Carcinoma, Hepatocellular...) : Hesperidin suppressed TPA stimulated NF-kappaB translocation into the nucleus through IkappaB inhibitory signaling pathways and also inhibited TPA induced AP-1 activity by the inhibitory phosphorylation of p38 kinase and c-Jun N-terminal kinase (JNK) signaling pathways
Xie et al., J Immunol 2010 : Knockdown of TNFR associated factor 6 or NF-kappaB activator 1 in 70Z/3 pre-B cells led to decreased Rgs16 expression, indicating that both of these two genes are involved in IL-17 mediated activation of NF-kappaB signaling in B cells
López-Huertas et al., Nucleic Acids Res 2010 : First, NF-kappaB- , NF-AT- and Sp1 dependent transcriptional activities were greatly enhanced in Jurkat-Tat101, whereas Tat72 induced milder but efficient activation
Satake et al., Zoolog Sci 2010 : Furthermore, both Ci-TLR1 and Ci-TLR2 stimulate NF-kappaB induction in response to multiple pathogenic ligands that are differentially recognized by respective vertebrate TLRs. Pathogenic ligands that stimulate the Ci-TLRs also induce the expression of Ci-TNFalpha in the intestine and stomach, where the Ci-TLRs are abundantly expressed ... Furthermore, both Ci-TLR1 and Ci-TLR2 stimulate NF-kappaB induction in response to multiple pathogenic ligands that are differentially recognized by respective vertebrate TLRs. Pathogenic ligands that stimulate the Ci-TLRs also induce the expression of Ci-TNFalpha in the intestine and stomach, where the Ci-TLRs are abundantly expressed
Rahman et al., J Cell Mol Med 2009 (Osteoporosis) : LPS stimulated COX-II activity as well as NF-kappaB activation in BM cells from Fat-1 Ovx mice was significantly less as compared to BM cells from WT Ovx mice
Hommelberg et al., Lipids 2010 (Insulin Resistance) : Of all tested unsaturated FAs, only elaidic acid ( 3-fold ), cis9,trans11-CLA ( 3-fold ) and trans10,cis12-CLA ( 13-fold ) increased NF-kappaB transactivation in myotubes
Zhou et al., Nucleic Acids Res 2010 : Thus, a subset of miRNA genes is regulated in human biliary epithelial cells through NF-kappaB activation induced by LPS , suggesting a role of the NF-kappaB pathway in the transcriptional regulation of miRNA genes
Qu et al., Cancer Res 2010 (Colorectal Neoplasms) : Indeed, expression of exogenous PDLIM2 inhibited constitutive NF-kappaB activation in these colorectal cancer cells
Okada et al., J Vet Med Sci 2010 : IL-1beta ( 4 ng/ml, 10 min ) induced phosphorylation of NF-kappaB , JNK, and ERK ... Angiotensin II augmented the IL-1beta induced phosphorylation of ERK but not NF-kappaB and JNK
Diel et al., J Virol 2010 (Ecthyma, Contagious) : Expression of ORFV024 in cell cultures significantly decreased lipopolysaccharide (LPS)- and tumor necrosis factor alpha (TNF-alpha) induced NF-kappaB-responsive reporter gene expression ... Further, ORFV024 expression decreased TNF-alpha induced phosphorylation and nuclear translocation of NF-kappaB-p65 , phosphorylation, and degradation of IkappaBalpha, and phosphorylation of IkappaB kinase (IKK) subunits IKKalpha and IKKbeta, indicating that ORFV024 functions by inhibiting activation of IKKs, the bottleneck for most NF-kappaB activating stimuli
Chan et al., Circ Res 2010 : Furthermore, increased NFkappaB expression after injury transactivated the TRAIL promoter ... Conclusions : We conclude that TRAIL induction involves FGF-2, Sp1-phosphorylation and NFkappaB and that TRAIL promotes VSMC proliferation and neointima formation after arterial injury
Teng et al., Mediators Inflamm 2009 (Cerebral Hemorrhage) : Activation of TLR4 mediated NFkappaB signaling in hemorrhagic brain in rats ... However, the involvement of TLR4 mediated NFkappaB signaling in the pathogenesis of ICH remains unknown ... The present study was to evaluate the temporal profile of the expression of TLR4 and the activation of TLR4 mediated NFkappaB signaling in brain tissues of Wistar rats after ICH ... The present study firstly suggests that TLR4 mediated NFkappaB signaling participates in the pathogenesis of ICH, which may become a therapeutic target for ICH induced brain damage
Funakoshi-Tago et al., Int Immunopharmacol 2010 (Edema) : Previously, we reported that a major component, Licochalcone A, potently inhibited TNFalpha induced NF-kappaB activation by inhibiting IKKbeta activation ... Previously, we reported that a major component, Licochalcone A, potently inhibited TNFalpha induced NF-kappaB activation by inhibiting IKKbeta activation ... Interestingly, reduced Licochalcone A, which lacks a double bond, failed to inhibit TNFalpha induced NF-kappaB activation ... Whereas Licochalcone A potently inhibited TNFalpha induced IKK activation, IkappaBalpha degradation, nuclear localization of NF-kappaB and its DNA binding activity, no inhibitory effect was observed by reduced Licochalcone A
Nehra et al., FASEB J 2010 (Breast Neoplasms) : BCL2 and CASP8 regulation by NF-kappaB differentially affect mitochondrial function and cell fate in antiestrogen-sensitive and -resistant breast cancer cells ... NF-kappaB inhibition restores the ability of 4HT to decrease BCL2 expression, increase mitochondrial membrane permeability, and induce a caspase dependent apoptotic cell death in resistant cells
Min et al., Nat Med 2010 (Neoplasm Invasiveness...) : Moreover, we report that epigenetic silencing of DAB2IP is a key mechanism by which the polycomb-group protein histone-lysine N-methyltransferase EZH2 activates Ras and NF-kappaB and triggers metastasis
Youn et al., Nutr Cancer 2009 (Colitis...) : Administration of DSS ( 2.5 % ) in drinking water for 7 days to male ICR mice resulted in colitis and elevated expression of inducible nitric oxide synthase (iNOS) and activation of nuclear factor-kappa B (NF-kappaB) , a major transcription factor known to upregulate proinflammatory gene expression ... Oral administration of resveratrol or piceatannol ( 10 mg/kg body weight each ) for 7 constitutive days attenuated the DSS induced inflammatory injury, upregulation of iNOS expression, and activation of NF-kappaB , STAT3, and ERK ... Oral administration of resveratrol or piceatannol ( 10 mg/kg body weight each ) for 7 constitutive days attenuated the DSS induced inflammatory injury, upregulation of iNOS expression, and activation of NF-kappaB , STAT3, and ERK
Chuang et al., J Neurosci Res 2010 (Disease Models, Animal...) : We conclude that activation of NF-kappaB in hippocampal CA3 neurons upregulates NOS II gene expression following experimental temporal lobe status epilepticus, leading to apoptotic neuronal cell death in the hippocampus
Kim et al., J Mol Biol 2010 (Fatty Liver) : HBx enhanced transcriptional activities of SREBP1 and PPAR gamma are significantly attenuated by the NF-kappaB inhibitor Bay 11-7082, as well as by TNFR1 depletion ... HBx enhanced transcriptional activities of SREBP1 and PPAR gamma are significantly attenuated by the NF-kappaB inhibitor Bay 11-7082, as well as by TNFR1 depletion ... Our results suggest that HBx induced NF-kappaB activation was mediated by direct interaction with TNFR1 and thereby induced TNF-alpha production ... Our results suggest that HBx induced NF-kappaB activation was mediated by direct interaction with TNFR1 and thereby induced TNF-alpha production
Oh et al., Int Immunopharmacol 2010 : BAI inhibited nuclear factor-kappaB (NF-kappaB) activity and nuclear translocation of NF-kappaB
Ko et al., Eur J Pharmacol 2010 : Furthermore, inflexin inhibited the degradation of IkappaB-alpha and the activation of NF-kappaB , p65 and Akt, while the MAPKs signal pathway was not affected
Trinh et al., Arch Pharm Res 2009 (Pruritus) : These curcuminoids also inhibited degranulation, protein expression of TNF-alpha and IL-4, and transcription factor NF-kappaB activation in IAC induced RBL-2H3 cells ... These curcuminoids also inhibited degranulation, protein expression of TNF-alpha and IL-4 , and transcription factor NF-kappaB activation in IAC induced RBL-2H3 cells
Srivastava et al., J Biol Chem 2010 : NF-kappaB activation in T cells requires discrete control of IkappaB kinase alpha/beta ( IKKalpha/beta ) phosphorylation and IKKgamma ubiquitination by the ADAP adapter protein ... NF-kappaB activation in T cells requires discrete control of IkappaB kinase alpha/beta ( IKKalpha/beta ) phosphorylation and IKKgamma ubiquitination by the ADAP adapter protein ... Although both the TAK1 and the CARMA1 binding sites in ADAP are essential for IkappaB alpha phosphorylation and degradation and NF-kappaB nuclear translocation, only the TAK1 binding site in ADAP is necessary for IKK phosphorylation
Zhou et al., J Biol Chem 2010 (Hepatitis B, Chronic) : As a consequence, up-regulation of IP-10 may mediate the migration of peripheral blood leukocytes in a NF-kappaB dependent manner
Bai et al., J Immunol 2010 : CCN1 regulates this genetic program through at least two distinct mechanisms : an immediate-early response resulting from direct activation of NF-kappaB by CCN1 , leading to the synthesis of cytokines including TNF-alpha and inflammatory protein 10 ; and a delayed response resulting from CCN1 induced TNF-alpha, which acts as an autocrine/paracrine mediator to activate the expression of other cytokines including IL-1beta and IL-6
Adamopoulos et al., Arthritis Res Ther 2010 : Interleukin-17A upregulates receptor activator of NF-kappaB on osteoclast precursors ... IL-17A upregulates the receptor activator for NF-kappaB receptor on human osteoclast precursors in vitro, leading to increased sensitivity to RANKL signalling, osteoclast differentiation and bone loss
Lee et al., Am J Pathol 2010 (Inflammation) : LTA stimulated NF-kappaB translocation or cPLA(2) phosphorylation was attenuated by pretreatment with LY294002, SB202190, U0126, or SP600125
Marquez et al., Am J Physiol Gastrointest Liver Physiol 2010 : MicroRNA-21 is upregulated during the proliferative phase of liver regeneration, targets Pellino-1 , and inhibits NF-kappaB signaling ... It has been previously reported that NF-kappaB upregulates the miR-21 precursor transcript ... Using NF-kappaB reporter assays, we determined that miR-21 overexpression inhibits NF-kappaB signaling
Wang et al., Circ Res 2010 : Analysis of aortic endothelial cells from AMPKalpha2 ( -/- ) mice and human umbilical vein endothelial cells expressing dominant negative AMPK or AMPKalpha2-specific siRNA revealed that loss of AMPK activity increased NAD ( P ) H oxidase subunit expression ( gp91(phox), p47(phox), p67(phox), NOX1 and -4 ), NAD ( P ) H oxidase mediated superoxide production, 26S proteasome activity, IkappaBalpha degradation, and nuclear translocation of NF-kappaB ( p50 and p65 ), whereas AMPK activation by AICAR or overexpression of constitutively active AMPK had the opposite effect ... Consistently, we found that genetic deletion of AMPKalpha2 in low-density lipoprotein receptor knockout ( LDLr ( -/- ) ) strain markedly increased 26S proteasome activity, IkappaB degradation, NF-kappaB transactivation , NAD ( P ) H oxidase subunit overexpression, oxidative stress, and endothelial dysfunction, all of which were largely suppressed by chronic administration of MG132, a potent cell permeable proteasome inhibitor ... Consistently, we found that genetic deletion of AMPKalpha2 in low-density lipoprotein receptor knockout ( LDLr ( -/- ) ) strain markedly increased 26S proteasome activity, IkappaB degradation, NF-kappaB transactivation , NAD ( P ) H oxidase subunit overexpression, oxidative stress, and endothelial dysfunction, all of which were largely suppressed by chronic administration of MG132, a potent cell permeable proteasome inhibitor
Maioli et al., J Biomed Biotechnol 2009 : We also demonstrate that Rottlerin prevents TNFalpha dependent NFkappaB activation in MCF-7 cells and in HT-29 cells transfected with the NFkappaB-driven plasmid pBIIX-LUC, suggesting that Rottlerin can inhibit NFkappaB via several pathways and in several cell types
Lin et al., Virology 2010 (Inflammation...) : ARV S1133 activated PI 3-kinase dependent Akt/NF-kappaB and p70 S6 kinase, as well as Stat3 ; however, p70 S6 kinase was not involved in ARV S1133 mediated effects
Lin et al., Cytokine 2010 : We next revealed that PKR activity mediates LMP1 enhanced NF-kappaB nuclear translocation resulting in cytokine induction ... Our findings thus suggest that PKR plays an important role in mediating the cytokine gene expression induced by LMP1 through NF-kappaB activation and histone H3 Ser 10 phosphorylation
You et al., Mol Cell Endocrinol 2010 : These findings suggest that PLC/CAMK IV-NF-kappaB is involved in RAGE mediated signaling pathway in human endothelial cells
Privratsky et al., J Immunol 2010 : To confirm and extend these observations, we examined the effect of engaging, cross linking, or expressing PECAM-1 on NF-kappaB activation in a variety of human cells
Hirao et al., Life Sci 2010 (Inflammation) : In TLR2 ligand stimulated HDPF, specific inhibitors of extracellular signal regulated kinase ( ERK ) 1/2, p38, c-jun NH ( 2 ) -terminal kinase ( SAP/JNK ), NF-kappaB or catechins markedly reduced the level of pro-inflammatory mediators and the phosphorylation of these signal transduction molecules was suppressed by catechins
Sheng et al., J Immunol 2010 : ROS ( hi ) DCs could not initiate and sustain a significant level of NF-kappaB phosphorylation in response to LPS and zymosan, although demonstrating hyperactivation of p38 MAPK by LPS, in a fashion disparate to ROS ( lo ) DCs
Bansal et al., J Immunol 2010 (Tuberculosis) : We further found that PE_PGRS protein mediated activation of DCs involves participation of ERK1/2, p38 MAPK, and NF-kappaB signaling pathways
Marr et al., Infect Immun 2010 : We compared purified endotoxin and heat killed B. pertussis BP338 whole cells that have modified lipid A phosphate groups to an isogenic mutant lacking this modification with respect to their capacities to induce the release of inflammatory cytokines by human and murine macrophages and to participate in the TLR4 mediated activation of NF-kappaB in transfected HEK-293 cells
Aoki et al., J Atheroscler Thromb 2010 : Cilostazol dose-dependently inhibited LPS induced NF-kappaB activation in the present study
Cui et al., Cancer Sci 2010 (Breast Neoplasms...) : Taken together, these observations suggest that Shh overexpression is a critical event in breast carcinogenesis, and Shh promoter hypomethylation and NF-kappaB up-regulation are responsible for the up-regulation of Shh
Lee et al., Journal of inflammation (London, England) 2010 : DSS and TNBS induced the protein expression of TLR-4 and activated transcription factor NF-kappaB ... IL-1beta potently induced NF-kappaB activation in CaCo-2 cells, but did not induce TLR-4 expression ... IL-1beta potently induced NF-kappaB activation in CaCo-2 cells, but did not induce TLR-4 expression
Nishiumi et al., Toxicological sciences : an official journal of the Society of Toxicology 2010 (Insulin Resistance) : TCDD also stimulated the phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2 and c-Jun N-terminal kinase (JNK), and their inhibitors abrogated the TCDD induced downregulation of IRbeta, IRS1, and GLUT4 ; upregulation of TNF-alpha ; and activation of NF-kappaB ... TCDD also stimulated the phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2 and c-Jun N-terminal kinase (JNK), and their inhibitors abrogated the TCDD induced downregulation of IRbeta, IRS1, and GLUT4 ; upregulation of TNF-alpha ; and activation of NF-kappaB ... TCDD also stimulated the phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2 and c-Jun N-terminal kinase (JNK), and their inhibitors abrogated the TCDD induced downregulation of IRbeta, IRS1 , and GLUT4 ; upregulation of TNF-alpha ; and activation of NF-kappaB
Mattaliano et al., Am J Physiol Cell Physiol 2010 : In addition, OxLDL treatment stimulated ROCK2 catalytic activity, and ROCK2 inhibition attenuated NF-kappaB activation and IL-8 production resulting from OxLDL activation of LOX-1
Wang et al., FASEB J 2010 (Hypercapnia) : In contrast, hypercapnia did not down-regulate IL-10 or interferon-beta, which do not require NF-kappaB
Kaus et al., Stem Cells Dev 2010 : Neural stem cells adopt tumorigenic properties by constitutively activated NF-kappaB and subsequent VEGF up-regulation
Kuphal et al., Eur J Cancer 2010 (Melanoma...) : Interestingly, the inhibition of the NFkappaB pathway also reduced the accumulation of HIF-1alpha assuming a context between ROS and NFkappaB, and suggesting that ROS and NFkappaB activity contribute to HIF-1alpha accumulation
Bista et al., J Biol Chem 2010 : TRAF3 controls activation of the canonical and alternative NFkappaB by the lymphotoxin beta receptor ... TRAF3 controls activation of the canonical and alternative NFkappaB by the lymphotoxin beta receptor ... Analysis of NFkappaB signaling in cell lines with functionally intact NFkappaB pathway but lacking LTBR mediated induction of NFkappaB target genes revealed an inverse association of cellular TRAF3 levels with LTBR-specific defect in canonical NFkappaB activation ... In contrast, activation of NFkappaB by TNF did not depend on TRAF3 levels
Biyashev et al., J Biol Chem 2010 (MAP Kinase Signaling System) : PPARgamma , in turn is critical for NFkappaB activation, PEDF dependent apoptosis, and anti-angiogenesis
Shapiro et al., Inflammation 2010 (Acute Lung Injury...) : For instance, insulin inhibits NF-kappaB -- dependent synthesis of pro-inflammatory factors and attenuates production of ROS
Wang et al., Exp Eye Res 2010 : Treatment of ARPE-19 cells with CRP resulted in enhanced NF-kB nuclear translocation and dose dependent transient induction of IL-8 mRNA synthesis and protein secretion
Moon et al., Int Immunopharmacol 2010 (Arthritis, Experimental...) : Additionally, by altering nuclear factor (NF)-kappaB transcription activity in FLSs, curcumin inhibited PGE ( 2 ) production, COX-2 expression, and MMP secretion ... Additionally, by altering nuclear factor (NF)-kappaB transcription activity in FLSs, curcumin inhibited PGE ( 2 ) production, COX-2 expression, and MMP secretion ... Additionally, by altering nuclear factor (NF)-kappaB transcription activity in FLSs, curcumin inhibited PGE ( 2 ) production, COX-2 expression, and MMP secretion
Schneider et al., Oncogene 2010 : Experiments with knockout cells show that p65 and p53 are both required for enhanced NF-kappaB activity during S-phase checkpoint activation involving ataxia-telangiectasia mutated and checkpoint kinase-1 ... Experiments with knockout cells show that p65 and p53 are both required for enhanced NF-kappaB activity during S-phase checkpoint activation involving ataxia-telangiectasia mutated and checkpoint kinase-1 ... Hence, p53 is unexpectedly necessary for NF-kappaB mediated gene expression induced by atypical and classical stimuli
Moon et al., Arch Pharm Res 2010 : To elucidate the role of protein kinase C ( PKC ) in the upregulation of NF-kappaB by NMU and NEU , two known PKC inhibitors, staurosporine and H-7 were studied ... To elucidate the role of protein kinase C ( PKC ) in the upregulation of NF-kappaB by NMU and NEU, two known PKC inhibitors, staurosporine and H-7 were studied ... The results demonstrate the upregulatory effect of NMU and NEU on cellular NF-kappaB activity in human keratinocytes via the protein kinase C-mediated pathway ... The results demonstrate the upregulatory effect of NMU and NEU on cellular NF-kappaB activity in human keratinocytes via the protein kinase C-mediated pathway
Chao et al., J Agric Food Chem 2010 : By means of confocal microscope analysis we demonstrated that phosphorylation and membrane translocation of protein kinase C-alpha , as well as NF-kappaB activation , were inhibited by honokiol in LPS stimulated macrophages
He et al., Chin Med J (Engl) 2010 : However, DEX had little effect on LPS induced NF-kappaB activation, and partially impaired LPS induced I-kappaBalpha degradation
Wu et al., Environ Health Perspect 2010 : Phosphorylation and degradation of IkappaBalpha , an inhibitor of NF-kappaB , and phosphorylation of p65 were detected using immunoblotting
Gonsalves et al., J Immunol 2010 (Anoxia) : Hypoxia induced FLAP mRNA expression involved activation of NADPH-oxidase, PI-3 kinase, mitogen activated protein kinase, NF-kappaB , and hypoxia-inducible factor (HIF)-1alpha
Kim et al., J Immunol 2010 : We also characterized the BLT2 downstream signaling pathway and determined that BLT2 mediated IL-8 synthesis involves the upregulation of Nox1, a member of the NADPH oxidase family, Nox1 dependent reactive oxygen species generation and the subsequent activation of the redox-sensitive transcription factor NF-kappaB
Adli et al., PloS one 2010 : IKKalpha and IKKbeta each function to regulate NF-kappaB activation in the TNF-induced/canonical pathway ... IKKalpha and IKKbeta each function to regulate NF-kappaB activation in the TNF-induced/canonical pathway ... Analysis of NF-kappaB activation in response to TNF in MEFs reveals that IKKbeta is essential for efficient phosphorylation and subsequent degradation of IkappaB alpha, yet IKKalpha contributes to the NF-kappaB activation response in these cells as measured via DNA binding assays ... Analysis of NF-kappaB activation in response to TNF in MEFs reveals that IKKbeta is essential for efficient phosphorylation and subsequent degradation of IkappaB alpha, yet IKKalpha contributes to the NF-kappaB activation response in these cells as measured via DNA binding assays ... In HeLa cells, both IKKalpha and IKKbeta contribute to IkappaB alpha phosphorylation and NF-kappaB activation ... In HeLa cells, both IKKalpha and IKKbeta contribute to IkappaB alpha phosphorylation and NF-kappaB activation ... A kinase inactive mutant of IKKbeta, which has been used as evidence for the critical importance of IKKbeta in TNF induced signaling, blocks activation of NF-kappaB induced by IKKalpha , even in cells that are deficient in IKKbeta
Fritz et al., Mol Cancer Res 2010 (Breast Neoplasms...) : Ectopic expression of CNK1 elevates MT1-MMP promoter activity in a NF-kappaB dependent manner
Yan et al., Cancer Res 2010 (Carcinoma, Hepatocellular...) : The interaction between RARgamma and p85alpha resulted in activation of Akt and NF-kappaB , critical regulators of the growth and survival of cancer cells ... The interaction between RARgamma and p85alpha resulted in activation of Akt and NF-kappaB , critical regulators of the growth and survival of cancer cells
Dong et al., Exp Mol Med 2010 (Lung Neoplasms) : It has been shown that NQO1 is also essential for the TNF induced activation of NF-kappaB and that beta-lap suppresses the TNF induced NF-kappaB activation ... It has been shown that NQO1 is also essential for the TNF induced activation of NF-kappaB and that beta-lap suppresses the TNF induced NF-kappaB activation ... Irradiation with 4 Gy markedly increased the DNA binding activity of NF-kappaB in A549 cells, but not in the shNQO1 A549 cells, thus demonstrating that NQO1 plays a pivotal role in irradiation induced NF-kappaB activation ... It is concluded that beta-lap suppresses the radiation induced activation of NF-kappaB by interrupting the involvement of NQO1 in the activation of NF-kappaB , thereby inhibiting the transcription of survival signals ... The radiosensitization caused by beta-lap may, in part, be attributed to beta-lap induced suppression of NF-kappaB activation
Burgess et al., CNS Neurol Disord Drug Targets 2010 (Disease Models, Animal...) : The persistent activation of cRaf-1 can activate the proinflammatory factor NFkappaB and consequently, upregulate the expression of several of its downstream factors such as the amyloid precursor protein (APP), Cox-2 and iNOS
Aurora et al., Blood 2010 : Conversely, on cFLIP promoter, NF-kappaB increases histone deacetylase 1 (HDAC1) , decreases p300 and histone acetylation, and reduces the recruitment of NFAT, a transcription factor critical for cFLIP expression
Huang et al., Mediators Inflamm 2009 (MAP Kinase Signaling System) : Upregulation of Salmonella induced IL-6 production in Caco-2 cells by PJ-34, PARP-1 inhibitor : involvement of PI3K, p38 MAPK, ERK, JNK, and NF-kappaB
Tobar et al., Mol Cell Biochem 2010 : We found that TGF-beta1 activates NFkappaB , through Rac1-NOXs-ROS dependent mechanism ... Our results shows that TGF-beta1 stimulation of uPA and MMP-9 expression involve NOXs dependent ROS and NFkappaB , activation, demonstrated by using DPI, NOXs inhibitor, ROS scavenger N-acetylcysteine and SN50, an NFkb inhibitor ... Our results shows that TGF-beta1 stimulation of uPA and MMP-9 expression involve NOXs dependent ROS and NFkappaB , activation, demonstrated by using DPI, NOXs inhibitor, ROS scavenger N-acetylcysteine and SN50, an NFkb inhibitor ... Our results shows that TGF-beta1 stimulation of uPA and MMP-9 expression involve NOXs dependent ROS and NFkappaB , activation, demonstrated by using DPI, NOXs inhibitor, ROS scavenger N-acetylcysteine and SN50, an NFkb inhibitor ... Thus, ROS-NFkappaB acts as the crucial signal in TGF-beta1 induced uPA and MMP-9 expression thereby mediating the enhancement of cellular malignity by TGF-beta1 ... Thus, ROS-NFkappaB acts as the crucial signal in TGF-beta1 induced uPA and MMP-9 expression thereby mediating the enhancement of cellular malignity by TGF-beta1
Guleng et al., World J Gastroenterol 2010 (Acute Disease...) : Mindin expression is upregulated during intestinal inflammation and may induce NF-kappaB promoter activation in a TLR-9 mediated manner
Tanabe et al., Journal of neuroinflammation 2010 (Glioma) : TNF-alpha significantly induced phosphorylation of NFkappaB at Ser 536 and Ser 468, but not at Ser 529 or Ser 276 ... Wedelolactone, an inhibitor of IkappaB kinase, suppressed both TNF-alpha induced IkappaB phosphorylation and NFkappaB phosphorylation at Ser 536 and Ser 468 ... Wedelolactone, an inhibitor of IkappaB kinase , suppressed both TNF-alpha induced IkappaB phosphorylation and NFkappaB phosphorylation at Ser 536 and Ser 468 ... These results strongly suggest that TNF-alpha induces IL-6 synthesis through the JAK/STAT3 pathway in addition to p38 MAP kinase and SAPK/JNK in C6 glioma cells, and that phosphorylation of NFkappaB at Ser 536 and Ser 468, and NADPH oxidase are involved in TNF-alpha stimulated IL-6 synthesis ... These results strongly suggest that TNF-alpha induces IL-6 synthesis through the JAK/STAT3 pathway in addition to p38 MAP kinase and SAPK/JNK in C6 glioma cells, and that phosphorylation of NFkappaB at Ser 536 and Ser 468, and NADPH oxidase are involved in TNF-alpha stimulated IL-6 synthesis
Bardag-Gorce et al., Exp Mol Pathol 2010 (Disease Models, Animal...) : TRAF-6 activation leads to activation of NFkB and the mitogen activated protein kinase ( MAPK ) cascade
Ivanov et al., Cell Signal 2010 : The NF-kappaB dependent gene expression of IL8, IL6, PTGS2/COX2, TNF and IL33 in directly irradiated human skin fibroblasts produced the cytokines and prostaglandin E2 ( PGE2 ) with autocrine/paracrine functions, which further activated signaling pathways and induced NF-kappaB dependent gene expression in bystander cells ... The NF-kappaB dependent gene expression of IL8, IL6, PTGS2/COX2, TNF and IL33 in directly irradiated human skin fibroblasts produced the cytokines and prostaglandin E2 ( PGE2 ) with autocrine/paracrine functions, which further activated signaling pathways and induced NF-kappaB dependent gene expression in bystander cells ... The NF-kappaB dependent gene expression of IL8, IL6, PTGS2/COX2 , TNF and IL33 in directly irradiated human skin fibroblasts produced the cytokines and prostaglandin E2 ( PGE2 ) with autocrine/paracrine functions, which further activated signaling pathways and induced NF-kappaB dependent gene expression in bystander cells ... The NF-kappaB dependent gene expression of IL8, IL6 , PTGS2/COX2, TNF and IL33 in directly irradiated human skin fibroblasts produced the cytokines and prostaglandin E2 ( PGE2 ) with autocrine/paracrine functions, which further activated signaling pathways and induced NF-kappaB dependent gene expression in bystander cells ... The NF-kappaB dependent gene expression of IL8 , IL6, PTGS2/COX2, TNF and IL33 in directly irradiated human skin fibroblasts produced the cytokines and prostaglandin E2 ( PGE2 ) with autocrine/paracrine functions, which further activated signaling pathways and induced NF-kappaB dependent gene expression in bystander cells ... The NF-kappaB dependent gene expression of IL8, IL6, PTGS2/COX2 , TNF and IL33 in directly irradiated human skin fibroblasts produced the cytokines and prostaglandin E2 ( PGE2 ) with autocrine/paracrine functions, which further activated signaling pathways and induced NF-kappaB dependent gene expression in bystander cells ... The NF-kappaB dependent gene expression of IL8, IL6, PTGS2/COX2 , TNF and IL33 in directly irradiated human skin fibroblasts produced the cytokines and prostaglandin E2 ( PGE2 ) with autocrine/paracrine functions, which further activated signaling pathways and induced NF-kappaB dependent gene expression in bystander cells
Martinez-Orozco et al., Eur J Cell Biol 2010 (Neoplasm Invasiveness) : We demonstrate here that AA promotes an increase in vimentin and N-cadherin expression, MMP-9 secretion, a decrease in E-cadherin junctional levels, and the activation of FAK, Src and NF-kappaB in MCF10A cells ... We demonstrate here that AA promotes an increase in vimentin and N-cadherin expression, MMP-9 secretion, a decrease in E-cadherin junctional levels, and the activation of FAK, Src and NF-kappaB in MCF10A cells
Liu et al., Cancer Lett 2010 (Prostatic Neoplasms) : In this study, we investigated the roles of OPN in human prostate cancer cells and provided clues about the possible functions of IkappaB kinase (IKK) in NF-kappaB mediated OPN induced activations of MMP-2 and MMP-9 ... In this study, we investigated the roles of OPN in human prostate cancer cells and provided clues about the possible functions of IkappaB kinase (IKK) in NF-kappaB mediated OPN induced activations of MMP-2 and MMP-9
Yasuda et al., Tohoku J Exp Med 2010 (Arthritis, Rheumatoid) : Anti-CD44 antibody reversed the inhibitory effects of HA on the LPS mediated increase in PGE ( 2 ) production, COX-2 induction, and activation of NF-kappaB ... Anti-CD44 antibody reversed the inhibitory effects of HA on the LPS mediated increase in PGE ( 2 ) production, COX-2 induction, and activation of NF-kappaB ... Anti-CD44 antibody reversed the inhibitory effects of HA on the LPS mediated increase in PGE ( 2 ) production, COX-2 induction , and activation of NF-kappaB
Paris et al., Journal of neuroinflammation 2010 (Alzheimer Disease...) : In vitro, celastrol dose dependently prevented NFkappaB activation and inhibited BACE-1 expression
Neumann et al., Molecular systems biology 2010 : We established an integrated kinetic mathematical model for CD95 mediated apoptotic and NF-kappaB signaling
Gomard et al., PloS one 2010 (Inflammation) : An NF-kappaB dependent role for JunB in the induction of proinflammatory cytokines in LPS activated bone marrow derived dendritic cells
Cooper et al., Cell Stress Chaperones 2010 : Similarly, FRT exposure selectively reduced LPS induced NFkappaB-p65 recruitment to the kappaB enhancer site at -510 without affecting the other three kappaB enhancer sites present in the TNF-alpha 5'-flanking sequence
Kusunoki et al., Arthritis Rheum 2010 (Arthritis, Rheumatoid) : Enhancement of NF-kappaB activation by adiponectin as well as by interleukin-1beta was observed in RASFs
Chassaing et al., Br J Dermatol 2010 (Ectodermal Dysplasia...) : EDARADD interacts with the TAB2/TRAF6/TAK1 complex, which is necessary for NF-kappaB activation by EDAR
Lee et al., EMBO J 2010 : Interestingly, loss of CYLD causes constitutive NF-kappaB activation in developing NKT cells, which contributes to their defective IL-7 response and attenuated ICOS expression
Fritzenwanger et al., Mediators Inflamm 2009 (Heart Failure) : CT-1 caused a dose dependent nuclear factor (NF) kappaB translocation
Peng et al., Pharm Res 2010 (Prostatic Neoplasms) : WCTE treatment to PC-3 cells resulted in ( 1 ) induction of WAF1/p21 and KIP1/p27, ( 2 ) decrease in cyclins D1, D2 and E, ( 3 ) decrease in cyclin dependent kinase (cdk) 2, 4 and 6, ( 4 ) induction of Bax and down-regulation of Bcl-2, ( 5 ) decrease in procaspase-3, -8, ( 6 ) inhibition of nuclear translocation and phosphorylation of NF-kappaB and activation of IKKalpha , and ( 7 ) inhibition of phosphorylation and degradation of IkappaBalpha
Tang et al., Am J Med Sci 2010 (Cell Transformation, Neoplastic...) : LPS increased the phosphorylation of ERK1/2 and P38 and activated NF-kappaB
Boukerche et al., Oncogene 2010 (Melanoma) : MDA-9/syntenin also promotes melanoma metastasis by activating c-Src, but how c-Src regulates NF-kappaB activation is unclear ... Inhibition of c-Src by PP2 treatment, by blocking c-Src or mda-9/syntenin expression with small interfering RNA, or in c-Src ( -/- ) knockout cell lines, reduces NF-kappaB activation following overexpression of mda-9/syntenin or c-Src
Yuan et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2010 : [ Effects of the inhibitor of NF-kappaB and IFN-gamma on BAFF-R gene promoter activity ]
Wei et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2010 (Diabetes, Gestational...) : The inhibition of NF-kappaB activity can increase the expression of GLUT-4 in uterus of GDM rats, indicating that the occurrence of GDM in rats with uteru insulin resistance may be related with the intervention of NF-kappaB activity on the down regulation of the expression of GLUT-4
Jin et al., J Biol Chem 2010 : In this study, we found that MAML1 regulates NF-kappaB signaling via two mechanisms ... Maml1-deficient mouse embryonic fibroblasts showed impaired tumor necrosis factor-alpha (TNFalpha) induced NF-kappaB responses
Nishida et al., J Biol Chem 2010 : PTX increased the expression of IL-1beta and AT1R through NF-kappaB , and a small GTP binding protein, Rac, mediated PTX induced NF-kappaB activation through NADPH oxidase dependent production of reactive oxygen species ... PTX increased the expression of IL-1beta and AT1R through NF-kappaB, and a small GTP binding protein , Rac, mediated PTX induced NF-kappaB activation through NADPH oxidase dependent production of reactive oxygen species
Yoon et al., Infect Immun 2010 : Inhibition of p38 mitogen activated protein kinase ( MAPK ) using SB203580 and small interfering RNA ( siRNA ) transfection resulted in a significant reduction in BFT induced I kappaB kinase (IKK)/NF-kappaB activation and hBD-2 expression
Polyak et al., Proc Natl Acad Sci U S A 2010 (Hepatitis C) : Activities tested included inhibition of : HCV cell culture infection, NS5B polymerase activity, TNF-alpha induced NF-kappaB transcription, virus induced oxidative stress, and T-cell proliferation
Kim et al., J Cell Physiol 2010 : We conclude that NECA stimulated Cx43 phosphorylation mediated by PI3K/Akt, PKC, MAPKs, and NF-kappaB , which subsequently stimulated cell migration and proliferation through Src, integrin beta1, FAK, and paxillin signal pathways
Ding et al., Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi 2009 : To investigate on the effect of Plasmodium yoelii ( BY265 strain ) circumsporozoite protein (CSP) on the activation of nuclear transcription factor KB (NF-KB) in hepatoma cells ( HepG2 ) stimulated by TNF-alpha ... Dual-luciferase assay and EMSA were performed to detect the nuclear translocation and activation of NF-kappaB, to observe if pFLAG-CMV8-CSP suppressed the activation of NF-KB in HepG2 stimulated by TNF-alpha ... Plasmodium yoelii CSP localizes in the cytoplasm of HepG2 and inhibits the activation and nuclear translocation of NF-kappaB in HepG2 stimulated by TNF-alpha
Zocco et al., Mol Immunol 2010 (Calcium Signaling) : Histamine modulation of peripheral CRH receptor type 1alpha expression is dependent on Ca ( 2+ ) signalling and NF-kappaB/p65 transcriptional activity ... Functional consequences of enhanced CREB, NF-kappaB and NR4A2 activity confirm that NF-kappaB/p65 selectively controls CRH-R1 promoter activity
Lee et al., J Biol Chem 2010 : T cell receptor stimulated NF-kappaB activation requires CARMA1 and is negatively regulated by the deubiquitinase CYLD ... T cell receptor stimulated NF-kappaB activation requires CARMA1 and is negatively regulated by the deubiquitinase CYLD ... We show that CYLD deficiency causes constitutive NF-kappaB activation in thymocytes, which is associated with enhanced frequency of Treg cells ... The NF-kappaB activation in CYLD-deficient thymocytes is independent of CARMA1 , because the NF-kappaB activation was also detected in CYLD/CARMA1 double knock-out thymocytes ... Interestingly, although loss of CYLD causes NF-kappaB activation in the CARMA1-deficient thymocytes, the CYLD deficiency fails to rescue the defect of CARMA1 knock-out mice in Treg development
Liu et al., J Biol Chem 2010 : Furthermore, AMPK activation significantly enhanced IkappaBalpha kinase activation, NF-kappaB nuclear translocation, and DNA binding activity of NF-kappaB ... Furthermore, AMPK activation significantly enhanced IkappaBalpha kinase activation , NF-kappaB nuclear translocation, and DNA binding activity of NF-kappaB ... Overall, our results suggest that AMPKalpha1, but not AMPKalpha2 activation, promotes cell survival by increasing NF-kappaB mediated expression of anti-apoptotic proteins ( Bcl-2 and Survivin ) and intracellular ATP contents
Chan et al., J Pathol 2010 (Lymphoma, Extranodal NK-T-Cell) : Interleukin-2 induces NF-kappaB activation through BCL10 and affects its subcellular localization in natural killer lymphoma cells ... Interleukin-2 induces NF-kappaB activation through BCL10 and affects its subcellular localization in natural killer lymphoma cells
Saito et al., American journal of physiology. Renal physiology 2010 : TNF-alpha caused activation of NF-kappaB , MAP kinases, and PI3K-Akt in podocytes, whereas blockade of these molecules did not affect inhibition of RAR by TNF-alpha
Cherfils-Vicini et al., J Clin Invest 2010 (Lung Neoplasms) : Stimulation with TLR7 or TLR8 agonists led to activated NF-kappaB , upregulated expression of the antiapoptotic protein Bcl-2, increased tumor cell survival, and chemoresistance ... Stimulation with TLR7 or TLR8 agonists led to activated NF-kappaB , upregulated expression of the antiapoptotic protein Bcl-2, increased tumor cell survival, and chemoresistance
Jeon et al., Cell Mol Life Sci 2010 : Annexin A4 interacts with the NF-kappaB p50 subunit and modulates NF-kappaB transcriptional activity in a Ca2+ dependent manner ... Following treatment with TNF-alpha or PMA, ANXA4 also suppressed NF-kappaB transcriptional activity, which was upregulated significantly early after etoposide treatment
Oh et al., Neurosci Lett 2010 (Inflammation) : We showed that oleamide blocked LPS induced NF-kappaB activation and phosphorylation of inhibitor kappaB kinase (IKK) ... Finally, we showed that a specific antagonist of the CB2 receptor, AM630, blocked the inhibitory effects of oleamide on LPS induced production of proinflammatory mediators and activation of NF-kappaB
Heo et al., Vascul Pharmacol 2010 (Atherosclerosis) : Furthermore, the NADPH oxidase assembly inhibitor, AEBSF, and the blocker of NADPH oxidase, p47(phox) small interference RNA ( siRNA ), also efficiently blocked LIGHT induced migration, CCR1, CCR2, ICAM-1, and HVEM expression, and p38 MAPK and NF-kB activation
Halle et al., J Am Coll Cardiol 2010 (Cardiovascular Diseases...) : We also suggest that HOXA9 might be involved in the regulation of NF-kappaB activation
Taylor et al., AIDS 2010 (HIV Infections) : We have previously shown that Casp8p41 induced NFkappaB activation enhances HIV LTR transcription and consequently increases HIV replication ... We have previously shown that Casp8p41 induced NFkappaB activation enhances HIV LTR transcription and consequently increases HIV replication ... Herein, we questioned whether Casp8p41 induced NFkappaB activation impacts the cytokine profile of cells expressing Casp8p41 ... Herein, we questioned whether Casp8p41 induced NFkappaB activation impacts the cytokine profile of cells expressing Casp8p41
Segal et al., PloS one 2010 (Granulomatous Disease, Chronic...) : When challenged with either intratracheal zymosan or LPS, NADPH oxidase-deficient p47 ( phox-/- ) mice and gp91(phox)-deficient mice developed exaggerated and progressive lung inflammation, augmented NF-kappaB activation , and elevated downstream pro-inflammatory cytokines ( TNF-alpha , IL-17, and G-CSF ) compared to wildtype mice ... When challenged with either intratracheal zymosan or LPS, NADPH oxidase-deficient p47 ( phox-/- ) mice and gp91(phox)-deficient mice developed exaggerated and progressive lung inflammation, augmented NF-kappaB activation , and elevated downstream pro-inflammatory cytokines ( TNF-alpha, IL-17, and G-CSF ) compared to wildtype mice ... When challenged with either intratracheal zymosan or LPS, NADPH oxidase-deficient p47 ( phox-/- ) mice and gp91(phox)-deficient mice developed exaggerated and progressive lung inflammation, augmented NF-kappaB activation , and elevated downstream pro-inflammatory cytokines ( TNF-alpha, IL-17 , and G-CSF ) compared to wildtype mice
Mizutani et al., Pulm Pharmacol Ther 2010 : The level of IkappaBalpha protein was increased from 30 min, suggesting an increase in the NF-kappaB mediated transcription of IkappaBalpha
Huo et al., Gastroenterology 2010 (Barrett Esophagus...) : Bay 11-705, Ad5 inhibitor kappaB(IkappaB)alpha-SR, and site directed mutagenesis were used to explore effects of nuclear factor-kappaB (NF-kappaB) inhibition on CDX2 promoter activity ; DNA binding of the NF-kappaB subunits p50 and p65 was assessed by chromatin immune-precipitation
Jain et al., Mol Nutr Food Res 2010 (Diabetes Mellitus, Type 2...) : While CDN and CP had no effect, activation of NFkappaB , Akt and glucose transporter-2 levels were decreased, insulin receptor substrate 1 (IRS-1) activation increased in livers of CDNC-rats ... While CDN and CP had no effect, activation of NFkappaB , Akt and glucose transporter-2 levels were decreased, insulin receptor substrate 1 (IRS-1) activation increased in livers of CDNC-rats ... CDNC is a potent hypoglycemic compound with anti-inflammatory activity apparently mediated by elevated blood vitamin C and adiponectin and inhibition of NFkappaB , Akt, and Glut-2 and increased IRS-1 activation in livers of type 2 diabetic rats
Rushworth et al., Cancer Res 2010 (Acute Disease...) : We show that inhibition of NF-kappaB increased HO-1 expression in primary AML cells compared with that of nonmalignant cells
Bangaru et al., Anticancer Res 2010 (Cerebellar Neoplasms...) : The expression of both bcl-2 and MMP-9 is regulated by the transcription factor NF-kappaB
Faugaret et al., Mol Immunol 2010 (MAP Kinase Signaling System) : We found that TNFalpha preferentially used p38MAPK to induce phenotype maturation in DC, whereas LPS preferentially activated NF-kappaB
Cardile et al., Clin Exp Pharmacol Physiol 2010 (Inflammation) : 1. The novel nuclear factor (NF)-kappaB inhibitor dehydroxymethylepoxyquinomicin ( DHMEQ ) is a derivative of the antibiotic epoxyquinomicin C from Amycolatopsis sp. that has been found to inhibit tumour necrosis factor (TNF)-alpha induced activation of NF-kappaB by suppressing nuclear translocation of NF-kappaB
Luckprom et al., J Periodontal Res 2010 (Bone Resorption) : A cAMP dependent protein kinase inhibitor, H89, and a nuclear factor kappaB (NF kappaB) inhibitor, pyrrolidine dithiocarbamate, inhibited RANKL expression, prostaglandin E ( 2 ) production and NF kappaB translocation
Ando et al., FEBS J 2010 : Human lactoferrin activates NF-kappaB through the Toll-like receptor 4 pathway while it interferes with the lipopolysaccharide stimulated TLR4 signaling ... Endotoxin depleted hLF induces NF-kappaB activation at physiologically relevant concentrations in the human monocytic leukemia cell line, THP-1, and in mouse embryonic fibroblasts ( MEFs ) ... In MEFs, in which both tumor necrosis factor receptor associated factor 2 ( TRAF2 ) and TRAF5 are deficient, hLF causes NF-kappaB activation at a level comparable to that seen in wild-type MEFs, whereas TRAF6-deficient MEFs show significantly impaired NF-kappaB activation in response to hLF ... When we examined the hLF dependent NF-kappaB activation in MyD88-deficient MEFs, delayed, but remarkable, NF-kappaB activation occurred as a result of the treatment of cells with hLF, indicating that both MyD88 dependent and MyD88 independent pathways are involved
Hoyle et al., Am J Physiol Lung Cell Mol Physiol 2010 (Pneumonia) : Triptolide inhibited IL-8 production induced by substance P with an IC ( 50 ) of 2.3 x 10 ( -8 ) M and inhibited NF-kappaB activation in response to an agonist of the protease activated receptor 2 with an IC ( 50 ) of 1.4 x 10 ( -8 ) M. Anti-inflammatory effects of triptolide were assessed in vivo using a chlorine gas lung injury model in mice
Ma et al., J Immunol 2010 : The NF-kappaB pathway inhibitor BAY-11, JNK inhibitor JNKi II, and p38 inhibitor SB203580 suppressed the synergistic effect of IL-6 and IL-17 on IL-6 expression
Murphy et al., J Immunol 2010 : Furthermore, we show that activation of macrophages with LPS decreases the expression of kappaB-Ras2 , an inhibitor of NF-kappaB signaling ... We propose a novel mechanism for estradiol inhibition of LPS induced NF-kappaB signaling in which kappaB-Ras2 expression is induced by estradiol via regulation of let-7a and miR-125b ... These findings are significant in that they are the first to demonstrate that estradiol represses NF-kappaB activation through the induction of kappaB-Ras2 , a key inhibitor of NF-kappaB signaling
Lee et al., Eur J Pharmacol 2010 (MAP Kinase Signaling System) : Moreover, eupatolide significantly suppressed the LPS induced expression of nuclear factor-kappa B (NF-kappaB) and activator protein-1 (AP-1) reporter genes
Young et al., Bone 2010 : Activation of NF-kappaB by fluid shear stress, but not TNF-alpha , requires focal adhesion kinase in osteoblasts ... We examined the role of a key component of focal adhesions and of mechanotransduction, focal adhesion kinase ( FAK ) in regulation of FSS- and tumor necrosis factor-alpha (TNF-alpha) induced activation of nuclear factor-kappa B (NF-kappaB) signaling in osteoblasts ... Interestingly, FAK was not required for TNF-alpha induced NF-kappaB activation in osteoblasts
Kim et al., Mol Cancer Ther 2010 (Carcinoma, Hepatocellular...) : beta-Ionone enhances TRAIL induced apoptosis in hepatocellular carcinoma cells through Sp1 dependent upregulation of DR5 and downregulation of NF-kappaB activity ... In addition, ION significantly increased hepatocellular carcinoma cell sensitivity to TRAIL by abrogating TRAIL induced NF-kappaB activation and decreasing the expression of antiapoptotic proteins such as XIAP and IAP-1/2
Deng et al., Cancer prevention research (Philadelphia, Pa.) 2010 (Adenocarcinoma...) : Thus, Gprc5a loss enhances NF-kappaB activation in lung epithelial cells, leading to increased autocrine and paracrine interactions, cell autonomy, and enhanced inflammation, which may synergize in the creation of a tumor promoting microenvironment
Damm et al., J Invest Dermatol 2010 (Lymphatic Metastasis...) : HGF dependent CD44v6 regulation in melanocytes is NF-kappaB dependent because BAY 11-7082, an inhibitor of NF-kappaB activation, but not interference with the mitogen activated protein kinase or phosphatidylinositol 3-kinase cascade, antagonized HGF induced CD44v6 expression ... NF-kappaB mediated transcriptional regulation of CD44v6 involves the transcription factors Egr-1 and CCAAT enhancer binding protein-beta ( C/EBP-beta )
Granado-Serrano et al., Nutr Cancer 2010 (Carcinoma, Hepatocellular...) : These data suggest that NF-kappa B and AP-1 play a main role in the tight regulation of survival/proliferation pathways exerted by quercetin and that the sustained JNK/AP-1 activation and inhibition of NF-kappa B provoked by the flavonoid induced HepG2 death
Souslova et al., PloS one 2010 (Atherosclerosis) : Allele-specific regulation of matrix metalloproteinase-3 gene by transcription factor NFkappaB
Kim et al., Food Chem Toxicol 2010 (Inflammation) : Further studies revealed that EC extracts suppress the production of tumor necrosis factor-alpha (TNF-alpha) and activation of nuclear factor-kappa B (NF-kappaB)
Li et al., Molecular cancer 2010 : In this study, we demonstrate that MA significantly enhanced TNFalpha induced inhibition of pancreatic cancer cell proliferation, invasion, and potentiated TNFalpha induced cell apoptosis by suppressing TNFalpha induced NF-kappaB activation in a dose- and time dependent manner ... Our data demonstrate that MA can potentiate the anti-tumor activities of TNFalpha and inhibit pancreatic tumor growth and invasion by activating caspase dependent apoptotic pathway and by suppressing NF-kappaB activation and its downstream gene expression
Baritaki et al., Crit Rev Immunol 2010 (Neoplasms...) : Because RKIP negatively regulates NF-kappaB , we propose that the resistant cell phenotype could be maintained through Snail mediated RKIP suppression which supports the constitutive NF-kappaB activation
Arntz et al., Arthritis Res Ther 2010 (Arthritis, Experimental...) : In vitro, HpTNFR1 reduced the TNFR1 mRNA expression by three-fold resulting in a 70 % reduction of TNFalpha induced NF-kappaB activation
Wang et al., FEBS Lett 2010 : TSA-treatment inhibited nuclear translocation of NF-kappaB following IgE mediated, but not LPS induced activation in MCs
Nishida et al., Int J Mol Med 2010 (Inflammation) : Monoacetylcurcumin also inhibited the LPS induced NF-kappaB activation in the liver
Dominiak et al., Pharm Res 2010 (Neoplasms) : The mean NF-kappaB DNA binding activity was increased upon TNF-alpha treatment in pre-intervention samples ; however, TNF-alpha was unable to induce NF-kappaB in post-intervention samples, suggesting that the mixture of four important natural agents could be useful to protect humans against oxidative stress
Li et al., Toxicological sciences : an official journal of the Society of Toxicology 2010 (Cell Transformation, Neoplastic) : The repressive effect of NF-kappaB on p53 by mot-2 is involved in human keratinocyte transformation induced by low levels of arsenite ... Together, the results suggest that the repressive effect of NF-kappaB on p53 by mot-2 leads to genomic instability, which is involved in arsenite induced malignant transformation of human keratinocytes
Jurkin et al., J Immunol 2010 (Bacterial Infections) : Inversely, silencing of miR-146a in LCs enhanced TLR2 dependent NF-kappaB signaling ... Inversely, silencing of miR-146a in LCs enhanced TLR2 dependent NF-kappaB signaling
Pistolic et al., J Innate Immun 2009 : Using specific inhibitors it was shown that NF-kappaB signaling led to the LL-37 stimulated production of IL-6
Robinson et al., J Innate Immun 2010 : Additionally, IL-27 blocked NF-kappaB activation in response to IL-18 ... Additionally, IL-27 blocked NF-kappaB activation in response to IL-18
Fotio et al., Int J Immunopathol Pharmacol 2010 (Liver Failure) : In addition, S. birrea inhibited the nuclear factor kB (NF-kB) activation by both LPS and BCG
O'Gorman et al., Am J Physiol Gastrointest Liver Physiol 2010 : Regulation of NF-kappaB responses by epigenetic suppression of IkappaBalpha expression in HCT116 intestinal epithelial cells ... Tumor necrosis factor-alpha induced NF-kappaB activation was blocked and IkappaBalpha expression was higher in HCT116 cells lacking DNMT than in parental cells
Ehrenschwender et al., Cell Death Differ 2010 (Neoplasm Invasiveness) : In mutant PIK3CA expressing cells, tumor necrosis factor related apoptosis inducing ligand ( TRAIL ) and CD95L stimulated nuclear factor kappaB (NFkappaB) activation, invasion, and transition to an amoeboid-like morphology ... NFkappaB activation and adoption of amoeboid shape were inhibited by caspase-8 knockdown or FLIP-S expression, but only the cell morphology alterations required caspase-8 activity
Zhang et al., Diabetes Obes Metab 2010 (Diabetes Mellitus, Experimental...) : The effects of TSN on Akt and NF-kappaB phosphorylation and the expression of tumour necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) in cardiac tissues were examined ... Western blot analysis showed that TSN treatment enhanced Akt phosphorylation and inhibited NF-kappaB phosphorylation in cardiac tissues
Ide et al., Microvasc Res 2010 (Diabetic Nephropathies) : PEDF or neutralizing antibody raised against RAGE ( receptor for AGE ) was found to inhibit the AGE induced oxidative stress generation and subsequent NF-kappaB activation in mesangial cells ... PEDF or neutralizing antibody raised against RAGE ( receptor for AGE ) was found to inhibit the AGE induced oxidative stress generation and subsequent NF-kappaB activation in mesangial cells
Cao et al., Int Immunopharmacol 2010 (Disease Models, Animal...) : We further observed that MA prevented the LPS induced nuclear factor-kappa B (NF-kappaB) translocation from the cytoplasm into the nucleus, and inhibited the LPS induced phosphorylation of extracellular signal regulated kinase 1/2 ( ERK1/2 ) and p38 ... We further observed that MA prevented the LPS induced nuclear factor-kappa B (NF-kappaB) translocation from the cytoplasm into the nucleus, and inhibited the LPS induced phosphorylation of extracellular signal regulated kinase 1/2 ( ERK1/2 ) and p38 ... We further observed that MA prevented the LPS induced nuclear factor-kappa B (NF-kappaB) translocation from the cytoplasm into the nucleus, and inhibited the LPS induced phosphorylation of extracellular signal regulated kinase 1/2 ( ERK1/2 ) and p38
Zhang et al., Am J Physiol Heart Circ Physiol 2010 (Disease Models, Animal...) : Luciferase assay demonstrated an activation of NF-kappaB by HDAC inhibition
Premkumar et al., BMC chemical biology 2010 : Also, the suppressive effect of triptolide on TLR induced NFkappaB activation was observed when either MyD88 or TRIF was knocked out, confirming that both MyD88 and TRIF mediated NFkappaB activation may be inhibited by triptolide
Shifera et al., Immunol Lett 2010 : Also, 1alpha,25 ( OH ) ( 2 ) D ( 3 ) did not suppress the activation of NF-kappaB by TNFalpha or PHA
Lin et al., Peptides 2010 : Pretreatment with the SALF significantly downregulated the expression of nuclear factor (NF)-kappaB in the presence of LPS
Ho et al., Free Radic Biol Med 2010 : Transcriptional regulation of UCP4 by NF-kappaB and its role in mediating protection against MPP+ toxicity ... NF-kappaB inhibition significantly suppressed the MPP ( + ) -induced increase in UCP4 expression ... We present the first direct evidence that UCP4 is regulated by NF-kappaB , mediated via a functional NF-kappaB site in its promoter region, and that UCP4 has a significant role in NF-kappaB prosurvival signaling, mediating its protection against MPP ( + ) toxicity
Liu et al., Cancer Cell 2010 (Leukemia, Myeloid) : Our results provide evidence that the mechanisms of Sp1/NFkappaB/HDAC/miR-29b dependent KIT overexpression contribute to leukemia growth and can be successfully targeted by pharmacological disruption of the Sp1/NFkappaB/HDAC complex or synthetic miR-29b treatment in KIT-driven AML
Janbandhu et al., J Biol Chem 2010 : To study the involvement of NF-kappaB in G ( 1 ) /S phase regulation, the levels of selected transcriptional regulators were monitored following overexpression of NF-kappaB or its physiological induction by tumor necrosis factor-alpha
Peng et al., FASEB J 2010 : RSK2 mediates NF-{kappa}B activity through the phosphorylation of IkappaBalpha in the TNF-R1 pathway
Azcutia et al., PloS one 2010 (Hyperglycemia...) : Both ERK 1/2 and NF-kappaB were necessary for VCAM-1 expression, but not for ICAM-1 expression ... Both ERK 1/2 and NF-kappaB were necessary for VCAM-1 expression, but not for ICAM-1 expression
Nadiminty et al., Cancer Res 2010 (Disease Progression...) : Aberrant activation of the androgen receptor by NF-kappaB2/p52 in prostate cancer cells ... Comparison of the relative effects of p52 and p65 (RelA) showed that p52, but not p65 , could activate the AR. Collectively, these findings, together with previous reports that the levels of NF-kappaB2/p52 are elevated in prostate cancer cells and that active NF-kappaB2/p52 promotes prostate cancer cell growth in vitro and in vivo, suggest that NF-kappaB2/p52 may play a critical role in the progression of castration-resistant prostate cancer ... Comparison of the relative effects of p52 and p65 (RelA) showed that p52 , but not p65, could activate the AR. Collectively, these findings, together with previous reports that the levels of NF-kappaB2/p52 are elevated in prostate cancer cells and that active NF-kappaB2/p52 promotes prostate cancer cell growth in vitro and in vivo, suggest that NF-kappaB2/p52 may play a critical role in the progression of castration-resistant prostate cancer
Seo et al., J Physiol Pharmacol 2009 : Nitric oxide induced IL-8 expression is mediated by NF-kappaB and AP-1 in gastric epithelial AGS cells
Mogi et al., J Immunoassay Immunochem 2010 : Down-regulation of NF-kappaB led to up-regulation of NGF production in mouse osteoblasts ... These findings demonstrate that the suitable suppression of NF-kappaB nuclear translocation induced NGF production in non-neuronal osteoblastic cells
Imamura et al., J Immunol 2010 (Shock, Septic) : Interestingly, the NOD of human PYNOD was sufficient to inhibit caspase-1 mediated IL-1beta secretion, whereas its pyrin domain was sufficient to inhibit ASC mediated NF-kappaB activation and apoptosis and to reduce ASC 's ability to promote caspase-1 mediated IL-1beta production
Peña et al., J Mol Med (Berl) 2010 (Sepsis) : JAK2 inhibitor prevents the activation of the canonical p65RelA/p50NF-kappaB1 pathway but not the other NF-kappaB proteins
Watson et al., Brain Behav Immun 2010 (Encephalitis) : The evidence suggests that these changes are a likely consequence of increased hippocampal expression of CD14 and TLR4 , and NFkappaB activation in SIGIRR ( -/- ) mice ... The evidence suggests that these changes are a likely consequence of increased hippocampal expression of CD14 and TLR4, and NFkappaB activation in SIGIRR ( -/- ) mice
Munitz et al., Gastroenterology 2010 (Colitis...) : In vitro analysis showed increased production of proinflammatory cytokines ( interleukin-6, interleukin-1beta , and tumor necrosis factor alpha ) and activation of MAPK and NF-kappaB in Pirb-/- macrophages following bacterial activation ... In vitro analysis showed increased production of proinflammatory cytokines ( interleukin-6, interleukin-1beta , and tumor necrosis factor alpha ) and activation of MAPK and NF-kappaB in Pirb-/- macrophages following bacterial activation ... In vitro analysis showed increased production of proinflammatory cytokines ( interleukin-6 , interleukin-1beta, and tumor necrosis factor alpha ) and activation of MAPK and NF-kappaB in Pirb-/- macrophages following bacterial activation
Zheng et al., Eur J Pharmacol 2010 : NF-kappaB signaling mediates vascular smooth muscle endothelin type B receptor expression in resistance arteries
Gong et al., Pharmacol Biochem Behav 2010 (Alzheimer Disease...) : Meanwhile, LPS injection decreased H ( 2 ) S level but increased pro-inflammatory mediators ( i.e., TNF-alpha, TNFR1, degradation of IkappaB-alpha and thereafter activation of NF-kappaB ) in hippocampus
Iwakiri et al., Adv Cancer Res 2010 (Burkitt Lymphoma...) : Furthermore, EBERs induce IL-10 through IRF3 but not NF-kappaB activation in BL cells, suggesting that modulation of innate immune signaling by EBERs contribute to EBV mediated oncogenesis
Liu et al., J Immunol 2010 : Furthermore, overexpression of ST2 dose-dependently attenuated BLP induced NF-kappaB activation, suggesting a negative regulatory role of ST2 in TLR2 signaling ... Furthermore, overexpression of ST2 dose-dependently attenuated BLP induced NF-kappaB activation, suggesting a negative regulatory role of ST2 in TLR2 signaling
Lu et al., J Neurochem 2010 (Ischemia...) : The U0126 neuroprotection appears related to NF-kappaB regulated transcriptional control of c-IAP2 ... MEK/ERK inhibition also induced I-kappaB phosphorylation and enhanced nuclear factor (NF)-kappaB/DNA binding activity, leading to expression of cellular inhibitors of apoptosis protein 2 ( c-IAP2 ), a known nuclear factor-kappaB (NF-kappaB) regulated endogenous anti-apoptotic molecule
Kang et al., Experimental biology and medicine (Maywood, N.J.) 2010 : These results suggest that the differential regulation of STE on IFN-gamma, IL-4 and IL-2 may be due to its suppression of NF-kappaB , concomitant with its enhancement of NFATc2
Giraud et al., Proc Natl Acad Sci U S A 2010 (Encephalomyelitis, Autoimmune, Experimental) : Estradiol inhibits ongoing autoimmune neuroinflammation and NFkappaB dependent CCL2 expression in reactive astrocytes ... Chromatin immunoprecipitation assays further indicate that estrogen suppresses TNFalpha induced NFkappaB recruitment to the CCL2 enhancer
Jimenez et al., J Immunol 2010 (Leishmaniasis, Cutaneous) : We postulated that CCR2-driven activation of the transcription factor NF-kappaB plays a critical role in dendritic cell ( DC ) maturation ( e.g., migration, costimulation, and IL-12p70 production ), necessary for the generation of protective immune responses against the intracellular pathogen Leishmania major ... Supporting this notion, we found that CCR2, its ligand CCL2, and NF-kappaB were required for CCL19 production and adequate Langerhans cell ( LC ) migration both ex vivo and in vivo ... Collectively, our data point to an important role for CCR2-driven activation of NF-kappaB in the regulation of DC/LC maturation processes that regulate protective immunity against intracellular pathogens
Zidi et al., J Physiol Biochem 2010 : Soluble HLA-G induces NF-kappaB activation in natural killer cells ... To evaluate intracellular signaling in NK cells upon HLA-G soluble forms stimulation, we investigate the role of soluble HLA-G ( HLA-G5- and HLA-G1 shedding form ) stimulation on classical nuclear factor (NF)-kappaB pathway activation ... We reported that these two forms of soluble HLA-G could activate NF-kappaB in NK cells
Tare et al., Exp Cell Res 2010 (Leukemia, Myelogenous, Chronic, BCR-ABL Positive...) : The interaction of IL-33 and ST2L activated NF-kappaB , JNK, and p38 MAPK, but not ERK1/2 signaling pathways ... The interaction of IL-33 and ST2L activated NF-kappaB , JNK, and p38 MAPK, but not ERK1/2 signaling pathways
Bassères et al., Cancer Res 2010 (Cell Transformation, Neoplastic...) : Using lung cell lines expressing oncogenic K-Ras, we show that NF-kappaB is activated in these cells in a K-Ras dependent manner and that NF-kappaB activation by K-Ras requires inhibitor of kappaB kinase beta ( IKKbeta ) kinase activity
Kang et al., Diabetes Res Clin Pract 2010 (Diabetes Mellitus, Type 2...) : These findings suggest that visfatin synthesis is activated from adipose tissue in a diabetic environment, induces NF-kappaB activation and leads to activation of pro-inflammatory cytokines and systemic inflammation
Li et al., American journal of physiology. Renal physiology 2010 : By using the luciferase-promoter deletion assay, site directed mutagenesis, and transactivation assay, we identified a glycated albumin-responsive region in the USF2 gene promoter ( -837 to -430, relative to the transcription start site ) and demonstrated that glycated albumin induced USF2 expression was mediated through NF-kappaB dependent transactivation of the USF2 promoter ... Furthermore, glycated albumin increased nuclear NF-kappaB subunit-p65 protein levels ... Taken together, these data suggest that glycated albumin upregulated USF2 gene transcription in MCs through NF-kappaB dependent transactivation of the USF2 promoter
Yoboua et al., J Virol 2010 : Respiratory syncytial virus mediated NF-kappa B p65 phosphorylation at serine 536 is dependent on RIG-I, TRAF6 , and IKK beta ... Respiratory syncytial virus mediated NF-kappa B p65 phosphorylation at serine 536 is dependent on RIG-I , TRAF6, and IKK beta ... Respiratory syncytial virus mediated NF-kappa B p65 phosphorylation at serine 536 is dependent on RIG-I, TRAF6, and IKK beta
Byun et al., Mediators Inflamm 2010 (Cholesteatoma, Middle Ear) : We found that CYLD reduced and activated increased NF-kappaB in cholesteatoma epithelium in comparison to RA skin
Li et al., Zhongguo Shi Yan Xue Ye Xue Za Zhi 2010 : The mechanism of apoptosis may be related to the down-regulation of the expression of NF-kappaB and the activation of caspase 3
Ye et al., Oncogene 2010 (Bone Cysts, Aneurysmal) : TRE17 induces transcription of MMP-9 through activation of nuclear factor-kappaB (NF-kappaB) , mediated in part by the GTPase RhoA and its effector kinase, ROCK
Zhang et al., J Med Virol 2010 : Interestingly, we found that the inhibition of nuclear factor-kappaB (NF-kappaB) mediated by siRNA targeting NF-kappaB/p65 mRNA or PDTC ( an inhibitor of NF-kappaB ) could attenuate the upregulation of Capn4 ... While, HBx failed to increase the promoter activity of Capn4 in hepatoma cells when the putative NF-kappaB binding site of the Capn4 promoter was mutant, suggesting that NF-kappaB is involved in the activation of Capn4 mediated by HBx
Choo et al., Rheumatology (Oxford) 2010 (Arthritis, Rheumatoid) : In addition, MS-275 and SAHA suppressed lipopolysaccharide (LPS) induced NF-kappaB p65 nuclear accumulation, IL-6, IL-18 and nitric oxide ( NO ) secretion as well as down-regulated pro-angiogenic VEGF and MMP-2 and MMP-9 production in E11 cells at sub-micromolar levels ... At similar concentrations, MS-275 and SAHA suppressed LPS induced NF-kappaB p65 nuclear accumulation and IL-1beta, IL-6, IL-18 and TNF-alpha secretion in THP-1 monocytic cells
Yang et al., J Biol Chem 2010 (Insulin Resistance...) : Chromatin immunoprecipitation and luciferase reporter assays show that activation of AMPK by CA-alpha1 in macrophages significantly inhibits LPS- or FFA induced NF-kappaB signaling
Valanne et al., J Immunol 2010 : Moreover, small interfering RNA silencing of the human ortholog GRK5 in HeLa cells impaired NF-kappaB reporter activity
Paur et al., Cancer prevention research (Philadelphia, Pa.) 2010 (Inflammation) : A combined extract of clove, oregano, thyme, walnuts, and coffee synergistically inhibited lipopolysaccaride (LPS) induced NF-kappaB activation in a monocytic cell line, compared with the sum of effects from the single extracts ... Based on the area under the curve, the extract decreased whole body LPS induced NF-kappaB activity the first 6 hours by 35 % compared with control mice
Shao et al., J Biol Chem 2010 : Here, we show that nuclear factor kappaB (NF-kappaB) and mitogen activated protein kinase ( MAPK ) signaling pathways regulate the expression of CCAAT/enhancer binding protein homologous protein ( CHOP ), which mediates endoplasmic reticulum stress induced apoptosis
Kannan-Thulasiraman et al., J Biol Chem 2010 (Carcinoma) : In turn, NF-kappaB induces the expression of FABP5 through two cognate response elements in the promoter of this gene
Xu et al., Exp Lung Res 2010 : To evaluate the direct effects of CS and SP on the NK1R expression and the involvement of nuclear factor (NF)-kappaB, macrophages were exposed to CS condensate ( CSC ) and/or SP without or with blocking NK1R or inhibiting NF-kappaB activation in vitro
Li et al., J Virol 2010 : However, knockdown of RIG-I only partially blocked NF-kappaB activity induced by MHV infection and inhibition of NF-kappaB activity by a decoy peptide inhibitor had little effect on IFN-alpha/beta production
Moubarak et al., J Neurosci 2010 : Moreover, NGF dependent activation of two main intracellular pathways involved in the regulation of neurite outgrowth, extracellular signal regulated kinases ( ERKs ) and nuclear factor kappaB (NF-kappaB) , is impaired when endogenous FLIP-L is downregulated, although TrkA remains activated
Priyadarsini et al., Free radical research 2010 : Altered expression of the Bcl-2 family of proteins, inhibition of NF-kappaB activation and over-expression of caspases and survivin provide compelling evidence that azadirachtin and nimbolide induce a shift of balance toward a pro-apoptotic phenotype
Viji et al., Int Immunopharmacol 2010 : These results suggest that betulinic acid inhibited IL-6 production by preventing p65 NF-kappaB nuclear translocation and there is a possibility that this prevention of p65 nuclear translocation may involve p38 and ERK MAPKs as cross talks occur between MAPK and NF-kappaB pathways
Kim et al., Age (Dordrecht, Netherlands) 2010 : It is also known that AGE enhance the generation of RS and that the binding of AGE to a specific AGE receptor ( RAGE ) induces the activation of the redox-sensitive, pro-inflammatory transcription factor , nuclear factor-kappa B (NF-kB) ... It is also known that AGE enhance the generation of RS and that the binding of AGE to a specific AGE receptor ( RAGE ) induces the activation of the redox-sensitive, pro-inflammatory transcription factor, nuclear factor-kappa B (NF-kB) ... It is also known that AGE enhance the generation of RS and that the binding of AGE to a specific AGE receptor ( RAGE ) induces the activation of the redox-sensitive, pro-inflammatory transcription factor, nuclear factor-kappa B (NF-kB)
Trebec-Reynolds et al., J Cell Biochem 2010 : VEGF-A expression in osteoclasts is regulated by NF-kappaB induction of HIF-1alpha ... VEGF-A expression in osteoclasts is regulated by NF-kappaB induction of HIF-1alpha ... In turn, Hif1alpha mRNA levels are induced by RANKL mediated activation of NF-kappaB ... In turn, Hif1alpha mRNA levels are induced by RANKL mediated activation of NF-kappaB
Yang et al., J Cell Physiol 2010 (Arthritis, Rheumatoid) : IL-1beta induced ICAM-1 expression, extracellular signal regulated kinase ( ERK ) and c-Jun-N-terminal kinase (JNK) phosphorylation, AP-1 activation, and nuclear factor-kappaB (NF-kappaB) p65 translocation were attenuated by the inhibitors of MEK1/2 ( U0126 ), JNK ( SP600125 ), AP-1 ( tanshinone IIA ), and NF-kappaB ( helenalin ) or transfection with respective short hairpin RNA plasmids ... IL-1beta induced ICAM-1 expression, extracellular signal regulated kinase ( ERK ) and c-Jun-N-terminal kinase (JNK) phosphorylation, AP-1 activation, and nuclear factor-kappaB (NF-kappaB) p65 translocation were attenuated by the inhibitors of MEK1/2 ( U0126 ), JNK ( SP600125 ), AP-1 ( tanshinone IIA ), and NF-kappaB ( helenalin ) or transfection with respective short hairpin RNA plasmids ... Moreover, IL-1beta stimulated NF-kappaB p65 translocation was blocked by helenalin, but not by U0126 or SP600125, revealing that MAPKs and NF-kappaB pathways were independent on these responses ... Moreover, IL-1beta stimulated NF-kappaB p65 translocation was blocked by helenalin, but not by U0126 or SP600125, revealing that MAPKs and NF-kappaB pathways were independent on these responses ... These results suggest that in human RASFs, activation of ERK, JNK, AP-1, and NF-kappaB are essential for IL-1beta induced ICAM-1 expression and leukocyte adhesion ... IL-1beta induced ICAM-1 expression, extracellular signal regulated kinase ( ERK ) and c-Jun-N-terminal kinase (JNK) phosphorylation, AP-1 activation, and nuclear factor-kappaB (NF-kappaB) p65 translocation were attenuated by the inhibitors of MEK1/2 ( U0126 ), JNK ( SP600125 ), AP-1 ( tanshinone IIA ), and NF-kappaB ( helenalin ) or transfection with respective short hairpin RNA plasmids ... IL-1beta induced ICAM-1 expression, extracellular signal regulated kinase ( ERK ) and c-Jun-N-terminal kinase (JNK) phosphorylation, AP-1 activation, and nuclear factor-kappaB (NF-kappaB) p65 translocation were attenuated by the inhibitors of MEK1/2 ( U0126 ), JNK ( SP600125 ), AP-1 ( tanshinone IIA ), and NF-kappaB ( helenalin ) or transfection with respective short hairpin RNA plasmids ... IL-1beta induced ICAM-1 expression, extracellular signal regulated kinase ( ERK ) and c-Jun-N-terminal kinase (JNK) phosphorylation, AP-1 activation, and nuclear factor-kappaB (NF-kappaB) p65 translocation were attenuated by the inhibitors of MEK1/2 ( U0126 ), JNK ( SP600125 ), AP-1 ( tanshinone IIA ), and NF-kappaB ( helenalin ) or transfection with respective short hairpin RNA plasmids
Han et al., Molecular cancer 2010 (Disease Models, Animal...) : Co-treatment with NF-kappaB , STAT3 or/and PI3K inhibitors led to additive inhibition of iMyc E mu-1 cell proliferation, suggesting that these signaling pathways converge ... Co-treatment with NF-kappaB , STAT3 or/and PI3K inhibitors led to additive inhibition of iMyc E mu-1 cell proliferation, suggesting that these signaling pathways converge ... Co-treatment with NF-kappaB , STAT3 or/and PI3K inhibitors led to additive inhibition of iMyc E mu-1 cell proliferation, suggesting that these signaling pathways converge
Shimada et al., FEBS Lett 2010 : In summary, MCP-1 production was partly regulated by ROCK acting upstream of p38 and JNK and mediated downstream by NF-kappaB ... In summary, MCP-1 production was partly regulated by ROCK acting upstream of p38 and JNK and mediated downstream by NF-kappaB ... In summary, MCP-1 production was partly regulated by ROCK acting upstream of p38 and JNK and mediated downstream by NF-kappaB
Cui et al., Cell 2010 (Inflammation) : NLRC5 negatively regulates the NF-kappaB and type I interferon signaling pathways
Zhang et al., J Biol Chem 2010 (Inflammation) : Lipopolysaccharide, as a potent inducer of IL-27 p28 expression, could activate IRF-8 expression in a MyD88 dependent pathway, which in turn induced p28 gene transcription through NF-kappaB and/or IRF-8
Gray et al., J Immunol 2010 : MD-2s also interacted with LPS and TLR4, but failed to mediate LPS induced NF-kappaB activation and IL-8 production
Zheng et al., J Immunol 2010 : In addition to promoting both MyD88- and TLR/IL-1 receptor domain containing adaptor protein inducing IFN-beta dependent MAPKs and NF-kappaB activation, Gab1 enhances PI3K/Akt activation by directly binding p85 in TLR signaling and VSV infection ... In addition to promoting both MyD88- and TLR/IL-1 receptor domain containing adaptor protein inducing IFN-beta dependent MAPKs and NF-kappaB activation, Gab1 enhances PI3K/Akt activation by directly binding p85 in TLR signaling and VSV infection
Jandhyala et al., Infect Immun 2010 (Escherichia coli Infections) : Using reporter gene constructs, we showed that flagellin mediated interleukin-8 gene transcription is heavily dependent on both NF-kappaB and extracellular signal regulated kinase 1 and 2 ( ERK-1/2 ) activation
Lee et al., Exp Mol Med 2010 : TNF-alpha activated the NF-kappaB and the JNK pathways ... Inhibition of NF-kappaB or JNK activation reduced the inhibitory effect of TNF-alpha on ALP expression, whereas TNF-alpha induced Msx2 expression was only suppressed by the inhibition of the NF-kappaB pathway
Mercatelli et al., Free Radic Biol Med 2010 : This protection did not correlate with the modulation of the expression of VEGF receptors, but is clearly linked to the phosphorylation of the KDR/Flk-1 receptor, the activation of NF-kappaB , and/or the overexpression of the antiapoptotic protein alphaB-crystallin ... This protection did not correlate with the modulation of the expression of VEGF receptors, but is clearly linked to the phosphorylation of the KDR/Flk-1 receptor, the activation of NF-kappaB , and/or the overexpression of the antiapoptotic protein alphaB-crystallin
Harikumar et al., Mol Cancer Ther 2010 (Carcinoma, Pancreatic Ductal...) : Using Panc-1 as a model system, we showed that CRT0066101 reduced bromodeoxyuridine incorporation ; increased apoptosis ; blocked neurotensin induced PKD1/2 activation ; reduced neurotensin induced, PKD mediated Hsp27 phosphorylation ; attenuated PKD1 mediated NF-kappaB activation ; and abrogated the expression of NF-kappaB dependent proliferative and prosurvival proteins
Huang et al., Endocrinology 2010 : NF-kappaB and AP-1 are involved in high-glucose induced PRR up-regulation in rat mesangial cells
Sun et al., J Biol Chem 2010 : MEKK3 is a central intermediate signaling component in lysophosphatidic acid (LPA) induced activation of the nuclear factor-kappaB (NF-kappaB) ... Overexpression of PP2A catalytic subunit (PP2Ac) beta-isoform results in dephosphorylation of MEKK3 at Thr-516 and Ser-520 and termination of MEKK3 mediated NF-kappaB activation ... Furthermore, knockdown of PP2Ac expression enhances LPA induced MEKK3 mediated IkappaB kinase beta (IKKbeta) phosphorylation and NF-kappaB activation ... These data suggest that PP2A plays an important role in the termination of LPA mediated NF-kappaB activation through dephosphorylating and inactivating MEKK3
Jeon et al., Proc Natl Acad Sci U S A 2010 (Pneumonia) : In particular, vinpocetine inhibits TNF-alpha induced NF-kappaB activation and the subsequent induction of proinflammatory mediators in multiple cell types, including vascular smooth muscle cells, endothelial cells, macrophages, and epithelial cells
Bruno et al., Immunol Invest 2010 : Induction of pIgR expression in HT-29 cells required NF-kappaB signaling but not MAPK signaling, in contrast to the requirement for both NF-kappaB and MAPK signaling for induction of pro-inflammatory genes
Barisic et al., Biochem Pharmacol 2010 : While the activation status of Src is Tyr phosphorylation dependent, NFkappaB activation requires Ser phosphorylation of its cytosolic inhibitor, IkappaBalpha
Cuneo et al., J Mol Cell Cardiol 2010 : IL-19 does not inhibit NF-kappaB , but does transiently reduce cytoplasmic abundance of the mRNA stability factor HuR
Feng et al., Int Immunopharmacol 2010 (Disease Models, Animal...) : Meanwhile, HES could significantly reduce TNF-alpha, IL-6, and ICAM-1 mRNA, inhibit NF-kappaB activation, and down-regulate TLR2 and TLR4 expression in the brain ... Meanwhile, HES could significantly reduce TNF-alpha, IL-6, and ICAM-1 mRNA, inhibit NF-kappaB activation, and down-regulate TLR2 and TLR4 expression in the brain ... Meanwhile, HES could significantly reduce TNF-alpha , IL-6, and ICAM-1 mRNA, inhibit NF-kappaB activation, and down-regulate TLR2 and TLR4 expression in the brain ... Meanwhile, HES could significantly reduce TNF-alpha, IL-6 , and ICAM-1 mRNA, inhibit NF-kappaB activation, and down-regulate TLR2 and TLR4 expression in the brain ... Meanwhile, HES could significantly reduce TNF-alpha, IL-6, and ICAM-1 mRNA, inhibit NF-kappaB activation, and down-regulate TLR2 and TLR4 expression in the brain
Twait et al., Pancreatology 2010 (Pancreatitis) : Dominant negative p38 mitogen activated protein kinase expression inhibits NF-kappaB activation in AR42J cells ... Dominant negative p38 mitogen activated protein kinase expression inhibits NF-kappaB activation in AR42J cells
Gupta et al., Neurochem Res 2010 (Parkinson Disease, Secondary...) : The results obtained demonstrate that nitric oxide contributes to an increase of MB- and PQ-induced lipid peroxidation in mouse striatum and tyrosine kinase, p38 MAPK and NF-kB regulate iNOS expression
Manea et al., Biochem Biophys Res Commun 2010 (Atherosclerosis...) : Since nuclear factor-kappaB (NF-kappaB) controls the expression of many genes associated to inflammation related diseases, in this study we have investigated the role of NF-kappaB signaling in the regulation of Nox1 and Nox4 transcription in human aortic smooth muscle cells ( SMCs ) ... Since nuclear factor-kappaB (NF-kappaB) controls the expression of many genes associated to inflammation related diseases, in this study we have investigated the role of NF-kappaB signaling in the regulation of Nox1 and Nox4 transcription in human aortic smooth muscle cells ( SMCs )
Ahmed et al., Molecular cancer 2010 (Breast Neoplasms) : Osteopontin selectively regulates p70S6K/mTOR phosphorylation leading to NF-kappaB dependent AP-1 mediated ICAM-1 expression in breast cancer cells ... Osteopontin selectively regulates p70S6K/mTOR phosphorylation leading to NF-kappaB dependent AP-1 mediated ICAM-1 expression in breast cancer cells ... The data revealed that OPN induces NF-kappaB activation and NF-kappaB dependent ICAM-1 expression ... The data revealed that OPN induces NF-kappaB activation and NF-kappaB dependent ICAM-1 expression ... We also observed that OPN induced NF-kappaB further controls AP-1 transactivation, suggesting that there is cross talk between NF-kappaB and AP-1 which is unidirectional towards AP-1 that in turn regulates ICAM-1 expression in these cells ... We also observed that OPN induced NF-kappaB further controls AP-1 transactivation, suggesting that there is cross talk between NF-kappaB and AP-1 which is unidirectional towards AP-1 that in turn regulates ICAM-1 expression in these cells ... Moreover, overexpression of mTOR inhibits OPN induced NF-kappaB and AP-1-DNA binding and transcriptional activity ... Moreover, overexpression of mTOR inhibits OPN induced NF-kappaB and AP-1-DNA binding and transcriptional activity
Schindler et al., Cell Signal 2010 : In secondary assays, we demonstrated that hexokinase 1 does not alter TNF dependent activation of NF-kappaB or JNK modules
Harikumar et al., Mol Cancer Res 2010 (Neoplasm Invasiveness...) : Sesamin downregulated constitutive and inducible NF-kappaB activation induced by various inflammatory stimuli and carcinogens, and inhibited the degradation of IkappaBalpha , the inhibitor of NF-kappaB , through the suppression of phosphorylation of IkappaBalpha and inhibition of activation of IkappaBalpha protein kinase, thus resulting in the suppression of p65 phosphorylation and nuclear translocation, and NF-kappaB mediated reporter gene transcription
Del Pozo et al., Pancreatology 2010 (Pancreatic Neoplasms...) : Specifically, LPS can activate NF-kappaB signaling in pancreatic cancer cells, thus connecting inflammation with cancer progression
Liu et al., Mol Nutr Food Res 2010 (Adenocarcinoma...) : Nuclear factor (NF)-kappaB inhibitor induced a marked reduction in MMP-9 and MMP-2 expression, suggesting NF-kappaB pathway could play an important role ... Nuclear factor (NF)-kappaB inhibitor induced a marked reduction in MMP-9 and MMP-2 expression, suggesting NF-kappaB pathway could play an important role
Zuo et al., J Proteome Res 2010 (MAP Kinase Signaling System) : 14-3-3 epsilon dynamically interacts with key components of mitogen activated protein kinase signal module for selective modulation of the TNF-alpha induced time course dependent NF-kappaB activity ... We then postulated a mechanistic view describing how 14-3-3 epsilon coordinates its dynamic interactions with TAK1 and PPM1B for differentially modulating TNF-alpha induced changes in NF-kappaB activity
Miguel-Carrasco et al., Journal of inflammation (London, England) 2010 : To understand the mechanisms involved in the effect of captopril, mRNA expression of ACE, angiotensin II type I receptor (AT1R) and p22phox ( a subunit of NADPH oxidase ), as well as NF-kappaB activation and expression, were measured in the left ventricle of these animals ... To understand the mechanisms involved in the effect of captopril, mRNA expression of ACE , angiotensin II type I receptor (AT1R) and p22phox ( a subunit of NADPH oxidase ), as well as NF-kappaB activation and expression, were measured in the left ventricle of these animals ... To understand the mechanisms involved in the effect of captopril, mRNA expression of ACE, angiotensin II type I receptor (AT1R) and p22phox ( a subunit of NADPH oxidase ), as well as NF-kappaB activation and expression, were measured in the left ventricle of these animals
Kim et al., BMC cancer 2010 (Breast Neoplasms...) : Indeed, CARM1 significantly regulated both p53 and NF-kappaB target gene transcription
Gonzalez-Perez et al., Cell Signal 2010 (Mammary Neoplasms, Experimental) : Deletion analysis of VEGF promoter and RNA knockdown shows that HIF-1alpha and NFkappaB are essentials for leptin regulation of VEGF ... Deletion analysis of VEGF promoter and RNA knockdown shows that HIF-1alpha and NFkappaB are essentials for leptin regulation of VEGF
Kim et al., Mediators Inflamm 2010 : Immunoblotting analysis suggested that CA inhibition was due to the inhibition of phosphoinositide-3-kinase (PI3K) and phosphoinositide dependent kinase (PDK)1 as well as nuclear factor-(NF-) kappaB activation ... Immunoblotting analysis suggested that CA inhibition was due to the inhibition of phosphoinositide-3-kinase (PI3K) and phosphoinositide dependent kinase (PDK)1 as well as nuclear factor-(NF-) kappaB activation
Baker et al., Glia 2010 : OSM regulates gene expression primarily by activating the JAK/STAT, NF-kappaB , and/or MAPK pathways, in a cell-type specific manner ... In our studies, OSM induces the production of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS) from microglia in an NF-kappaB dependent manner ... This expression also partially requires the intermediate production of TNF-alpha and subsequent NF-kappaB activation via TNF-R1
Matuschak et al., Respir Physiol Neurobiol 2010 : Acute hypoxia decreases E. coli LPS induced cytokine production and NF-kappaB activation in alveolar macrophages ... Thus, dynamic reductions in the ambient PO ( 2 ) of alveolar macrophages that do not deplete GSH suppress LPS induced TNF-alpha expression, IL-1beta production, and NF-kappaB activation even as oxyradical production is decreased
Bachetti et al., Exp Cell Res 2010 (Astrocytoma...) : Ceftriaxone has also been shown to modulate the proteasome system, thus decreasing NF-kappaB activation and GFAP promoter transcriptional regulation , which further accounts for the down-modulation of GFAP protein levels
Yoshida et al., Nat Med 2010 (Pulmonary Emphysema) : We also found that Rtp801 was necessary and sufficient for nuclear factor-kappaB (NF-kappaB) activation in cultured cells and, when forcefully expressed in mouse lungs, it promoted NF-kappaB activation, alveolar inflammation, oxidative stress and apoptosis of alveolar septal cells
Jing et al., Diabetes Metab Res Rev 2010 (Diabetes Mellitus, Experimental...) : The vascular smooth muscle cell of the RSV treated rats was characterized with less proliferation, lower NF-kappaB , and Erk1/2 activation , decreased proliferation cell nuclear antigen and receptor for advanced glycation end product expression ... The vascular smooth muscle cell of the RSV treated rats was characterized with less proliferation, lower NF-kappaB , and Erk1/2 activation , decreased proliferation cell nuclear antigen and receptor for advanced glycation end product expression
Bettermann et al., Cancer Cell 2010 (Cell Transformation, Neoplastic...) : TAK1 mediated cancer suppression is exerted through activating NF-kappaB in response to tumor necrosis factor (TNF) and through preventing Caspase-3 dependent hepatocyte and cholangiocyte apoptosis
Nam et al., J Nat Prod 2010 : Fijiolides A and B, inhibitors of TNF-alpha induced NFkappaB activation, from a marine derived sediment bacterium of the genus Nocardiopsis ... Fijiolide A reduced TNF-alpha induced NFkappaB activation by 70.3 %, with an IC ( 50 ) value of 0.57 micro-M
Dasgupta et al., Biochem J 2010 (Insulin Resistance) : NF-kappaB mediates lipid induced fetuin-A expression in hepatocytes that impairs adipocyte function effecting insulin resistance
Huang et al., J Immunol 2010 : Blockage of LPS induced ERK, JNK, Akt, and NF-kappaB activation contributed to the inhibitory effect of quercetin on DCs
Green et al., J Immunol 2010 (Tuberculosis, Central Nervous System) : Chemical inhibition and promoter-reporter analyses showed that NF-kappaB and AP-1 c-Jun/FosB heterodimers regulate CoMTb induced MMP-1/-3 secretion ... Chemical inhibition and promoter-reporter analyses showed that NF-kappaB and AP-1 c-Jun/FosB heterodimers regulate CoMTb induced MMP-1/-3 secretion
Zhang et al., Mol Biol Cell 2010 : The scaffold protein TANK/I-TRAF inhibits NF-kappaB activation by recruiting polo-like kinase 1 ... The scaffold protein TANK/I-TRAF inhibits NF-kappaB activation by recruiting polo-like kinase 1 ... The scaffold protein TANK/I-TRAF inhibits NF-kappaB activation by recruiting polo-like kinase 1 ... PLK1 inhibits NF-kappaB transcriptional activation induced by TNF-alpha, IL-1beta, or several activators, but not by nuclear transcription factor p65 ... PLK1 inhibits NF-kappaB transcriptional activation induced by TNF-alpha, IL-1beta, or several activators, but not by nuclear transcription factor p65 ... PLK1 inhibits NF-kappaB transcriptional activation induced by TNF-alpha , IL-1beta, or several activators, but not by nuclear transcription factor p65 ... PLK1 inhibits NF-kappaB transcriptional activation induced by TNF-alpha, IL-1beta , or several activators, but not by nuclear transcription factor p65 ... PLK1 expression reduces the DNA binding activity of NF-kappaB induced by TNF-alpha ... PLK1 expression reduces the DNA binding activity of NF-kappaB induced by TNF-alpha
Usui et al., J Pharmacol Sci 2010 (Atherosclerosis) : NAC inhibited TNF induced phosphorylation of JNK and p38 but not NF-kappaB ... NAC inhibited TNF induced phosphorylation of JNK and p38 but not NF-kappaB
Uyanik et al., Ren Fail 2010 (Body Weight...) : In immunohistochemical staining, we determined that CLP operation increased NF-kappaB activation
Nagarajan et al., Bioorg Med Chem 2010 : IkappaB kinase (IKK) is critical in proinflammatory cytokine induced IkappaBalpha phosphorylation and subsequent activation of the nuclear transcription factor NF-kappaB complex
Ohata et al., Journal of clinical biochemistry and nutrition 2010 : This inhibition likely resulted from the inhibitory effect of PZ on LPS mediated nuclear factor-kappaB (NF-kappaB) activation
Lee et al., Int Immunopharmacol 2010 (Pneumonia) : In contrast, there was no significant difference in levels of NF-kappaB regulated MIP-1 alpha between CC10-Cre ( tg ) /Ikk beta ( Delta/Delta ) and WT mice
Baron et al., PloS one 2010 (Endotoxemia...) : Transfection studies demonstrated a critical role for HMGA1 in facilitating cytokine and NF-kappaB induction of P-selectin promoter activity, and Dist A inhibited binding of HMGA1 to this AT-rich region of the P-selectin promoter in vivo ... Transfection studies demonstrated a critical role for HMGA1 in facilitating cytokine and NF-kappaB induction of P-selectin promoter activity, and Dist A inhibited binding of HMGA1 to this AT-rich region of the P-selectin promoter in vivo
Ouyang et al., Scand J Immunol 2010 : Previous studies demonstrated that blocking IKK2 in bone marrow derived dendritic cells ( BMDC ) by adenoviral transfection with a kinase-defective dominant negative form of IKK2 ( IKK2dn ) could inhibit NF-kappaB activation and impair DC maturation
Im et al., Am J Physiol Gastrointest Liver Physiol 2009 (Colitis, Ulcerative) : B.P. CM also increased NF-kappaB activation, and inhibitors of NF-kappaB suppressed B.P. CM-induced tube formation and IL-8 production
Razani et al., Science signaling 2010 : Here, we describe a mechanism of negative feedback control of noncanonical NF-kappaB signaling that attenuated the stabilization of NF-kappaB inducing kinase (NIK) , the central regulatory kinase of the noncanonical pathway, induced by B cell activating factor receptor ( BAFF-R ) and lymphotoxin beta receptor (LTbetaR) ... Here, we describe a mechanism of negative feedback control of noncanonical NF-kappaB signaling that attenuated the stabilization of NF-kappaB inducing kinase (NIK) , the central regulatory kinase of the noncanonical pathway, induced by B cell activating factor receptor ( BAFF-R ) and lymphotoxin beta receptor (LTbetaR)
Uchino et al., Blood Coagul Fibrinolysis 2010 (Neoplasm Invasiveness...) : NFkappaB dependent regulation of urokinase plasminogen activator by proanthocyanidin-rich grape seed extract : effect on invasion by prostate cancer cells ... Additional studies showed that GSE inhibited DNA binding activity of the transcription factor nuclear factor kappa B (NFkappaB), which in turn decreased NFkappaB dependent uPA transcription
Hong et al., Neuroscience bulletin 2010 (Brain Ischemia) : In addition, p53 disrupts NF-kappaB binding to p300 and blocks NF-kappaB mediated survival signaling
Lee et al., Am J Chin Med 2010 : ABO inhibited the TNF-alpha induced degradation of IkappaB-alpha , an inhibitor of NF-kappaB , by inhibiting the phosphorylation of IkappaB-alpha in HUVEC
Kadoki et al., Int Immunol 2010 (Acquired Immunodeficiency Syndrome) : p38 mitogen activated protein kinase ( MAPK ) and nuclear factor kappa B (NF-kappaB) activation , but neither extracellular signal regulated kinase nor c-Jun N-terminal kinase activation, were required for the activation, because only inhibitors for p38 MAPK and NF-kappaB suppressed activation of HIV-1
Sun et al., J Virol 2010 : The result showed that certain mutations lethal to virus replication impaired the ability of nsp2 to inhibit NF-kappaB activation but that the viable recombinant viruses, vSD-S462A and vSD-D465A, were unable to inhibit NF-kappaB activation as effectively as the wild-type virus
He et al., J Cell Biochem 2010 : The activation of TLR4 by LPS modulated the expression of TLRs, induced the phosphorylation of NF-kappaB , P38, and ERK42/44, and up-regulated the gene expression of cytokines IL-8, IFN-alpha, IFN-beta, and TNF-alpha, suggesting EPCs expressed functional TLR4 ... The activation of TLR4 by LPS modulated the expression of TLRs, induced the phosphorylation of NF-kappaB , P38, and ERK42/44, and up-regulated the gene expression of cytokines IL-8, IFN-alpha, IFN-beta, and TNF-alpha, suggesting EPCs expressed functional TLR4
Khalaf et al., BMC immunology 2010 (Inflammation) : The present study shows that NF-kappaB regulated IL-6 but not CXCL8 ... The present study shows that NF-kappaB regulated IL-6 but not CXCL8
Grotterød et al., BMC cancer 2010 (Osteosarcoma) : Signal transduction mechanisms involved in S100A4 induced activation of the transcription factor NF-kappaB ... The metastasis promoting protein S100A4 activates the transcription factor NF-kappaB through the classical NF-kappaB activation pathway ... The Ser/Thr kinase inhibitors H-7 and staurosporine inhibited S100A4 induced IkappaBalpha phosphorylation and subsequent NF-kappaB activation ... Dominant negative MEKK1 or NIK did not inhibit S100A4 induced NF-kappaB activity, and S100A4 stimulation did not influence AKT phosphorylation ... Dominant negative MEKK1 or NIK did not inhibit S100A4 induced NF-kappaB activity, and S100A4 stimulation did not influence AKT phosphorylation ... S100A4 activates NF-kappaB by inducing phosphorylation of IKKalpha/beta, leading to increased IkappaBalpha phosphorylation ... The Ser/Thr kinase inhibitors H-7 and staurosporine attenuated S100A4 induced NF-kappaB activation and inhibited IKK mediated phosphorylation of IkappaBalpha ... The Ser/Thr kinase inhibitors H-7 and staurosporine attenuated S100A4 induced NF-kappaB activation and inhibited IKK mediated phosphorylation of IkappaBalpha ... S100A4 induced NF-kappaB activation was independent of the putative S100 protein receptor RAGE and the Ser/Thr kinases MEKK1, NIK and AKT ... S100A4 induced NF-kappaB activation was independent of the putative S100 protein receptor RAGE and the Ser/Thr kinases MEKK1, NIK and AKT
Huang et al., Biomaterials 2010 (Melanoma) : Further results demonstrated that the upregulation of anti-apoptotic molecules Bcl-2 expression and the inhibition of NF-kappaB activation by MSNs may promote cell proliferation in a redox-sensitive signal pathway
D'Alessio et al., J Biol Chem 2010 : We previously reported that methyl-beta-cyclodextrin eliminates caveolae and blocks tumor necrosis factor (TNF) induced internalization of TNFR1 but not TNF induced activation of NF-kappaB in EA.hy926 cells ... Both knockdowns reduce total TNFR1 protein expression, but neither prevents TNFR1 localization to low density membrane domains, TNF induced internalization of TNFR1, or NF-kappaB activation by TNF ... Both knockdowns reduce total TNFR1 protein expression, but neither prevents TNFR1 localization to low density membrane domains, TNF induced internalization of TNFR1 , or NF-kappaB activation by TNF
Lee et al., J Immunol 2010 : Inhibition of MMP-3 or -9 also suppressed the activities of MAPK, NF-kappaB , and AP-1
Li et al., Cell Physiol Biochem 2010 : Oxidized low-density lipoprotein induced proinflammatory cytokine response in macrophages are suppressed by CD4CD25 ( + ) Foxp3 ( + ) regulatory T cells through downregulating toll like receptor 2-mediated activation of NF-kappaB
Srikrishna et al., J Cell Biochem 2010 : mAbGB3.1, an antibody against carboxylated glycans, blocks S100A12 mediated NF-kappaB signaling in HeLa cells expressing full-length RAGE
Zhang et al., Oncol Rep 2010 (Brain Neoplasms...) : Further, down-regulation of AKT2 expression by antisense AKT2 induced glioma cell apoptosis mediated by NFkappaB and BCL2
Laparra Llopis et al., J Physiol Biochem 2010 : These results link the gliadin derived peptides induced TNFalpha production through cAMP dependent PKA activation, where ion channels controlling calcium influx into cells could play a protective role, and requires NF-kappaB activation
Moon et al., Mol Cancer Ther 2010 (Carcinoma, Hepatocellular...) : Indeed, butein repressed the TRAIL mediated activation of NF-kappaB and decreased its transcriptional activity
Wang et al., J Biol Chem 2010 : Shear induced interleukin-6 synthesis in chondrocytes : roles of E prostanoid ( EP ) 2 and EP3 in cAMP/protein kinase A- and PI3-K/Akt dependent NF-kappaB activation ... Shear induced interleukin-6 synthesis in chondrocytes : roles of E prostanoid ( EP ) 2 and EP3 in cAMP/protein kinase A- and PI3-K/Akt dependent NF-kappaB activation
Xu et al., Eur J Pharmacol 2010 (Inflammation) : In addition, icariin suppressed the secretion of TNF-alpha , prostaglandin E2 ( PGE ( 2 ) ) and nitric oxide ( NO ) as well as NF-kappaB p65 activation
Sekine et al., Yakugaku Zasshi 2010 : Moreover, STAP-2 interacts with Epstein-Barr virus derived LMP1 and modulates LMP1 mediated NF-kappaB signaling
Hamoudi et al., Leukemia 2010 (Lymphoma, B-Cell, Marginal Zone...) : Finally, expression of TLR6 , in the presence of TLR2, enhanced both API2-MALT1 and BCL10 mediated NF-kappaB activation in vitro
Zak et al., Leukemia 2010 (Lymphoma, Mantle-Cell) : Fenofibrate also decreased the nuclear translocation of nuclear factor (NF)-kappaB-p65 and significantly inhibited the DNA binding of NF-kappaB in a dose dependent manner
Xie et al., PloS one 2010 (Breast Neoplasms...) : NF-kappaB inhibition attenuated STAT3 and Smad3 activities whereas PGN-SA stimulated cell culture supernatants reversed these inhibitory effects ... NF-kappaB inhibition attenuated STAT3 and Smad3 activities whereas PGN-SA stimulated cell culture supernatants reversed these inhibitory effects
Lee et al., J Med Food 2010 (MAP Kinase Signaling System) : We also found that the cell based assay system showed that HAK suppressed LPS induced NF-kappaB activity in transfectant RAW 264.7 cells
Han et al., Biol Pharm Bull 2010 : Moreover, compounds 1 and 2 attenuated NF-kappaB activation by inhibition of IkappaBalpha proteolysis and p65 nuclear translocation, as well as phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2, c-Jun N-terminal kinase (JNK), and p38 MAP kinases ... Moreover, compounds 1 and 2 attenuated NF-kappaB activation by inhibition of IkappaBalpha proteolysis and p65 nuclear translocation, as well as phosphorylation of extracellular signal regulated kinase ( ERK ) 1/2, c-Jun N-terminal kinase (JNK), and p38 MAP kinases
Homma et al., Int Arch Allergy Immunol 2010 : In this mechanism, TNF-alpha may activate NF-kappaB , in cooperation with TLR3 signaling
Larner-Svensson et al., Respir Res 2010 : We have shown that IL-1beta induced miR-146a expression in HASM and that this was regulated at the transcriptional level by NF-kappaB and at the post-transcriptional level by the MEK-1/2 and JNK-1/2 ... We have shown that IL-1beta induced miR-146a expression in HASM and that this was regulated at the transcriptional level by NF-kappaB and at the post-transcriptional level by the MEK-1/2 and JNK-1/2 ... We have shown that IL-1beta induced miR-146a expression in HASM and that this was regulated at the transcriptional level by NF-kappaB and at the post-transcriptional level by the MEK-1/2 and JNK-1/2
Zahlten et al., J Immunol 2010 (Disease Models, Animal...) : KLF2 dependent downregulation of NF-kappaB activity is partly reversible by overexpression of the histone acetylase p300/CREB binding protein associated factor
Antonios et al., J Immunol 2010 (MAP Kinase Signaling System) : Our results showed that p38 MAPK, NF-kappaB , and IRF-1 were involved in IL-12p40 production induced by NiSO ( 4 ) ... Our results showed that p38 MAPK, NF-kappaB , and IRF-1 were involved in IL-12p40 production induced by NiSO ( 4 )
Hoshino et al., J Med Chem 2010 : Five resveratrol sulfate metabolites were synthesized and assessed for activities known to be mediated by resveratrol : inhibition of tumor necrosis factor (TNF) alpha induced NFkappaB activity, cylcooxygenases ( COX-1 and COX-2 ), aromatase, nitric oxide production in endotoxin stimulated macrophages, proliferation of KB or MCF7 cells, induction of quinone reductase 1 (QR1), accumulation in the sub-G ( 1 ) phase of the cell cycle, and quenching of 2,2-diphenyl-1-picrylhydrazyl ( DPPH ) free radical ... Five resveratrol sulfate metabolites were synthesized and assessed for activities known to be mediated by resveratrol : inhibition of tumor necrosis factor (TNF) alpha induced NFkappaB activity, cylcooxygenases ( COX-1 and COX-2 ), aromatase, nitric oxide production in endotoxin stimulated macrophages, proliferation of KB or MCF7 cells, induction of quinone reductase 1 (QR1) , accumulation in the sub-G ( 1 ) phase of the cell cycle, and quenching of 2,2-diphenyl-1-picrylhydrazyl ( DPPH ) free radical ... Two metabolites showed activity in these assays ; the 3-sulfate exhibited QR1 induction, DPPH free radical scavenging, and COX-1 and COX-2 inhibitory activities and the 4'-sulfate inhibited NFkappaB induction, as well as COX-1 and COX-2 activities ... Two metabolites showed activity in these assays ; the 3-sulfate exhibited QR1 induction, DPPH free radical scavenging, and COX-1 and COX-2 inhibitory activities and the 4'-sulfate inhibited NFkappaB induction, as well as COX-1 and COX-2 activities ... Two metabolites showed activity in these assays ; the 3-sulfate exhibited QR1 induction, DPPH free radical scavenging, and COX-1 and COX-2 inhibitory activities and the 4'-sulfate inhibited NFkappaB induction, as well as COX-1 and COX-2 activities
Zhao et al., J Biol Chem 2010 : CC2D1A , a DM14 and C2 domain protein, activates NF-kappaB through the canonical pathway ... Here, we show that CC2D1A is a potent activator of NF-kappaB ... The activation of NF-kappaB by CC2D1A requires its C2 domain ... CC2D1A activates NF-kappaB in a manner that depends on the ubiquitin conjugating enzyme Ubc13, TNF receptor associated factor TRAF2, the protein kinase TAK1, and the IkappaB kinase (IKK) complex
Gautheron et al., Hum Mol Genet 2010 (Incontinentia Pigmenti) : In contrast, activation of NF-kappaB by TNF-alpha was not affected
Yang et al., J Clin Invest 2010 (Melanoma...) : Together, our findings provide genetic and mechanistic evidence that mutant HRas initiation of tumorigenesis requires Ikkbeta mediated NF-kappaB activity
Liu et al., Proc Natl Acad Sci U S A 2010 (Helicobacter Infections) : Together these observations indicate that OLFM4 exerts considerable influence on the host defense against H. pylori infection acting through NOD1 and NOD2 mediated NF-kappaB activation and subsequent cytokines and chemokines production, which in turn inhibit host immune response and contribute to persistence of H. pylori colonization
Coles et al., Proc Natl Acad Sci U S A 2010 (Inflammation) : We have generated Ing4-deficient mice to explore the role of Ing4 in development, tumorigenesis, and in NF-kappaB signaling ... Thus, Ing4 negatively regulates the cytokine mediated inflammatory response in mice by facilitating NF-kappaB activation of IkappaB promoters, thereby suppressing nuclear RelA levels and the activation of select NF-kappaB target cytokines
Darwech et al., J Biol Chem 2010 : The IkappaB kinase (IKK) complex , which contains IKKalpha, IKKbeta, and IKKgamma, is required for activation of NF-kappaB , and deletion of either IKKalpha or IKKbeta resulted with defective osteoclast differentiation and survival
Venkatesan et al., J Mol Cell Cardiol 2010 : EMMPRIN activates multiple transcription factors in cardiomyocytes, and induces interleukin-18 expression via Rac1 dependent PI3K/Akt/IKK/NF-kappaB andMKK7/JNK/AP-1 signaling ... We show for the first time that EMMPRIN stimulates the activation of NF-kappaB , AP-1, CREB, and ATF-2 in cardiomyocytes, and induces IL-18 expression via Rac1 dependent PI3K/Akt/IKK/NF-kappaB and MKK7/JNK/AP-1 signaling ... We show for the first time that EMMPRIN stimulates the activation of NF-kappaB , AP-1, CREB, and ATF-2 in cardiomyocytes, and induces IL-18 expression via Rac1 dependent PI3K/Akt/IKK/NF-kappaB and MKK7/JNK/AP-1 signaling ... We show for the first time that EMMPRIN stimulates the activation of NF-kappaB, AP-1, CREB, and ATF-2 in cardiomyocytes, and induces IL-18 expression via Rac1 dependent PI3K/Akt/IKK/NF-kappaB and MKK7/JNK/AP-1 signaling
Jutooru et al., J Biol Chem 2010 (Pancreatic Neoplasms) : Results of Sp1, Sp3, and Sp4 knockdown by RNA interference demonstrate that both p50 and p65 are Sp-regulated genes and that inhibition of constitutive or tumor necrosis factor induced NFkappaB by curcumin is dependent on down-regulation of Sp1 , Sp3, and Sp4 proteins by this compound ... Results of Sp1, Sp3, and Sp4 knockdown by RNA interference demonstrate that both p50 and p65 are Sp-regulated genes and that inhibition of constitutive or tumor necrosis factor induced NFkappaB by curcumin is dependent on down-regulation of Sp1, Sp3 , and Sp4 proteins by this compound ... Results of Sp1, Sp3, and Sp4 knockdown by RNA interference demonstrate that both p50 and p65 are Sp-regulated genes and that inhibition of constitutive or tumor necrosis factor induced NFkappaB by curcumin is dependent on down-regulation of Sp1, Sp3, and Sp4 proteins by this compound
Bi et al., J Biol Chem 2010 : The scaffold protein CARD9 plays an essential role in anti-fungus immunity and is implicated in mediating Dectin-1/Syk induced NF-kappaB activation in response to Candida albicans infection ... The scaffold protein CARD9 plays an essential role in anti-fungus immunity and is implicated in mediating Dectin-1/Syk induced NF-kappaB activation in response to Candida albicans infection ... However, the molecular mechanism by which CARD9 mediates C. albicans induced NF-kappaB activation is not fully characterized ... Here we demonstrate that CARD9 is involved in mediating NF-kappaB activation induced by the hyphal form of C. albicans hyphae ( Hyphae ) but not by its heat inactivated unicellular form ... Our data show that inhibiting Dectin-2 expression selectively blocked Hyphae induced NF-kappaB, whereas inhibiting Dectin-1 mainly suppressed zymosan induced NF-kappaB , indicating that Hyphae induced NF-kappaB activation is mainly through Dectin-2 and not Dectin-1 ... Finally, we find that although both CARD9 and Syk are required for Hyphae induced NF-kappaB activation, they regulate different signaling events in which CARD9 mediates IkappaBalpha kinase ubiquitination, whereas Syk regulates IkappaBalpha kinase phosphorylation ... Finally, we find that although both CARD9 and Syk are required for Hyphae induced NF-kappaB activation, they regulate different signaling events in which CARD9 mediates IkappaBalpha kinase ubiquitination, whereas Syk regulates IkappaBalpha kinase phosphorylation ... Together, our data demonstrated that CARD9 is selectively involved in Dectin-2 induced NF-kappaB activation in response to C. albicans hyphae challenging ... Together, our data demonstrated that CARD9 is selectively involved in Dectin-2 induced NF-kappaB activation in response to C. albicans hyphae challenging
Jiang et al., J Mol Endocrinol 2010 : LPS significantly enhances MCP-1 production and this is dependent on nuclear factor kappa B (NFkappaB) signaling, since the use of NFkappaB inhibitor pyrrolidine dithiocarbamate or the silencing of NFkappaB subunit p65 expression with specific siRNA largely impairs LPS enhanced MCP-1 production ... LPS significantly enhances MCP-1 production and this is dependent on nuclear factor kappa B (NFkappaB) signaling, since the use of NFkappaB inhibitor pyrrolidine dithiocarbamate or the silencing of NFkappaB subunit p65 expression with specific siRNA largely impairs LPS enhanced MCP-1 production
Tanaka et al., Curr Drug Metab 2010 : We found that either UVB, IL-1 or TNFalpha could induce NF-kappaB by activating its signal transduction pathway ... We found that either UVB, IL-1 or TNFalpha could induce NF-kappaB by activating its signal transduction pathway ... We found that either parthenolide or magnolol could effectively inhibit the gene expression mediated by NF-kappaB and the production of bFGF and MMP-1 from cells overexpressing p65, a major subunit of NF-kappaB ... We found that either parthenolide or magnolol could effectively inhibit the gene expression mediated by NF-kappaB and the production of bFGF and MMP-1 from cells overexpressing p65, a major subunit of NF-kappaB
Chen et al., J Biomed Sci 2010 (Burns) : Commensal depletion decreased TLR4 expression as well as NF-kappaB activation of intestine, myeloperoxidase (MPO) activity as well as TNFalpha expression of lung, and bacterial killing activity of peritoneal cells ... Commensal depletion decreased TLR4 expression as well as NF-kappaB activation of intestine, myeloperoxidase (MPO) activity as well as TNFalpha expression of lung, and bacterial killing activity of peritoneal cells
Feng et al., Mol Immunol 2010 (Multiple Myeloma) : Pharmacological inhibition of the transcription factor, NF-kappaB also profoundly decreased TRAIL expression, suggesting that NF-kappaB is involved in the regulation of TRAIL expression in activated human NK cells
Xu et al., PloS one 2010 : Reduction of CFTR expression in AM resulted in increased secretion of IL-8, increased phosphorylation of NF-kappaB , a positive regulator of IL-8 expression, and decreased expression of IkappaB-alpha, the inhibitory protein of NF-kappaB activation ... Reduction of CFTR expression in AM resulted in increased secretion of IL-8, increased phosphorylation of NF-kappaB , a positive regulator of IL-8 expression, and decreased expression of IkappaB-alpha, the inhibitory protein of NF-kappaB activation
Wan et al., Nucleosides Nucleotides Nucleic Acids 2010 : Activation of Stat1, IRF-1, and NF-kappaB is required for the induction of uridine phosphorylase by tumor necrosis factor-alpha and interferon-gamma ... Exposure to IFN-gamma mainly affects nuclear IRF-1 and STAT1 in EMT6, but inhibits NF-kappaB p65 activity, indicating that the cooperative stimulation was the result of the independent activation of NF-kappaB, STAT1 and IRF-1 transcriptional factors through binding to their unique sites in the UPase promoter ... Exposure to IFN-gamma mainly affects nuclear IRF-1 and STAT1 in EMT6, but inhibits NF-kappaB p65 activity, indicating that the cooperative stimulation was the result of the independent activation of NF-kappaB, STAT1 and IRF-1 transcriptional factors through binding to their unique sites in the UPase promoter
Zawawi et al., J Periodontal Res 2010 : Blockage of moesin function interrupts the LPS response through an inhibition of MyD88, IRAK and TRAF6, negatively affecting subsequent activation of the MAP kinases ( p38 and ERK ), NF-kappaB activation and translocation to the nucleus ... Blockage of moesin function interrupts the LPS response through an inhibition of MyD88, IRAK and TRAF6, negatively affecting subsequent activation of the MAP kinases ( p38 and ERK ), NF-kappaB activation and translocation to the nucleus
Shin et al., Int Immunopharmacol 2010 : In addition, sulfuretin attenuated LPS induced DNA binding and the transcriptional activities of nuclear factor-kappa B (NF-kappaB) , which was accompanied by a parallel reduction of degradation and phosphorylation of inhibitory kappa B-alpha ( I kappaB-alpha ) and consequently by decreased nuclear translocation of p65 subunit of NF-kappaB
Pan et al., Zhong Xi Yi Jie He Xue Bao 2010 (Colitis) : Up-regulating the expression of occludin and down regulating the expressions of TLR4 and NF-kappaB , and hence inhibiting TNF-alpha expression and improving the mucosa barrier function may be part of the mechanisms of BWXLS in treating oxazolone induced colitis in mice
Carvalho et al., J Cell Sci 2010 : Although the mechanisms regulating recruitment and activation of IKK within the CBM microenvironment have been extensively studied, there is little understanding of how IKK subsequently binds and phosphorylates IkappaBalpha , the inhibitor of NFkappaB , to promote IkappaBalpha ubiquitylation and proteasomal degradation
Rajaram et al., J Immunol 2010 : The induced IL-8 response mediated by mannose capped lipoarabinomannan and the mannose receptor is independent of TLR2 and NF-kappaB activation
Wang et al., Mucosal Immunol 2010 : Induction of MKP-1 is blocked by inhibitors of nuclear factor (NF)-kappaB , but not of MAPK ... Induction of MKP-1 is blocked by inhibitors of nuclear factor (NF)-kappaB , but not of MAPK ... Thus, NF-kappaB dependent expression of MKP-1 may contribute, by desensitization of p38, to the rapid establishment of unresponsiveness to several TLR ligands in enterocytes
Fang et al., Can J Physiol Pharmacol 2010 : In addition, PGE1 suppressed TNF induced NF-kappaB activation and production of reactive oxygen species
Liu et al., Cell Immunol 2010 (Inflammation) : Further studies have shown that HSP27 regulated LPS induced activation of NF-kappaB by interacting with TRAF6 and increasing the association of TRAF6-IKKgamma ... Further studies have shown that HSP27 regulated LPS induced activation of NF-kappaB by interacting with TRAF6 and increasing the association of TRAF6-IKKgamma
Noman et al., Cell Immunol 2010 (Inflammation) : LPS significantly augmented the expression of RIZ1 and the augmentation was mediated by the activation of nuclear factor (NF)-kappaB and Akt ... Therefore, RIZ1 was suggested to augment LPS induced NF-kappaB activation in collaboration with p53 and enhance the production of proinflammatory cytokines in response to LPS
Steiner et al., Cell Immunol 2010 : PKD1 and PKD2 inhibition reduced NF-kappaB activity but not MAPK activation
Gupta et al., Biochimie 2010 : To elucidate the molecular mechanism of inhibition of cell adhesion molecules, we investigated the status of nuclear transcription factor-kappaB ( NF-kappaB ) and were able to establish that beta-sitosterol significantly blocked the TNFalpha induced activation of NF-kappaB
Chen et al., J Biol Chem 2010 (Colorectal Neoplasms) : rHSP90alpha induced the activities of ERK, PI3K/Akt, and NF-kappaB p65, but only NF-kappaB activation was involved in HSP90alpha induced integrin alpha(V) expression ... rHSP90alpha induced the activities of ERK, PI3K/Akt, and NF-kappaB p65 , but only NF-kappaB activation was involved in HSP90alpha induced integrin alpha(V) expression ... rHSP90alpha induced the activities of ERK , PI3K/Akt, and NF-kappaB p65, but only NF-kappaB activation was involved in HSP90alpha induced integrin alpha(V) expression ... rHSP90alpha induced the activities of ERK, PI3K/Akt , and NF-kappaB p65, but only NF-kappaB activation was involved in HSP90alpha induced integrin alpha(V) expression ... rHSP90alpha induced the activities of ERK, PI3K/Akt , and NF-kappaB p65, but only NF-kappaB activation was involved in HSP90alpha induced integrin alpha(V) expression ... rHSP90alpha induced the activities of ERK, PI3K/Akt , and NF-kappaB p65, but only NF-kappaB activation was involved in HSP90alpha induced integrin alpha(V) expression
Gao et al., J Biol Chem 2010 (Breast Neoplasms) : A-kinase interacting protein 1 (AKIP1) acts as a molecular determinant of PKA in NF-kappaB signaling ... In this study, we have demonstrated the effect of PKA on NF-kappaB activity in view of AKIP1 action ; and in 293 and HeLa cells, where the endogenous AKIP1 expression is minimal, PKA activating agents inhibited the NF-kappaB dependent reporter gene expression, blocked the interaction of PKAc and p65 subunit of NF-kappaB, and attenuated PKA dependent phosphorylation of p65 on Ser-276 ... In the breast cancer cell line, MDA-MB231 cells and MCF7 cells, where the endogenous AKIP1 is abundant, the PKA signal was found to be synergized with NF-kappaB activation ; PKA activating agents enhanced NF-kappaB dependent transcriptional activity and the interaction between p65 and PKAc and augmented the phosphorylation of p65 on Ser-276
Tsubosaka et al., J Pharmacol Sci 2010 (Atherosclerosis) : Herein, we provided the first evidence that halichlorine inhibits LPS induced NF-kappaB activation, which results in the suppression of VCAM-1, ICAM-1, and E-selectin gene expression and monocyte-adhesion to endothelial cells
Goldfeld et al., J Exp Med 1991 : Therefore, TNF-alpha mRNA induction by PMA, like its induction by virus and LPS, is not primarily mediated by NF-kappa B , but rather is mediated through other sequences and protein factors
Li et al., J Clin Immunol 2010 (Arthritis, Rheumatoid...) : The association between IkappaBalpha promoter polymorphisms and disease severity of rheumatoid arthritis is partly due to different transcriptional activities of IkappaBalpha promoter and the activation of NF-kappaB
Mott et al., J Cell Biochem 2010 : Finally, activation of NF-kappaB signaling, via ligation of Toll-like receptors, also repressed mir-29b expression and promoter function
Lixuan et al., Pulm Pharmacol Ther 2010 (Disease Models, Animal...) : The results show that CSE resulted in increasing IL-8, IL-6 and TNF-alpha expression and activation of NF-kappaB ... The results show that CSE resulted in increasing IL-8, IL-6 and TNF-alpha expression and activation of NF-kappaB ... The results show that CSE resulted in increasing IL-8 , IL-6 and TNF-alpha expression and activation of NF-kappaB
Wu et al., Am J Pathol 2010 (Salmonella Infections, Animal) : VDR negatively regulates bacterial induced intestinal NF-kappaB activation and attenuates response to infection
Al-Lamki et al., Am J Pathol 2010 (Carcinoma, Renal Cell) : TNFR1 , expressed in normal kidney primarily on endothelial cells, activates apoptotic signaling kinase 1 and nuclear factor-kappaB (NF-kappaB) and induces cell death, whereas TNFR2, inducibly expressed on endothelial cells and on TECs by injury, activates endothelial/epithelial tyrosine kinase (Etk), which trans-activates vascular endothelial growth factor receptor 2 ( VEGFR2 ) to promote cell proliferation ... In ccRCC organ cultures, R1-TNF increases TNFR1, activates apoptotic signaling kinase and NF-kappaB , and promotes apoptosis in malignant TECs ... R2-TNF increases TNFR2 , activates NF-kappaB , Etk, and VEGFR2 and increases entry into the cell cycle
Tamai et al., Lab Invest 2010 : Conversely, treatment of adipocytes with a PPARgamma antagonist enhanced NF-kappaB activation and conferred resistance to TNF-alpha induced apoptosis, suggesting involvement of PPARgamma in the suppression of NF-kappaB in adipocytes ... Furthermore, transfection with siRNA for C/EBPalpha or C/EBPbeta enhanced activity of NF-kappaB in adipocytes, suggesting that C/EBP is also involved in the repression of NF-kappaB in adipocytes
Xie et al., Clin Hemorheol Microcirc 2010 : We further examined the molecular mechanisms and found that the combination of Sal B and LSS treatment dramatically inhibited TNF-alpha induced NF-kappaB activation evidenced by IkappaBalpha degradation and p65 nuclear translocation in HAECs
Blom et al., BMC cell biology 2010 : We found that S1P protected cells from FasL induced cell death in an NF-kappaB dependent manner
Bradley et al., Mol Endocrinol 2010 : Conversely, Wnt5a represses chondrocyte hypertrophy via NF-kappaB dependent inhibition of Runx2 expression
Prasad et al., J Biol Chem 2010 (Neoplasm Invasiveness...) : Crotepoxide inhibition of NF-kappaB was not inducer-specific ; it inhibited NF-kappaB activation induced by TNF , phorbol 12-myristate 13-acetate, lipopolysaccharide, and cigarette smoke
Gudkov et al., J Clin Invest 2010 : Progress in understanding differences in the mechanisms involved in the responses of normal and tumor cells to genotoxic stress has led to the development of new rational approaches to selective protection of normal cells, such as suppression of apoptosis by pharmacological inhibition of p53 or activation of NF-kappaB
Alvarez et al., Nature 2010 : Genetic evidence indicates that TRAF2 is necessary for the polyubiquitination of receptor interacting protein 1 (RIP1) that then serves as a platform for recruitment and stimulation of IkappaB kinase, leading to activation of the transcription factor NF-kappaB
Knorr et al., Neurosci Lett 2010 (Fever...) : Local MALP-2-treatment induced a moderate STAT3 activation and a small but significant increase in COX-2 IR while no NFkappaB-activation could be observed in the brains of these animals
Tay et al., Nature 2010 : We developed a stochastic mathematical model that reproduces both the digital and analogue dynamics as well as most gene expression profiles at all measured conditions, constituting a broadly applicable model for TNF-alpha induced NF-kappaB signalling in various types of cells
Chaurasia et al., Mol Cell Biol 2010 (Sepsis) : These experiments reveal a novel PDK-1 dependent negative feedback inhibition of TLR induced NF-kappaB activation in macrophages in vivo ... These experiments reveal a novel PDK-1 dependent negative feedback inhibition of TLR induced NF-kappaB activation in macrophages in vivo
Song et al., Virus Genes 2010 : Roles of TRAF2 and TRAF3 in Epstein-Barr virus latent membrane protein 1-induced alternative NF-kappaB activation ... Roles of TRAF2 and TRAF3 in Epstein-Barr virus latent membrane protein 1-induced alternative NF-kappaB activation ... Epstein-Barr virus ( EBV ) latent membrane protein 1 (LMP1) induced NF-kappaB activation is essential for EBV transformed B cell survival ... Using IKKalpha ( -/- ), IKKbeta ( -/- ), IKKgamma ( -/- ), TRAF2 ( -/- ), TRAF3 ( -/- ), TRAF6 ( -/- ), and NIK ( aly/aly ) mouse embryonic fibroblasts ( MEFs ), potential roles of these proteins in LMP1 induced alternative NF-kappaB activation were investigated
Kato et al., Glycoconj J 2010 : SIGNR1 ligation on murine peritoneal macrophages induces IL-12 production through NFkappaB activation ... These results indicate that SIGNR1 mediated intercellular signaling may induce production of cytokines such as IL-12 through NFkappaB activation
Hong et al., Scand J Immunol 2010 : Through ERK inhibitor ( U0126 ) and NF-kB inhibitor ( caffeine acid phenethyl ester ) treatment, it was proven that ERK and NF-kB regulated chitinase induced IL-8 expression
Dudek et al., J Virol 2010 : Rather, PS-341 treatment resulted in an induction of IkappaB degradation and activation of NF-kappaB as well as the JNK/AP-1 pathway
Hattori et al., Diabetologia 2010 : We therefore examined the effect of liraglutide on TNFalpha induced NF-kappaB activation and NF-kappaB dependent expression of proinflammatory genes ... Liraglutide induced enhancement of nitric oxide production and suppression of NF-kappaB activation were attenuated by the AMP activated protein kinase (AMPK) inhibitor compound C or AMPK ( also known as PRKAA1 ) small interfering RNA
Jafri et al., J Exp Clin Cancer Res 2010 (Carcinoma, Non-Small-Cell Lung...) : Inhibition of LPS induced NF-kappaB expression by TQ was determined using transgenic mice expressing a luciferase reporter
Suzuki et al., J Pharmacol Sci 2010 (Brain Ischemia...) : MMP-3 was upregulated by t-PA in endothelial cells, but the upregulation was prevented by the inhibition of either low-density lipoprotein receptor related protein ( LRP ) or nuclear factor kappa-B (NF-kappaB) activation
Li et al., Int J Mol Med 2010 : Pre-treatment of NS3 protein expressing cells with ERK inhibitor, PD98059, blocked the activation of AP-1 and NF-kappaB , and inhibited cyclin D1 expression and cell proliferation
Yamashiro et al., Biochem Biophys Res Commun 2010 : In addition to Ca2+ mobilization through the IP3-receptor, TRPV2 mediated intracellular Ca2+ mobilization is involved in NFkappaB dependent TNFalpha and IL-6 expression, while extracellular Ca2+ entry is involved in NFkappaB independent IL-6 production ... In addition to Ca2+ mobilization through the IP3-receptor, TRPV2 mediated intracellular Ca2+ mobilization is involved in NFkappaB dependent TNFalpha and IL-6 expression, while extracellular Ca2+ entry is involved in NFkappaB independent IL-6 production
Yadav et al., Biochem Pharmacol 2010 : Using the electrophoretic mobility shift assay, we found that CDC was more active than free curcumin in inhibiting TNF induced activation of the inflammatory transcription factor NF-kappaB and in suppressing gene products regulated by NF-kappaB, including those involved in cell proliferation ( cyclin D1 ), invasion ( MMP-9 ), and angiogenesis ( VEGF )
Lee et al., Eur J Pharmacol 2010 : These data suggest that CAPE inhibits TNF-alpha dependent NFkappaB activation via direct inhibition of IKK as well as activation of Nrf2 pathway, in which the functional groups in CAPE may be involved
Wang et al., FEBS Lett 2010 : Here, we investigate the effects of wild-type and mutant p53, and nuclear factor kappa-B (NF-kappaB) ( p50 ) on BCRP promoter activity in MCF-7 cells
Choi et al., Food Chem Toxicol 2010 (Sarcoma) : Moreover, IL-1beta induced activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) activation were inhibited by luteolin ... Moreover, IL-1beta induced activator protein-1 (AP-1) and nuclear factor-kappaB (NF-kappaB) activation were inhibited by luteolin
Ho et al., Food Chem Toxicol 2010 (Adenocarcinoma...) : It is possible that the suppressive effect of GA on MMP-2/9 might involve the inhibition of NF-kappaB activity ... It is possible that the suppressive effect of GA on MMP-2/9 might involve the inhibition of NF-kappaB activity
Wang et al., Toxicol Lett 2010 (Osteoarthritis, Knee) : In this study, we investigated the effects of E2 and BPA on nitric oxide ( NO ) production, NF-kappaB activation, and MMP-1 expression in chondrosarcoma SW1353 cells and primary human osteoarthritic chondrocytes ... In primary chondrocytes, modest concentrations of E2 reduced interleukin (IL)-1beta dependent NF-kappaB activation and MMP-1 expression ... In primary chondrocytes, modest concentrations of E2 reduced interleukin (IL)-1beta dependent NF-kappaB activation and MMP-1 expression
Wang et al., Microb Pathog 2010 : Because expressions of anti-apoptotic Bcl-2, survivin, inhibition of apoptosis protein ( cIAP ), and X-chromosome linked inhibitor of apoptosis protein ( XIAP ) are regulated by NF-kappaB , S. aureus decreased the levels of these proteins in U937 cells through inhibition of NF-kappaB ... Because expressions of anti-apoptotic Bcl-2 , survivin, inhibition of apoptosis protein ( cIAP ), and X-chromosome linked inhibitor of apoptosis protein ( XIAP ) are regulated by NF-kappaB , S. aureus decreased the levels of these proteins in U937 cells through inhibition of NF-kappaB ... Collectively, these data define a pathway that infection of primary monocytes and U937 cells with S. aureus induces a caspase dependent inhibition of NF-kappaB
Kim et al., Cell Signal 2010 : In this study, we show that KEAP1 is a new IKK binding partner, which is responsible for the down-regulation of TNFalpha stimulated NF-kappaB activation ... Reduction of KEAP1 expression by small interfering RNA enhanced NF-kappaB activity, and up-regulated the expression of NF-kappaB target genes ... Our data demonstrate that KEAP1 is involved in the negative regulation of NF-kappaB signaling through the inhibition of IKKbeta phosphorylation and the mediation of autophagy dependent IKKbeta degradation
Meneghini et al., Mol Cell Neurosci 2010 : NF-kappaB nuclear translocation occurred upon RAGE activation in SVZ derived neurospheres and its blockade ( by SN-50 ) or its absence ( in p50 ( -/- ) derived NPC ) resulted in the inhibition of the ligand mediated effects on neuronal differentiation
Duan et al., Int Immunopharmacol 2010 (Acute Lung Injury...) : Pretreatment with MDMP dampened LPS induced inflammatory responses in RAW264.7 cells, including down-regulation of TLR4-MD-2 complex on the cell surface, suppression of LPS binding to the cells, inhibition of mitogen activated protein kinase ( MAPKs ) and nuclear factor kappa B (NF-kappaB) activation , reduction of tumor necrosis factor-alpha (TNF-alpha) production
Park et al., Int Immunopharmacol 2010 (Shock, Septic) : To further elucidate the mechanism responsible for the inhibition of production of pro-inflammatory mediators by cilostazol, we examined the effect of cilostazol on LPS stimulated nuclear factor-kappaB (NF-kappaB) activation and phosphorylation of mitogen activated protein kinases ( MAPK )
Jung et al., Int Immunopharmacol 2010 : Inhibition of these inflammatory mediators appears to be at the transcriptional level, since diosgenin decreased LPS/IFN-gamma induced NF-kappaB and AP-1 activity ... Inhibition of these inflammatory mediators appears to be at the transcriptional level, since diosgenin decreased LPS/IFN-gamma induced NF-kappaB and AP-1 activity
Bae et al., Int Immunopharmacol 2010 : To assess the effects of sauchinone on LPS induced macrophages activation, we measured the production of tumor necrosis factor (TNF)-alpha and macrophage inflammatory protein (MIP)-2, and activation of mitogen activated protein kinases ( MAPKs ) including extracellular signal regulated kinase ( ERK ) 1/2, c-Jun amino terminal kinases and p38 mitogen activated protein kinase , and NF-kappaB activation in RAW264.7 cells
Markovina et al., Molecular cancer 2010 (Multiple Myeloma) : Among the multiple factors evaluated, interleukin-8 was secreted by BMSCs from MM patients at significantly higher levels compared to those from non-MM sources, and we found that IL-8 contributes to BMSC induced NF-kappaB activity
McAllister-Lucas et al., J Biol Chem 2010 (Atherosclerosis...) : Emerging evidence suggests that CARMA3 can substitute for CARMA1 in non-immune cells to assemble a CARMA3-Bcl10-MALT1 signalosome and mediate G protein coupled receptor activation of NF-kappaB ... Emerging evidence suggests that CARMA3 can substitute for CARMA1 in non-immune cells to assemble a CARMA3-Bcl10-MALT1 signalosome and mediate G protein coupled receptor activation of NF-kappaB
Stein et al., Aging 2010 (Atherosclerosis...) : However, SIRT1 prevented endothelial superoxide production, inhibited NF-kappaB signaling, and diminished expression of adhesion molecules ... However, SIRT1 prevented endothelial superoxide production, inhibited NF-kappaB signaling, and diminished expression of adhesion molecules
Kim et al., Int Immunopharmacol 2010 (Inflammation...) : DMC prevented nuclear translocation of the nuclear factor-kappaB (NF-kappaB) p65 subunit by reducing inhibitor of kappaB alpha ( I-kappaB alpha ) phosphorylation and degradation, which resulted in a suppression of NF-kappaB activities for its target genes
Zerfaoui et al., J Immunol 2010 : However, the mechanism by which PARP-1 affects NF-kappaB activation has been elusive ... PARP-1 was dispensable for LPS induced IkappaBalpha phosphorylation and subsequent degradation but was required for p65 NF-kappaB phosphorylation ... In this study, we show that PARP-1 inhibition by gene knockout, knockdown, or pharmacologic blockade prevented p65 NF-kappaB nuclear translocation in smooth muscle cells upon TLR4 stimulation, NF-kappaB DNA binding activity, and subsequent inducible NO synthase and ICAM-1 expression ... Crm1 inhibition promoted p65 NF-kappaB nuclear accumulation as well as reversed LPS induced p65 NF-kappaB phosphorylation and inducible NO synthase and ICAM-1 expression ... Pharmacologic inhibition of PARP-1 increased p65 NF-kappaB-Crm1 interaction in LPS treated smooth muscle cells
Kanoh et al., Clin Microbiol Rev 2010 : These immunomodulatory activities appear to be polymodal, but evidence suggests that many of these effects are due to inhibition of extracellular signal regulated kinase 1/2 ( ERK1/2 ) phosphorylation and nuclear factor kappa B (NF-kappaB) activation
Carlsen et al., Am J Physiol Regul Integr Comp Physiol 2010 (Ureteral Obstruction) : Pharmacological inhibition indicating that pressure induced COX-2 expression is dependent on p38 MAPK and biochemical knockdown experiments showed that NF-kappaB might be involved in the COX-2 induction by pressure
Scheper et al., PloS one 2010 : In mammalian cells, they inhibit lysine 63-type polyubiquitylation of PCNA, inhibit activation of NF-kappaB by TNF-alpha and sensitize tumor cells to chemotherapeutic agents ... Several pathways that control cell survival under stress, namely RNF8 dependent DNA damage recognition and repair, PCNA dependent DNA damage tolerance and activation of NF-kappaB by extrinsic signals, are regulated by the tagging of key proteins with lysine 63-based polyubiquitylated chains, catalyzed by the conserved ubiquitin conjugating heterodimeric enzyme Ubc13-Uev
Hostager et al., PloS one 2010 : HOIP is a ubiquitin ligase capable of mediating NF-kappaB activation through the ubiquitin dependent activation of IKKgamma ... We found that a mutant HOIP molecule engineered to lack ubiquitin ligase activity inhibited the CD40 mediated activation of NF-kappaB
Parrondo et al., Molecular cancer 2010 (Prostatic Neoplasms) : In castration-resistant DU145 and PC3 cells, Doc and 2ME2 had little effect on the high basal NF-kappaB activity and addition of parthenolide did not enhance cell death
Kim et al., Chem Biol Interact 2010 : We demonstrated apoptosis, nuclear factor-kappaB (NF-kappaB) activation and inducible nitric oxide synthase (iNOS) induction in cultured pericytes treated with MGO and MGO injected retinal vessels
Hseu et al., Food Chem Toxicol 2010 : In an earlier study, we found that Antrodia camphorata inhibited the production of lipopolysaccharide (LPS) induced cytokines, inducible nitric oxide synthase , and cyclooxygenase-2 by blocking nuclear factor-kappaB (NF-kappaB) activation in cultured RAW 264.7 macrophages ... In an earlier study, we found that Antrodia camphorata inhibited the production of lipopolysaccharide (LPS) induced cytokines, inducible nitric oxide synthase, and cyclooxygenase-2 by blocking nuclear factor-kappaB (NF-kappaB) activation in cultured RAW 264.7 macrophages ... This study was aimed at evaluating the inhibitory effects of the fermented culture broth of A. camphorata in terms of LPS induced NF-kappaB activation in transgenic mice by using a non-invasive, real-time NF-kappaB bioluminescence imaging technique ... Ex vivo imaging showed that A. camphorata suppressed LPS induced NF-kappaB activity in the small intestine, mesenteric lymph nodes, liver, spleen, and kidney
Chung et al., J Ethnopharmacol 2010 (Acute Disease...) : The activation of NF-kappaB by LPS was also alleviated by GCSB-5, which correlated with its inhibitory effect on IkB degradation
Meads et al., J Immunol 2010 : We demonstrate that NF-kappaB activation by beta ( c ) is dependent on TNFR associated factor 6 (TRAF6) and that association of TRAF6 with beta ( c ) requires a consensus binding motif found in other molecules known to interact with TRAF6
Teo et al., Nat Cell Biol 2010 : NF-kappaB is induced by ectopic expression of Rap1 , whereas its activity is inhibited by Rap1 depletion ... Furthermore, levels of Rap1 are positively regulated by NF-kappaB , and human breast cancers with NF-kappaB hyperactivity show elevated levels of cytoplasmic Rap1
Pyo et al., J Neurosci Res 2010 (Neuroblastoma) : Suppression of NF-kappaB activation using transfection of the superrepressive mutant form of IkappaBalpha ( mIkappaBalpha ) inhibited nuclear RelA expression in both types of SOD1 cells, which increased glutamate excitotoxicity in wtSOD1 cells but not in mtSOD1 cells
Gurgui et al., Cell Signal 2010 : Although our experimental data clearly show the mediatory role of sphingosine kinase ( SK ) -derived S1P in the TNF-alpha and the LPS induced activation of NF-kB , exogenously added S1P failed to trigger this transcription factor ... Although our experimental data clearly show the mediatory role of sphingosine kinase ( SK ) -derived S1P in the TNF-alpha and the LPS induced activation of NF-kB , exogenously added S1P failed to trigger this transcription factor
Carmona et al., Respir Res 2010 (Calcium Signaling) : Our data provide evidence that PCBG induces phosphorylation of the MAPKs, ERK, and p38, the activation of NF-kappaB and the subsequently secretion of IL-8 in a calcium dependent manner
Niida et al., Mol Immunol 2010 : This NF-kappaB signaling is mainly induced by the protein kinase IKK beta ... But the active IKK beta is conjugated with a monoubiquitin by the E3 ubiquitin ligase Ro52, and the IKK beta induced NF-kappaB signaling is downregulated ... Furthermore, using reporter assays, we show that Ro52 suppresses IKK beta induced NF-kappaB signaling and that this suppression is blocked by an autophagy inhibitor
Li et al., Am J Nephrol 2010 (Diabetes Mellitus, Experimental...) : The expression of TLR2 mRNA and protein level was significantly upregulated in the kidneys of diabetic rats ( p < 0.01 ), which was associated with increased renal expression of MyD88 and MCP-1 , activation of NF-kappaB and infiltration of macrophages ... The expression of TLR2 mRNA and protein level was significantly upregulated in the kidneys of diabetic rats ( p < 0.01 ), which was associated with increased renal expression of MyD88 and MCP-1, activation of NF-kappaB and infiltration of macrophages
Akhtar et al., J Immunol 2010 (AIDS Dementia Complex...) : In vitro, the HIV-1 regulatory protein transactivator of transcription induces SOCS3 in human and murine macrophages in a NF-kappaB dependent manner
Blackburn et al., Biochem J 2010 : We demonstrate that such compounds show low nanomolar IC50 values for the human 20S beta5 site in vitro, and that pharmacological inhibition of this site in cells is sufficient to potently inhibit the degradation of a tetra-ubiquitin-luciferase reporter, activation of NFkappaB ( nuclear factor kappaB ) in response to TNF-alpha ( tumour necrosis factor-alpha ) and the proliferation of cancer cells
Hong et al., Shanghai Kou Qiang Yi Xue 2010 : NF-kappaB and COX-2 expressions in the poorly differentiated and lymph node metastasis TSCC cases are higher than those in moderate- and well differentiated and non- lymph node metastasis cases, NF-kappaB and COX-2 may be involved in the carcinogenesis and development of TSCC
Zhang et al., Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases 2010 (Neuroblastoma) : However, Nec-1 could reduce Caspase-8 activity significantly ( P < 0.05, P < 0.01 ) and increase NF-kappa B protein expression ( P < 0.05, P < 0.01 ) and finally decrease Caspase-3 activity ( P < 0.05 )
Yeh et al., J Surg Res 2010 (Myocardial Reperfusion Injury) : Exogenous bradykinin administration was associated with the myocardial apoptotic response by inhibition of NF-kappaB translocation, inflammatory cytokine production, Akt activation , and elevation of the Bcl-2/Bax ratio via a NO-mediated pathway
Balasubramani et al., Immunity 2010 : STAT4 and NF-kappaB therefore cooperate at multiple cis elements to enable NF-kappaB dependent enhancement of Ifng expression
Gupta et al., J Immunol 2010 (Bone Resorption) : To investigate the mechanism of IL-3 action, we analyzed the effect of IL-3 on the receptor activator of NF-kappaB and c-Fms receptors and c-Fos, PU.1, NFAT cytoplasmic 1, and RelB transcription factors essential for osteoclastogenesis
Hunter et al., PloS one 2010 (Inflammation) : CFTR channel activity may be necessary for this suppression because the CFTR specific inhibitor CFTR ( inh172 ) significantly stimulates NF-kappaB activity by approximately 30 % in CFTR expressing 16HBE14o- cells whereas pharmacological elevation of cyclic-AMP depresses activity by approximately 25 % below baseline
Kwon et al., J Endod 2010 : However, TGFbeta1 did not affect Akt/NF-kappaB signaling to regulate the migration of MDPC-23 cells
Le Dréau et al., Glia 2010 : Using a panel of kinase inhibitors, we demonstrated that NOV action on CCL2 and CXCL1 production involved a Rho/ROCK/JNK/NF-kappaB and a Rho/qROCK/p38/NF-kappaB pathway, respectively ... Using a panel of kinase inhibitors, we demonstrated that NOV action on CCL2 and CXCL1 production involved a Rho/ROCK/JNK/NF-kappaB and a Rho/qROCK/p38/NF-kappaB pathway, respectively
Cao et al., J Neurosci Res 2010 (Gliosis) : It is concluded that Notch-1 signaling can trans-activate NF-kappaB/p65 by amplifying NF-kappaB/p65 dependent proinflammatory functions in activated microglia
Wu et al., Biochem Cell Biol 2010 (Carcinoma, Hepatocellular...) : Inhibition of NF-kappaB by PDTC decreased NF-kappaB p65 expression, enhanced cell apoptosis ratio, and increased caspase-3 activity
Huang et al., Zhonghua Xin Xue Guan Bing Za Zhi 2010 : NF-kappaB was also activated in these cells by visfatin and these effects could be significantly attenuated by Bay11-7082
Schreck et al., EMBO J 1991 : N-acetyl-L-cysteine (NAC) , a well characterized antioxidant which counteracts the effects of reactive oxygen intermediates ( ROI ) in living cells, prevented the activation of NF-kappa B by H2O2 ... NAC and other thiol compounds also blocked the activation of NF-kappa B by cycloheximide, double stranded RNA, calcium ionophore, TNF-alpha, active phorbol ester, interleukin-1 , lipopolysaccharide and lectin ... NAC and other thiol compounds also blocked the activation of NF-kappa B by cycloheximide, double stranded RNA, calcium ionophore, TNF-alpha , active phorbol ester, interleukin-1, lipopolysaccharide and lectin
Kleiner-Hancock et al., BMC cancer 2010 (Carcinoma, Squamous Cell...) : We first determined the effects of orally administered ACA ( 100 mg/kg bw ) and AUR ( 200 mg/kg bw ) on lipopolysaccharide (LPS) induced NF-kappaB activation in NF-kappaB-RE-luc ( Oslo ) luciferase reporter mice ... Both ACA and AUR suppressed LPS induced NF-kappaB activation in the report mice
Kim et al., Biochem Biophys Res Commun 2010 (Neoplasms) : TGase 2 can activate NF-kappaB through the polymerization mediated depletion of I-kappaBalpha without IKK activation
Balstad et al., Nutr Cancer 2010 : We investigated the effects of diets supplemented with apple, blackcurrant, or cherries on lipopolysaccharide (LPS) induced NF-kappaB activation in transgenic NF-kappaB-luciferase mice
Gu et al., Int J Mol Med 2010 : On the contrary, deoxyschisandrin inhibited H2O2 stimulated degradation of IkappaBalpha and activation of NF-kappaB by blocking translocation of NF-kappaB to the nucleus
Wu et al., Oncol Rep 2010 (Lung Neoplasms...) : The activated NF-kappaB not only promoted the proliferation of B16 cells, but also enhanced apoptosis resistance of B16 cells by up-regulating Bcl-2 and Bcl-xL and down regulating Bax
Park et al., Phytother Res 2010 : In addition, GT24 inhibited LPS induced activation of nuclear factor (NF)-kappaB as indicated by inhibition of degradation of I-kappaBalpha, nuclear translocation of NF-kappaB, and NF-kappaB dependent gene reporter assay
Lirdprapamongkol et al., In Vivo 2010 (Neoplasms...) : Vanillin pretreatment inhibited TRAIL induced phosphorylation of p65 and transcriptional activity of NF-kappaB
Mut et al., Turk Neurosurg 2010 (Glioblastoma) : The activation of NF-kappaB by PKC alpha and PKC delta was assessed by Western blotting after the stimulation with Phorbol 12- Myristate 13-Acetate ( PMA )
Cheng et al., Cancer Res 2010 (Neoplasms, Multiple Primary) : We further found that ZNF382 inhibited NF-kappaB and AP-1 signaling and downregulated the expression of multiple oncogenes including MYC, MITF, HMGA2, and CDK6, as well as the NF-kappaB upstream factors STAT3, STAT5B, ID1, and IKBKE, most likely through heterochromatin silencing
Fingas et al., Hepatology 2010 (Bile Duct Neoplasms...) : Because cIAP-1 and cIAP-2 also promote nuclear factor kappa B (NF-kappaB) activation by the canonical pathway, the effect of JP1584 on this signaling pathway was examined ... Because cIAP-1 and cIAP-2 also promote nuclear factor kappa B (NF-kappaB) activation by the canonical pathway, the effect of JP1584 on this signaling pathway was examined ... Treatment with JP1584 inhibited TRAIL induced NF-kappaB activation as well as TRAIL mediated up-regulation of the NF-kappaB target gene, matrix metalloproteinase 7 (MMP7)
Huang et al., Sheng Wu Gong Cheng Xue Bao 2010 (Neurodegenerative Diseases) : It has been reported that Nanog can suppress the expression of NF-kappaB
Gong et al., Biol Pharm Bull 2010 (Pancreatic Neoplasms) : Meanwhile, the TNfnA-D/ANXA2 interaction increased the phosphorylation of phosphatidylinositol 3-kinase (PI3K), Akt, inhibitory kappaB (IkappaB) kinase alpha/beta ( IKKalpha/beta ), IkappaBalpha, and p65 nuclear factor-kappaB (NF-kappaB) significantly ... Activation of p65 NF-kappaB was dependent on the phosphorylation of PI3K/Akt ... Activation of p65 NF-kappaB was dependent on the phosphorylation of PI3K/Akt
Xie et al., Ann Clin Lab Sci 2010 (Endotoxemia) : The infusion of HES130/0.4 in endotoxemic rats, especially 15 ml/kg, significantly reduced the release of plasma TNF-alpha and IL-1beta, which was consistent with the observed inhibitory effects of HES130/0.4 on NF-kappaB activation, TLR4 mRNA expression, and TLR4 protein level in monocytes
Schroecksnadel et al., Biochem Biophys Res Commun 2010 : LPS induced NF-kappaB expression in THP-1Blue cells correlates with neopterin production and activity of indoleamine 2,3-dioxygenase
Rehman et al., Int J Oral Sci 2009 (Carcinoma, Squamous Cell...) : To determine how SDF-1 alpha/CXCR4 activates nuclear factor-kappa B (NF-kappaB) and promotes oral squamous cell carcinoma ( OSCC ) invasion ... We show that the activation of NF-kappaB by CXCR4 occurs through the Carma3/Bcl10/Malt1 (CBM) complex in OSCC ... The CBM complex plays a critical role in CXCR4 induced NF-kappaB activation in OSCC
Ghosh et al., J Immunol 2010 : We have previously shown that increased nuclear accumulation of IkappaBalpha inhibits NF-kappaB activity and induces apoptosis in human leukocytes
Peng et al., Proc Natl Acad Sci U S A 2010 (Disease Models, Animal...) : Feedback regulation of transcription factor NF-kappaB by its inhibitor IkappaBalpha plays an essential role in control of NF-kappaB activity ... Here, we report that these mutant mice are defective in NF-kappaB induced expression of IkappaBalpha ... This defective feedback regulation of NF-kappaB by IkappaBalpha not only altered activity of NF-kappaB, but also the expression of NF-kappaB regulated genes ... To understand the biological significance of IkappaBalpha mediated feedback regulation of NF-kappaB , we generated mice harboring mutated kappaB enhancers in the promoter of the IkappaBalpha gene ( IkappaBalpha ( M/M ) ) to inhibit NF-kappaB regulated IkappaBalpha expression ... These findings therefore demonstrate that the IkappaBalpha mediated feedback regulation of NF-kappaB has an essential role in controlling T-cell development and functions, provide mechanistic insight into the development of Sjögren 's Syndrome, and suggest the potential of NF-kappaB signaling as a therapeutic target for Sjögren 's Syndrome and other autoimmune diseases
Liu et al., J Immunol 2010 : Overexpression of zebrafish Tirap in HEK293T cells did not activate NF-kappaB and IFN-beta, but slightly activated NF-kappaB in carp leukocyte cells ... Zebrafish Myd88 alone strongly induced the activation of NF-kappaB and IFN-beta both in HEK293T and carp leukocyte cells
Terry Powers et al., Biochemistry 2010 : TNF receptor ubiquitous scaffolding and signaling protein (TRUSS), a 90.1 kDa TNF-R1 associated scaffolding protein, also interacts with TRAF2 and IKK and contributes to TNF-alpha induced nuclear factor-kappaB (NF-kappaB) and c-Jun-NH ( 2 ) -terminal kinase ( JNK ) activation ... TNF receptor ubiquitous scaffolding and signaling protein (TRUSS) , a 90.1 kDa TNF-R1 associated scaffolding protein, also interacts with TRAF2 and IKK and contributes to TNF-alpha induced nuclear factor-kappaB (NF-kappaB) and c-Jun-NH ( 2 ) -terminal kinase ( JNK ) activation
Rohwer et al., PloS one 2010 (Stomach Neoplasms) : Furthermore, loss of HIF-1alpha abrogated, in a p53 dependent manner, chemotherapy induced DNA binding of NF-kappaB and expression of anti-apoptotic NF-kappaB target genes
Ramdhan et al., Environ Health Perspect 2010 (Body Weight...) : NFkappaB-p52 is a candidate molecular marker for inflammation caused by TRI, and PPARalpha may be involved in TRI induced hepatosteatosis
Li et al., Nat Immunol 2010 : Because of low expression of the transcription factor RelB in untreated macrophages, high p52 expression repressed basal transcription of both canonical and noncanonical NF-kappaB target genes
Lu et al., Journal of neuroinflammation 2010 (Inflammation) : Resveratrol inhibited LPS induced NF-kappaB activation in both cell types, but inhibited AP-1 activation only in microglia ... Resveratrol inhibited LPS induced NF-kappaB activation in both cell types, but inhibited AP-1 activation only in microglia
Branski et al., Laryngoscope 2010 : IL-1 beta increased nuclear factor (NF)-kappaB activation and nuclear translocation
Wang et al., J Neurosci 2010 : We found that downregulating the expression of RelA/p65 subunit of NF-kappaB or inhibiting NF-kappaB activity by overexpression of mutated form of IkappaB ( inhibitor kappaB ), which is resistant to proteolytic degradation and thus constitutively keeps NF-kappaB inactive in the cytoplasma, impeded the formation of AChR clusters in cultured C2C12 muscle cells stimulated by Agrin ... We found that downregulating the expression of RelA/p65 subunit of NF-kappaB or inhibiting NF-kappaB activity by overexpression of mutated form of IkappaB ( inhibitor kappaB ), which is resistant to proteolytic degradation and thus constitutively keeps NF-kappaB inactive in the cytoplasma, impeded the formation of AChR clusters in cultured C2C12 muscle cells stimulated by Agrin
Fujimoto et al., BMC cancer 2010 (Neoplasm Invasiveness...) : We have shown that activation of NF-kappaB and EGFR phosphorylation initially were triggered by the activation of PAR1 with alpha-thrombin
Wang et al., Zhonghua Shao Shang Za Zhi 2010 (Acute Lung Injury) : To explore the role of angiotensin II type 1 (AT1) receptor in activation of nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) in lung of mice with LPS induced acute lung injury ( ALI )
Zhang et al., Zhonghua Shao Shang Za Zhi 2010 (Burns) : To study the inhibitory effects of insulin on nuclear factor-kappa B (NF-kappaB) nuclear translocation of vascular endothelial cells induced by burn serum and its correlative mechanism
Arimoto et al., Proc Natl Acad Sci U S A 2010 : Virus induced IRF3 and NF-kappaB activation, as well as K27 linked NEMO polyubiquitination, were abrogated in TRIM23 knockdown cells, whereas TRIM23 knockdown had no effect on TNFalpha mediated NF-kappaB activation
Song et al., J Pharmacol Sci 2010 (MAP Kinase Signaling System) : Overall, our results indicated that downregulation of ICAM-1 expression by ruscogenin in HUVECs might be mediated by nuclear factor-kappaB (NF-kappaB) , but not by mitogen activated protein kinase ( MAPK ) and Akt signaling pathways
Xu et al., J Virol 2010 : NFX1 plays a role in human papillomavirus type 16 E6 activation of NFkappaB activity
Tsuchiya et al., Mol Cell 2010 : Thus, nuclear IKKbeta acts as an adaptor protein for IkappaBalpha degradation in UV-induced NF-kappaB activation
Manley et al., Prog Mol Biol Transl Sci 2010 : Transcription factors have been identified for each of these stages : a Hoxa3 dependent cascade at initial fate specification, Foxn1 for early ( and later ) TEC differentiation, and NF-kappaB for mTEC differentiation
Ropelle et al., PLoS Biol 2010 (Hyperphagia...) : This molecular mechanism, mediated by physical activity, involves the anti-inflammatory protein IL-10 , a core inhibitor of IKKbeta/NF-kappaB signaling and ER stress
Furukawa et al., Oncol Rep 2010 (Disease Models, Animal...) : In vitro, nafamostat mesilate inhibited NF-kappaB activation of human pancreatic cancer cell line ( Panc-1 ) by suppressing IkappaBalpha phosphorylation and induced caspase-8 mediated apoptosis ... Assays of primary tumors also indicated that nafamostat mesilate inhibited NF-kappaB activation by suppressing IkappaBalpha phosphorylation, resulting in caspase-8 mediated apoptosis
Majewski et al., J Biol Chem 2010 (Colitis...) : Cooperative role of NF-{kappa}B and poly(ADP-ribose) polymerase 1 ( PARP-1 ) in the TNF induced inhibition of PHEX expression in osteoblasts
Uto et al., Am J Chin Med 2010 : Furthermore, EFE significantly inhibited LPS induced NF-kappaB binding activity, which was associated with the inhibition of IkappaB-alpha degradation ... Furthermore, EFE significantly inhibited LPS induced NF-kappaB binding activity, which was associated with the inhibition of IkappaB-alpha degradation
Simko et al., J Hypertens 2010 (Hypertension) : Inhibition of nuclear factor kappa B (NF-kappaB) potentiates hypertension development, enhances oxidative load, increases the cross-sectional area of the aorta and reduces nitric oxide ( NO ) synthase activity in L-NAME hypertension
Taniguchi et al., Reproduction 2010 : PGF ( 2a ) increased NFKB p65 protein expression in the nucleus of luteal cells 30 min after PGF ( 2a ) injection, and electrophoretic mobility shift assay revealed that PGF ( 2a ) increased binding activities of NFKB to the NFKB consensus sequence of the Ptgs2 gene promoter ... Simultaneous administration of both superoxide dismutase and catalase to scavenge reactive oxygen species ( ROS ) inhibited the increases of nuclear NFKB p65 protein expression, lipid peroxide levels, and Ptgs2 mRNA expression induced by PGF ( 2a )
Willems et al., Molecular cancer 2010 (Liposarcoma, Myxoid) : Kinome profiling of myxoid liposarcoma reveals NF-kappaB-pathway kinase activity and casein kinase II inhibition as a potential treatment option
Madge et al., J Biol Chem 2010 : Classical NF-kappaB activation negatively regulates noncanonical NF-kappaB dependent CXCL12 expression
Cui et al., J Biol Chem 2010 (Alzheimer Disease...) : Differential regulation of interleukin-1 receptor associated kinase-1 ( IRAK-1 ) and IRAK-2 by microRNA-146a and NF-kappaB in stressed human astroglial cells and in Alzheimer disease ... Differential regulation of interleukin-1 receptor associated kinase-1 ( IRAK-1 ) and IRAK-2 by microRNA-146a and NF-kappaB in stressed human astroglial cells and in Alzheimer disease
Shimada et al., Oncol Res 2010 : Even after mouse monocytic leukaemia RAW264.7 cells had been washed free of ( - ) -DHMEQ, lipopolysacharide (LPS) induced activation of NF-kappaB in these cells was still inhibited ... When it was topically added to RAW264.7 cells in which NF-kappaB was activated by LPS , the inhibition lasted at least for 2 h. NF-kappaB derectly upregulates expression of iNOS that produces NO
Lindholm et al., New Biol 1990 (Cell Transformation, Viral) : Tax1 activation of NF-kappa B was not sensitive to either staurosporin or prolonged stimulation with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate, suggesting that Tax1 dependent NF-kappa B activation did not require the protein kinase C pathway ... Purified Tax1 did not directly increase NF-kappa B binding activity in 70Z/3 cytoplasmic extracts, suggesting that NF-kappa B induction may require cellular factors
Tareen et al., Virology 2011 : We show that human Trim5a also affects levels of TAB2, resulting in abrogation of TAB2 dependent NF-kappaB activation
Messer et al., Cytokine 1990 : The high homology of the well characterized H-2Kb enhancer sequence with the TNF-beta site with regard to sequence and factor binding strongly supports a physiological role for NF-kappa B in the inducible expression of the TNF-beta gene ... Our observation that the TNF-beta protein can rapidly induce the DNA binding activity of NF-kappa B in Jurkat T cells and transiently increase TNF-beta mRNA levels suggests that NF-kappa B can mediate a positive autoregulation of TNF-beta synthesis
Shakhov et al., J Exp Med 1990 : We speculate that NF-kappa B and/or related proteins are involved in the LPS induced transcriptional activation of the TNF-alpha gene, and that factors interacting with the Y box can additionally modulate the activity of the gene in macrophages
Rooney et al., Immunogenetics 1990 (Lymphoma) : Slow response variant of the B lymphoma 70Z/3 defective in LPS activation of NF-kappa B ... Although NF-kappa B can not be activated by LPS , it can be activated by treatment with phorbol ester ( PMA )
Singh et al., J Biol Chem 2011 (Disease Models, Animal...) : The apolipoprotein E-mimetic peptide COG112 inhibits NF-kappaB signaling, proinflammatory cytokine expression, and disease activity in murine models of colitis
Liu et al., Mol Biol Rep 2011 (Genetic Predisposition to Disease...) : Virus induced signaling adapter (VISA) , an important adaptor protein linking both RIG-I and MDA-5 to downstream signaling events, may mediates the activation of NF kappaB and IRFs and the induction of type I IFN
Pessara et al., Mol Cell Biol 1990 : Comparison of the TNF-alpha induced factor and lipopolysaccharide induced NF-kappa B in gel mobility shift assays upon partial protease digestion suggests similar DNA binding protein cores
Bottero et al., J Virol 2011 (Herpesviridae Infections) : Phosphorylation and polyubiquitination of transforming growth factor beta activated kinase 1 are necessary for activation of NF-kappaB by the Kaposi 's sarcoma associated herpesvirus G protein coupled receptor
Wang et al., Cancer Res 2011 (Prostatic Intraepithelial Neoplasia...) : Activation of NF-{kappa}B by TMPRSS2/ERG Fusion Isoforms through Toll-Like Receptor-4
Rogler et al., J Crohns Colitis 2007 : Taken together these data indicate that NOD2 mediated NF-kappaB activation, subsequent induction of anti-microbial peptides such as defensins and induction of cytokine expression are essential for the function of the intestinal barrier and for the prevention of bacterial translocation
Zhao et al., Zhongguo Ying Yong Sheng Li Xue Za Zhi 2006 (Shock, Septic) : The role of activated nuclear factor-kappa B(NF-kappaB) on the expression of heme oxygenase-1 induced by lipopolysaccharide (LPS) in rats ' myocardium was obversed, and the effect exerted in endotoxic shock was explored
Park et al., Front Biosci (Elite Ed) 2011 (Inflammation) : TNF-alpha mediated NF-kappaB activation is constantly extended by transglutaminase 2 ... In the TGase 2-inducible EcR23/TG cell line, TGase 2 over-expression resulted in sustained activation of NF-kappa B in the presence of TNF-alpha , for up to 24 hrs, while in the absence of TGase 2 induction, NF-kappaB activity was restored to basal levels within 6 hrs of TNF-alpha treatment
Deeb et al., Front Biosci (Elite Ed) 2011 : DNA binding assays revealed that combination treatment suppressed both basal and TNF-alpha induced NF-kappaB activation
Wang et al., Front Biosci (Elite Ed) 2011 (Brain Ischemia) : APC with sevofurane markedly improved neurological performance of stroke rats, significantly decreased infarct volume, and robustly suppressed activation of NF-kappa B and p38 MAPK, and expression of inflammatory cytokines
Drafahl et al., PloS one 2010 (Inflammation) : The receptor tyrosine kinase FGFR4 negatively regulates NF-kappaB signaling
Urban et al., Genes Dev 1990 : Furthermore, excess p65 could very efficiently activate the latent form of NF-kappa B composed of p50, p65, and I kappa B, presumably by binding the I kappa B, which was released from the complex due to its inherent off rate
Kissner et al., PloS one 2011 (Inflammation) : Here we report that human monocytes treated with SEA, SEB, or anti-MHC class II monoclonal antibodies up regulated MyD88 expression, induced activation of NF-kB , and increased expression of IL-1R1 accessory protein, TNF-a and IL-1ß ... Here we report that human monocytes treated with SEA, SEB, or anti-MHC class II monoclonal antibodies up regulated MyD88 expression, induced activation of NF-kB , and increased expression of IL-1R1 accessory protein, TNF-a and IL-1ß ... Here we report that human monocytes treated with SEA, SEB, or anti-MHC class II monoclonal antibodies up regulated MyD88 expression, induced activation of NF-kB , and increased expression of IL-1R1 accessory protein, TNF-a and IL-1ß ... Here we report that human monocytes treated with SEA, SEB, or anti-MHC class II monoclonal antibodies up regulated MyD88 expression, induced activation of NF-kB , and increased expression of IL-1R1 accessory protein, TNF-a and IL-1ß
Alvarado et al., Medicina (B Aires) 2011 : The treatment of HEK293 cells with TNF-a induced an increase in the protein levels of RAC3 and this was blocked by an NF-kB specific inhibitor, suggesting that this transcription factor is involved in the cytokine effect
Di Santo et al., Journal of thrombosis and haemostasis : JTH 2011 (Thrombosis) : The results demonstrated that, in MN interacting with activated PLT : ( i ) TF activity, antigen and mRNA were low until 8-10 h and dramatically increased thereafter, up to 24 h ; ( ii ) according to the kinetics of TF expression in MN, GSK3ß undergoes phosphorylation on serine 9, a process associated with down-regulation of enzyme activity ; ( iii ) pharmacological blockade of GSK3 further increased TF expression and was accompanied by increased accumulation of NF-kB , in the nucleus ; ( iv ) blockade of phosphoinositide-3 kinase ( PI(3)K ) by wortmannin inhibited PLT induced TF expression ; and ( v ) according to the established role of the GSK3 downstream insulin receptor, insulin increased PLT induced TF expression in a PI(3)K dependent manner ... The results demonstrated that, in MN interacting with activated PLT : ( i ) TF activity, antigen and mRNA were low until 8-10 h and dramatically increased thereafter, up to 24 h ; ( ii ) according to the kinetics of TF expression in MN, GSK3ß undergoes phosphorylation on serine 9, a process associated with down-regulation of enzyme activity ; ( iii ) pharmacological blockade of GSK3 further increased TF expression and was accompanied by increased accumulation of NF-kB , in the nucleus ; ( iv ) blockade of phosphoinositide-3 kinase ( PI(3)K ) by wortmannin inhibited PLT induced TF expression ; and ( v ) according to the established role of the GSK3 downstream insulin receptor, insulin increased PLT induced TF expression in a PI(3)K dependent manner
Sønder et al., J Biol Chem 2011 : IL-17 induced NF-kappaB activation via CIKS/Act1 : physiologic significance and signaling mechanisms ... IL-17 induced NF-kappaB activation via CIKS/Act1 : physiologic significance and signaling mechanisms ... IL-17 induced NF-kappaB activation via CIKS/Act1 : physiologic significance and signaling mechanisms
Gentle et al., J Biol Chem 2011 : In TNF stimulated cells, RIPK1 promotes cell survival by stabilizing TRAF2 and cIAP1, which limits induction of non-canonical NF-kappaB and activation of caspase-8
Qiu et al., FASEB J 2011 : Thymosin beta4 inhibits TNF-alpha induced NF-kappaB activation, IL-8 expression, and the sensitizing effects by its partners PINCH-1 and ILK ... Thymosin beta4 inhibits TNF-alpha induced NF-kappaB activation , IL-8 expression, and the sensitizing effects by its partners PINCH-1 and ILK
Liu et al., Mol Cancer Res 2011 (Leukemia, Lymphocytic, Chronic, B-Cell) : STAT-3 activates NF-kappaB in chronic lymphocytic leukemia cells
Coscia et al., Leukemia 2011 (Leukemia, Lymphocytic, Chronic, B-Cell) : Nuclear factor-kB (NF-kB) was rapidly inactivated, and B-cell leukemia/lymphoma 2 (Bcl-2) expression progressively down-regulated in the UM CLL B cells
Usui et al., Nihon rinsho. Japanese journal of clinical medicine 2011 (Diabetes Mellitus, Type 2...) : These inflammatory mediators inhibit insulin signaling with several mechanisms, such as serine-phosphorylation of IRS-1, the induction of SOCS3 and the activation of JNK or NFkappaB signaling in insulin-target tissues
Damgaard et al., Discov Med 2011 (Inflammation) : Recent evidence suggests that cIAP1, cIAP2, and XIAP facilitate ubiquitin dependent signaling activated by these PRRs and mediate activation of nuclear factor-kappa B (NF-kappaB) transcription factors as well as the MAP kinases p38 and JNK
Toubiana et al., J Biol Chem 2011 : IMPDHII protein inhibits Toll-like receptor 2-mediated activation of NF-kappaB
Lee et al., The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology 2011 : Therefore, nuclear translocation of NF-kB induced by LPS stimulation was significantly suppressed with DC pretreatment
Khodagholi et al., Mol Cell Biochem 2011 (Calcium Signaling) : It also inhibited LPS induced cyclooxygenase 2 (COX-2), TNF-a, nuclear factor KappaB (NF-kB) , and caspase-3 expression in a dose dependent manner while stabilizing nuclear factor-erythroid 2 p45 related factor 2
Niranjan et al., Cell Mol Neurobiol 2011 (Astrocytoma...) : Treatment with nimesulide also attenuated LPS induced Ca ( 2+ ) ion, iNOS, NF-kB , and c-fos expressions, but does not significantly influence CHOP, c-jun protein expressions, and mRNA levels of IL-6, IL-1a, IL-1ß, and mPGES-1 genes
Knoell et al., Int Z Vitam Ernahrungsforsch Beih 2010 (Inflammation...) : Whether ZIP8 functions beyond the conventional role of a zinc transporter remains a work in progress, although new evidence has revealed that ZIP8 expression itself is regulated by NF-kappaB
Hazan et al., Proc Natl Acad Sci U S A 1990 : Stimulation of a human T-cell clone with anti-CD3 or tumor necrosis factor induces NF-kappa B translocation but not human immunodeficiency virus 1 enhancer dependent transcription ... Interleukin 2 induced proliferation but not NF-kappa B translocation or LTR transactivation ... Our finding that induction of NF-kappa B by tumor necrosis factor or antibody to CD3 is not sufficient to induce HIV enhancer dependent transcription in cloned T cells contrasts with results obtained in most lymphoblastoid T-cell lines and indicates that normal T lymphocytes differ from tumoral T cells in terms of requirements for HIV LTR activation
Balasubramanian et al., J Biol Chem 2011 : The interferon-gamma induced GTPase, mGBP-2, inhibits tumor necrosis factor alpha (TNF-alpha) induction of matrix metalloproteinase-9 (MMP-9) by inhibiting NF-kappaB and Rac protein ... The interferon-gamma induced GTPase, mGBP-2, inhibits tumor necrosis factor alpha (TNF-alpha) induction of matrix metalloproteinase-9 (MMP-9) by inhibiting NF-kappaB and Rac protein
Oussaief et al., J Virol 2011 : NF-kappaB mediated modulation of inducible nitric oxide synthase activity controls induction of the Epstein-Barr virus productive cycle by transforming growth factor beta 1
Xu et al., J Biol Chem 2011 : miR-365 , a novel negative regulator of interleukin-6 gene expression, is cooperatively regulated by Sp1 and NF-kappaB
Guo et al., Proc Natl Acad Sci U S A 2011 : FER tyrosine kinase ( FER ) overexpression mediates resistance to quinacrine through EGF dependent activation of NF-kappaB
Konecny et al., Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences 2010 : By controlling the NF-kB-RelB complex, IKKa signaling regulates the transcription of the Skp2 and correspondingly p27Kip1 ... By controlling the NF-kB-RelB complex, IKKa signaling regulates the transcription of the Skp2 and correspondingly p27Kip1
Nagaya et al., Int J Oncol 1997 : CAL-A ( 0.5 nM ) also augmented the TNF alpha induced phosphorylation of I kappa B-alpha , an inhibitor of NF-kappa B
Denko et al., Int J Oncol 1997 : Gel shift analysis and reporter gene expression indicated that TPCK blocked Ha-ras induced NF-kappa B activity, while only having minimal effects on Ha-ras induced AP-1 activity ... TPCK is therefore able to Inhibit the Ha-ras transformed phenotype of cells by inhibiting the transcriptional activity of NF-kappa B , while having little effect upon transcriptional activity of AP-1
Gewurz et al., J Virol 2011 : Canonical NF-kappaB activation is essential for Epstein-Barr virus latent membrane protein 1 TES2/CTAR2 gene regulation
Wang et al., Nucleic Acids Res 2011 : NF-kappaB P50/P65 hetero-dimer mediates differential regulation of CD166/ALCAM expression via interaction with micoRNA-9 after serum deprivation, providing evidence for a novel negative auto-regulatory loop
Ghosh et al., Nature 1990 (Second Messenger Systems) : Using gel retardation assays we found that PKC, cyclic AMP dependent protein kinase (PKA) and a haem regulated eIF-2 kinase ( HRI ) could activate NF-kappa B in vitro, whereas casein kinase II was ineffective
Charoenthongtrakul et al., J Virol 2011 : Human T cell leukemia virus type 1 Tax inhibits innate antiviral signaling via NF-kappaB dependent induction of SOCS1
Talbot et al., Peptides 2011 (Inflammation) : Data suggest that the increased expression of B1R by TPM in A549 cells is mediated by oxidative stress, IL-1ß and NF-kB but not by cyclooxygenases or TNF-a
Bomsztyk et al., J Biol Chem 1990 : Although Bt2cAMP or PGE2 when used alone had no effect, they did consistently modify the level of NF-kappa B activity induced by IL-1 alpha ... Such a regulatory role is supported by the observation that cAMP modulates the IL-1 alpha induced NF-kappa B activity
van Zoelen et al., Front Biosci (Schol Ed) 2011 (Communicable Diseases...) : Engagement of RAGE by its diverse ligands results in receptor dependent signaling and activation of NF-kappaB
Simone et al., PloS one 2011 : Lycopene inhibits NF-kB mediated IL-8 expression and changes redox and PPAR? signalling in cigarette smoke stimulated macrophages ... NF-kB controlled the transcription of IL-8 induced by CSE, since PDTC prevented such a production ... Lycopene suppressed CSE induced NF-kB DNA binding, NF-kB/p65 nuclear translocation and phosphorylation of IKKa and IkBa
Chiang et al., Genetics and molecular biology 2009 : The results showed that, with a 36 % inhibition of NF-kB 1 expression, gene expression of both Toll-like receptor (TLR) 4 and interleukin (IL)-6 was consistently and significantly increased at both 1 h and 4 h following SE challenge, whereas the gene expression of MyD88 and IL-1ß was increased at 1 h and 4 h, respectively ... The results showed that, with a 36 % inhibition of NF-kB 1 expression, gene expression of both Toll-like receptor (TLR) 4 and interleukin (IL)-6 was consistently and significantly increased at both 1 h and 4 h following SE challenge, whereas the gene expression of MyD88 and IL-1ß was increased at 1 h and 4 h, respectively
Hohmann et al., J Biol Chem 1990 : TNF-alpha and TNF-beta activate the transcription factor NF-kappa B in HL60 and HEp2 cells ... TNF-alpha and TNF-beta activate the transcription factor NF-kappa B in HL60 and HEp2 cells ... Maximal activation of NF-kappa B required binding of TNF-alpha or TNF-beta to 20-25 % of the total number of TNF receptors and was achieved within minutes after the addition of TNF-alpha to HL60 cells ... Maximal activation of NF-kappa B required binding of TNF-alpha or TNF-beta to 20-25 % of the total number of TNF receptors and was achieved within minutes after the addition of TNF-alpha to HL60 cells ... Both TNF-alpha and TNF-beta activate NF-kappa B at 5-10-fold lower concentrations in HL60 cells compared with HEp2 cells, and this correlates well with their different affinities for binding to these cells ... Both TNF-alpha and TNF-beta activate NF-kappa B at 5-10-fold lower concentrations in HL60 cells compared with HEp2 cells, and this correlates well with their different affinities for binding to these cells
Potapovich et al., Toxicol Appl Pharmacol 2011 (Dermatitis...) : They also suppressed NFkappaB activation by LPS and T/I. Verbascoside and quercetin invariably impaired EGFR phosphorylation and UV-associated aryl hydrocarbon receptor (AhR) mediated signaling, while rutin, polydatin and resveratrol did not affect EGFR phosphorylation and further activated AhR machinery in UV-exposed keratinocytes
Hohmann et al., J Biol Chem 1990 : Both the types A and B receptors mediated TNF alpha induced activation of the transcription factor NF-kappa B
Hu et al., Proc Natl Acad Sci U S A 2011 : Noncanonical NF-kappaB regulates inducible costimulator (ICOS) ligand expression and T follicular helper cell development ... Noncanonical NF-kappaB regulates inducible costimulator (ICOS) ligand expression and T follicular helper cell development
Volkov et al., Tsitologiia 2011 (Carcinoma, Hepatocellular...) : Effect of carcinogenic polycyclic aromatic hydrocarbons (PAH) benzo ( a ) pyrene ( BP ) and 3-methylcholanthrene ( MC ) on transcription factor NF-kappaB activation was studied ... The role of Ah receptor in PAH action on NF-kappaB activation is discussed
Liu et al., Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2011 : Our study reveals that NF-kappaB is activated in response to TRIM38
He et al., Mol Biol (Mosk) 2011 : Overexpression of TSR2 in human epidermal HEp-2 cells inhibited the transcriptional activity of NF-kappaB , with or without tumor necrosis factor a stimulus, and induced HEp-2 cell apoptosis
Libermann et al., Mol Cell Biol 1990 : However, stimulation of U-937 and HeLa cells by inducers of NF-kappa B led to a dramatic increase in CAT activity ... These results suggest that NF-kappa B is an important mediator for activation of the IL-6 gene by a variety of IL-6 inducers in both U-937 and HeLa cells and that alternative inducible enhancer elements contribute in a cell-specific manner to IL-6 gene induction
Akhtar et al., BMC complementary and alternative medicine 2011 (Osteoarthritis) : The inhibitory effects of HLM were mediated by inhibiting the activation of nuclear factor (NF)-kB in human OA chondrocytes in presence of IL-1ß
Iyer et al., J Immunol 2011 : Antioxidant c-FLIP inhibits Fas ligand induced NF-kappaB activation in a phosphatidylinositol 3-kinase/Akt dependent manner ... Antioxidant c-FLIP inhibits Fas ligand induced NF-kappaB activation in a phosphatidylinositol 3-kinase/Akt dependent manner ... Antioxidant c-FLIP inhibits Fas ligand induced NF-kappaB activation in a phosphatidylinositol 3-kinase/Akt dependent manner
Meichle et al., J Biol Chem 1990 (Leukemia) : NF kappa B may play a central role in mediating TNF 's gene regulatory action ; however, the molecular mechanisms of TNF induced NF kappa B activation are poorly understood ... The data indicate that PK-C is not required for NF kappa B activation by TNF and imply a novel, PK-C independent mechanism of physiological NF kappa B activation
Song et al., PloS one 2011 (Carotid Artery Injuries...) : In vitro data revealed that CD40 deficiency blocked CD40L induced NF-kB p65 nuclear translocation in leukocytes ... In vitro data revealed that CD40 deficiency blocked CD40L induced NF-kB p65 nuclear translocation in leukocytes
Ghose et al., PloS one 2011 (Huntington Disease) : Regulation of miR-146a by RelA/NFkB and p53 in STHdh ( Q111 ) /Hdh ( Q111 ) cells, a cell model of Huntington 's disease ... We conclude that ( i ) miR-125b and miR-150 target p53, which in turn regulates RelA/NFkB and miR-146a expressions ; ( ii ) reduced miR-125b and miR-150 expressions, increased p53 level and decreased RelA/NFkB and miR-146a expressions originate from mutant HTT ( iii ) p53 directly or indirectly regulates the expression of miR-146a ... We conclude that ( i ) miR-125b and miR-150 target p53, which in turn regulates RelA/NFkB and miR-146a expressions ; ( ii ) reduced miR-125b and miR-150 expressions, increased p53 level and decreased RelA/NFkB and miR-146a expressions originate from mutant HTT ( iii ) p53 directly or indirectly regulates the expression of miR-146a
Xiong et al., Genome Res 2012 (Crohn Disease...) : The analysis of two human diseases, glioblastoma and Crohn 's disease, detected abnormalities in previously identified disease associated pathways, such as pathways related to PI3K signaling, DNA damage response, and the activation of NFKB
Langone et al., Int J Cancer 2012 (Brain Neoplasms...) : Furthermore, overexpression of NF-kB in the B16F10 cells blocked adduct evoked stimulation of caspase-3/7 activity
Zuo et al., Eur J Pharmacol 2011 (Carcinoma, Hepatocellular) : Furthermore, dexamethasone inhibited LPS induced activation of NF-kappaB and expression of the hTERT in HepG ( 2 ) cells
Zhang et al., Wei Sheng Yan Jiu 2011 : To investigate the effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates ( CSC )
Hill et al., PloS one 2011 : At the mechanistic level, PBP2 induces nuclear localization of p65 NF-kB of 70.7 ± 5.1 % cells versus 12 ± 2.6 % in untreated DCs and needs TLR4 expression to mature DCs
McCarty et al., Med Hypotheses 2012 (Neoplasm Metastasis...) : The transcription of Lin28B is promoted by NF-kappaB and by Myc ; hence, practical measures which antagonize NF-kappaB or Myc activity may complement the utility of metformin for boosting let-7 expression and controlling cancer stemness ; salsalate, antioxidants, tyrosine kinase and cox-2 inhibitors, ribavirin, vitamin D, gamma-secretase inhibitors ( when available ), and parenteral curcumin may have some utility in this regard
Cappello et al., PloS one 2011 (Pulmonary Disease, Chronic Obstructive) : Hsp60 induction by NFkB-p65 and its release by epithelial cells after oxidative stress can have a role in maintaining inflammation, e.g., by stimulating neutrophils activity
Alberti et al., Oncogene 2012 (Neoplasms, Glandular and Epithelial...) : In vitro analysis of EOC cell lines revealed that ligand stimulated EGFR activated NFkB dependent transcription and induced secretion of IL-6 and plasminogen activator inhibitor ( PAI-1 )
Leychenko et al., PloS one 2011 (Cardiomegaly) : Stretch induced hypertrophy activates NFkB mediated VEGF secretion in adult cardiomyocytes ... Cyclic mechanical stretch mediated VEGF secretion was blocked by an NFkB peptide inhibitor and expression of a dominant negative mutant IkBa, but not by inhibitors of the MAPK/ERK1/2 or PI3K pathways
Shaposhnikov et al., Adv Gerontol 2011 (Inflammation) : No synergic effect between conditionally activated PARP-1 expression and NF-kB inhibition is observed
Risal et al., Pathol Int 2012 (Drug-Induced Liver Injury...) : In addition, Pin1 overexpression increased NF-kB activity, as evidenced by increased DNA binding
Strebovsky et al., Frontiers in bioscience (Landmark edition) 2012 : A model is presented that integrates the current, partly conflicting, data on the role of SOCS proteins in innate immunity 's NFkappaB signaling
Ballard et al., Cell 1990 : v-Rel , but not nontransforming v-Rel mutants, binds to the kappa B enhancer and inhibits NF-kappa B-activated transcription from the IL-2 receptor alpha promoter and HIV-1 LTR
Talbot et al., Journal of neuroinflammation 2012 : NAC ( 1 g/kg/d x 7 days ) prevented B(1)R up-regulation, superoxide anion production and NF-kB activation induced by capsaicin ( 15 mg/kg )
Karlsson et al., BMC microbiology 2012 : Thus the urogenital probiotic L. rhamnosus GR-1 modulated the activation of the NF-kappaB through increased levels of TLR4 on the bladder cells and altered subsequent release of cytokines from urothelial cells
Vandermark et al., Virology 2012 (Cell Transformation, Viral) : In contrast, activation of NF-kB by constitutive expression of p65 inhibited proliferation and immortalization
Li et al., BMC molecular biology 2012 (Esophageal Neoplasms) : Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells ... Reporter assay further showed that NF-kappaB induced miR-34a transcriptional activity was reduced by p53 impairment
Xu et al., Neoplasma 2012 (Colorectal Neoplasms) : Overexpression of Sp1 or NF-kB increased the activity of the APRIL promoter
Colombo et al., PloS one 2012 (Lupus Erythematosus, Systemic...) : The effect of LAIR1 ligation by specific monoclonal antibodies, collagen or collagen producing mesenchymal stromal cells from reactive lymph nodes or bone marrow on Ig production by pokeweed mitogen and B cell receptor (BCR) mediated NF-kB activation was assessed by ELISA and TransAM assay ... The effect of LAIR1 ligation by specific monoclonal antibodies, collagen or collagen producing mesenchymal stromal cells from reactive lymph nodes or bone marrow on Ig production by pokeweed mitogen and B cell receptor (BCR) mediated NF-kB activation was assessed by ELISA and TransAM assay
Geisert et al., Cell Tissue Res 2012 : A model for the regulation of cellular progesterone receptor expression and NFKB activation for endometrial receptivity and conceptus attachment is continuing to evolve and is discussed in the present review
Xiao et al., Zhonghua Nan Ke Xue 2012 (Prostatic Neoplasms) : All the rats were killed 2 weeks after CMTM5 injection and the tumor tissue harvested for detection of the inhibitory effect of CMTM5 on the expressions of VEGF and NF-kappaB proteins by immunohistochemistry
Fouad et al., Pak J Biol Sci 2011 (Necrosis) : The regulation of p53 expression is mediated by the transcription factor NF-kappaB
Jordan et al., Am J Hum Genet 2012 (Arthritis, Psoriatic...) : CARD14 activates nuclear factor kappa B (NF-kB) , and compared with wild-type CARD14, the p.Gly117Ser and p.Glu138Ala substitutions were shown to lead to enhanced NF-kB activation and upregulation of a subset of psoriasis associated genes in keratinocytes
Wang et al., Zhonghua Gan Zang Bing Za Zhi 2011 (Carcinoma, Hepatocellular...) : p38 MAPK inhibitor ( SB203580 ) could significantly inhibit IL-8 production in a dose dependent manners ( F = 65.47, P < 0.01 ), and partially inhibited NF-kB p65 nuclear translocation in a dose dependent manner ( F=141.20, P < 0.05 ) ... p38 MAPK inhibitor ( SB203580 ) could significantly inhibit IL-8 production in a dose dependent manners ( F = 65.47, P < 0.01 ), and partially inhibited NF-kB p65 nuclear translocation in a dose dependent manner ( F=141.20, P < 0.05 ) ... p38 MAPK inhibitor ( SB203580 ) could significantly inhibit IL-8 production in a dose dependent manners ( F = 65.47, P < 0.01 ), and partially inhibited NF-kB p65 nuclear translocation in a dose dependent manner ( F=141.20, P < 0.05 )
Hou et al., Wei Sheng Yan Jiu 2012 : The expression of NF-kappaB , MCP-1, IL-1beta, IL-6 and IL-8 in HUVECs up-regulated by TNF-alpha was promoted by CIT
Vose et al., Am J Hematol 2012 (Asymptomatic Diseases...) : At the time of relapse, agents directed at activated pathways in MCL cells such as bortezomib ( NFkB inhibitor ), BTK inhibitors or CAL-101 ( B-cell receptor inhibitors ) or lenalidamide ( antiangiogenesis ) have clinical activity in MCL patients
Van Beek et al., Cell reports 2012 (Carcinoma, Hepatocellular...) : Here, we demonstrate that the saturated FA, palmitate, requires Bcl10 for NF-kB activation in hepatocytes ... Surprisingly, while CARMA3 also participates in the palmitate response, MALT1 is completely dispensable, thereby revealing an apparent nonclassical role for Bcl10 in NF-kB signaling
Smith et al., Genes Dev 1990 : In contrast, Tax transactivation of the interleukin-2 receptor-alpha gene ( IL-2R alpha ) and LTR of the type 1 human immunodeficiency virus ( HIV-1 ) involves the induced nuclear expression of NF-kappa B
Hussain et al., PloS one 2012 (Lymphoma, Primary Effusion) : Inhibition of NFkB activity by Bay11-7085 resulted in decreased expression of p65 in the nuclear compartment as detected by EMSA assays
Finotti et al., Artif DNA PNA XNA 2012 (Cystic Fibrosis) : Since transcription factor NF-kappaB plays a critical role in IL-8 expression, the transcription factor decoy ( TFD ) strategy might be of interest
Yin et al., J Atheroscler Thromb 2012 : In addition, apoA-I inhibited the sCD40L stimulated activation of nuclear factor-kB (NF-kB)
Gromkowski et al., Int Immunol 1990 : Additionally, our data show that both dsRNA and LPS, as well as TNF-alpha itself, rapidly induce nuclear factor-kappa B (NF-kappa B) , a DNA binding protein implicated in regulation of gene expression ... Additionally, our data show that both dsRNA and LPS , as well as TNF-alpha itself, rapidly induce nuclear factor-kappa B (NF-kappa B) , a DNA binding protein implicated in regulation of gene expression
Zhu et al., Zhongguo Zhong Yao Za Zhi 2012 (Carcinoma, Non-Small-Cell Lung) : [ Effect of NF-kappaB on inhibition of non-small cell lung cancer cell cyclooxygenase-2 by brucine ]
Zhong et al., Biochem Biophys Res Commun 2012 (MAP Kinase Signaling System) : Omentin, NF-kB inhibitor ( BAY11-7082 ) and ERK inhibitor ( PD98059 ) reduced the up-regulation of ICAM-1 and VCAM-1 induced by TNF-a ... Omentin, NF-kB inhibitor ( BAY11-7082 ) and ERK inhibitor ( PD98059 ) reduced the up-regulation of ICAM-1 and VCAM-1 induced by TNF-a
Jan et al., Microbiol Immunol 2012 : Inhibition of nuclear factor (NF)-kappa B ( ?B ) by helenalin and p38 mitogen activated protein kinase ( MAPK ) by SB203580 prevented production of IL-12 p40 , IL-12 p70, and IL-10 ... Inhibition of nuclear factor (NF)-kappa B ( ?B ) by helenalin and p38 mitogen activated protein kinase ( MAPK ) by SB203580 prevented production of IL-12 p40, IL-12 p70 , and IL-10 ... Inhibition of nuclear factor (NF)-kappa B ( ?B ) by helenalin and p38 mitogen activated protein kinase ( MAPK ) by SB203580 prevented production of IL-12 p40, IL-12 p70, and IL-10
Kutsenko et al., FiziolohichnyÄ­ zhurnal (Kiev, Ukraine : 1994) 2012 : Our results indicate for the involvement of PPAR-gamma in the regulation of NF-kB activity which plays one of the most significant roles in the inflammation realization, oncogenesis, and so on
Hochstenbach et al., Cancer Epidemiol Biomarkers Prev 2012 (Micronuclei, Chromosome-Defective) : In particular, male-specific TNF-alpha-NF-kB signaling upon dioxin exposure and activation of the Wnt-pathway in boys upon acrylamide exposure might represent possible mechanistic explanations for gender specificity in the incidence of childhood leukemia
Guo et al., PloS one 2012 (Inflammation) : BME also attenuated TNFalpha induced activation of NFkappaB in differentiating preadipocytes and partially restored TNFalpha mediated suppression on adipogenesis ... Using a non-adipogenic HEK293 cell line transfected with luciferase reporter genes, we demonstrated that BME reduced basal and TNFalpha induced NFkappaB activity and increased basal and ciglitazone induced PPARgamma activity ; both may contribute to the pro-adipogenic effect of BME in differentiating F442A preadipocytes
Banerjee et al., Breast Cancer Res Treat 2012 (Breast Neoplasms...) : Moreover, previous studies have shown that Pg extracts decrease inflammation in lung cancer cell lines by inhibiting phosphatidylinositol-3,4,5-trisphosphate ( PI3K ) -dependent phosphorylation of AKT in vitro and inhibiting the activation of NF-kB in vivo
Patruno et al., Biochim Biophys Acta 2012 (Inflammation) : These results suggest that LPS regulates the opposite NOS expression in H9c2 cells by modifying the redox state of these cells responsible for the NF-kB nuclear translocation via NIK-IKKa/ß-IkBa, simultaneous to the inactivation of the PI3K/Akt pathway ... These results suggest that LPS regulates the opposite NOS expression in H9c2 cells by modifying the redox state of these cells responsible for the NF-kB nuclear translocation via NIK-IKKa/ß-IkBa, simultaneous to the inactivation of the PI3K/Akt pathway
Tyagi et al., PloS one 2012 (Osteoporosis) : E2 treatment to mouse calvarial osteoblasts inhibited the IL-17 induced production of osteoclastogenic cytokines and NF-kB translocation
Crone et al., Molecular cancer 2012 (Disease Models, Animal...) : Lysophosphatidic acid (LPA) induces NF-kappaB activation via this pathway and over-expression of miR-146a inhibited LPA induced NF-kappaB activation, reduced LPA induced expression of tumor promoting cytokines and growth factors and inhibited monocyte attraction ... Lysophosphatidic acid (LPA) induces NF-kappaB activation via this pathway and over-expression of miR-146a inhibited LPA induced NF-kappaB activation, reduced LPA induced expression of tumor promoting cytokines and growth factors and inhibited monocyte attraction
Tian et al., Zhongguo Zhong Yao Za Zhi 2012 : AESC obviously inhibits the pathway activation of CPE induced by HSV-2 infection and NF-kappaB required for virus replication
Su et al., Int Immunopharmacol 2012 (Diabetes Mellitus, Experimental...) : The effect of FK506 treatment ( 10µg per eye for one week ) was evaluated by TNF-a, VEGF, iNOS and COX-2 protein levels measurement, neovascularization, and the activation of NF-kB in the retina ... The effect of FK506 treatment ( 10µg per eye for one week ) was evaluated by TNF-a, VEGF, iNOS and COX-2 protein levels measurement, neovascularization, and the activation of NF-kB in the retina
Catanzaro et al., J Biol Regul Homeost Agents 2012 : Western immunoblotting was used to analyze the MMP-13 production in the culture medium and the activation of NF-kB
Takeiri et al., Oncol Res 2012 (Leukemia, T-Cell) : It was screened for a compound that inhibits LPS induced NF-kappaB and NO production in mouse macrophages
Tang et al., Oncol Res 2012 (Colonic Neoplasms) : LPS increased phosphorylation of ERK1/2 and p38 and activated NF-kappaB
Gan et al., Cell Tissue Res 2013 (Inflammation) : Inhibitors of p38MAPK, JAK-STAT, NF-kB , and AP-1 significantly reduced CX3CL1 and CX3CR1 expression ... Inhibitors of p38MAPK, JAK-STAT, NF-kB , and AP-1 significantly reduced CX3CL1 and CX3CR1 expression
Zhao et al., Behav Brain Res 2013 (Diabetes Mellitus, Experimental) : Inhibition of NF-kappaB activation by Pyrrolidine dithiocarbamate partially attenuates hippocampal MMP-9 activation and improves cognitive deficits in streptozotocin induced diabetic rats
Campo et al., Biofactors 2012 (Inflammation) : IL-1beta stimulation significantly up-regulated HA levels, its fragmentation, cAMP, PKA, cytokine levels, and activated NF-kB
Hu et al., J Radiat Res 2013 (Acute Radiation Syndrome) : First, a cell based screening model for an agonist of TLR5 mediated NF-kB pathway was established and then validated by activation of TLR5 mediated NF-kB luciferase reporter activity with a known TLR5 agonist, flagellin
Yu et al., Food Chem 2013 (Inflammation) : Also, the methanol extract and the hexane fraction inhibited LPS induced MAPKs and NF-kB activation associated with the inhibition of iNOS or COX-2 expression
Lim et al., Biol Reprod 2013 (Inflammation...) : To determine whether SIRT6 affects NFKB transcriptional activity, primary amnion cells were transfected with NFKB tagged with luciferase and stimulated with IL1B ... As expected, IL1B induced NFKB transcriptional activity ... As expected, IL1B induced NFKB transcriptional activity
Posadas et al., PloS one 2012 (Neuroblastoma) : AAP also induced NFkB p65 activation by phosphorylation and its translocation to the nucleus, where NFkB p65 increased IL-1ß production ... AAP also induced NFkB p65 activation by phosphorylation and its translocation to the nucleus, where NFkB p65 increased IL-1ß production
Wang et al., J Chemother 2012 (Breast Neoplasms) : Our results showed that TNF-alpha treatment increased Bcl-2 mRNA and protein levels in breast cancer cells, suggesting that Bcl-2 is directly regulated by nuclear factor-kappaB (NF-kappaB) in response to TNF-alpha ... Our results showed that TNF-alpha treatment increased Bcl-2 mRNA and protein levels in breast cancer cells, suggesting that Bcl-2 is directly regulated by nuclear factor-kappaB (NF-kappaB) in response to TNF-alpha ... Silencing of sClu in MDA-MB-231/sClu siRNA cells abrogated TNF-alpha mediated NF-kappaB activation and Bcl-2 overexpression, and rendered the MDA-MB-231/sClu siRNA cells significantly more sensitive to TNF-alpha mediated apoptosis than the parental cells ... Furthermore, overexpression of sClu in MCF-7/sClu cells promoted TNF-alpha mediated NF-kappaB activity and Bcl-2 overexpression, and rendered the MCF-7/Clu cells significantly more resistant to TNF-alpha mediated apoptosis
Pan et al., PloS one 2012 (Disease Models, Animal...) : High serum levels of lipopolysaccharide (LPS) with LPS-MD-2/TLR4 complex activated NF-kb and cytokine cause hepatic necrosis in animal models
Salerno et al., Neuroscience 2013 (Peripheral Nerve Injuries) : TRAF family member associated NF-kappa B activator ( TANK ) expression increases in injured sensory neurons and is transcriptionally regulated by Sox11
Chen et al., Exp Lung Res 2013 (Carcinoma, Non-Small-Cell Lung...) : In addition, we found that the knockdown of NUAK1 suppressed the expression of MMP-2 and MMP-9 and the activation of NF-kB , which can regulate the transcription of MMP-2 and MMP-9 ... In addition, we found that the knockdown of NUAK1 suppressed the expression of MMP-2 and MMP-9 and the activation of NF-kB , which can regulate the transcription of MMP-2 and MMP-9
Feng et al., Int Immunopharmacol 2013 (Diabetes Mellitus...) : Immunofluorescence and Western blotting assays showed that CM1 could attenuate HMGB1 induced intracellular ERK1/2 and NF-kB activation in HUVECs
Zhang et al., Biochem Biophys Res Commun 2013 (Atherosclerosis) : The inhibitory effects of Ang- ( 1-7 ) on Ang II-induced VCAM-1 promoter activity and NF-kappaB nuclear translocation were all reversed by the competitive antagonist of Ang- ( 1-7 ) at the Mas receptor ... Our results suggest that Ang- ( 1-7 ) mediates its affects on ECs through the Mas receptor, and negatively regulates Ang II-induced VCAM-1 expression by attenuating nuclear translocation of NF-kappaB ... Our results suggest that Ang- ( 1-7 ) mediates its affects on ECs through the Mas receptor, and negatively regulates Ang II-induced VCAM-1 expression by attenuating nuclear translocation of NF-kappaB
Kim et al., BMC cancer 2012 (Colorectal Neoplasms...) : Artificial suppression of l-CaD increased susceptibility of colon cancer cells to 5-FU, and caused an increase of p21 and c-PARP, and a decrease of NF-kB and p-mTOR expression
Frey et al., Ann Rheum Dis 2013 (Arthritis, Psoriatic...) : Functionally, IL-36a induced the expression of IL-6 and IL-8 in FLS through p38/NFkB activation ... Functionally, IL-36a induced the expression of IL-6 and IL-8 in FLS through p38/NFkB activation
Liu et al., Wei Sheng Wu Xue Bao 2012 : L. plantarum NDC 75017 could rapidly activate the phosphorylation of NF-kappaB , and the expressions of il-6 and tlr2 were decreased notably after pretreated with pyrrolidine dithiocarbamate
Li et al., Pulm Pharmacol Ther 2013 : EM, EM703 inhibit NF-kB activation induced by oxidative stress from diesel exhaust particle in human bronchial epithelial cells : Importance in IL-8 transcription
Castro et al., J Mol Endocrinol 2013 (Euthyroid Sick Syndromes...) : LPS induced NF-kB activation was associated with a decrease in THRB gene expression only in frontal lobe, adrenal gland, and kidney cortex ... The role of NF-kB in regulating THRB expression during endotoxemia, if any, is restricted to a limited number of tissues
Wu et al., Int J Immunopathol Pharmacol 2012 (Liver Failure, Acute) : In addition, pretreatment mice with PD dose-dependently suppressed tumor necrosis factor-alpha (TNF-alpha) production, myeloperoxidase (MPO) activity, intercellular adhesion molecule-1 ( ICAM-1 ) and endothelial cell adhesion molecule-1 ( ECAM-1 ) expression, caspase-3 activation, and transcription factor nuclear factor-kappa B(NF-kB) activity induced by LPS
de Bittencourt Pasquali et al., Cell Signal 2013 (Lung Neoplasms) : Besides, we observed that NF-kB acted as a downstream effector of p38 in RAGE downregulation by retinol, as NF-kB inhibition by SN50 ( 100µg/mL ) and siRNA to p65 blocked the effect of retinol on RAGE , and p38 inhibitors reversed NF-kB activation
He et al., Bing Du Xue Bao 2012 (Dengue...) : In addition, VEGF induction by DENV2 was significantly impaired by knockdown of TLR3 and interferon-beta promoter stimulator 1 ( IPS-1 ), or by inhibition of ERK, JNK or NF-kappaB
Lee et al., Histochem Cell Biol 2013 : Our results indicate that VEGF, vWF, CD31, pSmad2, NF-kB , WT-1 and Thy-1 were markedly up-regulated by the loss of Runx3
Chung et al., Neurochem Res 2013 : The effect of PPY-Br on the activation of nuclear factor (NF)-kappaB was examined to identify the mechanism involved
Zhang et al., Virology 2013 : Additionally, PKR silencing suppressed NF-kB activation and NF-kB mediated STAT1 phosphorylation in Huh7.5.1 cells and HCV1a persistently infected Huh7.5.1 cells ( 2HDD4 )
Klettner et al., Molecular vision 2013 (MAP Kinase Signaling System) : Inhibition of the transcription factor NFkB diminished VEGF secretion after 6 h ( earliest measured time point ) and remained diminished at all measured time points ( 24 h, 48 h )
Tafani et al., Frontiers in pharmacology 2013 : Interestingly, hypoxia and inflammation have been sequentially bridged in tumors by the discovery that alarmin receptors genes such as RAGE, P2X7, and some TLRs, are activated by HIF1a ; and that, in turn, alarmin receptors strongly activate NFkB and proinflammatory gene expression, evidencing all the hallmarks of the malignant phenotype
Hwang et al., Nitric Oxide 2013 : Glucosamine inhibits lipopolysaccharide stimulated inducible nitric oxide synthase induction by inhibiting expression of NF-kappaB/Rel proteins at the mRNA and protein levels
Ibraim et al., Parasites & vectors 2013 : Nitric oxide and cytokine production, MAPKs ( ERK , p38 and JNK ) and NF-kB activation were evaluated ... Nitric oxide and cytokine production, MAPKs ( ERK, p38 and JNK ) and NF-kB activation were evaluated
Madonna et al., Int J Immunopathol Pharmacol 2013 (Diabetes Mellitus, Type 2...) : These results provide evidence for CD1d mediated NFkB activation and diastolic dysfunction in the hearts of db/db mice ... Therefore, CD1d associated abnormalities of innate immune responses and TNF-alpha production in splenic tissue may contribute to NFkB activation and cardiac dysfunction in type 2 diabetes
Kim et al., Neurol Res 2013 (Inflammation) : Since purinergic signals activate NF-kappaB through the P2X7 receptor (P2X7R), we investigated the roles of P2X7R in neuronal NF-kappaB phosphorylation in the mouse hippocampus under basal conditions and P2X7R deletion following LPS treatment in vivo
Liu et al., Bing Du Xue Bao 2013 : Further investigations indicated that RelB not only promoted the Vpr mediated activation of NF-kappaB reporter gene, but also enhanced the transactivation of HIV LTR
Spadaro et al., PloS one 2013 : Notably, phosphatidylcholine-specific phospholipase C ( PC-PLC ), previously reported to be required for NF-kB mediated CCL2 production induced by gp120 in macrophages, drives both ERK1/2 activation and PI-PLC ß1 nuclear localization induced by gp120 ... Finally, PI-PLC inhibition neither blocks gp120 mediated NF-kB activation nor overall accumulation of CCL2 mRNA, whereas it decreases CCL2 transcript level in the cytoplasm ... Finally, PI-PLC inhibition neither blocks gp120 mediated NF-kB activation nor overall accumulation of CCL2 mRNA, whereas it decreases CCL2 transcript level in the cytoplasm
Cristi-Montero et al., Rev Med Chil 2012 (Disease Models, Animal...) : Aim : To determine if, after one session of acute exercise, expression of TLR-4 and iNOS, and activation of NF-kB are induced in rat cardiac tissue ... Aim : To determine if, after one session of acute exercise, expression of TLR-4 and iNOS , and activation of NF-kB are induced in rat cardiac tissue
Vakkila et al., Drug Discov Today 2005 : IFNgamma amplified IL-1 induced NF-kappaB responses ... NF-kappaB responses to IL-1beta, CD40L , and LPS were donor dependent ( n = 7 ), correlated with the quality of iDC preparations ( p = 0.002 ), and IL-12 p70 production ( p = 0.010 ) ... NF-kappaB responses to IL-1beta, CD40L , and LPS were donor dependent ( n = 7 ), correlated with the quality of iDC preparations ( p = 0.002 ), and IL-12 p70 production ( p = 0.010 ) ... NF-kappaB responses to IL-1beta , CD40L, and LPS were donor dependent ( n = 7 ), correlated with the quality of iDC preparations ( p = 0.002 ), and IL-12 p70 production ( p = 0.010 ) ... NF-kappaB responses to IL-1beta, CD40L, and LPS were donor dependent ( n = 7 ), correlated with the quality of iDC preparations ( p = 0.002 ), and IL-12 p70 production ( p = 0.010 )
Panguluri et al., PloS one 2013 (Diabetes Mellitus...) : This miR-301 mediated regulation of Kv4.2 is independent of NFkB and Irx5 and modulates Kv4.2 by direct binding on Kv4.2 3'untranslated region ( 3'-UTR )
Carta et al., Italian journal of pediatrics 2013 (MAP Kinase Signaling System...) : BEAS-2B cell line ( human bronchial epithelial cells infected with a replication-defective Adenovirus 12-SV40 virus hybrid ) were cultured in the presence of pidotimod, with or without tumor necrosis factor (TNF)-a or zymosan to assess : a ) intercellular adhesion molecule (ICAM)-1 expression, by flow cytometry ; b ) toll-like receptor (TLR)-2 expression and production, by immunofluorescence flow cytometry and western blotting ; d ) interleukin (IL)-8 release, by enzyme linked immunosorbent assay ( ELISA ) ; e ) activated extracellular-signal regulated kinase ( ERK1/2 ) phosphorylation and nuclear factor-kappa B (NF-kB) activation, by western blotting
Cantu et al., Am J Transplant 2013 (Primary Graft Dysfunction) : Three hundred sixty-two gene sets were upregulated, with eight meeting significance ( familywise-error rate, FWER p-value < 0.05 ), including the NOD-like receptor inflammasome ( NLR ; p < 0.001 ), toll-like receptors ( TLR ; p < 0.001 ), IL-1 receptor ( p = 0.001 ), myeloid differentiation primary response gene 88 ( p = 0.001 ), NFkB activation by nontypeable Haemophilus influenzae ( p = 0.001 ), TLR4 ( p = 0.008 ) and TLR 9 ( p = 0.018 )
Tsai et al., PloS one 2013 : This increased secretion of TNF-alpha and IL-6 and activation of NF-kB , ERK, and JNK was significantly inhibited by the addition of either mannan or mannose ... This increased secretion of TNF-alpha and IL-6 and activation of NF-kB , ERK, and JNK was significantly inhibited by the addition of either mannan or mannose
Lappas et al., Biol Reprod 2013 : The effects of these NOD1 and NOD2 ligands were mediated via NFKB, as 1 ) both iE-DAP and MDP significantly increased NFKB activation and 2 ) the NFKB inhibitor BAY 11-7082 attenuated iE-DAP- and MDP induced expression and secretion of prolabor mediators ... Agonist activation of NOD1 and NOD2 by bacterial products leads to NFKB activation and transcription of NFKB induced prolabor genes ... Agonist activation of NOD1 and NOD2 by bacterial products leads to NFKB activation and transcription of NFKB induced prolabor genes
Williams et al., Cell Mol Neurobiol 2013 (Parkinson Disease) : Involvement of NF Kappa B in Potentiated Effect of Mn-containing Dithiocarbamates on MPP ( + ) Induced Cell Death
Jin et al., PloS one 2013 : Moreover, sCD40L strongly stimulates neutrophil oxidative burst via CD40 dependent activation of PI3K/NF-KB , but independent of Mac-1 and PKC?
Yang et al., Eur Rev Med Pharmacol Sci 2013 (Kidney Neoplasms...) : RNA interference suppression of A100A4 reduces the growth and metastatic phenotype of human renal cancer cells via NF-kB dependent MMP-2 and bcl-2 pathway
Lai et al., PloS one 2013 : Decoy ODN analyses revealed that STAT3 and nuclear factor-kB (NF-kB) also played critical roles in the transcriptional regulation of angiotensinogen by IL-6
Oh et al., Molecules (Basel, Switzerland) 2013 (Inflammation...) : BOWE also inhibited the LPS induced degradation of IkB-a, and LPS induced phosphorylation of p65 NF-kB protein
Sangiovanni et al., PloS one 2013 : In vitro, ETs inhibited TNF-a induced NF-kB driven transcription ( IC50 : 0.67-1.73 µg/mL ) and reduced TNF-a induced NF-kB nuclear translocation ( 57 % -67 % at 2 µg/mL ) ... In vitro, ETs inhibited TNF-a induced NF-kB driven transcription ( IC50 : 0.67-1.73 µg/mL ) and reduced TNF-a induced NF-kB nuclear translocation ( 57 % -67 % at 2 µg/mL )
Guo et al., Zhongguo Zhong Xi Yi Jie He Za Zhi 2013 : Compared with the vehicle control group, the NF-kappaB p65 activity, the expressions of STAT3 and VEGF mRNA increased significantly in RSC-364 induced by IL-17 +TNF-alpha ( P < 0.01, P < 0.05 ). Compared with the model group, the NF-kappaB p65 activity, the expressions of STAT3 and VEGF mRNA decreased significantly in the DT containing serum group and the positive control group ( P < 0.01, P < 0.05 ). There was no statistical difference between the two groups ( P > 0.05 )
Ruben et al., New Biol 1989 : Activation of NF-kappa B by the HTLV-I trans-activator protein Tax requires an additional factor present in lymphoid cells ... We show that activation of NF-kappa B by Tax is an indirect process that requires prior activation of a preexistent factor that is present in lymphoid cells
Shirakawa et al., Mol Cell Biol 1989 : In contrast, PKA and PKC did not activate NF-kappa B in nuclear extracts from unstimulated cells
Israël et al., EMBO J 1989 : This strongly suggests that induction of an NF kappa B-like activity is responsible for TNF-alpha stimulation of mouse MHC class I genes
Freimuth et al., J Immunol 1989 : In this report, we show that TNF-alpha activates NF-kappa B and the human immunodeficiency virus enhancer in the Jurkat T leukemia but does not stimulate the IL-2R alpha enhancer
Briskin et al., Science 1988 : TGF-beta inhibited LPS induced kappa transcription but not the activation and in vitro binding of NF-kappa B ... TGF-beta had no effect on IFN-gamma induced kappa transcription, and NF-kappa B was not activated by IFN-gamma
Amoah-Apraku et al., Kidney Int 1995 : Since LPS is only a weak inducer of iNOS in most nonimmune cells, we investigated the role of NF-kappa B in the regulation of iNOS expression in mouse renal epithelial cells ... We conclude that NF-kappa B is necessary but not sufficient for the induction of renal epithelial iNOS expression, and that in contrast to macrophages, c-rel does not appear to play a major role in the regulation of renal epithelial iNOS
Das et al., Mol Cell Biochem 1995 (Adenocarcinoma...) : Thiol modulation of TNF alpha and IL-1 induced MnSOD gene expression and activation of NF-kappa B ... Thiol modulation of TNF alpha and IL-1 induced MnSOD gene expression and activation of NF-kappa B ... TNF alpha and IL-1 each can activate NF-kappa B and induce gene expression of manganese superoxide dismutase ( MnSOD ), a mitochondrial matrix enzyme which can provide critical protection against hyperoxic lung injury ... TNF alpha and IL-1 each can activate NF-kappa B and induce gene expression of manganese superoxide dismutase ( MnSOD ), a mitochondrial matrix enzyme which can provide critical protection against hyperoxic lung injury ... TNF alpha and IL-1 each can activate NF-kappa B and induce gene expression of manganese superoxide dismutase ( MnSOD ), a mitochondrial matrix enzyme which can provide critical protection against hyperoxic lung injury
Scherer et al., Proc Natl Acad Sci U S A 1995 : The inhibitor protein I kappa B alpha controls the nuclear import of the transcription factor NF-kappa B
Jung et al., Ann N Y Acad Sci 1995 : Integration of the signals generated by TCR and CD28 engagement occurs along this pathway, which then bifurcates to induce I kappa B phosphorylation and NF-kappa B activation on the one hand, and JNK activation and c-Jun phosphorylation on the other
Satake et al., Biochem Biophys Res Commun 1995 : Cu2+ was found to inhibit the activation of NF kappa B induced by TNF-alpha , TPA, or H2O2
Das et al., Am J Physiol 1995 : NAC , DTT, and 2-ME each activated the transcription factor NF-kappa B and increased steady-state levels of MnSOD mRNA and enzyme activity in these cells ... SOD and catalase ( 500 U/ml ) plus ethanol ( 1 mM ) did not inhibit activation of NF-kappa B or elevation of steady-state MnSOD mRNA levels by NAC, DTT, or 2-ME
Spink et al., J Biol Chem 1995 : The mechanism by which NF-kappa B activates the iNOS promoter in VSM cells in response to cytokines appears to be markedly different to that operative in macrophages in response to lipopolysaccharide
Kuno et al., J Biol Chem 1995 : Moreover, we show that the peptide, corresponding to the C-terminal acidic domain of I kappa B alpha, blocked the LPS induced NF-kappa B activation as well as inducible phosphorylation of endogenous I kappa B alpha in a cell-free system using THP-1 cells
Sancéau et al., J Biol Chem 1995 : Triggering of the human interleukin-6 gene by interferon-gamma and tumor necrosis factor-alpha in monocytic cells involves cooperation between interferon regulatory factor-1, NF kappa B , and Sp1 transcription factors ... LPS signaling was found to involve NF kappa B activation by the p50/p65 heterodimers ... Synergistic induction of the IL-6 gene by IFN-gamma and TNF-alpha, in monocytic cells, involved cooperation between the IRF-1 and NF kappa B p65 homodimers with concomitant removal of the negative effect of the retinoblastoma control element present in the IL-6 promoter
Mahon et al., J Biol Chem 1995 (Lymphoma, T-Cell...) : The tyrosine kinase inhibitor herbimycin A was found to block NF-kappa B stimulation in response to interleukin-1 and phorbol 12-myristate 13-acetate in EL4.NOB-1 thymoma cells and phorbol 12-myristate 13-acetate in Jurkat T lymphoma cells
Neish et al., J Biol Chem 1995 : The cytokine induced enhancer of the VCAM1 gene requires constitutively bound Sp1 and induced heterodimeric NF-kappa B for maximal promoter activity
Kone et al., Am J Physiol 1995 : Role of NF-kappa B in the regulation of inducible nitric oxide synthase in an MTAL cell line ... LPS + interferon-gamma (IF-gamma) promoted a time dependent increase in nitrite ( a NO metabolite ) and iNOS mRNA and the appearance of NF-kappa B p50 and p65 in nuclear protein extracts ... LPS + interferon-gamma (IF-gamma) promoted a time dependent increase in nitrite ( a NO metabolite ) and iNOS mRNA and the appearance of NF-kappa B p50 and p65 in nuclear protein extracts
Himes et al., Oncogene 1993 : HTLV-1 tax activation of the GM-CSF and G-CSF promoters requires the interaction of NF-kB with other transcription factor families ... HTLV-1 tax activation of the GM-CSF and G-CSF promoters requires the interaction of NF-kB with other transcription factor families
Xie et al., J Biol Chem 1994 : Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase
Tzen et al., Exp Cell Res 1994 : The current studies now show : ( 1 ) the expression of I kappa B alpha can be induced by protein synthesis inhibitors including cycloheximide, anisomycin, and puromycin ; ( 2 ) cycloheximide dependent induction can be blocked by a transcriptional inhibitor ; ( 3 ) double stranded RNA and tumor necrosis factor alpha, which are both known to induce NF kappa B , induce the expression of I kappa B alpha , whereas L-cysteine, which is known to inhibit NF kappa B expression, inhibits I kappa B alpha expression ; and ( 4 ) the induction of I kappa B alpha gene expression is transient, as is the induction of other NF kappa B-inducible genes
Lewis et al., Mol Cell Biol 1994 : Cytokine induced expression of the E-selectin gene requires the promoter binding and interaction of the transcription factors NF-kappa B and ATF ... Mutational analysis of the -127/-118 NF-kappa B site indicates that both NF-kappa B and HMG-I(Y) binding at this site are essential for interleukin-1 induction of the promoter
Delude et al., J Biol Chem 1994 : LPS stimulated translocation of NF-kappa B in CHO/CD14 cells resembled the same response in the murine macrophage-like cell line RAW 264.7
Schulze-Osthoff et al., EMBO J 1994 : Whereas transcription factor NF-kappa B was readily activated by TNF , activation was not induced by triggering APO-1/Fas ... Whereas transcription factor NF-kappa B was readily activated by TNF, activation was not induced by triggering APO-1/Fas ... Whereas transcription factor NF-kappa B was readily activated by TNF, activation was not induced by triggering APO-1/Fas
Xie et al., J Leukoc Biol 1994 : Nuclear factor NF-kappa B/c-rel , interacting with cycloheximide-sensitive protein ( s ) and binding to the NF-kappa Bd site in the iNOS promoter, controls the induction of iNOS by LPS
Viriyakosol et al., J Biol Chem 1995 : The mutants were characterized in three assays : reactivity with anti-CD14 monoclonal antibody, serum dependent LPS binding, and LPS induced activation of NF-kappa B
Chaturvedi et al., Lymphokine Cytokine Res 1994 : Only TNF , LT, and IL-1 activated NF-kappa B ... Only TNF, LT, and IL-1 activated NF-kappa B ... Since interferons have been shown to induce TNF receptors and potentiate TNF mediated cellular responses, we also measured the effect of interferons on TNF induced activation of NF-kappa B ... Under our conditions, all three IFNs potentiated the cytotoxic effects of TNF but had no effect on the TNF dependent NF-kappa B activation
Lofquist et al., Mol Cell Biol 1995 : The demonstration that NF-kappa B can regulate I kappa B alpha/MAD-3 gene transcription in other cell types suggested that the rapid increase in steady-state I kappa B alpha/MAD-3 mRNA levels we observed within 30 min of monocyte adherence would result from NF-kappa B-dependent transcriptional stimulation of the I kappa B alpha/MAD-3 gene ... The demonstration that NF-kappa B can regulate I kappa B alpha/MAD-3 gene transcription in other cell types suggested that the rapid increase in steady-state I kappa B alpha/MAD-3 mRNA levels we observed within 30 min of monocyte adherence would result from NF-kappa B-dependent transcriptional stimulation of the I kappa B alpha/MAD-3 gene
Weber et al., Circulation 1995 : Our data suggest that aspirin inhibits NF-kappa B mobilization, induction of VCAM-1 and E-selectin , and subsequent monocyte adhesion in endothelial cells stimulated by TNF, thereby providing an additional mechanism for therapeutic effects of aspirin ... Our data suggest that aspirin inhibits NF-kappa B mobilization, induction of VCAM-1 and E-selectin, and subsequent monocyte adhesion in endothelial cells stimulated by TNF, thereby providing an additional mechanism for therapeutic effects of aspirin
Hattori et al., Nihon Naibunpi Gakkai Zasshi 1995 : LPS stimulated translocation into the nuclei of NFkB which is believed to play an important role in the induction of iNOS, but IFN did not enhance NFkB activation by LPS
Saura et al., Kidney Int 1995 : Our results establish DX and PDTC as useful tools to study the regulation of NO synthesis in the mesangial cell and glomerulus, and suggest that NF-kappa B is involved in the transcriptional regulation of iNOS in RMC
Goldring et al., Biochem Biophys Res Commun 1995 : We find NF-kappa B/Rel activation ( p50-p50 and p50-p65 ) in RAW 264.7 macrophages after lipopolysaccharide treatment and binding to both NF-kappa B sites in the mouse iNOS promoter
Francis et al., Int Immunol 1995 : Despite the marked similarity in transcription factor induction triggered through CD40 and slg, differences in the intracellular signaling pathways utilized were apparent in that protein kinase C ( PKC ) depletion did not affect CD40 mediated induction of NF-kappa B even as induction by anti-Ig was abolished
Hattori et al., Biochem Mol Biol Int 1995 : Since NFkB plays a critical role in the induction of an isoform of nitric oxide synthase (iNOS) gene by lipopolysaccharide (LPS), we investigated the effect of a vitamin E derivative, pentamethyl-hydroxychromane ( PMC ), which is an extremely potent inhibitor of NFkB activation, on the induction of nitric oxide ( NO ) synthesis and iNOS mRNA by LPS
Wang et al., Biochem Biophys Res Commun 1995 : The inhibitor of NF-kappa B activation, pyrrolidine dithiocarbamate, inhibited the induction of iNOS but not AII
Bedoya et al., Biochem Biophys Res Commun 1995 (Insulinoma...) : Our data suggest that NF-kappa B activation is necessary for iNOS , but not for MnSOD, mRNA expression in insulin producing cells
Jäättelä et al., Oncogene 1995 (Breast Neoplasms) : Interestingly, exposure of MCF-Fas cells to anti-Fas or TNF induced activation of phospholipase A2 (PLA2), while only TNF activated NF-kappa B
Biswas et al., Immunity 1995 : Furthermore, cross linking of CD30 leads to NF-kappa B activation and enhanced HIV transcription
Masamune et al., FEBS Lett 1995 : N, N,N-trimethylsphingosine inhibits interleukin-1 beta induced NF-kappa B activation and consequent E-selectin expression in human umbilical vein endothelial cells ... Electrophoretic mobility shift assay revealed that TMS inhibited IL-1 beta induced NF-kappa B activation, which is essential for E-selectin expression
Weber et al., J Immunol 1995 : Our data suggest that specific phosphorylation following protein tyrosine kinase activation may be required for NF-kappa B mobilization and induction of VCAM-1 and ELAM-1 by TNF ... Our data suggest that specific phosphorylation following protein tyrosine kinase activation may be required for NF-kappa B mobilization and induction of VCAM-1 and ELAM-1 by TNF ... Our data suggest that specific phosphorylation following protein tyrosine kinase activation may be required for NF-kappa B mobilization and induction of VCAM-1 and ELAM-1 by TNF
Parra et al., J Immunol 1995 (Leukemia-Lymphoma, Adult T-Cell) : Our results indicate distinct effects of B7 and LFA-3 costimulation on the activity of AP-1 and NF-kappa B
Gerritsen et al., Am J Pathol 1995 (Inflammation) : Although apigenin did not inhibit TNF-alpha induced nuclear translocation of NF-kappa B ( p50 ( NFKB1 ) /p65 ( RelA ) ) we found this flavonoid did inhibit TNF-alpha induced beta-galactosidase activity in SW480 cells stably transfected with a beta-galactosidase reporter construct driven by four NF-kappa B elements, suggesting an action on NF-kappa B transcriptional activation
Rothe et al., Science 1995 : TRAF2 mediated activation of NF-kappa B by TNF receptor 2 and CD40 ... TRAF2 mediated activation of NF-kappa B by TNF receptor 2 and CD40 ... Overexpression of TRAF2 , but not TRAF1 or TRAF3, was sufficient to induce NF-kappa B activation ... Overexpression of TRAF2, but not TRAF1 or TRAF3, was sufficient to induce NF-kappa B activation ... Overexpression of TRAF2, but not TRAF1 or TRAF3 , was sufficient to induce NF-kappa B activation ... A truncated derivative of TRAF2 lacking an amino-terminal RING finger domain was a dominant negative inhibitor of NF-kappa B activation mediated by TNF-R2 and CD40 ... A truncated derivative of TRAF2 lacking an amino-terminal RING finger domain was a dominant negative inhibitor of NF-kappa B activation mediated by TNF-R2 and CD40
Tozawa et al., Cancer Res 1995 (Neoplasm Metastasis) : Here we demonstrate that IL-1 beta induces nuclear translocation of NF kappa B in human umbilical vein endothelial cells, followed by induction of cell surface expression of E-selectin, intercellular adhesion molecule-1, and vascular adhesion molecule 1, and subsequently augments adhesion of those cancer cells expressing sialyl Lewis X antigen, a ligand to E-selectin
Cockerill et al., Blood 1995 : CsA also suppressed E-selectin, but not vascular cell adhesion molecule-1 ( VCAM-1 ) expression in endothelial cells, even though the E-selectin promoter is activated by NF-kappa B rather than NFAT
Offermann et al., Eur J Biochem 1995 : Incubation of HUVE cells with the synthetic dsRNA, poly ( I ) .poly ( C ), activates PKR with increased autophosphorylation, increased phosphorylation of the translation factor eIF2 alpha , and increased activation of the transcription factor NF-kappa B ... NF-kappa B is also activated by IL-1 beta in HUVE cells, but this activation occurs without increased PKR autophosphorylation or eIF2 alpha phosphorylation
Nishiya et al., FEBS Lett 1995 (Glioma) : Herbimycin A suppresses NF-kappa B activation and tyrosine phosphorylation of JAK2 and the subsequent induction of nitric oxide synthase in C6 glioma cells ... LPS activated NF-kappa B , and this effect was inhibited by pretreatment with herbimycin A ... These results suggest that herbimycin A suppresses iNOS induction by inhibition of both NF-kappa B activation caused by LPS, and tyrosine-phosphorylation of JAK2 caused by IFN-gamma in C6 glioma cells
Westwick et al., J Biol Chem 1995 (Second Messenger Systems) : Tumor necrosis factor alpha (TNF alpha) activates the stress activated protein kinases ( SAPKs, also known as Jun nuclear kinases or JNKs ) resulting in the stimulation of AP-1 dependent gene transcription and induces the translocation of NF kappa B to the nucleus resulting in the stimulation of NF kappa B-dependent gene transcription ... TNF alpha also potently stimulates NF-kappa B DNA binding activity and transcriptional activity, but these effects are not mimicked by addition of C2-ceramide or sphingomyelinase to intact cells
Chen et al., J Immunol 1995 : We demonstrate that the appearance of NF-kappa B DNA binding activity in the nucleus of TNF-alpha stimulated HUVEC is associated with the rapid phosphorylation and subsequent degradation of I kappa B-alpha , the cytoplasmic inhibitor of NF-kappa B
Scheinman et al., Science 1995 : The activation of NF-kappa B involves the targeted degradation of its cytoplasmic inhibitor, I kappa B alpha , and the translocation of NF-kappa B to the nucleus ... Stimulation by tumor necrosis factor causes the release of NF-kappa B from I kappa B alpha
Chen et al., Biochem Biophys Res Commun 1995 : The results indicated that silica and LPS can significantly induce the activation of NF-kappa B as well as elicit enhanced production of NO in RAW 264.7 cells as part of an early inflammatory response mechanism
Chen et al., Biochem Biophys Res Commun 1995 (Inflammation) : Furthermore, activation of NF-kappa B by silica and LPS appears to be mediated by different signal transduction pathways
Warzocha et al., Eur Cytokine Netw 1995 (Second Messenger Systems) : Since induction of AP-1, IRF-1 and IRF-2 as well as NF-GMa proceeds through translational event, the posttranslational TNF/LT-driven activation of NF-kappa B remains the only cellular event identified so far that serves as a direct target in their signaling cascade
Albrecht et al., J Inflamm 1995 : However, inhibition of TNF-alpha mRNA accumulation by cAMP inducers had no effect on NF-kappa B induction ... We conclude that NF-kappa B translocation is necessary, but not sufficient for the transcriptional induction of the TNF-alpha gene by LPS in macrophages or by phorbol ester and ionomycin in T lymphocytes
Ares et al., Arterioscler Thromb Vasc Biol 1995 : In contrast, the radical dependent transcription factor nuclear factor-kappa B (NF-kappa B) was not activated by Ox-LDL, and at a concentration of 50 micrograms/mL, Ox-LDL inhibited lipopolysaccharide induced activation of NF-kappa B
Matthews et al., Int J Biochem Cell Biol 1995 : It was recently shown that increased I kappa B-alpha expression in the cytoplasm leads to I kappa B-alpha accumulating in the nuclear compartment, removing template bound NF-kappa B, and reducing NF-kappa B-dependent transcription
Kwon et al., Endocrinology 1995 (Insulinoma...) : Inhibitors of NFkappaB , diethyldithiocarbamate, pyrrolidine dithiocarbamate, and N-acetyl cysteine prevent IL-1 induced iNOS expression at the level of messenger RNA, protein, and nitrite generation ... IL-1 induces a time dependent translocation of NFkappaB from cytosol to nucleus, with maximal translocation observed approximately 15-30 min after IL-1 treatment, as determined by electrophoretic mobility shift assays ... IL-1 induced translocation of NF kappa B was blocked by 100 microns 100 microM diethyldithiocarbamate or 100 microM pyrrolidine dithiocarbamate, further establishing a critical role for NF kappa B in the induction of iNOS by IL-1 in rat pancreatic beta-cells ... IL-1 induced translocation of NF kappa B was blocked by 100 microns 100 microM diethyldithiocarbamate or 100 microM pyrrolidine dithiocarbamate, further establishing a critical role for NF kappa B in the induction of iNOS by IL-1 in rat pancreatic beta-cells ... Activation of tyrosine kinase appears to precede NF kappa B activation, as the tyrosine kinase inhibitor genistein ( 100 microM ) blocks IL-1 induced translocation of NF kappa B
McDonald et al., Eur J Immunol 1995 (Hodgkin Disease...) : Moreover, in human T helper ( Th ) clones functionally characterized as being of the type 0, type 1 and type 2 ( 28 %, < 1 % und 93 % CD30+, respectively ), the extent of CD30 mediated NF-kappa B activation correlated with the proportion of CD30+ cells
Clarke et al., Eur J Immunol 1995 : Interleukin-4 inhibits kappa light chain expression and NF kappa B activation but not I kappa B alpha degradation in 70Z/3 murine pre-B cells ... We investigated whether IL-4 could inhibit the up-regulation of kappa light chain and activation of NF kappa B by LPS in 70Z/3 ... We investigated whether IL-4 could inhibit the up-regulation of kappa light chain and activation of NF kappa B by LPS in 70Z/3 ... Additionally, electrophoretic mobility shift assays confirmed this effect on LPS induced NF kappa B DNA binding activity in the nucleus ... IL-4 was also found partially to inhibit NF kappa B activity induced by tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta (IL-1 beta) ... IL-4 was also found partially to inhibit NF kappa B activity induced by tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta (IL-1 beta) ... IL-4 was also found partially to inhibit NF kappa B activity induced by tumor necrosis factor-alpha (TNF-alpha) and interleukin-1-beta (IL-1 beta)
Beg et al., Genes Dev 1995 : Our results indicate that I kappa b beta, but not I kappa B alpha , is required for the signal dependent activation of NF-kappa B in fibroblasts ... Our results indicate that I kappa b beta , but not I kappa B alpha, is required for the signal dependent activation of NF-kappa B in fibroblasts ... However, I kappa B alpha is required for the postinduction repression of NF-kappa B in fibroblasts
Bertrand et al., J Biol Chem 1995 : We examined the effect of insulin on nuclear factor kappa B (NF-kappa B) activity in Chinese ovary ( CHO ) cells overexpressing wild-type ( CHO-R cells ) or -defective insulin receptors mutated at Tyr1162 and Tyr1163 autophosphorylation sites ( CHO-Y2 cells ) ... In CHO-R cells, insulin caused a specific, time-, and concentration dependent activation of NF-kappa B ... Moreover, insulin activation of NF-kappa B-driven luciferase reporter gene expression was blocked in CHO-R cells expressing a Raf-1 dominant negative mutant ... Insulin activation of NF-kappa B : 1 ) was related to insulin receptor number and tyrosine kinase activity since it was markedly reduced in parental CHO cells which proved to respond to insulin growth factor-1 and phorbol 12-myristate 13-acetate ( PMA ) activation, and was dramatically decreased in CHO-Y2 cells ; 2 ) persisted in the presence of cycloheximide and was blocked by pyrrolidine dithiocarbamate, aspirin and sodium salicylate, three compounds interfering with I kappa B degradation and/or NF-kappa B.I kappa B complex dissociation ; 3 ) was independent of both PMA-sensitive and atypical ( zeta ) protein kinases C ; and 4 ) was dependent on Raf-1 kinase activity since insulin stimulated NF-kappa B DNA binding activity was inhibited by 8-bromo-cAMP, a Raf-1 kinase inhibitor
Laegreid et al., J Biol Chem 1995 (Adenocarcinoma...) : Pretreatment with TNF for 72 h inhibited both TNF, tumor necrosis factor receptor (TNFR) p55, TNFR p75 , and LPS mediated activation of nuclear factor -kappa B (NF kappa B) , whereas pretreatment with LPS only inhibited the LPS response ... Pretreatment with TNF for 72 h inhibited both TNF, tumor necrosis factor receptor (TNFR) p55, TNFR p75, and LPS mediated activation of nuclear factor -kappa B (NF kappa B) , whereas pretreatment with LPS only inhibited the LPS response ... Pretreatment with TNF for 72 h inhibited both TNF, tumor necrosis factor receptor (TNFR) p55 , TNFR p75, and LPS mediated activation of nuclear factor -kappa B (NF kappa B) , whereas pretreatment with LPS only inhibited the LPS response
Colasanti et al., J Biol Chem 1995 (Nervous System Diseases) : Here, we report that pretreatment of human ramified microglial cells with nearly physiological levels of exogenous NO prevents lipopolysaccharide (LPS)/tumor necrosis factor alpha (TNF alpha)-inducible NO synthesis, because by affecting NF-kappa B activation it inhibits inducible Ca ( 2+ ) -independent NO synthase isoform (iNOS) mRNA expression
Bellas et al., J Clin Invest 1995 : Microinjection of either purified I kappa B-alpha , a naturally occurring specific inhibitor of NF-kappa B/Rel activity, or double stranded oligonucleotides harboring wild type, but not non binding mutants of NF-kappa B elements selectively inhibited SMC proliferation
Thieblemont et al., J Immunol 1995 (HIV Infections) : The effect of LPS and TNF-alpha is mediated by their ability to induce nuclear translocation of the DNA binding heterodimer NF-kappa B ( p50/p65 ), which binds to a specific sequence in the HIV-long terminal repeat ... The effect of LPS and TNF-alpha is mediated by their ability to induce nuclear translocation of the DNA binding heterodimer NF-kappa B ( p50/p65 ), which binds to a specific sequence in the HIV-long terminal repeat ... Translocation of NF-kappa B p50/p65 was also observed following stimulation of CR1 or CR3 of uninfected peripheral blood monocytes from HIV-seronegative donors ... TNF-alpha did not mediate the translocation of NF-kappa B p50/p65 induced by triggering of complement receptors
Yao et al., Environ Health Perspect 1995 : Linker scanning mutational analysis of transcription factor binding sites in the promoter revealed that both the NF kappa B and an adjacent aromatic hydrocarbon response element ( AhRE ) are required for TCDD dependent CAT expression
Adam et al., J Biol Chem 1995 : In addition, PDGF induced activation of the PDGFR-TR55 chimeric receptor resulted in nuclear translocation of NF-kappa B
Croston et al., J Biol Chem 1995 : NF-kappa B activation by interleukin-1 (IL-1) requires an IL-1 receptor associated protein kinase activity ... By receptor mutagenesis studies, we have identified a region of the cytoplasmic domain of IL-1RI that is required for both IL-1 mediated NF-kappa B activation and IL-1 dependent activation of a receptor associated protein kinase activity we term IRAK ... Therefore, we propose that IRAK activation is a necessary step in the activation of NF-kappa B by IL-1
Peng et al., J Biol Chem 1995 : Electrophoretic mobility shift assays demonstrated that activation of NF-kappa B by L-NMA, ox-LDL, and TNF alpha was attenuated by GSNO and SNP, but not by glutathione or cGMP analogues ... Electrophoretic mobility shift assays demonstrated that activation of NF-kappa B by L-NMA, ox-LDL , and TNF alpha was attenuated by GSNO and SNP, but not by glutathione or cGMP analogues
Sun et al., Mol Cell Biol 1995 : Although a kappa B motif partially overlaps with this element and those genotoxic agents activate NF-kappa B, it does not play a major role in p53 genotoxic stress response : NF-kappa B p65 expression did not induce significant p53 promoter activation, and NF-kappa B inhibitors ( N-acetyl cysteine and I kappa B alpha ) did not inhibit genotoxic stress-inducible p53 promoter activation
Sun et al., J Biol Chem 1995 : Activation of NF-kappa B by phosphatase inhibitors involves the phosphorylation of I kappa B alpha at phosphatase 2A-sensitive sites ... Activation of NF-kappa B by various cellular stimuli involves the phosphorylation and subsequent degradation of its inhibitor, I kappa B alpha , although the underlying mechanism remains unclear
Tran-Thi et al., Hepatology 1995 : Using primary cultures of rat Kupffer cells the role of NF-kappa B and activator protein 1 (AP-1) in the expression of the tumor necrosis factor-alpha (TNF-alpha) gene by lipopolysaccharide (LPS) was investigated ... Of all substances found to interfere with TNF-alpha production by Kupffer cells ( pyrrolidine dithiocarbamate, dexamethasone, prostaglandin E2, interleukin [ IL]-4, IL-10, and transforming growth factor-beta [ TGF-beta ] ) only pyrrolidine dithiocarbamate was able to completely inhibit the activation of NF-kappa B by LPS ... Although not abrogating the LPS activation of NF-kappa B , dexamethasone inhibited that of AP-1 ... The results indicate a direct participation of NF-kappa B in the regulation of TNF-alpha synthesis and a differential effect of LPS on NF-kappa B and AP-1, respectively ... The results indicate a direct participation of NF-kappa B in the regulation of TNF-alpha synthesis and a differential effect of LPS on NF-kappa B and AP-1 , respectively ... The results indicate a direct participation of NF-kappa B in the regulation of TNF-alpha synthesis and a differential effect of LPS on NF-kappa B and AP-1, respectively
Jenkins et al., J Immunol 1995 (Leukemia-Lymphoma, Adult T-Cell) : We show in this work that mutations in either the purine-rich region of the CLEO element or the NF-kappa B site result in reduced PMA/Ca2+ activation of a 620-bp human GM-CSF promoter-luciferase reporter construct in Jurkat T cells by 65 % and 50 %, respectively
Dhib-Jalbut et al., J Immunol 1995 : Induction of IFN-beta gene expression by virus requires binding of NF-kappa B to the positive regulatory domain II element of the IFN-beta promoter ... Our studies indicate that MV, TNF-alpha , or PIPC induces NF-kappa B ( p50 and p65 subunits ) binding to positive regulatory domain II in the glioma cell line
Los et al., EMBO J 1995 : IL-2 gene expression and NF-kappa B activation through CD28 requires reactive oxygen production by 5-lipoxygenase ... In primary T lymphocytes we show that CD28 ligation leads to the rapid intracellular formation of reactive oxygen intermediates ( ROIs ) which are required for CD28 mediated activation of the NF-kappa B/CD28-responsive complex and IL-2 expression
Duckett et al., J Biol Chem 1995 : Here we examine the inductions of NF-kappa B in serum deprived and cycling cells in response to tumor necrosis factor-alpha (TNF-alpha) ... These data reveal that NF-kappa B is rapidly induced by TNF-alpha in both proliferating and arrested cells and suggest that distinct activation pathways can lead to cell cycle dependent or -independent induction of NF-kappa B
Menon et al., J Biol Chem 1995 : Inhibitors of phosphotyrosyl protein phosphatases, pervanadate and phenylarsine oxide, abrogate tumor necrosis factor (TNF) induced nuclear factor kappa B (NF-kappa B) nuclear translocation in transformed cell lines ( U-937 and Jurkat ) and primary fibroblasts ( MRC-5 and REF ) ... Treatment of cells with pervanadate inhibited TNF induced I kappa B-alpha phosphorylation and degradation, whereas the serine protease inhibitors tosylphenylalanyl chloromethyl ketone and N alpha-p-tosyl-L-lysine chloromethyl ketone prevented TNF induced I kappa B-alpha degradation and NF-kappa B nuclear translocation, but not the TNF induced phosphorylation of I kappa B-alpha ... The data suggest that TNF and okadaic acid induce the activation of a putative protein-tyrosine phosphatase ( s ), leading to I kappa B-alpha serine phosphorylation and degradation and NF-kappa B nuclear translocation
Roebuck et al., J Biol Chem 1995 : The results indicate that H2O2 activates ICAM-1 transcription through AP-1/Ets elements within the ICAM-1 promoter, which are distinct from NF-kappa B-mediated ICAM-1 expression induced by TNF alpha
Sun et al., J Biol Chem 1995 : TIMP-3 gene expression was inducible by AP-1 and NF-KB activators, 12-O-tetradecanoylphorbol-13-acetate, and tumor necrosis factor-alpha only in preneoplastic cells with an induction peak at 2 h post-treatment, suggesting classification of mTIMP-3 as a member of the immediate early gene family
Douvdevani et al., Kidney Int 1995 : In addition, the CDS inhibited DNA binding activity of NF-kappa B that is probably involved in regulation of LPS mediated transcriptional activation of the TNF alpha gene
Tepper et al., J Immunol 1995 : Analysis of nuclear and cytoplasmic NF-kappa B expression using electrophoretic mobility shift analysis and Western analysis, revealed that DSG blocked LPS induced NF-kappa B nuclear translocation, but had no effect on cytoplasmic NF-kappa B levels ... Analysis of nuclear and cytoplasmic NF-kappa B expression using electrophoretic mobility shift analysis and Western analysis, revealed that DSG blocked LPS induced NF-kappa B nuclear translocation, but had no effect on cytoplasmic NF-kappa B levels
Caldenhoven et al., Mol Endocrinol 1995 : Despite the importance of the DNA binding domain, we found that the transcriptional repression of NF-kappa B , mediated by GR, is not caused by binding of GR to the ICAM-1 NF-kappa B element, but by a physical interaction between the GR and RelA protein
Higuchi et al., Blood 1995 (Leukemia, Myeloid) : TPCK also blocked TNF induced activation of nuclear factor (NF)-kappa B
Gautam et al., Blood 1995 : Furthermore, IL-1 alpha induces activation of transcription factor nuclear factor (NF)-kB , and sodium salicylate suppresses it in a dose dependent manner
Wood et al., Neurosci Lett 1995 : Regulation of NF-kappa B activity in rat dorsal root ganglia and PC12 cells by tumour necrosis factor and nerve growth factor
Maggirwar et al., Oncogene 1995 : Activation of NF-kappa B/Rel by Tax involves degradation of I kappa B alpha and is blocked by a proteasome inhibitor ... Activation of NF-kappa B/Rel by Tax involves degradation of I kappa B alpha and is blocked by a proteasome inhibitor ... In the present study, we demonstrate that Tax mediated activation of NF-kappa B/Rel can be inhibited by a proteasome inhibitor, suggesting the involvement of proteolytic reactions in this Tax-specific activation pathway ... Together, these studies suggest that Tax activation of NF-kappa B/Rel may involve induction of phosphorylation and subsequent proteasome mediated degradation of the inhibitor I kappa B alpha ... Together, these studies suggest that Tax activation of NF-kappa B/Rel may involve induction of phosphorylation and subsequent proteasome mediated degradation of the inhibitor I kappa B alpha
Howard et al., Proc Natl Acad Sci U S A 1993 : Transcriptional activation of the HIV long terminal repeat and subsequent increase in virus production are linked to TNF activation of the cellular transcription factor NF-kappa B
Sherman et al., Biochem Biophys Res Commun 1993 : These findings suggest iNOS activity in macrophages treated with LPS + IFN gamma requires NF-kappa B activation
Joshi-Barve et al., J Biol Chem 1993 (Melanoma) : Using gel retardation and transient expression assays, we show that IL-1 causes protein tyrosine phosphorylation dependent activation of NF-kappa B enhancer binding protein, which then induces transcription of the gro-genes via an NF-kappa B site located 76 base pairs upstream from the cap site
Deisher et al., FEBS Lett 1993 : Interleukin-1 beta, TNF-alpha , and LPS induce nuclear factor (NF)-kappa B-like binding activity, which precedes VCAM-1 transcription ... Interleukin-1 beta , TNF-alpha, and LPS induce nuclear factor (NF)-kappa B-like binding activity, which precedes VCAM-1 transcription ... Interleukin-1 beta, TNF-alpha, and LPS induce nuclear factor (NF)-kappa B-like binding activity, which precedes VCAM-1 transcription
Shu et al., Mol Cell Biol 1993 : In contrast, NF-kappa B2 inhibited functional activation of the VCAM reporter by p65
Kaszubska et al., Mol Cell Biol 1993 : We previously reported that NF-kappa B and a complex we referred to as NF-ELAM1 play a central role in cytokine induced expression of the E-selectin gene
Brasier et al., Kidney Int 1994 (Acute-Phase Reaction) : NF-IL6 , a less potent transactivator, attenuates NF-kappa B mediated transcription late in the evolution of the acute-phase response
Suzuki et al., Oncogene 1995 : These findings suggest that Tax induces dissociation of and/or inhibits the formation of NF-kappa B/I kappa B-alpha complexes, resulting in release of NF-kappa B and destabilization of I kappa B-alpha ... These Tax effects counteract the negative feedback control of NF-kappa B by I kappa B-alpha and contribute to constitutive activation of NF-kappa B in Tax transfected and HTLV-1 infected cells
Bursten et al., Circ Shock 1994 (Inflammation...) : TNF alpha induced sphingomyelin hydrolysis, PLC mediated PC hydrolysis, and DG kinase mediated PA formation or TNF alpha induced NF-kappa B activation and apoptosis are not inhibited by LSF
O'Connell et al., J Biol Chem 1995 (Lymphoma, T-Cell) : As the TNF gene has an NF kappa B binding motif, an autocrine loop involving TNF induction of NF kappa B is therefore likely in these cells
McKean et al., Int Immunol 1995 : Gel shift analyses demonstrate that NF-kappa B nuclear translocation is stimulated primarily by IL-1 rather than by antigen receptor signals ... These results suggest that IL-1 induces both NF-kappa B nuclear translocation and the synthesis of a protein ( s ) responsible for terminating NF-kappa B-DNA interaction in the nucleus ... Antigen receptor signals prolong NF-kappa B-DNA interaction, probably by functionally antagonizing the IL-1 induced synthesis of a protein ( s ) responsible for the transient NF-kappa B-DNA interaction and consequently synergistically enhance IL-1 induced NF-kappa B-dependent gene transcription
Wang et al., J Biol Chem 1995 : Interleukin (IL)-10 inhibits nuclear factor kappa B (NF kappa B) activation in human monocytes ... Using electrophoretic mobility shift assays ( EMSA ), we now show that, in monocytes stimulated with LPS or TNF alpha, IL-10 inhibits nuclear stimulation of nuclear factor kappa B (NF kappa B) , a transcription factor involved in the expression of inflammatory cytokine genes ... IL-4, another cytokine that inhibits cytokine mRNA accumulation in monocytes, shows little inhibitory effect on LPS induced NF kappa B activation
Takeuchi et al., Proc Natl Acad Sci U S A 1995 : These findings establish that, as with other endothelial cell adhesion molecules, transcriptional induction of MAdCAM-1 by TNF-alpha requires activated NF-kappa B proteins ... These findings establish that, as with other endothelial cell adhesion molecules, transcriptional induction of MAdCAM-1 by TNF-alpha requires activated NF-kappa B proteins
Arakawa et al., FEBS Lett 1995 (Leukemia, Erythroblastic, Acute) : The transcriptional regulation of human arachidonate 12-lipoxygenase gene by NF kappa B/Rel
Singh et al., J Biol Chem 1995 : Protein-tyrosine phosphatase inhibitors block tumor necrosis factor dependent activation of the nuclear transcription factor NF-kappa B ... How TNF activates NF-kappa B , however, is not well understood ... We examined the role of protein phosphatases in the TNF dependent activation of NF-kappa B ... Since both dithiothreitol and dimercaptopropanol reversed the inhibitory effect of PAO, critical sulfhydryl groups in the PTPase must be involved in NF-kappa B activation by TNF
Ray et al., J Biol Chem 1995 : In transfection experiments, overexpression of I kappa BR significantly inhibited NF-kappa B-dependent transcription from the Ig kappa enhancer
Arenzana-Seisdedos et al., Mol Cell Biol 1995 : Inducible nuclear expression of newly synthesized I kappa B alpha negatively regulates DNA binding and transcriptional activities of NF-kappa B ... However, NF-kappa B-dependent transcription of the I kappa B alpha gene leads to rapid resynthesis of the I kappa B alpha protein and inhibition of NF-kappa B-dependent transcription
Brockman et al., Mol Cell Biol 1995 : To explore these I kappa B alpha dependent mechanisms for NF-kappa B induction, we identified novel mutants of I kappa B alpha that uncouple its inhibitory and signal transducing functions in human T lymphocytes
Ledbetter et al., Circ Shock 1994 : Activation of NF-kappa B occurred in RAJI cells by G28-5 or by gp39 treatment, and was CD40 crosslinking dependent ... These results suggest that activation of NF-kappa B is involved in some CD40 receptor signals and may be related to CD40 effects on stimulation or inhibition of apotosis
Neumann et al., EMBO J 1995 : These results indicate that PKA mediated suppression of NF-kappa B activity plays an important role in the control of activation of peripheral T lymphocytes
Suzuki et al., Biochem Biophys Res Commun 1995 : Transient overexpression of catalase does not inhibit TNF- or PMA induced NF-kappa B activation ... Overexpression of catalase , however, did not block TNF- or PMA induced NF-kappa B activation
Hsu et al., Cell 1995 : Overexpression of TRADD leads to two major TNF induced responses, apoptosis and activation of NF-kappa B ... Overexpression of TRADD leads to two major TNF induced responses, apoptosis and activation of NF-kappa B ... However, NF-kappa B activation by TRADD is not inhibited by crmA expression, demonstrating that the signaling pathways for TNF induced cell death and NF-kappa B activation are distinct ... However, NF-kappa B activation by TRADD is not inhibited by crmA expression, demonstrating that the signaling pathways for TNF induced cell death and NF-kappa B activation are distinct
Kazmi et al., J Cell Biochem 1995 : TNF-alpha induced NF kappa B was also inhibited by tepoxalin in HeLa cells, while relatively less marked inhibition was observed in Jurkat cells
Hwang et al., Cancer Biochem Biophys 1995 : The intranuclear transcription factor complexes contained two NF-kappa B heterodimers, p50/RelA and p50/c-rel. Taxol induced nuclear translocation of NF-kappa B was inhibited by pyrrolidine dithiocarbamate, an antioxidant, but not by cycloheximide, a protein synthesis inhibitor
Traenckner et al., EMBO J 1995 : Post-translational activation of the higher eukaryotic transcription factor NF-kappa B requires both phosphorylation and proteolytic degradation of the inhibitory subunit I kappa B-alpha ... In this study, we have identified serines 32 and 36 in human I kappa B-alpha as essential for the control of I kappa B-alpha stability and the activation of NF-kappa B in HeLa cells
Thanos et al., Mol Cell Biol 1995 (Virus Diseases) : We conclude that the p50/p65 heterodimer is responsible for the NF-kappa B-dependent activation of the IFN-beta gene promoter in response to virus infection
Kanno et al., Proc Natl Acad Sci U S A 1994 : Human T-cell leukemia virus type I Tax-protein mediated activation of NF-kappa B from p100 ( NF-kappa B2 ) -inhibited cytoplasmic reservoirs ... Induction of these genes is mediated, at least in part, through Tax dependent posttranslational activation of NF-kappa B , typically heterodimers of p50 ( NF-kappa B1 ) and p65 ( RelA ) ... The results demonstrate a mechanism by which Tax may activate NF-kappa B in T cells
Hirano et al., Biochem Biophys Res Commun 1995 : Further, the kinase negative nPKC epsilon partially suppresses endogenous NF-kappa B activity ... These results suggest that nPKC epsilon is specifically involved in the activation of NF-kappa B when cells are treated with TPA
Scheinman et al., Mol Cell Biol 1995 : In transfection experiments, DEX treatment in the presence of cotransfected glucocorticoid receptor ( GR ) inhibits NF-kappa B p65 mediated gene expression and p65 inhibits GR activation of a glucocorticoid response element ... In HeLa cells, DEX activation of endogenous GR is sufficient to block tumor necrosis factor alpha or interleukin 1 activation of NF-kappa B at the levels of both DNA binding and transcriptional activation
Ding et al., J Leukoc Biol 1995 : The effects of bacterial lipopolysaccharide (LPS) on macrophage gene expression are mediated in part by its ability to induce activation of transcription factor NF-kappa B
Brennan et al., Biochim Biophys Acta 1995 : The results from this study therefore suggest that the reactive oxygen model of NF kappa B activation may be restricted to certain cell types and that the presence of such a system is not required for the activation of NF kappa B by IL1 and TNF ... The results from this study therefore suggest that the reactive oxygen model of NF kappa B activation may be restricted to certain cell types and that the presence of such a system is not required for the activation of NF kappa B by IL1 and TNF
Pimentel-Muiños et al., Eur J Immunol 1995 : The regulation of nuclear factor (NF)-kappa B activation by the T cell receptor (TcR)/CD3 complex in primary human T cells has been studied at various times after activation ... These results indicate that the regulation of NF-kappa B activation during T cell activation by TcR/CD3 signals is biphasic : TcR/CD3 triggers its immediate translocation, which is transient if no TNF-alpha is present
Williams et al., Eur J Immunol 1995 : Reporter gene experiments demonstrated that NF-kappa B transcriptional activity is not affected by expression of activated p21ras
Huen et al., Oncogene 1995 : The Epstein-Barr virus latent membrane protein-1 (LMP1) mediates activation of NF-kappa B and cell surface phenotype via two effector regions in its carboxy-terminal cytoplasmic domain
Atwood et al., AIDS Res Hum Retroviruses 1994 : Our results suggest that TNF-alpha stimulation of HIV-1 gene expression in primary cultures of human fetal glial cells is mediated by an increase in binding of NF-kappa B ( p50/p65 ) to the HIV-1 LTR
Lin et al., J Biol Chem 1995 : Transcriptional analysis in vitro demonstrated that 1 ) various dimeric complexes of NF-kappa B differentially stimulated transcription through the human immunodeficiency virus enhancer or PRDII up to 20-fold ; 2 ) recombinant I kappa B alpha specifically inhibited NF-kappa B-dependent transcription in vitro ; and 3 ) different NF-kappa B complexes and interferon regulatory factor 1 cooperated to stimulate transcription in vitro through the PRDIII-PRDI-PRDII virus-inducible regulatory domains of the IFN-beta promoter ... Transcriptional analysis in vitro demonstrated that 1 ) various dimeric complexes of NF-kappa B differentially stimulated transcription through the human immunodeficiency virus enhancer or PRDII up to 20-fold ; 2 ) recombinant I kappa B alpha specifically inhibited NF-kappa B-dependent transcription in vitro ; and 3 ) different NF-kappa B complexes and interferon regulatory factor 1 cooperated to stimulate transcription in vitro through the PRDIII-PRDI-PRDII virus-inducible regulatory domains of the IFN-beta promoter
McElhinny et al., J Virol 1995 (HIV Infections) : Transcriptional activity of p105 is also increased in infected cells and is also mediated by NF-kappa B through a specific kappa B motif
Murakami et al., Virology 1995 : HTLV-1 Tax enhances NF-kappa B2 expression and binds to the products p52 and p100, but does not suppress the inhibitory function of p100 ... Here, we report that Tax enhances expression of NF-kappa B2 at the mRNA level and proteins ; the effect was more apparent on the p52 expression than on its precursor p100, suggesting post-translational regulation
Lawrence et al., Infect Immun 1995 (Shock, Septic) : Diphosphoryl lipid A from Rhodobacter sphaeroides transiently activates NF-kappa B but inhibits lipopolysaccharide induction of kappa light chain and Oct-2 in the B-cell lymphoma line 70Z/3 ... RsDPLA completely repressed the long-term activation of NF-kappa B observed after 24 h of Salmonella typhosa LPS treatment and antagonized activation of oct-2 mRNA expression
Brown et al., Science 1995 : I kappa B-alpha inhibits transcription factor NF-kappa B by retaining it in the cytoplasm
Li et al., EMBO J 1995 : The same results were obtained in vivo using transfection assays ; 14-3-3 enhanced both c-Raf-1- and Raf-1 kinase domain stimulated expression of AP-1- and NF-kappa B-dependent reporter genes and accelerated Raf-1 kinase domain triggered differentiation of PC12 cells
Herrero et al., J Virol 1995 : NF-kappa B , a transcription factor controlling the expression of genes involved in cell activation and growth control, has been shown to be activated by LMP-1
Hurme et al., Immunol Lett 1994 : Effect of interleukin-10 on NF-kB and AP-1 activities in interleukin-2 dependent CD8 T lymphoblasts
Baur et al., J Immunol 1994 (HIV Infections) : Effects of CD45 on NF-kappa B
Kumar et al., Proc Natl Acad Sci U S A 1994 : We present direct evidence that PKR can phosphorylate I kappa B-alpha ( MAD-3 ) and activate NF-kappa B DNA binding activity in vitro
Antoni et al., Virology 1994 : HIV-1 expression was activated from J delta K cells by treatment with phorbol myristate acetate ( PMA ), sodium butyrate ( NaB ), or hexamethylene bisacetamide ( HMBA ), but not tumor necrosis factor alpha (TNF-alpha), confirming the role of NF-kappa B in mediating TNF-alpha induction of HIV transcription
Ivanov et al., J Immunol 1994 : These experiments also demonstrated a key role for NF-kappa B and AP-1 in the transcriptional regulation of the IL-2 gene by TCR- and Ly6A/E mediated signaling
Koong et al., Cancer Res 1994 : Furthermore, although conditions of low oxygen can also activate mitogen activated protein kinases ( ERK1 and ERK2 ), these kinases do not appear to be involved in regulating NF-kappa B by low oxygen conditions, as dominant negative mutants of mitogen activated protein kinase do not inhibit NF-kappa B activation by hypoxia
Naumann et al., EMBO J 1994 : As an in vitro model we show that phosphorylation of p105 impedes its ability to interact with NF-kappa B , as has been shown before for I kappa B alpha
Shibanuma et al., FEBS Lett 1994 : Inhibition by N-acetyl-L-cysteine of interleukin-6 mRNA induction and activation of NF kappa B by tumor necrosis factor alpha in a mouse fibroblastic cell line, Balb/3T3 ... ( ii ) NF kappa B binding activity in this cell line was also increased by TNF alpha , and the increase was inhibited in the presence of NAC
Reddy et al., J Biol Chem 1994 : The post-receptor binding events that culminate in TNF dependent NF-kappa B activation are not understood ... Overall, our results indicate that intermediates required for NF-kappa B activation by TNF or ceramide are membrane bound, but the mechanism of activation by TNF is most likely different from that of ceramide
Satriano et al., J Clin Invest 1994 : Both PDTC and HMAP attenuated the increase in nuclear NF-kB in response to either TNF-alpha or IgG complexes ... Preincubation of cells with 8 br-cAMP, forskolin, or PGE2 attenuated the increase in nuclear NF-kB in response to TNF-alpha , aggregated IgG, or superoxide anion
McKean et al., J Exp Med 1994 : In this report we demonstrate that the transient nature of IL-1 induced NF-kappa B nuclear translocation occurs as a consequence of ligand induced receptor desensitization
Kanno et al., Mol Cell Biol 1994 : However, neither underlying mechanisms nor kinetics of the Tax mediated activation of NF-kappa B are understood ... The initial NF-kappa B binding activity seen after induction of Tax is due almost exclusively to p50/p65 heterodimers ... As nuclear NF-kappa B rapidly and potently stimulates resynthesis of I kappa B-alpha , the steady-state level of I kappa B-alpha does not significantly change
Perkins et al., Mol Cell Biol 1994 : We have previously shown that a cooperative interaction of NF-kappa B with Sp1 is required for the efficient stimulation of HIV-1 transcription
Sun et al., Mol Cell Biol 1994 : Human T-cell leukemia virus type I Tax activation of NF-kappa B/Rel involves phosphorylation and degradation of I kappa B alpha and RelA ( p65 ) -mediated induction of the c-rel gene ... HTLV-I Tax expression leads to the constitutive nuclear expression of biologically active NF-kappa B and c-Rel complexes ; however, the biochemical mechanism ( s ) underlying this response remains poorly understood ... In this study, we demonstrate that Tax stimulated nuclear expression of NF-kappa B in both HTLV-I infected and Tax transfected human T cells is associated with the phosphorylation and rapid proteolytic degradation of I kappa B alpha ... However, the pathologic action HTLV-I Tax leads to the deregulated and sustained nuclear expression of both NF-kappa B and c-Rel, a response that may contribute to HTLV-I induced T-cell transformation
Beauparlant et al., Oncogene 1994 (Cell Transformation, Neoplastic) : In contrast, overexpression of I kappa B alpha resulted in decreased saturation density, a flattened cellular morphology and decreased NF-kappa B dependent reporter gene activity
Ito et al., Nucleic Acids Res 1994 : Upon receiving various extra- and intracellular signals, I kappa B-alpha is rapidly degraded and NF-kappa B is induced to translocate into the nucleus
Tan et al., Biochim Biophys Acta 1994 : Our results indicate that both PAF and TNF stimulate the expression of NF-kappa B p50/p105 in vivo ... Our results indicate that both PAF and TNF stimulate the expression of NF-kappa B p50/p105 in vivo
Traenckner et al., EMBO J 1994 : Activation of the inducible transcription factor NF-kappa B involves removal of the inhibitory subunit I kappa B-alpha from a latent cytoplasmic complex ... Micromolar amounts of the peptide Cbz-Ile-Glu ( O-t-Bu ) -Ala-leucinal ( PSI ), a specific inhibitor of the chymotrypsin-like activity of the proteasome, prevented activation of NF-kappa B in response to tumor necrosis factor-alpha (TNF) and okadaic acid ( OA ) through inhibition of I kappa B-alpha degradation ... However, the covalent modification of I kappa B-alpha was not sufficient for activation of NF-kappa B : no substantial NF-kappa B DNA binding activity appeared in cells because the newly phosphorylated form of I kappa B-alpha was still tightly bound to p65 NF-kappa B ... This suggests that phosphorylation of I kappa B-alpha is equally necessary for the activation of NF-kappa B. ( ABSTRACT TRUNCATED AT 250 WORDS )
Jabado et al., Eur J Immunol 1994 : Interaction of HIV gp120 and anti-CD4 antibodies with the CD4 molecule on human CD4+ T cells inhibits the binding activity of NF-AT, NF-kappa B and AP-1, three nuclear factors regulating interleukin-2 gene enhancer activity ... Interaction of HIV gp120 and anti-CD4 antibodies with the CD4 molecule on human CD4+ T cells inhibits the binding activity of NF-AT, NF-kappa B and AP-1, three nuclear factors regulating interleukin-2 gene enhancer activity
Shimizu et al., Gene 1994 (Inflammation) : The results of mutational analysis on the SAA3 promoter indicate that both the NF-kappa B and C/EBP transcription factor binding motifs are essential for cytokine induced SAA3 gene expression in Hep3B cells
Suzuki et al., J Immunol 1994 : NF-kappa B activation induced by okadaic acid or calyculin A was not blocked by a myosin light chain kinase inhibitor, but was prevented by a protease inhibitor ... These results suggest that 1 ) phosphatase inhibitors either target a component of signal transduction, which occurs downstream to an antioxidant-sensitive step or use distinct signaling pathways ; 2 ) inhibition of phosphatases 1 and 2A is not a step in the pathway of TNF-alpha-, lymphotoxin-, or PMA induced NF-kappa B activation ; 3 ) myosin light chain kinase does not participate in NF-kappa B activation ; and 4 ) activation of NF-kappa B by phosphatase inhibitors is controlled by proteases
Kuno et al., J Leukoc Biol 1994 : Activation of both acid and neutral sphingomyelinases ( SMases ) has been suggested, and Schutz et al. proposed that the phosphatidylcholine-phospholipase C/acid SMase pathway is involved in TNF induced NF-kappa B activation ... However, our recent study showed that the NF-kappa B activation is induced by IL-1/TNF in fibroblasts from patients with type A Niemann-Pick disease, with acid SMase deficiency ... However, our recent study showed that the NF-kappa B activation is induced by IL-1/TNF in fibroblasts from patients with type A Niemann-Pick disease, with acid SMase deficiency ... This finding implies that acid SMase activity is not essential for the activation of NF-kappa B by IL-1/TNF at least in fibroblasts ... This finding implies that acid SMase activity is not essential for the activation of NF-kappa B by IL-1/TNF at least in fibroblasts
Muñoz et al., J Virol 1994 : Tax induces nuclear translocation of NF-kappa B through dissociation of cytoplasmic complexes containing p105 or p100 but does not induce degradation of I kappa B alpha/MAD3 ... Tax induces nuclear translocation of NF-kappa B through dissociation of cytoplasmic complexes containing p105 or p100 but does not induce degradation of I kappa B alpha/MAD3 ... Tax induces nuclear translocation of NF-kappa B through dissociation of cytoplasmic complexes containing p105 or p100 but does not induce degradation of I kappa B alpha/MAD3 ... These results suggest that Tax activates NF-kappa B essentially through the p105/p100 retention pathway
Kuno et al., Int Immunol 1994 (Niemann-Pick Diseases) : Furthermore, activation of NFkB was also induced in NPD type A fibroblasts in response to IL-1 alpha and TNF-alpha stimulation to a similar extent as in normal fibroblasts ... Furthermore, activation of NFkB was also induced in NPD type A fibroblasts in response to IL-1 alpha and TNF-alpha stimulation to a similar extent as in normal fibroblasts
Li et al., J Biol Chem 1994 : Phosphorylation of NF-KB1-p50 is involved in NF-kappa B activation and stable DNA binding
Chan et al., J Biol Chem 1994 (Lymphoma, Large B-Cell, Diffuse) : These results, overall, suggest that although TNF receptors are essential for induction of NF-kappa B , a small percentage is sufficient to fully transduce this signal
Finco et al., Proc Natl Acad Sci U S A 1994 : Due to the pleiotropic effects of these protease inhibitors, it is difficult to conclude from their use how I kappa B alpha phosphorylation and degradation contribute to NF-kappa B activation ... In the present study, a more direct approach demonstrates that phosphorylation of I kappa B alpha alone is not sufficient for NF-kappa B activation
Frankenberger et al., Biochem J 1994 : Protein-DNA complexes of constitutive NF-kappa B are similar in mobility to the LPS induced NF-kappa B and both are recognized by an antibody specific to the p50 subunit of NF-kappa B ... By contrast, treatment of cells with pyrrolidine dithiocarbamate ( PDTC ) will only block LPS induced NF-kappa B , but not the constitutive binding protein
Suzuki et al., Biochem Mol Biol Int 1994 (Leukemia, T-Cell) : Both nitecapone and OR-1246 ( 10-300 microM ) inhibited NF-kappa B activation induced by tumor necrosis factor-alpha in Jurkat T ( acute human leukemia ) cells
Cogswell et al., J Immunol 1994 : NF-kappa B regulates IL-1 beta transcription through a consensus NF-kappa B binding site and a nonconsensus CRE-like site
Jabbar et al., Leuk Res 1994 (Leukemia, B-Cell...) : In haemopoietic cell lines, TNF induction of NF-kappa B is mediated via the generation of reactive oxygen intermediates and by the activation of protein kinase C ( PKC ) ... Raising glutathione levels with N-acetyl cysteine substantially reduced NF-kappa B induction by TNF in two of four samples tested ... However, the protein kinase inhibitor staurosporine inhibited TNF induction of NF-kappa B in four of five samples, suggesting that staurosporine-sensitive protein kinases ( other than PKC ) are involved in the signalling pathway ... Our results suggest that PKC independent pathways, including pathways sensitive to redox reagents, mediate the induction of NF-kappa B by TNF in chronic B-leukaemia cells
Lee et al., J Biol Chem 1994 : Neither TPA nor IL-1 alpha induced NF-kappa B binding activity, as assessed by electrophoretic mobility shift analysis
McCaffrey et al., Nucleic Acids Res 1994 : Based on these results, we propose that the NF-kappa B site of the IL2R alpha promoter mediates at least part of the CsA sensitivity of IL2R alpha gene induction in untransformed T cells, possibly because de novo synthesis of p105 NF-kappa B is required for sustained IL2R alpha expression
Ng et al., J Biol Chem 1994 : Although tumor necrosis factor (TNF) and interleukin-1 ( IL ) induce the expression of the IL-8, TNF stimulated gene 6, and plasminogen activation inhibitor-2 genes and the activation of NF-kappa B with nearly identical kinetics, the two cytokines differ significantly in the induction of the IL-6 gene in all primary fibroblasts tested ... Although tumor necrosis factor (TNF) and interleukin-1 ( IL ) induce the expression of the IL-8, TNF stimulated gene 6, and plasminogen activation inhibitor-2 genes and the activation of NF-kappa B with nearly identical kinetics, the two cytokines differ significantly in the induction of the IL-6 gene in all primary fibroblasts tested ... Although tumor necrosis factor (TNF) and interleukin-1 ( IL ) induce the expression of the IL-8, TNF stimulated gene 6, and plasminogen activation inhibitor-2 genes and the activation of NF-kappa B with nearly identical kinetics, the two cytokines differ significantly in the induction of the IL-6 gene in all primary fibroblasts tested
Gruber et al., Nucleic Acids Res 1994 : These results indicate that apo CIII gene expression is regulated by NF-kappa B , and suggest that apo CIII production may be modulated by cellular signals, like inflammatory cytokines, that activate NF-kB
Jabbar et al., Br J Haematol 1994 (Leukemia, Hairy Cell...) : The induction of NF kappa B by TNF is mediated via a novel signalling pathway involving the generation of reactive oxidative intermediates ... Here we have used an electrophoretic mobility shift assay to show that TNF induced NF kappa B in malignant cells isolated from 3/3 HCL and 15/15 B-CLL patients
Bergmann et al., J Immunol 1994 : Unlike TNF-alpha mediated cytotoxicity, the TNF-alpha induced activation of NF-kappa B was inhibited by antioxidants regardless whether adenosine and homocysteine were present or absent in the culture medium ... In conclusion, a combination of adenosine and homocysteine selectively modulates TNF-alpha mediated cytotoxicity without changing the TNF-alpha induced activation of NF-kappa B
Wu et al., J Biol Chem 1994 : NF-kappa B activation of p53 ... In HeLa cells, tumor necrosis factor alpha (TNF-alpha) induced NF-kappa B activity ... Thus, we show that NF-kappa B activates p53 and that this activation is inducible by TNF-alpha ... Thus, we show that NF-kappa B activates p53 and that this activation is inducible by TNF-alpha ... Since NF-kappa B induction occurs as a response to stress and p53 arrests cells in G1/S, where repair may be initiated, activation of p53 by NF-kappa B could be a mechanism by which cells can recover from stress
Ueda et al., J Immunol 1994 : NF-kappa B and Sp1 regulate transcription of the human monocyte chemoattractant protein-1 gene
Wautier et al., Proc Natl Acad Sci U S A 1994 (Diabetes Complications...) : Binding of diabetic erythrocytes to endothelium generated an oxidant stress, as measured by production of thiobarbituric acid-reactive substances ( TBARS ) and activation of the transcription factor NF-kappa B , both of which were blocked by probucol or anti-RAGE IgG
Los et al., Biochem J 1994 : Whereas anti-CD28 together with PMA increased the DNA binding and trans-activation activity of NF-kappa B , PMA induced activation of AP-1 was significantly suppressed
Saldeen et al., Biochem Biophys Res Commun 1994 : Interleukin-1 beta induced activation of NF-kappa B in insulin producing RINm5F cells is prevented by the protease inhibitor N alpha-p-tosyl-L-lysine chloromethylketone ... Using the insulin producing rat cell line RINm5F and an electrophoretic mobility shift assay ( EMSA ), it was presently found that IL-1 beta induced a rapid activation ( 5 min ) of the transcription factor NF-kappa B and that this event was prevented by the protease inhibitor N alpha-p-tosyl-L-lysine chloromethylketone ( TLCK )
Swingler et al., Biochem Biophys Res Commun 1994 (Astrocytoma...) : In human astrocytoma and neuroblastoma cell lines tumour necrosis factor alpha (TNF alpha) and interleukin 1 beta (IL-1 beta) induced NFKB and an additional KB-specific protein of approximately 80 K to bind the HIV-1 enhancer ... In human astrocytoma and neuroblastoma cell lines tumour necrosis factor alpha (TNF alpha) and interleukin 1 beta (IL-1 beta) induced NFKB and an additional KB-specific protein of approximately 80 K to bind the HIV-1 enhancer
Albrecht et al., FEBS Lett 1994 : Bcl-2 protects from oxidative damage and apoptotic cell death without interfering with activation of NF-kappa B by TNF ... However, Bcl-2 had no effect on the activation of NF-kappa B by TNF , even though it protected cells from TNF induced apoptosis
Adcock et al., Life Sci 1994 : The interaction of the transcription factors, activator protein 1 (AP-1) and nuclear factor kappa from B cells ( NF kappa B ) with DNA and glucocorticoid receptors was analysed by gel mobility shift assays following stimulation by tumour necrosis factor alpha (TNF alpha) and a phorbol ester ( PMA ) that activates protein kinase C. PMA and TNF alpha both caused significant ( 180-340 % ) increases in AP-1 and NF kappa B DNA binding which peaked at 15 minutes and decreased to a constant elevated level at between 1-3 hrs and was sustained for 24hrs
Sun et al., Science 1993 : NF-kappa B controls expression of inhibitor I kappa B alpha : evidence for an inducible autoregulatory pathway ... Phorbol ester and tumor necrosis factor-alpha induction of nuclear NF-kappa B is associated with both the degradation of performed I kappa B alpha and the activation of I kappa B alpha gene expression ... Association of the newly synthesized I kappa B alpha with p65 restores intracellular inhibition of NF-kappa B DNA binding activity and prolongs the survival of this labile inhibitor ... Association of the newly synthesized I kappa B alpha with p65 restores intracellular inhibition of NF-kappa B DNA binding activity and prolongs the survival of this labile inhibitor ... Together, these results show that NF-kappa B controls the expression of I kappa B alpha by means of an inducible autoregulatory pathway
Costello et al., Cell Growth Differ 1993 : Increase of IL-2R alpha gene transcription involves the enhanced binding of the transcription factor NF-kappa B to its consensus sequence in the 5'-regulatory region of the IL-2R alpha gene
Eck et al., Mol Cell Biol 1993 : To investigate the role of NF-kappa B in cell adhesion molecule expression, we treated HL-60 cells with a double stranded oligonucleotide which specifically inhibits NF-kappa B-mediated transcription
Frantz et al., EMBO J 1994 : Calcineurin acts in synergy with PMA to inactivate I kappa B/MAD3 , an inhibitor of NF-kappa B
Laegreid et al., J Biol Chem 1994 : It was found that cross linking of TNFR p75 led to strong induction of the human cytomegalovirus enhancer as well as activation of nuclear factor-kappa B (NF-kappa B) ... Stimulation of TNFR p75 also mediated activation of NF-kappa B in human KYM-1 rhabdomyosarcoma cells but not in other cell types such as U937 and HL-60 monocytic cells or in Eahy 926 endothelial cells ... NF-kappa B activation induced by TNFR p75 was delayed approximately 15 min compared with NF-kappa B activation induced by TNFR p55, indicating that the two TNFRs activate NF-kappa B through different signaling pathways ... NF-kappa B activation induced by TNFR p75 was delayed approximately 15 min compared with NF-kappa B activation induced by TNFR p55 , indicating that the two TNFRs activate NF-kappa B through different signaling pathways ... The data presented in this study identify intracellular responses mediated by TNFR p75 which have not been reported previously and suggest that TNFR p75 induced activation of NF-kappa B is strictly cell type-specific
Schenk et al., Proc Natl Acad Sci U S A 1994 : These results suggest that thioredoxin plays an important role in the regulation of transcriptional processes and oppositely affects NF-kappa B and AP-1 activation
Betts et al., J Biol Chem 1994 (Leukemia, Promyelocytic, Acute...) : The participation of cell ceramide in tumor necrosis factor (TNF)-alpha stimulated NF-kappa B activation in Jurkat T cells and HL-60 cells was studied ... TNF-alpha readily stimulated NF-kappa B activity in both cell lines as assayed by electrophoretic mobility shift assay and the use of a human immunodeficiency virus-chloramphenicol acetyltransferase reporter construct ... We conclude that TNF-alpha induced NF-kappa B activation occurs in Jurkat and HL-60 cell lines that do not demonstrate an increase in TNF-alpha induced ceramide ... Increasing ceramide levels by the addition of short chain ceramides or the use of a glucosylceramide synthase inhibitor can be dissociated from activation of NF-kappa B by TNF-alpha
Zhang et al., Proc Natl Acad Sci U S A 1994 : Activation of the interleukin 6 gene by Mycobacterium tuberculosis or lipopolysaccharide is mediated by nuclear factors NF-IL6 and NF-kappa B
Iwasaki et al., Cytokine 1993 : In both cell lines, IL-1 alpha activated NF-kappa B-like DNA binding activity but in EL-4 6.1 C10 cells the IL-1 alpha concentration sufficient to induce NF-kappa B response was 1000-fold lower than in 70Z/3 cells
Yan et al., J Biol Chem 1994 (Diabetes Mellitus) : AGE albumin or AGEs immunoisolated from diabetic plasma resulted in induction of endothelial cell ( EC ) oxidant stress, including the generation of thiobarbituric acid reactive substances ( TBARS ) and resulted in the activation of NF-kappa B , each of which was blocked by antibodies to AGE receptor polypeptides and by antioxidants
Lacoste et al., Leukemia 1994 : NF-kappa B proteins differentially trans activated the HIV-1 enhancer-CAT reporter ; co-expression of Tax abrogated the inhibitory effect of I kappa B alpha and a trans-dominant negative mutant of p65 ( p65 delta ), indicating that Tax was a trans-dominant activator of NF-kappa B-regulated genes
Beg et al., Oncogene 1994 : Activation of multiple NF-kappa B/Rel DNA binding complexes by tumor necrosis factor
Miyamoto et al., Mol Cell Biol 1994 : Enhanced I kappa B alpha degradation is responsible for constitutive NF-kappa B activity in mature murine B-cell lines ... Rapid degradation of I kappa B alpha is directly involved in constitutive NF-kappa B activation, because stabilization of I kappa B alpha by a protease inhibitor causes loss of NF-kappa B activity in mature B cells ... These results provide evidence that continuous and rapid degradation of I kappa B alpha in the absence pf external stimuli is causally involved in the constitutive activation of NF-kappa B in mature murine B cells
Chaturvedi et al., J Biol Chem 1994 (Fibrosarcoma...) : By using receptor blocking antibodies we found that both p60 and p80 forms of TNF receptors were functional for NBT reducing activity, but TNF dependent NF-kappa B activation required only the p60 receptor ... By using receptor blocking antibodies we found that both p60 and p80 forms of TNF receptors were functional for NBT reducing activity, but TNF dependent NF-kappa B activation required only the p60 receptor
Machleidt et al., J Biol Chem 1994 : Various inhibitors of serine-like proteases are shown to block TNF mediated NF-kappa B activation as well as the disappearance of I kappa B-alpha immunoreactivity in primary murine T lymphocytes and in various human leukemic cell lines
Zhang et al., Mol Cell Biol 1994 (Leukemia, Lymphocytic, Chronic, B-Cell...) : In contrast to I kappa B alpha ( MAD3 ), BCL3 does not cause NF-kappa B p50 to be retained in the cytoplasm ; instead, in cotransfection assays, it alters the subnuclear localization of p50
Meyer et al., Nucleic Acids Res 1994 : While the LPS induction of the kappa intron enhancer is mediated by NF-kappa B , this factor is not responsible for activation of the 3 ' enhancer
Batuman et al., Immunopharmacology 1994 : The data indicate that 10 ( -6 ) M DM increased T cell p50 NF-kappa B mRNA levels by four-fold compared to the levels obtained in the absence of DM
Pimentel-Muiños et al., J Immunol 1994 : Interestingly, a neutralizing anti-TNF-alpha mAb strongly inhibited the proliferation, IL-2R alpha expression, and activation of the nuclear factor (NF)-kappa B induced on human purified T lymphocytes by an immobilized anti-CD3 mAb ( imm.anti-CD3 ) ... Interestingly, a neutralizing anti-TNF-alpha mAb strongly inhibited the proliferation, IL-2R alpha expression, and activation of the nuclear factor (NF)-kappa B induced on human purified T lymphocytes by an immobilized anti-CD3 mAb ( imm.anti-CD3 ) ... Cyclosporin A, which prevented lymphokine mRNA transcription, inhibited more than 90 % of the IL-2R alpha and NF-kappa B levels induced by TCR/CD3 mediated activation ... However, only TNF-alpha ( but not IL-2 ) efficiently recovered the nuclear NF-kappa B levels
Johns et al., J Immunol 1994 : Recent studies demonstrate that the sphingomyelinase-ceramide pathway plays a potential role in the activation of nuclear factor-kappa B (NF-kappa B) by TNF-alpha ... This data suggests that although ceramide and 1,2-diacylglycerol ( DAG ) pathways may contribute to TNF-alpha activation of NF-kappa B , impedance of these pathways does not block TNF-alpha from activating NF-kappa B nor induction of the functional activation of the NF-kappa B responsive reporter construct, HCMV
Rice et al., EMBO J 1993 : In vivo control of NF-kappa B activation by I kappa B alpha
Sato et al., J Biol Chem 1993 : Binding sites for NF-kB , Sp-1, and AP-1 are reportedly required for induction of MMP-9 gene expression by tumor necrosis factor-alpha or 12-O-tetradecanoylphorbol-13-acetate
Donnelly et al., J Immunol 1993 : Because NF-kappa B activation is required for transcription of many cytokine genes, including IL-6 , the ability of IL-4 to suppress NF-kappa B activity in monocytes suggests a potential mechanism by which this molecule may inhibit the expression of multiple cytokines
Betts et al., J Biol Chem 1993 : The role of NF-kappa B and NF-IL6 transactivating factors in the synergistic activation of human serum amyloid A gene expression by interleukin-1 and interleukin-6
Gross et al., J Clin Immunol 1993 (Carcinoma, Squamous Cell...) : In contrast, NF-kB was increased by both IL-1 and PMA, but only the effect of PMA, and not that of IL-1, was inhibited by staurosporine
Biswas et al., Proc Natl Acad Sci U S A 1993 : A cooperative action of PTX ( NF-kappa B inhibitor ) and Ro 24-7429 ( Tat inhibitor ) on HIV-1 LTR regulated gene expression is suggested
Menon et al., J Biol Chem 1993 : Inability to induce NF-kappa B can not be due to a non-activatable system since NF-kappa B was strongly activated by tumor necrosis factor in all the five primary cell types tested ... Conversely cysteine, an antioxidant and precursor of the free radical scavenger, glutathione, inhibited the induction of NF-kappa B by tumor necrosis factor in primary cells, and by okadaic acid or tumor necrosis factor in transformed cells ... The induction of NF-kappa B by tumor necrosis factor in primary cells suggests that this cytokine fulfills the requirement for oxidation, possibly by inducing the production of free radicals
Geng et al., J Immunol 1993 : Both PTK inhibitors also reduced the LPS activation of nuclear factor-kappa B (NF-kappa B) , which is a transcription factor involved in the expression of cytokine genes such as IL-6 and TNF-alpha ... Both PTK inhibitors also reduced the LPS activation of nuclear factor-kappa B (NF-kappa B) , which is a transcription factor involved in the expression of cytokine genes such as IL-6 and TNF-alpha ... In summary, these findings suggest that protein kinase C and protein kinase A appear to have selective effects in the LPS induction of cytokines, whereas PTK is required for LPS induction of a broad spectrum of cytokines and NF-kappa B activation in monocytes
Lander et al., J Immunol 1993 : In support of the existence of this pathway in whole cells, we found that the G protein inhibitor, GDP-beta-S, blocked NF-kappa B translocation induced by nitric oxide or LPS in permeabilized cells
Shattuck et al., Mol Cell Biol 1994 (Melanoma) : The effect of deletion or mutation of the MGSA/GRO alpha NF-kappa B element, combined with data from gel mobility shift analyses, indicates that the NF-kappa B p50/p65 heterodimer in RPE cells plays an important role in IL-1- and TNF alpha enhanced gene transcription ... In Hs294T cells, gel shift analyses indicate that IL-1 and TNF alpha induce NF-kappa B complex formation ; however, transactivation does not occur, suggesting that subtle differences in the NF-kappa B complexes may result in the inability of the cytokines IL-1 and TNF alpha to activate transcription of the MGSA/GRO genes ... In Hs294T cells, gel shift analyses indicate that IL-1 and TNF alpha induce NF-kappa B complex formation ; however, transactivation does not occur, suggesting that subtle differences in the NF-kappa B complexes may result in the inability of the cytokines IL-1 and TNF alpha to activate transcription of the MGSA/GRO genes
Rodríguez et al., Immunogenetics 1994 (Breast Neoplasms...) : In addition, induction of NF-kB binding activity by the tumor necrosis factor was accompanied by a decrease in nuclear factors that bind to the CRE II region
Chiao et al., Proc Natl Acad Sci U S A 1994 : These data suggest the existence of an autoregulatory loop whereby I kappa B alpha regulates the activity of transcription factor NF-kappa B , which in turn regulates the I kappa B alpha activity
Wolchok et al., J Leukoc Biol 1994 (Plasmacytoma) : Both TNF and LPS activated the same NF-kappa B nuclear complexes, composed of the p50 and p65 subunits ... Both TNF and LPS activated the same NF-kappa B nuclear complexes, composed of the p50 and p65 subunits ... LPS mediated a stronger and more sustained activation of NF-kappa B than TNF ... However, PDTC had a smaller inhibitory effect on LPS induced NF-kappa B activation and H-2 antigen expression, suggesting that TNF and LPS activate NF-kappa B by somewhat different pathways ... However, PDTC had a smaller inhibitory effect on LPS induced NF-kappa B activation and H-2 antigen expression, suggesting that TNF and LPS activate NF-kappa B by somewhat different pathways
Béraud et al., Mol Cell Biol 1994 (Leukemia, T-Cell) : Human T-cell leukemia virus type I Tax associates with and is negatively regulated by the NF-kappa B2 p100 gene product : implications for viral latency
Leung et al., J Biol Chem 1994 (Leukemia, T-Cell) : The C-terminal region of the type I IL-1R is required for this effect, and deletion of its cytoplasmic domain abrogates the activation of NF-kappa B by IL-1
Suzuki et al., Biochem Mol Biol Int 1993 : Vitamin E derivatives have been shown to inhibit tumor necrosis factor induced NF-kappa B activation in human Jurkat T cells
Ray et al., Agents Actions 1993 : Activation of the transcription factor NF kappa B by the proinflammatory cytokine interleukin-1 (IL-1) has been investigated in the human lung epithelial cell line ( A549 ) ... Activation of NF kappa B by IL-1 ( 1 pM ) was prevented when incubations were performed in the presence of the IL-1 receptor antagonist protein ( 1 nM ) ... The results suggest that a novel kinase pathway may be involved in the activation of NF kappa B by IL-1 in these cells
de Martin et al., EMBO J 1993 : We describe here that in porcine aortic endothelial cells, either IL-1 alpha , TNF alpha or LPS upregulates an inhibitor of NF kappa B which we refer to as ECI-6 ... We describe here that in porcine aortic endothelial cells, either IL-1 alpha, TNF alpha or LPS upregulates an inhibitor of NF kappa B which we refer to as ECI-6
Diaz-Meco et al., Mol Cell Biol 1993 : It has recently been demonstrated in the model system of Xenopus laevis oocytes that the PMA-insensitive PKC isotype, zeta PKC, is a required step in the activation of NF-kappa B in response to ras p21
Naumann et al., Oncogene 1993 : NF-kappa B precursor p100 inhibits nuclear translocation and DNA binding of NF-kappa B/rel-factors
Schulze-Osthoff et al., EMBO J 1993 : This depletion of the mitochondrial oxidative metabolism resulted in resistance towards TNF cytotoxicity, as well as in inhibition of NF kappa B activation and interleukin-6 gene induction by TNF ... This depletion of the mitochondrial oxidative metabolism resulted in resistance towards TNF cytotoxicity, as well as in inhibition of NF kappa B activation and interleukin-6 gene induction by TNF
Dbaibo et al., J Biol Chem 1993 (Leukemia-Lymphoma, Adult T-Cell) : In this cell line, TNF-alpha caused potent activation of nuclear factor-kappa B (NF-kappa B) and exerted potent cytostatic/cytocidal activity ... C2-ceramide, however, enhanced activation of NF-kappa B in response to TNF-alpha with peak effects observed at a concentration of C2-ceramide of 5 microM
Henkel et al., Nature 1993 : Treatment of cells with various protease inhibitors or an antioxidant completely prevented the inducible decay of I kappa B-alpha as well as the activation of NF-kappa B ... In intact cells, phosphorylation of I kappa B-alpha is apparently not sufficient for activation of NF-kappa B
Yang et al., J Biol Chem 1993 (Leukemia, Promyelocytic, Acute) : As shown previously, TNF ( 1 nM ) induced a marked increase in nuclear NF-kappa B binding in human leukemia ( HL-60 ) cells within 5 min, and elevated binding was detected for as long as 1 h. Addition of a maximally effective concentration of sphingomyelinase, 0.1 units.ml-1, induced a 50 % reduction in sphingomyelin content by 5 min from a basal level of 560 pmol.10 ( 6 ) cells-1 and a quantitative increase in ceramide levels from 89 pmol.10 ( 6 ) cells-1
McGuire et al., J Virol 1993 : Several studies have shown that tax transactivation of the IL-2R alpha-chain promoter is mediated by binding sites for the transcriptional activator NF-kappa B , and this mechanism has also been implicated in the tax activation of IL-2 promoter activity
Muroi et al., Cell Signal 1993 : The electrophoretic mobility shift assays ( EMSA ) with the use of the synthetic HIV-1 NF-kappa B motif as a probe, showed that LPS-treatment of J774 cells ( a mouse macrophage cell line ) leads to the activation of the fast moving ( denoted as B1 ) and the slow moving NF-kappa B ( denoted as B2 ) ... LPS induced activation of NF-kappa B was inhibited by a protein kinase A (PKA) inhibitor ( H-89 ), but not by a protein kinase C ( PKC ) inhibitor ( H-7 ) ... LPS induced activation of NF-kappa B was inhibited by a protein kinase A (PKA) inhibitor ( H-89 ), but not by a protein kinase C ( PKC ) inhibitor ( H-7 ) ... PMA itself failed to activate NF-kappa B and the depletion of PKC did not prevent LPS induced activation of NF-kappa B ... Thus, these data suggested a probable involvement of PKA in LPS induced NF-kappa B activation in macrophages ... Thus, these data suggested a probable involvement of PKA in LPS induced NF-kappa B activation in macrophages
Paliogianni et al., J Immunol 1993 : IL-2 dependent IL-2R alpha expression in PMA blasts and NF-kB induction in resting human T cells were also inhibited by Dex
Arenzana-Seisdedos et al., J Virol 1993 : Induction of NF-kappa B by PC-PLC did not depend on de novo synthesis of proteins or autocrine secretion of either tumor necrosis factor or interleukin 1 ... In human monocytic and lymphoblastoid T-cell lines, induction of NF-kappa B by PC-PLC resulted in clear induction of luciferase expression vectors placed under the control of synthetic kappa B enhancers or wild type, but not kappa B-mutated, HIV long terminal repeat constructs ... PC-PLC induced NF-kappa B activation could not be blocked by a specific inhibitor of phorbol ester-inducible protein kinases C
Watanabe et al., Oncogene 1993 : HTLV-I encoded Tax in association with NF-kappa B precursor p105 enhances nuclear localization of NF-kappa B p50 and p65 in transfected cells
Dominguez et al., Mol Cell Biol 1993 : In this report, we present evidence that in oocytes from Xenopus laevis, ( i ) ras p21- and phospholipase C (PLC) mediated phosphatidylcholine ( PC ) hydrolysis activates NF-kappa B and ( ii ) protein kinase C zeta subspecies is involved in the activation of NF-kappa B in response to insulin/ras p21/PC-PLC ... In this report, we present evidence that in oocytes from Xenopus laevis, ( i ) ras p21- and phospholipase C (PLC) mediated phosphatidylcholine ( PC ) hydrolysis activates NF-kappa B and ( ii ) protein kinase C zeta subspecies is involved in the activation of NF-kappa B in response to insulin/ras p21/PC-PLC
Doerre et al., Proc Natl Acad Sci U S A 1993 : The c-rel protooncogene product represses NF-kappa B p65 mediated transcriptional activation of the long terminal repeat of type 1 human immunodeficiency virus
Lander et al., J Immunol 1993 : In addition, NF-kappa B binding activity was induced by these agents as was the secretion of TNF-alpha
Morris et al., J Exp Med 1993 : There was no induction of nuclear NF-kappa B in B-1 cells stimulated by sIgM crosslinking, although NF-kappa B was stimulated by phorbol myristate acetate and by LPS
Brach et al., J Biol Chem 1993 : Analysis of deleted fragments of the IL-6 promoter indicated that transcriptional activation of the IL-6 promoter was due to enhanced binding activity of the transcription factor nuclear factor (NF)-kappa B
Kalvakolanu et al., Proc Natl Acad Sci U S A 1993 (Teratoma) : We have recently shown that the adenovirus E1A gene products block interferon-alpha induced signal transduction and transcription factor NF-kappa B-mediated gene induction ... We have recently shown that the adenovirus E1A gene products block interferon-alpha induced signal transduction and transcription factor NF-kappa B-mediated gene induction
Meyer et al., EMBO J 1993 : H2O2 and antioxidants have opposite effects on activation of NF-kappa B and AP-1 in intact cells : AP-1 as secondary antioxidant-responsive factor
Beyaert et al., Cancer Res 1993 (Fibrosarcoma...) : At the biochemical level, staurosporine increased the TNF mediated activation of phospholipases C and D and the transcription factor NF-kappa B in L929 cells
Hayashi et al., J Biol Chem 1993 : We show that a cellular reducing catalyst thioredoxin (Trx) plays a major role in activation of the DNA binding of NF kappa B in vitro and stimulation of transcription from the NF kappa B-dependent gene expression ... We demonstrate evidence suggesting that redox regulation of NF kappa B by Trx might be exerted at a step after dissociation of the inhibitory molecule I kappa B, a cytosolic anchoring protein for NF kappa B
Nolan et al., Mol Cell Biol 1993 : At concentrations that significantly inhibited DNA binding by homodimeric p50, Bcl-3 did not inhibit binding of reconstituted heterodimeric NF-kappa B ( p50 : p65 ), a DNA binding homodimeric form of p65, or homodimers of c-Rel
Staal et al., AIDS Res Hum Retroviruses 1993 : We show ( 1 ) that N-acetyl-L-cysteine (NAC) , an antioxidant and an efficient glutathione ( GSH ) precursor, inhibits NF-kappa B activation and HIV expression under conditions in which GSH is depleted and NAC can not be converted to GSH, ( 2 ) that the D-stereoisomer of NAC and a wide variety of chemically unrelated antioxidants also inhibit NF-kappa B activation and/or transcription directed by the HIV LTR, and ( 3 ) that depletion of GSH, the principal intracellular antioxidant, augments HIV production in an acute infection model
Brach et al., J Immunol 1993 : Transcriptional activation of the macrophage colony stimulating factor gene by IL-2 is associated with secretion of bioactive macrophage colony stimulating factor protein by monocytes and involves activation of the transcription factor NF-kappa B ... Transcriptional activation of the macrophage colony stimulating factor gene by IL-2 is associated with secretion of bioactive macrophage colony stimulating factor protein by monocytes and involves activation of the transcription factor NF-kappa B
Trede et al., J Immunol 1993 (Leukemia, Monocytic, Acute) : Induction of both NF-kappa B DNA binding proteins and NF-kappa B enhancer function was down-regulated by inhibitors of protein kinase C and protein tyrosine kinase, indicating a role for these protein kinases in the induction of NF-kappa B by MHC class II ligands ... Induction of both NF-kappa B DNA binding proteins and NF-kappa B enhancer function was down-regulated by inhibitors of protein kinase C and protein tyrosine kinase , indicating a role for these protein kinases in the induction of NF-kappa B by MHC class II ligands
Raes et al., C R Seances Soc Biol Fil 1995 (Second Messenger Systems) : However several questions regarding the molecular mechanisms involved in particular steps of these cascades remain largely unresolved : how and at which subcellular level, do the cells produce these reactive oxygen intermediates that will contribute to NF kappa B activation in response to IL-1 or TNF-alpha ? ... However several questions regarding the molecular mechanisms involved in particular steps of these cascades remain largely unresolved : how and at which subcellular level, do the cells produce these reactive oxygen intermediates that will contribute to NF kappa B activation in response to IL-1 or TNF-alpha ?
Fujimoto et al., Gene 1995 : p105 also functions as a cytoplasmic inhibitor for NF-kappa B, and the proteolytic processing of its inhibitory C-terminal region is required for generation of active NF-kappa B
Cohen et al., Immunobiology 1995 : The LPS or IL-1 induced NF-kappa B binding activity was increased in the presence of MDP but not of MDP ( D, D ) ... The LPS or IL-1 induced NF-kappa B binding activity was increased in the presence of MDP but not of MDP ( D, D ) ... A mutant of the cell line called 1.3E2, defective in NF-kappa B activations by LPS , did not respond to MDP
Lin et al., J Cell Biol 1995 (Neuroblastoma...) : Understanding the precise mechanism by which Bcl-2 and thiol agents inhibit SV-induced nuclear NF-kappa B activity in AT-3 cells may provide insights into the pluripotent antiapoptotic actions of these agents
Granowitz et al., Mol Med 1995 (HIV Seropositivity...) : The effects of IL-1 and IL-1Ra on nuclear factor-kappa B (NF-kappa B) DNA binding activity, as measured by electrophoretic mobility shift assays, were also determined ... The effects of IL-1 and IL-1Ra on nuclear factor-kappa B (NF-kappa B) DNA binding activity, as measured by electrophoretic mobility shift assays, were also determined ... IL-1 increased NF-kappa B DNA binding activity and IL-1Ra blocked this effect ... IL-1 increased NF-kappa B DNA binding activity and IL-1Ra blocked this effect ... IL-1 induces HIV-1 expression ( via the IL-1RI ) and NF-kappa B activity in U1 cells
Munoz et al., Immunobiology 1995 : These results suggest that Tax activates NF-kappa B essentially through the p105/p100-retention pathway
Schütze et al., Immunobiology 1995 : TNF induced activation of NF-kappa B ... Besides endosomal acidic SMase, TNF also rapidly activates a plasmamembrane associated neural SMase (nSMase), that, however is not involved in TNF induced NF-kappa B activation
Jahnke et al., Immunobiology 1995 (Melanoma) : Using electrophoretic mobility shift assays and specific antibodies we observed that the NF-kappa B like sequence at -187 bound both p65/p50 and p65/c-Rel in the presence of TNF-alpha , while the interferon responsive region at -75 bound Stat1 alpha ( p91 )
Wood et al., J Biol Chem 1995 (Melanoma) : Constitutive and cytokine induced expression of the melanoma growth stimulatory activity/GRO alpha gene requires both NF-kappa B and novel constitutive factors
Yamamoto et al., J Biol Chem 1995 : These data suggested a potential role of both NF-IL6 and NF kappa B in the induction of cyclooxygenase-2 by TNF alpha
Finnegan et al., J Immunol 1996 (Lymphoma, Large B-Cell, Diffuse) : In combination with TNF-alpha, IL-10 stimulated activating protein-1 (AP-1) and nuclear factor (NF)-kappa B binding activities and cooperated to increase HIV-1 steady-state mRNA levels and enhance long terminal repeat directed transcription through activation of the NF-kappa B binding sites, suggesting the IL-10 effect occurs at least in part at the transcriptional level
Lemasson et al., J Immunol 1996 : The Ig CDR3-like region-specific mAb, 13B8-2, blocked HIV-1 production in CD4-positive/p56Ick negative HTLV-I producing MT2 cells superinfected by HIV-1Lai, but had no effect on HTLV-I production, although it did inhibit Tax induced NF-kappa B translocation
Bitar et al., Cell Growth Differ 1995 : Interestingly, expression of RelA ( p65 ), c-Rel, NF-kappa B1 ( p105 ), NF-kappa B2 ( p100 ), and NF-kappa B2 ( p52 ) subunits increased I kappa B alpha protein levels from 3- to 30-fold, indicating that one mechanism to compensate for the increased expression of NF-kappa B proto-oncogenes was to increase the synthesis and/or stability of the regulatory I kappa B alpha protein
Baldi et al., J Biol Chem 1996 : NF-kappa B is readily activated from normally inhibited cytoplasmic stores by induced proteolytic degradation of I kappa B-alpha , a principal inhibitor of this transcription factor
Drew et al., J Neuroimmunol 1995 (Neuroblastoma) : Interferon regulatory factor-2 physically interacts with NF-kappa B in vitro and inhibits NF-kappa B induction of major histocompatibility class I and beta 2-microglobulin gene expression in transfected human neuroblastoma cells
Hallahan et al., Biochem Biophys Res Commun 1995 : These data demonstrate that E-selectin expression does not require cytokine synthesis, but involves NFkB activation
Sappey et al., AIDS Res Hum Retroviruses 1995 : DFO also protected against PMA induced NF-kappa B activation as well as TNF-alpha induced HIV-1 activation
Corsini et al., Photodermatol Photoimmunol Photomed 1995 : TPCK, a I kappa-B alpha protease inhibitor, was able to virtually abolish UV-induced TNF release, indicating that UV-induced TNF release requires NF-kappa B activation
Callaghan et al., J Leukoc Biol 1996 (Leukemia, Myelomonocytic, Acute) : Additionally, TNF-alpha induced PTK and NF-kappa B activation were important in collagenase gene expression in this cell line
Chauhan et al., Blood 1996 (Multiple Myeloma) : Multiple myeloma cell adhesion induced interleukin-6 expression in bone marrow stromal cells involves activation of NF-kappa B
Zhou et al., Eur J Immunol 1996 : Since it has been documented that the expression of E-selectin and Mac-1 is regulated either directly or indirectly by the transcription factor NF kappa B , our studies provide in vivo evidence that tepoxalin is a potent inhibitor of NF kappa B-mediated events in animal models and this novel molecular mechanism clearly defines it as a new class of anti-inflammatory compounds
Rovin et al., Kidney Int 1995 : This study investigated the role of NF-kappa B in IL-1 induced MCP-1 expression by human mesangial cells ... These data suggest that MCP-1 gene transcription may be mediated , in part, by the transcription factor NF-kappa B
Tetsuka et al., Biochem Biophys Res Commun 1996 : Tyrosine kinase inhibitors, genistein and herbimycin A, do not block interleukin-1 beta induced activation of NF-kappa B in rat mesangial cells ... Since both COX-2 and iNOS promoters have a kappa B binding motif, we have evaluated the effects of tyrosine kinase inhibitors on IL-1 beta induced nuclear factor-kappa B (NF-kappa B) activation by electromobility shift assays ... IL-1 beta rapidly induced the translocation of NF-kappa B in rat mesangial cells ... These data suggest that an upstream tyrosine kinase pathway may not be required for IL-1 beta induced NF-kappa B activation and that the tyrosine kinase pathway may converge with the NF-kappa B pathway down-stream of NF-kappa B activation in rat mesangial cells
Jabbar et al., Leukemia & lymphoma 1995 (Leukemia, Hairy Cell...) : TNF induction of NF-kappa B was abolished by antioxidants, suggesting involvement of the free radical pathway
Rao et al., J Interferon Cytokine Res 1995 : Upregulation of NF-kappa B-dependent gene expression mediated by the p75 tumor necrosis factor receptor ... Upregulation of NF-kappa B-dependent gene expression mediated by the p75 tumor necrosis factor receptor
Rensing-Ehl et al., J Inflamm 1995 : With regard to their cytoplasmic homology region, we investigated whether Fas like the TNF-R activates nuclear factor kappa B (NF-kappa B) , using human SV80 fibroblasts transfected with the cDNA encoding human Fas ... Furthermore, when equitoxic concentrations of anti-Fas antibody and TNF were applied, TNF triggered a stronger NF-kappa B response
Lederer et al., J Immunol 1996 : We demonstrate that IL-1 and TNF induce rapid nuclear translocation of p65(RelA) in T cell clones, whereas TCR induced NF-Kappa B activation in Th1 cells is delayed and may be longer in duration ... We demonstrate that IL-1 and TNF induce rapid nuclear translocation of p65(RelA) in T cell clones, whereas TCR induced NF-Kappa B activation in Th1 cells is delayed and may be longer in duration ... We demonstrate that IL-1 and TNF induce rapid nuclear translocation of p65(RelA) in T cell clones, whereas TCR induced NF-Kappa B activation in Th1 cells is delayed and may be longer in duration
Chen et al., Cell 1996 : Signal induced activation of the transcription factor NF-kappaB requires specific phosphorylation of the inhibitor IkappaBalpha and its subsequent proteolytic degradation
Hecker et al., FEBS Lett 1996 : DCI , but not NAS, also suppressed the activation of NF-kappa B in VSMC exposed to IL-1 beta, while no significant NF-kappa B activation was detected in forskolin treated cells ... They also suggest that reactive oxygen intermediates do not play a role in the activation of NF-kappa B by IL-1 beta in VSMC, and that transcription factors other than NF-kappa B mediate the induction of iNOS expression by elevating the intracellular concentration of cyclic AMP
Menegazzi et al., FEBS Lett 1996 : These results suggest the likelihood of an ordered sequence of events by which an activated NF-kappa B mediates the induction of iNOS gene expression in TPA- and/or CHX treated primary hepatocytes
Chen et al., Mol Immunol 1996 : The activity of the transcription factor NF-kappaB is tightly regulated by the inhibitory molecule IkappaBalpha ... In this study, we have mutated three potential phosphorylation sites in porcine IkappaBalpha and found that expression of the Ser32 mutant of IkappaBalpha ( IS32A ), but not Tyr42 or Ser262 mutants or wild-type IkappaBalpha, blocked the activation of NF-kappaB by TNF-alpha
Mori et al., Blood 1996 (Bone Resorption...) : Moreover, deletion of either 5 ' or 3 ' NF-kappaB sites reduced IL-1alpha promoter activity in MT-2 cells and transactivation of the IL-1alpha promoter by exogenous NF-kappaB and Tax in Jurkat cells
Cobb et al., FEBS Lett 1996 : In this report, we investigated the effects of a 3-alkoxybenzo [ beta ] thiophene-2-carboxamide ( BZT ) on the cytokine induction of VCAM-1 expression and activation of the transcription factor NF-kappa B in human endothelial cells
Cheng et al., Genes Dev 1996 : TANK , a co-inducer with TRAF2 of TNF- and CD 40L mediated NF-kappaB activation ... TANK, a co-inducer with TRAF2 of TNF- and CD 40L mediated NF-kappaB activation ... TANK, a co-inducer with TRAF2 of TNF- and CD 40L mediated NF-kappaB activation ... In cotransfection experiments, TANK and TRAF2 activate NF-kappaB synergistically, requiring both the amino-terminal portion of TANK and the ring finger domain of TRAF2
Essani et al., J Immunol 1996 : However, the fact that hepatic parenchymal cells, despite NF-kappa B activation do not express E- selectin mRNA, suggests that NF-kappa B activation alone is not sufficient for E-selectin gene transcription in vivo
Hsu et al., Immunity 1996 : The death domain of tumor necrosis factor (TNF) receptor-1 ( TNFR1 ) triggers distinct signaling pathways leading to apoptosis and NF-kappa B activation through its interaction with the death domain protein TRADD
Claudio et al., Exp Cell Res 1996 (Fibrosarcoma) : TNFalpha induces a rapid and transient activation of NF-kappaB in WEHI 164 cells which is followed by a second, long lasting phase in which the amount of NF-kappaB complex in the nucleus remains at about 50 % of maximum ... Calphostin C, on the other hand, can block the activation of NF-kappaB by TNFalpha , also blocking its proteolytic degradation
Carter et al., Science 1996 : Selective activation of NF-kappa B by nerve growth factor through the neurotrophin receptor p75 ... Selective activation of NF-kappa B by nerve growth factor through the neurotrophin receptor p75 ... In the absence of TrkA, NGF binding to p75NGR activated the transcription factor nuclear factor kappa B (NF-kappa B) in rat Schwann cells ... The effect was selective for NGF ; NF-kappa B was not activated by BDNF or NT-3 ... The effect was selective for NGF ; NF-kappa B was not activated by BDNF or NT-3
Harhaj et al., Virology 1996 : In the present study, we demonstrate that Tat transactivation of the HIV-1 LTR is markedly inhibited by several cytoplasmic inhibitors of NF-kappa B/Rel , suggesting the critical involvement of these host transcription factors in the function of the viral Tat protein
Jimi et al., J Biol Chem 1996 : IL-1 also activated NF-kappaB induction in osteoblasts
Barger et al., Ann N Y Acad Sci 1996 : We report that NF-kappa B can also be induced by APPS
Cobb et al., Eur J Immunol 1996 : As expected, the protease inhibitors blocked the activation of nuclear factor (NF)-kappa B in response to IL-1 beta stimulation ... In contrast, norLEU did not prevent IL-1 beta induced nuclear translocation of NF-kappa B
Kleinert et al., J Biol Chem 1996 (Second Messenger Systems) : Transcription factor NF-kappaB is essential for the induction of nitric oxide synthase (NOS) II ( iNOS ) by bacterial lipopolysaccharide in murine macrophages ( Xie, Q. W., Kashiwabara, Y., and Nathan, C. ( 1994 ) J. Biol. Chem. 269, 4705-4708 ) ... Transcription factor NF-kappaB is essential for the induction of nitric oxide synthase (NOS) II ( iNOS ) by bacterial lipopolysaccharide in murine macrophages ( Xie, Q. W., Kashiwabara, Y., and Nathan, C. ( 1994 ) J. Biol. Chem. 269, 4705-4708 )
Kalkhoven et al., J Biol Chem 1996 (Neoplasms) : Furthermore, activation of NF-kappaB by tumor necrosis factor-alpha also results in repression of PR, while PR is able to repress tumor necrosis factor-alpha induced NF-kappaB activity
Welsh et al., J Biol Chem 1996 : Binding to the NF-kappaB element was strongly induced by IL-1beta , but not by acetylsphingosine or PMA
Shin et al., J Biol Chem 1996 : .NO donors had no inhibitory effect on IRF-1 activation but did inhibit basal and IL-1 alpha stimulated NF-kappa B activation ... These findings suggest that the induction of ICAM-1 and VCAM-1 expression requires NF-kappa B activation and that .NO attenuates IFN-gamma induced VCAM-1 expression primarily by inhibiting basal constitutive NF-kappa B activity in SMC ... These findings suggest that the induction of ICAM-1 and VCAM-1 expression requires NF-kappa B activation and that .NO attenuates IFN-gamma induced VCAM-1 expression primarily by inhibiting basal constitutive NF-kappa B activity in SMC
Good et al., J Virol 1996 : Persistent activation of NF-kappa B/Rel by human T-cell leukemia virus type 1 tax involves degradation of I kappa B beta
Jacqué et al., J Virol 1996 : The first was to abruptly terminate tumor necrosis factor induced NF-kappaB binding to the enhancer sequences in U1 monocytic cells, using a short pulse of exogenous tumor necrosis factor
Klement et al., Mol Cell Biol 1996 (Dermatitis) : IkappaBalpha deficiency results in a sustained NF-kappaB response and severe widespread dermatitis in mice ... Upon stimulation, rapid degradation of IkappaBalpha allows nuclear translocation of NF-kappaB
Gasparro et al., Photochem Photobiol 1996 : In cellular studies with UV or photoactivatable agents, the activation of NF-kappa B , probably the most widely studied transcription factor , occurs after its release from an inhibiting factor, I kappa B
Yamaoka et al., EMBO J 1996 (Cell Transformation, Neoplastic) : Stable expression of Tax competent for transformation caused enhanced DNA binding of NF-kappa B in rat fibroblasts ... We also demonstrate that stable co-expression of the NFKB2 precursor, known as a member of the I kappa B proteins, with wild-type Tax blocked transformation as well as eliminated aberrant NF-kappaB activation by Tax without interference with the HTLV-I LTR mediated trans-activation
Cohen et al., Proc Natl Acad Sci U S A 1996 : CNI-1493 blocked neither the lipopolysaccharide (LPS) induced increases in the expression of TNF mRNA nor the translocation of nuclear factor NF-kappa B to the nucleus in macrophages activated by 15 min of LPS stimulation, indicating that CNI-1493 does not interfere with early NF-kappa B-mediated transcriptional regulation of TNF ... CNI-1493 blocked neither the lipopolysaccharide (LPS) induced increases in the expression of TNF mRNA nor the translocation of nuclear factor NF-kappa B to the nucleus in macrophages activated by 15 min of LPS stimulation, indicating that CNI-1493 does not interfere with early NF-kappa B-mediated transcriptional regulation of TNF
de Wit et al., Exp Hematol 1996 : The effects of IFN-gamma on the transcription factors were specific, since no change was observed in the expression of NF-IL-6 or I kappa B alpha, the inhibitor of NF-kappa B ... The effects of IFN-gamma on the transcription factors were specific, since no change was observed in the expression of NF-IL-6 or I kappa B alpha , the inhibitor of NF-kappa B ... We conclude that the effects of IFN-gamma on the expression of the transcription factors AP-1 and NF-kappa B may be important for the modulatory effects of IFN-gamma on the cytokine expression in activated human monocytes
Sen et al., FEBS Lett 1996 : Involvement of intracellular Ca2+ in oxidant induced NF-kappa B activation ... In Jurkat cells that are not sensitive to H2O2 induced NF-kappaB activation, H2O2 potentiated NF-kappaB activation in the presence of sustained high [Ca2+ ] i following thapsigargin treatment
Perera et al., Infect Immun 1996 : The kinetics of NF-kappaB activation by paclitaxel are slower than those of LPS : by 15 min poststimulation, LPS induced NF-kappaB activation was readily detected, whereas the paclitaxel induced NF-kappaB activation was minimal ... Moreover, paclitaxel- and protein-free LPS induced translocation of NF-kappaB was seen only in macrophages derived from LPS-responsive C3H/OuJ mice and not from the LPS-hyporesponsive C3H/HeJ mice, a finding that is consistent with those of previous genetic studies linking paclitaxel responsiveness to the Lps gene
Folgueira et al., J Virol 1996 : Constitutively active p21ras ( v-H-Ras ) activated NF- kappa B-dependent transcription and induces the nuclear translocation of a bona fide p65/p50 heterodimer by targeting I kappa B alpha ... Because of the similarity between HIV and Ras-Raf induced NF-kappa B activation in monocytic cells, we next tested whether HIV induced NF-kappa B activation was mediated by the Ras-Raf signal transduction pathway
Lindholm et al., J Virol 1996 : Human T-cell lymphotropic virus type 1 Tax1 activation of NF-kappa B : involvement of the protein kinase C pathway ... Human T-cell lymphotropic virus type 1 Tax1 induces the activation and nuclear localization of the cellular transcription factor , NF-kappa B ... Human T-cell lymphotropic virus type 1 Tax1 induces the activation and nuclear localization of the cellular transcription factor, NF-kappa B
Muraoka et al., J Clin Invest 1996 : We further show that FK506 induces NF-kappaB regulated IL-6 production in vitro and in vivo, in particular in kidney
Rodriguez et al., Oncogene 1996 : Activation of transcription factor NF-kappaB involves signal induced degradation of the protein inhibitor IkappaB-alpha and release of NF-kappaB which translocates to the nucleus where it influences transcription of responsive genes
Kretz-Remy et al., J Cell Biol 1996 : Inhibition of I kappa B-alpha phosphorylation and degradation and subsequent NF-kappa B activation by glutathione peroxidase overexpression
Traenckner et al., J Cell Sci Suppl 1995 : In this paper, we report for the first time that higher molecular mass forms of I kappa B-alpha occur under conditions that lead to a phosphorylation of I kappa B-alpha and activation of NF-kappa B ... Our data suggest that conjugation of I kappa B-alpha with ubiquitin is an intermediate reaction in the phosphorylation controlled degradation of I kappa B-alpha and the subsequent activation of NF-kappa B
Good et al., Biochem Biophys Res Commun 1996 : Activation of the transcription factor NF-kappa B by various cellular stimuli involves phosphorylation and subsequent degradation of its inhibitor I kappa B alpha
Marinovich et al., Exp Cell Res 1996 : These findings suggest that TPT increases intracellular Ca2+ , alters actin polymerization and the cytoskeleton, and induces NF-kappaB activation, TNF-alpha synthesis, DNA degradation, and apoptosis
Modur et al., J Biol Chem 1996 : TNF additionally caused rapid p38 and JNK-1 mitogen activated protein kinase activation and efficient NF-kappaB translocation, which could not be achieved even by high levels of ceramide ... Thus activation of the ERK cascade alone is an incomplete endothelial cell stimulus, and the TNF receptor generates at least two signals : Raf-1 activation, which could be ceramide dependent ; and ceramide independent efficient NF-kappaB translocation and activation of p38 and JNK-1 mitogen activated kinases
Hirano et al., J Biol Chem 1996 : MEK kinase is involved in tumor necrosis factor alpha induced NF-kappaB activation and degradation of IkappaB-alpha ... These results suggest that MEK kinase is a signal mediator involved in TNFalpha induced NF-kappaB activation and that the activation of NF-kappaB by MEK kinase is regulated through the degradation of IkappaB-alpha
Freter et al., J Biol Chem 1996 : Platelet derived growth factor induction of the immediate-early gene MCP-1 is mediated by NF-kappaB and a 90-kDa phosphoprotein coactivator
Nakano et al., J Biol Chem 1996 (Lymphoma, B-Cell) : The transcription factor NF-kappaB is activated in HEK293 cells by overexpression of full-length TRAF5 but not a truncated form lacking the zinc binding region
Curtiss et al., Mol Cell Biol 1996 : This strongly suggests that activation of NF-kappaB is insufficient to activate transcription via the CD28RE-AP-1 element of the IL-2 promoter and that a different transcription factor , upregulated via the activation domain of the HTLV-1 Tax protein, may be involved
Franzoso et al., EMBO J 1996 : Activation of the serum response factor by p65/NF-kappaB
Good et al., EMBO J 1996 : In the present study, we demonstrate that neither NF-kappa B/Rel nor CREB/ATF is sufficient for Tax mediated activation of the IL-2 promoter
Umansky et al., Int Immunol 1996 (Newcastle Disease) : NDV mediated NO synthesis and NF-kappa B activation were blocked by an antioxidant ( butylated hydroxyanisole ), by an inhibitor of protein tyrosine kinase ( genistein ) and of protein kinase A ( H-89 ), but not by an inhibitor of protein kinase C ( staurosporin )
Guijarro et al., Nephrol Dial Transplant 1996 : Treatment of mesangial cells with lovstatin in the presence of exogenous cholesterol resulted in a significant reduction of the LPS induced NF-kappaB activity ... In the presence of either mevalonate or the mevalonate metabolite farnesyl pyrophosphate, the lovastatin inhibition of NF-kappaB activation was substantially reversed, supporting a role for mevalonate metabolites in LPS induced mesangial cell NF-kappaB activation
Sakurai et al., Biochim Biophys Acta 1996 (Glomerulonephritis...) : Pyrrolidine dithiocarbamate ( PDTC ), a potent inhibitor of NF-kappa B activation, inhibited the NTS induced increase of glomerular NF-kappa B DNA binding activity, followed by the inhibition of mRNA expression of IL-1 beta, MCP-1, ICAM-1 and iNOS, which are known to be regulated by NF-kappa B
Demarchi et al., J Virol 1996 : The effects of Tat on the enhancer are mediated by Tat induced nuclear translocation of NF-kappaB , which parallels the kinetics of transcriptional activation ... These results show that NF-kappaB activation by Tat is important for LTR transcriptional activation
Higuchi et al., J Immunol 1996 (Leukemia, Myeloid) : Since ceramide has been shown to play a role in TNF mediated activation of nuclear factor-kappa B (NF-kappa B) , we examined the effect of SR33557 on this early cellular response of TNF ... Surprisingly, this inhibitor of ceramide production was found to have no effect on TNF mediated NF-kappa B activation, thus suggesting that SR33557-sensitive acidic SMase is not involved in this process
Romano et al., J Immunol 1996 : IL-10 markedly reduces nuclear factor (NF)-kappa B/Rel nuclear activity induced in PBMC by stimulation with the anti-CD3 mAb OKT3 ... Our results demonstrate that IL-10 can inhibit the induction of NF-kappa B/Rel nuclear activity in CD3 stimulated T lymphocytes
Song et al., Proc Natl Acad Sci U S A 1996 : The tumor necrosis factor-inducible zinc finger protein A20 interacts with TRAF1/TRAF2 and inhibits NF-kappaB activation ... TRAF2 action is required for activation of the transcription factor NF-kappaB triggered by TNF and the CD40 ligand ... In cotransfection experiments, A20 blocked TRAF2 mediated NF-kappaB activation ... A20 also inhibited TNF and IL-1 induced NF-kappaB activation, suggesting that it may inhibit NF-kappaB activation signaled by diverse stimuli
Totpal et al., J Interferon Cytokine Res 1996 : Furthermore, TNF activated the nuclear transcriptional factor NF-kappa B in both cell types, whereas anti-Fas had no effect
Grimm et al., J Cell Biol 1996 : Bcl-2 inhibited NF-kappaB activity by an unusual mechanism
Mastronarde et al., J Infect Dis 1996 : Induction of interleukin (IL)-8 gene expression by respiratory syncytial virus involves activation of nuclear factor (NF)-kappa B and NF-IL-6
Dezube et al., J Cardiovasc Pharmacol 1995 (HIV Infections) : TNF also induces expression of human immunodeficiency virus ( HIV ) through activation of the transcription factor NF-kappa B , which binds to the viral long terminal repeat ( LTR )
Brostjan et al., J Biol Chem 1996 : Glucocorticoid mediated repression of NFkappaB activity in endothelial cells does not involve induction of IkappaBalpha synthesis ... More recently, glucocorticoid mediated induction of IkappaBalpha , an inhibitor of NFkappaB , has been described in monocytes and lymphocytes ; an increase in IkappaBalpha mRNA and protein resulted in inactivation and cytosolic retention of NFkappaB ... Although transcriptional activation by NFkappaB was significantly reduced in the presence of glucocorticoids, we did not detect induction of IkappaBalpha protein that could prevent nuclear translocation of NFkappaB upon stimulation with lipopolysaccharide or tumor necrosis factor alpha
Mori et al., Blood 1996 (Leukemia-Lymphoma, Adult T-Cell) : These data suggest a general role for NF-kappa B activation in the induction of IL-10 gene transcription
Dokter et al., Leukemia 1996 : Neither IL-10 nor IL-4 inhibited LPS induced NF-kappa B binding activity ... Neither IL-10 nor IL-4 inhibited LPS induced NF-kappa B binding activity ... Neither IL-10 nor IL-4 inhibited LPS induced NF-kappa B binding activity
Rothe et al., Proc Natl Acad Sci U S A 1996 : TRAF2 is required for CD40- and TNF mediated activation of the transcription factor NF-kappa B ... TRAF2 is required for CD40- and TNF mediated activation of the transcription factor NF-kappa B ... Overexpression of I-TRAF inhibits TRAF2 mediated NF-kappa B activation signaled by CD40 and both TNF receptors
Ramazzotti et al., AIDS 1996 : An enhanced nuclear factor (NF)-kappa B activation in response to tumour necrosis factor (TNF)-alpha has been observed in stably tat transfected cells ... Therefore, the possible association of a Tat autocrinous loop with the enhanced NF-kappa B-binding activity induced by TNF-alpha in Tat producing cells was studied by anti-Tat antibody blocking experiments ... Permanently tat transfected Jurkat cells, maintained either in the presence or absence of anti-Tat antibody, were studied for the presence of TNF-alpha induced NF-kappa B-binding activity ( quantified by electrophoretic mobility shift assays ) and the presence of cell-surface bound Tat ( determined by flow cytometry and confocal microscopy of anti-Tat immunofluorescence stained cell preparations ... The enhanced production of TNF-alpha induced NF-kappa B binding activity exhibited by tat transfected Jurkat cells was completely abolished in cell cultures maintained in the presence of anti-Tat antibody, thus indicating that the increased TNF-alpha induced NF-kappa B binding activity observed in Jurkat-tat cells was dependent on the presence of Tat protein in an antibody-accessible location
Navarro et al., AIDS 1996 : Moreover, PTX reduced TNF-alpha, IFN-gamma and IL-10 production and NF-kappa B activation
Drew et al., J Interferon Cytokine Res 1995 : We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence ( ICS ) ... We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence ( ICS ) ... We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence ( ICS )
Aragane et al., J Invest Dermatol 1996 : NF-IL-6 binding to a 32P labeled NF-IL-6 binding sequence was enhanced 20 min after TGF-alpha stimulation and returned to basal levels within 90 min, whereas NFkappaB binding activity was enhanced after 20 min and returned to normal 60 min after stimulation
Lin et al., Mol Cell Biol 1996 : Activation of NF-kappaB/Rel by CD40 engagement induces the mouse germ line immunoglobulin Cgamma1 promoter
Udagawa et al., J Immunol 1996 : Similar to expression of IL-2, we demonstrated that recombinant vascular-cell adhesion molecule-1 , when co-immobilized with anti-CD3 mAb, significantly enhanced the induction of transcription factors NF-AT, AP-1, and NF-kappa B as determined by electromobility shift assays ... Similar to expression of IL-2, we demonstrated that recombinant vascular-cell adhesion molecule-1 , when co-immobilized with anti-CD3 mAb, significantly enhanced the induction of transcription factors NF-AT, AP-1, and NF-kappa B as determined by electromobility shift assays
Arsura et al., Immunity 1996 : TGF beta 1 inhibits NF-kappa B/Rel activity inducing apoptosis of B cells : transcriptional activation of I kappa B alpha ... In WEHI 231 and CH33 cells, we observed an increase in I kappa B alpha , a specific NF-kappa B/Rel inhibitor , due to transcriptional induction
Warner et al., Am J Physiol 1996 : TNF-alpha increased nuclear factor (NF)-kappa B-DNA binding, an effect blocked by pretreatment with NAC
Bellezzo et al., Am J Physiol 1996 : Since the role of CD14 in LPS signaling has not been evaluated in Kupffer cells, the resident hepatic macrophage, the purpose of this study was to characterize LPS mediated NF-kappa B activation under CD14 dependent ( 1 % serum, as a source of LBP ) and CD14 independent ( serum-free ) conditions ... However, in Kupffer cells, NF-kappa B was activated by LPS under CD14 independent conditions, and this response was not potentiated by serum
Fox et al., Biochem Biophys Res Commun 1996 (Inflammation) : Such treatments lead to inhibition of LPS mediated NF-kappa B activation
Pahl et al., FEBS Lett 1996 : Activation of NF-kappa B by ER stress requires both Ca2+ and reactive oxygen intermediates as messengers ... Two distinct intracellular Ca2+ chelators and a panel of structurally unrelated antioxidants prevented NF-kappaB activation by various ER stress eliciting agents, whereas only antioxidants but not the Ca2+ chelators prevented NF-kappaB activation by the inflammatory cytokine TNF-alpha ... Consistent with an involvement of calcium, the ER-resident Ca2+-ATPase inhibitors thapsigargin and cyclopiazonic acid (CPA), which trigger a rapid efflux of Ca2+ from the ER, also potently activated NF-kappaB ... Consistent with an involvement of calcium, the ER-resident Ca2+-ATPase inhibitors thapsigargin and cyclopiazonic acid (CPA), which trigger a rapid efflux of Ca2+ from the ER, also potently activated NF-kappaB
Faggioli et al., Eur J Biochem 1996 (Breast Neoplasms...) : Nuclear factor kappa B (NF-kappa B) , nuclear factor interleukin-6 ( NFIL-6 or C/EBP beta ) and nuclear factor interleukin-6 beta ( NFIL6-beta or C/EBP delta ) are not sufficient to activate the endogenous interleukin-6 gene in the human breast carcinoma cell line MCF-7
Yao et al., Immunity 1995 : Recombinant HVS13 and CTLA8 stimulate transcriptional factor NF-kappa B activity and interleukin-6 (IL-6) secretion in fibroblasts, and costimulate T cell proliferation
Takeshita et al., J Immunol 1996 : Electrophoretic mobility shift assays showed that IL-4, but not IL-10 , inhibited nuclear NF-kappaB activity, and that IL-4 and IL-10 did not affect NF-IL-6 activity ... Electrophoretic mobility shift assays showed that IL-4 , but not IL-10, inhibited nuclear NF-kappaB activity, and that IL-4 and IL-10 did not affect NF-IL-6 activity ... These results suggest that IL-4 may inhibit the transcription of the IL-6 gene by affecting NF-kappaB binding activity, while IL-10 may inhibit the IL-6 mRNA levels post-transcriptionally, without suppressing promoter activity
O'Neill et al., Int J Clin Lab Res 1995 : Attention has also been focussed in the activation of the transcription factor NF kappa B , which is rapidly activated by IL1
Lee et al., Proc Natl Acad Sci U S A 1996 : We demonstrate here that TRAF2 also plays an important role in CD30 induced NF-kappa B activation ... We demonstrate here that TRAF2 also plays an important role in CD30 induced NF-kappa B activation ... We also show that TRAF2 mediated activation of NF-kappa B plays a role in the activation of HIV transcription induced by CD30 cross linking
Biswas et al., Mol Med 1994 : PTX inhibited PKC- or PKA catalyzed activation of NF-kappa B in cytoplasmic extracts from unstimulated Jurkat or 293-27-2 cells, but not interaction of preactivated NF-kappa B with its motifs ... The mechanism of inhibitory action of PTX on virus replication and NF-kappa B-induced transactivation of HIV-1 gene expression has been elucidated as due to blocking PKC dependent PMA- or TNF-alpha induced activation of NF-kappa B in Jurkat and 293-27-2 cells
Tewari et al., Curr Opin Genet Dev 1996 : Significantly, TRAF2 mediates the activation of NF-kappa B by both receptors ... Significantly, TRAF2 mediates the activation of NF-kappa B by both receptors ... TRADD , which contains a novel ` death domain ' that binds to the corresponding death domain in the cytoplasmic segment of TNFR1, can mediate both activation of NF-kappa B and induction of apoptosis, the two major responses signaled by TNFR1
Abril et al., Tissue Antigens 1996 (Adenocarcinoma...) : Comparison of kappa-B binding activity showed that IFN-alpha and IFN-gamma induced opposite changes in NF-kappa B binding activity in AGS cells, indicating that the absence of MHC class I response in AGS appears to be independent of kappa-B activity
Béraud et al., J Acquir Immune Defic Syndr Hum Retrovirol 1996 (Cell Transformation, Viral...) : The mechanisms by which Tax activates NF-kappa B remain unclear, and findings presented in the literature are often controversial
Imbert et al., Cell 1996 : Pervanadate induced I kappa B-alpha phosphorylation and NF-kappa B activation required expression of the T cell tyrosine kinase p56ick
Mackay et al., J Biol Chem 1996 : Tumor necrosis factor alpha (TNFalpha) and lymphotoxin alpha ( LTalpha, originally TNFbeta ) are potent nuclear factor kappaB (NFkappaB) activators in various cell types ... Tumor necrosis factor alpha (TNFalpha) and lymphotoxin alpha ( LTalpha, originally TNFbeta ) are potent nuclear factor kappaB (NFkappaB) activators in various cell types ... Here, we show that engagement of LTbeta-R with a soluble form of LTalpha1beta2 or with a specific anti-LTbeta-R agonistic monoclonal antibody CBE11 quickly induces activation of NFkappaB in HT-29 and WiDr human adenocarcinomas ... LTbeta-R triggering activates NFkappaB and induces proliferation in WI-38 human lung fibroblasts ... Thus, like several other members of the TNF receptor family, the LTbeta-R can activate NFkappaB following receptor ligation in some but not all LTbeta-R positive cells
Guerrini et al., FEBS Lett 1996 : NF-kappa B-mediated regulation of urokinase gene expression by PMA and TNF-alpha in human A549 cells ... Using the protein synthesis inhibitor cycloheximide and the antioxidant PDTC, that have an opposite effect on NF-kappa B and AP-1 activation, we showed that uPA mRNA induction by TNF-alpha and PMA in the A549 cell line is mainly due to NF-kappa B activation
Chainy et al., J Immunol 1996 (Leukemia, Erythroblastic, Acute...) : Overall, our results clearly demonstrate that the activation of NF-kappa B and cytotoxicity by TNF is differentially regulated through the p60 receptor
Knecht et al., Oncogene 1996 (Carcinoma...) : Therefore, LMP1 mediated NF-kappa B activation appears to be involved in polycaria formation
Tewari et al., Arch Oral Biol 1996 (Periodontitis) : During earlier examination of interleukin-1 (IL-1) induced matrix metalloproteinase gene expression in human gingival fibroblasts a highly induced immediate early gene, I kappa B-alpha , a NF kappa B DNA binding inhibitor , was identified ... The aim now was to investigate whether recombinant ( r ) IL-1 beta induces the stimulation of NF kappa B and its inhibitor proteins in human gingival fibroblasts and to understand if inhibition of its activity affects collagenase gene expression
Tong et al., J Neurosci Res 1996 : Effect of nerve growth factor on AP-1, NF-kappa B , and Oct DNA binding activity in apoptotic PC12 cells : extrinsic and intrinsic elements
Suyang et al., Mol Cell Biol 1996 : Role of unphosphorylated, newly synthesized I kappa B beta in persistent activation of NF-kappa B
Kitajima et al., J Bone Miner Res 1996 : HTLV-I tax protein and TNF-alpha induced activation of NF-kappa B in apoptotic MC3T3-E1 cells
Keller et al., J Biol Chem 1996 (Prostatic Neoplasms) : It did not appear that 5alpha-dihydrotestosterone inhibited NFkappaB by activating the androgen receptor to compete for the NFkappaB response element as we could not detect androgen receptor binding to the IL-6 promoter by DNase I footprinting assay ... Finally, we confirmed that IkappaBalpha inhibits NFkappaB mediated activation of the interleukin-6 promoter
Ware et al., J Cell Biochem 1996 : The TRAF family of Zn RING/finger proteins bind to TNFR80 ; CD40 and LT beta R are involved in induction NF kappa B and cell survival ... The TRAF family of Zn RING/finger proteins bind to TNFR80 ; CD40 and LT beta R are involved in induction NF kappa B and cell survival
Beck et al., FEBS Lett 1996 : Cloning and sequencing of the proximal promoter of the rat iNOS gene : activation of NFkappaB is not sufficient for transcription of the iNOS gene in rat mesangial cells ... To further investigate the molecular mechanisms and the role of NFkappaB in cytokine elicited iNOS transcription, we cloned a 661 bp genomic rat DNA fragment, which contains 497 bp of the proximal iNOS promoter ... We found that binding of NFkappaB is strongly induced in mesangial cells by both IL-1beta and TNF-alpha ... We found that binding of NFkappaB is strongly induced in mesangial cells by both IL-1beta and TNF-alpha
Ginn-Pease et al., Biochem Biophys Res Commun 1996 : However, signals induced by oxidative conditions effectively cooperated with stimulatory signals provided by PMA but not with T cell receptor/CD3 stimulation to induce significant increases in NF kappa B CAT responses
Sato et al., J Rheumatol 1996 (Arthritis, Rheumatoid) : Our data suggest that TNF-alpha induces expression of proinflammatory cytokines such as IL-8 and MCP-1 through generation of reactive oxygen intermediates and subsequent activation of NF-kappa B in human synovial cells, and the antioxidants may inhibit, at least in part, the activation of NF-kappa B by TNF-alpha ... Our data suggest that TNF-alpha induces expression of proinflammatory cytokines such as IL-8 and MCP-1 through generation of reactive oxygen intermediates and subsequent activation of NF-kappa B in human synovial cells, and the antioxidants may inhibit, at least in part, the activation of NF-kappa B by TNF-alpha ... Our data suggest that TNF-alpha induces expression of proinflammatory cytokines such as IL-8 and MCP-1 through generation of reactive oxygen intermediates and subsequent activation of NF-kappa B in human synovial cells, and the antioxidants may inhibit, at least in part, the activation of NF-kappa B by TNF-alpha
Cao et al., Nature 1996 : When overexpressed in human 293 cells, TRAF6 activates NF-kappaB
Hess et al., Neurosci Lett 1996 : TNF activated nuclear factor kappa B (NF kappa B) in HBMEC
McGillis et al., Can J Physiol Pharmacol 1995 : To determine whether the inhibitory effects of CGRP on sIg expression are mediated by an inhibition of NF kappa-B translocation to the nucleus, electrophoretic mobility shift assays were performed using nuclear proteins from 70Z/3 cells ... There was no difference in NF kappa-B binding activity in cells that had been treated with LPS or LPS + CGRP, suggesting that the inhibitory effect of CGRP is not mediated by an inhibition of NF kappa-B activity
Fujisawa et al., Arthritis Rheum 1996 : To examine whether nuclear factor kappaB (NF-kappaB) is activated in cultured synovial cells in response to tumor necrosis factor alpha (TNFalpha) and to investigate the correlation between NF-kappaB activation and synovial cell proliferation ... Stimulation of synovial cells with TNFalpha activated NF-kappaB and subsequent transcription of several genes ... Treatment of synovial cells with N-acetyl-L-cysteine (NAC), an antioxidant agent, inhibited TNFalpha induced NF-kappaB activation and transcription ... Our results suggest that NF-kappaB plays a pivotal role in synovial cell activation by TNFalpha
Makropoulos et al., Arch Toxicol 1996 : Analysis of DNA binding revealed that NF-kappa B activation in response to TNF was significantly inhibited under these conditions
Schafer et al., Virology 1996 : Thus it appears that p65 plays a limited role in the NF-kappa B-dependent activation of the HIV-1 LTR following HSV-1 infection and that the stimulation is mediated by the p50/p65 and p65/c-rel heterodimers
Lieb et al., J Neurochem 1996 (Astrocytoma) : Here, we describe that both IL-1 beta and TNF alpha activate the transcription factor nuclear factor-kappa B (NF-kappa B) via production of reactive oxygen intermediates resulting in ACT expression ... Here, we describe that both IL-1 beta and TNF alpha activate the transcription factor nuclear factor-kappa B (NF-kappa B) via production of reactive oxygen intermediates resulting in ACT expression
Wang et al., Science 1996 (Neoplasms) : The activation of the transcription factor nuclear factor-kappa B (NF-kappaB) by tumor necrosis factor (TNF) , ionizing radiation, or daunorubicin ( a cancer chemotherapeutic compound ), was found to protect from cell killing
Hecker et al., Br J Pharmacol 1996 : The inhibitory effect of DCI on NF-kappa B activation, however, does not seem to be related to its antioxidative properties, since DCI, unlike chrysin or NAS, is a potent serine protease inhibitor which stabilizes the inactive NF-kappa B complex by protecting the inhibitory I kappa B-alpha subunit from proteolytic degradation
Munroe et al., J Inflamm 1995 : Overexpression of PGHS1 strongly potentiated NF-kappa B activation by phorbol esters and dramatically elevated the generation of intracellular reactive oxygen species ( ROS ) in response to low concentrations of t-butyl peroxide
Shatrov et al., AIDS Res Hum Retroviruses 1996 : HIV type 1 glycoprotein 120 amplifies tumor necrosis factor induced NF-kappa B activation in Jurkat cells ... Taken together, our data suggest that gp120 potentiates TNF induced NF-kappa B activation by stimulating a signal pathway that involves p56lck and the increased formation of reactive oxygen intermediates such as H2O2
Edmead et al., J Immunol 1996 : Using selective inhibitors, we investigated the signaling pathways involved in the CD28 mediated induction of AP-1 and NF-kappaB
Maziere et al., Biochem Mol Biol Int 1996 : The effect of cupric ions or endothelial cell oxidized LDL on the transcription factor NF kappa B activation was investigated by electrophoretic mobility shift assay ... Oxidized LDL induced NF kappa B activation in fibroblasts, endothelial and smooth muscle cells
Goodman et al., Transplantation 1996 : We investigated whether overexpression of I(kappa)B(alpha) in EC, using adenoviral gene transfer, could block NF-kappaB translocation, thereby inhibiting NK cell mediated EC activation
Ouyang et al., Toxicol Appl Pharmacol 1996 (Thymoma) : Using Western blot analysis, effects on EL-4 cells were further related to VT-mediated inhibition of resynthesis of IkappaBalpha , a cytoplasmic inhibitor of NF-kappaB/Rel
Van Antwerp et al., Mol Cell Biol 1996 : Therefore, we conclude that both association with NF-(kappa)B and a PEST sequence are not required for signal induced I(kappa)B(alpha) degradation
Franken et al., J Virol 1996 : Nevertheless, the simian EBV LMP1s retain most functions in common with EBV LMP1, including the ability to induce NF-(kappa)B activity in human cells, to bind the tumor necrosis factor associated factor 3 ( TRAF3 ) in vitro, and to induce expression of tumor necrosis factor-responsive genes, such as ICAM1 , in human B lymphocytes
de Vera et al., Hepatology 1996 : The addition of the nuclear factor kappaB (NF-kappaB) inhibitor pyrrolidine dithiocarbamate also inhibited NOS2 expression, suggesting a role for NF-kappaB in the cytokine induction of NOS2 in hepatocytes
Singh et al., J Immunol 1996 (Leukemia, Myelomonocytic, Acute) : Viral replication, immune regulation, and induction of various inflammatory and growth-regulatory genes require activation of a nuclear transcription factor ( NF)-kappa B ... The pretreatment of human myeloid ML-1a cells with capsaicin blocked TNF mediated activation of NF-kappa B in a dose- and time dependent manner
Hölschermann et al., Blood 1996 (Coronary Artery Disease...) : Electrophoretic mobility shift assay showed that CsA inhibited the LPS induced activation of the nuclear factor kappa B (NF-kappa B)
Simon et al., Int Immunol 1996 : Second, antigen induced a different pattern of transcription factor binding activities than PMA/lonomycin in DP thymocytes, AP-1 activity being selectively induced by antigen and NF-kappa B by PMA/lonomycin
Sakurada et al., Int Immunol 1996 (Arthritis, Rheumatoid) : The role of transcription factor NF-kappa B in the induction of cytokines and ICAM-1 upon stimulation with proinflammatory cytokines, IL-1 and tumor necrosis factor (TNF)-alpha was investigated in primary synovial fibroblasts obtained from patients with rheumatoid arthritis ( RA )
Farivar et al., J Biol Chem 1996 : Tumor necrosis factor-alpha and interferon-gamma induced NF-kappaB ( p50/p65 ) and STAT-1, respectively, as assessed by gel shift assays ... Tumor necrosis factor-alpha and interferon-gamma induced NF-kappaB ( p50/p65 ) and STAT-1, respectively, as assessed by gel shift assays
Shu et al., Proc Natl Acad Sci U S A 1996 : The association of TRADD, a 34-kDa cytoplasmic protein containing a C-terminal death domain, with aggregated TNF receptor 1 (TNF-R1) through their respective death domains leads to NF-kappa B activation and programmed cell death ... The association of TRADD , a 34-kDa cytoplasmic protein containing a C-terminal death domain, with aggregated TNF receptor 1 (TNF-R1) through their respective death domains leads to NF-kappa B activation and programmed cell death
Harhaj et al., Mol Cell Biol 1996 : In this report, we show that CD28 costimulation leads to biphasic induction of NF-kappaB/Rel heterodimers, including early-phase induction of p50/RelA and c-Rel/RelA and late-phase induction of p50/c-Rel ... Interestingly, activation of these NF-kappaB/Rel complexes by the CD28 signal is associated with the rapid degradation of both IkappaBalpha and IkappaBbeta, two major cytoplasmic inhibitors of NF-kappaB/Rel ... Interestingly, activation of these NF-kappaB/Rel complexes by the CD28 signal is associated with the rapid degradation of both IkappaBalpha and IkappaBbeta , two major cytoplasmic inhibitors of NF-kappaB/Rel ... Interestingly, activation of these NF-kappaB/Rel complexes by the CD28 signal is associated with the rapid degradation of both IkappaBalpha and IkappaBbeta, two major cytoplasmic inhibitors of NF-kappaB/Rel
Devergne et al., Mol Cell Biol 1996 (Cell Transformation, Viral) : NF-kappaB activation by LMP1 ( 1-231 ) is likely to be mediated by TRAF1/TRAF2 heteroaggregates since TRAF1 is unique among the TRAFs in coactivating NF-kappaB with LMP1 ( 1-231 ), a TRAF2 dominant negative mutant can block LMP1 ( 1-231 ) -mediated NF-kappaB activation as well as TRAF1 coactivation, and 30 % of TRAF2 is associated with TRAF1 in EBV transformed B cells ... NF-kappaB activation by LMP1 ( 1-231 ) is likely to be mediated by TRAF1/TRAF2 heteroaggregates since TRAF1 is unique among the TRAFs in coactivating NF-kappaB with LMP1 ( 1-231 ), a TRAF2 dominant negative mutant can block LMP1 ( 1-231 ) -mediated NF-kappaB activation as well as TRAF1 coactivation, and 30 % of TRAF2 is associated with TRAF1 in EBV transformed B cells
Sulciner et al., Mol Cell Biol 1996 : In addition, expression of a dominant negative rac1 mutant ( N17rac1 ) inhibits basal and interleukin 1beta stimulated NF-kappaB activity
Ting et al., EMBO J 1996 : RIP mediates tumor necrosis factor receptor 1 activation of NF-kappaB but not Fas/APO-1 initiated apoptosis ... We have created mutant cell lines that are unable to activate NF-kappaB in response to TNF
Osnes et al., Thromb Haemost 1996 : Acetylsalicylic acid and sodium salicylate inhibit LPS induced NF-kappa B/c-Rel nuclear translocation, and synthesis of tissue factor ( TF ) and tumor necrosis factor alfa ( TNF-alpha ) in human monocytes
Kannabiran et al., Mol Cell Biol 1997 : The target site is contained within the interleukin response element ( ILRE ), which mediates IL-6 activation by NF-kappa B
Jobin et al., J Immunol 1997 : IL-1beta stimulated NF-kappaB nuclear translocation and NF-kappaB dependent IL-1beta and IL-8 expression in both Caco-2 and HT-29 cells as assayed by electrophoretic mobility shift assay, immunofluorescence, kappaB-luciferase transfection, reverse transcriptase-PCR analysis and ELISA ... IL-1beta stimulated NF-kappaB nuclear translocation and NF-kappaB dependent IL-1beta and IL-8 expression in both Caco-2 and HT-29 cells as assayed by electrophoretic mobility shift assay, immunofluorescence, kappaB-luciferase transfection, reverse transcriptase-PCR analysis and ELISA ... These data show that IL-1beta induces NF-kappaB activity and expression of NF-kappaB dependent genes in colonic epithelial cells and suggest altered regulation of IkappaB alpha degradation compared with other cell lineages, which may result in their increased responsiveness to therapeutic blockade
Yanagihara et al., J Allergy Clin Immunol 1996 : n-Acetyl-L-cysteine (NAC) , a potent antioxidant, blocked NF-kappa B activation caused by IL-4 and by anti-CD40 mAb ... These results indicate that NF-kappa B activity is commonly inducible in DND39 cells by IL-4 and anti-CD40 mAb and suggest that NF-kappa B sensitive to NAC may play a role in regulating IgE synthesis in B cells
Aizawa et al., Gene 1996 : Transient overexpression of rat CD30 in TARL-2 cells activated HIV LTR in a NF-kappa B site dependent manner, indicating that CD30 signals activate NF-kappa B
Miller et al., J Virol 1997 : The Epstein-Barr virus ( EBV ) -encoded LMP1 protein induces several cellular changes including induction of epidermal growth factor receptor (EGFR) expression and activation of the NF-kappaB transcription factor
de-Mello et al., Biochem Pharmacol 1996 (Insulinoma...) : Moreover, ebselen did not prevent IL-1 beta induced NF-kappa B activation
Klein-Hessling et al., Proc Natl Acad Sci U S A 1996 (Leukemia, T-Cell...) : It has been shown that HMG I(Y) proteins enhance NF-kappa B binding to the interferon beta promoter and virus mediated interferon beta promoter induction
Marsters et al., Curr Biol 1996 : Ectopic expression of Apo-3 also induced the activation of NF-kappa B
Cai et al., J Biol Chem 1997 (Breast Neoplasms) : Our results demonstrated that the mutated IkappaB alpha was stably expressed in the transfected MCF7 cells and blocked the TNF induced NF-kappaB nuclear translocation ... The nuclear NF-kappaB released from the endogenous IkappaB alpha within 30 min of TNF treatment was rapidly inhibited by the mutated IkappaB alpha
Fiedler et al., Am J Physiol 1996 (Respiratory Syncytial Virus Infections) : Mutational analysis of the 200-bp 5'-untranslated region of the IL-8 gene demonstrated that activation of NF-kappa B and NF-IL-6 were required for RSV induced transcriptional activation of the IL-8 gene
Aizawa et al., J Biol Chem 1997 : Tumor necrosis factor receptor associated factor ( TRAF) 5 and TRAF2 are involved in CD30 mediated NFkappaB activation ... Tumor necrosis factor receptor associated factor ( TRAF) 5 and TRAF2 are involved in CD30 mediated NFkappaB activation ... Overexpression of the TRAF domain of TRAF2 or TRAF5 showed a dominant negative effect on CD30 mediated NFkappaB activation ... Taken together, our results indicate that TRAF2 and TRAF5 directly interact with CD30 and are involved in NFkappaB activation by CD30 signaling
Conte et al., FEBS Lett 1997 : In this study we have investigated the effect of FCM on the LPS induced DNA binding activity of NF-kappaB , by means of electrophoretic shift assay ( EMSA ) ... We provide evidence that FCM strongly inhibits the LPS induced NF-kappaB activation in PBM ... On the other hand, FCM produced in the presence of indomethacin does not inhibit NF-kappaB activation by LPS
Suzuki et al., Nihon Geka Gakkai Zasshi 1996 : DSG may bind to Hsc 70 and inhibit the translocation of NF-kappa B into nucleus
Miller et al., Exp Cell Res 1997 : In a stable mouse L cell clone, L-15, NF-kappaB DNA binding activity induced by uv-C ( 254 nm ) but not by tumor necrosis factor-alpha (TNF-alpha) or 12-O-tetra-decanoylphorbol-13-acetate ( TPA ) correlated with the stimulation of HIV-LTR directed chloramphenicol acetyltransferase (CAT) activity ; uv-C was more effective than uv-B ( 312 nm ), while uv-A ( 365 nm ) had little effect on CAT activity
Sakitani et al., Hepatology 1997 : Sodium salicylate markedly inhibited the NO formation induced by IL-1beta, but did not affect NF-kappaB activation and iNOS mRNA induction
Ray et al., DNA Cell Biol 1997 (Inflammation) : LPS , on the other hand, activated NF-kappaB and C/EBP, which were detected only at the early phase of induction process
Bonizzi et al., Eur J Biochem 1996 : In this report, we first demonstrated that IL-1 beta is a potent activator of NF-kappa B in various epithelial transformed cell lines ( OVCAR-3, SKOV-3, MCF7 A/Z ) ... In these cells, IL-1 beta rapidly induces NF-kappa B through a complete degradation of I kappa B-alpha, while H2O2 activates NF-kappa B with slower kinetics through a partial degradation of I kappa B-alpha, p100 and p105 ... In these cells, IL-1 beta rapidly induces NF-kappa B through a complete degradation of I kappa B-alpha, while H2O2 activates NF-kappa B with slower kinetics through a partial degradation of I kappa B-alpha, p100 and p105 ... In these cells, IL-1 beta rapidly induces NF-kappa B through a complete degradation of I kappa B-alpha , while H2O2 activates NF-kappa B with slower kinetics through a partial degradation of I kappa B-alpha, p100 and p105 ... We showed that IL-1 beta mediated induction of NF-kappa B in OVCAR-3 and in other epithelial cell lines does not proceed through the production of reactive oxygen intermediates, while the same cytokine activates NF-kappa B in lymphoid cells through the intracellular generation of H2O2
Paal et al., Nucleic Acids Res 1997 : The co-transfection of TBP and E1A 13S stimulated NF-kappaB p65 dependent gene expression, showing the biological significance of these interactions
Lee et al., Immunol Lett 1996 : The NF-kappa B inhibitor, tepoxalin, suppresses surface expression of the cell adhesion molecules CD62E , CD11b/CD18 and CD106 ... The NF-kappa B inhibitor, tepoxalin, suppresses surface expression of the cell adhesion molecules CD62E, CD11b/CD18 and CD106 ... The NF-kappa B inhibitor, tepoxalin, suppresses surface expression of the cell adhesion molecules CD62E, CD11b/CD18 and CD106 ... The NF-kappa B inhibitor, tepoxalin, suppresses surface expression of the cell adhesion molecules CD62E, CD11b/CD18 and CD106
Parra et al., Mol Cell Biol 1997 : Both LFA-3 costimulation and B7-1 costimulation induced the AP-1 and NF-kappaB nuclear factors
Duckett et al., Mol Cell Biol 1997 : NF-kappaB induction through domain 2 was inhibited by coexpression of either full-length TRAF3 or dominant negative forms of TRAF1 or TRAF2 ... Together, these data support a model in which CD30 can induce NF-kappaB by both TRAF dependent and -independent mechanisms ... TRAF dependent induction of NF-kappaB appears to be regulated by the relative levels of individual TRAF proteins in the cell
Weiss et al., J Immunol 1997 : TNFR80 induces no cytotoxicity on its own, but is functional in these transfectants, as selective stimulation activates nuclear factor-kappaB (NF-kappaB) and induces NF-kappaB dependent cellular responses ( IL-6 and manganese superoxide dismutase ) ... TNFR80 induces no cytotoxicity on its own, but is functional in these transfectants, as selective stimulation activates nuclear factor-kappaB (NF-kappaB) and induces NF-kappaB dependent cellular responses ( IL-6 and manganese superoxide dismutase )
Tsitsikov et al., Proc Natl Acad Sci U S A 1997 : Transient overexpression of TRAF2 , but not TRAF1, induced NF-kappaB activation and HIV-1-long terminal repeat-driven transcription in the T cell line, KT3 ... Transient overexpression of TRAF2, but not TRAF1 , induced NF-kappaB activation and HIV-1-long terminal repeat-driven transcription in the T cell line, KT3 ... Moreover, dominant negative mutants consisting of the TRAF domain of TRAF1 and TRAF2 inhibited CD30 induction of NF-kappaB activation and HIV-1 transcription ... These results suggest that CD30 ligation may enhance the expression of HIV via TRAF-2 mediated activation of NF-kappaB
Renard et al., Biochem Pharmacol 1997 : In this study, we systematically evaluated the influence of glutathione peroxidase, glutathione reductase and catalase on IL-1 induced NF-kappa B activation by analysing the effect of specific inhibitors of these enzymes
Antras-Ferry et al., FEBS Lett 1997 : Moreover this glucocorticoid did not suppress the TNF-alpha induced increase of NF-kappaB binding activity
Jin et al., Cell 1997 : Transfection of macrophages with SLPI suppressed LPS induced activation of NF-kappa B and production of nitric oxide and TNF alpha
Muraoka et al., Transplantation 1997 (Anoxia...) : Hypoxia, but not reoxygenation, induces interleukin 6 gene expression through NF-kappa B activation ... We have previously shown that NF-kappa B plays an essential and indispensable role in the transcriptional activation of the IL-6 gene induced by various stimuli, including IL-1 and tumor necrosis factor-alpha ... This hypoxia induced NF-kappa B activation resulted in the kappa B-dependent transcriptional activation of the IL-6 gene
Crofford et al., Arthritis Rheum 1997 (Arthritis, Rheumatoid) : To evaluate involvement of the transcription factor nuclear factor kappa B (NF-kappa B) in the increased expression of cyclooxygenase-2 (COX-2) stimulated by interleukin-1 beta (IL-1 beta) in primary rheumatoid synoviocytes ... IL-1 beta rapidly stimulated the translocation of the p65, p50, and c-rel NF-kappa B subunits from the cytoplasm to the nucleus
Pindolia et al., Hematopathol Mol Hematol 1996 : Whereas AP-1 and SP-1 are constitutively expressed in stromal cells, NF-kB is detected only after stimulation with IL-1 ... Whereas AP-1 and SP-1 are constitutively expressed in stromal cells, NF-kB is detected only after stimulation with IL-1 ... Furthermore, while unable to induce the activation of NF-kB alone, IL-4 enhanced the activation of NF-kB by IL-1 ... Furthermore, while unable to induce the activation of NF-kB alone, IL-4 enhanced the activation of NF-kB by IL-1
Perona et al., Genes Dev 1997 : The human RhoA, CDC42 , and Rac-1 proteins efficiently induce the transcriptional activity of nuclear factor kappaB (NF-kappaB) by a mechanism that involves phosphorylation of Ikappa Balpha and translocation of p50/p50 and p50/p65 dimers to the nucleus, but independent of the Ras GTPase and the Raf-1 kinase ... The human RhoA , CDC42, and Rac-1 proteins efficiently induce the transcriptional activity of nuclear factor kappaB (NF-kappaB) by a mechanism that involves phosphorylation of Ikappa Balpha and translocation of p50/p50 and p50/p65 dimers to the nucleus, but independent of the Ras GTPase and the Raf-1 kinase ... The human RhoA, CDC42, and Rac-1 proteins efficiently induce the transcriptional activity of nuclear factor kappaB (NF-kappaB) by a mechanism that involves phosphorylation of Ikappa Balpha and translocation of p50/p50 and p50/p65 dimers to the nucleus, but independent of the Ras GTPase and the Raf-1 kinase ... We also show that activation of NF-kappaB by TNFalpha depends on CDC42 and RhoA, but not Rac-1 proteins, because this activity is drastically inhibited by their respective dominant negative mutants ... In contrast, activation of NF-kappaB by UV light was not affected by Rho , CDC42, or Rac-1 dominant negative mutants ... In contrast, activation of NF-kappaB by UV light was not affected by Rho, CDC42 , or Rac-1 dominant negative mutants ... In contrast, activation of NF-kappaB by UV light was not affected by Rho, CDC42, or Rac-1 dominant negative mutants
Pahl et al., Trends Biochem Sci 1997 : Both the release of Ca2+ from the ER and the subsequent production of reactive oxygen intermediates are required for ER-overload mediated NF-kappa B activation
Cogswell et al., J Exp Med 1997 : Involvement of Egr-1/RelA synergy in distinguishing T cell activation from tumor necrosis factor-alpha induced NF-kappa B1 transcription ... Characterization of the NF-kappa B1 promoter identified an Egr-1 site which was found to be essential for both the PMA/PHA mediated induction as well as the synergistic activation observed after the expression of the RelA subunit of NF-kappa B and Egr-1 ... Furthermore, Egr-1 induction was required for endogenous NF-kappa B1 gene expression, since PMA/PHA stimulated T cell lines expressing antisense Egr-1 RNA were inhibited in their ability to upregulate NF-kappa B1 transcription
Arenzana-Seisdedos et al., J Cell Sci 1997 : I kappa B alpha tightly regulates the transcriptional activity of NF-kappa B by retaining it in the cytoplasm in an inactive form
Bauer et al., Eur J Biochem 1997 : Moreover, known inhibitors of NF-kappa B activation, such as dexamethasone and the antioxidant pyrrolidine dithiocarbamate, as well as the protein kinase C ( PKC ) inhibitor Gö6976, strongly reduced lipopolysaccharide induced COX-2 transcription, indicating the involvement of NF-kappa B and PKC in COX-2 expression
Durandy et al., J Immunol 1997 (Hypergammaglobulinemia...) : CD40 triggered activation events, i.e., phosphatidylinositol 3 (PI3) kinase activation and induction of transcription factors NF-kappaB and AP-1, were next analyzed in B cell lines derived from five patients ... Three distinct patterns were observed : an absence of detectable abnormalities ( n = 1 ), defective PI3 kinase activation with normal induction of NF-kappaB and AP-1 ( n = 3 ), and defects in both PI3 kinase activation and induction of NF-kappaB and AP-1 (n = 1)
Dimitrov et al., J Virol 1997 : Under the same conditions, Ad infection did not inhibit TNF induced phosphorylation of cPLA2 or TNF activation of NFkappaB ... Under the same conditions, Ad infection did not inhibit TNF induced phosphorylation of cPLA2 or TNF activation of NFkappaB
Kurose et al., J Clin Invest 1997 : CD18/ICAM-1 dependent oxidative NF-kappaB activation leading to nitric oxide production in rat Kupffer cells cocultured with syngeneic hepatoma cells ... CD18/ICAM-1 dependent oxidative NF-kappaB activation leading to nitric oxide production in rat Kupffer cells cocultured with syngeneic hepatoma cells
Browning et al., J Biol Chem 1997 : The activation of NF-kappaB by fMLF appeared to be cell-specific and different from the activation of NF-kappaB by tumor necrosis factor-alpha (TNFalpha)
Legrand-Poels et al., Biochem Pharmacol 1997 : In this paper, we demonstrate that the activation of NF-kappaB by LPS in a promonocytic cell line ( U937 ) followed a rather slow kinetics, depending on the rate of IkappaB-alpha inhibitor hydrolysis ... However, the NF-kappaB activation pathway involving the acidic sphingomyelinase of the endolysosomial membrane did not seem to participate in the LPS induced NF-kappaB activation in U937 cells
Park et al., Biochem J 1997 : Nitric oxide regulates nitric oxide synthase-2 gene expression by inhibiting NF-kappaB binding to DNA
Chen et al., Biochem Biophys Res Commun 1997 : The results indicate that tetrandrine can inhibit the activation of NF-kappa B and NF-kappa B-dependent reporter gene expression by LPS , PMA, and silica in a dose dependent manner
Wong et al., Biochem Biophys Res Commun 1997 : The heat shock response inhibits inducible nitric oxide synthase gene expression by blocking I kappa-B degradation and NF-kappa B nuclear translocation
Yanagihara et al., Cell Immunol 1997 (Burkitt Lymphoma) : In addition, N-acetyl-L-cysteine (NAC) , a potent antioxidant, decreased NF-kappa B activation caused by IL-4, anti-CD40 mAb, or their combination ... These results indicate that IL-4 and ligation of CD40 induce NF-kappa B expression via at least a mechanism dependent on the PI3-kinase pathway and suggest that NF-kappa B sensitive to NAC may play a role in regulating germline C epsilon transcription
Basile et al., J Biol Chem 1997 : Role of NF-kappaB in tumor necrosis factor-alpha and interleukin-1beta regulation ... Role of NF-kappaB in tumor necrosis factor-alpha and interleukin-1beta regulation
Simoni et al., Artif Cells Blood Substit Immobil Biotechnol 1997 : We found that GLUT-Hb rapidly activated NF-kappa B and induced nuclear translocation
Zhu et al., Biochim Biophys Acta 1997 : The increases of ICAM-1 mRNA and NF-kappa B binding were inhibited by the protein tyrosine kinase inhibitors, genistein and lavendustin A, but not by inhibitors for cyclic AMP dependent protein kinases or protein kinase C
McGilvray et al., J Biol Chem 1997 : Inhibition of tyrosine kinase activity with genistein ( 10 microg/ml ) as well as selective MAP kinase inhibition with the MEK-1 inhibitor PD98059 abolished the VLA-4 dependent ERK tyrosine phosphorylation, inhibited NF kappaB nuclear binding, and abrogated tissue factor expression induced by both VLA-4 cross linking and adhesion to fibronectin in THP-1 cells and human peripheral blood monocytes
Lee et al., J Exp Med 1997 : TRAF interacting protein (TRIP) : a novel component of the tumor necrosis factor receptor (TNFR)- and CD30-TRAF signaling complexes that inhibits TRAF2 mediated NF-kappaB activation ... When associated, TRIP inhibits the TRAF2 mediated NF-kappaB activation that is required for cell activation and also for protection against apoptosis ... When associated, TRIP inhibits the TRAF2 mediated NF-kappaB activation that is required for cell activation and also for protection against apoptosis
Weih et al., J Exp Med 1997 (Abnormalities, Multiple...) : These results indicate that the lack of RelB is, in part, compensated by other p50 containing complexes and that the `` classical '' p50-RelA-NF-kappaB activity is not required for the development of the inflammatory phenotype
Unlap et al., Brain Res Mol Brain Res 1997 : Dexamethasone ( 2 mg/kg ) administration decreased NF-kappa B DNA binding in cerebral cortex and hippocampus nuclear extracts, maximally at 3-6 h after dexamethasone, followed by recovery at 24 h. Dexamethasone did not inhibit NF-kappa B by increasing the level of the inhibitory protein I kappa B alpha , as occurs in some peripheral cells, but instead lowered I kappa B alpha levels
Tachado et al., Proc Natl Acad Sci U S A 1997 : Both signals collaborate in regulating the downstream NF-kappa B/rel dependent gene expression of interleukin 1alpha, tumor necrosis factor (TNF) alpha, and inducible NO synthase ... Both signals collaborate in regulating the downstream NF-kappa B/rel dependent gene expression of interleukin 1alpha , tumor necrosis factor (TNF) alpha, and inducible NO synthase ... Both signals collaborate in regulating the downstream NF-kappa B/rel dependent gene expression of interleukin 1alpha, tumor necrosis factor (TNF) alpha, and inducible NO synthase
Mori et al., Blood 1997 (Asthma...) : NF-AT and NF-kappaB were not significantly induced by IL-2 stimulation
Krejsa et al., J Biol Chem 1997 : These results indicate a role for oxidative stress in the biological effects of some PTP inhibitors, whereas NF-kappaB activation by PTP inhibitors is mediated by mechanisms independent of intracellular redox status
Lee et al., J Immunol 1997 : In transiently transfected HUVECs, NF-kappa B-dependent gene expression was inhibited by 5-lipoxygenase inhibitors
VanArsdale et al., Proc Natl Acad Sci U S A 1997 (Adenocarcinoma) : The binding of heterotrimeric lymphotoxin, LT alpha1 beta2, to the LTbeta receptor (LTbeta R) , a member of the tumor necrosis factor receptor (TNFR) superfamily, induces nuclear factor kappaB (NF-kappaB) activation and cell death in HT29 adenocarcinoma cells
Togashi et al., Proc Natl Acad Sci U S A 1997 : Contrary to the results in Jurkat cells, PDTC did not inhibit tumor necrosis factor-alpha induced NF kappaB activation in astrocytes ; instead PDTC itself induces NF kappaB activation in astrocytes, and this may be related to scavenging of endogenously produced NO by the PDTC iron complex ... In astrocytes PDTC also dramatically induces the NF kappaB dependent enzyme, iNOS , supporting the physiologic relevance of endogenous NO regulation of NF kappaB ... Our data suggest that nNOS in astrocytes regulates NF kappaB activity and iNOS expression, and indicate a novel regulatory role for nNOS in tonically suppressing central nervous system, NF kappaB regulated genes
Tanaka et al., Biochem Biophys Res Commun 1997 (Astrocytoma) : Redox regulation of lipopolysaccharide (LPS) induced interleukin-8 (IL-8) gene expression mediated by NF kappa B and AP-1 in human astrocytoma U373 cells ... Transient expression analysis using the chloramphenicol acetyltransferase assay revealed that activation of the IL-8 promoter by LPS was stimulated by BSO and was suppressed by NAC ; likewise LPS induced activation of both NF kappa B and AP-1 was enhanced by BSO and inhibited by NAC
Sekkaï et al., Arch Biochem Biophys 1997 : This pretreatment also inhibited activation of NF-kappa B in response to TNF-alpha in lymphoblastoid J.Jhan5-1 cells
Kohler et al., Biochem Biophys Res Commun 1997 : Dexamethasone, a known inhibitor of NF-kappa B activation does not inhibit endotoxin dependent induction of I kappa B kinase suggesting that the mechanism of action of dexamethasone is different from the tolerance mechanism reported here
Ye et al., Adv Exp Med Biol 1996 : Potential significance of PAF induced NF-kappa B activation is discussed
Bierhaus et al., Thromb Haemost 1997 : The dietary pigment curcumin reduces endothelial tissue factor gene expression by inhibiting binding of AP-1 to the DNA and activation of NF-kappa B ... Curcumin inhibited TNF alpha induced I kappa B alpha degradation and the nuclear import of NF-kappa B
Whiteside et al., EMBO J 1997 : I kappa B epsilon , a novel member of the I kappa B family, controls RelA and cRel NF-kappa B activity
Chen et al., Am J Physiol 1997 : Involvement of NF-kappaB in silica induced cyclooxygenase II gene expression in rat alveolar macrophages
Iciek et al., J Immunol 1997 : In addition to the NF-kappaB/Rel complexes induced by CD40L , two nuclear complexes, each which contain both STAT6 and NF-kappaB/Rel proteins, are induced in splenic B cells by a combination of CD40L and IL-4, and bind to the CD40L/IL-4-responsive region of the germline epsilon promoter
Yurochko et al., J Virol 1997 (Cytomegalovirus Infections) : Therefore, to detail the role that Sp1 plays in the regulation of NF-kappaB during infection, we initially examined Sp1 levels for changes during infection ... Last, to further dissect the role of HCMV in the Sp1 mediated induction of NF-kappaB , we examined the role that the viral IE genes played in Sp1 regulation ... These data supported our hypothesis that Sp1 was involved in the upregulation of NF-kappaB during HCMV infection through the Sp1 binding sites in the p65 and p105/p50 promoters and additionally demonstrated a potential viral mechanism that might be responsible for the upregulation of Sp1 activity
Hsu et al., J Biol Chem 1997 : Co-expression of ATAR with TRAF5, but not TRAF2 , results in synergistic activation of NF-kappaB , suggesting potentially different roles for TRAF2 and TRAF5 in post-receptor signaling ... Co-expression of ATAR with TRAF5 , but not TRAF2, results in synergistic activation of NF-kappaB , suggesting potentially different roles for TRAF2 and TRAF5 in post-receptor signaling
Wong et al., J Clin Invest 1997 (Adenocarcinoma...) : In A549 cells induction of the stress response decreased tumor necrosis factor-alpha (TNF-alpha) mediated NF-kappaB nuclear translocation ... TNF-alpha initiates NF-kappaB nuclear translocation by causing dissociation of the inhibitory protein I-kappaBalpha from NF-kappaB and rapid degradation of I-kappaBalpha
Staak et al., Blood 1997 (Acute Disease...) : The stimulatory effect of PTX on the immediate early ( IE ) enhancer/promoter was mediated via CREB/ ATF, a eukaryotic transcription factor that binds to the 19 bp sequence motif in the enhancer region, while TNF alpha stimulation was mediated by activation of the transcription factor NF-kappaB and its binding to the 18 bp sequence motif in the enhancer
Lee et al., Biochem J 1997 : NF-kappaB dependent expression of nitric oxide synthase is required for membrane fusion of chick embryonic myoblasts ... These results suggest that NF-kappaB dependent expression of NOS is an important step in membrane fusion of chick embryonic myoblasts
Shatrov et al., Biochem Biophys Res Commun 1997 (Leukemia, Myeloid) : Moreover, we have shown that while an intracellular calcium chelator BAPTA/AM was able to inhibit both SPP- and TG-induced NF-kappa B activation, it had no effect on TNF induced NF-kappa B activation ... In addition, SPP induced NF-kappa B activation was blocked both by cyclosporin A, known to inhibit calcineurin phosphatase activity, and by the antioxidant butylated hydroxyanisole ... These observations suggest that intracellular calcium mobilization is required for SPP induced NF-kappa B activation, which may involve calcineurin- and redox dependent mechanisms
Jeon et al., Toxicol Appl Pharmacol 1997 : Conversely, NF-kappa B/Rel binding activity was inhibited on Days 2 through 4 in sRBC sensitized splenocytes in the presence of TGF-beta 1
Sekhar et al., Biochem Biophys Res Commun 1997 : Inhibition of NF-kappaB activity by overexpression of an IkappaB alpha transdominant inhibitor or exposure to the protease inhibitor TLCK did not inhibit the OA mediated increase in GLCLC transcripts
LeVan et al., Am J Physiol 1997 : The GR antagonist RU-486 inhibited the repressive effect of Dex on TNF-alpha induced NF-kappa B activity by 81 % but only counteracted the repressive effect of Dex on TPA induced AP-1 activity by 43 %
Brown et al., J Interferon Cytokine Res 1997 : Interferon-gamma (IFN-gamma) , an important regulator of macrophage gene expression, significantly enhanced NF-kappa B activity and altered the apparent molecular weight of the NF-kappa B1-like proteins in LPS stimulated and IL-2 stimulated murine macrophages ... In addition, regulation of the two pools of NF-kappa B1/p50 by IFN-gamma was confirmed by Western immunoblot analysis of cytosolic and nuclear extracts
Zhou et al., J Biol Chem 1997 (Liver Neoplasms, Experimental) : Finally, expression of either the antisense p65 subunit of NF-kappaB or dominant negative Raf-1 kinase blocked insulin 's induction of the mdr1b promoter activity
Sonoda et al., J Biol Chem 1997 : Possible role of mitogen activated protein kinase on the okadaic acid induced NF-kappaB activation
Kurebayashi et al., J Steroid Biochem Mol Biol 1997 : These findings suggested that estrogens inhibit IL-6 production by interfering with the function of NF-kappaB rather than that of NF-IL6
Kochetkova et al., J Clin Invest 1997 (Leukemia, Myelomonocytic, Chronic) : The constitutive and TNFalpha induced NF-kappaB/Rel complexes were identical and were composed mainly of p50 and c-Rel proteins ... These results indicate that the aberrant, constitutive GM-CSF gene activation in JMML is maintained by TNFalpha mediated activation of NF-kappaB/Rel proteins
Chen et al., Arch Biochem Biophys 1997 : In this report, we demonstrate that MG132, a relatively specific proteasome inhibitor, is capable of suppressing LPS induced I kappa B alpha degradation and NF-kappa B activation in mouse macrophage line RAW 264.7 cells, but is unable to influence the same induction produced by silica ... In contrast, no inhibition of LPS induced I kappa B alpha degradation and NF-kappa B activation was observed by the overexpression of calpastatin ... This suggests that calpain contributes to silica induced I kappa B alpha degradation and NF-kappa B activation but not to LPS induced I kappa B alpha degradation and NF-kappa B activation ... This suggests that calpain contributes to silica induced I kappa B alpha degradation and NF-kappa B activation but not to LPS induced I kappa B alpha degradation and NF-kappa B activation
Bourcier et al., J Biol Chem 1997 (Arteriosclerosis) : SMC cultured under serum-free conditions displayed little NFkappaB activity, but addition of serum or platelet derived growth factor did activate NFkappaB
Yurochko et al., J Virol 1997 : The human cytomegalovirus UL55 ( gB ) and UL75 ( gH ) glycoprotein ligands initiate the rapid activation of Sp1 and NF-kappaB during infection
Gamard et al., J Biol Chem 1997 : ( iii ) TNFalpha potently activated NF-kappaB in a cell line deficient in acid sphingomyelinase ... ( iv ) TNFalpha activated NF-kappaB within minutes without altering ceramide levels ... ( vi ) Ceramide generation in response to Fas activation was inhibited by N-acetyltyrosinylvalinylalanylaspartyl chloromethyl ketone, a peptide inhibitor of interleukin-1beta converting enzyme-like proteases, whereas TNFalpha induced NF-kappaB activation was unaffected by the inhibitor
Bretschneider et al., Biochem Biophys Res Commun 1997 : Stimulation of resting cells by thrombin ( 10 nM ) or TRAP ( 10-100 microM ) resulted in a comparable time dependent activation of NFkappaB as detected by Western blotting and electrophoretic mobility shift assay ( EMSA ) of nuclear extracts
O'Brien et al., Mol Cell Biol 1997 : To address the functional role of NF-kappaB in LPS and IFN-gamma induction of these events, we blocked the nuclear translocation of NF-kappaB by overexpression of a dominant negative mutant of IkappaB-alpha ( IkappaB deltaN ) ... To address the functional role of NF-kappaB in LPS and IFN-gamma induction of these events, we blocked the nuclear translocation of NF-kappaB by overexpression of a dominant negative mutant of IkappaB-alpha ( IkappaB deltaN ) ... Our results demonstrate that activation of NF-kappaB is necessary but not sufficient for LPS activation of transcription and recombination at kappa ... These results also suggest that NF-kappaB is not required for IFN-gamma activation of transcription or recombination
Lee et al., Infect Immun 1997 : Anti-Ehrlichia chaffeensis antibody complexed with E. chaffeensis induces potent proinflammatory cytokine mRNA expression in human monocytes through sustained reduction of IkappaB-alpha and activation of NF-kappaB ... Furthermore, prolonged degradation of IkappaB-alpha and activation of NF-kappaB were demonstrated in THP-1 cells exposed to anti-E
Geng et al., Free Radic Biol Med 1997 : SAC consistently exhibited a dose dependent inhibition of NF-kappa B activation induced by both TNF-alpha and H2O2
Parikh et al., J Surg Res 1997 : In other reports, the nuclear factor-kappa B (NF-kappa B) regulated IL-6 production in certain cell types ... The inhibition of IL-6 production by the NF-kappa B inhibitors indicates that the IL-6 production is regulated by NF-kappa B , although further experiments are needed to test that hypothesis
Spitsin et al., Mol Med 1997 : Human- and mouse-inducible nitric oxide synthase promoters require activation of phosphatidylcholine-specific phospholipase C and NF-kappa B ... Additionally, NF-kappa B activation is necessary but not sufficient for inducible nitric oxide synthase production in human cells, in contrast to murine cells in which it serves as a complete inducer
Horwitz et al., Immunity 1997 : These results suggest that while p50 and p65 do not regulate the maturation of pre-B cells, NF-kappaB mediates the development or survival of an early lymphocyte precursor through regulation of an extracellular factor
Bonaiuto et al., J Neuroimmunol 1997 : As expected, LPS strongly induced the formation of two NF-kappa B DNA binding activities, one of which was identified as RelA/p50
Shen et al., DNA Cell Biol 1997 : In this report, we present evidence that shows HGF can stimulate the expression of AGP/EBP ( C/EBP beta ) and NF-kappaB , which are both key transcription factors responsible for the regulation of many genes under stress conditions or during the acute-phase response ... Activation of NF-kappaB by HGF was observed to precede the induction of agp/ebp ... These results suggest that the induction of the agp/ebp gene by HGF is mediated at least in part by its activation of NF-kappaB
Stephens et al., J Autoimmun 1997 (Diabetes Mellitus, Type 1) : In the current study we have examined the effect of the same inhibitor of NF-kappaB on class I major histocompatibility complex ( MHC ) protein expression in NIT-1 cells and shown that inhibition of NF-kappaB activation decreased basal and TNF induced class I MHC levels ... Although inducible nitric oxide synthase (iNOS) may also be inhibited by inhibition of NF-kappaB , this could not be demonstrated in NIT-1/delta sp cells because wild-type NIT-1 cells express very little iNOS
Hattori et al., J Mol Cell Cardiol 1997 : Given that nuclear factor kappa B (NF-kappa B) mediates the induction of iNOS gene expression in various cell types, the role of NF-kappa B in the induction of iNOS in cytokine stimulated rat neonatal cardiac myocytes was assessed by examining the effects of pyrrolidine dithiocarbamate ( PDTC ), an inhibitor of NF-kappa B activation, on the abundance of iNOS mRNA and NO synthesis
Lo et al., J Surg Res 1997 : NF kappa B activity induced by LPS was inhibited by either BTEE or TPCK ... Inhibition of NF kappa B activity by these agents also prevented TNF mRNA expression and TNF production induced by LPS
Shattuck-Brandt et al., Cancer Res 1997 (Melanoma) : Enhanced degradation of I-kappaB alpha contributes to endogenous activation of NF-kappaB in Hs294T melanoma cells
Baud et al., Bull Acad Natl Med 1997 : Low environmental pH-induced iNOS gene expression involved the activation of nuclear factor-kappa B (NF-kappa B) transcription factor since [ 1 ] exposure of macrophages to low environmental pH increased NF-kappa B binding activity in the nucleus, and [ 2 ] treatment of macrophages with pyrrolidine dithiocarbamate or n-acetyl-leucinyl-norleucinal, two drugs preventing NF-kappa B translocation to the nucleus, canceled low pH-induced nitrite accumulation
Darnay et al., FEBS Lett 1997 : Inhibition of protein tyrosine phosphatases blocks tumor necrosis factor (TNF) induced growth modulation and NF-kappaB activation , both mediated primarily through the p60 TNF receptor ... Taken together, these results suggest that protein tyrosine phosphatases play an essential role in phosphorylation of the cytoplasmic domain of the TNF receptor and in regulation of the receptor associated kinase, and this in turn may play a role in TNF mediated growth modulation and NF-kappaB activation
van der Burg et al., Mol Hum Reprod 1996 (Inflammation) : Since suppression of inflammatory processes leading to menstruation and parturition are among the key functions of progesterone, the repression of NF-kappa B activity by the progesterone receptor may prove to be essential in the regulation of these processes
Régnier et al., Cell 1997 : Activation of the transcription factor NF-kappaB by tumor necrosis factor (TNF) and interleukin-1 (IL-1) requires the NF-kappaB inducing kinase (NIK) ... Activation of the transcription factor NF-kappaB by tumor necrosis factor (TNF) and interleukin-1 (IL-1) requires the NF-kappaB inducing kinase (NIK)
Kim et al., Biochem Biophys Res Commun 1997 : Electrophoretic mobility shift assays with the two NF-kappaB sites showed LPS induced NF-kappaB binding to both probes and its higher affinity to the upstream NF-kappaB site
Li et al., J Biol Chem 1997 : In hyperoxia, NF-kappaB regulation was independent of mitogen activated protein kinase ( MAPK )
Hauf et al., Proc Natl Acad Sci U S A 1997 (Leukemia P388...) : Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF-kappaB ( RelA/p50 ) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by IkappaBalpha and IkappaBbeta degradation ... Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF-kappaB ( RelA/p50 ) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by IkappaBalpha and IkappaBbeta degradation ... We suggest that products of the enzymatic activity of phospholipases directly interfere with host cell signal transduction pathways, thus leading to persistent NF-kappaB activation via persistent IkappaBbeta degradation
Liden et al., J Biol Chem 1997 : Glucocorticoids inhibit NF-kappaB signaling by interfering with the NF-kappaB transcription factor RelA
Greenberg et al., J Pharmacol Exp Ther 1997 : LPS increased and db-cAMP did not affect NF-kappaB in AM ... Ethanol inhibited LPS stimulated NF-kappaB
Deshpande et al., Am J Reprod Immunol 1997 : Estradiol down-regulates LPS induced cytokine production and NFkB activation in murine macrophages
Behl et al., J Neural Transm Suppl 1997 (Alzheimer Disease) : In addition, A beta induces NF-kB activity, a transcription factor thought to be regulated by oxidative stress
Koptides et al., Am J Physiol 1997 : Moreover, the NF-kappa B inhibitors blocked SP-A dependent increases in tumor necrosis factor-alpha mRNA levels
Erl et al., Am J Physiol 1997 : Electrophoretic mobility-shift assays of HUVEC nuclear proteins revealed a decrease in TNF-alpha stimulated nuclear factor-kappa B (NF-kappa B) activation after pretreatment of HUVEC with TCP succinate but not with TCP, TCP acetate, or succinate alone
Faruqi et al., Am J Physiol 1997 : Although NAC reduced nuclear factor-kappa B (NF-kappa B) activation in EC as measured by gel-shift assays using an oligonucleotide probe corresponding to the consensus NF-kappa B binding sites of the VCAM-1 gene ( VCAM-NF-kappa B ), the antioxidant had no appreciable effect when an oligomer corresponding to the consensus NF-kappa B binding site of the E-selectin gene ( E-selectin-NF-kappa B ) was used
Hong et al., J Immunol 1997 : These results indicate that the generation of H2O2 and activation of NF-kappaB by TGF-beta1 are required for the induction of M-CSF gene transcription ... These results indicate that the generation of H2O2 and activation of NF-kappaB by TGF-beta1 are required for the induction of M-CSF gene transcription
Shackelford et al., Mol Pharmacol 1997 : This observation led us to investigate the role of aspirin in suppressing the activation of the NF-kappaB regulated tumor necrosis factor-alpha (TNF-alpha) gene expression in primary macrophages
Chu et al., Proc Natl Acad Sci U S A 1997 : When overexpressed in mammalian cells, c-IAP2 activates NF-kappaB and suppresses TNF cytotoxicity ... In contrast to wild-type c-IAP2, a mutant lacking the C-terminal RING domain inhibits NF-kappaB induction by TNF and enhances TNF killing
Hwang et al., Biochem Pharmacol 1997 : Inhibitors of NF-kappaB suppressed COX-2 expression without affecting tyrosine phosphorylation of MAPKs ... Together, these results indicate that the activation of NF-kappaB is required to induce the expression of COX-2 in LPS stimulated RAW 264.7 cells
De Valck et al., Biochem Biophys Res Commun 1997 : However, A20 mutants that no longer associated with 14-3-3 proteins could still fully inhibit NF-kappaB activation induced by tumor necrosis factor, interleukin-1beta or phorbol 12-myristate 13-acetate, thus excluding a crucial role for 14-3-3 interaction in this A20 function ... However, A20 mutants that no longer associated with 14-3-3 proteins could still fully inhibit NF-kappaB activation induced by tumor necrosis factor , interleukin-1beta or phorbol 12-myristate 13-acetate, thus excluding a crucial role for 14-3-3 interaction in this A20 function ... However, A20 mutants that no longer associated with 14-3-3 proteins could still fully inhibit NF-kappaB activation induced by tumor necrosis factor, interleukin-1beta or phorbol 12-myristate 13-acetate, thus excluding a crucial role for 14-3-3 interaction in this A20 function
Timmerman et al., J Immunol 1997 : Additionally, Ab interaction with NF-ATp induces high affinity NF-kappaB site interaction, suggesting that nuclear partners may dramatically vary the specificity of the NF-AT family
Heck et al., EMBO J 1997 (Inflammation) : It has been proposed that the glucocorticoid dependent inhibition of NF-kappaB activity is mediated by increased synthesis of I kappaB alpha which should then sequester NF-kappaB in an inactive cytoplasmic form ... Here, we show by the use of a mutant glucocorticoid receptor and steroidal ligands that hormone induced I kappaB alpha synthesis and inhibition of NF-kappaB activity are separable biochemical processes
Finco et al., J Biol Chem 1997 (Cell Transformation, Neoplastic) : Oncogenic Ha-Ras induced signaling activates NF-kappaB transcriptional activity, which is required for cellular transformation ... We show here that oncogenic forms of Ha-Ras activate NF-kappaB , not through induced nuclear translocation, but rather through the activation of the transcriptional function of the NF-kappaB RelA/p65 subunit
Mori et al., Eur J Haematol 1997 (Lymphoma, T-Cell...) : Exogenous TNF-alpha and the monoclonal antibody to TNF-alpha did not affect IL-10 production and constitutive NF-kappa B binding levels in HuT 78 cells
Sontag et al., EMBO J 1997 : Constitutive activation of PKC zeta and NF-kappaB following inhibition of PP2A supports new mechanisms by which SV40 small t promotes cell growth and transformation
Franchimont et al., J Clin Invest 1997 (Bone Resorption) : 5 ' deletions of the IL-6 promoter and targeted mutations of the multiple response element ( MRE ) /cAMP responsive element ( CRE ), the nuclear factor for IL-6 ( NF-IL-6 ), and the nuclear factor-kappaB (NF-kappaB) binding sites indicated that NF-IL-6 and NF-kappaB , in combination with MRE/CRE, binding sites are required for the induction of the IL-6 promoter by IL-6
Gukovskaya et al., J Clin Invest 1997 (Disease Models, Animal...) : TNFalpha potentiated NF-kappaB translocation into the nucleus and stimulated apoptosis in isolated acini while not affecting LDH release
Min et al., J Immunol 1997 : Transient overexpression of the TRAF2 adaptor protein can activate NF-kappaB and endogenous JNK, whereas N-terminal truncated TRAF2 protein blocks TNF induced NF-kappa B and JNK activation as well as E-selectin promoter-reporter gene transcription ... Transient overexpression of the TRAF2 adaptor protein can activate NF-kappaB and endogenous JNK, whereas N-terminal truncated TRAF2 protein blocks TNF induced NF-kappa B and JNK activation as well as E-selectin promoter-reporter gene transcription ... These data suggest that TNF activates parallel TRAF-NF-kappa B and TRAF-RAC/CDC42-JNK-c-Jun/ATF2 pathways to initiate E-selectin transcription
Pollack et al., J Immunol 1997 (Inflammation) : The modulation by anti-LPS mAbs of mCD14 mediated LPS uptake was associated with inhibition of LPS induced nuclear factor-kappaB (NF-kappaB) translocation and TNF-alpha secretion in CHO-CD14 cells and monocytes, respectively, while mAb enhancement of non-mCD14 mediated LPS uptake stimulated these activities
Kacimi et al., Circulation 1997 (Anoxia...) : Radioligand binding and electrophoretic mobility shift assays showed that during chronic hypoxia, IL-1 receptor density and activity of the transcription factor NF-kappaB induced by IL-1beta were decreased, which may account at least in part for the decrease in iNOS expression
Bowie et al., J Biol Chem 1997 : Lipid peroxidation is involved in the activation of NF-kappaB by tumor necrosis factor but not interleukin-1 in the human endothelial cell line ECV304 ... Here we have investigated the effect of a range of putative antioxidants on NF-kappaB activation by interleukin-1 and tumor necrosis factor as well as the ability of H2O2 to activate NF-kappaB in primary human umbilical vein endothelial cells and the transformed human endothelial cell line ECV304 ... Here we have investigated the effect of a range of putative antioxidants on NF-kappaB activation by interleukin-1 and tumor necrosis factor as well as the ability of H2O2 to activate NF-kappaB in primary human umbilical vein endothelial cells and the transformed human endothelial cell line ECV304 ... Finally, butylated hydroxyanisole, which inhibits lipid peroxidation but has no iron chelating properties, inhibited NF-kappaB activation by tumor necrosis factor but not interleukin-1
Gringhuis et al., Blood 1997 (Leukemia-Lymphoma, Adult T-Cell) : T-cell receptor (TCR)/CD3 engagement results in the activation of nuclear factor of activated T cells ( NFAT ), activator protein-1 (AP-1), and nuclear factor kappaB (NFkappaB) , whereas the CD28 responsive complex (CD28RC) is activated in response to the CD28 signal ... T-cell receptor (TCR)/CD3 engagement results in the activation of nuclear factor of activated T cells ( NFAT ), activator protein-1 (AP-1), and nuclear factor kappaB (NFkappaB) , whereas the CD28 responsive complex (CD28RC) is activated in response to the CD28 signal
Chao et al., Neuroreport 1997 : In the present study using primary human fetal astrocyte cultures, we found that IL-1 beta stimulated activation of nuclear factor kappa B (NF-kappa B) within 2 h, iNOS mRNA expression at 8 h, and maximal NO production by 5 days post-treatment
Clemens et al., Brain Res Mol Brain Res 1997 (Brain Ischemia) : The oxidative stress responsive transcription factor nuclear factor-kappa B (NF-kappa B) consists of a p50 ( 50 kDa ) and p65/RelA ( 65 kDa ) component and can be activated in vitro by TNF alpha , IL1 beta, hydrogen peroxide and oxygen radicals ... The oxidative stress responsive transcription factor nuclear factor-kappa B (NF-kappa B) consists of a p50 ( 50 kDa ) and p65/RelA ( 65 kDa ) component and can be activated in vitro by TNF alpha, IL1 beta , hydrogen peroxide and oxygen radicals
Bierhaus et al., Circulation 1997 : Binding of advanced glycation end products ( AGEs ) to the cellular surface receptor ( RAGE ) induces translocation of the transcription factor NF-kappaB into the nucleus and NF-kappaB mediated gene expression ... Electrophoretic mobility shift assays and Western blot analysis demonstrated that the AGE albumin induced translocation of NF-kappaB from the cytoplasm into the nucleus was suppressed in the presence of antisense RAGE but not by sense RAGE ... Electrophoretic mobility shift assays and Western blot analysis demonstrated that the AGE albumin induced translocation of NF-kappaB from the cytoplasm into the nucleus was suppressed in the presence of antisense RAGE but not by sense RAGE
Marumo et al., Circulation 1997 : Gel mobility shift assay revealed that PDGF-AB increased the binding activity of NF-kappaB, which contained predominantly the p50/p65 heterodimer in nuclear extracts from HSMCs. Superoxide dismutase as well as iodonium diphenyl, Ro 31-8220, and wortmannin attenuated PDGF-AB induced activation of NF-kappaB and expression of MCP-1 mRNA ... In contrast, superoxide dismutase did not inhibit the interleukin-1beta induced NF-kappaB activation ... In contrast, superoxide dismutase did not inhibit the interleukin-1beta induced NF-kappaB activation ... This PDGF induced O2.- production may be involved in vascular lesion formation by mediating, at least in part, NF-kappaB activation and MCP-1 induction
Makarov et al., Gene Ther 1997 : We evaluated the feasibility of suppressing the inflammatory responses in different cell lines through specific inhibition of NF-kappa B by gene transfer of I kappa B alpha , the naturally occurring intracellular inhibitor of NF-kappa B ... The effects of the I kappa B alpha overexpression on the NF-kappa B activation and the inflammatory responses varied significantly in different cell lines ... In conditionally immortalized human endometrial stromal cells, overexpression of I kappa B alpha prevented both interleukin-1 (IL-1)-inducible degradation of endogenous I kappa B alpha protein and activation of NF-kappa B ... In conditionally immortalized human endometrial stromal cells, overexpression of I kappa B alpha prevented both interleukin-1 (IL-1)-inducible degradation of endogenous I kappa B alpha protein and activation of NF-kappa B
Recio et al., J Biol Chem 1997 : NGF does not activate NF-kappaB in PC12 cells, but rather increases binding of other nuclear factors to the kappaB sequences
Dhawan et al., Eur J Immunol 1997 : Recent studies from our laboratory have indicated that protein tyrosine phosphatase ( PTPase ) inhibitors can down-modulate the tumor necrosis factor (TNF) mediated activation of the nuclear transcription factor NF-kappa B in ML-1a, a monocytic cell line ( Singh and Aggarwal, J. Biol. Chem. 1995 : 270 : 10631 ) ... Besides PAO, other inhibitors of PTPase, including pervanadate and diamide, also blocked TNF dependent NF-kappa B activation and induction of all the three adhesion proteins
Cheshire et al., Mol Cell Biol 1997 : Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta degradation ... Synergistic activation of NF-kappaB by tumor necrosis factor alpha and gamma interferon via enhanced I kappaB alpha degradation and de novo I kappaBbeta degradation ... Typically, the synergistic effect on gene expression is due to the independent activation of nuclear factor kappaB (NF-kappaB) by TNF-alpha and of signal transducers and activators of transcription or IFN-regulatory factor 1 by IFNs, allowing these transcription factors to bind their unique promoter sites ... However, since activation of NF-kappaB by TNF-alpha is often transient and would not activate long-term kappaB dependent transcription effectively, we explored the effects of IFN-gamma on TNF-alpha induced NF-kappaB activity ... However, since activation of NF-kappaB by TNF-alpha is often transient and would not activate long-term kappaB dependent transcription effectively, we explored the effects of IFN-gamma on TNF-alpha induced NF-kappaB activity ... IFN-gamma, which typically does not activate NF-kappaB, synergistically enhanced TNF-alpha induced NF-kappaB nuclear translocation via a mechanism that involves the induced degradation of I kappaBbeta and that apparently requires tyrosine kinase activity in preneuronal cells but not in endothelial cells ... IFN-gamma , which typically does not activate NF-kappaB, synergistically enhanced TNF-alpha induced NF-kappaB nuclear translocation via a mechanism that involves the induced degradation of I kappaBbeta and that apparently requires tyrosine kinase activity in preneuronal cells but not in endothelial cells
Kupatt et al., J Mol Cell Cardiol 1997 : Because upregulation of AP-1 through hypoxia alone did not affect ICAM-1 expression, we conclude that redox-sensitive NFkappaB activation triggers ICAM-1 upregulation
Kelly et al., Biochem Biophys Res Commun 1997 (Breast Neoplasms) : MCP-1 synthesis is stimulated by the transcription factor NF-kappa B and is inhibited by glucocorticoid but the relevance of progesterone control in reproductive tissue is unknown
Woronicz et al., Science 1997 : IkappaB kinase-beta : NF-kappaB activation and complex formation with IkappaB kinase-alpha and NIK ... IkappaB kinase-beta (IKK-beta) activated NF-kappaB when overexpressed and phosphorylated serine residues 32 and 36 of IkappaB-alpha and serines 19 and 23 of IkappaB-beta ... Overexpression of a catalytically inactive form of IKK-beta blocked cytokine induced NF-kappaB activation
Hofmeister et al., J Biol Chem 1997 : In this cell line ( EL4D6/76 ), tumor necrosis factor induced ligand/receptor internalization, NFkappaB nuclear translocation, IL-2 production, and the activation of neutral (N)-SMase and acid ( A ) -SMase
Hishikawa et al., Circ Res 1997 : In electrophoretic mobility shift assays, tumor necrosis factor-alpha activated nuclear factor-kappa B (NF-kappa B) with a peak at approximately 3 hours, whereas pulsatile stretch showed sustained activation during stimulation for up to 24 hours
Yaron et al., EMBO J 1997 : Microinjection of the inhibitory peptides into stimulated cells abolished NF-kappa B activation in response to TNF alpha and the consequent expression of E-selectin, an NF-kappa B-dependent cell-adhesion molecule
Roshak et al., J Pharmacol Exp Ther 1997 (Arthritis, Rheumatoid) : Direct action of hymenialdisine on IL-1 induced NFkappaB activation was demonstrated by a significant reduction ( approximately 80 % ) in NFkappaB binding to the classical kappaB consensus motif ( as assessed by electrophoretic mobility shift assay ) and inhibition of stimulated p65 migration from the cytosol of treated cells ( as assessed by Western analysis ) ... Consistent with the role of NFkappaB in the transcriptional regulation of COX II and 85-kDa PLA2 , hymenialdisine treated RSF did not transcribe the respective mRNAs in response to IL-1
Robinson et al., Immunity 1997 : IL-1alpha had no effect on proliferation, cytokine production, or NFkappaB activation in Th1 cells but activated NFkappaB and proliferation in Th2 cells
Izumi et al., Proc Natl Acad Sci U S A 1997 (Cell Transformation, Neoplastic...) : In transfection assays, TRADD and TES2 synergistically mediated high-level NF-kappaB activation ... In transfection assays, TRADD and TES2 synergistically mediated high-level NF-kappaB activation
Khachigian et al., Am J Pathol 1997 : Inducible vascular cell adhesion molecule-1 ( VCAM-1 ) in glomerular mesangial cells ( GMC ) exposed to lipopolysaccharide (LPS) in vitro involves the activation of nuclear factor-kappa B (NF-kappa B) and its interaction with the proximal VCAM-1 promoter
Brigelius-Flohé et al., Biochem J 1997 : To test the involvement of hydroperoxides we investigated whether IL-1 induced NF kappa B activation could be influenced by glutathione peroxidases ( GPx ) ... IL-1 induced NF kappa B activation was inhibited by selenium supplementation in control cells ... In ECVPHGPx+SelD+ under conditions of selenium restriction, IL-1 induced NF kappa B activation only to a similar extent as under conditions of selenium supplementation in controls, and activation was abolished with 50 nM sodium selenite ... These results show that overexpressed PHGPx is sufficient to inhibit NF kappa B activation, and suggests that NF kappa B activation by IL-1 is mediated by a preferential substrate of PHGPx, such as a fatty acid hydroperoxide, rather than by H2O2, the preferred substrate of the more abundant cytosolic GPx ... These results show that overexpressed PHGPx is sufficient to inhibit NF kappa B activation, and suggests that NF kappa B activation by IL-1 is mediated by a preferential substrate of PHGPx, such as a fatty acid hydroperoxide, rather than by H2O2, the preferred substrate of the more abundant cytosolic GPx
Bendall et al., J Biol Chem 1997 : Transcription factor NF-kappaB regulates inducible Oct-2 gene expression in precursor B lymphocytes
Reddy et al., J Biol Chem 1997 : Furthermore, two PI 3-kinase-specific inhibitors, wortmannin and a dominant negative mutant of the p85 subunit, inhibited IL-1 induced activation of both NFkappaB and AP-1 ... Furthermore, two PI 3-kinase-specific inhibitors, wortmannin and a dominant negative mutant of the p85 subunit, inhibited IL-1 induced activation of both NFkappaB and AP-1 ... Our results thus indicate that PI 3-kinase is a novel signal transducer in IL-1 signaling and that it may differentially mediate the activation of NFkappaB and AP-1
Floettmann et al., Oncogene 1997 : These inducible chimeric proteins also allowed us to demonstrate that LMP1 mediated NF-kappaB activation is an immediate event following activation of LMP1
Luan et al., J Leukoc Biol 1997 (Disease Progression...) : We propose that this endogenous nuclear NF-kappaB , working in concert with the 115-kDa IUR binding factor, promotes constitutive expression of MGSA/GRO genes
Visconti et al., Oncogene 1997 (Thyroid Neoplasms) : Our results indicate that activation of the NFkappaB complex by overexpression of p65 plays a critical role in the process of thyroid cell transformation
Hsing et al., J Immunol 1997 (Lymphoma, B-Cell) : This suggests that in B cells, CD40 induced NF-kappa B activation can occur independently of TRAF2 and TRAF5 association ... Proteasome dependent inhibitory kappa B alpha ( I kappa B alpha ) and I kappa B beta degradation occurred downstream of CD40 ligation and preceded CD40 mediated NF-kappa B nuclear translocation
Lentsch et al., J Clin Invest 1997 : Transcriptional control of the TNF alpha gene is regulated by the nuclear factor kappa B (NF-kappa B) ... Activation of NF-kappa B involves the degradation of its cytoplasmic inhibitor I kappa B alpha , allowing the nuclear translocation of NF-kappa B, with ensuing transcriptional activation ... In this study, we sought to determine whether the protective effects of IL-10 and IL-13 in IgG immune complex induced lung injury were mediated by inhibition of NF-kappa B activation ... In this study, we sought to determine whether the protective effects of IL-10 and IL-13 in IgG immune complex induced lung injury were mediated by inhibition of NF-kappa B activation
Taylor et al., Arch Surg 1997 : These data indicate that NO down-regulates iNOS gene transcription, and that the effect is mediated in part by inhibiting NF-kappa B activity
Kundu et al., J Biol Chem 1997 : In vitro transcription assay demonstrates that E2F-1 represses NF-kappaB mediated transcription in a cell-free system
Chen et al., J Virol 1997 : Given the known roles of LT-betaR/LT-alpha1,beta2 receptor-ligand interactions in the normal development of peripheral lymphoid organs and in triggering cytolytic activity and NF-kappaB activation in certain cell types, our finding implies that the HCV core protein may aggravate these biological functions of LT-betaR, resulting in pathogenesis in HCV infected cells
Yu et al., Biochem J 1997 (Hypotension...) : Moreover electrophoretic mobility-shift assays demonstrated that PPM-18 inhibited DNA binding to NF-kappaB induced by LPS in whole cells but not when added in the nuclear extract, suggesting that PPM-18 did not interfere directly with the binding of NF-kappaB to DNA and that some events had to be processed before NF-kappaB could bind DNA ... Therefore the stabilization of IkappaBalpha might have contributed to the inhibition of NF-kappaB activation
Ray et al., Biochem J 1997 : Evidence that GCs also inhibited cytokine activation of a synthetic nuclear factor kappaB (NFkappaB)-driven reporter gene transiently transfected into A549 cells suggested that interference with the activation and/or function of this transcription factor was important for GC inhibition of ESAP
Huang et al., Proc Natl Acad Sci U S A 1997 : Overexpression of an IL-1RAcP mutant lacking its intracellular domain, the IRAK binding domain, prevented the recruitment of IRAK to the receptor complex and blocked IL-1 induced NF-kappaB activation ... Overexpression of an IL-1RAcP mutant lacking its intracellular domain, the IRAK binding domain, prevented the recruitment of IRAK to the receptor complex and blocked IL-1 induced NF-kappaB activation
Jornot et al., FEBS Lett 1997 : Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation , and no induction of NFkappaB binding activity ... In contrast, cells exposed to TNF alpha showed enhanced CREP binding activity, activation of NFkappaB and no induction of HSE-HSF complex
Nathens et al., Am J Respir Cell Mol Biol 1997 (Respiratory Distress Syndrome, Newborn) : The effect of in vivo treatment with PDTC on LPS induced NF-kappaB DNA binding activity in macrophage nuclear extracts was evaluated with the electrophoretic mobility shift assay ( EMSA )
Muzio et al., Science 1997 : Two additional proximal mediators were identified that are required for IL-1R induced NF-kappaB activation : IRAK-2, a Pelle family member, and MyD88, a death domain containing adapter molecule ... Dominant negative forms of either attenuate IL-1R mediated NF-kappaB activation
Chaturvedi et al., J Biol Chem 1997 : As sanguinarine also inhibited NF-kappaB activation induced by interleukin-1 , phorbol ester, and okadaic acid but not that activated by hydrogen peroxide or ceramide, the pathway leading to NF-kappaB activation is likely different for different inducers
Morales et al., J Biol Chem 1997 : Despite activation of nuclear factor-kappaB (NF-kappaB) by TNF , this transcription factor was not required for TNF induced transcription of gamma-GCS-HS as revealed by deletion constructs of the gamma-GCS-HS promoter subcloned in a chloramphenicol acetyltransferase reporter vector and transfected into HepG2 cells
Sica et al., J Biol Chem 1997 : Based on this observation, we have analyzed the potential role of NF-kappaB and NFAT family members in regulating IFN-gamma transcription
Benoliel et al., J Cell Sci 1997 : These compounds inhibited the insulin induced nuclear translocation of NF-kappa B as detected by confocal laser scanning microscopy
Chen et al., Mol Pharmacol 1997 : Transfection with a plasmid containing cDNA encoding mouse IkappaBbeta , an inhibitor of NF-kappaB , resulted in increased toxicity by menadione
Schmidt et al., Chem Biol 1995 (Second Messenger Systems) : Cell lines stably overexpressing the H2O2 degrading enzyme catalase were deficient in activating NF-kappa B in response to tumor necrosis factor alpha (TNF) or okadaic acid ... The catalase inhibitor aminotriazol restored NF-kappa B induction ... In contrast, stable overexpression of cytoplasmic Cu/Zn dependent superoxide dismutase ( SOD ) , which enhances the production of H2O2 from superoxide, potentiated NF-kappa B activation ... Our data show that one ROI species, H2O2 acts as a messenger in the TNF- and okadaic acid induced post-translational activation of NF-kappa B
Spiecker et al., J Biol Chem 1997 : The induction of vascular cell adhesion molecule-1 ( VCAM-1 ) expression by tumor necrosis factor (TNF)-alpha requires the activation of nuclear factor-kappaB (NF-kappaB) via a process involving the phosphorylation and degradation of its cytoplasmic inhibitor, IkappaBalpha ... The induction of vascular cell adhesion molecule-1 ( VCAM-1 ) expression by tumor necrosis factor (TNF)-alpha requires the activation of nuclear factor-kappaB (NF-kappaB) via a process involving the phosphorylation and degradation of its cytoplasmic inhibitor, IkappaBalpha ... Activation of NF-kappaB by TNF-alpha occurred within 15 min and coincided with rapid degradation of IkappaBalpha ... These findings indicate that NO inhibits NF-kappaB activation and VCAM-1 expression by increasing the expression and nuclear translocation of IkappaBalpha
Ueda et al., J Biol Chem 1997 : DNase I footprinting revealed that LPS stimulation induced protein binding to the two closely located NF-kappaB sites, A1 and A2
Lee et al., Immunity 1997 (Hypertrophy...) : TRAF2 is essential for JNK but not NF-kappaB activation and regulates lymphocyte proliferation and survival
Hamdane et al., Oncogene 1997 : Activation of p65 NF-kappaB protein by p210BCR-ABL in a myeloid cell line ( P210BCR-ABL activates p65 NF-kappaB ) ... Activation of p65 NF-kappaB protein by p210BCR-ABL in a myeloid cell line ( P210BCR-ABL activates p65 NF-kappaB ) ... Activation of p65 NF-kappaB protein by p210BCR-ABL in a myeloid cell line ( P210BCR-ABL activates p65 NF-kappaB ) ... We have analysed the effect of p210BCR-ABL expression on NF-kappaB activity in DA1 cells ( an IL-3 dependent murine myeloid progenitor cell line ) ... We have analysed the effect of p210BCR-ABL expression on NF-kappaB activity in DA1 cells ( an IL-3 dependent murine myeloid progenitor cell line ) ... We have analysed the effect of p210BCR-ABL expression on NF-kappaB activity in DA1 cells ( an IL-3 dependent murine myeloid progenitor cell line ) ... A specific stimulation of NF-kappaB activity by kinase-active wild-type p210BCR-ABL has been evidenced by transcriptional activation assays ... A specific stimulation of NF-kappaB activity by kinase-active wild-type p210BCR-ABL has been evidenced by transcriptional activation assays ... A specific stimulation of NF-kappaB activity by kinase-active wild-type p210BCR-ABL has been evidenced by transcriptional activation assays
Im et al., Eur J Immunol 1997 : The activation of NF-kappa B by PAF preceded the LPS mediated TNF-alpha gene expression ... These data suggest that PAF, which is released immediately or shortly after LPS injection, induces the expression of TNF-alpha through the activation of NF-kappa B ... These data suggest that PAF , which is released immediately or shortly after LPS injection, induces the expression of TNF-alpha through the activation of NF-kappa B
Tewes et al., Eur J Immunol 1997 (Thymoma...) : We therefore investigated whether the activation of the IL-1RI associated protein kinase could be a target for redox regulation and whether an altered activity of the kinase could influence IL-1 mediated NF-kappa B activation
Kitajima et al., Rinsho Byori 1997 (Arthritis...) : We think activation of NF-kappa B by Tax is important for tumor progression
Kinugawa et al., Circ Res 1997 : These data suggest that ( 1 ) iNOS induction simultaneously requires both NF-kappa B activation and IRF-1 induction , and ( 2 ) the heterodimer between C/EBP and CREB has synergistic effects on the iNOS induction via the CAAT box ... These data suggest that ( 1 ) iNOS induction simultaneously requires both NF-kappa B activation and IRF-1 induction, and ( 2 ) the heterodimer between C/EBP and CREB has synergistic effects on the iNOS induction via the CAAT box
Faux et al., Environ Health Perspect 1997 : This would suggest that lipoxygenase metabolites of arachidonic acid, produced following lipid peroxidation, are involved in the cellular signalling events to NF-kappa B transcription factor induction by asbestos
Adams et al., J Leukoc Biol 1997 : As expected, lipopolysaccharide (LPS) activated p65/p50 NF-kappaB and C/EBP-beta NF-IL-6 complexes, increased the nuclear titer of p65 and p50 antigens, and increased cytokine ( IL-1beta, tumor necrosis factor alpha ) mRNA production
Jope et al., Brain Res Mol Brain Res 1997 (Neuroblastoma) : Besides being differentially sensitive to inhibition by lithium, activation of AP-1 and NF-kappaB demonstrated different carbachol EC50 concentrations, and carbachol induced activation of AP-1 , but not NF-kappaB, was inhibited by treating cells with Ni2+, which blocks receptor mediated calcium influx
Yeh et al., DNA Cell Biol 1997 (Cell Transformation, Viral) : In this study, we tested if this 10-amino-acid deletion affected the induction of NF-kappaB activity by LMP 1
Bundy et al., J Biol Chem 1997 : Diverse effects of BCL3 phosphorylation on its modulation of NF-kappaB p52 homodimer binding to DNA
Klahr et al., Kidney Int Suppl 1997 (Kidney Diseases...) : ACE inhibition, AT-1 and AT-2 receptor blockade each differentially attenuated NF-kappa B isotype activation ... ACE inhibition, AT-1 and AT-2 receptor blockade each differentially attenuated NF-kappa B isotype activation ... ACE inhibition, AT-1 and AT-2 receptor blockade each differentially attenuated NF-kappa B isotype activation
Ferrari et al., J Cell Biol 1997 : Here, we investigated the effect of P2Z receptor ligation on the activation of NF-kappaB , a transcription factor controlling cytokine expression and apoptosis
Newton et al., FEBS Lett 1997 : Furthermore, equivalent effects were observed on NF-kappaB binding to COX-2 promoter kappaB sites and activation of the Jun N-terminal kinases (JNK), p54 and p46 ... Furthermore, equivalent effects were observed on NF-kappaB binding to COX-2 promoter kappaB sites and activation of the Jun N-terminal kinases (JNK), p54 and p46 ... Furthermore, equivalent effects were observed on NF-kappaB binding to COX-2 promoter kappaB sites and activation of the Jun N-terminal kinases (JNK) , p54 and p46
D'Acquisto et al., FEBS Lett 1997 : Involvement of NF-kappaB in the regulation of cyclooxygenase-2 protein expression in LPS stimulated J774 macrophages ... We investigated the involvement of NF-kappaB in the regulation of COX-2 protein expression and prostaglandin production in LPS stimulated J774 macrophages ... Moreover, APDC and TLCK both inhibited the LPS induced increase of NF-kappaB DNA binding activity and prevented IkappaB-alpha degradation ... Our results show for the first time that NF-kappaB is involved in COX-2 protein expression in LPS stimulated J774 macrophages and suggest that inhibitors of NF-kappaB activation may represent a useful tool for the pharmacological control of inflammation
Jeong et al., Immunology 1997 : Tosylphenylalanine chloromethyl ketone inhibits TNF-alpha mRNA synthesis in the presence of activated NF-kappa B in RAW 264.7 macrophages ... Our results show that TPCK inhibits LPS induced TNF-alpha mRNA synthesis in the presence of activated NF-kappa B and suggests that mechanisms other than NF-kappa B activation are involved in the transcriptional regulation of the TNF-alpha gene
Herrmann et al., Exp Cell Res 1997 (Prostatic Neoplasms) : Expression of bcl-2 did not impose a block to, or potentiate, TNF-alpha signaling of I kappa B alpha degradation, nuclear import of the RelA p65, or NF-kappa B-dependent transactivation
Miyamoto et al., Mol Cell Biol 1998 : We observed that IkappaB beta , another NF-kappaB inhibitor , is also complexed with c-Rel but slowly degraded by a proteasome dependent process in WEHI231 cells
Schwenger et al., Mol Cell Biol 1998 : Activation of NF-kappaB by TNF and IL-1 is initiated by the phosphorylation of the inhibitory subunit, IkappaB, which targets IkappaB for degradation and leads to the release of active NF-kappaB ... Activation of NF-kappaB by TNF and IL-1 is initiated by the phosphorylation of the inhibitory subunit, IkappaB, which targets IkappaB for degradation and leads to the release of active NF-kappaB ... The nonsteroidal anti-inflammatory drug sodium salicylate ( NaSal ) interferes with TNF induced NF-kappaB activation by inhibiting phosphorylation and subsequent degradation of the IkappaB alpha protein ... Recent evidence indicated that NaSal activates the p38 mitogen activated protein kinase ( MAPK ), raising the possibility that inhibition of NF-kappaB activation by NaSal is mediated by p38 MAPK
Attar et al., Mol Cell Biol 1998 : These results show that in nonstimulated T cells, IkappaB beta can not efficiently displace IkappaB alpha bound to RelA containing complexes and that persistent NF-kappaB activity is required for proper T-cell responses in vivo ... These results show that in nonstimulated T cells, IkappaB beta can not efficiently displace IkappaB alpha bound to RelA containing complexes and that persistent NF-kappaB activity is required for proper T-cell responses in vivo
Rockey et al., Hepatology 1998 : Finally, inhibitors of NF-kappaB blocked HA fragment dependent iNOS mRNA induction in Kupffer and sinusoidal endothelial cells
Bachelerie et al., J Cell Sci 1997 : Furthermore, in cells pulsed with TNF, a treatment which favors nuclear accumulation of newly synthesized IkappaBalpha, NLS-PK-NES expression promoted sustained accumulation of nuclear NF-kappaB lacking DNA binding activity ... This NES mediated accumulation of inactive nuclear NF-kappaB is likely the consequence of interference in the IkappaBalpha mediated export of NF-kappaB
Salzman et al., Gastroenterology 1998 (Salmonella Infections) : In DLD-1 cells, S. dublin mediated NOx was blocked by inhibitors of nuclear factor kappa B (NF-kappa B) and tyrosine kinase activation and was steroid resistant
Degli-Esposti et al., Immunity 1997 : The novel receptor TRAIL-R4 induces NF-kappaB and protects against TRAIL mediated apoptosis, yet retains an incomplete death domain
Chaudhary et al., Immunity 1997 : However, contrary to the previous reports, both DR4- and DR5 induced apoptosis can be blocked by dominant negative FADD , and both receptors can activate NF-kappaB using a TRADD dependent pathway
Schneider et al., Immunity 1997 : TRAIL receptors 1 ( DR4 ) and 2 ( DR5 ) signal FADD dependent apoptosis and activate NF-kappaB
Wesche et al., Immunity 1997 : The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-kappaB activation induced by IL-1, but not by TNF ... The C-terminus of MyD88 interacts with the IL-1 receptor and blocks NF-kappaB activation induced by IL-1 , but not by TNF
Hehner et al., J Biol Chem 1998 : Sesquiterpene lactones specifically inhibit activation of NF-kappa B by preventing the degradation of I kappa B-alpha and I kappa B-beta ... Sesquiterpene lactones specifically inhibit activation of NF-kappa B by preventing the degradation of I kappa B-alpha and I kappa B-beta ... Here we show that SLs prevented the activation of NF-kappa B by different stimuli such as phorbol esters, tumor necrosis factor-alpha, ligation of the T-cell receptor, and hydrogen peroxide in various cell types
Kashiwada et al., J Exp Med 1998 : CD40 activates nuclear factor kappa B (NF kappa B) and the mitogen activated protein kinase ( MAPK ) subfamily, including extracellular signal regulated kinase ( ERK ) ... Here we report that transient expression of TRAF6 stimulated both ERK and NF kappa B activity in the 293 cell line
Borrello et al., Arch Biochem Biophys 1997 : NF kappa B-independent transcriptional induction of the human manganous superoxide dismutase gene
Brophy et al., Immunogenetics 1998 (Lymphoma, B-Cell) : B cells and macrophages both activate NF-kappaB/Rel in response to lipopolysaccharide (LPS) , but differ in sensitivity to LPS and in downstream genes that are activated
Costas et al., Medicina (B Aires) 1997 : In TNF-alpha and GC target cell lines, it was found that : 1 ) TNF-alpha enhanced GR number in L-929 cells, and 2 ) by transfection of these cells with a reporter plasmid carrying the GR promoter, that TNF-alpha induced increase in GR is at the transcriptional level, 3 ) by electrophoretic mobility shift assay, using nuclear extracts of TNF-alpha ( 0.02 ng/ml ) or TNF-alpha plus DEX ( 10 nM ) stimulated L-929 cells, that cytokines can increase the binding of GR to GRE ( 45 min, 1.8 x ), while the TNF-alpha induced NFkB factor expression was not affected by GC
De Vera et al., Am J Physiol 1997 : Dexamethasone suppresses iNOS gene expression by upregulating I-kappa B alpha and inhibiting NF-kappa B ... Cytokine stimulated inducible nitric oxide synthase (iNOS) gene expression is dependent on nuclear factor-kappa B (NF-kappa B) activation and is suppressed by glucocorticoids ( GC )
Pahan et al., Free Radic Biol Med 1998 : Since activation of NF-kappaB is necessary for the induction of iNOS , we examined the effect of NAC on the activation of NF-kappaB ... Since activation of NF-kappaB is necessary for the induction of iNOS, we examined the effect of NAC on the activation of NF-kappaB ... Inhibition of LPS induced activation of NF-kappaB by NAC in rat peritoneal macrophages suggests that the inhibitory effect of NAC on the induction of iNOS is due to the inhibition of NF-kappaB ... Inhibition of LPS induced activation of NF-kappaB by NAC in rat peritoneal macrophages suggests that the inhibitory effect of NAC on the induction of iNOS is due to the inhibition of NF-kappaB
Kessler et al., J Cardiovasc Pharmacol 1997 : Thiopental did not affect the IL-1beta stimulated activation of NF-kappaB in VSMCs
Muñoz-Fernández et al., J Allergy Clin Immunol 1997 : Anti-TNF-alpha also prevented nuclear factor-kappa B (NF-kappa B) activation, and a good correlation between this inhibition and inhibition of HIV replication was observed ... Moreover, supplementing the cultures with high doses of IL-2 reverted anti-TNF-alpha inhibition of cell proliferation but did not affect the inhibition of HIV p24 antigen release or NF-kappa B activation in the same cultures ... Moreover, supplementing the cultures with high doses of IL-2 reverted anti-TNF-alpha inhibition of cell proliferation but did not affect the inhibition of HIV p24 antigen release or NF-kappa B activation in the same cultures
Dixon et al., Brain Res 1997 (Ischemic Attack, Transient) : In this study, we show that NF-kB p50 and NF-kB p65 act in synergy to transactivate the bcl-x promoter in co-transfected 293 cells ... These results suggest that activation of NF-kB can lead to increased expression of bcl-x as manifested by the increase in the short form of bcl-x
Yanagihara et al., J Allergy Clin Immunol 1997 : These results suggest that coordination of NF-kappaB and STAT6 may be required for induction of germline Cepsilon transcription by IL-4, and that CD40 mediated NF-kappaB activation may be important in regulating both enhancement of germline Cepsilon transcription and class switching to IgE
Flaherty et al., J Surg Res 1997 : LPS induced nuclear translocation of nuclear factor kappa B (NF-kappaB) was analyzed by electrophoretic mobility shift assay
Krappmann et al., Immunobiology 1997 : Regulation of NF-kappa B activity by I kappa B alpha and I kappa B beta stability ... Regulation of NF-kappa B activity by I kappa B alpha and I kappa B beta stability ... We found that removal of the TNF-alpha containing medium causes a rapid decrease in nuclear NF-kappa B
Wissink et al., Immunobiology 1997 : NF-kappa B/Rel family members regulating the ICAM-1 promoter in monocytic THP-1 cells
Rager et al., J Virol 1998 (Vaccinia) : Furthermore, dsRNA treatment of Ramos cells resulted in the activation of PKR and in vivo activation of the NF-kappaB complex
Chini et al., J Virol 1998 (Respiratory Syncytial Virus Infections) : Essential roles of NF-kappaB and C/EBP in the regulation of intercellular adhesion molecule-1 after respiratory syncytial virus infection of human respiratory epithelial cell cultures
Pahan et al., J Biol Chem 1998 : Since activation of NF-kappaB is necessary for the induction of iNOS , we examined the effect of SMase and C2-ceramide on the activation of NF-kappaB ... Although SMase or C2-ceramide alone was ineffective in activating NF-kappaB, both stimulated the LPS mediated activation of NF-kappaB in LPS activated astrocytes ... Inhibition of ceramide and LPS mediated induction of iNOS by antioxidant inhibitors of NF-kappaB ( N-acetylcysteine and pyrrolidine dithiocarbamate ) suggest that the stimulatory effect of ceramide on the induction of iNOS is due to the stimulation of NF-kappaB activation and that cellular redox plays a role in the activation of NF-kappaB and induction of iNOS ... Inhibition of LPS mediated as well as LPS and ceramide mediated induction of iNOS and activation of NF-kappaB by PD98059, a specific inhibitor of activation of mitogen activated protein ( MAP ) kinase kinase ( MEK ), and FPT inhibitor II, a selective inhibitor of Ras farnesyl protein transferase, indicate that the Ras-MAP kinase pathway is involved in LPS-ceramide induced activation of NF-kappaB and induction of iNOS, and that ceramide mediated signaling events probably converge into the LPS modulated MAP kinase signaling pathway resulting in greater activation of NF-kappaB and iNOS induction ... Inhibition of LPS mediated as well as LPS and ceramide mediated induction of iNOS and activation of NF-kappaB by PD98059, a specific inhibitor of activation of mitogen activated protein ( MAP ) kinase kinase ( MEK ), and FPT inhibitor II, a selective inhibitor of Ras farnesyl protein transferase, indicate that the Ras-MAP kinase pathway is involved in LPS-ceramide induced activation of NF-kappaB and induction of iNOS, and that ceramide mediated signaling events probably converge into the LPS modulated MAP kinase signaling pathway resulting in greater activation of NF-kappaB and iNOS induction ... This study illustrates a novel role of the sphingomyelin-ceramide signaling pathway in stimulating the expression of iNOS via LPS- or cytokine mediated activation of NF-kappaB in astrocytes ... This study illustrates a novel role of the sphingomyelin-ceramide signaling pathway in stimulating the expression of iNOS via LPS- or cytokine mediated activation of NF-kappaB in astrocytes
Bertrand et al., J Biol Chem 1998 : We now investigated whether the activation of NF-kappaB by insulin could serve an antiapoptotic function ... We also show that insulin antiapoptotic signaling but not insulin activation of NF-kappaB involved phosphatidylinositol 3-kinase ( PI 3-kinase ), as supported by the inhibition of the former but not of the latter process by the PI 3-kinase inhibitor LY294002 ... Insulin antiapoptotic signaling: (i) was dependent on IR number and IR tyrosine kinase activity since it was reduced in parental CHO cells and was abolished in CHO-Y2 cells overexpressing IRs mutated at Tyr1162/1163 ; ( ii ) was, like insulin activation of NF-kappaB , dependent on Raf-1 but not on mitogen activated protein kinase activity since both processes were decreased by the dominant negative Raf-1 mutant Raf-C4 whereas they persisted in mitogen activated protein kinase depleted cells ; and ( iii ) required NF-kappaB activation since it was decreased by proteasome inhibitors and the dominant negative IkappaB-alpha ( A32/36 ) mutant and was mimicked by overexpression of the NF-kappaB c-Rel subunit
Hattori et al., Mol Cell Biochem 1997 : Thus, VCAM-1 gene expression appeared to be mediated by NF-kappa b in cardiac myocytes, and this cardiac myocyte VCAM-1 may be involved in cardiac inflammatory disorders
Bellezzo et al., J Lab Clin Med 1998 (Liver Diseases) : LPS activated NF-kappaB in Kupffer cells, and this response was inhibited by NAC and alpha-TOC ... LPS activated NF-kappaB in Kupffer cells, and this response was inhibited by NAC and alpha-TOC ... LPS activated NF-kappaB in Kupffer cells, and this response was inhibited by NAC and alpha-TOC
Vanden Berghe et al., J Biol Chem 1998 : In the mouse fibrosarcoma cell line L929, the nuclear factor (NF)-kappaB plays a crucial role in IL-6 gene expression mediated by tumor necrosis factor (TNF)
Heese et al., J Neurochem 1998 (Inflammation) : To assess the role of the transcription factor nuclear factor-kappaB (NF-kappaB) in inflammatory mediator induced microglial NGF expression, the effect of the NF-kappaB inhibitor pyrrolidine dithiocarbamate ( PDTC ) was analyzed ... In addition, microglial neurotoxicity mediating beta-amyloid peptides A beta ( 1-40 ) and A beta ( 1-42 ) failed to alter NGF synthesis, whereas A beta ( 25-35 ) specifically induced NF-kappaB dependent microglial NGF expression
Yorek et al., Am J Physiol 1998 : In bovine aorta and bovine pulmonary artery endothelial cells, TNF-alpha activated nuclear factor (NF)-kappa B
Knobil et al., Am J Physiol 1998 (Lung Neoplasms) : Pretreatment of A549 cells with N-acetyl-L-cysteine attenuated virus induced NF-kappa B activation and interleukin (IL)-8 mRNA induction but did not block induction of MnSOD mRNA
Welch et al., Proc Assoc Am Physicians 1998 : Homocysteine induced nitric oxide production in vascular smooth-muscle cells by NF-kappa B-dependent transcriptional activation of Nos2
Brink et al., J Biol Chem 1998 : Tumor necrosis factor receptor (TNFR) associated factor 2A ( TRAF2A ), a TRAF2 splice variant with an extended RING finger domain that inhibits TNFR2 mediated NF-kappaB activation ... Also in contrast to TRAF2, TRAF2A is unable to stimulate NF-kappaB activity when overexpressed in 293 cells and acts as a dominant inhibitor of TNFR2 dependent NF-kappaB activation ... TRAF2A thus represents a novel signal transduction protein, the expression of which can act to inhibit TRAF2 dependent NF-kappaB activation
Jang et al., Biochem Biophys Res Commun 1998 : 4-1BB mediated NF-kappa B activation was inhibited by a dominant negative-TRAF2 or -NF-kappa B-inducing kinase (NIK)
Gerritsen et al., Am J Pathol 1998 : The activation of sphingomyelinase and the generation of ceramide has been proposed to mediate tumor necrosis factor-alpha (TNF-alpha) induced nuclear factor (NF)-kappaB activation through its second messenger ceramide ... TNF-alpha was shown to activate NF-kappaB ( p65 translocation and degradation of IkappaBalpha ) and the stress kinase pathway ( phosphorylation of ATF-2, p38, and c-jun ) ... Thus, although TNF-alpha stimulates the NF-kappaB and stress kinase pathways in HSF, these effects of TNF-alpha are not associated with sphingomyelinase turnover or induction of apoptosis
Kaul et al., Arch Biochem Biophys 1998 : Although PKC activity was modified by H2O2 with a similar time course as H2O2 activation of NF-kappa B , the H2O2 stimulated increase in NF-kappa B DNA binding and translocation to the nucleus was unaffected by PKC inhibitors
Ahmad et al., J Biol Chem 1998 : The inflammatory response transcription factor nuclear factor (NF)-kappaB plays an important role in the regulation of VCAM-1 expression by various stimuli including tumor necrosis factor (TNF)-alpha ... In gel mobility supershift assays, the antibodies to c-Fos or c-Jun inhibited the binding of TNF-alpha activated nuclear NF-kappaB to the kappaL-kappaR, suggesting that both c-Fos and c-Jun interacted with NF-kappaB
Bellocq et al., J Biol Chem 1998 : Low environmental pH-induced iNOS gene transcription involved the activation of nuclear factor-kappaB (NF-kappaB) transcription factor since exposure of macrophages to low environmental pH both increased NF-kappaB binding activity in the nucleus and enhanced NF-kappaB-driven reporter gene expression
Revilla et al., J Biol Chem 1998 : NF-kappaB activation induced by tumor necrosis factor , as detected by mobility shift assays or by transfection of kappaB-driven reporter genes, is impaired in African swine fever virus infected cells
Sakurai et al., Biochem Biophys Res Commun 1998 : TGF-beta activated kinase 1 stimulates NF-kappa B activation by an NF-kappa B-inducing kinase independent mechanism ... A dominant negative mutant of NIK did not inhibit TAK1 induced NF-kappa B activation ... These results suggest that TAK1 induces NF-kappa B activation through a novel NIK independent signaling pathway
Zhu et al., Biochem J 1998 : Cells on fibronectin showed a 1.5-2-fold enhancement in IL-1 induced NF-kappaB activity compared with levels in cells on poly ( l-lysine ) or bare tissue culture plates ... IL-1 induced NF-kappaB activity showed a successive, substratum independent increase during 4 h of attachment and spreading, whereas the inhibitory effect of fibronectin on the SAPK pathway was induced at the initial stages of attachment ... Thus fibronectin attachment causes an up-regulation of NF-kappaB activity in the presence of IL-1 , whereas in contrast it results in a pronounced decrease in IL-1 induced SAPK activity
Pryhuber et al., Am J Physiol 1998 : In the present study, electrophoretic mobility shift assays ( EM-SAs ) and pyrrolidine dithiocarbamate ( PDTC ), an inhibitor of NF-kappa B activation, were utilized to determine the role of NF-kappa B activation in TPA and TNF-alpha inhibition of the surfactant proteins in NCI-H441 cells ... In the present study, electrophoretic mobility shift assays ( EM-SAs ) and pyrrolidine dithiocarbamate ( PDTC ), an inhibitor of NF-kappa B activation, were utilized to determine the role of NF-kappa B activation in TPA and TNF-alpha inhibition of the surfactant proteins in NCI-H441 cells ... By EMSA, TPA and TNF-alpha increased nuclear NF-kappa B binding activity in temporally distinct patterns ... PDTC decreased TPA- and TNF-alpha induced NF-kappa B binding activity but did not limit their inhibition of SP-A and SP-B mRNAs ... The effects of PDTC and PTX on NF-kappa B and the surfactant proteins suggest that NF-kappa B activation does not mediate TPA or TNF-alpha inhibition of SP-A and SP-B mRNA accumulation ... The effects of PDTC and PTX on NF-kappa B and the surfactant proteins suggest that NF-kappa B activation does not mediate TPA or TNF-alpha inhibition of SP-A and SP-B mRNA accumulation
Lockyer et al., Circ Res 1998 : Furthermore, TNF-alpha induced NF-kappaB DNA binding activity was inhibited in the population of endothelial cells expressing IkappaBdeltaN
Wahl et al., J Clin Invest 1998 (Colonic Diseases) : TNFalpha induced nuclear translocation of NF-kappaB was prevented by sulfasalazine through inhibition of IkappaBalpha degradation
Kawamata et al., J Biol Chem 1998 : These results indicate that the NF-kappaB activation resulting from OX40 stimulation is mediated by both TRAF2 and TRAF5, and is likely to be negatively modulated by TRAF3 ... These results indicate that the NF-kappaB activation resulting from OX40 stimulation is mediated by both TRAF2 and TRAF5 , and is likely to be negatively modulated by TRAF3
Carter et al., Am J Respir Cell Mol Biol 1998 (Respiratory Distress Syndrome, Adult) : Lipopolysaccharide induced NF-kappaB activation and cytokine release in human alveolar macrophages is PKC independent and TK- and PC-PLC dependent ... In these studies, we evaluated the regulation of LPS induced cytokine release and the activation of NF-kappaB in human AM ... We also found that LPS induced activation of NF-kappaB was enhanced in AM cultured in serum or in the presence of LPS binding protein, simulating conditions in the lung that are present in ARDS ... In addition, LPS triggered the activation of several different NF-kappaB complexes in AM, and different forms of NF-kappaB bound to the IL-6, IL-8, and TNF promoter sequences
Mantovani et al., Nucleic Acids Res 1998 : NF-kappaB mediated transcriptional activation is enhanced by the architectural factor HMGI-C
Kang et al., J Biol Chem 1998 : Moreover, the activation of nuclear factor kappaB (NFkappaB) induced by extracellularly added H2O2 or tumor necrosis factor-alpha was blocked by overproduction of Prx II
Gri et al., J Biol Chem 1998 (Cell Transformation, Viral) : Synergistic regulation of the human interleukin-12 p40 promoter by NFkappaB and Ets transcription factors in Epstein-Barr virus transformed B cells and macrophages ... Synergistic regulation of the human interleukin-12 p40 promoter by NFkappaB and Ets transcription factors in Epstein-Barr virus transformed B cells and macrophages ... While the NFkappaB and Ets sites appear equally critical for inducible p40 promoter activity in macrophage cell lines, NFkappaB plays a more dominant role in the constitutive p40 promoter activity in EBV+ B cells
Ravi et al., Cancer Res 1998 : We demonstrate that ligation of CD95 ( Fas/APO1 ), a potent apoptotic stimulus in lymphocytes, results in repression of NF-kappaB activity in Jurkat T cells by inducing the proteolytic cleavage of NF-kappaB p65 ( Rel A ) and p50
Relaix et al., Nat Genet 1998 : TNF signal transduction is mediated by TRAF2 which binds the TNF Receptor2 (TNFR2) and activates NFkappaB ... Peg3 expression activates NFkappaB via IkappaB-NFkappaB dissociation and acts synergistically with TRAF2 ... Transfection of a truncated Peg3 containing the TRAF2 interaction site, abolishes NFkappaB activation by TRAF2 and/or TNF ... Transfection of a truncated Peg3 containing the TRAF2 interaction site, abolishes NFkappaB activation by TRAF2 and/or TNF
Bearz et al., Biochem Biophys Res Commun 1998 : Fibronectin binding promotes a PKC dependent modulation of NF-kappa B in human T cells ... The upregulation of NF-kappa B activity was abrogated by calphostin C, an inhibitor of protein kinase C. Cell adhesion determined a strong reduction in the cytoplasmic levels of the NF-kappa B inhibitor I kappa B alpha, reduction that was prevented after treatment with calphostin C, suggesting that PKC dependent I kappa B alpha phosphorylation might be involved in the upregulation of NF-kappa B
Stehlik et al., Biochem Biophys Res Commun 1998 : We demonstrate that adenovirus mediated overexpression of I kappa B alpha , an inhibitor of NF-kappa B suppresses the expression of piap in response to TNF-alpha suggesting that piap is one of the NF-kappa B regulated genes that operates to prevent programmed cell death of EC in inflammation
Miyazawa et al., Am J Pathol 1998 (Arthritis, Rheumatoid...) : Constitutive transcription of the human interleukin-6 gene by rheumatoid synoviocytes : spontaneous activation of NF-kappaB and CBF1 ... Electrophoretic mobility shift assays revealed that the binding activity of p50 and p65 NF-kappaB subunits and CBF1 was significantly enhanced in the IL-6 high producing clones compared with that of IL-6 low producing clones and cultured sarcoma cells, suggesting that spontaneous activation of NF-kappaB and CBF1 may lead to the constitutive transcription of the IL-6 gene by rheumatoid FLSs
Mukherjee et al., J Interferon Cytokine Res 1998 (Leukemia, Monocytic, Acute) : TNF dependent activation of NF-kappaB could be blocked partially by both anti-p60 and anti-p80 , suggesting that TNF mediates its effect independently through the p60 and p80 receptors ... TNF dependent activation of NF-kappaB could be blocked partially by both anti-p60 and anti-p80, suggesting that TNF mediates its effect independently through the p60 and p80 receptors ... TNF dependent activation of NF-kappaB could be blocked partially by both anti-p60 and anti-p80, suggesting that TNF mediates its effect independently through the p60 and p80 receptors ... In comparison, the activation of NF-kappaB by lymphotoxin ( LT ), which shares receptors with TNF, was completely blocked by anti-p60 , whereas anti-p80 had no effect
Bergmann et al., J Biol Chem 1998 : Two closely related IkappaBalpha kinases as well as the upstream kinase, NIK, which integrates interleukin-1beta (IL-1beta)- and tumor necrosis factor (TNF)-alpha dependent activation of the transcription factor NF-kappaB have recently been described ... Two closely related IkappaBalpha kinases as well as the upstream kinase, NIK, which integrates interleukin-1beta (IL-1beta)- and tumor necrosis factor (TNF)-alpha dependent activation of the transcription factor NF-kappaB have recently been described ... However, IL-1beta induced IkappaBalpha degradation as well as NF-kappaB nuclear translocation and DNA binding, as determined by Western blot and electro-mobility shift assay, respectively, are not affected by these inhibitors ... However, IL-1beta induced IkappaBalpha degradation as well as NF-kappaB nuclear translocation and DNA binding, as determined by Western blot and electro-mobility shift assay, respectively, are not affected by these inhibitors
Pan et al., J Immunol 1998 : The role of NF-kappaB activation in BK-induced IL-1beta synthesis was demonstrated by the ability of BK to stimulate increased chloramphenicol acetyltransferase (CAT) activity in A549 cells transfected with a reporter plasmid containing three kappaB enhancers from the IL-1beta gene, while deletion of the kappaB enhancer sequences eliminated BK-stimulated CAT activity ... C3 transferase exoenzyme, an inhibitor of Rho, abolished BK-induced NF-kappaB activation at 10 microg/ml and significantly inhibited BK-stimulated IL-1beta synthesis at 5 microg/ml
Inoue et al., Jpn J Cancer Res 1998 (Leukemia, T-Cell) : These findings in vivo are strikingly different from those in HTLV-1 infected T cell lines in vitro, in which Tax is responsible for NF-kappaB activation
Chen et al., Ann Clin Lab Sci 1998 : Catalase , metal chelator, deferoxamine, and the silanol group ( SiOH ) blocker, poly ( 2-vinylpyridine-N-oxide ) ( PVPNO ), also inhibited silica induced NF-kappa B activation ... The antioxidant, ascorbate, attenuated the NF-kappa B activation induced by silica but not by LPS ... The .OH radical scavenger, sodium formate, inhibited NF-kappa B activation induced by silica but had only a minor effect on NF-kappa B activation induced by LPS
Wissink et al., Mol Endocrinol 1998 : However, this enhanced IkappaBalpha synthesis did not cause repression of NF-kappaB DNA binding activity
Scheuren et al., Br J Pharmacol 1998 : The effect of ibuprofen was specific because, at concentrations up to 10 mM, ibuprofen did not affect the heat shock transcription factor ( HSF ) and the activation of NF-kappaB by prostaglandin E2 ( PGE2 )
Nakano et al., Proc Natl Acad Sci U S A 1998 : A previously identified NF-kappaB inducing kinase (NIK), which mediates NF-kappaB activation by TNFalpha and IL-1, has been demonstrated to activate IKKalpha ... A previously identified NF-kappaB inducing kinase (NIK), which mediates NF-kappaB activation by TNFalpha and IL-1 , has been demonstrated to activate IKKalpha ... Moreover, a catalytically inactive mutant of IKKbeta blocked the MEKK1 induced NF-kappaB activation
Yoshizaki et al., Proc Natl Acad Sci U S A 1998 (Cell Transformation, Neoplastic...) : Here we report that binding sites for NF-kappaB , SP-1, and AP-1 also contribute to induction of the MMP9 promoter by the viral protein, LMP1, mainly through the NF-kappaB and, to a lesser extent, the SP-1 and AP-1 sites
Ling et al., Proc Natl Acad Sci U S A 1998 : A mutant form of IKK-alpha containing alanine at residue 176 can not be phosphorylated or activated by NIK and acts as a dominant negative inhibitor of interleukin 1- and tumor necrosis factor induced NF-kappaB activation ... Thus, the phosphorylation of IKK-alpha on Ser-176 by NIK may be required for cytokine mediated NF-kappaB activation
Natarajan et al., Arch Biochem Biophys 1998 : The NF-kappaB dependent CAT reporter gene expression in transient transfection assays was also suppressed by the PTK inhibitors
Lieb et al., J Neurochem 1998 (Astrocytoma) : Promoter deletion analysis revealed that IL-1beta induced IL-6 expression required the transcription factor nuclear factor-kappaB (NF-kappaB) , whereas SP- and histamine induced IL-6 synthesis was essentially controlled by NF-IL-6
Moffatt et al., J Virol 1998 : Furthermore, using pentoxifylline, an inhibitor of NF-kappaB activation, we demonstrate that the NF-kappaB mediated IL-6 activation by NS1 is uncoupled from the apoptotic pathway ... Furthermore, using pentoxifylline, an inhibitor of NF-kappaB activation, we demonstrate that the NF-kappaB mediated IL-6 activation by NS1 is uncoupled from the apoptotic pathway
Bauer et al., J Biol Chem 1998 : Moreover, inhibition of NF-kappaB activity by a transdominant IkappaB mutant attenuated CD95L expression
Zechner et al., J Biol Chem 1998 : Interestingly, overexpression of a mutant form of I-kappaBalpha , which inhibits nuclear translocation of NF-kappaB, completely blocked MKK6 ( Glu ) -activated NF-kappaB but had little effect on MKK6s anti-apoptotic effects ... Additionally, while NF-kappaB is activated in myocardial cells by p38 , this does not appear to be the major mechanism by which MKK6 ( Glu ) exerts its anti-apoptotic effects in this cell type, suggesting a novel pathway for p38 mediated protection from apoptosis
Oswald et al., Mol Cell Biol 1998 : By performing transient-transfection assays, we show that human RBP-Jkappa represses basal as well as RelA ( p65 ) -stimulated NF-kappaB2 promoter activity ... In transient-transfection assays we show that truncated Notch-1 strongly induces NF-kappaB2 promoter activity
Bex et al., Mol Cell Biol 1998 (Cell Transformation, Viral) : These studies are consistent with a model in which activation of either the NF-kappaB or the ATF/CREB pathway by specific Tax mutants is mediated by distinct interactions with related coactivator proteins
Hellin et al., Oncogene 1998 (Colonic Neoplasms) : Under our conditions, the free radical scavengers NAC and PDTC were not able to block NF-kappaB activation or p53 induction , indicating that reactive oxygen intermediates were not involved in the cellular response to daunomycin stimulation ... Overexpression of a stable unresponsive IkappaBalpha mutant in HCT116 cells resulted in a complete inhibition of the NF-kappaB activation but only a partial impairment of the p53 protein accumulation induced by daunomycin
Kikumori et al., Endocrinology 1998 : The present study examined the effects of TNF-alpha on the activation of nuclear factor-kappa B (NF-kappaB) and on the expression of interleukin (IL)-6 gene in rat thyroid FRTL-5 cells ... These results strongly suggest that TNF-alpha increases the IL-6 gene expression through the activation of NF-kappaB in the thyroid cells, and that antioxidants suppress the TNF-alpha dependent IL-6 expression by inhibiting the activation of the transcriptionally active NF-kappaB
de Wit et al., Leukemia 1998 (Inflammation) : In transient transfection assays we demonstrated that p100 has an inhibitory effect on the NF-kappaB dependent IL-6 promoter activity ... We therefore examined the composition of NF-kappaB induced by LPS vs the different lymphokines ... LPS induced NF-kappaB showed a distinct p65 supershift whereas the composition of NF-kappaB induced by different lymphokines did not show a change in p65
Kelliher et al., Immunity 1998 (Failure to Thrive) : The death domain kinase RIP mediates the TNF induced NF-kappaB signal
Ishikawa et al., J Exp Med 1998 (Inflammation...) : Loss of p105 also led to enhanced constitutive p50 homodimer and inducible NF-kappaB activities in unstimulated and stimulated cells, respectively
Horie et al., Int Immunol 1998 : Activation of NFkappaB by CD30 was shown to be mediated through interaction of TNFR associated factor (TRAF) 1, 2 and 5 with the D2 and D3 subdomains ... Electrophoretic mobility shift assays revealed constitutive and CD30 induced NFkappaB activation in stable transformants of 293 cells expressing CD30 or a deletion mutant lacking D2 and D3 subdomains
Griswold et al., Inflamm Res 1998 (Dermatitis, Contact...) : The thickness and influx of neutrophils into the treated ears was measured as was the effects of the azaspiranes on 5-lipoxygenase activity, cyclooxygenase activity, prostaglandin and leukotriene synthesis, and the activation of the transcription factor NF-kappaB
Ng et al., Biochem Biophys Res Commun 1998 : In addition, the x1c domain is also crucial for TNFR2 mediated NF-kappa B activation
Jimi et al., J Biol Chem 1998 : An electrophoretic mobility shift assay showed that IL-1 transiently activated NF-kappaB in the nuclei of the OCLs, and the maximal activation occurred at 30 min ... The pretreatment of purified OCLs with proteasome inhibitors suppressed the IL-1 induced activation of NF-kappaB and prevented the survival of OCLs supported by IL-1
Moitreyee et al., Mol Cell Biochem 1998 (Leukemia, Erythroblastic, Acute) : Potential role of NF-kB and RXR beta like proteins in interferon induced HLA class I and beta globin gene transcription in K562 erythroleukaemia cells
Bonizzi et al., J Immunol 1997 : Indeed, inhibition of receptor internalization as well as NH4Cl and chloroquine blocked IL-1beta mediated induction of NF-kappaB in OVCAR-3 and in other epithelial cell lines but not in lymphoid cells, indicating that distinct pathways are involved ... Moreover, expression of the 5-lipoxygenase (5-LOX) enzyme was not detected in epithelial cells, and inhibition of this enzyme blocked NF-kappaB induction by IL-1beta only in lymphoid cells ... This study thus indicates that the activation of NF-kappaB following IL-1beta treatment involves the activation of phospholipase A2 and 5-LOX and the production of reactive oxygen intermediates ( ROIs ) in lymphoid cells, while in epithelial cells, another pathway predominates and could involve the acid sphingomyelinase
Parry et al., J Immunol 1997 : These results suggest that activation of the protein kinase A pathway inhibits NF-kappaB transcription by phosphorylating CREB , which competes with p65 for limiting amounts of CBP
Weng et al., J Immunol 1997 : To further explore the ramifications of CD19 signaling, the current study examined whether phosphorylation of Elk-1, activation of activator protein-1 (AP-1) , or activation of nuclear factor-kappaB (NF-kappaB) transcription factors occurred following CD19 cross linking ... In contrast, CD19 cross linking in BLIN-1 cells resulted in the induction of NF-kappaB , but had no apparent effect on AP-1 binding activity
Anrather et al., J Immunol 1997 : Similar potentiation of TNF induced NF-kappaB activity and E-selectin transcription by thrombin was observed in experiments utilizing luciferase reporter constructs expressed in bovine aortic EC
Trepicchio et al., J Immunol 1997 (Inflammation) : Analysis of rhIL-11 effects on transcription factors that activate proinflammatory cytokines demonstrated that the level of LPS induced NF-kappaB binding activity in the nucleus of rhIL-11 treated peritoneal macrophages was significantly reduced
Zhu et al., J Virol 1998 : Furthermore, we observed that the core protein blocked the TNF induced activation of RelA/NF-kappaB in murine BC10ME cells, thus at least partially accounting for the increased sensitivity of BC10ME cells to TNF
Johnson et al., J Virol 1998 : Activation of NF-kappaB by CAO-LMP1 was not impaired in any of the lines ; rather, activation of an NF-kappaB reporter by CAO-LMP1 was consistently about twofold greater than the activation with B95.8- or AG876-LMP1
Ochoa-Garay et al., J Immunol 1998 : Expression of a degradation-resistant form of IkappaBalpha in DPK cells results in constitutive inhibition of NF-kappaB activity
Bushdid et al., Nature 1998 : Furthermore, inhibition of NF-kappaB activity in limb mesenchyme leads to a reduction in expression of Sonic hedgehog and Twist but derepresses expression of the bone morphogenetic protein-4 gene
Marsters et al., Curr Biol 1998 : Dominant negative mutants of several factors that play a key role in NF-kappaB induction by TNF [ 10 ] inhibited NF-kappaB activation by Apo3L
Palmetshofer et al., Transplantation 1998 : BS-I strongly induced E-selectin , P-selectin, intercellular adhesion molecule-1, and interleukin-8 mRNA in an NF-kappaB dependent manner ... BS-I strongly induced E-selectin, P-selectin , intercellular adhesion molecule-1, and interleukin-8 mRNA in an NF-kappaB dependent manner ... BS-I strongly induced E-selectin, P-selectin, intercellular adhesion molecule-1 , and interleukin-8 mRNA in an NF-kappaB dependent manner
Baumann et al., J Biol Chem 1998 (Plasmacytoma) : Interestingly, both the potent physiological inducer of NF-kappaB TNFalpha as well as endoplasmic reticulum overload can induce NF-kappaB via a PDTC sensitive pathway
Miller et al., Mol Cell Biol 1998 : The Epstein-Barr virus latent membrane protein 1 (LMP1) oncoprotein causes multiple cellular changes, including induction of epidermal growth factor receptor (EGFR) expression and activation of the NF-kappaB transcription factor ... Overexpression of TRAF2 potently activates NF-kappaB , although TRAF2 did not induce expression of the EGFR either alone or in combination with TRAF1 and TRAF3
Kwon et al., Diabetes 1998 : TLCK and MG 132 inhibited both IL-1beta induced activation of NFkappaB and degradation of IkappaBalpha by islets and RINm5F cells
Oyama et al., J Immunol 1998 : VEGF significantly inhibited nuclear factor-kappa B (NF-kappa B)-dependent activation of reporter gene transcription during the first 24 h in culture
Lahdenpohja et al., J Immunol 1998 : Simultaneously applied H2O2 strongly potentiated the PDBu- or TNF induced transcriptional activity of NF-kappa B
Roy et al., Biochem Biophys Res Commun 1998 : Interestingly, morphine treatment also modulated the activation of NF kappa B by LPS
Dehbi et al., Oncogene 1998 : Activation of the wt1 Wilms ' tumor suppressor gene by NF-kappaB
Sun et al., Oncogene 1998 : EGF activates NF-kappaB in A-431 carcinoma cells, which overexpress EGF receptors and in mouse embryo fibroblasts, which have a normal complement of receptors ... Supershift experiments indicate that the NF-kappa-B complexes induced by EGF are composed of p50/p50 homodimers and p65/p50 heterodimers, but not c-rel. EGF stimulation enhances the degradation of IkappaBalpha, but not IkappaBbeta nor an N-terminal deletion mutant of IkappaBalpha ... Treatment of cells with a proteasome inhibitor, such as ALLN or MG132, blocks EGF mediated NF-kappaB activation, indicating that EGF induced NF-kappa-B activation requires proteasome dependent IkappaB degradation ... Also, Bapta A/M ( a cell-permeable chelator of intracellular calcium ) blocks EGF induced NF-kappa-B activation and IkappaBalpha degradation, suggesting a requirement of intracellular free Ca2+ for this growth factor response ... Protein kinase C inhibition, in contrast, did not influence EGF activation of NF-kappaB
Wood et al., Oncogene 1998 (Hodgkin Disease) : Thus the constitutive expression of NF-kappaB in Hodgkin cells is a direct consequence of the abnormal expression of IkappaBalpha rather than changes in NF-kappaB that render it refractory to inhibition by IkappaB proteins
Medvedev et al., J Immunol 1998 : This study was undertaken to evaluate the role of CD14 and complement receptors type 3 ( CR3 ) and 4 ( CR4 ) in mediating TNF release and NF-kappaB activation induced by LPS and cell wall preparations from group B streptococci type III ( GBS ) ... This study was undertaken to evaluate the role of CD14 and complement receptors type 3 ( CR3 ) and 4 ( CR4 ) in mediating TNF release and NF-kappaB activation induced by LPS and cell wall preparations from group B streptococci type III ( GBS ) ... LPS also activated NF-kappaB in CHO cells transfected with either human CR3 or CR4 cDNA, although responses were delayed and weaker than those of CHO/CD14 cells
Burns et al., J Biol Chem 1998 : Overexpression of MyD88 induces activation of the c-Jun N-terminal kinase (JNK) and the transcription factor NF-kappaB through its DD ... MyD88 induced NF-kappaB activation is inhibited by the dominant negative versions of TRAF6 and IRAK, which also inhibit IL-1 induced NF-kappaB activation ... MyD88 induced NF-kappaB activation is inhibited by the dominant negative versions of TRAF6 and IRAK, which also inhibit IL-1 induced NF-kappaB activation ... Overexpression of MyD88-lpr or MyD88-Toll ( expressing only the Toll domain ) acted to inhibit IL-1 induced NF-kappaB and JNK activation in a 293 cell line overexpressing the IL-1RI ... Overexpression of MyD88-lpr or MyD88-Toll ( expressing only the Toll domain ) acted to inhibit IL-1 induced NF-kappaB and JNK activation in a 293 cell line overexpressing the IL-1RI
Pahan et al., J Biol Chem 1998 : Because the activation of NF-kappaB is necessary for the induction of iNOS and the expression of tumor necrosis factor (TNF)-alpha also depends on the activation of NF-kappaB, we examined the effect of okadaic acid on the LPS mediated activation of NF-kappaB and production of TNF-alpha in rat primary astrocytes and macrophages ... Because the activation of NF-kappaB is necessary for the induction of iNOS and the expression of tumor necrosis factor (TNF)-alpha also depends on the activation of NF-kappaB, we examined the effect of okadaic acid on the LPS mediated activation of NF-kappaB and production of TNF-alpha in rat primary astrocytes and macrophages ... Interestingly, in both cell types, okadaic acid stimulated the LPS mediated DNA binding as well as transcriptional activity of NF-kappaB and production of TNF-alpha ... This study suggests that the stimulation of iNOS expression in astrocytes by inhibitors of PP 1/2A is possibly due to the stimulation of NF-kappaB activation ; however, activation of NF-kappaB is not sufficient for the induction of iNOS in macrophages and that apart from NF-kappaB some other signaling pathway ( s ) sensitive to PP 1 and/or PP 2A is/are possibly involved in the regulation of iNOS in macrophages ... This differential induction of iNOS as compared with similar activation of NF-kappaB by inhibitors of PP 1/2A indicates the involvement of different intracellular signaling events for the induction of iNOS in two cell types of the same animal species ... This differential induction of iNOS as compared with similar activation of NF-kappaB by inhibitors of PP 1/2A indicates the involvement of different intracellular signaling events for the induction of iNOS in two cell types of the same animal species
Francis et al., Int Immunol 1998 : However, with continued stimulation RelB completely disappeared from anti-Ig stimulated kappaB binding material, but remained a component of CD40L induced NF-kappaB
Yang et al., Proc Natl Acad Sci U S A 1998 : A human cell line that is resistant to the antiviral and antiproliferative activities of IFN but is still IFN-responsive by virtue of STAT1 and STAT2 activation was found to be defective in STAT3 activation and in induction of NF-kappaB DNA binding activity ... Because STAT3 is involved in the induction of NF-kappaB DNA binding activity and in the induction of antiviral and antiproliferative activity, our results place STAT3 as an important upstream element in type I IFN signal transduction and in the induction of biological activities
Li et al., Proc Natl Acad Sci U S A 1998 (Cystic Fibrosis...) : Activated NF-kappaB binds to a kappaB site in the 5'-flanking region of the MUC2 gene and activates MUC2 mucin transcription ... Activated NF-kappaB binds to a kappaB site in the 5'-flanking region of the MUC2 gene and activates MUC2 mucin transcription
Iuvone et al., Br J Pharmacol 1998 : Recent studies have shown that the expression of iNOS is regulated at the transcriptional level by a transcription nuclear factor-kappaB (NF-kappaB) ... Moreover, PDTC ( 0.1, 1 micromol/site ) suppressed LPS induced NF-kappaB DNA binding
Mahboubi et al., J Pharmacol Exp Ther 1998 : Because the PGHS-2 promoter has a nuclear factor-kappa B (NF-kappa B) binding motif, which is important for PGHS-2 gene transcription in some cell types, we have evaluated the effects of tyrosine kinase inhibitors and PAO on TNF-alpha induced NF-kappa B activation ... N-tosyl-L-phenylalanine chloromethyl ketone ( TPCK ), a serine protease inhibitor, inhibited I kappa B-alpha degradation and NF-kappa B activation in response to TNF-alpha in a dose dependent manner ( 25, 50, 100 microM ) ... These data suggest that NF-kappa B contributed to PGHS-2 mRNA accumulation in MCA-101 cells stimulated with TNF-alpha ... These data demonstrate that tyrosine kinase pathways are not required for TNF-alpha induced NF-kappa B activation in MCA-101 cells and suggest that signaling via these pathways mediates TNF-alpha induced PGHS-2 mRNA accumulation via an NF-kappa B-independent mechanism
Casado et al., Biochem J 1997 : Because iNOS transcription is highly dependent on the activation of nuclear factor kappaB (NF-kappaB) , this factor was evaluated by electrophoretic mobility shift assays, and a close correlation between NF-kappaB activity and iNOS mRNA levels was observed
Hellerbrand et al., Hepatology 1998 (Liver Cirrhosis, Experimental) : Cytokines induce NFkappaB activity in activated but not in quiescent HSCs with subsequent expression of NFkappaB-responsive genes, such as intercellular adhesion molecule (ICAM)-1 and interleukin (IL)-6 ... Cytokines induce NFkappaB activity in activated but not in quiescent HSCs with subsequent expression of NFkappaB-responsive genes, such as intercellular adhesion molecule (ICAM)-1 and interleukin (IL)-6 ... Proteasome inhibitors, which block the degradation of IkappaB, and the Ad5IkappaB , which provides an exogenous nondegradable IkappaB, block the stimulation of NFkappaB activity by TNFalpha and IL-1beta in activated HSCs ... Proteasome inhibitors, which block the degradation of IkappaB, and the Ad5IkappaB, which provides an exogenous nondegradable IkappaB, block the stimulation of NFkappaB activity by TNFalpha and IL-1beta in activated HSCs ... Proteasome inhibitors, which block the degradation of IkappaB, and the Ad5IkappaB, which provides an exogenous nondegradable IkappaB, block the stimulation of NFkappaB activity by TNFalpha and IL-1beta in activated HSCs
DeLuca et al., Virology 1998 : Inhibition of TNFalpha induced NF-kappaB activation using the antioxidant N-acetylcysteine (NAC) resulted in increased apoptosis in both U937 and U9-IIIB cells, while preactivation of NF-kappaB with the non-apoptotic inducer IL-1beta caused a relative decrease in apoptosis ... The protective role of NF-kappaB in blocking TNFalpha- and HIV-1 induced apoptosis was supported by studies in Jurkat T cells engineered to express IkappaB alpha repressor mutants ( TD-IkappaB ) under the control of a tetracycline-responsive promoter
Montaner et al., J Biol Chem 1998 : Activated forms of Dbl, Ost , and Vav proteins induce NF-kappaB activation ... Finally, mutants of RhoA and Cdc42Hs , but not that of Rac1, inhibited the activation of NF-kappaB by Ost ... Finally, mutants of RhoA and Cdc42Hs, but not that of Rac1, inhibited the activation of NF-kappaB by Ost ... Finally, mutants of RhoA and Cdc42Hs, but not that of Rac1, inhibited the activation of NF-kappaB by Ost ... However, there is a link between the NF-kappaB and the c-Jun N-terminal kinase/stress activated protein kinase ( JNK/SAPK ) cascades since a dominant negative mutant of MEKK1 is able to inhibit NF-kappaB activation induced by Rac1 and Cdc42Hs proteins, but not by RhoA
Manna et al., J Biol Chem 1998 : Overexpression of manganese superoxide dismutase ( Mn-SOD ) in human breast cancer MCF-7 cells completely abolished TNF mediated NF-kappaB activation, IkappaB alpha degradation, p65 nuclear translocation, and NF-kappaB dependent reporter gene expression ... Besides TNF, phorbol ester-, okadaic acid-, ceramide-, and lipopolysaccharide induced activation of NF-kappaB was blocked by Mn-SOD , indicating a common pathway of activation
Akiba et al., J Biol Chem 1998 : CD27 , a member of the tumor necrosis factor receptor superfamily, activates NF-kappaB and stress activated protein kinase/c-Jun N-terminal kinase via TRAF2, TRAF5, and NF-kappaB inducing kinase ... Here we demonstrate that overexpression of CD27 activates NF-kappaB and stress activated protein kinase ( SAPK ) /c-Jun N-terminal kinase (JNK) ... Co-transfection of a dominant negative TRAF2 or TRAF5 blocked NF-kappaB and SAPK/JNK activation induced by CD27 ... These results indicate that TRAF2 and TRAF5 are involved in NF-kappaB and SAPK/JNK activation by CD27, and NIK is a common downstream kinase of TRAF2 and TRAF5 for NF-kappaB and SAPK/JNK activation ... These results indicate that TRAF2 and TRAF5 are involved in NF-kappaB and SAPK/JNK activation by CD27, and NIK is a common downstream kinase of TRAF2 and TRAF5 for NF-kappaB and SAPK/JNK activation
Miller et al., Oncogene 1998 (Nasopharyngeal Neoplasms) : In this study, the LMP1 proteins derived from the laboratory strain B95-8 and the NPC strain C15 were analysed for their ability to activate NF-kappa B and also to induce expression of the EGFR ... The data suggest that the C15-LMP1 protein activates NF-kappa B more efficiently and induces higher levels of the EGFR ... Finally, these data provide evidence that five amino acid changes within the transmembrane domain in the C15-LMP1 protein lead to enhanced NF-kappa B activation and EGFR induction
Kalli et al., Mol Cell Biol 1998 : IL-1 alone stimulates an acute but transient NF-kappaB nuclear localization and a suboptimal NF-kappaB transcriptional response ... Together, these results suggest a mechanism by which signals from the T-cell antigen receptor and either CD28 or IL-1 synergistically regulate IL-2 gene transcription by modulating NF-kappaB nuclear translocation ... Together, these results suggest a mechanism by which signals from the T-cell antigen receptor and either CD28 or IL-1 synergistically regulate IL-2 gene transcription by modulating NF-kappaB nuclear translocation ... Together, these results suggest a mechanism by which signals from the T-cell antigen receptor and either CD28 or IL-1 synergistically regulate IL-2 gene transcription by modulating NF-kappaB nuclear translocation
Guermah et al., Mol Cell Biol 1998 : Transcriptional activation by NF-kappaB and Sp1 requires both TFIID and the USA fraction
Scatena et al., J Cell Biol 1998 : The alphavbeta3 integrin was most important for osteopontin mediated NF-kappaB induction and survival, since adding a neutralizing anti-beta3 integrin antibody blocked NF-kappaB activity and induced endothelial cell death when cells were plated on osteopontin ... Activation of NF-kappaB by osteopontin depended on the small GTP binding protein Ras and the tyrosine kinase Src, since NF-kappaB reporter activity was inhibited by Ras and Src dominant negative mutants ... Activation of NF-kappaB by osteopontin depended on the small GTP binding protein Ras and the tyrosine kinase Src, since NF-kappaB reporter activity was inhibited by Ras and Src dominant negative mutants ... In contrast, inhibition of MEK and PI3-kinase did not affect osteopontin induced NF-kappaB activation ... In contrast, inhibition of MEK and PI3-kinase did not affect osteopontin induced NF-kappaB activation
Kumar et al., Biochem Pharmacol 1998 : As the induction of various adhesion molecules by TNF requires activation of the transcription factor NF-kappaB , the effect of curcumin on the activation of this factor in the EC was also investigated
Lentsch et al., Am J Pathol 1998 (Alveolitis, Extrinsic Allergic...) : NF-kappaB activation during IgG immune complex induced lung injury : requirements for TNF-alpha and IL-1beta but not complement ... NF-kappaB activation during IgG immune complex induced lung injury : requirements for TNF-alpha and IL-1beta but not complement ... In the current studies we sought to determine whether TNF-alpha , IL-1beta, the complement system and oxidants contribute to the activation of NF-kappaB in the lung ... In the current studies we sought to determine whether TNF-alpha, IL-1beta , the complement system and oxidants contribute to the activation of NF-kappaB in the lung ... In the current studies we sought to determine whether TNF-alpha, IL-1beta , the complement system and oxidants contribute to the activation of NF-kappaB in the lung ... N-acetylcysteine, but not catalase , suppressed activation of lung NF-kappaB
Giri et al., J Biol Chem 1998 (Lymphoma, T-Cell...) : TNF activated NF-kappaB in Jurkat cells but not in HuT-78 cells ... HuT-78 cells were also resistant to NF-kappaB activation induced by phorbol ester, H2O2, ceramide, endotoxin, and interleukin-1
Jin et al., Infect Immun 1998 (Lung Diseases...) : Mouse secretory leukocyte protease inhibitor ( SLPI ) was recently characterized as a lipopolysaccharide (LPS) induced product of macrophages that antagonizes their LPS induced activation of NF-kappaB and production of NO and tumor necrosis factor (TNF) ( F. Y. Jin, C. Nathan, D. Radzioch, and A. Ding, Cell 88 : 417-426, 1997 )
Nilakantan et al., Carcinogenesis 1998 : Liver-specific catalase expression in transgenic mice inhibits NF-kappaB activation and DNA synthesis induced by the peroxisome proliferator ciprofibrate ... Ciprofibrate increased the activation of nuclear factor (NF)-kappaB in non-transgenic mice, but this increase was inhibited by catalase overexpression
Washizu et al., Immunogenetics 1998 : Taken together, the binding of nuclear protein to the NF-kappaB binding site is required for transcriptional activation of the IL-15 gene in LPS stimulated macrophages
Clarke et al., Eur J Immunol 1998 : IL-10 mediated suppression of TNF-alpha production is independent of its ability to inhibit NF kappa B activity ... In this study we observed that IL-10 inhibited nuclear NF kappa B DNA binding activity without affecting I kappa B degradation or translocation of NF kappa B subunits to the nucleus ... Therefore we conclude that although IL-10 or IL-4 can suppress NF kappa B activity, this appears to have little effect on the expression of the TNF-alpha gene and is unlikely to be the basis of the anti-inflammatory effects of these cytokines ... Therefore we conclude that although IL-10 or IL-4 can suppress NF kappa B activity, this appears to have little effect on the expression of the TNF-alpha gene and is unlikely to be the basis of the anti-inflammatory effects of these cytokines
Ohtani et al., J Biol Chem 1998 : Both NF-kappaB-like elements were able to bind to NF-kappaB or its related factor ( s ) in a Tax dependent manner
Sugita et al., Inflammation 1998 : These results indicate that P. gingivalis LPS activates NF-kappa B and AP-1 in both serum dependent and -independent manners, followed by increased IL-8 transcription in neutrophils, and suggested a role for P. gingivalis LPS in IL-8 synthesis by neutrophils in inflamed gingiva and GCF
Aoki et al., Am J Physiol 1998 : There was increased IL-8 nuclear factor-kappaB (NF-kappaB) DNA binding activity after histamine stimulation, and this was inhibited by DPH and MK-886
Jung et al., Radiat Res 1998 (Ataxia Telangiectasia) : We report here that expression of a dominant negative form of IkappaB-alpha , an inhibitor of NF-kappaB , protects AT fibroblasts from apoptosis induced by gamma radiation, but it enhances apoptosis in normal fibroblasts ... These data suggest that regulation of NF-kappaB may play an important role in programmed cell death induced by DNA damage in AT cells
Taylor et al., J Biol Chem 1998 : The addition of NF-kappaB inhibitors significantly suppressed cytokine stimulated iNOS mRNA expression and NO synthesis, indicating that NF-kappaB is involved in the induction of the human iNOS gene ... These data indicate that NF-kappaB activation is required for cytokine induction of the human iNOS gene and identifies four NF-kappaB enhancer elements upstream in the human iNOS promoter that confer inducibility to tumor necrosis factor-alpha and interleukin-1beta
Heitmeier et al., J Biol Chem 1998 : Double stranded RNA induced inducible nitric-oxide synthase expression and interleukin-1 release by murine macrophages requires NF-kappaB activation ... Double stranded RNA induced inducible nitric-oxide synthase expression and interleukin-1 release by murine macrophages requires NF-kappaB activation
Jeremias et al., Blood 1998 : We demonstrate here that TRAIL ( TNF related apoptosis inducing ligand ), a recently identified DIL, also activates NFkappaB in lymphoid cell lines in a kinetic similar to TNFalpha
Arima et al., Leuk Res 1998 (Leukemia, Prolymphocytic...) : The binding of interleukin-2 (IL-2) to its receptor on normal T cells induces nuclear expression of nuclear factor kappaB (NF-kappaB) , activation of the IL-2 receptor (IL-2R) alpha chain gene, and cell proliferation ... Electrophoretic mobility-shift assays revealed that recombinant IL-2 increased NF-kappaB binding activity in nuclear extracts of the leukemia cells, and Northern blot analysis showed that IL-2 increased the abundance of mRNAs encoding the NF-kappaB components c-Rel and KBF1 in these cells
Yoshida et al., Invest Ophthalmol Vis Sci 1998 (Anoxia...) : Role of NF-kappaB mediated interleukin-8 expression in intraocular neovascularization
Spiecker et al., J Leukoc Biol 1998 : The activation of NF-kappaB involves the phosphorylation and degradation of its cytoplasmic inhibitor IkappaB-alpha by 26S proteasomes
Lahdenpohja et al., J Leukoc Biol 1998 : We have analyzed the effect of complete T cell activation ( anti-CD3 plus anti-CD28 ) on the activation of NF-kappaB in CD45RA+ ( naive ) and CD45RO+ ( memory/effector ) T cells
Boland et al., J Biol Chem 1998 : Furthermore, the effect of TNF on NFkappaB activation was rapid, whereas C2-ceramide required an optimal treatment time of 1 h. Interestingly, TNF was found to increase ceramide in cells but only after a 1-h contact time
Kanakaraj et al., J Exp Med 1998 : Here we report that IL-1 mediated activation of JNK, p38, and NF-kappaB were all reduced in embryonic fibroblasts deficient in IRAK expression
Iwanaga et al., Free Radic Biol Med 1998 (Glioblastoma) : Here, we present direct evidence that NF-kappa B activated by ionizing radiation induces the expression of gamma-glutamylcysteine synthetase ( gamma-GCS ), the rate limiting enzyme of GSH synthesis, using T98G human glioblastoma cells
Yin et al., Cell 1998 : NF-kappaB , a key regulator of the cellular inflammatory and immune response, is activated by the HTLV-I transforming and transactivating protein Tax ... Thus, Tax mediated increases in NF-kappaB nuclear translocation result from direct interactions of Tax and MEKK1 leading to enhanced IKKbeta phosphorylation of IkappaB alpha ... Thus, Tax mediated increases in NF-kappaB nuclear translocation result from direct interactions of Tax and MEKK1 leading to enhanced IKKbeta phosphorylation of IkappaB alpha
De Plaen et al., Biochim Biophys Acta 1998 : We found that : ( a ) NF-kappaB is constitutively active in rat small intestine ; ( b ) PAF at a dose below that causing shock and bowel necrosis enhances DNA binding activity of NF-kappaB within 30 min after injection ; activated NF-kappaB contains predominantly p50 subunits ; ( c ) immunohistochemistry showed that PAF induced translocation of p50 into the nucleus of cells of the lamina propria, as well as of the epithelium ; and ( d ) the effect of PAF is abrogated by neutrophil depletion, suggesting a role of neutrophils in NF-kappaB activation
Chu et al., J Biol Chem 1998 (HTLV-I Infections) : Moreover, point mutations in Tax that ablate its IKK binding function also prevent Tax mediated activation of IKK and NF-kappaB
Liu et al., Mol Cell Biol 1998 : These results suggest that DNA-PK phosphorylation of IkappaB alpha increases its interaction with NF-kappaB to reduce NF-kappaB DNA binding properties
Parry et al., Arterioscler Thromb Vasc Biol 1998 : norLeu and TPCK also blocked IL-1beta induced MCP-1 promoter-driven reporter gene expression as well as nuclear factor (NF)-kappaB mediated reporter gene expression
Chen et al., Biochem Pharmacol 1998 : The increase in expression of mRNA for COX-2 induced by catalase may be related to the ability of catalase to stimulate cyclic AMP response element ( CRE ) and NF-IL6 transcription factors, but not nuclear factor kappa B (NF-kappaB) , for electrophoretic mobility shift assays ( EMSA ) showed that catalase enhanced nuclear factor binding to cyclic AMP response element and NF-IL6 but not to NF-kappaB
Basu et al., Biochem Biophys Res Commun 1998 (Glioma) : The role of DNA-PK , a protein kinase involved in the response to DNA damage, in the activation of NF kappa B by IR and TNF alpha was examined ... The role of DNA-PK, a protein kinase involved in the response to DNA damage, in the activation of NF kappa B by IR and TNF alpha was examined ... In M059J cells, which do not express DNA-PK, IR did not activate NF kappa B, whereas TNF alpha induction of NF kappa B was still observed ... In HeLa cells, wortmannin, an inhibitor of DNA-PK, blocked the induction of NF kappa B by IR but not by TNF alpha ... In HeLa cells, wortmannin, an inhibitor of DNA-PK , blocked the induction of NF kappa B by IR but not by TNF alpha ... These results indicate that DNA-PK participates in the activation of NF kappa B by IR but not by TNF alpha
Sen et al., Biochem Biophys Res Commun 1998 : At 100 microM, LA did not but LA-Plus inhibited H2O2 induced NF-kappaB activation and NF-kappaB directed IL-2 receptor expression ... At 150 microM LA-Plus, but not LA, inhibited TNFalpha induced NF-kappaB activation
McCarthy et al., J Biol Chem 1998 : RIP2 is a novel NF-kappaB activating and cell death inducing kinase
Samy et al., Shock 1998 (Hemorrhage...) : STAT1 activation was accompanied by Janus kinase 1 phosphorylation, whereas NF-kappaB activation was mediated by phosphorylation and rapid degradation of IkappaBalpha
Lodie et al., J Immunol 1998 : In macrophages, LPS-inducible HIV-1 gene expression is mediated in part by binding of NF-kappa B to identical tandem binding sites located within the long terminal repeat ( LTR )
Declercq et al., J Immunol 1998 : Remarkably, TRAF2-DN overexpression, which was found to inhibit the TNF dependent recruitment of endogenous wild-type TRAF2 to the TNF-R75 signaling complex, could neither block TNF-R55- or TNF-R75 induced NF-kappaB activation nor granulocyte/macrophage-CSF secretion ... Possibly, additional factors different from TRAF2 are involved in TNF mediated NF-kappaB activation
Ruiz-Ortega et al., J Immunol 1998 (Glomerulonephritis...) : Since NF-kappaB is involved in the regulation of MCP-1 gene, we explored whether the effects of AngII were mediated through NF-kappaB activation
Nakazato et al., Kidney Int 1998 : IL-1 treatment rapidly induced the binding activity of NF-kappa B ... In contrast, IL-4 did not affect basal and IL-1 induced NF-kappa B activities ... In contrast, IL-4 did not affect basal and IL-1 induced NF-kappa B activities ... These results suggest that simultaneous activation of NF-kappa B and NF-IL6 is essential for IL-6 gene expression and that IL-1 and IL-4 cooperatively stimulate MC IL-6 production through their synergistic activation of NF-IL6
Reddy et al., Proc Soc Exp Biol Med 1998 : These data reveal that collagenase gene expression can be regulated by the impairment of IL-1 stimulated NF-kappaB , STAT3, and AP-1 activities, and can highlight a possible molecular mechanism for the anti-inflammatory effects of glucocorticoids
Ishizuka et al., Clin Exp Immunol 1998 : These findings suggest that ICAM-1 or ELAM-1 expression of HUVEC stimulated via TXA2 receptors is augmented by induction of NF-kappaB and AP-1 binding activity through the PKC system, and that VCAM-1 expression is augmented by induction of NF-kappaB binding activity ... These findings suggest that ICAM-1 or ELAM-1 expression of HUVEC stimulated via TXA2 receptors is augmented by induction of NF-kappaB and AP-1 binding activity through the PKC system, and that VCAM-1 expression is augmented by induction of NF-kappaB binding activity ... These findings suggest that ICAM-1 or ELAM-1 expression of HUVEC stimulated via TXA2 receptors is augmented by induction of NF-kappaB and AP-1 binding activity through the PKC system, and that VCAM-1 expression is augmented by induction of NF-kappaB binding activity
Quay et al., Am J Respir Cell Mol Biol 1998 : To investigate the role of NF-kappaB in the particulate induced IL-6 response, we exposed human airway epithelial cells ( BEAS-2B ) to ROFA in vitro
Newton et al., Eur J Biochem 1998 (Inflammation) : Effect of dexamethasone on interleukin-1beta-(IL-1beta) induced nuclear factor-kappaB (NF-kappaB) and kappaB dependent transcription in epithelial cells ... Similarly, BEAS-2B cells showed no effect of dexamethasone on IL-1beta induced NF-kappaB ( p50/p65 ) ... We, therefore, conclude that in these epithelial cells, the repressive effects of glucocorticoids are not mediated by up-regulation of I kappaB alpha, decreased p50/p65 gene expression or inhibition of NF-kappaB DNA binding
Stehlik et al., J Exp Med 1998 : Indeed, overexpression of IkappaBalpha , an inhibitor of NF-kappaB , suppresses the induced expression of iap genes and sensitizes ECs to TNF-alpha induced apoptosis
Foxwell et al., Proc Natl Acad Sci U S A 1998 (Arthritis, Rheumatoid) : Efficient adenoviral infection with IkappaB alpha reveals that macrophage tumor necrosis factor alpha production in rheumatoid arthritis is NF-kappaB dependent ... This permitted us to show, by using adenoviral transfer of IkappaB alpha , the natural inhibitor of NF-kappaB , that induction of TNFalpha in normal human macrophages by lipopolysaccharide, but not by some other stimuli, was inhibited by 80 %
Staels et al., Nature 1998 (Coronary Disease...) : This inhibition of cyclooxygenase-2 induction occurs transcriptionally as a result of PPARalpha repression of NF-kappaB signalling
Schwager et al., Vet Immunol Immunopathol 1998 (Ascorbic Acid Deficiency...) : The results suggest that ascorbate levels exert an early effect on immune homeostasis via reactive oxygen intermediates ( ROI ) -dependent expression of interleukin genes, since the transcription factor NF-kappa B is sensitive to ROI and regulates the expression of interleukin genes
Li et al., FASEB J 1998 : TNF-alpha activated binding of nuclear factor kappaB (NF-kappaB) to its targeted DNA sequence and stimulated degradation of I-kappaBalpha, an NF-kappaB inhibitory protein
Nicot et al., J Virol 1998 : Cytoplasmic forms of human T-cell leukemia virus type 1 Tax induce NF-kappaB activation ... In contrast, they activated NF-kappaB reporters, suggesting that activation of NF-kappaB by Tax occurs in the cytoplasm
Hehner et al., J Biol Chem 1998 : Tumor necrosis factor-alpha induced cell killing and activation of transcription factor NF-kappaB are uncoupled in L929 cells
Zhong et al., Mol Cell 1998 : The transcriptional activity of NF-kappa B is stimulated upon phosphorylation of its p65 subunit on serine 276 by protein kinase A (PKA) ... Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300 ... Therefore, PKA regulates the transcriptional activity of NF-kappa B by modulating its interaction with CBP/p300
Layé et al., FEBS Lett 1998 : Human/mouse interleukin-1 receptor/receptor accessory protein interactions in IL-1beta induced NFkappaB activation
Greenberg et al., Alcohol 1998 : ETOH does not directly inhibit NFkappaB or AP-1, in vivo, but rather inhibits LPS induced activation of the MEKK/MAP kinase system and inhibition of inhibitory protein IkappaBalpha required for formation of AP-1 and NFkappaB, respectively ... ETOH does not directly inhibit NFkappaB or AP-1, in vivo, but rather inhibits LPS induced activation of the MEKK/MAP kinase system and inhibition of inhibitory protein IkappaBalpha required for formation of AP-1 and NFkappaB, respectively
Chen et al., J Biol Chem 1998 : Stimulation of cells for 1 h with LPS caused activation of nuclear factor (NF)-kB in the nuclei as detected by the formation of a NF-kB-specific DNA-protein complex ; this effect was inhibited by genestein, D609, propranolol, or Ro 31-8220 or by PKCeta antisense oligonucleotides, but not by long term TPA treatment
Jeon et al., Biochem Mol Biol Int 1998 : Treatment of DEX to RAW 264.7 cells induced a dose related inhibition of NF-kappa B/Rel in chloramphenicol acetyltransferase activity, while NF-IL6 or Oct activation was not affected by DEX ... These results suggest that DEX may inhibit iNOS gene expression by a mechanism involving the blockade of LPS induced nuclear translocation of NF-kappa B/Rel
Wong et al., Circulation 1998 (Cardiomyopathy, Dilated...) : Induction of cyclooxygenase (COX) , the enzyme responsible for generating prostanoids, requires activation of nuclear factor-kappaB (NF-kappaB) ... The aim of the present study was to determine the expression of COX-2 and activation of NF-kappaB in the failing human heart ... We demonstrate induction of COX-2 and activation of NF-kappaB in the myocardium of failing human hearts
Lin et al., Cancer Res 1998 : Brefeldin A, an agent that disrupts protein transport from the endoplasmic reticulum to the Golgi, induces the expression of GRP78 and the activation of nuclear factor (NF)-kappaB in cells
Lo et al., J Trauma 1998 (Multiple Trauma) : To elucidate the role of NFkappaB in LPS induced COX-2 gene activation, Mphi were exposed to LPS in the presence of an NFkappaB inhibitor, TPCK ... In addition, LPS induced NFkappaB activity was attenuated by these agents ... Inhibition of NFkappaB with TPCK reduced COX-2 but not COX-1 gene expression and decreased PGE2 production by LPS stimulated Mphi ... Inhibition of NFkappaB with TPCK reduced COX-2 but not COX-1 gene expression and decreased PGE2 production by LPS stimulated Mphi ... Our data indicate that COX-2 gene expression by LPS stimulated Mphi is dependent on NFkappaB
Zetterström et al., Eur Cytokine Netw 1998 (Fever) : Interleukin-1 mediated febrile responses in mice and interleukin-1 beta activation of NFkappaB in mouse primary astrocytes, involves the interleukin-1 receptor accessory protein ... Interleukin-1 mediated febrile responses in mice and interleukin-1 beta activation of NFkappaB in mouse primary astrocytes, involves the interleukin-1 receptor accessory protein ... With the aim of investigating the importance of the IL-1RAcP in IL-1 signalling, IL-1alpha and IL-1beta induced febrile responses and IL-1beta mediated activation of NFkappaB in primary astrocyte cultures were examined using IL-1RAcP-deficient ( IL-1RAcP KO ) and wild type mice, respectively ... Furthermore, it was shown that rhIL-1beta activated, in a concentration dependent manner, nuclear translocation of the transcriptional nuclear factor kappa B (NFkappaB) in primary astrocyte cultures prepared from wild type mice, whereas no IL-1beta induced translocation of NFkappaB could be detected in cultures prepared from IL-1RAcP-deficient mice, as revealed by electrophoretic mobility shift assay ( EMSA )
Galve-Roperh et al., J Neurochem 1998 : To clarify further the role of NF-kappaB in NGF synthesis, electrophoretic mobility shift assays were performed with an NF-kappaB site from the NGF promoter ... The absence of significant binding of NF-kappaB to the NGF gene promoter indicates the existence of an indirect role of NF-kappaB in the regulation of NGF synthesis
Rusyn et al., Carcinogenesis 1998 : Moreover, these findings support the hypothesis that Kupffer cells play a pivotal role in peroxisome proliferator induced hepatocyte proliferation through rapid NF-kappaB activation and subsequent induction of TNF alpha production
Sakitani et al., Genes Cells 1998 : Transfection of C/EBPbeta liver enriched activator protein (LAP) or NF-kappaB ( RelA + p50 ) activated the iNOS promoter, and transfection of LAP and NF-kappaB further activated it synergistically ... Transfection of C/EBPbeta liver enriched activator protein (LAP) or NF-kappaB ( RelA + p50 ) activated the iNOS promoter, and transfection of LAP and NF-kappaB further activated it synergistically
Grammer et al., J Immunol 1998 (Burkitt Lymphoma) : By contrast, induction of apoptosis, activation of NF-kappaB , generation of granulocyte-macrophage CSF, and up-regulation of CD54, MHC class II, and CD95 were unaffected by the TRAF3 dominant negative ... By contrast, induction of apoptosis, activation of NF-kappaB , generation of granulocyte-macrophage CSF , and up-regulation of CD54, MHC class II, and CD95 were unaffected by the TRAF3 dominant negative ... By contrast, induction of apoptosis, activation of NF-kappaB , generation of granulocyte-macrophage CSF, and up-regulation of CD54, MHC class II , and CD95 were unaffected by the TRAF3 dominant negative ... By contrast, induction of apoptosis, activation of NF-kappaB , generation of granulocyte-macrophage CSF, and up-regulation of CD54 , MHC class II, and CD95 were unaffected by the TRAF3 dominant negative
Sitrin et al., J Immunol 1998 (Leukemia, Monocytic, Acute) : Mononuclear phagocytes are exposed to fibrinous provisional matrix throughout migration into inflammatory foci, so this study was undertaken to determine whether fibrinogen triggers activation of NF-kappa B transcription factors ... NF-kappa B activation, minimal in unstimulated cells, was substantially up-regulated by fibrinogen ... Blocking mAbs against CD18 and CD11b abrogated fibrinogen induced NF-kappa B activation
DeMeester et al., Shock 1998 : Treatment with PDTC attenuated LPS induced NF-kappaB activity and endothelial cell death when added prior to LPS
Faure et al., Am J Physiol 1998 : Analysis by electrophoretic mobility shift assay of the activation of the transcription factor nuclear factor-kappaB (NF-kappaB) involved in NOS-II induction demonstrated that LPS alone or combined with IFN-gamma induced NF-kappaB binding
Lee et al., Proc Natl Acad Sci U S A 1998 : We conclude that IKK-alpha and IKK-beta can mediate the NF-kappaB inducing activity of MEKK1
Jobin et al., Gut 1998 : The role of NF-kappa B in ICAM-1 gene regulation was therefore examined by using different proteasome inhibitors in tumour necrosis factor alpha (TNF-alpha) stimulated IEC-6 rat intestinal epithelial cells ... Blocking TNF-alpha induced I kappa B alpha degradation with proteasome inhibitors reduced TNF-alpha induced NF-kappa B activation and ICAM-1 gene induction and notably decreased MOLT-4 cell adhesion without affecting Jun N-terminal kinase ( JNK/SAPK ) activity or de novo protein synthesis ... TNF-alpha induction of ICAM-1 expression is mediated by the transcription factor NF-kappa B and can be inhibited by blocking I kappa B alpha degradation
Ling et al., Blood 1998 (Carcinoma, Renal Cell...) : Impaired activation of NFkappaB in T cells from a subset of renal cell carcinoma patients is mediated by inhibition of phosphorylation and degradation of the inhibitor, IkappaBalpha
Uhlik et al., J Biol Chem 1998 : NF-kappaB inducing kinase and IkappaB kinase participate in human T-cell leukemia virus I Tax mediated NF-kappaB activation ... Consistently, inactive forms of either IKKs or NIK attenuate Tax mediated NF-kappaB activation ... Therefore, Tax activates NF-kappaB by targeting cellular signaling molecules, including both IKKs and NIK
Adachi et al., Immunity 1998 : Furthermore, IL-18 induced activation of NF-kappaB and c-Jun N-terminal kinase (JNK) is blocked in MyD88-/- Th1 developing cells
Fiedler et al., Am J Respir Cell Mol Biol 1998 : Inhibition of TNF-alpha induced NF-kappaB activation and IL-8 release in A549 cells with the proteasome inhibitor MG-132 ... The working hypothesis of the studies described herein was that inhibition of proteasome mediated IkappaB degradation would inhibit TNF-alpha induced nuclear factor-kappaB (NF-kappaB) activation , interleukin-8 (IL-8) gene transcription, and IL-8 protein release in A549 cells
Feinstein et al., Nitric Oxide 1997 : Activation of C6 cell NFkappaB by LPS plus CM required IkappaB degradation by the 20S proteasome, since NOS-2 expression was blocked by a selective proteasome inhibitor
Thome et al., Curr Biol 1998 : TNFR-1 recruits and assembles a signaling complex containing a number of death domain ( DD ) -containing proteins, including the adaptor protein TRADD and the serine/threonine kinase RIP, which mediates TNF induced NF-kappa B activation ... CARDIAK interacted with the TNFR associated factors TRAF-1 and TRAF-2, and a dominant negative form of TRAF-2 inhibited CARDIAK induced NF-kappa B activation
Sylla et al., Proc Natl Acad Sci U S A 1998 : The Epstein-Barr virus oncoprotein latent infection membrane protein 1 ( LMP1 ) is a constitutively aggregated pseudo-tumor necrosis factor receptor (TNFR) that activates transcription factor NF-kappaB through two sites in its C-terminal cytoplasmic domain ... TNFRI has been recently shown to activate NF-kappaB through association with TRADD, RIP, and TRAF2 ; activation of the NF-kappaB inducing kinase (NIK) ; activation of the IkappaB alpha kinases ( IKKalpha and IKKbeta ) ; and phosphorylation of IkappaB alpha ... IkappaB alpha phosphorylation on Ser-32 and Ser-36 is followed by its degradation and NF-kappaB activation ... In this report, we show that NF-kappaB activation by LMP1 or by each of its effector sites is mediated by a pathway that includes NIK, IKKalpha, and IKKbeta ... Dominant negative mutants of NIK, IKKalpha , or IKKbeta substantially inhibited NF-kappaB activation by LMP1 or by each of its effector sites ... Dominant negative mutants of NIK, IKKalpha, or IKKbeta substantially inhibited NF-kappaB activation by LMP1 or by each of its effector sites
Pyatt et al., Toxicology 1998 : We demonstrate that 0.1-0.5 microM DMDTC inhibits TNF-alpha induced activation of NF-kappaB in primary human CD4+ T cells
Umansky et al., Eur J Immunol 1998 (Inflammation) : This provides a mechanism for a self amplifying signal in the inflammatory response, since the inducible NO synthase in endothelial cells is regulated by NF-kappaB ... High doses of NO impaired the TNF-alpha induced DNA binding activity of NF-kappaB
Vollebregt et al., FEBS Lett 1998 : The neutrophil NADPH oxidase inhibitor diphenylene iodonium, catalase , and other oxidant scavengers did not inhibit NF-kappaB activation, and no activation was seen with added hydrogen peroxide
Geleziunas et al., Mol Cell Biol 1998 (Cell Transformation, Viral...) : Human T-cell leukemia virus type 1 Tax induction of NF-kappaB involves activation of the IkappaB kinase alpha (IKKalpha) and IKKbeta cellular kinases ... Human T-cell leukemia virus type 1 Tax induction of NF-kappaB involves activation of the IkappaB kinase alpha (IKKalpha) and IKKbeta cellular kinases ... Human T-cell leukemia virus type 1 Tax induction of NF-kappaB involves activation of the IkappaB kinase alpha (IKKalpha) and IKKbeta cellular kinases ... Tax expression promotes N-terminal phosphorylation and degradation of IkappaB alpha , a principal cytoplasmic inhibitor of NF-kappaB ... Similarly, a kinase-deficient mutant of NIK ( NF-kappaB inducing kinase ), which represents an upstream kinase in the TNF-alpha and IL-1 signaling pathways leading to IKKalpha and IKKbeta activation, blocks Tax induction of NF-kappaB ... However, plasma membrane-proximal elements in these proinflammatory cytokine pathways are apparently not involved since dominant negative mutants of the TRAF2 and TRAF6 adaptors, which effectively block signaling through the cytoplasmic tails of the TNF-alpha and IL-1 receptors, respectively, do not inhibit Tax induction of NF-kappaB ... However, plasma membrane-proximal elements in these proinflammatory cytokine pathways are apparently not involved since dominant negative mutants of the TRAF2 and TRAF6 adaptors, which effectively block signaling through the cytoplasmic tails of the TNF-alpha and IL-1 receptors, respectively, do not inhibit Tax induction of NF-kappaB ... The pathological alteration of this cytokine pathway leading to NF-kappaB activation by Tax may play a central role in HTLV-1 mediated transformation of human T cells, clinically manifested as the adult T-cell leukemia ... However, plasma membrane-proximal elements in these proinflammatory cytokine pathways are apparently not involved since dominant negative mutants of the TRAF2 and TRAF6 adaptors, which effectively block signaling through the cytoplasmic tails of the TNF-alpha and IL-1 receptors, respectively, do not inhibit Tax induction of NF-kappaB
Lin et al., Mol Cell Biol 1998 : Although LPS also activates NF-kappaB , it poorly induces germ line gamma1 transcripts
Jeppson et al., J Immunol 1998 : These data indicate that both IL-4 and anti-CD40 are required to induce NF-kappa B activation and IL-6 transcription and production, and implicate these events in a signaling pathway augmenting IgE production in human B lymphocytes ... These data indicate that both IL-4 and anti-CD40 are required to induce NF-kappa B activation and IL-6 transcription and production, and implicate these events in a signaling pathway augmenting IgE production in human B lymphocytes
Marks-Konczalik et al., J Biol Chem 1998 : The involvement of AP-1 and NF-kappaB transcription factors in cytokine mediated induction of human inducible nitric oxide synthase ( hiNOS ) promoter activity was examined
Kurokouchi et al., J Bone Miner Res 1998 (Bone Neoplasms...) : TNF-alpha increases expression of IL-6 and ICAM-1 genes through activation of NF-kappaB in osteoblast-like ROS17/2.8 cells ... We thus examined the effect of TNF-alpha on the activation of NF-kappaB in rat osteoblast-like osteosarcoma cells ( ROS17/2.8 ) ... Electrophoretic mobility shift assay revealed that the activation of the p50-p65 heterodimer NF-kappaB was induced by TNF-alpha as early as 15 minutes followed by a persistent activation for 48 h ... These results suggest that the rapid activation of NF-kappaB by TNF-alpha is mainly due to the nuclear translocation of NF-kappaB pre existing in cytosol, and that the subsequent increase in the expression of p50 and p65 may result in the persistent activation of NF-kappaB during TNF-alpha stimulation ... These results suggest that the rapid activation of NF-kappaB by TNF-alpha is mainly due to the nuclear translocation of NF-kappaB pre existing in cytosol, and that the subsequent increase in the expression of p50 and p65 may result in the persistent activation of NF-kappaB during TNF-alpha stimulation ... An antioxidant, N-acetyl-L-cysteine, significantly attenuated the TNF-alpha dependent increase in these mRNAs, and simultaneously reduced the activation of NF-kappaB by TNF-alpha , indicating that NF-kappaB mediates the TNF-alpha dependent expression of IL-6 and ICAM-1 in ROS17/2.8 cells ... An antioxidant, N-acetyl-L-cysteine, significantly attenuated the TNF-alpha dependent increase in these mRNAs, and simultaneously reduced the activation of NF-kappaB by TNF-alpha, indicating that NF-kappaB mediates the TNF-alpha dependent expression of IL-6 and ICAM-1 in ROS17/2.8 cells ... An antioxidant, N-acetyl-L-cysteine, significantly attenuated the TNF-alpha dependent increase in these mRNAs, and simultaneously reduced the activation of NF-kappaB by TNF-alpha, indicating that NF-kappaB mediates the TNF-alpha dependent expression of IL-6 and ICAM-1 in ROS17/2.8 cells ... These results suggest that the activation of NF-kappaB by TNF-alpha may play an important role in the production of cytokines and cell adhesion molecules from osteoblasts, leading to the promotion of bone resorption and inflammation
Sodhi et al., Immunol Lett 1998 : It was observed that cisplatin induced NF-kappaB translocation involves serine/threonine phosphatases 1/2A, protein tyrosine phosphatases and genestein sensitive protein tyrosine kinase activities
Trebilcock et al., Mech Ageing Dev 1998 : Our analyses of the induction of nuclear factor-kappaB (NFkappaB) in activated memory ( CD45RO+ ) and naive ( CD45RA+ ) T cell subsets from young and elderly donors has demonstrated that, regardless of donor age, memory T cells are not significantly altered in their responsiveness to TNF-alpha mediated induction of NFkappaB
Coscoy et al., Virology 1998 (Cell Transformation, Neoplastic...) : Therefore, Tax induced cell transformation in mice correlates with the degradation of IkappaB alpha and IkappaB beta and with the constitutive activation of NF-kappaB ... Therefore, Tax induced cell transformation in mice correlates with the degradation of IkappaB alpha and IkappaB beta and with the constitutive activation of NF-kappaB
Taylor et al., Biochemistry (Mosc) 1998 (Acute-Phase Reaction...) : iNOS expression requires the transcription factor NF-kappaB and is down-regulated by steroids, TGF-beta, the heat shock response, p53 , and nitric oxide ( NO ) itself ... iNOS expression requires the transcription factor NF-kappaB and is down-regulated by steroids, TGF-beta, the heat shock response, p53, and nitric oxide ( NO ) itself
Shames et al., Am J Physiol 1998 : In saline pretreated rats, LPS caused a rapid decrease in myocardial IkappaB-alpha protein levels, activation of NF-kappaB , and increased TNF-alpha production ... In saline pretreated rats, LPS caused a rapid decrease in myocardial IkappaB-alpha protein levels, activation of NF-kappaB , and increased TNF-alpha production ... In saline pretreated rats, LPS caused a rapid decrease in myocardial IkappaB-alpha protein levels, activation of NF-kappaB , and increased TNF-alpha production
Yamit-Hezi et al., EMBO J 1998 : Moreover, inhibition of TAFII105 activity by overexpression of a dominant negative mutant of TAFII105 decreased NF-kappaB transcriptional activity and severely reduced cell survival in response to TNF-alpha ... These results suggest that TAFII105 is involved in activation of anti-apoptotic genes by NF-kappaB
de Moissac et al., J Biol Chem 1998 : Bcl-2 activates the transcription factor NFkappaB through the degradation of the cytoplasmic inhibitor IkappaBalpha ... Nuclear DNA binding activity of NFkappaB was significantly increased in myocytes expressing Bcl-2, with a concomitant reduction in IkappaBalpha protein level
Xu et al., Brain Res Mol Brain Res 1998 (Spinal Cord Injuries) : Methylprednisolone inhibition of TNF-alpha expression and NF-kB activation after spinal cord injury in rats
Mayne et al., Neuroimmunomodulation 1998 (AIDS Dementia Complex...) : TNF-alpha induction was NF-kappaB dependent and was eliminated by inhibiting protein kinase A, phospholipase C and protein tyrosine kinase activity
Kennedy et al., Am J Respir Cell Mol Biol 1998 (Asthma...) : Mucin significantly reduced IL-8 secretion, and ceruloplasmin significantly increased IL-8 secretion and activation of NF-kappaB ... Mucin significantly reduced IL-8 secretion, and ceruloplasmin significantly increased IL-8 secretion and activation of NF-kappaB
Lawson et al., Hepatology 1998 : Administration of uridine ( 1,000 mg/kg ) did not reduce plasma levels of TNF-alpha and KC, NF-kappaB activation , or polymorphonuclear leukocyte sequestration, but attenuated apoptosis by 90 % to 94 %
Wang et al., Science 1998 : Tumor necrosis factor alpha (TNF-alpha) binding to the TNF receptor (TNFR) potentially initiates apoptosis and activates the transcription factor nuclear factor kappa B (NF-kappaB) , which suppresses apoptosis by an unknown mechanism
Gu et al., FASEB J 1998 : Overexpression of BRE inhibited TNF induced NFkappaB activation, indicating that the interaction of BRE protein with the cytoplasmic region of p55 TNF receptor may modulate signal transduction by TNF-alpha ... Overexpression of BRE inhibited TNF induced NFkappaB activation, indicating that the interaction of BRE protein with the cytoplasmic region of p55 TNF receptor may modulate signal transduction by TNF-alpha
Harhaj et al., J Biol Chem 1998 : The interleukin-2 gene is regulated by NF-kappaB among other transcription factors, in part, via a CD28 responsive element (CD28RE) present in the IL-2 promoter ... Enhanced activation of NF-kappaB by CD28 is mediated by rapid phosphorylation and proteasome mediated degradation of the NF-kappaB inhibitory proteins IkappaB alpha and IkappaB beta, which allows for accelerated nuclear expression of the liberated NF-kappaB ... These findings suggest that cellular signaling pathways initiated at the TCR and CD28 converge at or upstream of IKK , resulting in more robust kinase activity and enhanced and prolonged NF-kappaB activation
Guerrini et al., FEBS Lett 1998 : NF-kappaB binding activity was induced at early times by LPS treatment, but its DNA binding activity disappeared after 84 h of LPS treatment ... Accumulation of IkappaB alpha protein in the nucleus correlated with the disappearance of NF-kappaB activity at 72, 84 and 96 h, and treatment of nuclear extracts of 72-96 h LPS treated cells with Na-deoxycholate restored NF-kappaB binding activity
Lin et al., Mol Cell Biol 1998 : Such a NIK-T559A mutant also dominantly interferes with TNF-alpha induction of NF-kappaB ... When expressed alone, this C-terminal NIK fragment functions as a potent inhibitor of TNF-alpha mediated induction of NF-kappaB and alone is sufficient to disrupt the physical association of NIK and IKKalpha
Manna et al., J Immunol 1998 : Pretreatment of cells with IL-13 blocked TNF induced NF-kappa B activation , nuclear translocation of p65 subunit, and degradation of I kappa B alpha ... IL-13 also inhibited NF-kappa B activation by LPS, okadaic acid, H2O2, and ceramide ... IL-13 also inhibited NF-kappa B activation by LPS , okadaic acid, H2O2, and ceramide ... TNF induced NF-kappa B-dependent gene transcription was also blocked by IL-13 ... TNF induced NF-kappa B-dependent gene transcription was also blocked by IL-13 ... Thus, overall, these results demonstrate that IL-13 is a potent inhibitor of TNF mediated activation of NF-kappa B , AP-1, and apoptosis, which may contribute to its previously described immunosuppressive and anti-inflammatory effects
Manna et al., J Immunol 1998 (Glioma...) : Electrophoretic mobility shift assay showed that alpha-MSH completely abolished TNF mediated NF-kappa B activation in a dose- and time dependent manner ... It also suppressed NF-kappa B activation induced by LPS , okadaic acid, and ceramide ... This correlated with suppression of NF-kappa B-dependent reporter gene expression induced by TNF ... Similarly, addition of membrane-permeable dibutyryl cAMP, like alpha-MSH, suppressed TNF induced NF-kappa B activation
Delude et al., J Immunol 1998 : An analysis of LPS induced NF-kappaB activity in heterokaryons derived from polyethylene glycol fused cell lines indicated that recessive mutations in genes encoding components of the LPS signaling pathway accounted for the signaling defects
Los et al., J Immunol 1998 : Hormone induced Tax activation induces a long lasting activation of NF-kappaB , which is a major target of reactive oxygen intermediates
Lazaar et al., J Immunol 1998 : In addition, inhibition of calcium influx inhibited both CD40 mediated NF-kappaB activation and enhancement of IL-6 secretion ... These results delineate a potentially important CD40 mediated signal-transduction pathway in ASM, involving protein tyrosine kinase dependent calcium mobilization, NF-kappaB activation, and IL-6 production
Zen et al., Exp Cell Res 1998 : Lipopolysaccharide induced NF-kappaB activation in human endothelial cells involves degradation of IkappaBalpha but not IkappaBbeta ... Lipopolysaccharide induced NF-kappaB activation in human endothelial cells involves degradation of IkappaBalpha but not IkappaBbeta ... We studied the signal transduction pathways involved in NF-kappaB activation and the induction of the cytoprotective A20 gene by lipopolysaccharide (LPS) in human umbilical vein endothelial cells ( HUVEC ) ... The proteasome inhibitor N-acetyl-leucinyl-leucinyl-norleucinal-H ( ALLN ) and the tyrosine kinase inhibitor herbimycin A ( HMA ) blocked A20 mRNA expression and partially inhibited NF-kappaB DNA binding activity induced by LPS treatment ... LPS induced IkappaBalpha degradation was inhibited by ALLN, confirming that ALLN inhibits NF-kappaB activation by preventing IkappaBalpha degradation
McDonald et al., Biochemistry 1998 : To circumvent this problem, we developed an alternative procedure which allowed us to show that in neutrophils, LPS and TNFalpha induce a NF-kappaB DNA binding activity which essentially consists of p50/RelA dimers, and that IFNgamma promotes the binding of STAT1 homodimers to the IFNgamma response region of the FcgammaRI promoter ... To circumvent this problem, we developed an alternative procedure which allowed us to show that in neutrophils, LPS and TNFalpha induce a NF-kappaB DNA binding activity which essentially consists of p50/RelA dimers, and that IFNgamma promotes the binding of STAT1 homodimers to the IFNgamma response region of the FcgammaRI promoter ... To circumvent this problem, we developed an alternative procedure which allowed us to show that in neutrophils, LPS and TNFalpha induce a NF-kappaB DNA binding activity which essentially consists of p50/RelA dimers, and that IFNgamma promotes the binding of STAT1 homodimers to the IFNgamma response region of the FcgammaRI promoter
Rothwarf et al., Nature 1998 : An IKK-gamma carboxy-terminal truncation mutant that still binds IKK-beta blocks the activation of IKK and NF-kappaB
Ko et al., J Neurochem 1998 : NMDA treatment also enhanced the DNA binding activity of nuclear NF-kappaB in a Ca2+ dependent manner
Darville et al., Diabetologia 1998 : Site-mutation analysis showed that both the distal and proximal NF-kappaB and GAS are necessary for IL-1beta induced iNOS expression in RINm5F cells ... In these cells IFN-gamma potentiation is mostly mediated by GAS and ISRE, suggesting a role for the IFN-gamma induced transcription factors Stat1alpha ( which binds GAS ) and IRF-1 ( which binds ISRE ), which may cooperate with NF-kappaB induced by IL-1beta for iNOS activation
Xu et al., Am J Physiol 1998 : ActD did not affect TNF-alpha induced hepatocyte NF-kappaB activation but decreased NF-kappaB dependent gene expression
Yamada et al., Hepatology 1998 : Lack of TNFR-2 had little effect on NF-kappaB and STAT3 binding, and no effect in interleukin-6 production after PH, but caused a delay in AP-1 and C/EBP binding and in the expression of c-jun and c-myc messenger RNA ( mRNA ) ... We conclude that TNFR-1 , but not TNFR-2, is necessary for liver regeneration, and that NF-kappaB and STAT3 binding are activated by signals transduced by TNFR-1 ... We conclude that TNFR-1, but not TNFR-2 , is necessary for liver regeneration, and that NF-kappaB and STAT3 binding are activated by signals transduced by TNFR-1
Bond et al., FEBS Lett 1998 (Inflammation) : NF-kappaB binding was rapidly induced by IL-1alpha or TNF-alpha but was neither induced nor potentiated by bFGF or PDGF ... NF-kappaB binding was rapidly induced by IL-1alpha or TNF-alpha but was neither induced nor potentiated by bFGF or PDGF ... Using a recently developed adenovirus that overexpresses the inhibitory subunit, IkappaB alpha, we demonstrated an absolute requirement for NF-kappaB in upregulation of MMP-9 ... Activation of NF-kappaB binding by inflammatory cytokines was therefore necessary but not sufficient for synergistic upregulation of MMP-9
Agresti et al., Eur J Neurosci 1998 : The existence of a synergism between IFN-gamma and TNF-alpha in stimulating IRF-1 expression at the transcriptional level was supported by IRF-1 promoter analysis : IFN-gamma directly induced the binding of STAT-1 homodimers to the GAS element, while NF-kappaB binding to the kappaB sequence was activated by TNF-alpha only after IFN-gamma treatment ... The existence of a synergism between IFN-gamma and TNF-alpha in stimulating IRF-1 expression at the transcriptional level was supported by IRF-1 promoter analysis : IFN-gamma directly induced the binding of STAT-1 homodimers to the GAS element, while NF-kappaB binding to the kappaB sequence was activated by TNF-alpha only after IFN-gamma treatment ... The existence of a synergism between IFN-gamma and TNF-alpha in stimulating IRF-1 expression at the transcriptional level was supported by IRF-1 promoter analysis : IFN-gamma directly induced the binding of STAT-1 homodimers to the GAS element, while NF-kappaB binding to the kappaB sequence was activated by TNF-alpha only after IFN-gamma treatment ... The existence of a synergism between IFN-gamma and TNF-alpha in stimulating IRF-1 expression at the transcriptional level was supported by IRF-1 promoter analysis : IFN-gamma directly induced the binding of STAT-1 homodimers to the GAS element, while NF-kappaB binding to the kappaB sequence was activated by TNF-alpha only after IFN-gamma treatment
Wang et al., Prostaglandins Leukot Essent Fatty Acids 1998 : These results provide convincing evidence that NF-kB may mediate the IL-1beta stimulation of PGHS-2 gene expression as well as the dexamethasone inhibition of the IL-1beta induction process in WISH cells ... These results provide convincing evidence that NF-kB may mediate the IL-1beta stimulation of PGHS-2 gene expression as well as the dexamethasone inhibition of the IL-1beta induction process in WISH cells
Craxton et al., J Immunol 1998 (Lymphoma, B-Cell) : We have investigated the activation of the p38 MAPK pathway in response to CD40 engagement in multiple B cell lines and in human tonsillar B cells to define the role of p38 MAPK in proliferation, NF-kappaB activation and gene expression ... SB203580 also significantly reduced expression of a reporter gene driven by a minimal promoter containing four NF-kappaB elements, indicating a requirement for the p38 MAPK pathway in CD40 induced NF-kappaB activation ... However, CD40 mediated NF-kappaB binding was not affected by SB203580, suggesting that NF-kappaB may not be a direct target for the CD40 induced p38 MAPK pathway
Thommesen et al., J Immunol 1998 : We show that TNF induced activation of NF-kappaB was inhibited by the well-known selective inhibitors of cytosolic phospholipase A2 (cPLA2) : the trifluoromethyl ketone analogue of arachidonic acid ( AACOCF3 ) and methyl arachidonyl fluorophosphate ... We show that TNF induced activation of NF-kappaB was inhibited by the well-known selective inhibitors of cytosolic phospholipase A2 (cPLA2) : the trifluoromethyl ketone analogue of arachidonic acid ( AACOCF3 ) and methyl arachidonyl fluorophosphate ... The trifluoromethyl ketone analogue of eicosapentaenoic acid ( EPACOCF3 ) also suppressed TNF induced NF-kappaB activation and inhibited in vitro cPLA2 enzyme activity with a similar potency as AACOCF3 ... The trifluoromethyl ketone analogue of eicosapentaenoic acid ( EPACOCF3 ) also suppressed TNF induced NF-kappaB activation and inhibited in vitro cPLA2 enzyme activity with a similar potency as AACOCF3 ... The arachidonyl methyl ketone analogue ( AACOCH3 ) and the eicosapentanoyl analogue ( EPACHOHCF3 ), which both failed to inhibit cPLA2 enzyme activity in vitro, had no effect on TNF induced NF-kappaB activation ... TNF induced NF-kappaB activation was also strongly reduced in cells stimulated in the presence of the secretory PLA2 ( sPLA2 ) inhibitors 12-epi-scalaradial and LY311727 ... Activation of NF-kappaB by IL-1beta was markedly less sensitive to both cPLA2 and sPLA2 inhibitors
Oertli et al., J Immunol 1998 : Inhibition of NF-kappaB with the serine protease inhibitor N-tosyl-L-phenylalanine chloromethyl ketone blocked the HA-mediated expression of ICAM-1 and VCAM-1 in MCT cells ... Inhibition of NF-kappaB with the serine protease inhibitor N-tosyl-L-phenylalanine chloromethyl ketone blocked the HA-mediated expression of ICAM-1 and VCAM-1 in MCT cells
Lindroos et al., J Immunol 1998 : These results suggest that NF-kappaB activation alone is not sufficient for up-regulation of PDGF-Ralpha
Dumais et al., J Biol Chem 1998 (Second Messenger Systems) : The involvement of NF-kappaB in the PGE2 dependent activating effect on HIV-1 transcription was further confirmed using a kappaB regulated luciferase encoding vector and by electrophoretic mobility shift assays
Sánchez-Mejorada et al., J Biol Chem 1998 : Using the specific MAPK/extracellular signal regulated kinase kinase ( MAPK kinase ) inhibitor PD98059, we found that MAPK activation is necessary for FcR dependent activation of the nuclear factor NF-kappaB
Shapiro et al., Proc Natl Acad Sci U S A 1998 : These results demonstrate induction of HIV-1 by IL-18 in a monocyte target associated with an intermediate role for TNF and IL-6, activation of p38 mitogen activated protein kinase, and nuclear translocation of NF-kappaB
Wong et al., J Biol Chem 1998 : The TRAF family of signal transducers mediates NF-kappaB activation by the TRANCE receptor ... The TRANCE receptor (TRANCE-R) , recently identified as receptor activator of NF-kappabeta ( RANK ), activates NF-kappaB , a transcription factor critical in the differentiation and activation of those cells ... In this report we identify the TNF receptor associated factor (TRAF) family of signal transducers as important components of TRANCE-R mediated NF-kappaB activation ... Dominant negative forms of TRAF2, TRAF5 , and TRAF6 and an endogenous inhibitor of TRAF2, TRAF interacting protein (TRIP), substantially inhibited TRANCE-R mediated NF-kappaB activation, suggesting a role of TRAFs in regulating DC and osteoclast function ... Dominant negative forms of TRAF2, TRAF5, and TRAF6 and an endogenous inhibitor of TRAF2 , TRAF interacting protein (TRIP), substantially inhibited TRANCE-R mediated NF-kappaB activation, suggesting a role of TRAFs in regulating DC and osteoclast function ... Dominant negative forms of TRAF2, TRAF5, and TRAF6 and an endogenous inhibitor of TRAF2, TRAF interacting protein (TRIP), substantially inhibited TRANCE-R mediated NF-kappaB activation, suggesting a role of TRAFs in regulating DC and osteoclast function ... Dominant negative forms of TRAF2, TRAF5, and TRAF6 and an endogenous inhibitor of TRAF2, TRAF interacting protein (TRIP), substantially inhibited TRANCE-R mediated NF-kappaB activation, suggesting a role of TRAFs in regulating DC and osteoclast function
Kumar et al., Oncogene 1998 : Emodin ( 3-methyl-1,6,8-trihydroxyanthraquinone ) inhibits TNF induced NF-kappaB activation, IkappaB degradation, and expression of cell surface adhesion proteins in human vascular endothelial cells
Marriott et al., J Immunol 1998 : Furthermore, CC-3052 did not increase the degradation rate of macrophage TNF-alpha transcripts nor inhibit LPS induced primary macrophage NF-kappaB activation
Liu et al., Infect Immun 1998 (Endotoxemia) : The expressed CD14 protein from this clone was functional, as indicated by NF-kappaB activation in response to LPS and fluorescein isothiocyanate-LPS binding in CHO cells stably transfected with rat CD14
Wolf et al., J Biol Chem 1998 : Downstream activation of a TATA-less promoter by Oct-2, Bob1, and NF-kappaB directs expression of the homing receptor BLR1 to mature B cells
Ding et al., J Biol Chem 1998 : A new quantitative cytometric technique, termed the ArrayScanTM, is described and used to measure NF-kappaB nuclear translocation induced by interleukin (IL)-1 and tumor necrosis factor-alpha (TNFalpha) ... A new quantitative cytometric technique, termed the ArrayScanTM, is described and used to measure NF-kappaB nuclear translocation induced by interleukin (IL)-1 and tumor necrosis factor-alpha (TNFalpha)
Kothny-Wilkes et al., J Biol Chem 1998 : IL-1 significantly induced activation of the transcription factor NFkappaB in transformed keratinocytes ... Moreover, the proteasome inhibitor MG132, which inhibits IL-1 induced NFkappaB activation, completely prevented the protective effect of IL-1
Majumder et al., J Neurosci Res 1998 (Astrocytoma) : TNF-alpha induced NFkappaB binding activity at this site was composed of RelA ( p65 ) homodimers
Li et al., Proc Natl Acad Sci U S A 1998 : Furthermore, expression of a catalytically inactive IKKbeta mutant prevented NF-kappaB activation by gamma radiation, but not by UV-C
Kelly et al., AIDS 1998 (AIDS-Related Opportunistic Infections...) : Tat and TNFalpha activated comparable levels of NF-kappaB ... Tat and TNFalpha activated comparable levels of NF-kappaB
Abu-Amer et al., J Biol Chem 1998 : In contrast, we find TNF induction of NF-kappaB in murine bone marrow macrophages ( BMMs ), is mediated, by c-Src, in a cell, and cytokine specific manner ... Consistent with the pivotal role played by c-Src in TNF induced Ikappa Balpha tyrosine phosphorylation, NF-kappaB activation, by the cytokine, is markedly delayed and reduced in c-src-/- BMMs. Underscoring the physiological significance of c-Src activation of NF-kappaB , TNF induction of IL-6, which is an NF-kappaB mediated event, is substantially diminished in c-src-/- BMMs
Born et al., J Biol Chem 1998 : In transient transfection assays, we were unable to demonstrate a role for AcPL in IL-1 induced activation of NFkappaB ... In transient transfection assays, we were unable to demonstrate a role for AcPL in IL-1 induced activation of NFkappaB ... We found that both IL-1R-rp1 and AcPL expression were required for induction of NFkappaB activity and for activation of c-Jun N-terminal kinase in response to IL-18 ... We found that both IL-1R-rp1 and AcPL expression were required for induction of NFkappaB activity and for activation of c-Jun N-terminal kinase in response to IL-18 ... We found that both IL-1R-rp1 and AcPL expression were required for induction of NFkappaB activity and for activation of c-Jun N-terminal kinase in response to IL-18
Chen et al., J Biol Chem 1998 : Taken together, these data demonstrate that nucleotides, especially UTP, can potentiate the LPS induced activation of NF-kappaB and AP-1 and of iNOS induction via a CaMK -dependent pathway and suggest that the UTP dependent up-regulation of iNOS may constitute a novel element in the inflammatory process
Yamamoto et al., J Immunol 1998 : In the present study we demonstrate that overexpression of CD27 in 293 cells induces a low level of NF-kappaB activation, and the ligation of the receptor by its corresponding ligand ( CD70 ) augments this signal dramatically ... This NF-kappaB activation signal is inhibited by dominant negative TRAF2 or intact TRAF3, indicating that TRAF2 and TRAF3 works as a mediator and an inhibitor, respectively ... This NF-kappaB activation signal is inhibited by dominant negative TRAF2 or intact TRAF3 , indicating that TRAF2 and TRAF3 works as a mediator and an inhibitor, respectively
Pelletier et al., J Immunol 1998 (Leukemia, Basophilic, Acute) : Fc epsilonRI mediated induction of TNF-alpha gene expression in the RBL-2H3 mast cell line : regulation by a novel NF-kappaB-like nuclear binding complex ... Consistent with a role for the novel NF-kappaB nuclear binding activity in TNF-alpha gene expression, deletion of several 5 ' kappaB elements in the TNF-alpha promoter abolished all measurable Fc epsilonRI dependent induction of a reporter construct
Katsuyama et al., Endocrinology 1998 (Translocation, Genetic) : Neutral SMase, IL-1beta, and TNF alpha activated NF-kappaB , as revealed by electrophoretic mobility shift assay, and its nuclear translocation, as demonstrated by immunocytochemical study ... Neutral SMase, IL-1beta , and TNF alpha activated NF-kappaB , as revealed by electrophoretic mobility shift assay, and its nuclear translocation, as demonstrated by immunocytochemical study
Webber et al., Hepatology 1998 : The results demonstrate that TNF transiently activates NF-kappaB and STAT3 and increases the proliferative response of hepatocytes to HGF or TGF- by fourfold
Tinnell et al., Int Immunol 1998 : Based on these data, a model is presented which suggests that CD23 superinduction results from STAT and NF-kappaB interaction
Norman et al., Cardiovasc Res 1998 : Nuclear factor NF-kappa B in myocardium : developmental expression of subunits and activation by interleukin-1 beta in cardiac myocytes in vitro
Bohuslav et al., J Clin Invest 1998 : In the present study, which uses mice with genetic deletions of the proteins of NF-kappaB complex, we provide data demonstrating that increased expression of the p50 subunit of NF-kappaB directly results in the downregulation of LPS induced TNF production
O'Connell et al., J Biol Chem 1998 : Phosphorylation of the IkappaB cytoplasmic inhibitors, IkappaBalpha , IkappaBbeta, and IkappaBepsilon, by these kinases triggers proteolytic degradation and the release of NF-kappaB/Rel proteins into the nucleus ... Phosphorylation of the IkappaB cytoplasmic inhibitors, IkappaBalpha, IkappaBbeta , and IkappaBepsilon, by these kinases triggers proteolytic degradation and the release of NF-kappaB/Rel proteins into the nucleus ... Phosphorylation of the IkappaB cytoplasmic inhibitors, IkappaBalpha, IkappaBbeta, and IkappaBepsilon , by these kinases triggers proteolytic degradation and the release of NF-kappaB/Rel proteins into the nucleus
Bhullar et al., J Biol Chem 1998 : This IKK activation is followed by IkappaB degradation and NF-kappaB translocation into the nucleus
Mazzeo et al., Eur J Immunol 1998 : Decreased IL-12 production and Th1 cell development by acetyl salicylic acid mediated inhibition of NF-kappaB ... Analysis of the regulation of the p40 gene promoter revealed that ASA inhibited NF-kappaB activation and binding to the p40-kappaB site in the p40 promoter, leading to transcriptional repression of the p40 gene ... These results suggest that the inhibition of NF-kappaB activation by ASA leads to down-regulation of IL-12 production and inhibition of Th1 cell development
Rüngeler et al., Planta Med 1998 : Here we show that these Sls do not influence the enzymes of the arachidonic acid pathway, but inhibit the activation of NF-kappa B
Vincenti et al., Arthritis Rheum 1998 (Arthritis, Rheumatoid) : These studies demonstrate, for the first time, a role for NF-kappaB in the induction of MMP-1 , and suggest a mechanism of NF-kappaB mediated cartilage degradation in rheumatoid arthritis
Shrivastava et al., J Virol 1998 : Stable cell transfectants expressing the HCV core protein suppressed tumor necrosis factor (TNF) induced NF-kappaB activation
Wu et al., Gene Ther 1998 (Nasopharyngeal Neoplasms) : Transcriptional activation of NF-kappa B activity by Epstein-Barr virus ( EBV ) LMP1 as a selective therapeutic strategy for EBV associated diseases
Delfino et al., Mol Endocrinol 1998 : Tumor necrosis factor-alpha , which is produced by round spermatids in the testis, increased nuclear NF-kappaB binding activity when added to Sertoli cells
Nemoto et al., Mol Cell Biol 1998 : MEKK1 is activated by tumor necrosis factor alpha (TNF-alpha) and interleukin-1 and can potentiate the stimulatory effect of TNF-alpha on IKK and NF-kappaB activation
Paludan et al., J Gen Virol 1998 (Herpes Genitalis) : HSV-2 infection and IFN-gamma stimulation of macrophages synergistically induced TNF-alpha secretion and nuclear translocation of NF-kappaB , which bound to a sequence corresponding to a kappaB site in the iNOS promoter ... The effect of the NF-kappaB activation inhibitor was not mediated through inhibition of the production of interferon regulatory factor (IRF)-1 or of TNF-alpha itself, and a possible alternative mechanism of activation of NF-kappaB through virus induced activation of the kinase PKR was also ruled out
Yorek et al., Biochem J 1998 : TNF-alpha activates nuclear factor kappaB (NF-kappaB) in 3T3-L1 adipocytes but, unlike the effect of TNF-alpha on cultured endothelial cells, NF-kappaB does not seem to contribute to the regulation by TNF-alpha of SMIT gene expression in 3T3-L1 adipocytes
Satoh et al., Pancreas 1998 (Acute Disease...) : Despite the difference in the iNOS expression, the iNOS messenger RNA ( mRNA ) and the activation of nuclear factor-kappa B (NF-kappa B) were detected in the peritoneal macrophages of both pancreatitis models
Marmiroli et al., FEBS Lett 1998 (Osteosarcoma) : The C-terminal portion of the type I IL-1 receptor ( IL-IRI ) is essential for IL-1 signalling and for IL-1 dependent activation of NF-kappaB
Hamilton et al., J Leukoc Biol 1998 (Inflammation) : Oxidized LDL modulates activation of NFkappaB in mononuclear phagocytes by altering the degradation if IkappaBs
Choi et al., Biochem Pharmacol 1998 : Gel shift retardation analysis showed that LPS substantially activated the hepatic nuclear p65/p50 nuclear factor-kappaB (NF-kappaB) complex with the maximal effect observed at 1 hr at the doses of 1 microg/kg or greater ... LPS induced activation of nuclear NF-kappaB ( 1 microg/kg, i.v. ) failed to be inhibited by concomitant treatment with the mEH and GST inducers, including ADS ( 300 mg/kg, p.o. ), OZ ( 300 mg/kg, p.o. ), and PZ ( 300 mg/kg, i.p. ), indicating that NF-kappaB activation was not required for suppression of the gene expression by LPS ... In contrast, GdCl3, an inhibitor of mEH and GST expression, inhibited LPS induced activation of the p65/p50 NF-kappaB ... In contrast, GdCl3, an inhibitor of mEH and GST expression, inhibited LPS induced activation of the p65/p50 NF-kappaB ... These gel shift analyses provided evidence that LPS induced activation of the NF-kappaB was not responsible for alterations in the gene expression
MacLachlan et al., J Cell Biochem 1998 : Although basal NF-kappaB activity decreases during myogenesis, TNF induced NF-kappaB activity is 10 times greater in myotubes compared with myoblasts, presumably because of the stockpiling of TRAF2 protein in these cells
Lakshminarayanan et al., J Biol Chem 1998 : H2O2 and tumor necrosis factor-alpha induce differential binding of the redox-responsive transcription factors AP-1 and NF-kappaB to the interleukin-8 promoter in endothelial and epithelial cells ... H2O2 activated AP-1 but not NF-kappaB in A549, whereas TNFalpha activated AP-1 as well as NF-kappaB ... In HMEC-1, TNFalpha activated NF-kappaB but not AP-1, while H2O2 did not activate either transcription factor ... TNFalpha induced NF-kappaB complexes containing Rel A ( p65 )
Scarim et al., Endocrinology 1998 (Shock) : IL-1 induced iNOS expression by rat islets requires activation of the transcriptional regulator nuclear factor kappaB (NF-kappaB)
Zhao et al., Alcohol Clin Exp Res 1998 : Neither db-cAMP or 2-mes-ATP affected LPS stimulated NF-kappaB-DNA binding ... Moreover, ETOH and DETC inhibited LPS stimulated NF-kappaB-DNA binding
Guedez et al., J Clin Invest 1998 (Burkitt Lymphoma...) : Furthermore, TIMP-1 upregulation induced expression of Bcl-XL but not Bcl-2 as well as decreased NF-kappaB activity as compared with controls
Lyss et al., J Biol Chem 1998 : The anti-inflammatory sesquiterpene lactone helenalin inhibits the transcription factor NF-kappaB by directly targeting p65
Hu et al., J Biol Chem 1998 : These results suggest that PPX is a novel activator of c-Rel/NF-kappaB
Kirschning et al., J Exp Med 1998 : Bacterial lipopolysaccharide (LPS) induces activation of the transcription factor nuclear factor kappaB (NF-kappaB) in host cells upon infection ... Overexpression of TLR2, but not TLR1, TLR4, or CD14 conferred LPS inducibility of NF-kappaB activation in mammalian 293 cells
Rescigno et al., J Exp Med 1998 : In addition, we found that LPS activated the extracellular signal regulated kinase ( ERK ), and that specific inhibition of MEK1 , the kinase which activates ERK, abrogated the ability of LPS to prevent apoptosis but did not inhibit DC maturation or NF-kappaB nuclear translocation
Hornung et al., Eur J Immunol 1998 (Lymphoma, B-Cell) : At the molecular level, CD40 has been shown to activate nuclear factor kappaB (NF-kappaB) and stress activated protein kinase ( SAPK )
Gukovsky et al., Am J Physiol 1998 (Pancreatitis) : Antioxidant N-acetylcysteine (NAC) blocked NF-kappaB activation and significantly improved parameters of pancreatitis
McDonald et al., Blood 1998 : Interleukin-15 (IL-15) induces NF-kappaB activation and IL-8 production in human neutrophils ... In peripheral blood lymphocytes ( PBL ), however, both IL-2 and IL-15 were potent inducers of NF-kappaB activation ... In peripheral blood lymphocytes ( PBL ), however, both IL-2 and IL-15 were potent inducers of NF-kappaB activation
Galibert et al., J Biol Chem 1998 : An NH2-terminal truncation mutant of TRAF6 inhibited RANKL mediated NF-kappaB activation, but failed to affect constitutive signaling induced by receptor overexpression, revealing a selective role for TRAF6 in ligand induced activation events ... An NH2-terminal truncation mutant of TRAF6 inhibited RANKL mediated NF-kappaB activation, but failed to affect constitutive signaling induced by receptor overexpression, revealing a selective role for TRAF6 in ligand induced activation events
Parrish et al., Toxicol Appl Pharmacol 1998 : In contrast, DNA binding of NF-kappa B was not affected by BaP in either liver or kidney slices
Mohan et al., Invest Ophthalmol Vis Sci 1998 : BMP2 and BMP4 stimulated activation of NF-kappaB ... BMP2 and BMP4 stimulated activation of NF-kappaB ... Actinomycin D and SN50 peptide, but not SN50M control peptide, inhibited NF-kappaB activation in response to BMP2 or BMP4 ... Actinomycin D and SN50 peptide, but not SN50M control peptide, inhibited NF-kappaB activation in response to BMP2 or BMP4
Cheng et al., J Interferon Cytokine Res 1998 : Therefore, effective ISRE activity of IP-10 VRE may require an IRF-like protein binding, which is enhanced by an NF-kappaB heterodimer binding to an adjacent KB site
Lee et al., Eur J Immunol 1998 : ROI have been implicated in the activation of NF-kappaB as well as c-Jun N-terminal kinase (JNK) in response to IL-1 and TNF-alpha stimulation ... ROI have been implicated in the activation of NF-kappaB as well as c-Jun N-terminal kinase (JNK) in response to IL-1 and TNF-alpha stimulation ... Furthermore, transfection of WEHI 231 cells with a plasmid encoding Mn-superoxide dismutase interferes with CD40 induced NF-kappaB activation, providing further support for ROI involvement in this pathway
Saliou et al., FEBS Lett 1998 : Surprisingly, tumor necrosis factor-alpha induced NF-kappaB activation was not affected by silymarin, thus demonstrating a pathway dependent inhibition by silymarin
Held et al., Infect Immun 1999 : We considered the possibility that an interaction between the LPS induced NF-kappaB and IFN-gamma induced JAK-STAT pathways at the pretranscriptional level may enhance the LPS induced signals ... Consistent with the synergistic induction of inducible nitric oxide synthase mRNA and nitric oxide production by a combination of LPS and IFN-gamma, IFN-gamma strongly augmented LPS induced NF-kappaB activation and accelerated the binding of NF-kappaB to DNA to as early as 5 min ... Consistent with the synergistic induction of inducible nitric oxide synthase mRNA and nitric oxide production by a combination of LPS and IFN-gamma, IFN-gamma strongly augmented LPS induced NF-kappaB activation and accelerated the binding of NF-kappaB to DNA to as early as 5 min
Han et al., J Pharmacol Exp Ther 1998 : Under identical condition, we examined the effect of TGF-beta1 on the activity of nuclear factor AT ( NF-AT ), nuclear factor kappaB (NF-kappaB) , activator protein-1 (AP-1) and octamer, all of which contribute to the regulation of IL-2 gene expression ... Electrophoretic mobility shift assays showed that TGF-beta1 markedly increased NF-AT, NF-kappaB and AP-1 binding to their respective cognate DNA binding sites, whereas octamer binding remained constant, as compared with untreated cells
Huxford et al., Cell 1998 : IkappaBalpha regulates the transcription factor NF-kappaB through the formation of stable IkappaBalpha/NF-kappaB complexes
Yang et al., J Nutr 1998 (Disease Models, Animal) : We postulated that green tea polyphenols regulate TNFalpha gene expression by modulating NF-KB activation through their antioxidant properties
Morise et al., J Clin Gastroenterol 1998 (Stomach Diseases) : We have demonstrated that two structurally distinct yet highly selective proteasome inhibitors ( MG341, lactacystin ) inhibit tumor necrosis factor (TNF) induced NFKB activation as well as ECAM expression in human endothelial cells in vitro
Shames et al., Shock 1998 (Endotoxemia) : Evidence suggests that IL-10 may inhibit activation of the transcription factor nuclear factor-kappaB (NF-kappaB) through an unknown mechanism ... The purpose of this study was to determine the effect of IL-10 on lipopolysaccharide (LPS) induced human monocyte TNF-alpha production, NF-kappaB activation, and IkappaB-alpha degradation ... The purpose of this study was to determine the effect of IL-10 on lipopolysaccharide (LPS) induced human monocyte TNF-alpha production, NF-kappaB activation, and IkappaB-alpha degradation ... After LPS stimulation in human monocytes, IkappaB-alpha protein levels decreased, and NF-kappaB DNA binding increased
Cerillo et al., J Steroid Biochem Mol Biol 1998 : Furthermore, the expression of SRC-1alpha or RIP-140 does not enable the ERalpha to repress NFkappaB or to potentiate an AP-1 response in HEK-293 cells
Thomassen et al., J Interferon Cytokine Res 1998 : IL-18 activated NF-kappaB in EL4/6.1 thymoma cells but not in COS-7 cells, even though the latter presumably contain all components required for the IL-1 signaling pathway
Newman et al., J Surg Res 1998 (Heart Diseases) : Release of tumor necrosis factor-alpha from coronary smooth muscle : activation of NF-kappaB and inhibition by elevated cyclic AMP
Sandoval-Chacón et al., Aliment Pharmacol Ther 1998 (Inflammation) : Gene expression of inducible nitric oxide synthase (iNOS) in HT29 cells, direct effects on nitric oxide and peroxynitrite levels, and activation of NF-kappaB in RAW 264.7 cells as influenced by UG were assessed
Eliopoulos et al., J Virol 1999 : In addition, the TRAF2 interacting protein A20 blocked both LMP1 induced JNK and NF-kappaB activation, further implicating TRAF2 in these phenomena ... While expression of a kinase-inactive mutated NF-kappaB inducing kinase (NIK) , a mitogen activated protein kinase kinase kinase which also associates with TRAF2, impaired LMP1 signalling on the NF-kappaB axis, it did not inhibit LMP1 induced JNK activation, suggesting that these two pathways may bifurcate at the level of TRAF2
You et al., J Virol 1999 : As demonstrated by the electrophoretic mobility shift assay, the expression of HCV core protein prolonged or enhanced the TNF-alpha or LT-alpha1beta2 induced NF-kappaB DNA binding activity in HuH-7 and HeLa cells
Maekawa et al., Biochem J 1999 : PTP-BAS may thus play a regulatory role in activation of NF-kappaB under high oxidative stress
Fischer et al., J Immunol 1999 : Both molecules enhanced LPS induced NF-kappaB and activating protein-1 binding activity
Tsubota et al., Invest Ophthalmol Vis Sci 1999 (Sjogren's Syndrome) : IFN-gamma induced the nuclear translocation of NF-kappaB , but did not activate STAT1 or STAT3 in ChWK
Jeremias et al., Eur Cytokine Netw 1998 : TRAIL induces apoptosis and activation of NFkappaB
Wadgaonkar et al., J Biol Chem 1999 : Additionally, tumor necrosis factor activated NF-kappaB is inhibited by UV light induced p53 ... Increased levels of the coactivator relieve p53 mediated repression of NF-kappaB activity and p65 mediated repression of p53 dependent gene expression
Lavie et al., J Biol Chem 1999 : Vascular cell adhesion molecule-1 gene expression during human smooth muscle cell differentiation is independent of NF-kappaB activation ... Since VCAM-1 expression appears developmentally regulated and cytokine-inducible in smooth muscle cells ( SMCs ), we have studied the role of NF-kappaB in differentiated SMC VCAM-1 expression ... Since VCAM-1 expression appears developmentally regulated and cytokine-inducible in smooth muscle cells ( SMCs ), we have studied the role of NF-kappaB in differentiated SMC VCAM-1 expression ... Taken together, these findings suggest that in contrast to TNF-alpha activation, NF-kappaB is not involved in VCAM-1 gene expression during SMC differentiation
Krushel et al., J Biol Chem 1999 : NF-kappaB activity is induced by neural cell adhesion molecule binding to neurons and astrocytes ... These results indicate that N-CAM homophilic binding at the cell membrane leads to increased NF-kappaB binding to DNA and transcriptional activation in both neurons and astrocytes
Georgescu et al., Mol Cell Biol 1999 (Cell Transformation, Neoplastic) : On the other hand, we found that both transcriptional activation of NF-kappaB and activation of PI 3-kinase were significantly suppressed with the F867 mutant receptor, suggesting that the activation of antiapoptotic pathways is the major mechanism for the observed phenotypic difference
Béraud et al., Proc Natl Acad Sci U S A 1999 : The compound had virtually no effect on tumor necrosis factor- and interleukin-1 induced NF-kappaB activities
Jobin et al., Immunology 1998 : Specific NF-kappaB blockade selectively inhibits tumour necrosis factor-alpha induced COX-2 but not constitutive COX-1 gene expression in HT-29 cells ... We used an adenoviral vector containing an NF-kappaB super-repressor ( Ad5IkappaB ) to investigate the role of NF-kappaB in tumour necrosis factor-alpha (TNF-alpha) mediated COX-2 gene expression in a colonic epithelial cell line
Biagioli et al., Free Radic Biol Med 1999 : Despite the effects of NAC on IL-8 elaboration and NF-kappaB activation, RV stimulated increases in supernatant H2O2 were not altered by NAC
Sumitomo et al., J Urol 1999 (Prostatic Neoplasms) : EMSA showed that NF-kappaB inhibitors continuously inhibited TNF-alpha induced NF-kappaB activation
Jiang et al., Science 1999 : Tumor necrosis factor receptor type 1 ( TNF-R1 ) contains a cytoplasmic death domain that is required for the signaling of TNF activities such as apoptosis and nuclear factor kappa B (NF-kappaB) activation
Eaves-Pyles et al., Infect Immun 1999 : The purpose of the present study was to clarify the contribution of microbial invasion to the degradation of the regulatory protein Ikappa Balpha and the subsequent activation of NF-kappaB in cultured intestinal epithelial cells
Lentsch et al., J Immunol 1999 (Acute Disease...) : In the presence of anti-IL-13, activation of the transcription factor , NF-kappaB , was significantly increased in lung ... In the presence of anti-IL-13 , activation of the transcription factor, NF-kappaB , was significantly increased in lung ... These data suggest that during lung inflammation endogenous IL-13 regulates NF-kappaB activation and related cytokine/chemokine generation, all of which determines the intensity of the lung inflammatory response
Lee et al., J Neuroimmunol 1998 : We determined that binding sites for both NF-kappaB ( -186 bp region ) and C/EBP ( -198 bp region ) are involved in TNF-alpha and IL-1beta mediated ICAM-1 upregulation ... We determined that binding sites for both NF-kappaB ( -186 bp region ) and C/EBP ( -198 bp region ) are involved in TNF-alpha and IL-1beta mediated ICAM-1 upregulation
Lentsch et al., Am J Pathol 1999 (Pneumonia) : The presence of SLPI caused greatly reduced activation ( ie, nuclear translocation ) of the transcription nuclear factor-kappaB (NF-kappaB) in lung cells but did not suppress activation of lung mitogen activated protein kinase ... Finally, in the lung inflammatory model used, the presence of anti-SLPI caused accentuated activation of NF-kappaB
Jokelainen et al., Biochem Biophys Res Commun 1998 : The present in vitro study was aimed to clarify whether acetaldehyde has an effect on degradation of IkappaBalpha and activation of NF-kappaB in LPS stimulated rat Kupffer cells ... IkappaBalpha and p65 proteins and activation of NF-kappaB were evaluated
Jaeschke et al., Hepatology 1999 (Endotoxemia...) : However, there was no significant difference in other critical parameters, e.g., activation of the transcription factor , nuclear factor-kappaB (NF-kappaB) , and soluble intercellular adhesion molecule-1 ( sICAM-1 ), parenchymal cell apoptosis, and neutrophil sequestration in the liver
Grisham et al., Methods Enzymol 1999 (Arthritis...) : Inhibition of NF-kappa B activation in vitro and in vivo : role of 26S proteasome
Mejdoubi et al., Biochem Biophys Res Commun 1999 : NF-kappaB is involved in the induction of the rat hepatic alpha1-acid glycoprotein gene by phenobarbital
Riehemann et al., FEBS Lett 1999 (Arthritis, Rheumatoid) : Inhibition of NF-kappaB activation by IDS23 was not mediated by a direct modification of DNA binding, but rather by preventing degradation of its inhibitory subunit IkappaB-alpha
Spencer et al., Oncogene 1999 : However, taxol did not prevent TNF-alpha induced Ikappa-Balpha phosphorylation, degradation, or NF-kappaB activation, indicating that TNF-alpha acts through a microtubule independent pathway
Benya et al., Ann N Y Acad Sci 1998 (Inflammation) : We previously showed that Gal1-R expression was transcriptionally regulated by the transcription factor NF-kappa B
Heyninck et al., FEBS Lett 1999 : The cytokine-inducible zinc finger protein A20 inhibits IL-1 induced NF-kappaB activation at the level of TRAF6 ... The cytokine-inducible zinc finger protein A20 inhibits IL-1 induced NF-kappaB activation at the level of TRAF6 ... Gene induction by IL-1 is mediated by activation of the transcription factor NF-kappaB , and requires the signal adapter protein TRAF6 ... Expression of A20 potently inhibits IL-1 induced NF-kappaB activation by an unknown mechanism ... Inhibition of IL-1 induced NF-kappaB activation was found to be mediated by the C-terminal zinc finger containing domain of A20 ... More importantly, we present evidence that A20 interferes with IL-1 induced NF-kappaB activation at the level of TRAF6, upstream of NIK
Woods et al., Toxicol Appl Pharmacol 1999 : However, in contrast to findings with many other cell types, the activation of NF-kappaB by LPS was not substantially altered either by pretreatment of cells with the thiol antioxidant, N-acetylcysteine, or by glutathione ( GSH ) depletion ... In contrast, LPS activation of NF-kappaB was substantially attenuated by the intracellular Ca2+ chelator, Quin 2AM, and by the Ca-channel inhibitor, ruthenium red ... Additionally, staurosporine, a Ca-dependent protein kinase C inhibitor, substantially decreased LPS mediated NF-kappaB activation
Tsuji et al., Clin Exp Immunol 1999 (Arthritis, Experimental) : Bucillamine and NAC inhibited NF-kappaB activation and tumour necrosis factor-alpha (TNF-alpha) mRNA expression in human monocytic leukaemia cell line THP-1, and cytokine production from monocyte cell lines at concentrations > 10-3 M
Ward et al., J Biol Chem 1999 : In neutrophils, TNF-alpha caused a gliotoxin-inhibitable activation of an inducible form of NF-kappaB , a response that may underlie the ability of TNF-alpha to delay apoptosis at later times ( 12-24 h ) and limit its early killing effect
Gabay et al., Blood 1999 (Liver Neoplasms) : Gel retardation assays showed that IL-4 induced the formation of a STAT6-DNA complex with a STAT6 binding element within the sIL-1Ra promoter, but had no effect on IL-1 induced NF-kappaB binding activity
Wang et al., Invest Ophthalmol Vis Sci 1999 : TNF-alpha , IL-1beta, and TII but not IFN-gamma alone caused degradation of I kappaB, Rel A nuclear translocation, and increased NF-kappaB DNA binding activity, effects that were blocked by pretreatment with MG-132 ... TNF-alpha, IL-1beta, and TII but not IFN-gamma alone caused degradation of I kappaB, Rel A nuclear translocation, and increased NF-kappaB DNA binding activity, effects that were blocked by pretreatment with MG-132 ... TNF-alpha, IL-1beta , and TII but not IFN-gamma alone caused degradation of I kappaB, Rel A nuclear translocation, and increased NF-kappaB DNA binding activity, effects that were blocked by pretreatment with MG-132 ... TNF-alpha, IL-1beta , and TII but not IFN-gamma alone caused degradation of I kappaB, Rel A nuclear translocation, and increased NF-kappaB DNA binding activity, effects that were blocked by pretreatment with MG-132
von Knethen et al., Mol Biol Cell 1999 : Whereas cytokine mediated Cox-2 induction relies on NF-kappaB activation, a low-level NO-elicited Cox-2 response required activation of both NF-kappaB and AP-1
Manna et al., J Immunol 1999 : Within minutes, LPS through CD14 induced the activation of NF-kappaB , degradation of IkappaBalpha ( inhibitory subunit of NF-kappaB ) and IkappaBbeta, and nuclear translocation of p65 ... An antioxidant, pyrrolidine dithiocarbamate, which blocked LPS induced NF-kappaB activation, also abolished the antiapoptotic effects of LPS at the same time ... Besides TNF, the apoptosis induced by taxol and okadaic acid was also sensitive to LPS induced NF-kappaB activation, whereas that induced by H2O2, doxorubicin, daunomycin, vincristine, and vinblastine was NF-kappaB insensitive
Manna et al., J Immunol 1999 : In the present report we demonstrate that treatment of a human T cell line ( Jurkat ) with leflunomide blocks TNF mediated NF-kappa B activation in a dose- and time dependent manner, with maximum inhibition at 5-10 microM ... Leflunomide also suppressed the TNF activated NF-kappa B-dependent reporter gene expression
D'Acquisto et al., Eur J Pharmacol 1999 : We studied the role of nuclear factor-kappaB (NF-kappaB) on the tone and on the expression of inducible nitric oxide (NO) synthase , both evaluated in aortas from lipopolysaccharide treated rats ... We studied the role of nuclear factor-kappaB (NF-kappaB) on the tone and on the expression of inducible nitric oxide (NO) synthase , both evaluated in aortas from lipopolysaccharide treated rats
Cheshire et al., J Biol Chem 1999 : Recently, we demonstrated that IFN-gamma can significantly potentiate TNF-alpha induced nuclear factor (NF)-kappaB nuclear translocation in neuronal derived and endothelial cell lines ... The double stranded RNA dependent kinase PKR is IFN-inducible and has been implicated in the activation of NF-kappaB ; therefore, we examined the possibility that PKR may play a role in the synergistic activation of NF-kappaB during TNF-alpha/IFN-gamma cotreatment ... The PKR inhibitor 2-aminopurine ( 2-AP ) inhibited TNF-alpha/IFN-gamma induced NF-kappaB nuclear translocation in neuronal derived cells but not in endothelial cells ... The PKR inhibitor 2-aminopurine ( 2-AP ) inhibited TNF-alpha/IFN-gamma induced NF-kappaB nuclear translocation in neuronal derived cells but not in endothelial cells ... The PKR inhibitor 2-aminopurine ( 2-AP ) inhibited TNF-alpha/IFN-gamma induced NF-kappaB nuclear translocation in neuronal derived cells but not in endothelial cells
Willis et al., Cell 1999 (Cell Transformation, Neoplastic...) : Bcl10 and E10 activated NF-kappaB but caused apoptosis of 293 cells ... Truncated Bcl10 activated NF-kappaB but did not induce apoptosis
Tsukamoto et al., Proc Natl Acad Sci U S A 1999 : Although four members of the tumor necrosis factor receptor associated factor ( TRAF ) family, including TRAF2, TRAF3, TRAF5, and TRAF6, bind to the CD40 cyt, how each TRAF protein contributes to the NFkappaB activation by CD40 is not clear ... However, single aa substitution of Glu-235 in cyt-N of human CD40 with Ala abolishes the binding of TRAF6 to cyt-N and NFkappaB activation by cyt-N ... Furthermore, NFkappaB activation by cyt-C is inhibited by a kinase negative form of NFkappaB inducing kinase more efficiently than that by cyt-N, consistent with the result that NFkappaB activation by TRAF2 and TRAF5 is inhibited by a kinase negative form of NFkappaB inducing kinase more efficiently than that by TRAF6 ... Furthermore, NFkappaB activation by cyt-C is inhibited by a kinase negative form of NFkappaB inducing kinase more efficiently than that by cyt-N, consistent with the result that NFkappaB activation by TRAF2 and TRAF5 is inhibited by a kinase negative form of NFkappaB inducing kinase more efficiently than that by TRAF6
Lee et al., Proc Natl Acad Sci U S A 1999 (Lymphoma, B-Cell) : Specificities of CD40 signaling : involvement of TRAF2 in CD40 induced NF-kappaB activation and intercellular adhesion molecule-1 up-regulation ... Specificities of CD40 signaling : involvement of TRAF2 in CD40 induced NF-kappaB activation and intercellular adhesion molecule-1 up-regulation ... Overexpression of a dominantly active IkappaBalpha strongly inhibited CD40 induced NF-kappaB activation, ICAM-1 promoter activity, and cell-surface ICAM-1 up-regulation ... Overexpression of a dominantly active IkappaBalpha strongly inhibited CD40 induced NF-kappaB activation, ICAM-1 promoter activity, and cell-surface ICAM-1 up-regulation
Cazals et al., Biochim Biophys Acta 1999 (Hyperoxia) : Role for NF-kappa B in mediating the effects of hyperoxia on IGF binding protein 2 promoter activity in lung alveolar epithelial cells ... From examination of the IGFBP-2 gene promoter sequence which revealed the presence of four potential binding sites for transcription factors of the NF-kappa B/Rel family, we hypothesized that NF-kappa B might be involved in the transcriptional activation of IGFBP-2 in oxidant exposed cells ... Data reported herein demonstrated that NF-kappa B activated IGFBP-2 promoter in transient transfection assays, and that exposure of cells to hyperoxia was associated with accumulation of the active form of NF-kappa B
Ohkawa et al., Biochim Biophys Acta 1999 : In TNF-alpha stimulated MRC-5 cells, DNA binding and competition experiments indicated that the predominant NF-kappa B binding activity detected with nuclear extract stimulated cells is mediated by the p50/p65 complex ... Using antisense oligonucleotides, we confirmed that the TNF-alpha-stimulation of HA synthesis by MRC-5 cells is dependent on the activation of the p50/p65 NF-kappa B complex
Giardina et al., Biochim Biophys Acta 1999 (Colorectal Neoplasms) : Cell death does correlate with alterations in NF-kappa B activity : the NSAIDs, butyrate and H2O2 enhance c-Rel complex formation by TNF-alpha and provide an overall enhancement of NF-kappa B activation by Fas
Abate et al., Prostaglandins Other Lipid Mediat 1998 : Our results show that induction of COX-2 by LPS in macrophages involves activation of NF-kappa B and point to a possible therapeutic use of protease inhibitors in inflammatory processes